gebhart/patrepeval huggingface dataset:
High Definition MALDI Imaging: New Analytical Capabilities for Protein Biomarker Discovery from Tissue (2010)

RP-44 MALDI imaging provides molecular information from tissue and to detect biomarkers. Lateral resolution (“pixel size”) of MALDI images determines the level of morphological correlation between molecular and histological image. Therefore, increasing the image resolution at no loss in signal intensity is paramount for a method that is meant to provide clinically relevant results. Lateral resolution is limited by sample preparation protocols, laser properties and instrument sensitivity. In addition, high acquisition (multiple pixesl/second) speed is required to keep measurement times for high resolution images in reasonable limits. We developed a novel laser design in a new axial MALDI-TOF instrument, which enables 20μm image resolution for proteins at 1 kHz laser repetition rate. Using classical Nd:YAG-solid state lasers with Gaussian energy profiles, we observed a dramatic loss in signal intensity from MALDI images - even at focus diameters exceeding 100 μm. Smartbeam solid state lasers employ a structured, non-Gaussian beam profile instead that counteracts this limitation efficiently. Until recently, this effect was limited to larger (∼80μm) focus sizes, but the redesigned smartbeam II optics used in the present study allow beam profile modulation down to 10μm while maintaining an efficient readout, which is a prerequisite for high definition MALDI imaging. We introduce rat testis tissue as an excellent model system to demonstrate lateral resolution in a MALDI imaging experiment. A single tissue cross-section shows numerous seminiferous tubules of ∼200μm diameter. Different stages of spermatid development are present simultaneously, but can only be visualized in a MALDI image if the resolution is sufficient to separate tubule wall and spermatocytes based on distinct molecular signals (∼20 ′ 20 μm pixel size). In addition, we demonstrate the importance of performing post-acquisition histological staining of the same section and co-registering digital photographs at microscopic resolution (from a Zeiss MIRAX scanner) for the interpretation of MALDI images.
English Ivy (<i>Hedera helix</i>) Control with Postemergence-Applied Herbicides (2013)

Abstract English ivy ( Hedera helix ) is an evergreen, perennial vine that was introduced from Europe and Asia and is not endemic in much of the United States. English ivy can be invasive and difficult to control once established. Four similar, but not identical, experiments were conducted in sequence to evaluate selected, POST-applied herbicides for English ivy control. English ivy plants were propagated from cuttings and container-grown to obtain a large population of uniform plants. Aminopyralid and fluroxypyr applied at 1.34 and 0.71 kg ae ha −1 , which is more twice the maximum registered rate for either herbicide, were ineffective. Glyphosate and 2,4-D amine were generally more effective, but neither herbicide provided a level of control that could be deemed consistently acceptable. Glyphosate applied at 8.51 kg ae ha −1 (the highest rate evaluated) provided 69, 98, and 89% control in the second, third, and fourth experiments as determined by foliage fresh-weight reduction relative to a nontreated control. Treatment with 2,4-D at 5.60 kg ae ha −1 (the highest rate evaluated) controlled English ivy 28, 98, and 89% in the second, third, and fourth experiments, respectively. Mixtures of 2,4-D and glyphosate were generally no more effective than were the components applied alone. Metsulfuron was the most effective herbicide. Metsulfuron applied at 0.168 kg ai ha −1 controlled English ivy ≥ 97% across the three experiments in which this treatment was included. This treatment also prevented regrowth.
Alterations in enteric nerve and smooth-muscle function in inflammatory bowel diseases (1997)

