gebhart/patrepeval huggingface dataset:
A novel splice donor mutation in the thrombopoietin gene leads to exon 2 skipping in a Filipino family with hereditary thrombocythemia (2011)

Figure 1.THPO mutation inactivates the intron 2 splice donor and correlates with elevation of thrombopoietin level.(A) Pedigree of the 6 family members with serum thrombopoietin concentrations (pg/mL) in the 1st row and platelet counts (/mm 3 ) in the 2nd row.The proband (II-1) and her 2 children (III-1, III-2) exhibit thrombocytosis and high serum thrombopoietin levels.The proband's parents (I-1, I-2) and husband (II-2) have normal platelet counts and thrombopoietin levels.(B) A novel heterozygous T C point mutation at the splice donor site of THPO gene intron 2 was identified through Sanger sequencing in the proband and her children, but not in her parents or husband.(C) The serum thrombopoietin concentrations of family members with the THPO mutation were significantly higher than those family members without the mutation (P .01),or healthy controls (P .001).The thrombopoietin levels in the latter 2 groups showed no statistically significant difference.This analysis is performed with 1-way ANOVA followed by the Student-Newman-Keuls multiple comparisons test and the data are presented in the mean with SEM format.D) Basal phosphorylated STAT5 (pSTAT5) levels in CD3 /CD66 /CD14 myeloid progenitors were evaluated and the 95 th percentile is presented in the mean with SEM format.An un-paired t test revealed that pSTAT5 levels are significantly higher in the affected group (*P .024).(E) Top panel: schematic of the cloned inserts used for exon trapping.These inserts were amplified from patient genomic DNA, cloned into the pCR2.1-TOPOVector, and then subcloned into the pSPL3b exon trapping vector.Middle panel: expected splicing products from the constructs of the cloned sequence within pSPL3b.In the presence of a mutation in intron 2, exon 2 is expected to be spliced out of the resulting product.Bottom panel: electrophoretic visualization of cDNA-PCR products amplified from the constructs after transfection into COS-7 cells.RNA was extracted and reverse transcribed to cDNA 48 hours after the transfection of the pSPL3b-Insert construct into COS-7 cells.PCR was performed using primers SD6 and SA2, and products were resolved on a 2% agarose gel.Splicing of the vector alone yields a 261bp fragment resulting from the flanking vector exons.Splicing of the wild-type Insert 1 and Insert 2 constructs results in 547bp and 634 bp fragments, respectively.The mutant splice products display fragments that are 158bp shorter, indicating complete splicing-out of exon 2.
STRATEGIC MANAGEMENT BY THE ENTERPRICE APPROACH (2000)

This paper describes the use of intelligent technologies for traf information management adopted within the EU project ENTERPRICE, which has developed and evaluated the MObility and Traffic Information Centre (MOTIC) architecture, an open European architecture for a regional traffic information, mobility managem and supervision centre. The use of intelligent techniques in the context of MOTIC is shown the provision of a strategic traffic management shell (SMC) for traffic managers and planners, inclu tools for scenario simulation and analysis, strategy planning and dissemination to local information/control centres and operators. For the covering abstract see ITRD E114174.
On double-pulse stability criteria with damping (1952)

A simple necessary condition for the stability of a linear dynamic system with elastic and damping characteristics which vary periodically with the same period is derived. General explicit necessary and sufficient conditions for stability are then developed for a double-pulse system. Such a system can be characterized by a pair of eigenvalues, or complex frequencies, corresponding to each half-period, and the stability of this system depends only on these complex frequencies. It is shown that a necessary, though not sufficient, condition for the stability of any such system is that the arithmetic mean of the real parts of all four of the complex frequencies over an entire period be negative or zero. This is shown to be true, more generally, for an <inline-formula content-type="math/mathml"> <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" alttext="script n"> <mml:semantics> <mml:mrow class="MJX-TeXAtom-ORD"> <mml:mi class="MJX-tex-caligraphic" mathvariant="script">n</mml:mi> </mml:mrow> <mml:annotation encoding="application/x-tex">\mathcal {n}</mml:annotation> </mml:semantics> </mml:math> </inline-formula>-pulse system. The physical significance of the results is discussed, and numerical examples are given.
Vector Magnetic Property Measurement in Magnetic Steel Sheets under High Magnetic Flux Density Conditions (2011)

