gebhart/patrepeval huggingface dataset:
Metagenomic exploration of the marine sponge mycale hentscheli uncovers multiple polyketide-producing bacterial symbionts (2020)

© 2020 Storey et al. Marine sponges have been a prolific source of unique bioactive compounds that are presumed to act as a deterrent to predation. Many of these compounds have potential therapeutic applications; however, the lack of efficient and sustainable synthetic routes frequently limits clinical development. Here, we describe a metag-enomic investigation of Mycale hentscheli, a chemically gifted marine sponge that pos-sesses multiple distinct chemotypes. We applied shotgun metagenomic sequencing, hybrid assembly of short-and long-read data, and metagenomic binning to obtain a comprehensive picture of the microbiome of five specimens, spanning three chemo-types. Our data revealed multiple producing species, each having relatively modest secondary metabolomes, that contribute collectively to the chemical arsenal of the holo-biont. We assembled complete genomes for multiple new genera, including two species that produce the cytotoxic polyketides pateamine and mycalamide, as well as a third high-abundance symbiont harboring a proteusin-type biosynthetic pathway that appears to encode a new polytheonamide-like compound. We also identified an additional 188 biosynthetic gene clusters, including a pathway for biosynthesis of peloruside. These re-sults suggest that multiple species cooperatively contribute to defensive symbiosis in M. hentscheli and reveal that the taxonomic diversity of secondary-metabolite-producing sponge symbionts is larger and richer than previously recognized. IMPORTANCE Mycale hentscheli is a marine sponge that is rich in bioactive small mol-ecules. Here, we use direct metagenomic sequencing to elucidate highly complete and contiguous genomes for the major symbiotic bacteria of this sponge. We identify complete biosynthetic pathways for the three potent cytotoxic polyketides which have previously been isolated from M. hentscheli. Remarkably, and in contrast to previous studies of marine sponges, we attribute each of these metabolites to a different producing mi-crobe. We also find that the microbiome of M. hentscheli is stably maintained among in-dividuals, even over long periods of time. Collectively, our data suggest a cooperative mode of defensive symbiosis in which multiple symbiotic bacterial species cooperatively contribute to the defensive chemical arsenal of the holobiont.
Diffuse-charge dynamics in electrochemical systems (2004)

The response of a model microelectrochemical system to a time-dependent applied voltage is analyzed. The article begins with a fresh historical review including electrochemistry, colloidal science, and microfluidics. The model problem consists of a symmetric binary electrolyte between parallel-plate blocking electrodes, which suddenly apply a voltage. Compact Stern layers on the electrodes are also taken into account. The Nernst-Planck-Poisson equations are first linearized and solved by Laplace transforms for small voltages, and numerical solutions are obtained for large voltages. The "weakly nonlinear" limit of thin double layers is then analyzed by matched asymptotic expansions in the small parameter epsilon= lambdaD/L, where lambdaD is the screening length and L the electrode separation. At leading order, the system initially behaves like an RC circuit with a response time of lambdaDL/D (not lambdaD2/D), where D is the ionic diffusivity, but nonlinearity violates this common picture and introduces multiple time scales. The charging process slows down, and neutral-salt adsorption by the diffuse part of the double layer couples to bulk diffusion at the time scale, L2/D. In the "strongly nonlinear" regime (controlled by a dimensionless parameter resembling the Dukhin number), this effect produces bulk concentration gradients, and, at very large voltages, transient space charge. The article concludes with an overview of more general situations involving surface conduction, multicomponent electrolytes, and Faradaic processes.
Hereditary angioedema due to C1 inhibitor In Belarus: epidemiology, access to diagnosis and seven novel mutations in SERPING1 gene (2020)

