gebhart/patrepeval huggingface dataset:
Epidermal tight junction: The master skin barrier regulator (2010)

IFSCC Magazine , 12 (2009) (2) 87–91 Tight junctions between adjacent epithelial cells control paracellular permeability of solutes and maintain cell polarity by fencing lipid bilayer components into demarcated apical and basolateral domains. In the present study we investigated the role of epidermal tight junctions in two skin physiological processes, ‘formation of the epidermal calcium ion (Ca 2+ ) gradient’ and ‘polarized lamellar body secretion’. The former is based on the fact that the epidermal Ca2+ gradient is closely related to epidermal differentiation and the latter on the knowledge that polarized lamellar body secretion is essential to supply intercellular lipids to the stratum corneum. We hypothesized that tight junctions might form the Ca 2+ gradient in the epidermis by sealing cell‐cell contact at the stratum granulosum and allow the lamellar bodies to be normally secreted toward the stratum corneum by giving granular cells polarity. In our experiments we discovered that tight junctions control not only both intercellular Ca 2+ permeability and fluorescent ceramide analog (Cer‐FL) secretion in cultured normal human keratinocyte cells but also both intercellular calcium distribution and polarized lamellar body secretion in the skin equivalent. Thus, tight junctions should be responsible for linking a series of processes from epidermal differentiation to stratum corneum barrier formation. Our findings suggest that tight junctions should have a crucial role not only in epidermal barrier function but also in both epidermal differentiation and stratum corneum barrier function. Keywords: Calcium gradient, cell polarity, lamellar body, skin barrier, tight junction IFSCC Basic Research Award winning paper presented at the 25th IFSCC Congress 2008, Barcelona, Spain
Microwave Absorption in the Mixture (Phenyl Isothiocyanate+Diethylamine) (1954)

Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter Facebook Reddit LinkedIn Tools Icon Tools Reprints and Permissions Cite Icon Cite Search Site Citation P. K. Kadaba; Microwave Absorption in the Mixture (Phenyl Isothiocyanate+Diethylamine). J. Chem. Phys. 1 August 1954; 22 (8): 1465–1466. https://doi.org/10.1063/1.1740429 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAIP Publishing PortfolioThe Journal of Chemical Physics Search Advanced Search |Citation Search
Finite Element Models and Properties of a Stiffened Floor-Equipped Composite Cylinder (2010)

Finite element models were developed of a floor-equipped, frame and stringer stiffened composite cylinder including a coarse finite element model of the structural components, a coarse finite element model of the acoustic cavities above and below the beam-supported plywood floor, and two dense models consisting of only the structural components. The report summarizes the geometry, the element properties, the material and mechanical properties, the beam cross-section characteristics, the beam element representations and the boundary conditions of the composite cylinder models. The expressions used to calculate the group speeds for the cylinder components are presented.
Implementation and Dissemination of a Military Trauma System: Utilizing Medical Lessons Learned from the Battlefield (2010)

Abstract : Derived from the necessity to mitigate injury outcomes across the spectrum of battlefield injury, the Joint Theater Trauma System (JTTS) and Joint Theater Trauma Registry (JTTR) were developed utilizing the American College of Surgeons Committee on Trauma Optimal Resources model in accordance with the U.S. civilian trauma system approach. This analysis was developed to highlight successes of the JTTS. The JTTR captured demographic, mechanistic, physiologic, diagnostic, therapeutic, and outcome data on 23,754 casualty injury events from October 2001 through December 2008 for this analysis. In contrast to civilian trauma systems, the majority of battlefield wounds were penetrating mechanism (66.4%). Of patients admitted to deployed military medical treatment facilities, 23.4% had an injury severity score (ISS) /=16, 21+/-4% presented in shock (base deficit 5), 30+/-3 % patients required blood and 6.8+/-2% required massive transfusion. In response to this complex and severely injured population, the JTTS remedied numerous trauma system issues requiring system leadership and advocacy, education, research, and alterations in clinical care, including the elaboration of 31 evidence-based clinical practice guidelines. Several of the guidelines including those for massive transfusion / damage control resuscitation, burn care, hypothermia prevention and management, wound and amputation management have been associated with demonstrable improvements in combat injury outcomes. The JTTS developed a novel performance improvement process across the global continuum of care, a process responsible for improved battlefield injury survival, notably a mortality rate of 4.1% after admission which is comparable to the U.S. civilian standards. In addition, the JTTS fostered the development of military trauma systems and combat trauma registries in several NATO coalition partners including Canada and the United Kingdom.
Direct detection of markers associated with<i>Neisseria gonorrhoeae</i>antimicrobial resistance in New Zealand using residual DNA from the Cobas 4800 CT/NG NAAT assay: Table 1 (2014)