: Although there is much focus on the factors that lead to the expression of human inflammatory bowel disease (IBD), with a view to identifying the cause(s) and cure, work is still required to better understand the pathophysiology of the disease, with views to developing innovative treatment approaches for those patients with established and often complicated disease. This review examines the nature and extent of enteric nerve and smooth-muscle involvement in IBD. Parallels are drawn with recent animal studies, which clearly demonstrate that the deeper neuromuscular tissues of the gut are rapidly and profoundly altered after even superficial degrees of inflammation in the mucosa, and that this can occur without penetration of these tissues by the inflammatory response. In the enteric nervous system, there are quantitative and qualitative changes in neurotransmitter content, and recent work has shown that these agents (e.g., substance P) possess proinflammatory properties, whereas other (e.g., calcitonin gene-related peptide) possess antiinflammatory actions. Thus, the balance of neurotransmission clearly is important in determining the expression of diseases. With respect to muscle, inflammation-induced changes lead to alterations in contractility, which contribute to altered motility. There are also trophic changes and collagen formation that contribute to strictures of Crohn's disease. More recent work has focused on the ability of muscle to influence immune function through cytokine production, antigen presentation, and adhesion molecule expression to activate T lymphocytes. Thus, the neuromuscular tissues act not just as "innocent bystanders" but also as "active participants" in the inflamed gut.
Hematologic effects induced by periodic exposures of monkeys (Macaca mulatta) to gamma rays. (1961)

Twenty-three monkeys (Macaca mulatta) were divided into four groups and exposed periodically to low doses of gamma rays from Cow. The irradiated groups received 154, 274, and 590 rep total dose which was divided into doses administered every four days for 40 exposures. The cellular elements of the peripheral blood, hemoglobin. and hematocrit values were sampled and enumerated at intervals postirradiation. An analysis of variance was performed on the data from those adimals surviving to 24 weeks postirradiation. These surviving monkeys re-established normal hematologic values by the end of the 24-week period. No statistically significant results were obtdined for any parameter measured which would be interpreted as a radiation effect. (auth)
New Somatic Hybrid Rootstock Candidates for Tree-size Control and High Juice Quality (2011)

New citrus production systems that feature high density plantings being developed and adapted for Florida growing conditions will require improved tree-size controlling rootstocks that bear early and produce good yields of fruit with juice of high quality. Flying Dragon [Poncirus trifoliata(L.) Raf.], the standard dwarfing stock of the industry, grows slowly in the nursery and generally does not yield adequately. Improved alternative rootstock choices are desirable. Tetraploid rootstocks, both autotetraploids and allotetraploids, have been shown to have a capacity to reduce tree size when budded with commercial diploid scions. We have been exploring somatic hybridization of complementary diploid rootstocks via protoplast fusion to generate allotetraploid rootstock candidates for many years. Our early work featured production of somatic hybrids of many complementary rootstock combinations, including hybrids of sweet orange and mandarins with Flying Dragon. Field testing of such hybrids showed excellent tree size control, good yields, and juice quality; however, there were usually one or more problems with each hybrid, such as inadequate seed production for standard propagation, or poor tree survival at one or more locations. We have subsequently produced additional somatic hybrids of complementary rootstocks that appear to have solved these problems, which also include parentage for coldhardiness. Although replicated field trials of these somatic hybrid rootstock candidates were destroyed by the state-run canker eradication program, we were able to obtain yield and juice quality data over three consecutive seasons from a small blight tolerance trial in St. Cloud in collaboration with Mr. Orie Lee. Features of five such tree-size controlling somatic hybrid rootstock candidates will be presented, including information on seed production, tree size, yield, and juice quality. For example, the somatic hybrid of ‘Changsha’ mandarin + 50-7 trifoliate orange consistently produces 2.25 boxes of ‘Valencia’ fruit per tree with high juice quality on trees of similar size to those budded to Flying Dragon. These new somatic hybrid rootstocks should have good potential in emerging advanced production systems for Florida.
An Open-Source Tool for Automated Power Grid Stress Level Prediction at Balancing Authorities (2018)

The behavior of modern power systems is becoming more stochastic and dynamic, due to the increased penetration of variable generation, demand response, new power market structure, extreme weather conditions, contingencies, and unexpected events. It is critically important to predict potential system operational issues so that grid planners and operators can take preventive actions to mitigate the impact, e.g., lack of operational reserves. In this paper, an innovative software tool is presented to assist power grid operators in a balancing authority in predicting the grid stress level over the next operating day. It periodically collects necessary information from public domain such as weather forecasts, electricity demand, and automatically estimates the stress levels on a daily basis. Advanced Neural Network and regression tree algorithms are developed as the prediction engines to achieve this goal. The tool has been tested on a few key balancing authorities and successfully predicted the growing system peak load and increased stress levels under extreme heat waves.
ChemInform Abstract: KINETICS OF THE OXIDATION OF HYDROGEN PEROXIDE BY BIS(2,2′‐BIPYRIDINE)SILVER(II) IONS (1981)

