gebhart/patrepeval huggingface dataset:
Easy method for newborn screening of six lysosomal storage disorders using online solid-phase extraction with mass spectrometry / Altı lizozomal depo hastağının yenidoğan taramasında, online katı faz eksraksiyon kullanılarak kütle spektrometresi ile ölçümünde kolay bir metod (2016)

Abstract Objective: A modified method for screening of six lysosomal storage disorders (LSDs) by tandem mass spectrometry was presented. Methods: The enzyme activities for six LSDs (Gaucher, Pompe, Krabbe, Fabry, Niemann-Pick A/B and Mucopolysaccharidosis Type I) was measured by using ultra- HPLC and mass spectrometry. After overnight incubation of dried blood spots with three distinct reaction cocktails containing substrates and internal standards, reactions were stopped and online trapping was performed with ultra-HPLC preceding to mass spectrometry. Ultra-HPLC was equipped with online solid phase extraction and Hypersil Gold C8 analytical columns and coupled with TSQ Quantum Access Max mass spectrometry. Results: Activities of acid-s-glucocerebrosidase (ABG), acid glucosidase (GAA), galactocerebroside-s-galactosidase (GALC), acid-galactosidase A (GLA), acid sphingomyelinase (ASM), and α-L-iduronidase (IDU) were obtained from DBSs of patients and healthy individuals. The intraand inter-assay precisions were <20% (CV). Conclusion: Our modified method, needing less DBS punches and only three reaction coctails, with the online trapping methodology, accurately differentiates newborns with LSDs from healthy newborns.
System design methodologies for a wireless security processing platform (2002)

Security protocols are critical to enabling the growth of a wide range of wireless data services and applications. However, they impose a high computational burden that is mismatched with the modest processing capabilities and battery resources available on wireless clients. Bridging the security processing gap, while retaining sufficient programmability in order to support a wide range of current and future security protocol standards, requires the use of novel system architectures and design methodologies.We present the system-level design methodology used to design a programmable security processor platform for next-generation wireless handsets. The platform architecture is based on (i) a configurable and extensible processor that is customized for efficient domain-specific processing, and (ii) layered software libraries implementing cryptographic algorithms that are optimized to the hardware platform. Our system-level design methodology enables the efficient co design of optimal cryptographic algorithms and an optimized system architecture. It includes novel techniques for algorithmic exploration and tuning, performance characterization and macro-modeling of software libraries, and architecture refinement based on selection of instruction extensions to accelerate performance-critical, computation-intensive operations. We have designed a programmable security processor platform to support both public-key and private key operations using the proposed methodology, and have evaluated its performance through extensive system simulations as well as hardware prototyping. Our experiments demonstrate large performance improvements (e.g., 31.0X for DES, 33.9X for 3DES, 17.4X for AES, and upto 66.4X for RSA) compared to well-optimized software implementations on a state-of-the-art embedded processor.
Serum interleukin-6 as a prognostic biomarker in patients with metastatic melanoma (2012)

Interleukin-6 (IL-6) is an immunomodulatory cytokine produced by both normal cells and tumor cells, including melanoma cells. The specific biological function of IL-6 in melanoma is unknown. The present study examined whether the serum concentration of IL-6 can predict prognosis in patients with metastatic melanoma. IL-6 was measured by ELISA in serum samples from 103 patients with metastatic melanoma obtained before IL-2-based immunotherapy. Patients with metastatic melanoma had higher serum IL-6 than healthy individuals (median 3.4 ng/l, range 0.3-93 ng/l vs. median 1.4 ng/l, range 0.25-22.5 ng/l, P<0.0001). Pretreatment serum IL-6 was elevated in 43% of the patients. Patients with elevated pretreatment serum IL-6 had shorter overall survival (OS) compared with patients with normal serum IL-6 (P<0.0002). The median OS was 10.8 months [95% confidence interval (CI): 8.86-13.46] in patients with normal serum IL-6 compared with 4.5 months (95% CI: 3.04-7.39) in patients with elevated serum IL-6. Multivariate Cox analysis showed that serum IL-6 [hazard ratio (HR)=1.82, 95% CI: 1.19-2.78, P=0.006] and serum lactate dehydrogenase (HR=2.02, 95% CI: 1.31-3.11, P=0.001) were independent prognostic biomarkers of OS. A combination variable of elevated serum IL-6 and elevated serum lactate dehydrogenase almost quadrupled the risk of early death (HR=3.67, 95% CI: 2.17-6.20, P<0.0001) compared with patients with normal serum levels of these two biomarkers. Elevated serum IL-6 is an independent prognostic biomarker of short OS in patients with metastatic melanoma. A larger retrospective study is ongoing to confirm the findings. To validate serum IL-6 further as a prognostic biomarker, a prospective study is required.
Hypnotic drug risks of mortality, infection, depression, and cancer: but lack of benefit (version 1; referees: 2 approved) (2016)

