gebhart/patrepeval huggingface dataset:
Npa1p is an essential <i>trans</i>-acting factor required for an early step in the assembly of 60S ribosomal subunits in <i>Saccharomyces cerevisiae</i> (2004)

Ribosome biogenesis requires >100 nonribosomal proteins, which are associated with different preribosomal particles. The substrates, the interacting partners, and the timing of action of most of these proteins are largely unknown. To elucidate the functional environment of the putative ATP-dependent RNA helicase Dbp6p from Saccharomyces cerevisiae, which is required for 60S ribosomal subunit assembly, we have previously performed a synthetic lethal screen and thereby revealed a genetic interaction network between Dbp6p, Rpl3p, Nop8p, and the novel Rsa3p. In this report, we extended the characterization of this functional network by performing a synthetic lethal screen with the rsa3 null allele. This screen identified the so far uncharacterized Npa1p (YKL014C). Polysome profile analysis indicates that there is a deficit of 60S ribosomal subunits and an accumulation of halfmer polysomes in the slowly growing npa1-1 mutant. Northern blotting and primer extension analysis shows that the npa1-1 mutation negatively affects processing of all 27S pre-rRNAs and the normal accumulation of both mature 25S and 5.8S rRNAs. In addition, 27SA(2) pre-rRNA is prematurely cleaved at site C(2). Moreover, GFP-tagged Npa1p localizes predominantly to the nucleolus and sediments with large complexes in sucrose gradients, which most likely correspond to pre-60S ribosomal particles. We conclude that Npa1p is required for ribosome biogenesis and operates in the same functional environment of Rsa3p and Dbp6p during early maturation of 60S ribosomal subunits.
Omnidirectional optical mirror in a cladded-superlattice structure (2002)

In recent years, it has been shown that one-dimensional photonic crystals such as superlattices can exhibit an omnidirectional reflection of light for a given frequency range. However, this property requires that the incident wave be launched from vacuum or from a low refractive index material. In this article, we show that the limitation about the choice of the substrate can be removed by adding a clad layer of low refractive index at one boundary of the superlattice. The additional layer acts like a barrier for the propagation of light. An alternative solution based on a combination of two multilayer structures is also mentioned.
Using Interactive Graphics for Fitting Surfaces to Scattered Data (1984)

Robust software for reconstructing a surface from scattered data has been implemented in an interactive system with graphical facilities and with highly modular machine-independent structure. Particular attention is paid to the mathematical capabilities of the system, which consist of a wide range of numerical methods for constructing a smooth bivariate function F( <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">x</i> , <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">y</i> ) that approximates or interpolates certain prescribed values <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">f</i> <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">k</sub> at the points ( <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">x</i> <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">k</sub> , <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">y</i> <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">k</sub> ), 1, . . ., N. These points must be distinct and noncolinear. Since this is a typical laboratory computer problem, the system has been designed for a minicomputer, and the need for both machine and operating system independence has been taken into account.
Treatment of fetal cardiac arrhythmias. (1985)

Fetal cardiac arrhythmias are usually first suspected on the basis of auscultatory findings during routine examination in pregnancy. With improved ultrasonic equipment a more accurate diagnosis of the type of arrhythmia can be made, associated problems such as structural cardiac defects and cardiac compromise can be recognized, and the effects of intrauterine treatment schedules can be closely monitored. The success of intrauterine therapy depends first and foremost on accurate intrauterine diagnosis. Until now intrauterine therapy for fetal cardiac arrhythmias has remained almost entirely restricted to pharmacological interventions.
Synthesis, Crystal Growth and Characterization of <scp>l</scp>-Proline Lithium Chloride Monohydrate: A New Semiorganic Nonlinear Optical Material (2009)

