gebhart/patrepeval huggingface dataset:
1,3-beta-Glucan Synthase: A Useful Target for Antifungal Drugs (2001)

1,3-beta-glucan synthase, a multisubunit enzyme, is responsible for fungal cell wall construction, division septum deposition, and ascospore wall assembly. The catalytic subunit of this enzyme complex, an integral membrane protein, has been identified both in model yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe, and in pathogenic fungi such as Candida, Aspergillus, Cryptococcus and Pneumocystis species. The catalytic activity of the 1,3-beta-glucan synthase is regulated by a small GTPase of the Ras superfamily, the Rho-GTPase, and protein kinase C (Pkc)-like signaling molecules. It has been shown that the plasma membrane localization of this enzyme is essential for its activity. Interestingly, inhibition of 1,3-beta-glucan synthase activity by anti-fungal drugs of the lipopeptide type triggers a cell cycle feedback mechanism leading to cell cycle arrest. Recent progress in studies of molecular mechanisms of the temporal and spatial regulation of 1,3-beta-glucan synthase is presented. The implication of the cell cycle checkpoint that is activated by the anti-fungal drugs is also discussed.
Synthesis, Antibacterial and Antifungal Activity of 4-Substituted-5-Aryl-1,2,4-Triazoles (2001)

A few 4-allyl/amino-5-aryl-1,2,4-triazoles were synthesized and tested for antibacterial and antifungal effects against Escherichia coli, Bacillus subtilis, Salmonella enteritidis, Staphylococcus aureus, Aspergillus niger and Candida albicans. 4-Allyl-5-aryl-1,2,4-triazoles were obtained by the oxidative cyclization of the appropriate 1-substituted-4-allylthiosemicarbazides and 4-amino-5-aryl-1,2,4-triazoles were obtained by cyclization of the potassium salts of appropriately substituted dithiocarbazinic acids with hydrazine hydrate. The new synthesized compounds were characterized using IR, 1H- NMR, 13C-NMR and UV spectral data together with elemental analysis.
Slip detection and control using tactile and force sensors (2000)

In this paper, some results concerning the detection and control of the relative motion (slippage) of two bodies in contact are presented. The main motivation of this research can be found in advanced manipulation with robotic systems, in which, depending on the particular task to be executed, it might be desirable either to avoid or to exploit the slippage of the manipulated object. The main contribution of this paper is that, besides the linear Coulomb friction effect, the rotational case is also addressed and, therefore, both translational and rotational motions are taken into account. This result is achieved by using an integrated sensor constituted by a force/torque and a tactile sensor. Experimental results are presented considering both the detection of the slippage and its subsequent control.
Reducing Unemployment in Nigeria- an Evaluation of the Entrepreneurship Programmers in Ondo State (2018)

The study evaluated the entrepreneurship programme in Ondo State. Descriptive survey research design was adopted for the study and was conducted in Ondo State. Three research questions guided the study. The target population of the study comprised individuals within three local governments: Akoko North East, Akure North and Okitipupa, in Ondo State, Nigeria. Stratified sampling technique was used within the three local governments which differ by their languages. Thereafter, while using a purposive sampling technique, a total number of five hundred (500) respondents were engaged in the study. Respondents were chosen from Akoko North-East, Akure North and Okitipupa. The number of respondents in each of the sampled local government include: Akoko North East (200) respondents, Akure North (150) respondents; and Okitipupa (150) respondents. In total, 264 men and 209 women were engaged. The researchers' developed instruments titled ‘‘Evaluation of Entrepreneurship Programme Questionnaire’’ (EEPQ) was used for data collection. The face validation of the instrument was established by three experts, two in the Department of Guidance and Counselling (Assessment and Testing Unit), University of Ibadan and one in the Department of Educational Management and Policy, Nnamdi Azikiwe University, Awka. Test retest was used to determine the reliability of the instrument and the overall scale was conducted for the interval of three weeks. The reliability coefficient of 0.78 was obtained. The researchers with the help of the community heads in each of the town collected data for the study . Out of the five hundred questionnaires administered, four hundred and seventy three (473) were returned in good state and were therefore subjected to analysis. Data collected on the study were analyzed using frequency count, percentage, and mean. The findings of the study revealed among others that respondents were aware of the government and non-governmental entrepreneurship programs for poverty reduction. The study concluded among others that Ondo State people do not possess adequate entrepreneurial skills for reducing unemployment. Recommendations were made.
Cyclo(<scp>L</scp>-leucyl-α,β-dehydrophenylalanine): the first diketopiperazine containing an α,β-dehydrophenylalanine residue (2005)

