gebhart/patrepeval huggingface dataset:
The inhibitory guanine nucleotide-binding regulatory component of adenylate cyclase. Properties and function of the purified protein. (1984)

Treatment of membranes with islet activating protein (IAP), a toxin from Bordetella pertussis, results in abolition of GTP-dependent, receptor-mediated inhibition of adenylate cyclase. This appears to result from IAP-catalyzed ADP-ribosylation of a 41,000-Da membrane-bound protein. A protein with 41,000- and 35,000-Da subunits has been purified from rabbit liver membranes as the predominant substrate for IAP. This protein has now been shown to be capable of regulating membrane-bound adenylate cyclase activity of human platelets under various conditions. The characteristics of the actions of the IAP substrate are as follows. 1) Purified 41,000/35,000-Da dimer is capable of restoring the inhibitory effects of guanine nucleotides and the alpha 2-adrenergic agonist, epinephrine, on the adenylate cyclase activity of IAP-treated membranes. 2) The subunits of the dimer dissociate in the presence of guanine nucleotide analogs or A1(3+), Mg2+, and F-. The 41,000-Da subunit has a high affinity binding site for guanine nucleotides. 3) The resolved 35,000-Da subunit of the dimer mimics guanine nucleotide- and epinephrine-induced inhibition of adenylate cyclase. 4) The resolved (unliganded) 41,000-Da subunit stimulates adenylate cyclase activity and relieves guanine nucleotide- +/- epinephrine-induced inhibition of the enzyme. In contrast, the GTP gamma S-bound form of the 41,000-Da subunit inhibits adenylate cyclase activity, although with lower apparent affinity than does the 35,000-Da subunit. 5) The 35,000-Da subunit increases the rate of deactivation of Gs, the stimulatory regulatory protein of adenylate cyclase. In contrast, the 41,000-Da subunit can interact with Gs and inhibit its deactivation. These data strongly suggest that the IAP substrate is another dimeric, guanine nucleotide-binding regulatory protein and that it is responsible for inhibitory modulation of adenylate cyclase activity.
Electron spin resonance studies of copper(2+) ion doped in single crystals of cytosine monohydrate (1975)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTElectron spin resonance studies of copper(2+) ion doped in single crystals of cytosine monohydrateKazumi Toriyama and Machio IwasakiCite this: J. Am. Chem. Soc. 1975, 97, 22, 6456–6460Publication Date (Print):October 1, 1975Publication History Published online1 May 2002Published inissue 1 October 1975https://pubs.acs.org/doi/10.1021/ja00855a026https://doi.org/10.1021/ja00855a026research-articleACS PublicationsRequest reuse permissionsArticle Views77Altmetric-Citations9LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
[P3–214]: ADFLAG<sup>®</sup>, A DIAGNOSTIC BLOOD TEST FOR PRE‐DEMENTIA STAGES OF ALZHEIMER's DISEASE (2017)

Alzheimer's disease (AD) affects 47.5 million people worldwide1. Despite significant efforts, no treatments are available due to decades of failure in DMD development. One of the reasons for these failures lies in the lack of efficient patient stratification tools to support demonstration of DMD efficacy in pre-dementia patients. In 2016, Bruno et al.2proposed a new A/T/N system in which 7 major AD biomarkers are divided into 3 binary categories based on the nature of the pathophysiology. This new classification identifies incident AD. We have previously shown that the ADFlag® blood test is capable of segregating pre-dementia AD-patients. Here we sought to study the relationship between ADFlag® classification with respect to the A/T/N criteria. ADFlag® is a non-invasive test measuring inflammatory proteins in the blood. ADFlag® pilot was devised using ELISA and FACS, to facilitate its adoption in clinical reference laboratories. Initial POC was obtained in 298 patients originating from the Pharmacog multicentric, and from the Alzpredict monocentric cohorts. The level of expression of the ADFlag® protein panel was measured in all blood samples, and classified into 5 pre-symptomatic groups with 82–92% accuracy consistently with neuropsychological assessments. In the Pharmacog cohort, using the A/T/N classification, prodromal AD patients were classified into 3 groups (Positive, negative and intermediate)3. Our study demonstrated an association between the ADFlag® scale and the scores based on the ratio of circulating amyloid beta and Tau or pTau CSF levels. Analysis of variance showed that an ADFlag® score of 5 was present at a higher frequency in patients with low Abeta/high Tau (Chisq=0.0195) or pTau (ChiSq=0.0224). Moreover, the ADFlag scale was able to segregate the “intermediate” populations into 3 groups, that could be distinguished based on their inflammatory profile. We also found that CANTAB paired associated learning errors, RAVLT scores and word recognition task scores changes significantly with he ADFlag® scale demonstrating a good coherence of this novel stratification tool with visual memory and new learning including verbal learning and memory. ADFlag® panel will help for stratification for clinical preventive trials and develop a companion diagnostic for anti-inflammatory DMD in AD.
[Combination with medroxyprogesterone acetate and chemotherapy making possible complete removal of uterine corpus carcinoma]. (1989)