This is a review of hypnotic drug risks and benefits, reassessing and updating advice presented to the Commissioner of the Food and Drug Administration (United States FDA). Almost every month, new information appears about the risks of hypnotics (sleeping pills). This review includes new information on the growing USA overdose epidemic, eight new epidemiologic studies of hypnotics' mortality not available for previous compilations, and new emphasis on risks of short-term hypnotic prescription. The most important risks of hypnotics include excess mortality, especially overdose deaths, quiet deaths at night, infections, cancer, depression and suicide, automobile crashes, falls, and other accidents, and hypnotic-withdrawal insomnia. The short-term use of one-two prescriptions is associated with greater risk per dose than long-term use. Hypnotics are usually prescribed without approved indication, most often with specific contraindications, but even when indicated, there is little or no benefit. The recommended doses objectively increase sleep little if at all, daytime performance is often made worse, not better, and the lack of general health benefits is commonly misrepresented in advertising. Treatments such as the cognitive behavioral treatment of insomnia and bright light treatment of circadian rhythm disorders might offer safer and more effective alternative approaches to insomnia.
Ether-tethered Ru(ii)/TsDPEN complexes; synthesis and applications to asymmetric transfer hydrogenation (2011)

A new type of Ru(II)/TsDPEN catalyst containing an ether-based linking tether has been prepared and shown to exhibit excellent activity in asymmetric transfer hydrogenation reactions of ketones. Related complexes containing a hydroxyl-terminated alkyl chain have also been prepared and tested as asymmetric catalysts. In some cases the activity of the new catalyst type complements that of the closely related alkyl-chain tethered complexes.
Mutant Rac1B expression in Dictyostelium: Effects on morphology, growth, endocytosis, development, and the actin cytoskeleton (2000)

Rac1 is a small G-protein in the Ras superfamily that has been implicated in the control of cell growth, adhesion, and the actin-based cytoskeleton. To investigate the role of Rac1 during motile processes, we have established Dictyostelium cell lines that conditionally overexpress epitope-tagged Dictyostelium discoideum wild-type Rac1B (DdRac1B) or a mutant DdRac1B protein. Expression of endogenous levels of myc-or GFP-tagged wild-type DdRac1B had minimal effect on cellular morphologies and behaviors. By contrast, expression of a constitutively active mutant (G123 V or Q613 L) or a dominant negative mutant (T173 N) generated amoebae with characteristic cellular defects. The morphological appearance of actin-containing structures, intracellular levels of F-actin, and cellular responses to chemoattractant closely paralleled the amount of active DdRac1B, indicating a role in upregulating actin cytoskeletal activities. Expression of any of the three mutants inhibited cell growth and cytokinesis, and delayed multicellular development, suggesting that DdRac1B plays important regulatory role(s) during these processes. No significant effects were observed on binding or internalization of latex beads in suspension or on intracellular membrane trafficking. Cells expressing DdRac1B-G12V exhibited defects in fluid-phase endocytosis and the longest developmental delays; DdRac1B-Q61L produced the strongest cytokinesis defect; and DdRac1B-T17N generated intermediate phenotypes. These conditionally expressed DdRac1B proteins should facilitate the identification and characterization of the Rac1 signaling pathway in an organism that is amenable to both biochemical and molecular genetic manipulations. Cell Motil.
Phenome-Wide Association Study (PheWAS) for Detection of Pleiotropy within the Population Architecture using Genomics and Epidemiology (PAGE) Network (2013)

