gebhart/patrepeval huggingface dataset:
EASI—enrichment of alternatively spliced isoforms (2006)

Alternative splicing produces more than one protein from the majority of genes and the rarer forms can have dominant functions. Instability of alternative transcripts can also hinder the study of regulation of gene expression by alternative splicing. To investigate the true extent of alternative splicing we have developed a simple method of enriching alternatively spliced isoforms (EASI) from PCRs using beads charged with Thermus aquaticus single-stranded DNA-binding protein (T.Aq ssb). This directly purifies the single-stranded regions of heteroduplexes between alternative splices formed in the PCR, enabling direct sequencing of all the rare alternative splice forms of any gene. As a proof of principle the alternative transcripts of three tumour suppressor genes, TP53, MLH1 and MSH2, were isolated from testis cDNA. These contain missing exons, cryptic splice sites or include completely novel exons. EASI beads are stable for months in the fridge and can be easily combined with standard protocols to speed the cloning of novel transcripts.
Efficient variational inference for the dynamic harmonic model (2006)

In this paper, we develop a class of probability models that are potentially useful for various music applications such as polyphonic transcription, source separation, restoration or denoising. This class unifies and extends several models such as sinusoidal and harmonic models, additive synthesis model, Gabor regression and probabilistic phase vocoder. We overcome computational intractability issues by introducing structured variational (mean-field) approximations that lead to efficient local message passing algorithms.
The effects of inspiratory resistive training on respiratory muscle function in patients with muscular dystrophy (1985)

The effects of inspiratory resistive training on respiratory muscle function was evaluated in 11 patients with Duchenne, limb-girdle, and facio-scapulo-humeral (FSH) type muscular dystrophy. Muscle training consisted of breathing against an inspiratory resistance for two 15-minute sessions each day while at home. Following 6 weeks of training, there were significant increases in the maximum resistance that could be tolerated for at least 5 minutes (P < 0.01) and also in the maximum duration that ventilations equal to 30%, 50%, 70%, and 90% of the maximum voluntary ventilation could be sustained (P < 0.05). In six patients who trained for an additional 6-week period, respiratory muscle endurance increased even further. The degree of improvement in respiratory muscle endurance was positively correlated with baseline vital capacity (r = 0.84, P < 0.05) and maximal inspiratory pressure (r = 0.76, P < 0.05). Spirometry, functional residual capacity, and maximal inspiratory and expiratory pressures were not affected by training. We conclude that inspiratory resistive training improves respiratory muscle endurance in muscular dystrophy patients. Improvement in respiratory muscle function may serve to delay the onset of respiratory complications in patients with muscular dystrophy.
A New Approach to Potentiate Site-Specific Hybridization: A set of Hydrophobic Heterobifunctional Short Oligodeoxyribonucleotides (1995)

Abstract An effective approach to enhance the short oligonucleotide reagent to attack ssDNA based on the use of a set of short oligonucleotide heterobifunctional derivatives bearing steroid residues is proposed.
Tumor Volume Versus Percentage of Specimen Involved by Tumor Correlated with Progression in Stage a Prostatic Cancer (1988)

Previous studies from this institution have shown that in untreated stage A prostate cancer when 5 per cent of the specimen or less was involved by tumor (stage A1) only 2 per cent of the patients had progression at 4 years. Of the specimens with greater than 5 per cent cancer (stage A2) 33 per cent had progression at 4 years. More recently, we have shown that 16 per cent of the men with stage A1 disease who remained at risk for 8 years or longer after diagnosis had progression of disease despite a small percentage of the specimen involved by tumor. To address whether the actual volume of tumor resected may be a better predictor of progression than the percentage of the specimen involved by tumor the data from these studies were re-evaluated. We demonstrated that at 4 and 8 years of followup the percentage of the specimen and resected tumor volume were strongly associated with tumor progression, with the percentage of the specimen showing a stronger association especially at 4 years. Our study reaffirms the use of the percentage of resected tissue involved by tumor as a means to distinguish between stages A1 and A2 prostate cancer. Although at 8 years the distinction between stages A1 and A2 disease is blurred, this staging classification still is useful to identify those tumors with a relatively indolent course and a minimal short-term risk of progression from those that are more aggressive.
Phase and Retinal Images Restoration by 3D Phase Diversity (2007)

