gebhart/patrepeval huggingface dataset:
Ki-67 reactivity in primary fallopian tube cancers. (2000)

Forty-four cases of primary cancer of the fallopian tube (PFTC) were analyzed as to Ki-67 expression, grade, stage and the cancer histological type. Among patients with an average age of 57.5 years (range 38-70 years), 27 patients were FIGO I, 7 were FIGO II and 10 were FIGO III. Histological classification of PFTC revealed 18 cases of endometrioid type, 9 serous, 7 undifferentiated, 6 urothelial, 2 clear-cell and 2 of other type. Histological grading revealed 11 cases of G1, 16 of G2 and 17 of G3 tumors. The quantity of Ki-67 positive cells was counted on 300 cancer cells in random high-power fields (10 x 40) and recorded as the labeling index (LI, %). Positive staining for Ki-67 was shown in the nuclei in all cases. Ki-67 LI values ranged from 14.2 to 97.2% (median 36.1). Ki-67 LI values were graded as > or = 36.1% as high and <36.1% as low. We did not find any significant differences in Ki-67 LI values among tumors of various clinical stages, histological grades and histological types. The p value was statistically significant only for stage as a prognostic factor.
Preservation and Reproduction of Human Motion Based on a Motion-Copying System (2012)

In this chapter, a novel method for preserving and reproducing human motion based on haptic technology is described. Haptic technology makes it possible to preserve and reproduce human motion using a paired master and slave system. Because it is possible to preserve motion information based on position trajectory and force input, future human support technology that will facilitate skill acquisition, physical rehabilitation will be developed and will facilitate personal adaptation, tele-communication, et cetera. Once human motions are preserved, it will be possible to process them for various applications. For example, being able to reproduce the speed and trajectory of motion will allow for adjustments that fit the desired function. As a result, the temporal and spatial coupling of perception and action can be attained. This type of physical extension technology based on haptics will be important for the future of human support in society.
Oxidation of imidazolepentacyanoiron(II) by hydrogen peroxide (1977)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTOxidation of imidazolepentacyanoiron(II) by hydrogen peroxideMark L. Bowers, Dan Kovacs, and Rex E. ShepherdCite this: J. Am. Chem. Soc. 1977, 99, 20, 6555–6561Publication Date (Print):September 1, 1977Publication History Published online1 May 2002Published inissue 1 September 1977https://pubs.acs.org/doi/10.1021/ja00462a015https://doi.org/10.1021/ja00462a015research-articleACS PublicationsRequest reuse permissionsArticle Views93Altmetric-Citations8LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
De novo transcriptome assembly and analysis of differentially expressed genes of two barley genotypes reveal root-zone-specific responses to salt exposure (2016)

Plant roots are the first organs sensing and responding to salinity stress, manifested differentially between different root types, and also at the individual tissue and cellular level. High genetic diversity and the current lack of an assembled map-based sequence of the barley genome severely limit barley research potential. We used over 580 and 600 million paired-end reads, respectively, to create two de novo assemblies of a barley landrace (Sahara) and a malting cultivar (Clipper) with known contrasting responses to salinity. Generalized linear models were used to statistically access spatial, treatment-related, and genotype-specific responses. This revealed a spatial gene expression gradient along the barley root, with more differentially expressed transcripts detected between different root zones than between treatments. The root transcriptome also showed a gradual transition from transcripts related to sugar-mediated signaling at the root meristematic zone to those involved in cell wall metabolism in the elongation zone, and defense response-related pathways toward the maturation zone, with significant differences between the two genotypes. The availability of these additional transcriptome reference sets will serve as a valuable resource to the cereal research community, and may identify valuable traits to assist in breeding programmes.
Suppression of Reaginic Antibody Formation (1975)