In order to develop higher torque, lower weight and smaller sized magnetic actuators for driving electrical vehicles and robot arms, designing under high magnetic field and high exciting voltage is most effective. It is therefore very important to understand vector magnetic properties in electrical steel sheets under high magnetic flux density conditions. The vector magnetic properties mean the relationship between the magnetic flux density vector and the magnetic field strength vector and it is very difficult to measure the each component under high magnetic flux density conditions because of the magnetic saturation and the magnetic anisotropy. In this paper, accurate measurement techniques of the vector magnetic properties over 1.5T and measured vector magnetic properties are reported and discussed.
Cisplatin adducts of DNA as precursors for nanostructured catalyst materials (2020)

The synthesis and characterisation of novel metal-modified DNA precursors for fuel cell catalyst development are described. Material precursors in the form of metal-DNA complexes were prepared through the reaction of DNA with cisplatin at various loadings and spectroscopically tested to confirm the platinum binding mode and the degree of complexation. The surface morphology of the DNA-metal material was analysed by Scanning Transmission Electron Microscopy (STEM), which revealed the extent of platinum nanocluster formation, with low metal loadings leading to observation of individual platinum atoms. Electrochemical measurements showed a greater electrocatalytic activity for the hydrogen evolution reaction (HER) with increased platinum loadings, shifting the half wave potential, <i>E</i> <sub>1/2</sub>, away from the glassy carbon limit towards that of a bulk Pt electrode. This is explained further by Tafel plots, from which a change in the mechanism of the apparent rate limiting step for proton reduction from a Volmer to a Heyrovsky mechanism is postulated as the platinum loading increases.
CARS temperature measurements in a hypersonic propulsion test facility (1990)

Nonintrusive diagnostic measurements were performed in the supersonic reacting flow of the Hypersonic Propulsion Test Cell 2 at NASA-Langley. A Coherent Anti-stokes Raman Spectroscopy (CARS) system was assembled specifically for the test cell environment. System design considerations were: (1) test cell noise and vibration; (2) contamination from flow field or atmospheric borne dust; (3) unwanted laser or electrically induced combustion (inside or outside the duct); (4) efficient signal collection; (5) signal splitting to span the wide dynamic range present throughout the flow field; (6) movement of the sampling volume in the flow; and (7) modification of the scramjet model duct to permit optical access to the reacting flow with the CARS system. The flow in the duct was a nominal Mach 2 flow with static pressure near one atmosphere. A single perpendicular injector introduced hydrogen into the flow behind a rearward facing step. CARS data was obtained in three planes downstream of the injection region. At least 20 CARS data points were collected at each of the regularly spaced sampling locations in each data plane. Contour plots of scramjet combustor static temperature in a reacting flow region are presented.
Cardiopulmonary Interactions (2016)

In the presence of underlying cardiac and respiratory disease, the interplay between these two systems is significant and plays a pivotal role in the pathophysiology of acute and chronic phases of a wide spectrum of diseases. An understanding of these relationships is essential to optimizing the care of critically ill patients.
Construction of fully symmetrical cubature rules of very high degree for the square (1993)

A new method for the construction of fully symmetrical cubature formulae for the square is proposed. Even for high degrees the formulae are surprisingly easy to compute: the hardest computation to perform is the solution of a system of two polynomial equations of relatively low degree in two unknowns.
The J300 Family of Video and Audio Adapters: Architecture and Hardware Design (1995)

