gebhart/patrepeval huggingface dataset:
Parathyroid Hormone-related Protein Promotes Quiescence and Survival of Serum-deprived Chondrocytes by Inhibiting rRNA Synthesis (2001)

Parathyroid hormone-related protein (PTHrP) was initially recognized for its ability to promote parathyroid hormone-like bioactivity in kidney, bone, and squamous epithelial cells. PTHrP is a multifunctional protein in which bioactivity is mediated by two distinct pathways. Its classic parathyroid hormone-like activity results from binding of its amino terminus to cell surface PTH1R and activation of signal transduction pathways. Another less well recognized pathway involves translocation of PTHrP to the nucleus via a mid-region bipartite nuclear targeting sequence (NTS), similar in structure and function to those found in retroviral regulatory proteins. PTHrP was identified in the nucleus of several different cell types in vivo and in vitro, where it has been implicated in cell cycle progression, cellular differentiation, and apoptosis. In previous work we showed that nuclear translocation of PTHrP enhanced the survival of serum-deprived chondrogenic cells, associated with RNA, and localized to a region of the nucleus rich in complexes of newly transcribed ribosomal RNA and protein. In this work we have used two chondrogenic cell lines, CFK2 (PTH1R+) and 27m21 (PTH1R-) to further explore mechanisms whereby PTHrP rescues immature chondrocytes from apoptosis. Endogenous PTHrP and exogenous PTHrP NTS peptide protected serum-deprived cells from apoptosis, in the presence and absence of PTH1R. The survival of cells expressing PTHrP and those treated with PTHrP NTS peptide was associated with a rapid shift into G(o)/G1 accompanied by a significant down-regulation of rRNA synthesis and a decrease in the number of actively translating polyribosome complexes. Together with our previous observations, this work predicts a role for PTHrP in modulating ribosome biogenesis and preventing chondrogenic cells from progressing through the cell cycle in an unfavorable environment.
Discussion on "The organisation of production engineering" at the Luton Section of the Institution (1933)

MR. S. CARLTON-SMITH (Section President): I have great pleasure on behalf of myself and all present in expressing our appreciation and thanks to Mr. Jenkins. It is particularly gratifying to us that we have one of the members of our own section who is an authority to give us this lecture on such an important subject, which is of interest to all members?not only to a certain number of them. It has been a most interesting paper and I am sure we have all enjoyed it.
The Development of a Manufacturable Synthesis of LY213829 (2000)

The development of a manufacturable synthesis of LY213829 (4-thiazolidinone-5-[3,5-bis(1,1-dimethylethyl)-4-hydroxyphenyl] methyl) is described. Rather than reduction to eliminate the thiocarbonyl from the rhodanine moiety, the new route utilizes a novel concurrent ring opening with ammonia and re-cyclization with formaldehyde. This change obviates a potentially problematic zinc solid waste stream.
Rapid detection of methylation change at H19 in human imprinting disorders using methylation-sensitive high-resolution melting (2008)

Beckwith Wiedemann syndrome (BWS) and Russell Silver syndrome (RS) are growth disorders with opposing epimutations affecting the H19/IGF2 imprinting center at 11p15.5. Overgrowth and tumor risk in BWS is caused by aberrant expression of the paternally expressed, imprinted IGF2 gene, occurring as a consequence of mosaic hypermethylation within the imprinting center, or to mosaic paternal uniparental disomy (UPD). RS is characterized by severe intrauterine growth retardation (IUGR). A subset of RS cases were recently shown to have mosaic hypomethylation within the H19/IGF2 imprinting center, predicted to silence paternally expressed IGF2 in early development. Molecular diagnosis for BWS and RS involves methylation analysis of the H19 locus, enabling discrimination of allelic methylation patterns. In this study, methylation-sensitive high-resolution melting analysis (MS-HRM) was used to analyze methylation within the intergenic region of the H19 locus. A total of 36 samples comprising normal control (11), BWS (19), and RS (six) DNA were analyzed in a blinded study and scored as hypermethylated, normal, or hypomethylated. Results were compared with those derived by methylation-sensitive Southern blotting using the restriction enzymes Rsa I and Hpa II. A total of 100% concordance was obtained for the Southern blotting and MS-HRM scores. A total of three samples with paternal duplication affecting the H19/IGF2 region were scored as equivocal by both methods; however, 33 out of 36 (92%) the samples were unambiguously scored as being hypermethylated, hypomethylated, or normally methylated using MS-HRM. We conclude that MS-HRM is a rapid, cost-effective, and sensitive method for screening mosaic methylation changes at the H19 locus in BWS and RS.
Phosphorylation of <i>Aspergillus fumigatus</i> PkaR impacts growth and cell wall integrity through novel mechanisms (2017)