A case of uterine corpus carcinoma (stage III) after surgical resection of her primary lesion was treated with a medroxyprogesterone acetate (MPA) and PAE (CDDP, adriamycin and etoposide) regimen. The patient has been given MPA during a three-month period and received four courses of PAE regimen. The residual tumor was decreased and removed completely at the second-look operation. Pathologically, the carcinoma cells were clearly changed in loss of atrophy or degeneration by MPA or PAE regimen. After the second-look operation, the patient underwent 4 courses of PAE regimen and was given MPA continuously. We have not recognized any recurrence of carcinoma for seven months after the second look operation.
New Prenylated Benzoic Acid and Other Constituents from Almond Hulls (<i>Prunus amygdalus</i> Batsch) (2001)

One new prenylated benzoic acid derivative, 3-prenyl-4-O-beta-D-glucopyranosyloxy-4-hydroxylbenzoic acid, and three known constituents, catechin, protocatechuic acid, and ursolic acid, have been isolated from the hulls of almond (Prunus amygdalus). Complete assignments of the proton and carbon chemical shifts for the new prenylated benzoic acid derivative were accomplished on the basis of high-resolution 1D and 2D nuclear magnetic resonance data. All of these compounds except ursolic acid are being reported from almond hulls (P. amygdalus) for the first time.
Cardiac Muscle Ring Finger‐1 (MuRF1) alters myocardial ROS production <i>in vivo</i> (2011)

Muscle ring finger protein‐1 (MuRF1) is a muscle‐specific ubiquitin ligase that regulates cardiac metabolism at multiple points in vivo . We have identified these mechanisms using transgenic (MuRF1 Tg+) and knockout mice (MuRF1 −/−). Microarray analysis of the MuRF1 Tg+ hearts revealed significant alterations in genes involved in mitochondrial oxidative phosphorylation. We therefore investigated the effects of cardiac MuRF1 expression on mitochondrial function. Isolated permeabilized muscle fibers from MuRF1 Tg+ and MuRF1 −/− hearts were assayed for their oxygen utilization in the presence of a number of Kreb's cycle intermediates. Mitochondria O 2 consumption from MuRF1 Tg+ and MuRF1 −/− mice did not significantly differ from wild type mice in the presence of pyruvate, malate, glutamate, and succinate (+/−ADP), although MuRF1 Tg+ trended to consume less oxygen. Mitochondria uncoupling was not detected in the presence of oligomycin in either mouse model. Quite unexpectedly, however, mitochondrial hydrogen peroxide production determined by Amplex Red was significantly reduced by >60% in MuRF1 Tg+ hearts, indicating increased MuRF1 inhibited the formation of reactive oxygen species. These findings are consistent with MuRF1's cardioprotective role in ischemia reperfusion injury, whereby the formation of ROS play a critical role in the pathogenesis of disease. Supported by NIH R01HL104129.
Poling of Ferroelectric PT/P(VDF-TrFE) 0-3 Composite (2002)

Abstract Thick-film composites with 5 % to 30 % volume fraction of nanocrystalline lead titanate (PT) ceramic particles dispersed in a 70/30 mol-% vinylidene fluoride-trifluoroethylene [P(VDF-TrFE)] copolymer matrix were prepared by solution casting followed by compression moulding. The crystal structure of the PT particles and the microstructure of the composite were characterized by XRD and SEM, respectively. The composites were poled at elevated temperature to produce samples with only the ceramic phase polarized. The poling current was recorded during the poling process, and a roughly exponential decay has been observed with "time constants" in the order of 10 3 s. The pyroelectric coefficient has been studied as a function of poling time. The built-up of the pyroelectric coefficient is faster than the decay of the poling current. Among composites with different ceramic loading the polarization develops with a higher rate in the composites with higher ceramic volume fraction. The polarization profiles of the poled composites have also been studied using the laser intensity modulation method (LIMM). Composite films with 30 % ceramic volume fraction poled for 10 3 s show a uniform polarization profile along the thickness direction, except in regions very close to the surfaces. The poling state of the ceramic inclusions was investigated by XRD. Intensity changes in the (002) and (200) diffraction peaks after poling reflect 90 domain switching in the PT inclusions. The poling ratio was estimated from the intensities of the diffraction peaks. A higher poling ratio of the ceramic inclusions was obtained in composites with higher ceramic volume fraction. Keywords: Lead Titanate0-3 CompositePolarizationPyroelectricLimm
Cleaning of MBE GaAs Substrates by Hydrogen Radical Beam Irradiation (1987)

Hydrogen radical beam cleaning of substrates for GaAs MBE growth has been studied. The cleaning effects of carbon and oxygen contaminated by exposure to air and a lower-grade vacuum were investigated using Auger electron spectroscopy (AES), capacitance-voltage (C-V) carrier profiling, and secondary ion mass spectrometry (SIMS). AES signals of contaminants decreased to the detection limit with hydrogen radical irradiation. Carrier depletion around the growth interface was also reduced with decreasing carbon density.
[Physiology of intestinal absorption of phosphorus in animals]. (1995)

Intestinal absorption of inorganic phosphorus. In most mammalian species inorganic phosphorus (P) is absorbed at the duodenal and jejunal level. However in horses some P is absorbed from the large intestine, whereas in ruminant animals some absorption also takes place in the forestomachs. The structure of the putative phosphate-sodium carrier of the brush border from enterocytes still awaits identification. P absorption is modulated both by endocrine (calcitriol, triiodothyronine) and nutritional factors (minerals of the diet, chemical form of phosphorus). Regulation of salivary P secretion, intestinal absorption of P in ruminants, and its adaptation to diets high or low in P are still poorly understood. Such studies would probably help to decrease the cost of P supplementation in farm animals.
Kinetics and mechanism of a fast leuco-Methylene Blue oxidation by copper(ii)–halide species in acidic aqueous media (2002)

The kinetics of a fast leuco-Methylene Blue (LMB) re-oxidation to Methylene Blue (MB) by copper(II)–halide (Cl−, Br−) complexes in acidic aqueous media has been studied spectrophotometrically using a stopped-flow technique. The reaction follows a simple first order rate expression under an excess of the copper(II) species (and H+(aq)), and the pseudo-first order rate constant (k′obs) is largely independent of the atmosphere used (air, oxygen, argon). The rate law, at constant Cl− (Br−) anion concentration, is given by the expression: (d[MB+])/dt = ((kaK[H+] + kb)/(1 + K[H+]))·[CuII][LMB] = k′obs[LMB], where K is the protonation constant, and ka and kb are the pseudo-second order rate constants for protonated and deprotonated forms of LMB, respectively. The rate law was determined based on the observed k′obsvs. [CuII] and [H+] dependences. The rate dramatically increases with [Cl−] over the range: 0.1–1.5 M, reflecting the following reactivity order: Cu2+(aq) ≪ CuCl+(aq) < … < CuCl42−. The slow re-oxidation of LMB by oxygen has also been briefly examined at different [H+]. ESR results provide clear evidence for the formation of an intermediate radical. The mechanistic consequences of all these results are discussed.
Differences in the Response of Rabbit Small Intestine to Heat-Labile and Heat-Stable Enterotoxins of <i>Escherichia coli</i> (1973)

