gebhart/patrepeval huggingface dataset:
Structural features of Argonaute–GW182 protein interactions (2013)

MicroRNAs (miRNAs) guide Argonaute (Ago) proteins to target mRNAs, leading to gene silencing. However, Ago proteins are not the actual mediators of gene silencing but interact with a member of the GW182 protein family (also known as GW proteins), which coordinates all downstream steps in gene silencing. GW proteins contain an N-terminal Ago-binding domain that is characterized by multiple GW repeats and a C-terminal silencing domain with several globular domains. Within the Ago-binding domain, Trp residues mediate the direct interaction with the Ago protein. Here, we have characterized the interaction of Ago proteins with GW proteins in molecular detail. Using biochemical and NMR experiments, we show that only a subset of Trp residues engage in Ago interactions. The Trp residues are located in intrinsically disordered regions, where flanking residues mediate additional weak interactions, that might explain the importance of specific tryptophans. Using cross-linking followed by mass spectrometry, we map the GW protein interactions with Ago2, which allows for structural modeling of Ago-GW182 interaction. Our data further indicate that the Ago-GW protein interaction might be a two-step process involving the sequential binding of two tryptophans separated by a spacer with a minimal length of 10 aa.
The effect of rosemary Extract and cold plasma treatments on bacterial community diversity in poultry ground meats (2019)

To provide safer food, many technologies have been used to preserve food. One such technology is cold plasma, which can reduce viable bacterial counts in various food matrices. However, bacterial communities in food matrices before and after cold plasma treatment have not been investigated. In this communication, the EcoPlates™ were used to physiologically profile bacterial communities from poultry ground meat treated with rosemary, cold plasma or both. The cultures in the plates were incubated at 25 °C for seven days in an OmniLog® system. Responses of the bacterial communities to 31 chemicals were measured on formazan production. The results show that the three parameters of the Gompertz growth curves were observed in all samples, 2-hydroxybenzoic acid could not be used, while pyruvic acid methyl ester was used for a carbon source by the bacterial communities from all meat samples, each bacterial community metabolized different numbers of chemical compounds at different rates, and reduction of bacterial functional diversity was observed in the poultry meat samples treated with cold plasma and rosemary. In the future, investigations on whether the physiological profiling in bacterial communities be used as an indicator for effectiveness of cold plasma treatment of meat samples.
ChemInform Abstract: SYNTH. 5‐SUBSTITUIERTER 1‐(1‐ADAMANTYL)‐TETRAZOLE UND VERWANDTER VERBINDUNGEN (1970)

Abstract Aus dem Adamantyl‐chlorkohlensäureester (I) erhält man in Gegenwart von Acetonitril über (II) neben dem Chlorid (III) durch Reaktion mit dem Acetonitril Folgeprodukte von (IV).
Ulnar Collateral Ligament Repair With Collagen-Dipped FiberTape Augmentation in Overhead-Throwing Athletes (2019)

UCL repair with internal brace augmentation is a viable option for amateur overhead throwers with selected UCL injuries who wish to return to sport in a shorter time frame than allowed by traditional UCL reconstruction.
Metal complexes of nonamethylimidodiphosphoramide (1968)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMetal complexes of nonamethylimidodiphosphoramideKent P. Lannert and Melvin D. JoestenCite this: Inorg. Chem. 1968, 7, 10, 2048–2051Publication Date (Print):October 1, 1968Publication History Published online1 May 2002Published inissue 1 October 1968https://pubs.acs.org/doi/10.1021/ic50068a018https://doi.org/10.1021/ic50068a018research-articleACS PublicationsRequest reuse permissionsArticle Views69Altmetric-Citations15LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
23 SUPEROXIDE ANION IS NECESSARY FOR TUMOR NECROSIS FACTOR α--INDUCED MIP-2 EXPRESSION (2005)

<h3></h3> Macrophages are involved in the pathogenesis of immune and inflammatory disorders of the lung. Tumor necrosis factor (TNF)-α is a proinflammatory cytokine that has been linked to both injury and repair in the acute and chronic lung diseases. TNF-α also generates reactive oxygen species, including superoxide anion. We have previously shown that reactive oxygen species (ROS) generation is important in macrophage cytokine gene expression, so we hypothesized that superoxide anion generation was necessary for MIP-2 expression after TNF-α stimulation. Using real time PCR, we found that TNF-α increased MIP-2 expression greater than 25-fold, and cells exposed to diphenyleneiodonium (DPI), a non-specific inhibitor of NADPH oxidase, decreased expression to control levels. To assure ourselves that the effect of DPI was due to decreased superoxide anion, we performed similar experiments with apocynin, a specific NADPH oxidase inhibitor, and L-NMMA, an inhibitor of nitric oxide synthase. We found that apocynin inhibited MIP-2 expression approximately 50%, and L-NMMA had no significant effect in macrophages stimulated with TNF-α. To further confirm our hypothesis, we over expressed Cu,Zn-SOD and found that MIP-2 expression was reduced to near control levels when compared to cells expressing an empty vector. These findings show that MIP-2 gene expression in TNF-α-stimulated macrophages is dependent on adequate generation of superoxide anion and is only partially due to NADPH activity. VA Merit Review.
Male reproductive fitness and queen polyandry are linked to variation in the supergene <i>Gp-9</i> in the fire ant <i>Solenopsis invicta</i> (2012)

Supergenes are clusters of tightly linked loci maintained in specific allelic combinations to facilitate co-segregation of genes governing adaptive phenotypes. In species where strong selection potentially operates at different levels (e.g. eusocial Hymenoptera), positive selection acting within a population to maintain specific allelic combinations in supergenes may have unexpected consequences for some individuals, including the preservation of disadvantageous traits. The nuclear gene Gp-9 in the invasive fire ant Solenopsis invicta is part of a non-recombining, polymorphic supergene region associated with polymorphism in social organization as well as traits affecting physiology, fecundity and behaviour. We show that both male reproductive success and facultative polyandry in queens have a simple genetic basis and are dependent on male Gp-9 genotype. Gp-9(b) males are unable to maintain exclusive reproductive control over their mates such that queens mated to Gp-9(b) males remain highly receptive to remating. Queens mated to multiple Gp-9(B) males are rare. This difference appears to be independent of mating plug production in fertile males of each Gp-9 genotype. However, Gp-9(b) males have significantly lower sperm counts than Gp-9(B) males, which could be a cue to females to seek additional mates. Despite the reduced fitness of Gp-9(b) males, polygyne worker-induced selective mortality of sexuals lacking b-like alleles coupled with the overall success of the polygyne social form act to maintain the Gp-9(b) allele within nature. Our findings highlight how strong worker-induced selection acting to maintain the Gp-9(b) allele in the polygyne social form may simultaneously result in reduced reproductive fitness for individual sexual offspring.
Low resolution NMR spectroscopy: a tool to study protein denaturation: I. Application to diamagnetic whey proteins (1989)

Summary A method using low resolution NMR spectroscopy is described for investigating whey protein thermal denaturation. The method is based on measuring at 20 °C changes in water proton transverse (T 2 ) relaxation parameter following the denaturing treatment. This parameter is shown to be sensitive to protein denaturation and not to other phenomena such as gelation. Examples are given for the qualitative study of protein thermal denaturation in whey protein concentratc, β-lactoglobulin, α-lactalbumin, bovine serum albumin and immunoglobulins aqueous solutions and for the quantitative determination of thermal denaturation in whey protein concentrate solutions.
Improved fluorescence staining technique for estimating turnover of the human stratum corneum (2006)

The replacement time of the human stratum corneum of fifteen body regions was determined by measuring the days required for a strongly substantive fluorescent marker, Dansyl chloride, to disappear. Tbe horny layer was stained by a 24-h patch exposure to 5% Dansyl chloride (5-dimethylamino- 1-naphthalene-sulphonyl chloride) in petrolatum. In most body regions the renewal time for the horny layer was about 2 weeks, in agreement with the findings of other investigators. The advantage of Dansyl chloride over another compound, tetrachlorosalicylanilide, which has been used for this purpose, is that it is not an irritant.
Transport properties of substituted poly(acetylenes) (1987)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTransport properties of substituted poly(acetylenes)Mario Leclerc and Robert E. Prud'hommeCite this: Macromolecules 1987, 20, 9, 2153–2159Publication Date (Print):September 1, 1987Publication History Published online1 May 2002Published inissue 1 September 1987https://pubs.acs.org/doi/10.1021/ma00175a018https://doi.org/10.1021/ma00175a018research-articleACS PublicationsRequest reuse permissionsArticle Views116Altmetric-Citations32LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
THE CONVERSION OF PROGESTERONE INTO TESTOSTERONE BY THE MOUSE OVARY AND ITS RELATIONSHIP TO ADRENAL X ZONE DEGENERATION (1963)

SUMMARY Using incubation techniques, with progesterone as precursor, the capacity of the mouse ovary to form steroids was examined. Ovaries were taken from three groups of mice: (1) immature mice, (2) mature virgin females, (3) primigravid animals. The formation of the following steroids was suggested by the analytical methods used: 16-oxooestrone, 17- epi oestriol, 17α-hydroxyprogesterone, 20α-hydroxyprogesterone, androstenedione, testosterone. Yields were very small from the ovaries of immature mice. The yields of oestrogens were greatest from ovaries of mature virgin females and of testosterone from those of pregnant mice. The factors contributing to the disappearance of the X zone of the adrenal gland during first pregnancy are discussed. It is concluded that one major factor is the secretion of testosterone by the ovary of the gravid mouse.
Experience with the VHDL environment (2003)

The authors present their work in the use of the VHDL (VHSIC Hardware Development Language) environment on three different models of DEC VAX (1, 4, and 5.8 Mips (million instruction per second)). The work included the development of a number of VHDL models and sequences of input stimuli and the gathering of performance data from their execution. Even though the set of benchmarks is not in any way exhaustive, they represent typical applications, making it possible to derive performance models for predicting both time and memory requirements for VHDL modeling and simulation. It is shown that the performances of the VHDL environment on the three computers correlate perfectly with their Mips figures; thus the performance models developed can be normalized to a 1-Mips VAX machine.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Scaling the Resistance Vessels (1991)

Classifying members of the biosphere has been a major problem for biologists and remains so.1 It is still with a sense of awe that we look at the classification proposed by Linnaeus in his Systema Naturae in 1735, which was adopted by botanists until the ultimate establishment of the system founded by Jussieu in 1789. All plants were divided by Linnaeus into 24 classes: The first 20 were based on the number or arrangement of the stamens; the next three classes included plants having monoecieus, diecieus, and polygammodiecieus flowers, respectively; and the last dass included all known cryptogams. These classes were then divided into orders, based on characters of the gynoecium or the fruit. The system became known as the "artificial system," because it gave no due to the relation-ship of species or genera, but merely afforded a key to their rapid determination. Other artificial systems had been proposed before Linnaeus, but none was so extensively adopted. Even today with sophisticated category and hierarchy theories we are unable to state clearly the bounds of a biological species.KeywordsMean Arterial PressureArterial TreeResistance VesselAllometric ScalingParallel PathThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
Transmission electron microscopy of epitaxial gallium arsenide grown on a variety of silicon substrates by metallorganic chemical vapor deposition (1987)

The growth of GaAs layers on silicon substrates is under extensive investigation with a view to achieving the integration of GaAs-based optoelectronic devices with Si integrated circuit technology. The large lattice mismatch between Si and GaAs (-4%) together with the differences in the thermal expansion coefficients between the two materials results in a highly stressed interface. Several different approaches have been undertaken in attempts to reduce the dislocation density of the GaAs layer. The inclusion of graded composition GaAsP ‘buffer’ layers, intermediate Ge layers and the inclusion of strained layer superlattices in the growth regime have been reported by many workers. Growth of GaAs directly on Si has been reported to yield GaAs heteroepitaxial films suitable for electronic applications such as FETs and low threshold AlGaAs/GaAs double heterostructure injection lasers.
A Device for Gated Autosynchronous Luminescence Detection (2011)

The sensitive detection of delayed luminescence (τ > 100 μs) can be achieved with an optomechanical instrument, the gated autosynchronous luminescence detector (GALD). The device effectively combines an excitation chopper, a detection chopper, and a dichroic mirror into a single element. With dimensions of 12 mm (H), 32 mm (W), and 90 mm (L), it is designed for insertion into the differential interference contrast (DIC) prism slot of a BX51 Olympus microscope. The GALD described here employed a compact high-power UV LED as the excitation source to capture images of Giardia lamblia cysts indirectly labeled with a europium chelate/streptavidin conjugate. Labeled cells were clearly visible in the complete absence of autofluorescence and signal intensity was sufficient to capture high-resolution color images within several seconds. Shorter exposure intervals of 100 ms on a monochrome Andor iXON camera delivered time-gated luminescence images with a signal-to-noise ratio better than 114:1.
One Dimensional Modeling and Experimental Validation of Single Cylinder Pneumatic Combustion Hybrid Engine (2011)

