gebhart/patrepeval huggingface dataset:
Analysis of the interaction between RGD-expressing adenovirus type 5 fiber knob domains and αvβ3 integrin reveals distinct binding profiles and intracellular trafficking (2006)

Adenovirus (Ad) vectors are used widely for experimental and therapeutic gene transfer. Ad-mediated gene delivery is often inefficient and, thus, there is considerable interest in developing Ad vectors that overcome biological barriers to efficient virus uptake. For this strategy to succeed, it is imperative that the interaction between such Ad vectors and their novel receptors is well understood. In this study, three surface-exposed loops (HI, CD and IJ loops) on the Ad5 fiber knob domain were selected as sites for insertion of an alphavbeta3 integrin-binding RGD sequence. Three RGD-containing Ad5 fiber knob-domain mutants were produced as recombinant proteins and all were shown to interact with soluble alphavbeta3 integrin by using biomolecular cell-free assays. Cell adsorption and subsequent internalization and intracellular trafficking of each of these proteins were assessed by confocal microscopy. Whilst the Ad5 fiber knob domain expressing the RGD sequence in the HI and CD loops bound with similar association and dissociation profiles, the fiber knob domain expressing the RGD sequence in the IJ loop bound with slower association and faster dissociation rates. By using molecular modelling, it was shown that the Ad5 fiber knob domain in which the RGD peptide was expressed in the IJ loop was only capable of binding to one alphavbeta3 integrin molecule per trimer. In contrast, fiber knob domains in which the RGD peptide was expressed in the HI and CD loops were capable of binding to one integrin molecule per monomer. These differences in the interactions between each mutant and alphavbeta3 may explain our observation that the three RGD-bearing Ad5 fiber knob domains demonstrated similar internalization rates, but distinct patterns of endosomal transport and escape.
Characterization of the gene encoding glyoxalase II from <i>Leishmania donovani</i>: a potential target for anti-parasite drugs (2005)

The glyoxalase system is a ubiquitous detoxification pathway that protects against cellular damage caused by highly reactive oxoaldehydes such as methylglyoxal which is mainly formed as a by-product of glycolysis. The gene encoding GLOII (glyoxalase II) has been cloned from Leishmania donovani, a protozoan parasite that causes visceral leishmaniasis. DNA sequence analysis revealed an ORF (open reading frame) of approximately 888 bp that encodes a putative 295-amino-acid protein with a calculated molecular mass of 32.5 kDa and a predicted pI of 6.0. The sequence identity between human GLOII and LdGLOII (L. donovani GLOII) is only 35%. The ORF is a single-copy gene on a 0.6-Mb chromosome. A approximately 38 kDa protein was obtained by heterologous expression of LdGLOII in Escherichia coli, and homogeneous enzyme was obtained after affinity purification. Recombinant L. donovani GLOII showed a marked substrate specificity for trypanothione hemithioacetal over glutathione hemithioacetal. Antiserum against recombinant LdGLOII protein could detect a band of anticipated size approximately 32 kDa in promastigote extracts. By overexpressing the GLOII gene in Leishmania donovani using Leishmania expression vector pspalphahygroalpha, we detected elevated expression of GLOII RNA and protein. Overexpression of the GLOII gene will facilitate studies of gene function and its relevance as a chemotherapeutic target. This is the first report on the molecular characterization of glyoxalase II from Leishmania spp. The difference in the substrate specificity of the human and Leishmania donovani glyoxalase II enzyme could be exploited for structure-based drug design of selective inhibitors against the parasite.
UV tuning of twin-core-fiber demultiplexers (1995)

Inexpensive demultiplexers for the 1.3- and 1.5-μm windows are essential components in passive optical networks, and the widespread use of analog (rather than digital) cable television necessitates an isolation of at least 20 dB (optical) between the windows. We report a compact optical-to-electrical demultiplexing detector with greater than 27-dB isolation, with 1.3/1.5-μm signals split by a twin-core fiber (TCF) and detected by an InGaAs bi-cell array (Fig. 1). The wavelength response measured electrically with the InGaAs array closely follows that measured optically.
Indole Synthesis via Rhodium Catalyzed Oxidative Coupling of Acetanilides and Internal Alkynes (2010)

