gebhart/patrepeval huggingface dataset:
Influence of coagulation factor 13A <i>(F13A)</i> gene variants on overall survival (OS) of ovarian cancer patients (2004)

5002 Background: Abnormalities of haemostasis, including hyper-coagulation and enhanced fibrinolysis, are evident in advanced epithelial ovarian cancer (EOC). Polymorphisms of the F13A gene influence both F13 enzyme activity, and characteristics of the cross-linked product. We therefore evaluated F13A genetic polymorphisms in patients with EOC. Methods: 260 peripheral blood DNAs, from EOC patients, were examined for five F13A polymorphisms: Val34Leu, Tyr204Phe and Pro564Leu by the TaqMan™ assay and Val650Ile and Glu651Gln by PCR-SSCP. Results: Median age was 61 (29–89). With a median follow-up of 38 months (range 265 months), median overall survival (OS) was 99 months. The frequency of each genotype did not differ from those found within the normal healthy population. No association was identified between stage at presentation and any of the five genotypes. Significant associations were identified between overall survival and genotype. 650Ile was associated with shorter OS (p=0.008 by logrank test). Carriage of the 34Leu allele was associated with longer OS in patients with FIGO stage III and IV (p=0.0219 by logrank test). When a multivariate cox regression model comprising stage, the Val650Ile and Val34leu polymorphisms was performed, these polymorphisms independently predicted OS (see table). Conclusion: F13A Val34Leu and Val650Ile genetic polymorphisms may be valuable prognostic factors in ovarian cancer patients. Author Disclosure Employment or Leadership Consultant or Advisory Stock Ownership Honoraria Research Funding Expert Testimony Other Remuneration Yorkshire Cancer Research; West Riding Medical Research Trust
2-Nitro-3-pivaloyloxypropene (2001)

[78551-14-1] C8H13NO4 (MW 187.22) InChI = 1S/C8H13NO4/c1-6(9(11)12)5-13-7(10)8(2,3)4/h1,5H2,2-4H3 InChIKey = HRYQXODNLXZZPD-UHFFFAOYSA-N (multicoupling reagent; reacts stepwise with two different nucleophiles1) Alternate Name: NPP. Physical Data: mp 18 °C; bp 40–45 °C/0.01 mmHg. Solubility: sol all organic solvents; decomposes in pyridine and slowly in protic solvents. Preparative Method: prepared in four steps from Formaldehyde, Nitromethane, and pivaloyl chloride (ca. 25% overall yield at a 0.7 mol scale).1 Handling, Storage, and Precautions: can be stored six months at −10 °C without decomposition. Use in a fume hood.
Replacing Metal-Based Lightning Strike Protection Layer of CFRPS by 3D Printed Electronically Conductive Polymer Layer (2019)

Electrically conductive adhesive layers were deposited on top of aerospace grade carbon fiber reinforced plastic (CFRP) panels using a small-scale 3D printer. Polylactic acid (PLA) filaments with copper filler (CU-PLA) and graphene filler (GO-PLA) were used to print around 0.7 mm thick electrically conductive layer on top of CFRP panels. 3D printed polymeric layers were tested for their effectiveness as a lightning strike protection (LSP) material by subjected to a simulated lightning strike. A painted, electrically non-conductive unprotected panel was also tested for comparison. In the case of the CU-PLA protected sample, a high electrical conductivity proved to be useful in fast dissipation of the lightning current. Fast current dissipation helped to reduce the resistive heating after a lightning strike. Thermography and high-speed camera analysis were employed to study the heat generation and current dissipation during the lightning strike. These results established that a useful Faraday Cage was applied via additive manufacturing successfully. This work shows the successful application of 3D printing for producing LSP technologies, with future work aimed at investigating optimal printable electrically conductive thermoset material candidates.
Equivalence of two theories of strong optical scintillation (1974)

We demonstrate that the theories of strong irradiance fluctuations of Yura and Clifford et al., although they start from slightly different physical models, produce the same predictions for the behavior of an optical wave propagating through strong turbulence. Both theories predict a saturation of the irradiance variance and that in the saturation regime the amplitude correlation length is equal to the lateral coherence length of the optical wave. The detailed structure of the amplitude correlation functions is also similar, showing a more-rapid decay at the origin and an enhanced tail at large separations in comparison to the results of the weak scattering theory.
Geography is essential for reproductive isolation between florally diversified morning glory species from Amazon canga savannahs (2019)