Protein kinase A (PKA) signaling is essential for growth and virulence of the fungal pathogen Aspergillus fumigatus. Little is known concerning the regulation of this pathway in filamentous fungi. Employing liquid chromatography-tandem mass spectroscopy, we identified novel phosphorylation sites on the regulatory subunit PkaR, distinct from those previously identified in mammals and yeasts, and demonstrated the importance of two phosphorylation clusters for hyphal growth and cell wall-stress response. We also identified key differences in the regulation of PKA subcellular localization in A. fumigatus compared with other species. This is the first analysis of the phosphoregulation of a PKA regulatory subunit in a filamentous fungus and uncovers critical mechanistic differences between PKA regulation in filamentous fungi compared with mammals and yeast species, suggesting divergent targeting opportunities.
Hydrolysis of DNA by Ce(IV)/EDTA complexes--The mechanism of DNA cleavage and design of artificial restriction enzymes-- (2000)

Homogeneous Ce(IV) complex of EDTA promptly hydrolyzes oligonucleotides under physiological conditions. Moreover, the activity of Ce(IV)/EDTA for DNA hydrolysis is promoted by the addition of amines. When [Ce(IV)/EDTA] = 5 mumol dm3 and [ethylenediamine] = 100 mmol dm3, the catalytic activity is about 50 times as large as that of Ce(IV)/EDTA. The combination of Ce(IV)/EDTA and amines is eminent tools for the future molecular biology and biotechnology.
The Complementary Roles of Amine Catalysts and Applied Statistics in HR Molded Foam Quality Improvement (1985)

Amelioration de la qualite des sieges d'automobile a haute resilience en polyurethanne grâce a de nouveaux catalyseurs (amine) et d'utilisation de methodes statistiques
Ghost femtocells: A novel radio resource management scheme for OFDMA based networks (2011)

The femtocell deployment in 3GPP/LTE sets new challenges to interference mitigation techniques and Radio Resource Management (RRM). Traditional schemes are mainly designed for classical cellular networks while the ad hoc nature of femtocells notably limits the complexity of possible algorithms. Thus, efficient RRM schemes are essential for limiting the interference impact on end-user performance. The goal of this paper is to achieve effective spectral reuse between macrocells and femtocells while guaranteeing the QoS of users served by both macro and femto base stations. We propose a novel resource management scheme that limits the overall interference per chunk generated outside the coverage range of a femtocell while reducing the transmission power in each Resource Block (RB). Our simulation results show that the proposed RRM scheme enhances the energy efficiency of femtocells and improves both macrocell and femtocell throughput.
Matrix Metalloproteinase Gelatinase B (MMP-9) Coordinates and Effects Epithelial Regeneration (2002)

We studied the role of the matrix metalloproteinase gelatinase B (gelB; MMP-9) in epithelial regeneration using the gelB-deficient mouse. We report the novel finding that, in contrast to other MMPs expressed at the front of the advancing epithelial sheet in wounds of cornea, skin, or trachea, gelB acts to inhibit the rate of wound closure. We determined this to be due to control of cell replication, a novel capacity for MMPs not previously described. We also found that gelB delays the inflammatory response. Acceleration of these processes in gelB-deficient mice is correlated with a delay in signal transduction through Smad2, a transcription factor that inhibits cell proliferation, and in accumulation of epithelial-associated interleukin-1alpha, a cytokine that inhibits Smad2 signaling and promotes the inflammatory response. GelB-deficient mice also reveal defects in remodeling of extracellular matrix at the epithelial basement membrane zone, in particular, failure to effectively remove the fibrin(ogen) provisional matrix. We conclude that gelB coordinates and effects multiple events involved in the process of epithelial regeneration.
Vaccinium ovatum Pursh (1999)

https://thekeep.eiu.edu/herbarium_specimens_byname/16333/thumbnail.jpg
ChemInform Abstract: Synthesis of a Novel Trisubstituted Cyclobutane Nucleoside Analogue. Unexpected C4‐C3 Ring Contractions in Related Reactions. (1997)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
pp60v-src tyrosine kinase is expressed and active in sarcoma-free avian embryos microinjected with Rous sarcoma virus. (1988)