ADVERTISEMENT RETURN TO ISSUEPREVAddition/CorrectionNEXTORIGINAL ARTICLEThis notice is a correctionIndole Synthesis via Rhodium Catalyzed Oxidative Coupling of Acetanilides and Internal AlkynesDavid R. Stuart, Mégan Bertrand-Laperle, Kevin M. N. Burgess, and Keith Fagnou*Cite this: J. Am. Chem. Soc. 2010, 132, 50, 17973Publication Date (Web):November 24, 2010Publication History Published online24 November 2010Published inissue 22 December 2010https://pubs.acs.org/doi/10.1021/ja108266qhttps://doi.org/10.1021/ja108266qcorrectionACS PublicationsCopyright © 2010 American Chemical Society. This publication is available under these Terms of Use. Request reuse permissions This publication is free to access through this site. Learn MoreArticle Views3385Altmetric-Citations2LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail PDF (62 KB) Get e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Chemical structure,Hydrocarbons,Indoles,Oxidative coupling,Rhodium Get e-Alerts
Phase Conjugation and Real Image Projection (2007)

This chapter contains sections titled: Real Image Projection Techniques Phase Conjugation–a Descriptive Approach Perfect Conjugate Illumination (Examples) Collimator Choices Perfect Conjugate Illumination (More Examples) Effects of Imperfect Conjugates Image Location (Analytical) Image Magnification Relation to the Lens and Prism-Pair Model Image Aberrations — Astigmatism Conclusions References
Heterozygote excess is repeatedly observed in females at the BRCA2 locus N372H (2004)

<h3>Abstract</h3> Geographically isolated populations, including island-mainland populations, tend to exhibit phenotypic variation in many species. The so-called island syndrome occurs when different environmental pressures lead to insular divergence from mainland populations. This phenomenon can be seen in an island population of Nova Scotia masked shrews (<i>Sorex cinereus</i>), which have developed a specialized feeding habit and digestive enzyme compared to their mainland counterparts. Epigenetic modifications, such as DNA methylation (DNAm), can impact phenotypes by altering gene expression without changing the DNA sequence. Here, we used a <i>de novo</i> masked shrew genome assembly and epigenome-wide assay to investigate morphological and DNA methylation patterns between island and mainland populations. Island shrews were morphologically and epigenetically different than their mainland counterparts, exhibiting a smaller body size; remarkably, the gene ontology analyses mirrored these phenotypes including genes for the digestive enzyme phenotypes. Moreover, island shrews appeared to age faster than their mainland counterparts. This study provides an example counter to the prevailing island syndrome morphological patterns, along with novel epigenomic insight into the drivers of island-mainland divergence in mammals.
Large Area Micro-Channel Plates for LAPPDTM (2014)

Manufacturing plans for “next generation” microchannel plates (MCPs) and the technical advantages enabled by this evolving technology are presented. The Large Area Picosecond Photodetector (LAPPD) is an MCP based photodetector, capable of imaging, with high spatial and temporal resolution in a hermetic package with an active area of 400 square centimeters. A key component of LAPPD is a chevron pair of large area (20 x 20 cm) MCPs. The manufacture of these large-area high performance MCPs has been enabled by the convergence of two technological breakthroughs. The first is the ability to produce large blocks of hollow, micronsized glass capillary arrays (GCAs) developed by Incom Inc. The Incom process is based on the use of an etchless “hollow-core” approach in the glass drawing process, eliminating the need to remove core material by chemical etching. The arrays are fabricated as large blocks that can be sliced to form large area wafers, without regard to the conventional limits of L/d (capillary length / pore diameter). Moreover, the glass used in these GCAs is physically more robust, does not have a tendency to warp, and has low levels of radioactive isotopes resulting in low dark noise. The second breakthrough is the advent of atomic layer deposition (ALD) coating methods and materials to functionalize GCAs to impart the necessary resistive and secondary emission properties suitable for large area detector applications. Recent results demonstrating the high performance, uniformity, and long term stability of the current MCP product are presented.
A boiling point model to describe conducting organic polymer gas sensor responses (2001)

Through assessment of the steady-state electrical resistance changes of poly(3,3-didecyl-2,2:5',2-terthiophene) doped with various counter ions upon exposure to multiple concentrations of 27 volatile organic chemicals, it has been shown that a linear relationship exists between log(1/C-v) and T-b (C,, being the analyte concentration required to generate a fixed sensor response, and Tb being analyte boiling point) for analytes which share a common functional group. The study supports a physico-chemical model of conducting organic polymer gas sensor responses based on non-specific partition of the analyte between vapour and polymer phases.
A multi‐dimensional analysis of cue‐elicited craving in heavy smokers and tobacco chippers (2001)

Aims . This research examined the performance of a broad range of measures posited to relate to smoking craving. Design . Heavy smokers and tobacco chippers, who were either deprived of smoking or not for 7 hours, were exposed to both smoking (a lit cigarette) and control cues. Participants . Smokers not currently interested in trying to quit smoking ( n = 127) were recruited. Heavy smokers ( n = 67) averaged smoking at least 21 cigarettes/day and tobacco chippers ( n = 60) averaged 1‐5 cigarettes on at least 2 days/week. Measurements . Measures included urge rating scales and magnitude estimations, a rating of affective valence, a behavioral choice task that assessed perceived reinforcement value of smoking, several smoking‐related judgement tasks and a measure of cognitive resource allocation. Findings . Results indicated that both deprivation state and smoker type tended to affect responses across these measurement domains. Conclusions . Findings support the use of several novel measures of craving‐related processes in smokers.
Secreted<i>Bacillus anthracis</i>proteases target the host fibrinolytic system (2011)

The fibrinolytic system is often the target for pathogenic bacteria, resulting in increased fibrinolysis, bacterial dissemination, and inflammation. The purpose of this study was to explore whether proteases NprB and InhA secreted by Bacillus anthracis could activate the host's fibrinolytic system. NprB efficiently activated human pro-urokinase plasminogen activator (pro-uPA), a key protein in the fibrinolytic cascade. Conversely, InhA had little effect on pro-uPA. Plasminogen activator inhibitors (PAI)-1, 2 and the uPA receptor were also targets for NprB in vitro. InhA efficiently degraded the thrombin-activatable fibrinolysis inhibitor (TAFI) in vitro. Mice infected with B. anthracis showed a significant decrease in blood TAFI levels. In another mouse experiment, animals infected with isogenic inhA deletion mutants restored TAFI levels, while the levels in the parent strain decreased. We propose that NprB and InhA may contribute to the activation of the fibrinolytic system in anthrax infection.
Expression of Acidic and Basic Fibroblast Growth Factors in Human and Bovine Vascular Smooth Muscle Cells (1990)

The expression and synthesis of acidic and basic fibroblast growth factors (aFGF and bFGF) in cultures of bovine and human vascular smooth muscle cells (BSMC and HSMC) was studied. BSMC express and synthesize only bFGF, whereas HSMC express and synthesize both bFGF and aFGF. The presence of bFGF in BSMC is shown by the following criteria: (1) the growth factor activity in BSMC lysates binds to a heparin-affinity column and elutes as a single peak at 1.5-1.7 m NaCl, characteristic for bFGF; (2) this extract is mitogenic for smooth muscle cells; (3) Northern blot analysis demonstrates three distinct bFGF mRNAs of 7.0, 4.0, and 1.9 kb; no aFGF mRNA species were detected. Analysis of human umbilical vein endothelial cells yielded similar results: Heparin-affinity chromatography and Northern blot analysis failed to demonstrate the presence of aFGF despite the detection of bFGF by these techniques. In contrast, HSMC synthesize two growth factor activities: First, they bind to an immobilized heparin column and elute as two separate peaks at 1.2 and 1.5-1.7 m NaCl, characteristic for aFGF and bFGF; and second, Northern blot analysis demonstrates the expression of aFGF mRNA of 4.6 kb and bFGF mRNAs of 7.0, 4.0 and 1.9 kb. Furthermore, it is shown that aFGF and bFGF are potent mitogens for smooth muscle cells in vitro.
Dielektrische Messungen an polaren Gemischen. 3. Mitteilung. Thermodynamische Theorie der Solvatation kugelförmiger Ionen in binären Lösungsmittelgemischen (1955)

