gebhart/patrepeval huggingface dataset:
Reconstitution of nucleoprotein complexes with mammalian heterogeneous nuclear ribonucleoprotein (hnRNP) core proteins. (1983)

Newly transcribed heterogeneous nuclear RNA (hnRNA) in the eucaryote cell nucleus is bound by proteins, giving rise to large ribonucleoprotein (RNP) fibrils with an inherent substructure consisting largely of relatively homogeneous approximately 20-nm 30S particles, which contain core polypeptides of 34,000-38,000 mol wt. To determine whether this group of proteins was sufficient for the assembly of the native beaded nucleoprotein structure, we dissociated 30S hnRNP purified from mouse ascites cells into their component proteins and RNA by treatment with the ionic detergent sodium deoxycholate and then reconstituted this complex by addition of Triton X-100 to sequester the deoxycholate. Dissociation and reassembly were assayed by sucrose gradient centrifugation, monitoring UV absorbance, protein composition, and radiolabeled nucleic acid, and by electron microscopy. Endogenous RNA was digested and reassembly of RNP complexes carried out with equivalent amounts of exogenous RNA or single-stranded DNA. These complexes are composed exclusively of groups of n 30S subunits, as determined by sucrose gradient and electron microscope analysis, where n is the length of the added nucleic acid divided by the length of nucleic acid bound by one native 30S complex (about 1,000 nucleotides). When the nucleic acid: protein stoichiometry in the reconstitution mixture was varied, only complexes composed of 30S subunits were formed; excess protein or nucleic acid remained unbound. These results strongly suggest that core proteins determine the basic structural properties of 30S subunits and hence of hnRNP. In vitro construction of RNP complexes using model nucleic acid molecules should prove useful to the further study of the processing of mRNA.
Recognizing Partially Hidden Objects (1985)

In this paper, an approach is described for recognizing and locating partially hidden objects in an image. In the approach, templates are formed from the edge contours of the objects sought. Segments of each template are matched to segments of the edge contours of the image. A Bayesian approach is used to decide the probability an object has been located given that matches occur.
Stomach Lesions of Gray Diarrhea in Mink (1974)

Hcmatologic atad patltologic examination on thirty-live mink, clinically affectedwith gray diarrhea, were carried otut. Four had other recognizable diseases. Twenty-two1?ad microscopic stomach lesions, not described in other mink diseases. The lesions weremultifocal ancl consisted of loss of the ntiddle and basal layers c>f the mucosa, with neutro-phil and monont?c1ear cell infiltratiort. Fibrosis was observecl in the stomach lesions ofsome. Dircct ancl indirect fluorescent antibody tecltniques, tttilizing sera from affectedmink, failed to demonstrate an aul-oantibocly in them. Hematologic responses of aflecteclmink varied widely.
Exploiting FPGAs from Higher Level Languages A Signal Analysis Case Study (2017)

Field Programmable Gate Arrays (FPGAs) are usually perceived as difficult to exploit due to the High Level of expertise required to program them. In the last years, the major FPGAs vendors have produced different High Level Synthesis (HLS) tools to help programmers during the flow of acceleration of their algorithms through the hardware architecture. However, these tools often use languages considered low level from the point of view of data scientists and are still much too difficult to use for software developers. This complexity limits their usage in a number of fields, from data science to signal processing, where the computational power offered by FPGAs could be highly beneficial. One way to overcome this problem is to realize libraries of widely used algorithms that transparently offload the computation to the FPGAs device from modern High Level Languages. Our work presents an interface between R, a language commonly used by statisticians and data scientists, and an FPGA connected via PCI-Express (PCIe). We use the Reusable Integration Framework for FPGA Accelerators (RIFFA) to send and receive data from PCIe connection. To showcase the use of the described interface and the improvements given by making use of FPGAs in signal analysis applications we used Xilinx Vivado Design Suite to implement an accelerated and optimized version of the Autocorrelation Function (ACF) present in the default libraries used by R.
LAN/MAN interconnection to ATM: a simulation study (1992)

