gebhart/patrepeval huggingface dataset:
Identification of rice root proteins regulated by gibberellin using proteome analysis (2004)

ABSTRACT Gibberellin (GA) promoted rice ( Oryza sativa L.) root elongation in a concentration‐dependent manner with roots grown in 0.1 µ m GA 3 being 13.8% longer than controls. On the other hand, the roots of Tan‐ginbozu, a semi‐dwarf cultivar were 69.8% shorter in comparison with Nipponbare, a normal cultivar. Treatments with 10 µ m uniconazole‐P and 10 µ m abscisic acid (ABA) caused decreases in root length in Tan‐ginbozu by 44.6 and 79.2%, respectively. To investigate how GA influences rice root growth, proteome analysis techniques were applied. Extracted proteins were separated by two‐dimensional polyacrylamide gel electrophoresis and analysed using an automated protein sequencer and mass spectrometer. Sixteen proteins show differences in accumulation levels as a result of treatment with GA 3 , uniconazole‐P and ABA treatment and/or the difference between the semi‐dwarf cultivar, Tan‐ginbozu, and normal cultivars. Among these proteins, fructose‐bisphosphate aldolase (EC 4.1.2.13) increased in roots treated with GA 3 , occurred in low levels in Tan‐ginbozu roots, and decreased in roots treated with uniconazole‐P or ABA. Moreover, roots from seedlings grown in 100 µ m glucose were 9.1% longer than controls. These results indicate that increases in fructose‐bisphosphate aldolase activity stimulate the glycolytic pathway and may play an important role in the GA‐induced growth of roots.
Review of Laboratory Contactors for Studying Kinetics of Gas-liquid Reactions (1995)

This paper presents a review of laboratory contactors which have been developed and applied over the past four decades to study the kinetics of gas-liquid reactions. Such reactions are involved in the removal of acidic gases from natural gas by chemical treatment with liquid solvents. In this review, we dis-cuss the salient positive and negative features of several laboratory contactors, starting with the celebrated disc column, rotating drum, laminar jet, wetted wall, and stirred cell apparatuses, and ending with the recently developed multi-bladed spinning reactor and gas - lift bubble column. The possible manipulation of key mass-transfer parameters in these contactors is critically evaluated. This review also compares and contrasts these contactors in terms of ease of construction and operation, nature of physical contact, nature of thermal field, and analysis of experimental data.
Impact of Nanoclay on the pH-Responsiveness and Biodegradable Behavior of Biopolymer-Based Nanocomposite Hydrogels (2019)

This research work deployed free radical polymerization for the development of pH-responsive hybrid nanocomposite hydrogels (NCHs) with the formation of improved interpenetrating networks (IPN). The crosslinked biopolymeric system was composed of (chitosan (CH)/guar gum (GG)/polyol) and a nanofiller (Cloisite 30B). The study was aimed to investigate the role of Cloisite 30B as a nanofiller and linseed oil-derived polyol to induce stable interpenetrating networks in chitosan‒guar gum-based hydrogels. FT-IR analysis confirmed the formation of crosslinked networks with the formation of hydrogen bonds in the synthesized NCHs. Thermogravimetric analysis and differential scanning calorimetry revealed high thermal stability of the NCHs. The hydrolytic and soil burial degradation tests confirmed the biodegradability of the synthesized NCHs. An extraordinarily high swelling capacity in a buffer solution of pH 4.0 and 7.4 demonstrated their pH-responsive behavior. It has been demonstrated that even the minimal addition of polyol to the guar gum-based hydrogels has influenced the stability and characteristic features such as high swelling capacity owing to the formation of interpenetrating networks and the biodegradability of the hydrogels.
ChemInform Abstract: Syntheses and Biological Activities of 1,4‐Iminoalditol Derivatives as α‐L‐Fucosidase Inhibitors (2011)

Abstract Review: 57 refs.
A Pharma Heroic Act that Wasn't Ignored Written and Published by Dr Bobby George (2018)

Book Review
Removal of the Four C-Terminal Glycine-Rich Repeats Enhances the Thermostability and Substrate Binding Affinity of Barley β-Amylase (2000)

