gebhart/patrepeval huggingface dataset:
A cultured malignant B-1 line serves as a model for Richter's syndrome. (1994)

Human chronic lymphocytic leukemia (CLL) is a malignancy of B-1 cells characterized by the accumulation of mature appearing, long lived, slow growing B-1 cells in peripheral blood. CLL occasionally evolves into an aggressive large cell lymphoma termed Richter's syndrome. NZB mice can be used to model the early stage of CLL because aged NZB mice can spontaneously develop slow growing malignant B-1 cell clones. The malignant NZB B-1 clones fail to grow in culture and are typically carried in vivo as passaged lines. During serial passage, an aggressive lymphoma developed as a result of a continued transformation of the original B-1 clone, similar to the development of Richter's syndrome. An in vitro cell line was established from the aggressive lymphoma, which was stromal dependent and could rapidly metastasize when passaged into recipient animals. Analysis of adhesion molecules did not reveal any consistent characteristics that could account for the metastatic potential of the Richter's-like cells. In addition, the aggressive in vitro line had the identical heavy chain sequence as the slow growing NZB malignant B-1 clones. The in vitro and in vivo aggressive B-1 cells had very high levels of IL-10 message, and underwent more apoptosis in response to anti-IgM than did nonaggressive B-1 clones. Taking these characteristics together, we have composed a comprehensive animal model system for human CLL that includes both the aged NZB mice for the early stage and the recipients of the in vitro B-1 line for the late stage or Richter's syndrome. This model system can be used to study, not only the ontogeny and genetic linkage of CLL, but also the regulatory factors involved in transformation and growth both in vivo and in vitro.
Effects of Water Quality on Hypoxia in the Gulf of Mexico (1996)

In many aquatic systems, incidences of low oxygen (hypoxia) waters have been causally linked to increased nutrient concentrations. The Mississippi River accounts for approximately 80-90% of the freshwater inflow to the Gulf of Mexico; water quality in the Mississippi River should serve as a indicator of conditions in the Gulf. Near its mouth, the Mississippi River splits, with approximately one-third of its discharge diverted into the AtcbafaJaya River. A review of the literature (primarily peer reviewed journals and technical publications) suggests that the Mississippi River waters enriches the Gulf of Mexico and this enrichment has increased in recent years. However, 21 years of data collected and published by the United States Geological Survey indicates no change in total phosphorus. Nitrogen values for the Mississippi River have increased (concomitantly with a decline in nitrogen values in the Atchafalaya River). There is good evidence that hypoxic areas in the Gulf of Mexico have increased following the 1993 flooding of the centralmidwestern United States. The timing and intensity of river discharge into the Mississippi River apparently controls the duration and extent of hypoxia within the Gulf of Mexico.
Quasi-3D Strain Analysis in Equal Channel Angular Pressing (2004)

Although deformation features of ECAP have been widely studied and investigated through numerical approaches, experimental observations are very scarce in the literature. Therefore, in this paper, quasi‐3D strains in multi‐sectioned samples during one pass of ECAP are analyzed, for different friction conditions and material properties. From this study, it can be concluded that ECAP is a plane‐strain process and friction and material properties affect the strain distribution, strain homogeneity and also the work‐piece geometry. Furthermore, ECAP can be interpreted as a combination of shear and stretch.
Role of N- and C-terminal amino acids in antithrombin binding to pentasaccharide. (1994)

