gebhart/patrepeval huggingface dataset:
Functional Reconstitution of SdcS, a Na <sup>+</sup> -Coupled Dicarboxylate Carrier Protein from <i>Staphylococcus aureus</i> (2006)

In Staphylococcus aureus, the transport of dicarboxylates is mediated in part by the Na+-linked carrier protein SdcS. This transporter is a member of the divalent-anion/Na+ symporter (DASS) family, a group that includes the mammalian Na+/dicarboxylate cotransporters NaDC1 and NaDC3. In earlier work, we cloned and expressed SdcS in Escherichia coli and found it to have transport properties similar to those of its eukaryotic counterparts (J. A. Hall and A. M. Pajor, J. Bacteriol. 187:5189-5194, 2005). Here, we report the partial purification and subsequent reconstitution of functional SdcS into liposomes. These proteoliposomes exhibited succinate counterflow activity, as well as Na+ electrochemical-gradient-driven transport. Examination of substrate specificity indicated that the minimal requirement necessary for transport was a four-carbon terminal dicarboxylate backbone and that productive substrate-transporter interaction was sensitive to substitutions at the substrate C-2 and C-3 positions. Further analysis established that SdcS facilitates an electroneutral symport reaction having a 2:1 cation/dicarboxylate ratio. This study represents the first characterization of a reconstituted Na+-coupled DASS family member, thus providing an effective method to evaluate functional, as well as structural, aspects of DASS transporters in a system free of the complexities and constraints associated with native membrane environments.
Studies on the Relationship between Morphine Analgesia and the Brain Catecholamines in Mice (1971)

The catecholamine content in the mouse brain has been investigated after the administration of an analgesic dose of morphine. A single dose of 7.5 mg/kg produced a significant reduction in brain noradrenaline during a time‐period of maximal analgesic activity. After inhibition of the rate limiting step of catecholamine synthesis a decrease in noradrenaline content was still obtained. Now even a reduction was found in dopamine content, but this appeared later and was more short‐lasting than the change in noradrenaline. By investigating individual differences in analgesia among animals after inhibition of the catecholamine synthesis or inhibition of dopamine β‐hydroxylation respectively with disulfiram further support was obtained for the view that the release of noradrenaline by morphine is correlated with the analgesic activity better than do changes in dopamine. The possibility that morphine analgesia is induced by interference at the storage sites of brain noradrenaline is discussed.
Allelic Interaction between CRELD1 and VEGFA in the Pathogenesis of Cardiac Atrioventricular Septal Defects (2014)

Atrioventricular septal defects (AVSD) are highly heritable, clinically significant congenital heart malformations. Genetic and environmental modifiers of risk are thought to work in unknown combinations to cause AVSD. Approximately 5-10% of simplex AVSD cases carry a missense mutation in <i>CRELD1</i>. However, <i>CRELD1</i> mutations are not fully penetrant and require interactions with other risk factors to result in AVSD. Vascular endothelial growth factor-A (VEGFA) is a well-characterized modulator of heart valve development. A functional <i>VEGFA</i> polymorphism, <i>VEGFA</i> c.-634C, which causes constitutively increased VEGFA expression, has been associated with cardiac septal defects suggesting it may be a genetic risk factor. To determine if there is an allelic association with AVSD we genotyped the <i>VEGFA</i> c.-634 SNP in a simplex AVSD study cohort. Over-representation of the c.-634C allele in the AVSD group suggested that this genotype may increase risk. Correlation of <i>CRELD1</i> and <i>VEGFA</i> genotypes revealed that potentially pathogenic missense mutations in <i>CRELD1</i> were always accompanied by the <i>VEGFA</i> c.-634C allele in individuals with AVSD suggesting a potentially pathogenic allelic interaction. We used a <i>Creld1</i> knockout mouse model to determine the effect of deficiency of Creld1 combined with increased VEGFA on atrioventricular canal development. Morphogenic response to VEGFA was abnormal in Creld1-deficient embryonic hearts, indicating that interaction between CRELD1 and VEGFA has the potential to alter atrioventricular canal morphogenesis. This supports our hypothesis that an additive effect between missense mutations in <i>CRELD1</i> and a functional SNP in <i>VEGFA</i> contributes to the pathogenesis of AVSD.
Le PAMINSA : Un Nouveau Manipulateur d'Architecture Parallèle aux Mouvements Découplés (2005)

National audience
Abstract #1925: p53-derived peptide targets a novel death pathway inducing selective necrosis in cancer cells (2009)