Early embryonic avian tissue is resistant to transformation by Rous sarcoma virus. To determine the nature of this resistance, we examined the expression and properties of the Rous sarcoma virus transforming protein pp60v-src, in infected embryonic chicken limbs in ovo. Lysates from Rous sarcoma virus-infected limbs contained the viral structural protein p19gag, as detected by immunoblot analysis, and showed pp60v-src kinase activity in vitro. Immunoblot analysis of lysates with anti-phosphotyrosine antibodies revealed a number of phosphotyrosine-containing proteins present in lysates of Rous sarcoma virus-infected embryos but not in lysates of control, uninfected embryos. Anti-phosphotyrosine immunoreactivity was observed in frozen sections in the same cell types that expressed pp60v-src and p19gag. These studies demonstrate that pp60v-src is co-expressed with viral structural determinants in infected embryonic avian tissue. Furthermore, pp60v-src is active in ovo as a tyrosine-specific phosphotransferase, despite the apparent lack of sarcoma induction. The localization pattern of the major src gene substrate p36 (calpactin I) was compared with that of p19gag by double-label immunofluorescence and found to be generally nonoverlapping. These observations are consistent with the concept that the induction of tumors in ovo requires complementation between viral determinants and host factors. These host factors, which may be critical substrates of pp60v-src, are subject to developmental regulation in the avian embryo.
Depth Resolved Differences After Corneal Crosslinking With and Without Epithelial Debridement Using Multimodal Imaging (2014)

Purpose: : To quantitatively compare stromal collagen architecture and keratocyte viability between two methods of corneal crosslinking (CXL), one involving invasive epithelial debridement (standard riboflavin crosslinking) and the other by the modified method of riboflavin TRIS–ethylenediaminetetraacetic acid (riboflavin-TE) transepithelial crosslinking, which conserves epithelial layer, using multimodal imaging. Methods: : Corneas of fresh porcine globes were treated with standard or with riboflavin-TE crosslinking for 30 minutes under a 370-nm ultraviolet A (UVA) light source (3 mW/cm2). Depth resolved imaging by confocal and second harmonic generation (SHG) microscopy was performed after live/dead cell labeling. Evaluation of apoptotic keratocyte number and texture analysis based on surface roughness was performed on confocal and SHG image stacks, respectively. Results: : Surface texture and visual grading indicated significant alterations in stromal architecture following standard CXL compared with untreated controls, while riboflavin-TE CXL induced subtle alterations confined near the corneal surface. The standard CXL had a statistically lower (P < 0.05) average surface roughness (9.46 ± 2.8 AU), with no significant difference between controls (12.85 ± 2.96 AU) and riboflavin-TE CXL (14.34 ± 1.85 AU). Both CXL groups resulted in apoptotic keratocytes, differing significantly from controls, with 56.5% greater numbers of apoptotic cells in the standard CXL group than the TE-CXL group (P < 0.05). Conclusion: : Standard CXL produced significant architectural changes of the deeper stroma and caused increased collateral damage on the keratocytes in comparison to the riboflavin-TE CXL at all depths studied. Translational Relevance: : Multimodal imaging guided surface architectural analysis provided insights into the actual collagen changes that occur after CXL and may guide in real-time modifications of the CXL methods for effective therapeutic CXL.
Codes from order domains (2002)

We describe codes coming from order domains of arbitrary transcendence degree. This is a generalization of the improved duals of 1-point geometric Goppa codes. We can construct rather long and relatively good codes.
A Novel Concept for Americium-containing Target for Use in Fast Reactors (2006)

A novel concept for the americium-containing target, which consists of (Th,Am)O2-x and molybdenum support material, is proposed that can fulfill several requirements such as reduction of the environmental burden and effective use of natural resources. In particular, deployment of recovered molybdenum from the spent nuclear fuel is highlighted and its nuclear effects are focused on in comparison to other types of molybdenum, namely natural and enriched ones. A preliminary investigation for effects of molybdenum isotopic composition on fast reactor core characteristics were carried out using nuclear calculation codes. The results indicated the feasibility of the fast reactor core loaded with the present target by optimizing its amount and composition. Moreover, several additional gains were indicated such as possible use of the radioactive waste and production of a new energy source, 233U.
Natural Killer (NK) and T Cell-Associated Surface Marker Expression Following Allogeneic and Autologous Bone Marrow Transplantation (BMT) (2000)

