gebhart/patrepeval huggingface dataset:
Synthesis, Spectroscopy, and Hydroformylation Activity of Sterically Demanding, Phosphite‐Modified Cobalt Catalysts (2005)

Abstract The dinuclear complex [Co 2 (CO) 6 {P(O‐2,4‐ t ‐Bu 2 C 6 H 3 ) 3 } 2 ] ( 6 ) has been synthesised and fully characterised. X‐Ray crystal‐structure analysis revealed a trans ‐diaxial geometry, no bridging carbonyls, and CoCo and CoP bond lengths of 2.706(5) and 2.134(4) Å, respectively. The hydroformylation of pent‐1‐ene in the presence of 6 was studied at 120–180° at pressures between 20 and 80 bar Syngas. High‐pressure (HP) spectroscopy (IR, NMR) was used to detect potential hydride intermediates. HP‐IR Studies revealed the formation of [CoH(CO) 3 {P(O‐2,4‐ t ‐Bu 2 C 6 H 3 ) 3 }] ( 2 ) at ca. 105°, with no significant amount of [CoH(CO) 4 ] detectable. The intermediate 2 was synthesised and characterised. The formation of the undesired complex [CoH(CO) 2 {P(O‐2,4‐ t ‐Bu 2 C 6 H 3 ) 3 } 2 ] was completely suppressed due to the large cone angle of the sterically demanding phosphite.
Explore the Capability of ESPAR Antennas for Low Cost Communication (2012)

ESPAR antenna systems are composed of one active and several parasitic elements and by changing the characteristic of the parasitic elements on the antenna, the radiation pattern will also change. Such characteristic makes ESPAR antenna useful in many applications, e.g., single RF MIMO transmission. However, for higher modulation schemes like 16-QAM, more radiation patterns are needed which require a wider control range on the parasitic antenna load. In order to explore the capability of ESPAR antennas, negative resistance was proposed, but their values cannot be easily controlled both on the real and imaginary part. So a matching network is required to control the load value. This paper presents an idea of adjusting the parasitic ESPAR antenna loads with controllable passive elements. According to simulation, the control circuit consumes less than 1mW power on 64 patterns selection, which explores the capacity of ESPAR antenna for high order modulation applications.
Collagen nanofibres are a biomimetic substrate for the serum-free osteogenic differentiation of human adipose stem cells (2008)

Electrospinning has recently gained widespread attention as a process capable of producing nanoscale fibres that mimic native extracellular matrix. In this study, we compared the osteogenic differentiation behaviour of human adipose stem cells (ASCs) on a 3D nanofibre matrix of type I rat tail collagen (RTC) and a 2D RTC collagen-coated substrate, using a novel serum-free osteogenic medium. The serum-free medium significantly enhanced the numbers of proliferating cells in culture, compared to ASCs in traditional basal medium containing 10% animal serum, highlighting a potential clinical role for in vitro stem cell expansion. Osteogenic differentiation behaviour was assessed at days 7, 14 and 21 using quantitative real-time RT-PCR analysis of the osteogenic genes collagen I (Coll I), alkaline phosphatase (ALP), osteopontin (OP), osteonectin (ON), osteocalcin (OC) and core-binding factor-alpha (cbfa1). All genes were upregulated (>one-fold) in ASCs cultured on nanofibre scaffolds over 2D collagen coatings by day 21. Synthesis of mineralized extracellular matrix on the scaffolds was assessed on day 21 with Alizarin red staining. These studies demonstrate that 3D nanoscale morphology plays a critical role in regulating cell fate processes and in vitro osteogenic differentiation of ASCs under serum-free conditions.
An outbreak of acute bartonellosis (Oroya fever) in the Urubamba region of Peru, 1998. (1999)