Introduction: In our preliminary studies we describe p53-derived peptides from the MDM-2 binding domain that possess anti-cancer activity. These molecules appear to promote necrosis of cancer cells while sparing their untransformed counterparts. However, the anti-cancer mechanism remains undefined. To further elucidate this observation we tested PNC-27 which contains the p53 MDM-2 binding domain 12-26 attached to penetratin against different human cancer cell lines. The mechanism of selective cancer cell death was then determined. Methods: Humancancer cell lines (MiaPaCa-2, pancreatic; HT1080, fibrosarcoma; and MCF-7, breast) plus respective untransformed counterparts (HPNE, BJ, and MCF-10) were treated with PNC-27 and control peptide. Peptide mediated necrosis and apoptosis were measured by release of lactate dehydrogenase (LDH) and pro-apoptotic proteins, respectively. Treatment with fluorescent labeled peptide, immunoprecipitation (IP), and time -lapse electron microscopy (EM) were employed to further study the anti-cancer mechanism. Results: Treatment with PNC-27 does not cause elevation of pro-apoptotic proteins (caspases 3,7) but induces early, rapid release of LDH. Accordingly, near 100% tumor cell death was observed. This activity was dose-dependent; an effect not detected in either untransformed or control peptide treated groups. This specific anti-cancer activity, in part, may be due to the observed increased expression of MDM-2 found in cancer cells compared to their normal cellular counterparts which is consistent with the abrogation of MDM-2 and stabilization of p53 protein levels after PNC-27 treatment. Moreover, following IP of fluorescent labeled PNC-27 from treated cancer cells; labeled MDM-2 was also detected. Furthermore, EM revealed the presence of membrane-pore formation and subsequent plasma cell membrane disruption in cancer cells only.Conclusions: These results suggest a mechanism of PNC-27 binding to MDM-2 in the cancer cell/membrane, leading to pore formation and membrane disruption; a p53-independent pathway consistent with rapid selective cancer cell necrosis. Citation Information: In: Proc Am Assoc Cancer Res; 2009 Apr 18-22; Denver, CO. Philadelphia (PA): AACR; 2009. Abstract nr 1925.
Certificate of Advanced Study in Surgical Care Practice (2007)

This course is run by Imperial College School of Medicine, London.
Selenium-catalyzed nonradical chlorination of olefins with N-chlorosuccinimide (1979)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSelenium-catalyzed nonradical chlorination of olefins with N-chlorosuccinimideTetsuo Hori and K. Barry SharplessCite this: J. Org. Chem. 1979, 44, 23, 4204–4208Publication Date (Print):November 1, 1979Publication History Published online1 May 2002Published inissue 1 November 1979https://pubs.acs.org/doi/10.1021/jo01337a046https://doi.org/10.1021/jo01337a046research-articleACS PublicationsRequest reuse permissionsArticle Views1827Altmetric-Citations77LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Mucosal Tolerance to E-selectin and Response to Systemic Inflammation (2006)

Mucosal tolerance to E-selectin has been shown to prevent stroke and reduce brain infarcts in experimental stroke models. However, the effective E-selectin dose range required to achieve mucosal tolerance and the precise mechanisms of neuroprotection remain unclear. We sought to examine the mechanisms of cytoprotection using gene expression profiling of tissues in the setting of mucosal tolerance and inflammatory challenge. Using spontaneously hypertensive rats (SHRs), we achieved immune tolerance with 0.1 to 5 microg E-selectin per nasal instillation and observed a dose-related anti-E-selectin immunoglobulin G antibody production. We also show the distinct patterns of gene expression changes in the brain and spleen with the different tolerizing doses and lipopolysaccharide (LPS) exposure. Prominent differences were seen with such genes as insulin-like growth factors in the brain and downregulation of those encoding the major histocompatibility complex class I molecules in the spleen. In all, mucosal tolerance to E-selectin and subsequent exposure to LPS resulted in significant tissue changes. These changes, while giving an insight to the underlying mechanisms, serve as possible targets for future studies to facilitate translation to human clinical trials.
Performance of the WIYN high-resolution infrared camera (2008)

We present the design overview and on-telescope performance of the WIYN High Resolution Infrared Camera (WHIRC). As a dedicated near-infrared (0.8-2.5 μm) camera on the WIYN Tip-Tilt Module (WTTM), WHIRC will provide near diffraction-limited imaging with a typical FWHM of ~0.25". WHIRC uses a 2048 x 2048 HgCdTe array from Raytheon's VIRGO line, which is a spinoff from the VISTA project. The WHIRC filter complement includes <i>J, H K<sub>S</sub></i>, and 10 narrowband filters. WHIRC's compact design makes it the smallest near-IR camera with this capability. We determine a gain of 3.8 electrons ADU<sup>-1</sup> via a photon transfer analysis and a readout noise of ~27 electrons. A measured dark current of 0.23 electrons s<sup>-1</sup> indicates that the cryostat is extremely light tight. A plate scale of 0.098" pixel<sup>-1</sup> results in a field of view (FOV) of ~3' x 3', which is a compromise between the highest angular resolution achievable and the largest FOV correctable by WTTM. Measured throughput values (~0.33 in <i>H</i>-band) are consistent with those predicted for WHIRC based on an elemental analysis. WHIRC was delivered to WIYN in July 2007 and was opened for shared risk use in Spring 2008. WHIRC will be a facility instrument at the WIYN telescope enabling high definition near-infrared imaging studies of a wide range of astronomical phenomena including star formation regions, proto-planetary disks, stellar populations and interstellar medium in nearby galaxies, and supernova and gamma-ray burst searches.
ChemInform Abstract: An Improved Asymmetric Nitroolefination of α‐Alkyl‐γ‐ and . delta.‐lactones with Modified Nitroenamines. (1995)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Fractional Vortices and Composite Domain Walls in Flat Nanomagnets (2005)

We provide a simple explanation of complex magnetic patterns observed in ferromagnetic nanostructures. To this end we identify elementary topological defects in the field of magnetization: ordinary vortices in the bulk and vortices with half-integer winding numbers confined to the edge. Domain walls found in experiments and numerical simulations in strips and rings are composite objects containing two or more of the elementary defects.
Effects of <i>β</i>-xylosides on proteoglycan biosynthesis and morphology of PC12 pheochromocytoma cells and primary cultures of rat cerebellum (1991)

We have examined the effects of beta-xylosides, which act as exogenous acceptors for glycosaminoglycan chain initiation, on the morphology and proteoglycan biosynthesis of PC12 pheochromocytoma cells, and on monolayer, aggregate and explant cultures of early postnatal rat cerebellum. PC12 cells cultured for 13 days in the presence of nerve growth factor (NGF) and beta-xyloside, and labeled during days 11-13 with sodium [35S]sulfate, showed an 8- to 11-fold increase in [35S]sulfate-labeled macromolecules released into the culture medium. Most of the increase was accounted for by chondroitin sulfate, which was in the form of free glycosaminoglycan chains, which were not acid-precipitable. The presence of beta-xyloside also led to a 65-115% increase in [35S]sulfate incorporation into cell-associated glycosaminoglycans and glycoproteins of untreated and NGF-treated PC12 cells, respectively. beta-Xyloside treatment reduced the size of the chondroitin sulfate chains in both the cells and medium from approximately 34,000 to 10,000 Mr, but had much less effect on heparan sulfate, which decreased in size from 16,000 to 13,000-14,500 Mr (in the medium and cells, respectively). beta-Xyloside inhibition of proteoglycan biosynthesis was accompanied by significant morphological effects in NGF-treated PC12 cells, consisting of an increase in length and decrease in the branching, diameter and adhesion to the collagen substratum of the PC12 cell processes. p-Nitrophenyl- and 4-methylumbelliferyl-beta-D-xylosides produced similar effects, which were not seen with p-nitrophenyl-beta-D-galactoside. beta-Xylosides also produced distinct alterations in the adhesion and morphology of monolayer, aggregate, and explant cultures of early postnatal rat cerebellum, which occurred together with inhibition of chondroitin sulfate proteoglycan biosynthesis and a decrease in glycosaminoglycan chain size. These studies indicate that chondroitin sulfate (and probably also heparan sulfate) proteoglycans play a significant role in modulating cell-cell and cell-matrix interactions in nervous tissue development and differentiation.
TCP Smart-Framing: using smart segments to enhance the performance of TCP (2002)

In this paper we propose an enhancement to the TCP protocol, called TCP Smart-Framing(TCP-SF), that enables the Fast Recovery algorithm for short lived flows, as most of the current Internet traffic is. Without modifying the TCP congestion control based on the additive-increase/multiplicative-decrease paradigm, TCP Smart-Framing adopts a novel segmentation algorithm: while classic TCP starts sending one segment, a TCP-SF source is allowed to send an initial window of 4 smaller segments, whose aggregate payload is equal to the connection's MSS. This key idea can be implemented on top of any TCP flavor, from Tahoe to SACK, and requires modifications to the server behavior only.
Notes - Oxidation of 3-Methylisoquinoline (1958)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNotes - Oxidation of 3-MethylisoquinolineHenry Baumgarten and Jerald DirksCite this: J. Org. Chem. 1958, 23, 6, 900–902Publication Date (Print):June 1, 1958Publication History Published online27 May 2003Published inissue 1 June 1958https://pubs.acs.org/doi/10.1021/jo01100a604https://doi.org/10.1021/jo01100a604research-articleACS PublicationsRequest reuse permissionsArticle Views145Altmetric-Citations5LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Exponential Sums Over Primes in an Arithmetic Progression (1985)