Abstract By the use of the adoptive transfer system in syngeneic mice it was demonstrated that: i) spleen cells of animals tolerized by the i.v. injection of DNP8-MγG 1 month before adoptive transfer into x-irradiated recipients remained unresponsive when further challenged with the sensitizing antigen (DNP2.3-OA administered i.p. with Al (OH)3); by contrast, a significant response (typical of a secondary response) was obtained in recipients of cells from mice which had received only the sensitized antigen; ii) the anti-hapten IgE secondary response of primed cells was not affected by the presence of cells of tolerized mice when these two types of cells were administered and challenged together in x-irradiated recipients; iii) the transfer of spleen cells or serum of mice, tolerized 20 days before harvesting, into intact syngeneic recipients did not interfere with the normal development of the anti-DNP IgE response of the latter; iv) the anti-hapten responses of tolerized animals receiving spleen cells from normal or primed mice together with the sensitizing antigen resulted, respectively, in a primary or secondary response; (v) the unresponsive state of tolerized cells, achieved by immunosuppression of the original donors with respect to the haptenic determinant, was maintained even after two serial exposures of the cells to the immunizing antigen in two consecutive adoptive cell transfers into x-irradiated recipients. These findings provide a strong basis for the interpretation that the hapten-specific tolerance induced in mice by treatment with DNP8-MγG involved the elimination or inactivation of hapten-specific IgE-producing cells or the blockade of the receptors of these cells.
Immunogenicity and pathogenicity of temperature‐sensitive modified respiratory syncytial virus in adult volunteers (1988)

Single temperature-sensitive (ts) mutants of a subgroup A strain of respiratory syncytial (RS) virus whose multiplication is restricted at 39 degrees C in MRC-6 cells and double ts mutants that are restricted at 38 degrees C, were obtained following mutagenesis using 5-fluorouracil and acridine-like compounds. Isolation and propagation of the parental RSS-2 strain of RS virus and its derived ts mutants were carried out entirely in MRC-5 human diploid cells. The immunogenicity and disease-producing ability of four of these mutants and the parental unmodified strain have been assessed by intranasal administration into groups of about 20 adult volunteers. The results of these trials indicate that the capacity of the parental RSS-2 strain to produce upper respiratory tract infection in adults was not diminished by limited propagation in MRC-5 cells. The mutants on the other hand were impaired in this respect to varying extents. The double mutant tslB in particular has characteristics that suggest that it may be suitable for further development as a live RS virus vaccine. It retained near normal immunogenicity and replicative ability in the upper respiratory tract, while exhibiting greatly reduced disease-producing potential.
Polylactones, 7. The mechanism of cationic polymerization of β‐propiolactone and ϵ‐caprolactone (1986)

Abstract Homopolymerisations of β‐propiolactone and ϵ‐caprolactone, initiated by means of methyl trifluorosulfonate, triethyloxonium tetrafluoroborate or acetylium perchlorate, were investigated. Both 1 H and 13 C NMR spectra proved that the alkylating initiators yield polyesters with alkyl ester end groups, indicating a chain growth via alkyl‐oxygen cleavage of the lactone. At temperatures below 100°C cationic polymerizations initiated by alkylating reagents were found to proceed via end groups which may cause degradation due to back‐biting. When ϵ‐caprolactone was reacted with excess methyl triflate, high concentration of triflate ester end groups were formed, whereas in the case of β‐propiolactone active end groups were not detectable by 1 H NMR spectroscopy. Initiation with acetylium perchlorate yielded a polyester with acetate end groups. Acetate end groups were also obtained, when “living” polymers, initiated with methyl triflate, were reacted with acetic anhydride. It could be shown that the formation of acetate end groups does not indicate an electrophilic attack at the endocyclic oxygen. Furthermore, it is discussed that any experimental evidence for a cationic chain growth via acyloxygen bond cleavage is lacking.
Eine Ritter‐Reaktion: direkte elektrophile Trifluormethylierung von Stickstoffzentren mit hypervalenten Iodreagentien (2010)