Barley beta-amylase undergoes proteolytic cleavage in the C-terminal region after germination. The implication of the cleavage in the enzyme's characteristics is unclear. With purified native beta-amylases from both mature barley grain and germinated barley, we found that the beta-amylase from germinated barley had significantly higher thermostability and substrate binding affinity for starch than that from mature barley grain. To better understand the effect of the proteolytic cleavage on the enzyme's thermostability and substrate binding affinity for starch, recombinant barley beta-amylases with specific deletions at the C-terminal tail were generated. The complete deletion of the four C-terminal glycine-rich repeats significantly increased the enzyme's thermostability, but an incomplete deletion with one repeat remaining did not change the thermostability. Although different C-terminal deletions affect the thermostability differently, they all increased the enzyme's affinity for starch. The possible reasons for the increased thermostability and substrate binding affinity, due to the removal of the four C-terminal glycine-rich repeats, are discussed in terms of the three-dimensional structure of beta-amylase.
Independence of Steroidogenic Capacity and Luteinizing Hormone Receptor Induction in Developing Granulosa Cells* (1978)

The relationship between FSH-induced acquisition of LH/hCG receptors and the steroidogenic capacity of granulosa cells from estrogen-primed hypophysectomized rat ovaries has been examined. Granulosa cells harvested from the immature preantral follicles of animals not treated with FSH (controls) displayed negligible specific human [125I]iodo-hCG binding and produced only minimal amounts of progesterone during 48 h of culture in vitro. Addition of highly purified hFSH or prostaglandin-E2 (PGE2) to the culture medium elicited substantial increases in progesterone production which were not accompanied by measurable increases in [125I]iodo-hCG binding. Treatment with oFSH in vivo for 24 h led to the initiation of antrum formation in many follicles and was accompanied by an 8-10-fold increase in hCG binding by freshly isolated granulosa cells. Basal, hFSH-, and PGE2-stimulated progesterone production during culture was also greater than controls. In contrast, cells from animals receiving oFSH in vivo for only 12 h showed no increase in hCG binding either before or after culture, yet basal and stimulated progesterone production in vitro was significantly greater than controls, indicating that the initiation of steroidogenesis was antecedent to LH/hCG receptor induction. Only those cells obtained after the 24-h in vivo treatment with oFSH produced elevated amounts of progesterone when incubated in the presence of hCG, thereby showing that the observed increases in [125I]iodo-hCG binding reflected the induction of functionally active LH/hCG receptors. Pharmacological stimulation of steroidogenesis by cell suspensions with N,O'-dibutyryl cAMP resulted in consistently high levels of progesterone production irrespective of previous treatment with FSH in vivo. This uniform expression of in vitro steroidogenic capacity occurred in the complete absence of measurable increases in LH/hCG receptors, suggesting that these two fundamental developmental processes are independent phenomena which may be under separate regulation in vivo.
Design of selective catalysts for olefin metathesis (2013)

Olefin metathesis has become a tool for the synthesis of a variety of mols. and materials. In the past several years the\nefficiency and selectivity of the catalysts have greatly improved. Catalysts are now available that allow the precise control of the\ndouble bond geometrh of the product double bond. Organometallic synthesis and computational results have resulted in new\nlevels of selectivity and efficiency.
Infectious complications of pentostatin therapy. (2000)

Managing the infectious complications associated with pentostatin (Nipent), used alone or in combination with other agents in patients with low-grade lymphomas, poses a significant problem for clinicians. Since there is limited experience with these therapies, definitive treatment recommendations concerning prophylaxis cannot be made. The panel members discussed the use of valacyclovir (Valtrex) to provide prophylaxis for herpes zoster, trimethoprim/sulfamethoxazole for Pneumocystis, and acyclovir (Zovirax) for varicella zoster. They also considered combinations of pentostatin with agents such as interferon, rituximab (Rituxan), and chlorambucil (Leukeran) and their effect on the immune system. The biology of B and T cells was discussed, with an emphasis on clinical application.
From definition to implementation: a cross-industry perspective of past, current and future MIST strategies (2011)

The article describes and discusses the evolution of strategies to characterize metabolites in support of safety studies over the last 40 years, as well as future trends. Approaches to derive qualitative and quantitative information on metabolites are described, with a particular focus on the comparison of options to quantify metabolites in the absence of authentic standards. Current strategies to assess metabolite profiles are summarized into four general approaches and compared against a number of key criteria. Potential future strategies are discussed, including the use of clinical samples as the starting point for metabolite investigations, minimizing the need for animal radiolabelled studies and establishing metabolite safety without radiolabelled studies in animals or human.
Target Detection in Clutter Using Adaptive OFDM Radar (2009)