We have used a monoclonal antibody-based binding procedure to determine the dissociation constants of the interactions between the essential antithrombin-binding pentasaccharide and a series of 13 distinct N- and C-terminal antithrombin substitution mutation variants with defective binding interaction with heparin. The reduction in binding affinity of the pentasaccharide with the N-terminal variants (with substitution mutations Pro-41-->Leu, Arg-47-->Cys and His, Leu-99-->Val and Phe, Gln-118-->Pro, Arg-129-->Gln) compared to normal antithrombin, Kd 200 nM, ranged from 15-984-fold and was generally less than 150-fold. Reduced binding affinity is assumed to arise mostly by perturbation, direct or indirect, of the initial contact of pentasaccharide with basic residues of antithrombin. Surprisingly, the binding interaction of the pentasaccharide with the C-terminal variants (with substitution mutations in or near strand 1C/4B, Phe-402-->Leu, Cys, and Ser, Ala-404-->Thr, Pro-407-->Thr, Pro-429-->Leu) was more uniformly and yet substantially (135-482-fold) decreased, despite the spatial separation between the site of mutation and the proposed primary contact site of the pentasaccharide. These results demonstrate that strand 1C/4B region integrity is required for optimum interaction with the pentasaccharide, suggesting its involvement in transmission of the induced conformation change required for high affinity binding.
Detecting DNA damage (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDETECTING DNA DAMAGEJean Cadet and Michael WeinfeldCite this: Anal. Chem. 1993, 65, 15, 675A–682APublication Date (Print):August 1, 1993Publication History Published online31 May 2012Published inissue 1 August 1993https://pubs.acs.org/doi/10.1021/ac00063a724https://doi.org/10.1021/ac00063a724research-articleACS PublicationsRequest reuse permissionsArticle Views284Altmetric-Citations63LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Auflösung Linearer Algebraischer Gleichungssysteme (1979)

Bei der Einteilung von Kontinua in finite Elemente kommt der Auflösung des dabei anfallenden Gleichungssystems (2.1) $$kv = f$$ (K ist symmetrisch und positiv definiert) eine Schlüsselstellung zu, die vom Umfang der zu behandelnden Probleme und der zur Verfügung stehenden Rechenanlage abhängig ist.
Bronchial responsiveness to methacholine and adenosine 5′‐monophosphate in atopic and non‐atopic preschool children with recurrent wheezing (2006)

While a majority of both atopic and non-atopic preschool wheezers were hyper-responsive to methacholine, atopic subjects were more hyper-responsive to AMP than non-atopic subjects. These findings suggest that atopic and non-atopic wheeze in preschool children are related to distinctive pathophysiologic pathways.
Sound absorption in a mixture of O2 and N2 (1974)

The attenuation of a 1-MHz ultrasonic signal has been measured in 72% N2/28% O2 at temperatures of 300°, 500°, and 700 °K and pressures from 1 atm to 1/100 atm. The measured attenuation was corrected for classical effects to obtain the attenuation due to rotational relaxation. In addition, the total absorption has been compared to computed values based on a linear combination of viscous, thermal conduction, diffusion, and relaxation to check the constants used for each of these terms. [Work supported by Army Research Office, Durham, North Carolina.]
The Natural History and Molecular Heterogeneity of HIV-Associated Primary Malignant Lymphomatous Effusions (1996)

Primary malignant lymphomatous effusions arising in individuals infected with the human immunodeficiency virus, type 1 (HIV-1) represent a rare subset of HIV-associated lymphomas. Previous studies have demonstrated that the malignant cells are monoclonal (as defined by rearrangement of the immunoglobulin gene), express cell surface CD38, and are infected with Epstein-Barr virus (EBV) and human herpes virus, type 8 (HHV-8). Despite these detailed molecular and immunophenotypic studies, clinical information on this disease entity is scant, prompting us to review the clinical features of eight cases seen at our institutions. All eight patients had total peripheral CD4+ lymphocytes < 200/microliter and presented with complaints related to body cavity distension. Routine laboratory values were nondiagnostic and yielded no prognostic information. Only two patients could tolerate and thus received chemotherapy with no obvious impact on their clinical course. The mean overall survival after diagnosis was 60 days (range 6-166 days). Four patients were examined at autopsy. The primary malignant lymphomatous effusion either was the immediate cause of death or contributed significantly to the death of only two. All four patients examined post mortem, however, had lymphomatous infiltration of serosal surfaces adjacent to the site of the primary malignant effusion. Molecular and immunologic studies performed on the malignant cells and effusion fluids revealed universal expression of cell surface CD38 and the presence of HHV-8 gene sequences, but in contrast with previous studies, only four had rearranged immunoglobulin genes or EBV present: IL-6 and IL-10 levels in the malignant effusion fluids were markedly elevated. In summary, this rare subset of HIV-associated lymphomas in our eight patients arose late in the course of HIV-associated disease, had a rapid clinical course, and was molecularly heterogeneous. A pathogenetic role for HHV-8 alone in this disease process is strengthened by our observation of four cases lacking EBV but containing HHV-8.
Activation of descending pain-facilitatory pathways from the rostral ventromedial medulla by cholecystokinin elicits release of prostaglandin-E2 in the spinal cord (2011)

