gebhart/patrepeval huggingface dataset:
<i>In vitro</i> model for the study of necrosis and apoptosis in native cartilage (2002)

Apoptosis plays a role in everything from early development to ageing and in a host of disease states. Studying this important process in the in vivo state is critical, to understand its varied role and to open further avenues of therapeutic intervention. The present paper presents an ex vivo bovine articular cartilage model to study apoptotic and necrotic processes following acute injury. Ex vivo bovine articular cartilage was assessed 1, 3 and 6 days following holmium : YAG laser treatment (780 mJ). Markers to visualize cell viability, caspase-3 activity, changes in mitochondrial membrane potential and the degree of DNA fragmentation (TUNEL assay) were used alone or in various combinations. Standard histology and transmission electron microscopy (TEM) were also performed for a more comprehensive assessment. A significant progression (p < 0.05) of ethidium/caspase-3-positive signal depth at day 3 preceded a significant increase (p < 0.05) in TUNEL signal depth by day 6. The mitochondrial matrix marker CMXRos was shown to provide an alternative to calcein-AM for assessing cell viability. The identification of chondrocyte apoptosis morphology by TEM was not conclusive. Nevertheless, TEM revealed that cells which were clearly necrotic also stained positively for TUNEL, thus indicating the risk of using TUNEL alone for the assessment of apoptosis. The model described here allows the rapid, spatial and temporal determination of cell viability and of apoptotic and necrotic processes in whole-tissue specimens after acute injury, and permits study of the balance between these events. The assessment of healthy and diseased cartilage and of the effects of surgical, pharmaceutical or in vitro intervention are immediate applications of these protocols. Moreover, this model may be useful for the study of key mechanisms involved in apoptosis or for the establishment of other markers of apoptosis.
The Pathologic Physiology of Nonunion (1965)

† From the Division of Orthopaedic Surgery, Western Reserve University School of Medicine and University Hospitals of Cleveland. ‡ 2073 Abington Rd., Cleveland, O.
Studies on the Bacteria Isolated from Wine (1969)

A malo-lactic fermentation (MLF) bacterium isolated from wine requires a factor contained in tomato juice for its growth. This type of the growth stimulating substance is found in several plants, particularly those belonging to fruits. The content of the factor in grapes varies depending on the varieties, degree of ripeness and harvest time. From these observation the relationship between the induction of MLF and the activity of the factor of grapes was discussed.
Molecular mechanisms involved in curcumol induced apoptosis in human gastric carcinoma cell line BGC823 (2013)

Objective To investigate the effect of curcumol on apoptosis of human gastric carcinoma cell line BGC823 and the molecular nechanisms.Methods BGC823 cells were cultured and treated with different curcumol concentration (12.5,25,50 and 100 mg/L) for 24 h and 48 h,and the growth inhibition were tested by thiazolyl blue terazolium bromide (MTF) assay.Flow cytometry (FCM) were used to measure the cell apoptosis rate and cell cycle of BGC823 cells.Caspase-3 activity was assessed by colorimetric assay.Cells treated with 100 mg/L curcunol for 48 h were collected and subjected to RTPCR and Western blot assays for the expression of Caspase-3,Bcl-2,Bax and Survivin.Results There was a time-and dose-dependent inhibition of cell proliferation of BGC823 cells by curcumol.Tbe cells in G0/G1 phase increased,and in S phase decreased on exposure to curcumol for 24 h.FCM analysis also indicated that the apoptosis rate of BGC823 cells increased in dose-dependent manner (P ＜ 0.05).Curcumol increased the activity of Caspase-3 dose-dependently (P ＜ 0.05).RT-PCR and Western blot indicated that curcumol decreased Bcl-2 and Survivin expression as well as increased Caspase-3 and Bax expression (P ＜ 0.05).Conclusions Curcumol inhibits BGC823 cell growth,arresting cells in G0/G1 phase and inducing cell apoptosis.The mechanism may be related with increasing the activity of Caspase-3,down-regulating the expression of Bcl-2 and Survivin,and up-regulating the expression of Caspase-3 and Bax.

Key words: 
Stomach neoplasms ;  Apoptosis ;  Cell proliferation;  Cell cycle
ChemInform Abstract: Synthesis of 11‐Aminodibenzo(b,f)(1,4)thiazepines and Fluoro Derivatives. (1997)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Investigation of Gyrate Atrophy Using a cDNA Clone for Human Ornithine Aminotransferase (1986)

Gyrate atrophy of the choroid and retina is an autosomal recessive disease associated with reduced or absent ornithine aminotransferase (OAT) activity. To approach the defect in OAT at the molecular level, we have cloned a cDNA for the mRNA encoding the OAT precursor from human liver. The clone contains the complete coding region of 1317 nucleotides along with 44 nucleotides of 5' and 654 nucleotides of 3' untranslated sequences. When used to probe genomic DNA, the OAT cDNA did not detect any evidence of gene deletion or rearrangement in patients with gyrate atrophy. The cDNA hybridizes to a 2.15-kb RNA species in liver, fibroblasts, and lymphoblasts. The size and approximate amount of this mRNA is not altered in fibroblasts and/or lymphoblasts of seven gyrate atrophy patients who display a 25- to 100-fold reduction in OAT activity. Our results suggest the defect in these individuals may be caused by a subtle sequence alteration in the mRNA that does not affect its apparent size.
Nanocomposite cross-linked conjugated polyelectrolyte/MWCNT/poly(pyrrole) for enhanced Mg2+ ion sensing and environmental remediation in real samples (2020)

Conjugated polyelectrolyte/MWCNT/poly(pyrrole) (CPE-K/MWCNT/PPY) composites have been prepared by a simple solution mixing technique. Synthesis of PPY/CPE/MWCNT composites was characterized by scanning electron microscopy and thermogravimetric analysis. These nanocomposites of CPE-K/MWCNT/PPY were deposited onto a glassy carbon electrode (GCE) in order to fabricate a reliable Mg2+ cationic sensor. A plot called the calibration curve is produced from the current as a function of concentration of Mg2+ ion. This plot was found to be linear in the range 0.1 nM to 0.01 mM, which is defined as the linear dynamic range. According to the surface area of the GCE (0.0316 cm2) and the slope of the calibration curve, a high sensitivity (19.4968 µA µM–1 cm–2) of the Mg2+ ion sensor was calculated. A significant lower limit of detection (97.71 ± 4.89 pM) was obtained in association with a signal to noise ratio of 3. Sensor parameters such as reproducibility, long-time stability, and short response time were measured and found to be quite satisfactory. This new approach to Mg2+ detection with high sensitivity is suggested for application as a novel multifunctional sensing system. Besides, it was found to be very effective to analyze real environmental samples.
Impact of Controlled Ni Contamination on Silicon Solar Wafer Material (2018)

Trace metal impurities are known to severely degrade the performance of silicon solar cells. The effect of Nickel dissolved in dilute cleaning solutions was studied in detail. Clean wafers were exposed to these mixtures.The resulting surface concentration and the effect on carrier lifetime upon thermal oxidation, were found to correlate with a first order relation to the concentration of Ni in the solution.The removal of Nickel from the surface by different cleaning recipes was evaluated and compared using thermal oxidation followed by carrier lifetime measurements.The resulting surface concentration and the effect on carrier lifetime upon thermal oxidation, were found to correlate with a first order relation to the concentration of Nickel in the solution.
<title>DEM integrity monitor experiment (DIME) flight test results</title> (2002)

This paper discusses flight test results of a Digital Elevation Model (DEM) integrity monitor. The DEM Integrity Monitor Experiment (DIME) was part of the NASA Synthetic Vision System (SVS) flight trials at Eagle-Vail, Colorado (EGE) in August/September, 2001. SVS provides pilots with either a Heads-down Display (HDD) or a Heads-up Display (HUD) containing aircraft state, guidance and navigation information, and a virtual depiction of the terrain as viewed 'from the cockpit'. SVS has the potential to improve flight safety by increasing the situational awareness (SA) in low to near zero-visibility conditions to a level of awareness similar to daytime clear-weather flying. This SA improvement not only enables low-visibility operations, but may also reduce the likelihood of Controlled Flight Into Terrain (CFIT). Because of the compelling nature of SVS displays high integrity requirements may be imposed on the various databases used to generate the imagery on the displays even when the target SVS application does not require an essential or flight-critical integrity level. DIME utilized external sensors (WAAS and radar altimeter) to independently generate a 'synthesized' terrain profile. A statistical assessment of the consistency between the synthesized profile and the profile as stored in the DEM provided a fault-detection capability. The paper will discuss the basic DIME principles and will show the DIME performance for a variety of approaches to Runways 7 and 25 at EGE. The monitored DEMs are DTED Level 0, USGS with a 3-arcsec spatial resolution, and a DEM provided by NASA Langley. The test aircraft was a Boeing 757-200.
Translesion Synthesis by Human DNA Polymerase κ on a DNA Template Containing a Single Stereoisomer of dG-(+)- or dG-(−)-<i>anti</i>-<i>N</i><sup>2</sup>-BPDE (7,8-Dihydroxy-<i>anti</i>-9,10-epoxy-7,8,9,10-tetrahydrobenzo[<i>a</i>]pyrene) (2002)

Several recently discovered human DNA polymerases are associated with translesion synthesis past DNA adducts. These include human DNA polymerase kappa (pol kappa), a homologue of Escherichia coli pol IV, which enhances the frequency of spontaneous mutation. Using a truncated form of pol kappa (pol kappa Delta C), translesion synthesis past dG-(+)- or dG-(-)-anti-N(2)-BPDE (7,8-dihydroxy-anti-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene) adducts was explored. Site-specifically-modified oligodeoxynucleotides containing a single stereoisomeric dG-N(2)-BPDE lesion were used as DNA templates for primer extension reactions catalyzed by pol kappa Delta C. Primer extension was retarded one base prior to the dG-N(2)-BPDE lesion; when incubated for longer times or with higher concentration of enzyme, full primer extension was observed. Quantitative analysis of fully extended products showed preferential incorporation of dCMP, the correct base, opposite all four stereoisomeric dG-N(2)-BPDE lesions. (+)-trans-dG-N(2)-BPDE, a major BPDE-DNA adduct, promoted small amounts of dTMP, dAMP, and dGMP misincorporation opposite the lesion (total 2.7% of the starting primers) and deletions (1.1%). Although (+)-cis-dG-N(2)-BPDE was most effective in blocking translesion synthesis, its miscoding properties were similar to other dG-N(2)-BPDE isomers. Steady-state kinetic data indicate that dCMP is efficiently inserted opposite all dG-N(2)-BPDE adducts and extended past these lesions. The relative frequency of translesion synthesis (F(ins) x F(ext)) of dC.dG-N(2)-BPDE pairs was 2-6 orders of magnitude higher than that of other mismatched pairs. Pol kappa may play an important role in translesion synthesis by incorporating preferentially the correct base opposite dG-N(2)-BPDE. Its relatively low contribution to mutagenicity suggests that other newly discovered DNA polymerase(s) may be involved in mutagenic events attributed to dG-N(2)-BPDE adducts in human cells.
<i>Pseudomonas aeruginosa</i> Contains a Novel Type V Porphobilinogen Synthase with No Required Catalytic Metal Ions<sup>†</sup> (1999)

Porphobilinogen synthases (PBGS) are metalloenzymes that catalyze the first common step in tetrapyrrole biosynthesis. The PBGS enzymes have previously been categorized into four types (I-IV) by the number of Zn(2+) and/or Mg(2+) utilized at three different metal binding sites termed A, B, and C. In this study Pseudomonas aeruginosa PBGS is found to bind only four Mg(2+) per octamer as determined by atomic absorption spectroscopy, in the presence or absence of substrate/product. This is the lowest number of bound metal ions yet found for PBGS where other enzymes bind 8-16 divalent ions. These four Mg(2+) allosterically stimulate a metal ion independent catalytic activity, in a fashion dependent upon both pH and K(+). The allosteric Mg(2+) of PBGS is located in metal binding site C, which is outside the active site. No evidence is found for metal binding to the potential high-affinity active site metal binding sites A and/or B. P. aeruginosa PBGS was investigated using Mn(2+) as an EPR probe for Mg(2+), and the active site was investigated using [3,5-(13)C]porphobilinogen as an NMR probe. The magnetic resonance data exclude the direct involvement of Mg(2+) in substrate binding and product formation. The combined data suggest that P. aeruginosa PBGS represents a new type V enzyme. Type V PBGS has the remarkable ability to synthesize porphobilinogen in a metal ion independent fashion. The total metal ion stoichiometry of only 4 per octamer suggests half-sites reactivity.
Duplex Saphenous Vein Mapping: Venous Occlusion and Dependent Position Facilitate Imaging (2002)