Abstract Die Stöchiometrie der Reaktion zwischen H 2 O 2 und [Ag(bipy)2]2 (bipy: 2,2′‐Bipyridin) entspricht der in Reaktion A. Als reaktive Ag(II)‐Hauptspezies wird das mit [Ag(bipy)2]" im Gleichgewicht stehende [Ag(bipy)]" ermittelt.
Acute Neutral Endopeptidase Inhibition in Patients with Normal Glomerular Filtration Rate (1992)

Conference Abstract| July 01 1992 Acute Neutral Endopeptidase Inhibition in Patients with Normal Glomerular Filtration Rate GW Lipkin; GW Lipkin 1Renal Research Laboratory. St. Bartholomew's Hospital. London Search for other works by this author on: This Site PubMed Google Scholar ABS Dawnay; ABS Dawnay 1Renal Research Laboratory. St. Bartholomew's Hospital. London Search for other works by this author on: This Site PubMed Google Scholar SM Harwood; SM Harwood 1Renal Research Laboratory. St. Bartholomew's Hospital. London Search for other works by this author on: This Site PubMed Google Scholar WR Cattell; WR Cattell 1Renal Research Laboratory. St. Bartholomew's Hospital. London Search for other works by this author on: This Site PubMed Google Scholar AEG Raine AEG Raine 1Renal Research Laboratory. St. Bartholomew's Hospital. London Search for other works by this author on: This Site PubMed Google Scholar Author and article information Publisher: Portland Press Ltd Online ISSN: 1470-8736 Print ISSN: 0143-5221 © 1992 The Biochemical Society and the Medical Research Society1992 Clin Sci (Lond) (1992) 83 (s27): 27P. https://doi.org/10.1042/cs083027Pa Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Cite Icon Cite Get Permissions Citation GW Lipkin, ABS Dawnay, SM Harwood, WR Cattell, AEG Raine; Acute Neutral Endopeptidase Inhibition in Patients with Normal Glomerular Filtration Rate. Clin Sci (Lond) 1 July 1992; 83 (s27): 27P. doi: https://doi.org/10.1042/cs083027Pa Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsClinical Science Search Advanced Search This content is only available as a PDF. © 1992 The Biochemical Society and the Medical Research Society1992 Article PDF first page preview Close Modal You do not currently have access to this content.
Author Correction: Weight Bearing Activities change the Pivot Position after Total Knee Arthroplasty (2020)

An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Allosteric regulation by non peptidic, low molecular weight compounds of CCR5 coupling to g-proteins and interaction with Gp120 - consequences on inhibition of R5 HIV-1 infection (2010)

Allosteric regulation by non peptidic, low molecular weight compounds of CCR5 coupling to g-proteins and interaction with Gp120 consequences on inhibition of R5 HIV-1 infection Patricia Rueda, Javier Garcia-Perez, Isabelle Staropoli, Esther Kellenberger, Jose Alcami, Fernando Arenzana-Seisdedos, Bernard Lagane From 16 International Symposium on HIV and Emerging Infectious Diseases Marseille, France. 24-26 March 2010
NMR experiments on ketals VI. PMR spectra of alkyl substituted 1, 3‐dioxanes with rigid or anancomeric structures (1966)

Abstract PMR coupling values and shifts of twenty three 1, 3‐dioxanes with rigid structure have been determined. The coupling data are in accordance with a chair conformation which is less buckled than that of cyclohexane derivatives. It is necessary to take into account the recently recognized influence of free electron pair orbitals on gem and vicinal coupling phenomena ( 11 ). An exceptional situation for the equatorial proton on C 5 is found, not only for the couplings of this proton, but also for its shift. An influence caused by the shielding of the oxygen atoms (O 1 and O 3 ), their (equatorial) p ‐orbitals overlapping with the C‐H orbital, has been shown to be responsible for coupling value variations.
Designing an intelligent user interface for instructional video indexing and browsing (2006)

