gebhart/patrepeval huggingface dataset:
Liposome-Based Immunosensors. 1. Influence of Hapten Spacer Length on Liposome Binding Efficiency (1996)

Abstract The effect of spacers on the binding of atrazine-tagged liposomes to anti-atrazine antibodies was studied. Atrazine derivatives with alkyl spacers of different length (C4, C6, C8 and C12) were synthezised and conjugated to dipalmitoyl phosphatidyl ethanolamine. The conjugates were incorporated into liposomal membranes of DPPC/DPPG/cholesterol containing ascorbic acid as entrapped marker. Tagged liposomes were bound to a monoclonal anti-atrazine antibody immobilized on a nitrocellulose membrane, destroyed by the addition of surfactant and the marker was detected electrochemically using printed thick-film electrodes. Our results show that liposomes incorporating a hapten with short alkyl spacer binds better to the immobilized antibody. These results will allow optimization of liposomes as an enhancement system in electrochemical or optical biosensors.
Crystal Structure of Form III of Syndiotactic Poly(p-methylstyrene) (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCrystal Structure of Form III of Syndiotactic Poly(p-methylstyrene)Claudio De Rosa, Vittorio Petraccone, Francesco Dal Poggetto, Gaetano Guerra, Beniamino Pirozzi, Maria Laura Di Lorenzo, and Paolo CorradiniCite this: Macromolecules 1995, 28, 16, 5507–5511Publication Date (Print):July 1, 1995Publication History Published online1 May 2002Published inissue 1 July 1995https://pubs.acs.org/doi/10.1021/ma00120a014https://doi.org/10.1021/ma00120a014research-articleACS PublicationsRequest reuse permissionsArticle Views97Altmetric-Citations25LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Development of a force field for Li<sub>2</sub>SiF<sub>6</sub> (2005)

Abstract A force field has been developed for Li 2 SiF 6 for subsequent use in Molecular Dynamics (MD) simulations involving Li + and SiF ions in a polymer electrolyte host. Both ab initio calculations and available empirical data have been used. The force field has been verified in simulations of the crystal structure of Li 2 SiF 6 in two different space groups: P321 and P $\bar{3}$ m1 . The use of MD simulation to assess the correct space group for Li 2 SiF 6 shows that it is probably P321. © 2005 Wiley Periodicals, Inc. J Comput Chem 26: 716–724, 2005
The E-SCREEN Assay as a Tool to Identify Estrogens: An Update on Estrogenic Environmental Pollutants (1995)

This paper was presented at the Symposium on Estrogens in the Environment, III: Global Health Implications held 9-11 January 1994 in Washington, DC.
Overproduction and Purification of the <i>Aeromonas hydrophila</i> CphA Metallo-β-Lactamase Expressed in <i>Escherichia coli</i> (1996)

The Aeromonas hydrophila CphA metallo-beta-lactamase was overexpressed in a soluble secreted form in Escherichia coli using a T7 RNA polymerase-based expression system, and a simple protocol based on a single cation-exchange chromatographic step was developed, which is suitable for rapid purification of the overexpressed enzyme from E. coli lysates. A yield of up to 30 micrograms of purified enzyme per milliliter of culture was obtained. The purified enzyme preparation showed properties identical to those previously reported in the literature.
Plasma apolipoprotein concentrations in young adults with a parental history of premature coronary heart disease and in control subjects. The EARS Study. European Atherosclerosis Research Study. (1994)

The European Atherosclerosis Research Study (EARS) is a multicenter collaborative project within the European community. Its main objective is to study in young individuals the biological expression of a paternal history of premature coronary heart disease and to analyze the relative contribution of genetic and environmental factors to this expression. This study was carried out in 14 centers in 11 European countries, where the offspring of fathers who suffered a documented myocardial infarction before the age of 55 years (cases) were compared with age- and sex-matched control subjects. Plasma apolipoproteins A-I, B, A-II, A-IV, and E and lipoprotein (Lp) A-I lipoparticles were measured in this student population. Comparison of the values between cases and control subjects showed significantly higher apo B levels in cases compared with control subjects, and these differences were homogeneous throughout Europe. Regional differences were observed for apo E levels with an increasing north-south gradient, which was inversely related to that observed for triglycerides. A stepwise regression analysis including the lipid and apolipoprotein variables showed that apo B and triglycerides were the strongest discriminators between offspring of fathers with premature coronary heart disease and control subjects.
Mechanism of Inactivation in Aerosols of Bacteriophage T1 (1972)

