gebhart/patrepeval huggingface dataset:
Kinetic Study of Two Novel Enantiomeric Tricyclic β-Lactams Which Efficiently Inactivate Class C β-Lactamases (2001)

A detailed kinetic study of the interaction between two ethylidene derivatives of tricyclic carbapenems, Lek 156 and Lek 157, and representative beta-lactamases and D-alanyl-D-alanine peptidases (DD-peptidases) is presented. Both compounds are very efficient inactivators of the Enterobacter cloacae 908R beta-lactamase, which is usually resistant to inhibition. Preliminary experiments indicate that various extended-spectrum class C beta-lactamases (ACT-1, CMY-1, and MIR-1) are also inactivated. With the E. cloacae 908R enzyme, complete inactivation occurs with a second-order rate constant, k(2)/K', of 2 x 10(4) to 4 x 10(4) M(-1) s(-1), and reactivation is very slow, with a half-life of >1 h. Accordingly, Lek 157 significantly decreases the MIC of ampicillin for E. cloacae P99, a constitutive class C beta-lactamase overproducer. With the other serine beta-lactamases tested, the covalent adducts exhibit a wide range of stabilities, with half-lives ranging from long (>4 h with the TEM-1 class A enzyme), to medium (10 to 20 min with the OXA-10 class D enzyme), to short (0.2 to 0.4 s with the NmcA class A beta-lactamase). By contrast, both carbapenems behave as good substrates of the Bacillus cereus metallo-beta-lactamase (class B). The Streptomyces sp. strain R61 and K15 extracellular DD-peptidases exhibit low levels of sensitivity to both compounds.
Lead tetra-acetate oxidation of metallic copper to Cu2+: an unusual oxidation (1983)

Copper powder is directly oxidised to Cu2+ by lead tetra-acetate in a non-aqueous medium (CH2Cl2) under mild conditions (20–30 °C). In the presence of pyridine or a substituted pyridine, compounds of the type [{Cu(O2CMe)2L}2](L = pyridine, 2-, 3-, or 4-methylpyridine) are obtained in very high yields. The mononuclear complex [Cu(O2CMe)2(bipy)] is readily obtained by the oxidation of copper powder by Pb(O2CMe)4 in the presence of 2,2′-bipyridyl (bipy) by this simple procedure.
Recombinant Human Lactoferrin is Effective in the Treatment of <i>Helicobacter felis</i>-infected Mice (2000)

Recombinant human lactoferrin possesses in-vitro antibiotic and anti-inflammatory activity similar to the native form. It was tested for in-vivo activity in mice infected with the gastritis-inducing bacterium Helicobacter felis. A two-week course of treatment with lactoferrin was sufficient to partially reverse both infection-induced gastritis and the infection rate, and fully reverse gastric surface hydrophobicity changes. A comparison of lactoferrin with amoxicillin and standard triple therapy revealed no differences in infection rate. These results show that recombinant human lactoferrin is effective in a mouse model of Helicobacter infection, and support further testing of this promising agent for this application.
THE STRATIFIED SEMI-STATIONARY POPULATION (1947)

Journal Article THE STRATIFIED SEMI-STATIONARY POPULATION Get access S. VAJDA S. VAJDA Search for other works by this author on: Oxford Academic Google Scholar Biometrika, Volume 34, Issue 3-4, December 1947, Pages 243–254, https://doi.org/10.1093/biomet/34.3-4.243 Published: 01 December 1947
Obesity and hypertension (1994)

