gebhart/patrepeval huggingface dataset:
Assembly and calcium-induced cooperativity of Limulus IV hemocyanin: a model system for analysis of structure-function relationships in the absence of subunit heterogeneity (1983)

Hemocyanins, the high molecular weight copper proteins which serve as oxygen carriers in many arthropods and molluscs, are representative of multisubunit complexes which are capable of reversible dissociation and assembly. Although reversible, in many hemocyanins these processes are not in true thermodynamic equilibria, and it has been suggested that there is "microheterogeneity" among the molecules in solution. An alternative explanation is that their complex behavior is due to the existence of quaternary interactions between structurally distinct types of subunits within the native molecule which have varying pH and ionic strength sensitivity. Limulus IV hemocyanin was used as a model system to examine structure-function relationships in the absence of subunit heterogeneity. Purified subunit IV of Limulus polyphemus hemocyanin is homogeneous by a number of electrophoretic and immunological criteria and is capable of undergoing pH-dependent self-assembly into hexamers. The monomer-hexamer transition was found to be an equilibrium whose rate is dependent on the presence or absence of calcium ions. The observation that the assembly of this homopolymer behaves as a true equilibrium suggests that the nonequilibrium dissociation profiles observed for native Limulus hemocyanin are related to the extensive subunit heterogeneity of the native protein. In calcium-containing buffers, the monomer-hexamer transitions of Limulus IV hemocyanin can be described by a cooperative mechanism with approximately six protons per hexamer lost on assembly from acid pH and three protons gained on assembly from alkaline pH. Increased ionic strength or increased temperature favors dissociation. Like the native molecule, Limulus IV hemocyanin behaves as an allosteric protein.(ABSTRACT TRUNCATED AT 250 WORDS)
Metabolic Syndrome, C-Reactive Protein, and Mortality in U.S. Blacks and Whites: The Reasons for Geographic and Racial Differences in Stroke (REGARDS) Study (2014)

By definition, CRPMetS identifies more people at risk than MetS but still maintains a similar mortality risk. Incorporating CRP into the definition for MetS may be useful in identifying additional high-risk populations to target for prevention.
Comparison of clear-sky surface radiative fluxes simulated with radiative transfer models (2004)

The surface fluxes of several important radiatively active gases, including H2O, CO2, CH4, N2O, O3, and the chlorofluorocarbons CFC11 and CFC12, were simulated with the radiation band models from the National Center for Atmospheric Research (NCAR) community climate model 3 (CCM3), the single-column community atmospheric model (SCAM), and the Canadian global climate model 3 (GCM3). These results were compared with the measured fluxes for a very cold winter day and with the simulated results for other standard atmospheres using the line-by-line radiative transfer model (LBLRTM). The comparison shows that the total surface radiative flux contributed by all the greenhouse gases combined is well simulated by the SCAM and GCM3 radiation band models. The two models generally agree within about 1% of the line-by-line result for all the atmospheric conditions studied. The error in the total flux simulated by the older CCM3 code, however, can be as large as 7% depending on the atmospheric conditions. The SCAM code consistently models H2O better than the CCM3 and GCM3 codes, typically displaying errors of less than 1 W/m2 for all atmospheric conditions. All of the models have difficulty in modelling accurately the radiative flux of CH4 and N2O. In general, the inaccuracy increases, by as much as 200% in some cases, as the amount of H2O in the atmosphere increases. The source of the problem appears to be related to the overlapping bands of other gases. The error in the ozone flux varies from 5% to 15% for the CCM3 and SCAM models, and it can be as large as 30% for the GCM3 code. The CCM3 and SCAM models simulated the chlorofluorocarbon fluxes to within 0.06 W/m2, but this leads to relative errors of 20%–40% for the various atmospheric scenarios. The errors for the CFCs are even larger in the case of the GCM3 model.
Studies on fused pyrimidine derivatives. Part 15. Features and mechanistic considerations of the reaction of 5-(alkylaminomethylene)-6-methyleneperhydropyrimidine-2,4-diones with tropone (1995)

