gebhart/patrepeval huggingface dataset:
HIV-1 protease inhibitors decrease proliferation and induce differentiation of human myelocytic leukemia cells (2000)

Inhibitors of the protease of human immunodeficiency virus type 1 (HIV-1) may inhibit cytoplasmic retinoic acid-binding proteins, cytochrome P450 isoforms, as well as P-glycoproteins. These features of the protease inhibitors might enhance the activity of retinoids. To explore this hypothesis, myeloid leukemia cells were cultured with all-trans retinoic acid (ATRA) either alone or in combination with the HIV-1 protease inhibitors indinavir, ritonavir, and saquinavir. Consistent with the hypothesis, the HIV-1 protease inhibitors enhanced the ability of ATRA to inhibit growth and induce differentiation of HL-60 and NB4 myeloid leukemia cells, as measured by expression of CD11b and CD66b cell surface antigens, as well as reduction of nitroblue tetrazolium. Growth of ATRA-resistant UF-1 cells was also inhibited when cultured with the combination of ATRA and indinavir. Moreover, indinavir enhanced the ability of ATRA to induce expression of the myeloid differentiation-related transcription factor C/EBPε messenger RNA in NB4 cells by 9.5-fold. Taken together, the results show that HIV-1 protease inhibitors enhance the antiproliferative and differentiating effects of ATRA on myeloid leukemia cells. An HIV-1 protease inhibitor might be a useful adjuvant with ATRA for patients with acute promyelocytic leukemia and possibly retinoid-resistant cancers.
Automated Identification of Chromosome Segments Involved in Translocations by Combining Spectral Karyotyping and Banding Analysis (2008)

The identification of chromosome abnormalities is an essential part of diagnosis and treatment of genetic disorders such as chromosomal syndromes and many types of cancer. Modern cytogenetic imaging techniques have improved the study of chromosome aberrations but they are most often used as adjuncts to traditional G-banded karyotype analysis. Molecular cytogenetic techniques such as comparative genomic hybridization, multicolor fluorescence <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">in</i> <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">situ</i> hybridization, and spectral karyotyping (SKY) are able to detect chromosome copy number changes and complex structural aberrations in cancers, particularly in hematological malignancies and solid tumors. However, banded chromosome analysis is essential to distinguish between normal and abnormal chromosomes. Currently available cytogenetic imaging software is designed to classify only normal chromosomes. The identification of the banded regions involved in the abnormal chromosomes is done manually. In this paper, we propose an algorithm to automate the banding analysis of abnormal chromosomes by comparing the information obtained by SKY for precise identification of translocation break points. Our algorithm is based on the dynamic time warping method in order to overcome the problems due to the nonrigid nature of chromosomes. The method has been implemented and successfully applied to detect chromosome translocations, deletions, and duplications in cell lines derived from solid tumors.
[Radioimmunoassay for pituitary hormones]. (1973)

nese). Radioimmunoassays for thyroid-stimulating hormone (TSH), luteinizing hormone (LH), follicle-stimulating hormone (FSH) and adrenocorticotrophic hormone (ACTH) of anterior pituitary hormones were examined. TSH, FSH and LH were labeled with /sup 131 I/ by the Hunter and Greenwood method, and the resulting radioactivities were measured after separating bound and free hormones by the double antibody technique. As for the reproducibility of this method, the coefficient of variation was 18.3 to 23.3% for different assays for TSH and 6.5 to 9.0% for the same assay. The coefficient of variation in different assays for LH was 15% and for FSH was 17% and that in the same assay for both was 5%. The characteristics of this method were that the effects of other pituitary hormones on the measured hormone were few and the dilution curve of the serum was parallel to or coincided with the standard curve in the measurement of the serum samples. The recovery rate of /sup 131/I was 75 to 110%, when standard TSH was added to the serum of patients deficient in TSH. The levels of TSH, LH and FSH in the blood of patients with panhypopituitarism were less than the sensitivity of the measurement by this method. Inmore » primary hypothyroidism TSH level was high, and in primary hypogenitalism LH and FSH levels also were high. TSH in the blood increased after the administration of antithyroid agents and decreased after the administration of thyroid hormones. LH and FSH in the blood increased after the administration of gonadotropin. TSH in the blood increased markedly by the intravenous injection of 50 to 80 mu g TRH, which specifically secreted TSH, and LH and FSH in the blood increased by 100 mu g of LH releasing hormone. Characteristic variations of LH and FSH were concomitant with menstrual cycle variations. The measurement values by this method were well correlated with the values by bioassay, but the absolute values were higher for bioassay values, although the reason was unknown. (Japan)« less
Object Oriented Design and Patterns (2007)

