gebhart/patrepeval huggingface dataset:
A new class of indolizidine alkaloids from the poison frog, Dendrobates tricolor. X-ray analysis of 8-hydroxy-8-methyl-6-(2'-methylhexylidene)-1-azabicyclo[4.3.0]nonane (1980)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA new class of indolizidine alkaloids from the poison frog, Dendrobates tricolor. X-ray analysis of 8-hydroxy-8-methyl-6-(2'-methylhexylidene)-1-azabicyclo[4.3.0]nonaneJ. W. Daly, T. Tokuyama, T. Fujiwara, R. J. Highet, and I. L. KarleCite this: J. Am. Chem. Soc. 1980, 102, 2, 830–836Publication Date (Print):January 1, 1980Publication History Published online1 May 2002Published inissue 1 January 1980https://pubs.acs.org/doi/10.1021/ja00522a064https://doi.org/10.1021/ja00522a064research-articleACS PublicationsRequest reuse permissionsArticle Views342Altmetric-Citations90LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
High Performance Liquid Chromatography Resolution of Ubiquitin Pathway Enzymes from Wheat Germ (1990)

The highly conserved protein ubiquitin is involved in several cellular processes in eukaryotes as a result of its covalent ligation to a variety of target proteins. Here, we describe the purification of several enzymatic activities involved in ubiquitin-protein conjugate formation and disassembly from wheat germ (Triticum vulgare) by a combination of ubiquitin affinity chromatography and anion-exchange high performance liquid chromatography. Using this procedure, ubiquitin activating enzyme (E1), several distinct ubiquitin carrier proteins (E2s) with molecular masses of 16, 20, 23, 23.5, and 25 kilodaltons, and a ubiquitin-protein hydrolase (isopeptidase) were isolated. Purified E1 formed a thiol ester linkage with (125)I-ubiquitin in an ATP-dependent manner and transferred bound ubiquitin to the various purified E2s. The ubiquitin protein hydrolase fraction was sensitive to hemin, and in an ATP-independent reaction, was capable of removing the ubiquitin moiety from both ubiquitin (125)I-lysozyme conjugates (epsilon-amino or isopeptide linkage) and the ubiquitin 52-amino acid extension protein fusion (alpha-amino or peptide linkage). Using this procedure, wheat germ represents an inexpensive source from which enzymes involved in the ubiquitin pathway may be isolated.
Abstract 16251: Back to Basics: Diagnosing a Hidden Cause of High-Output Cardiac Failure (2014)

An 85-year-old female with a history of AF and recent onset CHF presented with progressive dyspnea and langour. Her history was notable for five episodes of recurrent hematochezia over the previous 6 months. Extensive evaluation was unrevealing necessitating cessation of her anticoagulation. Initial current inpatient physical examination was notable for elevated JVP and a II/VI holosystolic murmur. TTE revealed normal LVEF, RV enlargement with moderately decreased function, pulmonary hypertension, severe tricuspid TR and a dilated IVC. Abdominal ultrasound showed a calcified structure with arterial flow adjacent to the right kidney and possible renal artery aneurysm. Vascular Medicine was consulted. On our physical exam, we noted a loud right sided flank bruit that persisted throughout the cardiac cycle, and abdominal CT revealed a large right renal AV fistula with 4.5 cm renal artery aneurysm. Right heart catheterization to characterize the hemodynamic effect of the fistula revealed elevated filling pres...
High-Efficiency 1-mm$^{2}$ AlGaInP LEDs Sandwiched by ITO Omni-Directional Reflector and Current-Spreading Layer (2007)

A 1-mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> AlGaInP light-emitting diode (LED) sandwiched by an omni-directional reflector (ODR) and current-spreading layer is presented. The vertical-conducting bottom ODR consists of p-GaP, dispersive dot-contacts of Au-AuBe-Au acting as ohmic contacts, an intermediate low-refractive index layer of indium-tin-oxide, and a silver layer. A Si substrate, which acted as a heat sink, was bonded to the ODR-covered LED structure using a metal-to-metal bonding process. It was found that the maximum output power of the ODR-LED on Si reached 304 mW at 650 mA, and the output power did not saturate up to 650-mA injection current. The external quantum efficiency of 31.8% was obtained at 100 mA, and 19.4% achieved even at currents of up to 800 mA. Furthermore, under a forward current of 350 mA, the ODR-LEDs remained highly reliable after 1000-h testing at room temperature
An Automatic Dewpoint Meter for the Determination of Condensable Vapors (1948)