Hereditary angioedema due to C1 inhibitor deficiency (C1-INH-HAE) is a rare disease. Few states in developing countries have an adequate management of HAE, but none of them belongs to the former USSR area. This study analyses data from C1-INH-HAE patients from Belarus. Data about clinical characteristics, genetics, access to diagnosis and treatment were collected from 2010 by the Belarusian Research Center for Pediatric Oncology, Hematology and Immunology in Minsk. A questionnaire about attacks, prophylactic (LTP) and on-demand therapy (ODT) was administered to patients. We identified 64 C1-INH-HAE patients belonging to 26 families, 27 (42.2%) of which were diagnosed in the last 3 years. The estimated minimal prevalence was 1:148000. Median age at diagnosis was 29 years, with diagnostic delay of 19 years. Thirty-eight patients answered a questionnaire about therapy. Twenty-seven patients underwent ODT: 9 (33.3%) with appropriate treatments, and 18 (66.7%) with inappropriate treatments. Nine patients used LTP with attenuated androgens and eleven recovered spontaneously. Thirty-two patients answered a questionnaire about attacks and triggers: 368 angioedema attacks were reported, with an average of 10 attacks per year. We found 24 different SERPING1 variants: 9 missenses, 6 in splice sites, 6 small deletions, 2 nonsense, 1 large deletion; 7 have not been previously described. De novo variants were found in 11 patients. C1-INH-HAE diagnosis and management in Belarus is improved as seen from the high number of new diagnosis in the last 3 years. Next steps will be to reduce the diagnostic delay and to promote the LTP and OTD.
Cationic Polymerization of Cyclic Dienes. IX. The Structure of Poly(1,3-cyclohexadiene) and Poly(cis, cis-1,3-cycloöctadiene) (1969)

Abstract The structure and properties of poly(1, 3-cyclohexadiene) and poly(cis, cie-1, 3-cycloöctadiene) obtained in the cationic polymerization were investigated.
A Compact High-Performance Patch Antenna Array for 60-GHz Applications (2015)

In this letter, a compact and high-performance patch antenna array is developed for 60-GHz applications. The method to enhance both the gain and the frequency bandwidth of the antenna array simultaneously by using a parallel-series feed network together with the differential feeding technique is studied thoroughly. The tested antenna array achieves operation bandwidth ( ) from 55 to 68 GHz, which covers worldwide 60-GHz unlicensed band of 57-66 GHz, with peak gain of 12.8-15.6 dBi. The measured -3-dB beamwidths at 61.5 GHz are around ±13 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">°</sup> in E-plane and ±15 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">°</sup> in H-plane, respectively. The designed high-gain antenna array has a compact size of only 20 ×14 mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> excluding the connector footprint for test.
Steady-State Pharmacokinetics of Rufloxacin in Elderly Patients with Lower Respiratory Tract Infections (1992)

The pharmacokinetics of rufloxacin, after repeated doses, was evaluated in 12 elderly patients with lower respiratory tract infections. Patients were given a single loading dose of 400 mg on the first day of treatment and single daily maintenance doses of 200 mg for the next 6–9 days. Serum concentrations of the drug were determined by high-performance liquid chromatography (HPLC) at regular intervals during treatment and fitted to a one-compartment open model for repeated doses. The maximum serum concentration after the first dose was 6.46 ± 1.06 (mean ± SEM) μ/ml and was reached in 4.3 ± 0.8 h after the first administration. The elimination half-life was 28.7 ± 4.1 h. The area under the serum levels-time curve from 0 to 24 h was 103 ± 14 μg/h/ml after the first dose. On the last day of observation it increased to 155 ± 28 μg/h/ml, with a mean extent of accumulation of 2.3 ± 0.3 times. The elimination half-life was comparable to those in other studies in healthy young subjects, while plasma levels were about 80% higher. These results suggest that in elderly patients elevated drug concentrations may be reached in the serum. Although no untoward reactions related either to the drug concentration in serum or the dose have been noted with rufloxacin, this patient population should nevertheless be monitored carefully for adverse effects.
Transmission of spiking-rate information through layered networks: the role of recurrent and feedback connections (2007)

Background It is a widely accepted tenant that one of the means by which the nervous system and brain encodes information about external stimuli is via the spiking-rate of neurons. Such a spiking-rate code necessitates the transmission of an elevated spiking-rate through successive neurons in a structured network, such as occurs in the auditory and visual pathways. Recent studies, however, have raised serious questions about our understanding of the propagation of such spiking-rate information through structured networks. In networks consisting of successive layers of integrate-and-fire neurons with conductance synapses with feed-forward of information from one layer to the next, it has been found that the mean spiking-rate in deep layers is essentially independent of the input spiking-rate [1]. The neurons within each layer tend to synchronize with each other, resulting in a synchronous volley of action potentials through successive layers, reminiscent of synfire chains. The average spiking-rate in deeper layers either decays to zero or reaches a stable fixed-point, depending upon the model parameters. This behaviour was also observed in an in vitro study using a dynamic patch clamp [2].
The peculiar processing of glucagon and glucagon-related peptides in patients after pancreatectomy. (2002)

The paradoxical rise of IRGs based on the findings of gel filtration chromatography were possibly due to the generated peculiar glicentin-like peptide and pancreatic glucagon from the glucagon precursor, preproglucagon, after pancreatectomy, which is processed in association with the glucose tolerance deficiencies after pancreatectomy.
Herbivore-Induced Defense Response in a Model Legume. Two-Spotted Spider Mites Induce Emission of (<i>E</i>)-<i>β</i>-Ocimene and Transcript Accumulation of (<i>E</i>)-<i>β</i>-Ocimene Synthase in <i>Lotus japonicus</i>  (2004)

Indirect defense of plants against herbivores often involves the induced emission of volatile infochemicals including terpenoids that attract natural enemies of the herbivores. We report the isolation and characterization of a terpene synthase cDNA (LjEbetaOS) from a model legume, Lotus japonicus. Recombinant LjEbetaOS enzyme produced (E)-beta-ocimene (98%) and its Z-isomer (2%). Transcripts of LjEbetaOS were induced in L. japonicus plants infested with two-spotted spider mites (Tetranychus urticae), coinciding with increasing emissions of (E)-beta-ocimene as well as other volatiles, (Z)-3-hexenyl acetate and (E)-4,8-dimethyl-1,3,7-nonatriene, by the infested plants. We suggest that LjEbetaOS is involved in the herbivore-induced indirect defense response of spider mite-infested L. japonicus via de novo formation and emission (E)-beta-ocimene. Mechanical wounding of the leaves or application of alamethicin (ALA), a potent fungal elicitor of plant volatile emission, also induced transiently increased levels of LjEbetaOS transcripts in L. japonicus. However, wounding or ALA did not result in elevated release of (E)-beta-ocimene. Differences in volatile emissions after herbivory, mechanical wounding, or treatment with ALA suggest that neither a single mechanical wounding event nor ALA simulate the effect of herbivore activity and indicate that herbivore-induced emission of (E)-beta-ocimene in L. japonicus involves control mechanisms in addition to up-regulation of LjEbetaOS transcripts.
Phase III, 24‐month study investigating the efficacy and safety of tafluprost vs latanoprost in patients with open‐angle glaucoma or ocular hypertension (2008)

Abstract Purpose This double‐masked, active‐controlled, parallel‐group, multinational, multicentre, phase III, 24‐month study, conducted in 49 centres across 8 countries, investigated the efficacy and safety of tafluprost 0.0015% vs latanoprost 0.005% (once‐daily at 20:00) in 533 patients with open‐angle glaucoma or ocular hypertension. Methods The primary endpoints were change from baseline IOP after 24 months (1.5 mmHg non‐inferiority limit [tafluprost‐latanoprost]) and safety. Results Both treatments yielded a substantial IOP reduction, that was sustained for 24 months (tafluprost ‐7.1 mmHg; latanoprost ‐7.7 mmHg; n=402). Non‐inferiority was shown with ANOVA and almost reached with ANCOVA (upper limits of the 95% CI for the treatment difference were 1.38 and 1.52, respectively; RM models). Most ocular adverse events were mild and comparable between groups (48% vs 44%). Conclusion This 24‐month phase III study demonstrated that both tafluprost and latanoprost yielded a substantial reduction in IOP that was sustained for 24 months, and a similar safety profile. Commercial interest
Chirped-mirror-dispersion-compensated all-solid-state femtosecond Cr:LiSAF laser (2002)

Mode-locked Cr:LiSAF lasers are attractive femtosecond-pulse sources because Cr:LiSAF has a broad emission band (FWHM-200 nm) almost comparable with that of Ti:sapphire and can be directly pumped by diode lasers. Mode-locked Cr:LiSAF lasers could be compact and inexpensive sub-10-fs pulse sources. Recently, based on a precise measurement of Cr:LiSAF dispersion, 10 fs pulses were produced from an all-solid-state femtosecond Cr:LiSAF laser with a prism pair. In this report, we succeed to expand the spectrum of an all-solid-state femtosecond Cr:LiSAF laser using broadband chirped mirrors to produce sub-10-fs pulses.
Amélioration de la prédiction de l'interaction rotor/stator dans un moteur d'avion (2003)

National audience
Left Ventricular Function and Volume with Coronary CT Angiography Improves Risk Stratification and Identification of Patients at Risk for Incident Mortality: Results from 7758 Patients in the Prospective Multinational CONFIRM Observational Cohort Study (2014)

LV dysfunction and volumes measured with cardiac CT angiography augment risk prediction and discrimination for future mortality.
Adult human heart microvascular endothelial cells are permissive for non-lytic infection by human cytomegalovirus (2001)

Our data provide new insights into the mechanism of CMV dissemination to the heart, signalling the need for further investigation of the pathogenetic role of this virus in cardiac disorders.
Sensor-based path planning and motion control for a robotic system for roadway crack sealing (2000)

Discusses a path planning and control method developed for robotic application in roadway crack sealing. Application of autonomous robotics to this operation requires a path planning system that can take data representing roadway cracks from an external sensor, extract connected crack paths, and link paths together to form the Cartesian motion for the robotic system used to seal the crack. Since the operation is performed in an online fashion, the path planning system has to perform kinematic computations based on dead-reckoning data to update the crack profile as the crack sealing vehicle is moving. Stringent requirements are placed on the controller by the errors inherent in the global path generation, along with the fact that to avoid kinematic singularities, the manipulator used is kinematically redundant. The system developed uses a robust controller known as the simplified Cartesian computed torque (SCCT) to guide the end-effector, along with a compliance loop to move the desired trajectory over the crack, while also addressing kinematic redundancy through application of the weighted generalized inverse Jacobian. The contributions of the paper are in developing practical applications of image processing techniques to extract path locations and determine the appropriate Cartesian end-effector paths, as well as the development of a simple but robust compliant redundant robot controller, all for use in the unstructured environment encountered in highway maintenance operations. The system described has been implemented as part of a prototype automated crack sealing machine. Sample results for actual highway data are included.
Echo Contrast Agents Improve Flow Display of Color Doppler: In Vitro Studies (1991)

Flow detection by color Doppler is impaired by low velocity of flow and increasing attenuation and depth of ultrasound penetration. The effects of increased echogenicity on flow detection (Toshiba SSH 65A) were thus studied in a flow model, which yielded similar strengths of Doppler signals as seen in the clinical routine, by adding microbubble solutions to the blood analog fluid (45% aqueous glycerin) and comparing signal strength (score 0-5) prior to and after contrast. The flow within the plexiglass tube with less than 3 degrees angle of coincidence for Doppler interrogation was laminar with a parabolic velocity profile at physiological velocities and pressures. In comparison with various contrast agents at a flow velocity of 18 cm/sec and 19 dB attenuation, flow was not detectable in control color Doppler, after 4 mL of the blood analog fluid, and after 4 mL of an agitated saline solution 9 mg/mL, but visible after 4 mL of Echovist, a polysaccharide solution with reproducible bubble size and concentration and after 4 mL of an agitated polygelatin solution. Increasing concentration of Echovist (50-400 mg/mL) improved flow detection. Echovist 200 mg/mL enhanced the score by 1.1 + -0.6 for velocities 5-20 cm/sec (P less than 0.01), by 1.6 + -0.8 for 40-100 cm/sec (P less than 0.001), and by 1.1 + -0.6 for velocities greater than 150 cm/sec (P less than 0.001).(ABSTRACT TRUNCATED AT 250 WORDS)
Evolution of the nuclear and magnetic structures of TlFe<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" display="inline"><mml:msub><mml:mrow/><mml:mrow><mml:mn>1.6</mml:mn></mml:mrow></mml:msub></mml:math>Se<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" display="inline"><mml:msub><mml:mrow/><mml:mn>2</mml:mn></mml:msub></mml:math>with temperature (2012)

The evolution of the nuclear and magnetic structures of TlFe${}_{1.6}$Se${}_{2}$ was determined in the temperature range 5--450 K using single-crystal neutron diffraction. The Fe layers in these materials develop a corrugation in the magnetically ordered state. A canting away from the block checkerboard magnetic structure is observed in the narrow temperature range between approximately 100 and 150 K. In this same temperature range, an increase in the corrugation of the Fe layers is observed. At lower temperatures, the block checkerboard magnetic structure is recovered with a suppressed magnetic moment and abrupt changes in the lattice parameters. Microstructure analysis at 300 K using atomic-resolution Z-contrast scanning transmission electron microscopy reveals regions with ordered and disordered Fe vacancies, and the iron content is found to be uniform across the crystal. These findings highlight the differences between the alkali-metal and thallium materials, and indicate competition between magnetic ground states and a strong coupling of magnetism to the lattice in TlFe${}_{1.6}$Se${}_{2}$.
A sub 1V self clocked switched capacitor bandgap reference with a current consumption of 180nA (2012)

This paper presents a bandgap reference capable of operating at supply voltages below 1V. In contrast to the vast majority of bandgap circuits the multiplication of the proportional to absolute temperature voltage is done by a switched capacitor network. Thus a reduced chip area is achieved. As a clock is needed for the switching, a relaxation oscillator is deeply integrated into the circuit. Combined the circuits provide a continuous sub bandgap voltage reference output of 615mV and a clock signal with a frequency of roughly 53kHz. The overall current consumption is 180nA at room temperature. The circuit is developed in a 130nm CMOS technology and occupies an active area of 0.0132mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> .
N° 144. — Conductivité électrique du séléniure de cuivre (1970)

Le contrôle électrochimique de l’indice stœchiométrique du séléniure cuivreux autorise la mesure de la conductivité électronique de ce composé à 400 °C, en fonction de la composition. On calcule la variation du libre parcours moyen des trous électroniques avec l’indice stoechiométrique. L’influence de la température sur la conductivité électrique est étudiée pour du séléniure cuivreux mis en présence de cuivre métallique, puis de sélénium.
SiC/SiC Composites: The Effect of Fiber Type and Fiber Architecture on Mechanical Properties (2008)

Woven SiC/SiC composites represent a broad family of composites with a broad range of properties which are of interest for many energy-based and aero-based applications. Two important features of SiC/SiC composites which one must consider are the reinforcing fibers themselves and the fiber-architecture they are formed into. The range of choices for these two features can result in a wide range of elastic, mechanical, thermal, and electrical properties. In this presentation, it will be demonstrated how the effect of fiber-type and fiber architecture effects the important property of "matrix cracking stress" for slurry-cast melt-infiltrated SiC matrix composites, which is often considered to be a critical design parameter for this system of composites.
Studies on Hemagglutination by Rubella Virus. (1967)

SummaryThe hemagglutinin (HA) of rubella virus can be released from serum inhibitor by binding divalent cations with EDTA. The HA is associated with the viral particle. HA can also be obtained by Tween-ether treatment of infected tissue culture fluids, in which case it is released from the intact whole virus. The attachment between red cells and HA is dependent on divalent cations, and removal of the latter from the environment of agglutinated red cells results in elution of HA.
Voice-excited predictive coder (VEPC) implementation on a high-performance signal processor (1985)

In this paper, we discuss the implementation of a medium-bit-rate linear prediction baseband coder on an IBM bipolar signal processor prototype having a high processing capacity. We show that the implementation of our algorithm requires a processing load of 5 MIPS, with a program size of 5K instructions. We then discuss the application of our coder in a normal telephone environment, which requires mu-law to linear PCM conversion and other signal processing functions such as voice activity detection, automatic gain control, echo control, and error recovery. Quality evaluation tests are also reported which show that this type of coder, operating at 7.2 kbps, allows the transmission of telephone speech with communications quality. Moreover, obtained intelligibility scores and speaker recognition levels are high enough to demonstrate that this coder is a good candidate for telephony applications such as digital trunk transmissions, satellite speech communications, secure voice communications, and audio distribution systems.