An increasing number of clinical trials have demonstrated that obese patients are more likely than lean individuals to be hypertensive. Moreover, both obesity and arterial hypertension have been identified as independent risk factors for cardiovascular disease. Pathophysiologically, obesity appears to have a major influence on the hemodynamic changes associated with hypertension. The available evidence suggests that at any given level of arterial pressure, obese hypertensive patients have a higher cardiac output and lower total peripheral resistance than do lean patients. Recent reports have indicated that obesity exerts a disparate effect on target organs in hypertension. Whereas at rest obesity seems to mitigate cardiovascular changes in the systemic vascular bed caused by hypertension, no such mitigation was observed in the renovasculature; left ventricular hypertrophy as a major cardiovascular risk factor was even exacerbated by the presence of obesity. The different hemodynamic patterns in obese hypertensive patients have recently been shown clinically relevant for treating hypertensive patients.
<b>Studies on Sphingolipids. VII. Synthesis and Configuration of Natural Sphingomyelins</b> (1962)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStudies on Sphingolipids. VII. Synthesis and Configuration of Natural SphingomyelinsDavid. Shapiro and H. M. FlowersCite this: J. Am. Chem. Soc. 1962, 84, 6, 1047–1050Publication Date (Print):March 1, 1962Publication History Published online1 May 2002Published inissue 1 March 1962https://pubs.acs.org/doi/10.1021/ja00865a036https://doi.org/10.1021/ja00865a036research-articleACS PublicationsRequest reuse permissionsArticle Views186Altmetric-Citations66LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Probing active‐site residues of pyranose 2‐oxidase from <i>Trametes multicolor</i> by semi‐rational protein design (2009)

D-Tagatose is a sweetener with low caloric and non-glycemic characteristics. It can be produced by an enzymatic oxidation of D-galactose specifically at C2 followed by chemical hydrogenation. Pyranose 2-oxidase (P2Ox) from Trametes multicolor catalyzes the oxidation of many aldopyranoses to their corresponding 2-keto derivatives. Since D-galactose is not the preferred substrate of P2Ox, semi-rational design was employed to improve the catalytic efficiency with this poor substrate. Saturation mutagenesis was applied on all positions in the active site of the enzyme, resulting in a library of mutants, which were screened for improved activity in a 96-well microtiter plate format. Mutants with higher activity than wild-type P2Ox were chosen for further kinetic investigations. Variant V546C was found to show a 2.5-fold increase of k(cat) with both D-glucose and D-galactose when oxygen was used as electron acceptor. Because of weak substrate binding, however, k(cat)/K(M) is lower for both sugar substrates compared to wild-type TmP2Ox. Furthermore, variants at position T169, i.e., T169S and T169N, showed an improvement of the catalytic characteristics of P2Ox with D-galactose. Batch conversion experiments of D-galactose to 2-keto-D-galactose were performed with wild-type TmP2O as well as with variants T169S, T169N, V546C and V546C/T169N to corroborate the kinetic properties determined by Michaelis-Menten kinetics.
Inhibition of δ-Aminolevulinic Acid Dehydrase by δ-Oximinolevulinic Acid (1960)

delta-Oximinolevulinic acid competitively inhibits delta-aminolevulinic acid dehydrase at low concentrations.
High-density EEG and independent component analysis mixture models distinguish knee contractions from ankle contractions (2011)

Decoding human motor tasks from single trial electroencephalography (EEG) signals can help scientists better understand cortical neurophysiology and may lead to brain computer interfaces (BCI) for motor augmentation. Spatial characteristics of EEG have been used to distinguish left from right hand motor imagery and motor action. We used independent component analysis (ICA) of EEG to distinguish right knee action from right ankle action. We recorded 264-channel EEG while 5 subjects performed a variety of knee and ankle exercises. An adaptive mixture independent component analysis (ICA) algorithm generated two distinct mixture models from a merged set of EEG signals (including both knee and ankle actions) without prior knowledge of the underlying exercise. The ICA mixture models parsed EEG signals into maximally independent component (IC) processes representing electrocortical sources, muscle sources, and artifacts. We calculated a spatially fixed equivalent current dipole for each IC using an inverse modeling approach. The fit of the models to the single trial EEG signals distinguished knee exercises from ankle exercise with 90% accuracy. For 3 of 5 subjects, accuracy was 100%. Electrocortical current dipole locations revealed significant differences in the knee and ankle mixture models that were consistent with the somatotopy of the tasks. These data demonstrate that EEG mixture models can distinguish motor tasks that have different somatotopic arrangements, even within the same brain hemisphere.
Abstract B100: Genomic copy number alterations associated with the early brain metastasis of non-small cell lung cancer (2013)

