gebhart/patrepeval huggingface dataset:
Fast, convenient, and effective method to transiently transfect primary hippocampal neurons (1999)

Transfection of primary neurons in culture has proven to be experimentally challenging in the past. To overcome these limitations, we present a detailed transfection protocol for hippocampal neurons based on DNA/Ca2+-phosphate coprecipitation. The main advantages being (1) the speed and convenience, (2) the remarkable efficiency of transfection for mature neurons, and (3) consistent health of the neurons upon transfection allowing subsequent manipulations. The strength of this protocol is convincingly demonstrated by the fact that the expressed protein can be detected biochemically on Western blots. J. Neurosci. Res. 58:831–835, 1999. © 1999 Wiley-Liss, Inc.
Imbalance between CD56+bright and CD56+dim natural killer cell subsets in the liver of patients with recurrent hepatitis C after liver transplantation (2014)

Progressive liver fibrosis remains a major problem for patients with recurrent chronic hepatitis C(CHC) after liver transplantation (LT). However, the involvement of natural killer (NK) and natural killer T (NKT) cells, which predominate in the liver, in recurrent CHC after LT remains unclear.In the present study, we investigated the status of NK and NKT cells in the liver and peripheral blood obtained from 10 patients with recurrent CHC after LT (LT-C), 15 patients with CHC, and 7 normal donors for living donor LT. CD56+ NK cells were separated into two subsets: CD56+bright subset, which is identified as major NK cytokine producer, and CD56+dim subset, which has greater spontaneous cytotoxicity. We found a significant decrease in the CD56+bright subset in the liver of patients with LT-C compared to patients with CHC (P<0.01) and normal donors (P=0.03). The expression of inhibitory NK cell receptor NKG2A was significantly increased on intrahepatic CD56+bright subset in LT-C patients, and activated CD69+CD56+dim NK cell subset was significantly increased in the liver of LT-C patients. Our results suggest that a significant imbalance between CD56+bright and CD56+dim NK cell subsets in the liver may contribute to the progression of recurrent CHC after LT.
A New Method for the Preparation of Monoporous Hollow Microspheres (2010)

The feasibility of producing a hollow microsphere with a single hole in its shell by coaxial electrohydrodynamic atomization (CEHDA) is demonstrated. Polymethylsilsesquioxane (PMSQ) was used as a model shell material encapsulating a core of a volatile liquid, perfluorohexane (PFH), which was subsequently evaporated to produce the hollow microspheres. The diameters of the microspheres and of the single surface pore were controlled by varying the flow rate of the components, the concentration of the PMSQ solution, and the applied voltage in the CEHDA process. The particles were characterized by scanning electron microscopy, and the ranges obtained were 275−860 nm for the microsphere diameter and 35−135 nm for the pore size. The process overcomes several of the key problems associated with existing methods of monoporous microsphere formation including removing the need for elevated temperatures, multiple processing steps, and the use of surfactants and other additives.
Real-Time Condition Monitoring of the Electrolytic Capacitors for Power Electronics Applications (2007)

The objective of this paper is to propose a new low cost method to detect the changes in equivalent series resistor (ESR) and the capacitance value of an electrolytic capacitor in order to realize the real-time condition monitoring of an electrolytic capacitor. Experimental and simulation results are discussed to illustrate the proposed condition monitoring technique. In addition, it is shown that the proposed method can be used for a non-stationary system where waveforms are continuously varying in amplitude, frequency, and phase. The proposed on-line failure prediction method has the merits of low cost and circuit simplicity.
The effects of hyperlipidaemia on graft and patient outcome in renal transplantation (2004)

