gebhart/patrepeval huggingface dataset:
Effects of Ca2+ and calmodulin on contraction of chemically skinned muscle fibers from pregnant rat uteri. (1986)

The effects of Ca2+ and calmodulin on contraction of saponin-treated (chemically skinned) uterine smooth muscle fibers of pregnant rats were examined. Ca2+ sensitivity, defined as the pCa required for half maximum activation of force production, was found to change with the progress of pregnancy; low in the early and middle stages and high in the later stages of pregnancy. The overall change of Ca2+ sensitivity was about pCa 1.5 during the period of pregnancy. The effect of calmodulin on contraction was also found to be dependent on the stages of pregnancy. Calmodulin was effective on the augmentation of the tension rather than the change in Ca2+ sensitivity, and this augmentation was large in the early and middle stages of pregnancy. The amount of calmodulin, which eluted out of uterine muscle cells during saponin treatment, was large in the early and middle stages of pregnancy. The results indicate that the contractile response of the uterine muscle cells during the period of pregnancy seems to be controlled by both the changes in Ca2+ sensitivity and in the amount of free calmodulin in uterine muscle cells.
AMELIE accelerates Mendelian patient diagnosis directly from the primary literature (2017)

Abstract The diagnosis of Mendelian disorders requires labor-intensive literature research. Our software system AMELIE ( A utomatic M endelian L iterature E valuation) greatly automates this process. AMELIE parses hundreds of thousands of full text articles to find an underlying diagnosis to explain a patient’s phenotypes given the patient’s exome. AMELIE prioritizes patient candidate genes for their likelihood of causing the patient’s phenotypes. Diagnosis of singleton patients (without relatives’ exomes) is the most time-consuming scenario. AMELIE’s gene ranking method was tested on 215 singleton Mendelian patients with a clinical diagnosis. AMELIE ranked the causal gene among the top 2 in the majority (63%) of cases. Examining AMELIE’s top 10 genes, amounting to 8% of 124 candidate genes with rare functional variants per patient, results in diagnosis for 95% of cases. Strikingly, training only on gene pathogenicity knowledge from 2011 leads to identical performance compared to training on current data. An accompanying analysis web portal has launched at AMELIE.stanford.edu .
Beta-blockade with nebivolol enhances the acetylcholine-induced vasodilation in the cutaneaous vascular bed of normotensive volunteers (2001)

This study was undertaken to assess whether the beta1-adrenoceptor blocker nebivolol(N) increases the vasodilatory response to acetylcholine(Ach) when administered orally to healthy subjects. To this end 12 volunteers were randomly allocated to a 8-day treatment with nebivolol (n), 5 mg once a day, and atenolol(A),50 mg once a day, according to a cross-over design, with a 1 week wash-out period between the two treatment phases. The forearm skin blood flow(SBF) response to Ach applied by iontophoresis was determined using a laser-Doppler scanner imaging system before(T0) and 3 hours(T3) after N or A dosing, both on the first (Day 1) and the last day (Day 8) of treatment. The following Table shows the responses of SBF (perfusion units) (means±SD; *p<0.05 versus T0): Iontophoresis of 0.09% NaCl had no effect on SBF. These data indicate that nebivolol (administered at a dose commonly used in clinical practice), but not atenolol, enhances in humans the vasorelaxant activity of Ach in the skin vascular bed, which is compatible with a facilitation by this beta-blocker of the endothelium-dependent vasodilation.
Effect of seed preparation on efficiency and oil quality in filtration extraction of rapeseed (1964)

Abstract The application of filtration extraction to rapeseed is discussed with particular emphasis on the effect of seed preparation on the hydrogenation characteristics of the oil. It was found that cooking the crushed seed without the addition of water, and at temperatures not exceeding 220F, produced a satisfactory oil. Under these conditions extraction efficiency was good and the resulting meal showed no harmful effects in feeding trials with mice.
Hepatobiliary damage and changes in hepatic gene expression caused by the antitumor drug ecteinascidin-743 (ET-743) in the female rat. (2002)