Cholecystokinin (CCK) has been suggested to be both pro-nociceptive and "anti-opioid" by actions on pain-modulatory cells within the rostral ventromedial medulla (RVM). One consequence of activation of RVM CCK₂ receptors may be enhanced spinal nociceptive transmission; but how this might occur, especially in states of pathological pain, is unknown. Here, in vivo microdialysis was used to demonstrate that levels of RVM CCK increased by approximately 2-fold after ligation of L₅/L₆ spinal nerves (SNL). Microinjection of CCK into the RVM of naïve rats elicited hypersensitivity to tactile stimulation of the hindpaw. In addition, RVM CCK elicited a time-related increase in (prostaglandin-E₂) PGE₂ measured in cerebrospinal fluid from the lumbar spinal cord. The peak increase in spinal PGE₂ was approximately 5-fold and was observed at approximately 80 minutes post-RVM CCK, a time coincident with maximal RVM CCK-induced mechanical hypersensitivity. Spinal administration of naproxen, a nonselective COX-inhibitor, significantly attenuated RVM CCK-induced hindpaw tactile hypersensitivity. RVM-CCK also resulted in a 2-fold increase in spinal 5-hydroxyindoleacetic acid (5-HIAA), a 5-hydoxytryptophan (5-HT) metabolite, as compared with controls, and mechanical hypersensitivity that was attenuated by spinal application of ondansetron, a 5-HT₃ antagonist. The present studies suggest that chronic nerve injury can result in activation of descending facilitatory mechanisms that may promote hyperalgesia via ultimate release of PGE₂ and 5-HT in the spinal cord.
The Performance of Zirconium Alloys in Sulfide Solutions (1982)

Zirconium Grades R 60702, R 60704, and R 60705 were investigated for deep sour gas well applications. Test methods include autoclave, constant strain, sustained load, and constant strain rate techniques. Test results indicate that these alloys have excellent resistance to general corrosion and to stress corrosion cracking in sulfide environments. No cracking was detected in all grades of zirconium in 5% NaCl + 0.5% CH3COOH + saturated H2S at room and boiling temperatures. Hydrogen sulfide seems to play a neutral role in the corrosion reactions of zirconium because of zirconium's low affinity for sulfur.
Implantable semiconductor imaging devices for in vivo optical imaging of brain (2015)

We present ultra-small and mass-producible semiconductor imaging devices for functional imaging of brain. Proof-of-concept experiments including blood-flow estimation and fluorescence imaging of the brain demonstrated promise for use in brain research.
Correlative Fluorescence and Electron Microscopy of Graphene-Enclosed Whole Cells for High Resolution Analysis of Cellular Proteins (2019)

Membrane proteins are the main targets of medical treatments, but their expression can vary in between different cells as well as between different areas within a cell This heterogeneity represents a major obstacle in tumor therapy since it can give rise to resistant tumor cells Yet, techniques to analyze the distribution of membrane proteins in their native environment remain limited Electron microscopy (EM) provides the necessary resolution but during conventional sample preparation, the information about the spatial arrangement within the context of the whole cell is usually lost and data is typically obtained from a few cellular regions only. Liquid-phase EM offers the opportunity to study series of whole hydrated cells but requires special experimental set-ups
Binding, uptake, and localization of surfactant protein B in isolated rat alveolar type II cells (1992)

