gebhart/patrepeval huggingface dataset:
Combined Effect of Improved Cell Yield and Increased Specific Productivity Enhances Recombinant Enzyme Production in Genome-Reduced Bacillus subtilis Strain MGB874 (2011)

Genome reduction strategies to create genetically improved cellular biosynthesis machineries for proteins and other products have been pursued by use of a wide range of bacteria. We reported previously that the novel Bacillus subtilis strain MGB874, which was derived from strain 168 and has a total genomic deletion of 874 kb (20.7%), exhibits enhanced production of recombinant enzymes. However, it was not clear how the genomic reduction resulted in elevated enzyme production. Here we report that deletion of the rocDEF-rocR region, which is involved in arginine degradation, contributes to enhanced enzyme production in strain MGB874. Deletion of the rocDEF-rocR region caused drastic changes in glutamate metabolism, leading to improved cell yields with maintenance of enzyme productivity. Notably, the specific enzyme productivity was higher in the reduced-genome strain, with or without the rocDEF-rocR region, than in wild-type strain 168. The high specific productivity in strain MGB874 is likely attributable to the higher expression levels of the target gene resulting from an increased promoter activity and plasmid copy number. Thus, the combined effects of the improved cell yield by deletion of the rocDEF-rocR region and the increased specific productivity by deletion of another gene(s) or the genomic reduction itself enhanced the production of recombinant enzymes in MGB874. Our findings represent a good starting point for the further improvement of B. subtilis reduced-genome strains as cell factories for the production of heterologous enzymes.
An 802.11k Compliant Framework for Cooperative Handoff in Wireless Networks (2009)

In IEEE 802.11-based wireless networks, the stations (STAs) are associated with the available access points (APs) and communicate through them. In traditional handoff schemes, the STAs get information about the active APs in their neighborhood by scanning the available channels and listening to transmitted beacons. This paper proposes an 802.11k compliant framework for cooperative handoff where the STAs are informed about the active APs by exchanging information with neighboring STAs. Besides, the APs share useful information that can be used by the STAs in a handoff process. In this way, we minimize the delay of the scanning procedure. We evaluate the performance of our mechanisms through OPNET simulations. We demonstrate that our scheme reduces the scanning delay up to 92%. Consequently, our system is more capable in meeting the needs of QoS-sensitive applications.
Human hypertensive placenta contains an increased amount of Na, K‐ATPase with higher affinity for cardiac glycosides (1994)

Placentas of women suffering from pregnancy-induced hypertension (PIH) were found to contain a greater amount of Na,K-ATPase molecules, estimated from anthroyl ouabain binding, than normotensive individuals. Both the microsomal fraction of placental cells and purified Na,K-ATPase showed an increased affinity for the specific inhibitor ouabain which, in the case of the microsomes, bound with a dissociation constant of 0.9 nM as compared with 3.4 nM in the controls. Likewise, the dissociation constant of the ouabain complex with purified Na,K-ATPase was about 3.5 times lower in the hypertensive patients. The differences are apparently caused by a different microenvironment of the ouabain-binding site, as reflected in the quantum yield of bound anthroyl ouabain. If an endogenous digitalis-like factor is present in the body fluids to regulate Na,K-ATPase activity, the present results render its role quite plausible.
Modulation of P-selectin expression on isolated human platelets assessed by a novel ELISA application (1996)

Modulation of P-selectin expression on isolated human platelets assessed by a novel ELISA application
The past, present and future of audio signal processing (1997)

The article reviews, with technical depth, the history of research in audio and electroacoustics, including electroacoustic devices, noise control, echo cancellation, and psychoacoustics. It also reports on processes in signal processing technologies that are widely used in hearing aids, the consumer electronics area, and professional audio equipment. It provides an effective review of the history of signal processing.
Characterizing Adult Cervical Deformity and Disability Based on Existing Cervical and Adult Deformity Classification Schemes at Presentation and Following Correction (2017)

Cervical deformities displayed higher TS-CL grades and different cSVA grade distributions. Preoperative associations with global alignment modifiers and Ames-ACD descriptors were observed, though only cervical modifiers showed postoperative differences.
A Numerical Investigation of the Detachment of the Trailing Particle From a Chain Sedimenting in Newtonian and Viscoelastic Fluids (2000)

