1196 CIRCULATING ROLLING PROSTATE CANCER CELLS POTENTIATE METASTASIS: A MICRO-CHANNEL MODEL (2010)

You have accessJournal of UrologyProstate Cancer: Basic Research VI1 Apr 20101196 CIRCULATING ROLLING PROSTATE CANCER CELLS POTENTIATE METASTASIS: A MICRO-CHANNEL MODEL Sayeda Karim-Yasmin, Jong-Wei Hsu, Huei-Ju Ting, Edward Messing, and Yi-Fen Lee Sayeda Karim-YasminSayeda Karim-Yasmin More articles by this author , Jong-Wei HsuJong-Wei Hsu More articles by this author , Huei-Ju TingHuei-Ju Ting More articles by this author , Edward MessingEdward Messing More articles by this author , and Yi-Fen LeeYi-Fen Lee More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2010.02.697AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Cancer metastasis is the most dangerous and life-threatening phase of the disease. Thus, identification of the pathways that control cancer metastasis could facilitate strategies for development of early detection and treatment of cancer before invasion and metastasis. Adhesion between prostate cancer (PCa) cells and endothelial cells is a critical event for PCa cells to metastasize to distant organs. The objective of this study is to isolate subpopulations of circulating PCa cells that possess higher rolling and adhesion capacity, then confirm their cellular metastasis behavior. Also, molecular networks that control PCa metastasis will be defined. METHODS A dynamic flow-based fractionation of PCa cells into adhesion (rolling) and non-adhesion (floating) subpopulations was performed. The gene profiles between these two subpopulations were compared using cancer metastasis focus Q-PCR array. The cellular metastasis potential was further confirmed by invasion and migration assays as well as an orthotopic implantation nude mice model. RESULTS This dynamic microchannel model can capture a small population of PCa cells that have higher metastatic characteristics during the circulation phase. This subpopulation of PCa cells: rolling cells, indeed display much more aggressive behaviors in both in vitro assays (invasion and migration) and in vivo orthotopic PCa implantation mouse models. Gene profile analysis further confirmed that 29/84 metastatic-promoting genes were up-regulated in this rolling PCa subpopulation. CONCLUSIONS Using this flow-based dynamic system we were able to capture a small portion of circulating PCa cells that possess higher metastatic potential. This strategy provides a powerful way to capture and interrupt PCa adhesion to the blood vessel during circulation, which might eventually slow down PCa progression. Furthermore, this dynamic functional fractionation system could be further extended as a general approach for sorting of diverse cancer cell populations based on differences in functional adhesion properties. Rochester, NY© 2010 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 183Issue 4SApril 2010Page: e463 Advertisement Copyright & Permissions© 2010 by American Urological Association Education and Research, Inc.MetricsAuthor Information Sayeda Karim-Yasmin More articles by this author Jong-Wei Hsu More articles by this author Huei-Ju Ting More articles by this author Edward Messing More articles by this author Yi-Fen Lee More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...