Summary The inactivation of Escherichia coli bacteriophage T1 was determined after formation of aerosols from solutions in NaCl, NaBr, NaNO3 and Na2SO4. In all cases, survival varied rapidly with relative humidity, with a minimum near the relative humidity corresponding to a saturated solution of the salt. Survival was better when the aerosol was formed at low initial salt concentrations. Broth protected against aerosol-inactivation. Phage particles surviving surface-inactivation by shaking in solution were resistant to aerosol-inactivation. The inactivation process in the range of humidities where the aerosol particles are fluid seems mainly to be due to surface-inactivation.
Mini-Open Rotator Cuff Repair (2000)

Summary: Arthroscopically assisted or mini-open repair has become a popular method of treating full-thickness tears of the rotator cuff. This technique combines an arthroscopic subacromial decompression with an open tendon repair using a small deltoid split approach. The deltoid origin is preserved because the anterior acromioplasty is performed arthroscopically. The mini-open technique integrates arthroscopic and open methods for the optimal treatment of rotator cuff tears. This article presents the indications, technique, and results of the mini-open technique.
Structural specificity of high affinity [3H]-glutamate uptake in striatal homogenates (1979)

Journal Article Structural specificity of high affinity [3H]-glutamate uptake in striatal homogenates Get access Steven R Vincent, Steven R Vincent Kinsmen Laboratory of Neurological Sciences, Department of Psychiatry, University of British Columbia, Vancouver, B.C., Canada V6T 1W5 Search for other works by this author on: Oxford Academic Google Scholar Edith G McGeer Edith G McGeer Kinsmen Laboratory of Neurological Sciences, Department of Psychiatry, University of British Columbia, Vancouver, B.C., Canada V6T 1W5 Correspondence: Kinsmen Laboratory of Neurological Sciences, Department of Psychiatry, University of British Columbia, Vancouver, B.C., Canada V6T 1W5 Search for other works by this author on: Oxford Academic Google Scholar Journal of Pharmacy and Pharmacology, Volume 31, Issue 1, September 1979, Pages 703–704, https://doi.org/10.1111/j.2042-7158.1979.tb13633.x Published: 12 April 2011 Article history Received: 07 May 1979 Published: 12 April 2011
Effect of Grape Seed Proanthocyanidin–Gelatin Colloidal Complexes on Stability and in Vitro Digestion of Fish Oil Emulsions (2015)

The colloidal complexes composed of grape seed proanthocyanidin (GSP) and gelatin (GLT), as natural antioxidants to improve stability and inhibit lipid oxidation in menhaden fish oil emulsions, were evaluated. The interactions between GSP and GLT, and the chemical structures of GSP/GLT self-assembled colloidal complexes, were characterized by isothermal titration calorimetry (ITC), circular dichroism (CD), and Fourier transform infrared spectroscopic (FTIR) studies. Fish oil was emulsified with GLT to obtain an oil-in-water (o/w) emulsion. After formation of the emulsion, GLT was fixed by GSP to obtain the GSP/GLT colloidal complexes stabilized fish oil emulsion. Menhaden oil emulsified by GSP/GLT(0.4 wt %) colloidal complexes yielded an emulsion with smaller particles and higher emulsion stability as compared to its GLT emulsified counterpart. The GSP/GLT colloidal complexes inhibited the lipid oxidation in fish oil emulsions more effectively than free GLT because the emulsified fish oil was surrounded by the antioxidant GSP/GLT colloidal complexes. The digestion rate of the fish oil emulsified with the GSP/GLT colloidal complexes was reduced as compared to that emulsified with free GLT. The extent of free fatty acids released from the GSP/GLT complexes stabilized fish oil emulsions was 63.3% under simulated digestion condition, indicating that the fish oil emulsion was considerably hydrolyzed with lipase.
Patellectomy and osteoarthritis: arthroscopic findings following previous patellectomy (1993)

We reviewed the arthroscopic findings in 16 knees in which a patellectomy had been performed 1-47 years previously (average 15.4 years). The study group consisted of 13 women and 3 men with an average age of 46.3 years (range 22-64 years). The knee symptoms had failed to respond to non-operative treatment and the treating surgeon (R.J.B.) had determined that arthroscopic examination was warranted. Fifteen of the 16 patients had articular cartilage damage, particularly in the medial compartment and trochlear groove. The severity of articular cartilage damage correlated with time since patellectomy, but not with patient age. Apart from the previous patellectomy, no predisposing factors could be identified to account for the frequency and severity of tibiofemoral osteoarthritis in this relatively young group of patients. Our findings suggest that there may be an association between patellectomy and the subsequent development of osteoarthritis.
Visualization methods for statistical analysis of microarray clusters (2005)

Incorporating relevant statistical information into data visualizations is key for analysis of large biological datasets, particularly because of high levels of noise and the lack of a gold-standard for comparisons. We developed several new visualization techniques and demonstrated their effectiveness for evaluating cluster quality and relationships between clusters.
<i>In vitro</i> differentiation of <i>Runx3</i><sup>−/–</sup><i>p53</i><sup>−/–</sup> gastric epithelial cells into intestinal type cells (2008)

We have reported that a lack of RUNX3 function is causally associated with gastric carcinogenesis. We have also presented evidence that loss of Runx3 may be related to the genesis of intestinal metaplasia because expression of RUNX3 is reduced in some intestinal metaplasias, and some Runx3(-/-)p53(-/-) gastric epithelial cells differentiate into intestinal type cells in vivo. Recently several reports have indicated that blood cells play important roles in the gastric carcinogenesis. In the present study, we therefore examined whether Runx3(-/-)p53(-/-) gastric epithelial cells differentiate autonomously into intestinal type cells, or whether the presence of other cells is necessary for the differentiation in vitro. When Runx3(-/-)p53(-/-) gastric epithelial cells were cultured with collagen gels, they did not exhibit any morphogenesis and differentiated poorly. When cultured with fetal mouse gastric mesenchymes, the cells formed glandular structures and differentiated into surface mucous cells, but differentiation of intestinal type cells was never observed. When cultured with Matrigel, the cells formed glandular structures, and some cells differentiated into intestinal type cells in vitro. Reverse transcription-polymerase chain reaction analysis showed that the cells expressed stomach-specific genes, and their levels decreased gradually during the culture. The cells expressed some intestine-specific genes weakly at the start of culture, and their levels were increased with time in culture. We therefore conclude that Runx3(-/-)p53(-/-) gastric epithelial cells differentiate into intestinal type cells in combination with Matrigel in the absence of other cell types. Extracellular matrix, not blood cells, may play a role in the genesis of intestinal metaplasia.
Aspiration techniques for bronchoalveolar lavage in translational respiratory research: Paving the way to develop novel therapeutic moieties (2017)

Bronchoalveolar lavage (BAL) is a simple, yet informative tool in understanding the immunopathology of various lung diseases <i>via</i> quantifying various inflammatory cells, cytokines and growth factors. At present, this traditional method is often blended with several robust and sophisticated molecular and biological techniques sustaining the significance and longevity of this technique. Crucially, the existence of slightly distinct approaches and variables employed at different laboratories around the globe in performing BAL aspiration indeed demands an utmost need to optimize and develop an effective, cost-effective and a reproducible technique. This mini review will be of importance to the biological translational scientist, particularly respiratory researchers in understanding the fundamentals and approaches to apply and consider with BAL aspiration techniques. This will ensure generating a meaningful and clinically relevant data which in turn accelerate the development of new and effective therapeutic moieties for major respiratory conditions.
Uncoupling of Gating Charge Movement and Closure of the Ion Pore During Recovery from Inactivation in the Kv1.5 Channel (2002)

