gebhart/patrepeval huggingface dataset:
Promoter DNA contacts made by the vaccinia virus early transcription factor (1991)

Vaccinia virus RNA polymerase requires the heterodimeric protein, vaccinia early transcription factor (VETF), for transcription of early gene templates in vitro. We have analyzed the vaccinia growth factor promoter sequences interacting with VETF at the nucleotide level and provide evidence that the factor contacts the DNA at two separate sites. DNase I protection analysis showed that VETF was found to nucleotides -12 to -29 relative to the transcription initiation site, and also to nucleotides +8 to +10 downstream of the initiation site. The importance of both binding sites for stable complex formation was supported by methylation interference analysis. Using synthetic oligonucleotides encoding different parts of the vaccinia growth factor promoter, it was shown that nucleotides down-stream of the transcription initiation site are required for stable complex formation. Competition binding experiments demonstrated that only the upstream binding site contributes significantly to binding specificity. Binding to two separated DNA sequences results in a bend in the promoter DNA as demonstrated by electrophoretic mobility shift analysis of permuted DNA fragments. These findings suggest that VETF activates transcription by sequence specific binding and structural alteration of the promoter DNA helix.
Enhanced Transformation of Human Immunocompetent Cells by Dibutyryl Adenosine Cyclic 3′,5′-Monophosphate (1971)

Dibutyryl adenosine cyclic 3′,5′-monophosphate(10−7−10−6 M) augmented the response (transformation, DNA synthesis, and mitosis) of normal human lymphocytes to antigenic or mitogenic stimulation. In contrast, the response of lymphocytes from patients with lymphocytic leukemia was not enhanced. Higher concentrations (10−6−10−5 M) were inhibitory with both normal and leukemic lymphocytes.
New Approches to the Intermediate Band Solar Cell Concept (2009)

The development of the intermediate band solar cell is attracting more attention every day as new results appear. In this paper we aim to describe the evolution of the research from an initial theoretical proposal to the first stages of implementation using quantum dots and the experimental proof of the concepts, followed by the effort of finding bulk intermediate band materials and cells, and the demonstration of how the achievement of an intermediate band suppresses the non-radiative recombination attributed to deep centres. Finally, the project IBPOWER that continues EC support for the intermediate band concept is also described.
Host Response to Pathogenic Bacteria at Mucosal Sites (2003)

Epithelial cells at mucosal surfaces constitute the first line of defense against pathogens. Intestinal epithelial cells (IECs) form a single layer of cells that isolate the host’ s internal milieu from gut luminal environment. IECs from the distal ileum and colon are in constant contact with a rich micro-bial flora, and yet, under normal conditions, no pathological inflammation is present. However, IECs possess the ability to discriminate between pathogenic and nonpathogenic bacteria because infection of these cells by pathogenic bacteria induces an inflammatory response. Because of the repertoire of proinflammatory molecules they produce when activated by microbial agents or proinflammatory cytokines, IECs can function as sensors of mucosal injury and actively participate in the innate immune mucosal response. Therefore, these cells play an essential role in the maintenance of a deli-cate balance between the defensive immune response and tolerance to local microflora. This chapter outlines some of the mechanisms by which IEC acts as sensor for microbial pathogens and how the proinflammatory response mediated by IEC plays a role in the pathogenesis of inflammatory bowel disease (IBD).
A real-time surface EMG decomposition system for non-invasive human-machine interfaces (2018)

Real-time surface EMG decomposition, to extract neural activity of spinal motor neurons, provides a non-invasive solution for establishing direct interfaces with the central nervous system. In this paper, we present a real-time EMG decomposition system, validate it through both synthetic and experimental high-density surface EMG (HD-sEMG) data, and demonstrate the system in an upper-limb prosthetic control scenario. The proposed system achieves (in real-time) median decomposition accuracy comparable to offline methods (within 0.5 %) with minimal utilisation of computational resources (x20 faster compared to the literature).
Soliton Logic Elements For All-Optical Processing (1987)

Two designs for fibre based optical logic and switching elements are presented. The problem of switching entire ultrashort pulses is addressed and the solution of employing dispersion to generate communication within the pulse is presented. This work shows how solitons can be exploited in the devices to give excellent switching characteristics as well as ensuring that there are no detrimental dispersive effects.
Propensity-Score Analysis Reveals a Survival Benefit with Repair of Congenital Diaphragmatic Hernia after Extracorporeal Membrane Oxygenation in Select Patients (2018)

