gebhart/patrepeval huggingface dataset:
Porphyromonas pasteri sp. nov., isolated from human saliva (2015)

A bacterial strain, designated KUFDS01T, isolated from human saliva was characterized using a polyphasic taxonomic approach that included analysis of physiological and biochemical features, cellular fatty acid profiles and phylogenetic position based on 16S rRNA gene sequence analysis. Cells of the strain were obligately anaerobic, non-pigmented, non-spore-forming, non-motile, Gram-stain-negative rods. Growth of the strain was inhibited on medium containing 20% bile. The 16S rRNA gene sequence analysis showed that the strain was a member of the genus Porphyromonas. Strain KUFDS01T was closely related to Porphyromonas catoniae JCM 13863T (96.6% sequence similarity). An hsp60 gene sequence analysis indicated that strain KUFDS01T was different from P. catoniae JCM 13863T, with a sequence similarity value of 87.8%. The major cellular fatty acids of strain KUFDS01T were C16 : 0, iso-C15 : 0, anteiso-C15 : 0, C18 : 2ω6, 9c and C18 : 1ω9c. The DNA G+C content of strain KUFDS01T was 57.7 ± 0.66 mol%. On the basis of these data, strain KUFDS01T represents a novel species of the genus Porphyromonas, for which the name Porphyromonas pasteri sp. nov. is proposed. The type strain of P. pasteri is KUFDS01T ( = JCM 30531T = CCUG 66735T).
Airborne boundary layer flux measurements of trace species over Canadian boreal forest and northern wetland regions (1994)

Airborne heat, moisture, O 3 , CO, and CH 4 flux measurements were obtained over the Hudson Bay lowlands (HBL) and northern boreal forest regions of Canada during July–August 1990. The airborne flux measurements were an integral part of the NASA/Arctic Boundary Layer Expedition (ABLE) 3B field experiment executed in collaboration with the Canadian Northern Wetlands Study (NOWES). Airborne CH 4 flux measurements were taken over a large portion of the HBL. The surface level flux of CH 4 was obtained from downward extrapolations of multiple‐level CH 4 flux measurements. Methane source strengths ranged from −1 to 31 mg m 2− d −1 , with the higher values occurring in relatively small, isolated areas. Similar measurements of the CH 4 source strength in the boreal forest region of Schefferville, Quebec, ranged from 6 to 27 mg m −2 d −1 and exhibited a diurnal dependence. The CH 4 source strengths found during the ABLE 3B expedition were much lower than the seasonally averaged source strength of 51 mg m −2 d −1 found for the Yukon‐Kuskokwim delta region of Alaska during the previous ABLE 3A study. Large positive CO fluxes (0.31 to 0.53 parts per billion by volume (ppbv) m s −1 ) were observed over the inland, forested regions of the HBL study area, although the mechanism for the generation of these fluxes was not identified. Repetitive measurements along the same ground track at various times of day near the Schefferville site also suggested a diurnal dependence for CO emissions. Measurements of surface resistance to the uptake of O 3 (1.91 to 0.80 s cm −1 ) for the HBL areas investigated were comparable to those observed near the Schefferville site (3.40 to 1.10s cm −1 ). Surface resistance values for the ABLE 3B study area were somewhat less than those observed over the Yukon‐Kuskokwim delta during the previous ABLE 3A study. The budgets for heat, moisture, O 3 , CO, and CH 4 were evaluated. The residuals from these budget studies indicated, for the cases selected, a moderate net photochemical production of O 3 present in the boundary layer over the HBL that coincided with an in situ destruction of CO, although the mechanism responsible for the destruction of CO was not identified. Results from the O 3 budget analysis indicate the importance of in situ photochemical production and its possible dominance over surface deposition to the local O 3 budget at the Schefferville site. Measurements of the in situ production of O 3 indicated a direct relationship between the presence of biomass burning or large‐scale pollution effects. Residuals from budget calculations for conserved quantities (heat, moisture, and CH 4 ) were compared with their respective surface fluxes to provide a measure of the internal selfconsistency of the flux measurements.
Buffeting of a flexible fin over a delta wing (2001)