International audience
Filtering by Aliasing (2013)

In this manuscript we describe a fundamentally novel approach to the design of anti-aliasing filters. The approach, termed Filtering by Aliasing, incorporates the frequency-domain aliasing operation itself into the filtering task. The spectral content is spread with a periodic mixer and weighted with a simple analog filter before it aliases at the sampler. By designing the system according to the formulations presented in this manuscript, the sampled output will have been subjected to sharp, highly programmable anti-alias filtering. This manuscript describes the proposed Filtering by Aliasing idea, the effective programmable anti-aliasing filter, its design, and its range of frequency responses. The manuscript also addresses the implementation sensitivities of the proposed Filtering by Aliasing approach and provides a performance comparison against existing techniques in the context of reconfigurable anti-alias filtering.
Multiresolution lossless compression scheme (2002)

A multiresolution lossless image compression scheme based on several new tools will be presented in this paper. An improved multiresolution Haar transform will be developed, combined with pre- and post-processing and with a new entropy coder, to give the overall compression scheme. This research is mainly useful for image compression in the medical imaging area, where changes between original and decompressed images are to be avoided.
X-ray induced chemical reaction revealed by in-situ X-ray diffraction and scanning X-ray microscopy in 15 nm resolution(Conference Presentation) (2016)

The detection sensitivity of synchrotron-based X-ray techniques has been largely improved due to the ever increasing source brightness, which have significantly advanced ex-situ and in-situ research for energy materials, such as lithium-ion batteries. However, the strong beam-matter interaction arisen from the high beam flux can significantly modify the material structure. The parasitic beam-induced effect inevitably interferes with the intrinsic material property, which brings difficulties in interpreting experimental results, and therefore requires comprehensive evaluation. Here we present a quantitative in-situ study of the beam-effect on one electrode material Ag2VO2PO4 using four different X-ray probes with different radiation dose rate. The material system we reported exhibits interesting and reversible radiation-induced thermal and chemical reactions, which was further evaluated under electron microscopy to illustrate the underlying mechanism. The work we presented here will provide a guideline in using synchrotron X-rays to distinguish the materials’ intrinsic behavior from extrinsic structure changed induced by X-rays, especially in the case of in-situ and operando study where the materials are under external field of either temperature or electric field.
Time and Memory (2002)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Neurology HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Neurology journal
Charging damage during residual metal overetching (1999)

The influence of electron and ion temperatures on charging damage during residual metal (latent antenna) overetching in high-density plasmas is investigated by Monte Carlo simulations. The tunneling current through a thin gate oxide, electrically connected to the antenna, increases significantly with electron temperature, mainly as a result of changes in plasma current and ion energy distribution. However, the current decreases with ion temperature as ion shading: (a) directly decreases the ion flux to the antenna and (b) neutralizes the negative charge at the upper mask sidewalls, thus allowing more electrons to enter the pattern. The role of exposed antenna areas (trench bottoms and perimeter) is examined from the perspective of current imbalance.
CaF2 overlayers to preserve the ideal termination of Sb/GaAs(110) (1994)

Photoemission spectroscopy has been performed on CaF2 overlayers on as cleaved (‘‘bare’’) GaAs(110) and GaAs(110) terminated with 1 monolayer of Sb. We observe Fermi-level positions of 0.7 and 0.9 eV above the valence-band maximum on n type for the bare and Sb terminated surfaces, respectively. On p type, we observe close to flatbands for both surfaces. These Fermi-level positions are 0.45 eV on n type and 0.8 eV on p type, below previous reports for CaF2 on the bare surface. We obtain positions close to the previous results by exposing the sample to a flux of UV photons. Upward Fermi-level movement is observed on n and p type for both surfaces. Several complimentary experiments will be discussed which indicate that the observed Fermi-level movement is not due to surface charging. We present a possible interpretation of the data which involves a low density of interface states created by the CaF2deposition and the formation of F centers when the sample is exposed to UV radiation. Analysis of core-level line shapes suggests the presence of Ga–F bonding for CaF2 on the bare surface. We present results which indicate that the ideal Sb termination is preserved for CaF2 on Sb terminated GaAs and evidence of CaF2–Sb bonding will be discussed.