gebhart/patrepeval huggingface dataset:
Endothelioma Cells Express and Secrete Endothelin-1 and Inhibit Platelet Aggregation through Nitric Oxide Formation (1994)

Transformed endothelial cells expressing the polyoma middle T oncogene (endothelioma cells) cause tumors in mice and recruit host endothelial cells without stimulating their proliferation. A role for the endothelial vasoactive mediators nitric oxide (NO) and endothelin-1 (ET-1) in tumor cell biology has been postulated. Thus, the existence of a synthesizing pathway for both mediators in endothelioma cell lines derived from both subcutaneous (sEnd.1) and thymic (tEnd.1) hemangiomas was investigated. Northern blot analysis demonstrated the expression of mRNA for ET-1 precursor in both tumor cell lines. Moreover, ET-1 immunoreactive material was found in culture supernatants and reverse phase HPLC of the conditioned media revealed two peaks corresponding to the retention times of synthetic ET-1 and big ET-1. Endothelioma cell lines strongly inhibited thrombin-induced platelet aggregation. The antiplatelet activity was cell-number dependent, and could be potentiated when endothelioma cells were stimulated with either carbachol (100 μM) or histamine (100 μM). In addition, both agonists evoked a transient rise in the intracellular [Ca2+] of these cells. Incubation of endothelioma cells in L-arginine deficient medium (24 h) or pretreatment with the NO synthase inhibitor Nω-nitro-L-arginine methyl ester (L-NAME, 100μM) significantly reduced their antiplatelet activity. The inhibitory effect of L-NAME was overcome by co-incubation with L- (100 μM) but not D-arginine (100 μM). Conversely, exposure of endothelioma cells to either the protein synthesis inhibitor cycloheximide (1 μM, 48 h) or the glucocorticoid dexamethasone (1 μM, 48 h) did not modify their antiplatelet activity. The presence of constitutive, endothelial NO-synthase was confirmed by western blot of cell extracts from both cell lines. These results demonstrate that both ET-1 and NO are synthesized by and released from transformed endothelial cells. They further suggest that the enzyme responsible for NO synthesis in endothelioma cells is constitutively expressed.
A Field Test of Distributed Acoustic Sensing for Ambient Noise Recording (2015)

Summary We present results from an intermediate scale field experiment conducted in December 2014, evaluating the use of Distributed Acoustic Sensing (DAS) for recording ambient noise generated by infrastructure use; the future aim of our effort is to utilize ambient noise for real-time geotechnical evaluation of soils beneath active infrastructure. We present details of the installation, recording comparisons of 4 selected DAS cables, and a noise comparison between DAS and classical 3C geophones. We conclude by showing preliminary examples of ambient noise interferometry using passive data acquired with DAS vs. 3C geophones.
In Silico Identification of Putative Regulatory Sequence Elements in the 5′-Untranslated Region of Genes That Are Expressed during Male Gametogenesis (2003)

During pollen development, transcription of a large number of genes results in the appearance of distinct sets of transcripts. Similar mRNA sets are present in pollen of both mono- and dicotyledonous plant species, which indicates an evolutionary conservation of genetic programs that determine pollen gene expression. In pollen, regulation of gene expression occurs at the transcriptional and posttranscriptional level. The 5'-untranslated region (UTR) of several pollen transcripts has been shown to be important for regulation of pollen gene expression. The important regulatory role of 5'-UTR sequences and the evolutionary conservation of genetic programs in pollen led to the hypothesis that the 5'-UTRs of pollen-expressed genes share regulatory sequence elements. In an attempt to identify these pollen 5'-UTR elements, a statistical analysis was performed using 5'-UTR sequences of pollen- and sporophytic-expressed genes. The analysis revealed the presence of several pollen-specific 5'-UTR sequence elements. Assembly of the pollen 5'-UTR elements led to the identification of various consensus sequences, including those that previously have been demonstrated to play a role in the regulation of pollen gene expression. Several pollen 5'-UTR elements were found to be preferentially associated to genes from dicots, wet-type stigma plants, or plants containing bicellular pollen. Moreover, three sequence elements exhibited a preferential association to the 5'-UTR of pollen-expressed genes from Arabidopsis and Brassica napus. Functional implications of these observations are discussed.
[Value of cytapheresis in the treatment of loaiasis with high blood microfilaria levels. Results in 7 cases]. (1987)

