gebhart/patrepeval huggingface dataset:
The time course of epidermal nerve fibre regeneration: studies in normal controls and in people with diabetes, with and without neuropathy (2004)

We sought to develop and validate a standardized cutaneous nerve regeneration model and to define the rate of epidermal nerve fibre (ENF) regeneration first in healthy control subjects and then in neuropathic and neuropathy-free subjects with diabetes. Next, we assessed the effect of different factors on the rate of nerve fibre regeneration and investigated whether such an approach might offer insight into novel trial designs and outcome measures. All subjects had a standardized topical capsaicin dressing applied to the distal lateral thigh. ENF densities derived from skin biopsies were determined at baseline, after capsaicin treatment and at reinnervation time points. For each subject, the best fit line from post-denervation data was determined and the slope was used as the rate of regeneration. In healthy control subjects, regeneration was correlated with psychophysical sensory testing, electron microscopy studies and immunohistochemistry with alternative axonal membrane markers. Topical capsaicin application produced complete or nearly complete denervation of the epidermis in both control subjects and people with diabetes. The rate of regeneration was associated with the baseline ENF density (P < 0.001), but not age (P = 0.75), gender (P = 0.18), epidermal thickness (P = 0.4) or post-capsaicin treatment density (P = 0.7). ENF regeneration, as determined by recovery of ENF density, occurred at a rate of 0.177 +/- 0.075 fibres/mm/day in healthy control subjects and was significantly reduced in subjects with diabetes (0.074 +/- 0.064, P < 0.001) after adjusting for changes in baseline ENF density. Among subjects with diabetes, the presence of neuropathy was associated with a further reduction in regenerative rate (0.10 +/- 0.07 versus 0.04 +/- 0.03, P = 0.03), though diabetes type (P = 0.7), duration of diabetes (P = 0.3) or baseline glycated haemoglobin (P = 0.6) were not significant. These results have several implications. First, topical capsaicin application can produce a uniform epidermal nerve fibre injury that is safe and well tolerated, and offers an efficient strategy to measure and study nerve regeneration in man. Secondly, using our techniques, reduced rates of nerve regeneration were found in people with diabetes without evidence of neuropathy and indicate that abnormalities in peripheral nerve function are present early in diabetes, before signs or symptoms develop. These results suggest that regenerative neuropathy trials could include non-neuropathic subjects and that trial duration can be dramatically shortened.
Composite vs Ceramic Computer-aided Design/Computer-assisted Manufacturing Crowns in Endodontically Treated Teeth: Analysis of Marginal Adaptation (2013)

CAD-CAM crowns fabricated from millable composite resin blocks (Paradigm MZ100) offer a superior option to all-ceramic crowns (IPS Empress CAD).
A 3D Frictional Segment-to-Segment Contact Method for Large Deformations and Quadratic Elements (2004)

Node-on-segment contact is the most common form of contact used today but has many deficiencies ranging from potential locking to non-smooth behavior with large sliding. Furthermore, node-on-segment approaches are not at all applicable to higher order discretizations (e.g. quadratic elements). In a previous work, [3, 4] we developed a segment-to-segment contact approach for eight node hexahedral elements based on the mortar method that was applicable to large deformation mechanics. The approach proved extremely robust since it eliminated the over-constraint that caused 'locking' and provided smooth force variations in large sliding. Here, we extend this previous approach to treat frictional contact problems. In addition, the method is extended to 3D quadratic tetrahedrals and hexahedrals. The proposed approach is then applied to several challenging frictional contact problems that demonstrate its effectiveness.
Impact of Intravenous Loop Diuretics on Outcomes of Patients Hospitalized with Acute Decompensated Heart Failure: Insights from the ADHERE Registry (2008)

The optimal use of diuretics in decompensated heart failure remains uncertain. We analyzed data from the ADHERE registry to look at the impact of diuretic dosing. 62,866 patients receiving <160 mg and 19,674 patients > or =160 mg of furosemide were analyzed. The patients receiving the lower doses had a lower risk for in-hospital mortality, ICU stay, prolonged hospitalization, or adverse renal effects. These findings suggest that future studies should evaluate strategies for minimizing exposure to high doses of diuretics.
The acquisition of humoral immune responses targeting Plasmodium falciparum sexual stages in controlled human malaria infections (2022)