Abstract Es wird mit Hilfe der Elektrostatik der Kontinua und einer Reihe von vereinfachenden Voraussetzungen ein Ausdruck für die freie Energie eines binären Lösungsmittelgemisches, das unipolare Ionen enthält, aufgestellt. Die Lösung, die mittels Variationsrechnung und eines Grenzüberganges für unendliche Verdünnung gefunden wird, zeigt, dass in der Umgebung des Ions die Lösungsmittelkom‐ponente mit der grösseren dielektrischen Suszeptibilität angereichert wird. Der berechnete Feldeffekt in Wasser‐Dioxan ist um ca. eine Grössenordnung zu klein, um die früher beobachteten Resultate zu erklären. Daher kann auf eine Mithilfe sterischer Effekte bei der auswählenden Solvatation geschlossen werden.
The Tensile Strength and Tacky Behavior of Polymeric Liquids (1960)

First Page
A Convenient Synthetic Route to 4-Aryl-1,4-dihydro-3(2<i>H</i>)-isoquinolinones (1987)

The title compounds are synthesized by Friedel-Crafts cyclization of N-benzyl-α-chloroacetamides, derived from the dichlorocarbene adducts of benzaldehyde N-benzylimines.
PsPM-RRM1-2: SCR, ECG and respiration measurement in response to electric stimulation or visual targets (2019)

This dataset includes skin conductance response (SCR), electrocardiogram (ECG) and respiration measurements for each of 29 healthy unmedicated participants (7 males and 22 females aged 23.5 +/- 3.6 years) in response to 10 discomforting electric stimulations to the forearm (RRM1) or 10 visual targets in a visual detection task (RRM2). The sample partly overlaps with data set PsPM-FR. Some participants did not take part in RRM1 or RRM2 such that there are 25 recordings for RRM1 and 26 recordings for RRM2. Electric shock stimuli are 0.2 ms wide square current pulse repeated at 500 Hz for 500 ms and individually adjusted amplitude just below the pain threshold. Visual stimuli are red crosses (+) embedded in a white digit stream; each stimulus is presented during 200 ms and separated by a 800 ms blank interval. ITI is selected randomly on each trial from 40 s, 45 s or 50 s. A baseline period with distractors but no targets concludes experiment RRM2. (This is in contrast to the methods description in Bach et al. (2016), according to which the baseline period was randomly either in the beginning or at the end of the experiment. This discrepancy was caused by an error in the code that controlled the experiment presentation.)
Structure of the Nonclassical 7-Norbornenyl Cation<sup>1</sup> (1966)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStructure of the Nonclassical 7-Norbornenyl Cation1M. Brookhart, Arthur Diaz, and S. WinsteinCite this: J. Am. Chem. Soc. 1966, 88, 13, 3135–3136Publication Date (Print):July 1, 1966Publication History Published online1 May 2002Published inissue 1 July 1966https://pubs.acs.org/doi/10.1021/ja00965a046https://doi.org/10.1021/ja00965a046research-articleACS PublicationsRequest reuse permissionsArticle Views643Altmetric-Citations35LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
A fast recursive algorithm for optimum sequential signal detection in a BLAST system (2004)

