gebhart/patrepeval huggingface dataset:
Investigation of the conformational equilibrium of 1-methylphosphorinane by low-temperature phosphorus-31 and proton nuclear magnetic resonance spectroscopy (1973)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTInvestigation of the conformational equilibrium of 1-methylphosphorinane by low-temperature phosphorus-31 and proton nuclear magnetic resonance spectroscopySidney I. Featherman and Louis D. QuinCite this: J. Am. Chem. Soc. 1973, 95, 5, 1699–1700Publication Date (Print):March 1, 1973Publication History Published online1 May 2002Published inissue 1 March 1973https://pubs.acs.org/doi/10.1021/ja00786a077https://doi.org/10.1021/ja00786a077research-articleACS PublicationsRequest reuse permissionsArticle Views53Altmetric-Citations19LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Cell-Free Spent Media Obtained from Bifidobacterium bifidum and Bifidobacterium crudilactis Grown in Media Supplemented with 3′-Sialyllactose Modulate Virulence Gene Expression in Escherichia coli O157:H7 and Salmonella Typhimurium (2016)

Complex oligosaccharides from human milk (HMO) possess an antimicrobial activity and can promote the growth of bifidobacteria such as <i>Bifidobacterium bifidum</i> and <i>Bifidobacterium longum</i> subsp. <i>infantis</i>. In addition, fermentation of carbohydrates by bifidobacteria can result in the production of metabolites presenting an antivirulence effect on several pathogenic bacteria. Whey is rich in complex bovine milk oligosaccharides (BMO) structurally similar to HMO and <i>B. crudilactis</i>, a species of bovine origin, is able to metabolize some of those complex carbohydrates. This study focused on the ability of <i>B. bifidum</i> and <i>B. crudilactis</i> to grow in a culture medium supplemented in 3'-sialyllactose (3'SL) as the main source of carbon, a major BMO encountered in cow milk. Next, the effects of cell-free spent media (CFSM) were tested against virulence expression of <i>Escherichia coli</i> O157:H7 and <i>Salmonella enterica</i> serovar Typhimurium. Both strains were able to grow in presence of 3'SL, but <i>B. crudilactis</i> showed the best growth (7.92 ± 0.3 log cfu/ml) compared to <i>B. bifidum</i> (6.84 ± 0.9 log cfu/ml). Then, CFSM were tested for their effects on virulence gene expression by <i>ler</i> and <i>hilA</i> promoter activity of luminescent mutants of <i>E. coli</i> and <i>S</i>. Typhimurium, respectively, and on wild type strains of <i>E. coli</i> O157:H7 and <i>S</i>. Typhimurium using RT-qPCR. All CFSM resulted in significant under expression of the <i>ler</i> and <i>hilA</i> genes for the luminescent mutants and <i>ler</i> (ratios of -15.4 and -8.1 respectively) and <i>qseA</i> (ratios of -2.1 and -3.1) for the wild type strain of <i>E. coli</i> O157:H7. The 3'SL, a major BMO, combined with some bifidobacteria strains of bovine or human origin could therefore be an interesting synbiotic to maintain or restore the intestinal health of young children. These effects observed <i>in vitro</i> will be further investigated regarding the overall phenotype of pathogenic agents and the exact nature of the active molecules.
The receptor activator of <scp>NF</scp>‐κ<scp>B</scp> ligand‐osteoprotegerin system in pulpal and periapical disease (2012)

There is relatively little information currently available that would highlight the specific role of RANKL and OPG in pulpal and periapical disease. OPG may play a protective role against internal resorption, whilst an increased periapical RANKL/OPG ratio might indicate bone resorption.
Die dynamische Struktur von Tau‐Filamenten (2011)

Einblicke in ein komplexes Netzwerk: Die dynamische Struktur des „ungeordneten Mantels“ („Fuzzy Coat“), N- und C-terminaler Abschnitte um den Kern des Tau-Filaments bestehend aus über 200 Aminosäuren, wurde NMR-spektroskopisch charakterisiert (siehe Modell des Netzwerks langreichweitiger Wechselwirkungen). Die Ergebnisse liefern zudem Erkenntnisse über die Bindung von konformationsspezifischen Antikörpern an das Tau-Protein. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Construction of a Stapled α-Helix Peptide Library Displayed on Phage for the Screening of Galectin-3-Binding Peptide Ligands (2020)