Buffeting of a flexible fin due to the leading-edge vortex flow over a delta wing was investigated Tip acceleration of the fin as well as unsteady wing surface pressure were measured to understand the interaction of the fin with vortex breakdown. There is a strong asymmetry with regard to the effect of fin location on the buffeting response. For outboard fin locations, the buffeting is small, even at high angles of attack when vortex breakdown is well upstream. For inboard fin locations, the largest fin vibrations were found for breakdown locations well upstream of the fin, and when the shear layer of the breakdown region impinges on the fin. Maximum buffeting response occurs when the fin is located near the vortex axis for low angles of attack, but the location of the maximum buffeting shifts to inboard locations as the angle of attack is increased. The results show that single-fin buffeting may be as important as twin-fin buffeting. The effect of fin flexibility is likely to be negligible when the fin buffeting over aircraft becomes important.
Sequence of the amino-terminal 349 residues of rabbit muscle glycogen phosphorylase including the sites of covalent and allosteric control (1978)

The sequence of the amino-terminal 349 residues of rabbit muscle glycogen phosphorylase (EC 2.4.1.1) has been determined. Limited proteolysis of native phosphorylase b (841 residues, subunit molecular weight 97 412) by subtilisin BPN', Streptomyces alkaline protease, or elastase yielded two large segments (light and heavy). The light segment isolated from the subtilisin digest was cleaved at methionyl bonds with cyanogen bromide to yield eight major fragments and two minor overlapping fragments. The alignment of the major fragments was obtained by analysis of the two minor fragments, of five tryptic peptides containing methionine and of one large fragment generated by cleavage of an aspartylproline bond. Analysis of two cyanogen bromide fragments (CB14 and CB17) isolated from the intact molecule identified the sites susceptible to limited proteolysis and the overlap between the light and the heavy segments. Serine-14 and tyrosine-155 were identified as the residues involved in the covalent and allosteric controls of the enzyme, respectively. Residues 108 and 142 were identified as the cysteine residues reported to be involved in the aggregation of subunits.
Quantitative Determination of the Polar Order Induced under High Electric Field in Amorphous PDR1M Azobenzene Polymer Films (2003)

Thin films of amorphous pDR1M azobenzene homopolymer have been wire poled under high-field conditions. All poled films show a very efficient axial and also a strong polar ordering, as shown by polarized UV−visible measurements and SHG experiments. One of the most striking experimental evidences is an increasing new narrow absorption band around 400 nm, which is detected by using p-polarized light under various incidence angles. Such a spectral feature has already been observed in a few azobenzene-containing liquid-crystal polymers. The poled structures are very stable at room temperature: more than one year after poling, 70% of the initial SHG signal is still preserved in films with a thickness larger than 150 nm. The macroscopic polar ordering is nearly destroyed upon heating the poled film at near 80 °C, i.e., ∼45 °C below the Tg of the starting polymer. A proposed general approach allows the determination of the anisotropic optical constants that simulates nicely all the polarized absorption spectra at several angles of incidence. In particular, the induced birefringence after poling is evaluated as high as ∼0.21 at 1.064 μm, far from any charge-transfer resonance. Finally, from a detailed comparative study of the electronic spectra of a DR1 solution in chloroform and of an unpoled, a poled, and a thermally depoled film, we have shown that the absorption spectra can be nicely deconvoluted into three main bands. Most of the spectroscopic changes upon dc field poling are then qualitatively and quantitatively explained; they come from a significant intensity decrease of the high-energy π−π* transition (−12%) in favor of the low-energy DT band (+12%) and the estimated 〈P2〉 order parameters for these transitions are quite large (∼+0.50), confirming a high degree of ordering.
Pharmacokinetics (PK) of arsenic trioxide in cancer patients (pts) with renal dysfunction: Preliminary results (2004)