Seven patients infected with the filarial worm Loa loa received a treatment by cytapheresis in an attempt to lower the microfilaraemia. Microfilarial levels of between 6,000 and 38,500 ml, before extraction, were reduced, according to the case, by between 47 and 97% (mean 76%). The diethylcarbamazine chemotherapy which followed in 6 of 7 patients showed no sign of any of the serious side-effects which often occur in these type of cases. Due to its practicality and the fact that it is well tolerated, both clinically and biologically, cytapheresis would seem to represent the best method for initially treating loaiasis with high microfilaremia.
A microprocessor-oriented instrument for fast and accurate measurement of analogue quantities in high-voltage stations (1980)

Presents a theoretical and experimental study of a bay equipment measuring analogue quantities (U, I, P, Q) in HV stations. The key to the system is microprocessor assisted handling of sampled data. A basis accuracy of 0.1% is obtained with a total refresh cycle time better than 0.5 s for one feeder.
A Difference Limen for Vowel Formant Frequency (1955)

Experiments have been conducted to determine difference limens (DL's) for vowel formant frequency. The DL's are obtained from quality judgments on synthetic vowel sounds. The results indicate the maximum accuracy necessary in analyzing the formant structure of spoken vowels and in synthesizing the sounds from the resulting formant data.
Optimization of cultivation conditions in spin tubes for Chinese hamster ovary cells producing erythropoietin and the comparison of glycosylation patterns in different cultivation vessels (2010)

This article describes the optimization of cultivation factor settings, that is the shaking rate and working volume in 50 mL spin tubes for a Chinese hamster ovary cell line expressing recombinant human alpha-erythropoietin, using a response D-optimal surface method. The main objectives of the research were, firstly, to determine a setting in which the product titer and product quality attributes in spin tubes are equivalent to those in 250 mL shake flasks in a seven day batch and, secondly, to find a setting in which the product titer is maximal. The model for product titer prediction as a function of shaking rate and working volume in the defined design space was successfully applied to the optimization of cultivation conditions in spin tubes for the tested cell line. Subsequently, validation experiments were carried out simultaneously in spin tubes, shake flasks and bench scale bioreactors to compare cell culture performance parameters such as growth, productivity and product quality attributes in the form of isoform profiles and glycan antennarity structures. The results of the experiments showed that similar cell culture performance and product quality could be achieved in spin tubes when compared to shake flasks. Additionally, bioreactor titers could be reproduced in spin tubes at high shaking rates and low working volumes, but with differing product quality. Cultivation at lower shaking rates in spin tubes and shake flasks produced a glycoprotein with a product quality slightly comparable to that from bioreactors, but with titers being only two thirds.
Retinoic Acid Receptor (RAR)-α Is Not Critically Required for Mediating Retinoic Acid Effects in the Developing Mouse Retina (2010)

Although RARalpha mediates RARE reporter transgene activity in the neural retina, its function is not necessary for the retina to develop and function normally. These data suggest that retinoic acid regulates neural retinal development through other, possibly RAR-independent, pathways.
Characterization of novolac resins based on <i>p</i>‐<i>tert</i>‐butylphenol by PMR spectroscopy (1976)

Abstract The direct silylation of tert ‐butylphenol formaldehyde resins with N , O ‐bis(trimethylsily)acetamide (BSA) has allowed a more complete analysis of these resins to be made with proton magnetic resonance (PMR) spectrometry. A quantitative determination of mixtures of the monomer, dimer, trimer, and tetramer may be carried out at 100 MHz.
Nevirapine pharmacokinetics and risk of rash and hepatitis among HIV-infected sub-Saharan African women (2012)

In this study, HIV-infected African women starting a NVP-based antiretroviral regimen had a lower NVP clearance compared to previous reports. Severe rash, but not hepatotoxicity, was associated with higher NVP exposure. Albeit observed in a small number of women, baseline CD4 cell count at least 250 cells/μl was significantly associated with NVP toxicity.
Probing Material Formation in the Presence of Organic and Biological Molecules (2001)

Mineralization in living organisms generates structures different from abiotic crystal growth. Mimicking biomineralization in vitro may become a way of synthesizing inorganic materials with useful microstructures. For example, the growth of calcium carbonate crystals in the presence of certain sea urchin proteins gives rise to microporous crystal lattices. Addition of organic chemicals, e.g., hexanol, modifies the structure and yields defect patterns.
Guest‐Induced Supramolecular Isomerism in Inclusion Complexes of T‐Shaped Host 4,4‐Bis(4′‐hydroxyphenyl)cyclohexanone (2005)

The T-shaped host molecule 4,4-bis(4'-hydroxyphenyl)cyclohexanone (1) has an equatorial phenol group and a cyclohexanone group along the arms and an axial phenol ring as the stem. The equatorial phenyl ring adopts a "shut" or "open" conformation, like a windowpane, depending on the size of the guest (phenol or o/m-cresol), for the rectangular voids of the hydrogen-bonded ladder host framework. The adaptable cavity of host 1 expands to 11x15-18 A through the inclusion of water with the larger cresol and halophenol guests (o-cresol, m-cresol, o-chlorophenol, and m-bromophenol) compared with a size of 10x13 A for phenol and aniline inclusion. The ladder host framework of 1 is chiral (P2(1)) with phenol, whereas the inclusion of isosteric o- and m-fluorophenol results in a novel polar brick-wall assembly (7x11 A voids) as a result of auxiliary C-H...F interactions. The conformational flexibility of strong O-H...O hydrogen-bonding groups (host 1, phenol guest), the role of guest size (phenol versus cresol), and weak but specific intermolecular interactions (herringbone T-motif, C-H...F interactions) drive the crystallization of T-host 1 towards 1D ladder and 2D brick-wall structures, that is, supramolecular isomerism. Host 1 exhibits selectivity for the inclusion of aniline in preference to phenol as confirmed by X-ray diffraction, 1H NMR spectroscopy, and thermogravimetry-infrared (TG-IR) analysis. The T(onset) value (140 degrees C) of aniline in the TGA is higher than those of phenol and the higher-boiling cresol guests (T(onset)=90-110 degrees C) because the former structure has more O-H...N/N-H...O hydrogen bonds than the clathrate of 1 with phenol which has O-H...O hydrogen bonds. Guest-binding selectivity for same-sized phenol/aniline molecules as a result of differences in hydrogen-bonding motifs is a notable property of host 1. Host-guest clathrates of 1 provide an example of spontaneous chirality evolution during crystallization and a two-in-one host-guest crystal (phenol and aniline), and show how weak C-H...F interactions (o- and m-fluorophenol) can change the molecular arrangement in strongly hydrogen-bonded crystal structures.
Distributed design of universal lightweight RFID system for large-scale RFID operation (2011)

The design of Radio Frequency Identification RFID network [1], [3-5], [7], [9], [11], [12] hence plays a significant role for successfully deploying large-scale applications [7], [10], [13], [14]. This is especially true when the tagged object is on item-level. This paper discusses issues that should be considered in design of RFID network; analyzes the potential impacts of different RFID network design on the existing enterprise network [8] and presents the Distributed Design of Universal lightweight RFID system [2], [6] for Large-Scale RFID operation for different RFID deployment environment [13-16] and computing platforms, which has the advantage of the rapid development of new scene-oriented RFID system. Customization and reconfiguration with lightweight [2], [4-6] capabilities are the aspirations of our developed RFID distributed system for large scale operation. Based on OSGi Open Service Gateway Initiative technology [17-19] and its Service-Oriented development mode, the paper describes the distributed service component framework and components design method for universal RFID system.
Shape-Pure, Nearly Monodispersed CsPbBr<sub>3</sub> Nanocubes Prepared Using Secondary Aliphatic Amines (2018)

Fully inorganic cesium lead halide perovskite (CsPbX<sub>3</sub>) nanocrystals (NCs) have been extensively studied due to their excellent optical properties, especially their high photoluminescence quantum yield (PLQY) and the ease with which the PL can be tuned across the visible spectrum. So far, most strategies for synthesizing CsPbX<sub>3</sub> NCs are highly sensitive to the processing conditions and ligand combinations. For example, in the synthesis of nanocubes of different sizes, it is not uncommon to have samples that contain various other shapes, such as nanoplatelets and nanosheets. Here, we report a new colloidal synthesis method for preparing shape-pure and nearly monodispersed CsPbBr<sub>3</sub> nanocubes using secondary amines. Regardless of the length of the alkyl chains, the oleic acid concentration, and the reaction temperature, only cube-shaped NCs were obtained. The shape purity and narrow size distribution of the nanocubes are evident from their sharp excitonic features and their ease of self-assembly in superlattices, reaching lateral dimensions of up to 50 μm. We attribute this excellent shape and phase purity to the inability of secondary amines to find the right steric conditions at the surface of the NCs, which consequently limits the formation of low-dimensional structures. Furthermore, no contamination from other phases was observed, not even from Cs<sub>4</sub>PbBr<sub>6</sub>, presumably due to the poor ability of secondary aliphatic amines to coordinate to PbBr<sub>2</sub> and, hence, to provide a reaction environment that is depleted in Pb.
Mutations in the C-Terminal Domain of Sonic Hedgehog Cause Holoprosencephaly (1997)

Holoprosencephaly (HPE) is the most common brain anomaly in humans, involving abnormal formation and septation of the developing central nervous system. Among the heterogeneous causes of HPE, mutations in the Sonic Hedgehog (SHH) gene have been shown to result in an autosomal dominant form of the disorder. Here we describe a total of five different mutations in the processing domain encoded by exon 3 of SHH in familial and sporadic HPE. This is the first instance in humans where SHH mutations in the domain responsible for autocatalytic cleavage and cholesterol modification of the N-terminal signaling domain of the protein have been observed.
Abstract 12797: Temporal Changes in Mortality Risk Post-Discharge After Hospitalization for Heart Failure (2014)

Importance: In observational studies, the risk for adverse events among patients admitted for heart failure (HF) is highest immediately post-discharge and declines over time. This ‘vulnerable phase’ concept has led to research and quality of care initiatives focusing primarily on early post-discharge period. However, whether this risk trajectory represents lowering of individual patient risk or changes in the population risk profile is not known. Methods: Survival and longitudinal models were used to assess temporal changes in mortality risk post-discharge in the Efficacy of Vasopressin Antagonism in Heart Failure Outcome Study With Tolvaptan (EVEREST) trial. Post hoc analysis of EVEREST included 4017 patients >18 years old discharged alive after hospitalization for HF with an ejection fraction of <40% at 359 sites from 2003-2006 and randomized to receive oral tolvaptan or placebo. Results: After a median of 9.8 months, 971 patients died (24.2%). Mortality at 1 year was 24.2%. Cumulative mortality rate declined from 15.9/100 patient-years immediately post-discharge to 13.4 at 30 days and 12.8 at 90 days; it rose steadily thereafter. The risk variation between quintiles of baseline risk was considerably larger than the temporal variation within risk strata. In a longitudinal model assessing updated mortality risk after each follow up visit, mortality risk increased during the 90 days preceding readmission and then followed a trajectory similar to the index admission. The net increase in mortality attributable to readmission was ~37%. Conclusions and Relevance: Among hospitalized HF patients, although there is a transiently elevated risk in 90 days before and after discharge, the patient’s clinical risk profile rather than temporal change remains the main determinant of mortality risk.
Statistical Properties of a 3-Wave System (1985)

Statistical properties of a 3-wave system are investigated numerically and a comparison is made with the results calculated by approximations in turbulence theory. It is found that evolution of energy and distribution of amplitude are affected by higher order moments of initial distribution except chaotic cases. In some cases, the approximations are found to be qualitatively poor.
RNA Polymerase II subunit 3 is retained in the cytoplasm by its interaction with HCR, the psoriasis vulgaris candidate gene product (2005)