Particles sedimenting in a viscoelastic fluid form chains. It has been observed in experiments that, sometimes, the last particle in the chain gets detached. In this paper, we investigate this phenomenon. It is known that a long chain falls faster than a single particle in fluids. This long body effect tends to detach the last particle from the chain. The wake effect and the normal stress effect are the mechanisms that work against the long body effect. The last particle is not detached if the inertial wake effects are strong enough to cause substantial drag reduction on it. The detachment is also restricted by the elastic normal stress of the viscoelastic fluid. [S0098-2202(00)01003-8]
Abstract 16576: Intravenous Infusion of Cardiosphere-Derived Cells and their Exosomes Improve Dystrophin-Deficient Cardiomyopathy in mdx Mice (2017)

Duchenne muscular dystrophy (DMD) is a lethal muscle wasting disease caused by genetic lesions in the Dmd gene. Patients not only experience skeletal muscle weakness and pulmonary insufficiency, they also develop severe cardiomyopathy - one of the main causes of premature death. Secondary to the loss of dystrophin, the heart exhibits chronic inflammation, oxidant stress, fibrosis, and cardiomyocyte loss, which directly contribute to the pathogenesis of the disease. There is no cure for DMD, and nothing has been proven to slow the progression of cardiomyopathy. Cardiosphere-derived cells (CDCs) are cardiac progenitor cells with anti-inflammatory, anti-oxidant, anti-fibrotic, and regenerative properties, making them a logical therapeutic candidate. Previous work has demonstrated that CDCs are efficacious in treating dystrophic cardiomyopathy in mdx mice when injected directly into the myocardium, which motivated the ongoing HOPE-Duchenne clinical trial. Here, we sought to explore the efficacy of CDCs to tre...
Oligonucleotides Site-specifically Spin-labeled at 5'-Terminal or Internucleotide Linkage and Their Use in Gene Analyses (1993)

Spin-labeled oligonucleotides (S-probes) were synthesized and examined as DNA probes to monitor hybrid formation. TEMPO was introduced either at the internucleotide linkage of 5'-terminus (Type 1) or at the 5'-terminal hydroxyl group (Type 2) and both types of S-probes were used in this study. The presence of target DNA was detected in solution by EPR spectroscopy for both types of S-probes. Hybridization of the S-probes resulted in notable broadening of EPR line width, accompanied by a decrease in the EPR signal height ratio for I(-1)/I(0).I(-1)/I(0) of S-probes having no spacer between oligonucleotide and TEMPO decreased more markedly than that of S-probes with a spacer, indicating that TEMPO should be introduced to an oligonucleotide directly to monitor hybrid formation. When M13mp8 single-stranded DNA with or without an EcoRI recognition site was selected as a target DNA, hybrid formation was detected only for DNA containing EcoRI site in solution using spin-labeled oligonucleotides.
The pure rotational spectrum and hyperfine structure of CF studied by laser magnetic resonance (1982)

Laser magnetic resonance spectra have been measured for four rotational transitions and one spin-changing transition in the 2Π ground state of CF, generated in an intracavity methane–fluorine flame. From a detailed analysis of the Zeeman hyperfine structure of the J = 9/2→11/2 transition in the Ω = 3/2 spin component the hyperfine constants h, b, and d as well as B0 and q0 have been determined. Using these fitted parameters in conjunction with ab initio results, the values of 〈1/r3〉, 〈(3 cos2ϑ−1)/r3〉, ‖ψ2(0)‖, and 〈(sin2ϑ)/r3〉, averaged over the unpaired electron distribution, have been determined. Comparison of these integrals with those of the fluorine atom indicates that the unpaired electron has approximately 18% F character, implying a substantial degree of double bonding.
Identification of broad-based HIV-1 protease inhibitors from combinatorial libraries (2010)

