gebhart/patrepeval huggingface dataset:
Object-oriented data handling system for an automated chemistry laboratory (1991)

Our results indicate that there is no need to administer enoxaparin over two injections for ENHD up to 8 h. The usefulness of monitoring anti-Xa levels to predict fiber patency, assessed by µCT, can be questioned, but further clinical trials are needed.
The O Antigen Is a Critical Antigen for the Development of a Protective Immune Response to <i>Bordetella parapertussis</i> (2009)

Despite excellent vaccine coverage in developed countries, whooping cough is a reemerging disease that can be caused by two closely related pathogens, Bordetella pertussis and B. parapertussis. The two are antigenically distinct, and current vaccines, containing only B. pertussis-derived antigens, confer efficient protection against B. pertussis but not against B. parapertussis. B. pertussis does not express the O antigen, while B. parapertussis retains it as a dominant surface antigen. Since the O antigen is a protective antigen for many pathogenic bacteria, we examined whether this factor is a potential protective antigen for B. parapertussis. In a mouse model of infection, immunization with wild-type B. parapertussis elicited a strong antibody response to the O antigen and conferred efficient protection against a subsequent B. parapertussis challenge. However, immunization with an isogenic mutant lacking the O antigen, B. parapertussis Deltawbm, induced antibodies that recognized other antigens but did not efficiently mediate opsonophagocytosis of B. parapertussis. The passive transfer of sera raised against B. parapertussis, but not B. parapertussis Deltawbm, reduced B. parapertussis loads in the lower respiratory tracts of mice. The addition of 10 microg of purified B. parapertussis lipopolysaccharide (LPS), which contains the O antigen, but not B. parapertussis Deltawbm LPS drastically improved the efficacy of the acellular vaccine Adacel against B. parapertussis. These data suggest that the O antigen is a critical protective antigen of B. parapertussis and its inclusion can substantially improve whooping cough vaccine efficacy against this pathogen.
Prospective monitoring of <i>BCR‐ABL1</i> transcript levels in patients with Philadelphia chromosome‐positive acute lymphoblastic leukaemia undergoing imatinib‐combined chemotherapy (2008)

The clinical significance of minimal residual disease (MRD) is uncertain in patients with Philadelphia chromosome-positive acute lymphoblastic leukaemia (Ph+ ALL) treated with imatinib-combined chemotherapy. Here we report the results of prospective MRD monitoring in 100 adult patients. Three hundred and sixty-seven follow-up bone marrow samples, collected at predefined time points during a uniform treatment protocol, were analysed for BCR-ABL1 transcripts by quantitative reverse transcription polymerase chain reaction. Ninety-seven patients (97%) achieved complete remission (CR), and the relapse-free survival (RFS) rate was 46% at 3 years. Negative MRD at the end of induction therapy was not associated with longer RFS or a lower relapse rate (P = 0.800 and P = 0.964 respectively). Twenty-nine patients showed MRD elevation during haematological CR. Of these, 10 of the 16 who had undergone allogeneic haematopoietic stem cell transplantation (HSCT) in first CR were alive without relapse at a median of 2.9 years after transplantation, whereas 12 of the 13 who had not undergone allogeneic HSCT experienced a relapse. These results demonstrate that, in Ph+ ALL patients treated with imatinib-combined chemotherapy, rapid molecular response is not associated with a favourable prognosis, and that a single observation of elevated MRD is predictive of subsequent relapse, but allogeneic HSCT can override its adverse effect.
Line differences in resistance to Salmonella enteritidis PT4 infection (1996)

International audience
Biochemical Characterization and Mechanistic Analysis of the Levoglucosan Kinase from <i>Lipomyces starkeyi</i> (2017)