Introduces object-oriented fundamentals critical to designing software and shows how to implement design techniques Horstmanns clear, hands-on presentation and outstanding writing style help readers to better understand the material Useful sidebars cover relevant data structures C++ notes throughout the book illustrate object-oriented programming concepts that are not available with Java An online UML editor (designed by the author) allows readers to create and test UML code Also available from Cay Horstmann, the highly successful Computing Concepts with Java Essentials, Third Edition (0-471-24371-X); Computing Concepts with C++ Essentials, Third Edition (0-471-16437-2), and Big Java (0-471-40248-6)
Calibration procedures of test dummies for side impact testing. Final report (1977)

Notes: Report covers the period 20 Nov 1975 - 31 Oct 1976. Contract amount - $41,660. Release date - March 1978
Effective textile printing using different enzyme systems (2005)

The possibility of using enzymes in natural and synthetic thickener systems for the printing of cotton and wool has been investigated, in particular the effect on colour and surface structure. The efficiency of different enzymes (cellulases, proteases and laccases) concerning their applicability, i.e. activity and stability within different thickener systems (polysaccharide, acrylic polymer and their mixtures), was studied. Rheological parameters (viscosity and viscoelasticity) of the printing paste were determined and the colour and/or structure effects on the fabric surfaces were assessed. The rheological investigations showed that degradation of the structure of the thickener system could occur in the case of an inappropriate combination of thickener and enzyme product leading to pseudoplasticity and decreased elasticity of the paste.
Crystallization Studies of Epigallocatechin Gallate (2007)

Flavonoids are a long and well known class of natural products. Their potential health benefits can be attributed to their antioxidant activity, and modulation of cell signaling pathways. Green tea one of the most widely consumed beverages, consist of flavonoids such as catechins and tannins. Epigallocatechin gallate (EGCG) the major catechin of green tea exhibits multiple health benefits due to its antioxidant nature. The radical scavenging activity of EGCG is attributed to its structure. Therefore, a study on molecular features of EGCG would provide valuable information on structural modifications, which may change the physiochemical properties such as bioavailability and solubility. Although flavonoids are abundant and commercially available they are difficult to purify and crystallize. In this respect, crystallizing EGCG was challenging. By exploring different techniques EGCG was crystallized. Here in this study one new form of EGCG and two solvates, acetonitrile and nitrobenzene, have been synthesized and structurally characterized by differential scanning calorimetry (DSC), infrared (IR) and powder X-ray diffraction (PXRD). The crystal structures were solved by single-crystal X-ray diffraction and a detailed description of synthesis and about the supramolecular synthons that exist in these crystal forms are given.
Improvement of transmitter identification system for low SNR transients (2004)

Performance of radio transmitter identification systems for low SNR transients can be improved by utilising the transient SNR information in the classifier training stage. The SNR of the unknown transient, which determines the amount of noise to be added into the neural network training set, has been estimated at the classification stage using pre- and post-trigger samples of the captured noisy transient signal. Performance improvement observed in simulations is verified with experimental data.
Smart Phone based Snoring Sound analysis to Identify Upper Airway Obstructions (2019)