Instructional videos are used intensively in universities for remote education and e-learning, and a typical university course consists of videos of more than two thousand minutes in total length. This paper presents a novel graphics user interface for indexing and browsing such extensive but thematically related content. We present how the interface automatically extracts semantic indices from the visual content, and then presents both high- and low-level cues from five different conceptual viewpoints. We detail each of these novel UI units, and show how they are integrated into a user-adjustable main framework, and interconnected and navigated through user mouse events.
ChemInform Abstract: Cycloaromatization Reactions of Methyl 4‐Carboxymethyl‐5‐methoxypenta‐ 2,4‐dienoate. (1988)

Abstract Coumalic acid (I) is cleaved upon prolonged treatment with methanol (II) in the presence of sulfuric acid and trimethyl orthoformate to produce the methoxybutadienedicarboxylate (III), together with minor amounts of the dimethoxy derivative (IV).
Cognitive Function and the Risk for Diabetes Among Young Men (2014)

This study demonstrates that in addition to a potential causal link between diabetes and enhanced cognitive decline, lower cognitive function at late adolescence is independently associated with an elevated risk for future diabetes.
Exoglycosidase Sequencing of N-linked Glycans by the Reagent Array Analysis Method (RAAM) (2003)

In comparison to the sequencing of other biological macromolecules such as nucleic acids or proteins, the sequencing of oligosaccharides (glycans) of glycoproteins generally requires specialized expertise and facilities Whereas techniques such as nuclear magnetic resonance can give full sequence information (, , ), they are only available in relatively few laboratories and are by no means routine (discussed further in Chapter 11).
Heterokont Predator Develorapax marinus gen. et sp. nov. – A Model of the Ochrophyte Ancestor (2016)

Heterotrophic lineages of Heterokonta (or stramenopiles), in contrast to a single monophyletic group of autotrophs, Ochrophyta, form several clades that independently branch off the heterokont stem lineage. The nearest neighbors of Ochrophyta in the phylogenetic tree appear to be almost exclusively bacterivorous, whereas the hypothesis of plastid acquisition by the ancestors of the ochrophyte lineage suggests an ability to engulf eukaryotic alga. In line with this hypothesis, the heterotrophic predator at the base of the ochrophyte lineage may be regarded as a model for the ochrophyte ancestor. Here, we present a new genus and species of marine free-living heterotrophic heterokont Develorapax marinus, which falls into an isolated heterokont cluster, along with the marine flagellate Developayella elegans, and is able to engulf eukaryotic cells. Together with environmental sequences D. marinus and D. elegans form a class-level clade Developea nom. nov. represented by species adapted to different environmental conditions and with a wide geographical distribution. The position of Developea among Heterokonta in large-scale phylogenetic tree is discussed. We propose that members of the Developea clade represent a model for transition from bacterivory to a predatory feeding mode by selection for larger prey. Presumably, such transition in the grazing strategy is possible in the presence of bacterial biofilms or aggregates expected in eutrophic environment, and has likely occurred in the ochrophyte ancestor.
Mode of action of chemical additives in enhancing the potency of<i>Bacillus thuringiensis</i>against lepidopterous insects (1992)

Abstract Ultra‐sonication of Dipel 2X (R) suspension ( Bacillus thuringiensis var. kurstaki HD‐1) followed by combining it with non‐toxic chemical additives or exposure to dynamic magnetic field and its enhancing effect on the potency against Spodoptera littoralis has been investigated. Sonication coupled with or without chemical additives led to an increase in the endotoxin protein solubility, as well as sulphahydryl content, enthalpy and entropy. The existence of the endotoxin protein in the form of trimer, dimer and monomer was demonstrated by chromatography. The cleavage of dimer and monomer from the trimer protein occurred more obviously, when guanidine sulphate was added after sonication. A similar mode of action occurred when sonication of B. thuringiensis was followed by exposure to dynamic magnetic field. Zusammenfassung Über den Einfluß chemischer Zusatzstoffe zwecks Steigerung der Wirkung von Bacillus thuringiensis gegen Lepidopterenlarven Es wurde der Einfluß einer Ultraschallbehandlung einer Dipel 2 X (R)‐Suspension ( Bacillus thuringiensis var. kurstaki HD‐1) in Kombination mit einer nicht toxischen Zusatzsubstanz oder der Einwirkung eines magnetischen Feldes hinsichtlich einer Steigerung der Bacillus thuringiensis ‐Wirkung auf Spodoptera littoralis ‐Larven untersucht. Die Ultraschallbehandlung führte mit oder ohne chemische Zusatzstoffe zu einer Erhöhung sowohl der Löslichkeit des Endotoxin‐Proteins als auch des Sulfahydrilgehalts, der Enthalpie und Entropie. Die Existenz von Endotoxin‐Protein trimerer, dimerer und monomerer Form wurde chromatografisch nachgewiesen. Die Abspaltung von dimerem und monomerem Protein von trimerem Protein war augenfälliger, wenn Guanidinsulfat nach der Ultraschallbehandlung beigefügt wurde. Ein ähnlicher Wirkungsmodus trat ein, wenn auf die Ultraschallbehandlung von Bacillus thuringiensis die Einwirkung eines dynamischen magnetischen Feldes folgte.
Hybrid Ion-Detector/Data-Acquisition System for a TOF-MS (2006)

A modified ion-detector/data-acquisition system has been devised to increase the dynamic range of a time-of-flight mass spectrometer (TOF-MS) that, previously, included a microchannel-plate detector and a data-acquisition system based on counting pulses and time-tagging them by use of a time-to-digital converter (TDC). The dynamic range of the TOF-MS was limited by saturation of the microchannel plate detector, which can handle no more than a few million counts per second. The modified system includes (1) a combined microchannel plate/discrete ion multiplier and (2) a hybrid data-acquisition system that simultaneously performs analog current or voltage measurements and multianode single-ion-pulse-counting time-of-flight measurements to extend the dynamic range of a TDC into the regime in which a mass peak comprises multiple ions arriving simultaneously at the detector. The multianode data are used to determine, in real time, whether the detector is saturated. When saturation is detected, the data-acquisition system selectively enables circuitry that simultaneously determines the ion-peak intensity by measuring the time profile of the analog current or voltage detector-output signal.
The Prodomain-bound Form of Bone Morphogenetic Protein 10 Is Biologically Active on Endothelial Cells (2015)

BMP10 is highly expressed in the developing heart and plays essential roles in cardiogenesis. BMP10 deletion in mice results in embryonic lethality because of impaired cardiac development. In adults, BMP10 expression is restricted to the right atrium, though ventricular hypertrophy is accompanied by increased BMP10 expression in a rat hypertension model. However, reports of BMP10 activity in the circulation are inconclusive. In particular, it is not known whether in vivo secreted BMP10 is active or whether additional factors are required to achieve its bioactivity. It has been shown that high-affinity binding of the BMP10 prodomain to the mature ligand inhibits BMP10 signaling activity in C2C12 cells, and it was proposed that prodomain-bound BMP10 (pBMP10) complex is latent. In this study, we demonstrated that the BMP10 prodomain did not inhibit BMP10 signaling activity in multiple endothelial cells, and that recombinant human pBMP10 complex, expressed in mammalian cells and purified under native conditions, was fully active. In addition, both BMP10 in human plasma and BMP10 secreted from the mouse right atrium were fully active. Finally, we confirmed that active BMP10 secreted from mouse right atrium was in the prodomain-bound form. Our data suggest that circulating BMP10 in adults is fully active and that the reported vascular quiescence function of BMP10 in vivo is due to the direct activity of pBMP10 and does not require an additional activation step. Moreover, being an active ligand, recombinant pBMP10 may have therapeutic potential as an endothelial-selective BMP ligand, in conditions characterized by loss of BMP9/10 signaling.