gebhart/patrepeval huggingface dataset:
Review of The Long, Slow Death of White Australia by Gwenda Tavan, Carlton North: Scribe Publications, 2005, 298 Pages, Paperback, $32.95 (2005)

Eukaryotic cells limit ribosomal DNA (rDNA) transcription by RNA polymerase I (RNAP-I) to maintain genome integrity. African trypanosomes present an excellent model for studies on RNAP-I regulation because they possess a bifunctional RNAP-I and because RNAP-II transcription appears unregulated. Since Elp3, the catalytic component of Elongator, controls RNAP-II transcription in yeast and human cells, we predicted a role for a trypanosome Elp3-related protein, ELP3a or ELP3b, in RNAP-I regulation. elp3b null and conditional strains specifically exhibited resistance to a transcription elongation inhibitor, suggesting that ELP3b negatively impacts elongation. Nascent RNA analysis and expression of integrated reporter cassettes supported this interpretation and revealed negative control of rDNA transcription. ELP3b specifically localized to the nucleolus, and ELP3b loss rendered cells hypersensitive to DNA damage and to translation inhibition, suggesting that anti-Elongator function was important to maintain genome integrity rather than to modulate ribosome production. Finally, ELP3b displayed discrimination between RNAP-I compartments in the same cell. Our results establish ELP3b as a major negative regulator of rDNA transcription and extend the roles of the Elp3-related proteins to RNAP-I transcription units. ELP3b is also the first trypanosome protein shown to distinguish between rDNA and variant surface glycoprotein transcription within different RNAP-I compartments.
Epitope Tagging (2008)

Epitope tagging is widely used in the characterization of newly discovered proteins. This review presents an overview of how the technique evolved and how it is being used today, with a focus on its use in the study of protein-protein interactions. In addition, the evolution of the technique for proteomic analyses is described.
Development of Star-Shaped Four-Channel CWDM Optical-Subassembly Module (2005)

We developed an optical sub-assembly (OSA) module for a four-channel coarse wavelength division multiplexing (CWDM) transceiver that satisfied the size requirement for XENPAK, X2 and XPAK multisource agreement (MSA). This module is composed of a frame structure with compact star-shaped optics paths, die-on-header laser or photodetector, thin film filters, ball lens and SC receptacle. The star-shaped optics design minimizes the reflective surfaces and makes the optics path shortest by using the concept of die-on-headers that are similar to To-can packages. The maximal optics path is 17.25mm from the laser diode to the fiber and the minimum is 12.00mm in the transmitter of optical subassembly (TOSA). In the receiver of optical subassembly (ROSA), the maximal optics path is 17.98mm from the fiber to photodetector and the minimum is 12.73mm. The designs of the die-on-header laser or photodetector and the frame structure provide both the passive and the active alignment versatilities. By the passive alignment method, we achieved the optimal optical condition for each channel in which the wavelengths varied from 1275nm to 1350nm. Furthermore, by using the active alignment method in the last stage of the assembly process, we compensated inaccuracies in the assembly process. As a result, the component assembly tolerance range of the OSA module increased up to 18-folds. Besides, the concept of hermetic sealing can also be executed in this design. From the analysis, it was found that the developed OSA module, no matter it is made of tool steel or optics plastic, shows good thermal stability for coupling efficiency when temperature changes from room temperature to -40degC and 85degC
Algorithms for Model-Based Gaussian Hierarchical Clustering (1998)

Agglomerative hierarchical clustering methods based on Gaussian probability models have recently shown promise in a variety of applications. In this approach, a maximum-likelihood pair of clusters is chosen for merging at each stage. Unlike classical methods, model-based methods reduce to a recurrence relation only in the simplest case, which corresponds to the classical sum of squares method. We show how the structure of the Gaussian model can be exploited to yield efficient algorithms for agglomerative hierarchical clustering.
Cyp26 enzymes are required to balance the cardiac and vascular lineages within the anterior lateral plate mesoderm (2014)

Normal heart development requires appropriate levels of retinoic acid (RA) signaling. RA levels in embryos are dampened by Cyp26 enzymes, which metabolize RA into easily degraded derivatives. Loss of Cyp26 function in humans is associated with numerous developmental syndromes that include cardiovascular defects. Although previous studies have shown that Cyp26-deficient vertebrate models also have cardiovascular defects, the mechanisms underlying these defects are not understood. Here, we found that in zebrafish, two Cyp26 enzymes, Cyp26a1 and Cyp26c1, are expressed in the anterior lateral plate mesoderm (ALPM) and predominantly overlap with vascular progenitors (VPs). Although singular knockdown of Cyp26a1 or Cyp26c1 does not overtly affect cardiovascular development, double Cyp26a1 and Cyp26c1 (referred to here as Cyp26)-deficient embryos have increased atrial cells and reduced cranial vasculature cells. Examining the ALPM using lineage tracing indicated that in Cyp26-deficient embryos the myocardial progenitor field contains excess atrial progenitors and is shifted anteriorly into a region that normally solely gives rise to VPs. Although Cyp26 expression partially overlaps with VPs in the ALPM, we found that Cyp26 enzymes largely act cell non-autonomously to promote appropriate cardiovascular development. Our results suggest that localized expression of Cyp26 enzymes cell non-autonomously defines the boundaries between the cardiac and VP fields within the ALPM through regulating RA levels, which ensures a proper balance of myocardial and endothelial lineages. Our study provides novel insight into the earliest consequences of Cyp26 deficiency that underlie cardiovascular malformations in vertebrate embryos.
Time-Delay And Doppler-Shift Based Geolocation By Semi-Definite Programming (2012)

Publication in the conference proceedings of EUSIPCO, Bucharest, Romania, 2012
Factors associated with parameters of engraftment potential of umbilical cord blood (2006)

UCB banks should emphasize selecting the heaviest infants and processing large-volume units with high NCCs to optimize hematopoietic potential. Minority recruitment should be encouraged with consideration given to inherent racial differences in cell counts. There does not appear to be a significant relationship between other neonatal and parental characteristics and that of engraftment potential.
α-Crystallin Chaperone-like Activity and Membrane Binding in Age-Related Cataracts (2001)

alpha-Crystallin, the major protein component of the vertebrate lens, is thought to play a critical role in the maintenance of transparency through its ability to inhibit stress-induced protein aggregation. However, during aging and cataract formation the amount of membrane-bound alpha-crystallin increases significantly while high molecular weight complexes (HMWCs) comprised of alpha-crystallin and other lens crystallins accumulate. These and other recent data suggest a possible link between cataract formation, the formation of high molecular weight alpha-crystallin aggregates, and the progressive increase in membrane association of alpha-crystallin. To better understand these processes, we characterized the chaperone-like activity (CLA) and subunit exchange of membrane bound alpha-crystallin. In addition, we measured the membrane binding properties of in vitro constituted HMWCs to understand the mechanism by which increased alpha-crystallin is bound to the membrane of old and cataractous lens cells in vivo. Membrane-associated alpha-crystallin complexes have measurably reduced CLA compared to complexes in solution; however, membrane binding does not alter the time required for alpha-crystallin complexes to reach subunit exchange equilibrium. In addition, HMWCs prepared in vitro have a profoundly increased membrane binding capacity as compared to native alpha-crystallin. These results are consistent with a model in which increased membrane binding of alpha-crystallin is an integral step in the pathogenesis of many forms of cataracts.
Local Generation of C-Reactive Protein in Diseased Coronary Artery Venous Bypass Grafts and Normal Vascular Tissue (2003)

Background— Venous coronary artery bypass grafts (CABGs) are prone to accelerated atherosclerosis. In atherosclerotic diseases, serum C-reactive protein (CRP) levels have become an important diagnostic and prognostic marker. The origin of CRP in this setting remains to be elucidated. Methods and Results— Monoclonal anti-CRP identified CRP expression in medial and intimal α-actin–positive smooth muscle cells (SMCs) of diseased CABGs with type V and VI lesions and also of native saphenous veins of atherosclerotic individuals. In addition, patent coronary arteries with type IV and V but not with type I through III lesions exhibited intense SMC staining for CRP. Calcified desobliterates of occluded coronary arteries with end-stage disease did not show SMC staining for CRP and were consistently negative for CRP mRNA, as detected by means of real-time polymerase chain reaction. However, CRP mRNA was expressed in 11 of 15 diseased CABGs and also in 10 of 15 native veins. By contrast, only 3 of 18 internal mammary and 4 of 12 radial arteries with virtually no atherosclerosis were positive for CRP mRNA. Conclusions— CRP is produced by SMCs of atherosclerotic lesions with active disease but not in end-stage plaques. The role of CRP constitutively expressed by normal vascular tissue in vein graft disease has yet to be elucidated.
Acute Effects of Ghrelin on Insulin Secretion and Glucose Disposal Rate in Gastrectomized Patients (2006)

It appears that ghrelin might be involved in the negative control of insulin secretion and glucose consumption in gastrectomized patients, at least after acute administration.
Non-Oxidative Coupling of Methane to C<sub>2</sub> Hydrocarbons under Above-Atmospheric Pressure Using Pulsed Microwave Plasma (2002)

A conventional high voltage wire-like plasma can be enhanced and spread by pulsed microwave to form an umbrella-like plasma which is continuously distributed in a reactor. Using such enhanced plasma, methane was directly converted to C2 hydrocarbons with higher yield than using conventional plasma at atmospheric or higher pressure. There was hardly any detectable trace of ethane in the product. The effects of pressure, microwave power, pulse duration, H2/CH4 mole ratio, and flow rate on methane conversion were investigated. With flow rate of 300 mL/min, H2/CH4 ratio of 2, pressure of 0.13MPa, peak microwave power of 120 W, pulse duty factor of 400/400 ms, conversion of methane and yield of C2 had reached up to 59.2% and 52.1%, respectively. The ratio of C2H2/C2H4 in the product could be adjusted by changing the pressure.
Verlorene Wandteppiche und politische Symbolik. (1997)

ZusammenfassungIm Spätsommer des Jahres 1513 traf Margarete, Regentin der Niederlande, Erzherzogin von Österreich, Herzogin von Burgund und Herzoginwitwe von Savoyen, in der Bischofsstadt Tournai ein. Sie war gekommen, um Heinrich VIII. von England zu treffen und einen Sieg zu feiern: Der junge englische König und ihr Vater, Maximilian, Kaiser von Österreich, hatten gemeinsam die französischen Herrscher von Flämisch-Burgund besiegt. Die Bürger von Tournai, die ihre illustren Gäste jubelnd begrüßten, hatten ihre Stadt mit leuchtenden Wandteppichen, ihrem berühmtesten Erzeugnis, geschmückt. Margarete zog in Tournai als eine starke und selbstbewußte dreiunddreißigjährige Frau ein. Im Alter von einundzwanzig war sie bereits dreimal als Schachfigur auf dem königlichen Heiratsmarkt vermählt worden. Sie hatte jedoch im Jahre 1506 eine vierte Heirat mit dem ältlichen, verwitweten Heinrich VII. von England abgelehnt und beharrte von da an auf ihrer Rolle als Witwe und Regentin für ihren Neffen, den späteren Kaiser Karl V.
Design of multiple band-notch using meander lines for compact ultra-wide band antennas (2012)

In this study, the design of multiple band notch for compact ultra-wide band (UWB) antennas is proposed. The antenna consists of an elliptical radiator, a microstrip-feed line and a ground plane. Four pairs of meander lines (MLs), working as resonators, are added to the antenna to produce a quadruple band notch characteristic, yet without increasing the overall antenna size. Two types of feeding techniques, direct-connected feed and parallel-coupled feed, are used in the resonators. The centre frequencies and bandwidths of the individual notches can be adjusted independently using the dimensions of the corresponding MLs. For verification, the proposed antenna is also fabricated and measured. The return loss, peak gain, efficiency and radiation pattern of the band-notch antenna are studied using both computer simulation and measurement. The pulse responses of the band-notched antennas are also measured and compared with those of single-, double- and triple-band-notch antennas.
Face obscuration in a video sequence by integrating kernel-based mean-shift and active contour (2008)