Abstract Frequent early development of systemic metastasis leads unfavourable clinical prognosis of the non-small cell lung cancer (NSCLC). Although the brain metastasis (BM) contributes significantly to the morbidity and mortality of NSCLC, relevant driver mechanisms are largely unknown. To elucidate genetic alterations and prognostic markers associated with early BM of NSCLC, we retrospectively collected 18 NSCLC patients with BM (12 adenocarcinomas (ADC) and 6 squamous cell carcinomas (SQCC)) whose surgical tissues of primary and/or metastatic tumors were preserved as formaldehyde fixed and paraffin embedded (FFPE) pathologic samples. When chromosomal copy number alterations (CNAs) of those FFPE samples were analysed by the Molecular Inversion Probe (MIP) technology, the most frequent CNAs detected in primary ADCs were gains of 3q, 5p, 5q, 6p, 8q, 9p, 11p, 15q, 17q and losses of 10q and 22q whereas primary SQCCs revealed gains in 4q and 12q and loss in 9q. Moreover, comparative MIP analysis on 11 matched cases demonstrated the fact that the BM carried the majority of genetic alterations present in the corresponding primary tumor but with a slightly higher frequency including gain of 9p11 and loss of 2p11 in ADCs. Especially, when primary 12 ADCs were analyzed depending on the pattern of BM to uncover predetermining signatures that can predict the risk of BM, selectively amplified regions of primary lung ADCs (5q35, 10q23 and 17q23-24) were identified as significantly associated with the development of early BM within 3 months after first diagnosis of primary tumor. Small interfering RNA silencing of ACTA2, which is one of several candidate genes revealed in this study, reduced the in vitro migratory and invasive potential of human ADC cell line PC14PE6-GFP tagged cells without affecting on proliferation. Although more validation is needed, the genetic signatures elucidated in this study shed light on the molecular mechanisms involved in BM and help to identify prognostic markers stratifying the subgroup at high risk of developing recurrence or distant metastasis within heterogeneous NSCLC patients. Citation Format: Hye Won Lee, Se Jeong Lee, Mi Young Song, Kyeung Min Joo, Do-Hyun Nam. Genomic copy number alterations associated with the early brain metastasis of non-small cell lung cancer. [abstract]. In: Proceedings of the AACR Special Conference on Tumor Invasion and Metastasis; Jan 20-23, 2013; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2013;73(3 Suppl):Abstract nr B100.
An integrated approach to investment appraisal for advanced manufacturing technology (1999)

This article presents an integrated approach combining the strategic, economic, and analytic justification methodologies to evaluate the tangible and intangible benefits for the selection of advanced manufacturing technology (AMT). A strategic approach called the manufacturing map is applied to determine the consistency among the proposed technology, the company objectives, and the manufacturing strategies. An analytic evaluation technique called the analytic hierarchy process (AHP) is also introduced. During the AHP analysis, an economic evaluation tool called the state-price net present value (SP-NPV) is applied to provide financial data. Finally, a budget/network chart is employed to monitor and outline the project in greater detail. © 1999 John Wiley & Sons, Inc.
Optical control over electron g factor and spin decoherence in (In,Ga)As∕GaAs quantum dots (2008)

We have studied the dependence of the electron in-plane g factor and spin decoherence time on the built-in electric field (Ei) at the position of a single layer of self-assembled (In,Ga)As∕GaAs quantum dots (QDs). Control of Ei is achieved by inducing screening charges in a p-i-n GaAs matrix with a continuous wave (cw) laser. Using a time-resolved pump-probe technique to measure the spin dynamics via the magneto-optical Kerr effect, we observe a large hole spin decoherence time of 440ps. Measurements as function of the cw laser power and, thus, of Ei show that the electron spin decay time in the QDs depends strongly on Ei and decreases from 310to110ps with increasing Ei. We attribute this effect to increasing tunneling rates of electrons out of the QDs at high Ei. We observe a slight increase of the electron g factor from 0.40±0.03 to 0.46±0.04 with increasing Ei, which might be a result of a changing wavefunction as a result of a different confinement potential due to Ei.
Preparation and properties of γ-Al2O3 films prepared by high temperature transformation of nanosized γ-AlOOH precursors (2007)

