gebhart/patrepeval huggingface dataset:
Fluorescently labelled amino acids and peptides for efficient screening <i>in situ</i> and <i>in vivo</i> (2015)

A variety of amino acids with fluorescent side groups (Faa's) are now commercially available and can be inserted into peptides through solid-phase synthesis. Concentration of single Faa can be quantified in a mixture of multiple Faa's without isolation, by using 2-dimensional fluorescence spectroscopy combined with the least-squares analysis. Peptide libraries that consist of 8 sub-libraries, each being labelled with different Faa were synthesized. The multiply labelled library was employed to find cancer-cell binding peptides, by quantifying sub-libraries that were most abundantly found in targeting cells. The technique is applicable for efficient screening of peptides both in situ and in vivo.
<scp>EDIFICE</scp> Melanoma survey: knowledge and attitudes on melanoma prevention and diagnosis (2015)

Awareness of melanoma, early-diagnosis procedures and preventive behaviour has improved in the general French population since 1990. However, despite the good level of information, numerous misconceptions persist. Improved information campaigns in the future may help reduce the ever-increasing incidence of melanoma in France.
A wavelet based method for the estimation of the power spectrum from irregularly sampled data (1998)

The problem of estimating the power spectrum from irregularly sampled data appears in many technical applications. Recently it has been studied by many authors. For the current status of the problem see for instance Benedict et.al. (1998) and Ware (1998) and references therein. Most techniques for spectrum estimation can be classified into one of the following groups: slotting technique and cosine transform, direct transformation and reconstruction with uniform resampling plus FFT. In this paper we present a method of the third group above. We recontruct a signal using a wavelet approximation, resample the approximated signal uniformly and finally compute the power spectrum with the standard FFT. The figure below gives an example of an irregularly sampled data and its wavelet approximation. 0.44 0.441 0.442 0.443 0.444 0.445 0.446 0.447 0.448 0.449 0.45 -1 0 1 2 3 4 5 6 7 A signal with a wavelet approximation
Über die Ringisomerisierung von Isoflavonen, XIII. Endgültige Konstitutionsaufklärung und vollständige Synthese des Iridins (1966)

Abstract Durch die Synthese von 5.6.3′.4′.5′‐Pentamethoxy‐7‐äthoxy‐isoflavon und die Verknüpfung von Irigenin mit α‐Acetobromglucose wurde für das natürlich vorkommende Iridin aus Iris florentina die Konstitution eines 5.7.3′‐Trihydroxy‐6.4′.5′‐trimethoxy‐isoflavon‐7‐mono‐β‐D‐glucosids endgültig bewiesen.
Prolonged Paralysis With Atracurium Infusion (1995)

Haenel, James B. RRT; Moore, Frederick A. MD FCCM; Moore, Ernest E. MD FCCM; Burch, Jon M. MD Author Information
CONSTANT ABSOLUTE ETHANOL INTAKE BY SARDINIAN ALCOHOL-PREFERRING RATS INDEPENDENT OF ETHANOL CONCENTRATIONS (1997)

The present study was designed to evaluate ethanol drinking behaviour in Sardinian alcohol-preferring (sP) and Sardinian alcohol-non-preferring (sNP) rats in the presence of different ethanol concentrations. Ethanol intake was tested under the two-bottle, free-choice regimen and continuous access schedule. Ethanol-naive sP and sNP rats were initially given ethanol solution at the standard, constant concentration of 10% (v/v) for 8 consecutive days (Phase 1). As expected, daily ethanol intake in sP rats rose from 4 to approximately 6 g/kg; in contrast sNP rats consumed < 10 g/kg/day ethanol. Subsequently, an ascending series of ethanol concentrations, ranging from 3 to 60% (v/v), was presented to sP and sNP rats over a 28-day period (Phase 2). At concentrations varying from 7 to 30%, sP rats consumed constant amounts of absolute ethanol per kg of body weight (approximately 6.0 g/kg/day). Daily ethanol intake in sNP rats remained constantly lower than 1.0 g/kg, irrespective of the ethanol concentration. Data from Phase 2 demonstrate the ability of sP rats to precisely adjust daily ethanol intake and support the hypothesis that voluntary ethanol drinking in sP rats is sustained by specific pharmacological effects of ethanol.
DNA hybridization detection by porous silicon-based DNA microarray in conjugation with infrared microspectroscopy (2007)