The response of adult rabbit small intestine to the heat-stable (ST) and heat-labile (LT) enterotoxins of Escherichia coli has been investigated by employing the ligated loop technique. Fluid accumulation was determined in relation to enterotoxin dose and duration of gut exposure. The individual responses to ST and LT differed in a characteristic manner. Onset of net fluid accumulation in response to ST appeared to be immediate even at the lowest dose tested. Onset of net fluid accumulation in response to LT was rapid at high doses but delayed at low doses. Maximum volume per length ratios elicited by ST occurred between 4 and 6 h after injection of loops over the entire range of doses tested. However, maximum ratios elicited by LT occurred no less than 10 h after injection even at low doses. Fluid accumulation elicited by LT increased in duration with increasing dose; high doses of LT producing a response which was sustained for at least 18 h. The net effect of these differences in reaction characteristics is a sharp increase in the proportion of the cumulative net secretory response attributable to LT with time. Therefore, a 6-h assay time is appropriate for the titration of ST, whereas an 18-h assay is not. The 18-h assay was found more appropriate for toxin-antitoxin neutralization studies since only LT was neutralized by anti-enterotoxin serum. LT of E. coli (swine) strain P-263 and (human) strain 334 was neutralized by antibody stimulated by enterotoxin from E. coli (human) strain H-10407. LT was labile to mild acid conditions, whereas ST was not.
A flexible model for the cell wall polysaccharide of Streptococcus mitis J22 determined by three-dimensional 13C edited nuclear overhauser effect spectroscopy and 13C-1H long-range coupling constants combined with molecular modeling (1998)

We report on the conformation of a tetrasaccharide fragment in the repeating subunit of the cell wall polysaccharide of Streptococcus mitis J22, a receptor for the lectin of Actinomyces viscosus T14V in a bacterial coaggregation that is important in the ecological interactions of oral bacteria. Although there is considerable overlap of the 1H-nmr signals, some cross peaks can be extracted from conventional two-dimensional nuclear Overhauser effect spectroscopy (NOESY) data on the polysaccharide. These data cannot be fit to a single conformation of the tetrasaccharide fragment. Therefore we have prepared a polysaccharide sample fully enriched in 13C from which we have determined accurate NOESY cross-peak volumes in a three-dimensional heteronuclear-resolved spectrum that allows accurate determination of many more NOESY cross peaks than does conventional two-dimensional spectroscopy. We have also used the 13C enriched polysaccharide to measure accurate values of long-range 13C-1H coupling constants that can be correlated with glycosidic dihedral angles. Molecular modeling calculations on the polysaccharide fragment, including molecular dynamics simulations, identify multiple low-energy conformations. This result is to be contrasted with previous calculations on blood group oligosaccharides in our laboratory using similar methods that showed relatively rigid conformations with little flexibility of the glycosidic linkages. The present NOESY and 3JCH data can be reconciled with a model for the antigenic tetrasaccharide in which three distinct conformations are in fast exchange. We propose that some carbohydrate epitopes such as those of the blood group oligosaccharides are relatively rigid while others such as the tetrasaccharide fragment in these studies exhibit much greater flexibility.
Entropy Generation Due to Laminar Incompressible Forced Convection Flow Through Parallel-Plates Microchannel (2004)

The entropy generation due to steady laminar forced convection fluid flow through parallel plates microchannel is investigated numerically. The effect of Knudsen, Reynolds, Prandtl, Eckert numbers and the nondimensional temperature difference on entropy generation within the microchannel is discussed. The fraction of the entropy generation due to heat transfer to the total entropy generation within the microchannel is studied in terms of Bejan number. The entropy generation within the microchannel is found to decrease as Knudsen number increases, and it is found to increase as Reynolds, Prandtl, Eckert numbers and the nondimensional temperature difference increase. The contribution of the viscous dissipation in the total entropy generation increases as Knudsen number increases over wide ranges of the flow controlling parameters.
Matrix thinning with reduced‐field testing (RFT) (1995)