Congrès du 06.09.2007 au 07.09.2007
Bacterial Hen1 is a 3′ terminal RNA ribose 2′-O-methyltransferase component of a bacterial RNA repair cassette (2009)

Hen1 is an RNA ribose 2'-O-methyltransferase that modifies the 3' terminal nucleoside of eukaryal small regulatory RNAs. Here, we report that Hen1 homologs are present in bacterial proteomes from eight different phyla. Bacterial Hen1 is encoded by the proximal ORF of a two-gene operon that also encodes polynucleotide kinase-phosphatase (Pnkp), an RNA repair enzyme. Purified recombinant Clostridium thermocellum Hen1 is a homodimer of a 465-amino acid polypeptide. CthHen1 catalyzes methyl transfer from AdoMet to the 3' terminal nucleoside of an RNA oligonucleotide, but is unreactive with a synonymous DNA oligonucleotide or an RNA with a single 3'-terminal deoxyribose sugar. CthHen1 is optimally active at alkaline pH and dependent on manganese. Activity is inhibited by AdoHcy and abolished by mutations D291A and D316A in the putative AdoMet-binding pocket. The C-terminal fragment, Hen1-(259-465), comprises an autonomous monomeric methyltransferase domain.
Nitrification Treatability of Whole Microorganisms Immobilized by the PVA-Freezing Method (1992)

We used whole microorganisms immobilized by the PVA-freezing method (in order to prevent microorganism wash-out) to investigate the efficiency of nitrification. In general, denitrified or oxidized effluents are recycled to the nitrification tank to provide alkalinity control in biological nitrogen removal systems. We found that allowable volumetric loading during nitrification was different for the recycling and once-through operations, using a biofilm nitrogen removal system in a fixed bed. Then, the nitrification efficiency of a recycling operation in a biological nitrogen removal system was compared with that of a once-through operation, using immobilized microorganisms. The results showed that the ratio of allowable volumetric loading in the recycling operation was 60% of that in the once-through operation.
Repeater design to reduce delay and power in resistive interconnect (1998)

In large chips, the propagation delay of the data and clock signals can limit performance due to long resistive interconnect. The insertion of repeaters alleviates the quadratic increase in propagation delay with interconnect length while decreasing power dissipation by reducing short-circuit current, In order to develop a repeater design methodology, a timing model characterizing a complementary metal-oxide-semiconductor (CMOS) inverter driving a resistance-capacitance (RC) load is presented. The model is based on the Sakurai short-channel /spl alpha/-power law model of transistor operation. The inverter model is applied to the problem of repeaters to produce design expressions for determining the optimum number of uniformly sized repeaters to be inserted along a resistive interconnect line for reduced delay. For a wide variety of typical RC loads, this analytical repeater model exhibits a maximum error of 16% as compared to a dynamic circuit simulator (SPICE). The advantage of uniformly sized repeaters versus tapered-buffer repeaters is also investigated using the repeater model presented in this paper. It is shown that uniform repeaters remain advantageous over tapered buffers and tapered-buffer repeaters even with relatively small resistive RC loads. An expression for the short-circuit power dissipation of a repeater driving an RC load is presented, A comparison of the short-circuit power dissipation to the dynamic power dissipation in repeater chains and related power/delay tradeoffs are made.
The Opioid Peptide Dynorphin a Induces Leukocyte Responses via Integrin Mac-1 (α<sub>M</sub>β<sub>2</sub>, CD11b/CD18) (2015)

Together, the results identify dynorphins A and B as novel ligands for Mac-1 and suggest a role for the Dynorphin A-Mac-1 interactions in the induction of nonopiod receptor-dependent effects in leukocytes.
CORAL: Quantitative structure–activity relationship models for estimating toxicity of organic compounds in rats (2011)

