gebhart/patrepeval huggingface dataset:
Chromosomal replication origin fro harveyi functions in Escherichia coli: (initiation/high copy lethal (HCL) genes/gene-polypeptide relationsi (2016)

The chromosomal replication origin (oriC) of Vibrio harveyi has been isolated on a plasmid and shown to func- tion as an origin in Escherichia coli. The nucleotide sequence of the V. harveyi oriC was determined. From a comparison of this se- quence with oriC sequences of five enteric bacteria, we derived a consensus sequence of bacterial origins that function in E. coli. This consensus sequence identifies 122 positions within-oriC where nucleotide substitutions can occur without loss of origin function. These positions are clustered rather than scattered. Four inter- related nine-base-pair repeats and eight of the dam methylation G-A-T-C sites are conserved in the consensus sequence. Very few relative insertion-deletion changes occur, and these are localized to one region of oriC. The genes for three polypeptides linked to the V. harveyi oriC were identified by using in vitro protein syn- thesis directed by deletion derivative plasmid templates. One of these genes, coding for a 58,000 Mr polypeptide and located 3.0 kilobase pairs from the V. harveyi oriC region, is lethal to E. coli when many copies (approximately 40 per cell) are present (high copy lethal or HCL gene). In addition, nucleotide sequence anal- ysis showed that a different gene, the gid gene to the left of oriC, is highly conserved between E. coli and V. harveyi, whereas the coding region to the right of oriC is much less conserved.
Lateral <i>p</i>-<i>n</i> junctions and quantum wires formed by quasi two-dimensional electron and hole systems at corrugated GaAs/AlGaAs interfaces (1992)

We report the results of modeling lateral p-n junctions and p-n-p quantum wire structures at corrugated GaAs/AlGaAs interfaces, using the surface orientation dependent amphoteric nature of Si doping. We determine the potential landscape and the electron and hole charge densities within a semiclassical Thomas–Fermi screening model, and then solve the two-dimensional Schrödinger equation using finite elements for the quantized electron and hole states at the heterointerfaces. We demonstrate the formation of a one-dimensional electron system confined between two lateral p-n junctions, and discuss the advantages of this structure compared to conventional electrostatic confinement schemes for fabricating quantum wires.
Commercial refrigeration with ammonia and CO2 as working fluids. Discussion (1998)

Un programme de RD 2/ le CO 2 comme fluide basse temperature dans une installation en cascade; 3/ une installation commerciale utilisant le CO 2 comme fluide unique. Des equipements au CO 2 , concus pour des installations frigorifiques, ne sont pas encore disponibles sur le marche. Il est encore necessaire d'apporter des innovations, afin notamment de tirer profit des avantages tels que la dimension, le cout et l'efficacite. Le programme a debute par des mesures en laboratoire, et on a construit plusieurs installations frigorifiques commerciales utilisant l'ammoniac et du CO 2 comme fluide de transfert de chaleur volatil. L'article presente quelques resultats experimentaux; on evalue plusieurs combinaisons possibles d'ammoniac et de CO 2 .
Metabolism of 1,25-dihydroxycholecalciferol in the rat (1972)

Administration of 60 pmoles of 1,25-dihydroxycholecalciferol to vitamin D-deficient rats on a low calcium diet gives a maximal intestinal calcium transport response in 7 hr and a maximal bone calcium mobilization response in 12 hr. During the 48 hr after injection of radioactive 1,25-dihydroxycholecalciferol, unchanged 1,25-dihydroxycholecalciferol accounts for 71-98% of the radioactivity found in the intestine with minor amounts appearing in more polar metabolites. In the bone, for the 1st 12 hr, 1,25-dihydroxycholecalciferol is the major form (75-82%) present while at 24 hr, the amount of 1,25-dihydroxycholecalciferol decreases with a corresponding rise in the amounts of metabolites both less polar and more polar than the 1,25-dihydroxycholecalciferol. Since these metabolies are at their highest concentration when bone calcium mobilization is decreasing, they are most likely not responsible for the calcium mobilization observed during the 1st 12 hr. The appearance of water-soluble radioactivity in the kidney, plasma, liver, and muscle 24 hr after 1,25-dihydroxycholecalciferol injection has been demonstrated. The present results suggest that, although 1,25-dihydroxycholecalciferol is converted to further metabolites in the rat, it is probably the form of vitamin D responsible for initiating intestinal calcium transport and bone calcium mobilization.
A Volumetric Dispenser for Insect Media (1967)