BLAST (Bell Laboratories layered Space-Time) wireless systems are multiple-antenna communication schemes which can achieve very high spectral efficiencies in scattering environments, with no increase in bandwidth or transmitted power. The most popular and, by far, the most practical architecture is the so-called vertical BLAST (V-BLAST). The signal detection algorithm of a V-BLAST system is computationally very intensive. If the number of transmitters is M and is equal to the number of receivers, this complexity is proportional to M/sup 4/ at each sample time. In this paper, we propose a simple and very efficient algorithm that reduces the complexity by a factor of M.
Effects of increased transaldolase activity on D-xylulose and D-glucose metabolism in Saccharomyces cerevisiae cell extracts (1991)

In vitro metabolism of D-xylulose and D-glucose in extracts obtained from D-glucose- and D-xylulose-fermenting Saccharomyces cerevisiae cells was investigated with 10- and 100-fold-increased activity of the enzyme transaldolase (EC 2.2.1.2). The rate of sugar consumption was the same in most cases, whereas the rate of ethanol formation decreased with increased levels of transaldolase. The formation of glycerol, pentitols, and acetic acid was not dependent on added transaldolase but was dependent on the sugar used as the growth substrate and on the sugar used in the in vitro metabolism experiments. The carbon balance showed that the dissimilated carbon could not be accounted for in products when transaldolase was added. The concentration of D-fructose-1,6.-diphosphate in the extracts was not influenced by added transaldolase but was higher with D-xylulose than with D-glucose. Levels of pyruvate, comparable with the two substrates, decreased with increasing levels of transaldolase. Exogenously added transaldolase decreased D-sedoheptulose-7-phosphate levels when D-xylulose was the substrate. The results are discussed in relation to the dissimilation of carbon through the upper part of glycolysis and the pentose phosphate pathway.
Phonon diodes and transistors from magneto-acoustics (2014)

By sculpting the magnetic field applied to magneto-acoustic materials, phonons can be used for information processing. Using a combination of analytic and numerical techniques, we demonstrate designs for diodes (isolators) and transistors that are independent of their conventional, electronic formulation. We analyze the experimental feasibility of these systems, including the sensitivity of the circuits to likely systematic and random errors.
Efficient duck hepatitis B virus production by an avian liver tumor cell line (1990)

Duck hepatitis B virus (DHBV) is produced in small amounts following transfection of human hepatoma or hepatoblastoma cell lines with cloned viral DNA. In a search for better hosts for DHBV replication, two avian liver cell lines were investigated. One of these cell lines, LMH, produced 5 to 10 times more DNA replicative intermediates and 10 to 20 times more infectious DHBV than did either of the two human cell lines, HuH-7 and Hep G2. Utilization of cell lines in genetic analyses of virus replication is often dependent upon obtaining efficient complementation between cotransfected viral genomes. We assayed transcomplementation of a viral polymerase (pol) gene mutant, which is rather inefficient in transfected human cells, and found that viral DNA synthesis was at least 20 times more efficient following cotransfection of LMH cells than in similarly transfected HuH-7 cells. Recombination, a potential interpretation problem in complementation assays, occurred at low levels in the cotransfected cultures but was substantially reduced or eliminated by creation of an LMH subline stably expressing the viral polymerase. This cell line, pol-7, supported the replication of DHBV pol mutants at ca. 10 to 15% of the level of virus replication obtained following transfection with wild-type viral DNA. By transcomplementation of a pol gene mutant in LMH cells, we were able to produce sufficient virus with the mutant genome to investigate the role of polymerase in covalently closed circular DNA amplification. Our results substantiate the hypothesis that covalently closed circular DNA is synthesized by the viral reverse transcriptase.
Monaural and binaural processing for automatic estimation of room acoustics perceptual attributes (2001)

cote interne IRCAM: Baskind01a
Cytotoxicity Evaluation and Crystallochemical Analysis of a Novel and Commercially Available Bone Substitute Material (2015)

The novel composite demonstrated a high degree of cytocompatibility, which is promising in future clinical trials.