gebhart/patrepeval huggingface dataset:
Cell-sized lipid vesicles for cell-cell synaptic therapies (2017)

Cell-sized lipid vesicles (CLVs) have shown great promise for therapeutic and artificial cell applications, but their fragility and short shelf life has hindered widespread adoption and commercial viability. We present a method to circumvent the storage limitations of CLVs such as giant unilamellar vesicles (GUVs) and single-compartment multisomes (SCMs) by storing them in a double emulsion precursor form. The double emulsions can be stored for at least 8 months and readily converted into either GUVs or SCMs at any time. In this study, we investigate the interfacial parameters responsible for this morphological change, and we also demonstrate the therapeutic potential of CLVs by utilizing them to present a transmembrane protein, neuroligin-2, to pancreatic β-cells, forming cell-cell synapses that stimulate insulin secretion and cellular growth.
An Elementary Introduction to Sato Theory (1988)

An .elementary introduction to Sato theory is given. Starting with an ordinary differential equation, introducing an infinite number of time variables, and imposing a certain time dependence on the solutions, we obtain the Sato equation which governs the time development of the variable coefficients. It is shown that the generalized Lax equation, the Zakharov-Shabat equation and the IST scheme are generated from the Sato equation. It is revealed that the r-function becomes the key function to express the solutions of the Sato equation. By using the results of the representation theory of groups, it is shown that the r-function is governed by the partial differential equations in the bilinear forms which are closely related to the PlUcker relations.
Comparative GC/MS Analysis of Rose Flower and Distilled Oil Volatiles of The Oil Bearing Rose<i>Rosa Damascena</i> (2011)

ABSTRACTThe accelerated and successful oil bearing rose breeding requires routine application of efficient procedure for analysis of flower volatiles, with capacity to extrapolate the obtained flower data to the volatile composition of the distilled rose oil. In the current study a procedure for solvent extraction and GC/MS analysis of rose flower and rose oil volatiles from oil bearing roses including Rosa damascena is presented. The procedure allows reliable identification of 68 volatiles in the rose flowers which are also detected in the distilled rose oil. The described procedure was further applied for comparative analysis of the flower and distilled rose oil volatiles from eight different genotypes of oil bearing roses. A data set consisting of ratios of the relative abundance of given volatile in the flower spectra to the relative abundance of the same volatile in the distilled rose oil spectra was generated. ANOVA test for a data subset of 27 volatiles detected in the flowers and rose oils of all analyzed oil bearing rose genotypes showed no significant influence of the genotype on the ratio of relative abundances of flower to rose oil volatiles. The average and relative standard deviation values of the obtained ratios between relative abundances of flower and rose oil volatiles for the analyzed genotypes were calculated for each identified flower compound. The results demonstrate that the described flower solvent extraction and GC/MS analysis procedure could be reliably applied for prediction of the volatile composition of distilled rose oils from wide range of oil bearing rose genotypes based on the extrapolation of GC/MS analysis data from single or few flowers from each studied plant. The possibilities for incorporation of the described procedure into oil bearing rose breeding and genetic resources characterization are discussed.Keywords: oil bearing roseRosa damascenavolatilesflowersolvent extractionrose oilGC/MS
Contamination of rat urine with gut flora using all-glass metabolism cages for collection of urine and faeces (1977)

In rat urine collected in all-glass metabolism cages, at least four strains of intestinal microflora were found: two types of E. coli, Enterobacter cloaceae and Proteus vulgaris. The number of bacteria of each strain increased with time. 2. The pH of the urine increased from 6-9 after 24 h to 8-95 after 120 h. The pH of the urine of neomycin-treated rats remained nearly constant over a period of two days. 3. Nitroreductase activity was present in the rat urine. Added p-nitrobenzoic acid was reduced within the first 24 h. Nitroreductase activity in the urine of neomycin-treated rats was significantly lower than in the urine of normal rats, during the second 24-h period only. 4. Collection of urine at -10 degrees prevented the consequences of contamination.
The Impact of Nonclinical Factors on Care Use for Patients with Depression: A STAR*D Report (2009)

Data suggest diligent, measurement-based mental health programs may reduce race, gender, and education disparities in the use of needed mental health care.
Pulmonary Blood Flow Redistribution with Low Levels of Positive End-Expiratory Pressure (1999)

KALLAS, HARRY J.; DOMINO, KAREN B.; GLENNY, ROBB W.; ANDERSON, EMILY A.; HLASTALA, MICHAEL P. Author Information
Nuclear Receptor Steroidogenic Factor 1 Directs Embryonic Stem Cells toward the Steroidogenic Lineage (1997)

The orphan nuclear receptor steroidogenic factor 1 (SF-1) is expressed in the adrenal gland and gonads and is an important regulator of the expression of cytochrome P-450 steroidogenic enzymes in cultured cells. Targeted disruption of the SF-1 gene in mice shows that it is a critical participant in the genetic program that promotes the development of urogenital mesoderm into the adrenal gland and gonads. To assess the ability of SF-1 to regulate this differentiation pathway, we ectopically expressed SF-1 in murine embryonic stem (ES) cells. We found that stable expression of SF-1 is sufficient to alter ES cell morphology, permit cyclic AMP (cAMP) and retinoic acid-induced expression of the endogenous side chain cleavage enzyme gene, and consequently, promote steroidogenesis. While steroid production is dependent upon SF-1, cAMP induction of steroidogenesis does not enhance the responsiveness of an SF-1-specific reporter. Furthermore, the activity of a P450SCC promoter/luciferase reporter construct, which is induced by cAMP in steroidogenic cells and ES cells converted by stable expression of SF-1, is not induced by cAMP in wild-type ES cells transiently transfected with SF-1, suggesting that the induction of downstream gene products is required before steroidogenesis can occur. We demonstrate that mutants which disrupt the DNA binding domain or the AF2 transcriptional activation domain of SF-1 do not confer the steroidogenic phenotype to ES cells. Interestingly, however, AF2 mutants fused to the VP16 activation domain do confer the steroidogenic phenotype to ES cells, but only in the presence of a portion of the ligand binding domain. These studies extend the role of SF-1 in steroidogenic tissues to that of a dominant regulator of the steroidogenic cell phenotype.
Ionospheric calibration of radar altimeters using GPS tomography (1998)

We compare TEC measurements from the NASA Radar Altimeter and DORIS instrument on board TOPEX/POSEIDON with GPS TEC estimates, and evaluate different GPS data analysis strategies. We verify that global tomographic GPS analysis using a voxel grid is well suited for ionospheric calibration of altimeters. We show that a 1‐day fit of 20‐second‐averaged NRA ionospheric correction data versus GPS tomographic TEC data has a bias of 3.4 TECU and a root mean square deviation of 3.2 TECU. Tomographic inversion using simulated data from the Parametrized Ionospheric Model highlights the strong correlation between GPS bias constants, electronic densities at the highest layer, and unmodeled protonospheric TEC. This suggests that GPS TEC estimates at the TOPEX/POSEIDON altitude are more accurate if the bias constants are estimated and if a layer above TOPEX/POSEIDON is added to the grid.
<i>FIDDLEHEAD</i> , a gene required to suppress epidermal cell interactions in <i>Arabidopsis</i> , encodes a putative lipid biosynthetic enzyme (2000)

In plants, the outer epidermal cell wall and cuticle presents a semipermeable barrier that maintains the external integrity of the plant and regulates the passage of various classes of molecules into and out of the organism. During vegetative development, the epidermal cells remain relatively inert, failing to respond to wounding or grafting. During reproductive development and fertilization, however, the epidermis is developmentally more labile and participates in two types of contact-mediated cell interactions: organ fusion and pollen hydration. Here we describe the isolation and characterization of one gene whose product normally functions in blocking both types of epidermal cell interactions during vegetative development: the FIDDLEHEAD gene. As suggested by previous biochemical analyses, the gene encodes a protein that is probably involved in the synthesis of long-chain lipids found in the cuticle and shows similarity to a large class of genes encoding proteins related to beta-ketoacyl-CoA synthases and chalcone synthases. In situ hybridization reveals an epidermal pattern of expression consistent with a role for this protein in the synthesis of lipid components that are thought to localize extracellularly and probably modify the properties of the cuticle.
Room-temperature reproducible spatial force spectroscopy using atom-tracking technique (2005)

A method for reproducible site-specific force spectroscopic measurements using frequency modulation atomic force microscopy at room temperature is presented. The stability and reproducibility requirements, fulfilled so far only in cryogenic environment, are provided through the compensation of the thermal drift using the atom-tracking technique. The method has been tested performing spectroscopic measurements on atomic positions of the Si(111)-(7×7) surface with Si tips. The room-temperature results presented here compare in quality to previously reported quantitative force spectroscopic data obtained at cryogenic temperatures.
Efficient One‐Pot Formation of 4‐N‐Substituted 2,4‐Dihydro‐3H‐1,2,4‐triazolin‐3‐ones from Primary Amines Using N′‐(Ethoxymethylene)hydrazinecarboxylic Acid Methyl Ester. (2006)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Comparing cortisol, stress, and sensory sensitivity in children with autism (2009)

Previously we reported that children with autism show significant variability in cortisol. The current investigation was designed to extend these findings by exploring plausible relationships between cortisol and psychological measures of stress and sensory functioning. Salivary cortisol values for diurnal rhythms and response to stress in children with and without autism were compared to parent-report measures of child stress, the Stress Survey Schedule (SSS), sensory functioning, Short Sensory Profile (SSP), and Parenting Stress Index. In autism, a negative relationship between morning cortisol and the SSS revealed that higher observed symptoms of stress were related to lower cortisol. Lower cortisol is seen in conditions of chronic stress and in social situations characterized by unstable social relationships. Sensory sensitivity painted a more complicated picture, in that some aspects of SSP were associated with higher while others were associated with lower cortisol. We propose that increased sensory sensitivity may enhance the autistic child's susceptibility to the influence of zeitgeibers reflected in variable cortisol secretion. Evening cortisol was positively associated with SSS such that the higher the level of evening cortisol, the higher the child's parent-reported daily stress, especially to changes, such as in daily routine. Regarding the response to stress, the psychological and parent variables did not differentiate the groups; rather, discrete subgroups of cortisol responders and nonresponders were revealed in both the autism and neurotypical children. The results support a complex interplay between physiological and behavioral stress and sensory sensitivity in autism and plausible developmental factors influencing stress reactivity across the groups.
Geodesic sphere phased arrays for LEO satellite communications (2002)

Cellular scanning by geodesic sphere phased arrays-a new electronic scanning concept with a new phased array architecture is introduced. The new phased array consists of N number of substantially equilateral triangular shaped planar subarrays arranged in the geodesic sphere configuration that will provide coverage of the entire sky for multi-satellite communication. Adjacent vertices of the geodesic sphere define the cellular communication space. Preliminary design calculations are presented to estimate the construction parameters for an application.
Relative concentration of 13,14-dihydro-15 keto-prostaglandin F-2α in blood and milk of buffaloes during the oestrous cycle and early pregnancy (1983)

Summary. Concentrations of PGFM in blood and milk of 28 Murrah buffaloes increased significantly (P &lt; 0·001) in non-pregnant animals before oestrus but not in pregnant animals. The PGFM level in milk was similar to that in blood plasma and was positively correlated in non-pregnant (r = 0·981) and pregnant (r = 0·521) animals. Mean blood plasma PGFM concentration was not significantly (P &lt; 0·05) different from that of milk.
653: Targeted Screening in High Risk Families: High Detection Rate and Positive Predictive Value of Increased PSA (>4NG/ML) in 2+ and Early Onset Prostate Cancer Families (2004)

You have accessJournal of UrologyDiscussed Poster, Sunday, May 9, 2004, 1:00 - 5:00 pm1 Apr 2004653: Targeted Screening in High Risk Families: High Detection Rate and Positive Predictive Value of Increased PSA (>4NG/ML) in 2+ and Early Onset Prostate Cancer Families Antoine Valeri, Marie Pierre Moineau, Vincent Joulin, Luc Cormier, Rahmene Azzouzi, Laurent Doucet, Philippe Mangin, Olivier Cussenot, Jean Francois Morin, and Georges Fournier Antoine ValeriAntoine Valeri More articles by this author , Marie Pierre MoineauMarie Pierre Moineau More articles by this author , Vincent JoulinVincent Joulin More articles by this author , Luc CormierLuc Cormier More articles by this author , Rahmene AzzouziRahmene Azzouzi More articles by this author , Laurent DoucetLaurent Doucet More articles by this author , Philippe ManginPhilippe Mangin More articles by this author , Olivier CussenotOlivier Cussenot More articles by this author , Jean Francois MorinJean Francois Morin More articles by this author , and Georges FournierGeorges Fournier More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(18)37915-1AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail "653: Targeted Screening in High Risk Families: High Detection Rate and Positive Predictive Value of Increased PSA (>4NG/ML) in 2+ and Early Onset Prostate Cancer Families." The Journal of Urology, 171(4S), p. 174 © 2016 by American Urological AssociationFiguresReferencesRelatedDetails Volume 171Issue 4SApril 2004Page: 174 Advertisement Copyright & Permissions© 2016 by American Urological AssociationMetricsAuthor Information Antoine Valeri More articles by this author Marie Pierre Moineau More articles by this author Vincent Joulin More articles by this author Luc Cormier More articles by this author Rahmene Azzouzi More articles by this author Laurent Doucet More articles by this author Philippe Mangin More articles by this author Olivier Cussenot More articles by this author Jean Francois Morin More articles by this author Georges Fournier More articles by this author Expand All Advertisement PDF DownloadLoading ...
A patient with MV2 subtype of sporadic Creutzfeldt-Jakob disease and atypical clinical presentation (2008)

We report the case of a 71-year-old woman with progressive dementia over the course of 4 years, characterized by prominent pyramidal signs and by the lack of ataxia and other cerebellar signs. Creutzfeldt-Jakob disease (CJD) was not suspected during the patient's life. Autopsy brain tissue showed severe spongiform encephalopathy with kuru-like, but not florid, plaques in neocortex and cerebellum. Massive synaptic diffuse and plaque-like PrP(Sc) deposition was found in the cerebral cortex, striatum, cerebellum and brainstem. Genetic analysis revealed no PRNP gene mutations and methionine/valine heterozygosity (MV) at codon 129. The pathogenic scrapie prion protein (PrP(Sc)) pattern detected by Western blot was Type 2. However, this pattern showed a single unglycosylated band in contrast to the doublet described for MV2 subtype of sCJD with kuru plaques. In summary, this is an autopsy case report of a particular presentation of MV2 subtype of sCJD.
ChemInform Abstract: SYNTHESIS AND ENZYMIC ACTIVITY OF VARIOUS SUBSTITUTED PYRAZOLO(1,5‐A)‐1,3,5‐TRIAZINES AS ADENOSINE CYCLIC 3′,5′‐PHOSPHATE PHOSPHODIESTERASE INHIBITORS (1982)

Abstract Auf den im Formelschema beschriebenen Wegen werden Pyrazolo‐1,3,5‐triazine hergestellt.
Study on an inter-organization activity based on the Internet traffic (2003)

A traffic analysis on the Internet has an advantage for obtaining the characteristics of transferred packets. There were many studies to understand the characteristics of the Internet traffic with mathematical statistical approach. The approach of this study is different front previous studies. It focuses on studying the traffic analysis with the framework of knowledge extraction. The measurement results of traffic capturing on the backbone network is applied to the inter-organization activity on the Internet. It shows that the proposed framework can be useful for the experiment results.
Adaptive Antenna Array Interference Mitigation Diversity for Decentralized Dynamic Spectrum Allocation in License-Exempt Spectrum (2009)

Decentralized dynamic spectrum allocation (DSA) that exploit adaptive antenna array interference mitigation (IM) diversity at the receiver, is proposed for interference-limited environments with high level of frequency reuse. The system consists of base stations (BSs) that may belong to different providers in license-exempt spectrum, who can optimize uplink frequency allocation to their subscriber stations (SSs) to achieve the least impact of IM on the useful signal, assuming no control over band allocation of other BSs sharing the same bands. The IM-based DSA algorithms based on a "good neighbor" decentralized DSA strategies are proposed. The new strategy introduces controllable behavior of decentralized networks, which promises a practical perspective for IM-based DSA algorithms even without guarantee of the network convergence to stationary points.
Intra–operative Sensemaking by Surgeons: Analysis of Operative Reports for Uncertainty and Irregular Cues (2009)

Motivation – Understanding surgeons’ intraoperative sensemaking and whether linguistic and descriptive choices reflect unconscious thought. Research approach – Analysis of surgeons’ sensemaking, using laparoscopic cholecystectomy operative reports of cases with and without bile duct injuries, examining for linguistic modifiers of uncertainty and description of irregular cues. Findings/Design – qualifiers of uncertainty and an increased number of irregular cues were more common in bile duct injury cases. Research Implications – These data suggest the unconscious mind detects aberrations whose significance cannot break through to conscious recognition. Originality/Value – Both complicated and uncomplicated cases were studied. The importance of linguistic qualifiers has not been previously studied in this domain. Take away message – descriptive and linguistic choices reflect unconscious thought.
One Centimeter Spatial Resolution Temperature Measurements from 25 to 850°C Using Rayleigh Scatter in Gold Coated Fiber (2007)

We present the use of swept wavelength interferometry for distributed fiber-optic, temperature measurements of up to 850degC over a 1m fiber segment in commercially available, single mode, gold coated fiber. The interrogation technique is based on measuring the spectral shift of the intrinsic Rayleigh backscatter signal along the optical fiber and converting the spectral shift to temperature.
Cleavage of single stranded oligonucleotides by<i>Eco</i>RI restriction endonuclease (1987)

The 31mer 5'-TCA ACG CTA GAA TTC GGA TCC ATC GCT TGG T, the complementary 33mer 5'-CCA AGC GAT GGA TCC GAA TTC TAG CGT TGA GAT, the 40mer 5'-GGC CAG GAT GGT GAA GAA TTC GAT CCG GTA CGT AGC TAA G, and the complementary 42mer 5'-TAC TTA GCT ACG TAC CGG ATC GAA TTC TTC ACC ATC CTG GCC were synthesized and their reactivity towards EcoRI was studied. It was found that the 31mer and the 40mer were cleaved at a comparable rate to the 31mer-33mer hybrid and the 40mer-42mer hybrid, respectively. The rate of cleavage of the 33mer and the 42mer was an order of magnitude lower. To rule out possible intermolecular duplex formation, the 33mer was immobilized on cellulose by ligation and labeled with alpha 32P-dCTP using Klenow fragment of E. coli DNA polymerase. EcoRI cleaved this immobilized oligomer into specific fragments.
Prophylactic immunity in the mealworm beetle Tenebrio molitor. (2001)

Immune systems are thought to be costly to maintain and express in a variety of taxa.\nEvidence for this comes from observations that mechanisms which deal with pathogenic\nchallenge are often extremely variable and are induced in the presence of an immune\nchallenge, rather than being constitutively active. This thesis presents work aimed at\ntesting predictions arising from these ideas, using the mealworm beetle T. molilor\n(Coleoptera: Tenebrionidae) as a model system. Assays were developed which reflected\nthree specific aspects of immunity (haemocyte count, phenoloxidase levels and resistance\nto a generalist fungal pathogen), thereby allowing aspects of immune function to be\nquantified. It was shown that the level of fungal resistance differed in beetles reared\ngregariously (higher resistance) and solitarily (lower resistance). Conspecifics are a\nsource of disease (Freeland 1983), so this is an example of immune defences being\ninduced in situations with a higher risk of pathogenesis (density dependent prophylaxis).\nA strong predictor of fungal resistance was the degree of melanisation of the adults'\ncuticles. This trait was shown to be highly heritable (59%), as was the total haemocyte\ncount of an individual: an important aspect of general invertebrate immunity. Selection\nfor cuticular melanisation resulted in a rapid response, confirming the existence of large\namounts of additive genetic variance for this trait. Fungal resistance showed a correlated\n111 \nresponse to selection for cuticular melanisation, indicating that this too has additive\ngenetic variance. Lines selected for darker cuticles showed higher levels of fungal\nresistance than those selected for lighter cuticles. Cuticular melanisation and fungal\nresistance are therefore genetically correlated, and the former can be used as an indicator\nof the latter in T. molitor. No specific costs of cuticular melanisation or fungal resistance\nwere identified. A correlated response to selection for cuticular melanisation on larval\ncompetitive ability was investigated, but no such response was seen. Thus the\nmechanisms maintaining variability and inducibility in cuticular melanisation and fungal\nresistance are unknown. This thesis has therefore identified patterns of immune\nexpression consistent with the hypothesis that immunity has associated costs, although\nthese costs have not been shown. It has also identified a potentially novel role for\ncuticular melanisation, as an indicator of immunity to fungal pathogens.