2018 Background: Trisenox (arsenic trioxide- ATO) is approved for treatment of relapsed/refractory acute promyelocytic leukemia. This study was designed to determine the PK of ATO in adult cancer pts with renal dysfunction and to evaluate its safety. Methods: 14 pts (median age 66 yrs, 29–85) in 4 renal function (RF) groups (normal- CLCR >80 mL/min; mild dysfunction: 50–80; moderate: 30–49; severe: <30) received IV ATO 0.15 mg/kg/d twice weekly for 4 wks, followed by 2 weeks without drug. Pts were treated for 2 six-week cycles. Arsenious acid (AsIII), arsenic acid (AsV), monomethyl arsonic acid (MMA), and dimethylarsinic acid (DMA) concentrations in plasma and urine were determined by validated methods based on anion exchange HPLC combined with ICPMS. Results: AsIII was rapidly distributed throughout the body (Table). MMA and DMA concentrations reached maximum values 24 hr after ATO. Urinary excretion of all As-containing species accounted for 27% of the dose (days 1–3). No significant correlations were found between AsIII PK parameters and RF, expressed by the CLCR value, except for urinary excretion, which was linearly correlated with CLCR (p<0.01). Exposure to inactive metabolites MMA and DMA inversely correlated with RF (p<0.05). After repeated dosing, plasma concentrations and excretion of methylated metabolites in urine were greater than those observed after the 1st administration, suggesting accumulation. The only severe drug-related toxicity was 1 grade 3 hyperglycemia. Conclusions: Renal excretion does not appear to be the main elimination route for AsIII, which is extensively metabolized to MMA and DMA. Systemic exposure of patients to AsIII, the active species, appears unaffected by RF, even in pts with severe dysfunction. Some accumulation of slowly eliminated metabolites MMA and DMA occurs on repeated dosing. ATO is well-tolerated at this dose in this pt population. Author Disclosure Employment or Leadership Consultant or Advisory Stock Ownership Honoraria Research Funding Expert Testimony Other Remuneration Cell Therapeutics, Inc. Cell Therapeutics, Inc. Cell Therapeutics, Inc. Cell Therapeutics, Inc.
Influence of Macrocyclic Chelators on the Targeting Properties of 68Ga-Labeled Synthetic Affibody Molecules: Comparison with 111In-Labeled Counterparts (2013)

Affibody molecules are a class of small (7 kDa) non-immunoglobulin scaffold-based affinity proteins, which have demonstrated substantial potential as probes for radionuclide molecular imaging. The use of positron emission tomography (PET) would further increase the resolution and quantification accuracy of Affibody-based imaging. The rapid in vivo kinetics of Affibody molecules permit the use of the generator-produced radionuclide (68)Ga (T1/2=67.6 min). Earlier studies have demonstrated that the chemical nature of chelators has a substantial influence on the biodistribution properties of Affibody molecules. To determine an optimal labeling approach, the macrocyclic chelators 1,4,7,10-tetraazacylododecane-1,4,7,10-tetraacetic acid (DOTA), 1,4,7-triazacyclononane-N,N,N-triacetic acid (NOTA) and 1-(1,3-carboxypropyl)-1,4,7- triazacyclononane-4,7-diacetic acid (NODAGA) were conjugated to the N-terminus of the synthetic Affibody molecule ZHER2:S1 targeting HER2. Affibody molecules were labeled with (68)Ga, and their binding specificity and cellular processing were evaluated. The biodistribution of (68)Ga-DOTA-ZHER2:S1, (68)Ga-NOTA-ZHER2:S1 and (68)Ga-NODAGA-ZHER2:S1, as well as that of their (111)In-labeled counterparts, was evaluated in BALB/C nu/nu mice bearing HER2-expressing SKOV3 xenografts. The tumor uptake for (68)Ga-DOTA-ZHER2:S1 (17.9 ± 0.7%IA/g) was significantly higher than for both (68)Ga-NODAGA-ZHER2:S1 (16.13 ± 0.67%IA/g) and (68)Ga-NOTA-ZHER2:S1 (13 ± 3%IA/g) at 2 h after injection. (68)Ga-NODAGA-ZHER2:S1 had the highest tumor-to-blood ratio (60 ± 10) in comparison with both (68)Ga-DOTA-ZHER2:S1 (28 ± 4) and (68)Ga-NOTA-ZHER2:S1 (42 ± 11). The tumor-to-liver ratio was also higher for (68)Ga-NODAGA-ZHER2:S1 (7 ± 2) than the DOTA and NOTA conjugates (5.5 ± 0.6 vs.3.3 ± 0.6). The influence of chelator on the biodistribution and targeting properties was less pronounced for (68)Ga than for (111)In. The results of this study demonstrate that macrocyclic chelators conjugated to the N-terminus have a substantial influence on the biodistribution of HER2-targeting Affibody molecules labeled with (68)Ga.This can be utilized to enhance the imaging contrast of PET imaging using Affibody molecules and improve the sensitivity of molecular imaging. The study demonstrated an appreciable difference of chelator influence for (68)Ga and (111)In.
Stimulation of Somatostatin Release from Median Eminence Tissue Incubated in Vitro by Taurine and Related Amino Acids* (1985)

To determine if the putative amino acid transmitter taurine could affect the release of somatostatin (SRIF) from median eminence fragments, median eminences were incubated in vitro in the presence of various concentrations of taurine and its precursors, hypotaurine, cysteamine, cysteic acid, and L-cysteine sulfinic acid. Taurine was effective in releasing SRIF at doses of 50 microM and higher. Surprisingly, cysteic acid was the most potent stimulant of SRIF release, with a minimal effective dose of 0.1 microM. Cysteamine was effective at a dose of 50 microM, hypotaurine was effective only at 200 microM, and cysteine sulfinic acid was ineffective in stimulating SRIF release at any of the doses tested. To determine if the effect of taurine was mediated by the dopaminergic system, the dopamine receptor blocker pimozide (1 microM) was added to the medium containing either taurine or cysteic acid. Pimozide did not prevent the stimulatory effect of the amino acids. In conclusion, these investigations suggest that taurine and related amino acids may be involved in SRIF release by a nondopaminergic pathway. In view of the high concentrations of taurine in the hypothalamus it must be considered a putative transmitter releasing SRIF.
DNA sequences of polyoma virus early deletion mutants (1981)

The DNA sequences of four "early" viable deletion mutants of polyoma virus have been determined. Two of these (dl-8 and dl-23) are mutants with deletions in the region of the genome that codes for parts of both large and middle T-antigens, and two (dl-6 and dl-28) are mutants with deletions around the viral origin of replication. The former mutants have altered transformation properties relative to wild-type virus, and dl-8 appears to be replication deficient (B. E. Griffin and C. Maddock, J. Virol. 31:645-656, 1979). Sequences are discussed in terms of the altered phenotypes observed for the various mutants, the DNA structures and protein sequences that are affected by the deletions, and how these might affect the biological properties of the mutants.
Structure and electrocatalytic properties of the peroxidase-Nafion composite (2009)

Morphology of nanostructured composite material “horse-radish peroxidase-Nafion” is studied by using atomic-force microscope. The results of the AFM-measurements are compared with the electrochemical data on the hydrogen peroxide reduction in the presence of the horse-radish peroxidase adsorbed from aqueous solution and from the “horse-radish peroxidase-Nafion” composite. The results of the electrochemical measurements and the AFM-data are in qualitative agreement with each other. It is shown that the co-immobilization of the horse-radish peroxidase and Nafion results in the enzyme strong immobilization on the carbonaceous material surface; cluster-structured composites are formed, in which the horse-radish peroxidase demonstrates high catalytic activity.
Differentiation of squamous cell carcinoma and inverted papilloma using non-invasive MR perfusion imaging (2015)

The pseudocontinuous arterial spin labelling technique can be a useful non-invasive diagnostic tool to differentiate SCC from IP in nasal or sinonasal cavity.
Dual Infections of Culex tritaeniorhynchus with West Nile Virus and Nosema algerae (1981)