The title compound (systematic name: 3-benzylidene-6-isobutylpiperazine-2,5-dione), C15H18N2O2, an alpha,beta-dehydrophenylalanine containing diketopiperazine, crystallizes in the space group P1 with two molecules in the asymmetric unit arranged antiparallel to one another. The alpha,beta-dehydrophenylalanine (DeltaPhe) residue in this cyclic peptide retains its planarity but deviates from the standard conformations observed in its linear analogues. Each type of molecule forms a linear chain with molecules of the same type via pairwise N-H...O hydrogen bonds, while weaker C-H...O interactions link the chains together to form a three-dimensional network.
ChemInform Abstract: CHEMISTRY OF OPIUM ALKALOIDS. PART XVI. SYNTHESIS OF PEPTIDE‐MORPHINANS BASED D ON DIELS‐ALDER ADDUCTS OF THEBAINE WITH ENKEPHALIN MOIETIES (1983)

Abstract DasDiels‐Alder‐Produkt (IIIa) aus den Komponenten (I) [(‐)‐Thebain] und (II) wird verseift [‐ (IIIb)] und durch Umsetzung des Säurechlorids (IIIc) mit den Estern (IV), C‐terminalen Peptidsegmenten des endogenen Opiats Leu‐Enkephalin, in die Verbindungen (V) übergeführt.
Comparison of Trace Metal Concentrations in Malign and Benign Human Prostate (2004)

Imbalance in the composition of trace metals, recognized to be essential to normal human homeostasis, besides the accumulation of potentially toxic or nonessential trace metals, may cause disease. Thus, there is a need for their analysis in cancerous and noncancerous human tissues to examine the relationship between cancer and these elements. Trace metal concentrations including Cd, Ni, Zn, Cu, Fe, Mg, and Ca in both malign and benign prostate samples were determined by atomic absorption spectrophotometry. The tissues were digested by using microwave energy. In contrast to the literature data for zinc, the concentrations of calcium and zinc in the malign human prostate were found to be significantly higher than those in the benign human prostate (p < 0.05 for both metals). Similarly, the concentrations of iron, nickel, and magnesium in the malign prostate were also found to be higher than those in the benign prostate (p </= 0.1). Therefore, it is understood that more studies are needed regarding the increase or decrease in the metal (particularly Ca and Zn) concentrations of malign prostate samples.
Minimization of data address computation overhead in DSP programs (2002)

Digital signal processors (DSPs) provide dedicated data address generation units (AGUs) with multiple register files. These units allow data memory access by indirect addressing with automatic address modification. Typically, both linear and modulo addressing are supported. There is no address computation overhead if the next address is within the auto-modify range. Often, this range can be adapted to the application by assigning static values to modify registers. We discuss optimized data memory address generation in DSP programs. Here the goal is to minimize data address computation and register initialization costs by optimizing data memory layout, address register assignment, and auto-modify range. The investigated combinatorial optimization problems can have an extremely large solution space. However, experimental results indicate that random neighbourhood sampling by simulated annealing allows one to produce highly optimized solutions.
Lauroyl peroxides as polymer catalysts and other uses (1980)

Lauroyl peroxide can be produced by reaction of lauroyl chloride with hydrogen alkali metal peroxides, e.g. it can be produced by treatment of lauroyl chloride with hydrogen peroxide and potassium hydroxide. It has also been prepared by reacting a mixture of lauroyl chloride, lauric anhydride and phosphorous trichloride in benzene, with a mixture of hydrogen peroxide, water, sodium phosphate and caustic soda. It can be dried in the form of an emulsion by centrifuging.
Nutrient Intake Status, Knowledge,Source of Information and Self-perceived Health Status Among Older Adults in South Carolina (1989)

