gebhart/patrepeval huggingface dataset:
Synthesis of Novel Lipids in <i>Saccharomyces cerevisiae</i> by Heterologous Expression of an Unspecific Bacterial Acyltransferase (2004)

The bifunctional wax ester synthase/acyl-coenzyme A:diacylglycerol acyltransferase (WS/DGAT) is the key enzyme in storage lipid accumulation in the gram-negative bacterium Acinetobacter calcoaceticus ADP1, mediating wax ester, and to a lesser extent, triacylglycerol (TAG) biosynthesis. Saccharomyces cerevisiae accumulates TAGs and steryl esters as storage lipids. Four genes encoding a DGAT (Dga1p), a phospholipid:diacylglycerol acyltransferase (Lro1p) and two acyl-coenzyme A:sterol acyltransferases (ASATs) (Are1p and Are2p) are involved in the final esterification steps in TAG and steryl ester biosynthesis in this yeast. In the quadruple mutant strain S. cerevisiae H1246, the disruption of DGA1, LRO1, ARE1, and ARE2 leads to an inability to synthesize storage lipids. Heterologous expression of WS/DGAT from A. calcoaceticus ADP1 in S. cerevisiae H1246 restored TAG but not steryl ester biosynthesis, although high levels of ASAT activity could be demonstrated for WS/DGAT expressed in Escherichia coli XL1-Blue in radiometric in vitro assays with cholesterol and ergosterol as substrates. In addition to TAG synthesis, heterologous expression of WS/DGAT in S. cerevisiae H1246 resulted also in the accumulation of fatty acid ethyl esters as well as fatty acid isoamyl esters. In vitro studies confirmed that WS/DGAT is capable of utilizing a broad range of alcohols as substrates comprising long-chain fatty alcohols like hexadecanol as well as short-chain alcohols like ethanol or isoamyl alcohol. This study demonstrated the highly unspecific acyltransferase activity of WS/DGAT from A. calcoaceticus ADP1, indicating the broad biocatalytic potential of this enzyme for biotechnological production of a large variety of lipids in vivo in prokaryotic as well as eukaryotic expression hosts.
Dealing with constraints: a biomechanical approach (2003)

Quantitative observations are presented on how normal human subjects manipulate a loaded crank. Velocity patterns showed that the task was performed smoothly, despite kinematic singularities which required rapid, precisely timed reversals of joint motion. Significant forces were exerted normal to the crank such that the crank partially supported the weight of the arm. Kinematic data revealed that arm motion depended on the direction of crank motion. These observations suggest that humans use a task-oriented control strategy and may take advantage of motion constraints to reduce required effort.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Turning a Methanation Catalyst into a Methanol Producer: In-Co Catalysts for the Direct Hydrogenation of CO2 to Methanol (2018)

The direct hydrogenation of CO2 to methanol using green hydrogen is regarded as a potential technology to reduce greenhouse gas emissions and the dependence on fossil fuels. For this technology to become feasible, highly selective and productive catalysts that can operate under a wide range of reaction conditions near thermodynamic conversion are required. Here, we demonstrate that indium in close contact with cobalt catalyses the formation of methanol from CO2 with high selectivity (>80%) and productivity (0.86 gCH3OH.gcatalyst-1.h-1) at conversion levels close to thermodynamic equilibrium, even at temperatures as high as 300 °C and at moderate pressures (50 bar). The studied In@Co system, obtained via co- precipitation, undergoes in situ transformation under the reaction conditions to form the active phase. Extensive characterization demonstrates that the active catalyst is composed of a mixed metal carbide (Co3InC0.75), indium oxide (In2O3) and metallic Co.
2D wall profiles in exchange coupled bilayers (1993)

Two-dimensional wall configurations in exchange coupled bilayers are calculated using numerical micromagnetic simulations. Starting with a 180 degrees domain arrangement in zero field, full saturation is, under hard axis field, only reached for field values large with respect to the anisotropy field, due to the nucleation of 360 degrees walls. Their collapse field is computed versus the magnitude of the interlayer exchange coupling. A novel hysteresis mechanism, specifically for large coupling constants and involving superposed 360 degrees walls, is described.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Nitric oxide in the regulation of vasomotor tone in human skeletal muscle (1999)

The role of nitric oxide (NO) as a regulator of vasomotor tone has been investigated in resting and exercising human skeletal muscle. At rest, NO synthase (NOS) inhibition by intra-arterial infusion of NG-monomethyl-L-arginine decreased femoral artery blood flow (FABF, ultrasound Doppler) from 0.39 +/- 0.08 to 0.18 +/- 0.03 l/min (P < 0. 01), i.e., by approximately 52%, and increased leg O2 extraction from 62.1 +/- 9.8 to 100.9 +/- 4.5 ml/l (P < 0.004); thus leg O2 uptake (VO2, 22 +/- 4 ml/min, approximately 0.75 ml. min-1. 100 g-1) was unaltered [not significant (P = NS)]. Mean arterial pressure (MAP) increased by 8 +/- 2 mmHg (P < 0.01). Heart rate (HR, 53 +/- 3 beats/min) was unaltered (P = NS). The NOS inhibition had, however, no effect on the initial rate of rise or the magnitude of FABF (4.8 +/- 0.4 l/min, approximately 163 ml. min-1. 100 g-1), MAP (117 +/- 3 mmHg), HR (98 +/- 5 beats/min), or leg VO2 (704 +/- 55 ml/min, approximately 24 ml. min-1. 100 g-1, P = NS) during submaximal, one-legged, dynamic knee-extensor exercise. Similarly, FABF (7.6 +/- 1.0 l/min, approximately 258 ml. min-1. 100 g-1), MAP (140 +/- 8 mmHg), and leg VO2 (1,173 +/- 139 ml/min, approximately 40 ml. min-1. 100 g-1) were unaffected at termination of peak effort (P = NS). Peak HR (137 +/- 3 beats/min) was, however, lowered by 10% (P < 0.01). During recovery, NOS inhibition reduced FABF by approximately 34% (P < 0.04), which was compensated for by an increase in the leg O2 extraction by approximately 41% (P < 0.04); thus leg VO2 was unaltered (P = NS). In conclusion, these findings indicate that NO is not essential for the initiation or maintenance of active hyperemia in human skeletal muscle but support a role for NO during rest, including recovery from exercise. Moreover, changes in blood flow during rest and recovery caused by NOS inhibition are accompanied by reciprocal changes in O2 extraction, and thus VO2 is maintained.
The effect of poly (ADP-ribose) polymerase inhibition on aminoglycoside-induced acute tubular necrosis in rats (2016)

This study illustrates that PARP inhibitor significantly attenuates gentamicin-induced nephrotoxicity in rats with no effect on the bactericidal activity.
Constrained Design Optimization of Rotor-Tilting Pad Bearing Systems (2010)

Design of a rotor-bearing system is a challenging task due to various conflicting design requirements, which should be fulfilled. This study considers an automatic optimization approach for the design of a rotor supported on tilting-pad bearings. A numerical example of a rotor-bearing system is employed to demonstrate the merits of the proposed design approach. The finite element method is used to model the rotor-bearing system, and the dynamic speed-dependent coefficients of the bearing are calculated using a bulk flow code. A number of geometrical characteristics of the rotor simultaneously with the parameters defining the configuration of tilting pad bearings are considered as design variables into the automatic optimization process. The power loss in bearings, stability criteria, and unbalance responses are defined as a set of objective functions and constraints. The complex design optimization problem is solved using heuristic optimization algorithms, such as genetic, and particle-swarm optimization. Whereas both algorithms found better design solutions than the initial design, the genetic algorithms exhibited the fastest convergence. A statistical approach was used to identify the influence of the design variables on the objective function and constraint measures. The bearing clearances, preloads and lengths showed to have the highest influence on the power loss in the chosen design space. The high performance of the best solution obtained in the optimization design suggests that the proposed approach has good potential for improving design of rotor-bearing systems encountered in industrial applications.
Resistance of W/Fu Rats to Adrenal Regeneration Hypertension (1975)