For six random splits, one-variable models of rat toxicity (minus decimal logarithm of the 50% lethal dose [pLD50], oral exposure) have been calculated with CORAL software (http://www.insilico.eu/coral/). The total number of considered compounds is 689. New additional global attributes of the simplified molecular input line entry system (SMILES) have been examined for improvement of the optimal SMILES-based descriptors. These global SMILES attributes are representing the presence of some chemical elements and different kinds of chemical bonds (double, triple, and stereochemical). The "classic" scheme of building up quantitative structure-property/activity relationships and the balance of correlations (BC) with the ideal slopes were compared. For all six random splits, best prediction takes place if the aforementioned BC along with the global SMILES attributes are included in the modeling process. The average statistical characteristics for the external test set are the following: n = 119 ± 6.4, R(2) = 0.7371 ± 0.013, and root mean square error = 0.360 ± 0.037.
Dissociations between behavioural and functional magnetic resonance imaging-based evaluations of cognitive function after brain injury (2011)

Functional neuroimaging methods hold promise for the identification of cognitive function and communication capacity in some severely brain-injured patients who may not retain sufficient motor function to demonstrate their abilities. We studied seven severely brain-injured patients and a control group of 14 subjects using a novel hierarchical functional magnetic resonance imaging assessment utilizing mental imagery responses. Whereas the control group showed consistent and accurate (for communication) blood-oxygen-level-dependent responses without exception, the brain-injured subjects showed a wide variation in the correlation of blood-oxygen-level-dependent responses and overt behavioural responses. Specifically, the brain-injured subjects dissociated bedside and functional magnetic resonance imaging-based command following and communication capabilities. These observations reveal significant challenges in developing validated functional magnetic resonance imaging-based methods for clinical use and raise interesting questions about underlying brain function assayed using these methods in brain-injured subjects.
Discrete-time Frequency-Locked-Loop filters for parameters estimation of sinusoidal signals (2013)

The paper proposes a novel discretization technique for the design of discrete-time Frequency-Locked-Loop nonlinear (FLL) filters. Continuous-time nonlinear FLL filters have been proposed for their circuital simplicity and ability to provide unbiased estimates of all parameters of a time-varying sinusoidal signal, that is its instantaneous frequency, phase and amplitude. It is shown in the paper that standard discretization techniques fail to provide discrete-time FLL filters that exhibit the same good properties of their continuous-time counterparts. In particular, unlike continuous-time FLL filters, biased estimates are usually produced by these discrete-time FLL filters. In order to overcome this drawback, a Tustin discretization technique equipped with an adaptive pre-warping mechanism consisting of properly adjusting the pre-warping frequency on-line is shown to be sufficient to solve the problem. The properties of the resulting discrete-time FLL filters are fully analyzed. In particular, its semi-global exponential stability and convergence to the true values of the signal parameters has been established. A final example with comparisons with FLL filters synthesized by standard discretization techniques is provided for the assessment of the results.
Spinal Cord Swelling in Multiple Sclerosis (1981)

Three cases of subacutely progressive spinal paraparesis had myelographic evidence of spinal cord swelling suggesting the diagnosis of intramedullary tumour. The diagnosis of multiple sclerosis (MS) was made in each case with the aid of CSF protein electrophoresis, evoked responses and CT scan. Because acute MS may produce cord swelling simulating tumor or syrinx, these additional investigations may be necessary to make a correct diagnosis.
3-D digital imaging. Revealing the Location, Depth, and Concentration of Subsurface Pollutants (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXT3-D digital imaging. Revealing the Location, Depth, and Concentration of Subsurface PollutantsRalph L. Nichols, Brian B. Looney, and Jonathan E. HuddlestonCite this: Environ. Sci. Technol. 1992, 26, 4, 642–649Publication Date (Print):April 1, 1992Publication History Published online1 May 2002Published inissue 1 April 1992https://pubs.acs.org/doi/10.1021/es00028a001https://doi.org/10.1021/es00028a001research-articleACS PublicationsRequest reuse permissionsArticle Views41Altmetric-Citations3LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts