gebhart/patrepeval huggingface dataset:
Multiple luteinizing hormone receptor (LHR) protein variants, interspecies reactivity of anti-LHR mAb clone 3B5, subcellular localization of LHR in human placenta, pelvic floor and brain, and possible role for LHR in the development of abnormal pregnancy, pelvic floor disorders and Alzheimer's disease (2003)

Distinct luteinizing hormone receptor (LHR) protein variants exist due to the posttranslational modifications. Besides ovaries, LHR immunoreactivity (LHRI) was also found in other tissues, such as the brain, fallopian tube, endometrium, trophoblast and resident tissue macrophages. The 3B5 mouse monoclonal antibody was raised against purified rat LHR. In rat, porcine and human ovaries, the 3B5 identified six distinct LHR bands migrating at approximately 92, 80, 68, 59, 52 and 48 kDa. Characteristic LHRI was detected in rat, human and porcine corpora lutea. During cellular differentiation, subcellular LHR distribution changed from none to granular cytoplasmic, perinuclear, surface, nuclear and no staining. There were also differences in vascular LHR expression--lack of LHRI in ovarian vessels and strong staining of vessels in other tissues investigated. In normal human term placentae, villous LHRI was associated with blood sinusoids and cytotrophoblast cells, and rarely detected in trophoblastic syncytium. In all abnormal placentae, the LHRI of sinusoids was absent, and syncytium showed either enhanced (immature placental phenotypes) or no LHRI (aged placental phenotype). LHRI in human brain was identified in microglial cells (CD68+ resident macrophages). Protein extracts from human vaginal wall and levator ani muscle and fascia showed strong approximately 92 and 68 kDa species, and LHRI was detected in smooth muscle cells, fibroblasts, resident macrophages and nuclei of skeletal muscle fibers. Our observations indicate that, in contrast to the theory on the role of vascular hormone receptors in preferential pick up of circulating hormones, there is no need to enhance selective pick up rather only prevent LH/CG transport to inappropriate sites. Abnormal placental LHR expression may play a role in the development of abnormal pregnancy. Expression of LHR in the pelvic floor compartments suggests that high LH levels in postmenopausal women may contribute to the pelvic floor relaxation and increased incidence of pelvic floor disorders. Since chorionic gonadotropin increases secretion of a variety of cytokines by monocytes, and induces their inflammatory reaction and phagocytic activity, high LH levels in aging individuals may also activate microglia (mononuclear phagocyte system in the central nervous system) and contribute to the development of Alzheimer's disease and other inflammation-mediated neurodegenerative diseases.
Atmospheric-pressure diffuse coplanar surface discharge for surface treatments (2002)

We report results from a plasma source; a diffuse coplanar surface discharge (DCSD), which is capable of generating macroscopically uniform thin layers of diffuse plasmas in air and other reactive gases at atmospheric pressure. DCSD is a type of dielectric barrier discharge generated on the surface of a dielectric barrier with embedded electrodes, which appears to be advantageous to surface treatment and deposition processes. Preliminary results on hydrophilization of polypropylene nonwoven fabric are also presented.
Coherent Plasmon-Polariton-Phonon Emission from Ultra-High Mobility Carriers (2008)

Interaction between streaming carrier plasmons and lattice vibrations in ultra-high mobility materials leads to unstable excitation of novel, hybrid plasmon-polariton-phonon modes. Spontaneous growth from noise and THz lasing from nano-stuctures is investigated.
Cardiovascular and pharmacological implications of haem-deficient NO-unresponsive soluble guanylate cyclase knock-in mice (2015)

Oxidative stress, a central mediator of cardiovascular disease, results in loss of the prosthetic haem group of soluble guanylate cyclase (sGC), preventing its activation by nitric oxide (NO). Here we introduce Apo-sGC mice expressing haem-free sGC. Apo-sGC mice are viable and develop hypertension. The haemodynamic effects of NO are abolished, but those of the sGC activator cinaciguat are enhanced in apo-sGC mice, suggesting that the effects of NO on smooth muscle relaxation, blood pressure regulation and inhibition of platelet aggregation require sGC activation by NO. Tumour necrosis factor (TNF)-induced hypotension and mortality are preserved in apo-sGC mice, indicating that pathways other than sGC signalling mediate the cardiovascular collapse in shock. Apo-sGC mice allow for differentiation between sGC-dependent and -independent NO effects and between haem-dependent and -independent sGC effects. Apo-sGC mice represent a unique experimental platform to study the in vivo consequences of sGC oxidation and the therapeutic potential of sGC activators.
Cow's milk consumption, HLA-DQB1 genotype, and type 1 diabetes: a nested case-control study of siblings of children with diabetes. Childhood diabetes in Finland study group. (2000)

The evidence for the putative role of cow's milk in the development of type 1 diabetes is controversial. We studied infant feeding patterns and childhood diet by structured questionnaire (n = 725) and HLA-DQB1 genotype by a polymerase chain reaction-based method (n = 556) in siblings of affected children and followed them for clinical type 1 diabetes. In a nested case-control design in a population who had both dietary and genetic data available, we selected as cases those siblings who progressed to clinical diabetes during the follow-up period (n = 33). For each case, we chose as matched control subjects siblings who fulfilled the following criteria: same sex, age within 1 year, not from the same family, the start of the follow-up within 6 months of that of the respective case, and being at risk for type 1 diabetes at the time the case presented with that disease (n = 254). The median follow-up time was 9.7 years (range 0.2-11.3). Early age at introduction of cow's milk supplements was not significantly associated with progression to clinical type 1 diabetes (relative risk adjusted for matching factors, maternal education, maternal and child's ages, childhood milk consumption, and genetic susceptibility markers was 1.60 [95% CI 0.5-5.1]). The estimated relative risk of childhood milk consumption for progression to type 1 diabetes was 5.37 (1.6-18.4) when adjusted for the matching and aforementioned sociodemographic factors, age at introduction of supplementary milk feeding, as well as for genetic susceptibility markers. In conclusion, our results provide support for the hypothesis that high consumption of cow's milk during childhood can be diabetogenic in siblings of children with type 1 diabetes. However, further studies are needed to assess the possible interaction between genetic disease susceptibility and dietary exposures in the development of this disease.
First Observation Of The Wavelength-Dependent Photoproduction Of The 4E,15Z Configurational Isomer Of Bilirubin Bound To Human Serum Albumin (1987)

The photochemistry of bilirubin (BR) is of considerable interest because of its importance in the treatment of neonatal jaundice with visible light phototherapy. Patients are irradiated with blue, white or green fluorescent lamps to induce conversion of the unexcretable and toxic bilirubin to more polar, water-soluble and easily excreted photoproducts. The molecular mechanisms responsible for the phototherapeutic action of light on jaundiced babies have not jet been completely elucidated.
Automated Electrophysiology Assays (2004)

Technological breakthroughs in electrophysiological techniques, such as development of voltage-clamp and patch clamp, have enabled direct measurements of membrane currents in single cells. The low throughput of traditional electrophysiology, however has limited its use in the drug discovery process. High-throughput, non-electrophysiology based primary assays were used successfully but lack key information content, such as voltage dependence and kinetics, and may not be suitable for all ion channels. Most importantly, non-electrophysiological assays lack the ability to accurately control membrane potential, preventing the selective targeting of disease relevant conformational states of voltage-gated channels. For these reasons, substantial efforts were devoted to the development of instruments capable of automated electrophysiological recording at moderate- to high-throughput.Early on, a number of different approaches were investigated (1). The field quickly settled on planar substrates, and in 2002 two automated electrophysiology instruments came on the market: PatchXpress ® and IonWorks ™ HT developed at Axon Instruments and Essen BioSciences, respectively, and later sold to Molecular Devices. Several other manufacturers launched their automated instruments in subsequent years; most notably Sophion (QPatch), Nanion (Patchliner®) and Fluxion (IonFlux). Based on learnings obtained with first generation devices, a new set of second generation instruments was developed in more recent years.Instruments differ substantially in the composition of the patch plate, the configuration of the electrodes, liquid handling methods, and the software used to control the experiment and analyze data, and these features are discussed in greater detail below. Each platform has its strengths and weaknesses, and consideration should be given to the intended purpose before a device is purchased.
Bone marrow time-activity curves of [68Ga]-tilmanocept exhibit molar dose-dependency (2013)