The variety, relative importance and eco-evolutionary stability of reproductive barriers are critical to understanding the processes of speciation and species persistence. Here we evaluated the strength of the biotic prezygotic and postzygotic isolation barriers between closely related morning glory species from Amazon canga savannahs. The flower geometry and flower visitor assemblage analyses supported pollination by the bees in lavender-flowered Ipomoea marabaensis and recruitment of hummingbirds as pollinators in red-flowered Ipomoea cavalcantei. Nevertheless, native bee species and alien honeybees foraged on flowers of both species. Real-time interspecific hybridization underscored functionality of the overlap in flower visitor assemblages, questioning the strength of prezygotic isolation underpinned by diversification in flower colour and geometry. Interspecific hybrids were fertile and produced offspring in nature. No significant asymmetry in interspecific hybridization and hybrid incompatibilities among offspring were found, indicating weak postmating and postzygotic isolation. The results suggested that despite floral diversification, the insular-type geographic isolation remains a major barrier to gene flow. Findings set a framework for the future analysis of contemporary evolution of plant-pollinator networks at the population, community, and ecosystem levels in tropical ecosystems that are known to be distinct from the more familiar temperate climate models.
Moteur électrique à fort couple massique pour l'aéronautique (2007)

International audience
PERFORMANCE AND APPLICATIONS OF RESIDENTIAL BUILDING ENERGY GREY-BOX MODELS (2013)

The electricity market is in need of a method to accurately predict how much peak load is removable by directly controlling residential thermostats. Utilities have been experimenting with residential demand response programs for the last decade, but inconsistent forecasting is preventing them from becoming a dependent electricity grid management tool. This dissertation documents the use of building energy models to forecast both general residential energy consumption and removable air conditioning loads. In the models, complex buildings are represented as simple grey-box systems where the sensible energy of the entire indoor environment is balanced with the flow of energy through the envelope. When internet-connected thermostat and local weather data are inputs, twelve coefficients representing building parameters are used to non-dimensionalize the heat transfer equations governing this system. The model's performance was tested using 559 thermostats from 83 zip codes nationwide during both heating and cooling seasons. For this set, the average RMS error between the modeled and measured indoor air temperature was 0.44°C and the average daily ON time prediction was 1.9% higher than the data. When combined with smart power meter data from 250 homes in Houston, TX in the summer of 2012 these models outperformed the best traditional methods by 3.4 and 28.2% predicting daily and hourly energy consumption with RMS errors of 86 and 163 MWh. The second model that was developed used only smart meter and local weather data to predict loads. It operated by correlating an effective heat transfer metric to past energy data, and even further improvement forecasting loads were observed. During a demand response trial with Earth Networks and CenterPoint Energy in the summer of 2012, 206 internet-connected thermostats were controlled to reduce peak loads by an average of 1.13 kW. The thermostat building energy models averaged forecasting the load in the 2 hours before, during, and after these demand response tests to within 5.9%. These building energy models were also applied to generate thermostat setpoint schedules that improved the energy efficiency of homes, disaggregate loads for home efficiency scorecards and remote energy audits, and as simulation tools to test schedule changes and hardware upgrades.
Inhibition of serine palmitoyltransferase delays the onset of radiation-induced pulmonary fibrosis through the negative regulation of sphingosine kinase-1 expression (2012)

The enforcement of sphingosine-1-phosphate (S1P) signaling network protects from radiation-induced pneumonitis. We now demonstrate that, in contrast to early postirradiation period, late postirradiation sphingosine kinase-1 (SphK1) and sphingoid base-1-phosphates are associated with radiation-induced pulmonary fibrosis (RIF). Using the mouse model, we demonstrate that RIF is characterized by a marked upregulation of S1P and dihydrosphingosine-1-phosphate (DHS1P) levels in the lung tissue and in circulation accompanied by increased lung SphK1 expression and activity. Inhibition of sphingolipid de novo biosynthesis by targeting serine palmitoyltransferase (SPT) with myriocin reduced radiation-induced pulmonary inflammation and delayed the onset of RIF as evidenced by increased animal lifespan and decreased expression of markers of fibrogenesis, such as collagen and α-smooth muscle actin (α-SMA), in the lung. Long-term inhibition of SPT also decreased radiation-induced SphK activity in the lung and the levels of S1P-DHS1P in the lung tissue and in circulation. In vitro, inhibition or silencing of serine palmitoyltransferase attenuated transforming growth factor-β1 (TGF-β)-induced upregulation of α-SMA through the negative regulation of SphK1 expression in normal human lung fibroblasts. These data demonstrate a novel role for SPT in regulating TGF-β signaling and fibrogenesis that is linked to the regulation of SphK1 expression and S1P-DHS1P formation.
Summary of the National Toxicology Program's report of the endocrine disruptors low-dose peer review. (2002)

At the request of the U.S. Environmental Protection Agency (U.S. EPA), the National Toxicology Program organized an independent and open peer review to evaluate the scientific evidence on low-dose effects and nonmonotonic dose-response relationships for endocrine-disrupting chemicals in mammalian species. For this peer review, "low-dose effects" referred to biologic changes that occur in the range of human exposures or at doses lower than those typically used in the standard testing paradigm of the U.S. EPA for evaluating reproductive and developmental toxicity. The demonstration that an effect is adverse was not required because in many cases the long-term health consequences of altered endocrine function during development have not been fully characterized. A unique aspect of this peer review was the willing submission of individual animal data by principal investigators of primary research groups active in this field and the independent statistical reanalyses of selected parameters prior to the peer review meeting by a subpanel of statisticians. The expert peer-review panel (the panel) also considered mechanistic data that might influence the plausibility of low-dose effects and identified study design issues or other biologic factors that might account for differences in reported outcomes among studies. The panel found that low-dose effects, as defined for this review, have been demonstrated in laboratory animals exposed to certain endocrine-active agents. In some cases where low-dose effects have been reported, the findings have not been replicated. The shape of the dose-response curves for reported effects varied with the end point and dosing regimen and were low-dose linear, threshold-appearing, or nonmonotonic. The findings of the panel indicate that the current testing paradigm used for assessments of reproductive and developmental toxicity should be revisited to see whether changes are needed regarding dose selection, animal-model selection, age when animals are evaluated, and the end points being measured following exposure to endocrine-active agents.
Metabolic Effects of IGF-I in Diabetic Rats (1991)

Insulinlike growth factor I (IGF-I) stimulates glucose utilization (GU) in nondiabetic rats. We compared the effects of IGF-I and insulin on glucose metabolism in control (fed plasma glucose 7.7 +/- 0.1 mM, n = 30) and partially (90%) pancreatectomized diabetic (plasma glucose 18.4 +/- 0.8 mM, n = 30) awake unstressed rats. IGF-I was infused at 0.65 or 1.96 nmol.kg-1.min-1 and insulin at 22 or 29 pmol.kg-1.min-1 in combination with [3-3H]glucose while euglycemia was maintained by a variable glucose infusion. In controls, GU during the 0.65- and 1.96-nmol.kg-1.min-1 IGF-I infusions (127 +/- 7 and 168 +/- 4 mumol.kg-1.min-1, respectively) was similar to rates observed during the 22- and 29-pmol.kg-1.min-1 insulin infusions (121 +/- 2 and 156 +/- 5 mumol.kg-1.min-1). Whole-body glycolytic rate (3H2O generation) and muscle glycogen synthetic rate were identical during insulin and IGF-I infusions. In diabetic rats, GU was reduced by 30% versus control rats (P less than 0.01) during both the low-dose (88 +/- 7 vs. 121 +/- 7 mumol.kg-1.min-1) and higher-dose (109 +/- 4 vs. 156 +/- 5 mumol.kg-1.min-1) insulin clamps. The defect in insulin action involved both muscle glycogen synthesis and glycolysis. In diabetic rats, IGF-I elicited rates of GU similar to controls (115 +/- 10 and 164 +/- 12 mumol.kg-1.min-1 during the 0.65- and 1.96-nmol.kg-1.min-1 infusions, respectively) and corrected the intracellular defects in glycogen synthesis and glycolysis.(ABSTRACT TRUNCATED AT 250 WORDS)
Natural Killer Cells: A Mystery No More (2002)

Recent years have witnessed remarkable progress in our understanding of the molecular mechanism regulating natural killer (NK) cell function. NK cells can sense whether cells have lost the surface expression of major histocompatibility complex (MHC)-class I molecules. The discovery of MHC-class I-specific inhibitory receptors clarified the basis of this discrimination and elucidated the nature of the 'off' signal. However, the receptors responsible for the 'on' signal in the process of natural cytotoxicity remained mysterious until recently. Here, we describe the identification and characterization of such receptors and discuss the emerging implications of these findings in different diseases.
Using Video Tutorials to Learn Maya 3D for Creative Outcomes (2016)

