gebhart/patrepeval huggingface dataset:
Effects of Trehalose and Sucrose on Gene Expression Related to Senescence of Cut Astilbe (<i>Astilbe</i> × <i>arendsii</i> Arends) Flowers (2020)

Cut astilbe (Astilbe × arendsii) inflorescences have many small florets with low ethylene sensitivity, and a short vase life. The senescence of flowers is complex and probably regulated by programmed cell death, but specific senescence-related genes have not been widely studied, apart from ethylene-sensitive species. In this study, we investigated the effects of continuous treatments with 4% sucrose (Suc), 2% trehalose (Tre), and a combination (Suc+Tre) on postharvest quality and senescence-related gene expression in cut flowers of the astilbe ‘Gloria Purpurea’. Vase life was extended from 3.7 days to 10.3, 9.4, and 12.4 days by treatment with Suc, Tre and Suc+Tre, respectively. Florets were sampled at 0 days (0 d) and 2 days after harvest (2 d). De novo RNA-sequencing (RNA-seq) of floret tissues produced 89,705 unique sequences, and 2,517, 979, 609, and 1,846 differentially expressed genes were identified from the 0d_Control (Cont) vs. 2d_Cont, 2d_Cont vs. 2d_Suc, 2d_Cont vs. 2d_Tre, and 2d_Cont vs. 2d_Suc+Tre libraries, respectively. Gene Ontology (GO) analysis indicated that expression of many photosynthesis-related genes was upregulated in 2d_Cont florets, suggesting the photosynthesis system is activated in senescing florets. Kyoto Encyclopedia of Genes and Genomes analysis showed that WRKY22, related to leaf senescence, was more highly upregulated in 2d_Cont compared with 0d_Cont, 2d_Suc, and 2d_Tre in the Mitogen-activated Protein Kinase (MAPK) signaling pathway. Using Fragments Per Kilobase per Million reads (FPKM) values from RNA-seq, candidate genes associated with senescence including the no apical meristem, ATAF1/2 and cup-shaped cotyledon transcription factor 29 (NAC029), senescence-associated gene 12 (SAG12), and peroxidase 21 (PER21) were selected. Suc+Tre in particular, the most effective treatment for prolonging vase life, downregulated NAC029, SAG12, and PER21 expression at 2 d. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) validated the RNA-seq results, i.e., similar expression trends were found in the three genes, although relatively weak expression levels were detected compared with FPKM values from RNA-seq. The effect of Tre alone on the three genes’ expression levels was relatively small compared with treatments containing Suc, suggesting that Tre may delay floret senescence by altering genes involved in water transport such as Aquaporin tonoplast intrinsic protein 1-3. This study revealed several candidate genes and GO terms involved in the senescence of cut florets, but further study is needed, especially on key genes including NAC029 and WRKY22.
Role of Gastroesophageal Reflux Disease in Patients with Cervical Symptoms (1995)

Otolaryngologists commonly see patients with various nonspecific upper aerodigestive or "cervical" symptoms. The relationship between gastroesophageal reflux disease and these symptoms has been studied but remains unclear. We reviewed the records of 216 patients with various cervical symptoms. Each patient underwent a uniform investigation that included barium swallow, esophageal manometry, acid stimulation testing, and esophagoscopy with distal biopsy. Patients were treated with a combination of a histamine H2 blocker or omeprazole and a prokinetic agent. Follow-up was obtained in 194 patients. Overall, evidence of gastroesophageal reflux disease was detected in 73% of patients. Complete resolution or improvement of symptoms was seen in 84% of patients with treatment. We believe gastroesophageal reflux disease is an important factor in the cause of cervical problems.
Improving formal timing analysis of switched ethernet by exploiting FIFO scheduling (2015)

Ethernet is an emerging technology in the automotive domain and is capable to overcome the bandwidth and scalability limits of traditional buses like CAN or FlexRay. Formal performance analysis methods are required to verify the timing, e.g. by providing upper bounds on end-to-end latencies, in safety-critical real-time systems, such as automotive control and advanced driver assistance systems. In many real-time capable Ethernet implementations such as IEEE 802.1Q or AVB, frames can be prioritized and frames of equal priority are scheduled in FIFO order at the switch ouput ports. In this paper, we show how to exploit Ethernet's FIFO scheduling in a compositional formal performance analysis to derive tighter timing guarantees. In an automotive Ethernet setup, our proposed analysis leads to a significant reduction in end-to-end latency guarantees.
A method for the extraction of phonetically-rich triphone sentences (2014)

A method is proposed for compiling a corpus of phonetically-rich triphone sentences; i.e., sentences with a high variety of triphones, distributed in a uniform fashion. Such a corpus is of interest for a wide range of contexts, from automatic speech recognition to speech therapy. We evaluated this method by building phonetically-rich corpora for Brazilian Portuguese. The data employed comes from Wikipedia's dumps, which were converted into plain text, segmented and phonetically transcribed. The method consists of comparing the distance between the triphone distribution of the available sentences to an ideal uniform distribution, with equiprobable triphones. A greedy algorithm was implemented to recognize and evaluate the distance among sentences. A heuristic metric is proposed for pre-selecting sentences for the algorithm, in order to quicken its execution. The results show that, by applying the proposed metric, one can build corpora with more uniform triphone distributions.
Adsorption of Heavy Metal Ions by Immobilized Phytic Acid (1997)

Phytic acid (myoinositol hexaphosphate) or its calcium salt, phytate, is an important plant constituent. It accounts for up to 85% of total phos­phorus in cereals and legumes. Phytic acid has 12 replaceable protons in the phytic molecule, rendering it the ability to complex with multivalent cations and positively charged proteins. Poly 4-vinyl pyridine (PVP) and other strong-based resins have the ability to adsorb phytic acid. PVP has the highest adsorption capacity of 0.51 phytic acid/resins. The PVP resin was used as the support material for the immobilization of phytic acid. The immobilized phytic acid can adsorb heavy metal ions, such as cad­mium, copper, lead, nickel, and zinc ions, from aqueous solutions. Adsorption isotherms of the selected ions by immobilized phytic acid were conducted in packed-bed column at room temperature. Results from the adsorption tests showed 6.6 mg of Cd2+, 7 mg of Cu2+, 7.2 mg of Ni2+, 7.4 mg of Pb2+, and 7.7 mg of Zn2+ can be adsorbed by each gram of PVP-phytic acid complex. The use of immobilized phytic acid has the potential for removing metal ions from industrial or mining waste water.
Reduction by ICRF-187 of Acute Daunorubicin Toxicity in Syrian Golden Hamsters (1983)

No AccessJournal of Urology1 Dec 1983Reduction by ICRF-187 of Acute Daunorubicin Toxicity in Syrian Golden Hamsters E.H. Herman, A.N. El-hage, V.J. Ferrans, and D.T. Witiak E.H. HermanE.H. Herman More articles by this author , A.N. El-hageA.N. El-hage More articles by this author , V.J. FerransV.J. Ferrans More articles by this author , and D.T. WitiakD.T. Witiak More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(17)51772-3AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail "Reduction by ICRF-187 of Acute Daunorubicin Toxicity in Syrian Golden Hamsters." The Journal of Urology, 130(6), p. 1248 © 1983 by The American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 130 Issue 6 December 1983 Page: 1248 Advertisement Copyright & Permissions© 1983 by The American Urological Association Education and Research, Inc.Metrics Author Information E.H. Herman More articles by this author A.N. El-hage More articles by this author V.J. Ferrans More articles by this author D.T. Witiak More articles by this author Expand All Advertisement PDF downloadLoading ...
AdCD40L—Crossing the Valley of Death? (2012)

CD40-mediated cancer therapy has been under development since it became clear that CD40 plays a profound role in the stimulation of adaptive immune responses. Further, CD40 signaling on tumor cells may lead to growth arrest or even apoptosis that improves therapy outcome. The therapeutic window is appealing since the immune system is selective and normal cells do not apoptose upon CD40 signaling. AdCD40L is an adenoviral-based immunostimulatory gene therapy under evaluation for its efficacy to treat cancer. Because of its nature, the adenoviral backbone will stimulate TLRs while CD40L potentiates the shifts toward Th1 type of immunity. AdCD40L has shown efficacy in various murine models, and safety studies have been performed on dog patients and in human clinical trials. AdCD40L has been used for both ex vivo gene modification of tumor cell vaccines as well as for direct intratumoral injections. Lately, an oncolytic vector has been used to further increase the eradication of solid tumors that as a consequence further boosts the release of tumor antigens and creates danger signaling in the tumor micro milieu. This review discusses the currently unfolding mechanisms of action of AdCD40L gene therapy and its possibilities to reach clinical care.
INFLUENCE OF S18327, CLOZAPINE AND OTHER ANTIPSYCHOTICS UPON DIZOLCIPINE (DIZ)-INDUCED SPONTANEOUS TAIL-FLICKS (STFS) IN RATS IN RELATION TO THEIR ACTIONS AT MULTIPLE MONOAMINERGIC RECEPTORS (1999)