A method for determination of very small concentrations of condensable vapors by measurement of the decrease in reflectivity of a cooled surface on which the vapor has been deposited is described. The method is applicable to mixtures of 2 components, one of which may be water. Use is made of the fact that a vapor will condense at a concentration below its vapor pressure in the presence of a second vapor whose liquid is soluble in that of the first. The most volatile component of the mixture is used in the pure state as a refrigerating agent whose evaporation cools the light-reflecting surface to an accurately regulated temperature which produces condensation of the two components. An instrument embodying these principles is described which quantitatively measures triethylene glycol vapor in concentrations as low as 10−4 mm Hg. The construction is simple and the operation continuous and automatic. The calibration of the instrument is presented. By proper adjustment, automatic compensation for changing atmospheric relative humidity can be secured so as to obtain response only to the degree of saturation of the glycol vapor in air. This device has been used to investigate condensation of vapors on atmospheric nuclei, and as a control instrument to regulate the concentration of triethylene glycol vapor employed as an aerial germicide for prevention of airborne disease transmission.
ChemInform Abstract: Total Synthesis of Antitumor Agent At‐125, (αS,5S)‐α‐Amino‐3‐chloro‐4,5‐dihydro‐ S‐isoxazoleacetic Acid. (1986)

Abstract Die aus dem bekannten Lacton (Ia) erhältichen Derivate (Ib)‐(Id) werden über die entsprechenden Stufen (II) in die offenkettigen Verbindungen (IV) übergeführt.
Nonlinear attenuation and dispersion in human calcaneus <i>in vitro</i>: Statistical validation and relationships to microarchitecture (2015)

Through-transmission measurements were performed on 30 human calcaneus samples in vitro. Nonlinear attenuation and dispersion measurements were investigated by estimating 95% confidence intervals of coefficients of polynomial expansions of log magnitude and phase of transmission coefficients. Bone mineral density (BMD) was measured with dual x-ray absorptiometry. Microarchitecture was measured with microcomputed tomography. Statistically significant nonlinear attenuation and nonzero dispersion were confirmed for a clinical bandwidth of 300-750 kHz in 40%-43% of bone samples. The mean linear coefficient for attenuation was 10.3 dB/cm MHz [95% confidence interval (CI): 9.0-11.6 dB/cm MHz]. The mean quadratic coefficient for attenuation was 1.6 dB/cm MHz(2) (95% CI: 0.4-2.8 dB/cm MHz(2)). Nonlinear attenuation provided little information regarding BMD or microarchitecture. The quadratic coefficient for phase (which is related to dispersion) showed moderate correlations with BMD (r = -0.65; 95% CI: -0.82 to -0.36), bone surface-to-volume ratio (r = 0.47; 95% CI: 0.12-0.72) and trabecular thickness (r = -0.40; 95% CI: -0.67 to -0.03). Dispersion was proportional to bone volume fraction raised to an exponent of 2.1 ± 0.2, which is similar to the value for parallel nylon-wire phantoms (2.4 ± 0.2) and supports a multiple-scattering model for dispersion.
Comparison of Thyroid, Auricular, and Costal Cartilage Donor Sites for Laryngotracheal Reconstruction in an Animal Model (2000)

The use of autogenous thyroid cartilage for LTP compares favorably with that of other methods of reconstruction that use either auricular or costal cartilage in the rabbit model. This technique is a viable alternative for single-stage LTP and has the added advantage of using a single incision.
Humanization and Pre-Clinical Validation of an Anti-HLA-A*02 (2018)

