gebhart/patrepeval huggingface dataset:
Use of oligonucletides to define the site of interstand cross-links induced by Adriamycin (1995)

It has been known for several years that Adriamycin forms adducts and interstrand cross-links when reacted for long periods of time with bacterial and mammalian DNA in vitro, with the cross-link being restricted to 2 bp elements containing GpC sequences. The self-complementary 20mer deoxyoligonucleotide TA4T4GCA4T4A has been used in this study as a model of the apparent G-G cross-linking site at GpC sequences. The rate of formation of cross-links, as well as the dependence on both Adriamycin and Fe(III) concentration, were similar with this oligonucleotide as compared with calf thymus DNA. The cross-linking was demonstrated on both denaturing and non-denaturing sequencing gels. The half-life of the G-G cross-link was 40 h, consistent with that implied with high molecular weight, heterogeneous sequence DNA. Exonuclease III digests of adducts formed with 20mer deoxyoligonucleotides containing single, central G-G, G-I and I-I potential cross-links revealed that a guanine residue is required at both ends of the cross-link. No cross-linking was observed with a similar oligonucleotide containing only a single central (G.C) bp.
Application of a mass storage system to science data management (2002)

Approaches to the issues of data ingestion, data restructuring, physical data models, relationship file structure to data system performance, data product generation, data transfer to remote users, data subset extraction, data browsing, and user interface have been examined. The High Performance Data System architecture provides an environment for bringing together the technologies of mass storage, large bandwidth data networks, high-performance data processing, and intelligent data access. The prototype system demonstrates an approach to these issues. In addition, the design process has defined some important requirements for the mass storage file system, such as logical grouping of files, aggregate file writes, and multiple dynamic storage device hierarchies.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
TENS (transcutaneous electrical nerve stimulation) for labour pain. (2012)

Because TENS is applied inconsistently and not always in line with optimal TENS application theory, this may explain why TENS for labour pain appears to be effective in some individuals and not in others. This article reviews TENS theory, advises upon optimal TENS application for labour pain and discusses some of the limitations of TENS research on labour pain. TENS application for labour pain may include TENS applied to either side of the lower spine, set to 200 mus pulse duration and 100 pulses per second. As pain increases, TENS intensity should be increased and as pain decreases, TENS intensity should be reduced to maintain a strong but pain free intensity of stimulation. This application may particularly reduce back pain during labour.
Robust Classification of Abnormal Audio using Background-Foreground Separation (2017)

Surveillance systems are largely based on visual mode, but acoustic mode of surveillance have gained importance in the recent times. Detection of abnormal sounds can greatly contribute for surveillance purposes. Audio signal processing has always been challenging due to dynamically changing background noise. Audio event recognition rates are to be increased and false positive rates are to be reduced to build more efficient systems. The proposed methodology focuses on reducing false alarm rates by implementing a Background-Foreground Separation module. Separation of foreground events from the background audio is achieved by introducing an Impulsive Sound Detection module. In the classification stage, k-Nearest Neighbor classifier with Mel Frequency Cepstral Coefficients (MFCC) features are employed. The method is validated on a large publicly available dataset which is developed for acoustic event classification. A recognition rate of 94.07% and a low false alarm rate of 1.23% is achieved which is significant improvement compared to the state-of-art approaches.
Parameter Identification of a Numerical Transport Code (2002)

A parameter identification process is presented to obtain effective, resultant properties of a numerical transport code. The method is demonstrated using NUFT (Nonisothermal Unsaturated-Saturated Flow and Transportation model) [1], as a transport code, and the effective heat conductivity and thermal diffusivity as effective parameters. A numerical application example shows that effective transport code in a variety of boundary conditions.
BAG3 gene silencing sensitizes leukemic cells to Bortezomib‐induced apoptosis (2008)