Individuals infected with <i>P. falciparum</i> develop antibody responses to intra-erythrocytic gametocyte proteins and exported gametocyte proteins present on the surface of infected erythrocytes. However, there is currently limited knowledge on the immunogenicity of gametocyte antigens and the specificity of gametocyte-induced antibody responses. In this study, we assessed antibody responses in participants of two controlled human malaria infection (CHMI) studies by ELISA, multiplexed bead-based antibody assays and protein microarray. By comparing antibody responses in participants with and without gametocyte exposure, we aimed to disentangle the antibody response induced by asexual and sexual stage parasites. We showed that after a single malaria infection, a significant anti-sexual stage humoral response is induced in malaria-naïve individuals, even after exposure to relatively low gametocyte densities (up to ~1,600 gametocytes/mL). In contrast to antibody responses to well-characterised asexual blood stage antigens that were detectable by day 21 after infection, responses to sexual stage antigens (including transmission blocking vaccine candidates Pfs48/45 and Pfs230) were only apparent at 51 days after infection. We found antigens previously associated with early gametocyte or anti-gamete immunity were highly represented among responses linked with gametocyte exposure. Our data provide detailed insights on the induction and kinetics of antibody responses to gametocytes and identify novel antigens that elicit antibody responses exclusively in individuals with gametocyte exposure. Our findings provide target identification for serological assays for surveillance of the malaria infectious reservoir, and support vaccine development by describing the antibody response to leading vaccine antigens after primary infection.
CARS Temperature Measurements in a Combustion-Heated Supersonic Jet (2009)

Measurements were made in a combustion-heated supersonic axi-symmetric free jet from a nozzle with a diameter of 6.35 cm using dual-pump Coherent Anti-Stokes Raman Spectroscopy (CARS). The resulting mean and standard deviation temperature maps are presented. The temperature results show that the gas temperature on the centerline remains constant for approximately 5 nozzle diameters. As the heated gas mixes with the ambient air further downstream the mean temperature decreases. The standard deviation map shows evidence of the increase of turbulence in the shear layer as the jet proceeds downstream and mixes with the ambient air. The challenges of collecting data in a harsh environment are discussed along with influences to the data. The yield of the data collected is presented and possible improvements to the yield is presented are discussed.
Anion Exchange Studies. XXXI. Adsorption of Zn(II) and Ga(III) from HCl Solutions in the Temperature Range 25 to 150°<sup>1</sup> (1960)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAnion Exchange Studies. XXXI. Adsorption of Zn(II) and Ga(III) from HCl Solutions in the Temperature Range 25 to 150°1Kurt A. Kraus and Richard J. RaridonCite this: J. Am. Chem. Soc. 1960, 82, 13, 3271–3276Publication Date (Print):July 1, 1960Publication History Published online1 May 2002Published inissue 1 July 1960https://doi.org/10.1021/ja01498a010RIGHTS & PERMISSIONSArticle Views83Altmetric-Citations21LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (636 KB) Get e-Alerts Get e-Alerts
Adaptación de la vinificación en tinto en función del potencial polifenólico de las uvas: experiencias realizadas en la vendimia 2001 (2003)