We address the problem of detecting a target moving in clutter environment using an orthogonal frequency division multiplexing (OFDM) radar. The broadband OFDM signal provides frequency diversity to improve the performance of the system. First, we develop a parametric model that accounts for the measurements at multiple frequencies including the Doppler shift. Then, we present a statistical detection test and evaluate its performance characteristics. Based on this, we propose an algorithm to adaptively design the parameters for the next transmitting waveform. Numerical examples illustrate our analytical results, demonstrating the achieved performance improvement due to the OFDM signaling method and adaptive waveform design.
Donor characteristics as risk factors in recipients after transplantation of bone marrow from unrelated donors: the effect of donor age (2001)

The National Marrow Donor Program (NMDP) maintains a registry of approximately 4 million volunteer unrelated donors for patients in need of a stem cell transplant. When several comparably HLA-matched volunteers are identified for a patient, various criteria are used to select a donor. A retrospective analysis of 6978 bone marrow transplantations facilitated by the NMDP from 1987 to 1999 was conducted to study the effects of various donor characteristics on recipient outcome. The evaluation addressed possible effects of donor age, cytomegalovirus serologic status, ABO compatibility, race, sex, and parity on overall and disease-free survival, acute and chronic graft-versus-host disease (GVHD), engraftment, and relapse. Age was the only donor trait significantly associated with overall and disease-free survival. Five-year overall survival rates for recipients were 33%, 29%, and 25%, respectively, with donors aged 18 to 30 years, 31 to 45 years, and more than 45 years (P =.0002). A similar effect was observed among HLA-mismatched cases (28%, 22%, and 19%, respectively). A race mismatch between recipient and donor did not affect outcome. The cumulative incidences of grade III or IV acute GVHD were 30%, 34%, and 34%, respectively, with donors aged 18 to 30 years, 31 to 45 years, and more than 45 years (P =.005). The corresponding incidences of chronic GVHD at 2 years were 44%, 48%, and 49% (P = 0.02). Recipients with female donors who had undergone multiple pregnancies had a higher rate of chronic GVHD than recipients with male donors (54% versus 44%; P <.0001). The use of younger donors may lower the incidence of GVHD and improve survival after bone marrow transplantation. Age should be considered when selecting among comparably HLA-matched volunteer donors.
Sustainable PHBV/Cellulose Acetate Blends: Effect of a Chain Extender and a Plasticizer (2020)

Poly(3-hydroxybutyrate-<i>co</i>-3-hydroxyvalerate) (PHBV) and cellulose acetate (CA) were blended in the presence of a plasticizer, i.e., triethyl citrate (TEC), and a chain extender, i.e., poly(styrene-acrylic-<i>co</i>-glycidyl methacrylate). To increase the ductility and impact properties of PHBV and to investigate a new biodegradable PHBV-based blend for sustainable packaging, CA was compatibilized with TEC. PHBV and plasticized CA (pCA) blends showed complete immiscibility through separate glass transition and melting peak temperatures in differential scanning calorimetry (DSC), despite the similar Hansen solubility parameters of PHBV, CA, and TEC, indicating partial miscibility. Phase separation between PHBV and pCA was clearly observed by scanning electron microscopy (SEM). PHBV/pCA (70:30) blends had improved impact strength, exceeding that of neat PHBV and pCA, which is attributed to PHBV porosity induced by degradation from the high processing temperature. During processing, the plasticizer migrated from CA to PHBV and partially plasticized it, as evidenced through DSC analysis. The melt temperature of PHBV was reduced, which was confirmed by double melting peaks, representing the formation of secondary crystallites at a lower temperature. Due to processing at high temperatures (210-220 °C), significant porosity was observed in the PHBV/pCA 30:70 blend in SEM analysis. Consequently, the impact strength was improved by 110% as compared to that of virgin PHBV. The addition of CE had no effect on the mechanical properties but did make the PHBV/pCA blends morphologically uniform.
Pre-Strained Epimuscular Connections Cause Muscular Myofascial Force Transmission to Affect Properties of Synergistic EHL and EDL Muscles of the Rat (2005)