Proteasome inhibition has emerged as a powerful option for the treatment of a number of malignancies including leukemias. However, Bortezomib showed limited single-agent activity for patients with leukemia. Here, we report for the first time that Bortezomib up-regulated a novel antiapoptotic protein, BAG3, in human leukemic cells. BAG3 gene knockdown with shRNA greatly potentiated the generation of apoptosis by Bortezomib in leukemia cells. Furthermore, BAG3 silencing enhanced the antitumor activity of Bortezomib dramatically in a nude mouse model. Our results indicate that knocking down BAG3 gene is a promising new approach to enhance the therapeutic potency of Bortezomib in leukemia.
Demonstration of Channel Multiplexing Quantum Communication Exploiting Entangled Sideband Modes (2020)

Channel multiplexing quantum communication based on exploiting continuous-variable entanglement of optical modes offers great potential to enhance channel capacity and save quantum resource. Here, we present a frequency-comb-type control scheme for simultaneously extracting a lot of entangled sideband modes with arbitrary frequency detuning from a squeezed state of light. We experimentally demonstrate fourfold channel multiplexing quantum dense coding communication by exploiting the extracted four pairs of entangled sideband modes. Due to high entanglement and wide frequency separation between each entangled pairs, these quantum channels have large channel capacity and the cross talking effect can be avoided. The achieved channel capacities have surpassed that of all classical and quantum communication under the same bandwidth published so far. The presented scheme can be extended to more channels if more entangled sideband modes are extracted.
Reaction of conjugated enones with divinylcopperlithium tri-<i>n</i>-butylphosphine complex. A new preparation of γ,δ-unsaturated ketones (1970)

A new synthesis of γ,δ-unsaturated ketones is reported, based on the reaction of α,β-unsaturated ketones with a vinyl ate complex.
Selective Access to 3-Cyano-1<i>H</i>-indoles, 9<i>H</i>-Pyrimido[4,5-<i>b</i>]indoles, or 9<i>H</i>-Pyrido[2,3-<i>b</i>]indoles through Copper-Catalyzed One-Pot Multicomponent Cascade Reactions (2015)

Novel and selective synthetic approaches toward indole derivatives via copper-catalyzed one-pot multicomponent cascade reactions of 1-bromo-2-(2,2-dibromovinyl)benzenes with aldehydes and aqueous ammonia are presented. Intriguingly, the concentration of ammonia, the molar ratio of reagents, and the structural features of the aldehyde substrate serve as key factors in controlling the selective formation of 3-cyano-1H-indoles, 9H-pyrimido[4,5-b]indoles, or 9H-pyrido[2,3-b]indoles. Compared with literature procedures, the synthetic approaches reported herein have advantages such as readily available starting materials, mild reaction conditions, and divergent reaction patterns toward different products with easily tunable selectivity.
Using input condition monitoring to improve the stability of digitally controlled power factor correcting converters (2010)

An adaptive controller for a PFC converter is proposed and demonstrated. This controller is applied to a converter with a boost topology operating in continuous conduction mode with average current control. The compensator adjusts a variable gain in the current loop so as to counteract the effects of the time-varying source impedance and input voltage. Experimental results verify that the proposed controller improves the stability of the interaction between the converter and a non-ideal source.
Efflorescence Resulting from Pyrite in Clay Raw Materials (1958)

In opening this investigation on the effect of pyrite on the efflorescence of structural clay products, the oxidation behavior of this sulfide was studied. The slow oxidation rate and the evolution of 2 moles of sulfur dioxide for every mole of pyrite present combined to make this impurity the major source of efflorescing salts. The retention of part of the sulfur dioxide by the clay material and the subsequent efflorescence tendency were found to depend on the composition, on the concentration of sulfurous gases, on the temperature of firing, on the time of firing, and on the duration of exposure to sulfur dioxide in the manufacturing process. The conditions necessary to reduce efflorescence from this source are described. The use of ammonium chloride to assist in the control of efflorescence was studied in detail.
Renal gene expression. (1993)

The advent of cloned DNA probes has revolutionized the study of cells and tissues via the technique of hybridization histochemistry. Because of the intrinsic specificity of the probes the application of the technique to functionally complex and morphologically diverse tissue like the kidney has been especially rewarding. The technology has been advanced enormously by the parallel development of synthesized DNA or oligonucleotides. A specific synthetic probe can be manufactured in a day, purified, labeled and used to determine in which cells a particular gene of interest is being expressed ("switched on"). The applications of this technology are particularly opposite in the renal field. They can provide unique insights into normal physiological processes and will provide new diagnostic approaches of unparalleled specificity. Some specific examples have been chosen over a wide range of institute programs to highlight the potential value of the technique of hybridization histochemistry and to emphasize its potential for studying renal physiology and pathology.
Chemical Equilibria of Molten Ternary Nitrate Salt Mixtures (1989)

We have evaluated the chemical equilibria of the decomposition reactions of ternary mixtures of molten nitrate salts consisting of sodium and potassium nitrate with additions of either calcium, barium or lithium nitrate. We found that the equilibrium constant of the initial reaction, nitrate nitrite + oxygen, is relatively insensitive to the composition of the mixtures. The extent to which subsequent decomposition reactions proceed is dependent on the type and amount of the additions to the binary alkali nitrate mixture and is related to the charge and size of the added cationic species. The interaction of atmospheric carbon dioxide with the melts was evaluated for alkaline earth-containing mixtures.
Responsiveness of two urea cycle enzymes in Morris hepatomas to metabolic modulations. (1971)

Arginase and argininosuccinate synthetase activities have been assayed in some 14 hepatomas and the corresponding host livers. The hepatomas exhibited an extremely wide range of activities of the two enzymes of the urea cycle. A good correlation exists between the activities of the two enzymes in the hepatomas. However, the activity of either enzyme in the hepatomas bears no correlation with that in the host livers. Moreover, there is no correlation between the enzyme activity and the growth rate or chromosome number of the hepatomas.

Cortisol raised arginase activity to a larger degree in Hepatomas 7800 and 8999 than in the host livers, but the hormone enhanced argininosuccinate synthetase activity more in the host livers than in the two hepatomas. Thyroxine increased arginase activity in Hepatoma 7800 and the host liver of intact rats but decreased it in adrenalectomized rats. The thyroid hormone also elevated the synthetase activity in Hepatoma 7800 but not in the host liver. On the other hand, thyroxine did not affect the activity of these two enzymes in either Hepatoma 9618A or the host liver.

While actinomycin D or cycloheximide blocked the hormone-induced increase in arginase activity of Hepatomas 7800 and 8999 and in the synthetase activity of Hepatoma 7800, the antibiotics exerted no inhibitory effect on the enzymes in the host livers. Increasing the protein content of the diet elicited increases in the activity of the two enzymes in Hepatoma 9618A, as well as in the host liver, but not in Hepatoma 8999.

These results together with others show an overresponse by enzymes in hepatomas, when compared with the response by the same enzymes in host livers. This overresponsiveness is discussed as a manifestation of deranged control mechanisms in cancer.
An Analysis of Uncertainty in Directional Surveying (1969)

Knowledge concerning the position of the bottom of a directionally drilled hole is uncertain. This uncertainty can be measured quantitatively either by analysis based on principles of probability and statistics, or by Monte Carlo simulation using a computer that yields a pattern of possible bottomhole locations. Introduction and Model Description In view of the large number of wells directionally drilled from offshore platforms and urban drillsites, greater interest is being focused on the directional survey and on the uncertainty inherent in calculating the position of the bottom of the hole, or other benchmarks in the wellbore. Of the two methods presented here for evaluating this uncertainty, the first is analytical and uses the principles of probability and statistics. The second method uses Monte Carlo simulation to generate a pattern of the scatter of possible bottom-hole locations. possible bottom-hole locations. Both methods are based on a model for which the usual assumption is made that the curvilinear axis of the wellbore is adequately represented by a large number, M, of linear segments joined end to end from the top to the bottom of the hole and closely following the course of the hole. The errors due to this approximation are assumed to be negligible and are not studied here. One form of the model is to consider that for a given hole there exists a perfect set of data describing it, of the above form. Each linear segment has an angle of inclination with the vertical Ii in radians, an azimuthal angle from true north Ai in radians, and a length Si, in feet. The subscript variable, i, extends from 1 to M. Each of the M linear segments representing the axis of the wellbore has three components in the X, Y, and Z directions. These components are calculated from the equations:....................(1) The bottom-hole coordinates as determined from the reference set of data for the wellbore are given by the following for i = 1 to M:..........(2) A directional survey instrument, lowered into the wellbore to the M segments, or stations, measures the values of the Ii and the Ti. In general, because of errors from various sources, the resulting angular measurements will not be precisely equal to the I, and Ai. Random variables Ii precisely equal to the I, and Ai. Random variables Ii and Ai are therefore considered with associated probability density functions fi(Ii) and gi(Ai), probability density functions fi(Ii) and gi(Ai), respectively, which represent the distributions of potential survey measurements for this hole. Since it is assumed that the values Si are accurately measured they are treated deterministically. The random variables corresponding to the surveyed bottom-hole coordinates are given, for i = 1 to M, by:............(3) P. 515
Sensitivity-Based Approaches for Handling Discrete Variables in Optimal Power Flow Computations (2010)

