gebhart/patrepeval huggingface dataset:
Fringe-free, Background-free, Collinear Third Harmonic Generation FROG Measurements for Multiphoton Microscopy (2006)

Collinear pulse measurement tools useful at the full numerical aperture (NA) of multiphoton microscope objectives are a necessity for a quantitative characterization of the femtosecond pulses focused by these systems. In this letter, we demonstrate a simple new technique, for characterizing the pulse at the focus in a multiphoton microscope. This technique, a background-free, fringe-free, form of frequency-resolved optical gating, uses the third harmonic signal generated from a glass coverslip. Here it is used to characterize 100 fs pulses (typical values for a multiphoton microscope) at the focus of a 0.65 NA objective.
The ZO-1–associated Y-box factor ZONAB regulates epithelial cell proliferation and cell density (2003)

Epithelial tight junctions regulate paracellular permeability, restrict apical/basolateral intramembrane diffusion of lipids, and have been proposed to participate in the control of epithelial cell proliferation and differentiation. Previously, we have identified ZO-1–associated nucleic acid binding proteins (ZONAB), a Y-box transcription factor whose nuclear localization and transcriptional activity is regulated by the tight junction–associated candidate tumor suppressor ZO-1. Now, we found that reduction of ZONAB expression using an antisense approach or by RNA interference strongly reduced proliferation of MDCK cells. Transfection of wild-type or ZONAB-binding fragments of ZO-1 reduced proliferation as well as nuclear ZONAB pools, indicating that promotion of proliferation by ZONAB requires its nuclear accumulation. Overexpression of ZONAB resulted in increased cell density in mature monolayers, and depletion of ZONAB or overexpression of ZO-1 reduced cell density. ZONAB was found to associate with cell division kinase (CDK) 4, and reduction of nuclear ZONAB levels resulted in reduced nuclear CDK4. Thus, our data indicate that tight junctions can regulate epithelial cell proliferation and cell density via a ZONAB/ZO-1–based pathway. Although this regulatory process may also involve regulation of transcription by ZONAB, our data suggest that one mechanism by which ZONAB and ZO-1 influence proliferation is by regulating the nuclear accumulation of CDK4.
Real-valued parallel clonal selection algorithm for design optimization in electromagnetics (2010)

In this paper, we demonstrate that a successful parallel implementation of CLONALG would decrease the computational burden of multiple calls to the electromagnetic solver by distributing the work load over multiple processors. The optimization of a multi-layered frequency selective surface (FSS) filter for the X-band will be presented and compared with the more traditional parallel genetic algorithm (GA) optimizer in order to demonstrate the effectiveness of the parallel CLONALG implementation in the area of electromagnetics design.
Magnetic force microscopy study of track edge effects in longitudinal media (1997)

Magnetic force microscopy (MFM) is used to investigate the micromagnetics at the track edges of recorded bits as a function of linear bit density. Dipolar edge charges are observed in two types of isotropic media but are not detected in an oriented medium. We study track edge percolation and the resulting loss of effective track width as a function of bit density in the range of 10–300 kfci. Analysis of the power spectral density (PSD) of the MFM signal as a function of cross-track location shows that at high bit densities the dominant PSD peak shifts to lower frequencies at the track edges as compared to the track center. This is discussed in terms of track edge percolation that leads to magnetically correlated regions with size larger than the bit length.
mRNA-dependent synthesis of authentic precursor to human placental lactogen: conversion to its mature hormone form in ascites cell-free extracts. (1976)

Messenger RNA derived from term placenta directs the synthesis of human placental lactogen (hPL, molecular weight 22,200) in an ascites 30,000 X g post-mitochondrial supernate (S-30). When the S-30 is fractionated into ribosome and cell-sap (S-100) fractions, and these are recombined for incubation, term placental mRNA directs the synthesis of a protein with a molecular weight of 25,000. This protein contains authentic hPL tryptic peptides. This suggested that during the separation of ribosomes and S-100 a component responsible for cleavage was lost. A 1.0 M sucrose cushion was used for the preparation of ribosomes and S-100 and membranous material accumulated at the sucrose interphase. When this membrane fraction was added back to the ribosome-S-100 system only hPl was formed. Cleavage was greatest when membranes were added within the first few minutes of incubation. In a run-off system composed of term polysomes, ascites S-100, and the inhibitor of initiation, pactamycin, the 25,000 molecular weight material, referred to as pre-hPL, was also synthesized. These data strongly suggest that (i) pre-hPL is an authentic percursor to hPL, (ii) cleavage of the precursor primarily occurs on nascent, ribosome-bound peptide chains, and (iii) pre-hPL is the primary gene product.
Hyperbolic Velocity Model (2013)