Introduction Achieving transplant tolerance with regulatory T cell (Treg) adoptive immunotherapy is currently under investigation as a therapy to reduce graft rejection and improve long-term outcomes. Traditional approaches involve the of polyclonal Tregs, which are known to be less potent than antigen-specific cells, or antigen-expanded Tregs, which have several technical limitations. We and others have developed an alternate approach to generate antigen-specific Tregs by expressing a chimeric antigen receptor specific for HLA-A*02:01 (A2-CAR). In our initial studies the antigen-binding region (scFV) of the A2-CAR was derived from the mouse BB7.2 hybridoma, which, due to the high degree of homology between HLA molecules, has been reported to bind to HLA-A alleles in addition to *02:01. Here we sought to systematically define the antigen-specificity of the A2-CAR as well as humanize the sequence to minimize the risk of immunogenicity. Methods We designed 20 humanized versions of the A2-CAR and systematically tested them to determine which were highly expressed on the surface of human Tregs and capable of mediating A2-stimulated activation, expansion, and suppression. We also developed a novel method to systematically, and comprehensively test HLA-allele specificity. Results Of the 20 humanized A2-CARs, 10 were expressed on Tregs and retained A*02:01 binding capacity. We used a series of functional screens to define which of these 10 A2-CARs most effectively stimulated Treg activation, proliferation and proliferation. We then took advantage of the Panel Reactive Antibody (PRA) assay (One Lambda) and created a new method to test CAR-expressing Tregs to bind to specific HLA-alleles. We found that the majority of the humanized A2-CARs had a significantly reduced reactivity to binding to alleles other than A*02:01. We also tested the biological relevance of HLA cross reactivity by stimulating A2-CAR expressing Tregs with cell lines expressing HLA alleles that were or were not found to be cross reactive using the PRA assay. Ultimately, six humanized anti-A2 CARs showed the desired properties, with an ability to activate Tregs, bind to HLA-A2 but not to a comprehensive panel of other common A or B alleles. The potent ability of one of these variants to suppress rejection was confirmed in a humanized model of xenogeneic graft-versus-host disease. Conclusion We successfully developed a series of humanized A2-CARs which were comprehensively screened for desirable properties to generate antigen-specific Tregs. This body of pre-clinical data will support the development of a first-in-human clinical trial of A2-CAR-engineered Tregs to prevent organ allograft rejection.
Doping screening of polarization fields in nitride heterostructures (2000)

Using self-consistent tight-binding calculations, we show that modulation doping can be used to screen macroscopic polarization fields in nitride quantum wells. The blue-shift of photoluminescence peak as well as the reduction of radiative recombination lifetime at increasing doping density is explained and correlated to polarization-field screening. The field-induced ionization of the dopants and its relation with alloy composition in the heterostructure barriers is also analyzed.
A Low-Power Active Self-Interference Cancellation Technique for SAW-Less FDD and Full-Duplex Receivers (2017)

An active self-interference (SI) cancellation technique for SAW-less receiver linearity improvement is proposed. The active canceler combines programmable gain and phase in a single stage and is co-designed with a highly-linear LNA, achieving low noise and low power. A cross-modulation mechanism of the SI canceler is identified and strongly suppressed thanks to the introduction of an internal resistive feedback, enabling high effective receiver IIP3. TX leakage of up to −4 dBm of power is suppressed by over 30 dB at the input of the LNA, with benefits for the entire receiver in terms of IIP3, IIP2, and reciprocal mixing. The design was done in a 40 nm CMOS technology. The system, including receiver and active SI canceler, consumes less than 25 mW of power. When the canceler is enabled, it has an NF of 3.9–4.6 dB between 1.7 and 2.4 GHz and an effective IIP3 greater than 35 dBm.
Nanogram-level micro-volume DNA assay based on the monomeric cyanine dye PO-PRO-3 iodide. (1995)

