gebhart/patrepeval huggingface dataset:
Ceramide 1-Phosphate Mediates Endothelial Cell Invasion via the Annexin a2-p11 Heterotetrameric Protein Complex (2013)

The bioactive sphingolipid, ceramide 1-phosphate (C-1-P), has been implicated as an extracellular chemotactic agent directing cellular migration in hematopoietic stem/progenitor cells and macrophages. However, interacting proteins that could mediate these actions of C-1-P have, thus far, eluded identification. We have now identified and characterized interactions between ceramide 1-phosphate and the annexin a2-p11 heterotetramer constituents. This C-1-P-receptor complex is capable of facilitating cellular invasion. Herein, we demonstrate in both coronary artery macrovascular endothelial cells and retinal microvascular endothelial cells that C-1-P induces invasion through an extracellular matrix barrier. By employing surface plasmon resonance, lipid-binding ELISA, and mass spectrometry technologies, we have demonstrated that the heterotetramer constituents bind to C-1-P. Although the annexin a2-p11 heterotetramer constituents do not bind the lipid C-1-P exclusively, other structurally similar lipids, such as phosphatidylserine, sphingosine 1-phosphate, and phosphatidic acid, could not elicit the potent chemotactic stimulation observed with C-1-P. Further, we show that siRNA-mediated knockdown of either annexin a2 or p11 protein significantly inhibits C-1-P-directed invasion, indicating that the heterotetrameric complex is required for C-1-P-mediated chemotaxis. These results imply that extracellular C-1-P, acting through the extracellular annexin a2-p11 heterotetrameric protein, can mediate vascular endothelial cell invasion.
Circadian Rhythms and Photoperiodism in Plants (1965)

1),Oedogonium,Pilobolus (3,19), (21),.
Production and Metabolism of Serotonin (5-HT) by the Human Adrenal Cortex: Paracrine Stimulation of Aldosterone Secretion by 5-HT (2001)

In the human adrenal cortex, serotonin (5-HT) is contained in mast-like cells, and we have shown that 5-HT stimulates aldosterone secretion, suggesting that 5-HT may control glomerulosa cells through a paracrine mechanism. Concurrently, the presence of 5-hydroxyindolacetic acid in human adrenocortical extracts indicates that 5-HT may be metabolized after local release by mast cells. The aim of the present study was to investigate in vitro the production and metabolism of 5-HT by the human adrenal cortex. Perifused adrenal slices released spontaneously detectable amounts of 5-HT (0.74 +/- 0.38 fmol/mg wet tissue.min). The mast cell-depleting drug compound 48/80 induced a burst of 5-HT secretion followed by a gradual increase in aldosterone production. Administration of the specific 5-HT(4) receptor antagonist GR 113808 (10(-6) M) did not affect compound 48/80-induced 5-HT release but abolished the stimulatory effect of compound 48/80 on aldosterone secretion, indicating that 5-HT released locally is responsible for a paracrine control of steroidogenesis. Incubation of cells from the human adrenal cortex with 5-HT (10(-5) M) provoked the formation of the 5-HT metabolite 5-hydroxytryptophol. The type A monoamine oxidase (MAO) inhibitor clorgyline (10(-6) M) suppressed the metabolism of 5-HT into 5-hydroxytryptophol. Immunocytochemical staining of cultured cells revealed the presence of a subpopulation of MAO-A-positive cells. Double labeling with an antiserum against chromogranin A showed that MAO-A was actually contained in chromaffin cells. Similarly, immunohistochemical staining of adrenal slices showed that MAO-A was expressed in chromaffin cells located both in the medulla and in intracortical rays. In conclusion, the present study shows that, in the human adrenal cortex, 5-HT, released by mast-cells, may stimulate aldosterone secretion in a paracrine manner. Our data also indicate that 5-HT is metabolized by MAO-A located in intracortical chromaffin cells.
New Regenerating Codes over Binary Cyclic Codes (2019)

