gebhart/patrepeval huggingface dataset:
Escherichia coli SecB stimulates export without maintaining export competence of ribose-binding protein signal sequence mutants (1996)

Ribose-binding protein (RBP) is exported to the periplasm of Escherichia coli via the general export pathway. An rbsB-lacZ gene fusion was constructed and used to select mutants defective in RBP export. The spontaneous Lac+ mutants isolated in this selection contained either single-amino-acid substitutions or a deletion of the RBP signal sequence. Intact rbsB genes containing eight different point mutations in the signal sequence were reconstructed, and the effects of the mutations on RBP export were examined. Most of the mutations caused severe defects in RBP export. In addition, different suppressor mutations in SecY/PrlA protein were analyzed for their effects on the export of RBP signal sequence mutants in the presence or absence of SecB. Several RBP signal sequence mutants were efficiently suppressed, but others were not suppressed. Export of an RBP signal sequence mutant in prlA mutant strains was partially dependent on SecB, which is in contrast to the SecB independence of wild-type RBP export. However, the kinetics of export of an RBP signal sequence mutant point to a rapid loss of pre-RBP export competence, which occurs in strains containing or lacking SecB. These results suggest that SecB does not stabilize the export-competent conformation of RBP and may affect translocation by stabilizing the binding of pre-RBP at the translocation site.
Growth and Characterization of Vertical and Lateral p‐n Junctions Formed by Selective‐Area p‐GaN MOVPE on Patterned Templates (2018)

In this work, vertical and lateral GaN p‐n junctions are investigated. In particular, there is a strong demand of lateral p‐n junctions for the realization of numerous types of power devices. The comparison of I‐V characteristics of regrown and continuously‐grown p‐n diodes, formed by metal‐organic vapor phase epitaxy (MOVPE), reveals the impact of different surface treatments before regrowth. The use of tetramethylammonium hydroxide (TMAH) prove vital for growth on etch‐damaged GaN and to smoothen vertical sidewalls for selective‐area regrowth (SAR). Initial growth on patterned templates is identified to occur both vertically on the horizontal c‐plane and laterally on the vertical m‐planes allowing for an integration of the developed process in power device fabrication routes. The formation of local superelevations (LSE) near edges of masked areas is analyzed and explained.
Sunlight, Vitamin D, and Ovarian Cancer Mortality Rates in US Women (1994)

This ecologic study supports the hypothesis that sunlight may be a protective factor for ovarian cancer mortality.
Wireless Resource Scheduling Based on Backoff for Multiuser Multiservice Mobile Cloud Computing (2016)

Mobile cloud computing (MCC) can significantly improve the processing/storage capacity and standby time of mobile terminals by migrating data processing and storage to the remote cloud. However, due to the wireless resource limitations of access points/base stations, data streaming of MCC suffers poor quality-of-service (QoS) in multiuser multiservice scenarios, such as long buffering time and intermittent disruptions. In this paper, we propose a backoff-based wireless resource scheduling (BWRS) scheme, in which real-time services have higher priority than non-real-time services. BWRS can improve the QoS of real-time streams and the overall performance of MCC networks. We formulate an M/M/1 queueing model and propose a queueing-delay-optimal control algorithm to minimize the average queueing delay of non-real-time services. Furthermore, a delay-constrained control algorithm is developed not only to minimize the queueing delay of non-real-time services of multiservice users but to support users' non-real-time services under delay constraints as well. The simulation results show that the proposed scheme can minimize the average queueing delay while still meeting the delay requirement and can significantly improve blocking probability and channel utilization.
Re‐thinking miRNA‐mRNA interactions: Intertwining issues confound target discovery (2015)

Despite a library full of literature on miRNA biology, core issues relating to miRNA target detection, biological effect, and mode of action remain controversial. This essay proposes that the predominant mechanism of direct miRNA action is translational inhibition, whereas the bulk of miRNA effects are mRNA based. It explores several issues confounding miRNA target detection, and discusses their impact on the dominance of "miRNA seed" dogma and the exploration of non-canonical binding sites. Finally, it makes comparisons between miRNA target prediction and transcription factor binding prediction, and questions the value of characterizing miRNA binding sites based on which miRNA nucleotides are paired with an mRNA.
The soluble mammalian sperm factor protein that triggers Ca<sup>2+</sup> oscillations in eggs: Evidence for expression of mRNA(s) coding for sperm factor protein(s) in spermatogenic cells (2000)

At fertilisation in mammals the sperm initiates a series of Ca2+ oscillations that activate development. One theory of signalling at fertilisation suggests that the sperm contains a soluble protein factor that causes these Ca2+ oscillations by entering the egg after sperm-egg membrane fusion. This theory is supported by the finding that, in some species, injection of sperm protein extracts into eggs triggers a pattern of Ca2+ oscillations similar to those seen at fertilisation. So far, all the direct evidence for a sperm factor has been based upon the injection of soluble proteins from mature sperm. Here, we demonstrate that injection of mRNA extracted from hamster spermatogenic cells also leads to generation of prolonged Ca2+ oscillations in mouse eggs. The ability of spermatogenic cell mRNA to induce Ca2+ oscillations is dependent upon translation into protein and also appears to be specific to spermatogenic cells since injection of mRNA isolated from somatic tissues into eggs was ineffective. These data support the hypothesis that sperm contain a soluble, cytosolic protein factor that induces Ca2+ oscillations in eggs at fertilisation. These data are discussed in the light of our recent findings that suggest that the sperm factor possesses a phospholipase C activity.
Heterogeneous Nuclear Ribonucleoprotein A1 Binds to the 3′-Untranslated Region and Mediates Potential 5′-3′-End Cross Talks of Mouse Hepatitis Virus RNA (2001)

The 3'-untranslated region (3'-UTR) of mouse hepatitis virus (MHV) RNA regulates the replication of and transcription from the viral RNA. Several host cell proteins have previously been shown to interact with this regulatory region. By immunoprecipitation of UV-cross-linked cellular proteins and in vitro binding of the recombinant protein, we have identified the major RNA-binding protein species as heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1). A strong hnRNP A1-binding site was located 90 to 170 nucleotides from the 3' end of MHV RNA, and a weak binding site was mapped at nucleotides 260 to 350 from the 3' end. These binding sites are complementary to the sites on the negative-strand RNA that bind another cellular protein, polypyrimidine tract-binding protein (PTB). Mutations that affect PTB binding to the negative strand of the 3'-UTR also inhibited hnRNP A1 binding on the positive strand, indicating a possible relationship between these two proteins. Defective-interfering RNAs containing a mutated hnRNP A1-binding site have reduced RNA transcription and replication activities. Furthermore, hnRNP A1 and PTB, both of which also bind to the complementary strands at the 5' end of MHV RNA, together mediate the formation of an RNP complex involving the 5'- and 3'-end fragments of MHV RNA in vitro. These studies suggest that hnRNP A1-PTB interactions provide a molecular mechanism for potential 5'-3' cross talks in MHV RNA, which may be important for RNA replication and transcription.
Alternative Electrode Materials and Ceramic Filter Minimize Disinfection Byproducts in Point-of-Use Electrochemical Water Treatment (2013)

Effects of various electrodes and prefiltration to minimize disinfection byproducts (DBPs) in electrochemical water disinfection was evaluated. The target microorganism, <i>Escherichia coli</i> O157:H7, was effectively inactivated even applying a solar-charged storage battery for the electrolysis process. Extent of microbial inactivation decreased with lower water temperature and higher pH in the free chlorine disinfection system. The RuO<sub>2</sub>/Ti electrode was most efficient because it produced the lowest concentration of chlorate and the highest generation of free chlorine. Prefiltration using a ceramic filter inhibited formation of halogenated DBPs because it removed precursors of DBPs. For safe point-of-use water treatment, the use of a hybrid prefiltration stage with the electrolysis system is strongly recommended to reduce risks from DBPs. The system is particularly suited to use in developing regions.
A 155K Acid Carboxypeptidase O from<i>Aspergillus oryzae</i> (1986)