Using a phenome-wide association study (PheWAS) approach, we comprehensively tested genetic variants for association with phenotypes available for 70,061 study participants in the Population Architecture using Genomics and Epidemiology (PAGE) network. Our aim was to better characterize the genetic architecture of complex traits and identify novel pleiotropic relationships. This PheWAS drew on five population-based studies representing four major racial/ethnic groups (European Americans (EA), African Americans (AA), Hispanics/Mexican-Americans, and Asian/Pacific Islanders) in PAGE, each site with measurements for multiple traits, associated laboratory measures, and intermediate biomarkers. A total of 83 single nucleotide polymorphisms (SNPs) identified by genome-wide association studies (GWAS) were genotyped across two or more PAGE study sites. Comprehensive tests of association, stratified by race/ethnicity, were performed, encompassing 4,706 phenotypes mapped to 105 phenotype-classes, and association results were compared across study sites. A total of 111 PheWAS results had significant associations for two or more PAGE study sites with consistent direction of effect with a significance threshold of p<0.01 for the same racial/ethnic group, SNP, and phenotype-class. Among results identified for SNPs previously associated with phenotypes such as lipid traits, type 2 diabetes, and body mass index, 52 replicated previously published genotype-phenotype associations, 26 represented phenotypes closely related to previously known genotype-phenotype associations, and 33 represented potentially novel genotype-phenotype associations with pleiotropic effects. The majority of the potentially novel results were for single PheWAS phenotype-classes, for example, for CDKN2A/B rs1333049 (previously associated with type 2 diabetes in EA) a PheWAS association was identified for hemoglobin levels in AA. Of note, however, GALNT2 rs2144300 (previously associated with high-density lipoprotein cholesterol levels in EA) had multiple potentially novel PheWAS associations, with hypertension related phenotypes in AA and with serum calcium levels and coronary artery disease phenotypes in EA. PheWAS identifies associations for hypothesis generation and exploration of the genetic architecture of complex traits.
Inhibition of Ultraviolet-Induced Chromosome Breaks by Cysteamine in 5-Bromouracil-Substituted Mammalian Cells (1967)

It has been demonstrated that the presence of cysteamine during irradiation brings about the reduction of UV-induced chromosome breaks in BU-substituted mammalian cells. Cysteamine had no or considerably less effect on the UV-induced chromosome breaks in non-BU-substituted cells, nor did it enhance the resumption of UV-induced inhibition of DNA synthesis in BU-substituted cells. The results have been interpreted to indicate that BUdR sensitization of UV-induced chromosomal damage in mammalian cells is due to the nature of the BU photoproduct and not to BU photoproduct inhibition of a rejoining process. Also, UV-induced photoproducts in BU-substituted cells that cause chromosome breaks might not be those that cause UV inhibition of DNA synthesis.
Noncontiguous finished genome sequence and description of Weeksella massiliensis sp. nov. (2015)

Strain FF8(T) (= CSUR P860 = DSM 28259) was isolated in Dakar, Senegal, from the urine of a 65-year-old man with acute cystitis. This strain shows a similarity of sequence of 16S rRNA of 98.38% with Weeksella virosa, and its GenBank accession numbers are HG931340 and CCMH00000000. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis had a poor score, ranging from 1.32 to 1.56, that did not allow identification of the bacterium. Using a polyphasic study made of phenotypic and genomic analyses, strain FF8(T) was a Gram-negative, aerobic rod and a member of the family Flavobacteriaceae. The sequenced genome is 2 562 781 bp with one chromosome but no plasmid. It exhibits a G + C content of 35.9% and contains 2390 protein-coding and 56 RNA genes, including a complete rRNA operon. On the basis of these data, we propose the creation of Weeksella massiliensis sp. nov.
Ultrasound for spinal anesthesia (2015)

The use of ultrasound to aid pediatric spinal anesthesia is a relatively recent advancement. Ultrasound imaging of the neuraxis in children (especially in infants <6 months of age) is a simpler task than in adults, as a result of a largely cartilaginous posterior vertebral column. It is still considered an advanced imaging technique due to the limited ultrasound window. Absence of the ossification associated with ageing greatly increases penetration by the ultrasound beam, thereby producing a clearer image of neuraxial structures. Structures not normally seen in scans of the adult neuraxis (spinal cord, conus medullaris, cauda equina, vertebral body) and even the spread of local anesthetic (LA) in the epidural space may be visualized in infants (Chawathe et al., 2003). Although ultrasound images of neuraxial structures are more limited with increasing age due to ossification of posterior elements, it can still be useful in identifying structures of interest in older children (Marhofer et al., 2005).
Multi-point data over GSM Phase 2 (1991)

Outlines a proposal for general purpose enhanced multi-point data services for GSM. Two services are proposed which are being discussed currently with the GSM. Firstly, a downlink to distribute information to mobile stations (MSs) at a data rate of up to 9.6 kbit/s and secondly, an uplink to collect, efficiently, short status messages from vehicles. All the multi-point services terminate at a service centre and are connected to the terrestrial end of a public land mobile network (PLMN). Although of a general nature, the new services would fulfil the requirements of a comprehensive road traffic informatics (RTI) system based on GSM mobile communications. >
Sterol and fatty acid composition of neutral lipids of<i>Paratenuisentis ambiguus</i> and its host eel (1994)

The sterol composition of free sterol and steryl ester fractions of the fish parasite Paratenuisentis ambiguus was determined. In addition, the fatty acid composition of various neutral lipid classes, i.e., wax esters, steryl esters, triacylglycerols and free fatty acids, as well as the composition of the 1-O-alkyl moieties of total ether glycerolipids of the parasite, were investigated. The results of these studies were compared with those obtained on the intestinal tract tissue of its host, the eel (Anguilla anguilla). Cholesterol is the major sterol in both P. ambiguus and A. anguilla. However, the sterols of P. ambiguus contain high proportions (> 20%) of other sterols, such as campesterol and various dehydrosterols. [e.g., 7-dehydrocholesterol and cholesta-5,22(E)-dienol]. The presence of these minor sterols agrees with the known biotransformations of exogenous sterols in various helminths. Considerable differences are found in the fatty acid composition of neutral lipid fractions, as well as the total lipid extract from the endoparasite as compared to the host tissue. In particular, eicosapentaenoic acid (20:5n-3), other polyunsaturated fatty acids, such as 20:4n-6, 22:5n-3 and 22:6n-3, as well as long-chain saturated fatty acids, such as 20:0, are generally enriched in the neutral lipid fractions of the parasite as compared to those of infected eel intestine.(ABSTRACT TRUNCATED AT 250 WORDS)
Isolation and Characterization of TGF-β2 and TGF-β5 from Medium Conditioned by Xenopus XTC Cells (1990)

TGF-beta 2 and -beta 5 have been purified from medium conditioned by Xenopus cultured cells (XTC) and identified based on their N-terminal amino acid sequence analysis and biological activity. When applied in high concentrations, Xenopus TGF-beta 2, like porcine TGF-beta 2, induces expression of mesodermal markers from cultured Xenopus ectodermal explants, whereas TGF-beta 5 is inactive in this assay. However, the TGF-beta 's could be separated from the major mesoderm-inducing activity present in XTC medium. Xenopus TGF-beta 2 and -beta 5 are approximately equivalent to TGF-beta 1 in their abilities to inhibit the growth of mink lung CCL-64 cells, induce anchorage-independent growth of rat NRK cells, inhibit the proliferation and antibody secretion of human B-lymphocytes, and stimulate chemotaxis of human monocytes. These data establish the functional activity of TGF-beta 5 and suggest that more complex multicellular systems, in contrast to most isolated cells, discriminate between the different TGF-beta s.
Procedimiento de obtención de compuestos órgano-minerales derivados de sílices y de silicatos por tratamiento con isocianatos (1979)

Referencia OEPM: P0479518.-- Fecha de solicitud: 10/04/1979.-- Titular: Consejo Superior de Investigaciones Científicas (CSIC).
Effect of aging on the secondary esophageal peristalsis: presbyesophagus revisited (1995)

In this study we determined the effect of aging on the capability of the human esophagus to generate secondary peristalsis. We studied nine healthy young (35 +/- 2 yr, 25-45 yr) and nine healthy elderly (74 +/- 3 yr, 70-83 yr) volunteers. We stimulated secondary peristalsis by intraesophageal air injection and balloon distension. All young volunteers exhibited secondary esophageal peristalsis. In four elderly volunteers, secondary peristalsis could not be elicited with injection of any of the tested air volumes. Frequency of stimulation of secondary peristalsis and lower esophageal sphincter (LES) relaxation in response to intraesophageal air distension in the elderly was significantly lower than that in the young (P < 0.01). Stimulation of secondary peristalsis by balloon distension was less consistent compared with the air injection. In conclusion, 1) in the elderly, compared with the young, secondary esophageal peristalsis is either absent or is evoked less frequently after esophageal distension, and complete LES relaxation in response to esophageal air distension is less frequent, and 2) in both young and elderly, secondary esophageal peristalsis is induced more frequently after generalized esophageal distension by air than its segmental distension by a balloon.
Abstract 16386: Genome-Wide Association Study of Native Platelet Function in African Americans (2011)