Regenerating code is a class of erasure codes designed for distributed storage systems that can achieve optimal tradeoff between storage and repair bandwidth. Most existing constructions of regenerating codes are based on a finite field with large enough size. Recently, a new construction of regenerating codes over a binary cyclic code was proposed. It was shown that the new construction has lower computational complexity than the construction based on finite fields. This paper generalizes the construction by designing regenerating codes with binary cyclic codes that support more parameters. We show that the proposed coding method can achieve the fundamental tradeoff curve between the storage and repair bandwidth. We also give an example that an existing construction of regenerating codes can be transformed to a regenerating code over a binary cyclic code with less computational complexity. Furthermore, the proposed coding framework has more design space for exact repair constructions of minimum storage regenerating codes.
Unusual Solid State Properties of Transition Metal Complexes of Sulphur-Donor Ligands (1996)

Abstract Metal complexes of 1,2 dithiolene ligands are known to exhibit unusual solid state properties. The properties of the Mercury complexes of the new type of 1,2 dithiolene ligand which includes the TTF molecule framework is described. The structure and magnetic properties of the ferromagnet NH4[Ni(mnt)2].H2O are discussed.
Polysulfones for conservation in the ethylene polymer industry. Progress report No. 8, April-June 1980. [. gamma. radiation, catalysis] (1980)

Ethylene SO/sub 2/ polysulfone copolymers were prepared by both cobalt-60 gamma irradiation and chemical catalysis as a means of incorporating a polluting waste material such as SO/sub 2/ into useful ethylene polymers and also for the purpose of conserving ethylene feedstock. In addition, ethylene-SO/sub 2/ copolymer was prepared with monomeric acrylates and vinyl acetate to produce a modified material. Discs of modified and unmodified ethylene-SO/sub 2/ copolymer were pressure molded.
Gadolinium-Enhanced Elliptically Reordered Three-Dimensional MR Angiography in the Assessment of Hand Vascularization Before Radial Artery Harvest for Coronary Artery Bypass Grafting: First Experience (2001)

Contrast-enhanced MRA displays vascularization of the hand in detail and supplies ultrasound flow measurements for radial artery harvest, with high-resolution angiographic data about possible vessel variations and the presence of anastomoses between the radial or ulnar artery supply.
Re-evaluating the Gibbs Analysis of Surface Tension at the Air/Water Interface (2009)

Molecular areas of soluble films at the air/water interface have traditionally been calculated by applying the Gibbs equation to the steep linear decline in surface tension as the bulk concentration increases. This approach presupposes that the interface is saturated in the "Gibbs region," thereby allowing a single unique area to be calculated. We show that the areas derived by the Gibbs equation (typically 50-60 A(2)/molecule) are much too large to account for the abrupt surface tension decline. Moreover, a surface tension/concentration plot was observed for a system where micelle formation does not interfere with the Gibbs region. Nonetheless, the surface tension plot leveled off, ostensibly owing to saturation, when the Gibbs approach predicted a continued linear decline, proving that the interface in the Gibbs region is not saturated as generally assumed. This conclusion means that the hundreds of published molecular areas obtained by the Gibbs approach should be reconsidered.
A single nucleotide insertion in the canine interleukin-2 receptor gamma chain results in X-linked severe combined immunodeficiency disease (1995)

The immunologic and genetic analysis of a 14-week-old-male cardigan Welsh corgi puppy that presented with failure to thrive, diarrhea, and intermittent vomiting are described. The lack of palpable lymph nodes, the premature death of a male sibling, and similar clinical signs in a male cousin suggested that a primary immunodeficiency disease might be responsible for his poor clinical condition. Quantitation of serum immunoglobulins revealed low concentrations of IgG and undetectable IgA, yet normal concentrations of IgM. A complete blood cell count showed a slight anemia and lymphopenia. Although the peripheral blood contained a normal percentage of T cells, with an increased CD4:CD8 ratio, they were unable to proliferate in response to phytohemagglutinin (PHA) and/or interleukin 2 (IL-2). Furthermore, following PHA activation, the peripheral blood lymphocytes (PBL) demonstrated a nearly complete lack of IL-2 binding. All of these laboratory findings were identical with our previous findings from dogs with X-linked severe combined immunodeficiency (XSCID) that is due to a mutation in their IL-2 receptor gamma (IL-2R gamma) chain. Examination of the corgi's IL-2R gamma cDNA revealed an insertion of a cytosine following nucleotide 582, resulting in a premature stop codon prior to the transmembrane domain. The insertion also created an EcoO109 restriction enzyme site that enabled us to detect the mutation in the patient's genomic DNA. This new mutation in the IL-2R gamma chain discovered in a cardigan Welsh corgi puppy results in XSCID with similar immunologic abnormalities as observed in dogs with the same disease resulting from a different IL-2R gamma chain mutation.
Meropenem/colistin synergy testing for multidrug-resistant Acinetobacter baumannii strains by a two-dimensional gradient technique applicable in routine microbiology (2014)