The reaction of 5-(alkylaminomethylene)-6-methyleneperhydropyrimidine-2,4-dione intermediates 3 with tropone 4 gave 2,5-ethanopyrido[2,3-ƒ]quinazolines 5 and/or 6,10a-methanopyrimido[4′,5′:4,5]cyclohepta[1,2-b]azocines 6. The formation of endo- and exo-[4 + 2] cycloadducts and their transformations into these products 5 and 6 will be discussed using the results of PM3 calculations. The X-ray structure of 1-benzyl-7,9-dimethyl-1,2,r-4a,c-5,6,7,8,9,10,c-10b-decahydro-2,5-ethanopyrido[2,3-ƒ]quinazoline-8,10,11-trione and 5-benzyl-1,3-dimethyl-1,2,3,4,r-4b,5,t-6,7,10,11-decahydro-6,10a-methanopyrimido[4′,5′:4,5]cyclopenta[1,2-b]azocine-2,4,10-trione are described.
Noonan syndrome type I with <i>PTPN11</i> 3 bp deletion: Structure–function implications (2004)

Noonan syndrome was recently reported to be caused by mutations in the PTPN11 gene in 40% of the cases. This gene encodes the nonreceptor-type protein tyrosine phosphatase SHP-2 and has been shown to be self down-regulated with the concurrency of two SH2 domains. Insertion of a specific loop (D'EF) from N-terminal SH2 domain into the SHP-2 active-site is responsible for the reversible inhibition of the phosphatase activity. Here we report the first in frame trinucleotide deletion resulting in the removal of Aspartate 61 (D61del), a key residue of the N-terminal SH2 D'EF loop. Energetic-based structural analysis and electrostatic calculations carried out on the wild-type and mutant proteins predict lower stability of the D'EF loop for the D61del variant as compared to the wild type indicating better access to the active site and most likely an enzyme activated for longer extent. Similar computations were performed on the previously functionally characterized gain-of-function D61Y mutant and similar behaviors were observed. The simulation data for the D61del and D61Y mutants suggest that both variants could yield more catalytic cycles than the wild-type molecule in the same timespan because of the opening of the active site. It also supports the notion that D61 plays a major role for proper down-regulation of the protein tyrosine phosphatase activity of SHP-2.
Long-term exposure to very low doses of bisphenol S affects female reproduction (2018)

Bisphenols belong to the endocrine disruptors, affecting reproduction even in extremely low doses. Bisphenol S (BPS) has become widely used as a substitute for the earlier-used bisphenol A; however, its harmlessness is questionable. The aim of this study was to evaluate the effect of BPS on folliculogenesis and oocyte quality after <i>in vivo</i> exposure to low doses of BPS. Four-week-old ICR females (<i>n</i> = 16 in each experimental group) were exposed to vehicle control (VC), BPS1 (0.001 ng BPS.g/bw/day), BPS2 (0.1 ng.g/bw/day), BPS3 (10 ng.g/bw/day) and BPS4 (100 ng.g/bw/day) for 4 weeks. Ovaries were subjected to stereology and nano liquid chromatography-mass spectrometry (LC/MS). Simultaneously, metaphase II oocytes were obtained after pregnant mare serum gonadotrophin and human chorionic gonadotrophin administration, followed by immunostaining. In particular, mating and two-cell embryo flushing were performed. We observed that BPS decreases the amount of ovarian follicles and BPS2 (0.1 ng.g/bw/day) affects the volume of antral follicles. Accordingly, ovarian proteome is affected after BPS2 treatment. While BPS2 dosing results mainly in cytoskeletal damage in matured oocytes, the effects of BPS3 and BPS4 seem to be due instead to epigenetic alterations in oocytes. Arguably, these changes lead to observed affection of <i>in vivo</i> fertilization rate after BPS3 and BPS4 treatment. BPS significantly affects female reproduction astoundingly in extremely low doses. These findings underline the necessity to assess the risk of ongoing BPS exposure for public health.
Simulation-Based Development of an Energy Management System for an Innovative Power-Splitting Hybrid Drive Train with a Double Rotating Electric Machine (2015)

This paper results from a governmental founded research project which includes the development, demonstration and evaluation of an innovative power-splitting hybrid drive train. The rear axle is driven electrically; the front axle is driven by a combustion engine (ICE) via a double rotating electric machine which enables a power-splitting operation of the presented hybrid drive train. An Energy-Management-System, which controls the components and contains a load strategy for the ICE, was simulation-based developed and optimized for the most efficient operation of the total system. Comparative simulations of alternative powertrains were analyzed to show the consumption advantage of the presented technology.
Variations in the essential oil of artemisia judaica L. Chemotypes related to phenological and environmental factors (1992)

Abstract The essential oil content and compositon of Artemisia judaica L. from the Sinai Peninsula (Egypt) and the Negev Desert (southern Israel) are related to a variety of factors such as season, plant age, and different plant parts. Differences were found also from samples collected at various locations. The contents fluctuated with the season, reaching the highest level during the late summer. Seasonal variations in the essential oil content occur one month earlier in Sinai plants than in Negev plants. The essential oil content in the leaves is higher than in the branches and flowers. No differences were found in the relative percentage of the components in the essential oil from different plant parts. Essential oil of A. judaica plants from the two regions was of two distinct chemotypes. An artemisyl‐oil type was predominant in the Negev samples, while a piperitone‐oil type was predominant in those collected in southern Sinai. These differences were evident in samples collected in the wild in all seasons, over a period of two years. Seedlings grown in a controlled environment, from seeds collected in the two regions, exhibited the same differences. It is, therefore concluded that these differences reflect genetic differences between the two populations.
Study on erosion resistance characteristics of Fe-MWCNT composite plating with respect to lead-free solder (2012)

Sn-3mass%Ag-0.5mass%Cu lead-free solder causes strong erosion of Fe plating, which is used in hand soldering. In order to improve the durability of the soldering iron tip, we tried Fe-MWCNT (Multi-walled Carbon Nanotube) composite plating on the tip. Microstructures and the erosion resistance of the Fe-MWCNT composite plating under heating were examined. On the basis of the SEM/EDS analysis, it was confirmed that there were MWCNTs in the Fe-MWCNT composite plating. Even when heated to 673 K, MWCNTs were found to be present in the plating film under a stable condition. It was shown that the performance of erosion resistance for lead-free solders under high temperature (673 K) is improved due to the MWCNT addition to the Fe plating.
Influence of cytosolic pH on receptor-mediated endocytosis of asialoorosomucoid (1988)

Inhibitors of specific steps in the endocytosis of galactose-terminating glycoproteins (asialoglycoproteins) by cultured rat hepatocytes have been described (J. Cell Biol. 98: 375-381, 1984). In particular, substitution of K+ for Na+ in the culture medium results in reduced delivery of ligand to lysosomes; ligand-receptor internalization, dissociation, and segregation remain normal. We have now demonstrated by direct microelectrode measurement that incubation of hepatocytes in K+-substituted medium results in a reduction of intracellular pH by greater than or equal to 0.5 U. In addition, we have shown that reduced intracellular pH in these cells produced by either direct (CO2 diffusion) or indirect (K+ substitution) acidification inhibits ligand delivery to lysosomes. Return of internalized ligand-receptor complex to the cell surface (diacytosis) is also inhibited by these manipulations. These studies suggest that intracellular pH may modulate specific steps involving vesicle translocation and fusion in the receptor-mediated endocytosis of asialoglycoproteins. Similar effects of direct acidification of hepatocytes by CO2 diffusion and indirect acidification by K+ substitution for Na+, on diacytosis and ligand delivery to lysosomes, suggest that K+ substitution may influence these events by altering intracellular pH.
Subdivision surface fitting for efficient compression and coding of 3D models (2005)

In this paper we present a new framework, based on subdivision surface fitting, for high rate compression and coding of 3D models. Our algorithm fits the input 3D model, represented by a polygonal mesh, with a piecewise smooth subdivision surface represented by a coarse control polyhedron. Our fitting scheme, particularly suited for meshes issued from mechanical or CAD parts, aims at getting close to the optimality in terms of control points number, while remaining independent of the connectivity of the input mesh. The found subdivision control polyhedron is much more compact than the original mesh and visually represents the same shape after several subdivision steps, without artifacts or cracks, like traditional lossy compression schemes. This control polyhedron is then encoded specifically to give the final compressed stream. Experiments conducted on several 3D models have proven the coherency and the efficiency of our framework, compared with existing compression methods.
A Novel Growth- and Cell Cycle-Regulated Protein, ASK, Activates Human Cdc7-Related Kinase and Is Essential for G<sub>1</sub>/S Transition in Mammalian Cells (1999)