Both wild-type (WT) and nonconducting W472F mutant (NCM) Kv1.5 channels are able to conduct Na(+) in their inactivated states when K(+) is absent. Replacement of K(+) with Na(+) or NMG(+) allows rapid and complete inactivation in both WT and W472F mutant channels upon depolarization, and on return to negative potentials, transition of inactivated channels to closed-inactivated states is the first step in the recovery of the channels from inactivation. The time constant for immobilized gating charge recovery at -100 mV was 11.1 +/- 0.4 ms (n = 10) and increased to 19.0 +/- 1.6 ms (n = 3) when NMG(+)(o) was replaced by Na(+)(o). However, the decay of the Na(+) tail currents through inactivated channels at -100 mV had a time constant of 129 +/- 26 ms (n = 18), much slower than the time required for gating charge recovery. Further experiments revealed that the voltage-dependence of gating charge recovery and of the decay of Na(+) tail currents did not match over a 60 mV range of repolarization potentials. A faster recovery of gating charge than pore closure was also observed in WT Kv1.5 channels. These results provide evidence that the recovery of the gating elements is uncoupled from that of the pore in Na(+)-conducting inactivated channels. The dissociation of the gating charge movements and the pore closure could also be observed in the presence of symmetrical Na(+) but not symmetrical Cs(+). This difference probably stems from the difference in the respective abilities of the two ions to limit inactivation to the P-type state or prevent it altogether.
Realization of artificial Kondo lattices in nanostructured arrays (2011)

The interplay of magnetic energies in a Kondo lattice is the underlying physics of a heavy fermion system. Creating an artificial Kondo lattice system by localizing the moments in an ordered metallic array provides a prototype system to tune and study the energetic interplay while avoiding the complications introduced by random alloying of the material. In this paper, we create a Kondo lattice system by fabricating a hexagonally ordered nanostructured array using niobium as the host metal and cobalt as the magnetic constituent. Electrical transport measurements and magnetoresistivity measurements of these artificial lattices show that the competing exchange coupling properties can be easily tuned by controlling the impurity percentage. These artificial Kondo lattice systems enable the exploration of an artificial superconductor which should lead to a deep understanding of the role of magnetism in unconventional superconductors.
Zur Kenntnis des Narciclasins (1975)

Abstract Aus Narciclasin ( 2a ) wird das Acetonid 8a und dessen Oxidationsprodukt 10 dargestellt. 10 wird leicht zu 11 isomerisiert. Das bei der katalytischen Hydrierung von 2a anfallende Gemisch der Dihydronarciclasine wird über die Tetraacetate in die cis ‐ und trans ‐Verbindungen 14a und 15a aufgetrennt. Als Nebenprodukt der katalytischen Hydrierung entsteht Isonarciclasin ( 16a ). Mit Hilfe der NMR‐Spektren von 15b und 16b läßt sich die relative Konfiguration des Narciclasins entsprechend 2a beweisen. Lycoricidin und Margetin sind identisch und haben die Konfiguration 2b . Über einige biologische Teste wird berichtet.
Solving the interior problem of computed tomography using<i>a priori</i>knowledge (2008)

The case of incomplete tomographic data for a compactly supported attenuation function is studied. When the attenuation function is a priori known in a subregion, we show that a reduced set of measurements is enough to uniquely determine the attenuation function over all the space. Furthermore, we found stability estimates showing that reconstruction can be stable near the region where the attenuation is known. These estimates also suggest that reconstruction stability collapses quickly when approaching the set of points that are viewed under less than 180 degrees. This paper may be seen as a continuation of the work "Truncated Hilbert transform and Image reconstruction from limited tomographic data" that was published in Inverse Problems in 2006. This continuation tackles new cases of incomplete data that could be of interest in applications of computed tomography.
Bi-directional Analysis of Tau Propagation between Braak Stages along Functional Connectivity Pathways (S9.001) (2019)