The purpose of this study was to develop an optimal technique for greater saphenous vein distention during preoperative duplex assessment. An Acuson 128 scanner with a 7.5-MHz sector probe was used to assess the effects of venous occlusion and dependent position on the diameter of the greater saphenous vein in 20 male volunteers. The greater saphenous vein was imaged 10 cm above and 10 cm below the knee with the subject lying horizontal, horizontal with a thigh tourniquet inflated to 40 mm Hg, inclined at 15 degrees of reversed Trendelenburg, and inclined at 15 degrees of reversed Trendelenburg combined with the tourniquet inflated to 40 mm Hg. Maximal vein diameters and circumferences were measured by a sonographer blinded to the conditions of vein measurement to avoid interpretation bias. Using a t test assuming equal variances, the increases in vein circumferences and diameters achieved by dependency and tourniquet combined were significantly greater than those achieved by dependency alone (p = 0.004). The technique of dependency combined with tourniquet inflation to 40 mm Hg facilitates greater saphenous vein imaging and provides an optimal method of venous distention.
Dynamic Tensile Strength of a Ferroelectric Ceramic (1961)

The dynamic tensile strength of a piezoelectric lead titanate zirconate ceramic used for power transducers was tested by driving small bars of ceramic electrically at their longitudinal resonance until they fractured. The measured dynamic tensile strength of small specimens of ceramic of good quality was found to be about 13 000 psi. This value was very drastically reduced for ceramic which was not of optimum quality.
Big lasers, tiny circuits (2010)

When it comes to computers, small is big. The more we are able to shrink the patterns of circuitry on semiconductor wafers, the smaller and more powerful the chips made from these wafers become.
Bosonic model of hole pairs (2001)

We numerically investigate a bosonic representation for hole pairs on a two-leg $t\ensuremath{-}J$ ladder where hard core bosons on a chain represent the hole pairs on the ladder. The interaction between hole pairs is obtained by fitting the density profile obtained with the effective model to the one obtained with the $t\ensuremath{-}J$ model, taking into account the inner structure of the hole pair given by the hole-hole correlation function. For these interactions we calculate the Luttinger liquid parameter, which takes the universal value ${K}_{\ensuremath{\rho}}=1$ as half filling is approached, for values of the rung exchange ${J}^{\ensuremath{'}}$ between strong coupling and the isotropic case. The long distance behavior of the hole-hole correlation function is also investigated. Starting from large ${J}^{\ensuremath{'}},$ the correlation length first increases as expected but diminishes significantly as ${J}^{\ensuremath{'}}$ is reduced and bound holes sit mainly on adjacent rungs. As the isotropic case is approached, the correlation length increases again. This effect is related to the different kind of bonds in the region between the two holes of a hole pair when they move apart.
The BiVACOR Rotary Biventricular Assist Device: Concept and In Vitro Investigation (2008)

The BiVACOR is a novel rotary Biventricular Assist Device (BiVAD) undergoing development to treat global end-stage heart failure. The design includes left and right vanes positioned on a shared rotating hub to form a double-sided magnetically and hydrodynamically suspended centrifugal impeller. The performance of the device was assessed in a pulsatile mock circulation loop replicating end-stage biventricular heart failure, and was shown to restore flow from pathological (2 L/min) to normal levels (5 L/min). A novel technique to balance the left/right outflow of the BiVAD was also investigated, for which a maximum relative left to right outflow differential of 1.8 L/min was achieved at normal physiologic afterloads. The in vitro results encourage device progression to in vivo animal studies. Successful development of this BiVAD will provide a suitably miniature device for patients who require biventricular assistance.
Magnetization measurements on (Ce,Gd)Al3 (1988)

Ce1−xGdxAl3 exhibits an interesting interplay of heavy-fermion, spin-glass, and antiferromagnetic behavior. Magnetization measurements were undertaken to further our understanding of this system and to estimate the fraction of the magnetization involved in the spin-glass and antiferromagnetic transitions. By making a linear extrapolation of the magnetization data to H=0, one defines the spontaneous magnetization, Ms. At T=2 K, we find that Ms is approximately proportional to x for x≤0.4 and equal to approximately 10% of the maximum possible contribution from just the Gd moments. Ms increases at a much faster rate between x=0.40 and 0.50, but then decreases to zero at x=0.635 where the system is antiferromagnetic. For x=0.635 and T=1.5 K, the magnetization is proportional to H for H≤80 kOe. Using this linearity, we suggest that nearly all the spins are correlated at low temperatures for x≥0.635.
Zero Skew Differential Clock Distribution Network (2006)

Clock uncertainty is a major concern in current high performance clock network design. A differential clocking scheme provides noise immunity and can address this challenge. In this paper, a differential line equivalent delay model is proposed to obtain zero skew differential clock networks. The method is applied to various benchmarks. On average, 97% skew reduction is obtained compared to the solution derived with the classic Elmore model. To improve performance of zero skew differential clock networks, differential buffers based on dynamic threshold transistors are proposed. Incorporation of proposed buffers to low swing zero skew differential clock network shows 25% delay improvement compared to conventional buffers. Moreover, the incorporation of proposed design methods shows 25% and 6% skew variations reduction in presence of power supply variations and crosstalk noise respectively, compared to low swing single-node clock distribution networks.
Development of drug loaded cardiovascular prosthesis for thrombosis prevention using 3D printing (2021)

Cardiovascular disease (CVD) is a general term for conditions which are the leading cause of death in the world. Quick restoration of tissue perfusion is a key factor to combat these diseases and improve the quality and duration of patients' life. Revascularization techniques include angioplasty, placement of a stent, or surgical bypass grafting. For the latter technique, autologous vessels remain the best clinical option; however, many patients lack suitable autogenous due to previous operations and they are often unsuitable. Therefore, synthetic vascular grafts providing antithrombosis, neointimal hyperplasia inhibition and fast endothelialization are still needed. To address these limitations, 3D printed dipyridamole (DIP) loaded biodegradable vascular grafts were developed. Polycaprolactone (PCL) and DIP were successfully mixed without solvents and then vascular grafts were 3D printed. A mixture of high and low molecular weight PCL was used to better ensure the integration of DIP, which would offer the biological functions required above. Moreover, 3D printing technology provides the ability to fabricate structures of precise geometries from a 3D model, enabling to customize the vascular grafts' shape or size. The produced vascular grafts were fully characterized through multiple techniques and the last step was to evaluate their drug release, antiplatelet effect and cytocompatibility. The results suggested that DIP was properly mixed and integrated within the PCL matrix. Moreover, these materials can provide a sustained and linear drug release without any obvious burst release, or any faster initial release rates for 30 days. Compared to PCL alone, a clear reduced platelet deposition in all the DIP-loaded vascular grafts was evidenced. The hemolysis percentage of both materials PCL alone and PCL containing 20% DIP were lower than 4%. Moreover, PCL and 20% DIP loaded grafts were able to provide a supportive environment for cellular attachment, viability, and growth.
DNA sequence analysis of diuron‐resistant mutations in the mitochondrial cytochrome <i>b</i> gene of <i>Saccharomyces cerevisiae</i> (1986)

Diuron (3-[3,4-dichlorophenyl]-1,1-dimethylurea), an inhibitor of mitochondrial respiration, blocks the yeast respiratory chain between cytochrome b and c1. Diuron-resistant mutants of Saccharomyces cerevisiae have been selected and several mutations localized to the mitochondrial cytochrome b gene. The present paper identifies specific DNA base changes within the cytochrome b gene conferring diuron-resistance. DNA sequence analysis was done utilizing primer extension of crude mitochondrial RNA preparations in the presence of reverse transcriptase. Five independent diuron-resistant mutations have been sequenced.
Morphometric Evaluation of the Cervico-Thoracic Junction (1994)

Because of the complexities of the cervico-thoracic junction, additional characterization increases the knowledge of the anatomic relationships in this region.