Data on 339 subjects who were over 51 years of age was analyzed and Pearson product variant correlation coefficients were estimated between the variables of nutrient intake, nutrition, knowledge, source of information and self perceived health status. Nutrient status was based on the consumption of eight nutrients (protein, calcium, iron, vitamin A, vitamin C, niacin, riboflavin and thiamin). A significant positive relationship was found between nutrition status and nutrition knowledge, but no significant relationships were found between self-perceived health status and nutrition status or between self- erceived health status and nutrition knowledge. A significant Diffrence was found between sources of nutrition knowledge physicians, dietitians, nurses, etc.) and nutrition status usin the chi-square statistic. There was no significant difference on a chi-suare test of sources of nutrition information and self-perceived health status or on self-perceived health status and nutrition status. A stepwise multivariate approach was used to estimate the amount of variance contributed by the independent variables of nutrition knowledge and sources of nutrition information on the dependent variable of nutrition status.
Dual-electrode CMUT with non-uniform membranes for high electromechanical coupling coefficient and high bandwidth operation (2009)

In this paper, we report measurement results on dual-electrode CMUT demonstrating electromechanical coupling coefficient (k(2)) of 0.82 at 90% of collapse voltage as well as 136% 3 dB one-way fractional bandwidth at the transducer surface around the design frequency of 8 MHz. These results are within 5% of the predictions of the finite element simulations. The large bandwidth is achieved mainly by utilizing a non-uniform membrane, introducing center mass to the design, whereas the dual-electrode structure provides high coupling coefficient in a large dc bias range without collapsing the membrane. In addition, the non-uniform membrane structure improves the transmit sensitivity of the dual-electrode CMUT by about 2dB as compared with a dual electrode CMUT with uniform membrane.
Isolation of Bacterium tularense from the sputum of an atypical case of human tularemia (1944)

Abstract This report describes the isolation of B. tularense from the sputum of a child with a nonfatal case of tularemia. The patient was a twelve-year-old girl, who was admitted to the hospital because of unexplained fever. B. tularense was isolated by mouse inoculation on three occasions from the seventeenth to the thirtieth day after the onset of the disease. The identity of the organism was proved by culture and cross agglutination tests with known strains of B. tularense . The last two isolations were made after the patient was discharged from the hospital and had resumed normal activity. The persistence of B. tularense in the respiratory tract indicates that there is a potential danger of contact infection. The white mouse is recommended as a suitable laboratory animal for diagnostic inoculation studies in suspected cases of tularemia.
Sample Preparation Procedure for Cellular Fungi (2008)

A crucial step in quantitative proteomics is an artefact free and reproducible sample preparation protocol, which has to be adapted and optimized to nearly all types of cells. Here we provide a sample preparation method for quantitative proteomics of cellular fungi. Two different protein extraction methods were compared with focus on reproducibility, minimized proteolytic degradation and protein losses during the sample preparation. In the first preparation the cells were lysed by sonication followed by protein solubilization in "standard" lysis buffer. The second preparation was performed with a SDS-presolubilization step followed by sonication and further boiling, before diluting the sample with lysis buffer. We have shown that the sample preparation for cellular fungi is performed with maximum protein solubilization, higher reproducibility and a reduced proteolytic activity by including a SDS-presolubilization step in the sample preparation protocol.
STRATEGIES TOWARD CNS-REGENERATION USING INDUCED PLURIPOTENT STEM CELLS (2009)

Induced pluripotent stem (iPS) cells are pluripotent stem cells directly reprogrammed from cultured mouse fibroblast by introducing Oct3/4, Sox2, c-Myc, and Klf4. Cells obtained using this technology, which allows the ethical issues and immunological rejection associated with embryonic stem (ES) cells to be avoided, might be a clinically useful source for cell replacement therapics. Here we demonstrate that murine iPS cells formed neurospheres that produced electrophysiologically functional neurons, astrocytes, and oligodendrocytes. Secondary neurospheres (SNSs) generated from various mouse iPS cell showed their neural differentiation capacity and teratoma formation after transplantation into the brain of immunodeficient NOD/SCID mice. We found that origin (source of somatic cells) of the iPS cells are the crucial determinant for the potential tumorigenicity of iPS-derived neural stem/progenitor cclls and that their tumorigenicity results from the persistent presence of undifferentiated cells within the SNSs. Furthermore, transplantation of non-tumorigenic Nanog-iPS-derived SNSs into mouse spinal cord injury (SCI) model promoted locomotor function recovery. Surprisingly, SNSs derived from c-Myc minus iPS cells generated without drug selection showed robust tumorigenesis, in spite of their potential to contribute adult chimeric mice without tumor formation.
Immunoglobulin expression by human B lymphocytes clonally transformed by Epstein Barr virus. (1982)