Clinically approved inhibitors of the HIV-1 protease function via a competitive mechanism. A particular vulnerability of competitive inhibitors is their sensitivity to increases in substrate concentration, as may occur during virion assembly, budding and processing into a mature infectious viral particle. Advances in chemical synthesis have led to the development of new high-diversity chemical libraries using rapid in-solution syntheses. These libraries have been shown previously to be effective at disrupting protein-protein and protein-nucleic acid interfaces. We have screened 44000 compounds from such a library to identify inhibitors of the HIV-1 protease. One compound was identified that inhibits wild-type protease, as well as a drug-resistant protease with six mutations. Moreover, analysis of this compound suggests an allosteric non-competitive mechanism of inhibition and may represent a starting point for an additional strategy for anti-retroviral therapy.
A Slower Speed of Light: Developing intuition about special relativity with games. (2013)

The effects of special relativity are not immediately observable in our everyday lives, so we tend to lack an intuition about them. For this reason, special relativity is often considered too abstract a concept for introductory physics courses. Games may provide one solution to this problem. In this paper, we introduce A Slower Speed of Light, a game by the MIT Game Lab to help students understand and visualize these complex phenomena by artificially lowering the speed of light to walking pace. We also discuss future directions for the game, as well as the release of OpenRelativity, an open-source relativistic toolkit for Unity3D.
Aerobic Ruthenium‐Catalyzed Oxidative Transformation of Secondary Amines to Imines. (2007)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Breakthrough invasive pulmonary aspergillosis despite empirical voriconazole therapy for febrile neutropenia: Case report and review of the literature (2007)

We report the development of invasive pulmonary aspergillosis in a patient treated for acute myeloid leukaemia during empirical voriconazole therapy for febrile neutropenia. The patient failed to respond to the institution of salvage combination therapy with amphotericin B and voriconazole, but survived after adjunctive surgical resection.
21. U (2002)

PreviousNext No AccessEncyclopedic Dictionary of Applied Geophysics, Fourth edition21. UAuthors: Robert E. SheriffRobert E. SheriffProfessor, University of Houstonhttps://doi.org/10.1190/1.9781560802969.chu SectionsAboutPDF/ePub ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InRedditEmail Permalink: https://doi.org/10.1190/1.9781560802969.chuFiguresReferencesRelatedDetails Encyclopedic Dictionary of Applied Geophysics, Fourth editionISBN (print):978-1-56080-118-4ISBN (online):978-1-56080-296-9Copyright: 2002 Pages: 442 publication data© 2002 All rights reserved. No part of this publication may be reproduced or distributed in any form or by any means without written permission of the publisherPublisher:Society of Exploration Geophysicists HistoryPublished in print: 01 Jan 2002 CITATION INFORMATION Robert E. Sheriff, (2002), "21. U," Geophysical References Series : 372-374. https://doi.org/10.1190/1.9781560802969.chu Plain-Language Summary PDF DownloadLoading ...
Vliv tvorby hodnoty pro zákazníka na konkurenceschopnost podniku (2008)

Diplomovou praci na tema „Vliv tvorby hodnoty pro zakaznika na konkurenceschopnost podniku“ jsem zaměřil na využiti potencionalnich zdrojů konkurencni výhody eliminaci slabých stranek s využitim procesniho řizeni v konkretnim podniku. Hlavnimi ukoly bylo zlepsit komunikaci cinnosti a navrhnout decentralizaci v jedne dilci oblasti. Soucasti prace take bylo vytvořit komplexni zasady hodnotoveho managementu a zhodnotit kvalitu řizeni procesů. Prace byla zaměřena na zvysovani hodnoty pro zakaznika, ktera byla hlavnim ekonomickým kriteriem, jehož prostřednictvim jsem zvysoval konkurenceschopnost, respektive efektivnost a výkonnost podniku.
Depressive Symptom Clusters in Relation to Body Weight Status: Results From Two Large European Multicenter Studies (2019)

<b>Background:</b> There is strong evidence for a bidirectional association between depression and obesity. Several biological, psychological, and behavior-related factors may influence this complex association. Clinical impression and preliminary evidence suggest that patients with a diagnosis of major depressive disorder may endorse very different depressive symptom patterns depending on their body weight status. Until now, little is known about potential differences in depressive symptoms in relation to body weight status. <b>Objective:</b> The aim of this analysis is the investigation of potential differences in depressive symptom clusters (mood symptoms, somatic/vegetative symptoms, and cognitive symptoms) in relation to body weight status. <b>Methods</b>: Cross-sectional baseline data were derived from two large European multicenter studies: the MooDFOOD Trial and the NESDA cohort study, including persons with overweight and obesity and normal weight reporting subthreshold depressive symptoms (assessment <i>via</i> Inventory of Depressive Symptomatology Self-Report, IDS-SR30). Different measures for body weight status [waist-to-hip ratio (WHR) and body mass index (BMI)] were examined. Propensity score matching was performed and multiple linear regression analyses were conducted. <b>Results</b>: A total of <i>n</i> = 504 individuals (73.0% women) were analyzed. Results show that more somatic/vegetative depressive symptoms, such as pain, change in appetite and weight, gastrointestinal symptoms, and arousal-related symptoms, were significantly associated with both a higher BMI and higher WHR, respectively. In addition, being male and older age were significantly associated with higher WHR. Mood and cognitive depressive symptoms did not yield significant associations for both body weight status measures. <b>Conclusions:</b> Somatic/vegetative symptoms and not mood and cognitive symptoms of depression are associated with body weight status. Thus, the results support previous findings of heterogeneous depressive symptoms in relation to body weight status. In addition to BMI, other body weight status measures for obesity should be taken into account in future studies. <b>Clinical Trial Registration:</b> www.ClinicalTrials.gov, identifier NCT02529423.
A scalable analog architecture for neural networks with on-chip learning and refreshing (2002)

This paper discusses various techniques for analog storage and handling and proposes a new class of architecture suitable for modular and scalable analog neural networks with on-chip learning and refreshing. The new architecture is based on analog functional blocks and analog pass switches which enhance the system versatility. Supporting algorithms are also developed. A novel characteristic is the full-analog on-chip learning methodology which substantially increases the learning speed. The speedup is evidenced by the utilization of local analog synaptic updating scheme which utterly eliminates time-sharing components. Moreover, this localization scheme conceives unbounded scalability in this neural architecture.
Overload and Ultimate Load Behavior of Posttensioned Slab Bridge (2000)

A three-tenths-scale model of a posttensioned slab bridge is constructed in a laboratory and tested in the overload and ultimate load ranges. Dimensions of the slab are 16.9 × 5.33 × 0.23 m. In addition to uniformly distributed longitudinal posttensioning, a band of tendons is located in a narrow region directly above the supporting columns. A large array of strain gauges, linear variable differential transformers, and load cells serve to gather data for each loading configuration. Results are presented for a series of overloads that are placed on the slab in a variety of critical locations. Ultimate positive bending moment is imposed on one span and an ultimate negative moment is applied to the slab in the region of the supporting columns. Complementary nonlinear finite-element analyses of the overload and ultimate loadings are also presented. Results show that the posttensioned slab bridge supported a load-causing moment equivalent to 4.8 times the factored moment of an AASHTO MS 18 loading.
Mix & match electron beam & scanning probe lithography for high throughput sub-10 nm lithography (2013)

The prosperous demonstration of a technique able to produce features with single nanometer (SN) resolution could guide the semiconductor industry into the desired beyond CMOS era. In the lithographic community immense efforts are being made to develop extreme ultra-violet lithography (EUVL) and multiple-e-beam direct-write systems as possible successor for next generation lithography (NGL). However, patterning below 20 nm resolution and sub-10 nm overlay alignment accuracy becomes an extremely challenging quest. Herein, the combination of electron beam lithography (EBL) or EUVL with the outstanding capabilities of closed-loop scanning proximal probe nanolithography (SPL) reveals a promising way to improve both patterning resolution and reproducibility in combination with excellent overlay and placement accuracy. In particular, the imaging and lithographic resolution capabilities provided by scanning probe microscopy (SPM) methods touches the atomic level, which expresses the theoretical limit of constructing nanoelectronic devices. Furthermore, the symbiosis between EBL (EUVL) and SPL expands the process window of EBL (EUVL) far beyond state-of-the-art allowing SPL-based pre- and post-patterning of EBL (EUVL) written features at critical dimension level with theoretically nanometer precise pattern overlay alignment. Moreover, we can modify the EBL (EUVL) pattern before as well as after the development step. In this paper we demonstrate proof of concept using the ultra-high resolution molecular glass resist calixarene. Therefor we applied Gaussian E-beam lithography system operating at 10 keV and a home-developed SPL set-up. The introduced Mix and Match lithography strategy enables a powerful use of our SPL set-up especially as post-patterning tool for inspection and repair functions below the sub-10 nm critical dimension level.
Communication: Dissolution DNP reveals a long-lived deuterium spin state imbalance in methyl groups (2017)

We report the generation and observation of long-lived spin states in deuterated methyl groups by dissolution DNP. These states are based on population imbalances between manifolds of spin states corresponding to irreducible representations of the C<sub>3v</sub> point group and feature strongly dampened quadrupolar relaxation. Their lifetime depends on the activation energies of methyl group rotation. With dissolution DNP, we can reduce the deuterium relaxation rate by a factor up to 20, thereby extending the experimentally available time window. The intrinsic limitation of NMR spectroscopy of quadrupolar spins by short relaxation times can thus be alleviated.
Apolipoprotein E2 and E3, but Not E4, Promote Retinal Pathologic Neovascularization (2017)

These results demonstrate that ApoE2 and ApoE3, but not ApoE4, have proangiogenic effects, and the increased expression of ApoE in the vitreous humor of patients with PDR and DME indicates that ApoE2 and ApoE3 are involved in the development of retinal neovascularization in eyes.
Analysis of a phased array antenna based on phase-shifters with ferroelectric varactors (2003)

Results of theoretical analysis and experiments for separate parts and elements of a flat multilayer microstrip phased array antenna based on phase-shifters with ferroelectric varactors are presented. Mutual coupling and crosspolarization phenomena between radiator elements of two-layer patch aperture have been theoretically and experimentally studied. Calculated and measured data on the phase-shifter parameters are presented.
A Gelatin Microdroplet Platform for High‐Throughput Sorting of Hyperproducing Single‐Cell‐Derived Microalgal Clones (2018)

Microalgae are an attractive feedstock organism for sustainable production of biofuels, chemicals, and biomaterials, but the ability to rationally engineer microalgae to enhance production has been limited. To enable the evolution-based selection of new hyperproducing variants of microalgae, a method is developed that combines phase-transitioning monodisperse gelatin hydrogel droplets with commercial flow cytometric instruments for high-throughput screening and selection of clonal populations of cells with desirable properties, such as high lipid productivity per time traced over multiple cell cycles. It is found that gelatin microgels enable i) the growth and metabolite (e.g., chlorophyll and lipids) production of single microalgal cells within the compartments, ii) infusion of fluorescent reporter molecules into the hydrogel matrices following a sol-gel transition, iii) selection of high-producing clonal populations of cells using flow cytometry, and iv) cell recovery under mild conditions, enabling regrowth after sorting. This user-friendly method is easily integratable into directed cellular evolution pipelines for strain improvement and can be adopted for other applications that require high-throughput processing, e.g., cellular secretion phenotypes and intercellular interactions.
Bestimmung funktioneller Oberflächenparameter beim Mehrfach‐Streifenziehen von Stahl‐ und Aluminiumblechen in Zylinder/Blech/Zylinder Kontakt Geometrie (1998)

Abstract Mehrfach‐Streifenziehen von Karrosserieblechen in einer Zylinder/Blech/Zylinder Kontakt‐Geometrie eignet sich bei Blechumformung besonders zu der Simulation des Reibkontaktes zwischen glatten Werkzeugen und Blech mit kontrollierter Rauheit. Während solcher Versuche gilt das Hauptaugenmerk den Änderungen der Oberflächenrauheit auf den Plateaux, wo der Hauptanteil der Reib‐Scher‐Vorgänge während des Abgleitens stattfindet. Durch eine 3D‐Relokalisierung der im Tastschnitt‐Verfahren vermessenen Zone leiten wir statistische Parameter ab, die der mittleren Breite und Länge der tatsächlich tragenden Plateaux entsprechen. Die beobachtete Abtragung der Plateaux und der Anteil der Abriebspartikel als Drittkörper im Reibkontakt sind wesentlich stärker für Aluminium als für Stahl.
Source stirring technology for automotive antenna measurement (2008)

A statistically uniform E-field is created in a service environment by using the source stirring technology and the uniformity of the E-field is theoretically evaluated. With simulation of a service environment is shown, that the adoption of the source stirring technology can avoid the influence of the objects that distract the measurement results.