The use of residual clinical DNA samples from the Cobas 4800 CT/NG test proved an efficient and effective method for performing AMR genotyping. These data also show for the first time the presence of gonococci with a mosaic penA sequence in New Zealand. Overall, the results further highlight the potential of molecular methods to aid N. gonorrhoeae AMR surveillance, particularly for regions where gonococcal culture is no longer performed.
A DyI3-catalyzed Mannich-type Reaction of 1-Methylcyclopropanecarboxylate-type Donors for the Stereoselective Synthesis of Pyrrolidines with Quaternary Stereocenters (2008)

Abstract Stereoselective synthesis of functionalized pyrrolidines with all-carbon quaternary stereocenters is described. DyI3 catalyzed the ring opening of cyclopropanecarboxylate equivalents and promoted Mannich-type addition of the resulting α,α-disubstituted enolate intermediates to aryl and isomerizable alkyl imines, giving products in 86–58% yield and &gt;96:4–84:16 diastereoselectivity.
Comments on Article by Tran and Colleagues, "Double-Blind Comparison of Olanzapine Versus Risperidone in Treatment of Schizophrenia and Other Psychotic Disorders" (1998)

Lilly Research Laboratories, Clinical Research Division, Lilly Corporate Center, Indianapolis, Indiana 46285.
Novel narrowband acoustic sensors for sub-GHz wireless measurements (2011)

High-overtone Bulk Acoustic Resonator is an acoustic transducer based on an excitation of a bulk acoustic wave by a thin piezoelectric film bonded to a thick low acoustic loss substrate. This combination of materials aims at providing on the one hand a high frequency transducer as defined by the thickness of the thin piezoelectric layer, and on the other hand the robustness of a thick substrate while keeping the acoustic properties of single crystal piezoelectric materials. More specifically, this architecture provides high quality factors using bulk acoustic wave at frequencies only accessible to surface acoustic wave (SAW) devices with interdigitated transducer generation. The multimode spectrum is well suited for an openloop, wireless interrogation strategy in which the frequency of the incoming electromagnetic wave defines the operating point. We here demonstrate the use of a frequency sweep RADAR-like network analyzer for probing through a wireless link HBARs with different temperature coefficients in order to perform temperature measurements insensitive to other correlated noise sources (capacitive frequency pulling, electrode aging, stress).
Biological Atomic Force Microscopy for Imaging Gold-Labeled Liposomes on Human Coronary Artery Endothelial Cells (2013)

Although atomic force microscopy (AFM) has been used extensively to characterize cell membrane structure and cellular processes such as endocytosis and exocytosis, the corrugated surface of the cell membrane hinders the visualization of extracellular entities, such as liposomes, that may interact with the cell. To overcome this barrier, we used 90 nm nanogold particles to label FITC liposomes and monitor their endocytosis on human coronary artery endothelial cells (HCAECs) in vitro. We were able to study the internalization process of gold-coupled liposomes on endothelial cells, by using AFM. We found that the gold-liposomes attached to the HCAEC cell membrane during the first 15-30 min of incubation, liposome cell internalization occurred from 30 to 60 min, and most of the gold-labeled liposomes had invaginated after 2 hr of incubation. Liposomal uptake took place most commonly at the periphery of the nuclear zone. Dynasore monohydrate, an inhibitor of endocytosis, obstructed the internalization of the gold-liposomes. This study showed the versatility of the AFM technique, combined with fluorescent microscopy, for investigating liposome uptake by endothelial cells. The 90 nm colloidal gold nanoparticles proved to be a noninvasive contrast agent that efficiently improves AFM imaging during the investigation of biological nanoprocesses.
K‐region oxides and imines of benzo[<i>b</i>]phenanthro[2,3‐<i>d</i>]thiophene and benzo[<i>b</i>]phenanthro[3,2‐<i>d</i>]thiophene (1993)

