gebhart/patrepeval huggingface dataset:
Electrophoretic Light Scattering, Dynamic Light Scattering, and Turbidimetry Studies of the Effect of Polymer Concentration on Complex Formation between Polyelectrolyte and Oppositely Charged Mixed Micelles (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTElectrophoretic Light Scattering, Dynamic Light Scattering, and Turbidimetry Studies of the Effect of Polymer Concentration on Complex Formation between Polyelectrolyte and Oppositely Charged Mixed MicellesYingjie Li, Paul L. Dubin, Henry A. Havel, Shun L. Edwards, and Herbert DautzenbergCite this: Macromolecules 1995, 28, 9, 3098–3102Publication Date (Print):April 1, 1995Publication History Published online1 May 2002Published inissue 1 April 1995https://pubs.acs.org/doi/10.1021/ma00113a011https://doi.org/10.1021/ma00113a011research-articleACS PublicationsRequest reuse permissionsArticle Views412Altmetric-Citations38LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Faculty Opinions recommendation of prp8 mutations that cause human retinitis pigmentosa lead to a U5 snRNP maturation defect in yeast. (2007)

Prp8 protein (Prp8p) is a highly conserved pre-mRNA splicing factor and a component of spliceosomal U5 small nuclear ribonucleoproteins (snRNPs). Although it is ubiquitously expressed, mutations in the C terminus of human Prp8p cause the retina-specific disease retinitis pigmentosa (RP). The biogenesis of U5 snRNPs is poorly characterized. We present evidence for a cytoplasmic precursor U5 snRNP in yeast that lacks the mature U5 snRNP component Brr2p and depends on a nuclear localization signal in Prp8p for its efficient nuclear import. The association of Brr2p with the U5 snRNP occurs within the nucleus. RP mutations in Prp8p in yeast result in nuclear accumulation of the precursor U5 snRNP, apparently as a consequence of disrupting the interaction of Prp8p with Brr2p. We therefore propose a novel assembly pathway for U5 snRNP complexes that is disrupted by mutations that cause human RP. PMID: 17934474 Funding information This work was supported by: Wellcome Trust, United Kingdom Grant ID: 073988 Wellcome Trust, United Kingdom Grant ID: 067311 Wellcome Trust, United Kingdom Grant ID: 067311
Cross‐subject comparison of principal diffusion direction maps (2005)

Diffusion tensor imaging (DTI) data differ fundamentally from most brain imaging data in that values at each voxel are not scalars but 3 x 3 positive definite matrices also called diffusion tensors. Frequently, investigators simplify the data analysis by reducing the tensor to a scalar, such as fractional anisotropy (FA). New statistical methods are needed for analyzing vector and tensor valued imaging data. A statistical model is proposed for the principal eigenvector of the diffusion tensor based on the bipolar Watson distribution. Methods are presented for computing mean direction and dispersion of a sample of directions and for testing whether two samples of directions (e.g., same voxel across two groups of subjects) have the same mean. False discovery rate theory is used to identify voxels for which the two-sample test is significant. These methods are illustrated in a DTI data set collected to study reading ability. It is shown that comparison of directions reveals differences in gross anatomic structure that are invisible to FA.
Crystallographic Study of Crude Drug Components. II (1950)

Studies were made on the relationship between chemical structure and crystal forms and optical properties of the allied compounds of santonin by the determination of crystallographic data with the use of two-circle goniometer and polarised microscope. Results obtained were as follows:1) Hypothesis of Kariyone and Fukui that monoginin, C15H20O3, had one less double bond than santonin is correct.2) The correctness of Asahina and Ukita's hypothesis regarding temisin and isotemisin was proved by the determination of the various properties of their derivatives.3) Crystallographic data of mibulactone, a new component of Artemisia monogyna, were determined.
Analysis of Hand Tool Use in the Underground Mining Industry (1986)

A research project that is sponsored by the Bureau of Mines, Department of Interior and conducted by Ohio State University is analyzing the accidents caused by hand tools used in the underground coal, metal, and nonmetal mining industries. To describe the accident types and the resulting injuries, a thorough search was conducted of a computer data base of mining-related accidents. After sorting for data pertaining to hand tools, analyses were carried out to determine likely scenarios, and their probability of occurrence, for the various hand tool injuries. This project, which summarized accident data for a six-year period, is the first comprehensive evaluation of hand tool accident data related to underground mining. The method of accident classification, which is based on accident type, part of body injured, and nature of the injury, allows assessment of the key components of the hand tool injury. Furthermore, this analysis, in conjunction with the scenario development, provides direction for future research into hand tool ergonomics.
ChemInform Abstract: HETEROCYCLISCHE SCHWEFELVERBINDUNGEN 67. MITT. SYNTHESE UND REAKTIVITAET VERSCHIEDEN SUBSTITUIERTER ALPHA‐(1,2‐DITHIOLYLIDEN‐(3))‐KETONEN UND 1,6,6A S(IV)‐TRITHIAPENTALENEN (1974)

Abstract Die Kondensation der entsprechenden Dithiolyliumperchlorate bzw. ‐jodide, z,B. t mit den Ketonen (II) liefert in absolutem Alkohol in Abwesenheit basischer Agen; die Ketone (IIIa) und (IIIb) bzw. (IIIc).
Metal complexes of substituted dithiophosphinic acids. VII. Reactions of titanium tetrachloride, vanadium tetrachloride, niobium tetrachloride, niobium pentachloride, and tantalum pentachloride (1972)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMetal complexes of substituted dithiophosphinic acids. VII. Reactions of titanium tetrachloride, vanadium tetrachloride, niobium tetrachloride, niobium pentachloride, and tantalum pentachlorideR. G. Cavell and A. R. SangerCite this: Inorg. Chem. 1972, 11, 9, 2016–2019Publication Date (Print):September 1, 1972Publication History Published online1 May 2002Published inissue 1 September 1972https://pubs.acs.org/doi/10.1021/ic50115a006https://doi.org/10.1021/ic50115a006research-articleACS PublicationsRequest reuse permissionsArticle Views72Altmetric-Citations16LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Characterizing Genetic Circuit Components in <i>E. coli</i> towards a <i>Campylobacter jejuni</i> Biosensor (2018)

Abstract Campylobacter jejuni is responsible for most cases of bacterial gastroenteritis (food poisoning) in the United Kingdom. The most common routes of transmission are by contact with raw poultry. Current detection systems for the pathogen are time-consuming, expensive or inaccessible for everyday users. In this article we propose a cheaper and faster system for detection of C. jejuni using a synthetic biology approach. We aimed to detect C. jejuni by the presence of xylulose, an uncommon bacterial capsular saccharide. We characterized two sugar-based regulatory systems that displayed potential to act as tools for detection of xylulose. Using a two-plasmid reporter system in Escherichia coli , we investigated the regulatory protein component (MtlR) of the mannitol operon from Pseudomonas fluorescens . Our findings suggest that the promoter of mtlE is activated by MtlR in the presence of a variety of sugar inducer molecules, and may exhibit cross-activity with a native regulator of E. coli . Additionally, we engineered the L-arabinose transcriptional activator (AraC) of E. coli for altered ligand specificity. We performed site-specific saturation mutagenesis to generate AraC variants with altered effector specificity, with an aim to generate a mutant activated by xylulose. We characterized several mutant AraC variants which have lost the ability to respond specifically to the native L-arabinose effector. We promote this technique as a powerful tool for future iGEM teams to create regulatory circuits activated by novel small molecule ligands.
The <i>Azotobacter vinelandii</i> AlgE mannuronan C‐5‐epimerase family is essential for the <i>in vivo</i> control of alginate monomer composition and for functional cyst formation (2008)

The industrially widely used polysaccharide alginate is a co-polymer of beta-D-mannuronic acid and alpha-L-guluronic acid (G), and the G residues originate from a polymer-level epimerization process catalysed by mannuronan C-5-epimerases. In the genome of the alginate-producing bacterium Azotobacter vinelandii genes encoding one periplasmic (AlgG) and seven secreted such epimerases (AlgE1-7) have been identified. Here we report the generation of a strain (MS163171) in which all the algE genes were inactivated by deletion (algE1-4 and algE6-7) or interruption (algE5). Shake flask-grown MS163171 produced a polymer containing less than 2% G (algG still active), while wild-type alginates contained 25% G. Interestingly, addition of proteases to the MS163171 growth medium resulted in a strong increase in the chain lengths of the alginates produced. MS163171 was found to be unable to form functional cysts, which is a desiccation-resistant differentiated form developed by A. vinelandii under certain environmental conditions. We also generated mutants carrying interruptions in each separate algE gene, and a strain containing algE5 only. Studies of these mutants indicated that single algE gene inactivations, with the exception of algE3, did not affect the fractional G content much. However, for all strains tested the alginate composition varied somewhat as a response to the growth conditions.
Determination of the fluorescence quantum yields of some 2-substituted benzothiazoles (1984)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDetermination of the fluorescence quantum yields of some 2-substituted benzothiazolesG. F. Kirkbright, D. E. M. Spillane, Kevin. Anthony, R. G. Brown, J. D. Hepworth, K. W. Hodgson, and M. A. WestCite this: Anal. Chem. 1984, 56, 9, 1644–1647Publication Date (Print):August 1, 1984Publication History Published online1 May 2002Published inissue 1 August 1984https://pubs.acs.org/doi/10.1021/ac00273a024https://doi.org/10.1021/ac00273a024research-articleACS PublicationsRequest reuse permissionsArticle Views435Altmetric-Citations12LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Targeted deletion of the Nesp55 DMR defines another <i>Gnas</i> imprinting control region and provides a mouse model of autosomal dominant PHP-Ib (2010)

Approximately 100 genes undergo genomic imprinting. Mutations in fewer than 10 imprinted genetic loci, including GNAS, are associated with complex human diseases that differ phenotypically based on the parent transmitting the mutation. Besides the ubiquitously expressed Gsalpha, which is of broad biological importance, GNAS gives rise to an antisense transcript and to several Gsalpha variants that are transcribed from the nonmethylated parental allele. We previously identified two almost identical GNAS microdeletions extending from exon NESP55 to antisense (AS) exon 3 (delNESP55/delAS3-4). When inherited maternally, both deletions are associated with erasure of all maternal GNAS methylation imprints and autosomal-dominant pseudohypoparathyroidism type Ib, a disorder characterized by parathyroid hormone-resistant hypocalcemia and hyperphosphatemia. As for other imprinting disorders, the mechanisms resulting in abnormal GNAS methylation are largely unknown, in part because of a paucity of suitable animal models. We now showed in mice that deletion of the region equivalent to delNESP55/delAS3-4 on the paternal allele (DeltaNesp55(p)) leads to healthy animals without Gnas methylation changes. In contrast, mice carrying the deletion on the maternal allele (DeltaNesp55(m)) showed loss of all maternal Gnas methylation imprints, leading in kidney to increased 1A transcription and decreased Gsalpha mRNA levels, and to associated hypocalcemia, hyperphosphatemia, and secondary hyperparathyroidism. Besides representing a murine autosomal-dominant pseudohypoparathyroidism type Ib model and one of only few animal models for imprinted human disorders, our findings suggest that the Nesp55 differentially methylated region is an additional principal imprinting control region, which directs Gnas methylation and thereby affects expression of all maternal Gnas-derived transcripts.
Solvatofluorochromism and twisted intramolecular charge‐transfer state of the nile red dye (2012)

