gebhart/patrepeval huggingface dataset:
The weak interdomain coupling observed in the 70 kDa subunit of human replication protein A is unaffected by ssDNA binding (2001)

Replication protein A (RPA) is a heterotrimeric, multi-functional protein that binds single-stranded DNA (ssDNA) and is essential for eukaryotic DNA metabolism. Using heteronuclear NMR methods we have investigated the domain interactions and ssDNA binding of a fragment from the 70 kDa subunit of human RPA (hRPA70). This fragment contains an N-terminal domain (NTD), which is important for hRPA70-protein interactions, connected to a ssDNA-binding domain (SSB1) by a flexible linker (hRPA70(1-326)). Correlation analysis of the amide (1)H and (15)N chemical shifts was used to compare the structure of the NTD and SSB1 in hRPA70(1-326) with two smaller fragments that corresponded to the individual domains. High correlation coefficients verified that the NTD and SSB1 maintained their structures in hRPA70(1-326), indicating weak interdomain coupling. Weak interdomain coupling was also suggested by a comparison of the transverse relaxation rates for hRPA70(1-326) and one of the smaller hRPA70 fragments containing the NTD and the flexible linker (hRPA70(1-168)). We also examined the structure of hRPA70(1-326) after addition of three different ssDNA substrates. Each of these substrates induced specific amide (1)H and/or (15)N chemical shift changes in both the NTD and SSB1. The NTD and SSB1 have similar topologies, leading to the possibility that ssDNA binding induced the chemical shift changes observed for the NTD. To test this hypothesis we monitored the amide (1)H and (15)N chemical shift changes of hRPA70(1-168) after addition of ssDNA. The same amide (1)H and (15)N chemical shift changes were observed for the NTD in hRPA70(1-168) and hRPA70(1-326). The NTD residues with the largest amide (1)H and/or (15)N chemical shift changes were localized to a basic cleft that is important for hRPA70-protein interactions. Based on this relationship, and other available data, we propose a model where binding between the NTD and ssDNA interferes with hRPA70-protein interactions.
Fate of Lymphocytes after Withdrawal of Tofacitinib Treatment (2014)

Tofacitinib (Tofa) is an inhibitor of Janus Kinase 3, developed for the treatment of autoimmune diseases and for the prevention of transplant rejection. Due to its selective action on proliferating cells, Tofa can offer a way to block T cell activation, without toxic effects on resting cells. However, few studies have investigated the effects of Tofa on lymphocyte activation in vitro. Our aim was to study the action of Tofa on different lymphocyte subsets after in vitro stimulation and to track the behaviour of treated cells after interruption of the treatment. Peripheral blood lymphocytes were stimulated in vitro with mitogen and treated with two concentrations of Tofa. After a first period in culture, cells were washed and further incubated for an additional time. Lymphocyte subsets, activation phenotype and proliferation were assessed at the different time frames. As expected, Tofa was able to reduce the activation and proliferation of lymphocytes in the first four days of treatment. In addition the drug led to a relative decrease of Natural Killer, B cells and CD8 T cells compared to CD4 T cells. However, treated cells were still viable after the first period in culture and begun to proliferate, strikingly, in a dose dependent manner when the drug was removed from the environment by replacing the culture medium. This novel data does not necessarily predict a similar behaviour in vivo, but can warn about the clinical use of this drug when a discontinuation of treatment with Tofa is considered for any reason.
Glucose-Responsive Insulin and Delivery Systems: Innovation and Translation (2020)

Type 1 and advanced type 2 diabetes treatment involves daily injections or continuous infusion of exogenous insulin aimed at regulating blood glucose levels in the normoglycemic range. However, current options for insulin therapy are limited by the risk of hypoglycemia and are associated with suboptimal glycemic control outcomes. Therefore, a range of glucose-responsive components that can undergo changes in conformation or show alterations in intermolecular binding capability in response to glucose stimulation has been studied for ultimate integration into closed-loop insulin delivery or “smart insulin” systems. Here, an overview of the evolution and recent progress in the development of molecular approaches for glucose-responsive insulin delivery systems, a rapidly growing subfield of precision medicine, is presented. Three central glucose-responsive moieties, including glucose oxidase, phenylboronic acid, and glucose-binding molecules are examined in detail. Future opportunities and challenges regarding translation are also discussed.
Antigenic Variation in<i>Plasmodium falciparum</i> (2008)

