gebhart/patrepeval huggingface dataset:
Functional Properties of Purified Vicilins from Cowpea (<i>Vigna unguiculata</i>) and Pea (<i>Pisum sativum</i>) and Cowpea Protein Isolate (2003)

The major storage globulins (vicilins) of cowpea (Vigna unguiculata) and pea (Pisum sativum) seeds were purified by ammonium sulfate precipitation, and a semipurified cowpea protein isolate (CPI) was prepared by isoelectric precipitation. Some of the functional properties of these proteins, including solubility, foaming, and emulsifying capacities, were investigated and compared. The solubility of purified cowpea vicilin was reduced at pH 5.0, increasing markedly below and above this value. Pea vicilin exhibited poor solubility between pH 5.0 and pH 6.0, and CPI was little soluble in the pH range from 4.0 to 6.0. At neutral pH, the emulsifying activity indexes (EAI) of purified pea vicilin and CPI were 194 and 291 m(2)/g, respectively, which compare quite favorably to EAIs of 110 and 133 m(2)/g for casein and albumin, respectively. Remarkably, purified cowpea vicilin exhibited an EAI of 490 m(2)/g, indicating a very high emulsifying activity. Purified cowpea and pea vicilins exhibited lower foaming capacities and foam stablity indexes (FSI) than CPI. FSI values of 80 and 260 min were obtained for purified pea and cowpea vicilin, respectively, whereas a FSI value of 380 min was obtained for CPI. These results are discussed in terms of the possible utilization of purified vicilins or protein isolates from pea and cowpea in the food processing industry.
Electron paramagnetic resonance study of S3− formed on magnesium oxide (1973)

Elemental sulfur reacts at 400° with partially hydroxylated magnesium oxide to form a stable paramagnetic species which has been identified as the S3− ion. The 33S hyperfine splitting is characteristic of an aba type molecule with a‖ (a)=20.7 G, a‖ (b)=39.6G, a⊥ (a)=3.7 G, and a⊥ (b)=7.5G. The principal g values are g‖=2.004 and g⊥=2.043. The g and hyperfine tensors suggest that at 77°K the molecule is librating in the plane of the three sulfur atoms. The formation of S3− is accompanied by the evolution of H2S and the loss of another sulfur radical which is believed to be a polymeric form of sulfur, Sn. These observations suggest that the reaction Sn+4OH−→ 2H2S+2S3−+SO4−+Sn-6 may occur at the surface.
SR<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" altimg="si1.gif" display="inline" overflow="scroll"><mml:mi>μ</mml:mi></mml:math>CT and MRX analysis of ferrofluid accumulation in bovine arteries: a step further in the understanding of Magnetic Drug Targeting (2010)

Cancer is one of the most deadly and widespread diseases nowadays. Local cancer treatments are gaining strong importance. Magnetic Drug Targeting and magnetic heating treatment are minimal invasive cancer treatments that make use of magnetic nanoparticles as drug carriers in the first case and to induce heat transfer in the second case. For MDT the application of drugs, bound to magnetic nanoparticles, is realized via the tumor supplying vessels while a strong magnetic field gradient influences the flow and directs the nanoparticles to the respective region. In order to understand the mechanisms behind this magnetically controlled drug delivery, an ex-vivo artery model has been established. To study the amount and the distribution of magnetic nanoparticles within the arteries, microcomputed tomography and magnetorelaxometry have been used as analytical techniques. MRX provides very sensitively the amount of magnetic nanoparticles, while microcomputed tomography based on a synchrotron beam line enables 3-dimensional non-destructive examination of the distribution of the magnetic nanoparticles at a spatial resolution of a few micrometers. In this paper we present first results concerning the quantitative study of accumulation of nanoparticles within bovine arteries streamed with magnetic nanoparticles.
ChemInform Abstract: Synthesis of a New Tetracyclic System Related to Aptazapine (CGS 7525A) by One‐Pot Double Anellation. (1989)

Abstract 2‐Pyrrolylbenzylamine (I) undergoes a two‐step substitution reaction with chloroacetyl chloride (II) and the aminoacetaldehyde acetal (III) to form the amide (IV) which is cyclized upon acidic treatment, yielding the tetracyclic piperazinone (V).
AGILD Seismic Modeling For Double-porosity Media (2008)

In this paper, we present the seismic fleld modeling for double-porosity media using AGILD method. The seismic difierential integral equations are discretized in the boundary strip zone by collocation FEM method. The seismic Galerkin equations are discretized by FEM method in internal domain. Both integral discretization on the strip zone and Galerkin FEM in the internal domain are used to construct AGILD seismic wave fleld modeling. The three phases' waveflelds are simulated through AGILD method in double-porosity media. 1. INTRODUCTION Studies on wave propagation in porous media have been carried out for decades. Recently, the major cause of ∞uid-induced porous rocks is analyzed in three difierent scales: macroscopic, mesoscopic, and microscopic (22{24). White (25) was the flrst to introduce the mesoscopic-loss mechanism based on the framework of Biot's theory. A patch saturation model was presented to describe the wave propagation character- istics in porous rocks partially saturated with water and gas. Carcione (8) numerically modeled the wavefleld in a porous media with mesoscopic gas pockets, and predicted higher attenuation than White's model due to multiple scattering and wave conversing. Carcione (9) investigated the White model and modeled difierent mesoscopic-scale heterogeneities and concluded that the loss because of ∞uid-modulus and porosity variations was the most important. Pride (24) used a unifled theoreti- cal treatment to describe three P wave attenuation models, which included a double-porosity model with a single ∞uid saturating, a patch-saturation model with two immiscible ∞uid and a microscopic squirt ∞ow model. Either of the two mesoscopic models produced attenuation in seismic band that was comparable to what was measured in the fleld. In this work, we investigate the double-porosity rocks (2,22{24) with a AGILD method calcu- lating the MFF pressure relaxation. Firstly, we simplify the double-porosity model with the same solid skeleton coupling with the two pore phases, and derive the displacement wave equations. Sec- ondly, we analyze the mesoscopic ∞uid ∞ow theory presented by Pride (22,23), derive the integral discretization to numerically solve the divergency increments caused by MFF. Finally, waveflelds are simulated.
Site of the Eu2+ Activator in the X-Ray Storage Phosphor SrFCl (1999)

With electron paramagnetic resonance (EPR) and electron nuclear double resonance (ENDOR) the surrounding of the activator Eu2+ was investigated in SrFCl single crystals. The analysis of the Eu2+ superhyperfine interaction with the nuclei of the next fluorine shells yielded that Eu2+ substitutes for Sr2+ and is not shifted from a regular lattice site. This result settles previous conflicting conclusions about such a shift.
Carbohydrate Supplementation During Prolonged Cycling Exercise Spares Muscle Glycogen But Does Not Affect Intramyocellular Lipid Use. (2007)

The effect of carbohydrate supplementation on muscle glycogen and intramyocellular triacylglycerol (IMTG) utilization during prolonged cycling is equivocal, as most studies have failed to observe muscle glycogen sparing or altered IMTG use. PURPOSE: Using contemporary stable isotope methodology and fluorescence microscopy, we assessed the impact of carbohydrate supplementation on whole-body and fiber-type specific IMTG and glycogen use during prolonged exercise. METHODS: Ten male subjects were studied twice during 3 h of cycling at 63±1% of maximal O2 uptake with continuous glucose ingestion (CHO trial; 0.7 g CHO · kg-1 · h-1) and without (CON trial; water only). Continuous infusions with [U-13C] palmitate and [6,6-2H2] glucose were applied to quantify plasma free fatty acid (FFA) and glucose oxidation rates and to estimate intramyocellular lipid and glycogen use. Before and after exercise muscle biopsy samples were taken to quantify fiber-type specific IMTG and glycogen content. RESULTS: Average plasma glucose rate of appearance (Ra) and disappearance (Rd) were 49±3 and 48±3% greater in the CHO compared to CON trial, respectively, and accompanied by a 16±4% greater plasma glucose oxidation rate throughout exercise. Carbohydrate supplementation resulted in lower muscle glycogen use during the first hour of exercise, resulting in a 38±12 and 57±14% lesser reduction in type I and II muscle fiber glycogen content in the CHO vs. CON trial, respectively. In the CHO trial both plasma FFA Ra and subsequent plasma FFA concentrations were lower, resulting in a 40±5% reduction in plasma FFA oxidation rates during exercise (P<0.05). Carbohydrate intake did not augment IMTG utilization, as fluorescence microscopy revealed a 76±7 and 78±7% reduction in type I muscle fiber lipid content in the CHO and CON trial, respectively. CONCLUSION: The lower total fat oxidation rate following carbohydrate supplementation during exercise is entirely attributed to reduced plasma FFA utilization, as muscle IMTG use is not affected by carbohydrate supplementation. Muscle glycogen sparing following carbohydrate supplementation occurs early in exercise. Therefore, athletes should initiate sports drink consumption immediately at the onset of exercise to maximize its effect on muscle glycogen sparing.
Antianginal effects of chromonar A combined investigation (1974)

The antianginal effect of chromonar was investigated in patients with coronary artery disease. Results are presented from twelve clinical departments in North Atnerica, all of which followed an identical protocol and test procedure. The results from these institutions illustrate some definite effects of chromonar. Using the attack rate, nitroglycerin requirement, and exercise tolerance test, chromonar was followed by a reduction in the number of attacks and nitroglycerin reqUirements. There was some carryover when the sequence chromonar‐placebo was used in the crossover design. Side effects were minimal; laboratory data indicated no tOXicity. Clinical conditions associated with coronary artery disease, such as hypertenSion and heart failure, were without influence on the effect of the drug. The data point toward an interesting field of research since the mechanism of the beneficial effect of chromonar in angina is obscure.
Effects of temperature on the hydrolysis of lactose by immobilized β‐galactosidase in a capillary bed reactor (1989)

The effects of temperature on the hydrolysis of lactose by immobilized beta-galactosidase were studied in a continuous flow capillary bed reactor. Temperature affects the rates of enzymatic reactions in two ways. Higher temperatures increase the rate of the hydrolysis reaction, but also increase the rate of thermal deactivation of the enzyme. The effect of temperature on the kinetic parameters was studied by performing lactose hydrolysis experiments at 15, 20, 25, 30, and 40 degrees C. The kinetic parameters were observed to follow an Arrhenius-type temperature dependence. Galactose mutarotation has a significant impact on the overall rate of lactose hydrolysis. The temperature dependence of the mutarotation of galactose was effectively modelled by first-order reversible kinetics. The thermal deactivation characteristics of the immobilized enzyme reactor were investigated by performing lactose hydrolysis experiments at 52, 56, 60, and 64 degrees C. The thermal deactivation was modelled effectively as a first order decay process. Based on the estimated thermal deactivation rate constants, at an operating temperature of 40 degrees C, 10% of the enzyme activity would be lost in one year.
Analysis of RNA by Northern-Blot Hybridization (2003)

Northern-blot hybridization, also referred to as Northern blotting, is one of several methods developed to detect the presence, to determine the size, and to quantify specific cellular mRNAs. By this method, total RNA or poly(A)(+)mRNA, prepared from the cells or tissue of interest, is fractionated by size on a denaturing agarose gel. The separated RNAs are transferred to a membrane by capillary action or under a vacuum and hybridized to a labeled probe with a base sequence complementary to all or part of the target mRNA. Analysis of hybridization signals determines whether the gene of interest is expressed in the cells or tissue, as well as the size and relative quantity of the target mRNA, if appropriate markers are used.
Cumate-Inducible Gene Expression System for Sphingomonads and Other Alphaproteobacteria (2013)

Tunable promoters represent a pivotal genetic tool for a wide range of applications. Here we present such a system for sphingomonads, a phylogenetically diverse group of bacteria that have gained much interest for their potential in bioremediation and their use in industry and for which no dedicated inducible gene expression system has been described so far. A strong, constitutive synthetic promoter was first identified through a genetic screen and subsequently combined with the repressor and the operator sites of the Pseudomonas putida F1 cym/cmt system. The resulting promoter, termed PQ5, responds rapidly to the inducer cumate and shows a maximal induction ratio of 2 to 3 orders of magnitude in the different sphingomonads tested. Moreover, it was also functional in other Alphaproteobacteria, such as the model organisms Caulobacter crescentus, Paracoccus denitrificans, and Methylobacterium extorquens. In the noninduced state, expression from PQ5 is low enough to allow gene depletion analysis, as demonstrated with the essential gene phyP of Sphingomonas sp. strain Fr1. A set of PQ5-based plasmids has been constructed allowing fusions to affinity tags or fluorescent proteins.
Phase 1b/2 study of olaratumab plus gemcitabine and docetaxel for the treatment of advanced soft tissue sarcoma (STS) (ANNOUNCE 2): Phase 1b results. (2018)

11542 Background: Olaratumab (O) is an antibody against platelet-derived growth factor receptor alpha. In a randomized phase 2 study, O in combination with doxorubicin (dox) demonstrated a significant improvement of overall survival (OS) over dox alone in patients (pts) with advanced STS. Here we report the safety, tolerability and recommended phase 2 dose (RPTD) of O plus gemcitabine (G) and docetaxel (D) (O + G/D). Methods: This dose-escalation study enrolled pts with advanced/metastatic STS, ≤2 prior lines of systemic therapy, no prior G, D or O, and ECOG PS 0-1. Pts received O on Days 1 and 8 at 15 mg/kg (cohort 1) or 20 mg/kg (cohort 2) with G (900 mg/m2 Days 1 and 8) and D (75 mg/m2 Day 8) on a 21-day cycle. The primary objective was to determine the RPTD of O + G/D, with a dose-limiting toxicity (DLT) occurring in Cycle 1 at a rate below 33%. Secondary objectives included safety and pharmacokinetics (PK). Results: 54 pts (cohort 1/2 = 21/33) received at least one dose of treatment. No DLT occurred in cohort 1. In cohort 2, 5 pts (15.2%) experienced 6 DLTs (ALT increase, bacteremia, neutropenia [2 pts], and thrombocytopenia [2pts]). Treatment-related adverse events (TRAEs) reported for cohorts 1 and 2 included all grades (90.5% and 93.9%), Gr 3 (14.3% and 42.4%), Gr 4 (0 and 18.2%), and serious AEs (9.5% and 15.2%), respectively. Common TRAEs (all grades, Gr ≥3) occurring in ≥ 25% of pts were fatigue (66.7%, 11.1%), anemia (61.1%, 18.5%), thrombocytopenia (35.2%, 18.5%), nausea (29.6%, 0%) and diarrhea (27.8%, 3.7%). 1 pt discontinued due to a study-related AE of fatigue (cohort 1). Following 2 deaths unrelated to study treatment, cohort 2 was expanded to 33 pts. The PK profile of O + G/D was similar to that of O in combination with other chemotherapies. Conclusions: Both dose levels were tolerated with a higher incidence for known toxicities of G/D in cohort 2. Based on safety and exposure-response analyses across O studies, the RPTD for O + G/D is 20 mg/kg at Days 1 and 8 in Cycle 1, followed by 15 mg/kg at Days 1 and 8 thereafter. The randomized, double-blinded phase 2 part of the study is enrolling and will compare OS of pts with STS receiving G/D + O vs G/D + placebo (ANNOUNCE 2). Clinical trial information: NCT02659020.
Examining the feasibility of face gesture detection using a wheelchair mounted camera (2009)

he user interface of existing autonomous wheelchairs concentrates on direct control of the wheelchair by the user using mechanical devices or various hand, head or face gestures. However, it is important to monitor the user to ensure safety an comfort of the user, who operates the autonomous wheelchair. In addition, such monitoring of a user greatly improves usablity of an autonomous wheelchair due to the improved communication between the user and the wheelchair. This paper proposes a user monitoring system for an autonomous wheelchair. The feedback of the user and the information about the actions of the user, obtained by such a system, will be used by the autonomous wheelchair for planning of its future actions. As a first step towards creation of the monitoring system, this work proposes and examines the feasibility of a system that is capable of recognizing static facial gestures of the user using a camera mounted on a wheelchair. The prototype of such a system has been implemented and tested, achieving 90% recognition rate with 6% false positive and 4% false negative rates.
Electrical and dielectric properties of bovine trabecular bone relationships with mechanical properties and mineral density (2003)

Interrelationships of trabecular bone electrical and dielectric properties with mechanical characteristics and density are poorly known. While electrical stimulation is used for healing fractures, better understanding of these relations has clinical importance. Furthermore, earlier studies have suggested that bone electrical and dielectric properties depend on the bone density and could, therefore, be used to predict bone strength. To clarify these issues, volumetric bone mineral density (BMDvol), electrical and dielectric as well as mechanical properties were determined from 40 cylindrical plugs of bovine trabecular bone. Phase angle, relative permittivity, loss factor and conductivity of wet bovine trabecular bone were correlated with Young's modulus, yield stress, ultimate strength, resilience and BMDvol. The reproducibility of in vitro electrical and dielectric measurements was excellent (standardized coefficient of variation less than 1%, for all parameters), especially at frequencies higher than 1 kHz. Correlations of electrical and dielectric parameters with the bone mechanical properties or density were frequency-dependent. The relative permittivity showed the strongest linear correlations with mechanical parameters (r > 0.547, p < 0.01, n = 40, at 50 kHz) and with BMDvol (r = 0.866, p < 0.01, n = 40, at 50 kHz). In general, linear correlations between relative permittivity and mechanical properties or BMDvol were highest at frequencies over 6 kHz. In addition, a significant site-dependent variation of electrical and dielectric characteristics, mechanical properties and BMDvol was revealed in bovine femur (p < 0.05, Kruskall-Wallis H-test). Based on the present results, we conclude that the measurement of electrical and dielectric properties provides quantitative information that is related to bone quantity and quality.
A genome-wide scan for the hereditary cutaneous melanoma in the MeLiM swine model (2004)