Background: Myofascial force transmission occurs between muscles (intermuscular myofascial force transmission) and from muscles to surrounding nonmuscular structures such as neurovascular tracts and bone (extramuscular myofascial force transmission). The purpose was to investigate the mechanical role of the epimuscular connections (the integral system of inter- and extramuscular connections) as well as the isolated role of extramuscular connections on myofascial force transmission and to test the hypothesis, if such connections are prestrained. Method of approach: Length-force characteristics of extensor hallucis longus (EHL) muscle of the rat were measured in two conditions: (I) with the neighboring EDL muscle and epimuscular connections of the muscles intact: EDL was kept at a constant muscle tendon complex length. (II) After removing EDL, leaving EHL with intact extramuscular connections exclusively. Results: (I) Epimuscular connections of the tested muscles proved to be prestrained significantly. (1) Passive EHL force was nonzero for all isometric EHL lengths including very low lengths, increasing with length to approximately 13% of optimum force at high length. (2) Significant proximodistal EDL force differences were found at all EHL lengths: Initially, proximal EDL force =1.18±0.11N, where as distal EDL force =1.50±0.08N (mean ± SE). EHL lengthening decreased the proximo-distal EDL force difference significantly (by 18.4%) but the dominance of EDL distal force remained. This shows that EHL lengthening reduces the prestrain on epimuscular connections via intermuscular connections; however; the prestrain on the extramuscular connections of EDL remains effective. (II) Removing EDL muscle affected EHL forces significantly. (1) Passive EHL forces decreased at all muscle lengths by approximately 17%. However, EHL passive force was still nonzero for the entire isometric EHL length range, indicating pre-strain of extramuscular connections of EHL. This indicates that a substantial part of the effects originates solely from the extramuscular connections of EHL. However, a role for intermuscular connections between EHL and EDL, when present, cannot be excluded. (2) Total EHL forces included significant shape changes in the length-force curve (e.g., optimal EHL force decreased significantly by 6%) showing that due to myofascial force transmission muscle length-force characteristics are not specific properties of individual muscles. Conclusions: The pre-strain in the epimuscular connections of EDL and EHL indicate that these myofascial pathways are sufficiently stiff to transmit force even after small changes in relative position of a muscle with respect to its neighboring muscular and nonmuscular tissues. This suggests the likelihood of such effects also in vivo.
Generalized Hooke's law for isotropic second gradient materials (2010)

In the spirit of Germain the most general objective stored elastic energy for a second gradient material is deduced using a literature result of Fortuné & Vallée. Linear isotropic constitutive relations for stress and hyperstress in terms of strain and strain-gradient are then obtained proving that these materials are characterized by seven elastic moduli and generalizing previous studies by Toupin, Mindlin and Sokolowski. Using a suitable decomposition of the strain-gradient, it is found a necessary and sufficient condition, to be verified by the elastic moduli, assuring positive definiteness of the stored elastic energy. The problem of warping in linear torsion of a prismatic second gradient cylinder is formulated, thus obtaining a possible measurement procedure for one of the second gradient elastic moduli.
Correlation of Well Activity to Time-lapsed Signatures in the Valhall Field for Enhanced Dynamic Interpretation (2010)

We propose an innovative approach that uses well data to constrain the 4D seismic response and help reduce noise levels. Implementation of this well-centric approach requires multiple seismic surveys shot at frequent intervals over the same reservoir. There are now many fields for which such datasets have become available. One such example has been shown previously by Huang and MacBeth (2009) for the non-compacting Schiehallion field. Here we show a further application to the Life of Field Seismic (LoFS) data acquired over the compacting chalk of the Valhall field. The resultant signals identify only those areas of the seismic which are strongly consistent with the well activity, and hence define portions of the reservoir connected to the wells. This information unites the seismic and well domains without the use of the simulation model. The signal tends to be robust and have a much lower noise when compared to the individual 4D seismic difference signatures. This seismic attribute can be used as a diagnostic tool for examining reservoir connectivity and constraining the simulation model.
Unsupervised feature extraction based on improved Wasserstein generative adversarial network for hyperspectral classification (2019)

International audience
Lithium homeostasis in Xenopus oocytes: Implications for the study of signal transduction (1998)