A new semiorganic material, l-proline lithium chloride monohydrate (LPLCM), was synthesized for the first time. Its solubility and metastable zonewidth in double distilled water were estimated. Employing a temperature reduction method, a crystal of size 16 × 6 × 5 mm3 was grown from aqueous solution. The cell dimensions obtained by single crystal X-ray diffraction studies reveal that the crystal belongs to the monoclinic system. Functional groups of the grown crystal were identified from FTIR spectral analysis. UV−vis−NIR studies show that the crystal is transparent in the wavelength range of 300−1100 nm. Second harmonic generation conversion efficiency found using the Kurtz and Perry method is about 0.2 times that of KDP. The thermal stability of the compound was determined by TG-DTA analyses of the specimen. The microhardness test was carried out, and the load dependent hardness was measured.
CONCORRÊNCIA COMPUTACIONAL: na disciplina de Fundamentos de Sistemas Operacionais (2019)

Quando falamos em sistemas operacionais multiprogramaveis um principio basico e o mecanismo de concorrencia, mas o que e concorrencia? Segundo Russo (2012), um processador pode executar instrucoes em paralelo operando por entradas e saidas onde e permitido que varias atividades sejam executadas ao mesmo tempo, ou seja fazendo as CPU, concorrerem com a mesma tarefa e um programa perde o uso do processador, mas depois retoma e continua a execucao da atividade da mesma maneira que no momento no qual foi finalizado,  entao resumindo concorrencia e quando processador tem a possibilidade de executar duas mais tarefas simultaneamente permitindo que varios programas sejam executados concorrentemente pelo sistema operacional.
An improved method for determining high molecular weights by protein immunoblotting: application to apolipoprotein(a) phenotyping. (1991)

The determination of very high molecular weights (greater than 500 kd) by protein immunoblotting is limited by a lack of commercially available molecular weight standards in that range. We have therefore investigated the use of the human haptoglobin 2-2 phenotype polymeric series as a high molecular weight standard curve. Using pre-electrophoresed 3-12% SDS polyacrylamide gradient gels, non-dissociated haptoglobin 2-2 serum was separated, transferred and probed with anti-human haptoglobin. We were able to visualize up to 13 bands, with a theoretical molecular weight range of 171.9-859.5 kd, in non-dissociated haptoglobin 2-2 serum. Molecular weights up to 584 kd were confirmed by comparison with available standards. The method was then applied to the determination of apolipoprotein(a) [apo(a)] phenotype molecular weights. Precision of the method was excellent, with intra-run CVs of less than 2.9% and inter-run CVs of less than 3.2% for apo(a) molecular weight. This method of molecular weight calibration is applicable to the characterization of any high molecular weight protein that can be successfully electrophoresed, transferred and visualized.
Histatin 3-Mediated Killing of <i>Candida albicans</i> : Effect of Extracellular Salt Concentration on Binding and Internalization (1999)

Human saliva contains histidine-rich proteins, histatins, which have antifungal activity in vitro. The mechanism by which histatins are able to kill Candida albicans may have clinical significance but is currently unknown. Using radiolabeled histatin 3, we show that the protein binds to C. albicans spheroplasts in a manner that is dependent on time and concentration. Binding to the spheroplasts was saturable and could be competed with unlabeled histatin 3. A single histatin 3 binding site with a K(d) = 5.1 microM was detected. Histatin 3 binding resulted in potassium and magnesium efflux, predominantly within the first 30 min of incubation. Studies with fluorescent histatin 3 demonstrate that the protein is internalized by C. albicans and that translocation of histatin inside the cell is closely associated with cell death. Histatin binding, internalization, and cell death are accelerated in low-ionic-strength conditions. Indeed, a low extracellular salt concentration was essential for cell death to occur, even when histatin 3 was already bound to the cell. The interaction of histatin 3 with C. albicans, and subsequent cell death, is inhibited at low temperature. These results demonstrate that the candidacidal activity of histatin 3 is not due exclusively to binding at the cell surface but also involves subsequent interactions with the cell.
New therapies on the horizon for hepatitis C (2003)

Therapy of chronic HCV infection has greatly improved in recent years with the addition of ribavirin to alpha interferon and has further improved more with the use of PEG-interferons. However, more than half of patients do not achieve lasting benefits from these therapies. The future therapeutic developments may include one or more of the following approaches: understanding the HCV genomic organization, elucidating the viral life cycle and HCV replication strategy and understanding the immune mechanisms required for viral propagation or infectivity. The development of novel antiviral strategies and a preventive vaccine against HCV infection remains a major challenge for the future, and will depend on progress on both molecular biology as well as clinical studies. Unfortunately, the low replication of the virus in culture, the lack of convenient animal models, and the high genome variability present mayor challenges for drug development.
Parallel LU factorization for circuit simulation on an MIMD computer (2003)

Direct method circuit simulation on an MIMD (multiple-instruction, multiple-data-stream) machine is studied. The focus is on the parallel LU (lower-upper) factorization a sparse matrix with a nested bordered-block diagonal (BBD) ordering. A novel computation model for the parallel factorization is proposed, and simulation results conducted on a ten-processor Sequent Balance 21000 parallel computer are reported. It is concluded that nested BBD ordering proves to be a highly concurrent structure for parallel LU factorization in direct method circuit simulation.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Pruning the decimation in-time FFT algorithm (1976)

Significant time-saving can be achieved by a simple modification to the radix-2 decimation in-time fast Fourier transform (FFT) algorithm when the data sequence to be transformed contains a large number of zero-valued samples. The time-saving is accomplished by replacing M - L stages of the FFT computation with a simple recopying procedure where 2 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">M</sup> is the total number of points to be transformed of which only 2 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">L</sup> are nonzero.
An Analysis of Experimental Conditions Influencing the Anti‐β<sub>2</sub>‐Glycoprotein I ELISA Assay Results (2007)

Five components of the anti-beta(2)-glycoprotein I (abeta(2)GPI) enzyme-linked immunosorbent assay (ELISA) (coating buffer, microplate brand, blocking buffer, dilution buffer, and conjugate) were analyzed to evaluate how they affect variability in test results. Thirty-two samples from patients with antiphospholipid syndrome (APS) positive for abeta(2)GPI IgG antibodies and three calibrators (a pool of abeta(2)GPI-positive patients, the monoclonal HCAL antibody, and a home-made calibrator) were tested. No differences with regard to the blocking step were noted. Differences were found between the neutral and basic coating buffer when HCAL was used. There were significant differences between Maxisorp and all the other brands of tested microplates. Differences were found between phosphate-buffered saline (PBS) and all the other dilution buffers examined, with exception of TRIS when HCAL or the home-made calibrator was used. There were differences between our routine conjugate and one of the other four conjugates tested when using two of the three calibrators. There were also significant differences between the routine and another conjugate analyzed when using the third calibrator. As variations in abeta(2)GPI ELISA conditions determine significant differences in the results, selecting the appropriate test variables is an important step toward abeta(2)GPI assay standardization.
HERMES: a fast cross-ISA binary translator with post-optimization (2015)

In the era of mobile and cloud computing, cross-ISA (Instruction Set Architecture) binary translation attracts increasing attentions due to the ISA diversity of computing platforms. To easily adapt to vast guest- and host-ISAs with minimal porting efforts, existing cross-ISA binary translators (e.g., QEMU) are typically built upon ISA-independent Intermediate Representation (IR). Although IR conceals the architectural details of different IS As, it also prevents enforcing several effective ISA-specific optimizations, which results in severe performance degradation. To improve the performance of cross-ISA binary translation without loss of portability, we present a fast cross-ISA binary translator, Hermes, by conducting post-optimization on the translated code, rather than on the IR as conventional binary translators do. The proposed post-optimization technique uses Host-specific Data Dependence Graph (HDDG) to significantly eliminate redundant instructions, including arithmetic, load/store and call/return-emulation instructions. To validate our approach, we implement Hermes on a commercial MIPS host system for both ×86 and ARM guest. Compared with QEMU dynamic binary translator, HERMES improves the performance by a factor of 3.14× and 5.18× for ×86 and ARM guest, respectively. Compared with state-of-the-art static binary translator, HERMES achieves comparable performance, while it reduces the translation overhead by 185×.
Topical capsaicin-pharmacology and potential role in the treatment of temporomandibular pain. (1994)

Capsaicin is an over-the-counter topical analgesic marketed at 0.025% and 0.075% concentrations. It is currently approved by the United States Food and Drug Administration for the topical relief of pain due to rheumatoid arthritis, osteoarthritis and various neuralgias. This paper will review the basic and clinical pharmacology of capsaicin, and discuss its potential role in the management of pain in the temporomandibular joint region.
Improvement of Orientation Estimation in Pedestrian Navigation by Compensation of Magnetic Disturbances (2008)

ABSTRACT: Over the last few years there has been a growing interest in the domain of pedestrian navigation, stimulated by the increasing demand for Location Based Services. Pedestrian Dead Reckoning (PDR) based on MEMS sensors has been at the heart of most high-end hybrid solutions devised up to now. In PDR mode, the positioning accuracy is shaped by the individual accuracies in step length and azimuth estimation. This paper will focus on how to improve the latter. Measurements from three single axis MEMS gyroscopes, accelerometers, and magnetometers are merged in an Adaptive Extended Kalman Filter to estimate the absolute orientation of a pedestrian. Enhanced performance is accomplished by the online calibration of the gyroscope and accelerometer biases and through the compensation of magnetic perturbations that disturb the magnetometer measurements. Details of this orientation algorithm and assessment of its effectiveness under different testing conditions are presented in this paper.
Einführung in das Rechnungswesen (1989)

Das Rechnungswesen beinhaltet sämtliche Verfahren zur lückenlosen Erfassung und planmäßigen Ordnung aller Mengen- u. Wertbewegungen in einer Unternehmung. Jede Mengen- u. Wertveränderung wird belegmäßig erfaßt und entsprechend weiterverarbeitet.
Electromagnetic Seed Treatment Increases Germination of Koelreuteria paniculata Laxm.1 (1975)

Abstract Exposure of golden-rain tree seeds to an electromagnetic field of 100 gauss for 4.3 sec partially substitutes for cold stratification if applied after seed scarification. Germination was increased from 56 to 97%, when electromagnetic treatment was applied following scarification and before 84 days of cold stratification.
Magnétorhéomètre à bille (1984)

Nous présentons ici un nouveau rhéomètre qui permet de mesurer : — la viscosité statique d'un fluide viscoélastique avec une précision de 2 % sur une gamme allant de 3 x 10-2 à 105 poises (P), — le module élastique statique d'un solide viscoélastique avec une précision de 10 % sur une gamme allant de 10-2 à 104 dynes/cm2, — le temps de relaxation le plus long d'un corps viscoélastique avec une précision de 10 % pour des temps de relaxation ≳ 4 x 10-2 s.
Optimal ICSI timing after the first polar body extrusion in in vitro matured human oocytes (2007)

Human oocytes matured in vitro needed at least 1 h after first PB extrusion to complete nuclear maturation. Use of a live spindle imaging system can help to decide the timing of ICSI for oocytes matured in vitro.
Plasma polymerization of tetrafluoroethylene. III. Capacitive audio frequency (10 kHz) and AC discharge (1979)

Abstract The plasma polymerization of tetrafluoroethylene (TFE) is studied in a capacitively coupled system with internal electrodes using a 10 kHz (af) and a 60 Hz (ac) source. The emphasis is on identifying conditions that are compatible with continuous coating of plasma polymer on a substrate moving through the center of the interelectrode gap. Operation at a pressure below 100 mTorr is most favorable for deposition of a substantial portion of the plasma polymer on this substrate. Plasma polymer deposited in this way is characterized by ESCA and by deposition rate data and compared to that deposited using rf power in both capacitively and inductively coupled systems. The polymers found in all systems are broadly similar and completely different from conventional poly(TFE). The distribution of power density in the various systems has been identified and compared. This is accomplished by using the known susceptibility of fluorine‐containing polymers (including plasma polymer) to a high‐power plasma as a probe of plasma power density within the interelectrode gap in the capacitively coupled system. The most active zone of the af or ac plasma is close to the electrode at a plasma pressure of approximately 40 mTorr. The use of a magnetic field leads to an intense localized glow such that etching by active fluorine atoms occurs at a specific locus on the electrode. By contrast, the low‐pressure rf capacitively coupled glow discharge is the mildest of those investigated, and its most active zone is further from the electrode and much more diffusely localized by a magnetic field.
Photosensitizer Adhered to Cell Culture Microplates Induces Phototoxicity in Carcinoma Cells (2012)