Ecteinascidin-743 (ET-743) is a novel marine-derived anticancer drug with clinical activity in soft tissue sarcoma and ovarian cancer. Reversible transaminitis and subclinical cholangitis have frequently been described in patients who receive ET-743. To facilitate understanding of this adverse effect and help design suitable therapeutic rescue strategies, we characterized the hepatic effects of ET-743 in rats. Female rats received ET-743 (single dose, 40 microg/kg) i.v., and liver changes were assessed from 6 h up to 3 months after dosing by histopathology, immunohistochemistry, electron microscopy, hepatic and plasma biochemistry, and DNA microarray analysis. At 24 h posttreatment and beyond, livers displayed degeneration and patchy focal necrosis of bile duct epithelial cells associated with mild inflammation followed by fibrosis. Sporadic and focal zones of hepatic necrosis and hemorrhage were observed from day 2 onward, although the majority of hepatocytes appeared normal as judged by electron microscopy. Pathological alterations persisted up to 3 months after dosing. Plasma levels of total bilirubin were elevated up to 7-fold over those in untreated rats from day 2 onward and returned to control values by day 24. Activities of alkaline phosphatase and aspartate aminotransferase in plasma were elevated for 2 and 3 months, respectively. Activities of the hepatic microsomal drug-metabolizing enzymes cytochrome P-450 A1/2, CYP2E1, and CYP3A2 were decreased. DNA microarray analysis of livers from ET-743-treated animals showed a dramatic increase in the expression of ATP binding cassette transport genes Abcb1a and Abcb1b, which impart resistance to anticancer drugs, and of Cdc2a and Ccnd1, the rodent homologues of human cell cycle genes CDC2 and cyclin D1, respectively. The cell cycle gene expression changes mirrored ET-743-induced increases in liver weight and Ki-67 labeling of liver nuclei. The results suggest that the toxicity exerted by ET-743 in the rat liver is a consequence of biliary rather than hepatocellular damage and that it is accompanied by a wave of mitogenic activity, which may be driven by the transcriptional increase in Cdc2a expression.
Time-resolved LIBS experiment for quantitative determination of pollutant concentrations in air (1991)

We propose a new time-resolved laser-induced breakdown spectroscopy (TRELIBS) system for quantitative determination of small amounts of pollutants in gas. Some experimental results showing the high resolution of the method are reported, and possible improvements of the system are discussed.
System Identification of an Unmanned Aerial Vehicle with Hingeless Control Effectors (2010)

This paper presents research conducted for system identi cation of an unmanned aerial vehicle to quantitatively compare control authority of conventional ailerons and jet actuators in lateral/directional motion. Locally linear state-space models were identi ed, via the Observer/Kalman lter algorithm, to reveal the dynamics of the unmanned aerial vehicle test-bed for di erent roll control e ector con gurations. The accuracy of the generated models is demonstrated by examination of residual error between the recorded ight states and the identi ed state-space model output using the recorded pilot inputs. Linear systems theory is used to (1) identify correct linearized model order for minimal realization of the equations of motion, (2) verify consistent plant dynamics across roll e ector con gurations, and (3) quantitatively assess roll e ector authority across the con gurations. The identi ed system models yield a quantitative comparison of control e ectiveness of conventional and proposed aileron actuators which align with pilot feedback and test observations.
CLONING OF THE PORCINE COSTIMULATORY MOLECULE CD40. (1998)

460 Direct antigen presentation by porcine lymphocytes and endothelial cells to human T cells requires the appropriate interaction of porcine costimulatory molecules with their human counterreceptors on the T cell. We have isolated the gene for the porcine costimulatory molecule CD40 in order to further characterize its interaction with human T cells. We designed degenerate CD40 primers based on homology between the published mouse and human sequences. RNA was isolated from porcine splenocytes stimulated with TPA and forskolin, reverse transcribed and amplified by polymerase chain reaction(PCR) using the degenerate primers. The 5' and 3' ends of the porcine CD40 gene were isolated using rapid amplification of cDNA ends (RACE) and were sequenced using standard dideoxy sequencing. Porcine CD40 has an open reading frame of 836 base pairs corresponding to a protein of 279 amino acids. Comparison of the pig and human CD40 sequences revealed 82% homology at the amino acid level. A search of all deposited genbank sequences revealed that porcine CD40 was closest in homology to the human CD40. Semiquantitative RTPCR was used to analyze the level of CD40 mRNA in different tissues. CD40 mRNA was detected in TPA stimulated splenocytes and faintly in unstimulated splenocytes. Porcine aortic endothelial cells (PAEC) passaged twice expressed significant levels of CD40 mRNA. We have isolated the porcine CD40 gene. The porcine gene is highly homologous to human CD40 gene and is expressed in endothelial cells. This will allow us to study the interactions between swine CD40 and human CD40L in direct costimulation of T cells and endothelial activation.
Microengineered iron and iron-oxide contrast agent particles for tunable multispectral magnetic resonance imaging (2011)

In this paper, we demonstrate a novel type of contrast agent with multiplexing capability for use in magnetic resonance imaging (MRI) applications. Keeping biocompatibility in mind, iron and several iron oxide thin films with varying magnetic properties were prepared and characterized using a superconducting quantum interference device (SQUID). Double-disk shaped particles constructed with iron and iron oxide disks separated by polyimide posts were fabricated and validated using an MRI scanner. Spectral tunability was achieved by varying both the geometry and the magnetic properties of the particles.
Effect of suspended sediment on fertilization success in the scleractinian coral <i>Pectinia lactuca</i> (2012)

The effect of increased levels of suspended sediment on fertilization success in the scleractinian coral Pectinia lactuca was investigated in a laboratory experiment following a mass coral spawning event on reefs off Singapore. Egg–sperm bundles were collected from tank-spawned coral colonies collected from the field several days prior to the anticipated mass spawning. Eggs and sperm from each colony were separated and distributed systematically across replicated treatments (N = 9) with three concentrations of fine suspended sediment. Spawning and embryo development in Pectinia lactuca followed a pattern similar to other scleractinian coral species. There was a significant effect of increased suspended sediment concentration on fertilization success ( P < 0.05). Both high- (169 mg l −1 ) and medium- (43 mg l −1 ) suspended sediment treatments decreased fertilization success compared to controls. These results imply that increased turbidity levels (whether chronic, such as in the waters around Singapore, or short-term, caused by a dredging operation)—when coinciding with the coral spawning season—may affect the reproductive success of corals and compromise coral recruitment and recovery of degraded reefs.
A.C. small-signal model for bipolar transistors in saturation (1971)

A modified charge-control theory is used to derive a small-signal equivalent circuit which is valid in saturation. From this circuit can be deduced the fT(Ic) and the transconductance gfb(Ie), for example, which are compared with measurements. A short discussion of the results is given.
Synthesis and Secretion of Glycoprotein by the Epididymal Epithelium (1983)

The secretory activity of the middle segment of the mouse caput epididymidis was studied using 3H-fucose as a precursor to glycoprotein. Young adult male mice were injected with a concentrated solution of 3H-fucose interstitially, ie beneath the connective tissue capsule of the epididymis. Two animals were killed and prepared for light microscopic radioautography at each of six intervals between 10 minutes and 24 hours after injection. Silver grains were concentrated over the supranuclear Golgi region at 10 minutes and over the apical ends of the cells 30 minutes and 1 hour after injection. Quantitative analysis showed that luminal radioactivity increased greatly beginning with the 2-hour samples. The results indicate that the epididymal epithelium synthesizes and secretes glycoproteins, and that 1 to 2 hours are required for terminal glycosylation, intracellular transport, and release of the secretory product.
Studies of engine-airframe integration on hypersonic aircraft (1970)

Airframe-propulsion system integration for Mach 6 transport and Mach 12 research airplane, examining off-design operation effects and interaction of aerodynamic forces
Bidirectional hybrid fiber amplifiers in a recycling-pump mechanism (2012)

In this paper, a bidirectional hybrid fiber amplifiers is proposed and experimentally demonstrated. The hybrid amplifiers include a C-band erbium-doped fiber amplifier (EDFA) and an L-band Raman fiber amplifier (RFA). A single-wavelength pump source at 1495 nm is pumped shared to both the EDFA and the RFA. Under the pump power of 500 mW, the average gains are about 11.3 dB for the C band EDFA and 10.0 dB for the L band RFA, respectively, with a launched signal power of -10 dBm per channel. For 50 km and 75 km bidirectional transmission, the power penalties are less than 0.38 dB and 0.88 dB, respectively, as compared to their unidirectional transmissions.
Fabrication and Enhanced Thermoelectric Properties of Alumina Nanoparticle‐Dispersed Bi<sub>0.5</sub>Sb<sub>1.5</sub>Te<sub>3</sub> Matrix Composites (2013)