We report on a myopic 3D deconvolution method developed in a Bayesian framework for retinal imaging. Several useful constraints are enforced, notably a longitudinal support constraint similar to the phase diversity technique.
Robust Design of Multivariable Feedback Systems with Real Parameter Uncertainty and Unmodelled Dynamics (1989)

This paper presents a design methodology for the synthesis of a robust controller that accounts for both unmodelled dynamics and structured real parameter uncertainty for multiple-input, multiple-output systems. In a design aimed at constraining both the H <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">∞</sub> norm of a certain disturbance transfer matrix and a quadratic gaussian performance index under their respective bounds, a surrogate system may be formed by modelling the structured real parameter uncertainty as additional noise inputs and additional weights at the existing noise inputs and measurement outputs of the system. Application of a Riccati equation approach to this surrogate system then yields a robust controller which, when used in the actual system, will result in a closed-loop system having the same H <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">∞</sub> bound and quadratic gaussian performance index bound as the surrogate system, even in the presence of given real parameter variations.
Electron-Ion Correlation in Liquid Sodium (1989)

The structure factor of liquid sodium at 110°C has been determined by neutron diffraction with sufficient accuracy and compared with those obtained by X-ray diffraction. A small but clear difference in these structure factors has been found in the region \(Q{\lesssim}5\) A -1 , and these differences are apparently larger than the experimental errors. The ion-electron correlation function has been evaluated from this difference with the help of theoretical calculation of the electron-electron correlation function. The radial distribution function of valence electron around ions has been calculated by the Fourier transform of this function and the different features of the electron charge distribution from those in liquid polyvalent metals are also discussed.
FLEXOR TENDON REPAIR (2002)

Two views of tendon healing’s capability have prevailed since the early 1940’s. This presentation will outline the change in perspective regarding the primary tendon repair potential of intrasynovial flexor tendons and the clinical innovations that have been devised to reduce the inflammatory response and to improve the functional and structural characteristics of repair. Specifically, advanced suture, rehabilitation, and salvage techniques for tendon injuries will be discussed.
Cell‐Mediated Immunity to <i>Plasmodium falciparum</i> Infection: Evidence against the Involvement of Cytotoxic Lymphocytes (1988)

Blood mononuclear cells (PBMC) recognizing soluble malaria antigens (SPag) are present in the peripheral blood of individuals clinically immune to malaria, and they proliferate after exposure to such antigens. To test whether these cells have effector activity against Plasmodium falciparum, we stimulated PBMC from malaria-immune donors by SPag and purified protein derivative (PPD) in culture for 7 days. The PBMC were then co-incubated with P. falciparum for 48 h, and parasitaemia was determined by microscopy. Parasite growth was only significantly impaired after incubation with PBMC stimulated by either SPag or PPD in the presence of immune serum. Studies on subpopulations of PBMC indicated that the inhibitory cells resided among the adherent cell fraction. Furthermore we tested PBMC for cytotoxic activity against P. falciparum-infected autologous or heterologous erythrocytes. Experiments were done both in the absence and the presence of immune serum. Neither fresh PBMC nor PBMC activated by SPag or PPD for 7 days prior to assay were cytotoxic, indicating that cytotoxic T cells, natural killer (NK) cells, and K cells did not possess cytotoxic activity directed against parasitized erythrocytes. These data support the hypothesis that activated monocytes are the most important effector cells in the peripheral blood of malaria immune individuals.
Shareholder Interests, Human Capital Investment and Corporate Governance / Интересы акционеров, инвестиции в человеческий капитал и управление корпорацией (2000)

Корпорации одновременно утверждают, что человеческий капитал все более важен для их успеха, и что они стремятся максимизировать биржевую стоимость акций. В этой статье исследуется связь между этими двумя обстоятельствами. Мы показываем, что преследование интересов акционеров может потребовать передачи некоторой роли в управлении корпорации трудовому коллективу с целью мотивировать их на инвестирование в специфичный для фирмы человеческий капитал. Такие действия становятся более привлекательными, поскольку важность этих инвестиций возрастает. Этот результат также имеет отношение к спорам о реформировании европейской и японской систем управления в направлении американской системы, где влияние трудового коллектива ниже. В этом контексте мы предлагаем модель оптимального выбора системы управления в духе идей, которые предложил Холмстром.
Cholinergic muscarinic receptors and airway remodelling in severe asthma (2018)