The procedure for producing adrenal regeneration hypertension did not cause an increase in the systolic blood pressure of W/Fu animals. The regenerating adrenal gland in W/Fu animals was not restored to normal; reduced numbers of mitochondrial cristae were seen and the mitochondria were smaller in size; regeneration was complete in Sprague-Dawley rats of the Holtzman strain and there was a severe form of hypertensive, cardiovascular disease.
Modulation of the Transferred Mouse 26K Casein Gene in Mouse L Cells by Glucocorticoid Hormone1 (1987)

The cloned 26K casein gene was transferred to mouse L-cells and its expression was measured by Northern blot hybridization. When the lambda clone with 5'- and 3'-flanking sequences was transferred, transcripts were detected without glucocorticoid, but in the presence of glucocorticoid, the level of the transcripts of heterogeneous sizes increased and their pattern was similar to that observed in the mammary glands of non-lactating mice. When the 6.7 kb EcoRI fragment containing most of the coding region was transferred, putative precursor and mature mRNAs were detected without glucocorticoid. Surprisingly, with the addition of glucocorticoid, the level of the transcripts greatly decreased. The presence of multiple sequences responsible for glucocorticoid receptor binding was detected in the 5'-flanking region of the gene in a competition assay using the subfragments of casein gene and on sequence analysis. These results suggest that the 26K casein gene has multiple regulatory domains which interact with glucocorticoid receptors, and these domains may play different roles in the regulation of the casein gene.
Searching Chemical Space with the Bayesian Idea Generator (2009)

The Pfizer Global Virtual Library (PGVL) is defined as a set compounds that could be synthesized using validated protocols and monomers. However, it is too large (10(12) compounds) to search by brute-force methods for close analogues of a given input structure. In this paper the Bayesian Idea Generator is described which is based on a novel application of Bayesian statistics to narrow down the search space to a prioritized set of existing library arrays (the default is 16). For each of these libraries the 6 closest neighbors are retrieved from the existing compound file, resulting in a screenable hypothesis of 96 compounds. Using the Bayesian models for library space, the Pfizer file of singleton compounds has been mapped to library space and is optionally searched as well. The method is >99% accurate in retrieving known library provenance from an independent test set. The compounds retrieved strike a balance between similarity and diversity resulting in frequent scaffold hops. Four examples of how the Bayesian Idea Generator has been successfully used in drug discovery are provided. The methodology of the Bayesian Idea Generator can be used for any collection of compounds containing distinct clusters, and an example using compound vendor catalogues has been included.
Refractive Indices of Amorphous and Crystalline HNO3/H2O Films Representative of Polar Stratospheric Clouds (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTRefractive Indices of Amorphous and Crystalline HNO3/H2O Films Representative of Polar Stratospheric CloudsB. S. Berland, D. R. Haynes, K. L. Foster, M. A. Tolbert, S. M. George, and O. B. ToonCite this: J. Phys. Chem. 1994, 98, 16, 4358–4364Publication Date (Print):April 1, 1994Publication History Published online1 May 2002Published inissue 1 April 1994https://pubs.acs.org/doi/10.1021/j100067a024https://doi.org/10.1021/j100067a024research-articleACS PublicationsRequest reuse permissionsArticle Views252Altmetric-Citations43LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Human neutrophil calmodulin-binding proteins: identification of the calmodulin-dependent protein phosphatase (1986)