Abstract The syntheses of the K‐oxides and K‐imine derivatives of benzo[ b ]phenanthro[2,3‐ d ]thiophene and benzo‐[ b ]phenanthro[3,2‐ d ]thiophene are described. The parent hydrocarbons 1 and 2 were oxidized with osmium tetroxide and sodium metaperiodate, and the dialdehydes 12 and 18 so formed, cyclized to the corresponding epoxides 1a,12b‐dihydrobenz[ b ]oxireno[9,10]phenanthro[2,3‐ d ]thiophene ( 7 ) and 1a,12b‐dihydrobenz‐[ b ]oxireno[9,10]phenanthro[3,2‐ d ]thiophene ( 13 ). Reaction of the oxiranes with sodium azide gave mixtures of azido‐alcohols that, in turn, were transformed to the thiaarene imines 1a,12b‐dihydro‐1 H ‐benz[ b ]azirino‐[9,10]phenanthro[2,3‐ d ]thiophene ( 8 ) and 1a,12b‐dihydro‐1 H ‐benz[ b ]azirino[9,10]phenanthro[3,2‐ d ]thiophene ( 14 ), respectively, with the aid of tri‐ n ‐butylphosphine.
Peptide Analysis Protocols (1994)

Gel-Filtration Chromatography Daniel M. Bollag. Ion-Exchange Chromatography Daniel M. Bollag. Reversed-Phase HPLC: Analytical Procedure Udo Nirenberg. Reversed-Phase High-Performance Liquid Chromatography: A Semipreparative Methodology Michael E. Byrnes. Applications of Strong Cation-Exchange (SCX)-HPLC in Synthetic Peptide Analysis Dan L. Crimmins. Principles and Practice of Peptide Analysis with Capillary Zone Electrophoresis Thomas E. Wheat. Fast Atom Bombardment Mass Spectrometric Characterization of Peptides P. R. Das and B. N. Pramanik. Sequence Analysis of Peptide Resins from Boc/Benzyl Solid- Phase Synthesis Jan Pohl. NMR Spectroscopy of Peptides and Proteins Mark G. Hinds and Raymond S. Norton. Techniques for Conjugation of Synthetic Peptides to Carrier Molecules J. Mark Carter. Epitope Prediction Methods J. Mark Carter. Epitope Mapping of a Protein Using the Geysen (PEPSCAN) Procedure J. Mark Carter. Analysis of Proteinase Specificity by Studies of Peptide Substrates:The Use of UV and Fluorescence Spectroscopy to Quantitate Rates of Enzymatic Cleavage Ben M. Dunn Paula E. Scarborough Ruth Davenport and Wieslaw Swietnicki. Synthesis of Recombinant Peptides Gino Van Heeke Jay S. Stout and Fred W. Wagner. De Novo Design of Proteins: Template-Assembled Synthetic Proteins (TASP) Gabriele Tuchscherer Verena Steiner Karl- Heinz Altmann and Manfred Mutter. Chemical Synthesis of the Aspartic Proteinase from Human Immunodeficiency Virus (HIV) Paul D. Hoeprich Jr. Multiple and Combinatorial Peptide Synthesis: Chemical Development and Biological Applications Philip C. Andrews Daniele M. Leonard Wayne L. Cody and Tomi K. Sawyer. Index.
Expression of pericyte, mesangium and muscle markers in malignant rhabdoid tumor cell lines: Differentiation‐induction using 5‐azacytidine (2003)

Malignant rhabdoid tumor (MRT) has been considered to have multiphenotypic diversity characteristics. Some MRTs exhibit a neural phenotype. However, it is still unclear whether MRT cells can display a skeletal muscle, smooth muscle or smooth muscle-like cell phenotype, like those of pericytes and mesangial cells. To determine if MRTs exhibit skeletal muscle cell or smooth muscle-like cell phenotypes, six MRT cell lines (TM87-16, STM91-01, TTC549, TTC642, YAM-RTK1 and TTC1240) were examined for markers of skeletal muscle (MyoD, myogenin, myf-5, myf-6, acetylcholine receptor-alpha, -beta and -gamma), smooth muscle (alpha-smooth muscle actin, SM-1 and SM22), and smooth muscle-like cells, such as pericytes (angiopoietin-1 and -2) and mesangial cells (megsin), using conventional RT-PCR, semi-quantitative PCR, western blotting and immunocytochemistry before and after differentiation-induction with 5-azacytidine. alpha-Smooth muscle actin and SM22 were detected in all six MRT cell lines, while MyoD and myf-5, crucial markers for skeletal myogenic determination, were not. The TM87-16 cell line expressed SM-1 and angiopoietin-1. TTC1240 also expressed angiopoietin-1. Interestingly, STM91-01 expressed megsin, a novel marker for mesangial cells, in addition to angiopoietin-1. Our results indicated that some MRTs exhibited smooth muscle and/or smooth muscle-like cell phenotypes and some renal MRTs might be of mesangial origin. Recently, smooth muscle and also smooth muscle-like cells have been considered to be of neuroectodermal origin. MRT can thus considered to belong to the category of primitive neuroectodermal tumors (PNETs) in the broad sense.
Untersuchung der binären Systeme von Alkoxy‐silan—Alkohol und Phenoxy‐silan—Phenol (1970)

Abstract Aus den Ergebnissen von DK‐ und IR‐Messungen wird auf eine Komplexbildung von Alkyl‐alkoxy‐silan und Alkohol im Molverhältnis 2:1 geschlossen. Bei monofunktionellen Gemischen kann die Komplexbildung nur aus dem IR‐Spektrum nachgewiesen werden. Aus der Verschiebung der v OH‐Schwingung wird die Stärke der Wasserstoffbrückenbindung zu 4,29 kcal/mol errechnet. Die Neigung zur Bildung akzeptorartiger Komplexverbindungen steigt in Richtung der Monofunktion. Das Siliciumatom befindet sich in den Assoziationskomplexen im sp 3 d‐hybridisiertem Zustande. Aus den Ergebnissen der DK‐ und IR‐Messungen ist zu ersehen, daß die Komplexbildung im Alkyl‐phenyoxy‐silan und Phenol bei einem Molverhältnis 1:1 am stärksten ist.
MPS 13-05 LOCOMOTOR ACTIVITY IN CONGENIC SHRSPwch1.0 RATS IS DERIVED FROM THE INCREASE IN SYSTOLIC ARTERIAL PRESSURE BUT NOT THE SYSTOLIC ARTERIAL PRESSURE LEVEL ITSELF (2016)