<b>Rationale:</b> In asthma airway hyperresponsiveness is modulated by muscarinic receptors, but in asthma remodelling their role is not yet defined. Aim of this study was to evaluate cholinergic M2 and M3 muscarinic receptors (M2r/M3r) expression and their relationship with vascular, matrix and neural remodelling in mild and severe asthma. <b>Methods:</b> M2r, M3r, VEGF and NGF were analysed by immunohistochemistry in cryostat sections of bronchial biopsies from healthy controls (n=10), mild (n=20) and severe (n=20) asthmatics. Localisation of procollagen III was evaluated by confocal immunofluorescence. Western blot analysis for M3r and NGF and ELISA for VEGF were performed on human bronchial primary fibroblast (HBPF) cultures of controls and asthmatics stimulated with acetylcholine. <b>Results:</b> In bronchial lamina propria the number of M2r<sup>+</sup>, M3r<sup>+</sup>, VEGF<sup>+</sup> and NGF<sup>+</sup> cells were higher in severe compared to mild asthmatics and controls (M2r: p<0.01; M3r: p<0.05 and p<0.01; VEGF: p<0.001; NGF: p<0.05 and p<0.001, respectively). M2r<sup>+</sup> and M3Rr<sup>+</sup> cells were related to FEV1 (Rs= -0.49, p<0.01; Rs=-0.43, p<0.05) and to VEGF+ (Rs=0.43, p<0.05; Rs=0.62, p<0.001) in asthmatics and each other (Rs=0,46; p=0.01). Confocal analysis showed that in severe asthmatics procollagen III expression appeared higher than control. Western blot demonstrated that acetylcholine stimulation of HBPF increased the expression of NGF and M3r in controls and in asthmatics cultures (p<0.05, p<0.01 respectively). ELISA revealed higher levels of VEGF after cholinergic stimulation in asthmatic compared to control HBPF (p<0.001). <b>Conclusions:</b> Cholinergic muscarinic receptors are involved in each different aspects of asthma remodelling in severe asthma.
Intravascular lymphomatosis a systemic disease with neurologic manifestations (1993)

The unexplained presence of any one or more of these neurologic syndromes should alert the physician to the possible presence of this disease.
Age Changes in the Interactions between the Accommodation and Vergence Systems (2001)

Experiments are described in which static and dynamic accommodation (Ar), accommodative convergence (AC), vergence (C) and convergence accommodation (CA) responses to small stimuli were measured dynamically in 13 subjects with ages in the range 16 to 48 years. Analysis showed that the amplitudes of both blur and disparity-driven accommodation declined significantly with age, whereas the two types of vergence response did not. As a result, the AC/Ar ratio rose significantly with age, whereas the CA/C ratio fell. No significant change with age was found in response latencies and durations.
Investigations on sterols XIV studies on vitamin D and related compounds XII the photoisomerization of lumisterol<sub>2</sub> (1960)

Abstract Kinetic experiments on the isomerization of lumisterol 2 under the influence of ultraviolet light of various wavelengths are reported. The experiments indicate that neither tachysterol 2 nor ergosterol are primary reaction products of lumisterol 2 . Lumisterol 2 is exclusively converted into pre‐ergocalciferol. The quantum yield of this reaction is 0.41 at 2537 Å.
Waferbond technologies and quality assessment (2008)