Abstract The reduced‐field testing (RFT) method, presented here, is based on the distinction between near‐field and far‐field interactions in the spatial domain, generating a single, explicit sparse matrix. Basis functions are chosen independently; thus the method can enhance existing codes and be combined with other efficient techniques. © 1995 John Wiley & Sons, Inc.
Functional Repair with Neural Stem Cells (2000)

Approval to commence phase I/II clinical trials with neural stem cells requires proof of concept in well-accepted animal models of human neurological disease or injury. We initially showed that the conditionally immortal MHP36 line of hippocampal origin (derived from the H-2Kb-tsA58 transgenic mouse) was effective in repopulating CA1 neurons in models of global ischaemia and repairing cognitive function, and have now shown that this line is multifunctional. MHP36 cells are effective in restoring spatial memory deficits in rats after excitotoxic lesions of the cholinergic projections to cortex and hippocampus and in rats showing cognitive impairments due to normal ageing. Moreover, grafts of MHP36 cells are effective in reversing sensory and motor deficits and reducing lesion volume as a consequence of occlusion of the middle cerebral artery, the major cause of stroke. In contrast, MHP36 cell grafts were unable to repair motor asymmetries in rats with unilateral 6-hydroxydopamine lesions of the nigrostriatal dopamine system, the prototype rodent model of Parkinson's disease. These data show that conditionally immortal neuroepithelial stem cells are multifunctional, being able to repair diverse types of brain damage. However, there are limitations to this multifunctionality, suggesting that lines from different regions of the developing brain will be required to treat different brain diseases. ReNeuron is currently developing human neuroepithelial stem cell lines from different brain regions and with similar reparative properties to our murine lines.
TRANSFORMATION BY V-RAS ONCOGENE CORRELATES WITH AN INCREASED DRUG-RESISTANCE IN AN EPITHELIAL THYROID-CELL SYSTEM (1995)

The effects exerted by ms transformation upon sensitivity to anticancer drugs by using an epithelial cell system displaying different levels of ras expression which well correlate with different degrees of cell malignancy have been evaluated. The diminished drug accumulation of the transformed cell lines was found to be correlated with a depolarization of the cell membrane as observed by a flow cytometric analysis and not correlated with the multidrug resistance (mdr) protein. The most resistant cell line, MPTK-6, showed the lowest drug accumulation levels and the lowest membrane potential. These observations indicate that ras neoplastic transformation of the rat thyroid epithelial cells modifies the membrane potential and this modification is possibly involved in anticancer drugs resistance.
ChemInform Abstract: Heterogeneous Catalysis: Future Opportunities in a Historical Perspective. (1994)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Ensembles of Similarity-Based Models (2001)

Ensembles of independent classifiers are usually more accurate and show smaller variance than individual classifiers. Methods of selection of Similarity Based Models (SBM) that should be included in an ensemble are discussed. Standard k-NN, weighted k-NN, ensembles of weighted models and ensembles of averaged weighted models are considered. Ensembles of competent models are introduced. Results of numerical experiments on benchmark and real-world datasets are presented.
Aortic Stenosis—50 Years of Discovery (2018)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Cardiology HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Cardiology journal
狭ピッチ化を目指したAl-Si/TiNバンプによるCoC接合 (2018)

３次元接合技術は、高速化・高集積化・低消費電力化に対して大きなメリットがある。イメージセンサに対しては、上記のメリットの他に高開口率化が付け加えられる。我々はイメージセンサの高開口率化・高解像度化を進めるために、狭ピッチでCoC接続可能なAl-Si/TiNバンプを製作し、３次元接合の評価を行った。その結果、我々がこれまで利用してきたAuスタッドバンプより良い結果を取得する事ができた。