Delaplain, Patrick MD; Zhang, Lishi MS; Harting, Matthew T. MD, MS, FAAP, FACS; Jancelewicz, Tim MD, MA, MS, FACS; Yu, Peter T. MD; Stein, James E. MD, MSc, FACS; Ford, Henri R. MD, MHA, FACS, FAAP, FRCSEng(Hon); Nguyen, Danh V. PhD; Guner, Yigit S. MD, FACS Author Information
Characterization of two forms of protein kinase C from the leukaemia cell line K562 (1989)

Conference Article| August 01 1989 Characterization of two forms of protein kinase C from the leukaemia cell line K562 R. HOFFMAN; R. HOFFMAN 1Department of Medical Oncology, Charing Cross Hospital, Fulham Palace Road, London W6 8RF, U.K. Search for other works by this author on: This Site PubMed Google Scholar A. FREEMAN; A. FREEMAN 1Department of Medical Oncology, Charing Cross Hospital, Fulham Palace Road, London W6 8RF, U.K. Search for other works by this author on: This Site PubMed Google Scholar E. S. NEWLANDS E. S. NEWLANDS 1Department of Medical Oncology, Charing Cross Hospital, Fulham Palace Road, London W6 8RF, U.K. Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1989) 17 (4): 720. https://doi.org/10.1042/bst0170720 Article history Received: November 23 1988 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn MailTo Cite Icon Cite Get Permissions Citation R. HOFFMAN, A. FREEMAN, E. S. NEWLANDS; Characterization of two forms of protein kinase C from the leukaemia cell line K562. Biochem Soc Trans 1 August 1989; 17 (4): 720. doi: https://doi.org/10.1042/bst0170720 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search Keywords: PKC, protein kinase C, PMA, phorbal 12-myristate 13-acetate, PS, phosphatidylserine This content is only available as a PDF. © 1989 Biochemical Society1989 Article PDF first page preview Close Modal You do not currently have access to this content.
Les amibes libres, un réservoir environnemental de <em>Mycobacterium avium</em> ssp. <em>paratuberculosis</em> ? (2016)

Session 1 - Mécanismes de virulence et d'adaptation des pathogènes à l'hôte
CD148 Tyrosine Phosphatase Promotes Cadherin Cell Adhesion (2014)

CD148 is a transmembrane tyrosine phosphatase that is expressed at cell junctions. Recent studies have shown that CD148 associates with the cadherin/catenin complex and p120 catenin (p120) may serve as a substrate. However, the role of CD148 in cadherin cell-cell adhesion remains unknown. Therefore, here we addressed this issue using a series of stable cells and cell-based assays. Wild-type (WT) and catalytically inactive (CS) CD148 were introduced to A431D (lacking classical cadherins), A431D/E-cadherin WT (expressing wild-type E-cadherin), and A431D/E-cadherin 764AAA (expressing p120-uncoupled E-cadherin mutant) cells. The effects of CD148 in cadherin adhesion were assessed by Ca2+ switch and cell aggregation assays. Phosphorylation of E-cadherin/catenin complex and Rho family GTPase activities were also examined. Although CD148 introduction did not alter the expression levels and complex formation of E-cadherin, p120, and β-catenin, CD148 WT, but not CS, promoted cadherin contacts and strengthened cell-cell adhesion in A431D/E-cadherin WT cells. This effect was accompanied by an increase in Rac1, but not RhoA and Cdc42, activity and largely diminished by Rac1 inhibition. Further, we demonstrate that CD148 reduces the tyrosine phosphorylation of p120 and β-catenin; causes the dephosphorylation of Y529 suppressive tyrosine residue in Src, a well-known CD148 site, increasing Src activity and enhancing the phosphorylation of Y228 (a Src kinase site) in p120, in E-cadherin contacts. Consistent with these findings, CD148 dephosphorylated both p120 and β-catenin in vitro. The shRNA-mediated CD148 knockdown in A431 cells showed opposite effects. CD148 showed no effects in A431D and A431D/E-cadherin 764AAA cells. In aggregate, these findings provide the first evidence that CD148 promotes E-cadherin adhesion by regulating Rac1 activity concomitant with modulation of p120, β-catenin, and Src tyrosine phosphorylation. This effect requires E-cadherin and p120 association.
Vitamin D3 promotes the differentiation of colon carcinoma cells by the induction of E-cadherin and the inhibition of <b>β</b>-catenin signaling (2001)

The beta-catenin signaling pathway is deregulated in nearly all colon cancers. Nonhypercalcemic vitamin D3 (1alpha,25-dehydroxyvitamin D(3)) analogues are candidate drugs to treat this neoplasia. We show that these compounds promote the differentiation of human colon carcinoma SW480 cells expressing vitamin D receptors (VDRs) (SW480-ADH) but not that of a malignant subline (SW480-R) or metastasic derivative (SW620) cells lacking VDR. 1alpha,25(OH)2D(3) induced the expression of E-cadherin and other adhesion proteins (occludin, Zonula occludens [ZO]-1, ZO-2, vinculin) and promoted the translocation of beta-catenin, plakoglobin, and ZO-1 from the nucleus to the plasma membrane. Ligand-activated VDR competed with T cell transcription factor (TCF)-4 for beta-catenin binding. Accordingly, 1alpha,25(OH)2D(3) repressed beta-catenin-TCF-4 transcriptional activity. Moreover, VDR activity was enhanced by ectopic beta-catenin and reduced by TCF-4. Also, 1alpha,25(OH)2D(3) inhibited expression of beta-catenin-TCF-4-responsive genes, c-myc, peroxisome proliferator-activated receptor delta, Tcf-1, and CD44, whereas it induced expression of ZO-1. Our results show that 1alpha,25(OH)2D(3) induces E-cadherin and modulates beta-catenin-TCF-4 target genes in a manner opposite to that of beta-catenin, promoting the differentiation of colon carcinoma cells.
Early toll‐like receptor 4 blockade reduces ROS and inflammation triggered by microglial pro‐inflammatory phenotype in rodent and human brain ischaemia models (2019)

TLR4 signalling has a causal role in the inflammation associated with a poor post-stroke outcome. Importantly, its inhibition by eritoran (E5564) provides neuroprotection both in vitro and in vivo, including in human tissue, suggesting a promising new therapeutic approach for ischaemic stroke.
Achievable performance of sampled-data controllers with input and output delays (2002)

Sampled-data systems often entail transport delays due to communication lags. In this paper we synthesize sampled-data controllers that account a priori for such delays. Our approach which accounts for measurement noise and intersample behavior, allows us to investigate the effect of delays on the achievable performance of sampled-data systems. Several examples are given to illustrate the effect of large delays on system performance. Depending upon measurement disturbance levels, numerical examples show that large delays may be incurred without a significant loss in performance.
Bulk and microscopic properties of copolymer networks in mixed aqueous solvents (1992)

Abstract Amphiphilic films and hydrogels have been prepared from ethanol/water solutions containing a hydrocarbon‐grafted water‐soluble cellulose ether. These materials are characterized by dispersed hydrophobic microdomains which form spontaneously in the solvent due to the inherent incompatibility of the side chains with water. At low applied shear stress, the microdomains behave as temporary linkages of finite lifetimes, imparting viscoelastic properties to the networks. The molecular weight between microdomains was found to be independent of the volume fraction of polymer in the gel, and the number of linkages per backbone ranges from 22.8 ± 1.3 to 26.2 ± 1.5 over the frequency range 30–50 rad/s. The behavior of the solutions and gels was characterized using fluorescence and dynamic rheological measurements. It was demonstrated that the microdomains are capable of sequestering water‐insoluble solutes. © 1992 John Wiley & Sons, Inc.
The Hypocholesterolizing Effects of Drugs (1956)

The hypocholesterolizing effect on serum and tissue cholesterol of α-phenylbutyramide (PB) is evident in the cholesterol-fed fowl. PB and Gallogen (the diethanolamine salt of the mono-(+)-camphoric acid ester of α,4-dimethylbenzyl alcohol) are mutually antagonistic with respect to this effect, while PB and soybean sterols are additive. The latter combination is approximately as effective as a Gallogen-soybean sterol combination, but has distinct toxic effects that are not observed with the Gallogen-soybean sterol combination.
Genetic variation of NEDD4L is associated with essential hypertension in female Kazakh general population: a case-control study (2009)

The genetic variations of NEDD4L may be associated with essential hypertension in females in the Kazakh general population.
ChemInform Abstract: Non‐siliceous Mesostructured and Mesoporous Materials (2001)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Effect of Phospholipids and Unsaponifiable Matter on Oxidative Stability of Milk Fat (1958)