Several wafer bond technologies like direct and anodic bonding without intermediate layer or thermo-compression wafer bonding with intermediate layers like low melting frit glasses, eutectic materials or polymers were developed during the last years and are nowadays extensively used in industrial applications. The paper will describe some important aspects of new Ti-Si and Laser assisted transmission Si to Si bonding techniques and their application for the fabrication of an ultrasonic transducer array. Main aspects for the reliability of wafer bonded devices are the yield after bonding, the bond strength and the hermeticity as well as the suitability for the packaging processes (e.g. moulding) and the influence of electrical and mechanical properties of the bonded elements by the bond process. The paper will focus on methods to characterize the bond strength and the hermeticity of bonded compounds. The main method to evaluate the bond strength is the micro chevron test. This test requires special designed test samples and is destructive, but also standard chips with bonded caps can be used for strength measurements. For anodic bonding between transparent glass and silicon a non destructive bond strength test structure can be used. This non-destructive test structure based on structure fabricated on one surface of the bond partners and has dimensions of several 10 to 100 μm. After the anodic bonding the length of the not bonded gap around this structure can be used to calculate the bond strength. The hermeticity can be tested by helium leakage test or by using resonant structure inside of the cavities which change the frequency depending on the pressure inside of the cavity.
Characterization of Amorphous-Particle Haze (1981)

A number of hazes in worts and in beers before final filtration were examined with nephelometry, electronic particle counting, optical microscopy, scanning electron microscopy, chemical analysis, and enzyme treatment. The relative merits of the various techniques in avoiding artifact formation, estimating the quantity of haze present, and characterizing the haze material to determine its origin were assessed. The hazes were almost entirely protein and carbohydrate. The carbohydrate contained a high percentage of reducing sugars, indicating an average degree of polymerization of 4. A large portion of the carbohydrate was nonglucan. Substantial amounts of mannose were found in one of the wort samples and in two of the beer samples.
Neutrophil to Lymphocyte Ratio (NLR) at the Time of Transurethral Resection of Bladder Tumor: A Large Retrospective Study and Analysis of Racial Differences (2017)

<b>Introduction:</b> Neutrophil/lymphocyte ratio (NLR) is an indicator of systemic inflammation and has been proven to be associated with an increased risk of extravesical disease, decreased cancer specific survival and overall survival in bladder cancer patients. A large proportion of healthy African Americans have a WBC count that is persistently lower than the normal range defined for individuals of European ancestry, this condition has been called "benign ethnic neutropenia". The purpose of our study was to determine if NLR was different in patients of African ancestry (AA) vs European ancestry (EA) across different tumor grades and stages at the time of transurethral resection of bladder tumor(s) (TURBT). <b>Materials and Methods:</b> The records of consecutive patients who underwent TURBT were reviewed from the University of Wisconsin and the Atlanta Veterans' Administration Medical Center (2000-2012). NLR was compared across tumor stage, tumor grade and ethnicity. <b>Results:</b> 297 consecutive patients met study criteria. 89% and 86%, were males and of European ancestry (EA) respectively. NLRs were different across T-stages (Ta-2.5, T1-3.9, T2-3.8; <i>p</i> = 0.001). but not across tumor grades in Ta (LG-2.5 vs HG-3.9, <i>p</i> = 0.57). EA had higher NLRs than AA (3.4 vs 1.9; <i>p</i> < 0.001). <b>Conclusions:</b> Higher NLRs appear to be associated with more advanced tumor stage at the time of TURBT. Patients of African ancestry have lower NLRs across all tumor stages compared to patients of European ancestry. Ethnicity should be taken into account when interpreting the NLR in patients with bladder cancer.
Submersible-Mounted In-Situ Geotechnical Instrumentation: ABSTRACT (1981)

This DOI is not currently attached to any metadata records. DOIs can’t actually ever be deleted (they’re persistent), but sometimes our members create DOIs in error. We do have a process to approximate deletion which we follow only in rare cases where the DOI has been genuinely created in error, and most crucially, if the DOI has never been published anywhere online or in print and never otherwise distributed to or communicated with anyone (authors, readers, reviewers, etc.
Regional Activation of Myosin II in Cancer Cells Drives Tumor Progression via a Secretory Cross-Talk with the Immune Microenvironment (2019)