During May 1998, we conducted a case-control study of 357 participants from 60 households during an outbreak of acute bartonellosis in the Urubamba Valley, Peru, a region not previously considered endemic for this disease. Blood and insect specimens were collected and environmental assessments were done. Case-patients (n = 22) were defined by fever, anemia, and intra-erythrocytic coccobacilli seen in thin smears. Most case-patients were children (median age = 6.5 years). Case-patients more frequently reported sand fly bites than individuals of neighboring households (odds ratio [OR] = 5.8, 95% confidence interval [CI] = 1.2-39.2), or members from randomly selected households > or = 5 km away (OR = 8.5, 95% CI = 1.7-57.9). Bartonella bacilliformis isolated from blood was confirmed by nucleotide sequencing (citrate synthase [g/tA], 338 basepairs). Using bacterial isolation (n = 141) as the standard, sensitivity, specificity, and positive predictive value of thin smears were 36%, 96%, and 44%, respectively. Patients with clinical syndromes compatible with bartonellosis should be treated with appropriate antibiotics regardless of thin-smear results.
Extremely High Incidence of Antibiotic Resistance in Clinical Isolates of Streptococcus pneumoniae in Hungary (1991)

An epidemiologic survey of antibiotic resistance among pneumococcal isolates collected during 1988 and 1989 in Hungary indicated that as many as 58% of all isolates and 70% of isolates from children were resistant to penicillin. These figures surpass even the highest values reported thus far for Spain and South Africa for the same period. Almost or more than 70% of the penicillin-resistant isolates were also resistant to tetracycline, erythromycin, and cotrimoxazole and approximately 30% to chloramphenicol. Intravenous administration of ampicillin (30 mg/kg) did not interfere with the growth in the cerebrospinal fluid of three resistant strains introduced into the rabbit model of experimental meningitis. No resistant strain showed beta-lactamase activity. A representative highly resistant strain contained altered penicillin-binding proteins (low penicillin affinities and abnormal molecular sizes) and was also resistant to the lytic and killing effects of penicillin.
Red alert for women's heart: the urgent need for more research and knowledge on cardiovascular disease in women: Proceedings of the Workshop held in Brussels on Gender Differences in Cardiovascular disease, 29 September 2010 (2011)

A recent report of the EuroHeart project has shown that women are still underrepresented in many cardiovascular clinical trials, while important gender differences are present within most areas of heart disease. As the burden of cardiovascular disease is increasing in middle-aged women relative to men, a more profound understanding is needed of the fundamental biological differences that exist between men and women. In the current review, we aim to address the need for more explanatory sex-specific cardiovascular research to be able to adapt existing guidelines for a better heart health in women.
Grundlagen der Umwandlung von Methan in ein reduktionsfähiges Gasgemisch (1971)

Kohlenwasserstoffe der allgemeinen Art CmHn eignen sich in dieser Form nicht zur Anwendung für Reduktionszwecke. Sie müssen vielmehr in technisch brauchbare, reduzierende Stoffe umgewandelt werden.
Differential Scanning Calorimetry of Reversed-Phase LC Packings (1983)

Differential scanning calorimetry is used to probe various aspects of the supramolecular structure of the alkyl surface of reversed-phase liquid chromatography packings. Three types of materials were employed in these studies: materials A, conventional chemically bonded C18 and C22 packings at coverages of about 2.0 to 2.5 micromoles/m2; materials B, the same chemically bonded packings as A, but supplemented with free C18 or C22n-alkanes at approximately 1:1 ratio; and materials C, high surface area SiO2 particulates to which C18 and C22n-alkanes were physically adsorbed at sufficient densities to yield closest-packed monolayer coverages. Not surprisingly, materials A failed to give distinct phase transitions. Here the density of the chains is such that interchain forces are too weak to provide an ordered phase. In contrast, materials B gave distinct phase transitions which occurred at a lower temperature, Tm, and with smaller enthalpy, ΔHm, than those observed for the pure alkanes. Materials C also gave distinct phase transitions, with values for Tm and ΔHm comparable to those of materials B. Finally, DSC was used to demonstrate the presence of a layer of organic modifier bound to the reversed phase.
The Tissue-Specific Transcription Factor Pit-1/GHF-1 Binds to the c-fos Serum Response Element and Activates c-fos Transcription (1999)

