gebhart/patrepeval huggingface dataset:
Efficient gene replacement and direct hyphal transformation in <i>Sclerotinia sclerotiorum</i> (2008)

Homologous recombination is required for gene-targeted procedures such as gene disruption and gene replacement. Ku80 is part of the non-homologous end-joining DNA repair mechanism in many organisms. We identified and disrupted the Ku80 homologue in Sclerotinia sclerotiorum and generated heterokaryon mutants enriched with Ku80-deficient nuclei (ssku80). Sclerotial formation and pathogenicity of ssku80 mutants were normal on tomato fruits. The frequencies of homologous recombination in these strains were much higher than those of the wild type when transformed with a cna1 (encoding calcineurin) replacement construct. We coupled the increase in homologous recombination with a direct BIM-LAB-mediated transformation procedure, which utilizes compressed air to assist the transforming DNA in penetrating fungal hyphae of S. sclerotiorum. We found this method to be efficient and reproducible, and it did not alter the fitness of the mutants. We also demonstrated the first case of direct transformation of sclerotia. Nourseothricin was introduced as a selectable marker in S. sclerotiorum. The tools and procedures described will improve our ability to study gene function in S. sclerotiorum and are most likely to be adaptable for use in other plant pathogens.
High Toughness and Light Transmittance of PMMA Composite Prepared via<i>In-Situ</i>Polymerization with Incorporating Self-Assembled Dendritic Gel Networks (2014)

High toughness and light transmittance PMMA composite was prepared via in-situ thermal polymerization with gel networks self-assembling from dendritic gelators. Significant improvements of PMMA composite's toughness could be achieved by adding a small amount of dendrimers, without losing its light transmittance. Phase morphology, mechanical properties and optical performance of the composite were investigated with SEM, DMTA, impact strength test and UV–Vis spectrophotometer, respectively. The results showed that gel networks constructed from the dendrimers’ self-assembling kept intact during in-situ polymerization, while the PMMA composite containing very small amount(≤1.0 wt%) of G2-C12-G2orgelator-1 exhibits higher storage modulus, toughness and glass transition temperature than those of PMMA homopolymer.
Tomographic bioluminescence imaging by use of a combined optical-PET (OPET) system: a computer simulation feasibility study (2005)

The feasibility and limits in performing tomographic bioluminescence imaging with a combined optical-PET (OPET) system were explored by simulating its image formation process. A micro-MRI based virtual mouse phantom was assigned appropriate tissue optical properties to each of its segmented internal organs at wavelengths spanning the emission spectrum of the firefly luciferase at 37 degrees C. The TOAST finite-element code was employed to simulate the diffuse transport of photons emitted from bioluminescence sources in the mouse. OPET measurements were simulated for single-point, two-point and distributed bioluminescence sources located in different organs such as the liver, the kidneys and the gut. An expectation maximization code was employed to recover the intensity and location of these simulated sources. It was found that spectrally resolved measurements were necessary in order to perform tomographic bioluminescence imaging. The true location of emission sources could be recovered if the mouse background optical properties were known a priori. The assumption of a homogeneous optical property background proved inadequate for describing photon transport in optically heterogeneous tissues and led to inaccurate source localization in the reconstructed images. The simulation results pointed out specific methodological challenges that need to be addressed before a practical implementation of OPET-based bioluminescence tomography is achieved.
A method for monolingual thesauri merging (1997)

article Free Access Share on A method for monolingual thesauri merging Authors: Marios Sintichakis Department of Computer Science, University of Crete and Institute of Computer Science, Foundation for Research and Technology - Hellas Department of Computer Science, University of Crete and Institute of Computer Science, Foundation for Research and Technology - HellasView Profile , Panos Constantopoulos Department of Computer Science, University of Crete and Institute of Computer Science, Foundation for Research and Technology - Hellas Department of Computer Science, University of Crete and Institute of Computer Science, Foundation for Research and Technology - HellasView Profile Authors Info & Claims ACM SIGIR ForumVolume 31Issue SIDecember 1997 pp 129–138https://doi.org/10.1145/278459.258551Published:01 July 1997Publication History 17citation515DownloadsMetricsTotal Citations17Total Downloads515Last 12 Months26Last 6 weeks6 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my Alerts New Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
Photocatalytic reduction of CO<sub>2</sub>and protons using water as an electron donor over potassium tantalate nanoflakes (2014)

CO2 photoreduction over KTaO3 photocatalysts in water has been investigated. Both reduction (mainly carbon monoxide and hydrogen) and oxidation products (oxygen) were simultaneously detected in the absence of cocatalysts, sacrificial reagents and electrical bias. KTaO3 (KTO) samples were synthesized by solid-state reaction (SSR-KTO) and solvothermal methods in a hexane-water mixture (Hex-KTO) and pure ethanol (Eth-KTO). The different synthesis methods were used to control particle sizes and morphologies of KTaO3 and their correlation towards photocatalytic activity has been further investigated. Strikingly, KTO prepared in a hexane-water mixture solvent represents a nanoflake-like structure, resulting in a seven fold increase in CO2 reduction and hydrogen evolution activities in comparison to conventional cubic structure KTO under UV light irradiation. CO with trace amounts of CH4 is produced from CO2 photoreduction. Upon addition of a silver cocatalyst on KTO, the reduction selectivity has been controlled to favour CO2 photoreduction, and the CO2 to CO yield has been doubled compared to bare KTaO3.
Models for iron-oxo proteins. Structures and properties of FeIIFeIII, ZnIIFeIII, and FeIIGaIII complexes with (.mu.-phenoxo)bis(.mu.-carboxylato)dimetal cores (1989)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTModels for iron-oxo proteins. Structures and properties of FeIIFeIII, ZnIIFeIII, and FeIIGaIII complexes with (.mu.-phenoxo)bis(.mu.-carboxylato)dimetal coresA. S. Borovik, Vasilios Papaefthymiou, Lucille F. Taylor, Oren P. Anderson, and Lawrence Que Jr.Cite this: J. Am. Chem. Soc. 1989, 111, 16, 6183–6195Publication Date (Print):August 1, 1989Publication History Published online1 May 2002Published inissue 1 August 1989https://pubs.acs.org/doi/10.1021/ja00198a032https://doi.org/10.1021/ja00198a032research-articleACS PublicationsRequest reuse permissionsArticle Views1551Altmetric-Citations151LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Ion‐Exchange‐Induced 2D–3D Conversion of HMA<sub>1−<i>x</i></sub>FA<sub><i>x</i></sub>PbI<sub>3</sub>Cl Perovskite into a High‐Quality MA<sub>1−<i>x</i></sub>FA<sub><i>x</i></sub>PbI<sub>3</sub> Perovskite (2016)

High-quality phase-pure MA<sub>1-x</sub> FA<sub>x</sub> PbI<sub>3</sub> planar films (MA=methylammonium, FA=formamidinium) with extended absorption and enhanced thermal stability are difficult to deposit by regular simple solution chemistry approaches owing to crystallization competition between the easy-to-crystallize but unwanted δ-FAPbI<sub>3</sub> /MAPbI<sub>3</sub> and FA<sub>x</sub> MA<sub>1-x</sub> PbI<sub>3</sub> requiring rigid crystallization conditions. Here A 2D-3D conversion to transform compact 2D mixed composition HMA<sub>1-x</sub> FA<sub>x</sub> PbI<sub>3</sub> Cl perovskite precursor films into 3D MA<sub>1-x</sub> FA<sub>x</sub> PbI<sub>3</sub> (x=0.1-0.9) perovskites is presented. The designed Cl/I and H/FA(MA) ion exchange reaction induced fast transformation of compact 2D perovskite film, helping to form the phase-pure and high quality MA<sub>1-x</sub> FA<sub>x</sub> PbI<sub>3</sub> without δ-FAPbI<sub>3</sub> and MAPbI<sub>3</sub> impurity. In all, we successfully developed a facile one-step method to fabricate high quality phase-pure MA<sub>1-x</sub> FA<sub>x</sub> PbI<sub>3</sub> (x=0.1-0.9) perovskite films by 2D-3D conversion of HMA<sub>1-x</sub> FA<sub>x</sub> PbI<sub>3</sub> Cl perovskite. This 2D-3D conversion is a promising strategy for lead halide perovskite fabrication.
Theory of Protein Solutions. II (1955)