The persistence of the human malaria parasite Plasmodium falciparum during blood stage proliferation in its host depends on the successive expression of variant molecules at the surface of infected erythrocytes. This variation is mediated by the differential control of a family of surface molecules termed PfEMP1 encoded by approximately 60 var genes. Each individual parasite expresses a single var gene at a time, maintaining all other members of the family in a transcriptionally silent state. PfEMP1/var enables parasitized erythrocytes to adhere within the microvasculature, resulting in severe disease. This review highlights key regulatory mechanisms thought to be critical for monoallelic expression of var genes. Antigenic variation is orchestrated by epigenetic factors including monoallelic var transcription at separate spatial domains at the nuclear periphery, differential histone marks on otherwise identical var genes, and var silencing mediated by telomeric heterochromatin. In addition, controversies surrounding var genetic elements in antigenic variation are discussed.
Sodium iodide symporter: its role in nuclear medicine. (2002)

Thyroid iodide uptake is basic to the clinical applications of radioiodine. Iodide uptake occurs across the membrane of the thyroid follicular cells through an active transporter process mediated by the sodium iodide symporter (NIS). The recent cloning of the NIS gene enabled the better characterization of the molecular mechanisms underlying iodide transport, thus opening the way to the clarification and expansion of its role in nuclear medicine. In papillary and follicular carcinoma, NIS immunostaining was positive in only a few tumor cells, and no NIS protein expression was detected in anaplastic carcinomas. Decreased NIS expression levels account for the reduced iodide uptake in thyroid carcinomas. Thus, by targeting NIS expression in cancer cells, we could enable these cells to concentrate iodide from plasma and in so doing offer the possibility of radioiodine therapy. Several investigators have shown that gene transfer of NIS into a variety of cell types confers increased radioiodine uptake by up to several hundredfold that of controls in nonthyroid cancers as well as in thyroid cancer. In addition, my group proposes that NIS may serve as an alternative imaging reporter gene in addition to the HSVtk and dopaminergic receptor genes. The NIS has the potential to expand the role of nuclear medicine in the future, just as it has served as the base for the development of nuclear medicine in the past.
INTERMEDIATE PHASES, REVERSIBILITY WINDOWS, STRESS-FREE AND NON-AGING NETWORKS, AND STRONG LIQUIDS (2008)

Network glasses usually display glass transitions that are hysteretic, i.e., a second scan across a glass transition endotherm usually does not replicate the first one. But in recent years, examination of glass transition endotherms of several inorganic systems [1-4] reveal a different pattern: one observes compositional windows across which glass transitions become almost completely non-hysteretic. Specifically, the non-reversing enthalpy ( Hnr) associated with the glass transition (Tg) accessed from modulated Differential Scanning Calorimetry is found to nearly vanish [1-4]. These compositional windows, also called reversibility windows, usually occur [5] in the 2.29 &lt; r &lt; 2.52 range, where r represents the mean coordination number, a measure of network connectivity. Glasses in reversibility windows form ideal stress-free networks. The ideality derives from the optimization of glass forming tendency. The stress-free character of binary GexSe1-x glasses in the reversibility window, 0.20 &lt; x &lt; 0.25, was recently demonstrated [5] in Raman pressure measurements. In these experiments one found that select vibrational modes blue-shift as a function of external pressure (P), but only once P exceeds a threshold value (Pc). The threshold pressures, Pc, serve as a measure of network internal stress. Chemical trends in Pc(x) are found [5] to closely track
A recess intervention to promote moderate‐to‐vigorous physical activity (2012)

There was no significant difference in BMI and cardiovascular risk factors. A structured recess is feasible to implement and can significantly increase moderate-to-vigorous physical activity.
Antinutrients and natural toxicants in foods (1981)

Proceedings of a 1979 symposium, presented for food scientists and nutritionists, cover 19 research themes in the area of food antinutrients and toxicants. Antinutrients include various materials present in plants that can bind essential minerals, allergens, flatus oligosaccharides, or vitamin antagonists. Toxicants include naturally-occurring toxins, and those that may occur from microbial activity during storage, processing, or cooking. Research themes include: sweet potato mold; the controversy over nitrites and nitrosamines; tremorgenic mycotoxins; cottonseed protein antinutrients; cereal grain hemagglutinins; potato and legume proteinase inhibitors; fluoride intake and retention; dietary fiber effects on zinc bioavailability; and vitamin B6 antagonists in natural products. (wz)
An Abnormal Reaction in the Sommelet Aldehyde Synthesis (1941)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAn Abnormal Reaction in the Sommelet Aldehyde SynthesisReynold C. Fuson and J. J. DentonCite this: J. Am. Chem. Soc. 1941, 63, 3, 654–656Publication Date (Print):March 1, 1941Publication History Published online1 May 2002Published inissue 1 March 1941https://pubs.acs.org/doi/10.1021/ja01848a005https://doi.org/10.1021/ja01848a005research-articleACS PublicationsRequest reuse permissionsArticle Views232Altmetric-Citations8LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Development of an Adaptive Mobile Robot for In-Pipe Inspection Task (2007)