s of the First Congress of the Behavioral Pharmacology Society and the European Behavioural Pharmacology Society: PDF Only
Have advanced research technologies made real impact on respiratory medicine? (2010)

Tremendous sophistication in our ability to detect, measure and manipulate the genes and proteins involved in lung disease has provided unique insights to the pathobiology of a number of lung disorders affecting humans today. Gene array analysis and genetic manipulation of animals have helped to identify novel pathways and their potential role in disease initiation and progression. Proteomics is a relatively novel tool in molecular research in respiratory medicine. Silencing gene expression by using small interfering RNA may, sooner than later, become part of novel therapies. Despite the excitement, to date, there has been little direct impact on therapeutic interventions available to clinicians. However, the rapidity with which these early data have accumulated, and the expected advances in bioinformatics and systems biology, should bring forward tangible therapeutic benefits for many acute and chronic lung diseases within the next ten years.
Social and Historical Viewpoint of New Historical Fictions (2000)

Different from America's new historicism used as a method of literary criticism,Chinese new historical fictions, guided by the viewpoint of circulation ,aim at overturning the class viewpoint and social devolopment expressed in the “seventeen year novels”(1949-1966),and reflect the background of postmodernist theory.The fictions of Chinese new historicism do not shake off the pattern in which ideas come first,and also the ideas are contrary to the historical facts,so they are farther away from reality than the “seventeen year novels”
Mechanism of action of streptomycin : studies with polynucleotide phosphorylase and ribosomes (2011)

Some of the previous work on the mechanism of action of streptomycin has indicated the general area of nucleic acid metabolism as being a possible site of action. Specifically, a report has appeared that streptomycin inhibits the "exchange reaction" catalyzed by polynucleotide phosphorylase. Therefore, the possible action of streptomycin on polynucleotide phosphorylase from a streptomycin sensitive strain of Escherichia coli has been reinvestigated. In preparation for the study on polynucleotide phosphorylase, the nucleoside diphosphates of adenosine, guanosine, cytidine, and uridine were synthesized in sufficient yield and of a satisfactory purity by the recently developed method of Moffatt and Khorana. It was not possible to confirm the inhibition of the polynucleotide phosphorylase catalyzed "exchange reaction" reported by Kornberg, using enzyme from our streptomycin sensitive E. coli SA. Nor was it possible to demonstrate any inhibition of the polymerization reaction catalyzed by this enzyme, or any effect on the sedimentation properties of the polymer so formed. However, the diamines putrescine, cadaverine, spermine, and spermidine, while having no effect on the time course of the polymerization reaction, did lower the sedimentation coefficient of the polymer formed and cause a more heterogeneous polymer to be formed. Streptomycin has been suggested as an inhibitor of protein synthesis. Therefore, a study of the possible effects of streptomycin on ribosomes from E. coli was made. These subcellular particles have been shown to be a site of protein synthesis. Dihydrostreptomycin (DHSM) interacted strongly with the ribosomes. It was found that most of the ribonucleo-protein precipitated when ribosomes were dialyzed overnight at 4° against buffer containing about 500 μg./ml. of DHSM. A study of ultracentrifuge patterns of dialysates of ribosomes against lower levels of DHSM indicated that disruption, with a loss of discrete sedimentation coefficients, occurred when the level of DHSM was about 350 μg./ml. A study of elution patterns from DEAE-cellulose columns indicated only a partial change in the pattern after breakdown. Examination of sedimentation coefficients of ribosome dialysates at lower DHSM levels indicated no specific effect of DHSM. The autodegradation of ribosomes by polynucleotide phosphorylase was studied. DHSM, at low levels, had an effect on the time course of the auto-degradation. A study of the distribution of induced β-D-galactosidase associated with the ribosomes indicated that it was associated with that protein of the ribosomes not precipitated by DHSM. It can be concluded that streptomycin interacts strongly with ribosomes. This gives support to recent claims that streptomycin inhibits ‘in vitro' protein synthesis, and that the site of this inhibition is the ribosome.
True amplitude BiSAR image reconstruction via backprojection and image domain scaling (2009)