Several commercially available DNA-staining dyes can yield highly sensitive fluorimetric assays under optimized conditions. However, the high cost of most dyes, coupled with the need for elaborate or expensive instrumentation and/or mL sample volumes, makes assays of this type very costly for routine use. We present a rapid, highly sensitive double-stranded DNA assay based on the new and relatively inexpensive monomeric cyanine dye PO-PRO-3. This dye exhibits maximum excitation/emission wavelengths that are compatible with one of the standard filter sets available on multi-well plate fluorimeters such as the Pandex FCA. The assay does not depend on DNA tertiary structure and is relatively insensitive to protein contamination (up to 1 mg/mL protein), although the bound dye fluorescence does diminish significantly at ionic strengths above approximately 25 mM. Under the assay conditions described here, subnanogram detection limits (in 60-microL sample volumes) are achievable. This assay makes high-sensitivity DNA quantification cost-effective and convenient for many routine analytical applications.
Early and late response-to-injury in patients undergoing transradial coronary angiography: arterial remodeling in smokers. (2014)

In active smokers, transradial coronary angiography is associated with delayed early recovery from transient endothelial dysfunction, decreased NOS-dependent vascular regeneration, and late arterial remodeling pointing towards potential harmful effects of transradial coronary angiography on vascular function in distinct subsets of patients.
Stereotactic body radiotherapy (SBRT) and medical inoperability of early stage non-small cell lung cancer (2006)

17074 Background: Early stage NSCLC is commonly diagnosed in elderly patients who often have significant medical co-morbidity. SBRT is emerging as a treatment alternative that can provide 80–97% local control for peripheral tumors. We performed a literature review and a retrospective review of 24 patients referred to Princess Margaret Hospital for consideration of SBRT to assess what criteria were used to determine patient selection for this new treatment. Methods: Published papers reporting treating stage I NSCLC with SBRT were reviewed. Clinical and demographic characteristics of patients referred for consideration of SBRT over the last 15 months were analyzed. All patients were assessed by experienced thoracic surgeons to determine their operability status. Patients were retrospectively scored as average or high risk for surgery on the basis of accepted PFT guidelines (FEV 1 and DLCO <40% predicted); Charlson Index of Comorbidity (CCI) was calculated (score >2 associated with impaired survival). Results: Of 13 papers reporting Stage 1 SBRT results, 8 included details of medical inoperability. 2 papers had strict guidelines for PFTs, in addition to other criteria. In 6 studies reporting 276 pts, the most common reason for inoperability was non-specific medical comorbidity (60%). Our cohort (24 patients) referred for SBRT (median age 75, range 57–89) had a mean tumour size of 2.32cm (S.D. 1.11); 50% of patients had a good (0–1) Zubrod Performance status. Based upon PFT tests alone 10 patients (42%) were high risk and 5 (21%) average risk for surgery. The CCI score was ≥3 in 10 patients (42%). 3 patients who scored average risk and CCI ≤2 were deemed medically inoperable due a single severe medical comorbity. In 23/24 pts the surgeon recommended against surgery; one patient refused surgery. Conclusions: With an aging population and increased detection due to incidental finding or screening, it is important to know that there is effective alternative therapy for patients deemed too high risk for surgical resection. Comorbidity is an important factor influencing surgical mortality; defining explicit criteria for inoperability remains a clinical challenge. No significant financial relationships to disclose.
Law of corresponding state for the transport properties of molten salts (1965)

A theory of corresponding states is developed for the transport properties of i 4 1 .
Predicting postherpetic neuralgia in elderly primary care patients with herpes zoster: Prospective prognostic study (2007)

Postherpetic neuralgia (PHN) is the most frequent complication of herpes zoster (HZ) and difficult to treat. Timely identification of high-risk HZ-patients enables physicians to focus on PHN prevention. To assess which simple to measure factors are independent predictors of PHN, and whether psychosocial and serological/virological parameters have additional predictive value, a prospective cohort study in primary care was conducted. We included 598 elderly (>50 years) consecutive patients with acute HZ (rash <7 days) below sixth cervical dermatome. At baseline demographic, clinical (e.g., duration and severity of pain and rash), psychological (Pain Cognition List [PCL] and Spielberger's Anxiety Inventory), serological (VZV-antibodies) and virological (viremia presence) variables were measured. Blood tests were performed in a random subset of 218 patients. Primary outcome was significant pain (VAS >30 on 0-100 scale) after three months. The final prediction model obtained from multivariable logistic regression was (internally) validated using bootstrapping techniques, and adjusted for optimism. Forty-six (7.7%) patients developed PHN. Independent predictors were age (odds ratio [OR]=1.08 per year), acute pain severity (OR=1.02 per unit), presence of severe rash (OR=2.31), and rash duration before consultation (OR=0.78 per day): area under receiver-operating-characteristic curve [ROC area]=0.77 (95% CI: 0.71-0.82). Of the five PCL scores, only factor V ('trust in healthcare') was an additional predictor (OR=1.01 per unit), though it increased the ROC area with only 0.01 to 0.78. The Spielberger's anxiety scores and serological and virological variables were no additional predictors. Thus, four simple variables can help physicians to timely identify elderly HZ-patients at risk of PHN.
Modeling demagnetization effects in permanent magnet synchronous machines (2010)