Stability of milk fat samples prepared by different methods was measured by determining the length of the induction period at 80 ---+ 0.5 C. Fat extracted from cream or buttermilk with a 2:3 ether-pentane mixture had greater stability than churned fat. This was attributed to synergism between phospholipids and other antioxidants or synergists extracted from membrane material and plasma, and was confirmed by the stabilizing effects of adding to churned fat different levels of phospholipids and/or nnsaponifiable matter. Churned fat contained no phospholipids. Unsaponifiable matter (free of acids) alone, added to churned and extracted fats, increased fat stability. Among the known constituents of the unsaponifiable matter, beta-carotene acted as a prooxidant, and cholesterol had no significant effect. Alpha-tocopherol was the only unsaponifiable constituent tested that acted synergistically with phospholipids in stabilizing churned fat. However, differences in the amounts of tocopherol could not entirely account for the differences in stability among different extracted fats. Oxidative deterioration was detected organoleptieally in churned fat before the end of the induction period and at peroxide values less than 1.00.
Crystal structures of (<i>n</i>-Pr<sub>4</sub>N)<sub>2</sub>SnCl<sub>6</sub>, (<i>n</i>-Pr<sub>4</sub>N)[TeCl<sub>4</sub>(OH)], (<i>n</i>-Pr<sub>4</sub>N)<sub>2</sub>[Te<sub>2</sub>Cl<sub>10</sub>] (nominal), and (<i>n</i>-Pr<sub>4</sub>N)<sub>2</sub>[Se<sub>2</sub>O<sub>2</sub>Cl<sub>6</sub>], with observations on Z<sub>2</sub>L<sub>10</sub><sup>2<i>n</i>−</sup> and Z<sub>2</sub>L<sub>8</sub><sup>2−</sup> dimers in general (1992)

The crystal structures of the following four compounds have been determined: (n-Pr 4 N) 2 SnCl 6 (A; Pbca, a = 29.073 Å, b = 15.825 Å, c = 14.851 Å, Z = 8), (n-Pr 4 N)[TeCl 4 (OH)] (B; C2/c or Cc, a = 10.800 Å, b = 17.429 Å, c = 10.604 Å, β = 93.81°, Z = 4), (n-Pr 4 N) 2 [Te 2 Cl 10 ] (C, nominal composition; [Formula: see text], a = 9.635 Å, b = 10.900 Å, c = 11.547 Å, α = 73.34°, β = 70.44°, γ = 68.15°, Z = 1), and (n-Pr 4 N) 2 [Se 2 O 2 Cl 6 (D; [Formula: see text], a = 9.252 Å, b = 11.691 Å, c = 18.304 Å, α = 110.18°, β = 92.57°, γ = 97.72°, Z = 2). B contains a square-pyramidal TeCl 4 OH) − anion. The actual composition of the centrosymmetric μ 2 -dimer in C is Te 2 Cl 8 (Cl, OH) 2 2− , the replacement of Cl by OH being at the axial ligand positions of the TeCl 5 − halves of the dimer. In the centrosymmetric μ 2 -Se 2 O 2 Cl 6 2− dimer in D the Se—O bonds are perpendicular to the Se(Cl) 2 Se bridge plane and trans to the presumed lone-electron pairs on the Se atoms. It is shown that the Z—Hal bonds in the known centrosymmetric homoligand μ 2 -Z 2 Hal 10 2n− (Z = Se, Te) and μ 2 -Z 2 Hal 8 2− (Z = As, Sb, Bi) as well as in the μ 2 -Se 2 O 2 Hal 6 2− dimers exhibit a uniform stereochemistry, in which the d(Z—Hal) bond lengths in pairs of opposing bonds trans to each other are consistently related by the trans effect (TE). Empirical quantification of Te for the Se and Te dimers is exploited to authenticate the composition of the dimer in situations where the nature of the ligand is in doubt (in the present case on partial hydrolysis). The asymmetry of the Z(Hal) 2 Z double bridge is examined in relation to the overall stereochemistry of the dimer. Also examined are circumstances that may lead to the occurrence, in a crystal, of a Z 2 Hal 10 2n− dimer on the one hand and of a discrete ZHal 5 n− monomer on the other, in situations which otherwise are chemically closely similar. TLS analysis of the thermal motion is used to judge the strength of association of the two monomer halves in a μ 2 -dimer, with results that are important when correcting bond lengths for the effects of thermal motion. A variety of general observations on μ 2 -dimers in crystals is proffered for good measure.
Optimal Complete Vehicle Control for Fuel Efficiency (2016)