Journal Article A Volumetric Dispenser for Insect Media Get access Edwin L. Soderstrom, Edwin L. Soderstrom Stored-Product Insects Research Branch. Market Quality Research Division, Agr. Res. Serv., USDA, Fresno, California Search for other works by this author on: Oxford Academic PubMed Google Scholar Anthony E. Lovitt Anthony E. Lovitt Stored-Product Insects Research Branch. Market Quality Research Division, Agr. Res. Serv., USDA, Fresno, California Search for other works by this author on: Oxford Academic PubMed Google Scholar Journal of Economic Entomology, Volume 60, Issue 4, 1 August 1967, Pages 1179–1180, https://doi.org/10.1093/jee/60.4.1179 Published: 01 August 1967 Article history Accepted: 16 March 1967 Published: 01 August 1967
The Microaerophile <i>SPirillum volutans</i> : Cultivation on Complex Liquid and Solid Media (1982)

Spirillum volutans grows only under microaerobic conditions in a peptone-succinate-salts broth, but can grow aerobically when the peptone is replaced by vitamin-free acid-hydrolyzed casein broth. The addition of potassium metabisulfite, norepinephrine, catalase or superoxide dismutase (SOD) permitted aerobic growth in peptone-succinate-salts broth. A combination of catalase and SOD had a synergistic effect. S. volutans lacked catalase and had only a low level of peroxidase activity, but did possess SOD activity (12 to 14 U/mg of protein). The organism was found to be extraordinarily sensitive to exogenous hydrogen peroxide. Illumination of peptone-succinate-salts broth generated hydrogen peroxide and rendered the medium inhibitory to growth. A combination of catalase and SOD prevented this inhibition. Growth of S. volutans on solid media, not previously possible, was accomplished by the use of vitamin-free acid-hydrolyzed casein and peptone-succinate-salts agar media; maximum growth responses were dependent on the following combination of factors: addition of bisulfite, catalase, or SOD, protection of the media from illumination, incubation in a highly humid atmosphere, and incubation under atmospheres of 12% oxygen or less. The results indicate that the microaerophilic nature of S. volutans is attributable largely to the high sensitivity of the organism to exogenous hydrogen peroxide and, to a lesser extent, superoxide radicals occurring in the culture medium.
Analysis of safety requirements in the context of system faults and human errors (2002)

This paper describes an approach to the incremental and iterative analysis of requirements for safety-critical systems. Safety strategies are developed to ensure that hazard states are avoided and the system's integrity is maintained in the presence of failures in the system components. The approach supports an understanding of human error and the formulation of human error tolerance requirements ensuring that safety is preserved in the presence of user errors. The approach is illustrated with an example from the aerospace industry.
47,XXX (1989)

LINDEN, MARY G.; BENDER, BRUCE G.; HARMON, ROBERT J.; MRAZEK, DAVID A.; ROBINSON, ARTHUR Author Information
Development of Models for Predicting the Distribution of Indoor Nitrogen Dioxide Concentrations (1989)

Extensive data on residential indoor and outdoor NO2 levels have been collected in a limited number of U.S. locations. To date, researchers have analyzed these data sets individually, but have not analyzed them in the aggregate. Results have not, therefore, been suitable for application in a nationwide exposure assessment. This paper presents an analysis of indoor and outdoor NO2 field measurements from five U.S. metropolitan areas for homes with gas-fueled ranges and discusses potential applications of the results. Using linear regression analysis, the relationship between indoor NO2 and various predictor variables was explored. Results indicated that ambient NO2 levels alone explain an estimated 37 percent of the variability in indoor NO2 levels, that the relationship between indoor and outdoor NO2 concentrations differs significantly from summer to winter months, and that homes with range pilot lights have indoor levels approximately 7 ppb greater than homes without pilot lights. A logistic regression model which predicts the distribution of indoor NO2 levels based on ambient NO2 concentrations was developed. Estimation and testing of the logistic model indicated good model performance. The model is particularly useful for addressing policy-oriented questions that involve the concept of "acceptable" threshold levels for human exposure to NO2.
THE REMOVAL OF CARTILAGE MATRIX, IN VIVO, BY PAPAIN (1958)

The intravenous injection of crystalline papain into young rabbits results in depletion of cartilage matrix throughout the body, with loss of rigidity and collapse of the ears, provided the enzyme is inactivated by oxidation or sulfhydryl blocking agents prior to administration. Cysteine-activated crystalline papain, when injected intravenously, produces little or no change in cartilage. The changes which occur in cartilage following an injection of inactivated crystalline papain are indistinguishable from those produced by crude papain. Activation of crude papain by cysteine prior to injection results in loss of its capacity to produce in vivo changes in cartilage. The progressive changes which take place in cartilage in vivo also occur in vitro in isolated rabbit ears removed shortly after an injection of crude papain or inactivated crystalline papain. In vitro ear collapse occurs rapidly at 37 degrees C. and does not occur at 4 degrees C. Collapse is enhanced by exposing the cartilage to cysteine and prevented by exposure to iodoacetamide or p-chloromercuribenzoate. The direct action of crystalline papain on plates of normal cartilage, in vitro, results in the same gross and histological changes which were observed in vivo. The direct action is accelerated by cysteine and inhibited by iodoacetamide or p-chloromercuribenzoate. The intravenous injection of iodoacetamide-treated bromelin produces the same in vivo changes in cartilage as papain. Untreated bromelin has no demonstrable effect on cartilage. It is suggested that the reason for the failure of activated papain to enter cartilage, after being injected intravenously, is that it probably reacts with a substrate or substrates in the blood. Oxidized or otherwise inactivated papain, in contrast, is readily taken up by cartilage and there converted to its active form.
Characterization by physisorption of a new class of microporous adsorbents: pillared clays (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCharacterization by physisorption of a new class of microporous adsorbents: pillared claysM. Safdar Baksh, Eustathios S. Kikkinides, and Ralph T. YangCite this: Ind. Eng. Chem. Res. 1992, 31, 9, 2181–2189Publication Date (Print):September 1, 1992Publication History Published online1 May 2002Published inissue 1 September 1992https://pubs.acs.org/doi/10.1021/ie00009a016https://doi.org/10.1021/ie00009a016research-articleACS PublicationsRequest reuse permissionsArticle Views298Altmetric-Citations77LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
The next Millennium (1999)

It is appropriate to reflect on the achievements in wound care in recent years and to speculate on its future direction in the year 2000 and beyond. A wide variety of wound treatments has always been used – some sensible, some dangerous – mostly designed to care for or ‘dress’ wounds. There has been little scientific interest in understanding how best to achieve healing. The belief that dressing wounds is the only consideration in wound care is a major barrier to achieving acceptance by professional colleagues that wound healing is a subject worthy of clinical and academic endeavour.
Suspended stripline over mushroom-like shorted metallic patches and its application to the dual-frequency dual-polarization antenna (2006)

This paper introduces a suspended stripline over mushroom-like SMPs that has excellent band stop filter characteristics, and apply it to the conventional DFDP antenna in order to enhance isolation. Since the structure of the suspended stripline over the SMP provides strong coupling between the feedline and the SMP, a significant skirt rejection band and near 0 dB the insertion loss outside the rejection band can be achieved with only two SMP unit cells
Vulvovaginal Chronic Graft-Versus-Host Disease With Allogeneic Hematopoietic Stem Cell Transplantation (2007)

III.
Protein kinase Cβ as a therapeutic target stabilizing blood–brain barrier disruption in experimental autoimmune encephalomyelitis (2013)

Disruption of the blood-brain barrier (BBB) is a hallmark of acute inflammatory lesions in multiple sclerosis (MS) and its animal model experimental autoimmune encephalomyelitis. This disruption may precede and facilitate the infiltration of encephalitogenic T cells. The signaling events that lead to this BBB disruption are incompletely understood but appear to involve dysregulation of tight-junction proteins such as claudins. Pharmacological interventions aiming at stabilizing the BBB in MS might have therapeutic potential. Here, we show that the orally available small molecule LY-317615, a synthetic bisindolylmaleimide and inhibitor of protein kinase Cβ, which is clinically under investigation for the treatment of cancer, suppresses the transmigration of activated T cells through an inflamed endothelial cell barrier, where it leads to the induction of the tight-junction molecules zona occludens-1, claudin 3, and claudin 5 and other pathways critically involved in transendothelial leukocyte migration. Treatment of mice with ongoing experimental autoimmune encephalomyelitis with LY-317615 ameliorates inflammation, demyelination, axonal damage, and clinical symptoms. Although LY-317615 dose-dependently suppresses T-cell proliferation and cytokine production independent of antigen specificity, its therapeutic effect is abrogated in a mouse model requiring pertussis toxin. This abrogation indicates that the anti-inflammatory and clinical efficacy is mainly mediated by stabilization of the BBB, thus suppressing the transmigration of encephalitogenic T cells. Collectively, our data suggest the involvement of endothelial protein kinase Cβ in stabilizing the BBB in autoimmune neuroinflammation and imply a therapeutic potential of BBB-targeting agents such as LY-317615 as therapeutic approaches for MS.
Hormone Therapy in Cancer (1978)

As remarked earlier, the first endocrine procedure for the treatment of cancer was carried out in 1895 by Sir George Beatson. He performed oophorectomy in women with breast cancer and noted regression of metastatic deposits. The next advance came in 1941 when Huggins used oestrogens or orchidectomy to treat prostatic cancer. With the development of synthetic steroids it is not surprising that the role of hormones in the treatment of cancer has expanded considerably in recent years. Hormones are now used not only for treatment purposes but form an integral part of the overall management of the cancer patient. Recent advances in the understanding of the molecular role of hormones and the nature of receptors has encouraged a fresh look at hormones and cancer (see p. 74).
A Comparative Analysis for Office Investment Markets in South-East Asia (2004)

Most of the research undertaken into the determinants of office investment markets has examined European and North America cities. Although South-East Asia has been marked by high economic growth from the early 1990s to the mid-1997, it has largely been ignored. With the increasing globalisation of investments, it is very important to understand the environment of these investment markets. Before undertaking quantitative analysis for South-East Asia, it is also important to classify those markets in terms of the progress of their development. Opportunities in both the user and investor markets will be constrained by the legal system of property rights and the quality of professional advice available to those transacting property investments, with obvious implications for property rights. Therefore, in order to understand fully the property market, it is necessary to consider the nature and evolution of the markets as well as their economic condition. Analysts usually look at economic conditions at a national or regional level, as well as the urban economies. However, the cultural framework is usually ignored. Most of the popular analytical framework is justified by the demand and supply conditions in three sub-markets: user, investment and development. The first half of this paper will identify the macro-economic drivers of office rental values in South-east Asian cities over the period 1988 ñ 2001 by adopting the demand and supply framework. The paper uses the existing single-equation method to examine influences on office rents in five South-east Asian cities (Singapore, Hong Kong, Taipei, Kuala Lumpur and Bangkok). Real GDP, unemployment rate, floor space for office buildings, interest rate, lending rate, consumer index and service sector output will be included in examining the movements of office rental values. The second part of the paper will identify the importance and working of a range of institutional factors, and their status in each of these cities. Looking at these institutional factors in a particular circumstance may provide additional information on how the market works. This in turn, will help investors in their decision making process.
Comparison of the impact of cardiovascular guidelines on a working population. (2006)

For the two High Blood Pressure Management CPGs, the selected patients are not distributed the same way in the two CPGs. Observed agreement is poor between the two hypertension CPG versions. Screening people for specific health care should be based on risk calculation not on few variables. Feeding back our results to the CPG creator group should reduce the variability of recommendations.
Infrared tunable sub-100 fs pulses of gigawatt peak power at 1 kHz by parametric conversion of CPA Ti:sapphire laser radiation (1994)

High-energy femtosecond pulses tunable in a wide spectral region of the infrared are strongly needed for a variety of applications in nonlinear optics and spectroscopy. Optical parametric conversion has been demonstrated to be one of the most effective techniques for the generation of tunable ultrashort pulses due to the recent achievements in solid-state pump lasers and highly nonlinear crystals. In the case of low-energy femtosec­ond pulses synchronously pumped optical parametric oscillators have been extensively investigated. 1,2 For the generation of high-energy tunable pulses, a traveling wave parametric conversion seems to be the most favorable technique. 3,4
UBIQUINONE-10 AS AN ANTIOXIDANT (2008)

ABSTRACT Ubiquinol, the reduced form of coenzyme Q, is known as a lipid antioxidant. Its fully oxidized form, ubiquinone, would theoretically not have this activity. However, we report that ubiquinone-10, the oxidized form of coenzyme Q, demonstrated antioxidant activity in model studies against a lipid-soluble free radical generator, 2,2′-azobis(2,4-dimethylvaleronitrile). This was demonstrated at both 1:100 and 1:1,000 ratios of ubiquinone-10 to lipid (for both methyl linoleate and methyl linolenate). Ubiquinone-10 should therefore not be discounted as a possible antioxidant in more complex systems such as food. PRACTICAL APPLICATIONS Coenzyme Q (ubiquinol/ubiquinone) is an important component of electron transport in biological tissues and is also classified as a potent antioxidant. It is generally believed that the form that is active as an antioxidant is the reduced ubiquinol. In evaluating the loss of antioxidants in mackerel light and dark muscle tissues, it was observed that the oxidized form, ubiquinone, comprised over 87% of the total coenzyme Q in light muscle and over 93% in dark muscle and this observation did not change under anaerobic conditions. This study was done to determine if the oxidized form of coenzyme Q, ubiquinone, was capable of acting as an antioxidant. In this study, it is shown that it can function in this manner.
Studies in Steroid Total Synthesis. I. Resolution of a Bicyclic Intermediate (1954)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStudies in Steroid Total Synthesis. I. Resolution of a Bicyclic IntermediateA. John Speziale, John A. Stephens, and Quentin E. ThompsonCite this: J. Am. Chem. Soc. 1954, 76, 20, 5011–5013Publication Date (Print):October 1, 1954Publication History Published online1 May 2002Published inissue 1 October 1954https://pubs.acs.org/doi/10.1021/ja01649a002https://doi.org/10.1021/ja01649a002research-articleACS PublicationsRequest reuse permissionsArticle Views167Altmetric-Citations12LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts