gebhart/patrepeval huggingface dataset:
A highly sensitive immunofluorometric assay for the measurement of aldosterone in small sample volumes: validation in mouse serum (2007)

Data on the involvement of aldosterone in the regulation of the renin-angiotensin-aldosterone system (RAAS) in rodents are still scarce, partly due to the high sample volumes needed by commercially available assays and to the very low aldosterone concentrations present. We have developed a highly sensitive and non-isotopic immunoassay, requiring a volume of only 50 microl serum for a duplicate measurement, employing a highly specific monoclonal antibody against aldosterone. The assay was validated in human and mouse samples and exhibited a linear working range from 10 to 1000 pg/ml. Values obtained after a chromatographic purification step correlated significantly to the dichloromethane extraction ordinarily used. Basal aldosterone values were measured in 75 mouse hybrids and found within the linear range (173+/-21 pg/ml), with no significant difference between males and females. Additionally, we show an increase in serum aldosterone in mice from 3 to 11 weeks of age. Mice of the same genetic background were treated with dexamethasone intraperitoneally (n=7), resulting in significantly decreased concentrations (35+/-3 vs 114+/-33 pg/ml in controls; P<0.001). In contrast, adrenocorticotropic hormone resulted in significantly increased serum aldosterone (603+/-119 pg/ml; n=7; P<0.001), as did the physiological stimulation of the RAAS by a high K(+)/low Na(+) diet (1369+/-703 vs 172+/-36 pg/ml). In conclusion, we have developed and validated an extremely sensitive assay for determination of aldosterone concentrations from very small serum samples, which could be especially useful in pharmacological intervention studies in rodent models.
Synthesis, Structure, and Ferromagnetic Behavior of Decacopper(II) Cluster Complex Supported by Hexaanionic <i>p</i>-<i>tert-</i>Butylthiacalix[6]arene (2002)

A nanosized decacopper(II) cluster complex was synthesized using p-tert-butylthiacalix[6]arene as a ligand. In the complex, the calixarene assumes a "pinched cone" conformation and acts as an undecadentate ligand via six phenoxy oxygen and five sulfur atoms. The resulting pentacopper(II) subunits are connected by two hydroxo and two oxo groups to form decacappoer(II) cluster core. The core structure remains intact in alcoholic solution as shown by ESI MS. The temperature dependence of chimT clearly shows ferromagnetic behavior of the core due to orthogonality between two adjoining magnetic orbitals of copper(II) ions.
Molecular Orientation of Langmuir−Blodgett Films of Designed Heme Protein and Lipoprotein Maquettes (1998)

De novo designed tetra-α-helical heme proteins (αssα)2, comprising two identical helix-loop-helix subunits, αssα, were immobilized as monolayer films on solid substrate using the Langmuir−Blodgett (LB) technique. These LB heme protein films were characterized by circular dichroism (CD), ultraviolet−visible (UV−vis), and Fourier transformed infrared (FTIR) spectroscopy. During the formation of monolayer films on substrate, the (αssα)2 heme proteins dissociate into their αssα subunits, but remain α-helical and retain the bis-histidine heme ligation. The α-helices are oriented in the film close to parallel to the substrate plane and the heme macrocycle planes are tilted at 40°. There is no detectable ordering of the molecules in the plane of the monolayer. However, when the loop region was palmitoylated to confer amphiphilic character to the heme proteins, a profound change of state at high surface pressure was displayed. Transfer of this high-pressure film onto solid substrate also generates a film with α-helices near 0° and hemes planes at 40°, but with added order: remarkably the α-helices and one heme edge assume a strong orientation parallel to the direction of withdrawal of the substrate from the LB subphase.
94. The chemistry of the triterpenes. Part XV. The environment of the unreactive double bond of polyporenic acid A (1953)

T. G. Halsall, E. R. H. Jones and A. J. Lemin, J. Chem. Soc., 1953, 468 DOI: 10.1039/JR9530000468
Cell therapy with EBV-specific cytotoxic T-lymphocytes for patients with nasopharingeal carcinoma. (2010)

2509 Background: Nasopharyngeal carcinoma (NPC) is an Epstein-Barr virus (EBV)-related malignancy expressing a restricted set of viral antigens. The outcome of patients with NPC failing conventional radiochemotherapy is poor, the median overall survival of patients receiving second line treatments being less than two years. Hence, there is a need for alternative therapies capable of improving survival and associated with reduced toxicity. Methods: Since 2001, we have implemented a T-cell therapy program for patients with NPC failing conventional treatment. We have treated 27 patients with disease resistant/relapsing after >2 lines of radiochemotherapy in two sequential trials of cell therapy with autologous EBV-specific cytotoxic T-lymphocytes (CTL). In detail, EBV CTL (4 escalating doses to a maximum of 8×107 CTL/dose in the first trial, or 2 doses of 3-4 × 108 CTL in the second trial) were administered in patients without or with preceding lymphoablative chemotherapy and followed by low-dose recombinant human interleukin-2 (rhIL-2). The proportion of patients achieving response or stable disease lasting at least 3 months (RECIST criteria), as well as immunologic correlates of effective treatment, were evaluated. Results: Overall, the objective control of disease was 57%, with 6/27 patients showing complete (n=1), partial (n=4), and minimal (n=1) responses, and 10/27 disease stabilization (median duration 7 months). No severe adverse events were observed after cell therapy; 4 patients showed an inflammatory reaction at the tumor site. The use of preparative chemotherapy and increased CTL dose did not influence outcome. Importantly, patients showing response to cell therapy showed the emergence of EBV LMP2 antigen-specific T-cells in their peripheral blood. Conclusions: EBV-specific CTL therapy is safe and associated with clinical benefit in patients with advanced NPC refractory to standard therapies. The use of CTL with higher specificity for the EBV subdominant antigens expressed by the tumor, such as LMP2, at an earlier stage of disease, could further implement the strategy and ameliorate the outcome of patients with relapsing/refractory NPC. No significant financial relationships to disclose.
Nanometer-scale two-terminal semiconductor memory operating at room temperature (2005)

Based on a nanometer-scale semiconductor channel with an intentionally broken geometric symmetry, we have realized a type of memory device that consists of only two terminals, rather than the minimum of three terminals in conventional semiconductor memories. The charge retention time is at least 10 h at cryogenic temperatures and a few minutes at room temperature. Furthermore, the simplicity of the design allows the active part of the devices to be made in a single nanolithography step which, along with the planar structure of the device, provides promising possibilities for a high integration density. (C) 2005 American Institute of Physics.
Transport and Percolation in a Low-Density High-Mobility Two-Dimensional Hole System (2007)

We present a study of the temperature and density dependence of the resistivity of an extremely high quality two-dimensional hole system grown on the (100) surface of GaAs. For high densities in the metallic regime (p > or approximately4x10;{9} cm;{-2}), the nonmonotonic temperature dependence ( approximately 50-300 mK) of the resistivity is consistent with temperature dependent screening of residual impurities. At a fixed temperature of T=50 mK, the conductivity versus density data indicate an inhomogeneity driven percolation-type transition to an insulating state at a critical density of 3.8x10;{9} cm;{-2}.
Impact Ionisation on Planar GaAs Gunn Diodes: Analysis and Reduction by Anode Desigb (2012)

No abstract available.
Experimental Studies on the Performance of Slipper Bearings Within Axial-Piston Pumps (2004)

The objectives of this study are to experimentally investigate the performance characteristics of similar slipper bearings using different socket geometries. In this study, the lubrication equations for the bearing are derived based upon an assumption that the bearing deformations are small and that they may be modeled linearly. This study is based upon the hypothesis that variations in the socket geometry will impact the deformation characteristics of the bearing and thereby have an effect on the overall performance of the design. To test this hypothesis, bearings with different socket geometries are designed and tested and compared to the original bearing design with standard socket geometry. The experimental results are then used with the analytical results to numerically infer the minimum fluid-film thickness and the magnitude of deformation for the bearing. Conclusions are drawn from these results which indicate that socket geometry has a significant impact on the bearing performance and that both leakage and load-carrying capacity may be altered by adjusting the location of the contact point within the ball-and-socket joint relative to the center of the ball.
Measurements of intermodal sensitivity of a two-mode holey fiber to strain, temperature, and hydrostatic pressure (2013)

The intermodal sensitivity of a two-mode birefringent holey fiber to strain, temperature and hydrostatic pressure is measured in the spectral domain. In an experimental setup comprising a broadband source, a polarizer, a twomode birefringent holey fiber under a variable physical parameter, an analyzer and a compact spectrometer, the spectral interferograms are resolved. These are characterized by the equalization wavelength at which spectral interference fringes have the largest period due to the zero group optical path difference between the fundamental, the LP<sub>01</sub> mode and the higher-order, the LP<sub>11</sub> mode. The spectral interferograms are processed to retrieve the phase as a function of the wavelength. From the retrieved phase functions corresponding to different values of the physical parameter, the intermodal sensitivity as a function of wavelength is obtained. Using this approach, the intermodal sensitivity to strain, temperature and hydrostatic pressure is measured for two orthogonal (<i>x</i> and <i>y</i>) polarizations.
Syntheses and Reactions of Functional Polymers (1971)

N-メタクリロイル-2-オキサゾリドンを2-オキサゾリドンのNa塩とメタクリル酸クロニドから合成した。このモノマー (M1) のラジカル重合, アニオン重合を行ない, さらにスチレン (M2) と共重合させ, モノマー反応性比r1=0.27, r2=1.10 (70℃) を得た。またこのモノマーのQ, e値をそれぞれ0.49, 0.32と評価した。さらにラジカル重合で得られたポリマーのタクティシティをNMRスペクトルで決定した。このポリマーはジメチルホルムアミド, ジメチルスルホキシドには溶解するが, 一般の有機溶媒には不溶である。さらにこのポリマーによるフェノール, 核酸塩基, ヌクレオシドの吸着を検討した。
Elastic FWI for VTI media: A synthetic parameterization study (2016)

A major challenge for multiparameter full-waveform inversion (FWI) is the inherent trade-offs (or cross-talk) between model parameters. Here, we perform FWI of multicomponent data generated for a synthetic VTI (transversely isotropic with a vertical symmetry axis) model based on a geologic section of the Valhall field. A horizontal displacement source, which excites intensive shear waves in the conventional offset range, helps provide more accurate updates to the SV-wave vertical velocity. We test three model parameterizations, which exhibit different radiation patterns and, therefore, create different parameter trade-offs. The results show that the choice of parameterization for FWI depends on the availability of long-offset data, the quality of the initial model for the anisotropy coefficients, and the parameter that needs to be resolved with the highest accuracy. Presentation Date: Monday, October 17, 2016 Start Time: 2:15:00 PM Location: 166 Presentation Type: ORAL
Detection of allergens in plantain (<i>Plantago lanceolata</i>) pollen (2000)

Three specific allergens (of 17, 19, and 40 kDa, respectively) have been detected in plantain pollen. Further studies on a larger number of patients will determine whether these proteins may be considered major allergens. Cross-reactivity between grass and plantain pollen is mainly caused by a 30-kDa protein in plantain pollen. Group 5 grass-pollen allergen is probably responsible for most grass/plantain cross-reactivity.
LDL cholesterol lowering by bile acid malabsorption during inhibited synthesis and absorption of cholesterol in hypercholesterolemic coronary subjects. (2002)

The massive reduction in LDL and increase in HDL-C obtained using our triple therapy suggests that the combination of stanol ester with only moderate doses of statin and resin makes it possible to control LDL-C levels effectively in hypercholesterolemic subjects.
Soliton solutions for a perturbed nonlinear Schrodinger equation (1993)

Using the inverse scattering transform the authors found one-parameter and the breather-like four-parameter soliton solutions of a perturbed nonlinear Schrodinger equation which describes the pulse propagation in optical fibres in the femtosecond regime
Performance analysis and laser linewidth requirements for optical PSK heterodyne communications systems (1986)

An optical PSK heterodyne communications receiver is investigated. The receiver is based on the decision-directed phase-locked loop. The performance of the phase-locked loop subsystem is analyzed taking into account both shot noise and laser phase noise. It is shown that for reliable phase locking (rms phase error less than 10°), heterodyne second-order loops require at least 6771 electrons/s per volt every hertz of the laser linewidth. This number corresponds to the limit when the loop dumping factor η is infinitely large; if <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">\eta = 0.7</tex> , then the loop needs 10 157 electrons/(s . Hz). If the detector has a unity quantum efficiency and <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">\lambda = 1.5 \mu</tex> m, the above quoted numberers give 0.9 pW/ kHz for <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">\eta \rightarrow \infty</tex> and 1.35 pW/kHz for <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">\nu = 0.7</tex> . The loop bandwidth required is also evaluated and found to be <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">155 \Delta\nu</tex> , where <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">\Delta\nu</tex> is the laser linewidth. Finally, the linewidth permitted for PSK heterodyne recievers is evaluated and found to be <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2.26 \cdot 10^{-3} R_{b}</tex> where R <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">b</inf> is the system bit rate. For <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">R_{b}=100</tex> Mbit/s, this leads to <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">\Delta\nu < 226</tex> kHz. Such and better linewidths have been demonstrated with laboratory external cavity lasers. For comparison, ASK and FSK heterodyne receivers are much more tolerant to phase noise,-they can tolerate <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">\Delta\nu</tex> up to 0.09 R <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">b</inf> . At the same time, homodyne receivers impose much more stringent requirements on the laser linewidth ( <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">\Delta\nu < 3 \cdot 10^{4} R_{b}</tex> ).
Analysis of Host Range Phenotypes of Primate Hepadnaviruses by In Vitro Infections of Hepatitis D Virus Pseudotypes (2004)

Hepatitis B virus (HBV) and woolly monkey hepatitis B virus (WMHBV) have natural host ranges that are limited to closely related species. The barrier for infection of primates seems to be at the adsorption and/or entry steps of the viral replication cycle, since a human hepatoma cell line is permissive for HBV and WMHBV replication following transfection of cloned DNA. We hypothesized that the HBV and WMHBV envelope proteins contain the principal viral determinants of host range. As previously shown by using the hepatitis D virus (HDV) system, recombinant HBV-HDV particles were infectious in chimpanzee as well as human hepatocytes. We extended the HDV system to include HDV particles pseudotyped with the WMHBV envelope. In agreement with the natural host ranges of HBV and WMHBV, in vitro infections demonstrated that HBV-HDV and WM-HDV particles preferentially infected human and spider monkey cells, respectively. Previous studies have implicated the pre-S1 region of the large (L) envelope protein in receptor binding and host range; therefore, recombinant HDV particles were pseudotyped with the hepadnaviral envelopes containing chimeric L proteins with the first 40 amino acids from the pre-S1 domain exchanged between HBV and WMHBV. Surprisingly, addition of the human amino terminus to the WMHBV L protein increased infectivity on spider monkey hepatocytes but did not increase infectivity for human hepatocytes. Based upon these data, we discuss the possibility that the L protein may be comprised of two domains that affect infectivity and that sequences downstream of residue 40 may influence host range and receptor binding or entry.
Wind Loads and Building Response Predictions Using Force Balance Techniques (1986)

The use of force balance techniques to measure mean and dynamic wind loads on models of buildings and structures is discussed. Advantages of using force balance techniques over estimating wind loads from the integration of mean surface pressures or aeroelastic tests and limitations associated with the inherent use of approximate mode shapes and the omission of aeroelastic effects are discussed. Advanced features, such as mode shape corrections and coupled lateral-torsional response estimates are presented. Second generation force balance techniques are outlined.
Open circuit TSD method and anomalous air gap current in Teflon FEP (1985)

The paper shows an extension in the technique of thermally stimulated discharge in open circuit (TSD) consisting in simultaneously measuring the current and its charge on the external circuit as a function of temperature. With it, we were able to show that the noise usually appearing in the current curve of negatively charged Teflon FEP is due to microbreakdown events in the sample. Moreover, we detected the ejection of positive charges out the surface of virgin uncharged FEP samples, when a positive bias voltage was applied in the measuring circuit.
Detecting small, low-contrast moving targets in infrared video produced by inconsistent sensor with bad pixels (2015)

This paper addresses the problem of finding small and low-contrast moving targets in infrared (IR) video sequences produced by sensors with inconsistent parameters, such as intensity offset and gain as well as bad pixels. This sensor variability makes it difficult to apply methods based on frame registration using simple pixel differences. Our proposed algorithm uses regression to normalize the variations of intensity offset and gain between compared registered frames. A statistical criterion is used to calculate the threshold for the difference between normalized intensities of two frames. The algorithm for finding the differences between frames is also used to create a bad pixel mask either on- or offline. This mask is essential for the reduction of false detection rates. Our experiments show that this approach produces good results and can be used for detection of small, low-contrast targets in high dynamic range IR data. The proposed algorithm also produces good results for detecting moving targets in cases when objects are occluded by sparse vegetation. © 2015 Society of Photo-Optical
ChemInform Abstract: C‐NUCLEOSIDE STUDIES. PART 10. A NEW SYNTHESIS OF 3‐(2,3,5‐TRI‐O‐BENZYL‐β‐D‐RIBOFURANOSYL)PYRAZOLE AND ITS CONVERSION INTO 4‐NITRO‐3(5)‐β‐D‐RIBOFURANOSYLPYRAZOLE (1981)

Abstract Das Produkt (IIIa) der Umsetzung der Komponenten (I) und (II) liefert bei der Tosylierung ein Gemisch der Isomeren (IVa)/(IVb) mit dem (IIIa)‐Tosylat (IIIb).
Isolation amplifier for high voltage measurement using a resonant control loop (2005)

Some papers describe a novel pulse width modulation, which makes enter converter's current control loop in a resonant mode and permits an accurate tracking of the reference. Here, we investigate an analog high voltage measurement board, which implements an isolation amplifier using this modulation strategy. Thus, the isolation amplifier involves an analog-to-digital converter whose operating mode depends on this modulation technique. Then, a linear model of the encoder-decoder is found. The results demonstrate its excellent performances
Modeling of Radical Copolymerization near a Selectively Adsorbing Surface:  Design of Gradient Copolymers with Long-Range Correlations (2005)

A new approach to synthesis of copolymers with long-range correlations is proposed. Using Monte Carlo simulations and the lattice bond-fluctuation model, we perform the computer-aided sequence design of a two-letter (AB) copolymer with quenched primary structure near a chemically homogeneous impenetrable surface. We simulate an irreversible radical copolymerization of selectively adsorbed A and B monomers with different affinity to the surface, allowing for a strong short-range monomer− (A−) surface attraction. To describe the chain growth analytically, we introduce and investigate a simple theoretical model based on stochastic processes and probabilistic statistics. We find that this model provides a close approximation to the simulation data and explains a number of statistical properties of copolymer sequences. It is shown that, under certain conditions, the chain propagation near the adsorbing surface proceeds as a randomly alternating growth, leading to a copolymer with a specific quasi-gradient primary structure and power-law long-range correlations in distribution of different monomer units along the chain. The gradient extends along the entire chain for any chain length. We find that the statistical properties of the copolymer sequences correspond to those of a one-dimensional fractal object with scale-invariant correlations. Thus, just by radical copolymerization of two monomers with different affinity to a certain plane surface, it is possible to obtain copolymers with a gradient primary structure.
Engineered Low Temperature Hydrothermal Synthesis of Phase-Pure Lead-Based Perovskites Using Ethylenediamine Tetra-acetic Acid Complexation (2002)

Thermodynamic modeling of hydrothermal solutions was used to examine the influence of ethylenediamine tetra-acetic acid (EDTA) on the synthesis of lead-based perovskites, PbTiO3 and PbZr0.52Ti0.48O3. Temperature, pH, input reagent concentrations, and Pb/(Ti + (Zr)) and Pb/EDTA ratios were examined. Thermodynamic calculations from 25 to 100 °C indicated that, when the Pb/EDTA and Pb/(Ti + (Zr)) molar ratios were 2.0 and Pb input concentration was 0.1 m, phase-pure perovskites formed across the greatest pH range (7−14+). Precipitation of unwanted unary lead phases such as PbO was suppressed in all cases. In the absence of EDTA and solutions containing a molar excess of lead ions, the formation of phase-pure perovskites was limited to narrow pH ranges (5−8 and above 14) because of PbO precipitation. Theoretical predictions were experimentally validated with use of X-ray diffraction, transmission electron microscopy, energy dispersive spectrometry, and atomic absorption spectroscopy. Experimental results validated the theoretical predictions at temperatures above a minimum reaction temperature. Relative to comparable hydrothermal systems free of EDTA, the addition of EDTA was found to lower the minimum reaction temperature by 70 °C for PbTiO3 (70 °C) and 25 °C for PbZr0.52Ti0.48O3 (125 °C).