gebhart/patrepeval huggingface dataset:
Relationship of Stereochemical and Skeletal Diversity of Small Molecules to Cellular Measurement Space (2004)

Systematic and quantitative measurements of the roles of stereochemistry and skeleton-dependent conformational restriction were made using multidimensional screening. We first used diversity-oriented synthesis to synthesize the same number (122) of [10.4.0] bicyclic products (B) and their corresponding monocyclic precursors (M). We measured the ability of these compounds to modulate a broad swath of biology using 40 parallel cell-based assays. We analyzed the results using statistical methods that revealed illuminating relationships between stereochemistry, ring number, and assay outcomes. Conformational restriction by ring-closing metathesis increased the specificity of responses among active compounds and was the dominant factor in global activity patterns. Hierarchical clustering also revealed that stereochemistry was a second dominant factor; whereas the stereochemistry of macrocyclic appendages was a determinant for bicyclic compounds, the stereochemistry of the carbohydrates was a determinant for the monocyclic compounds of global activity patterns. These studies illustrate a quantitative method for measuring stereochemical and skeletal diversity of small molecules and their cellular consequences.
Synthesis and characterization of histidine‐phosphorylated peptides (1997)

Posttranslational phosphorylation of proteins is an important event in many cellular processes. Whereas phosphoesters of serine, threonine, and tyrosine have been studied extensively, only limited information is available for other amino acids modified by a phosphate group. The formation of phosphohistidine residues in proteins was discovered originally in prokaryotic organisms, but also has been found recently in eukaryotic cells. We describe methods for the synthesis and analysis of phosphohistidine-containing peptides, a prerequisite for the investigation of the role of this posttranslational modification in cellular processes.
Simulation aided dosing control of a 2D array therapeutic ultrasound transducer (2010)

Setting proper input power and duty cycle to produce a desired thermal dose is a non-trivial task in ultrasound thermal therapy. This paper presents an approach to estimate these parameters based on computer simulations. An in-house custom 2D therapeutic phased array was used for the studies. Acoustic power measurements were used to provide input values and validation for the pressure and temperature field simulations. Simulated pressure, intensity and temperature fields were in turn used to optimize the dosing control parameters. Simulation-projected dosing parameters were verified in a gellum-gum based tissue mimicking phantom (TMM) with 75μm butt-welded thermocouples (TC) embedded inside. The time averaged input powers were varied from 12W to 25W. A 30s dose was applied at each setting, and the temperatures at the focus and a location 2mm away from the focus in array azimuth direction were acquired. Results showed that the peak end-of-dose temperature rise was approximately linearly proportional to the input acoustic power. Simulations predicted a similar linear relationship with less than 10% error from the experimental results. Ex-vivo bovine liver studies also showed good correlation between the simulation-predicted lesion and the experimental lesion; similar patterns were observed. The results demonstrated that by combining simulations with force balance measurement, dosing instructions for any desired focal temperature can be quickly and easily produced.
Sensitivity and specificity of spinal inflammatory lesions assessed by whole‐body magnetic resonance imaging in patients with ankylosing spondylitis or recent‐onset inflammatory back pain (2009)

Diagnostic utility of STIR MRI for AS is optimal when > or =2 CIL are present. A single CIL can be found in up to 26% of healthy individuals.
Cyclooxygenase-2 inhibitor NS398 preserves neuronal function after hypoxia/ischemia in piglets (2001)

Anoxic stress attenuates NMDA-induced pial arteriolar dilation via a mechanism involving actions of cyclooxygenase (COX)-derived reactive oxygen species (ROS). We examined whether the selective COX-2 inhibitor NS398 would protect neuronal function after global hypoxia/ischemia (H/I) in piglets. Pial arteriolar responses to NMDA (10-100 micromol/l) were determined using intravital microscopy in anesthetized piglets before and 1 h after H/I. Study groups received vehicle, 0.3, 1, or 5 mg/kg NS398, or 0.3 mg/kg indomethacin (n = 7, 6, 6, 5 and 8, respectively) i.v. 20 min prior to H/I. H/I reduced NMDA- induced dilation to 44 +/- 6% (100 micromol/l NMDA, mean +/- s.e.m.) of the pre-ischemic response in vehicle animals (p < 0.05). However, NS398 dose-dependently protected arteriolar dilation to NMDA (77 +/- 8, 81 +/- 16, and 102 +/- 10% preservation at 0.3, 1 and 5 mg/kg, respectively). Indomethacin caused similar preservation. However, indomethacin but not NS398 reduced serum thromboxane B(2) levels to undetectable values. In conclusion, COX-2 appears to be a major source of ROS in the piglet cerebral cortex after H/I.
TSC simulation of Ohmic discharges in TFTR (1993)