Asymptotically bounded velocity profiles describe the vertical velocity variations in compacted sediments in a more realistic way than unbounded velocity models, and allow presenting the subsurface by a smaller number of thicker layers. The first and the simplest asymptotically bounded model is the Hyperbolic velocity profile proposed by Muscatin 1937, and our paper is an extension of this early study. The Hyperbolic model has an advantage over other bounded models: The velocity increases with depth and approaches the limiting value with a more smooth and gradual rate. We derive the time-depth relationships, forward and backward transforms between the instantaneous velocity profile and the effective models (average, RMS and fourth order average velocities), study the trajectories for pre-critical and post-critical curved rays and derive the equations for traveltime, lateral propagation and arc length. We compare the ray paths obtained with the Hyperbolic model and with the other bounded velocity profiles.
Phosphonylation of Biomolecules with Inorganic Diphosphonate. II. Phosphonylation of Phosphate Groups on Nudeoside 5′-Monophosphates, Deoxynudeoside 5′-Monophosphates, and Sugar Phosphates (1988)

Abstract Inorganic diphosphonate phosphonylated predominantly phosphate groups on nucleoside 5′-monophosphates (=NMP’s: AMP, GMP, IMP, CMP, and UMP), deoxynudeoside 5′-monophosphates (=dNMP’s: dAMP and dTMP), and sugar phosphates (=SP’s: GlclP, Glc6P, and Rib5P) in aqueous solutions under mild conditions (50–70 °C, pH 6). The chemical structures of phosphonylated products were discussed on their 31P NMR spectra and high-performance liquid chromatograms. The main phosphonylated products from NMP’s and dNMP’s were analogous in structure to nucleoside 5′-diphosphates. The rate of phosphonylation of phosphate groups on NMP’s, dNMP’s, and SP’s varied strongly depending on reaction conditions: pH, reaction temperature, and initial concentration of inorganic diphosphonate. The maximum yields of phosphonylated products were ca. 70% for all NMP’s, dNMP’s, and SP’s under the following conditions: initial concentration of inorganic diphosphonate is 1.5 mol dm−3; each initial concentration of NMP’s, dNMP’s, or SP’s is 0.1 mol dm−3; 70 °C; pH 6.
Oxidative Amidation of Methylarenes and Heteroamines under Metal‐Free Conditions (2017)

Abstract Synthesis of heteroamides through oxidative amidation of methylarenes with hetero aromatic amines using tert ‐Butyl hydroperoxide (TBHP) as oxidant has been described. The present method represents a significant development for the oxidative amidation of methylarenes to obtain heteroamides which does not require metal catalyst and TBHP act as only oxidant. Methylarenes are easy to handle, stable, commercially available (by‐products in gasoline industry) and used as carbonyl source for amide synthesis. The reaction proceeds through the imine intermediate formation and its subsequent oxidation by TBHP provides the desired amides.
Polarization-Type Interference-Fringe Shifter (1972)

Get PDF Email Share Share with Facebook Tweet This Post on reddit Share with LinkedIn Add to CiteULike Add to Mendeley Add to BibSonomy Get Citation Copy Citation Text Olof Bryngdahl, "Polarization-Type Interference-Fringe Shifter," J. Opt. Soc. Am. 62, 462-464 (1972) Export Citation BibTex Endnote (RIS) HTML Plain Text Citation alert Save article
Characterization of a major histocompatibility complex class I antigen-binding glycoprotein from adenovirus type 35, a type associated with immunocompromised hosts (1987)

Adenovirus type 35 (Ad35) is a group B adenovirus that has been isolated primarily from patients with acquired immunodeficiency syndrome and other immunodeficiency disorders. We have studied the interaction of this unique adenovirus with the immune system by analyzing Ad35 early viral proteins in infected HeLa cells. We have identified a 29,000-Mr Ad35 early glycoprotein, E29, which associates with class I antigens of the major histocompatibility complex (MHC) in the endoplasmic reticulum. Ad35 E29 is analogous to the group C Ad2 early glycoprotein E3-19K (E19), which has been shown to interfere with the expression of class I antigens on the cell surface (H. Burgert and S. Kvist, Cell 41:987-997, 1985). In contrast to the Ad2 glycoprotein, Ad35 E29 was synthesized in much smaller amounts, was more extensively glycosylated, and did not cross-react with polyclonal antibody against the Ad2 protein. As a control, a class I antigen-binding glycoprotein from another group B adenovirus, Ad7, was also characterized and was found to have properties similar to those of Ad35 E29. Therefore, the differences in the glycosylation and quantity of class I antigen-binding glycoproteins between Ad35 and Ad2 are group related. Inhibition of the expression of MHC class I antigens, which are needed for cytotoxic-T-lymphocyte recognition of virus-infected cells, appears to play a vital role in the adenovirus life cycle in vivo. Our data indicate that this function has been conserved despite significant differences in the MHC class I antigen-binding glycoprotein and in the pathogenicity between serotypes.
TNFAIP3/A20 as a potential regulator of NF-κB-mediated resistance to O6-alkylating chemotherapy in glioblastoma multiforme (2006)

4493 Resistance to chemotherapy is a major obstacle to the successful treatment of glioblastoma multiforme. There is increasing appreciation that drug resistance in tumor cells emerges from the orchestrated activity of multiple cellular components in complex signaling networks. We have utilized an integrated model of glioblastoma resistance to O 6 -alkylating agents (including Temozolomide and BCNU) and genomics tools to globally explore molecular factors, cellular pathways, and functional interaction networks perturbed during the selection and evolution of drug resistance in glioblastoma cells. We have identified a transcriptomic signature of 329 genes that predicts a common in vitro and in vivo resistance phenotype to these agents (false discovery rate: 0.005), a proportion of which is recurrently imprinted by gene dosage changes in the resistant glioblastoma genome. This signature was highly enriched for genes with functions in cell death, compromise, and survival. Modularity was a predominant organizational principle of the resistance signature, with functions being carried out by groups of interacting molecules in overlapping networks. Our results highlight a key role of a cytoplasmic signaling cascade that activates nuclear factor κB (NF-κB) in response to these agents. Our data indicate the alteration of various members of the NF-κB canonical pathway. Within this pathway, we have identified the tumor necrosis factor α-induced protein 3 (TNFAIP3) gene as a new factor significantly associated with glioblastoma cell resistance. expression of the corresponding zinc finger protein A20 closely mirrored the expression of the TNFAIP3 transcript, and was immediately related to NF-κB activation status (nuclear translocation and DNA-binding) in the resistant phenotype. Translation of these findings into the clinic showed that a prediction model based on our cell model-derived resistance signature enabled us to sub-classify an independent validation cohort of 31 glioblastoma patients into distinct outcome groups ( p = 0.037). We have also revealed TNFAIP3 as part of an optimized four-gene predictor (TNFAIP3, CD44, FBXO32, SDC1) significantly associated with patient survival in glioblastoma multiforme ( p = 0.022). A Cox proportional-hazards regression analysis of the individual genes in this predictor revealed TNFAIP3 to be significantly associated with outcome when considered as individual continuous variable ( p = 0.028). Our results offer strong evidence for TNFAIP3 as a key regulator of the cytoplasmic signaling to activate NF-κB in the emergence of resistance of glioblastoma cells to O 6 -alkylating chemotherapy. Our observations raise the hope for TNFAIP3 as an amenable target to modulate NF-κB-mediated resistance in these cells, with the ultimate goal of increasing the efficacy of chemotherapy in patients harboring these challenging malignancies.
Triggers for β-Sheet Formation at the Hydrophobic–Hydrophilic Interface: High Concentration, In-Plane Orientational Order, and Metal Ion Complexation (2011)