Hyperlipidaemia is a frequent complication after renal transplantation. As to whether total cholesterol (TC) and triglyceride levels are risk factors for cardiovascular disease and graft survival is controversial. The prevalence of hypercholesterolaemia in the transplanted population in Spain has increased over the years, going from 38.8% in 1990 to 48% in 1998. In contrast, the prevalence of hypertriglyceridaemia being approximately 20%, has not shown any significant variation. Transplant recipients with high cholesterol were characterized by increased age, lower proportion of males, higher mean body mass index, lower proportion of HCV antibodies, reduced time on dialysis and diabetes. Patients with high cholesterol were more frequently treated with cyclosporine + MMF + prednisone and less frequently treated with tacrolimus + MMF + prednisone. Hypertriglyceridaemia was more frequent in patients treated with cyclosporine + MMF + prednisone, and these patients showed significantly higher creatinine plasma levels at 1 year and were more frequently treated with lipid-lowering agents. Hypertriglyceridaemia at 3 months after transplantation is associated with worse graft survival (RR 1.078; CI 1.07-1.143; P = 0.011) and greater cardiovascular mortality (RR 1.265; CI 1.20-1.428; P = 0.0002), while treatment with statins has a protective effect on the graft survival (RR 0.64; CI 0.512-0.888; P = 0.0051). In conclusion, in the renal transplant population in Spain, hypertriglyceridaemia rather than hypercholesterolaemia, may exert a deleterious effect on graft and patient survival.
Laser multiphoton dissociation of alkyl cations: 2. Structure-specific fragmentation patterns of isomeric alkyl cations produced from their iodides (1985)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTLaser multiphoton dissociation of alkyl cations: 2. Structure-specific fragmentation patterns of isomeric alkyl cations produced from their iodidesH. Kuehlewind, H. J. Neusser, and E. W. SchlagCite this: J. Phys. Chem. 1985, 89, 26, 5600–5606Publication Date (Print):December 1, 1985Publication History Published online1 May 2002Published inissue 1 December 1985https://pubs.acs.org/doi/10.1021/j100272a008https://doi.org/10.1021/j100272a008research-articleACS PublicationsRequest reuse permissionsArticle Views27Altmetric-Citations21LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
The use of spiral plating and microscopic colony counting for the rapid quantitation of Mycobacterium paratuberculosis (2003)

The technique could greatly hasten the quantitation of viable M. paratuberculosis.
Dietary influences on porcine postweaning diarrhoea (1987)

The occurrence of diarrhoea in piglets within three to 10 days of weaning (postweaning diarrhoea; PWD) has long been associated with the proliferation of certain serotypes of beta-haemolytic Escherichia coli in the proximal small intestine of affected animals (Richards and Fraser, 1961). Many of these E. coli isolates have been found to be enterotoxigenic [ETEC], producing either heat stable toxin 'a' [STa], heat stable toxin 'b' [STb], heat labile toxin [LT], or combinations of these; some strains also produce shiga-like vero cell cytotoxins [VT] (Smith et al., 1983; Morris and Sojka, 1985). Of these toxins, LT and STb cause fluid accumulation in intestinal loops of weaned pigs, but STa does not (Burgess et al., 1978). Forms of VT may be involved in the production of oedema disease in weaned pigs (Dobrescu, 1983; Smith et al., 1983), but their role in PWD is uncertain. Many ETEC from PWD lack the well-characterized adhesins found on E. coli from neonatal diarrhoea (i.e. K88, K99, 987P or F41), but may possess other uncharacterized adhesins (Nakazawa et al., 1987; Okerman, 1987). The haemolysin of E. coli isolates from PWD does not appear to play a part in the aetiology of the diarrhoea (Smith and Linggood, 1971), and non- haemolytic ETEC may on occasions also be recovered from natural cases of PWD (Hoblet et al., 1986). Initial infection with PWD-producing strains ofETEC can occur in the farrowing house, the organisms then being carried into the weaner house undetected in the intestinal tract (Miller et al., 1984a); alternatively the ETEC infecting weaned pigs can originate in the contaminated environment of the weaner house (Hampson et al., 1987).\n\nDespite the strong association between infection with haemolytic ETEC and occurrence of PWD, oral dosing of weaned pigs with these bacteria does not invariably reproduce the condition (Smith and Jones, 1963; Kenworthy and Allen, 1966; Armstrong and Cline, 1977). The organisms can also be found in the intestinal tract of healthy litter mates of pigs with PWD, although usually in lower numbers (Kenworthy and Crabb, 1963; Svendsen et al., 1974, 1978). These observations have led to a search for other factors influencing the occurrence of PWD, generally with the presumption that "predisposing factors" allow ETEC to establish themselves in susceptible portions of the intestinal tract in sufficient numbers to initiate diarrhoea. Although many such predisposing factors have been suggested, for example fluctuating environmental temperatures, chilling, crowding etc., the purpose of this paper is only to outline reported influences of diet and closely related factors on susceptibility to PWD, especially in relation to pigs weaned at three to four weeks of age ("early weaning").
Proteolytic enzymes in clinical medicine (1965)

The explosive expansion of our knowledge of the basic properties of the mammalian proteolytic enzymes has stimulated a parallel growth in their applications to clinical medicine. The outcome of this research has been significant advances in the therapy, diagnosis, and pathogenesis of disease processes in almost all fields of medicine. The enzymes discussed in this paper are pepsin, the pancreatic endopeptidases (chymotTypsin, trypsin, kallikrein, and elastase), the blood proteolytic enzymes (thrombin and plasmin), the pancreatic exopeptidases (leucine aminopeptidase and carboxypeptidases), and the tissue proteases (cathepsins). After a brief summary of the physical and biochemical properties of each enzyme, a critical appraisal is made of its potential and limitations as the enzyme is used in clinical medicine. This review concentrates on a delineation of the more important of the recent contributions, a discussion of certain controversial areas, with speCial emphasis on the therapeutic applications and the suggestion of certain fields of investigation which may require more attention in the future.
Actinide Species in Alkaline Media (2000)

Using a variety of different techniques, this program attempts to provide relevant fundamental data that will reduce the number of surprises (related to actinide behavior) that occur during sludge washing and tank waste processing. This work builds upon the results of earlier fundamental studies of actinide chemistry in concentrated alkali (many conducted in Russia), on the analysis of tank wastes, and on earlier sludge-specific leaching experiments to gain an improved understanding of the relationship between sludge characteristics and actinide dissolution during proposed sludge washing procedures. A successful outcome for this research will be the generation of sufficient supporting information (both fundamental and empirical) that will enable more accurate prediction of the probability of actinide dissolution during the scrubbing of sludges from PUREX, REDOX, and BiPO4 processes. We are studying the mobilization of tri-, tetra-, penta-, and hexavalent actinide cations from sludge simulants; trying to identify mineral phases that might control actinide solubility in sludge simulants; learning about associations between actinide cations and non-radioactive sludge components; investigating the solution-phase chemistry of actinide cations in concentrated alkali; and evaluating the contribution of colloid transport to actinide mobility in sludge washes.
Solid-Phase Synthesis of 1,2,3-Trisubstituted Indoles and 2,3-Disubstituted Benzofurans via Iodocyclization (2005)

ADVERTISEMENT RETURN TO ISSUEPREVReportNEXTSolid-Phase Synthesis of 1,2,3-Trisubstituted Indoles and 2,3-Disubstituted Benzofurans via IodocyclizationTuanli Yao, Dawei Yue, and Richard C. LarockView Author Information Department of Chemistry, Iowa State University, Ames, Iowa 50011, and KU Chemical Methodologies and Library Development Center of Excellence, University of Kansas, 1501 Wakarusa Drive, Lawrence, Kansas 66047 Cite this: J. Comb. Chem. 2005, 7, 6, 809–812Publication Date (Web):September 9, 2005Publication History Received9 May 2005Published online9 September 2005Published inissue 1 November 2005https://pubs.acs.org/doi/10.1021/cc050062rhttps://doi.org/10.1021/cc050062rbrief-reportACS PublicationsCopyright © 2005 American Chemical SocietyRequest reuse permissionsArticle Views971Altmetric-Citations47LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Cyclization,Indoles,Organic polymers,Solid phase synthesis,Substituents Get e-Alerts
STORAGE STABILITY OF HIGH TEMPERATURE FUELS. PART I. MICRO FUEL COKER TESTS OF FUEL-RADIOTRACER BLENDS BEFORE STORAGE. (1968)

Abstract : The report describes investigation of the influence of fuel constituents during storage on thermal stability of high-temperature hydrocarbon jet fuels. The study was initiated utilizing a micro fuel coker test apparatus to measure the thermal stability of fuels and the contribution of selected fuel components, labeled with radiotracers, to deposit-forming reactions. Forty-one blends using five fuels and nine radioactive fuel components were prepared, given initial tests and stored at 130F. Aliquots of the five test fuels were depolarized by silica gel percolation and test blends were prepared from these treated fuels for comparison with untreated blends. In a third series of tests, contaminants in the form of red iron oxide and water were added to fuel-radiotracer blends. These 68 blends and approximately 44 additional samples yet to be prepared will be retested after 52 weeks of storage to determine the effect of storage upon deposit-forming tendencies of the labeled fuel compounds. (Author)
Accumulation and Transfer of 32P by Male Southern House Mosquitoes12 (1968)

After 3rd- and 4th-instar larvae of Culex pipiens quinquefasciatus Say were exposed to 32P in water for 48 hours, about 0.86% of the total radioactivity of the male was found in the reproductive organs. Of this amount, about 31% was in the accessory glands and the remainder in the testes, vas deferens, and seminal vesicles. Since the accessory glands make up ⅓ of the volume of the reproductive organs, there was apparently no selectivity on the distribution of 32P in these organs. Approximately 0.09% of the radioactivity found in the male was transferred to the female after mating. Males that averaged 27,000 counts per minute (0.012 μCi) were able to compete favorably with untreated males in inseminating virgin females in the laboratory.
Particle Size and Density Measurement (2020)

This project has developed a miniature gravity settling classifier (flow cell) and flow system that separates slurry particles by settling rate into six different Cuts. The settling rates for particles in each Cut were determined from COMSOL computational fluid dynamics (CFD) modeling of the flow in the flow cell together with particle tracking for particles of differing settling rates. For particles collected in each Cut, the particle concentration and size distribution were measured. The effective particle densities from each Cut are calculated from their respective CFD modeled settling rates using a relationship between the unhindered settling rate, particle size distribution (PSD), and effective density. The particle size and density distribution (PSDD) of the slurry sample is then calculated by combining the size and density distributions determined for the particles collected in each of the settling rate Cuts.
Growth of Single Wall Carbon Nanotubes from Iron Oxide Catalyst (2005)

As one of the most common liver disorders worldwide, nonalcoholic fatty liver disease (NAFLD) begins with the abnormal accumulation of triglyceride (TG) in the liver and can lead to inflammation and fibrosis. Long noncoding RNA (lncRNA) NEAT1 was reported to promote NAFLD progress. However, its molecular mechanism in NAFLD was not fully clear. In vitro cellular model of NAFLD was established with BRL3A cell treated by free fatty acid (FFA). Cell Counting Kit-8 (CCK-8) assay was carried out to assess cell proliferation. The expression of mRNA and protein of inflammation and fibrosis in BRL3A cell was detected by qRT-PCR and Western blot. Bioinformatics and dual-luciferase reporter assays were used to predict and validate the interaction between NEAT1 and miR-506 as well as GLI3 and miR-506. NEAT1 was upregulated while miR-506 was downregulated in the progression of NAFLD. Meanwhile, NEAT1 and miR-506 were proved to regulate fibrosis, inflammatory response, and lipid metabolism. Knockdown of NEAT1 inhibited GLI3 expression and promoted miR-506 expression, Overexpression of miR-506 inhibited NEAT1 and GLI3 expression. Moreover, dual-luciferase reporter assays proved that miR-506 could bind to NEAT1 and GLI3, whereas NEAT1 could sponge miR-506 to regulate GLI3 expression. lncRNA NEAT1 could regulate fibrosis, inflammatory response, and lipid metabolism via the miR-506/GLI3 axis as a ceRNA, which is a novel mechanistic role in the regulation of NAFLD. These results provide a new potential treatment target for NAFLD.
Effects of the Insulation Quality on the Frequency Response of Power Transformers (2006)

This paper presents results of frequency domain spectroscopy (FDS) measurements on oil-impregnated pressboard insulation, their analyses and use of the data for modeling high frequency response (FRA) of transformers. The dielectric responses were measured in a broad frequency range, i.e. from 0.1 mHz to 1 MHz, on model samples containing different amount of moisture. The responses were parameterized with terms representing dc conductivity, low frequency dispersion and Cole-Cole polarization mechanisms and they were thereafter used to model the FRA response of a three-phase transformer.
Electron beam welding of refractory materials for ion propulsion systems (1963)

Electron beam welding was used to solve the problem of joining a porous W emitter to a nonporous W support. A special holding device and jig were designed to hold the parts in position. Three-in. and ten-in. annular ionizers were fabricated successfully in this way. A method was developed for sealing the surface areas around and between the contoured sections in a multistrip porous W emitter, and the emitter was then welded into a manifold. Electronbeam welding was also used successfully to join W, Re, Mo, and Ta to Mo. (D.L.C.)
Anisotropy of the Electrical Conductivity in Bismuth Telluride (1961)

The anisotropy of the electrical conductivity of Bi2Te3 at room temperature has been determined by a four-probe method. The anisotropy ratio for undoped p-type material is close to 3.0; for iodine-doped n-type material it is appreciably higher and increases with the impurity concentration. Measurements of the galvanomagnetic effects in highly doped n-type Bi2Te3 have shown that the observed variation of the anisotropy ratio can be interpreted in terms of a change of shape of the equi-energy surfaces in momentum space with increasing density of charge carriers.
Technik und Morbidität der radikalen inguinal / iliakalen Lymphknotendissektion – eine prospektive Untersuchung an 67 Patienten mit lymphogen metastasiertem malignen Melanom (2009)

Even with a proper perioperative management and a precise wound care management, one-third of the patients undergoing radical inguinal / iliacal lymphadenectomy suffer from a complication requiring medical or interventional treatment. Our data demonstrate that most of these complications can be treated sufficiently by conservative treatment. A fitted surgical support hose could prevent long-term complications.
Basicity and Porosity of a Calcined Hydrotalcite‐Type Material from Nitrogen Porosimetry and Adsorption Microcalorimetry Methods. (2004)

Abstract For Abstract see ChemInform Abstract in Full Text.
Theory of sixth-order wave aberrations: errata (2010)

Get PDF Email Share Share with Facebook Tweet This Post on reddit Share with LinkedIn Add to CiteULike Add to Mendeley Add to BibSonomy Get Citation Copy Citation Text José Sasián, "Theory of sixth-order wave aberrations: errata," Appl. Opt. 49, 6502-6503 (2010) Export Citation BibTex Endnote (RIS) HTML Plain Text Citation alert Save article
Formation evaluation technology for production enhancement. Annual technical report, October 1987-October 1988 (1988)

To determine the reservoir characteristics controlling gas production in the Devonian shale, a systematic means of classifying the various rocks encountered must be made. Eight rock categories based on bulk volume gas, porosity and kerogen content were defined. These were further classified as to being either fractured or non-fractured. A methodology was previously developed to evaluate the reservoir characteristics of the Devonian shales. The methodology involves the computation of the volumetric concentrations of the various constituents and using this information to correct the response of the porosity tools. Once porosity has been determined, the deep resistivity is used to determine the portion of the porosity containing water. This methodology was used in the computation of the wells in the comprehensive study well (CSW) program. Mechanical properties were computed on three shale wells to aid in the design and evaluation of stimulation treatments. Measurements of shear and compressional velocities recorded with an acoustic log were used in the computations along with formation density.