Natural killer (NK) and T cell development was studied after allogeneic and autologous BMT. We determined the phenotypic expression and lytic ability of these subpopulations after BMT. Following T cell-depleted (TCD) BMT, the number of CD16+ and CD56+ cells peaked at 39 and 46 days, respectively, and constituted the majority of peripheral blood lymphocytes (PBL). Coexpression of CD3 and CD16 was <10% up to 14.5 weeks after transplant. Following allogeneic non-T cell-depleted (NTCD) BMT, the number of CD16+ and CD56+ cells peaked at 6 weeks. CD3 expression was normal (70%-80%), % CD8+ cells was high (40%), and % CD4+ cells was low (20%). Following autologous BMT (ABMT), % CD3+ T cells was 80%, of which 70% expressed the CD8 marker. In contrast, CD4 expression was low (20%). CD16+ cells appeared 2.5-3 weeks after ABMT but with low frequency (20%), at which point 20%-30% of the CD3+ cells coexpressed CD16. A positive correlation was found between CD16 expression and cytotoxic capability. In conclusion, a marked difference was observed in NK and T cell-associated markers following TCD BMT, NTCD BMT, and ABMT. Following NTCD or ABMT, but not TCD BMT, a high percentage of cells co-express CD16 and CD3, which may indicate the possibility of a common NK and T cell progenitor.
Proceedings: The EEG effects of VUFB-10.032, clozapine and octoclothepin in rabbits. (1975)

<P>Abstract copyright UK Data Service and data collection copyright owner.</P>
CO-CURING OF EPDM AND NR (II) (1976)

エラストマーブレンドにおける共加硫の問題は, 今まで数多く研究され, 最近では異相界面共架橋の問題が注目されている. この問題を解決するために, EPDM/NRブレンドにおいて架橋点間距難の測定と電子顕微鏡写真から解析していった. その結果, ブレンド物におけるEPDM相の架橋は加硫時間とともに増加するが, 両相との異相界面共架橋が形成されないために共加硫していないことがわかった. 一方, 酸北亜鉛のない系で硫黄と加硫促進剤とをあらかじめ反応させたところのペンダントサルファーEPDM (P-EPDM) とNRとのブレンドにおいては, P-EPDM相の架橋度はEPDM相よりも改善されているが, まだ充分ではない. しかし, 異相界面共架橋が形成されるために, 共加硫できることがわかった.
Rocket Gamma Scanning of Nuclear Bursts (1966)

A rocketborne instrument for locating and mapping the gamma radiating debris cloud produced by high-altitude nuclear explosions has been developed and tested. The instrument contains two scintillating type detectors assembled into a directional uranium shield. Two detectors are employed in order to optimize sensitivity and angular resolution over the large dynamic range of gamma flux rates experienced during the lifetime of the debris cloud. A raster of celestial space is developed by slowly scanning the directional detectors in elevation while the payload rotates in azimuth.
Tryptophan pyrrolase in haem regulation. Experiments with administered haematin and the relationship between the haem saturation of tryptophan pyrrolase and the activity of 5-aminolaevulinate synthase in rat liver (1980)

1. Administration of haematin to rats decreases 5-aminolaevulinate synthase activity in whole liver homogenates. 2. An inverse relationship between this decrease and the increase in saturation of apo-(tryptophan pyrrolase) with haem is observed during the initial phase of treatment with haematin. 3. Significant changes in both functions are caused by a 1 mg/kg dose of haematin, whereas the maximum effects are achieved by the 5 mg/kg dose. 4. Prevention by allopurinol of the conjugation of exogenously administered haematin with apo-(tryptophan pyrrolase) renders this haem available for further repression of 5 aminolaevulinate synthase. 5. The various aspects of the relationship between synthase activity and the haem saturation of tryptophan pyrrolase are discussed.
Comparison of the Sintering Behavior of SHS and ARC Melted Ti5Si3 (2008)