The molecular events in linking neutrophil activation and ligand binding to specific membrane receptors are mediated in part by an increase in intracellular Ca/sup 2 +/. One mechanism by which Ca/sup 2 +/ may trigger neutrophil activation is through Ca/sup 2 +//calmodulin (CaM)-regulated proteins and enzymes. To determine which Ca/sup 2 +//CaM-regulated enzymes may be present in the neutrophil, they have used Western blotting techniques and /sup 125/I-CaM to identify neutrophil CaM-binding proteins. Eleven proteins with molecular weights ranging from 230K to 13.5K bound /sup 125/I-CaM in a Ca/sup 2 +/-dependent manner. One predominant region of /sup 125/I-Cam binding was to a 59K protein; a protein with an identical mobility was labeled by an antisera against brain CaM-dependent phosphatase. Ca/sup 2 +/-dependent phosphatase activity, which was inhibited by the CaM antagonist trifluoperazine, was detected in a neutrophil extract; a radioimmunoassay for the phosphatase indicated that it was present in the extract at approximately 0.2 ..mu..g/mg protein. Most of the CaM-binding proteins, including the 59K protein, were rapidly degraded upon lysis of the neutrophil. There was a close correlation between the degradation of the 59K protein and the loss of Ca/sup 2 +/-dependent phosphatase activity in the neutrophil extract. Thus, humanmore » neutrophils contain numerous CaM-binding proteins which are presumably Ca/sup 2 +//calmodulin-regulated enzymes and proteins; the 59K protein is a CaM-dependent phosphatase.« less
Distinction between small lymphocytic and mantle cell lymphoma by immunoreactivity for CD23. (1994)

Immunoreactivity for CD23, a B-cell activation antigen, was compared in 22 cases of B-cell small lymphocytic lymphoma (SLL)/chronic lymphocytic leukemia (CLL) and 15 cases of mantle cell lymphoma (MCL) (intermediate lymphocytic lymphoma or centrocytic lymphoma) to determine if the presence of this antigen can be used as a criterion to distinguish between these two low-grade B-cell lymphoproliferative processes. CD23 immunoreactivity was observed in 21 of 22 cases (95%) of SLL/CLL, whereas only 2 of 15 cases (13%) of MCL were reactive for this antigen; therefore, lack of CD23 reactivity is statistically significantly associated with MCL (P < 0.0001). In addition, lack of CD23 immunoreactivity is also statistically significantly associated with SLL with plasmacytoid differentiation in comparison with typical SLL (P < 0.0003), although only a small number of cases were evaluated. SLL/CLL, MCL, and SLL with plasmacytoid differentiation cases were all uniformly immunoreactive for pan-B-cell antigens such as CD20 and CD22. Immunoreactivity for CD23 appears to be a useful marker to aid in distinguishing SLL/CLL from MCL and may be helpful in routine immunophenotypic screening of low-grade B-cell derived lymphoproliferative processes.
Structure of trans-1,4,5,8-tetrathiadecalin (hexahydro-1,4-dithiino-[2,3-b]-1,4-dithiin) (1987)

C 6 H 10 S 4 cristallise dans le systeme monoclinique avec le groupe d'espace P2 1 /n. Affinement de la structure jusqu'a 0,034. Les deux cycles a 6 chainons sont en conformation chaise
The Chlorinium Ion as an Intermediate in Chlorination of Aromatic Compounds by Hypochlorous Acid (1955)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe Chlorinium Ion as an Intermediate in Chlorination of Aromatic Compounds by Hypochlorous AcidC. Gardner Swain and Arthur D. KetleyCite this: J. Am. Chem. Soc. 1955, 77, 12, 3410Publication Date (Print):June 1, 1955Publication History Published online1 May 2002Published inissue 1 June 1955https://pubs.acs.org/doi/10.1021/ja01617a087https://doi.org/10.1021/ja01617a087research-articleACS PublicationsRequest reuse permissionsArticle Views129Altmetric-Citations11LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Technique of management of the left atrioventricular valve in the repair of atrioventricular septal defect with a common atrioventricular orifice (1991)

Summary Despite improvement in the early outcome for patients undergoing complete repair of atrioventricular septal defects with a common atrioventricular orifice, the incidence of reoperation for failure of the repair of the left atrioventricular valve remains high. The surgical techniques employed in our most recent experience, including routine suture of the septal commissure and liberal use of valvar annuloplasty, were successful in achieving a very satisfactory competence of left atrioventricular valve with a iow incidence of reoperation for failure of the repair.
Touch-evoked Agitation Produced by Spinally Administered Phospholipid Emulsion and Liposomes in Rats  (1995)