A stapled α-helix peptide library was designed and constructed using a chemically modified phage display system for screening stapled-peptide ligands against target proteins. The α-helix peptide library, with two cysteine residues on the opposite side of the randomized face, was modified with a rigid hydrocarbon staple linker on a phage. The stapled α-helix peptide phage library was screened against galectin-3 (Gal-3), a cancer-related galactose-binding protein. The obtained stapled peptides showed a high binding affinity (<i>K</i> <sub>d</sub> = 0.45 μM) despite being nonsugar ligands. The stapled modification played important roles in stabilizing the α-helical structure that contributed to the high binding affinity to Gal-3. In addition, the best stapled peptide ligands showed specific binding to Gal-3 among various carbohydrate-binding proteins. Thus, the designed α-helix peptide phage library with a constrained structure by the staple linker will advance the discovery of peptide ligands with improved specificity and affinity.
Development of a Class-Specific Competitive ELISA for the Benzoylphenylurea Insecticides (1998)

A competitive enzyme-linked immunosorbert assay (ELISA) was developed for the quantitative detection of five benzophenylurea insecticides in soil (90% methanol extraction) and water. No significant matrix effects were observed. Conjugates of diflubenzuron derivatives with proteins were prepared by attachment at either the urea nitrogen of diflubenzuron or the 4-position of the aniline ring, wherein bridging groups of various lengths were used between the hapten and the protein carrier. All combinations of antiserum to each immunogen and enzyme tracer were examined, and an assay was developed using an antibody developed to a hapten conjugate coupled through a succinyl chain at the 4-aniline position. The assay gave 50% inhibition of antibody binding (IC50) at 0.6 ppb for diflubenzuron, 5 ppb for teflubenzuron, 10 ppb for flufenoxuron, 31 ppb for lufenuron, and 45 ppb for chlorfluazuron, with detection limits in the range of 0.05−2.3 ppb for these compounds. The basis for the superior cross-reaction of diflubenzuron was examined by molecular modeling, which suggested that the planarity of the molecules, electron-withdrawing groups, and steric effects of chlorines attached to the phenyl ring may be critical factors affecting antibody binding. Keywords: Benzoylphenylurea; polyclonal antibody; ELISA; molecular modeling
Effects of Graded Doses of Testosterone on Erythropoiesis in Healthy Young and Older Men (2007)

Testosterone has a dose-dependent stimulatory effect on erythropoiesis in men that is more pronounced in older men. The testosterone-induced rise in hemoglobin and hematocrit and age-related differences in response to testosterone therapy may be mediated by factors other than erythropoietin and sTfR.
Conditions for Melt Crystallization Without Thermal Degradation and Equilibrium Melting Temperature of Atactic Poly(Vinyl Alcohol) (2003)

Optimum conditions for melt crystallization without thermal degradation for atactic poly(vinyl alcohol) (PVA) were found for the first time. Thermal degradation of PVA in the melt has been too serious to permit crystallization from the melt. However, it was found that thermal degradation can be suppressed by selecting an appropriate solvent for film preparation, such as water without dimethyl sulfoxide (DMSO), by careful degassing before melting and by melting under high vacuum. As a result, the sample could be held in the melt at 250°C for 30 min and in the supercooled melt at 220°C for 480 min without thermal degradation. Therefore we could carry out the melt crystallization at a high crystallization temperature (T c) in the range of 208 to 221°C and study the structures and morphologies of melt-crystallized PVA samples for the first time. The irregularly stacked lamellae were observed irrespective of T c. The thick lamellae with high T m, 24 nm and 236.1°C, respectively, were obtained from the melt crystallization at T c=220°C, which indicates that the chain sliding diffusion is accelerated a little with increase of T c. However, the distribution of lamellar thickness was sharp, even for the sample crystallized at high T c, which indicated that the chain sliding diffusion is relatively suppressed by the intermolecular hydrogen bonds. Hence, the lamellar thickening was suppressed during the crystallization, as compared with polyethylene (PE) and isotactic polypropylene (iPP). The equilibrium melting temperature of PVA was determined to be 249°C, applying an improved Gibbs–Thomson plot that was recently proposed by Yamada et al. The Hoffman–Weeks plot accidentally gave a similar It was confirmed that the lamellar thickness is inversely proportional to the degree of supercooling in the case of PVA crystals, which is the reason why both plots gave similar
Redox Sensitive Cysteine Residues in Calbindin D<sub>28k</sub>Are Structurally and Functionally Important (2004)