Durch säurekatalysierte elektrophile Trifluormethylierung von Nitrilen in Gegenwart von Azolen lassen sich N-(Trifluormethyl)iminderivate herstellen. Die Quelle der Trifluormethylgruppe ist ein leicht zugängliches, einfach zu handhabendes, hypervalentes Iod(III)-Reagens (siehe Schema; HNTf2=Bis(trifluormethansulfonyl)imid). Diese Ritter-Reaktion ist eine direkte Methode zur Synthese sonst schwer erhältlicher N-(Trifluormethyl)imidoylazole.
Inhibition of Angiogenesis by Type I Interferons in Models of Kaposi'S Sarcoma (1999)

Kaposi's Sarcoma (KS) is a pathology which occurs with increased frequency and in a particularly aggressive form in AIDS patients. The HIV-1 Tat protein appears to be an important co-factor in the induction of the extensive neo-vascularization associated with AIDS-KS. Tat acts as a chemoattractant for endothelial cells in vitro, inducing both chemotactic and invasive responses. Several clinical trials have been performed testing the effectiveness of diverse biological agents in therapy of KS, among these the type I interferons. Type I IFNs have diverse biological functions besides their anti-viral activity, including anti-angiogenic properties. We have shown that IFN alpha and IFN beta are potent inhibitors of both primary and immortalized endothelial cell migration and morphogenesis in vitro as well as neo-angiogenesis induced by HIV-1 Tat in vivo. The inhibitory effect of IFN class I on HIV-Tat associated angiogenesis further supports its use as a therapy for epidemic Kaposi's sarcoma. The use of recombinant IFNs at the levels required to obtain a therapeutic effect are associated with side effects and toxicity, therefore we are now developing a gene therapy approach for constant and local delivery type I IFNs.
Minimum-Latency FEC Design With Delayed Feedback: Mathematical Modeling and Efficient Algorithms (2020)

In this paper, we consider the packet-level forward error correction (FEC) code design, without feedback or with delayed feedback, for achieving the minimum end-to-end latency, i.e., the latency between the time that packet is generated at the source and its in-order delivery to the application layer of the destination. We first show that the minimum-latency FEC design problem can be modeled as a partially observable Markov decision process (POMDP), and hence the optimal code construction can be obtained by solving the corresponding POMDP. However, solving the POMDP optimally is in general difficult unless its state and action space is very small. To this end, we propose an efficient heuristic algorithm, namely the majority vote policy, for obtaining a high quality approximate solution. We also derive the tight lower and upper bounds of the optimal state values of this POMDP, based on which a more sophisticated D-step search algorithm can be implemented for obtaining near-optimal solutions. The simulation results show that the proposed code designs via solving the POMDP, either with the majority vote policy or the D-step search algorithm, strictly outperform the existing schemes, for both cases, without or with only delayed feedback.
Molecular Mimicry between <i>Helicobacter pylori</i> Antigens and H+,K+–Adenosine Triphosphatase in Human Gastric Autoimmunity (2003)

Autoimmune gastritis and Helicobacter pylori-associated gastric atrophy develop through similar mechanisms involving the proton pump H+,K+-adenosine triphosphatase as autoantigen. Here, we report that H. pylori-infected patients with gastric autoimmunity harbor in vivo-activated gastric CD4+ T cells that recognize both H+, K+-adenosine triphosphatase and H. pylori antigens. We characterized the submolecular specificity of such gastric T cells and identified cross-reactive epitopes from nine H. pylori proteins. Cross-reactive H. pylori peptides induced T cell proliferation and expression of T helper type 1 functions. We suggest that in genetically susceptible individuals, H. pylori infection can activate cross-reactive gastric T cells leading to gastric autoimmunity via molecular mimicry.
ChemInform Abstract: REACTIONS OF PYRAZOLO(1,5‐A)PYRIMIDINE DERIVATIVES WITH NUCLEOPHILES. IV. SOME REACTIONS OF 1,4‐DIHYDROCYCLOPENT(B)INDOLES (1985)

Abstract Das durch Methylierung von (Ia) entstehende Derivat (Ib) wird mit Indol (II) zu den im Titel genannten Verbindungen (IIIi) umgesetzt.
Crystallization and structural analysis of bullfrog red cell L‐subunit ferritins (1994)

