gebhart/patrepeval huggingface dataset:
FIRST INSIGHTS INTO IMPROVEMENT OF EICOSAPENTAENOIC ACID CONTENT IN <i>PHAEODACTYLUM TRICORNUTUM</i> (BACILLARIOPHYCEAE) BY INDUCED MUTAGENESIS<sup>1</sup> (1996)

ABSTRACT A strain improvement program was initiated based on mutagenesis with the goal of commercial production of eicosapentaenoic acid (EPA)from EPA‐overproducing microalgal strains. Two rounds of mutation and selection were conducted using Phaeodactylum tricornutum Bohlin UTEX #640 as the parent strain. After the first round of mutagenesis, a putative mutant (provisionally labeled 114) was obtained. The EPA content (% of dry weight) of this mutant strain was 37% higher than that of the wild type. 114 was further mutated and another putative mutant (provisionally called II242) was isolated, the EPA content of which was 44% higher than that of the wild type. When cultured with aeration in 1‐L flasks, EPA content of the wild type and putative mutants 114 and II242 was, 17.3 mg · g −1 , 31.5mg · g −1 , and 38.6 mg · g −1 dry biomass, respectively. EPA productivity was 3.48 mg · L −1 · d −1 4.01 mg · L −1 · d −1 , and 4.98 mg · L −1 · d −1 respectively. These figures compare favorably with many other promising EPA‐producing microorganisms and suggest that the use of a single methodology such as mutation and selection is a way to improve the polyunsaturated fatty acid content of microalgae and other microorganisms.
Exposição ocupacional a material biológico contaminado por HIV : abordagem fenomenológica (2012)

Made available in DSpace on 2021-05-14T23:04:03Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-06-29
HIV-infected patients display increased proatherogenic anti-apolipoprotein A1 autoantibodies, inflammatory and metabolomic markers (2021)

Abstract Background HIV-infected patients display an increased risk of cardiovascular events. HIV and antiretroviral therapy (ART) have both been suggested to increase this risk but their specific role remains unclear. Autoantibodies against apolipoprotein A1 (anti-apoA1 IgG) are also associated with cardiovascular risk. The aim of this study was to analyse anti-apoA1 IgG levels and inflammatory markers as well as the metabolomic profile in a cohort of HIV-free, ART-experienced and naïve HIV-infected patients. Methods 144 participants from a South African cohort were categorised into HIV-free (n=50), HIV-infected/ART-experienced (n=50) and HIV-infected/ART-naïve (n=44) groups. The levels of anti-apoA1 IgG were assessed by homemade ELISA assays. We performed targeted metabolomic analyses using standard operating procedures and measured inflammatory biomarkers using the Meso Scale Discovery® platform. Additionally, we conducted in vitro experiments on human aortic endothelial cells treated with anti-apoA1 IgG and assessed levels of adhesion molecules. Results Inflammatory markers (IFNg, IL-6, IL-10, TNFa, CRP all p&lt;0.05), circulating adhesion molecules (ICAM-1 and VCAM-1, p&lt;0.05), anti-apoA1 IgG levels were increased in HIV-infected patients compared to HIV-free participants. While ART treatment significantly reduced IFNg, IL-10, TNFa, VCAM-1, anti-apoA1 IgG levels, no significant differences were observed in other biomarkers between ART-experienced and naïve participants. Treatment of human aortic endothelial cells with anti-apoA1 IgG (40μg/ml) induced a significant increase in these adhesion molecules compared to cells treated with control IgG (40μg/ml). Metabolomic analyses identified 257 endogenous circulating metabolites in plasma. Among these metabolites, we found that in HIV-free and infected patients 14 and 17 metabolites were significantly correlated with anti-apoA1 IgG levels, respectively. Among these 17 metabolites, 2 were positively correlated with the tryptophan pathway; kynurenine (r=0.292, p=0.0069) and 5-hydroxyindolacetate (r=0.316, p=0.0019). Interestingly, kynurenine was not significantly correlated with anti-apoA1 IgG levels in HIV-free patients. Conclusion HIV-infected patients displayed a more proatherogenic profile compared to HIV-free subjects. Anti-apoA1 IgG induced adhesion molecules expression. Metabolomics identified metabolites that were significantly associated with anti-apoA1 IgG levels and inflammatory biomarkers known to underlie atherosclerosis burden. Funding Acknowledgement Type of funding sources: None.
Synthesis and delivery of short, noncoding RNA by B lymphocytes (2013)