The authors compare three strategies, namely, reservation, on-the-fly, and hybrid, for the support of connectionless internet traffic on asynchronous transfer mode (ATM). The comparison is based on a simulation experiment in which a mix of virtual paths (some carrying connectionless traffic, some carrying connection-oriented traffic) are multiplexed on a 150 Mb/s trunk, called the bottleneck trunk. Results based on a variety of measures (cell and burst loss rates, cell delay, and burst delay) show that the on-the-fly approach, which aggressively exploits unused bandwidth, is superior to the reservation scheme, which is handicapped by the renegotiation overhead and the conservative usage of bandwidth. The hybrid scheme exhibits a performance behavior between the other two. This is a bit disappointing, considering the fact that the hybrid scheme is expected to be consistently better than both schemes. Still the hybrid scheme offers some interesting features (e.g. lower delays for the background traffic) which should be further explored by a more extensive examination of the working parameters.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Observation of radical intermediates for unusual carbon-nitrogen bond formation of .alpha.-aminomalonate to give an .alpha.-diamine linkage. ESR studies on electron transfer reactions in cobalt(III)-polyamine complexes (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTObservation of radical intermediates for unusual carbon-nitrogen bond formation of .alpha.-aminomalonate to give an .alpha.-diamine linkage. ESR studies on electron transfer reactions in cobalt(III)-polyamine complexesTakahiko Kojima, Junichi Tsuchiya, Satoru Nakashima, Hiroaki Ohya-Nishiguchi, Shigenobu Yano, and Masanobu HidaiCite this: Inorg. Chem. 1992, 31, 12, 2333–2340Publication Date (Print):June 1, 1992Publication History Published online1 May 2002Published inissue 1 June 1992https://pubs.acs.org/doi/10.1021/ic00038a009https://doi.org/10.1021/ic00038a009research-articleACS PublicationsRequest reuse permissionsArticle Views220Altmetric-Citations9LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
The adductor ratio: a new tool for joint line reconstruction in revision TKA (2014)

II.
Reflectron velocity map ion imaging (2005)

We report the first demonstration of velocity map ion imaging in a reflectron configuration. Under these conditions, the velocity-resolving capabilities associated with velocity map ion imaging are achieved simultaneously with long flight times and the high mass resolution characteristic of reflectron time-of-flight mass spectrometers. The photodissociation of OCS at 230 nm has been used to characterize the velocity-focusing performance of the instrument. The reflectron geometry provides an increased magnification factor over conventional velocity map ion imaging.
Computational inference of replication and transcription activator regulator activity in herpesvirus from gene expression data (2008)

One of the main aims of system biology is to understand the structure and dynamics of genomic systems. A computational approach, facilitated by new technologies for high-throughput quantitative experimental data, is put forward to investigate the regulatory system of dynamic interaction among genes in Kaposi's sarcoma-associated herpesvirus network after induction of lytic replication. A reconstruction of transcription factor activity and gene-regulatory kinetics using data from a time-course microarray experiment is proposed. The computational approach uses nonlinear differential equations. In particular, the quantitative Michaelis-Menten model of gene-regulatory kinetics is extended to allow for post-transcriptional modifications and synergic interactions between target genes and the Rta transcription factor. The kinetic method is developed within a Bayesian inferential framework using Markov chain Monte Carlo. The profile of the Rta transcriptional regulator, other post-transcriptional regulatory genes and gene-specific kinetic parameters are inferred from the gene expression data of the target genes. The method described here provides an example of a principled approach to handle a wide range of transcriptional network architectures and regulatory activation mechanisms to reconstruct the activity of several transcription factors and activation kinetic parameters in a single regulatory network.
The <i>Limulus</i> sperm motility‐initiating peptide initiates acrosome reactions in sea water lacking potassium (1985)