The formal theory of the first paper of this series is extended here to include the binding of several different species of ions or molecules on a protein, aggregation in a protein solution, and solutions containing more than one type of protein molecule.
Peripheral projections of rat primary sensory neurons immunoreactive for neurotrophin 3 (1995)

Sensory neurons can be classified into subpopulations based on a variety of characteristics, including their morphology and physiological modalities. Whether any of these classifications correlates with neurotrophic sensitivities has not been determined. We have recently reported that a subpopulation of large diameter sensory neurons of the rat contain neurotrophin 3-like immunoreactivity (NT3-ir). In this study, we have further characterised NT3-ir sensory neurons by their size, segmental localization, and peripheral projections by combined techniques of retrograde tracing and immunohistochemistry. The size distribution showed that NT3-ir was localised to a subpopulation of large-diameter neurons ranging from 560 to 3,120 microns2. Greater numbers of NT3-ir neurons reside in trigeminal (43% of total), cervical (36%), and lumbar (39%) than in thoracic spinal ganglia (13-17%). In combination with Fluoro-Gold retrograde tracing, it was found that about 30% of sensory neurons projecting to the tibial muscle were NT3-ir, compared with 39% for tendon, 50% for whisker hair follicles, 17% for subdermis or epidermis, and only 1% for kidney or adrenal gland. These studies indicate that NT3-ir sensory neurons mainly project to skin and muscles but not viscera. Thus, the characterization of NT3-ir spinal sensory neurons suggests that large sensory neurons subserving proprioception and mechanoception require NT3 for the maintenance of normal function.
La technique du tuteurage vertical est-elle applicable en bananeraie ? (1970)

Tuteurage vertical des bananiers : méthode, intérêt. Cameroun
Selective memory deduplication for cost efficiency in mobile smart devices (2014)

Memory deduplication, which can remedy memory scarcity in mobile systems, can hardly be used due to its high computation cost. This paper proposes a computation efficient memory deduplication scheme that avoids unnecessary computations for memory deduplication. To make it efficient, the proposed scheme gets rid of pages, which are unlikely to be deduplicated, from the target of memory deduplication. This exclusion is performed in time-domain and in space-domain by exploiting the characteristics of mobile applications. The prototype implementation shows significant computation cost reduction while providing the same memory savings as in previous approaches. The saved memory is used for improving application launch time as well.
Using a script model to preserve the consistency within an UI design environment (2005)

Model-based User Interface (UI) development represents a significant evolution in the process of UI Design. Most of currently model-based methodologies start the acquisition process of the interaction description (concrete models) from the task, domain and user descriptions (abstract models). This is basically a process of elicitation and mapping of elements of a model in another one. The major drawback concerning this approach is the lack of definitions of appropriate mechanisms that automatically promote the actualization of the abstract models (source) facing modifications of the concrete models (target). Such lack of actualization introduces inconsistencies between the different models and makes it difficult and even impossible to create the final user interface by doing several iterations over the models being used. In this paper we have proposed mechanisms of maintenance of the consistency between interaction and task models within an UI design environment, MEDITE+. A script model that is intermediate between the model of the task and the model of the interaction supports the definition of these mechanisms. The effects in the involved models using these mechanisms are shown in practical examples.
Processing of Filamentous Bacteriophage Virions in Antigen-Presenting Cells Targets Both HLA Class I and Class II Peptide Loading Compartments (2003)