ROCK-Myosin II drives fast rounded-amoeboid migration in cancer cells during metastatic dissemination. Analysis of human melanoma biopsies revealed that amoeboid melanoma cells with high Myosin II activity are predominant in the invasive fronts of primary tumors in proximity to CD206<sup>+</sup>CD163<sup>+</sup> tumor-associated macrophages and vessels. Proteomic analysis shows that ROCK-Myosin II activity in amoeboid cancer cells controls an immunomodulatory secretome, enabling the recruitment of monocytes and their differentiation into tumor-promoting macrophages. Both amoeboid cancer cells and their associated macrophages support an abnormal vasculature, which ultimately facilitates tumor progression. Mechanistically, amoeboid cancer cells perpetuate their behavior via ROCK-Myosin II-driven IL-1α secretion and NF-κB activation. Using an array of tumor models, we show that high Myosin II activity in tumor cells reprograms the innate immune microenvironment to support tumor growth. We describe an unexpected role for Myosin II dynamics in cancer cells controlling myeloid function via secreted factors.
Imaging in nasopharyngeal carcinoma: the value of 18-Florine Fluorodeoxyglucose PET/CT in comparison to conventional imaging modalities CT and MRI (2009)

Imaging in nasopharyngeal carcinoma: the value of 18-Florine Fluorodeoxyglucose PET/CT in comparison to conventional imaging modalities CT and MRIBackground. The aim of the study was to evaluate the clinical usefulness of 18F Fluorodeoxyglucose (FDG) positron emission tomography / computed tomography (PET/CT) in the management of nasopharyngeal carcinoma (NPC) in comparison to conventional imaging modalities.Methods. This retrospective study was done at Ospedale Niguarda, Milan, Italy. Data were acquired from 24 NPC patients between May 2003 and December 2006. They had FDG PET/CT and CT or MRI during the initial diagnosis and at follow-up. Each finding was tabulated and compared with tissue biopsy at diagnosis and clinical status during the follow up after the therapy. A statistical calculation was done to derive the value of each modality.Results. The sensitivity and accuracy of PET/CT and CT/MRI were equally high at diagnosis. At the follow up, a negative PET/CT finding suggested a complete remission with sensitivity and negative predictive value of 100%.Conclusions. 18F FDG PET/CT is a potential modality to be utilized in following up NPC patients for evaluating a response to therapy.
Circuit and system design for an 860–960 MHz RFID reader front‐ends with Tx leakage suppression in 0.18 − µm CMOS technology (2011)

SUMMARY This paper presents an RF Front‐END for an 860–960thinspaceMHz passive RFID Reader. The direct conversion receiver architecture with the feedback structure in the RF front‐end circuit is used to give good immunity against the large transmitter leakage and to suppress leakage. The system design considerations for receiver on NF and IIP3 have been discussed in detail. The RF Front‐END contains a power amplifier (PA) in transmit chain and receive front‐end with low‐noise amplifier, up/down mixer, LP filter and variable‐gain amplifier. In the transmitter, a differential PA with a new power combiner is designed and fabricated in a 0.18‐µm technology. The chip area is 2.65 mm × 1.35 mm including the bonding pads. The PA delivers an output power of 29 dBm and a power‐added efficiency of 24% with a power gain of 20 dB, including the losses of the bond‐wires. Copyright © 2011 John Wiley & Sons, Ltd.
Stem Cells (2015)

Objective: The objective of this presentation is to create awareness of stem cell applications in the ISORBE community and to foster a strategy of how the ISORBE community can disseminate information and promote the use of radiolabeled stem cells in biomedical applications. Methods: The continued excitement in Stem Cells, in many branches of basic and applied biomedical science, stems from the remarkable ability of stem cells to divide and develop into different types of cells in the body. Often called as Magic Seeds, stem cells are produced in bone marrow and circulate in blood, albeit at a relatively low concentration. These virtues together with the ability of stem cells to grow in tissue culture have paved the way for their applications to generate new and healthy tissues and to replace diseased or injured human organs. Although possibilities of stem cell applications are many, much remains yet to be understood of these remarkable magic seeds. Conclusion: This presentation shall briefly cover the origin of stem cells, the pros and cons of their growth and division, their potential application, and shall outline some examples of the contributions of radiolabeled stem cells, in this rapidly growing branch of biomedical science.
Robotic Radical Prostatectomy for Patients with Locally Advanced Prostate Cancer Is Feasible: Results of a Single-Institution Study (2009)

Our results suggest that RP may be performed safely on patients with locally advanced PC.