In vitro experiments in plastic receptacles are the basis of characterization of new photosensitizers (PSs) for the photodynamic therapy. We recently reported that lipophilic PSs adhere to cell culture microplates in a kinetic-like manner (Engelhardt et al., 2011). In the current study, we examined the interaction and phototoxic effects of the microplate-adhered PS in cancer cells. Therefore, we preloaded microplates with hypericin, Foscan, PVP-hypericin, or aluminum (III) phthalocyanine tetrasulfonate chloride (AlPCS4) for 24 hours and measured the PS distribution after addition of A431 human carcinoma cells: following another 24 hours up to 68% of hypericin were detected in the cell fraction. The hydrophilic PVP-hypericin and AlPCS4 also diffused into the cells, but the quantities of PS adherence were considerably lower. Microplate-adhered Foscan appeared not to be redistributed. In contrast to the hydrophilic PSs, the cellular phototoxicity of microplate-adhered lipophilic PS was high, independent of whether the PS (i) was pre-loaded onto microplates or (ii) added simultaneously with the cells or (iii) one day after cell seeding. Based on these results, we suggest testing lipophilic PS dyes for their adherence to microplates. Furthermore, the ability of plastic materials to (reversibly) store PSs might represent a new approach for the PS delivery or the development of antimicrobial coatings.
Comparative Population Regulation of Two Planktonic Cladocera (Holopedium Gibberum and Daphnia Catawba) (1986)

The abundance of two planktonic grazers (Holopedium gibberum and Daphnia catawba) fluctuated seasonally in a nonparallel manner in Lake Lacawac, Pennsylvania. Holopedium gibberum was abundant in early and midsummer, while D. catawba was common only in spring and late summer. I sought to explain this seasonal succession by identifying seasonal factors which regulate each population. I monitored population abundance, individual energy reserves (lipid content), egg production, and body size distributions in plankton samples from April to October in three years. Food resources and vertebrate predator abundance were also examined seasonally. Results of experimental manipulations of food availability and predators, conducted seasonally in both small (6.4—L) and large (6000—L) enclosures, supported interpretations drawn from descriptive data. Contrasting seasonal abundance patterns of the species are explained by differences in the intensity of food stress and predation affecting each species. Food supply restricted population growth of H. gibberum at most times. In the spring of each year, starvation was a major cause of mortality; during summer, individual energy reserves and fecundity were near zero for H. gibberum. Predation losses were negligible for adult H. gibberum, but neonate (smallest age class) mortality by fish was occasionally large. Daphnia catawba experienced a seasonal change in the intensity of regulating factors; food supply restricted population growth only in spring and late summer, but predation controlled growth during summer. During late summer, predation on D. catawba was reduced and its population abundance increased sharply. Experimental removal of fish caused a comparable increase of D. catawba during midsummer. At peak animal densities in late summer, both species were food stressed Life table experiments conducted at this time suggest that competition for food occurs between D. catawba and H. gibberum, and results in a reduction of H. gibberum density.
Familial Tumoral Calcinosis: A Forty-Year Follow-up on One Family (2009)

Familial tumoral calcinosis has a varied natural history; some patients have an unrelenting course, while others may experience quiescent periods. The GALNT3 gene is responsible for the hyperphosphatemic form as seen in this family. Molecular testing may be of benefit to members of affected families, and future studies may help to explain the phenotypic variability among affected individuals. No medical or surgical treatment plan seemed to be effective for controlling the lesions in this family.