For the purpose of performing internal inspection task of pipe, a robot based on adaptive mobile mechanism is developed. The robot equipped with one actuator has two working modes, a normal working mode and an assistant working mode. Robot under normal working mode performs monitoring tasks and travels in the pipe, while under assistant enhanced mode robot will surmount obstacle without any other driving actuator during executing tasks. The special feature is achieved by applying the adaptive mobile mechanism we proposed in this paper. A prototype has been set up and experiments have been conducted to testify the adaptability and efficiency of the robot. The results show that the robot can successfully climb up a step that is formed by concentric junction with a height of 5 mm.
Definition and Treatment of Systematic Uncertainties in High Energy Physics and Astrophysics (2025)

Systematic uncertainties in high energy physics and astrophysics are often significant contributions to the overall uncertainty in a measurement, in many cases being comparable to the statistical uncertainties. However, consistent definition and practice is elusive, as there are few formal definitions and there exists significant ambiguity in what is defined as a systematic and statistical uncertainty in a given analysis. I will describe current practice, and recommend a definition and classification of systematic uncertainties that allows one to treat these sources of uncertainty in a consistent and robust fashion. Classical and Bayesian approaches will be contrasted.
FIFTY YEARS OF RACE RELATIONS IN EAST ST. LOUIS: THE BREAKING DOWN OF WHITE SUPREMACY (1965)

In 1917 at East St. Louis, Illinois, a community then popularly known as the offshoot of St. Louis, 1T the bloodiest race riot in twentieth century America took place. Nine whites and at least thirty-nine Negroes were killed. The whites were determined to maintain supremacy in a community where Negroes were a subordinate and segregated group. For more than a generation after 1917, the race riot was used as a cautionary tale to keep Negroes from altering the status quo. Not until after the Second World War did Negroes feel free to consider challenging white dominance. In violation of state laws, in 1917 East St. Louis Negroes were excluded from restaurants, theaters and hotels, and were educated in separate schools. Both the AFL and the large industrial corporations excluded Negroes from skilled jobs. In the big factories unskilled colored employees used separate washrooms and dressing rooms, usually worked in segregated labor gangs and ate in the colored section of the lunchrooms . Most Negroes resigned themselves to accepting the status quo, and the few who agitated for racial equality were condemned by local whites who charged them with preaching discontent to the rest of the colored residents. One militant Negro, who was labeled an aggressive political agitator, fl was falsely convicted of starting the 1917 race riot and sentenced to life imprisonment. 1 The background of the East St. Louis racial conflagration was laid in the migration of several thousand Negroes who arrived from the South between 1915 and 1917 to work in the factories. At the time of America's
Selectivity of radical formation in the reaction of carbonyl compounds with manganese(III) acetate (1987)

The selectivity of radical generation in the oxidation of carbonyl compounds by manganese(III) has been studied in two ways. Competitive reaction of acetic and propionic acids under conditions in which the resultant radicals are trapped efficiently by an alkene suggests that propionic acid reacts some 3.8 times more readily than does acetic acid. Oxidation of butanone under similar conditions gives products in the formation of which reaction has occurred 2.9 times more readily at C-3 of butanone than at C-1. However, this ratio reflects a combination of chain propagation and of initiation by manganese(III) oxidation. When propagation is effectively eliminated, the C-1 position is the more reactive. The significance of these observations on the mechanism of oxidation of carbonyl compounds by manganese(III) is discussed. It is suggested that enol radical-cations can be formed in the reaction but that these do not give carbonyl-stabilised radicals by deprotonation.
Accessing Targeted Nanoparticles to the Brain: The Vascular Route (2014)

The blood-brain barrier (BBB), formed by brain capillary endothelial cells, prevents the entry of several drug molecules to the brain, especially molecules hydrophilic in nature. Advanced drug carriers like nanoparticles share the potential to allow entry of therapeutic proteins and genetic molecules into the central nervous system (CNS). Taking a targeting approach by conjugating molecules acting as ligands or monoclonal antibodies with affinity for proteins expressed on the luminal side of brain capillary endothelial cells, the nanoparticles can be designed to enable transport into the brain endothelium, or perhaps even through the endothelium leading to blood to brain transport. Currently, the iron-binding protein transferrin or antibodies raised against the transferrin receptor denote the most feasible molecule for targeting purposes at the BBB. This manuscript reviews the targetability of nanoparticles to the brain capillary endothelial cells, how nanocarriers may enter and transfer through the brain endothelium, and how likely restraints denoted by the threedimensional mesh of the extracellular proteins forming the brain capillary basement membrane challenge the possibilities for enabling transport of large molecules through the BBB encapsulated in nanoparticles.
FILMS (1983)

Most trainers will be acquainted with some of Video Arts' training films. Having won major awards, and using the best actors, script writers and techniques, they have achieved much of note in training in recent years. Video Arts are not ten years old (surely, you say, they must have been going much longer!) and we thought it would be helpful to trainers to bring together short descriptions of some of their major films.
Personal Protective Equipment Decontamination for Hazardous Waste Operations and Emergency Response (1992)

Decontamination of personal protective equipment requires knowledge of the contaminant, methods of detection for contamination, and use of effective materials and procedures for decontamination. Contamination can be of a surface, matrix, or pore type. Each must be handled differently to achieve effective decontamination. Tests for contamination range from simple visual methods to destructive, complex, and very expensive analytical protocols. Methods of decontamination can be either physical or chemical. In general, physical methods can be effective for surface contaminants that can be mechanically dislodged or dissolved. Heating and hot air washing can be effective for volatiles as surface and matrix contaminants. Chemical methods are usually effective for a limited number of surface contaminants. Pore contaminants are extremely difficult to decontaminate by any nondestructive method. The best approach to decontamination is to minimize the need to perform it. This is accomplished by minimizing workers and equipment in hazardous areas and avoiding the use of porous equipment and materials.
Computer control of a robotic satellite servicer (1980)

The advantages that will accrue from the in-orbit servicing of satellites are listed. It is noted that in a concept in satellite servicing which holds promise as a compromise between the high flexibility and adaptability of manned vehicles and the lower cost of an unmanned vehicle involves an unmanned servicer carrying a remotely supervised robotic manipulator arm. Because of deficiencies in sensor technology, robot servicing would require that satellites be designed according to a modular concept. A description is given of the servicer simulation hardware, the computer and interface hardware, and the software. It is noted that several areas require further development; these include automated docking, modularization of satellite design, reliable connector and latching mechanisms, development of manipulators for space environments, and development of automated diagnostic techniques.
Lacunary polyoxometalate anions are .pi.-acceptor ligands. Characterization of some tungstoruthenate(II,III,IV,V) heteropolyanions and their atom-transfer reactivity (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTLacunary polyoxometalate anions are .pi.-acceptor ligands. Characterization of some tungstoruthenate(II,III,IV,V) heteropolyanions and their atom-transfer reactivityChaoying Rong and Michael T. PopeCite this: J. Am. Chem. Soc. 1992, 114, 8, 2932–2938Publication Date (Print):April 1, 1992Publication History Published online1 May 2002Published inissue 1 April 1992https://pubs.acs.org/doi/10.1021/ja00034a027https://doi.org/10.1021/ja00034a027research-articleACS PublicationsRequest reuse permissionsArticle Views1838Altmetric-Citations215LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
6.8 A 100Gb/s NRZ Transmitter with 8-Tap FFE Using a 7b DAC in 40nm CMOS (2020)

Recently, PAM-4 transmitters have been realized at 112Gb/s or even faster to satisfy the continuously growing demands for wireline communications [1]-[5]. Although PAM-4 signaling performs two-fold bandwidth efficiency compared with the NRZ counterpart, the NRZ signal still has the advantage in low-loss scenarios like ultra-short-reach (USR) interconnection for optical engines due to its high output swing and better SNR. This paper presents a 100Gb/s NRZ transmitter fabricated in 40nm CMOS by incorporating a 7b DAC with an 8-tap FFE implemented in DSP. The DAC-based architecture demonstrates high resolution for FFE coefficients, achieving an output eye diagram with 73mV eye height and 760fs <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">rms</sub> jitter under 7.1dB loss at 50GHz while consuming 619mW of power from 1.1V/1.2V/1.5V supplies.
Hereditary congenital heart defects in dogs (1989)

ABSTRACT Congenital heart defects is probably the most common class of malformations found in dogs, occurring with a frequency approaching 1 per cent in animals presented to veterinary clinics. The frequency is significantly higher among purebred dogs than in dogs of mixed breeding and specific anatomic malformations occur with highest frequency in certain breeds. Genetic studies of patent ductus arteriosus, pulmonic stenosis, subaortic stenosis, ventricular septal defect, tetralogy of Fallot and persistent aortic arch have confirmed that these are specific heritable defects, the genes for which are concentrated in a number of different breeds. Each of these defects is inherited in a complex manner consistent with a polygenic basis. This paper will describe evidence supporting the view that the common forms of congenital heart disease in the dog are polygenic threshold traits. The general criteria for recognition of polygenic traits and methods for their control will be discussed.
Soccer injuries to the brain (1991)

Thirty-seven former soccer players of the National Football Team of Norway were individually examined with an extensive battery of psychologic tests. The neuropsychologic examination demonstrated mild to severe deficits regarding attention, concentration, memory, and judgment in 81% of the players. This may indicate some degree of permanent organic brain damage, probably the cumulative result of repeated traumas from heading the ball.