Human calbindin D(28k) is a Ca(2+) binding protein that has been implicated in the protection of cells against apoptosis. In this study, the structural and functional significance of the five cysteine residues present in this protein have been investigated through a series of cysteine-to-serine mutations. The mutants were studied under relevant physiological redox potentials in which conformational changes were monitored using ANS binding. Urea-induced denaturations, as monitored by intrinsic tryptophan fluorescence, were also carried out to compare their relative stability. It was shown that the two N-terminal cysteine residues undergo a redox-driven structural change consistent with disulfide bond formation. The other cysteine residues are not by themselves sufficient at inducing structural change, but they accentuate the disulfide-dependent conformational change in a redox-dependent manner. Mass spectrometry data show that the three C-terminal cysteine residues can be modified by glutathione. Furthermore, under oxidizing conditions, the data display additional species consistent with the conversion of cysteine thiols to sulfenic acids and disulfides to disulfide-S-monoxides. The biological function of calbindin D(28k) appears to be tied to the redox state of the cysteine residues. The two N-terminal cysteine residues are required for activation of myo-inositol monophosphatase, and enzyme activation is enhanced under conditions in which these residues are oxidized. Last, oxidized calbindin D(28k) binds Ca(2+) with lower affinity than does the reduced protein.
Short term clodronate treatment does not increase trabecular bone in posttraumatic osteopenia. (1995)

The paper describes the results of two experiments on clodronate treatment on bone metabolism in growing rabbits: 1. the serum and bone concentrations of the drug, and 2. the effects of 18 week clodronate treatment on quantitative histomorphometry of trabecular bone. The results indicated a rapid and high affinity of clodronate to bone after subcutaneous administration. Plate fixation of the tibia without a fracture or any operation on the femur induced a 25% decrease in bone volume per total volume in the femoral condyles. The osteopenic response resulted probably from operative trauma. Clodronate treatment for three months could not inhibit this decrease. The increase in calcium content in bone by the drug treatment (observed in diaphyseal bones in earlier studies) could not be verified on histology of trabecular bone.
Abstract LB-498: Density of tertiary lymphoid structures is associated with activated and effector-memory T lymphocyte infiltration in human lung tumor (2012)