The new test was shown to be diagnostically useful, easy to implement and less labour intensive than the classical method.
A picosecond-response photoconductive-sampling probe for digital circuit testing (2002)

Recent efforts in noninvasive high-frequency and high-resolution measurement techniques have led to the development of a number of photoconductive probes. In this paper the feasibility of using the fiber-coupled, micromachined probe for in-circuit testing and characterization is demonstrated by detecting waveforms at internal nodes of two different digital circuits. On the one hand, measurements have been carried out which reveal the performance of a circuit under standard operating conditions. In this case the measured electrical signals originate from an external source, i.e., an rf synthesizer. In a second application, femtosecond optical pulses have illuminated one of the transistors of a circuit to generate the signal that is measured. This second approach is used to emulate so-called single-event upsets (SEU), which are usually caused by cosmic particles in satellite-based electronic systems. These effects have a negative impact on the performance and reliability of these systems and therefore are a limiting factor for their commercial implementation. In the past, optical techniques to generate SEU effects have been successfully demonstrated for testing single devices. In contrast, the results presented here demonstrate the generation and detection of these effects inside a complex circuit environment. Thus, they may especially benefit the development of radiation-immune circuits.
Magnetic resonance molecular imaging of post‐stroke neuroinflammation with a P‐selectin targeted iron oxide nanoparticle (2009)

We have developed a magnetic resonance molecular imaging method using a novel iron-oxide contrast agent targeted towards P-selectin - MNP-PBP (magnetic nanoparticle-P-selectin binding peptide) - to image endothelial activation following cerebral ischemia/reperfusion. MNP-PBP consists of approximately 1000 PBP ligands (primary sequence: GSIQPRPQIHNDGDFEEIPEEYLQ GGSSLVSVLDLEPLDAAWL) conjugated to a 50 nm diameter aminated dextran iron oxide particle. In vitro P- and E-selectin binding was assessed by competition ELISA. Transient focal cerebral ischemia was induced in male C57/BL 6 mice followed by contrast injection (MNP-PBP; MNP-NH2; Feridex; MNP-PBP-FITC) at 24 h after reperfusion and T(2) magnetic resonance imaging at 9.4 T was performed. Infarction and microvasculature accumulation of contrast agent was assessed in coronal brain sections. MNP-PBP attenuated antibody binding to P-selectin by 34.8 +/- 1.7%. P-selectin was preferentially increased in the infarct hemisphere and MNP-PBP-FITC accumulation in the infarct hemisphere microvasculature was observed. Compared with the nontargeted iron oxide agents MNP-NH2 and Feridex, MNP-PBP showed a significantly greater T(2) effect within the infarction. MR imaging of P-selectin expression with a targeted iron oxide nanoparticle contrast agent may reveal early endothelial activation in stroke and other neuroinflammatory states.
Indirect Complementation of a Nontransforming Mutant of Polyoma Virus (1974)

Host range mutants of polyoma virus have been selected which make plaques on polyoma-transformed but not on normal 3T3 cells. These mutants are defective in transformation of rat and hamster cells and are unable to alter the cell surface of 3T3 cells with respect to lectin agglutinability (Benjamin 1970; Benjamin and Burger 1970).
Seam tracking control of robotic arc welding systems (1996)

On-line path correction for arc welding robots is described in the context of robot programming and sensor integration. Controller interface requirements are discussed in terms of the level of end-point control achievable using an external smart sensor interface. The self teaching ability that dynamic seam tracking creates for both robot and robot-positioner combinations is described. The resulting modular system provides a plug-in seam tracking capability to a variety of robot platforms.
Cyanobacterial peptides — Nature's own combinatorial biosynthesis (2006)

Cyanobacterial secondary metabolites have attracted increasing scientific interest due to bioactivity of many compounds in various test systems. Among the known structures, oligopeptides are often found with many congeners sharing conserved substructures, while being highly variable in others. A major part of known oligopeptides are of non-ribosomal origin and can be grouped into classes with conserved structural properties. Thus, the overall structural diversity of cyanobacterial oligopeptides only seemingly suggests an equally high diversity of biosynthetic pathways and respective genes. For each class of peptides, some of which have been found in all major branches of the cyanobacterial evolutionary tree, homologous synthetases and genes can be inferred. This implies that non-ribosomal peptide synthetase genes are a very ancient part of the cyanobacterial genome and presumably have evolved by recombination and duplication events to reach the present structural diversity of cyanobacterial oligopeptides. In addition, peptide synthetases would appear to be an essential part of the cyanobacterial evolution and physiology. The present review presents an overview of the biosynthesis of cyanobacterial peptides and corresponding gene clusters, the structural diversity of structural types and structural variations within peptide classes, and implications for the evolution and plasticity of biosynthetic genes and the potential function of cyanobacterial peptides.
Where Have All the Condoms Gone in Adolescent Programmes in the Democratic Republic of Congo (2006)

Decades of mismanagement, combined with the withdrawal of international cooperation and a protracted war, have seriously affected the health system in the Democratic Republic of Congo (DRC) and the health status of the population. As part of a Belgian development cooperation programme, we conducted a study in Kinshasa and Bukavu in April-May 2004 on how a rights-based approach could contribute to an effective and appropriate response to the sexual and reproductive health needs of Congolese adolescents. Access to condom information and supplies was studied in this context. A qualitative methodology was used, consisting of focus group discussions with adolescents and interviews with peer education programme officers. These programmes were supposed to be based on the recognition of adolescent sexual and reproductive health rights and the so-called ABC approach (abstinence, be faithful, condom use). We found, however, that sociocultural barriers and strict obedience to Vatican doctrine prevented adolescents from receiving accurate and comprehensive sexuality education and that condom supplies were blocked by peer education programme officers. The promotion of adolescent sexual and reproductive health rights is the responsibility of States, but the international community, non-governmental and faith-based organisations and donors play an essential role in assisting States in this respect and should never act in violation of adolescents' rights.
Generation of a novel stem cell factor-dependent mast cell progenitor. (1998)

Tissue mast cell development requires stem cell factor (SCF), whereas helminth-induced intestinal mucosal mast cell hyperplasia also requires T cell-derived factors such as IL-3. We generated progenitor mast cells (PrMC) from mouse bone marrow cells (BMC) in vitro with a triad of SCF, IL-6, and IL-10 that exhibit IL-3-mediated mitogenic and maturation responses. SCF/IL-6/IL-10 transiently elicited a cell subpopulation with the phenotype (c-kit(high)Thy-1(low)) of fetal blood promastocytes at 3 wk of culture that progressed within 1 wk to FcepsilonRI-bearing PrMC, designated PrMCTriad. PrMCTriad lacked mouse mast cell carboxypeptidase A (mMC-CPA) protein, required SCF for IL-3-driven thymidine incorporation, and responded to SCF plus IL-3 with strong mMc-CPA immunoreactivity, clarifying distinct sequential roles for SCF and IL-3 in mast cell development. PrMCTriad, arising from BMC through promastocytes, are metamastocytes that acquire microenvironmentally determined phenotypic features.
Unraveling an old cloak (2010)

There is a rich collection of literature that aims at protecting the privacy of users querying location-based services. One of the most popular location privacy techniques consists in cloaking users' locations such that k users appear as potential senders of a query, thus achieving k-anonymity. This paper analyzes the effectiveness of k-anonymity approaches for protecting location privacy in the presence of various types of adversaries. The unraveling of the scheme unfolds the inconsistency between its components, mainly the cloaking mechanism and the k-anonymity metric. We show that constructing cloaking regions based on the users' locations does not reliably relate to location privacy, and argue that this technique may even be detrimental to users' location privacy. The uncovered flaws imply that existing k-anonymity scheme is a tattered cloak for protecting location privacy.
Characterization of nitrogen metabolite signalling in <i>Aspergillus</i> via the regulated degradation of <i>areA</i> mRNA (2001)

AreA is the principal transcription factor involved in determining nitrogen utilization in Aspergillus nidulans. NH4+ and Gln are utilized preferentially but in their absence, AreA acts to facilitate the expression of genes involved in metabolizing alternative nitrogen sources. It is crucial to the function of AreA that its expression is tightly modulated by the quality and availability of nitrogen sources. One signalling mechanism involves regulated degradation of the areA transcript in response to NH4+ and Gln, which provides the first direct means of monitoring nitrogen signalling in this fungus. Here we assess the specificity of the transcript degradation response, determining that it responds qualitatively to a variety of additional nitrogen sources including Asn. Furthermore, the response to Gln and NH4+ requires the same discrete region of the areA 3'-UTR but both NH4+ and Asn need to be metabolized to Gln before they are effective as a signal. However, NH4+ signalling is independent of AreA activity, unlike Gln and Asn signalling. A mutation in the structural gene for NADP-linked glutamate dehydrogenase, gdhA, which disrupts metabolism of NH4+ to Glu, is additive with mutations in two distinct regions of areA that disrupt the previously identified signalling mechanisms. The triple mutant is both strongly derepressed and expresses very high levels of nitrate reductase activity. These data suggest nitrogen metabolism in A. nidulans is in part regulated in response to the intracellular levels of Gln via the regulated degradation of areA mRNA, but the intracellular Gln level is not the sole determinant of nitrogen metabolite repression.
Effect of continuous speech and non-speech signals on stuttering frequency in adults who stutter (2011)

The inhibitory effects of continuously presented audio signals (/a/, /s/, 1,000 Hz pure-tone) on stuttering were examined. Eleven adults who stutter participated. Participants read four 300-syllable passages (i.e. in the presence and absence of the audio signals). All of the audio signals induced a significant reduction in stuttering frequency relative to the control condition (P = 0.005). A significantly greater reduction in stuttering occurred in the /a/ condition (P < 0.05), while there was no significant difference between the /s/ and 1,000 Hz pure-tone conditions (P > 0.05). These findings are consistent with the notion that the percept of a second signal as speech or non-speech can respectively augment or attenuate the potency for reducing stuttering frequency.
Initial testing (stage 1) of the proteasome inhibitor bortezomib by the pediatric preclinical testing program (2007)

Administered at its MTD in mice, bortezomib demonstrated activity against selected lines of the PPTP's ALL in vivo panel. Further studies are indicated to determine the activity of bortezomib when combined with standard agents to treat childhood ALL.
Generalizations of rough set concepts (2010)

In this paper, we generalized the notions of rough set concepts using two topological structures generated by general binary relation defined on the universe of discourse. New types of topological rough sets are initiated and studied using new types of topological sets. Some properties of topological rough approximations are studied by many propositions.
Rotation Barriers of 1‐Adamantyl‐Csp <sup>3</sup> Bonds Measured with Dynamic NMR (2019)

Abstract Adamantane is a lipophilic symmetrical hydrocarbon cage, which has been successfully used as a valuable fragment in drug design. The bond rotations of the 1‐adamantyl group in Ad‐CEt 2 OH ( 11 ) and Ad‐CEt 2 Cl ( 12 ) were investigated, and the bond rotation barriers were compared with energy values from similar compounds measured previously. Exchange rate constants were estimated from lineshape fitting of low temperature 13 C NMR data for compound 11 , and used to estimate the free energy of the 1‐adamantyl group‘s single bond rotation at 8.8 kcal mol −1 . Spectra analyses for compound 12 were hampered by impurities, and only an estimate of the respective rate constant was possible from a single coalescence point. The free energy for bond rotation of 1‐adamantyl group in 12 was found to be ∼ 10 kcal mol −1 , based on decoalescence of signals at −100 ο C. The barrier for 12 is comparable with the single bond rotation barrier of Ad‐CMe 2 Cl ( 2 ) (9.3 kcal mol −1 ), or the analogous barrier of tBu‐CMe 2 Cl ( 1 ) (10.4 kcal mol −1 ). As previously suggested, single bond rotational barriers Csp 3 ‐Csp 3 were found lower than expected when one Csp 3 is included in a bulky but rigid tertiary residue (e. g. 1‐adamantyl), compared to a smaller but more flexible residue (e. g. tert ‐butyl).