Virions of filamentous bacteriophage fd are capable of displaying multiple copies of peptide epitopes and generating powerful immune responses to them. To investigate the antigen processing mechanisms in human B cell lines used as antigen presenting cells, the major coat protein (pVIII) in intact virions was fluorescently labeled, and its localization in various intracellular compartments was followed using confocal microscopy. We show that the virions were taken up and processed to yield peptides that reach both the major histocompatibility complex (MHC) class II compartment and the endoplasmic reticulum. Moreover, when exposed to bacteriophages displaying a cytotoxic T lymphocyte (CTL) epitope from the reverse transcriptase of human immunodeficiency virus type-1 (HIV-1), B cells were lysed by specific cytotoxic lymphocytes. This confirms that filamentous bacteriophage virions are capable of being taken up and processed efficiently by MHC class I and class II pathways, even in nonprofessional antigen presenting cells. These remarkable features explain, at least in part, the unexpected ability of virions displaying foreign T-cell epitopes to prime strong T-helper-dependent CTL responses. These findings have important implications for the development of peptide-based vaccines, using filamentous bacteriophage virions as scaffolds.
DESIGN AND INVESTIGATION OF BROADBAND MONOPOLE ANTENNA LOADED WITH NON-FOSTER CIRCUIT (2010)

The possibility of using non-Foster circuit to expand the bandwidth of a monopole antenna is investigated theoretically. Beginning with an inductor-loaded monopole antenna resonating at difierent frequencies by changing the value of the loaded inductor, we show that a frequency-dependent inductor is needed to enhance the bandwidth of the monopole antenna. The curve for the reactance of the frequency-dependent inductor versus frequency is fltted, which enlightens us to use a non-Foster reactive circuit to realize the frequency-dependent inductor. Based on the above studies, a monopole antenna loaded with a non-Foster circuit is presented. Simulated results demonstrate that the input reactance of the loaded antenna becomes stable and approaches zero, which favors the impedance matching and extends the bandwidth to a certain extent. Finally, a passive (Foster) matching circuit is designed to improve the bandwidth further. A 0.69-m monopole antenna with 2.0:1 VSWR in the frequency range 30{150MHz is designed and investigated.
A novel approach to estimate power consumption using SystemC transaction level modelling (2015)

The increasing complexity of current day Systems-on-Chip and the rising market demands for low power devices has necessitated the need to perform power analysis of the complete system-on-chip at system level. This would facilitate selection of optimal system architecture and opportunity to optimize the selected system design for reduced power consumption in the silicon as much more design optimization opportunities are available at system level in contrast to Register Transfer Level (RTL). Transaction level model provides the conceptual implementation of the design at higher abstraction level above RTL and is available much early in the design cycle as against RTL, hence, is being used in industries for early software development and functional verification of the hardware. In this work, we present a methodology to estimate power consumption of an IP at transaction level by capitalizing the existing transaction level model of the corresponding IP. The proposed methodology makes use of SystemC transaction level model not specifically designed with power estimation in mind. We then validate the proposed method against RTL for accuracy and speed. The proposed approach enables estimation of dynamic power consumption and leakage power consumption with an error within 20% of RTL and total power consumption with an error within 12% with respect to RTL. In terms of speed-up, the proposed approach enables 20x faster simulation, hence, faster power estimation compared to estimating power at RTL. The accuracy and speed-up of the proposed approach are illustrated through experimental results.
Tensile and compression performance of superelastic NiTi tubing (2001)

Stress–strain curves have been established in tension and compression at 19 and 37°C for a NiTi tube with an A f temperature of -8.5°C. In tension, the first strain cycle exhibited the familiar flag shape but this gradually decayed on cycling into a sigmoidal curve with an accompanying build up of residual strain. Changes were rapid at first but slowed down to an approximately steady state condition after ∼40 cycles. In compression, sigmoidal stress–strain curves were observed from the beginning, but these also changed and residual strain built up on cycling. In order to account for the behaviour in the superelastic regime, attempts have been made to draw on interpretations that have been advanced for similar differences and changes on cycling in the shape memory regime.
A DLL With Dual Edge Triggered Phase Detector for Fast Lock and Low Jitter Clock Generator (2012)

A DLL based on a dual edge triggered phase detector (DET-PD) is proposed for a clock generator in low-power systems. The proposed DLL has a faster lock speed with the same loop dynamics compared to the conventional DLL based on a single-edge triggered phase detector (SET-PD). The proposed DET-PD solves the problem of a narrow capture range or low phase detector gain associated with the conventional DET-PD. In addition, the proposed duty cycle difference compensation circuit (DDC) prevents the increase in the phase offset when the two inputs to the DET-PD have different duty cycle. It also controls the DLL bandwidth to maintain the DLL jitter by controlling the negative edge delay difference tracking. Finally, the proposed duty cycle keeper (DCK) enlarges the duty cycle keeping range of the DLL output. The proposed DLL is fabricated using 0.18-μm process technology. It has an area of 0.035 mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> and a power consumption of 19 mW at 800 MHz operation. Its lock speed is over 1.9 times faster than that of the DLL based on the SET-PD without degrading the jitter.
The Fault Location Method of Distribution Network with Fault Indicators Based on Impedance (2015)

The structure of distribution network is complex, and multi-hybrid power lines such as wires and cables are used, which cause difficulties in accurate determination of fault distance. A new method to pinpoint faults was provided by this paper. The fault branch was located with the logic operation of the status information provided by the disconnect switches and wiring fault indicators, and the fault distance was accurately calculated by the impedance method. In this method, the equations were written, in which the fault point and fault positive sequence voltage were treated as the unknown quantity, and the fault distance was calculated by solving the equations. Simulate with ATP, and the data was processed with MATLAB to verify the feasibility of the algorithms in complex distribution network fault location. The results show that the location method has a good localization accuracy.
Nonlocal Signal and Noise in <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" display="inline" overflow="scroll"><mml:mrow><mml:mi mathvariant="sans-serif">T</mml:mi></mml:mrow></mml:math>-Shaped Lateral Spin-Valve Structures (2018)

In spintronics, lateral spin valves enable the manipulation of pure spin current without charge current. These systems are very interesting, particularly as magnetic field sensors for $e.g.$ read heads in hard disk drives. This paper uses a two-dimensional diffusive model to investigate signal and noise in such T-shaped devices. Three sources of noise are discussed, one of which is associated with possible thermal fluctuations in spin accumulation. Though their signal-to-noise ratio still needs to be improved to make these devices suitable for hard disk drives, this study offers important physical insight to guide that quest.
An Edible Acetylated Monoglyceride Coating for Poultry Meat Preservation (1963)

AN INCREASING percentage of poultry meat is being offered to consumers in a prepackaged form. Until recently a majority of such poultry has been packaged at the retail level after having been purchased packed in ice. These distribution procedures required the use and transportation of large quantities of ice, and the need for packaging supplies and facilities plus labor at numerous retail stores. With advances in processing and quality control procedures it is now feasible to package ready-to-cook poultry or cut-up pieces in processing plants, and to distribute such products rapidly enough to offer them to consumers in excellent condition. A coating which minimizes moisture loss and inhibits bacterial growth would contribute to the effectiveness of marketing ready-to-cook poultry meat. Acetylated monoglycerides have recently been approved for food coatings. Wood-mansee and Abbott (1958) dipped fryer drumsticks in a 2–1 mixture of Myvacet2 Type 5–00 and Type 9–40 …