Background: We have previously shown that platelet aggregation, an essential component of pathogenesis of acute coronary syndromes, has higher heritability in African Americans than Caucasians. How...
Posterior Cerebral Hypoperfusion in Migraine Without Aura (2008)

In cerebral blood flow studies, migraine aura is characterized by a posterior cortical hypoperfusion. In contrast, only rare and mild changes in brain perfusion have been demonstrated in migraine without aura, suggesting two different haemodynamic patterns in migraine with and without aura. Our aim was to study hypoperfusion with positron emission tomography (PET) as early as possible during spontaneous migraine without aura attacks. We used H(2) (15)O PET to investigate seven patients (six female, one male) with migraine without aura (International Classification of Headache Diseases-II code 1.1) in three situations: during the headache phase, after headache relief following sumatriptan injection, and during an attack-free interval. Statistical analysis was performed with SPM2. Within 4 h after the attack onset, significant relative bilateral posterior cortical hypoperfusion was found and persisted after headache relief following sumatriptan injection. A posterior cortical hypoperfusion demonstrated in migraine without aura could suggest a common pathogenesis in migraine with and without aura. The significance of relative posterior hypoperfusion in migraine without aura is discussed according to the current knowledge of migraine pathogenesis.
Coupled-cavity resonant passive mode-locked Nd:yttrium lithium fluoride laser (1991)

We report coupled-cavity resonant passive mode locking of a Nd:YLF laser. This technique has produced 4-ps pulses at a wavelength of 1.047 microm with 390-mW average power at a 250-MHz repetition rate, corresponding to a 1.6-nJ pulse energy. The Nd:YLF rod was pumped with 1.5 W of power at 798 nm from a Ti:sapphire laser. The nonlinear reflector used in the coupled cavity was an InGaAs/GaAs strained layer multiple-quantum-well sample.
Thin Type Video Tape for Quadraplex VTR (1972)

高密度化のため磁性層厚, ベース厚ともに2/3にした放送用ビデオテープを試作し, 機械的・電気的特性を検討した.機械的強度の減少は, 記録特性, 走行性に対して悪影響がないことが確認された.磁性層厚が薄くなったための録音特性の変化は, 磁場配向角度を30°から40～50°にすることによって, 現用テープとほぼ同等の特性にすることができた.これによって現在の4ヘッドVTRに対して実用性が充分あることがわかった.
Anomalous Copper Results with Use of Porcelain Crucibles (1958)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAnomalous Copper Results with Use of Porcelain CruciblesHarry. Zeitlin, M. M. Frodyma, and George. IkedaCite this: Anal. Chem. 1958, 30, 7, 1284–1286Publication Date (Print):July 1, 1958Publication History Published online1 May 2002Published inissue 1 July 1958https://pubs.acs.org/doi/10.1021/ac60139a027https://doi.org/10.1021/ac60139a027research-articleACS PublicationsRequest reuse permissionsArticle Views16Altmetric-Citations3LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
A proposal of 3D frequency dependent ADI-FDTD with conductive loss (2004)

An electrically frequency dependent three dimensional alternative direction implicit FDTD method which deals with material dispersion based on the Debye model is proposed, taking the conductive loss into account. Numerical formulations are presented in detail for the practical implementation.
Asymmetric Aldol Reaction Induced by Chiral Auxiliary (2006)

An asymmetric aldol reaction based on the use of (4R,5S)-1,5-dimethyl-4-phenylimidazolidin-2-one as a chiral auxiliary is described involving a three-step procedure: preparation of the chiral auxiliary from readily available (–)-ephedrine and urea in a solventless fashion, coupling of the chiral auxiliary to the ester functional group, and aldol condensation via in situ formation of the corresponding boron enolate.