Abstract Profiles of the S 1 potential energy surface of the Nile Red dye along the rotational coordinate of the amino group are computed using time‐dependent density functional theory (TDDFT) and XMCQDPT2/CASSCF. The calculated profiles exhibit two minima corresponding to a planar locally excited (LE) state and a twisted intramolecular charge transfer (TICT) state. The profiles calculated by time‐dependent density functional theory (TDDFT) depend on the weight of the hartree‐fock (HF) exchange in the functional: at 0% exchange, only the TICT minimum exists, whereas at 50% exchange and more there is only the LE minimum. The profiles obtained by TDDFT at 20–25% HF exchange are in qualitative agreement with that obtained by XMCQDPT2/CASSCF calculations. The energy of the charge transfer state is lowered due to the participation of doubly excited configurations and dynamic correlations, which is implicitly included in the density functionals. The solvent effects on the relative energies of the LE and TICT states and on the barrier height are studied using the polarizable continuum model. The effect of hydrogen bonds is studied for a complex of Nile Red with two water molecules. The solvatochromism of Nile Red fluorescence in aprotic polar solvents is explained by nonspecific solvation, which stabilizes the LE state and causes Nile Red fluorescence solvatochromism; in water and alcohols, it is explained by the formation of hydrogen bonds, which stabilize the TICT state and facilitate the LE‐to‐TICT transition. © 2012 Wiley Periodicals, Inc.
Dysfunctional epileptic neuronal circuits and dysmorphic dendritic spines are mitigated by platelet-activating factor receptor antagonism (2016)

Temporal lobe epilepsy or limbic epilepsy lacks effective therapies due to a void in understanding the cellular and molecular mechanisms that set in motion aberrant neuronal network formations during the course of limbic epileptogenesis (LE). Here we show in in vivo rodent models of LE that the phospholipid mediator platelet-activating factor (PAF) increases in LE and that PAF receptor (PAF-r) ablation mitigates its progression. Synthetic PAF-r antagonists, when administered intraperitoneally in LE, re-establish hippocampal dendritic spine density and prevent formation of dysmorphic dendritic spines. Concomitantly, hippocampal interictal spikes, aberrant oscillations, and neuronal hyper-excitability, evaluated 15-16 weeks after LE using multi-array silicon probe electrodes implanted in the dorsal hippocampus, are reduced in PAF-r antagonist-treated mice. We suggest that over-activation of PAF-r signaling induces aberrant neuronal plasticity in LE and leads to chronic dysfunctional neuronal circuitry that mediates epilepsy.
Free Cupric Ion Activity in Seawater: Effects on Metallothionein and Growth in Crab Larvae (1983)

Crab zoeae (Rhithropanopeus harrisii) were exposed during their development opment to a range of free cupric ion activities regulated in seawater by use of a copper chelate buffer system. Most cytosolic copper was found to be associated with metallothionein. Copper-thionein could be related to free cupric ion activity, and a shift in copper-thionein accumulation was correlated with inhibition of larval growth. These data reveal predictable relations between cupric ion activity in seawater and processes at the cellular and organismic levels.
THE BEVATRON MAGNET PULSE-TIMING SYSTEM (1955)

The University of Calif())rnia Bevatron magnet-pulsing system provides a means of adjusting the pulse -repetition rate and the pulse length of current flowing in the magnet.The unit also provides synchroni~ing pulses for the.linear accelerator injector as well as various trigger pulses before, during, and after the flow of magnet current.The unit derives its timing pulses rom a master oscillator operating at 2 ppe.Pulses from the master oscillator are scaled down to produce the selectable repetition rates for the magnet current.All timing functions are derived fr.om standard time..:d.elay circuits except for the magnet pulse length which is generated by a, new type of Schmitt trigger .circuit... .
END STAGE POLYCYSTIC KIDNEY DISEASE: INDICATIONS AND TIMING OF NATIVE NEPHRECTOMY RELATIVE TO KIDNEY TRANSPLANTATION (2004)

A49 Aims: We evaluated indications and outcome of pre-transplant, concomitant and post-transplant native nephrectomy in patients with end stage polycystic kidney disease (PCKD). Methods: The records of 32 patients were retrospectively reviewed using our institution’s electronic database. Results: Between Jan. 1992 and Dec. 2002, 171 patients with end stage PCKD received a kidney transplant at our institution. Among those, 32 patients underwent either pre-transplant (A) concomitant (B) or post-transplant (C) native nephrectomy. Bilateral nephrectomy was performed in 25 patients. Mean follow-up was 23.3 months. In group A (n = 7) indications for nephrectomy were hematuria, a renal mass and chronic pain. In B (n = 16) lack of space was the main indication. In C (n = 9) the main indication was urinary tract infection. Operating time was 231±14 min (A) vs. 370±24 min (B) vs. 208±14 min (C) [P = 0.001]. Intraoperative blood loss was 533±105 ml (A) vs. 573±155 ml (B) vs. 522±181 ml (C) [P = n.s.]. In group B two patients required blood transfusions. Perioperative complications included a wound dehiscence (A) and abdominal bleeding (C). Hospital stay was comparable between groups: 7±0.7 days (A), 8.6±1.2 days (B) and 6.3±0.6 days (C) [P = n.s.]. At 3 months, serum-creatinine was not significantly different between group B and C: 1.3±0.1mg/dl vs. 1.5±0.2 mg/dl. Conclusions: Unilateral or bilateral nephrectomy of polycystic kidneys at the time of transplantation has a low complication rate and does not increase patient morbidity. Postoperative graft function is excellent. We therefore perform concomitant native nephrectomy whenever indicated. INDICATIONS FOR NATIVE NEPHRECTOMY IN PCKD PATIENTS Pre-transplant: hematuria requiring transfusions / renal mass >> bilateral nephrectomy Concomitant: lack of space for kidney graft / chronic pain >> unilateral or bilateral nephrectomy chronic infection / hematuria >> bilateral nephrectomy Post-transplant: de novo infection / renal mass / hematuria / chronic pain >> bilateral nephrectomy
Clonogenic Patterns of Human Pulmonary Adenocarcinoma Cell Lines (PC-9, PC-13 and PC-14) and How They Influence the Results of Test for Chemosensitivity to Cisplatin in the Human Tumor Clonogenic Assay (1985)

The clonogenic patterns of three human pulmonary adenocarcinoma cell lines (PC-9, PC-13 and PC-14) were studied by human tumor clonogenic assay (HTCA), and factors which could influence the results of tests for the chemosensitivity of these tumor cells to cisplatin in HTCA were determined. The results showed that each tumor cell line had a characteristic clonogenic pattern. The time intervals for the cells to grow to the plateau phase varied from 9 to 16 days, depending on the cell line and number of cells plated. The number of cells plated could substantially influence the results of chemosensitivity tests. The percentage of surviving colonies increased markedly if too many cells (usually 5 X 10(4) or more/plate) were plated. For continuous exposure, the results of chemosensitivity tests were rather stable after 7 days of incubation in each cell line, especially when fewer than 2 X 10(4) cells/plate were plated. For 1-hour exposure, the incubation periods for the results to become stable varied from 7 to 16 days depending on the cell type, number of cells plated and drug concentration. It was stressed that for the correct evaluation of the chemosensitivity of cultured cell lines in HTCA, the clonogenic pattern of each tumor cell line should be checked in detail before further experiments are conducted. The higher the concentration and the longer the exposure time, the more strongly cis-diamminedichloroplatinum (CDDP) suppressed the colony growth in each of the three cell lines in HTCA, and it was recommended that CDDP should be used clinically in such a way as to maintain a high serum level of the active form of CDDP for a long time.
Evolution of Software Composition Mechanisms: A Survey (2007)

This chapter contains sections titled: Introduction Basic Concepts Early Days Achieving Flexibility Software Composition in the Open World Challenges and Future Work Acknowledgments References ]]>