Obstructive sleep apnea (OSA) is characterized by upper airway obstructions known as apnea/hypopnea events. Narrowing of the upper airway during or near the vicinity of apnea/hypopnea causes the spectrum of the snores to shift to higher frequencies. Using an instrumentation quality wideband (WB) microphone (4Hz-100kHz), we previously demonstrated that potentially diagnostically useful frequency shifts could be detected even in regions beyond the human hearing range. WB-microphone based systems are expensive and not available for home use or population screening application. In this paper we explore the feasibility of using smart phones to analyze snoring sounds in the 20Hz-22kHz band to identify events of upper airway obstructions. Modern smart phones have internal microphones with bandwidths up to 22kHz, above the nominal human hearing range, and provide a good platform for sound acquisition and processing. For the work of this paper we used a Samsung Galaxy S3 phone and recorded overnight respiratory sound data from 8 patients undergoing routine Polysomnography (PSG) study in a hospital. Our target was to develop models to classify each standard 30 second epoch of data as non-apnea or apnea. Using 700 epochs we developed logistic regression models with the input as snoring sound features and the outputs as the diagnostic classification of each event (apnea/non-apnea). Models developed within a 20Hz-15kHz band had accuracies of 89-93%, sensitivities 70-78% and kappa index ranging 0.75-0.83 on validation data set. When the same models were developed on the 20Hz-22kHz frequency band the improved performance shows accuracies 94- 97%, sensitivities 93-100%, and kappa ranging 0.86-0.91. The study shows that smart phones based high frequency band (15-22kHz) of snoring sounds carry information about the upper airway obstructions. Our non-contact, smart phone based snoring sound technology has potential to identify upper airway obstructions.
Comparison of Histologic Core Portions Acquired from a Core Biopsy Needle and a Conventional Needle in Solid Mass Lesions: A Prospective Randomized Trial (2017)

The proportion of histologic core and the diagnostic accuracy were comparable between the FNB and FNA groups. However, fewer needle passes were required to establish an accurate diagnosis in EUS-FNB.
Controlling Seizure-Like Events by Perturbing Ion Concentration Dynamics with Periodic Stimulation (2013)

We investigate the effects of adding periodic stimulation to a generic, conductance-based neuron model that includes ion concentration dynamics of sodium and potassium. Under conditions of high extracellular potassium, the model exhibits repeating, spontaneous, seizure-like bursting events associated with slow modulation of the ion concentrations local to the neuron. We show that for a range of parameter values, depolarizing and hyperpolarizing periodic stimulation pulses (including frequencies lower than 4 Hz) can stop the spontaneous bursting by interacting with the ion concentration dynamics. Stimulation can also control the magnitude of evoked responses to modeled physiological inputs. We develop an understanding of the nonlinear dynamics of this system by a timescale separation procedure that identifies effective nullclines in the ion concentration parameter space. Our results suggest that the manipulation of ion concentration dynamics via external or endogenous stimulation may play an important role in neuronal excitability, seizure dynamics, and control.
Emergent construction of a behavior arbitration mechanism based on the immune system (1997)

Abstract We have been investigating a new behavior arbitration mechanism based on the biological immune system. The behavior arbitration mechanism and the biological immune system share certain similarities since both systems deal with various sensory inputs (antigens) through interactions among multiple competence modules (lymphocytes and/or antibodies). We have demonstrated the flexible arbitration abilities of our proposed method; however, we have not shown a solution to the problem: how do we prepare an appropriate repertoire of competence modules? In this paper, in order to construct an appropriate immune network without human intervention, we try to incorporate an offline metadynamics function into our previously proposed mechanism. The metadynamics function is an adaptation realized by varying the structure of the immune network. To accomplish this function, we use a genetic algorithm with a devised crossover operator. Finally, we verify our method by carrying out simulations.
Clinical Predictors for Failure of Percutaneous Coronary Intervention in ST‐elevation Myocardial Infarction (2012)

In patients with STEMI, primary PCI failure is uncommon. It is associated with worse short- and long-term clinical outcome compared with a successful procedure. Special care should be taken when PCI is performed in women at higher risk for failure when presenting with STEMI.
L'accord franco-italien et la situation des Italiens en Tunisie (1935)

Célérier Jean. L'accord franco-italien et la situation des Italiens en Tunisie. In: Annales de Géographie, t. 44, n°249, 1935. Buenos Aires - Étude de géographie urbaine. pp. 328-329.
Effect of Pharmacological Blockade of ACTH and TSH Secretion on the Acute Stimulation of Prolactin Release by Exposure to Cold and Ether Stress (1976)