Ferritin is a 24 subunit protein that controls biomineralization of iron in animals, bacteria, and plants. Rates of mineralization vary among members of the ferritin family, particularly between L and H type subunits of animal ferritins which are differentially expressed in various cell types. To examine ferritin from a highly differentiated cell type and to clarify the relationship between ferritin structure and function, bullfrog red cell L ferritin has been cloned, overexpressed in E. coli, and crystallized under two conditions. Crystals were obtained at high ionic strength in the presence of MnCl2 at a concentration comparable to that of the protein and in the presence of MgCl2 at a concentration much higher than that of the protein. Under both crystallization conditions, the crystals are tetragonal bipyramids in the space group F432 with unit cell dimensions a = b = c = 182 +/- 0.5 A. Crystals obtained in the presence of manganese and ammonium sulfate diffract to 1.9 A, while those obtained in the presence of magnesium and sodium tartrate diffract to 1.6 A. Isomorphous crystals have been obtained under similar conditions for a site-directed mutant with a reduced mineralization rate in which Glu-57, -58, -59, and -61 are all replaced by Ala. The structure of wild type L-subunit with magnesium has been solved by molecular replacement using the calcium salt of human liver H subunit (Lawson et al., Nature (London) 349:541-544, 1991) as the model. The crystallographic R factor for the 6-2.2 A shell is 0.21. The overall fold of human H and bullfrog L ferritins is similar with an rms difference in backbone atomic positions of 0.97 A. The largest structural differences occur in the D helix and the loop connecting the D and E helices of the four helix bundle. Because red cell L ferritin and liver H ferritin show differences in both rates of mineralization and three-dimensional structure, more detailed comparisons of these structures are likely to shed new light on the relationship between conformation and function.
RADIATIVE TRANSFER THROUGH AN IN HOMOGENEOUS FLY-ASH CLOUD: EFFECTS OF TEMPERATURE AND WAVELENGTH DEPENDENT OPTICAL PROPERTIES (1992)

Abstract The effect of spectral radiative properties of fly-ash particles on radiative heat transfer through an inhomogeneous planar layer near the walls of a combustion system is investigated. The spectral absorption and scattering coefficients, as well as the scattering phase function of the particles, are obtained from the Lorenz-Mie theory as functions of temperature and particle diameter. These data are employed in the solution of the radiative transfer equation, which is modeled using a double spherical harmonics approximation. In the calculations, a linearly anisotropic scattering phase function is used. It is shown that, if the medium temperature distribution is nonuniform, the divergence of the radiative flux term calculated using the spectral fly-ash properties differs significantly from that obtained using constant properties. This means that if fly-ash properties are assumed wavelength independent, a coupled radiation-convection heat transfer analysis would underestimate the gas temperature near the walls of a typical furnace. On the other hand, the temperature dependency of the fly-ash radiative properties is quite weak and can safely be neglected.
Deferred vector map visualization (2016)

Interactive rendering of large scale vector maps is a key challenge for high-quality geographic visualization software systems. In this paper we present a novel approach for the visualization of large scale vector maps over detailed height-field terrains. Our method uses a deferred line shading approach to render large scale vector maps directly in a screen-space shading stage over a terrain visualization. The fact that there is no traditional geometric polygonal rendering involved allows our algorithm to outperform conventional vector map rendering algorithms for geographic information systems. Our flexible clustered deferred line rendering approach allows a user to interactively customize and apply advanced vector styling methods, as well as the integration into a vector map level-of-detail system.
Cardiac Response to Nitric Oxide Synthase Inhibition Using Aminoguanidine in a Rat Model of Endotoxemia (2002)

This study evaluates the effect of aminoguanidine, a preferential inhibitor of inducible nitric oxide synthase (iNOS), on the prevention of cardiac depression in acute endotoxemia. Cardiac performance was evaluated after 4 h of exposure to endotoxin. Rats (n = 5) were selected randomly to receive, by intraperitoneal injection, one of four treatments: saline, LPS (lipopolysaccharide, E. coli, 4 mg/kg, AG (aminoguanidine 100 mg/kg), and LPS + AG at various times. AG and saline treatments were administered 30 min before LPS and at 1 and 3 h after LPS injection. Hearts were perfused using the Langendorff isolated perfusion system and a balloon-tipped catheter was placed into the left ventricle to measure left ventricular developed pressure (LVDP). Myocyte contractile function was assessed with electrical field stimulation and video microscopy. Tissue was immunostained for the expression of iNOS and for nitrotyrosine, a byproduct of protein nitration by peroxynitrite. Perfused hearts from LPS-treated rats exhibited a 57% decrease (P < 0.05) in LVDP compared to saline-treated animals. No improvement in ventricular function was observed with the administration of AG. Similarly, cardiac myocytes prepared from LPS-treated animals demonstrated a significant (P < 0.05) reduction in percent and velocity of shortening and this effect was unaltered with the same dose of AG. AG administration significantly reduced serum nitrite/nitrate levels (P < 0.05) in endotoxemic rats to control levels. Localized expression of iNOS in the myocardium was lessened with AG treatment and was not associated with peroxynitrite formation in this model of endotoxemia. The results indicate that AG given in vivo before and after endotoxin (at a concentration sufficient to decrease NO production) did not reduce cardiac depression. We conclude that selective inhibition of iNOS and the reduction of NO production do not prevent cardiac dysfunction at an early stage in an acute model of endotoxemia.
T cell expression of MyD88 is required for resistance to<i>Toxoplasma gondii</i> (2008)

Resistance to Toxoplasma gondii depends on dendritic cells to recognize this pathogen and secrete IL-12, in turn promoting IFN-gamma production from responding T cells. The adaptor protein, myeloid differentiation primary-response gene 88 (MyD88), is important for most Toll-like receptor (TLR) signaling, as well as IL-1R/IL-18R signals. There is considerable evidence that MyD88 is required for the innate sensing of T. gondii and IL-12 responses. Although Myd88(-/-) mice challenged with T. gondii have defective IL-12 and Th1 effector responses and succumb to disease, administration of IL-12 to Myd88(-/-) mice partially restores the Th1 response and yet fails to prolong survival. This finding suggested that MyD88 may mediate signals within T cells important for resistance to this pathogen. To evaluate the role of MyD88 in T cells under noncompetitive conditions, bone marrow chimeras were generated, in which the T cells lacked MyD88, but MyD88-dependent innate immune responses were intact. Upon challenge with T. gondii, these chimeric mice were more susceptible to disease, developing severe toxoplasmic encephalitis and succumbing within 30 days. Splenocytes and brain mononuclear cells isolated from infected chimeric mice produced less IFN-gamma when cultured with a T. gondii-derived antigen. The increase in susceptibility observed was independent of signals via the IL-1R and IL-18R, suggesting a role for TLRs in MyD88-mediated T cell responses to T. gondii. These observations show that, in addition to a role for MyD88 in innate responses, T cell expression of MyD88 is necessary for prolonged resistance to a pathogen.
First pass vasodilator-stress myocardial perfusion CMR in mice on a whole-body 3Tesla scanner: validation against microspheres (2012)

We report the feasibility of first pass vasodilator-stress myocardial perfusion CMR in mice on a whole-body 3Tesla scanner and demonstrate a 2.6 fold increase of stress over rest myocardial blood flow in good agreement with microspheres.
Temperature dependence of storage time in silicon <i>p</i> <sup>+</sup> - <i>n</i> - <i>n</i> <sup>+</sup> switching diodes and reduction of harmonic distortion (1980)

Using the reverse-recovery technique, storage times in p+-n-n+ diodes have been measured as a function of temperature. In all cases the effective lifetime for injected charge increases with increasing temperature within the interval 0 to 180°C. This result was found to reduce second-harmonic distortion in p+-n-n+ switches by as much as 6 dB at 2.5 MHz.