A method is described for the label-free detection of DNA hybridization on porous silicon (por-Si), based upon the pairing of oligonucleotide chemistry and standard silicon nanotechnology. Por-Si with a pore diameter of approximately 30 nm was used to immobilize probe DNA. Infrared microspectroscopy was employed to monitor the hybridization of probe-DNA immobilized on pore surfaces with its complementary DNA (target-DNA). The immobilization of probe DNA on por-Si facilitates hybridization detection for a small sensing area (approximately 50×50 μm2) with a high detection efficiency. In this study, we fabricated a porous silicon-based DNA microarray (por-Si-microarray) using photolithographic and Si anodizing techniques. We demonstrate that DNA hybridization can be detected on a por-Si-microarray through the analysis of infrared absorption spectral profiles in the region where the vibration modes of the bases appear. This present approach demonstrates that por-Si-microarray in conjugation with infrared microspectroscopy has potential application in DNA sensing chips.
Adsorption of H<sub>2</sub>O, CO<sub>2</sub> and Xe on Soft Surfaces (2008)

The interactions of water, carbon dioxide, and Xe with octadecanethiol (C(18)H(37)SH, ODT) self-assembled monolayers (SAMs) were studied under ultrahigh vacuum conditions employing temperature-programmed desorption and optical diffraction measurements. The ODT layer was grown on a 1 nm thick gold film deposited over a Ru(001) single-crystal substrate. The gases used in this report differ in their lateral interactions while adsorbed on ODT-SAM being either repulsive (Xe) or attractive (H(2)O, CO(2)). The activation energies for desorption of the first layer from ODT are E(a) = 3.6 +/- 0.9, 4.1 +/- 0.5, and 8.5 +/- 0.9 kcal/mol for Xe, CO(2), and H(2)O, respectively. Sticking probabilities of the three gases on the soft ODT surface are S(0) = 0.7 +/- 0.1, 0.8 +/- 0.1, and 0.95 +/- 0.05 for xenon, CO(2), and water, respectively, derived from the respective adsorption curves. Optical diffraction studies from multilayer coverage grating of Xe on ODT-SAM have demonstrated that sublimation is a thermodynamically more favorable process over diffusion and wetting. The significantly lower binding energy of the first layers of H(2)O and CO(2) adsorbed on the soft surface of ODT compared to that on clean metals and oxides, reflects generally weak (CO(2)) and hydrophobic (H(2)O) interactions that are important for understanding the behavior of these molecules on interfaces that are found in biological systems.
Analysis of Molybdenum in Biological Materials (1979)

A procedure for the determination of molybdenum in serum, red blood cells, and urine is described. The low concentration of molybdenum in most unexposed individuals requires the sensitivity obtained using atomic absorption spectrophotometry and electrothermal atomization. Spike recovery tests indicate that low temperature ashing is required for accuracy. Severe matrix interferences preclude wet ashing or high-temperature ashing as sample pretreatments. Using the method described, it is possible to distinguish between industrially exposed and unexposed individuals.
Über Aminosäuren und Peptide, V. Syntheseversuche in der Reihe der 3.6‐Epidithio‐2.5‐dioxo‐piperazin‐Antibiotika Gliotoxin, Sporidesmin, Aranotin, Chaetocin und Verticillin, V<sup>1)</sup> Synthese des Epitrithio‐ und Epitetrathio‐L‐prolyl‐L‐prolin‐anhydrids (1972)

Abstract Reines und kristallines cis ‐3.6‐Dimercapto‐L‐prolyl‐L‐prolinanhydrid ( 5 ) ließ sich durch Boranatreduktion aus 3.6‐Epidithio‐L‐prolyl‐L‐prolinanhydrid ( 2 ) gewinnen. Mit SCl 2 und S 2 Cl 2 bildete das Dimercaptan Epitrithio‐ ( 6 ) bzw. Epitetrathio‐L‐prolyl‐L‐prolinanhydrid ( 4 ).
Three terminal YBaCuO Josephson device with quasi-particle injection gate (1989)

A novel type of three-terminal Josephson device called the SCST (superconducting current switching transistor), was fabricated using high-T/sub c/ LnBaCuO (Ln=Y, Er) thin epitaxial and/or polycrystalline films. The hot quasi-particle injection effect on the Josephson (or superconducting) current in the films was closely examined. The zero bias drain current was efficiently suppressed by the injection of the hot quasi-particles through the gate electrode. A comparison of the experimental results and analyses based on the BCS theory suggests that the main mechanism of the current modulation is the nonequilibrium superconductivity due to the accumulation of the excess quasi-particles. The maximum current modulation gain was 5 approximately 7 and the modulation current ranged as low as 3 approximately 10 A/cm/sup 2/.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Reverse micelles in protein separation: The use of silica for the back‐transfer process (1993)

In order to use reverse micellar solutions successfully for the separation of proteins, good methods are needed to recover the biomolecules into an aqueous environment after solubilization into organic micellar media. Usually the recovery is accomplished by equilibrating the protein-loaded reverse micellar solution with a water phase containing an appropriate salt (back-transfer). In this article we describe an alternative "back extraction" procedure which is based on the addition of silica to the protein-containing reverse micellar solution. In this way, the water is stripped from the reverse micellar solution. [i.e., bis(2-ethylhexyl) sodium sulfosuccinate (AOT)/isooctane/water] and the proteins adsorb to the silica particles. The adsorption process is shown to be practically quantitative. The subsequent recovery of the proteins form the silica into an aqueous solution turns out to be most efficient at alkaline pH (pH 8); 60-80 of the total protein (alpha-chymotrypsin or trypsin) could be recovered. The specific enzyme activity at the end of the whole cycle can be as high as 80-100%. The procedure is applied also for the back extraction from micellar solutions in which, instead of AOT, a biocompatible surfactant such as a synthetic short-chain lecithin was used. It is shown that the recovery of a alpha-chymotrypsin and trypsin is also achievable under these conditions in quite good yield and under good maintenance of the enzyme's catalytic activity.
Clinically Significant Blunt Cardiac Trauma: Role of Serum Troponin Levels Combined with Electrocardiographic Findings (2001)

The combination of ECG and cTnI identifies reliably the presence or absence of Sig-BCT. Patients with an abnormal ECG and cTnI need close monitoring for at least 24 hours. Patients with a normal admission ECG and cTnI can be safely discharged in the absence of other injuries.
Restraint Stress Alters Nociceptin/Orphanin FQ and CRF Systems in the Rat Central Amygdala: Significance for Anxiety-Like Behaviors (2014)

Corticotropin releasing factor (CRF) is the primary mediator of stress responses, and nociceptin/orphanin FQ (N/OFQ) plays an important role in the modulation of these stress responses. Thus, in this multidisciplinary study, we explored the relationship between the N/OFQ and the CRF systems in response to stress. Using in situ hybridization (ISH), we assessed the effect of body restraint stress on the gene expression of CRF and N/OFQ-related genes in various subdivisions of the amygdala, a critical brain structure involved in the modulation of stress response and anxiety-like behaviors. We found a selective upregulation of the NOP and downregulation of the CRF1 receptor transcripts in the CeA and in the BLA after body restraint. Thus, we performed intracellular electrophysiological recordings of GABAA-mediated IPSPs in the central nucleus of the amygdala (CeA) to explore functional interactions between CRF and N/OFQ systems in this brain region. Acute application of CRF significantly increased IPSPs in the CeA, and this enhancement was blocked by N/OFQ. Importantly, in stress-restraint rats, baseline CeA GABAergic responses were elevated and N/OFQ exerted a larger inhibition of IPSPs compared with unrestraint rats. The NOP antagonist [Nphe1]-nociceptin(1-13)NH2 increased the IPSP amplitudes in restraint rats but not in unrestraint rats, suggesting a functional recruitment of the N/OFQ system after acute stress. Finally, we evaluated the anxiety-like response in rats subjected to restraint stress and nonrestraint rats after N/OFQ microinjection into the CeA. Intra-CeA injections of N/OFQ significantly and selectively reduced anxiety-like behavior in restraint rats in the elevated plus maze. These combined results demonstrate that acute stress increases N/OFQ systems in the CeA and that N/OFQ has antistress properties.
Rapidly reconfigurable all-optical universal logic gates (2006)

