gebhart/patrepeval huggingface dataset:
Effect of peroxynitrite scavenger on brain microvascular nitrotyrosination and S‐nitrosylation (2020)

Objective Peroxynitrite, generated from nitric oxide and superoxide, plays a pathological role in both ischemic and neurodegenerative brain injury. Free radical mediated microvascular dysfunction is a key initiator of cerebrovascular and neurological pathologies. Thus, understanding the acute effects of peroxynitrite on brain microvessels will help identify potential therapeutic targets. The objective of the study was to investigate the impact of peroxynitrite scavenger on the nitrotyrosination and S‐nitrosylation of brain microvessels. Methods Brain microvessels were isolated from the brains of 12‐week‐old female C57bl6 (WT, n= 4) mice by a combination of filtration (40μm – 300μm) and gradient centrifugation. Microvessels were treated with 2.5 μmol/L of peroxynitrite scavenger (FeTMPyP, Fe (III)tetrakis (1‐methyl‐4‐pyridyl) porphyrin pentachlorideporphyrin pentachloride) for 60 minutes at 37°C. Subsequently, western Blots were then performed to detect the nitrotyrosine residues in the protein lysates from microvessels. In addition, we determined the protein S‐nitrosylation in lysates by biotin‐switch method followed by immunoblotting. Nitrotyrosine residue is an indicator of peroxynitrite generation and protein‐S‐nitrosylation is an indirect measure for nitric oxide production. Results Acute treatment with FeTMPyP decreased nitrotyrosination of proteins from microvessels (3.16±0.63 vs 19.79±10.82, p=0.03, n=4 mice) in WT mice. In addition, 2.5 μM of FeTMPyP treatment decreased the protein S‐nitrosylation by 40% (p≤0.05). Conclusions Brain microvessels form peroxynitrite at basal levels. Surprisingly, peroxynitrite inhibition decreases protein S‐nitrosylation in the brain microvessels. Support or Funding Information Support: Undergraduate Summer Research Fellowship by American Physiological Society (Albuck); and National Institute of Health: National Institute of General Medical Sciences and National Institute of Neurological Disorders and Stroke (Katakam: R01NS094834) and National Institute on Aging (Mostany: AG047296).
Correction to “Dynamics of Capillary-Driven Flow in 3D Printed Open Microchannels” (2017)

ADVERTISEMENT RETURN TO ISSUEPREVAddition/CorrectionNEXTORIGINAL ARTICLEThis notice is a correctionCorrection to "Dynamics of Capillary-Driven Flow in 3D Printed Open Microchannels"Robert K. Lade, Jr., Erik J. Hippchen, Christopher W. Macosko, and Lorraine F. Francis*Cite this: Langmuir 2017, 33, 34, 8591Publication Date (Web):August 18, 2017Publication History Published online18 August 2017Published inissue 29 August 2017https://pubs.acs.org/doi/10.1021/acs.langmuir.7b02527https://doi.org/10.1021/acs.langmuir.7b02527correctionACS PublicationsCopyright © 2017 American Chemical Society. This publication is available under these Terms of Use. Request reuse permissions This publication is free to access through this site. Learn MoreArticle Views591Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail PDF (125 KB) Get e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Interim results of a multicenter phase II trial of <i>nab</i>-paclitaxel (<i>nab</i>-P) plus gemcitabine (G) for patients (Pts) with locally advanced pancreatic cancer (LAPC). (2017)