Amyloid formation plays a causative role in neurodegenerative diseases such as Alzheimer's disease or Parkinson's disease. Soluble peptides form β-sheets that subsequently rearrange into fibrils and deposit as amyloid plaques. Many parameters trigger and influence the onset of the β-sheet formation. Early stages are recently discussed to be cell-toxic. Aiming at understanding various triggers such as interactions with hydrophobic-hydrophilic interfaces and metal ion complexation and their interplay, we investigated a set of model peptides at the air-water interface. We are using a general approach to a variety of diseases such as Alzheimer's disease, Parkinson's disease, and type II diabetes that are connected to amyloid formation. Surface sensitive techniques combined with film balance measurements have been used to assess the conformation of the peptides and their orientation at the air-water interface (IR reflection-absorption spectroscopy). Additionally, the structures of the peptide layers were characterized by grazing incidence X-ray diffraction and X-ray reflectivity. The peptides adsorb to the air-water interface and immediately adopt an α-helical conformation. This helical intermediate transforms into β-sheets upon further triggering. The factors that result in β-sheet formation are dependent on the peptide sequence. In general, the interface has the strongest effect on peptide conformation compared to high concentrations or metal ions. Metal ions are able to prevent aggregation in bulk but not at the interface. At the interface, metal ion complexation has only minor effects on the peptide secondary structure, influencing the in-plane structure that is formed in two dimensions. At the air-water interface, increased concentrations or a parallel arrangement of the α-helical intermediates are the most effective triggers. This study reveals the role of various triggers for β-sheet formation and their complex interplay. Our main finding is that the hydrophobic-hydrophilic interface largely governs the conformation of peptides. Therefore, the present study implies that special care is needed when interpreting data that may be affected by different amounts or types of interfaces during experimentation.
Effects of Temperature and Age on Daily Changes in Pheromone Titer in Laboratory-Reared and Wild Gypsy Moth (Lepidoptera: Lymantriidae) (1992)

Effects of temperature, age, and photoperiod on pheromone titer in the pheromone gland of gypsy moth, Lymantria dispar (L.), were studied with the aid of gas chromatography. In laboratory-reared females, daily rhythmic changes in pheromone content were observed with peaks occurring toward the evening and increasing in quantity with female age. In wild females held in outdoor cages under very hot afternoon conditions, maximum pheromone titer was observed in the early morning and minimum titer in the afternoon. However, pheromone-oriented flight activity of the wild males was lowest in the morning and highest in the afternoon. Temperatures of 33 and 35°C caused decline in pheromone titer to near zero in laboratory-reared and wild females, respectively. Calling behavior was not dependent on the presence of pheromone in the gland; it occurred in newly emerged females before pheromone could be detected and in females held at temperatures that inhibited pheromone production.
Protection Against Acute MPTP‐Induced Dopamine Depletion in Mice by Adenosine A<sub>1</sub> Agonist (1993)

The effects of the adenosine A1 agonist N6-cyclohexyladenosine (CHA) on MPTP-induced dopamine (DA) depletion in the striatum of C57BL/6 mice were studied. Twenty hours after a single injection of MPTP (30 mg/kg, s.c.), the toxin caused 62% depletion of striatal DA. CHA (0.2-3 mg/kg, s.c.), when given together with MPTP, prevented the toxin-induced DA depletion in a dose-dependent manner. This protective action was apparently mediated by the A1 receptors, because this effect was selectively antagonized by pretreating the animals with the A1 antagonist 8-cyclopentyl-1,3-dipropylxanthine (25 mg/kg, i.p.) but not with the A2 antagonist 1,3-dipropyl-7-methylxanthine (25 mg/kg, i.p.). When CHA (3 mg/kg) was injected 5 h after MPTP administration, at which point striatal DA levels were already reduced significantly, a rapid and complete recovery of the striatal DA levels occurred. These neurochemical data suggest that the A1 agonist CHA is potentially useful as a neuroprotective agent against MPTP-induced toxicity.
Expression of polyketide biosynthesis and regulatory genes in heterologous streptomycetes (1991)