We consider a bistatic synthetic aperture radar (BiSAR) system operating in non-ideal imaging conditions with receive and transmit antennas traversing arbitrary flight trajectories over a non-flat topography; transmitting arbitrary waveforms along flight trajectories etc. In1 we developed a generalized filtered-backprojection (GFBP) method for BiSAR image formation applicable to such non-ideal imaging scenarios. The method puts edges not only at the right location and orientation, but also at the right strength resulting in true amplitude images. The main computational complexity of the GFBP method comes from the spatially dependent filtering step. In this work, we present an alternative, novel FBP method applicable to non-ideal imaging scenarios resulting in true amplitude images. The method involves ramp filtering in data domain and image domain scaling. Additionally, the method results in fast, computationally efficient implementation than that of GFBP methods.
Demonstration of Entrainment and Presence of Slow Conduction During Ventricular Tachycardia in Arrhythmogenic Right Ventricular Dysplasia (1994)

During VT in two cases with arrhythmogenic right ventricular dysplasia, entrainment criteria, constant fusion beats except for the last entrainment beat, progressive fusion, and a localized conduction block associated with interruption of VT, were demonstrated with rapid ventricular pacing performed during VT. Furthermore, a long conduction interval was present during entrainment from the pacing site to the earliest activation site during VT, indicating the presence of a slow conduction area. VT in these cases was, thus, due to reentry with an area of slow conduction within the circuit.
On k-hulls and related problems (1984)

For any set X of points (in any dimension) and any k = 1,2, ..., we introduce the concept of the k-hull of X. This unifies the well-known notion of 'convex hulls' with the notion of 'centers' recently introduced by F.F. Yao. The concept is intimately related to some other concepts (k-belts, k-sets) studied by Edelsbrunner, Welzl, Lovász, Erdös and others.
CFD evaluation of Mach 17 HYPULSE scramjet combustor data (1991)

Three-dimensional finite rate chemistry solutions are performed on a single fuel injector configuration. The results are compared with limited experimental data obtained from the HYPULSE expansion tube facility at simulated flight Mach 17 flow conditions. All comparisons, except for wall heat flux, were in excellent agreement. Key findings from this study are useful in interpretation of the experimental measurements.
Strawberry-Shaped Skull in Fetal Trisomy 18 (1992)

During an 8-year period, a strawberry-shaped skull (flattening of the occiput with pointing of the frontal bones), was observed in 54 (3%) of the 2,086 fetuses that underwent karyotyping in our unit because of fetal malformations and/or growth retardation. In all 54 cases with a strawberry-shaped skull, there were other fetal malformations; in 43 (80%) fetuses, there was trisomy 18 and in 1 triploidy. Therefore, the ultrasonographic finding of a strawberry-shaped skull should initiate a diligent search for the presence of other markers of trisomy 18 and is a strong indication for fetal karyotyping. However, in the total series of 2,086 fetuses with malformations and/or growth retardation, there were another 40 fetuses with trisomy 18 and 41 with triploidy who did not have a strawberry-shaped skull.
The natural compound forskolin synergizes with dexamethasone to induce cell death in myeloma cells via BIM (2015)

Abstract We have previously demonstrated that activation of the cyclic adenosine monophosphate (cAMP) pathway kills multiple myeloma (MM) cells both in vitro and in vivo . In the present study we have investigated the potential of enhancing the killing of MM cell lines and primary MM cells by combining the cAMP-elevating compound forskolin with the commonly used MM therapeutic drugs melphalan, cyclophosphamide, doxorubicin, bortezomib and dexamethasone. We observed that forskolin potentiated the killing induced by all the tested agents as compared to treatment with the single agents alone. In particular, forskolin had a synergistic effect on the dexamethasone-responsive cell lines H929 and OM-2. By knocking down the proapoptotic BCL-2 family member BIM, we proved this protein to be involved in the synergistic induction of apoptosis by dexamethasone and forskolin. The ability of forskolin to maintain the killing of MM cells even at lower concentrations of the conventional agents suggests that forskolin may be used to diminish treatment-associated side effects. Our findings support a potential role of forskolin in combination with current conventional agents in the treatment of MM.
Caveolin‐1 Scaffolding Domain Regulates Store‐Operated Calcium Influx by Interacting with IP3R and TRPC1 in endothelial cells (2008)