En la vendimia 2001 se elaboraron en escala reducida vinos tintos de Tannat, Cabernet-Sauvignon y Merlot, utilizando uvas provenientes de tres vinedos de cada variedad. Se estimaron los contenidos de antocianos extraibles, potencial total en antocianos y riqueza fenolica de las uvas, de acuerdo con Glories y Augustin (1993). Posteriormente, se hicieron extracciones de los hollejos, con una solucion de etanol al 12%, pH 3.2, y se determinaron periodicamente las concentraciones de antocianos y polifenoles totales. Se efectuaron dos vinificaciones por vinedo, con 50 kg de uva en cada una. Los contenidos fenolicos totales y antocianicos de los mostos se determinaron cada 24 horas, desde la molienda hasta el momento del descube. La duracion de las maceraciones fue decidida en funcion de los indices determinados en las uvas y de las extracciones de polifenoles en el laboratorio y en la bodega. Los vinos fueron analizados a los dos meses de finalizada la fermentacion, determinando su color y composicion fenolica. Las uvas y vinos Tannat tuvieron contenidos significativamente mayores de polifenoles. Se encontro una alta correspondencia entre los contenidos fenolicos de uva enteras, hollejos, mostos y vinos. La correspondencia de las caracteristicas de los vinos con los indices determinados en uvas, hollejos y mostos confirma que la estimacion del potencial polifenolico de las uvas es un dato esencial para dirigir mas adecuadamente la vinificacion en tinto.
Studies on the biosynthesis of bialaphos (SF-1293) 1. Incorporation of 13C- and 2H-labeled precursors into bialaphos. (1982)

2-Phosphinomethylmalic acid (PMM) synthase catalyzes the condensation of phosphinopyruvic acid (PPA), an analog of oxalacetic acid, and acetyl-CoA to form PMM. The enzyme was purified approximately 700-fold from a cell-free extract of Streptomyces hygroscopicus SF1293, a bialaphos producing organism, to an electrophoretically homogeneous state. The purified PMM synthase has a subunit molecular weight of 48, 000 by SDS-polyacrylamide gel electrophoresis and a native molecular weight of 90, 000 - 98, 000 by gel filtration. PMM synthase was relatively unstable, showed maximum activity at pH 8.0 and 30°C, and was inhibited strongly by p-chloromercuribenzoate, iodoacetamide and EDTA. Enzyme activity suppressed by EDTA was completely restored by adding Co+ + or Mn+ + and partially restored by addition of Ca+ +, Fe+ + or Mg+ +. The specific substrates of this enzyme are PPA or oxalacetic acid in addition to acetyl-CoA. The enzyme does not catalyze the liberation of Co A from acetyl-CoA in the presence of α-keto acids, such as pyruvate, α-ketoglutarate, deamino-α-ketodemethylphosphinothricin or phosphonopyruvate. The condensation reaction did not take place when propionyl-CoA or butyryl-CoA was used as a substrate in place of acetyl-CoA. The Km values of the enzyme were 0.05 mM for acetyl-CoA, 0.39 mM for PPA and 0.13 mM for oxalacetate. PMM synthase is very similar to (R)-citrate synthase of Clostridium in the inhibition pattern by sulfhydryl compounds, its metal ion requirement and stereospecificity; unlike CR)-citrate synthase PMM synthase was not inhibited by oxygen.
Preoperative Radiotherapy Combined With Intraoperative Radiotherapy Improve Results of Total Mesorectal Excision in Patients With T3 Rectal Cancer (2004)

Preoperative radiotherapy plus intraoperative radiotherapy associated with total mesorectal excision reduce local recurrence rate and improve survival in T3 rectal cancer compared with total mesorectal excision alone.
Nuclear translocation of CAR is dependent on ubiquitination and proteasome‐mediated degradation of CAR cytoplasmic retention protein (CCRP) (2006)

The constitutive active/androstane receptor (CAR) plays an important role as a coordinate transcription factor in the regulation of various hepatic metabolic pathways for chemicals such as drugs, glucose, fatty acids, bilirubin, and bile acids. Currently, it is known that in its inactive state, CAR is retained in the cytoplasm in a protein complex with Hsp90 and the tetratricopeptide repeat protein CAR cytosolic retention protein (CCRP). Upon activation by phenobarbital (PB) or the PB‐like inducer 1,4‐bis[2‐(3,5‐dichloropyridyloxy)]‐benzene (TCPOBOP), CAR translocates into the nucleus. We have identified ubiquitin‐dependent degradation of CCRP as an important signal for the nuclear translocation of CAR. Treatment with the proteasome inhibitor MG132 (5 μM) causes CAR to accumulate in the cytosol of transfected HepG2 cells. In the presence of MG132, TCPOBOP increases CCRP ubiquitination in HepG2 cells co‐expressing CAR, while CAR itself is not ubiquitinated. Moreover, immunoprecipitation experiments show that in the presence of MG132, CAR remains bound to CCRP. Consistent with continued retention of CAR by CCRP upon activator treatment in the presence of MG132, we observe the attenuation of CAR‐mediated transcriptional activation on both reporter constructs and the endogenous CYP2B6 gene in HepG2 cells. Collectively, these data suggest that ubiquitin‐proteasomal regulation of CCRP is an important determinant for the ability of CAR to respond to PB or PB‐like inducers.
Land treatment of petroleum-refinery sludges. Final report, April 1980-June 1983 (1984)