Abstract Introduction: An increasing number of studies have demonstrated a strong correlation between T cell infiltrate and clinical outcome in several types of human solid cancers. Nevertheless, the mechanisms governing T-cell infiltration and activation into tumors remain poorly characterized. In lung cancer, our team has observed the presence of tertiary lymphoid structures called Ti-BALT (Tumor-induced Bronchus-Associated Lymphoid Tissues). These structures present features of an ongoing immune response and their density is associated with long-term survival for lung cancer patients, suggesting their implication in local T cell recruitment and activation. We recently published a specific gene expression signature associated with T cell presence in Ti-BALT, which includes lymphoid chemokines, adhesion molecules, and integrins (De Chaisemartin L, et al. Cancer Research 2011). This chemoattractant gene expression signature strongly suggests an active recruitment of immune cells in these structures. Our aim was to study the influence of these structures on the composition, density and functionality of the immune infiltrate in lung tumors. Methods: The expression of relevant molecules was assessed on fresh tumor-infiltrating lymphocytes by multicolor flow cytometry, on tissue sections by immunohistochemistry and on frozen tumors by Low Density Array analysis. Results: Large-scale flow cytometry analysis revealed an increased infiltration of T and B but not NK cells in tumors with a high density of Ti-BALT as compared to tumors with a low density. A significant increase proportion of activated and effector-memory T cell subsets was observed in Ti-BALT high versus low tumors. Whereas most T cells located in Ti-BALT had a naive and early memory T cell phenotype, as observed in canonical secondary lymphoid organs, T cells located outside Ti-BALT were significantly enriched in effector-memory T cell phenotype. Finally, Ki-67+ proliferating T cells were found in close contact with mature dendritic cells in Ti-BALT, suggesting a local priming of T cells in these structures. Conclusion: Together, these data suggest that Ti-BALT represent a privileged area for T cell recruitment and activation in the primary site of the tumor. This mechanism could lead to the establishment and maintenance of a protective anti-tumor immune response directly in the tumor. These findings give news insights about mechanisms of T cell infiltration and activation in tumor microenvironment, suggesting new strategies to enhance the efficacy of cancer immunotherapies. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr LB-498. doi:1538-7445.AM2012-LB-498
ChemInform Abstract: KONFORM.‐ UND MEDIUMEFFEKTE BEI INTRAMOLEKULAREN CARBEN‐RK. (1972)

Abstract Das aus p‐Nitrophenyldiazoacetat und Diäthylamin dargestellte Diazoacetamid (I) liefert bei der photochemischen Zersetzung in p‐Dioxan die beiden intramo‐ j lekularen Einschiebungsprodukte (II) und (III) etwa im Mengenverhältnis 1:1.
Prospective Evaluation of Two Lithium Maintenance Dose Schedules (1984)

A prospective method for recommending lithium carbonate maintenance doses for patients requiring the drug either prophylactically or for acute mania has been previously described. Of 18 patients who required prophylactic lithium levels between 0.40 and 0.89 mEq/liter, the maintenance dose equation achieved the predefined therapeutic range in 15 (83%) cases. Of 20 patients requiring lithium concentrations between 0.90 and 1.30 mEq/liter for the treatment of acute mania, 17 (85%) attained the therapeutic range after receiving the recommended maintenance dose. The authors discuss the factors that limit the usefulness of this prospective dosing protocol.
Comparative Toxicity of p,p'- and o,p'-DDT to Larvae of Anopheles quadrimaculatus (1946)

Journal Article Comparative Toxicity of p,p'- and o,p'-DDT to Larvae of Anopheles quadrimaculatus Get access Howard A. Jones, Howard A. Jones U.S.D.A., Agr. Res. Adm., Bureau of Entomology and Plant Quarantine. Search for other works by this author on: Oxford Academic PubMed Google Scholar Harry H. Incho, Harry H. Incho U.S.D.A., Agr. Res. Adm., Bureau of Entomology and Plant Quarantine. Search for other works by this author on: Oxford Academic PubMed Google Scholar Christian C. Deonier Christian C. Deonier U.S.D.A., Agr. Res. Adm., Bureau of Entomology and Plant Quarantine. Search for other works by this author on: Oxford Academic PubMed Google Scholar Journal of Economic Entomology, Volume 39, Issue 5, 1 October 1946, Pages 672–673, https://doi.org/10.1093/jee/39.5.672 Published: 01 October 1946
Adipose tissue PCB levels and<i>CYP1B1</i>and<i>COMT</i>genotypes in relation to breast cancer risk in postmenopausal Danish women (2013)

Exposure to PCBs may be an etiologic factor for breast cancer. The cytochrome P450 1B1 (CYP1B1) and catechol-O-methyltransferase (COMT) enzymes are involved in estrogen metabolism and PCB metabolism, both of which may relate to breast cancer susceptibility. Polymorphisms in genes regulating these enzymes control efficiency. Our objective was to assess whether CYP1B1 and COMT gene polymorphisms modulate the effect of PCBs in breast cancer risk, among postmenopausal Danish women. Neither CYP1B1 Leu432Val polymorphisms nor adipose tissue PCBs were independently associated with breast cancer risk. When assessing the independent effect of the COMT Val158Met polymorphism, we observed reduced risk for breast cancer amongst hormone replacement therapy using women who were homozygous carriers of the variant allele compared with those carrying the wild-type variant (RR = 0.41; 95% CI: 0.29-0.89). We found no statistically significant interactions between any of the PCB groups and CYP1B1 or COMT polymorphisms on the risk of breast cancer.
Optical Network Simulation and the MONET DC Network (2000)

We have used simulation for setting component requirements, assessing component compatibility, and designing experiments in the MONET (Multiwavelength Optical Networking) Project. This paper gives some examples of the types of simulations that have been performed.
Digital Control System Design (2001)

Central difference: e(k + 1)- e(k-1) 2T Although the central difference method provides the best results since it "averages" over two sample periods, a future error value is needed and so from a programming perspective it is not that useful. The same is true for the forward difference approximation. In general, then, the backward difference is commonly used to approximate the derivative term in our PID algorithm.
Complete solution of 4-level flying capacitor converter for medium-voltage drives with active voltage balancing control with phase-disposition PWM (2014)

This paper presents three-phase four-level FLC based electric motor drive for mining application supplied directly from a 6 kV ac-grid. It describes complete control algorithms of the drive with active voltage balancing control. The proposed control is verified by experiments carried out on down-scale drive prototype of rated power of 35 kVA.
Time-resolved free-electron laser spectroscopy of a copper isoelectronic center in silicon (2005)

Time-resolved ``two color'' photoluminescence is reported from the 944 meV band produced by a low concentration of copper in silicon. Photoluminescence has been generated by a pulsed pump beam, and then partially quenched by a probe beam from a free-electron laser. The kinetics of the quenching allow the absorption cross section for photoionization of the center to be determined without knowing its concentration in the sample. The time-resolved data demonstrate a partial recovery of the luminescence after photoionization, due to repopulation of the ionized traps.
p<i>K</i><sub>a</sub> Shifts in Small Molecules and HIV Protease:  Electrostatics and Conformation (1998)

The generalized Born (GB) approximation is a reasonable electrostatic model that is fast enough for use with extensive conformational sampling. This study combines the GB model with a torsion-space sampling method to compute pKa shifts for a series of dicarboxylic acids and amino acids, and for the active-site aspartyl dyad in HIV-1 protease. The calculations agree rather well with experiment for the small molecules. Conformational analysis shows salt-bridging for the zwitterionic amino acids but otherwise modest electrostatic effects upon mean chain lengths. The calculations also show that through-space electrical fields alone cannot account completely for the observed pKa shifts. The calculations for HIV protease agree reasonably well with experiment, despite the complexity of the system. The present computational approach should be useful for a variety of other applications.
Étude d'une alimentation alternative pour moteur à réluctance variable à double saillance (1998)

Ce memoire presente l'etude d'une alimentation originale alternative pour moteur a reluctance variable a double saillance. Dans le premier chapitre, un rappel des principes de fonctionnement et d'alimentation des moteurs a reluctance variable a double saillance est rappele. Differents types d'alimentations faisant appel a des convertisseurs a commutation douce et des ondes sinusoidales sont presentees. A partir de la constatation que le sens du courant n'est pas impose par principe, un convertisseur rustique est propose. Ce convertisseur direct constitue d'un triac par phase du moteur est utilise pour injecter du courant dans les phases du moteur pendant la croissance de l'inductance. Une commande avec limitation de courant n'est pas necessaire avec ce type d'alimentation. Les resultats obtenus avec cette alimentation sont etudies dans le chapitre 3. Le couple moyen en fonction de la vitesse est explicite, ainsi que l'evolution des pertes dans le moteur. Deux modes de fonctionnement en fonction du rapport de la frequence du reseau a la frequence electrique du moteur sont distingues. Enfin, le courant absorbe par le moteur est etudie et une methode de compensation partielle du facteur de puissance est proposee. Dans le chapitre 4, une amelioration permettant de reduire l'ondulation du courant absorbe est etudiee.