This study reports the ability of rat alveolar type II cells to internalize mature bovine surfactant protein B (SP-B) in vitro. Isolated type II cells were incubated with labeled SP-B, and binding and internalization were studied biochemically and morphologically. Biochemical analyses demonstrated a time-dependent association of 125I-labeled SP-B with type II cells; binding steadily increased through 4 h and then remained constant through 20 h of incubation. The association of [3H]SP-B with type II cells was characterized via light and electron microscopic autoradiography. Significant quantities of [3H]SP-B were found at the plasma membrane, in the endocytic pathway, and in lamellar bodies. The pathway of SP-B internalization was not altered by the presence of whole rat surfactant; however, the quantity of SP-B internalized into lamellar bodies was increased. 3[H]SP-B was not associated with coated pits and colocalized with horseradish peroxidase (HRP), consistent with receptor-independent internalization. Cell-associated SP-B was not degraded and was detected in lamellar bodies undergoing exocytosis. These results suggest that SP-B may follow a recycling pathway similar to that previously reported for surfactant phospholipids.
Towards bounded wait-free PASIS (2007)

The PASIS read/write protocol implements a Byzantine fault-tolerant erasure-coded atomic register. The prototype PASIS storage system implementation provides excellent best-case performance. Writes require two round trips and contention- and failure-free reads require one. Unfortunately, even though writes and reads are wait-free in PASIS, Byzantine components can induce correct clients to perform an unbounded amount of work. In this extended abstract, we enumerate the avenues by which Byzantine servers and clients can induce correct clients to perform an unbounded amount of work in PASIS. We sketch extensions to the PASIS protocol and Lazy Verification that bound the amount of work Byzantine components can induce correct clients to perform. We believe that the extensions provide bounded wait-free reads and writes. We also believe that an implementation that incorporates these extensions will preserve the excellent best-case performance of the original PASIS prototype.
Evaluation of Proctophyllodes huitzilopochtlii on feathers from Anna’s (Calypte anna) and Black-chinned (Archilochus alexandri) Hummingbirds: Prevalence assessment and imaging analysis using light and tabletop scanning electron microscopy (2018)

Proctophyllodes huitzilopochtlii Atyeo & Braasch 1966 (Acariformes: Astigmata: Proctophyllodidae), a feather mite, was found on feathers collected from five hummingbird species in California. This mite has not been previously documented on feathers from Anna's (Calypte anna [Lesson 1829]) or Black-chinned (Archilochus alexandri [Bourcier & Mulsant 1846]) Hummingbirds. A total of 753 hummingbirds were evaluated for the presence of mites by species (Allen's n = 112; Anna's n = 500; Black-chinned n = 122; Rufous n = 18; Calliope n = 1), sex (males n = 421; females n = 329; 3 unidentified), and age (juvenile n = 199; after-hatch-year n = 549; 5 unidentified). Of these 753 hummingbirds evaluated, mites were present on the rectrices of 40.9% of the birds. Significantly more Anna's Hummingbirds were positive for rectricial mites (59.2%) compared with 8.2% of Black-chinned, 0.9% of Allen's, 5.6% of Rufous Hummingbirds, and 0% for Calliope (p-value < 0.0001). Across all hummingbird species, male hummingbirds (44.9%) had a higher prevalence of rectricial mites compared to female hummingbirds (36.2%; p-value = 0.004), while juvenile hummingbirds (46.2%) had a non-significantly higher prevalence compared to after-hatch-year hummingbirds (39.0%; p-value = 0.089). On average, the percentage of the long axis of the rachis occupied by mites for the outer rectrices (R4 and R5) was 19%, compared to 11% for inner rectrices (R1 and R2), a significant difference (p-value = <0.0001). There was a marginal lack of significance for symmetrical distribution of tail mites with the mean left side percentage of long axis of the rachis occupied by mites being 16% and very close to the mean right side score of 18% (p-value = 0.003). The identification of the feather mite species was based on light microscopic morphometry, and mite distribution on feathers was further evaluated using tabletop scanning electron microscopy (TSEM). The hummingbird-feather mite relationship is not well understood, but the specialized TSEM technique may be especially useful in examining natural positioning and developmental aspects of the mites since it allows in situ feather examination of live mites.
Sulfate as a Synergistic Anion Facilitating Iron Binding by the Bacterial Transferrin FbpA:  The Origins and Effects of Anion Promiscuity (2007)

The ferric binding protein, FbpA, has been demonstrated to facilitate the transport of naked Fe3+ across the periplasmic space of several Gram-negative bacteria. The sequestration of iron by FbpA is facilitated by the presence of a synergistic anion, such as phosphate or sulfate. Here we report the sequestration of Fe3+ by FbpA in the presence of sulfate, at an assumed periplasmic pH of 6.5 to form FeFbpA-SO4 with K'(eff) = 1.7 x 10(16) M(-1) (at 20 degrees C, 50 mM MES, 200 mM KCl). The iron affinity of the FeFbpA-SO4 protein assembly is 2 orders of magnitude lower than when bound with phosphate and is the lowest of any of the FeFbpA-X assemblies yet reported. Iron reduction at the cytosolic membrane receptor may be an essential aspect of the periplasmic iron-transport process, and with an E(1/2) of -158 mV (NHE), FeFbpA-SO4 is the most easily reduced of all FeFbpA-X assemblies yet studied. The variation of FeFbpA-X assembly stability (K'(eff)) and ease of reduction (E(1/2)) with differing synergistic anions X(n-) are correlated over a range of 14 kJ, suggesting that the variations in redox potentials are due to stabilization of Fe3+ in FeFbpA-X by X(n-). Anion promiscuity of FbpA in the diverse composition of the periplasmic space is illustrated by the ex vivo exchange kinetics of FeFbpA-SO4 with phosphate and arsenate, where first-order kinetics with respect to FeFbpA-SO4 (k = 30 s(-1)) are observed at pH 6.5, independent of entering anion concentration and identity. Anion lability and influence on the iron affinity and reduction potential for FeFbpA-X support the hypothesis that synergistic anion exchange may be an important regulator in iron delivery to the cytosol. This structural and thermodynamic analysis of anion binding in FeFbpA-X provides additional insight into anion promiscuity and importance.
Exploiting frame length of 802.15.4g signals for wake-up control in sensor networks (2015)

Sensor networks are playing more and more important roles, e.g., accurately monitoring the generation and consumption of electric power for the purpose of smart grid. In these applications, sensor nodes, working with battery, should be put into sleep in the idle state to prolong network lifetime and be activated on demand to ensure real-time response. State-of-the-art schemes merely exploiting duty-cycling cannot simultaneously satisfy these two requirements. In this paper, we exploit frame length of 802.15.4g signals for the wake-up control of sensor nodes. A wake-up ID is modulated onto frame lengths of consecutive signals defined by the mode switch mechanism, transmitted by a sensor node using the standard 802.15.4g protocol, and detected by a non-802.15.4g, low-power wake-up receiver. A prototype wake-up receiver is implemented by using FPGA. It consists of two-stage wake-up control in the sense that duty-cycling is also exploited in the detection of wake-up signals. The proposed scheme achieves a better tradeoff between power-consumption and wake-up latency, compared with the conventional duty-cycling schemes. The experimental evaluation confirms that the wake-up control meets the sensitivity requirement of data communications.
High speed pulsed electrical spin injection in spin-light emitting diode (2009)

We demonstrate high speed pulsed electrical spin injection from a CoFeB/MgO spin injector into a AlGaAs/GaAs semiconductor light emitting diode. Under pulsed electrical excitation, time-resolved electroluminescence on nanosecond time scale exhibits a plateau of circular polarization degree as high as 15% under a 0.8 T magnetic field. It follows an initial decay that could be due to electron spin-relaxation process in the quantum well embedded in the intrinsic region of the diode. The temporal buildup of the electronic spin polarization degree in the quantum well is much faster than the rise time of electroluminescence intensity.
Thiophene-2-aryl-2<i>H</i>-benzotriazole-thiophene Oligomers with Adjustable Electronic Properties (2011)

The synthesis and the optical and electrochemical properties of 2-aryl-2H-benzotriazole based thiophene oligomers with chemical modifications either on the phenyl side group or on the backbone are presented. All data, supported by DFT calculations, show that modification on the backbone has a major impact on the electronic properties while the side groups can fine-tune the electronic properties. In addition, one compound exhibits a thermotropic mesophase.