Petroleum API Separator sludge was applied to field plots to evaluate optimization of loading rates and frequencies for waste disposal by land treatment. Loading rates 3 to 13 wt. % and frequencies 1 to 12, respectively, per year were studied over an 18 month period. Total oil losses were proportional to the amount applied and averaged 54% over the study period. Saturates fraction loss was highest followed by aromatics, polars, and asphaltenes. Volatile losses were substantial at application, but relatively small over the long-term. Biodegradation of the oil followed first order kinetics with a rate coefficient of 0.003/day. Heavy metals were immobile in the top 30 cm zone of incorporation. Facility design factors are discussed relating to field equipment operation, oil percolation prevention, runoff control, and proper tillings.
ChemInform Abstract: The Use of Hydrazones for Efficient Mannich Type Coupling with Aldehydes and Secondary Amines. (2000)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
interleukin‐1β and tumor necrosis factor‐α stimulate synergistically the expression of monocyte chemoattractant protein‐1 in fibroblastic cells derived from human periodontal ligament (1996)

The present study demonstrates that interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF-alpha) induce and synergistically stimulate monocyte chemoattractant protein-1 (MCP-1) expression in fibroblasts from human periodontal ligament. IL-1 beta and TNF-alpha induced in a dose-dependent manner the expression of the MCP-1 gene in the fibroblasts from the human periodontal ligament. However, such an inducing effect was not observed with IL-6 and interferon-gamma. The peak expression of the MCP-1 gene by IL-1 beta or TNF-alpha was observed at 3 h after initiation of their treatment. Furthermore, IL-1 beta in combination with TNF-alpha synergistically stimulated the MCP-1 gene expression in the cells. We also observed significant chemotactic activity for human monocytes in conditioned medium of fibroblasts from the human periodontal ligament treated with both cytokines. The stimulated chemotactic activity induced by these cytokines depended on both dose and treatment time. The chemotactic activity in conditioned medium of IL-1 beta-treated fibroblasts from the human periodontal ligament was neutralized by antiserum specific for MCP-1 protein. The MCP-1 gene product in conditioned medium of IL-1 beta-treated fibroblasts from the human periodontal ligament was shown to have a molecular mass of 11,000 Da by immunoprecipitation with the specific antiserum, and IL-1 beta also stimulated synergistically MCP-1 protein expression in combination with TNF-alpha. These results suggest that IL-1 beta and TNF-alpha may contribute to the infiltration of monocytes into inflammatory sites of periodontal ligament tissues via the MCP-1 gene product produced by fibroblasts from the human periodontal ligament.
The influence of InGaAs quantum dots on GaAs P-I-N solar cell dark current properties (2011)

An analysis of the temperature dependent dark current and spectral response characteristics of an In <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">0.5</sub> Ga <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">0.5</sub> As/GaAs quantum dot solar cell has been performed to determine the dominant physical processes that lead to a reduction of the open circuit voltage compared with reference single junction GaAs solar cells.
Microarray analysis of in vitro pericyte differentiation reveals an angiogenic program of gene expression (2004)