A novel human protein, ASK (activator of S phase kinase), was identified on the basis of its ability to bind to human Cdc7-related kinase (huCdc7). ASK forms an active kinase complex with huCdc7 that is capable of phosphorylating MCM2 protein. ASK appears to be the major activator of huCdc7, since immunodepletion of ASK protein from the extract is accompanied by the loss of huCdc7-dependent kinase activity. Expression of ASK is regulated by growth factor stimulation, and levels oscillate through the cell cycle, reaching a peak during S phase. Concomitantly, the huCdc7-dependent kinase activity significantly increases when cells are in S phase. Furthermore, we have demonstrated that ASK serves an essential function for entry into S phase by showing that microinjection of ASK-specific antibodies into mammalian cells inhibited DNA replication. Our data show that ASK is a novel cyclin-like regulatory subunit of the huCdc7 kinase complex and that it plays a pivotal role in G1/S transition in mammalian cells.
Dynamic NMR study of tri-tert-butylcarbinol and of a carbinol-methylaluminoxane mixture (2000)

The reaction of tri-tert-butylcarbinol and oligomeric methylaluminoxane (MAO) prepared in toluene-d8 was studied by analysing the reaction mixture utilizing ROESY, HSQC and HMBC 2D NMR experiments. The formation of one main alkoxide was observed in the reaction. Dynamic 13C NMR and semiempirical PM3 molecular modelling were used in concert to study the tert-butyl group rotation barriers of this alkoxide. From several modelled acyclic and cyclic MAO fragments, an open chain—Al(Me)OAl(Me)—fragment was observed to be consistent with the NMR results. Copyright © 2000 John Wiley & Sons, Ltd.
<i>Staphylococcus aureus</i> endocarditis: distinct mechanisms of bacterial adhesion to damaged and inflamed heart valves (2019)

Cardiac valve damage and inflammation predispose to S. aureus endocarditis via distinct mechanisms. These findings may have important implications for the development of new preventive strategies, as some interventions might be effective in one risk state, but not in the other.
Highly sensitive humidity sensor for dielectric measurement (water absorption) in glass-fiber resins (2002)

The humidity sensor using the sensitive capacitive-dependent crystal is described. Presented is the probe sensitivity (dependence of df on the dCx). In addition, the new idea of excitation of the entire humidity sensor with stochastic test signals is described, and the humidity surface-measuring method is given. It includes the influence of test signals on the weighting function uncertainty and on the A/D-D/A conversion. The results of the air-humidity measurement and water absorption measurement in glass-fiber resins are shown experimentally. The uncertainty of the surface humidity measurement is less than 0.2% (T=15-25/spl deg/C and air-humidity=50 to 98%).
A Study on the Laser Conducting Ultrasonic Method for Non-Destructive Evaluation of Welding Part (2005)

This DOI is not currently attached to any metadata records. DOIs can’t actually ever be deleted (they’re persistent), but sometimes our members create DOIs in error. We do have a process to approximate deletion which we follow only in rare cases where the DOI has been genuinely created in error, and most crucially, if the DOI has never been published anywhere online or in print and never otherwise distributed to or communicated with anyone (authors, readers, reviewers, etc.
On the Laminar Flow Heat-Transfer of the Two-Dimensional Body Surface of Arbitrary Temperature Distribution (1954)

