gebhart/patrepeval huggingface dataset:
From Hong Kong Diabetes Register to JADE Program to RAMP-DM for Data-Driven Actions (2019)

In 1995, the Hong Kong Diabetes Register (HKDR) was established by a doctor-nurse team at a university-affiliated, publicly funded, hospital-based diabetes center using a structured protocol for gathering data to stratify risk, triage care, empower patients, and individualize treatment. This research-driven quality improvement program has motivated the introduction of a territory-wide diabetes risk assessment and management program provided by 18 hospital-based diabetes centers since 2000. By linking the data-rich HKDR to the territory-wide electronic medical record, risk equations were developed and validated to predict clinical outcomes. In 2007, the HKDR protocol was digitalized to establish the web-based Joint Asia Diabetes Evaluation (JADE) Program complete with risk levels and algorithms for issuance of personalized reports to reduce clinical inertia and empower self-management. Through this technologically assisted, integrated diabetes care program, we have generated big data to track secular trends, identify unmet needs, and verify interventions in a naturalistic environment. In 2009, the JADE Program was adapted to form the Risk Assessment and Management Program for Diabetes Mellitus (RAMP-DM) in the publicly funded primary care clinics, which reduced all major events by 30-60% in patients without complications. Meanwhile, a JADE-assisted assessment and empowerment program provided by a university-affiliated, self-funded, nurse-coordinated diabetes center, aimed at complementing medical care in the community, also reduced all major events by 30-50% in patients with different risk levels. By combining universal health coverage, public-private partnerships, and data-driven integrated care, the Hong Kong experience provides a possible solution than can be adapted elsewhere to make quality diabetes care accessible, affordable, and sustainable.
A Randomized, Controlled Trial of Oral Intestinal Sorbent AST-120 on Renal Function Deterioration in Patients with Advanced Renal Dysfunction (2016)

Long-term use of AST-120 added to standard treatment did not change renal disease progression, proteinuria, mortality, and health-related quality of life in patients with advanced renal dysfunction.
A versatile reporter system for multiplexed screening of effective epigenome editors (2020)

The formation and function of highly specialized cells and tissues in a multicellular organism from a single genome are enabled through differential spatiotemporal access to the information contained in the genomic DNA. The epigenome plays an essential role in how DNA information can be accessed, and in the last decade the link between epigenetic aberrations and pathologies has become increasingly clear. Methods to precisely modify the epigenome are hence attracting interest as potential novel therapeutics. We recently described a platform, designer epigenome modifier (DEM), capable of precisely editing the epigenome of a cell to control the expression of selected genes. Here, we provide a detailed protocol to streamline the process of identifying DEMs that efficiently and selectively bind to their intended target site and inactivate expression of the target gene. Further, we describe the procedure to simultaneously regulate the expression of up to three genes in a multiplexed fashion. The protocol is divided into four stages that guide the user through the generation of the multicolor reporter cell line and its use for selecting functional DEMs. The duration of the whole procedure described varies from ~6 weeks when using a single reporter up to 13 weeks for fine-tuning the multiplex epigenome editing abilities of selected DEMs using three reporters. Given the great interest in epigenome editing in various fields of biomedical research, this protocol will help scientists to explore these novel technologies for their research.
Developmental Growth Control Exerted via the Protein A Kinase Tpk2 in Ashbya gossypii (2015)

Sporulation in Ashbya gossypii is induced by nutrient-limited conditions and leads to the formation of haploid spores. Using RNA-seq, we have determined a gene set induced upon sporulation, which bears considerable overlap with that of Saccharomyces cerevisiae but also contains A. gossypii-specific genes. Addition of cyclic AMP (cAMP) to nutrient-limited media blocks sporulation and represses the induction of sporulation specific genes. Deletion of the protein kinase A (PKA) catalytic subunits encoded by TPK1 and TPK2 showed reduced growth in tpk1 but enhanced growth in the tpk2 strain; however, both mutants sporulated well. Sporulation can be blocked by cAMP in tpk1 but not in tpk2 strains. Similarly, TPK2 acts at a second developmental switch promoting the break in spore dormancy. In S. cerevisiae, PKA phosphorylates and inhibits Msn2/4. The transcript profiles of the tpk1 and msn2/4 mutants were very similar to that of the wild type under sporulation conditions. However, deletion of the single A. gossypii MSN2/4 homolog generated a specific sporulation defect. We identified a set of genes involved in spore wall assembly that was downregulated in the msn2/4 mutant, particularly DIT2, suggesting that poor spore viability may be due to lysis of spores. Our results reveal specific functional differences between the two catalytic PKA subunits in A. gossypii and identified Tpk2 as the key A kinase that transduces developmental decisions of growth. Our data also suggest that Msn2/4 is involved only at a late step of sporulation in A. gossypii and is not a major regulator of IME1.
Structural, electrical, and magnetic properties of chemical solution deposited BiFe1−xTixO3 and BiFe0.9Ti0.05Co0.05O3 thin films (2009)

BiFeO 3 (BFO), BiFe1−xTixO3, and BiFe0.9Ti0.05Co0.05O3 thin films were deposited on Pt/TiO2/SiO2/Si substrates by chemical solution deposition. BFO film has distorted rhombohedral R3c structure and in BiFe1−xTixO3 (104)/(110) reflections broadened suggesting limited grain growth with Ti substitution. The surface roughness (rms) decreased in the case of Ti substituted BFO. Up to 5% Ti in the lattice reduces the leakage current substantially. For BiFe1−xTixO3, the leakage current qualitatively followed the same trend and the behavior resembles to space charge limited current conduction. The magnetic properties were completely lost by Ti substitution and slightly recovered upon cosubstitution with magnetically active Co. The disappearance of ferromagnetic hysteresis of BFO with Ti substitution and its reappearance with Co is suggestive of the origin of magnetic properties consequential from the BFO lattice itself and hence support it as an intrinsic property of BFO. Capacitance-voltage characteristics of BFO, BiFe0.95Ti0.05O3, and BiFe0.9Ti0.05Co0.05O3 showed butterfly loop indicating ferroelectric property at room temperature as well as at low temperature. However, saturated polarization-voltage hysteresis was not observed in all cases and BiFe0.9Ti0.05Co0.05O3 films showed very poor ferroelectricity compared to BFO and BiFe0.95Ti0.05O3.
Shape resonances in the angle-resolved photoelectron spectroscopy of the Si 2<i>p</i> shell of SiCl4 (1986)

The partial cross sections and angular distribution parameters, β, have been determined for the 2p subshell in SiCl4. These data were obtained with angle-resolved photoelectron spectroscopy and the use of synchrotron radiation for a range of photon energies from 114 to 150 eV. The same quantities were calculated by use of a multiple scattering Xα method, and the agreement between experiment and theory is very good. In particular, the behavior of shape resonances was well predicted. The nature of shape resonances as a function of molecular orbitals in SiCl4 is discussed, and the importance of such intercomparison is emphasized.
Induction of metallothionein and other mRNA species by carcinogens and tumor promoters in primary human skin fibroblasts. (1986)

We used nucleic acid hybridization and cDNA cloning techniques to isolate human sequences that respond to the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA). These clones were used as probes to examine changes of gene expression that occurred after the proliferation of exponentially growing primary human fibroblasts was arrested. Transcript levels detected by these probes were increased coordinately by treatment of the cells with UV light, mitomycin C, TPA, or the UV light-induced extracellular protein synthesis-inducing factor EPIF (M. Schorpp, U. Mallick, H. J. Rahmsdorf, and P. Herrlich, Cell 37:861-868, 1984). Proteins coded for by these transcripts were characterized by hybrid-promoted translation and by cDNA sequencing. One of the cDNA clones was homologous to the metallothionein IIa gene, and one set of related clones selected RNA for the secreted TPA-inducible protein XHF1 (U. Mallick, H. J. Rahmsdorf, N. Yamamoto, H. Ponta, R.-D. Wegner, and P. Herrlich, Proc. Natl. Acad. Sci. USA 79:7886-7890, 1982).
Seismic Protection of Structures using Base Isolation and Active Control (1987)

We propose the use of robust control in conjunction with base isolation in order to assure arbitrarily small motion of a seismically excited structure. The proposed method requires control force application only at the base (first) floor. The efficacy of the scheme is illustrated by extensive simulations for a prototype six-story building.
The Bioactive Conformation of Human Parathyroid Hormone. Structural Evidence for the Extended Helix Postulate (2000)

Conformational restrictions in the form of [i, i + 4] lactam bridges were sequentially incorporated into the shortest fragment of hPTH with recognized efficacy in the OVX rat model of osteoporosis, hPTH(1−31)NH2 (1). Cyclo(Lys18−Asp22)[Ala1,Nle8,Lys18,Asp22,Leu27]hPTH(1−31)NH2 (2) is a potent agonist of the PTH/PTHrP receptor located on the surface of ROS 17/2.8 cells as measured by its ability to stimulate adenylyl cyclase activity (EC50 = 0.29 nM). A second analogue, which constrains the entire C-terminal receptor binding domain, bicyclo(Lys18−Asp22,Lys26−Asp30)[Ala1,Nle8,Lys18,Asp22,Leu27] hPTH(1−31)NH2 (6), is also shown to be a potent agonist (EC50 = 0.13 nM), thus providing further evidence for an extended helix as the relevant bioactive conformation in this region of the hormone. Adjacent lactam bridges were incorporated into the analogue bicyclo(Lys13−Asp17,Lys18−Asp22)[Ala1,Nle8,Lys18,Asp17,22,Leu27]hPTH(1−31)NH2 (7) to evaluate the receptor's tolerance to conformational restriction in the midregion of the peptide. In fact, peptide 7 is also a highly potent agonist (EC50 = 0.43 nM) in the cAMP-based assay, which suggests that at least one bioactive form of the hormone requires a helical conformation extending from residue 13 to residue 22. Incorporation of all three lactam bridges afforded the most conformationally constrained PTH peptide agonist yet reported, tricyclo(Lys13−Asp17,Lys18−Asp22,Lys26−Asp30)[Ala1,Nle8,Lys18,Asp17,22,Leu27]hPTH(1−31)NH2 (9). Peptide 9 (EC50 = 0.14 nM) forces residues 13−30 into an extended helical conformation and is a more potent PTH receptor agonist than the parent linear hPTH(1−31)NH2 (1, EC50 = 4.7 nM). Comparative circular dichroism studies indicate that peptide 9 is highly helical even in the absence of TFE, indicating that residues 1−12 are also likely to be helical in the bioactive conformation. Taken together, these results provide strong structural evidence that hPTH binds to its receptor in an extended helical conformation.
All the virology on the web. http://www.virology.net (1999)

http://www.virology.net Thomas Valère PhD ‘All the Virology on the Web’ is a one-stop site for virology information on the internet. A prominent feature of the site is a comprehensive catalog of virus pictures on the web, ‘The Big Picture Book of Viruses’, which also functions as a resource for viral taxonomy. The catalog is searchable by virus, host, disease or genome family. Many images come with detailed information about the source of the image (site/e-mail address of author), related www sites, sequence data online and related literature. HSV, for example, comes with 19 pictures and many links (see screen capture). There are links to all the virology-related websites that might be of interest to virologists and others interested in learning more about viruses. The web contains enormous amounts of information and finding what you're looking for can be difficult. ‘All the Virology on the Web’ has attempted to help the user solve this problem by listing the sites in an organized manner. For further assistance, a search by keyword is also available. You will be amazed at what is available out there on the web for virologists. Check the site often as it is changing almost every day. The lists of virology-related sites are well organized and all are accessible from the home page and from the more descriptive complete table of contents. There are even links to virology dictionaries. Specific virus sites. A collection of websites that focus on an individual virus or viral family, from adenoviruses to togaviruses. AIDS and HIV. A very complete collection of AIDS and HIV sites on the web, organized by topic. Emerging viruses. Here you will find a collection of Ebola, Marburg and similar viral pathogens. Plant viruses. All of the plant virus websites have been conveniently collected in one central location and organized by topic. Organizations and groups. A collection of links to microbiology and virology departments and institutes, as well as virology laboratories, scientific societies and scientific companies. Graduate programs in virology. These are all the graduate school programs in the world that focus on virology and have a website to tell you about it. Online virology courses and tutorials. In addition to ATV's own virology courses and tutorials, there is a catalog of many other online educational resources. Viral vectors and gene therapy. This provides links to gene therapy related sites, universities, institutions and clinical trial sites. The site is updated almost daily and users are encouraged to suggest new sites not currently listed. Due to the popularity of this site, mirror sites have been established in the UK, China and Spain to provide faster access to users in Europe and Asia. The site has won numerous awards and deserves its name – All the Virology on the Web.
Cellular Gene Dose and Kinetics of Gene Expression in Mouse Livers Transfected by High-Volume Tail-Vein Injection of Naked DNA (2002)

Direct gene transfer to mammalian tissues has significant potential for biomedical research and gene therapy. Recently, the efficient transfer of naked plasmid DNA to the mouse liver by a rapid high-volume tail-vein injection was reported. We carried out a systematic analysis of the dose and time dependence of the expression of the Escherichia coli beta-galactosidase gene transferred by this technique. Surprisingly, the DNA concentration of the administered solution determined primarily the cellular gene dose and, hence, the expression of the transgene in individual hepatocytes, while the number of transfected cells was largely independent of the supplied plasmid mass. Transgene expression was transient: after a rapid onset and a peak at 8 h past injection, it gradually declined and was no longer detectable 4 weeks later. Although gene transfer was accompanied by tissue damage and subsequent regenerative proliferation, the decline in transgene expression was not due to increased hepatocyte turnover or to promoter downregulation, but instead cells apparently lost the plasmid DNA. Furthermore, we show that "nakedness" of the injected DNA is indeed a prerequisite for efficient transfer by the hydrodynamics-based procedure. Our data provide important clues for the successful use of this gene transfer technique, and may point directions for studies on the underlying mechanisms.
ChemInform Abstract: ELECTRONIC STRUCTURE AND NITROGEN HYBRIDIZATION IN β‐AMINOVINYLPHOSPHONIUM SALTS BY CARBON‐13 NUCLEAR MAGNETIC RESONANCE (1977)

Abstract Aus dem Propinyl‐triphenylphospho‐ nium‐bromid (I) entstehen mit Aminen (II) die Addukte (III), deren Hybridisierung untersucht wird.
A New 34-GHz 3.5-mm Low-Cost Utility Coaxial Connector Featuring Low Leakage, Low Standing-Wave Ratio, and Long Life (Short Papers) (1976)

A utility 3.5-mm connector was designed to cover frequencies above 18 GHz in coaxial transmission lines. The superior cost/performance characteristics of this new connector are presented.
ChemInform Abstract: 5′‐O‐Phosphonomethyl‐2′,3′‐dideoxynucleosides: Synthesis and Anti‐HIV Activity. (1991)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
A Novel Ferredoxin-Dependent Glutamate Synthase from the Hydrogen-Oxidizing Chemoautotrophic Bacterium<i>Hydrogenobacter thermophilus</i>TK-6 (2007)

Glutamate synthases are classified according to their specificities for electron donors. Ferredoxin-dependent glutamate synthases had been found only in plants and cyanobacteria, whereas many bacteria have NADPH-dependent glutamate synthases. In this study, Hydrogenobacter thermophilus, a hydrogen-oxidizing chemoautotrophic bacterium, was shown to possess a ferredoxin-dependent glutamate synthase like those of phototrophs. This is the first observation, to our knowledge, of a ferredoxin-dependent glutamate synthase in a nonphotosynthetic organism. The purified enzyme from H. thermophilus was shown to be a monomer of a 168-kDa polypeptide homologous to ferredoxin-dependent glutamate synthases from phototrophs. In contrast to known ferredoxin-dependent glutamate synthases, the H. thermophilus glutamate synthase exhibited glutaminase activity. Furthermore, this glutamate synthase did not react with a plant-type ferredoxin (Fd3 from this bacterium) containing a [2Fe-2S] cluster but did react with bacterial ferredoxins (Fd1 and Fd2 from this bacterium) containing [4Fe-4S] clusters. Interestingly, the H. thermophilus glutamate synthase was activated by some of the organic acids in the reductive tricarboxylic acid cycle, the central carbon metabolic pathway of this organism. This type of activation has not been reported for any other glutamate synthases, and this property may enable the control of nitrogen assimilation by carbon metabolism.
ChemInform Abstract: Highly Selective Palladium‐Catalyzed Synthesis of Protected α,β‐Unsaturated Methyl Ketones and 2‐Alkoxy‐1,3‐butadienes. High‐Speed Chemistry by Microwave Flash Heating. (2000)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Inhibition of Aurora-A results in increased cell death in 3-dimensional culture microenvironment, reduced migration and is associated with enhanced radiosensitivity in human nasopharyngeal carcinoma (2009)

Mitosis related Aurora-A kinase is amplified in a variety of carcinomas. Overexpression of Aurora-A contributes to tumorigenesis and disease progression, and has emerged as an attractive molecular target for the design of anticancer drugs. In this study, we investigated the function of Aurora-A selectively small molecule inhibitor VX-680 in nasopharyngeal carcinoma (NPC) CNE-2 cells. We found that VX-680 suppressed proliferation and induced apoptosis of 2Dimensional (2D) cultured NPC CNE-2 cells. Moreover, CNE-2 cells formed a tumor-like cell mass in 3Dimensional (3D) matrix culture microenvironment, and the tumor mass formation could be impaired when pretreated with VX-680 for indicated time. Similarly, when adding VX-680 to preformed 3D CNE-2 tumor mass, the tight spatial tumor mass experienced apparent apoptotic cell death and consequently dissociated into individual dead cells, as detected by cleaved Caspase-3 immunofluorescence assay. The migration assay showed that VX-680 decreased NPC CNE-2 cell migration ability in a dose-dependent manner. Our further study revealed that X-ray irradiation and VX-680 upregulated p53 expression level as well as arrested cell cycle in G(2)/M, sensitized NPC CNE-2 cells to radiation and effectively resulted in cell death. In summary, our data indicated that Aurora-A small molecule inhibitor VX-680, potently destructed tumor formation and induced apoptosis, reduced cell migration and enhanced radiosensitivity, offering a promising therapeutic agent for human NPC.
16β-METHYL CORTICAL STEROIDS (1958)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXT16β-METHYL CORTICAL STEROIDSD. Taub, R. D. Hoffsommer, H. L. Slates, and N. L. WendlerCite this: J. Am. Chem. Soc. 1958, 80, 16, 4435Publication Date (Print):August 1, 1958Publication History Published online1 May 2002Published inissue 1 August 1958https://pubs.acs.org/doi/10.1021/ja01549a095https://doi.org/10.1021/ja01549a095research-articleACS PublicationsRequest reuse permissionsArticle Views496Altmetric-Citations33LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Kindalike Cubism: Using Programmable Cameras for Photography in More Dimensions (2011)

We use a widely-available, programmable digital camera to create four-dimensional volumes of images that become part of an interactive art installation. The four dimensions we use are the two spatial dimensions of the camera image plane, plus focus and camera position. Analyzing the subject from multiple vantage points, we capture hierarchies of information about our subject. This visual data is then reconstituted into viewable twodimensional images from the four-dimensional volume. The resultant images bear a resemblance to cubist art in which the informational sum of the individual parts is greater than any single photograph.
Simple but novel test method for quantitatively comparing robot mapping algorithms using SLAM and dead reckoning (2013)

This article presents a comparative study between a well-known SLAM (Simultaneous Localization and Mapping) algorithm, called Gmapping, and a standard Dead-Reckoning algorithm; the study is based on experimental results of both approaches by using a commercial skid-based turning robot, P3DX. Five main base-case scenarios are conducted to evaluate and test the effectiveness of both algorithms. The results show that SLAM outperformed the Dead Reckoning in terms of map-making accuracy in all scenarios but one, since SLAM did not work well in a rapidly changing environment. Although the main conclusion about the excellence of SLAM is not surprising, the presented test method is valuable to professionals working in this area of mobile robots, as it is highly practical, and provides solid and valuable results. The novelty of this study lies in its simplicity. The simple but novel test method for quantitatively comparing robot mapping algorithms using SLAM and Dead Reckoning and some applications using autonomous robots are being patented by the authors in U.S. Patent Application Nos. 13/400,726 and 13/584,862.