The vasculature consists of endothelial cells (ECs) lined by pericyte/vascular smooth muscle cells (vSMCs). Pericyte/vSMCs provide support to the mature vasculature but are also essential for normal blood vessel development. To determine how pericyte-EC communication influences vascular development, we used the well-established in vitro model of TGFbeta-stimulated differentiation of 10T1/2 cells into pericyte/vSMCs. Microarray analysis was performed to identify genes that were differentially expressed by induced vs. uninduced 10T1/2 cells. We discovered that these cells show an angiogenic program of gene expression, with up-regulation of several genes previously implicated in angiogenesis, including VEGF, IL-6, VEGF-C, HB-EGF, CTGF, tenascin C, integrin alpha5, and Eph receptor A2. Up-regulation of some genes was validated by Western blots and immunocytochemistry. We also examined the functional significance of these gene expression changes. VEGF and IL-6 alone and in combination were important in 10T1/2 cell differentiation. Furthermore, we used a coculture system of 10T1/2 and human umbilical vein ECs (HUVECs), resulting in the formation of cordlike structures by the HUVECs. This cordlike structure formation was disrupted when neutralizing antibodies to VEGF or IL-6 were added to the coculture system. The results of these studies show that factors produced by pericytes may be responsible for recruiting ECs and promoting angiogenesis. Therefore, a further understanding of the genes involved in pericyte differentiation could provide a novel approach for developing anti-angiogenic therapies.
Evaluating the neuroprotective impact of senolytic drugs on human vision (2020)

ABSTRACT Glaucoma, a chronic neurodegenerative disease of retinal ganglion cells (RGCs), is a leading cause of irreversible blindness worldwide. Its management currently focuses on lowering intraocular pressure to slow disease progression. However, disease-modifying, neuroprotective treatments for glaucoma remain a major unmet need. Recently, senescent cells have been observed in glaucomatous eyes, exposing a potential pathway for alternative glaucoma therapies. Prior studies demonstrated that targeting senescent RGCs for removal (i.e., a senolytic approach) protected healthy RGCs and preserved visual function in a mouse ocular hypertension model. However, the effects of senolytic drugs on vision in human patients are unknown. Here, we used existing clinical data to conduct a retrospective cohort study in 28 human glaucoma patients who had been exposed to senolytics. Senolytic exposure was not associated with decreased visual acuity, elevated intraocular pressure, or documentation of senolytic-related adverse ocular effects by treating ophthalmologists. Additionally, patients exposed to senolytics (n=9) did not exhibit faster progression of glaucomatous visual field damage compared to matched glaucoma patients (n=26) without senolytic exposure. These results suggest that senolytic drugs do not carry significant ocular toxicity and provide further support for additional evaluation of the potential neuroprotective effects of senolytics on glaucoma and other neurodegenerative diseases.
5α-Dihydrotestosterone (DHT) Receptors in Rat Brain and Pituitary Cell Nuclei (1977)

Gonadectomized-adrenalectomized (GX-ADX) adult male rats were injected iv with 2-4 mug/kg [1,2-3H]DHT and sacrificed 2 h later. Whole tissue homogenates and purified cell nuclear fractions were prepared from various brain regions and the pituitary and analyzed for radioactivity. Cell nuclei from pituitary and most limbic-hypothalamic regions (but not cerebral cortex) concentrated radioactivity (per unit protein) over whole tissue levels. The highest levels of nuclear-associated radioactivity were present in pituitary, hypothalamic and septal tissues. Analysis of radioactivity by double isotope dilution, chromatography, and recrystallization revealed that unmetabolized DHT represented 96 and 95% of the nuclear-associated radioactivity in pituitary and pooled limbic-hypothalamic structures, respectively. Simultaneously administered 100-fold molar excesses of unlabeled 5betaDHT, progesterone or corticosterone did not reduce nuclear-retained [3H]DHT, while unlabeled 5alphaDHT competed strongly. The anti-androgen, cyproterone acetate, competed at doses capable of of blocking androgen-mediated neuroendocrine effects. Levels of nuclear-retained [3H]DHT were negligible in intact males, but rose markedly 24 h after castration-adrenalectomy, remaining stable for 2 weeks post-operatively. Density gradient centrifugation of pituitary or brain cell nuclear salt extracts (0.4M KCl) revealed the [3H]DHT bound to a macromolecule sedimenting at 3--4S. The characteristics of DHT brain and pituitary cell nuclear binding are compared with the respective characteristics of neural cytosol and ventral prostate nuclear androgen binding components. These preliminary data suggest the existence of the functional, steroid-specific, stereospecific cell nuclear androgen receptor in the adult rat brain and pituitary.