We present designs and simulations for a highly cascadable, rapidly reconfigurable, all-optical, universal logic gate. We will discuss the gate's expected performance, e.g. speed, fanout, and contrast ratio, as a function of the device layout and biasing conditions. The gate is a three terminal on-chip device that consists of: (1) the input optical port, (2) the gate selection port, and (3) the output optical port. The device can be built monolithically using a standard multiple quantum well graded index separate confinement heterostructure laser configuration. The gate can be rapidly and repeatedly reprogrammed to perform any of the basic digital logic operations by using an appropriate analog electrical or optical signal at the gate selection port. Specifically, the same gate can be selected to execute one of the 2 basic unary operations (NOT or COPY), or one of the 6 binary operations (OR, XOR, AND, NOR, XNOR, or NAND), or one of the many logic operations involving more than two inputs. The speed of the gate for logic operations as well as for reprogramming the function of the gate is primarily limited to the small signal modulation speed of a laser, which can be on the order of tens of GHz. The reprogrammable nature of the universal gate offers maximum flexibility and interchangeability for the end user since the entire application of a photonic integrated circuit built from cascaded universal logic gates can be changed simply by adjusting the gate selection port signals.
Toward the Characterization of Peptidoglycan Structure and Protein−Peptidoglycan Interactions by Solid-State NMR Spectroscopy (2008)

Solid-state NMR spectroscopy is applied to intact peptidoglycan sacculi of the Gram-negative bacterium Escherichia coli. High-quality solid-state NMR spectra allow atom-resolved investigation of the peptidoglycan structure and dynamics as well as the study of protein-peptidoglycan interactions.
Optical properties and applications of diamond and diamondlike carbon thin films (1990)

Pure diamond has excellent mechanical and infra red properties and would make an
ideal window material were it readily available. Synthetic diamond films can be
grown with good infra red transmission and used as protective coatings. Many
coating requirements can already be met using diamond-like carbon films.
Trimethylamine Borane: A New Single-Source Precursor for Monolayer h-BN Single Crystals and h-BCN Thin Films (2016)

Due to their exceptional chemical and thermal stabilities as well as electrically insulating property, atomically thin hexagonal boron nitride (h-BN) films have been identified as a promising class of dielectric substrate and encapsulation material for high-performance two-dimensional (2D) heterostructure devices. Herein, we report a facile chemical vapor deposition synthesis of large-area atomically thin h-BN including monolayer single crystals and C-doped h-BN (h-BCN) films utilizing a relatively low-cost, commercially available trimethylamine borane (TMAB) as a single-source precursor. Importantly, pristine 2D h-BN films with a wide band gap of ∼6.1 eV can be achieved by limiting the sublimation temperature of TMAB at 40 °C, while C dopants are introduced to the h-BN films when the sublimation temperature is further increased. The h-BCN thin films displayed band gap narrowing effects as identified by an additional shoulder at 205 nm observed in their absorbance spectra. Presence of N–C bonds in the h-BCN structures with a doping concentration of ∼2 to 5% is confirmed by X-ray photoelectron spectroscopy. The inclusion of low C doping in the h-BN films is expected to result in constructive enhancement to its mechanical properties without significant alteration to its electrically insulating nature. This study provides new insights into the design and fabrication of large-area atomically thin h-BN/h-BCN films toward practical applications and suggests that the range of precursors can be potentially extended to other anime borane complexes as well.
Freecyto: Quantized Flow Cytometry Analysis for the Web (2020)

Flow cytometry (FCM) is an analytic technique that is capable of detecting and recording the emission of fluorescence and light scattering of cells or particles (that are collectively called "events") in a population. A typical FCM experiment can produce a large array of data making the analysis computationally intensive. Current FCM data analysis platforms (FlowJo, etc.), while very useful, do not allow interactive data processing online due to the data size limitations. Here we report a more effective way to analyze FCM data. Freecyto is a free, easy-to-learn, Python-flask-based web application that uses a weighted k-means clustering algorithm to facilitate the interactive analysis of flow cytometry data. A key limitation of web browsers is their inability to interactively display large amounts of data. Freecyto addresses this bottleneck through the use of the k-means algorithm to quantize the data, allowing the user to access a representative set of data points for interactive visualization of complex datasets. Moreover, Freecyto enables the interactive analyses of large complex datasets while preserving the standard FCM visualization features, such as the generation of scatterplots (dotplots), histograms, heatmaps, boxplots, as well as a SQL-based sub-population gating feature. We also show that Freecyto can be applied to the analysis of various experimental setups that frequently require the use of FCM. Finally, we demonstrate that the data accuracy is preserved when Freecyto is compared to conventional FCM software.
The effect of crosslinking on mechanical properties of ldpe filled with organic fillers (1998)