Objective: Congenic rats (SHRSPwch1.0) were produced from stroke-prone SHR/Izumo rats (SHRSP/Izm) and Wistar-Kyoto rat/Izumo rats (WKY/Izm). We previously found that the systolic arterial pressure (SAP) and heart rate (HR) of the SHRSPwch1.0 were lower than those of the SHRSP/Izm. However, the SHRSPwch1.0 exhibited greater locomotor activity (ACT) than the SHRSP/Izm. The purpose of this study was to investigate the ACT characteristics of SHRSPwch1.0 in a time-series analysis. Design and Method: We used ten mature male SHRSPwch1.0 and ten male age-matched SHRSP/Izm as controls. They were kept under a 12-hour light and dark cycle in identical conditions. The rats’ SAP, HR, and ACT were monitored using radio telemetry, and their ACT was analyzed using the maximum entropy method to enable a spectral analysis of ACT. Results: All of the examined ACT values (24-hour, dark period, and light period) were greater in the SHRSPwch1.0 than in the SHRSP/Izm. A time-series analysis of ACT in the SHRSPwch1.0 revealed both circadian (23.379 hours) and ultradian variations (16.197 hours and 12.184 hours). On the other hand, the ACT of the SHRSP/Izm exhibited circadian variations (23.190 hours) and an almost imperceptible ultradian rhythm. The power spectral density of the ACT spectrum was greater in the SHRSPwch1.0 than in the SHRSP/Izm. A stronger correlation was noted between ACT and SAP in the SHRSPwch1.0 than in the SHRSP/Izm. In addition, the ACT response to increases in SAP was stronger in the SHRSPwch1.0 than in the SHRSP/Izm. However, the ACT response to increases in HR did not differ between the SHRSPwch1.0 and SHRSP/Izm. Conclusions: These findings suggest that ACT continues for longer in SHRSPwch1.0 than in SHRSP/Izm. In SHRSPwch1.0, ACT is strongly correlated with the magnitude of increases in SAP, but not the SAP level itself or the size of increases in HR.
ACCURATE MASS ANALYSIS OF GLYCOPROTEIN VARIANTS BY ELECTROSPRAY IONISATION, ORTHOGONAL ACCELERATION TIME-OF-FLIGHT MASS SPECTROMETRY AND MAXIMUM ENTROPY (2004)

ACCURATE MASS ANALYSIS OF GLYCOPROTEIN VARIANTS BY ELECTROSPRAY IONISATION, ORTHOGONAL ACCELERATION TIME-OF-FLIGHT MASS SPECTROMETRY AND MAXIMUM ENTROPY
The Influence of VIM Crucible Composition, Vacuum ARC Remelting, and Electroslag Remelting on the Non-Metallic Inclusion Content of MERL 76 (1980)

A program was conducted to determine the effect of selected melt variables on the oxide inclusion content of MERL 76 ingot material. Melt techniques investigated included vacuum induction (VIM), vacuum arc remelting (VAR, and electroslag remelting (ESR). Results of the VIM study revealed that magnesia crucibles produced ingot material having the lowest oxide content whereas zirconia crucibles resulted in the highest oxide content. Vacuum arc remelting was shown to have little or no effect on reducing the oxide content of the starting ingot, whereas ESR was shown to significantly reduce oxide content. A technique for quantitatively assessing oxide content of an ingot was also developed and demonstrated.
End-user recommendations of mobile services (2012)

Tens of thousands of mobile services and applications are available to end-users through app stores and developer web sites. From these sources, the end-users need to find and select the ones that best suit their needs. Recommendation in different forms and through different mediums from other end-users can act as an additional, and more specific, channel for this purpose. In this paper we present the results of an interview study in which the aim was to explore the motivations and means of recommending mobile services to potential users in people's social circles. We found that recommendations are largely made face-to-face, but the emerging role of social media in recommending services offers possibilities for wide service distribution. Our findings highlight the importance of specific and personal recommendations, pointing to a consideration in incorporating social media tools in app stores and markets. In addition, users should be able to share rich experiences in such tools and provide context-sensitive recommendations based on their experiences.
Optimizing challenge in an educational game using large-scale design experiments (2013)

Online games can serve as research instruments to explore the effects of game design elements on motivation and learning. In our research, we manipulated the design of an online math game to investigate the effect of challenge on player motivation and learning. To test the \'1cInverted-U Hypothesis\'1d, which predicts that maximum game engagement will occur with moderate challenge, we produced two large-scale (10K and 70K subjects), multi-factor (2x3 and 2x9x8x4x25) online experiments. We found that, in almost all cases, subjects were more engaged and played longer when the game was easier, which seems to contradict the generality of the Inverted-U Hypothesis. Troublingly, we also found that the most engaging design conditions produced the slowest rates of learning. Based on our findings, we describe several design implications that may increase challenge-seeking in games, such as providing feedforward about the anticipated degree of challenge.
Estimating the Prevalence of Agitation in Community-Dwelling Persons With Alzheimer's Disease (2002)