These results emphasize that certain L-isomeric phospholipids with their gel-transition temperatures near body temperature can produce prominent touch-evoked agitation after spinal delivery, an effect likely mediated by a phospholipase hydrolysis product. This touch-evoked agitation, which is consistent with neurotoxicity reported in the early literature on lysophospholipids, suggests that the choice of lipids for the formulation of liposomes intended for spinal drug delivery should be carefully considered.
168 The Treatment of Erectile Dysfunction in Men post Radical Prostatectomy with Amniotic Fluid injection (2017)

To determine the feasibility of Amniotic Fluid (AF) injection in patients with ED who are status post radical prostatectomy (RALP). Five patients, ranging in age from 49 to 67 years old (mean age 59) at the time of initial injection took part in the study. Baseline International Index of Erectile Function (IIEF-5) scores and penile dopplers to determine peak systolic velocity (PSV) were obtained. Patients who had success with oral ED medications were excluded. Patients were injected with 1 to 2cc of Amniotic Fluid, mixed with 3 to 4 cc of normal saline. The AF used in this study was provided from UroStem. AF is a minimally manipulated, acellular fluid that primarily comes from the genitourinary system and surrounds the fetus. Of the five patients who were treated with AF post RALP, three of the five patients filled out an IIEF-5 score. The IIEF-5 score ranged from 5 to 11 before AF injection, with an average of 7. The post AF IIEF-5 score ranged from 5 to 18, with an average of 9.3. The PSV prior to injection was obtained from all 5 patients and ranged from 8.6cm/s to 38.2cm/s with an average of 18.8. The post-AF PSV was obtained from 4 out of the 5 patients and ranged from 19.0cm/s to 66.0cm/s, averaging 37.9cm/s. Three out of the five patients (75%) noted improvement with ED after injections. One of the two patients that said that he did not notices an improvement with his ED did however note a notable improvement in his curvature and a decrease in his plaque size from 5Lx1Hx 5W mm to 2Lx1Hx2W mm, as well as increase in PSV from 12.3cm/s to 66.0cm/s. The other patient did not return for follow up visits. Four out of the five patients had a positive response within the first injection, and were happy with overall results.
Distributed multiagent learning with a broadcast adaptive subgradient method (2010)

Many applications in multiagent learning are essentially convex optimization problems in which agents have only limited communication and partial information about the function being minimized (examples of such applications include, among others, coordinated source localization, distributed adaptive filtering, control, and coordination). Given this observation, we propose a new non-hierarchical decentralized algorithm for the asymptotic minimization of possibly time-varying convex functions. In our method each agent has knowledge of a time-varying local cost function, and the objective is to minimize asymptotically a global cost function defined by the sum of the local functions. At each iteration of our algorithm, agents improve their estimates of a minimizer of the global function by applying a particular version of the adaptive projected subgradient method to their local functions. Then the agents exchange and mix their improved estimates using a probabilistic model based on recent results in weighted average consensus algorithms. The resulting algorithm is provably optimal and reproduces as particular cases many existing algorithms (such as consensus algorithms and recent methods based on the adaptive projected subgradient method). To illustrate one possible application, we show how our algorithm can be applied to coordinated acoustic source localization in sensor networks.
Modélisation d'un transducteur piézo-céramique ultrasonore vibrant en épaisseur avec VHDL-AMS (2004)

International audience
Assessing the Fidelity of Haptically Rendered Deformable Objects (2006)

A central element of surgical simulators is the generation of appropriate haptic feedback. Several factors influence this rendering process, which could potentially degrade the feedback quality. In this context, our current research aims at assessing, how well haptic sensations encountered during interaction with real objects, can actually be approximated in a virtual environment. Since finding appropriate soft tissue deformation models for real time interaction is a complex task in itself, we limited the investigations in this pilot study to simple linear-elastic silicone objects. A model of a virtual deformable object was adapted and parameters selected to approximate a real silicone sample within a specific hardware setup. A comparative study was performed, in which 13 subjects had to discriminate between the categories of real and virtual objects. We found that subjects could discern between the categories with a mean accuracy of 63% with no significant bias towards assuming the presence of either real or virtual objects. While being above chance level, the results indicate that we were able to approximate haptic feedback of a real object with high fidelity in our specific hardware setup.