Abstract Mechanical properties of low density polyethylene filled with various organic fillers were investigated. Different effect of different fillers on the properties was observed and the effect of crosslinking of these materials is also different. Fine anisotropic fillers behave similarly as inorganic fillers. The effect of crosslinking is the highest for composites containing large particulate fillers like beech wood flour. The effects are discussed in terms of mechanical behaviour and crosslinking degree determined from extraction or equilibrium swelling data. A formation of covalent bonds between the filler surface and polymeric matrix is proposed as a result of crosslinking.
Personal autonomy and self‐determination are crucial for professionalism in healthcare (2023)

In our role as medical educators and researchers, we support in the strongest manner possible the personal autonomy and self-determination of our students, patients, and colleagues. A fundamental value of the medical profession is for the patient to have personal autonomy in their healthcare decisions, including how they would like to be identified. It is, and will continue to be, an important priority to be taught and encouraged throughout healthcare education,1 and it begins in the anatomy laboratory with the proper and respectful treatment of body donors.2 Learning this value continues with respecting and accepting this same autonomy in fellow students, staff, faculty, and patients. There are some who question whether foregrounding the value of personal autonomy and the right to self-identification in health professions education is an appropriate use of resources and teaching time in an already crowded curriculum. Let us be clear: understanding this value and addressing it in the correct language3 are of paramount importance to the well-being of all involved in healthcare and should be a recurring theme throughout their education and career.4 Personal autonomy is one of the four pillars of bioethics and is a strong antidote to the paternalism and mistakes of recent eras of healthcare delivery. Any individual should be able to make their personal identity known without fear or ridicule, and this is especially important in healthcare fields. Time and resources should be devoted to educating our future healthcare professionals in the proper way to address patients, students, and colleagues. This should occur during all phases of healthcare education and should be practiced and exemplified by those who teach in healthcare. Appropriate identification of individuals, and their right to choose how they wish to be identified, is a fundamental tenet of healthcare professionalism and demands recognition as an integral principle of human rights. Complete healthcare starts with a thorough understanding of the patient, and that includes respect of personal autonomy. Acquiring skills and knowledge around the appropriate application of such principles is necessary as one of many essential components of medical education and training.
DYNAMIC ROOT RESPONSES TO WATER DEFICITS (1992)

Water absorption is influenced by the geometric distribution of viable roots and the availability of water at root surfaces. Utilization of additional plant energy for the extensive and localized development of root systems is a high risk investment of carbon by the plant, especially during short-term water deficits. Nonuniform root distribution, resulting from root growth within networks of continuous soil macropores, reduces the efficiency of water absorption by minimizing contact between roots and increasing the geometric mean pathway of water from the bulk soil to the root. Root clustering in these soil niches causes greater intraspecific competition for the limited water, especially as soil water deficits develop more deeply within the soil profile. Current methods for quantifying and predicting plant root and soil water relationships are reviewed, and some research and modeling imperatives are presented.
Red Blood Cell Indices, Cation Content, and Membrane Cation Transports (1998)

In sickle cell disease, in the homozygous state, the increased heterogeneity of erythrocytes results mainly from membrane defects secondary to Hb S polymerization and the increased survival of F cells. The density distribution curve, using phthalate esters or the red blood cell indices measured with the H*3 system, are useful methods for the hematological follow-up of patients under specific therapies. The methods evaluating the red blood cell cation contents and the abnormal membrane potassium transport pathways are also described, in order to evaluate agents which can restore normal hemoglobin concentration and water content in dehydrated sickle cells.
1,1-Bis(3'-indolyl)-1-(p-biphenyl)methane inhibits basal-like breast cancer growth in athymic nude mice (2007)

CDIM9 showed potent antiproliferative effects on basal-like breast cancer cell in tissue culture and dramatic growth inhibition in animal models at safe doses. These findings justify further development of this drug for treatment of basal-like breast cancer.