Pit-1, a POU domain-containing transcription factor, is involved in two functions in the pituitary: PRL and GH tissue-specific expression and somato-lactotroph cells expansion. To analyze the molecular basis of the latter function, we tested whether Pit-1 can directly transactivate expression of an early marker of cell cycle initiation, the c-fos gene. We show that Pit-1 overexpression in PC12 cells, which do not express Pit-1, increases c-fos expression. Moreover, cAMP-induced c-fos promoter activity is decreased in the somato-lactotroph cell line GH3 when Pit-1 expression is reduced by hybrid arrest with an antisense sequence complementary to Pit-1 cDNA. In contrast to hormonal genes regulation, where it has been shown that any Pit-1 phosphorylation site is involved, we show that the Pit-1 phosphorylation sites are required to allow increase of c-fos promoter activity by Pit-1. We further show, by gel shift analyses, that Pit-1 is able to specifically bind the serum response element sequence present within the c-fos promoter but with a lesser affinity than the Pit-1 response element. Taken together, these results demonstrate that the tissue-specific transcription factor Pit-1 is able to enhance expression of genes involved in cell cycle initiation, suggesting that this mechanism allows Pit-1 to increase somato-lactotroph cell proliferation.
Facile separations of enantiomers of chiral organometallic compounds with a Bakerbond Chiralcel HPLC column (1991)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTFacile separations of enantiomers of chiral organometallic compounds with a Bakerbond Chiralcel HPLC columnJames A. Ramsden, Charles M. Garner, and J. A. GladyszCite this: Organometallics 1991, 10, 5, 1631–1633Publication Date (Print):May 1, 1991Publication History Published online1 May 2002Published inissue 1 May 1991https://pubs.acs.org/doi/10.1021/om00051a066https://doi.org/10.1021/om00051a066research-articleACS PublicationsRequest reuse permissionsArticle Views122Altmetric-Citations23LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Crystal-contact engineering to obtain a crystal form of the Kelch domain of human Keap1 suitable for ligand-soaking experiments (2013)

Keap1 is a substrate adaptor protein for a Cul3-dependent ubiquitin ligase complex and plays an important role in the cellular response to oxidative stress. It binds Nrf2 with its Kelch domain and thus triggers the ubiquitinylation and degradation of Nrf2. Oxidative stress prevents the degradation of Nrf2 and leads to the activation of cytoprotective genes. Therefore, Keap1 is an attractive drug target in inflammatory diseases. The support of a medicinal chemistry effort by structural research requires a robust crystallization system in which the crystals are preferably suited for performing soaking experiments. This facilitates the generation of protein-ligand complexes in a routine and high-throughput manner. The structure of human Keap1 has been described previously. In this crystal form, however, the binding site for Nrf2 was blocked by a crystal contact. This interaction was analysed and mutations were introduced to disrupt this crystal contact. One double mutation (E540A/E542A) crystallized in a new crystal form in which the binding site for Nrf2 was not blocked and was accessible to small-molecule ligands. The crystal structures of the apo form of the mutated Keap1 Kelch domain (1.98 Å resolution) and of the complex with an Nrf2-derived peptide obtained by soaking (2.20 Å resolution) are reported.
Endogenous testosterone enhances growth hormone (GH)-releasing factor-induced GH secretion in vitro (1987)

The influence of endogenous gonadal steroids in male and female rats on basal and growth hormone-releasing factor (GRF)-stimulated GH secretion from perifused anterior pituitaries was studied. After 75 min of perifusion with basal medium, freshly dissected pituitaries were exposed to human GRF(1-44) (10 nmol/l) for 15 min. Neonatal (day 1-2) or prepubertal (day 25) gonadectomy of male rats suppressed baseline GH release (ng/min per mg dry weight) as well as GRF-stimulated GH release by 40-70%. This effect was slightly more pronounced in neonatally gonadectomized animals. In prepubertally gonadectomized male rats, the suppression of GH release was completely reversed by testosterone replacement therapy. In female rats, prepubertal gonadectomy did not affect GH secretion from perfused pituitaries. However, treatment of ovariectomized female rats with oestradiol reduced baseline and GRF-induced GH release to levels lower than those observed in sham-operated or vehicle-treated ovariectomized animals. The data suggest that testicular androgen secretion in adult male rats increases the pituitary GH release in response to GRF in vitro, whereas ovarian oestrogen secretion is of less importance for the GRF responsiveness of female rat pituitaries.
Forum: The Role and Future of Academic Journals (2015)