1421 Objectives To determine if Ga-68-labeled tilmanocept (DTPA-mannosyl-dextran), which has a sub-nanomolar affinity for CD206 (a receptor on macrophage surfaces), exhibits molar dose-dependent uptake by bone marrow on dynamic and whole body PET. Kinetic sensitivity to molar dose implies receptor-mediated uptake. Methods Eight pigs (11.5 - 16.5 kg) were positioned for dynamic imaging of the pelvis and proximal tibias on a PET/CT. A low (0.045 umol, n=4) or a high (1.5 umol, n=4) molar dose of Ga-68-labeled tilmanocept (0.73 - 1.2 mCi) was injected into to an ear vein and dynamic imaging (15 min, 0.5 min/fr) was immediately started followed by a 5-bed whole body scan (3 min/bed). Time-SUV curves of the proximal tibia were used to calculate the time required to reach 90% of the peak SUV value (T90). The maximum SUVs (SUVmax) for various tissues were calculated from the whole body scan. Results Radiolabeling was complete within 15 min with a radiolabeling yield in excess of 98% and stable for at least 2 hrs. The dynamic images showed, the mean T90 of 1.52 ± 0.19 min for the high molar group, which was significantly (P=0.0096) higher than the T90 for the low dose group (1.10 ± 0.13 min). The whole body image showed mean SUVmaxes of 1.43 ± 0.31, 1.47 ± 0.26, 1.36 ± 0.44, 0.15 ± 0.10, 17.9 ± 2.1 and 0.62 ± 0.32 for the tibia, femur, lumbar vertebrae, muscle, liver and lung, respectively the low molar group, For the high molar group, the mean SUVmaxs were higher at 2.32 ± 0.80, 2.04 ± 0.62, 1.77 ± 0.74, 0.30 ± 0.14, 12.4 ± 4.1 and 1.01 ± 0.37 for tibia, femur, lumbar vertebrae, muscle, liver, and lung, respectively, but not statistically significant different (P=0.1352-0.3949). Conclusions Ga-68-labeled tilmanocept exhibited molar dose-dependent pharmacokinetics in bone marrow and is more apparent using the T90 from the kinetic data as compared to SUV measurements on static imaging.
Disability management and the Disability Discrimination Act (1997)

This article examines the implications that the Disability Discrimination Act is likely to have for existing organisational practices relating to the treatment of employees who acquire a disability during the course of their employment. Drawing on claims experience under the Americans with Disabilities Act, the authors conclude that if employers are to minimise their legal difficulties they need to adopt a proactive approach to disability management, encompassing both preventive and remedial strategies. They further suggest that the HR function is the best place to locate responsibility for ensuring that such an approach is adopted and effectively implemented.
VERATRUM ALKALOIDS. XXXIV. THE CONFIGURATION OF PROTOVERINE<sup>1</sup> (1959)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTVERATRUM ALKALOIDS. XXXIV. THE CONFIGURATION OF PROTOVERINE1S. Morris Kupchan, Tadashi Masamune, and C. Ian AyresCite this: J. Am. Chem. Soc. 1959, 81, 17, 4753Publication Date (Print):September 1, 1959Publication History Published online1 May 2002Published inissue 1 September 1959https://pubs.acs.org/doi/10.1021/ja01526a084https://doi.org/10.1021/ja01526a084research-articleACS PublicationsRequest reuse permissionsArticle Views38Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Identification of a virB10 protein aggregate in the inner membrane of Agrobacterium tumefaciens (1990)

Products of the virB operon are proposed components of a membrane-associated T-DNA transport apparatus in Agrobacterium tumefaciens. Here we identified the virB10 gene product and raised specific antiserum to the protein. While the virB10 reading frame contains two potential ATG translation start sites located 32 codons apart, we found that only the downstream ATG was required for efficient VirB10 synthesis. Cellular localization studies and analysis of translational fusions with the Escherichia coli alkaline phosphatase gene (phoA) indicated that VirB10 was anchored in the inner membrane and contained a periplasmic domain. This work also demonstrated the utility of alkaline phosphatase as a reporter for secreted proteins in A. tumefaciens. Several high-molecular-weight forms of VirB10 were observed after treatment of A. tumefaciens whole cells or inner membranes with protein cross-linking agents, suggesting that VirB10 exists as a native oligomer or forms an aggregate with other membrane proteins. These results provide the first biochemical evidence that a VirB protein complex is membrane associated in A. tumefaciens.
Flux Recovery from Primal Hybrid Finite Element Methods (2002)

A flux recovery technique is introduced and analyzed for the computed solution of the primal hybrid finite element method for second-order elliptic problems. The recovery is carried out over a single element at a time while ensuring the continuity of the flux across the interelement edges and the validity of the discrete conservation law at the element level. Our construction is general enough to cover all degrees of polynomials and grids of triangular or quadrilateral type. We illustrate the principle using the Raviart--Thomas spaces, but other well-known related function spaces such as the Brezzi--Douglas--Marini (BDM) or Brezzi--Douglas--Fortin--Marini (BDFM) space can be used as well. An extension of the technique to the nonlinear case is given. Numerical results are presented to confirm the theoretical results.
Reliability aspects of the low-frequency noise behaviour of submicron CMOS technologies (1999)

This overview concentrates on reliability aspects of low-frequency (LF) noise in present-day CMOS technologies and transistors. It focuses on how different degradation mechanisms affect the LF noise behaviour and discusses the impact of technological modifications intended to enhance the lifetime of components and circuits. Whenever possible, the physical background will be highlighted, although for a detailed treatment dedicated reviews have to be consulted. The paper consists of two main parts. One covers the degradation mechanisms on a transistor level, including the impact of homogeneous and hot-carrier degradation and the effect of back-end processing steps and, more specifically, plasma damage on the LF noise. In the second part, the noise aspects of the metal interconnect lines related to the occurrence of electromigration are summarized. Because of the downscaling of technologies this aspect gains more and more weight and is, at the same time, a vital part of modern CMOS technology. Throughout the presentation, the possibilities of using noise as a lifetime-predictive tool are discussed and illustrated.
Apoptotic killing of B-chronic lymphocytic leukemia tumor cells by allicin generated <i>in situ</i> using a rituximab-alliinase conjugate (2005)

Allicin, a highly active component from freshly crushed garlic, is produced upon the reaction of the small molecular weight molecule alliin, with the enzyme alliinase (EC 4.4.1.4). Because allicin was shown to be toxic to various mammalian cells in vitro, we devised a novel approach for the therapy of B-cell malignancies based on site-directed generation of allicin. Alliinase was conjugated to the monoclonal antibody rituximab, which recognizes the CD20 antigen, and the resulting conjugate was targeted to CD20+ B chronic lymphocytic leukemia (B-CLL) and other B-cell lymphomas. Upon addition of alliin, allicin was formed in situ, killing the CD20+ tumor B cells via apoptosis. Following a 72-hour treatment, an 85% and 96% reduction was observed in the number of viable B-CLL and EBV-transformed B cells, respectively. Using the human/mouse radiation chimera for the evaluation of allicin targeting in a preclinical animal model, we showed a significant reduction in the number of recovered B-CLL, mantle cell lymphoma, or EBV-transformed B cells. We conclude that our system offers a new powerful and less toxic therapy for B-CLL and other B-cell malignancies. Furthermore, combining alliinase with the appropriate monoclonal antibody may extend the application of this approach to other conditions in which the elimination of a specific cell population is desired.
Thermohydrologic behavior at an underground nuclear waste repository (2002)

We present a multiscale model that simulates coupled thermal and hydrological behavior driven by radioactive decay heat from a potential nuclear waste repository at Yucca Mountain, Nevada. We use this model to evaluate repository performance for different designs with respect to major thermal design goals (e.g., keeping waste packages dry). A locally boiling or globally boiling design uses rock dry out to create dry (low relative humidity (RH)) conditions around waste packages for a long period of time. A subboiling design eliminates boiling in the host rock (possibly reducing uncertainty) but is less effective at maintaining dry conditions. The addition of backfill increases the duration of RH reduction significantly without added boiling in the host rock but at the cost of higher waste package temperatures. The interaction between engineering design variables and natural system factors that affect thermohydrologic behavior is highly nonlinear. As a consequence, designs that differ by seemingly small details can result in markedly different thermohydrologic behavior and consequently repository performance, whereas with regards to other details, large differences may have little or no effect.
Fetal and neonatal outcome of exposure to anticoagulants during pregnancy (1993)

We studied fetal and neonatal outcome of women maintained on anticoagulants (warfarin and/or heparin) during pregnancy. Among 22 Chinese families, 13 mothers (59%) had a history of recurrent abortion or stillbirth while being maintained on warfarin treatment. Twenty-nine liveborn children (17 boys, 12 girls), ages 0.6-11.3 years at follow-up, were analysed for evidence of embryopathy. These were subdivided into 2 groups. Group 1 consisted of 18 children (12 boys, 6 girls) whose mothers were only given warfarin during pregnancy. Five were small for gestational age, and 12 had features of warfarin embryopathy such as nasal hypoplasia. One had subependymal intraventricular hemorrhage shown on neonatal ultrasonography. Group 2 consisted of 11 children (5 boys, 6 girls) whose mothers were maintained on warfarin and heparin during pregnancy. Three were premature deliveries, and 4 had nasal hypoplasia. One had cleft lip, cleft palate, cataract, microphthalmia, intraventricular hemorrhage, and hydrocephalus. We found that despite the high risk of fetal wastage, there was a relative lower risk of major complications, except for some minor cosmetic defects such as nasal hypoplasia. This might lead to readjustment of advice concerning contraception given to pregnant women who were maintained on anticoagulant therapy.
Universal Features of the Fluid to Solid Transition for Attractive Colloidal Particles (2002)

Attractive colloidal particles can exhibit a fluid to solid phase transition if the magnitude of the attractive interaction is sufficiently large, if the volume fraction is sufficiently high, and if the applied stress is sufficiently small. The nature of this fluid to solid transition is similar for many different colloid systems, and for many different forms of interaction. The jamming phase transition captures the common features of these fluid to solid translations, by unifying the behavior as a function of the particle volume fraction, the energy of interparticle attractions, and the applied stress. This paper describes the applicability of the jamming state diagram, and highlights those regions where the fluid to solid transition is still poorly understood. It also presents new data for gelation of colloidal particles with an attractive depletion interaction, providing more insight into the origin of the fluid to solid transition.