Alumina nanoparticle‐dispersed bismuth‐antimony‐tellurium matrix (Al 2 O 3 /BST) composite powders were fabricated by using ball milling process of alumina nanoparticle about 10 nm and p‐type bismuth telluride nanopowders prepared from the mechanochemical process (MCP). The fabricated Al 2 O 3 /BST composite powders were a few hundreds of nanometer in size, with a clear Bi 0.5 Sb 1.5 Te 3 phase. The composite powders were consolidated into p‐type bulk composite by spark plasma sintering process. High‐resolution TEM images reveal that alumina nanoparticles were dispersed among the grain boundary or in the matrix grain. The sintered 0.3 vol.% Al 2 O 3 /BST composite exhibited significantly improved power factor and reduced thermal conductivity in the temperature ranging from 293 to 473 K compared to those of pure BST. From these results, the highly increased ZT value of 1.5 was obtained from 0.3 vol.% Al 2 O 3 /BST composite at 323 K.
Inhibition of Settlement by Larvae of <i>Balanus amphitrite</i> and <i>Ciona intestinalis</i> by a Surface-Colonizing Marine Bacterium (1992)

In an attempt to isolate bacteria with inhibitory effects against settlement by larvae of sessile invertebrates, 40 marine bacterial isolates were screened for effects against laboratory-reared barnacle larvae (Balanus amphitrite) and ascidian larvae (Ciona intestinalis). Five isolates displayed non-pH-dependent inhibitory effects against the larvae. The initial characterization of a toxic component released from an isolate, designated D2 (CCUG 26757), and its effect on laboratory-reared barnacle and ascidian larvae were studied. D2 is a facultative, anaerobic, gram-negative bacterium isolated from the surface of C. intestinalis from waters off the Swedish west coast at a depth of 10 m. Results suggest that the toxic component is released by D2 during the stationary phase. Aged biofilms were more toxic to the larvae than unaged films. The biologically active compound was in the supernatant of D2 and was heat stable and <500 Da in molecular mass. No evidence of protein or peptide moieties was found. On the basis of two phase and chromatography separations, the component is polar and neutral and contains or binds to carbohydrate moieties. Metaperiodate treatment increased toxicity; undiluted supernatant from a 24-h growth culture of D2 killed barnacle and ascidian larvae within a few hours of exposure, whereas after metaperiodate treatment, the larvae were killed in approximately 30 min.
ChemInform Abstract: Directed Intermolecular Carbomagnesation Across Vinylsilanes: 2‐PyMe<sub>2</sub>Si Group as a Removable Directing Group. (2001)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
HC2091 Kills Mycobacterium tuberculosis by Targeting the MmpL3 Mycolic Acid Transporter (2018)

Tuberculosis, caused by the intracellular pathogen <i>Mycobacterium tuberculosis</i>, is a deadly disease that requires a long course of treatment. The emergence of drug-resistant strains has driven efforts to discover new small molecules that can kill the bacterium. Here, we report characterizations of the compound HC2091, which kills <i>M. tuberculosis</i> in a time- and dose-dependent manner <i>in vitro</i> and inhibits <i>M. tuberculosis</i> growth in macrophages. Whole-genome sequencing of spontaneous HC2091-resistant mutants identified single-nucleotide variants in the <i>mmpL3</i> mycolic acid transporter gene. HC2091-resistant mutants do not exhibit cross-resistance with the well-characterized <i>Mycobacterium</i> membrane protein large 3 (MmpL3) inhibitor SQ109, suggesting a distinct mechanism of interaction with MmpL3. Additionally, HC2091 does not modulate bacterial membrane potential or kill nonreplicating <i>M. tuberculosis</i>, thus acting differently from other known MmpL3 inhibitors. RNA sequencing (RNA-seq) transcriptional profiling and lipid profiling of <i>M. tuberculosis</i> treated with HC2091 or SQ109 show that the two compounds target a similar pathway. HC2091 has a chemical structure dissimilar to those of previously described MmpL3 inhibitors, supporting the notion that HC2091 is a new class of MmpL3 inhibitor.
Molecular gas in the Galactic center region (1997)