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The Preparation of Arbovirus Hemagglutinins by Sonication and Trypsin Treatment (1969)

For 28 of 31 arboviruses (34 strains) studied, satisfactory hemagglutinins were demonstrated after treatment of sucrose-acetone-extracted liver or brain preparations by either sonication alone or sonication plus trypsin treatment. The methods of antigen treatment are described, and the appearance of a “cellular agglutinin” under some conditions of trypsin treatment is reported. The procedure for hemagglutinin preparation seems to be consistent for any given virus, but variation is seen from virus to virus. Hemagglutination-inhibition testing of selected antigens indicates that sonication alone improves sensitivity, whereas further treatment with trypsin gives variable results.
ChemInform Abstract: Catalytic Asymmetric Synthesis of Cyclopentenones from Propargyl Malonates and Allylic Acetate by Successive Action of Homogeneous Palladium(II) and Cobalt on Charcoal Catalysts in a One‐Pot Reaction. (2002)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
5-Thiocyano-8-quinolinol (1953)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXT5-Thiocyano-8-quinolinolQuintus Fernando, J. F. Emery, and J. P. PhillipsCite this: J. Am. Chem. Soc. 1953, 75, 8, 1983–1985Publication Date (Print):April 1, 1953Publication History Published online1 May 2002Published inissue 1 April 1953https://doi.org/10.1021/ja01104a056RIGHTS & PERMISSIONSArticle Views129Altmetric-Citations1LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (373 KB) Get e-Alertsclose Get e-Alerts
P3‐243: Gene expression profiling of postmortem brain tissues affected by Alzheimer's disease (2008)

Most common form of Alzheimer's disease (AD) is late-onset AD (LOAD) (> 60 y.o.), accounting for roughly >95% of the case. Molecular mechanism of LOAD is not well understood. A high dense DNA microarray is available to analyze gene expression profiles of frozen tissues. Here we characterize total RNA isolated from human postmortem brain tissues prior to setting out identifying novel genes contributing to development or progression of LOAD. Two independent institutes of Japan, Tokyo Metropolitan Institute of Gerontology (N = 48) and Niigata University (N = 77), collected human postmortem brain tissues and quickly frozen at -80 degrees Celsius until use. We isolated total RNA from 125 brain tissues using the Trizol reagent and then the crude RNA was purified with an on-column DNase I treatment by a spin column purification system (Invitrogen). Quantification of the purified RNA was fluorometrically conducted using the Quant-iT RiboGreen RNA reagent (Molecular Probes). RNA quality was assessed by capillary gel electrophoresis (2100 BioAnalyzer, Agilent). An RNA integrity number (RIN, the best score is 10) and the ratio of 28S/18S ribosomal RNA were calculated with Expert software (Agilent). Eighty-four of the 125 brain tissues showed >7.0 of the RIN. The RIN (mean [SD], 7.3 [1.2]; range, 3.4 – 9.3) was strongly correlated with the 28S/18S ratio (mean [SD], 1.0 [0.3]; range, 0.3 – 1.7) (r-square = 0.6852, P < 0.0001). Both age at death (mean [SD], 79.7 y.o. [10.6]; range, 44 – 98 y.o.) and postmortem interval (mean [SD], 7.2 hr [9.9]; range, 1.0 – 64.1 hr) did not show any significant correlation between the RIN and the 28S/18S ratio. We have assessed total RNA isolated from postmortem human brain tissues, and observed that the RNA is high quality suitable for gene expression analysis. Gene expression profiling analysis is in progress using a DNA microarray, where over 47000 transcripts and variants spanning the entire human genome can be surveyed. It is expected that novel transcripts and its related proteins responsible for LOAD could be found through the expression profiling using the total RNA characterized here.
ChemInform Abstract: INTER‐ UND INTRAMOLEKULARE EN‐RK. ALIPHATISCHER NITROSO‐VERBINDUNGEN (1972)

Abstract Aus Caryophyllin (I) erhält man die Verbindungen (II) und (III).
UDP-Glucose: A Potential Signaling Molecule in Plants? (2018)