When Aspergillus oryzae IAM 2640 was grown in liquid medium, a high molecular weight acid CPase was produced exclusively in the medium. The enzyme was purified and characterized. The purified enzyme was homogeneous by PAGE at pH 9.4, by isoelectric focusing, and by FPLC, and its molecular weight was 155K by gel filtration. When the 155K enzyme was electrophoresed in SDS–polyacrylamide gel, a single band with a molecular weight of 73K was obtained. The enzyme was inhibited by TPCK and PMSF but not IAA or PCMB. The Km and kcat values of the enzyme for Z-Glu-Tyr, angiotensin, and bradykinin at pH 3.7 and 30°C were 0.48mm, 86sec-1, 0.1 mm, 0.34sec-1, and 0.06mm, 14.7sec-1, respectively. The enzyme hydrolyzed proangiotensin and bradykinin sequentially at pH 3.7 from their C-terminus. The enzyme shared an antigen with low molecular weight A. oryzae acid CPase O-1.
Multiple Disc Catalytic Converters - An Innovative Approach for Improved Conversion at Lower Cost (1994)

<div class="htmlview paragraph">The “Multiple Disc Converter” is an innovative concept. In comparison to conventional catalytic converters, it is significantly less expensive and more compact, at identical conversion efficiency and durability. The catalytic substrate consists of 4 to 5 cylindrical ceramic discs with 62 cells/cm2 (400 cells/inch<sup>2</sup>). These are assembled in a sheet metal casing, without gaps between the discs, and angularly offset to each other. The flow through the channels is interrupted at the contact surfaces of the individual discs. Hence, the restarted turbulent flow intensifies the transverse mass transfer. Further, the uniformity of the exhaust gas flow is improved, particularly in the first discs. Thus, the conversion of emissions is improved and the durability extended. When the conversion is maintained at the level of conventional converters, then the converter volume can be substantially reduced using the same specific catalyst loading. This is attained by a 10 to 15 % shortening of the total length. The economizing of precious metals, manufacturing savings from the more compact casing and the simplified canning system, all result in up to 10 % lower costs. The basic applicability for large scale production has been proved.</div>
Multi-focal video fusion with a beam splitter prism (2015)

This paper addresses the feasibility of using inexpensive, compact consumer-oriented cameras to resolve fundamental issues in capturing video footage of biological interactions in the field for quantitative analysis. The strengths and weaknesses of using a multi-camera beam splitter system to capture closely align video footage and its applications for studying biological interactions are discussed. The strength of the approach is that the closely aligned video streams are readily focal stacked to increase spatial information in a frame. Weaknesses include the limitation of the system in low light conditions where the beam splitter reduces the light captured by the cameras. Our novel system lends itself to incorporation into a camera array to capture large amounts of biological information.
Low back pain research priorities: a survey of primary care practitioners (2007)

Practitioners identified a need for more information on a variety of topics, including diagnosis, the effectiveness of treatments, and identification of patient characteristics which affect treatment and recovery.
A Cytochrome P450 Serves as an Unexpected Terpene Cyclase during Fungal Meroterpenoid Biosynthesis (2013)

Viridicatumtoxin (1) is a tetracycline-like fungal meroterpenoid with a unique, fused spirobicyclic ring system. Puzzlingly, no dedicated terpene cyclase is found in the gene cluster identified in Penicillium aethiopicum. Cytochrome P450 enzymes VrtE and VrtK in the vrt gene cluster were shown to catalyze C5-hydroxylation and spirobicyclic ring formation, respectively. Feeding acyclic previridicatumtoxin to Saccharomyces cerevisiae expressing VrtK confirmed that VrtK is the sole enzyme required for cyclizing the geranyl moiety. Thus, VrtK is the first example of a P450 that can catalyze terpene cyclization, most likely via initial oxidation of C17 to an allylic carbocation. Quantum chemical modeling revealed a possible new tertiary carbocation intermediate E that forms after allylic carbocation formation. Intermediate E can readily undergo concerted 1,2-alkyl shift/1,3-hydride shift, either spontaneously or further aided by VrtK, followed by C7 Friedel-Crafts alkylation to afford 1. The most likely stereochemical course of the reaction was proposed on the basis of the results of our computations.
Zona Pellucida Induces Activation of Phospholipase A2 During Acrosomal Exocytosis in Guinea Pig Spermatozoa1 (2003)

Phospholipase A(2) (PLA(2)) is activated in spermatozoa in response to progesterone and Ca(2+) ionophores, but to our knowledge, no study has yet reported zona pellucida (ZP)-induced activation of PLA(2). We investigated whether PLA(2) is involved in ZP-stimulated acrosomal exocytosis, if Ca(2+) is required for activation of PLA(2), and signal transduction pathways modulating PLA(2) using guinea pig sperm as a model. Spermatozoa were capacitated and labeled in low-Ca(2+) medium with [(14)C]choline chloride or [(14)C]arachidonic acid and were then exposed to millimolar Ca(2+) and various reagents and stimulated with ZP. Precapacitated spermatozoa exposed to millimolar Ca(2+) and stimulated with ZP experienced increases in arachidonic acid (AA) and lysophosphatidylcholine (lysoPC) levels and a parallel decrease in phosphatidylcholine level; these changes are indicative of PLA(2) activation. Simulation with ZP also led to acrosomal exocytosis in a high proportion of spermatozoa. Lipid changes and exocytosis were prevented if spermatozoa were exposed to aristolochic acid, a PLA(2) inhibitor, before treatment with ZP. Stimulation with ZP in medium without added Ca(2+) or in medium with millimolar Ca(2+) and EGTA or La(3+) resulted in no lipid changes or exocytosis. Pretreatment with pertussis toxin, a G(i) protein inhibitor, before stimulation with ZP blocked the release of AA and lysoPC as well as acrosomal exocytosis. Exposure of spermatozoa to the diacylglycerol (DAG) kinase inhibitor R59022 before ZP stimulation led to a significant increase in generation of lysoPC and exocytosis. Taken together, these results indicate very strongly that PLA(2) plays an essential role in ZP-induced exocytosis in spermatozoa, that PLA(2) activation requires Ca(2+) internalization, and that PLA(2) activation is regulated by signal transduction pathways involving G proteins and DAG.
Experimental study of charge-state distributions and photon emissions from beam-tilted-foil interactions (1978)

Relative spectral-line intensities of beam-foil-excited magnesium projectiles have been measured versus the projectile energy (100-900 keV) with carbon foils tilted 0\ifmmode^\circ\else\textdegree\fi{}, 30\ifmmode^\circ\else\textdegree\fi{}, and 45\ifmmode^\circ\else\textdegree\fi{}. The photon intensities depend upon the foil tilt angle. Charge-state distributions measured for ${\mathrm{H}}^{+}$, ${\mathrm{C}}^{+}$, ${\mathrm{N}}^{+}$, ${\mathrm{O}}^{+}$, ${\mathrm{Na}}^{+}$, ${\mathrm{Mg}}^{+}$, ${\mathrm{S}}^{+}$, and ${\mathrm{Kr}}^{+}$ transmitted through carbon foils tilted 0\ifmmode^\circ\else\textdegree\fi{}, 30\ifmmode^\circ\else\textdegree\fi{}, and 45\ifmmode^\circ\else\textdegree\fi{} (100-400 keV) were found not to be influenced by the tilt angle in contrast to the optical results. Charge-state distributions for C and S, which have not been reported earlier, are in agreement with a recently proposed independent-electron model for charge-state distributions and beam-foil populations.
Tapered Corrugated Waveguide Low-pass Filters (1973)

Several new synthesis techniques are described for the design of tapered corrugated waveguide low-pass filters. Previous techniques are based on image-parameter methods which are both nonoptimum and difficult to apply to new specifications. The new synthesis methods give filters which can be constructed to work directly from dimensions generated by a computer. The impedance tapering implies that the terminating impedance transformers used in the image-parameter designs may be either eliminated or reduced in length.
New technological vistas for systems and control: the example of wireless networks (2001)

Over the past four decades, the field of control has woven a rich tapestry of a larger systems theory, with sustained investigations into fundamental issues such as control, estimation, stability, optimality, adaptation, and decentralization. These issues are the fundamental ingredients in many new proposed technologies, which are now within our collective purview. They provide a profusion of practical examples of problems that may have sometimes abstractly engaged our attentions in the past and offer a wealth of opportunities for imaginative solutions. The opportunities are ours to seize. That is the central thesis of this article, and the author uses the example of wireless networking, an area of great interest in the emerging field of information technology, to illustrate it.
Statistical learning and multiple linear regression model for network selection using MIH (2014)

A key requirement to provide seamless mobility and guaranteeing Quality of Service in heterogeneous environment is to select the best destination network during handover. In this paper, we propose a new schema for network selection based on Multiple Linear Regression Model (MLRM). A thorough investigation, on a huge live data collected from GPRS/UMTS networks led to identify the Key Performance Indicators (KPIs) that play the most important role in the handover process. These KPIs are: Received Signal Code Power (RSCP), received energy per chip (Ec/No)and Available Bandwidth (ABW) of the destination network. To extract a handover model from collected data, we study the correlation among values of identified KPIs parameters, before, during and after handover, thanks to a statistical learning approach, using the predictive analytics software SPSS. For model assessment, Pearson Correlation Coefficient and determination coefficient R-squared (R <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> ) are used. Media Independent Handover (MIH) IEEE 802.21 standard is used in this work to retrieve the lower layer information of available networks and announce the handover needs (handover initiation). The proposed model will help to select the most appropriate network between many existing ones in the vicinity of the mobile node.
Pigmentation and color comparison of Ruby Red and Star Ruby grapefruit juice (1979)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPigmentation and color comparison of Ruby Red and Star Ruby grapefruit juiceRobert R. Cruse, Bruce J. Lime, and Richard A. HenszCite this: J. Agric. Food Chem. 1979, 27, 3, 641–642Publication Date (Print):May 1, 1979Publication History Published online1 May 2002Published inissue 1 May 1979https://pubs.acs.org/doi/10.1021/jf60223a029https://doi.org/10.1021/jf60223a029research-articleACS PublicationsRequest reuse permissionsArticle Views98Altmetric-Citations15LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Grounded auditory development by a developmental robot (2002)

A developmental robot is one that learns and practices autonomously in the real physical world by interacting with the environment through sensors and effecters, probably under human supervision. The study of developmental robots is motivated by the autonomous developmental process of higher animals and humans from infancy to adulthood. Our goal is to enable a robot to learn autonomously from real-world experiences. The paper presents a case study of a developmental robot developing its auditory related behaviors to follow human trainers' voice commands. A learning architecture is proposed to resolve automatic representation generation and selective attention issues. Both simulation results and experiments on a real robot are reported to show the effectiveness.
Silicon solar cell development for concentrated-sunlight, high-temperature applications (1976)

The development of silicon solar cells for use in concentrated-sunlight, high-temperature environments, based on theory and experiment, is presented. The theoretical work is aided by exact numerical simulations of the cells. Suggested cell designs are then fabricated using standard integrated circuit processing techniques. The designs are verified through computer-controlled testing of the cells at solar illuminations up to 90 suns (9 W/cm/sup 2/) and temperatures up to 100/sup 0/C. The development of a 5-cm-diameter silicon wafer cell for a 50-sun, approximately 100/sup 0/C application is emphasized.
The multiphoton ionization spectrum of <i>t</i> <i>r</i> <i>a</i> <i>n</i> <i>s</i>-1,3-butadiene (1976)

The multiphoton ionization spectrum of trans-1,3-butadiene has been measured from dye laser wavelengths of 365 to 468 nm using an intensity-corrected system. In the four-photon ionization region to lower energy than 405 nm, many three-photon resonances with Rydberg states are seen and a new series with a quantum defect of 0.04 is identified. The symmetries of the observed Rydberg series are discussed. Above 405 nm in energy, a resonance occurs with the ? state seen in one-photon absorption spectra, only the structure in the multiphoton ionization spectrum is characteristic of an allowed two-photon resonance. From the vibronic structure of this state, it is argued that it has symmetry 1Bg. In the region covered by this study (42 700 to 54 800 cm−1 in two-photon energy) no state was found to which a 1Ag symmetry designation could be applied.