Levoglucosan kinase (LGK) catalyzes the simultaneous hydrolysis and phosphorylation of levoglucosan (1,6-anhydro-β-d-glucopyranose) in the presence of Mg<sup>2+</sup> -ATP. For the Lipomyces starkeyi LGK, we show here with real-time in situ NMR spectroscopy at 10 °C and pH 7.0 that the enzymatic reaction proceeds with inversion of anomeric stereochemistry, resulting in the formation of α-d-glucose-6-phosphate in a manner reminiscent of an inverting β-glycoside hydrolase. Kinetic characterization revealed the Mg<sup>2+</sup> concentration for optimum activity (20-50 mm), the apparent binding of levoglucosan (K<sub>m</sub> =180 mm) and ATP (K<sub>m</sub> =1.0 mm), as well as the inhibition by ADP (K<sub>i</sub> =0.45 mm) and d-glucose-6-phosphate (IC<sub>50</sub> =56 mm). The enzyme was highly specific for levoglucosan and exhibited weak ATPase activity in the absence of substrate. The equilibrium conversion of levoglucosan and ATP lay far on the product side, and no enzymatic back reaction from d-glucose-6-phosphate and ADP was observed under a broad range of conditions. 6-Phospho-α-d-glucopyranosyl fluoride and 6-phospho-1,5-anhydro-2-deoxy-d-arabino-hex-1-enitol (6-phospho-d-glucal) were synthesized as probes for the enzymatic mechanism but proved inactive with the enzyme in the presence of ADP. The pyranose ring flip <sup>4</sup> C<sub>1</sub> →<sup>1</sup> C<sub>4</sub> required for 1,6-anhydro-product synthesis from d-glucose-6-phosphate probably presents a major thermodynamic restriction to the back reaction of the enzyme.
The High mobility group box 1/Toll-like Receptor 2 axis plays a non-inflammatory role in the self-renewal of mammary cancer stem cells (2014)

The lack of effective treatment in several metastatic cancers raises the question of whether current therapies target the right cells. These treatments may be missing cancer stem cells (CSC), a sub-population of stem cell-like cells that are able to play a critical role in cancer progression. A profound understanding of the mechanisms that regulate CSC selfrenewal is therefore essential for the identification of new CSC antigens that may turn out to be the ideal target for more effective anticancer strategies. In the light of these considerations, we have performed the transcription profiling of the murine ErbB2+ breast tumor cell line TUBO versus its derived CSC-enriched mammospheres. Of the all antigens that have been identified, we have focused our attention on toll-like receptor (TLR)2 which has been identified as being overexpressed in CSC. Moreover, TLR2 plays a central role in CSC biology; it is a key molecule for CSC selfrenewal as its signaling inhibition impairs in vitro mammosphere generation and blocks tumorigenesis and lung metastases development in vivo. Our in-depth analysis of the downstream signal driven by TLR2 has demonstrated that it is activated by endogenous high mobility-group box (HMGB)1 with an autocrine loop, which induces IBα phosphorylation, IL-6 and TGFβ secretion and consequently STAT3 and Smad3 activation.
Multi-Layer Simulation Design and Validation for a Two-Tier Fault-Tolerant WDM LAN (2012)

In this paper, we report our progress on the development of a simulation framework for optical local area networks, which enables a multi-layer network simulation and hence bridges the gap between the traditional signal-level and traffic-level simulators. The framework, called DRAGON (discrete-model register for Artifex-based general-purpose optical local area network simulation), is built upon the Artifex modeling platform, and it models a wide register of fiber-optic components, emulating features at multiple network layers, spanning over signal and noise power monitoring, bit error rate (BER) estimation, packet collision detection, and propagation/transmission delay emulation. We demonstrate the effectiveness of DRAGON by modeling a novel two-tier fault-tolerant wavelength division multiplexing local area network (WDM LAN) architecture, and we show the impact of the proposed WDM LAN architecture on the quality of service requirements of WDM traffic.
Spin echo magnetic resonance imaging (2013)

The spin echo sequence is a fundamental pulse sequence in MRI. Many of today's applications in routine clinical use are based on this elementary sequence. In this review article, the principles of the spin echo formation are demonstrated on which the generation of the fundamental image contrasts T1, T2, and proton density is based. The basic imaging parameters repetition time (TR) and echo time (TE) and their influence on the image contrast are explained. Important properties such as the behavior in multi-slice imaging or in the presence of flow are depicted and the basic differences with gradient echo imaging are illustrated. The characteristics of the spin echo sequence for different magnetic field strengths with respect to clinical applications are discussed.
The Calvin Cycle Synthesizes Hexoses from Carbon Dioxide and Water (2002)