This perspective paper focuses on the most recent results suggesting a potential role for UDP-Glucose as a signaling molecule in plants. In animals, UDP-Glucose is well-established as an extracellular signaling molecule that is sensed by G-protein coupled receptors, activating several downstream defense mechanisms. Recent studies have shown that abnormal growth occurred in both vegetative and reproductive tissue of plants with reduced UDP-Glucose levels, and this could be rescued by exogenous UDP-Glucose. In plants with increased biomass accumulation, the genes involved in UDP-Glucose production were up-regulated. However, excessive endogenous accumulation of UDP-Glucose induced programmed cell death (PCD), and this could also be obtained by exogenous UDP-Glucose application. Plants with decreased UDP-glucose were insensitive to pathogen induced PCD. We speculate that UDP-Glucose acts as an extracellular signaling molecule in plants, and that it may be perceived as a damage-associated molecular pattern.
Hyperosmolarity enhances the lung capillary barrier (2003)

Although capillary barrier deterioration underlies major inflammatory lung pathology, barrier-enhancing strategies are not available. To consider hyperosmolar therapy as a possible strategy, we gave 15-minute infusions of hyperosmolar sucrose in lung venular capillaries imaged in real time. Surprisingly, this treatment enhanced the capillary barrier, as indicated by quantification of the capillary hydraulic conductivity. The barrier enhancement was sufficient to block the injurious effects of thrombin, TNF-α, and H2O2 in single capillaries, and of intratracheal acid instillation in the whole lung. Capillary immunofluorescence indicated that the hyperosmolar infusion markedly augmented actin filament formation and E-cadherin expression at the endothelial cell periphery. The actin-depolymerizing agent latrunculin B abrogated the hyperosmolar barrier enhancement as well as the actin filament formation, suggesting a role for actin in the barrier response. Furthermore, hyperosmolar infusion blocked TNF-α–induced P-selectin expression in an actin-dependent manner. Our results provide the first evidence to our knowledge that in lung capillaries, hyperosmolarity remodels the endothelial barrier and the actin cytoskeleton to enhance barrier properties and block proinflammatory secretory processes. Hyperosmolar therapy may be beneficial in lung inflammatory disease.
Abstract 1934: Predictors of Long-Term Success Following Balloon Angioplasty of Native Coarctation (2006)

BACKGROUND: Balloon angioplasty (BA) of native coarctation (CoA) is an accepted therapy, however, long-term results and predictors of success are limited. OBJECTIVES: To examine the long-term outco...
Hydrothermal Origin for Carbonate Globules in ALH84001 by Analogy with Similar Carbonates from Spitsbergen (Norway) (2002)

Basalts and xenoliths from Spitsbergen (Norway) contain carbonate globules nearly identical to those in ALH84001. The Spitsbergen globules formed from hydrothermal waters by analogy, so did those in ALH84001. Additional information is contained in the original extended abstract.
Natural Killer Cell Immunodeficiency in HIV Disease is Manifest by Profoundly Decreased Numbers of CD16 + CD56+ Cells and Expansion of a Population of CD16dim CD56- Cells with Low Lytic Activity (1995)

Natural killer (NK) cells were enumerated by three-color immunofluorescence in 255 uninfected and 399 human immunodeficiency virus-infected adults. Several dramatic alterations were observed. First, the median number and percentage of CD16+CD56+ NK cells, the subset that comprises > 90% of the NK cells in healthy adults, were severely decreased (median, 175/mm3 in uninfected controls; 63/mm3 in HIV-infected non-AIDS subjects). Even subjects with > 800 CD4+ cells/mm3 had decreased CD16+CD56+ NK cell levels (97/mm3). Second, the number of CD16+CD56- cells, an NK population that is rare in healthy adults, was elevated (median, 20/mm3 in uninfected controls; 64/mm3 in HIV-seropositive non-AIDS subjects). Third, the expression of CD16 on the NK cells was markedly reduced; some CD56+ cells and virtually all CD56- cells were CD16dim. Fourth, fluorescence-activated cell-sorting studies revealed little NK- or antibody-dependent cellular cytotoxic activity in the CD16dimCD56- cell population. These results indicate that the pathogenesis of HIV disease includes numerical alterations in subpopulations of NK cells. A better understanding of how HIV infection causes this aspect of pathogenesis is needed.