The Tokamak Simulation Code (TSC) has been used to model the time dependence of several Ohmic discharges in the TFTR experiment. The semi-empirical thermal conductivity model and the sawtooth model in TSC have been refined so that good agreement between the simulation and the experiment is obtained in the electron and ion temperature profiles and in the current profiles for the entire duration of the discharges. Neoclassical resistivity gives good agreement with the measured surface voltage and the rate of poloidal flux consumption
Hemodynamic responses to <i>Pasteurella haemolytica</i> inoculation in calves given type 2 serotonergic antagonist (1996)

peer reviewed
Wirksamkeit von Etidronat und sequenziellem Monofluorphosphat bei schwerer postmenopausaler Osteoporose (2006)

Zusammenfassung Innerhalb einer dreijährigen Pilotstudie an 52 Frauen mit schwerer postmenopausaler Osteoporose wurden die Behandlungen mit Etidronat und anschließender Gabe von Kalzium und Vitamin D (ECaD) einerseits bzw. mit Etidronat und anschließender Verabreichung von Monofluorphosphat plus Kalzium und Vitamin D (EFCaD) andererseits miteinander verglichen. Die Knochenmineraldichte (BMD) von Lendenwirbelsäule, Gesamthüfte und Schenkelhals nahm mit dem EFCaD-Regime signifikant stärker zu als mit dem ECaD-Regime. Der Score für Schmerzen/Mobilität ging mit EFCaD signifikant stärker zurück als mit ECaD (p=0,006).Neue vertebrale Frakturen traten beidrei Patientinnen unter EFCaD (12%) bzw. neun Patientinnen unter ECaD (35%) auf (p = 0,048). Bei drei Patientinnen unter EFCaD (12%) bzw. 15 Patientinnen unter ECaD (58%) war kein Ansprechen (Response) auf die Therapie zu verzeichnen (p-Wert des Unterschieds = 0,001). Bei 25 Patientinnen wurde über leichte bis mittelschwere unerwünschte Ereignisse berichtet, wobei zwischen den beiden Behandlungsgruppen keine signifikanten Unterschiede zu beobachten waren. Die Pilotstudie deutet darauf hin, dass Etidronat, sequenziell gefolgt von Monofluorphosphat, eine sichere, wirksame und relativ kostengünstige Therapie bei schwerer postmenopausaler Osteoporose darstellen könnte.
[Sexual transmissibility of papillomaviruses]. (1985)

Papillomavirus infections in the genitoanal region are detectable by the demonstration of type-specific HPVDNA (DNA-DNA hybridization, in situ hybridization on cell smears) or by a peroxidase-antiperoxidase assay using formalin-fixed paraffin sections for demonstration of common structural antigens of papillomaviruses. Using these methods an epidemiological study on sexual partners with genitoanal papillomavirus infections has been initiated.
Direct analysis of slags by inductively coupled plasma atomic emission spectrometry using slurry sample introduction techniques (1991)

A practical method is described for the direct determination of Si, Ca, Mg, Al, Fe, Mn, Ti, Na and K in slag samples by the introduction of sample suspensions into the inductively coupled plasma atomic emission spectrometer. The effect of the particle size distribution on the atomization efficiency of these elements when slurries of the slag samples were aspirated into the plasma has been studied. The slurry samples were prepared by dispersing 0.1 g of ground slag in 100 ml of 0.35% ammonia solution; calibration was achieved by the use of a reference slag prepared in the same manner. The results obtained for the direct analysis of slag slurries using such a procedure were in good agreement with those obtained by classical dissolution of the sample. The reproducibility of the results with slurry nebulization (concentration range 1.7–5.5%) were poorer than those observed with aqueous solutions (concentration range 0.5–4.8%) but were acceptable for slag industrial control.
Biological parameters of Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) assayed with Bacillus thuringiensis berliner (2005)