This study tracked the transition from traditional front-of-class software demonstration of Autodesk's Maya 3D to the introduction of video tutorials over a five-year period. It uses Mayer and Moreno's (2003) theory of multimedia learning to frame the analysis of results. It found that students' preference for the video tutorial increased over the course of the study. Students' preference for video tutorials was correlated with a reduction in cognitive load, increase in satisfaction with the learning experience and subsequent reduction in frustration with the software. While there was no apparent change in measurable outcomes, students' satisfaction rating with the video tutorial in preference to other learning media suggests more efficient learning was achieved. As a consequence of the findings, the traditional demonstration was discontinued. Overall, the introduction of video tutorials for learning Maya 3D reduced frustration and freed up time for more creative pursuits – the primary purpose for learning the software.
The Effect of Platelet-rich Fibrin Matrix on Rotator Cuff Healing in a Rat Model (2015)

The purpose of the current study was to determine if the application of platelet-rich fibrin matrix could improve regeneration of the tendon-bone insertion site in a rat rotator cuff repair model. 25 Lewis syngeneic rats underwent bilateral tenotomy and repair of the supraspinatus tendon. 10 separate rats were used for PRFM harvest. All left (control) shoulders underwent transosseous rotator cuff repair, while all right (treatment) shoulders were repaired similarly with PRFM augmentation. 9 rats were sacrificed at 2-weeks and ten at 4-weeks for biomechanical testing. 3 separate rats were sacrificed at 2-weeks and 4-weeks each for histologic analysis of the insertion site. At 2 weeks, the experimental group repairs were significantly stronger in ultimate load to failure (P=0.01), stress (P=0.03), and stiffness (P=0.03). Differences in biomechanical testing were not found between the groups at 4 weeks. Histological analysis revealed less collagen organization and cartilage formation at the insertion site in the experimental group. Semiquantitative histologic analysis confirmed our qualitative assessment of the specimens. PRFM does not recapitulate the native enthesis, but rather induces an exuberant and disordered healing response that is characterized by fibrovascular scar tissue.
Least-informative Bayesian prior distributions for finite samples based on information theory (2003)

A procedure, based on Shannon information theory, for producing least-informative prior distributions for Bayesian estimation and identification is presented. This approach relies on constructing an optimal mixture distribution and applies in small sample sizes. The procedure is illustrated in a small-scale numerical study and contrasted with an approach based on maximum entropy.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
3D ULTRASOUND: A NOVEL NEW IMAGING OPPORTUNITY FOR ORTHOPAEDIC SURGEONS (2019)

Introduction At present, orthopaedic surgeons utilize either CT, MRI or X-ray for imaging a joint. Unfortunately, CT and MRI are quite expensive, non weight-bearing and the orthopaedic surgeon does...
Durch sichtbares Licht induzierte Klick‐Chemie (2015)

Abstract Wir berichten über eine schnelle und katalysatorfreie Cycloaddition, die die Grundlage für lichtinduzierte Klick‐Chemie bildet. Hierfür wurde eine leicht zugängliche, photoaktive und auf 2 H ‐Azirinen basierende Spezies entwickelt, die Licht jenseits der Wellenlänge von 400 nm absorbiert. Die Bestrahlung mit einer niederenergetischen Lichtquelle ermöglicht daher die effiziente Synthese kleiner Moleküle durch Umsetzung einer Vielfalt an Mehrfachbindungskomponenten. Um die Effizienz des hier vorgestellten Ansatzes zu demonstrieren, wurde der photoaktivierbare Chromophor mit funktionalisierten polymeren Substraten umgesetzt, wobei bei Raumtemperatur vollständige Ligation in nur einer Minute Bestrahlungszeit erreicht wurde. Wir stellen somit eine höchst effiziente Methode vor, die sich für Anwendungen eignet, bei denen eine selektive Cycloaddition an Materialien mit elektronenarmen Mehrfachbindungen gefordert ist.
Divergent Thinking in Parkinsonism: A Case–Control Study (2017)

In the present study, ATTA was significantly lower in PSP patients than in the other study groups. The worst performance in ATTA-total score and the lower score in FAB in PSP patients support the role of frontal function in creative processes.
Reactivity and Structural Diversity in the Reaction of Guanidine 1,5,7‐Triazabicyclo[4.4.0]dec‐5‐ene with CO<sub>2</sub>, CS<sub>2</sub>, and Other Heterocumulenes (2016)

The guanidine 1,5,7‐triazabicyclo[4.4.0]dec‐5‐ene (TBD) reacted with the heterocumulenes CO 2 , CS 2 , i Pr 2 NCN i Pr 2 , p ‐tolylNCO, and p ‐tolylNCS to give three types of products: TBD–CO 2 ( 1 ) and TBD– p ‐tolylNCS ( 5 ), resulting from the addition of the cumulene to TBD, TBD(–H)({ i PrNH}–C=N{ i Pr}) ( 3 ) and TBD(–H)( p ‐tolylNHCO) ( 4 ), resulting from addition of the N–H bond of TBD to the C=N bond of the cumulene, and the dithiocarbamate salt [TBDH][TBD(–H)CS 2 ] ( 2 ) after transfer of a proton to a second molecule of TBD. Competition reactions indicated that the stability of the compounds decreases in the order 4 > 5 > 1 > 2 > 3 . DFT calculations showed that the relative stabilities of the products are governed by both the Lewis acidity of the heterocumulene and the Brønsted basicity of the TBD(–H)–cumulene anion, whereas their distinct structures are determined by the latter, with the most basic site governing the geometry of the final product.
Insertion of Cecropin A and reconstitution of bacterial outer membrane protein FhuA variants in polymeric membranes (2011)

Polymer based nanocompartments (polymersomes) have potential applications in synthetic biology (pathway engineering), medicine (drug release), and industrial biotechnology (chiral nanoreactors, multistep synthesis, bioconversions in non-aqueous environments, and selective product recovery). The aforementioned goals can be accomplished by polymer membrane functionalization through covalent bonding or inclusion of proteins/peptides, to obtain specific properties like recognition, catalytic activity and facilitated diffusion, mimicking the complexity of a biological membrane. Membrane protein FhuA Delta1-159 is already reported to be functionally reconstituted as channel protein into polymersome membranes of some polymers like PMOXA-PDMS-PMOXA. Nevertheless the hydrophobic mismatch, defined as the difference between the hydrophobic length of a membrane protein and the hydrophobic thickness of the membrane it spans, prevents the insertion of channel proteins like the engineered FhuA Delta1-159 into thick polymeric membranes. The first part of this thesis deals with addressing the challenge to minimize the hydrophobic mismatch between the channel protein and the polymeric membrane. A “rational” strategy to double the last five amino acids of each of the 22 beta-sheets present in FhuA Delta1-159 prior to the more regular periplasmatic beta-turns has been developed (FhuA Delta1-159 Ext). As a result the length of the calculated protein’s hydrophobic portion was increased by 1 nm. The secondary structure prediction and Circular Dichroism (CD) spectroscopy strongly suggest the beta-barrel structure of the engineered protein. The protein insertion and functionality within a nanocontainer polymeric membrane based on the triblock copolymer PIB1000-PEG6000-PIB1000 (PIB = polyisobutylene, PEG = polyethyleneglycol) has been proven by kinetic analysis using the HRP-TMB assay (HRP = Horse Radish Peroxidase, TMB = 3,3',5,5'-tetramethylbenzidine). Similar experiments with the parent FhuA Delta1-159 protein showed no insertion into the PIB1000-PEG6000-PIB1000 membrane. Furthermore labeling of the Lys-NH2 groups present in the FhuA Delta1-159 Ext channel, leads to controllability of in/out flux of substrates and products from the nanocontainer. The FhuA Delta1-159 Ext flexibility opens further possibilities to be applied as nano-channel in different block copolymer vesicles. In the thesis’s second part, a fusion protein (Cecropin A-EGFP) based on the Cecropin A antibacterial peptide and the EGFP (Enhanced Green Fluorescent Protein) was designed, expressed and biophysically characterized for the functionalization of a polymersome surface. The interaction with the tri-block PIB-PEG-PIB based polymersome membrane was analyzed by CD, coupled with EGFP and Trp fluorescence measurements. Results revealed that the Cecropin A peptide is inserted into the polymersome membrane mimicking its lipid bilayer behavior and acting as “membrane surface anchor” for water soluble proteins.