Acute exposure of male rats to cold (5C)leads to a rapid increase of plasma levels of thyrotropin (TSH), prolactin (PRL), corticosterone, and L-thyroxine. Exposure to ether is similarly followed by a rapid increase of plasma levels of PRL and corticosterone, while TSH release is inhibited. Acute treatment with dexamethasone (500 mug) inhibits almost completely the PRL response to both exposure to cold and ether stress, while the plasma TSH response to cold is only delayed and the decrease of plasma TSH observed after ether stress is unchanged. Basal plasma levels of both TSH and PRL are lowered after treatment with the steroid. Thyroxine treatment lowers the plasma TSH concentration to undetectable levels without affecting the plasma PRL response to cold or ether exposure. The present data suggest that the rise of plasma PRL observed after cold exposure is not related to TRH and may suggest that common mechanisms control ACTH and PRL secretion during acute stress exposure.
Cysteine Oxidation in the Mitogenic S100B Protein Leads to Changes in Phosphorylation by Catalytic CKII-α Subunit (1998)

The glial-derived calcium-binding protein S100B can be secreted to act as a neurotrophic factor or a mitogen, stimulating proliferation of glial cells. The extracellular S100B activities rely on the oxidation of the protein cysteine residues (Kligman, D., and Marshak, D. R. (1985) Proc. Natl. Acad. Sci. U. S. A. 82, 7136-7139; Winningham-Major, F., Staecker, J. L., Barger, S. W., Coats, S., and Van Eldik, L. J. (1989) J. Cell Biol. 109, 3063-3071). Here we show that oxidation of the S100B cysteine residues, Cys-68 and Cys-84, induces a conformational change in the protein structure, unmasking a canonical CKII phosphorylation site located within the typical EF-hand calcium-binding site IIbeta. Intrasubunit disulfide-bridged S100B monomer and disulfide-bonded S100B dimer are phosphorylated by the catalytic CKII-alpha subunit on Ser-62 with a Km of 0.5 microM and a Vmax of 10 pmol/min/100 pmol of S100B. Oxidized S100B is the best in vitro CKII-alpha substrate identified so far. Next we show that intrasubunit disulfide-bridged S100B monomer is the most potent S100B species to stimulate [3H]thymidine uptake by C6 glial cells in culture. In addition, the phosphorylated intrasubunit disulfide-bridged S100B monomer retains apparent mitogenic activity toward C6 glial cells, and hence, 32P-labeled S100B should be a useful probe for characterizing the mechanisms by which extracellular oxidized S100B functions. Finally, we show that formation of intrasubunit disulfide-bridged S100B monomer is stimulated by peroxynitrite anion, suggesting that production of mitogenic S100B species could be enhanced in neuropathology associated with peroxynitrite anion production.
ChemInform Abstract: Structure Determination of the Products from the Acid‐Catalyzed Condensation of Indole with Acetone. (1996)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Genetic Diversity of <i>Borrelia burgdorferi</i> in Lyme Disease Patients as Determined by Culture versus Direct PCR with Clinical Specimens (1999)

Two hundred seventeen isolates of Borrelia burgdorferi originally cultured from skin biopsy samples or blood of early Lyme disease patients were genetically characterized by PCR-restriction fragment length polymorphism (RFLP) typing of the 16S-23S ribosomal DNA intergenic spacer. Three major RFLP types were observed. Of the cultured isolates, 63 of 217 (29.0%) were type 1, 85 of 217 (39.2%) were type 2, and 58 of 217 (26.7%) were type 3; mixtures of two RFLP types were obtained in 6.0% (13 of 217) of the cultures. Comparison of typing of B. burgdorferi performed directly on 51 patient skin specimens with typing of cultures originally isolated from the same tissue revealed that a much larger proportion of direct tissue samples had mixtures of RFLP types (43.1% by direct typing versus 5.9% by culture [P < 0.001). In addition, identical RFLP types were observed in only 35.5% (11 of 31) of the paired samples. RFLP type 3 organisms were recovered from blood at a significantly lower rate than were either type 1 or type 2 strains. These studies demonstrate that the genetic diversity of B. burgdorferi patient isolates as determined by cultivation differs from that assessed by PCR performed directly on patient tissue.
Combining MONSSTER and LES/PME to Predict Protein Structure from Amino Acid Sequence:  Application to the Small Protein CMTI-1 (2000)