Culex tritaeniorhynchus females infected with the microsporidian Nosema algerae, and uninfected control females were compared for susceptibility to infection with West Nile (WN) virus and for the ability to transmit virus. When fed on a high titered dose fo virus, 95% of the control females became infected, whereas only 65% of the N. algerae-infected females were infected with WN virus. However, at two lower viral doses, no differences in susceptibility were observed. No significant differences in transmission ability were found between the N. algerae-infected and control females when tested at 10, 14, and 21 days after infection with WN virus. Also, in mosquitoes dually infected with N. algerae and WN virus, neither agent affected the ability of the other to replicate.
Identification of Chlamydia in Cervical Smears by Immunofluorescence: Technic, Sensitivity, and Specificity (1986)

Immunofluorescent staining was compared to cell culture for the diagnosis of Chlamydia trachomatis infections in the female genital tract. By culture, the incidence in the 496 patients in the study was 12.3%. Immunofluorescence demonstrated organisms in 90% of the culture-positive cases, with a specificity of 99.3%. This method appears to be a successful replacement for culture for general screening.
Thermal reactions of some tricyclo[5.3.0.02,10]decatriene derivatives (1970)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThermal reactions of some tricyclo[5.3.0.02,10]decatriene derivativesEdwin Vedejs, Robert A. Shepherd, and Richard P. SteinerCite this: J. Am. Chem. Soc. 1970, 92, 7, 2158–2159Publication Date (Print):April 1, 1970Publication History Published online1 May 2002Published inissue 1 April 1970https://pubs.acs.org/doi/10.1021/ja00710a072https://doi.org/10.1021/ja00710a072research-articleACS PublicationsRequest reuse permissionsArticle Views43Altmetric-Citations9LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Convenient synthesis, separation, and x-ray crystal structure determinations of 1(e),3(e),5(e)-trimethylcycloborazane and 1(e),3(e),5(a)-trimethylcycloborazane (1986)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTConvenient synthesis, separation, and x-ray crystal structure determinations of 1(e),3(e),5(e)-trimethylcycloborazane and 1(e),3(e),5(a)-trimethylcycloborazaneChaitanya K. Narula, Jerzy F. Janik, Eileen N. Duesler, Robert T. Paine, and Riley SchaefferCite this: Inorg. Chem. 1986, 25, 19, 3346–3349Publication Date (Print):September 1, 1986Publication History Published online1 May 2002Published inissue 1 September 1986https://pubs.acs.org/doi/10.1021/ic00239a006https://doi.org/10.1021/ic00239a006research-articleACS PublicationsRequest reuse permissionsArticle Views319Altmetric-Citations33LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Coupled simulation of carrier transport and electrodynamics: the EMC/FDTD/MD technique (2015)

In order to understand the response of conductive materials to high-frequency electrical or optical excitations, the interplay between carrier transport and electrodynamics must be captured. We present our recent work on developing EMC/FDTD/MD, a self-consistent coupled simulation of semiclassical carrier transport, described by ensemble Monte Carlo (EMC), with full-wave electrodynamics, described by the finite-difference time-domain (FDTD) technique and molecular dynamics (MD) for sub-grid-cell interactions. Examples of room-temperature terahertz-frequency transport simulation of doped silicon and back-gated graphene are shown.
Regulatory Roles for Cytokine- Producing B Cells in Infection and Autoimmune Disease (2004)

Recent experiments have revealed that B cells can regulate the course of immune responses to pathogens and autoantigens by antibody-independent mechanisms. One antibody-independent function of B cells is to produce cytokines. In this review we describe the identification of IL-10-producing 'regulatory' B cells as well as IFNgamma-producing 'effector' Bel cells and IL-4-producing 'effector' Be2 cells. We discuss the roles of antigen, pathogen-derived molecules and T cell and dendritic cell-derived factors in regulating the differentiation of mature B cells into cytokine-producing effector B cells. We also review the recent experiments showing that B cell-derived cytokines play pathologic as well as protective roles in immune responses to autoantigens, and demonstrate that cytokine-producing B cells play unexpectedly complex and potentially opposing roles in autoimmune disease.