This paper presents a permanent magnet model which takes temperature dependencies and demagnetization effects into account. The proposed model is integrated into a magnetic fundamental wave machine model using the modeling language Modelica. For different rotor types permanent magnet models are developed. The simulation results of the Modelica are compared with measurement results. Additionally, the fundamental wave model is compared to a finite element analysis in order to assess the applicability of the proposed model.
Abstract 997: Stability of β-catenin decides the tumor suppressive effect of MRJ (2011)

Abstract Mammalian relative of DnaJ (MRJ), is a member of the heat shock protein 40 family. We have found that MRJ is lost in advanced grades of breast cancer. Functional studies showed that ectopic expression of the large spliced variant of MRJ, MRJ(L), results in reduced malignant properties as indicated by reduced motility, migration, invasion and soft agar colonization. Furthermore, xenograft studies in athymic mice showed that MRJ(L) expression reduced tumor growth and metastasis. Our investigations also revealed that Wnt/β-catenin signaling is one of the pathways impacted by MRJ(L) expression. Specifically, we found that MRJ(L) expression led to enhanced degradation of β-catenin. Our studies showed that MRJ(L) mediated its tumor suppressive effects by upregulation of DKK1, a negative regulator of Wnt/β-catenin signaling, which promotes proteosome mediated degradation of β-catenin. Hence, we hypothesized that introduction of a degradation-resistant form of β-catenin will lead to restoration of malignant attributes of the MRJ(L) expressing cells. S37A mutation of β-catenin has been known to render it insensitive to ubiquitination-mediated proteosomal degradation. We established stable clones co-expressing S37A β-catenin and MRJ(L). Our analysis of these clones showed that the TCF/LEF activity, as a measure of Wnt/β-catenin signaling, was elevated 10 fold, as measured by the increased activity of the luciferase reporter, TOPFlash. We also found that downstream transcriptional target of Wnt/β-catenin signaling, cyclin D1 showed increased expression in S37A β-catenin clones as compared to the MRJ(L) expressors. Synchronized cell cycle analysis demonstrated that cells co-expressing S37A β-catenin and MRJ(L) resulted in marked increase in S phase at 2 and 24 hours after the release of cell cycle block. The MRJ(L) expressors; did not go through an increase in the S phase in the 24 hour period after the release. Analysis of tumor growth in vivo (xenograft model) revealed that S37A β-catenin was able to restore the malignant activity overriding the suppressive effect of MRJ(L). Thus controlled degradation of β-catenin, brought about by MRJ(L), is an important event in maintaining cells in a non-tumorigenic state. Acknowledgement: We acknowledge grant support from the NCI: 1R01CA140472 Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 997. doi:10.1158/1538-7445.AM2011-997
Decision trees and integrated features for computer aided mammographic screening (1997)

Breast cancer is a serious problem, which in the United States causes 43,000 deaths a year, eventually striking 1 in 9 women. Early detection is the only effective countermeasure, and mass mammography screening is the only reliable means for early detection. Mass screening has many shortcomings which could be addressed by a computer-aided mammographic screening system. Accordingly, we have applied the pattern recognition methods developed in earlier investigations of speculated lesions in mammograms to the detection of microcalcifications and circumscribed masses, generating new, more rigorous and uniform methods for the detection of both those signs. We have also improved the pattern recognition methods themselves, through the development of a new approach to combinations of multiple classifiers.