gebhart/patrepeval huggingface dataset:
Yeast profilin complements profilin deficiency in transgenic tomato fruits and allows development of hypoallergenic tomato fruits (2010)

ABSTRACT Gene silencing of Lyc e 1 leads to reduced allergenicity of tomato fruits but impaired growth of transgenic tomato plants. The aim of the study was to restore growth of Lyc e 1‐deficient tomato plants while retaining reduced allergenicity by simultaneous complementation of profilin deficiency by expression of nonallergenic yeast profilin. Transgenic plants were generated and tested by RT‐PCR and immunoblotting;allergenicity of yeast profilin and transgenic fruits was investigated by IgE binding, basophil activation, and skin‐prick tests. Lyc e 1 content of transgenic tomato fruits was <5% of that of wild‐type plants, causing significantly reduced IgE antibody binding. Simultaneous coexpression of yeast profilin restored growth and biomass production almost to wildtype levels. Yeast profilin, sharing 32.6% amino acid sequence identity with Lyc e 1, displayed low IgE‐binding capacity and allergenic potency. Among 16 tomato‐allergic patients preselected for sensitization to Lyc e 1, none showed significant reactivity to yeast profilin. Yeast profilin did not induce mediator release, and coexpression of yeast profilin did not enhance the allergenicity of Lyc e 1‐reduced fruits. Simultanous coexpression of yeast profilin allows silencing of tomato profilin and generation of viable plants with Lyc e 1‐deficient tomato fruits. Therefore, a novel approach to allergen avoidance, genetically modified foods with reduced allergen accumulation, can be generated even if the allergen fulfills an essential cellular function in the plant. In summary, our findings of efficiently complementing profilin‐defi‐cient tomato plants by coexpression of low allergenic yeast profilin demonstrate the feasibility of creating low‐allergenic food even if the allergen fulfills essential cellular functions.—Le, L. Q., Mahler, V., Scheu‐rer, S., Foetisch, K., Braun, Y., Weigand, D., Enrique, E., Lidholm, J., Paulus, K. E., Sonnewald, S., Vieths, S., Sonnewald, U. Yeast profilin complements profilin deficiency in transgenic tomato fruits and allows development of hypoallergenic tomato fruits. FASEB J. 24, 4939–4947 (2010). www.fasebj.org
Turbulence strength estimation and super-resolution from an arbitrary set of atmospherically degraded images (2006)

<sup></sup>In remote sensing, atmospheric turbulence and aerosols limit the image quality. For many practical cases turbulence is shown to be dominant, especially for horizontal close-to-earth imaging in hot environments. In a horizontal long-range imaging it is usually impractical to measure path-averaged refractive index structure constant C<sub>n</sub><sup>2</sup> (which characterizes the turbulence strength) with conventional equipment. In this paper we propose a method for estimation of C<sub>n</sub><sup>2</sup> based just on the available recorded turbulence-degraded image sequence. The method exploits the turbulence-induced image "dancing". C<sub>n</sub><sup>2</sup> is extracted from the estimated image shifts variance. Experimental comparison with C<sub>n</sub><sup>2</sup> measurements using a scintillometer shows reliable estimation results. We also estimate image motion with sub-pixel accuracy for the purpose of obtaining a high-resolution image by applying a simple super-resolution procedure. Results of super-resolution for real imagery are presented.
Estimation of Montmorillonite Swelling Pressure: A Molecular Dynamics Approach (2015)

The swelling pressure of montmorillonite is an important factor in designing the bentonite buffer for geo-disposal of nuclear waste. A method to predict the swelling pressure of montmorillonite based on molecular dynamic simulations has been presented. A model structure of a Na-montmorillonite clay was put into contact with pure water, sodium chloride, and calcium chloride solutions. By imitating the experimental determination of the swelling pressure of bentonite clay, a spring sensor was designed to probe the swelling pressure. The modeled swelling pressure and its sensitivity to the dry density of clay were found to be in agreement with experimental data. The model provides an atomic level computational approach for the prediction of swelling pressure of bentonite clay in the context of geo-disposal of nuclear waste.
[The organization of the cortex in ciliates: an example of inheriting an acquired trait]. (1990)

Observations and experiments concerning morphology, morphogenesis and regeneration of ciliates impose the conclusion that the cortex plays a determining role in the arrangement of kinetics and that its organization is not commanded by the genome but behaves as an acquired hereditary character.
A model-view separation architecture for GUI application components (2005)

Graphical user interface (GUI) application component is a compound component, which consists of fine-grained components (such as GUI widgets) and specific logic. GUI application components fabricated by conventional techniques expose low extensibility because of the incomplete separation between the application logic part and GUI part inside the components. In this paper, we propose a new component architecture, "BeaM", which realizes complete separation between both parts inside the GUI application components. We have implemented a development environment corresponding to the proposed architecture in Java language. As a result of experimental evaluations, it is found that BeaM is useful to develop GUI application components as JavaBeans components with high extensibility.
A new procedure to assess the hot rolling work roll materials (2007)

CRM developed a new cost effective procedure to have a more accurate assessment of roll performance related to oxide formation, friction level and wear for hot rolling. The procedure consists of inserting different work roll materials in pilot work rolls to perform specific continuous pilot hot rolling campaigns with a detailed analysis. This gives a direct comparison between the different materials tested in a real rolling environment. Furthermore it reduces the technical risk related to the introduction of new work roll grades and speeds up the roll development.
Isolation of muscle derived stem cells from rat and its smooth muscle differentiation [corrected]. (2004)

We investigated whether stem cells (MDSC) from primary cultures of rat skeletal muscle can differentiate into the smooth muscle lineage in response to vascular endothelial growth factor (VEGF) and coculture with bladder smooth muscle cells. The MDSC were isolated from gastrocnemius muscle biopsies of normal 3-6 week-old Sprague-Dawley rats and purified by the preplate technique. Cells that took approximately 6 days to adhere to the collagen-coated flasks were termed late preplate (LP) cells, and were used in all the experiments. The early plate (EP) cells (pp1-pp4) contained some myogenic cells but were mostly fibroblasts (< 15% desmin+ cells) whereas the LP cells (pp5-pp6) were highly purified muscle-derived cells (pp6) (> 90% desmin+ cells). The muscle-derived stem cells (LP cells) were CD34+ or Sca-1+, CD45- and desmin+ by immunohistochemical staining. After two days of co-culture with bladder smooth muscle cells, about 25% of the muscle-derived stem cells were positive for alpha-smooth muscle actin (alpha-SMA)+. RT-PCR for alpha-SMA was positive in the VEGF stimulated MDSC, but negative in the absence of VEGF. In conclusion, rat muscle-derived stem cells exhibited stem cell properties (CD34+ or Sca-1+), and were not of hematogeous (CD45-) but of myogenic origin (desmin+). RT-PCR of alpha-SMA was positive in the VEGF stimulated muscle-derived stem cells.
Use and Preference of Mass Media for Transferring Household Messages to Rural Families in India (1992)

This paper examines the importance of the roles played by radio, television and the newspapers in bring developmental messages to rural households and villages. It suggests that programmes and articles written to upgrade the skills and knowledge of the womenfolk in these villages should prove to be beneficial to the rural society as a whole as women play a significant role in the home as well as in operations outside the home.
Functions of Thiol-Disulfide Oxidoreductases in <i>E. coli</i> : Redox Myths, Realities, and Practicalities (2003)

A large family of enzymes contributes to the thiol-disulfide redox environment of the cells of most organisms. These proteins belong to pathways that carry out a variety of reactions, including the promotion of disulfide bond formation in extracytoplasmic proteins, the isomerization of proteins with incorrect disulfide bonds, and the reduction of disulfide bonds in the active sites of cytoplasmic proteins. Although the redox activities of these proteins measured in vitro often is consistent with the role (oxidant or reductant) these proteins perform in vivo, this is not always the case. The measured redox potentials can even suggest a function for a protein opposite of that which it carries out in the cell. Structural features of such proteins can contribute to a direction of electron transfer inconsistent with the redox potential. Furthermore, the environment in which such proteins are found may determine the protein's physiological role. Detailed analysis of these proteins in Escherichia coli provides strains that are useful for biotechnological purposes. Increasing the activity of certain of these proteins in the cell envelope or altering the thiol-disulfide redox environment of the cytoplasm to make it more oxidizing enhances the yield of useful disulfide bond-containing proteins such as tissue plasminogen activator and immunoglobulins.
Ion-Pair Chromatographic Separation of Water-Soluble Gold Monolayer-Protected Clusters (2006)

We demonstrate the efficacy of ion-pair chromatography for separations of samples of charged, polydisperse, water-soluble gold nanoparticles protected by monolayers of N-acetyl-l-cysteine and of tiopronin ligands. These nanoparticle mixtures have 1-2-nm-diameter Au core sizes as estimated from UV-visible spectra of the separated components. This size range encompasses the transition from bulk metal to molecular properties. The nanoparticle mixtures were resolved, the smallest nanoparticles eluting first, on an octadecylsilyl (C18) column using isocratic elution with a methanol/water mobile phase containing tetrabutylammonium fluoride (Bu4N+F-) and phosphate buffer. The column retention increases with Bu4N+F- concentration, lowered pH, and decreasing methanol volume fraction. The retention mechanism is dominated by ion-pairing in either the mobile phase or at the stationary/mobile-phase interface. Size exclusion effects, used in many previous nanoparticle separations, are insignificant.
Thermostabilization of cellulosomal endoglucanase EngB from <i>Clostridium cellulovorans</i> by <i>in vitro</i> DNA recombination with non‐cellulosomal endoglucanase EngD (2002)

Enhancement of enzyme thermostability by protein engineering gives us information about the thermostabilization mechanism as well as advantages for industrial use of enzymes. In this study, we enhanced the thermostability of endoglucanase EngB, one component of the cellulase complex (cellulosome) from Clostridium cellulovorans, by the directed evolution technique. The library was constructed by in vitro recombination of the genes for EngB and non-cellulosomal cellulase EngD, based on the fact that the catalytic domains of both cellulases were highly homologous. To obtain thermostable clones without loss of activity, the library was screened by a combination of activity and thermostability screening. We obtained three mutants out of 8000 selected clones that showed significantly higher thermostability than those of EngB and EngD without compromising their endoglucanase activities. One of the mutants possessed a sevenfold higher thermostability than EngB. The possible mechanisms of thermostabilization are discussed.
Development Of Hermetic Swing Compressors For CO2 Refrigerants (2002)

Due to global environmental concerns natural refrigerants are drawing more and more attention in the refrigeration and air conditioning industry. Among them, particularly CO2 is easy to apply to refrigeration system, because it has hardly any problems of toxicity and flammability and, in addition, is obtainable at low cost. Therefore, we have developed a compressor for CO2 heat pump water heater that can provide high temperature water at low running cost. From the points of physical properties, the density of CO2 is high and its refrigeration capacity per unit volume is large. It means that the displacement of CO2 compressors becomes small and, as a result, the influence of leakage on capacity becomes larger and the volumetric efficiency tends to be lower. In addition, its operating pressure is high and the differential pressure is large. Due to a large differential pressure, leakage increases and to maintain the reliability becomes difficult. Therefore, we focused a swing compressor that can maintain high efficiency and reliability even under a large differential pressure. For high efficiency, we designed the dimensions of the cylinder and the clearance of rotating parts to minimize the leakage gap. For high reliability, we carried out a stress analysis for the parts which receive a large differential pressure. The durability tests show that the adopted methods resulted in no problems around the sliding parts such as a bearing. As a result, we achieved a high seasonal COP of more than 3.0 by the CO2 heat pump water heater mounted with the compressor exclusively designed for CO2. INTRODUCTION In recent years the concern for natural environment such as ozone layer depletion and global warming is growing. Under these circumstances, natural refrigerants such as CO2, HC and ammonia are drawing attention as substitutes for HFCs. Among these refrigerants CO2 in particular has advantages of nontoxicity, nonflammability and low cost. Therefore, research and development (1), (2), (3) in CO2 as a refrigerant for air conditioning and heat pump systems is growing. Under these circumstances the residential CO2 heat pump water heater have been launched into the market since February 2002 by Daikin industries Ltd. This unit gives energy saving of more than 70% in comparison with the conventional electric water heater and, in addition, by utilization of midnight electricity rate the running cost is cut down by 20 to 25% based on the electricity rate system in Japan. We developed a swing compressor with high efficiency and reliability for CO2 heat pump water heaters. This paper explains the construction and characteristics of a swing compressor mounted on the CO2 heat pump water heater. COMPRESSOR TYPE Fig. 1 shows typical operating conditions with CO2 and R410A. As mentioned before, it is necessary to solve the technical problems such as a compressor for CO2 is leakable and its operating pressure difference is high. The following study explains how these problems are solved.
Lumpidin, a Novel Biomarker of Some Ochratoxin A Producing Penicillia (2001)

The novel compound lumpidin (1) has been isolated as a major compound from an isolate of Penicillium nordicum. Compound 1 is a diketopiperazine with a unique ring system likely to be a condensate of one mole each of L-tryptophan, L-phenylalanine, and L-homo-proline. The fact that 1 has been detected from only three out of sixteen isolates of P. nordicum indicates that lumpidin-producing isolates might represent a separate and third ochratoxin A producing Penicillium species.
Schmorl Nodes, Disc Degeneration, and Pain (2014)

Schmorl nodes (SNs) of the lumbar spine are an ill-defined entity sometimes referred to as intravertebral disc herniation and by others as end plate irregularities. They are often considered a radiographic curiosity with no clinical consequences. As part of a large-scale population-based study of the Hong Kong Degenerative Disc Disease cohort, sagittal T2-weighted magnetic resonance images of the lumbar spine were analyzed in 2,499 volunteers. SNs were found in 16.4% of the study population, being most common at L1/2 and L2/3 (54.1%). Multivariate logistic regression revealed that males, taller and heavier individuals had an increased likelihood of SN ( p < 0.005), suggesting mechanical factors may play a role in their pathogenesis. On the contrary, SNs were independent of age. Overall presence of SN was associated with disc degeneration ( p < 0.001), and linearly correlated ( R2 = 0.97) with increase in severity of degeneration. SN were particularly associated with severe disc degeneration at L1/2 and L2/3 with 22- to 15-fold increased odds, respectively ( p < 0.0001), but less than 5-fold increased odds ( p < 0.001) were noted in the lower lumbar spine. This presentation will discuss the various radiologic appearances of end plate irregularities that may be considered as SNs and propose a new classification based on morphology. In addition, morphological patterns tend to cluster in a particular manner to suggest different etiological factors may be associated with different subtypes of SNs. The relevance of end plate changes in the etiology of lumbar disc degeneration will also be discussed. Disclosure of Interest None declared
Role of MIP-2 in neutrophil migration and tissue injury in the herpes simplex virus-1-infected cornea. (1998)

Collectively, the results suggest that MIP-2 is the major chemokine that attracts neutrophils into the HSV-1 infected cornea, where the cells directly or indirectly cause tissue injury. Resident corneal cells and inflammatory cells contribute to MIP-2 synthesis, whereas KC production seems to be confined largely to corneal cells.
Thiophene-Containing Pechmann Dye Derivatives (2010)

Thiophene-containing Pechmann dyes (unsaturated exo-5,5-dilactones) were easily prepared by Cu-catalyzed dehydration of the corresponding β-aroylacrylic acids. Introduction of long alkyl chains greatly enhances solubility. Isomerization of an alkyl-substituted thiophene Pechmann dye (TPD) gave the corresponding endo-6,6-dilactone. The redox and electronic properties of these new dyes were investigated by CV, UV/vis, and fluorescence spectroscopy and DFT and TD-DFT computations. Strong absorption and emission in the visible region were recorded.
Evidence for structural relaxation in measurements of hydrogen diffusion in rf-sputtered boron-doped<i>a</i>-Si:H (1990)

H diffusion in boron-doped rf-sputter-deposited hydrogenated amorphous Si (a-Si:H) was measured by secondary ion mass spectrometry profiles of deuterium in annealed a-Si:H/a-Si:(H,D)/a-Si:H multilayers. The exponent \ensuremath{\alpha}=0.7\ifmmode\pm\else\textpm\fi{}0.1 of the diffusion constant D(t) =${\mathit{D}}_{00}$(\ensuremath{\omega}t${)}^{\mathrm{\ensuremath{-}}\mathrm{\ensuremath{\alpha}}}$ strongly decreases after annealing for \ensuremath{\sim}50 h at 180 \ifmmode^\circ\else\textdegree\fi{}C and \ensuremath{\sim}35 h at 225 \ifmmode^\circ\else\textdegree\fi{}C. It then increases to \ensuremath{\sim}0.8--1, i.e., the diffusion is nearly quenched, at longer annealing periods extending up to 1100 h. This behavior is believed to result from modifications in the microvoid system related to structural relaxation of the Si network.
Direction discriminating hearing aid system (1991)

A visual display was developed for people with substantial hearing loss in either one or both ears. The system consists of three discreet units; an eyeglass assembly for the visual display of the origin or direction of sounds; a stationary general purpose noise alarm; and a noise seeker wand.
Single Molecule Measurements of Repressor Protein 1D Diffusion on DNA (2006)

We used single-molecule imaging techniques and measured the one-dimensional diffusion of LacI repressor proteins along elongated DNA to address the long-standing puzzle of why some proteins find their targets faster than allowed by 3D diffusion. Our analysis of the LacI transcription factor's diffusion yielded four main results: (1) LacI diffuses along nonspecific sequences of DNA in the form of 1D Brownian motion; (2) the observed 1D diffusion coefficients ${D}_{1}$ vary over an unexpectedly large range, from $2.3\ifmmode\times\else\texttimes\fi{}{10}^{\ensuremath{-}12}\text{ }\text{ }{\mathrm{cm}}^{2}/\mathrm{s}$ to $1.3\ifmmode\times\else\texttimes\fi{}{10}^{\ensuremath{-}9}\text{ }\text{ }{\mathrm{cm}}^{2}/\mathrm{s}$; (3) the lengths of DNA covered by these 1D diffusions vary from 120 nm to 2920 nm; and (4) the mean values of ${D}_{1}$ and the diffusional lengths indeed predict a LacI target binding rate 90 times faster than the 3D diffusion limit.
Schedules for hepatitis B vaccination of risk groups: balancing immunogenicity and compliance (2007)

For rapid seroconversion and almost immediate protection in the short term, a (super)accelerated schedule could be used in at-risk groups. As long-term protection data with these (super) accelerated schedules have not been documented yet, a fourth dose at month 12 is still required. A shortened schedule (0.1.4 months) might be an alternative worth considering compared with the standard 0.1.6, as it convenes to internationally accepted minimum dose intervals and offers earlier protection. There is a clear need to study the long-term protection and effectiveness of the primary part of (super)accelerated schedules.
Immune control of herpesvirus latency (1979)

Introduction 267 Mechanisni of 1,atcncy 268 Imniunological Control of Virus Spread and Development of Clinical I>ise:~se 268 Role of the Inimune Response in Preventing the Establishlnent of I*;~tency and M;~intailiing the Virus in a Latent State 272
Simulation of the Forage Harvest Cycle for Asset Allocation (2018)

Abstract. Harvesting corn () for silage requires the coordination of multiple pieces of equipment to ensure rapid and economical production of silage. A model of corn harvest for silage production, capable of predicting machine work states and total harvest time for an entire field, using a single harvester, and any number of specific transport vehicles, as a function of machine specifications and field properties was developed. Three forage harvesting systems were observed using Global Positioning System (GPS) and the collected data used for model validation. The harvest model predicted harvest times within 10% of observed data and yielded similar results to a previously published cycle analysis. Model scenarios were used to explore the effect of differently sized transport vehicles on harvest time and it was found that placing transport vehicles with longer cycle times at the end of the rotation has the potential to reduce harvest time. This model can be used to determine the optimal number of transport vehicles and their dispatch order to reduce total harvest time. Keywords: Cycle analysis, Forage harvesting, Harvesting, Machinery selection, Silage.