The bacterium Bacillus thuringiensis (Bt) is a promising control agent of Spodoptera frugiperda (J.E. Smith). Besides the lethal effect on hosts, the entomopathogen may cause physiological alterations that hinder insects' feeding intake and reproduction. Larval and female pupa weight, oviposition and egg fecundity were determined in second instar S. frugiperda larvae infected with 39 Bt isolates in four bioassays. Sublethal effects were observed in the follow isolates: ESALQ 1.1, ESALQ 1.4, ESALQ 1.6, ESALQ 3.7, ESALQ 4.2 and ESALQ 5.2 (larval and female pupae weight); ESALQ 5.1 (larval weight); ESALQ 1.2 and ESALQ 3.7 (egg fecundity); ESALQ 3.7 (oviposition and egg fecundity); ESALQ 3.7 and ESALQ 5.2 (oviposition). Significant correlations were observed when parameters were analyzed together, except in the second bioassay (female pupal weight ´ oviposition, female pupal weight ´ egg fecundity and oviposition ´ egg fecundity). Some Bt isolates affected the biological parameters of S. frugiperda, mainly larval and female pupae weight, and in some instances these effects were passed on to further life cycle stages affecting oviposition and egg fecundity. Although sublethal effects are difficult to evaluate, mainly under field conditions, they should be considered when the activity of an entomopathogen is measured.
Preparation and partial characterization of sheep erythrocyte-antibody-complement intermediate. (1968)

A reagent has been prepared which contains C′(4), C′(2), C′(3), C′(5), C′(6) and C′(7) by chromatography of guinea-pig serum on CM-cellulose. With this reagent, when supplemented with additional C′(5), EAC′(1a,4,2a,3,5,6,7) was prepared from EAC′(1a). The reactivity and stability of cells possessing active C′(7) sites prepared with this reagent appeared to be the same as EAC′(1a,4,2a,3,5,6,7) prepared from EAC′(1a,4) and mixtures of individual functionally pure components. The reactivity of cells possessing active C′(7) sites has been measured by initial velocity of generation of C′(8) sites, and an inhibitor of this reaction, Antrypol, has been described. A method for the estimation of the average number of C′(7) sites was investigated and a correlation appears to exist between the average number of sites per cell and the initial velocity of reaction with C′(8). Finally, a provisional method for the assay of C′(8) and C′(9) has been described.
Integrin α5/β1 Mediates Fibronectin-dependent Epithelial Cell Proliferation through Epidermal Growth Factor Receptor Activation (2000)

Human integrin alpha5 was transfected into the integrin alpha5/beta1-negative intestinal epithelial cell line Caco-2 to study EGF receptor (EGFR) and integrin alpha5/beta1 signaling interactions involved in epithelial cell proliferation. On uncoated or fibronectin-coated plastic, the integrin alpha5 and control (vector only) transfectants grew at similar rates. In the presence of the EGFR antagonistic mAb 225, the integrin alpha5 transfectants and controls were significantly growth inhibited on plastic. However, when cultured on fibronectin, the integrin alpha5 transfectants were not growth inhibited by mAb 225. The reversal of mAb 225-mediated growth inhibition on fibronectin for the integrin alpha5 transfectants correlated with activation of the EGFR, activation of MAPK, and expression of proliferating cell nuclear antigen. EGFR kinase activity was necessary for both MAPK activation and integrin alpha5/beta1-mediated cell proliferation. Although EGFR activation occurred when either the integrin alpha5-transfected or control cells were cultured on fibronectin, coprecipitation of the EGFR with SHC could be demonstrated only in the integrin alpha5-transfected cells. These results suggest that integrin alpha5/beta1 mediates fibronectin-induced epithelial cell proliferation through activation of the EGFR.
Opportunities for Service-Learning Partnerships in the Information Systems Curriculum (2011)

Service-learning partnerships involve students taking part in community service that relates to their academic course experience. Students who major in information systems are increasingly being provided with real-world experiences. These real-world experiences offer numerous benefits. Students can immediately apply their course knowledge to real-world situations. Students can get the experience of grappling with political, social, and ethical issues in a workplace setting. In addition, students can develop a sense of civic responsibility, by contributing their skills to their communities. This chapter presents the issues involved in implementing the service-learning paradigm in an information systems curriculum. Examples of successful service-learning courses are presented, and new possibilities for service-learning courses are discussed.Request access from your librarian to read this chapter's full text.
Mechanical–Dynamic Test Methods for Lubricants (2006)

This chapter contains sections titled: Tribological System Categories within Lubricant Tests Simple Mechanical-Dynamic Lubricant Test Machines Four-ball Apparatus Reichert's Friction-wear Balance, Brugger Apparatus Falex Test Machines Falex Block-on-ring Test Machine The Falex Pin and Vee Block Test Machine Falex High-performance Multispecimen Test Machine Falex Tapping Torque Test Machine Timken Test Machine High-frequency Reciprocating Test Machines High-frequency Reciprocating Rig (HFRR) High-frequency, Linear-oscillation Test Machine (SRV) Mini Traction Machine (MTM) Low-velocity Friction Apparatus (LVFA), Tribometer Diesel Injector Apparatus Performance Tests for Gear Oil Applications FZG Gear-test Rig FZG EP Tests – Scuffing FZG High-EP Tests – Scuffing Load Capacity FZG Pitting Tests FZG Micro-Pitting Tests FZG Wear Tests FZG Gear-efficiency Tests FZG Synthetic Oil Ageing Tests Performance Tests for Roller Bearing Applications FAG Roller Bearing Test Apparatus FE8 FAG Roller Bearing Test Apparatus FE9 Performance Tests for Synchronizer Applications Area of Application Function of the Synchronizer Standardized Test Rigs and Test Methods μ-Comb Synchronizer Testing Machine FZG SSP180 Synchronizer Testing Machine Performance Tests for Automatic Transmissions Area of Application Function of Friction Disks and Fluid Standardized Test Rigs and Test Methods SAE 2 Machine DKA Machine ZF GK Test Bench 2 Performance Tests for Continuously Variable Transmissions Field of Application Function of Chain, Push-Belt and Fluid Standardized Test Rigs and Test Methods ZF Universal Variator Test Bench Van Doorne Test Bench Performance Tests for Hydraulic Fluid Applications Field of Application Function of Vane Pumps Standardized Vane Pump Test Machines and Test Methods Function of Axial Piston Pumps Standardized Axial Piston Pump Test Machines and Test Methods Nonstandardized Specialized Testing, Efficiency Tests Hybrid Pump Testing Other Standardized and Non-Standardized Test Methods and Test Machines for Lubricants Interpretation and Precision of Lubricant Tests
PROGRESS IN CHERENKOV RING IMAGING Part 2: Identification of charged hadrons at 200 <font>GeV</font>/c (1995)

Abstract We have used a ring-imaging Cherenkov detector to separate π's, K's, and antiprotons in a 200 GeV/ c beam at Fermilab. This device was built as a prototype for a large-aperture counter now in operation in Fermilab experiment E605. The radiator consisted of 8 m of atmospheric-pressure helium gas. The photon detector was a multistep proportional chamber. Cherenkov photons near 8 eV were detected by photoionization of triethylamine (TEA) vapor in the chamber. An average of 2.5 to 2.7 Cherenkov photons were observed per event, corresponding to a figure of merit N 0 ⋍ 45 per cm. A single-photon radius uncertainty of 0.47 mm was obtained with a helium/TEA/CH 4 gas mixture in the photon detector. The rms uncertainty in the determination of the Cherenkov angle was ΔΘ c /Θ max = 0.006 , corresponding to one-standard-deviation π/K separation at 500 GeV/ c . At 200 GeV/ c , the particle identification efficiency in a beam containing 95.2% π − , 4.3% K − , and 0.5% antiprotons was 92% for the π's, 83% for the K's, and 90% for the antiprotons.
Modularity and the Independence of Functional Requirements in Designing Complex Systems (1994)

Abstract We are investigating principles for the design of product structures that can be readily adapted in response to large changes in customer requirements. In this paper, we report on a part of our investigation, namely, the effect of Suh’s independence axiom on achieving a modular design for a hierarchical system. We demonstrate that there is a relationship between achieving functional independence and reducing the interactions between modules by comparing the functional independence of several designs reflecting different requirements on module interfaces for a portal frame. Open research questions are identified. Our desire in writing this paper is to describe some work in progress and foster discussion rather than offering a prescription for solution.
Dementia with ALS features and diffuse Pick body-like inclusions (atypical Pick's disease?). (1995)

An autopsy case of dementia beginning with right hand muscle atrophy was reported. A 42-year-old woman with no family history of neurologic disease developed weakness of the right hand at age 30, and was diagnosed as having amyotrophic lateral sclerosis (ALS). The weakness and atrophy spread to the four extremities subsequently. At age 36, she could not walk without assistance. At age 38, she was first noted as having dementia with forced crying, and she also showed generalized muscle weakness and atrophy. She rapidly developed akinetic mutism, and died of respiratory failure at age 42, 12 years after the onset of the symptoms. Macroscopically, the brain showed fronto-temporal atrophy. Microscopic examination revealed numerous intracytoplasmic inclusions throughout the central nervous system (CNS) including anterior horn of the spinal cord. The inclusions were stained moderate to dark brown with silver stain. Electron microscopically, they consisted of fibrils covered along most of the length with granular and fuzzy materials. This case was thought to be difficult to categorize in any known neuro-degenerative diseases. We proposed the case to be "atypical Pick's disease" with ALS features. This case might be a new entity of neuro-degenerative disease.
Bone sialoprotein—a mucin in disguise? (1996)

Connective Tissue Biology Group, The Department of BiomedicalEngineering, The Research Institute of The Cleveland Clinic Foundation,Cleveland, OH 44195, USA'To whom correspondence should be addressed at: Department ofBiomedical Engineering/Wb3, Research Institute of the Cleveland ClinicFoundation, 9500 Euclid Avenue, Cleveland, OH 44195
Identification of a peroxisome proliferator-responsive element upstream of the gene encoding rat peroxisomal enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase. (1992)

Ciprofibrate, a hypolipidemic drug that acts as a peroxisome proliferator, induces the transcription of genes encoding peroxisomal beta-oxidation enzymes. To identify cis-acting promoter elements involved in this induction, 5.8 kilobase pairs of promoter sequence from the gene encoding rat peroxisomal enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase (EC 4.2.1.17/EC 1.1.1.35) was inserted upstream of a luciferase reporter gene. Transfection of this expression vector into rat hepatoma H4IIEC3 cells in the presence of ciprofibrate resulted in a 5- to 10-fold, cell type-specific increase in luciferase activity as compared to cells transfected in the absence of drug. A peroxisome proliferator-responsive element (PPRE) was localized to a 196-nucleotide region centered at position -2943 from the transcription start site. This PPRE conferred ciprofibrate responsiveness on a heterologous promoter and functioned independently of orientation or position. Gel retardation analysis with nuclear extracts demonstrated that ciprofibrate-treated or untreated H4IIEC3 cells, but not HeLa cells or monkey kidney cells, contained sequence-specific DNA binding factors that interact with the PPRE. These results have implications for understanding the mechanisms of coordinated transcriptional induction of genes encoding peroxisomal proteins by hypolipidemic agents and other peroxisome proliferators.
Experimental results for matched filter construction and correlation with a single curved element (1994)

We demonstrate in the laboratory the construction and correlation of a matched filter correlator using only a single curved element. Previously we demonstrated a correlator that uses a converging reference beam for constructing a matched filter so that when performing correlation, the correlator does not require a lens for taking the inverse Fourier transform. This paper demonstrates an extension in which we use a diverging beam from a laser diode for constructing the hologram in order to avoid a lens for generating a converging beam. The matched filter is turned upside down and reversed for use in the correlator.
Comparative measurements of radiation-induced DNA double-strand breaks by graded voltage and pulsed-field gel electrophoresis (1997)

We compared the ability of graded-voltage gel electrophoresis (GVGE) and pulsed-field gel electrophoresis (PFGE), to resolve DNA containing double-strand breaks (dsb) induced in human MS751 cells after exposure to gamma-radiation. For quantitation, prelabelling of the cells with [2-14C]-thymidine prior to electrophoresis and subsequent scintillation counting of excised bands was found to give closely similar results to Southern blotting and radiolabelled probe hybridization followed by phosphorimager quantitation. Compared with PFGE, DNA subjected to GVGE migrated further and generated distinct DNA bands. Dsb were detected and quantifiable with GVGE at much lower doses than with PFGE the radiation dose limits were approximately 2 and 10 Gy respectively. At all doses used, the amounts of dsb detected by GVGF, were higher than those by PFGE. GVGE coupled with Southern hybridization and phosphorimager analysis is thus a more sensitive approach to assessing dsb and their relationship with radiation sensitivity. The approach is also convenient when processing large numbers of samples and has the additional advantage of avoiding cell prelabelling such as in the case of cells extracted directly from human tumour biopsies and normal tissues.
Mn-Salen Catalyzed Asymmetric Epoxidation of Conjugated Trisubstituted Olefins (1995)

Epoxidation of trisubstituted olefins using Mn-salen complex (1) as a catalyst proceeds with high enantioselectivity and the sense of asymmetric induction observed in this reaction could be rationalized mainly by electronic repulsion between salen-benzene ring and unsaturated olefinic substituents.