358 Background: Treatment options for pts with LAPC are limited and generally similar to those for metastatic PC (mPC). The phase 3 MPACT trial of pts with mPC demonstrated a > 3-fold shrinkage of primary tumors with nab-P + G vs G, suggesting the potential of nab-P + G for LAPC treatment. Here, we present interim results on disease control rate (DCR), adverse events (AEs), and quality of life (QoL) from the international phase 2 LAPACT trial. Methods: Pts with treatment-naive unresectable LAPC and ECOG performance status of 0 or 1 received 6 cycles (C) of nab-P 125 mg/m 2 + G 1000 mg/m 2 on days 1, 8, and 15 of each 28-day C. After the initial nab-P + G treatment phase, pts without PD and unacceptable AEs were eligible for investigator’s choice (IC) of continued treatment with nab-P + G, chemoradiation, or surgery. Surgery could occur prior to completing 6 C in the case of a major response. Pt-reported QoL was assessed via EORTC QLQ-C30 and QLQ-PAN26 questionnaires at screening and prior to infusion on day 1 of each C. Results: As of Aug 17, 2016, 47 pts completed (28/47, 60%) or discontinued (19/47, 40%) the initial nab-P + G treatment (median, 5 C). Median age was 66 years (range, 44 - 86). The most frequent reasons for discontinuation were AE (10/47 [21%], with the most common being neutropenia and abnormal liver function [2 pts each]) and PD (3/47, 6%). The most common grade ≥ 3 AEs were neutropenia (34%) and anemia (11%). The DCR ≥ 16 weeks was 76% (34/45 efficacy-evaluable pts [defined as having evaluable baseline and ≥ 1 postbaseline scan]; PR, n = 13; SD, n = 21). Twenty-two pts (47%) were assigned by the investigators to an IC treatment: 4 (9%) to continue nab-P + G, 8 (17%) to chemoradiation, and 10 (21%) to surgical resection. Mean QoL scores remained stable during the study, with improved symptom scores for appetite and pain. During the initial nab-P + G treatment phase, most patients reported a complete resolution of certain limitations, including depression (≈ 80%), constipation (≈ 62%), and nausea (≈ 93%). Conclusions: These interim results suggest that for pts with LAPC, nab-P + G is tolerable and produces a promising DCR. On average, QoL scores remained stable during nab-P + G treatments. Clinical trial information: NCT02301143.
Clean-Contaminated Neck Surgery (2006)

The risk of surgical wound infection is correlated with a higher tumor stage and lymph node metastases; it is not associated with the extent of surgery or other factors considered.
Best Practices for Effective Wellbore Cleanup and Displacements in Open-Hole Sand Control Completions (2017)

Abstract At some stage after drilling to target depth and before pumping the gravel packing treatment or before putting the well on production, the drilling fluid is typically displaced from the wellbore. Practices in the industry vary significantly depending on the primary drivers of the completion engineers, sometimes with disastrous results. Inefficient wellbore displacements can cause a variety of problems including increased non-productive time, reduced well productivity and incomplete gravel packing through various mechanisms. In this paper, we detail our best practices to ensure efficient wellbore displacements for sand control completions. In the design phase, these involve various compatibility tests some of which are not commonly done and/or potential problems cannot easily be identified when they are done using conventional test procedures, modeling of fluid-fluid displacements and determining the fluid properties, pump rates and fluid volumes required for effective displacements in a given wellbore geometry and flow paths. On the rig site, they involve several quality control tests, some of which have not previously been implemented.
Ética, ciencia y compromiso político (2017)

Una version previa de este trabajo fue presentada en el II Congreso Argentino de Estudios Sociales en Ciencia y Tecnologia, realizado en la Universidad Nacional de Rio Negro, Sede Andina, San Carlos de Bariloche, del 30 de noviembre al 2 de diciembre de 2016, en la mesa 5.1: Conocimiento, ideologia, hegemonia y capitalismo I, bajo el titulo “A proposito del cientifico politizado”.
Cost-effective On-board Optical Interconnection using Waveguide Sheet with Flexible Printed Circuit Optical Engine (2011)

We have developed a cost-effective on-board optical interconnection sheet using a polymer waveguide with flexible printed circuit optical engines. Error-free operation up to 14 Gb/s was demonstrated using a 12-ch optical interconnection sheet.
Promoting effects of thoria on the nickel-manganese mixed oxide catalysts for the aerobic oxidation of benzyl alcohol (2017)

Due to the recent advancement in the development of various characterization techniques, mixed metal oxide (MMO) based catalysts have gained tremendous attention in the field of catalysis. In this study, we demonstrated the synthesis of a series of novel MMO based catalysts by a facile co-precipitation method. The detailed structure and composition of thoria promoted NiMnO catalysts was investigated using various microscopic and spectroscopic techniques such as, SEM, EDAX, XRD, TGA, BET and TPR. In order to study the effect of the content of thorium oxide on the catalytic activity of the as-prepared material various samples were prepared by the addition of low quantities of thorium oxide with 1%, 3% and 5% on NiMnO. The catalytic performances of the as-prepared catalysts were evaluated towards the aerobic oxidation of benzyl alcohol using molecular oxygen as oxidant. Furthermore, in order to investigate the effect of the calcination temperatures on the catalytic activities of the as-prepared materials, the samples were calcined at three different temperatures at 300 °C, 400 °C and 500 °C. The catalysts displayed significant enhancement in catalytic activity towards the catalytic conversion of benzyl alcohol (C6H5CH2OH) to benzaldehyde (C6H5CHO). Detailed kinetic studies of the reactions using gas chromatography have revealed that the variation of calcination temperature and the percentage of thoria had significant effect on the catalytic performances of the materials. Among all synthesized catalysts ThO2-(5%)-NiMnO catalyst calcined at 400 °C exhibited the highest catalytic performance and stability for the selective oxidation of alcohols.
Properties of the protein kinase C-phorbol ester interaction (1989)