This paper proposes and compares three iterative approaches for handling discrete variables in optimal power flow (OPF) computations. The first two approaches rely on the sensitivities of the objective and inequality constraints with respect to discrete variables. They set the discrete variables values either by solving a mixed-integer linear programming (MILP) problem or by using a simple procedure based on a merit function. The third approach relies on the use of Lagrange multipliers corresponding to the discrete variables bound constraints at the OPF solution. The classical round-off technique and a progressive round-off approach have been also used as a basis of comparison. We provide extensive numerical results with these approaches on four test systems with up to 1203 buses, and for two OPF problems: loss minimization and generation cost minimization, respectively. These results show that the sensitivity-based approach combined with the merit function clearly outperforms the other approaches in terms of: objective function quality, reliability, and computational times. Furthermore, the objective value obtained with this approach has been very close to that provided by the continuous relaxation OPF. This approach constitutes therefore a viable alternative to other methods dealing with discrete variables in an OPF.
Novel MOS resistive circuit for synthesis of fully integrated continuous-time filters (1986)

This paper describes a new MOS resistive circuit containing four matched MOS transistors. It allows to fully integrate known active RC filter structures on a single chip without externals components. Integrator, summer and lossy integrator circuits with a single operational amplifier are shown. The new circuits improve on previously suggested MOS active filters.
On the structure and control of commands (1973)

An interactive command language, with its underlying data, defines a command environment. In general a command environment supports a number of commands which once issued perform non-interactively, and which when finished leave the old command environment in control. It also supports some special commands which move to other command environments, after which commands are interpreted according to a different set of rules.
NON-SPOREFORMING BACTERIA PATHOGENIC TO INSECTS: INCIDENCE AND MECHANISMS (1985)

Microbiology began as a unified science using the principles of chemistry to understand living systems. The unified view quickly split into the subdisciplines of medical microbiology, molecular biology, and environmental microbiology. The advent of a ...Read More
Effect of zein on biodegradable inserts for the delivery of tetracycline within periodontal pockets (2011)

Treatment with antibiotics within the periodontal pocket against bacterial infections represents a useful and adjunctive tool to conventional therapy for healing and teeth preservation. With this function in view, an implantable, tetracycline delivery device for the treatment of periodontal disease was developed. The aim of this study was to develop biodegradable, tetracycline-loaded microparticles made of two polymers: PLGA and zein which were compressed into monolithic devices. In this polymer delivery system, the encapsulation efficiency, release characteristics, drug-polymer interaction, and antibacterial activity of loaded drug were investigated. The interaction of tetracycline with the corn protein zein was studied by nuclear magnetic resonance (NMR), Fourier transform infrared, and X-ray diffraction. The hydrophobic interaction of tetracycline with zein in the formulations was deduced from the NMR studies, whereas X-ray diffraction studies showed a new crystalline state of the drug in the presence of the protein. Zein was not denatured by preparation of inserts. Sustained release of tetracycline was obtained, and the proportion of zein in the inserts had a great impact on the drug release. Finally, an effective tetracycline release from inserts against Staphylococcus aureus was achieved over 30 days. In conclusion, the PLGA:zein delivery system described in this study was found to be effective in controlled delivery of tetracycline, and hence may be suitable for intra-pocket delivery of antimicrobial agents in the treatment of periodontitis.
The Central Governor Model in 2012: eight new papers deepen our understanding of the regulation of human exercise performance (2011)

<h3>Abstract</h3> <h3>Motivation</h3> With an increasing number of patient-derived xenograft (PDX) models being created and subsequently sequenced to study tumor heterogeneity and to guide therapy decisions, there is a similarly increasing need for methods to separate reads originating from the graft (human) tumor and reads originating from the host species’ (mouse) surrounding tissue. Two kinds of methods are in use: On the one hand, alignment-based tools require that reads are mapped and aligned (by an external mapper/aligner) to the host and graft genomes separately first; the tool itself then processes the resulting alignments and quality metrics (typically BAM files) to assign each read or read pair. On the other hand, alignment-free tools work directly on the raw read data (typically FASTQ files). Recent studies compare different approaches and tools, with varying results. <h3>Results</h3> We show that alignment-free methods for xenograft sorting are superior concerning CPU time usage and equivalent in accuracy. We improve upon the state of the art sorting by presenting a fast lightweight approach based on three-way bucketed quotiented Cuckoo hashing. Our hash table requires memory comparable to an FM index typically used for read alignment and less than other alignment-free approaches. It allows extremely fast lookups and uses less CPU time than other alignment-free methods and alignment-based methods at similar accuracy. <h3>Availability</h3> Our software <i>xengsort</i> is available under the MIT license at http://gitlab.com/genomeinformatics/xengsort. It is written in numba-compiled Python and comes with Snakemake workflows for hash table construction and dataset processing. <h3>Contact</h3> Sven.Rahmann@uni-due.de
G protein‐coupled receptor 84, a microglia‐associated protein expressed in neuroinflammatory conditions (2007)

G protein-coupled receptor 84 (GPR84) is a recently discovered member of the seven transmembrane receptor superfamily whose function and regulation are unknown. Here, we report that in mice suffering from endotoxemia, microglia express GPR84 in a strong and sustained manner. This property is shared by subpopulations of peripheral macrophages and, to a much lesser extent, monocytes. The induction of GPR84 expression by endotoxin is mediated, at least in part, by proinflammatory cytokines, notably tumor necrosis factor (TNF) and interleukin-1 (IL-1), because mice lacking either one or both of these molecules have fewer GPR84-expressing cells in their cerebral cortex than wild-type mice during the early phase of endotoxemia. Moreover, when injected intracerebrally or added to microglial cultures, recombinant TNF stimulates GPR84 expression through a dexamethasone-insensitive mechanism. Finally, we show that microglia produce GPR84 not only during endotoxemia, but also during experimental autoimmune encephalomyelitis (EAE), a model of multiple sclerosis. In conclusion, this study reports the identification of a new sensitive marker of microglial activation, which may play an important regulatory role in neuroimmunological processes, acting downstream to the effects of proinflammatory mediators.
Dendritic tellurides acting as antioxidants (2006)

我们描述了合成一系列(芳基醚) poly，在核心和 oligo (环氧乙烷) 的 dendrimerswith 碲化物在作为谷胱甘肽，过氧物(GPx ) 模仿行为的圆周锁住。这些混合物的系列被 ~ 1H-NMR， ~(13 ) C-NMR 和 ESIMS 很好描绘。用不同 ROOH (H_2O_2，茴香素水疗院过氧化物) 因为测试这些的 theantioxidizing 性质加重，我们发现了从产生 0 ～ 2，树枝状的 GPx 的活动模仿第一减少然后增加。这能根据更大的位阻现象被解释，从产生 0 ～ 1 去，并且更强壮的有约束力的相互作用从产生 1 ～ 2 去。换句话说，在那里存在在可以优化 GPx 活动的有约束力的相互作用和位阻现象之间的平衡。