A combined method for the prediction of protein tertiary structures from sequence is presented. This multistep procedure initially uses a simplified approach to protein structure prediction, MONSSTER, that assembles structures from initial extended conformations and scores them. Then, using the lowest-energy low-resolution model as a starting conformation, a detailed atomic model is built and refined using molecular dynamics simulations that employ the locally enhanced sampling (LES) methodology with the particle mesh Ewald (PME) technique for calculation of long-range electrostatic interactions. The combined method is applied to a small disulfide-rich 29-residue protein CMTI-1, a trypsin inhibitor found in squash seeds. Starting with an initial low-resolution model from MONSSTER, which has an rmsd from the native conformation of 3.7 Å (5.0 Å) for Cα atoms (all heavy atoms), LES/PME refinement leads to a structure that is only 2.5 Å (3.3 Å) from native, with a Cα rmsd of only 1.7 Å for residues 5−29. These rmsd values should be compared to Cα rmsd values of 1.2 Å (all residues) or 0.8 Å (residues 5−29) found in PME molecular dynamics simulations that start with the native conformation.
Enhanced Cartilage Formation via Three-Dimensional Cell Engineering of Human Adipose-Derived Stem Cells (2012)

Autologous chondrocyte implantation is an effective treatment for damaged articular cartilage. However, this method involves surgical procedures that may cause further cartilage degeneration, and in vitro expansion of chondrocytes can result in dedifferentiation. Adipose-derived stem cells (ADSCs) may be an alternative autologous cell source for cartilage regeneration. In this study, we developed an effective method for large-scale in vitro chondrogenic differentiation, which is the procedure that would be required for clinical applications, and the subsequent in vivo cartilage formation of human ADSCs (hADSCs). The spheroid formation and chondrogenic differentiation of hADSCs were induced on a large scale by culturing hADSCs in three-dimensional suspension bioreactors (spinner flasks). In vitro chondrogenic differentiation of hADSCs was enhanced by a spheroid culture compared with a monolayer culture. The enhanced chondrogenesis was probably attributable to hypoxia-related cascades and enhanced cell-cell interactions in hADSC spheroids. On hADSCs loading in fibrin gel and transplantation into subcutaneous space of athymic mice for 4 weeks, the in vivo cartilage formation was enhanced by the transplantation of spheroid-cultured hADSCs compared with that of monolayer-cultured hADSCs. This study shows that the spheroid culture may be an effective method for large-scale in vitro chondrogenic differentiation of hADSCs and subsequent in vivo cartilage formation.
Analysis of Risk Factors for Cisplatin-Induced Ototoxicity in Patients With Testicular Cancer (1998)

No AccessJournal of UrologyUrological Survey: Abstracts: Diagnostic Urology and Testis Cancer1 Dec 1998Analysis of Risk Factors for Cisplatin-Induced Ototoxicity in Patients With Testicular Cancer B. Bokemeyer, C.C. Berger, J.T. Hartmann, C. Kollmannsberger, H.-J. Schmoll, M.A. Kuczyk, and L. Kanz B. BokemeyerB. Bokemeyer More articles by this author , C.C. BergerC.C. Berger More articles by this author , J.T. HartmannJ.T. Hartmann More articles by this author , C. KollmannsbergerC. Kollmannsberger More articles by this author , H.-J. SchmollH.-J. Schmoll More articles by this author , M.A. KuczykM.A. Kuczyk More articles by this author , and L. KanzL. Kanz More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(01)62317-6AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail "Analysis of Risk Factors for Cisplatin-Induced Ototoxicity in Patients With Testicular Cancer." The Journal of Urology, 160(6 Part 1), pp. 2292–2293 Department of Hematology/Oncology/Immunology, Eberhard-Karls-University Medical Center II, Tuebingen, Department of Hematology/Oncology, Martin-Luther-University, Halle/Wittenberg and Department of Urology, University Medical School, Hannover, Germany© 1998 by American Urological Association, Inc.FiguresReferencesRelatedDetails Volume 160Issue 6 Part 1December 1998Page: 2292-2293 Advertisement Copyright & Permissions© 1998 by American Urological Association, Inc.MetricsAuthor Information B. Bokemeyer More articles by this author C.C. Berger More articles by this author J.T. Hartmann More articles by this author C. Kollmannsberger More articles by this author H.-J. Schmoll More articles by this author M.A. Kuczyk More articles by this author L. Kanz More articles by this author Expand All Advertisement PDF downloadLoading ...