There are now several examples showing that hybrid secondary metabolites can be produced as a result of interspecies cloning of antibiotic biosynthesis genes in streptomycetes. This paper reviews examples of hybrid secondary metabolite production, and examines the underlying biochemical and regulatory principles leading to the formation of hybrid anthraquinones by recombinant anthracycline-producing streptomycetes carrying actinorhodin biosynthesis genes. An anthraquinone, aloesaponarin II, was produced by cloning the actI, actIII, actIV, and actVII genes (pANT12) of actinorhodin biosynthesis pathway from Streptomyces coelicolor in anthracycline producing streptomycetes. Streptomyces galilaeus strains 31 133 and 31 671, aclacinomycin and 2-hydroxyaklavinone producers, respectively, formed aloesaponarin II as their major polyketide product when transformed with pANT12. Subcloning experiments indicated that a 2.8-kb XhoI fragment containing only the actI and actVII loci was necessary for aloesaponarin II biosynthesis by S. galilaeus 31 133. When S. galilaeus 31 671 was transformed with the actI, actVII, and actIV genes, however, the recombinant strain produced two novel anthraquinones, desoxyerythrolaccin and 1-O-methyldesoxyerythrolaccin. When S. galilaeus 31 671 was transformed with only the intact actIII gene (pANT45), aklavinone was formed exclusively. These experiments indicate a function for the actIII gene, which is the reduction of the keto group at C-9 from the carboxyl terminus of the assembled polyketide to the corresponding secondary alcohol. The effects of three regulatory loci, dauG, dnrR1, and asaA, on the production of natural and hybrid polyketides were also shown.
Adherence of synovial cells on EDA‐containing fibronectin (1996)

EDA+ FN enhances adherence of SC on the matrix via the Hep2 region of EDA+ FN.
DENSITY SEPARATION OF ZYGOTIC AND SOMATIC EMBRYOS (1990)

Density changes associated with developing zygotic embryos of loblolly pine (Pinus taeda L.) and somatic embryos of celery (Apium graveolens L.) were determined using sucrose gradients. Continuous sucrose gradients were used to evaluate relative density of loblolly pine embryos from July 25, when embryos could be microscopically observed, to November 7, 1988. Embryos density declined during development with a maximum (51% sucrose equivalent or 1.2331 g/ml) at first sampling and then remain relatively constant (10% sucrose equivalent or 1.0306 g/ml) after day 49. Density changes were inversely related to embryo length. Celery somatic embryos, cultured for 6, 8, 10, or 12 days were separated with sucrose solutions varying from 9 to 16% in 1% intervals. Embryos were classified as overmature (expanded cotyledons), mature (torpedo), and immature (globular). The number of low density embryos increased from 6 to 12 days. The highest conversion to normal seedlings after desiccation for 48 hr at 90% relative humidity was obtained with overmature and mature embryos, but some immature somatic embryos also survived. Maximum conversion was obtained from embryos with density equivalent of 12% to 14% sucrose (1.0448 g/ml to 1.0531 g/ml) at days 10 and 12.
An anticorrosive magnesium/carbon nanotube composite (2008)

Here, we report a drastically improved anticorrosive characteristic of magnesium alloy composites with the introduction of multiwalled carbon nanotubes. Highly depressed corrosion of nanotube-filled magnesium composite in salt water is due to the formation of stable oxide films along the grain boundaries of magnesium. Our results indicate that carbon nanotube acted as effective multifunctional filler to improve both mechanical and anticorrosive performances of magnesium alloy.
The synthesis of pyrrolizidines and indolizidines by the intramolecular cycloaddition of azides with electron-rich 1,3-dienes. A synthetic equivalent of a nitrene-diene cycloaddition (1990)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe synthesis of pyrrolizidines and indolizidines by the intramolecular cycloaddition of azides with electron-rich 1,3-dienes. A synthetic equivalent of a nitrene-diene cycloadditionWilliam H. Pearson, Stephen C. Bergmeier, Samir Degan, Ko Chung Lin, Yam Foo Poon, Jeffrey M. Schkeryantz, and John P. WilliamsCite this: J. Org. Chem. 1990, 55, 22, 5719–5738Publication Date (Print):October 1, 1990Publication History Published online1 May 2002Published inissue 1 October 1990https://pubs.acs.org/doi/10.1021/jo00309a016https://doi.org/10.1021/jo00309a016research-articleACS PublicationsRequest reuse permissionsArticle Views794Altmetric-Citations63LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
ChemInform Abstract: Belted Spirocyclic Tetrahydrofurans: A New Class of Preorganized Ionophoric Polyethers. Molecular Structure, Conformation, and Binding to Alkali‐Metal Atoms. (1991)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.