Caveolin‐1 (Cav1) expression is required for store‐operated Ca 2+ entry in endothelial cells. We have shown previously Cav1 scaffolding domain (CSD) peptide interacted with the C‐terminus of TRPC1 and inhibited Ca 2+ influx in endothelial cells ( Mol Pharmacol.70:1174–1183, 2006 ). To understand the basis of Cav1 regulation of Ca 2+ influx, we ectopically expressed CSD truncated Cav1 (Cav1ΔCSD) and TRPC1 C‐terminus truncated (TRPC1‐CΔ781–789) mutants in endothelial cells. We observed >2‐fold increase in thrombin‐induced store Ca 2+ release‐activated Ca 2+ influx in Cav‐1ΔCSD expressing cells compared to controls. In contrast, TRPC1‐CΔ781–789 mutant expression abolished thrombin‐induced Ca 2+ influx. Immunoprecipitation studies revealed that WT‐Cav‐1 and WT‐TRPC1 interacted with endogenous IP 3 R. Whereas expression of TRPC1‐CΔ781–789 with WT‐Cav1 resulted in ∼50% reduction in the ability of IP 3 R binding to TRPC1. Interestingly, expression of Cav1ΔCSD with WT‐TRPC1 caused >80% reduction in the ability of IP 3 R binding to TRPC1. We also assessed the direct binding of IP 3 R with Cav1. Importantly WT‐Cav1 but not the Cav1ΔCSD interacted with endogenous IP 3 R. Co‐expression of TRPC1‐CΔ781–789 and WT‐Cav1 increased Cav1 binding to IP 3 R. These results suggest that Cav1 interacts with IP 3 R and TRPC1 through CSD to form IP 3 R‐Cav1‐TRPC1 complex and thus regulates store‐operated Ca 2+ entry in endothelial cells.
Power spectral density functions for Si wafer surfaces using six measurement techniques (2001)

Power spectral densities (PSDs) were used to characterize a set of surfaces over a wide range of lateral as well as perpendicular dimensions. Twelve 200-mm-diameter Si wafers were prepared and the surface finishes ranged from as-ground wafers to epitaxial wafers. The wafer surfaces were then measured with different methods: atomic force microscopy, angle-resolved light scatter, interferometric microscopy, optical profiling, stylus profiling, and capacitance-based wafer thickness gaging. The data were used to compute one-dimensional PSDs and the curves were plotted as functions of spatial frequencies, comparing results for different samples or for different instruments.
Principles of ACL Revision Surgery and Rehabilitation (2005)

Revision anterior cruciate ligament (ACL) surgery is becoming increasingly common as the number of primary ACL reconstructions continues to rise. Revision ACL surgery is indicated for selected patients with recurrent instability after a failed primary procedure. Rehabilitation for primary ACL reconstructions has undergone an evolution, although very little has been published regarding rehabilitation following revision surgery. It is established that rehabilitation protocols following revision reconstruction should be more conservative than those used for patients with primary ACL reconstructions. The goal of revision surgery and rehabilitation is to recreate a functional knee that is stable for activities of daily living, and perhaps, stable enough to permit a return to sport. Rehabilitation following revision surgery must be individualized depending on a variety of factors including: staging, removal of hardware, graft selection, graft fixation, chondral defects, meniscal pathology, associated instability patterns, and alignment concerns. However, as with primary ACL rehabilitation, in general, the goal of early range of motion is crucial.
Hands-On Pattern Recognition: Challenges in Machine Learning, volume 1 (2011)

Recently organized competitions have been instrumental in pushing the state-of-the-art in machine learning, establishing benchmarks to fairly evaluate methods, and identifying techniques that really work. This volume in the Challenges in Machine Learning series harvests three years of effort of hundreds of researchers who have participated in three competitions organized around five datasets from various application domains, designed to explore issues of data representation, model selection, and performance prediction.
Effect of H-7 on cultured human trabecular meshwork cells. (2001)

H-7 induces alterations in cell shape, actin cytoskeleton, and associated focal adhesions in cultured HTM cells, which may be responsible for the effects of H-7 on outflow facility in live monkey eyes.