This report is concerned with the heat transfer from a two-dimensional body placed in uniform stream. We calculated the temperature distribution in the boundary layer and the local heat-transfer coefficient on the body surface under the following assumptions : (1) The outer layer velocity U is expressed by [numerical formula] (2) The body surface temperature distribution A is expressed by [numerical formula] (3) Flow is incompressible. (4) The density ρis independent of temperature. (5) The kinetic viscosity νand the thermal diffusivity α are the linear functions of temperature.
Phosphorus-31 nuclear magnetic resonance probes for the combining site of the myeloma protein M315 (1979)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPhosphorus-31 nuclear magnetic resonance probes for the combining site of the myeloma protein M315S. K. Dower and R. A. DwekCite this: Biochemistry 1979, 18, 17, 3668–3674Publication Date (Print):August 21, 1979Publication History Published online1 May 2002Published inissue 21 August 1979https://pubs.acs.org/doi/10.1021/bi00584a005https://doi.org/10.1021/bi00584a005research-articleACS PublicationsRequest reuse permissionsArticle Views27Altmetric-Citations7LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Ordering of iron vacancies in monoclinic jarosites (2010)

The results of in situ synchrotron X-ray powder diffraction experiments conducted during the synthesis of iron-deficient Na+/H3O+ and K+/H3O+ jarosites at temperatures in the range 80 to 120 °C are presented. They demonstrate that samples can be prepared in which the iron-site vacancies are ordered. The ordering is accompanied by a lowering of symmetry, from rhombohedral, space group R3̅m, to monoclinic, C2/m. The implications for magnetic properties are discussed.
ChemInform Abstract: Synthesis of 11‐Methoxy Anthracyclines. (1992)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Regulation of AHL Production and Its Contribution to Epiphytic Fitness in <i>Pseudomonas syringae</i> (2004)

Pseudomonas syringae forms large cell aggregates that are more stress tolerant than solitary cells during epiphytic growth on plants. The differential survival of aggregates on leaves suggests that epiphytic fitness traits may be controlled in a density-dependent manner via cell-cell signaling. We investigated this hypothesis in P. syringae B728a. Synthesis of N-acyl-homoserine lactone (AHL), 3-oxo-hexanoyl homoserine lactone, and the expression of the gene encoding AHL synthase ahlI were maximal at high cell concentrations. The expression of the AHL regulator ahlR, in contrast, was similar at all cell concentrations. A screen of Tn5 mutants revealed that P. syringae B728a requires a novel transcriptional activator for AHL production. This regulator, which belongs to the TetR family, was also required for epiphytic fitness and has been designated AefR (for AHL and epiphytic fitness regulator). The expression of ahlI was greatly reduced in both aefR- and gacA- mutants and was completely restored in either mutant after addition of exogenous AHL. In contrast, the expression of aefR was not reduced in either gacS- or gacA- mutants. Thus, AefR appears to positively regulate AHL production independently of the regulators GacS/GacA and also controls traits in P. syringae B728a that are required for epiphytic colonization.
<i>Analysis of Numerical Methods</i> (1967)

Keywords: analyse ; methodes : numeriques ; equations : lineaires ; calcul : integral ; equations : differentielles Reference Record created on 2005-11-18, modified on 2016-08-08
ChemInform Abstract: Synthesis of Tetra‐ortho‐Substituted Biaryls Using Aryltriolborates. (2011)

Abstract Highly hindered biaryls are synthesized under optimized conditions which allow an efficient coupling of the sterically congested reaction components.
Removal of DNA from RNA (2010)

DNA contamination of RNA preparations can present significant problems, especially for polymerase chain reaction (PCR)-based applications. No RNA extraction procedure excludes DNA entirely. Cytoplasmic RNA can be contaminated with DNA because of nuclei breakage during preparation. Moreover, TRIzol preparations do not exclude plasmids or other small DNA fragments. This article describes the reliable and effective method of eliminating DNA from RNA preparations via DNase digestion. DNase I specifically digests DNA into small oligonucleotides, leaving RNA intact.
Likelihood ratios for modern screening mammography. Risk of breast cancer based on age and mammographic interpretation (1996)

Most abnormal first screening mammography are interpreted as "additional evaluation needed" and are associated with LRs of about 7. Given this low LR, the risk of breast cancer after mammography is primarily influenced by a woman's age-specific risk of breast cancer before mammography. The LRs for screening mammography interpreted as "suspicious for malignancy" are high (about 124) and are associated with a substantial increase in the risk of breast cancer irrespective of age, but these interpretations comprise only a small proportion of abnormal mammography.