Nodular pulmonary amyloidosis is a rare and localized manifestation of amyloid deposition in the lungs. This rare entity, though asymptomatic, is often misdiagnosed on imaging alone, due to its resemblance to metastatic pulmonary nodules. This report highlights the significance of histologic confirmation before treatment, as a preventive measure against overtreatment.
Analysis of Complex Permittivity of Liposome for Its Biochemical Dynamics up to 30 GHz Range (2006)

We have successfully measured the complex permittivity of liposome as very important biochemical species, dependent on frequency up to 30 GHz (millimeter-wave range), for the first time, by an open-ended coaxial probe method improved for measuring a very small amount of liquid solution. We used a probe size as small as 1.2 mmphi, which leads to upper frequency limit usable of 90 GHz. Also found that the method is very suitable for evaluation of series of biochemical solution with a small amount (1-2 ml) in a short period. It is revealed, as a result of membrane dynamics of liposome, that 1) temperature dependency of relaxation time of DPPC liposome in dispersion regions (0.2-30 GHz), is attributed to bulk water, cation and anion, 2) increase in the relaxation time results from dynamics of trapping/bounding of water by cation and anion, and 3) cholesterol addition to liposome gives a new dispersion (around 1 GHz), possible to relate to water molecules that freely move in/on the liposome surface. We think the method is a promising tool for exploring basic biochemistry of liposome and related solution systems
An Improved Synthesis of Vinylic Phosphonates from Ketones<sup>1</sup> (1980)

Abstract A convenient route for the synthesis of β-substituted vinylphosphonates utilizes the reaction of aldehydes with diphenyl triphenylphosphoranylidenemethylphosphonate.2,3 Unfortunately, this ylide system is unreactive with ketones for the generation of β,β-disubstituted vinylphosphonates. Tetraethyl methylenebisphosphonate carbanion has been used in place of the ylide in reaction with a number of aldehydes,3 but has found only limited use in reaction with ketones.4,5
Development of Hydropersulfide Donors to Study Their Chemical Biology (2021)

<b><i>Significance:</i></b> Hydropersulfides (RSSH) are ubiquitous in prokaryotes, eukaryotic cells, and mammalian tissues. The unique chemical properties and prevalent nature of these species suggest a crucial role of RSSH in cell regulatory processes, yet little is known about their physiological functions. <b><i>Recent Advances:</i></b> Examining the biological roles of RSSH species is challenging because of their inherent instability. In recent years, researchers have developed a number of small-molecule donors that efficiently release RSSH in response to various stimuli, including pH, thiols, reactive oxygen species, enzymes, and light. These RSSH donors have provided researchers with chemical tools to uncover the potential function and role of RSSH as physiological signaling and/or protecting agents. <b><i>Critical Issues:</i></b> Because RSSH, hydrogen sulfide (H<sub>2</sub>S), and higher order polysulfides are related to each other and can be present simultaneously in biological systems, distinguishing among the activities due to each of these species is difficult. Discerning this activity is critical to elucidate the chemical biology and physiology of RSSH. Moreover, although RSSH donors have been shown to confer cytoprotection against oxidative and electrophilic stress, their biological targets remain to be elucidated. <b><i>Future Directions:</i></b> The development of RSSH donors with optimal drug-like properties and selectivity toward specific tissues/pathologies represents a promising approach. Further investigation of releasing efficiencies <i>in vivo</i> and a clear understanding of RSSH biological responses remain targets for future investigation. <i>Antioxid. Redox Signal</i>. 36, 309-326.
Variability Analysis of MOS Differential Amplifier (2009)

Variation characteristics in MOS differential amplifier are evaluated by using the concise statistical model parameters for SPICE simulation. We find that the variation in the differential-mode gain, Adm, induced by the current factor variation, Δβ0, in the Id-variation of the differential MOS transistors is more than one order of magnitude larger than that induced by the threshold voltage variation, ΔVth, which has been regarded as a major factor for circuit variations in SoC's (2). The results obtained by the Monte Carlo simulations are verified by the theoretical analysis combined with the sensitivity analysis which clarifies the specific device parameter dependences of the variation in Adm.
ChemInform Abstract: Synthesis and Antiviral Activity of 1‐Cyclobutyl‐5‐(2‐bromovinyl)uracil Nucleoside Analogues and Related Compounds. (1993)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Effects of furosemide, piretanide, and water loading on urinary excretion of cefazolin in humans (1984)

Antibiotics and diuretics are often prescribed concomitantly for humans. We compared the effects of two potent loop diuretics, furosemide and piretanide, with those of water loading on the urinary excretion of cefazolin. During a continuous infusion of inulin and cefazolin (10 mg/kg per h), six healthy male volunteers received a single intravenous injection of furosemide (0.3 mg/kg) or piretanide (0.1 mg/kg) or again an oral water load of 15 ml/kg over a 20-min period. In vitro, furosemide at all concentrations tested significantly reduced by about 10% the percentage of cefazolin bound to serum proteins. Piretanide exhibited such an effect only at a concentration of 2 micrograms/ml. Furosemide, piretanide, and water loading significantly and similarly increased the ratio of excreted to infused cefazolin up to 2 h after the injection of diuretic or after oral water intake. In each of the three parts of the experiment, the increase of the urinary flow rate was similar when compared with the control values. Furosemide significantly increased the cefazolin filtered load during the same time. Piretanide significantly enhanced the absolute rate of net cefazolin tubular secretion. Water loading increased the urinary excretion of cefazolin, probably through a reduction in tubular reabsorption. These results suggest that (i) furosemide and piretanide as well as water loading are capable of enhancing renal excretion of cefazolin by different complex mechanisms; (ii) cefazolin undergoes a bidirectional tubular transport; (iii) piretanide might act on the proximal tubule in addition to its main site of action on Henle's loop; and (iv) the effects of both diuretics and of water loading are unlikely to affect in vivo antibiotic activity in humans.
Temperature characterization and modeling of electron and hole mobilities in MOS accumulation layers (2002)

The need to understand and predict MOS accumulation layer mobility has become increasingly important as MOS devices scale to the 100 nm gate length range. Indeed, the accumulation layer in the shallow source/drain extension in MOSFETs is becoming a significant part of the series resistance. Also, the accumulation layer is the major carrier transport layer in accumulation mode SOI MOSFETs and PMOS buried-channel MOSFETs. However, temperature dependent experimental data and accurate models for the accumulation layer mobility are not available. This paper reports for the first time accumulation layer mobility models for both electrons and holes. Also for the first time, we report experimental data for both electrons and holes over the important temperature range of 25-150°C for a range of doping concentrations from 1×10/sup 16/ to 4×10/sup 17/ cm/sup -3/. From the newly developed effective mobility models, compact and computationally efficient local mobility models have also been developed and implemented in the PISCES and MINIMOS 2D device simulators. Using the newly developed accumulation layer mobility models, a more accurate prediction of the device drive current has been made possible for both N-channel and P-channel deep submicron MOSFETs.
A retrospective analysis of integrative medicine consultations at a major cancer center: Reasons for consultation, symptom burden, quality of life, and satisfaction. (2014)

e17630 Background: Many cancer patients use complementary and alternative medicine, but limited data exists regarding the reasons for an integrative medicine consultation and the patient’s symptom burden, quality of life, and satisfaction. Methods: Beginning in September of 2009, all patients receiving an integrative oncology consultation were asked to complete a modified Measure Yourself Concerns and Wellbeing (MYCaW), a modified Edmonton Symptom Assessment Scale (ESAS, 0-10 scale), and a satisfaction rating (0-10 scale). Starting in January of 2013, quality of life was measured by the Patient Reported Outcomes Measurement Information System Global Health (PROMIS-10). Results: A total of 2,564 new patient integrative oncology consultations were conducted from 9/2009 to 12/2013, representing 63% of all consultative visits, with most patients (97.5%, n=2501) completing at least one assessment. About one-quarter of patients (26.0%) had at least one follow-up visit (average 3.1±1.7). The most common reasons for consultation as reported by the MYCaW were integrative approach (32.3%), herbs/supplements (31.9%), nutrition (20.4%), overall health (13.0%), and pain (12.6%). When ranking the importance of the reason for the consultation, 47% marked 10 on a 0-10 points scale for their first reason. Overall satisfaction was rated ≥8 by 94.6% of patients. The highest rated symptoms on the ESAS included poor sleep (mean=4.2), fatigue (4.0), poor memory (3.2), distress (3), and poor appetite (2.8). For the PROMIS-10 (n=711), the standardized T-scores were 43.6 for physical health and 45.8 for mental health (a score of 50 representing healthy population levels). The most common services utilized after the initial consultation were acupuncture (n=1,028) and massage (n = 636). Conclusions: Patients at a major cancer center commonly seek an integrative oncology consultation to address issues of integrative approaches, herbs/supplements and nutrition. Patients had a high satisfaction with the clinical service and reported mild-moderate symptom burden and clinically significant physical health problems.
Machine Knowledge Maniputation Issues in Parallel Compilers (1988)

Parallel compilers need detail architectural knowledge about the target machines to optimize user programs.The knowledge is needed in order to realize the potential parallelism provided by the hardware and to match the program parallelism with the machine parallelism.In most parallel compilers, the architectural infonnation of the target machine are blurred into the control structures of the compilers.Consequently, these parallel compilers are inflexible and substantial efforts are needed to modify them or to port them to different machines.A solution to this problem is to separate the hardware features from the knowledge for the program optimization and describe the later based on these features.In this way, the control of parallel compilers relies explicitly on the computational model that is described by machine features rather than the hard-coded heuristics.High degree of flexibility.portability and knowledge sharing can be achieved among different target machines.
Correction to Letter: Fluorinated Aromatic Amino Acids Are Sensitive <sup>19</sup>F NMR Probes for Bromodomain–Ligand Interactions (2016)

ADVERTISEMENT RETURN TO ISSUEPREVAddition/CorrectionNEXTORIGINAL ARTICLEThis notice is a correctionCorrection to Letter: Fluorinated Aromatic Amino Acids Are Sensitive 19F NMR Probes for Bromodomain–Ligand InteractionsNeeraj K. Mishra, Andrew K. Urick, Stuart W. J. Ember, Ernst Schönbrunn, and William C. Pomerantz*Cite this: ACS Chem. Biol. 2016, 11, 4, 1149Publication Date (Web):February 2, 2016Publication History Published online2 February 2016Published inissue 15 April 2016https://doi.org/10.1021/acschembio.6b00049Copyright © 2016 American Chemical SocietyRIGHTS & PERMISSIONSACS AuthorChoiceArticle Views894Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (596 KB) Get e-Alerts Get e-Alerts
Centrally Administered Insulin-Like Growth Factor II Fails to Alter Pulsatile Growth Hormone Secretion or Food Intake (1992)

Insulin-like growth factor II (IGF-II) peptide, mRNA, and receptors are widely distributed in the central nervous system, yet the physiological role of IGF-II in brain remains largely unknown. In the present study, we examined the in vivo effects of central administration of recombinant human IGF-II on pulsatile GH secretion and food intake. The IGF-II preparation used was shown to stimulate 3H-thymidine incorporation in MG-63 human osteosarcoma cells in vitro. Free-moving adult male rats bearing chronic intracerebroventricular (icv) and intracardiac venous cannulae were icv administered 10 microliters of either IGF-II (in doses of 300 ng and 1 microgram) or the vehicle solution, and blood samples were obtained every 15 min for 6 h. Vehicle-injected control animals exhibited the typical pulsatile pattern of GH secretion with most peak GH values greater than 100 ng/ml and trough levels less than 1.2 ng/ml. Central administration of IGF-II, at both doses, failed to alter the spontaneous 6-hour GH secretory profile; there were no significant differences in either GH peak amplitude, GH trough level, GH interpeak interval, or mean 6-hour plasma GH level, compared to vehicle-injected controls. There was also no effect of icv administered IGF-II on mean plasma glucose or insulin levels. Compared to vehicle-injected control rats, the icv injection of IGF-II (at doses of 300 ng and 1 microgram) did not significantly alter 24-h food intake or body weight gain in normal feeding rats.(ABSTRACT TRUNCATED AT 250 WORDS)