Here, we show that the subcellular localization of alpha-like RNA polymerase II core subunit 3 (RPB3) is regulated during muscle differentiation. We have recently demonstrated that the expression of RPB3 is regulated during muscle differentiation and that, inside RNA polymerase II (RNAP II), it is directly involved in contacting regulatory proteins such as the myogenic transcription factor Myogenin and activating transcription factor ATF4. We show for the first time, that RPB3, in addition to its presence and role inside the RNAP II core enzyme, accumulates in the cytoplasm of cycling myogenic cells and migrates to the nucleus upon induction of the differentiation program. Furthermore, using human RPB3 as bait in a yeast two-hybrid system, we have isolated a novel RPB3 cytoplasmic interacting protein, HCR. HCR, previously identified as alpha-helix coiled-coil rod homologue, is one of the psoriasis vulgaris (PV) candidate genes. In cycling myogenic C2C7 cells, we show that the RPB3 protein directly interacts with HCR within the cytoplasm. Finally, knocking down HCR expression by RNA interference, we demonstrate that HCR acts as cytoplasmic docking site for RPB3.
Molecular biology in cytopathology (2000)

The use of molecular techniques in cytopathology has become an accepted and billable practice for certain applications. Other applications still are in the developmental or experimental stages but may soon be clinically useful. The current study provides a review of these techniques including molecular detection of clonality, in situ hybridization, loss of heterozygosity, and single base mutation detection. A number of new molecular techniques recently have been described that may have a dramatic impact on diagnostic pathology. These techniques, in situ detection of single base mutations and microarray technology, are introduced and discussed in the context of potential applications to cytopathology in the future.
Insight into the S/D engineering by high-resolution imaging and precise probing of 2D-carrier profiles with scanning spreading resistance microscopy (2009)

For the first time, high-resolution carrier imaging has been carried out on (110)/(100) pFETs and nFETs with scanning spreading resistance microscopy (SSRM). The S/D of (110) pFETs shows less lateral distribution than that of (100), strongly indicating 2D-channeling effect of boron I/I. Direct evidence has been shown that As out-diffusion under NiSi made conductive paths that degrade junction leakage on (110) nFETs. The Si:C influences on S/D profiles are also directly observed. We also succeeded in a full-FIB sample-making for the first time, showing the high potential of SSRM technology for further scaled devices.
CDK-Mediated Regulation of Cell Functions via c-Jun Phosphorylation and AP-1 Activation (2011)

Cyclin-dependent kinases (CDKs) and their targets have been primarily associated with regulation of cell-cycle progression. Here we identify c-Jun, a transcription factor involved in the regulation of a broad spectrum of cellular functions, as a newly recognized CDK substrate. Using immune cells from mouse and human, and several complementary in vitro and in vivo approaches including dominant negative protein expression, pharmacologic inhibitors, kinase assays and CDK4 deficient cells, we demonstrate the ability of CDK4 to phosphorylate c-Jun. Additionally, the activity of AP-1, a ubiquitous transcription factor containing phosphorylated c-Jun as a subunit, was inhibited by abrogating CDK4. Surprisingly, the regulation of c-Jun phosphorylation by CDK4 occurred in non-dividing cells, indicating that this pathway is utilized for cell functions that are independent of proliferation. Our studies identify a new substrate for CDK4 and suggest a mechanism by which CDKs can regulate multiple cellular activation functions, not all of which are directly associated with cell cycle progression. These findings point to additional roles of CDKs in cell signaling and reveal potential implications for therapeutic manipulations of this kinase pathway.
Photocurrent near‐field microscopy of Schottky barriers (1999)

We used a combination of internal photoemission and of near-field optical microscopy (SNOM) to study the lateral variations in solid interface properties such as energy barriers and electron-hole recombination. In particular we investigated the fully formed Pt-GaP, Au-GaAs, Au-SiNx-GaAs and PtSi-Si Schottky barriers. Our approach enabled us to measure large lateral variations in the photocurrent with spatial resolution on the nanometric scale. Due to the ability of SNOM to supply parallel topographic information, we observed photocurrent variations from zone to zone that only correlated in a few cases with local variations in surface morphology. We assigned the uncorrelated fluctuations to local variations in the interface stoichiometry, the presence of interface states induced by the metallic overlayer and to defect states at the junction. Furthermore, by tuning the photon energy and applied bias we were able to measure the surface distribution of the diffusion length.