The Xenopus oocyte has been shown to be a useful model for the study of signal transduction pathways. The present study investigated whether or not the oocyte could be used to study the effects of lithium on signal transduction mechanisms by comparing the dynamics of lithium homeostasis in the oocyte and a human immortalized hippocampal cell line using Flame Atomic Emission Spectroscopy (FAES). A biphasic pattern of lithium uptake was observed in the oocyte in the presence of 5 mM extracellular lithium. The late phase of lithium uptake, which started after 30 minutes of incubation time, was sensitive to phloretin, an inhibitor of Na+/Li+ counter-transport. Differences in lithium efflux kinetics further characterized the two observed phases of accumulation and also suggested that lithium might be distributed in different pools within the oocyte, including one sequestered in organelles or associated with cytosolic proteins. An analogous sequestered pool was not, however, observed in the hippocampal cell line indicating that lithium is distributed differently in these cell types. This suggests that the Xenopus oocyte might not be a suitable model for evaluating the effects of lithium on signal transduction pathways because of the unknown contribution of the sequestered pool on predicting relevant physiological effects.
Novel Dimeric Nur77 Signaling Mechanism in Endocrine and Lymphoid Cells (1997)

Within the nuclear receptor family, Nur77 (also known as NGFI-B) distinguishes itself by its ability to bind a target sequence (the NBRE) as a monomer and by its role in T-cell receptor (TCR)-induced apoptosis in T cells. We now report on a novel mechanism of Nur77 action that is mediated by homodimers. These dimers bind a Nur77 response element (NurRE), which has been identified as a target of CRH-induced Nur77 in the pro-opiomelanocortin (POMC) gene promoter. Both halves of the palindromic NurRE are required for responsiveness to physiological signals, like CRH in pituitary-derived AtT-20 cells. Similarly, in T-cell hybridomas, TCR activation induced NurRE but not NBRE reporters. The in vivo signaling function of Nur77 thus appears to be mediated by dimers acting on a palindromic response element of unusual spacing between its half-sites. This mechanism may represent the biologically relevant paradigm of action for this subfamily of orphan nuclear receptors.
Fluorescence Probe Studies of Hydrophobic Domains in a Model Hydrophobically Modified Alkali-Swellable Emulsion (HASE) Polymer with C<sub>20</sub>H<sub>41</sub> Groups (1999)

International audience
Mutational Analysis of the <i>vacA</i> Promoter Provides Insight into Gene Transcription in <i>Helicobacter pylori</i> (1999)

Analysis of 12 Helicobacter pylori promoters indicates the existence of a consensus -10 hexamer (TAtaaT) but little conservation of -35 sequences. In this study, mutations in either the H. pylori vacA -10 region or the -35 region resulted in decreased vacA transcription and suggested that an extended -10 motif is utilized. Thus, despite the lack of a -35 consensus sequence for H. pylori promoters, the -35 region plays a functional role in vacA transcription.
Loss of cell cycle control allows selective microtubule-active drug-induced Bcl-2 phosphorylation and cytotoxicity in autonomous cancer cells. (2000)

Lack of selectivity in the killing of tumor and normal cells is a major obstacle in cancer therapy. By inhibiting normal but not autonomous cell growth, we exploited the differences in cell cycle regulation to achieve a selective protection of nonautonomous cells against paclitaxel and other microtubule-active drugs. Tubulin polymerization, a primary effect of paclitaxel, can be dissociated from Bcl-2 phosphorylation and cytotoxicity in HL-60 cells. Growth arrest prevented paclitaxel-induced Bcl-2 phosphorylation and apoptosis without affecting paclitaxel-induced tubulin polymerization. We abrogated the effects of paclitaxel on MCF-10A immortalized breast cells, while preserving its effects on MCF-7 cancer cells. Unlike MCF-7 cells, MCF-10A cells were arrested by epidermal growth factor withdrawal, precluding paclitaxel-induced Bcl-2 phosphorylation. Furthermore, the inhibition of the epidermal growth factor receptor kinase with low doses of AG1478 arrested growth of MCF-10A but not MCF-7 cells. Pretreatment with AG1478 did not affect paclitaxel-induced Bcl-2/Raf-1 phosphorylation in MCF-7 but abrogated such phosphorylation in MCF-10A. Exploitation of growth factor dependency may allow the protection of normal cells from microtubule-active drugs.