To estimate the prevalence of, and develop norms for, significant agitation in community-dwelling persons with Alzheimer's disease (AD), the authors applied three different criteria to persons with AD (n=235) and normal elderly control subjects (NEC; n=64). The criteria were used to identify the minimum total score on the Cohen-Mansfield Agitation Inventory (CMAI) that represents significant or “excessive” agitation and to estimate its prevalence. The “ultraliberal” criterion resulted in 99.1% of persons with AD and 56.6% of NEC being classified as “excessively” disturbed. The “liberal” and “conservative” criteria classified 66.7% and 68.2% of persons with AD, and no NEC, as “excessively” disturbed. The authors conclude that the best estimate of prevalence of excessive agitation in this population is 67.5%, and that individuals with CMAI scores of 0 to 14 probably should not be considered to have excessive agitation.
CLINICAL AND RADIOLOGICAL FINDINGS IN PATIENTS WITH GAS FORMING RENAL ABSCESS TREATED CONSERVATIVELY (1999)

We describe a unique entity within the spectrum of pyelonephritis. The clinical appearance of gas forming abscesses within the renal parenchyma without liquefaction in diabetic patients was remarkably benign compared to the radiographic appearance of the disease process. Conservative management with intravenous and oral antibiotics was successful in each patient, avoiding the need for invasive intervention.
Progesterone regulation of vascular thromboxane A<sub>2</sub>receptors in rhesus monkeys (2001)

We hypothesized that progesterone regulates thromboxane A(2) receptor (TxA(2)R) density in primate vascular muscle and that TxA(2)R density correlates with coronary reactivity in vivo and in vitro. Reactivity to serotonin + U-46619 was determined by angiography in surgically postmenopausal [ovariectomized (Ovx)] rhesus monkeys without progesterone replacement and after 2-wk progesterone treatment (1-2 ng/ml). In untreated Ovx animals, 100 micromol/l serotonin + 1 micromol/l U-46619 (syringe concentrations) provoked vasospasm-like constrictions in six of six monkeys; zero of six progesterone-treated monkeys developed vasospasms. Sustained Ca(2+) responses in vascular muscle cells isolated from Ovx coronaries (208 +/- 63% of basal 20 min after stimulation) treated with serotonin + U-46619 contrasted with transient Ca(2+) responses (143 +/- 18% of basal and decreasing 5 min after stimulation) in progesterone-treated monkeys. The maximum density of [1S-(1I,2J(5Z),3I(1E,3R*),4I)]-7-[3-(3-hydroxy-4-(4'-[(125)I]iodophenoxy)- 1-butenyl)-7-oxabicyclo[2.2.1]heptan-2-yl]-5-heptenoic acid ([(125)I]-BOP) binding was greater (P < 0.01) in carotid arteries and aortic membranes from Ovx (109 +/- 11 fmol/mg) compared with progesterone-treated (43 +/- 15 fmol/mg) monkeys. TxA(2)R immunolabeling revealed greater coronary TxA(2)R labeling in Ovx compared with progesterone-treated monkeys. The results suggest that progesterone can decrease arterial TxA(2)R in Ovx monkeys.
Nickel-Catalyzed Synthesis of Pyrazolo[3,4-<i>c</i>]pyridines via Alkyne Annulation (2008)

Reported is a reasonably efficient Ni-catalyzed synthesis of polysubstituted pyrazolo[3,4-c]pyridines by the annulation of alkynes with iodopyrazolocarboximines. The starting pyrazoles are readily prepared in two steps from protected 4-iodopyrazoles in 50-60% overall yield. Annulations using internal alkynes (R3 and R4 ∂ H) always proceed in higher yields but with lower regioselectivity (when R3 and R4 are different) than reactions using terminal alkynes. The tolerance of functional R1 and R2 substitutents was inadequately investigated. In one case, a Pd catalyst, PdCl2(PhCN)2, was successfully tested for the annulation.