Epstein Barr virus (EBV) immortalizes human B lymphocytes, resulting in long-term lymphoblastoid cell lines. To date, such cell lines have typically been derived by suspension culture of mixed mononuclear cells, infected in vivo or in vitro with EBV. We have derived 208 lymphoblastoid lines by clonal transformation of peripheral blood lymphocytes from 3 neonates, 3 normal adults, and 1 patient with systemic lupus erythematosus (SLE), utilizing colony formation in agarose and then separate propagation of the colonies in liquid medium. A total of 180 lines have been analyzed for expression of cytoplasmic and/or secreted immunoglobulin isotypes. Such analysis reveals that 167 (92%) of the lines are monoclonal with respect to isotype expression. All clonally derived lines from neonates produce only IgM. Most clonal transformants from normal adults also produce IgM (81%), but clones producing IgG (16%) or IgA (3%) were also found. Clonal transformation of lymphocytes from the SLE patient revealed a shift in isotype expression, with increased numbers of IgG-secreting clones (48%) and IgA-secreting clones (13%). The data support the use of EBV-mediated clonal transformation as a probe for the analysis of states of isotype differentiation among circulating B cells, and as a method for deriving immortalized, monoclonal human B cell lines.
Nach dem 11. September 2001. Die öffentliche Auseinandersetzung um den Terrorismus und den Afghanistan-Krieg: Opportunismus, Propaganda und Einschüchterung statt menschenrechtsbestimmter argumentativer Diskussion (2017)

Bei den im Folgenden formulierten Überlegungen handelt es sich um eine leicht überarbeitete Version einer Stellungnahme, zu der ich mich unmittelbar nach Beginn des Afghanistan-Krieges bewogen sah. Wegen der grundsätzlichen Form der Argumentation hat sie meines Erachtens jedoch nichts an Relevanz verloren. An Aktualität hat sie sogar gewonnen. Der — von mir schon damals befürchtete — Irak-Krieg ist tatsächlich geführt und die Verletzung der Menschenrechte im Namen des tagtäglich verkündeten "...
Rapid Identification of Bacteria in Blood Cultures by Using Fluorescently Labeled Oligonucleotide Probes (2000)

The applicability of whole-cell hybridization for the identification of pathogenic bacteria in blood from septic patients was examined. Oligonucleotide probes, fluorescently labeled with fluorescein isothiocyanate, directed against the variable regions of the 16S rRNAs of the following bacterial species and/or genera were used: Streptococcus spp., Enterococcus faecalis, Staphylococcus aureus, coagulase-negative staphylococci (CoNS), Escherichia coli, Pseudomonas aeruginosa, and the Enterobacteriaceae family. A probe specific for the rRNAs of almost all bacteria and its complementary, reversed counterpart was used as positive and negative control, respectively. The probes were used in conjunction with a fast and simple-to-use protocol for whole-cell hybridization. This protocol yields an identification after 25 to 45 min, depending on whether the bacterium is gram positive or gram negative. A total of 182 blood samples which tested positive in a blood culture machine were investigated. All probes except for the ones for S. aureus and the CoNS showed sensitivities and specificities of 1.000. It was concluded that whole-cell hybridization is well suited for the fast screening of septic blood containing streptococci and/or enterococci or gram-negative rods.
The market for hotels: if they come will you build it? (1998)

New England; Real property
An evolutionary and structure‐based docking model for glucocerebrosidase–saposin C and glucocerebrosidase–substrate interactions—Relevance for Gaucher disease (2007)

Gaucher disease, the most prevalent lysosomal storage disorder, is principally caused by malfunction of the lysosomal enzyme glucocerebrosidase (GBA), a 497-amino acid membrane glycoprotein that catalyzes the hydrolysis of glucosylceramide to ceramide and glucose in the presence of an essential 84-residue activator peptide named saposin C (SapC). Knowledge of the GBA structure, a typical (beta/alpha)(8) TIM barrel, explains the effect of few mutations, directly affecting or located near the catalytic site. To identify new regions crucial for proper GBA functionality, we analyzed the interactions of the enzyme with a second (substrate) and a third (cofactor) partner. We build 3D docking models of the GBA-SapC and the GBA-ceramide interactions, by means of methodologies that integrate both evolutive and structural information. The GBA-SapC docking model confirm the implication of three spatially closed regions of the GBA surface (TIM barrel-helix 6 and helix 7, and the Ig-like domain) in binding the SapC molecule. This model provides new basis to understand the pathogenicity of several mutations, such as the prevalent Leu444Pro, and the additive effect of Glu326Lys in the double mutant Glu326Lys-Leu444Pro. Overall, 39 positions in which amino acid changes are known to cause Gaucher disease were localized in the GBA regions identified in this work. Our model is discussed in relation to the phenotype (pathogenic effect) of these mutations, as well as to the enzymatic activity of the recombinant proteins when available. Both data fully correlates with the proposed model, which will provide a new tool to better understand Gaucher disease and to design new therapy strategies.
Use of laminar air flow portable filter in asthmatic children. (1977)

A new, portable laminar air-flow filter (Enviracaire) was tested on 13 asthmatic children, using a double-blind single crossover study. There was a statistical improvement in symptom scores during filter use; however, peak flows did not improve significantly. The authors feel this method of avoiding indoor airborne irritants is useful and warrants further study.
Microwave Resonant Absorption of Potential Exothermic Compounds (1989)

Preventive influenza vaccines must be reformulated annually because of antigen shift and drift of circulating influenza viral strains. However, seasonal vaccines do not always match the circulating strains, and there is the ever-present threat that avian influenza viruses may adapt to humans. Hence, a universal influenza vaccine is needed to provide protective immunity against a broad range of influenza viruses. We designed an influenza antigen consisting of three tandem M2e repeats plus HA2, in combination with a detoxified anthrax oedema toxin delivery system (EFn plus PA) to enhance immune responses. The EFn-3×M2e-HA2 plus PA vaccine formulation elicited robust, antigen-specific, IgG responses; and was protective against heterologous influenza viral challenge when intranasally delivered to mice three times. Moreover, use of the detoxified anthrax toxin system as an adjuvant had the additional benefit of generating protective immunity against anthrax. Hence, this novel vaccine strategy could potentially address two major emerging public health and biodefence threats.
A grid-enabled Web map server (2005)

Today Geographic Information Systems (GIS) provide several tools for studying and analyzing varied human and natural phenomena, therefore GIS and geospatial data has grown so much in both public and private organizations. A challenge is the integration of these data to get innovative and exhaustive knowledge about topics of interest. In this paper we describe the design of a Web map service (WMS) OGC-compliant, through the use of grid computing technology and demonstrate how this approach can improve, w.r.t. security, performance, efficiency and scalability, the integration of geospatial multi-source data. End users, with a single sign-on, securely and transparently, gets maps whose data are distributed on heterogeneous data sources belonging to one or more Virtual Organizations via distributed queries in a grid computing environment.
Simulation of Fluid Flow in Centrifugal Tricanter (2013)

Abstract An ANSYS simulation of the multiphase complex fluid flow motion in a centrifugal device (tricanter) is presented in the paper. This centrifugal device is designed for one step efficient solution for contaminated river water processing with oil and oil products. The proposed tricanter is one of the main objectives of the project named “Common strategy to prevent the Danube’s pollution technological risks with oil and oil products CLEANDANUBE” financed by European Commission within the frame of Romania-Bulgaria Trans-Border Cooperation Program 2007 - 2013 (grant MIS-ETC code 653). Results for liquid phases (water and oil products) and for solid particles motion are presented graphically and are commented.
A Parametric Model Approach to Arc Fault Detection for DC and AC Power Systems (2006)

Arc faults can arise in any electrical system as a consequence of loose connections, inadvertent damage during maintenance and/or insulation aging. In this paper, a new approach to the detection of arc faults is discussed. By using the proposed parametric models of the fault signals, an arc fault can be identified quickly and reliably, so that action can be taken to prevent the arc from escalating into a serious fire and causing loss of life and/or property. One model was obtained for DC power systems and another model was obtained for AC power systems. These proposed models were able to distinguish arc faults from normal operation of the various electrical loads that were tested