Evolutionarily conserved short (20-30 nucleotides) noncoding RNAs (microRNAs) are powerful regulators of gene expression in a variety of physiological and pathological processes. As such, means to efficiently modulate microRNA function constitute an important therapeutic opportunity. Here we demonstrate that primary B lymphocytes can be genetically programmed with nonviral plasmid DNA for the biogenesis and delivery of antisense sequences (anti-microRNA) against microRNA-150 (miR-150). Within 18 h of transfection with an anti-miR-150 construct, primary B lymphocytes secrete ∼3,000 copies of anti-miR-150 molecules per cell. Anti-miR-150 molecules released by B lymphocytes were internalized by CD8 T lymphocytes during cross-priming in vitro and in vivo, resulting in marked down-regulation of endogenous miR-150. However, such internalization was not observed in the absence of cross-priming. These results suggest that shuttling anti-miR-150 molecules from B lymphocytes to T cells requires the activation of receiver T cells via the antigen receptor. Finally, anti-miR-150 synthesized in B cells were secreted both as free and extracellular vesicle-associated fractions, but only extracellular vesicle-associated anti-miR-150 were apparently taken up by CD8 T cells. Collectively, these data indicate that primary B lymphocytes represent an efficient platform for the synthesis and delivery of short, noncoding RNA, paving the way for an approach to immunogenomic therapies.
Lipid particles/droplets of the yeast Saccharomyces cerevisiae revisited: Lipidome meets Proteome (2011)

In the yeast Saccharomyces cerevisiae as in other eukaryotes non-polar lipids are a reservoir of energy and building blocks for membrane lipid synthesis. The yeast non-polar lipids, triacylglycerols (TG) and steryl esters (SE) are stored in so-called lipid particles/droplets (LP) as biologically inert form of fatty acids and sterols. To understand LP structure and function in more detail we investigated the molecular equipment of this compartment making use of mass spectrometric analysis of lipids (TG, SE, phospholipids) and proteins. We addressed the question whether or not lipid and protein composition of LP influence each other and performed analyses of LP from cells grown on two different carbon sources, glucose and oleate. Growth of cells on oleate caused dramatic cellular changes including accumulation of TG at the expense of SE, enhanced the amount of glycerophospholipids and strongly increased the degree of unsaturation in all lipid classes. Most interestingly, oleate as a carbon source led to adaptation of the LP proteome resulting in the appearance of several novel LP proteins. Localization of these new LP proteins was confirmed by cell fractionation. Proteomes of LP variants from cells grown on glucose or oleate, respectively, were compared and are discussed with emphasis on the different groups of proteins detected through this analysis. In summary, we demonstrate flexibility of the yeast LP lipidome and proteome and the ability of LP to adapt to environmental changes.
Distribution of basal ganglia lesions in Pick's disease with Pick bodies: A topographic neuropathological study of eight autopsy cases (1999)

The distribution of the basal ganglia lesions, including the amygdala, striatum, and pallidum, were investigated neuropathologically in eight Japanese autopsy cases of Pick's disease with Pick bodies. The lesions were classified as mild, moderate or severe. The degree and distribution of basal ganglia lesions in all eight cases were uniform: the amygdala showed severe to moderate lesions, the caudate nucleus and putamen showed moderate to mild lesions, and the pallidum showed mild lesions. Furthermore, the lesions in the amygdala were more prominent in the basolateral group than in the corticomedial group. In Pick's disease with Pick bodies, the degree and distribution of the lesions within the basal ganglia differs from those reported in both ‘Pick's disease without Pick bodies’ and corticobasal degeneration (CBD), in which severe lesions were present in the pallidum. These neuropathological findings may contribute to the morphological differential diagnosis among Pick's disease with Pick bodies, ‘Pick's disease without Pick bodies’, and CBD.
Models of multiprocessor computing (2002)

Today's options for high-performance computing include message-passing MPP systems, clusters of workstations, shared-memory multiprocessors, and parallel vector processors. Shared-memory multiprocessors provide the most general-purpose programming model, but generally suffer from limited scalability. New multiprocessor cache coherence protocols together with high-performance CMOS VLSI and interconnects are enabling a new class of machine that scales the shared-memory paradigm. The author introduces the scalable shared-memory multiprocessor (SSMP) and SGI's new Origin servers based on this technology. The Origin machines are highly modular and permit a single system to grow from a cost-effective deskside uniprocessor to a supercomputer with hundreds of processors and hundreds of gigabytes of shared-memory. Nodes within the system provide a peak of 50 GB/sec of memory bandwidth and are connected by fat hypercube network that has a peak bisection bandwidth of 25 GB/sec. These capabilities also enable new levels of I/O performance including file servers with sustained bandwidths in excess of 500 MByte/sec and networking interfaces such as SuperHIPPI that can source and sink data at up to 800 MB/sec simultaneously. He concludes the talk with a look at the future of multiprocessing. He predicts a merger of the HPC computing models on SSMP and clusters of SSMP systems.
Freeform modeling of faulted surfaces in seismic images (2009)

An interactive surface editing framework that is well suited to the needs of geophysical modelers and interpreters is described. This surface editing framework allows a non‐expert user to quickly create complicated surfaces by combining two simple concepts: deformation and cutting. Deformations change the geometry of a surface and allow a user to model continuous geologic horizons. Cutting changes the topology of the surface to model geologic faults.
Hormone replacement therapy in healthy postmenopausal women: a randomized, placebo-controlled study of effects on coagulation and fibrinolytic factors (2001)

Unopposed oestrogen substitution was associated with both potentially beneficial effects, such as decreases in fibrinogen, and potentially thrombogenic effects such as decreasing AT III and protein S and increasing FVII. During prolonged follow-up and addition of progesterone, differences between groups concerning FVII were attenuated. These data suggest that effects of HRT upon coagulation are most pronounced early after institution of unopposed treatment.
Timing analysis of First-Come First-Served scheduled interval-timed Directed Acyclic Graphs (2014)

Analyzing worst-case application timing for systems with shared resources is difficult, especially when non-monotonic arbitration policies like First-Come-First-Served (FCFS) scheduling are used in combination with varying task execution times. Analysis methods that conservatively analyze these systems are often based on state-space exploration, which is not scalable due to its inherent susceptibility to combinatorial explosion. We propose a scalable timing analysis method on periodically restarted Directed Acyclic Task Graphs, that can provide conservative bounds on task timing properties when shared resources with FCFS scheduling are used. By expressing task enabling and completion times in intervals, denoting best-case and worst-case timing properties, contention on the shared resources can be estimated using conservative approximations. With an industrial case study we show that our approach can easily analyze models with thousands of tasks in less than 10 seconds, and the worst-case bounds obtained show an average improvement of 46% compared to bounds obtained by static worst-case analysis.
New Multicomponent Capsules for Immunoisolation (1999)

A new generation of microcapsules based on the use of oligomers which participate in polyelectrolyte complexation reactions has been developed. These freeze-thaw stable capsules have been applied as a bioartificial pancreas and have resulted in normoglycemia for periods of six months in concordant xenotransplantations. The new chemistry permits the control of permeability and mechanical properties over a wide range and can be adapted both to microcapsule and hollow fiber geometries rendering it a robust tool for encapsulation in general. Methods, and metrics, for the characterization of the mechanical properties and permeability of microcapsules are presented.
A Toolbox for Input-Output System Inversion (2007)

In this paper a Matlab-based toolbox for the input-output system inversion of linear systems is presented. Different methods, based either on analytical or numerical approaches, are implemented. The toolbox can be exploited in the design of a feedforward action for control systems in different contexts in order to improve performances in the set-point regulation. The use of a pre-actuation and a post-actuation time can be easily analyzed as well as the role played by the choice of the desired output function.
Inheritance of Male Sterility in Flax Involving Nuclear‐Cytoplasmic Interaction, Including Methods of Testing for Cytoplasmic Restoration<sup>1</sup> (1981)

An interaction between cytoplasm and nuclear genes affecting the inheritance of male sterility in flax ( Linum usitatissimum L.) was reported first by workers in England in the 1920s in crosses between procumbent and tall plants and later by a worker in Poland using Linum floccosum . Previously unpublished data are reported on a similar case involving a large‐seeded flax from Crete. The interaction was discovered in all cases by noting that crosses using procumbent, L. floccosum , and Crete as female parents with most other stocks as male parents had fertile and male‐sterile plants in F 2 ; whereas the reciprocal crosses had only fertile plants. For certain crosses using Crete as the female parent, the F 2 segregation ratios were for a single gene difference. Other crosses may have involved two genes complementary either for restoration of pollen fertility in F 1 or for incomplete restoration. Either of two hypotheses will explain the difference in behavior between reciprocal crosses. One hypothesis is that procumbent, L. floccosum , and Crete have a dominant fertility allele, Ms , and a non‐restorer [N] cytoplasm. Most other stocks have the recessive male‐sterile, ms ms , nuclear genotype but a cytoplasm [c R] that restores fertility. The cross of male steriles [N] ms ms with maintainer [c R] ms ms stocks would produce all male‐sterile progeny. Crossing these male steriles with Ms Ms would produce hybrid seed. The second hypothesis that may explain the difference in behavior between reciprocal crosses assumes that procumbent, L. floccosum , and Crete have cytoplasmic male sterility [cms] together with a nuclear gene for restoration of pollen fertility ( Rf ). Most other flax stocks would have a normal non‐male‐sterile cytoplasm [N] and the non‐restorer gene, rf. Male‐sterile plants would be [cms] rf rf and maintainer stocks would be [N] rf rf . Tests using genetic male sterility to identify lines with cytoplasmic restoration of fertility are discussed, together with methods of distinguishing the possible nuclear genotypes. For any species in which most stocks have the nonrestorer [N] type of cytoplasm and the restorer type [c R] is rare, an advantage of this system for producing hybrid seed is the ability to produce large quantities of seed that will give only male‐sterile plants yet not transmit a unique cytoplasm to the hybrid seed offered to farmers. Development and maintenance of the parental stocks can be accomplished by conventional breeding techniques.
A graph-based approach for robust single-shot structured light (2010)

Structured Light is a well-known method for acquiring 3D surface data. Single-shot methods are restricted to the use of only one pattern, but make it possible to measure even moving objects with simple and compact hardware setups. However, they typically operate at lower resolutions and are less robust than multi-shot approaches. This paper presents an algorithm for decoding images of a scene illuminated by a single-shot color stripe pattern. We solve the correspondence problem using a region adjacency graph, which is explicitly designed for robustness against surface color variations. The algorithm runs in real time on input images of 780 × 580 pixels and can generate up to 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">5</sup> data points per frame. Our methodology gives accurate 3D data even under adverse conditions, i.e. for highly textured or volume-scattering objects and low contrast illumination. Experimental results demonstrate the improvement over previous methods.
Oxidation of reduced Cu,Zn superoxide dismutase by molecular oxygen. A kinetic study (1986)

The rate of oxidation of reduced bovine Cu,Zn superoxide dismutase [ECu(I)] by molecular O2 was studied by magnetic-resonance techniques and was found to be low under physiological conditions. The analysis of the kinetic data and of the experiments carried out in the presence of tetranitromethane confirms that O2.- is a product of the oxidation process. At [ECu(I)]/([ECu(I)] + [ECu(II)]) greater than 0.5 the O2.- produced reacts mainly with ECu(I), increasing the oxidation rate of the enzyme, whereas at [ECu(I)]/([ECu(I)] + [ECu(II)]) less than 0.5 it reacts mainly with ECu(II), decreasing the oxidation rate, the kinetics, at constant O2 concentration, being an apparent second-order process. The oxidation rate increased linearly with both O2 and OH- concentration, indicating that only a deprotonated form of the ECu(I) reacts with O2.-.
Flight Profile Performance Handbook. Volume V. YAH-64 (Advanced Attack Helicopter-AAH) (1978)

Abstract : The purpose for preparing this handbook series is fourfold: (a) to validate YAH-64 performance data quickly; (b) to reduce the manpower and time to prepare accurate flight profiles; (c) to standardize performance data so that the analysis community can benefit from a single reference in conducting studies; and (d) to provide a handbook that can be used for training in the mission profile planning area.
Enantioselective Allyltitanations and Metathesis Reactions. Application to the Synthesis of Piperidine Alkaloids (+)-Sedamine and (−)-Prosophylline (2002)

An enantioselective synthesis of the piperidine alkaloids (+)-sedamine and (-)-prosophylline is reported. The synthesis of (+)-sedamine has been achieved in 12 steps with an overall yield of 20% from benzaldehyde, and (-)-prosophylline was obtained in 15 steps with an overall yield of 9.2%, starting from D-glyceraldehyde acetonide 14. The key steps are enantioselective allyltitanation reactions and ring-closing or cross-metathesis reactions.
Addition of Carboxamides to Alkynyl Trifluoromethyl Sulfones (1989)

“Push-pull” compounds 4 are obtained in good yields in an unusual way by addition of carboxamides 2 to the extremely reactive alkynyl trifluoromethyl sulfone 1. A crucial feature of this reaction is the activation of the triple bond in 1 by the F3CSO2 group. Dimethyl sulfoxide also adds spontaneously to 1.
The Lambda time slot permuter: a bit-controlled time slot permuter (1992)

The authors formulated a general method for constructing time slot permuters from spatial permutation networks. Using the method, they constructed a new time slot permuter, the Lambda time-slot permuter. The Lambda time slot permuter is obtained from the bit-controlled, self-routing permutation network, the Lambda network. While the Lambda time slot permuter requires more hardware than the time slot permuter based on the Benes network described by S.V. Ramanan et al. (1990), its control is very simple. In the Lambda time slot permuter, a single bit from the destination tag in the first of a pair of time slots entering an exchange element is used as the control bit so that the switch setting can be done before the second time slot arrives; thus, it is possible that the switch setting does not incur any delay. The Lambda time slot permuter is suitable for a class of applications where on-the-fly control of the time slot permuter is important, and is especially efficient for a pipelined operation. >
Spectral linewidth analysis of semiconductor hybrid lasers with feedback from an external waveguide resonator circuit (2017)

We present a detailed analysis of a semiconductor hybrid laser exploiting spectral control from an external photonic waveguide circuit that provides frequency-selective feedback. Based on a spatially resolved transmission line model (TLM), we have investigated the output power, emission frequency, and the laser spectral linewidth. We find that, if the feedback becomes weaker, the spectral linewidth is larger than predicted by previous models that are based on a modified mean-field approximation, even if these take a strong spatial variation of the gain into account. The observed excess linewidth is caused by additional index fluctuations that are associated with strong spatial gain variations.
ChemInform Abstract: Highly Enantioselective Carbonyl‐ene Reactions Catalyzed by a Hindered Silyl—Salen—Cobalt Complex. (2008)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Asymmetric Induction in the Wittig‐Still Rearrangement of Ethers Containing an Allylic Stereocenter – Diastereocontrol by Allylic Nitrogen (1990)

The stereochemistry of Wittig‐Still rearrangements under the influence of an allylic stereogenic center attached to Bn 2 N or RNBoc groups is studied. Rearrangements are subject to stereocontrol through asymmetric induction. Stannylated allyl ethers 13 with Z double bonds give syn‐configuration amino alcohols 15 exclusively (ds >95 : < 5 ‐ > 99.8 : < 0.2). Stannanes of E configuration 14 lead to 15 less selectively ( syn :anti = 46 : 54 ‐ 92 : 8). X‐ray structural data are provided for amino alcohol syn ‐ 15b and for the bis(carbamate) 17 .