To determine the relationship between pathological tau deposition in Alzheimer’s disease (AD), functional connectivity in the healthy brain, and the Braak stages of neuropathological tau deposition.
Radiosensitization of androgen receptor (AR)-positive triple-negative breast cancer (TNBC) cells using seviteronel (SEVI), a selective CYP17 lyase and AR inhibitor. (2017)

e12102 Background: Increased rates of locoregional recurrence have been observed in TNBC despite chemotherapy and radiation (RT). A novel radiosensitizer screen nominated the AR as a promising target for treatment of radioresistant breast cancer, including TNBC. We assessed in vitro activity of SEVI (VT-464), a selective CYP17 lyase and AR inhibitor, as a potential radiosensitizer in AR+ TNBC model. Methods: Clonogenic survival assays were used to determine the intrinsic RT sensitivity of 21 breast cancer cell (BCC) lines. IC50 values were determined for 130 clinically available compounds and correlation coefficients were calculated using IC50 values and SF-2Gy. Gene expression was measured using RNA Seq or qRT-PCR and protein expression was measured using RPPA arrays. AR function was assessed using functional inhibition with SEVI in MDA-MB-453, ACC-460, SUM-185 (all three AR+ TNBC), MDA-MB-231 (AR- TNBC), and T47D (AR- ER+) BCC lines. Double-stranded DNA (dsDNA) break repair was assessed with γH2AX foci counting. Results: Our novel radiosensitizer screen identified the activity of bicalutamide, an AR antagonist, in RT-resistant BCC lines (R2 = 0.46, p-value < 0.01). Heterogeneity in AR expression was identified in human BCC lines. There was a strong correlation between AR RNA expression and protein expression across all BC intrinsic subtypes. AR inhibition using SEVI induced radiation sensitivity in vitro with an enhancement ratio (ER) of 1.24-1.62 in three different AR+ TNBC lines. No such radiosensitization was seen in AR(-) TNBC or ER+, AR(-) BCC lines. Radiosensitization was at least partially dependent on impaired dsDNA break repair with significant delays in dsDNA break repair at 16 and 24 hours in all AR+ TNBC lines examined (p-value < 0.01). Conclusions: Our results implicate the AR as a mediator of radioresistance in breast cancer and support the rationale for developing seviteronel as a novel radiosensitizing agent in AR+ TNBC.
GridConnected CapacitorTapped MultiModule Voltage Source Converter (2018)

In this work, a new dc-ac converter is proposed for grid integration purposes, namely, capacitor-tapped multi-module dc-ac voltage source converter. The main advantages of the proposed converter when compared with the conventional dc-ac modular multilevel converter (assuming the same voltage rating of semiconductor devices) are: (i) employment of a lower number of semiconductor devices, but with a higher current rating hence it provides operation with a lower number of gate driver circuits, and (ii) employment of a lower number of dc capacitors which reduces the number of measured variables, which affects positively the cost and reduces the computational burden of the employed controller.
Wang-Landau Monte Carlo formalism applied to ferroelectrics (2016)

The Wang-Landau Monte Carlo algorithm is implemented within an effective Hamiltonian approach and applied to ${\mathrm{BaTiO}}_{3}$ bulk. The density of states obtained by this approach allows a highly accurate and straightforward calculation of various thermodynamic properties, including phase transition temperatures, as well as polarization, dielectric susceptibility, specific heat, and electrocaloric coefficient at any temperature. This approach yields rather smooth data even near phase transitions and provides direct access to entropy and free energy, which allow us to compute properties that are typically unaccessible by atomistic simulations. Examples of such latter properties are the nature (i.e., first order versus second order) of the phase transitions for different supercell sizes and the thermodynamic limit of the Curie temperature and latent heat.
Failure to produce greater than additive sister chromatid exchange induction with X rays and BCNU. (1988)

In 1984, Tofilon and Deen reported that X irradiation of 9L cells immediately after treatment with 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) produced greater than additive induction of sister chromatid exchanges (SCEs) compared to the sum of SCEs induced by each agent alone [Radiat. Res. 97, 171-177 (1984)]. We (C.P.S., D.F.D.) repeated experiments conducted by Tofilon and Deen, scored SCEs in a blind manner, and were unable to reproduce the original findings. Instead, we found that X rays and BCNU induced SCEs in an additive manner. The slides prepared in the original study are extant; they were coded and recounted blind. Data obtained in this reevaluation do not substantiate the previous report. We conclude that the original findings were apparently the result of bias introduced in the SCE scoring process. Our experience with the SCE assay emphasizes the need to recode SCE slide preparations and count SCEs blind.