During fertilization in Limulus, the spermatozoa first attach to the egg and then undergo an acrosomal reaction. In this reaction, the acrosomal vesicle exocytoses, and a long, preformed acrosomal filament is extruded (and subsequently penetrates the egg chorion). The egg surface component that triggers the acrosome reaction has not yet been solubilized; therefore, previous studies have examined either spontaneous acrosome reactions or acrosome reactions that were triggered by eggs (or insoluble egg fragments), elevated extracellular Ca2+, or Ca2+ ionophores. In this study, we report a new method for initiating acrosome reactions in Limulus sperm. When the Limulus sperm motility-initiating peptide (SMI) is added to sperm in K+-free sea water, greater than 90% acrosome reactions are initiated within 5 min. However, less than 5% acrosome reactions occur either in K+-free sea water lacking SMI or when SMI is added to sperm in either normal sea water or K+- and Ca2+-free sea water. Experiments with K+ ionophores (nigericin and valinomycin), a K+ channel blocking agent (tetraethyl ammonium), an Na+ ionophore (monensin), and reagents that increase the intracellular pH (monensin, nigericin, and NH4Cl) indicate that changes in intracellular K+, Na+, or H+ do not mediate SMI-initiated acrosome reactions. The K+/Ca2+ ratio determines whether or not SMI will initiate acrosome reactions, with greater than 50% acrosome reactions being initiated when this ratio is below 0.3. In that K+ movement does not appear to be the critical event, possibly the K+/Ca2+ ratio either determines the rate of Ca2+ entry or controls the conformation of sperm surface molecules to allow SMI to initiate acrosome reactions in low K+.
1M1624 High Resolution Electron Tomography Analysis of the Cytoplasmic Dynein and Microtubule Complex(Molecular motor 2,The 49th Annual Meeting of the Biophysical Society of Japan) (2011)

Towards the construction of nano-transporter powcrcd by molecular motoT protein, we created a hybrid-nanomachine (DNA-ti]e-kinesin) using two- dimensional (2D) DNA nano-stmcture (DNA ti]e) as a scaffold and kinesin as the functional module.High-speed AFM imaging of DNA-tite-kinesin, censtructed by mixing 20 nt Cy5-ssDNA ]abeled kinesin (DNA-kinesin) with Cy3-labled DNA origami tile having 1 to 3 bindjng sites (20 nt capture ssDNA
The role of form cues in motion processing (2011)

The visual system initially collects spatially localised estimates of motion and then needs to interpret these local estimates to generate 2D object motion and self-motion descriptions. Commonly sinusoidal gratings have been employed to study the perception of motion and while these stimuli are useful for investigating the properties of spatial- and temporal-frequency tuned detectors they are limited. They remove textural and shape cues that are usually present in natural images, which has led to models of motion processing that ignore those cues. However, the addition of texture and shape information can dramatically alter perceived motion direction. Recent work has shown that orientation-tuned simple cells are stimulated by moving patterns because of their extended temporal integration. This response (sometimes called motion streaks) allows orientation-tuned detectors to contribute to motion perception by signalling the axis of motion. The orientation cue is influential even if second-order streaks which could not have been produced by image smear are employed. This suggests that any orientation cue may be used to determine local direction estimates: a view that is extended to argue that aperture shape itself may have an impact by providing orientation cues which are incorporated into the direction estimation process. Oriented textural cues will also be shown to distort direction estimates, even though current models suggest they should not. The conclusion is that pattern information has a critical role in early motion processing and should be incorporated more systematically into models of human direction perception.
Coherent spin-qubit photon coupling (2017)

Electron spins hold great promise for quantum computation due to their long coherence times. An approach to realize interactions between distant spin-qubits is to use photons as carriers of quantum information. We demonstrate strong coupling between single microwave photons in a NbTiN high impedance cavity and a three-electron spin-qubit in a GaAs triple quantum dot. We resolve the vacuum Rabi mode splitting with a coupling strength of $g/2π\simeq31$ MHz and a qubit decoherence of $γ_2/2π\simeq 20$ MHz. We can tune the decoherence electrostatically and obtain a minimal $γ_2/2π\simeq 10$ MHz for $g/2π\simeq 23$ MHz. The dependence of the qubit-photon coupling strength on the tunable electric dipole moment of the qubit is measured directly using the ac Stark effect. Our demonstration of strong spin-photon interaction is an important step towards coherent long-distance coupling of spin-qubits.
Cytochrome c oxidase models: a dinuclear iron(III) porphyrin-copper(II) complex with a sulfur bridge (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCytochrome c oxidase models: a dinuclear iron(III) porphyrin-copper(II) complex with a sulfur bridgeBarbara R. Serr, Christine E. L. Headford, Oren P. Anderson, C. Michael Elliott, Kevork Spartalian, Vladimir E. Fainzilberg, William E. Hatfield, Brian R. Rohrs, Sandra S. Eaton, and Gareth R. EatonCite this: Inorg. Chem. 1992, 31, 26, 5450–5465Publication Date (Print):December 1, 1992Publication History Published online1 May 2002Published inissue 1 December 1992https://pubs.acs.org/doi/10.1021/ic00052a022https://doi.org/10.1021/ic00052a022research-articleACS PublicationsRequest reuse permissionsArticle Views226Altmetric-Citations26LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Kinetics of hydrogen reduction of magnetite concentrate particles at 1623–1873 K relevant to flash ironmaking (2020)

The reduction of magnetite concentrate particles by H2 has been investigated in drop-tube reactor (DTR) to obtain a rate equation that can be applied to the design and analysis of an ironmaking flash reactor. The temperature range in this work is 1623 to 1873 K, where the magnetite concentrate particles become molten droplets. Full reduction of the magnetite concentrate is achieved at a residence time greater than 4 s and H2 partial pressure of 0.15 atm within the temperature range investigated. Computational fluid dynamics analysis is used to improve the accuracy of the obtained rate expressions where the variation of particle temperature is taken into consideration. The reduction of magnetite concentrate particles in this temperature range is best described by the nucleation and growth model with an Avrami parameter n = 1 and a first-order dependence on the partial pressure of H2. The activation energy of reaction is 180 kJ mol−1.
Validation of the MedGem Device for Measurement of Resting Metabolic Rate (2004)

1708 Resting metabolic rate (RMR) is the largest component of total daily energy expenditure. Using prediction equations to estimate this variable is often inaccurate and unreliable, leading to potentially large errors in recommendations for energy balance. Actual measurement of RMR by indirect calorimetry (IC) improves accuracy and precision, but requires costly and cumbersome equipment. Recently, a novel, inexpensive, handheld device for RMR measurement (MedGem [MG], HealtheTech, Golden, CO) has become available, but its ability to accurately measure RMR in comparison with commonly used clinical IC instruments has not been reported. PURPOSE: To validate the MG against two clinical IC instruments, the Deltatrac (DT, Datex-Ohmeda, Helsinki, FN) and the Vmax (Viasys Healthcare, Yorba Linda, CA) and compare these results with Douglas Bag (DB) measurements. METHODS: Fiftyfour adults (29 women, 24 men) aged 20 to 50 completed the study. They averaged (± SD) 32 ± 11 yr with BMI of 26 ± 5 kg × m−2. After an overnight fast and 30 minutes in a reclined position, subjects were measured sequentially with MG, DT, Vmax, and DB in random and counterbalanced order. The Weir equation was used throughout with an adjustment for the urinary nitrogen based on an assumption of protein intake representing 16% total Kcal × d−;1. RESULTS: Mean ± SD RMR (Kcal × d−;1) for MG, DT, Vmax, and DB were 1494 ± 252, 1484 ± 247, 1452 ± 265, and 1518 ± 246 respectively. Mean differences in RMR between the MG and DT, Vmax, and DB, respectively were 10, 42, and 24 Kcal × d−;1. The intraclass correlation coefficient among the four instruments was 0.978 (95% CI = 0.966 to 0.986) indicating a high degree of overall agreement. Bland-Altman plots of differences among the six pairwise comparisons for instruments were very similar, revealing no patterns of accuracy bias through the range of RMR from about 1100 to 2100 Kcal × day−;1. CONCLUSIONS: The MG compares very well (high intraclass correlation) with two clinical IC instruments and DB for measurement of RMR. Thus, MG is a valid instrument, capable of accurate measurements of RMR. Supported in part by a grant from HealtheTech Corp., Golden CO.
The Antigen-Antibody Reaction (1952)

Summary Sodium uptake by the specific antigen-antibody precipitate was studied. In precipitin tubes containing an initial mole ratio of total sodium present to antibody in the precipitate of about 7:1, the mole ratio decreased after centrifugation and decanting the precipitate to about 0.2:1. After washing the precipitate three times the mole ratio had decreased further, to 0.03:1. It is concluded that the residual activity was due to sodium adsorption by the glass of the tubes and that under the particular experimental conditions no sodium-ions were taken up by the antigen-antibody precipitate.
Prospects of Using Silicon Photomultipliers for the Astroparticle Physics Experiments EUSO and MAGIC (2005)

We discuss the silicon photomultiplier (SiPM) as a novel photon detector for the astroparticle experiments MAGIC and EUSO. For these experiments we pursue two different developments of the SiPM. One development is pursued at MEPhI, where prototypes are available, and we will describe some characteristics. The second development, which is using the back illumination principle is at present in its design phase.
MINUTES OF CORROSION MEETING, OCTOBER 21, 1946 (1956)

From introduction: "This meeting was called by Mr. S. H. Smiley in order to review progress made on the corrosion problem since the last meeting on September 5th, and to devise a definite program on the specific problem of the corrosion of the coolant coolers."
Novel technique for MOS pulse capacitance measurements in C(<i>V</i>) and C(<i>t</i>) mode (1976)

For the purpose of MOS doping profile evaluation and generation time constant analysis a pulse capacitance measurement setup has been developed. The principle of operation consists in the capacitance of MOS varactors tuning the resonant frequency of a parallel resonant circuit. The range of capacitance measurement is 0–1000 pF, corresponding to the MHz range. First the pulse voltage ranges from 0 to ±100 V, and second the resolution of time ranges from 1 msec to 100 sec. So MOS-doping profiles as well as transients of capacitance are available. The accuracy of capacitance measurements is better than 0.1%. Output of data is done by punched tape for subsequent evaluation on a desk calculator.
Transpor table Imam Reoording Med i u~ ProPOsal of ISAC base Save and Carrr) Srsten (1990)

In contrast to PACS. an on-line system, we consider an off-line system called ISAC (Image Save and Carry), and. discuss its necessity and possibility of practical use in the medical field. The basic idea of ISAC system is to save or file both images and characters in a portable recording medium and to transport a huge amount of digital data in an off-line way. Because the compatibility is most important and inevitable for common and wide use, we focus on the standardization of 110 devices. We also propose the combined system of PACS and ISAC, and discuss its advantages.
Reproducibility and Scalability of Microwave-Assisted Reactions (2011)

Microwave Heating 138 of processing versatility, safety, reaction monitoring and optimization. Importantly, this processing method also avoids the limitations associated with the design of scaled microwave cavities, including the associated costs. In this chapter we intend to highlight the most important reports studying and solving problems concerning the reproducibility and scalability of microwave-assisted processes, from the early reactions performed in unmodified domestic ovens to the recent syntheses utilizing dedicated apparatus or suitable flow instrumentation.