The properties of the protein kinase C (PKC)-phorbol ester interaction were highly dependent on assay methods and conditions. Binding to cation-exchange materials or adsorption to gel matrices resulted in PKC that was capable of binding phorbol 12,13-dibutyrate (PDBu). The extraneous interactions were eliminated by measuring phorbol ester binding with a gel filtration chromatography assay in the presence of bovine serum albumin (BSA). In the absence of calcium, free PKC did not bind PDBu or phospholipids. Calcium caused structural changes in PKC which enhanced its interaction with surfaces such as the gel chromatography matrix. While BSA prevented this interaction, it did not interfere with PKC association with acidic phospholipids. Interaction of PKC with phospholipid resulted in two forms of membrane-associated PKC. The initial calcium-dependent and reversible form of membrane-associated PKC was capable of binding PDBu. Both PKC and PDBu were released from this complex by calcium chelation. Sustained interaction with phospholipid vesicles resulted in a PKC-membrane complex that could not be dissociated by calcium chelation and appeared to result from insertion of PKC into the hydrocarbon portion of the phospholipid bilayer. Membrane insertion was observed at calcium concentrations of 2-500 microM and with membrane compositions of 10-50% acidic phospholipid. However, the extent of insertion was dependent on the binding conditions and was promoted by high phospholipid to PKC ratios, high calcium, the presence of phorbol esters, high membrane charge, and long incubations.(ABSTRACT TRUNCATED AT 250 WORDS)
Transforming Growth Factor-β1 Downregulates Vascular Endothelial Growth Factor-D Expression in Human Lung Fibroblasts via the Jun NH2-Terminal Kinase Signaling Pathway (2014)

Vascular endothelial growth factor (VEGF)-D, a member of the VEGF family, induces both angiogenesis and lymphangiogenesis by activating VEGF receptor-2 (VEGFR-2) and VEGFR-3 on the surface of endothelial cells. Transforming growth factor (TGF)-β1 has been shown to stimulate VEGF-A expression in human lung fibroblast via the Smad3 signaling pathway and to induce VEGF-C in human proximal tubular epithelial cells. However, the effects of TGF-β1 on VEGF-D regulation are unknown. To investigate the regulation of VEGF-D, human lung fibroblasts were studied under pro-fibrotic conditions in vitro and in idiopathic pulmonary fibrosis (IPF) lung tissue. We demonstrate that TGF-β1 downregulates VEGF-D expression in a dose- and time-dependent manner in human lung fibroblasts. This TGF-β1 effect can be abolished by inhibitors of TGF-β type I receptor kinase and Jun NH2-terminal kinase (JNK), but not by Smad3 knockdown. In addition, VEGF-D knockdown in human lung fibroblasts induces G1/S transition and promotes cell proliferation. Importantly, VEGF-D protein expression is decreased in lung homogenates from IPF patients compared with control lung. In IPF lung sections, fibroblastic foci show very weak VEGF-D immunoreactivity, whereas VEGF-D is abundantly expressed within alveolar interstitial cells in control lung. Taken together, our data identify a novel mechanism for downstream signal transduction induced by TGF-β1 in lung fibroblasts, through which they may mediate tissue remodeling in IPF.
Cell-cycle dysregulation and the molecular mechanisms of prostate cancer (2000)

Prostate cancer is the most common cause of non-cutaneous cancer in men and although frequently latent is the second commonest cause of death. Screening for the disease was historically based on symptoms of urethral obstruction, clinical examination of the prostate gland and serum measurements of prostate specific antigen. As prostate cancer growth in the early stages is enhanced by androgens, the mainstay of therapy has been androgen ablation by pharmaco-therapeutic or surgical means. The subsequent development of androgen therapy resistant prostate cancer in many patients, for whom therapeutic options remain limited, has led researchers to focus attention on understanding the molecular genetics of prostate cancer. The array of genetic abnormalities observed in prostate tumors, which include changes in components of the cell cycle, suggest the disease is quite heterogeneous and may require further sub-classification based on genetic markers. Such analyses may lead to identification of relevant new prognostic and therapeutic indicators. The advent of transgenic mouse models of prostate cancer may provide a critical tool for the implementation of rational genetic based therapeutics and alternate drug design.
The Reaction Rates of O<sub>2</sub> with Closed-Shell and Open-Shell Al<sub><i>x</i></sub><sup>–</sup> and Ga<sub><i>x</i></sub><sup>–</sup> Clusters under Single-Collision Conditions: Experimental and Theoretical Investigations toward a Generally Valid Model for the Hindered Reactions of O<sub>2</sub> with Metal Atom Clusters (2014)

In order to characterize the oxidation of metallic surfaces, the reactions of O2 with a number of Al(x)(-) and, for the first time, Ga(x)(-) clusters as molecular models have been investigated, and the results are presented here for x = 9-14. The rate coefficients were determined with FT-ICR mass spectrometry under single-collision conditions at O2 pressures of ~10(-8) mbar. In this way, the qualitatively known differences in the reactivities of the even- and odd-numbered clusters toward O2 could be quantified experimentally. To obtain information about the elementary steps, we additionally performed density functional theory calculations. The results show that for both even- and odd-numbered clusters the formation of the most stable dioxide species, [M(x)O2](-), proceeds via the less stable peroxo species, [M(x)(+)···O2(2-)](-), which contains M-O-O-M moieties. We conclude that the formation of these peroxo intermediates may be a reason for the decreased reactivity of the metal clusters toward O2. This could be one of the main reasons why O2 reactions with metal surfaces proceed more slowly than Cl2 reactions with such surfaces, even though O2 reactions with both Al metal and Al clusters are more exothermic than are reactions of Cl2 with them. Furthermore, our results indicate that the spin-forbidden reactions of (3)O2 with closed-shell clusters and the spin-allowed reactions with open-shell clusters to give singlet [M(x)(+)···O2(2-)](-) are the root cause for the observed even/odd differences in reactivity.
CD4 TCRBV CDR3 Analysis in prevalent SLE Cases from two ethnic Groups (1999)

We examined CD4+ T cell TCRBV-CDR3 transcripts from 19 lupus patients and 16 controls to test the hypothesis that CD4+ TCRBV-CDR3 expression in SLE differs from normals. Within the disease group we also performed exploratory analyses to determine the association between risk of oligoclonality and HLA-DRB specificities and the duration of the CDR3 patterns. Oligoclonal patterns consistent with CDR3 restriction were three times more likely in SLE than in controls (OR = 3.7). TCRBV1, BV4, BV5.1, BV7, BV9, BV18 and BV22 gene segment CDR3 patterns of oligoclonality were seen exclusively among lupus patients. HLA-DRB3 increased the risk of oligoclonal expression in SLE. In four patients studied over time, the pattern of TCRBV-CDR3 expression was stable in a second sample obtained 6-14 months later. The increased frequency of CD4+ T cell TCRBV-CDR3 oligoclonal expression in SLE when compared to controls and the persistence of these patterns are consistent with an expanded pool of autoreactive CD4 T cells in SLE which recognize peptides derived from autoantigens. The association of HLA-DRB3 genes with increased risk of CDR3 oligoclonality among the SLE subjects is compatible with the hypothesis that molecules encoded by HLA-DRB3 may facilitate autoantigen recognition by CD4 T cells.
Robotic execution of a surgical plan (2003)

The ROBODOC Surgical Assistant was developed to eliminate some common problems associated with total hip replacement surgery: improper fit and placement of the prosthetic implant due to imprecise preparation of the femoral canal. The system combines two separate technologies: CAD/CAM (computer-aided design/computer-aided manufacturing) for preoperative planning and robotics for surgical preparation of a cavity in the femur. The ROBODOC's preoperative planning system blends 3-D imaging technology with a user interface designed for novice computer users. In the operating room, the robot executes the surgeon's plan for femoral canal preparation, replacing mallet-driven broaches and other hand-held tools. The most outstanding feature of the integrated system is the tight link between preoperative planning and surgical execution: no longer will surgeons rely on hand-eye coordination and surgical 'feel' to execute a plan that consists of a 2-D blueprint for a complex operation.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
A word from the new Editorial Board Chair – Professor Philip Gale, University of Southampton, UK (2013)

A graphical abstract is available for this content
Controlled electron acceleration from dielectric nanospheres in intense few-cycle laser fields (2011)

We demonstrate the emission and directional control of highly energetic electrons from isolated SiO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> nanoparticles in few-cycle laser fields with well defined waveform at intensities close to the tunneling regime.
Autonomous Robot for Monitoring of Outdoor Field Health (2020)

This paper presents the design and development of an autonomous intelligent robot on the Robotic Operating System (ROS) with automated navigation to monitor the grass health in turfs by spraying fertilizers or nutrients for an early detection of stress in the leaves through chlorophyll index estimation and further the disease detection is performed by processing the images snapped by the bot. The accuracy in regards to automated navigation of the bot is in the order of twenty centimeters in outdoor operations, while the accuracy of stress detection in leaves has been about 90% on field trials, which makes the system very promising for a commercial deployment.
Re: Presence of Tumor Necrosis is Not a Significant Predictor of Survival in Clear Cell Renal Cell Carcinoma: Higher Prognostic Accuracy of Extent Based Rather Than Presence/Absence Classification (2009)

No AccessJournal of UrologyLetter to the Editor/Errata1 Dec 2009Re: Presence of Tumor Necrosis is Not a Significant Predictor of Survival in Clear Cell Renal Cell Carcinoma: Higher Prognostic Accuracy of Extent Based Rather Than Presence/Absence ClassificationT. Klatte, J. W. Said, M. de Martino, J. Larochelle, B. Shuch, J. Y. Rao, G. V. Thomas, F. F. Kabbinavar, A. S. Belldegrun and A. J. Pantuck J Urol 2009; 181: 1558–1564 Rodney H. Breau, John C. Cheville, Christine M. Lohse, Eugene D. Kwon, and Michael L. Blute Rodney H. BreauRodney H. Breau More articles by this author , John C. ChevilleJohn C. Cheville More articles by this author , Christine M. LohseChristine M. Lohse More articles by this author , Eugene D. KwonEugene D. Kwon More articles by this author , and Michael L. BluteMichael L. Blute More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2009.08.066AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail References 1 : Inadequate statistical power of negative clinical trials in urological literature. J Urol2006; 176: 263. Link, Google Scholar 2 : An outcome prediction model for patients with clear cell renal cell carcinoma treated with radical nephrectomy based on tumor stage, size, grade and necrosis: the SSIGN score. J Urol2002; 168: 2395. Link, Google Scholar 3 : Histologic coagulative tumor necrosis as a prognostic indicator of renal cell carcinoma aggressiveness. Cancer2005; 104: 511. Google Scholar 4 : External validation of the Mayo Clinic stage, size, grade, and necrosis (SSIGN) score for clear-cell renal cell carcinoma in a single European centre applying routine pathology. Eur Urol2008; . Epub ahead of print.. Google Scholar 5 : The ‘stage, size, grade and necrosis' score is more accurate than the University of California Los Angeles Integrated Staging System for predicting cancer-specific survival in patients with clear cell renal cell carcinoma. BJU Int2009; 103: 165. Google Scholar 6 : External validation of the Mayo Clinic cancer specific survival score in a Japanese series of clear cell renal cell carcinoma. J Urol2008; 180: 1290. Link, Google Scholar 7 : Postoperative surveillance for renal cell carcinoma: a multifactorial histological subtype specific protocol. BJU Int2009; 104: 778. Google Scholar Departments of Urology, and Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota© 2009 by American Urological AssociationFiguresReferencesRelatedDetails Volume 182Issue 6December 2009Page: 2979-2981 Advertisement Copyright & Permissions© 2009 by American Urological AssociationMetricsAuthor Information Rodney H. Breau More articles by this author John C. Cheville More articles by this author Christine M. Lohse More articles by this author Eugene D. Kwon More articles by this author Michael L. Blute More articles by this author Expand All Advertisement PDF DownloadLoading ...