A technology for protecting privacy in video surveillance is presented in this paper. Human identity that can contain privacy intrusive information is protected. By integrating mean-shift and active contour, faces can be tracked and blurred in each frame of a video sequence. In the initial frame, faces are located by a face detector. We extend the Adaboost multiview face detector to detect the low-resolution faces. In order to improve the efficiency of the detection and tracking, the background subtraction is used to constrain the face search region. The face is modeled as an ellipse and the centre of the ellipse is predicted using mean shift. The position and scale of the mean shift are updated using the active contour. The combined mean shift and active contour improves the robustness of the tracking. Experimental results show that the algorithm is robust to occlusion and scale variation.
Modeling the responsivity and self-emission of a double-beam Fourier-transform infrared interferometer (1999)

A modeling study aimed at characterizing the radiometric properties of a double-beam Fourier-transform infrared interferometer is presented. Measurements showed that the two responsivities associated with each interferometer channel are different in certain spectral regions. This anomaly was attributed to a dissymmetry between the optical transmissions of the two plates that form the beam splitter. This dissymmetry is primarily responsible for the instrument residual emission. A secondary cause of residual emission is attributed to the relative alignment of the two input optics. Both effects were taken into account in a model that gives the instrument residual emission in terms of the beam splitter temperature. Actual results indicate that in the 7-14-microm window the instrument residual emission can be modeled with an absolute radiometric error smaller than 0.5 K (blackbody at 290 K). The model was used to develop an automatic calibration procedure that yields radiance errors smaller than 0.05 microW/cm(2) sr cm(-1) in the 7-14-microm band. The radiometric stability of the interferometer was analyzed.
Heterocyclic Sulfonamides as Carbonic Anhydrase Inhibitors<sup>1</sup> (1950)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTHeterocyclic Sulfonamides as Carbonic Anhydrase Inhibitors1Wilbur H. Miller, Alice M. Dessert, and Richard O. Roblin Jr.Cite this: J. Am. Chem. Soc. 1950, 72, 11, 4893–4896Publication Date (Print):November 1, 1950Publication History Published online1 May 2002Published inissue 1 November 1950https://pubs.acs.org/doi/10.1021/ja01167a012https://doi.org/10.1021/ja01167a012research-articleACS PublicationsRequest reuse permissionsArticle Views438Altmetric-Citations145LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Clifford Algebras, Spin Groups and Qubit Trees (2022)

Representations of Spin groups and Clifford algebras derived from the structure of qubit trees are introduced in this work. For ternary trees the construction is more general and reduction to binary trees is formally defined by deletion of superfluous branches. The usual Jordan–Wigner construction also may be formally obtained in this approach by bringing the process up to trivial qubit chain (trunk). The methods can also be used for effective simulation of some quantum circuits corresponding to the binary tree structure. The modeling of more general qubit trees, as well as the relationship with the mapping used in the Bravyi–Kitaev transformation, are also briefly discussed. Quanta 2022; 11: 97–114.
Teaching new subjects? The hidden curriculum of marketised education systems (1996)

Many countries have introduced a range of policies that attempt to reformulate the relationships among government, schools, and parents through the application of market forces. This paper looks at the curriculum of marketization and explores the extent to which the recent trend toward quasi-markets in public education systems are permeating the classroom and affecting the nature of educational transmissions. The paper looks at research from England, Australia, New Zealand, and the United States and explores the tension between the overt and hidden curriculum of the reforms and its significance for fostering different forms of social solidarity. The paper also connects with discussions on the globalization of education policy and/or broader changes in the nature of modern/postmodern societies, and the role of national education systems in encouraging and/or inhibiting such developments. (Contains 54 references.) (LMI) ******************************************************************************** Reproductions supplied by EDRS are the best that can be made from the original document. ******************************************************************************** Teaching New Subjects? The Hidden Curriculum of Marketized Education Systems Sally Power School of Education University of Bristol, UK
Effective treatment of severe COVID-19 patients with tocilizumab (2020)

After analyzing the immune characteristics of patients with severe coronavirus disease 2019 (COVID-19), we have identified that pathogenic T cells and inflammatory monocytes with large amount of interleukin 6 secreting may incite the inflammatory storm, which may potentially be curbed through monoclonal antibody that targets the IL-6 pathways. Here, we aimed to assess the efficacy of tocilizumab in severe patients with COVID-19 and seek a therapeutic strategy. The patients diagnosed as severe or critical COVID-19 in The First Affiliated Hospital of University of Science and Technology of China (Anhui Provincial Hospital) and Anhui Fuyang Second People's Hospital were given tocilizumab in addition to routine therapy between 5 and 14 February 2020. The changes of clinical manifestations, computerized tomography (CT) scan image, and laboratory examinations were retrospectively analyzed. Fever returned to normal on the first day, and other symptoms improved remarkably within a few days. Within 5 d after tocilizumab, 15 of the 20 patients (75.0%) had lowered their oxygen intake, and 1 patient needed no oxygen therapy. CT scans manifested that the lung lesion opacity absorbed in 19 patients (90.5%). The percentage of lymphocytes in peripheral blood, which decreased in 85.0% of patients (17/20) before treatment (mean, 15.52 ± 8.89%), returned to normal in 52.6% of patients (10/19) on the fifth day after treatment. Abnormally elevated C-reactive protein decreased significantly in 84.2% of patients (16/19). No obvious adverse reactions were observed. All patients have been discharged on average 15.1 d after giving tocilizumab. Preliminary data show that tocilizumab, which improved the clinical outcome immediately in severe and critical COVID-19 patients, is an effective treatment to reduce mortality.
Reactivation of maternal SNORD116 cluster via SETDB1 knockdown in Prader-Willi syndrome iPSCs (2014)

Prader-Willi syndrome (PWS), a disorder of genomic imprinting, is characterized by neonatal hypotonia, hypogonadism, small hands and feet, hyperphagia and obesity in adulthood. PWS results from the loss of paternal copies of the cluster of SNORD116 C/D box snoRNAs and their host transcript, 116HG, on human chromosome 15q11-q13. We have investigated the mechanism of repression of the maternal SNORD116 cluster and 116HG. Here, we report that the zinc-finger protein ZNF274, in association with the histone H3 lysine 9 (H3K9) methyltransferase SETDB1, is part of a complex that binds to the silent maternal but not the active paternal alleles. Knockdown of SETDB1 in PWS-specific induced pluripotent cells (iPSCs) causes a decrease in the accumulation of H3K9 trimethylation (H3K9me3) at 116HG and corresponding accumulation of the active chromatin mark histone H3 lysine 4 dimethylation (H3K4me2). We also show that upon knockdown of SETDB1 in PWS-specific iPSCs, expression of maternally silenced 116HG RNA is partially restored. SETDB1 knockdown in PWS iPSCs also disrupts DNA methylation at the PWS-IC where a decrease in 5-methylcytosine is observed in association with a concomitant increase in 5-hydroxymethylcytosine. This observation suggests that the ZNF274/SETDB1 complex bound to the SNORD116 cluster may protect the PWS-IC from DNA demethylation during early development. Our findings reveal novel epigenetic mechanisms that function to repress the maternal 15q11-q13 region.
[Decubitus sores in geriatric medicine. Local and general treatment of pressure sores in the aged]. (1999)

Surgical repair with flaps or grafts is not usually indicated in fragile elderly patients. Special attention must be given to all associated diseases (diabetes, heart failure, etc.) as well as protein-calorie nutrition. The objective is 35 cal/kg/d including 1.25-1.5 g protein/kg/d. TREATMENT OF COMPLICATIONS: Wound infection should be suspected in case of an inflammatory induration around the wound, fever, or delayed healing. Systemic antibiotics are indicated as for cellulitis. The wound is generally colonized by germs found on local bacteriological samples. Osteitis should be suspected if the wound is in contact with bone. In case of biopsy proven osteitis with positive culture, prolonged oral antibiotics are indicated. Tendon retractions should benefit from rehabilitation exercises and surgery in case of medical failure. Antalgesics may be required, either continuously or when making the dressings.