"Chantier qualité spécifique "Auteurs Externes" département de Génétique animale : uniquement liaison auteur au référentiel HR-Access "
Conformational analysis of bradykinin by annealed molecular dynamics and comparison to NMR‐derived conformations (1993)

Abstract Conformational analysis of bradykinin (BK), a nonapeptide of the sequence RPPGFSPFR, was accomplished using annealed molecular dynamics (AMD) at 1000 K in BIOGRAF 2.2. One hundred anneal cycles produced 100 conformations over approximately 2000 ps. These conformations were compared to structures derived by nuclear magnetic resonance (NMR) methods for similar shape and energy. Energy minimization of relevant conformations using both BIOGRAF 2.2 and AMBER 3.0a revealed that the AMD‐determined conformations are in the same energy range as the NMR‐determined structures. Also, the shape of the relevant conformations appeared similar, suggesting that AMD is a good tool for the conformational analysis of small peptide ligands. © 1993 John Wiley & Sons, Inc.
Salmonella vaccine carrier strains: effective delivery system to trigger anti-tumor immunity by oral route (1999)

Recombinant Salmonella strains expressing heterologous antigens can be delivered by oral route triggering the elicitation of efficient antigen-specific humoral, T helper and cytotoxic responses. The potential of attenuated Salmonella spp. to trigger anti-tumor immunity was evaluated for the first time by using β-galactosidase (β-gal) as a model tumor-associated antigen (TAA). β-gal was expressed in a Salmonella typhimurium aroA vaccine carrier strain either constitutively or under the control of a promoter activated upon infection. Oral immunization with both vaccine prototypes resulted in the elicitation of β-gal-specific humoral and cell-mediated immunity. Although both strains were able to trigger antigen-specific CTL, responses were more efficient when the expression was controlled by the promoter activated upon infection. The anti-tumor efficacy of the stimulated response was validated by challenging vaccinated animals with an aggressive fibrosarcoma transfected with β-gal, which operationally acts as a TAA. Both groups of vaccinated mice exhibited a significant reduction in tumor take and growth with respect to animals vaccinated with plasmidless carrier ( p < 0.05). However, the overall efficiency was better in the group in which β-gal was controlled by the in vivo -activated promoter (85 % versus 54 %; p < 0.05).
Preparation and investigation of acetyl salicylic acid-caffeine complex for rectal administration (2009)

An acetyl salicylic acid-caffeine complex was prepared and evaluated for the potential use in rectal administration. The results revealed the formation of a complex between acetyl salicylic acid and caffeine in a 1:1 molar ratio by a charge transfer mechanism. The effects of acetyl salicylic acid and complex on the rectal tissues showed destruction in the mucosal epithelium in case of acetyl salicylic acid; however, no change in the rectal tissues was noticed upon the administration of the complex. The effect of suppository bases on the release of the complex was studied using Witepsol H15 as fatty base and polyethylene glycols (PEG) 1000 and 4000 as a water soluble suppository base. The release profiles of acetyl salicylic acid and the complex were faster from PEG than from that of Witepsol H15. The percent release for the complex and acetyl salicylic acid from PEG base were 45.8, and 34.9%, respectively. However, it was 8.7 and 7.8%, respectively, from Witepsol H15 fatty base. The release kinetic was found to follow the non-Fickian diffusion model for complex from the suppository bases. It was concluded that acetyl salicylic acid caffeine complex can be used safely for rectal administration.
AC and DC electrochemical investigation of α-zirconium phosphate intercalated with hexamethylenetetramine (1995)

The ac-conductivity of α-zirconium phosphate intercalated with hexamethylenetetramine, α-Zr(HPO4)2·nH2O·0.26(CH2)6N4·0.48NH3, has been measured as a function of temperature (–20 to 25 °C) and relative humidity (11–90%) and compared with that of the host compound under the same experimental conditions. The activation energy for conduction increases with decreasing relative humidity from 0.52 to 0.71 eV. Emf measurements suggested that the main charge carriers are protonic species other than protons or hydrated protons. Mass spectrometry coupled with dc-conductivity experiments gave evidence that the intercalation compound is, to some extent, an ammonium ion conductor and that hexamethylenetetramine is oxidized at the anode to yield CO2.