family of video and audio adapters was to provide the hardware support necessary to allow the integration of broadcast video into workstations. The three primary objectives were as follows: (1) digitized video data should be treated the same as any other data type in the system; (2) the video and the graphics subsystem designs should be completely independent of each other; and (3) any hardware designed should be low cost. Digital has implemented the J300 architecture in three products: Sound & Motion J300, FullVideo Supreme JPEG, and FullVideo Supreme. The Sound & Motion J300 (referred to in this paper simply as the J300) was the first product designed with this architecture and is the primary focus of this paper. The FullVideo Supreme JPEG and FullVideo Supreme products are based on the same design database as the J300. They differ from the J300 in the bus supported (they support the peripheral component interconnect [PCI] bus) and the lack of audio support. Additionally, the FullVideo Supreme product does not include hardware compression/decompression circuitry. The J300 brings a wide range of video and audio capabilities to machines based on Digital’s TURBOchannel I/O interconnect. Analog broadcast video can be digitized, demodulated, and rendered for display on any graphics device. The J300 provides hardware video compression and decompression to accelerate applications such as videoconferencing. The J300 supports analog broadcast video output from either compressed or uncompressed video files. Audio support includes a general-purpose, digital signal processor (DSP) to assist in the real-time management of the audio streams and for advanced processing, such as compression, decompression, and echo cancellation. Audio input and output capabilities include stereo analog I/O, digital audio I/O, and a headphone/microphone jack. Analog audio can be digitized to 16 bits per sample at a rate of up to 48 kilohertz (kHz). This paper begins with an overview of some terminology commonly used in the field of broadcast video. The paper then presents the evolution and design of the J300 architecture, including several key enabling The J300 family of video and audio adapters provides a feature-rich set of hardware options for Alpha-based workstations. Unlike earlier attempts to integrate full-motion digital video with general-purpose computer systems, the architecture and design of J300 adapters exploit fast system and I/O buses to allow video data to be treated like any other data type used by the system, independent of the graphics subsystem. This paper describes the architecture used in J300 products, the video and audio features supported, and some key aspects of the hardware design. In particular, the paper describes a simple yet versatile color-map-friendly rendering system that generates high-quality 8-bit image data.
Paediatric nurses' knowledge and practice of mixing medication into foodstuff (2011)

Co-mixing of medication into foodstuff is a common practice. The majority of nurses are unaware of potential drug stability/degradation issues and/or the clinical impact of these practices.
Geometric partitioning made easier, even in parallel (1993)

We present a simple approach for constructing geometric partitions in a way that is easy to apply to new problems. We avoid the use of VC-dimension arguments, and, instead, base our arguments on a notion we call the scaffold dimension, which subsumes the VC-dimension and is simpler to apply. We show how to easily construct (1/r)-nets and (1/r)-approximations for range spaces with bounded scaffold dimension, which immediately implies simple algorithms for constructing (1/r)-cuttings (by straight-forward recursive subdivision methods). More significant than simply being a conceptual simplification of previous approaches, however, is that our methods lead to asymptotically faster and more-efficient EREW PRAM parallel algorithms for a number of computational geometry problems, including the development of the first optimal-work NC algorithm for the well-known 3-dimensional convex hull problem, which solves an open problem of Amato and Preparata. Interestingly, our approach also yields a faster sequential algorithm for the distance selection problem, by the parametric searching paradigm, which solves an open problem posed by Agarwal, Aronov, Sharir, and Suri, and reiterated by Dickerson and Drysdale.
The rate loss of single letter characterization for the &#x201C;dirty&#x201D; multiple access channel (2008)

For general memoryless systems, the typical information theoretic solution, when exists, has a ldquosingle-letterrdquo form. This reflects the fact that optimum performance can be approached by a random code (or a random binning scheme), generated using independent and identically distributed copies of some single-letter distribution. Is that the form of the solution of any (information theoretic) problem? In fact, some counter examples are known, perhaps the most famous being the Korner-Marton ldquotwo help onerdquo problem, where the modulo-two sum of two binary sources is to be decoded from their independent encodings. In this paper we provide another counter example, the ldquodoubly-dirtyrdquo multiple access channel (MAC). Like the Korner-Marton problem, this example is associated with a multiterminal scenario where side information is distributed among several terminals; each transmitter knows part of the channel interference but the receiver is not aware of any part of it. We give an explicit solution for the capacity region of a binary version of the doubly-dirty MAC, demonstrate how this capacity region can be approached using a linear coding scheme, and prove that the ldquobest known single-letter regionrdquo is strictly contained in it. We also state a conjecture regarding a similar rate loss of single letter characterization in the Gaussian case.
Isolation and structural elucidation of new 18-membered macrolide antibiotics, viranamycins A and B. (1991)