In 1979 A. F. Lavrik obtained some estimates for exponential sums over primes in arithmetic progressions by an analytic method. In the present paper we give an estimate for the same sums, comparable with Lavrikâs estimate, by means of elementary methods like Vaughanâs identity.
ChemInform Abstract: High Diastereoselection in the Intramolecular Diels‐Alder Reaction of o‐Quinodimethanes: An Expedient Entry to trans‐Benzoperhydrindans. A Highly Stereoselective Total Synthesis of (.+‐.)‐Estrone and (.+‐.)‐Adrenosterone. (1989)

Abstract The dioxolane (I) is transformed to the trans‐benzoperhydrindan (II) via an intramolecular Diels‐Alder reaction.
HOW WE SOLVED THE $100,000 DECIPHER PUZZLE (16 HOURS TOO LATE) (1990)

On Saturday March 30, 1985, we solved a cryptography puzzle that had remained unsolved for over two years. Unfortunately for us, we missed by one day the deadline for sharing what had become a $117,000 prize. This paper describes the puzzle and how we solved it. The $100,000 Decipher Puzzle consists of a simple two-sided jigsaw puzzle that contains a sequence of 376 codenumbers, ranging from 1 to 1252, which were encrypted by a multiple-substitution cipher similar to the Beale cipher. Clues state that the key was derived from some keytext in the public domain. We solved the puzzle by building a Zetalisp program that tested candidate keytexts by trying a variety of ways to extract candidate keys from each keytext and by checking the resulting candidate plaintexts for English. Our program exploited a novel “windowing” technique that detected when part of a candidate key was correct and an effective test for English based on digraph frequencies.
Effect of Ions on Filtration Rates Through Cellophane Membranes. (1932)

When distilled water is filtered through cellophane membranes which have been evacuated prior to several days' soaking in water, it is found that the rate of filtration usually decreases during the first hour, finally reaching an essentially constant value. We predicted that it should be possible to raise or lower the value of this final “steady” rate of filtration by adding varying amounts of ThCl4 to the water. The preliminary results given below show that the steady rate of filtration is affected by ThCl4. The membrane was placed between glass cylinders and supported by a coarse alundum disc. Filtration was carried out at 10 cm. Hg. The rate of filtration was determined by following through a microscope equipped with a micrometer eye-piece the displacement of the meniscus in a capillary. A solution of 10-3 gms. ThCl4 per liter gave a 25% increase in the rate of filtration over that of distilled water. Solutions of 1 gm. and of 10 gm. ThCl4 per liter showed a decrease in the rate of filtration of 29% (average of 5 determinations) and 35% (average of 3 determinations), respectively. A solution of 10-2 gm. ThCl4 per liter showed no large effect. A hypothesis concerning the mechanism of these processes, together with an extension of experimental results, will appear in a subsequent communication.
Studies on Coördination Compounds. VIII. Some Factors Concerning the Effect of the Terminal Groups on the Chelating Abilities of β-Diketones (1953)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStudies on Coördination Compounds. VIII. Some Factors Concerning the Effect of the Terminal Groups on the Chelating Abilities of β-DiketonesLeGrand G. Van Uitert and W. Conard FerneliusCite this: J. Am. Chem. Soc. 1953, 75, 15, 3862–3864Publication Date (Print):August 1, 1953Publication History Published online1 May 2002Published inissue 1 August 1953https://doi.org/10.1021/ja01111a528RIGHTS & PERMISSIONSArticle Views73Altmetric-Citations26LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (368 KB) Get e-Alerts Get e-Alerts
Initial Oxidation of a Rh(110) Surface Using Atomic or Molecular Oxygen and Reduction of the Surface Oxide by Hydrogen (2005)

The formation conditions, morphology, and reactivity of thin oxide films, grown on a Rh(110) surface in the ambient of atomic or molecular oxygen, have been studied by means of laterally resolved core level spectroscopy, scanning tunneling microscopy and low energy electron diffraction. Exposures of Rh(110) to atomic oxygen lead to subsurface incorporation of oxygen even at room temperature and facile formation of an ordered, laterally uniform surface oxide at approximately 520 K, with a quasi-hexagonal structure and stoichiometry close to that of RhO(2). In the intermediate oxidation stages, the surface oxide coexists with areas of high coverage adsorption phases. After a long induction period, the reduction of the Rh oxide film with H(2) is very rapid and independent of the coexisting adsorption phases. The growth of the oxide film by exposure of a Rh(110) surface to molecular oxygen requires higher pressures and temperatures. The important role of the O(2) dissociation step in the oxidation process is reflected by the complex morphology of the oxide films grown in O(2) ambient, consisting of microscopic patches of different Rh and oxygen atomic density.
Placing and positioning technique of blocks for gravity wharf (2009)

Based on the comparison of several positioning methods for blocks' placing for a gravity wharf,we put forward the constructive proposal which combines the measurement frame positioning with extension line positioning,and which is beneficial to the raise of blocks' placement and guarantee of erecting quality.