A large scale survey of the Galactic center region in the () transition is presented. This survey was obtained with the 1.2 m Southern Millimeter-Wave Telescope (SMWT) at the Cerro Tololo Interamerican Observatory (CTIO) near La Serena, Chile. It covers the region and with a grid spacing of 9, i.e. the sampling is at full FWHP beamwidth. 357 positions were in total observed. After reviewing the instrumentation of the 1.2 m SMWT, the observing techniques, and the methods used in the data reduction, the data of the survey are presented and morphologically described. In addition, data of the HNCO() line are presented, which was also included in the large bandwidth of the spectrometer. measurements performed for comparison purposes are presented and compared with other 12CO results. The maps of the survey demonstrate that there are great differences between the distribution of the optically thin emission and the usually optically thick emission.
Anti-cancer effect of Annona Muricata Linn Leaves Crude Extract (AMCE) on breast cancer cell line (2016)

The results suggest that, B1 AMCE is a promising candidate for cancer treatment especially in breast cancer and deserves further research as an alternative to conventional drugs while also stressed out the selection of soursop sample which plays a significant role in determining its potential therapeutic effect on cancer.
Semantic Feedback for Hybrid Recommendations in Recommendz (2005)

In this paper we discuss the Recommendz recommender system. This domain-independent system combines the advantages of collaborative and content-based filtering in a novel way. By allowing users to provide feedback not only about an item as a whole, but also properties of an item that motivated their opinion, increased performance seems to be achieved. The features used to describe items are specified by the users of the system rather than predetermined using manual knowledge-engineering. We describe a method for combining descriptive features and simple ratings, and provide a performance analysis.
Nucleophilic Addition to<i>N</i>-Methoxyamides via<i>N</i>-Oxyiminium Ions (2010)

This work reports a nucleophilic ­addition to N-alkoxyamides in a sequential one-pot procedure that does not require preactivation of the amide. The reaction proceeds well for ­acyclic amides and macrocycles. Even an intra­molecular allylation of an acyclic N-methoxyamide was successful.
Polarity-inverted ScAlN film growth by ion beam irradiation and application to overtone acoustic wave (000-1)/(0001) film resonators (2014)

Polarity inversion in wurtzite film is generally achieved by the epitaxial growth on a specific under-layer. We demonstrate polarity inversion of c-axis oriented ScAlN films by substrate ion beam irradiation without using buffer layer. Substrate ion beam irradiation was induced by either sputtering a small amount of oxide (as a negative ion source) onto the cathode or by applying a RF bias to the substrate. Polarity of the films was determined by a press test and nonlinear dielectric measurement. Second overtone thickness extensional mode acoustic resonance and suppression of fundamental mode resonance, indicating complete polarity inversion, were clearly observed in bilayer highly oriented (000-1)/(0001) ScAlN film.
Immunochemical Specificity of Lactate Di (2016)

Sperm-producing testes of mammals and some birds contain a unique form of lactate dehydrogenase (EC 1.1.1.27) which is a tetramer composed of C subunits, rather than the A and B subunits of lactate dehydrogenase isozymes 1-5. Immunochemical evidence is presented which confirms the hypothesis that the C subunit is encoded in a separate gene from the A and B polypeptides. The evolutionary relationships of these genes are discussed. Studies by many investigators have clearly established that the five major types of lactate dehydrogenase (LDH; EC 1.1.1.27) in mammals are formed by the random combination of two different subunits into tetramers. Each subunit (A and B) is the product of a distinct gene locus (1). There is now substantial evidence that the isozyme LDH-X of the sperm cell is also a tetramer and that it is composed of a third poly- peptide type (C), which is encoded in a separate gene (2-5). Apparently this gene is active only during the primary spermatocyte stage of the spermatogenic process and is in- active in all other cells of the organism (6, 7). Immunological procedures have proved to be potent tools for establishing subunit homologies (8) and for examining the evolution of lactate dehydrogenases (9). The selective in- hibiting and precipitating activities of antibodies have been used in the present investigation to demonstrate that the C subunit of LDH-X is indeed distinct from the A and B poly- peptide, thus confirming its independent genetic control, and further, to show that this isozyme from several vertebrate species has a common evolutionary origin. METHODS