Two cytotoxic antibiotics, designated viranamycins A and B, were isolated from the culture broth of Streptomyces sp. CH41. Their structures were elucidated as new 18-membered macrolides related to virustomycin A and concanamycin A from NMR spectral analysis. Viranamycins A and B inhibited the growth of P388 mouse leukemia and KB human squamous-cell-carcinoma cells.
TREPHINE BIOPSY OF BONE WITH SPECIAL REFERENCE TO THE LUMBAR VERTEBRAL BODIES (1949)

The relative inaccessibility of the vertebral body for surgical biopsy makes trephine biopsy valuable in this region. By use of the needle described, excellent, adequate undistorted cores of tissue may be obtained, to aid in the differential diagnosis of many lesions.
Methods for an International Randomized Clinical Trial to Investigate the Effect of Gsk249320 on Motor Cortex Neurophysiology using Transcranial Magnetic Stimulation in Survivors of Stroke (2014)

Although TMS methodological approaches vary, a protocol with standardized procedures was successfully developed and implemented. Using this protocol, centers were formally certified to perform TMS-based neurophysiological measures in this clinical trial of stroke patients. The methodology described is potentially valuable to investigators who might construct future multi-site clinical trials using TMS.
Diminishing Apoptosis by Deletion of Bax or Overexpression of Bcl-2 Does Not Protect against Infectious Prion Toxicity<i>In Vivo</i> (2007)

B-cell lymphoma protein 2 (Bcl-2) and Bcl-2-associated X protein (Bax), key antiapoptotic and proapoptotic proteins, respectively, have important roles in acute and chronic models of neurologic disease. Several studies have implicated Bax and Bcl-2 in mediating neurotoxicity in prion diseases. To determine whether diminishing apoptotic cell death is protective in an infectious prion disease model we inoculated mice that either were null for proapoptotic Bax or overexpressed antiapoptotic Bcl-2. Interestingly, genetic manipulation of apoptosis did not lessen the clinical severity of disease. Moreover, some disease parameters, such as behavioral alterations and death, occurred slightly earlier in mice that are null for Bax or overexpress Bcl-2. These results suggest that Bax and Bcl-2 mediated apoptotic pathways are not the major contributing factor to the clinical or pathological features of infectious prion disease.
An Estimation of Performances of Unidigit and Multidigit Communication Systems (1969)

Some new unidigit, viz. SQ-PCM, HU-PCM, and multidigit, viz. Δ-ΔM, Feedback-PCM and Δ-PCM, PCM systems have been developed and their performances evaluated and compared with those of other similar systems, viz. Δ-M, conventional PCM and D-PCM. It is shown that among all the unidigit systems, HU-PCM has the best coding characteristics and of the multi-digit systems, Δ-ΔM is the best. For RF transmission, PSK-mode has the greatest communication efficiency. HU-PCM is superior to conventional PCM up to a transmission rate of 60 Kbits/sec. and the Δ-ΔM, with a closed loop compandor at a transmission rate of 60 Kbits/sec. is equivalent to a 9-digit PCM system. In an n-digit ΔM, the rate of decrease of the quantizing noise is exponential as in the conventional PCM, moreover the optimum SNR's in n-ΔM are much better than in any other PCM system.
Traditional Resuscitative Practices Fail to Resolve Metabolic Acidosis in Morbidly Obese Patients After Severe Blunt Trauma (2010)

Morbidly obese trauma patients show prolonged metabolic acidosis despite receiving similar volumes and attaining similar end points of resuscitation when compared with patients in other BMI groups. Inadequate resuscitation based on inaccurate end points and metabolic disturbances associated with increased BMI are likely responsible; identification of the etiology, sources, and consequences of this acidosis may provide further insight into the susceptibility of the morbidly obese patient to develop postinjury organ failure.
[Insulin receptor Arg1131-->Gln: a novel mutation in the catalytic loop of insulin receptor observed in insulin resistant diabetes]. (1992)

A novel mutation Arg1131-->Gln in the catalytic loop of insulin receptor (IR) associated with insulin resistant diabetes was detected. A 56-year-old male with hyperinsulinemia (fasting IRI 92 microU/ml) showed moderate impairment in glucose tolerance (HbAlc 7.0%, fructosamine 258 mumol/l, fasting glucose 119 mg/dl, maximum value of blood glucose during 75 g OGTT 220 mg/dl). While insulin binding to erythrocytes IR was normal, the insulin-induced autophosphorylation of the patient's erythrocytes IR in vivo showed marked decrease, suggesting this patient had some defect in the kinase domain (exon 17-21) of IR. PCR-SSCP analysis of kinase domain with a genomic DNA obtained from the patient's leucocytes indicated the presence of some mutations in exon 19. Sequencing analysis in M13 revealed a heterozygous mutation at a position 1131 (CGG-->CAG) substituting Gln for Arg. Four people of patient's family analyzed are revealed to have an identical missense mutation at the same position with the patient.
Chemical constitutents of chamaelirium luteum (2002)

CASE DESCRIPTION-Two adult male castrated cats were evaluated because of a history of constipation, tenesmus, or intermittent vomiting. CLINICAL FINDINGS-Radiography and ultrasonography revealed luminal narrowing in the colon of 1 cat and a colonic mass in the other. A histopathologic diagnosis of colonic adenocarcinoma was made in both cats. TREATMENT AND OUTCOME-Under fluoroscopic guidance, a self-expanding metallic stent was advanced over a wire and across the area of colonic stenosis and deployed. One cat had progressive weight loss but maintained a normal appetite, energy, and a high quality of life. Fecal continence was maintained, and tenesmus was rarely observed. The cat was euthanized because of tumor metastasis 274 days after the colonic stent was placed. The other cat retained fecal continence, and the owners reported subjective improvement in the severity of tenesmus, compared with that prior to stent placement. The cat was euthanized 19 days after stent placement because of perceived decreased quality of life. CLINICAL RELEVANCE-The use of self-expanding metallic stents for alleviation of colonic obstruction secondary to adenocarcinoma in cats appears to be effective. This technique provides a simple, quick, nonsurgical option for palliation in cats with advanced metastatic or systemic disease in which surgical resection may not be possible or warranted.
New phase of superconducting NbN stabilized by heteroepitaxial film growth (1995)

Thin films of a new, metastable phase of superconducting niobium nitride have been grown using pulsed laser deposition (PLD). This new NbN phase is stabilized by heteroepitaxial growth on (100) MgO and is shown to be a primitive cubic (Pm3m) distortion from the typical B1, or rocksalt structure. Structural and electrical characterization reveals that this NbN phase has a higher-superconducting critical temperature and a larger lattice parameter when compared with films of B1-NbN. Growth of this new phase demonstrates that PLD can be used as a synthesis tool to deposit new, metastable materials.
Slow Polymerization of <i>Mycobacterium tuberculosis</i> FtsZ (2000)

The essential cell division protein, FtsZ, from Mycobacterium tuberculosis has been expressed in Escherichia coli and purified. The recombinant protein has GTPase activity typical of tubulin and other FtsZs. FtsZ polymerization was studied using 90 degrees light scattering. The mycobacterial protein reaches maximum polymerization much more slowly ( approximately 10 min) than E. coli FtsZ. Depolymerization also occurs slowly, taking 1 h or longer under most conditions. Polymerization requires both Mg(2+) and GTP. The minimum concentration of FtsZ needed for polymerization is 3 microM. Electron microscopy shows that polymerized M. tuberculosis FtsZ consists of strands that associate to form ordered aggregates of parallel protofilaments. Ethyl 6-amino-2, 3-dihydro-4-phenyl-1H-pyrido[4,3-b][1,4]diazepin-8-ylcarbamate+ ++ (SRI 7614), an inhibitor of tubulin polymerization synthesized at Southern Research Institute, inhibits M. tuberculosis FtsZ polymerization, inhibits GTP hydrolysis, and reduces the number and sizes of FtsZ polymers.