Single phase Ti5Si3 was synthesized by SHS and arc-melting of elemental powders. The synthesized powders were ground and classified to < 20 μm. After sintering at 1800°C or 1900°C, in an argon atmosphere, the densities were found to be similar for both SHS and arc-melted materials. However, when the form of the reactants for arc-melting were changed from powders to solid lumps, lower sintered densities were obtained. The difference in sintered densities was attributed to the oxygen content. To support this hypothesis, Ti5Si3 was intentionally doped with oxygen and sintered under identical conditions. Ti5Si3 doped with oxygen had the highest sintered density among all the samples investigated.
The sensitivity and specificity of monoclonal antibodies URO-2 and 19-9 in the immunohistochemical classification of primary adenocarcinomas (1986)

The specificity and sensitivity of commercially available monoclonal antibodies (McAb) to tumor antigens were tested using McAb 19-9, derived from a colorectal adenocarcinoma, and URO- 2, derived from a renal adenocarcinoma. Using the avidin-biotincomplex technique and frozen tissue, 53 adenocarcinomas representing lung, breast, colorectal, ovarian, renal, and uterine origin were studied. All slides were randomized and subsequently evaluated for staining intensity and percent tumor-area positivity. The sensitivity and specificity of 19-9 for colorectal adenocarcinoma were 74% and 54%, respectively. The sensitivity and specificity of URO-2 for renal adenocarcinoma were 100% and 87.5%, respectively. In most cases, URO-2 stained greater than 75% of the renal tumor area; however, 19-9 stained less than 50% of the colorectal tumor area.
Au<sub>137</sub>(SR)<sub>56</sub>nanomolecules: composition, optical spectroscopy, electrochemistry and electrocatalytic reduction of CO<sub>2</sub> (2014)

Au137(SR)56, a nanomolecule with a precise number of metal atoms and ligands, was synthesized. The composition was confirmed by MALDI and ESI mass spectrometry using three unique ligands (–SCH2CH2Ph, –SC6H13, and –SC4H9) and nano-alloys with Ag and Pd. The electrocatalytic properties were tested for CO2 reduction.
ChemInform Abstract: Reactions of Molybdenum Oxo‐complexes with Substituted Hydrazines. Part 1. The Preparation of Molybdenum Alkyl‐ and Aryl‐imido‐complexes; the Crystal and Molecular Structure of (N‐Benzoyl‐N′‐p‐tolyldiazene‐N′O)dichloro(dimethylphenyl‐phosphine) (p‐tolylimido)molybdenum. (1979)

Abstract Die Oxo‐Mo(IV)‐Komplexe (I) reagieren mit substituierten Hydrazinen (II) unter Rückfluß in MeOH zu den Imido‐Komplexen (III), für die in den meisten Fällen keine Ausb. angegeben ist.
Novel protein vaccine candidates against Group B streptococcal infection identified using alkaline phosphatase fusions (2003)

Using an alkaline phosphatase-based genetic screening method, we identified a number of proteins that are potentially located on the outer surface of Group B streptococcus (Streptococcus agalactiae). In an enzyme-linked immunosorbent assay, antisera raised against two of the proteins, the streptococcal yutD homologue and a subunit of an ABC transporter, recognised clinically important serotypes of Group B streptococcus. In a neonatal rat model, purified IgG from the sera conferred significant levels of protection against a lethal challenge infection. The proteins identified show potential as protein subunit candidates for vaccines against Group B streptococcal disease in neonates.
Epidemiology of Fatal Occupational Injuries in Jefferson County, Alabama (2002)

High-risk industries should consider worker-targeted injury prevention programs, and particular attention should be paid to elderly workers.
Polarographic Study on Interaction between Human IgG and Sheep Antihuman IgG Antiserum and Its Analytical Application (1983)

Journal Article Polarographic Study on Interaction between Human IgG and Sheep Antihuman IgG Antiserum and Its Analytical Application Get access Kenji Kano, Kenji Kano Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University, Kyoto 606, Japan Present address: Gifu College of Pharmacy, Mitahora-higashi 5–6–1; Gifu 502, Japan. Search for other works by this author on: Oxford Academic Google Scholar Tokuji Ikeda, Tokuji Ikeda Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University, Kyoto 606, Japan Search for other works by this author on: Oxford Academic Google Scholar Mitsugi Senda Mitsugi Senda Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University, Kyoto 606, Japan Search for other works by this author on: Oxford Academic Google Scholar Agricultural and Biological Chemistry, Volume 47, Issue 5, 1 May 1983, Pages 1043–1047, https://doi.org/10.1080/00021369.1983.10865753 Published: 01 May 1983 Article history Received: 27 October 1982 Published: 01 May 1983