CONVENIENT is a project where prediction and integrated control are applied on several subsystems with electrified actuators. The technologies developed in this project are applied to a long-haul tractor and semi-trailer combination. A Volvo truck meeting the Eu6 emission standard is rebuilt with a number of controllable electrified actuators. An e-Horizon system collects information about future road topography and speed limits. Controllable aerodynamic wind deflectors reduce the wind drag. The tractor is also equipped with a full digital cluster for human machine interface development. A primary project goal is to develop a model-based optimal controller that uses predictive information from the e-Horizon system in order to minimize fuel consumption. Several energy buffers are controlled in an integrated and optimal way using model predictive control. Several buffers are considered, such as the cooling system, the battery, and the vehicle kinetic energy. This paper presents details on the model predictive controller of the battery system and of the cooling system. Another project goal is to reduce fuel consumption by using adaptive aerodynamics. Controllers are developed that automatically sets an optimal roof deflector angle and the optimal side deflector angle. The results presented in this paper are encouraging. A third focus is the human machine interface and especially the communication between the driver and the control system during driving. This project develops a driver interface that encourages the driver to use the adaptive cruise controller when appropriate. The CONVENIENT project will be finalized this year. This paper presents the main project findings.
Zur Existenz des Tellur(II)‐oxyds (1948)

Abstract Durch Reaktion von Tellur mit Tellurdioxyd bei verschiedenen Temperaturen, durch thermische Zersetzung von TeSO 3 , sowie durch Oxydation von feinverteiltem Tellur mit Stickstoffdioxyd, gelingt es nicht, TeO darzustellen. Seine Existenz wird angezweifelt.
Evaluating the potential of the wheat aleurone fraction in humans using <sup>1</sup>H NMR-based metabolomic analysis (2010)

An abstract is not available for this content. As you have access to this content, full HTML content is provided on this page. A PDF of this content is also available in through the 'Save PDF' action button.
Tissue-Volume Preserving Deformable Image Registration for 4DCT Pulmonary Images (2016)

We propose a 4D (three spatial dimensions plus time) tissue-volume preserving non-rigid image registration algorithm for pulmonary 4D computed tomography (4DCT) data sets to provide relevant information for radiation therapy and estimate pulmonary ventilation. The sum of squared tissue volume difference (SSTVD) similarity cost takes into account the CT intensity changes of spatially corresponding voxels, which is caused by the variations of fraction of tissue within voxels throughout the respiratory cycle. The proposed 4D SSTVD registration scheme considers the entire dynamic 4D data set simultaneously, using both spatial and temporal information. We employed a uniform 4D cubic B-spline parametrization of the transform and a temporally extended linear elasticity regularization of deformation field to ensure temporal smoothness and thus biological plausibility of estimated deformation. We used a multiresolution multi-grid registration framework with limitedmemory Broyden Fletcher Goldfarb Shanno (L-BFGS) optimization procedure for rapid convergence and limited memory consumption. We conducted experiments using synthetic 2D+t images and clinical 4DCT pulmonary data sets and evaluated accuracy and temporal smoothness of the proposed method via manually annotated landmarks.
Multiple Vertebral Osteotomies in the Treatment of Rigid Adult Spine Deformities (2001)

This study demonstrates multiple vertebral osteotomies (anterior and/or posterior) in the management of rigid adult spine deformities and deformity correction with an acceptable complication rate. Use of vertebral osteotomies for patients undergoing revision spine surgery is a safe and reasonable approach to obtain an arthrodesis.
Relationship Between Generation of CO and CO2 and Toxicity of the Environments Created by Materials in Flaming and Nonflaming Fires and Effect of Fire Ventilation (1990)

In fires, combustion behavior of materials, generation rates, and concentrations of fire products depend on many factors, such as the chemical structures of materials (aliphatic to aromatic to highly halogenated), fire ventilation, airflow and mixing, shape, size and arrangement of the materials, heat flux received by the materials, etc. Thus, toxicity of the environment is expected to depend on these factors. This paper examines the relationship between the combustion behavior of materials and the toxicity of the environment. In this analysis, only CO and CO2 are used, using the toxicity relationship suggested by Levin et al. [3] on the synergism between CO and CO2 and data measured in the Factory Mutual Research Corp. (FMRC) small-scale flammability apparatus.
High–Energy Astrophysics with Lobster Eye X-ray ASM (2005)

We report on astrophysical aspects of fully innovative very wide–field X-ray telescopes with high sensitivity. They are expected to contribute essentially to study of various astrophysical objects such as AGN, SNe, Gamma-ray bursts (GRBs), X-ray flashes (XRFs), galactic binary sources, stars, CVs, X-ray novae, various transient sources, etc.