gebhart/patrepeval huggingface dataset:
No Advantage of Cell-Penetrating Peptides over Receptor-Specific Antibodies in Targeting Antigen to Human Dendritic Cells for Cross-Presentation (2008)

Induction of CTL responses by dendritic cell (DC)-based vaccines requires efficient DC-loading strategies for class I Ags. Coupling Ags to cell-penetrating peptides (CPPs) or receptor-specific Abs improves Ag loading of DCs. In contrast to CPPs, receptor-specific Abs deliver conjugated Ags to DCs with high specificity, which is advantageous for in vivo strategies. It has, however, been speculated that CPPs facilitate uptake and endosomal escape of conjugated Ags, which would potently enhance cross-presentation. In this study, we directly compare the in vitro targeting efficiency of a humanized D1 Ab directed against the human DC surface receptor DC-SIGN hD1 to that of three CPPs. The three CPPs colocalized within endosomes when targeted to human monocyte-derived DCs simultaneously, whereas hD1 was present in a different set of endosomes. However, within 75 min after uptake CPPs and hD1 colocalized extensively within the lysosomal compartment. Ab-mediated targeting of class I-restricted peptides to DC-SIGN enhanced cross-presentation of the peptides, while only one of the CPPs enhanced peptide presentation. This CPP and hD1 enhanced cross-presentation with equal efficiencies. Thus, we found no evidence of CPP specifically favoring the delivery of conjugated Ag to the DC class I presentation pathway. Given the specificity with which Abs recognize their targets, this favors the use of DC receptor-specific Abs for in vivo vaccination strategies.
The Language of Social Support in Social Media and Its Effect on Suicidal Ideation Risk (2017)

Online social support is known to play a significant role in mental well-being. However, current research is limited in its ability to quantify this link. Challenges exist due to the paucity of longitudinal, pre- and post mental illness risk data, and reliable methods that can examine causality between past availability of support and future risk. In this paper, we propose a method to measure how the language of comments in Reddit mental health communities influences risk to suicidal ideation in the future. Incorporating human assessments in a stratified propensity score analysis based framework, we identify comparable subpopulations of individuals and measure the effect of online social support language. We interpret these linguistic cues with an established theoretical model of social support, and find that esteem and network support play a more prominent role in reducing forthcoming risk. We discuss the implications of our work for designing tools that can improve support provisions in online communities.
Rotor Aerodynamic Design (2011)

The practical implications of optimum rotor theory for blade design are considered. Rotor solidity is related to design of rotors for variable speed operation and the interconnection of solidity with loads is briefly discussed. For optimum rotors, Reynolds number is shown to decrease with increasing design tip speed and be relatively constant over the blade span. Background is provided on the development of aerofoil designs to suit wind turbine applications. The sensitivity of aerodynamic performance to planform shape is illustrated as indicating the scope for trade-off between aerodynamic efficiency and rotor structural benefit. Finally an aerofoil design specification for operation with pitch control is suggested.
Crystal Structure of a Nucleotide Excision Repair Enzyme UvrB from <i>Thermus thermophilus</i> HB8 (2000)

Conference Abstract| October 01 2000 Crystal Structure of a Nucleotide Excision Repair Enzyme UvrB from Thermus thermophilus HB8 N. Nakagawa; N. Nakagawa 1Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka 560–0043, Japan Search for other works by this author on: This Site PubMed Google Scholar M. Sugahara; M. Sugahara 1Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka 560–0043, Japan Search for other works by this author on: This Site PubMed Google Scholar R. Masui; R. Masui 1Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka 560–0043, Japan Search for other works by this author on: This Site PubMed Google Scholar R. Kato; R. Kato 1Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka 560–0043, Japan Search for other works by this author on: This Site PubMed Google Scholar K. Fukuyama; K. Fukuyama 1Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka 560–0043, Japan Search for other works by this author on: This Site PubMed Google Scholar S. Kuramitsu S. Kuramitsu 1Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka 560–0043, Japan2Genomic Sciences Center and Harima Institute/SPring8, RIKEN, Japan Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (2000) 28 (5): A167. https://doi.org/10.1042/bst028a167a Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter LinkedIn Cite Icon Cite Get Permissions Citation N. Nakagawa, M. Sugahara, R. Masui, R. Kato, K. Fukuyama, S. Kuramitsu; Crystal Structure of a Nucleotide Excision Repair Enzyme UvrB from Thermus thermophilus HB8. Biochem Soc Trans 1 October 2000; 28 (5): A167. doi: https://doi.org/10.1042/bst028a167a Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu nav search search input Search input auto suggest search filter All ContentAll JournalsBiochemical Society Transactions Search Advanced Search This content is only available as a PDF. © 2000 Biochemical Society2000 Article PDF first page preview Close Modal You do not currently have access to this content.
A SECURE WEB APPLICATION PROVIDING PUBLIC ACCESS TO HIGH-PERFORMANCE DATA INTENSIVE SCIENTIFIC RESOURCES - ScalaBLAST Web Application (2008)

This work presents the ScalaBLAST Web Application (SWA), a web based application implemented using the PHP script language, MySQL DBMS, and Apache web server under a GNU/Linux platform. SWA is an application built as part of the Data Intensive Computer for Complex Biological Systems (DICCBS) project at the Pacific Northwest National Laboratory (PNNL). SWA delivers accelerated throughput of bioinformatics analysis via high-performance computing through a convenient, easy-to-use web interface. This approach greatly enhances emerging fields of study in biology such as ontology-based homology, and multiple whole genome comparisons which, in the absence of a tool like SWA, require a heroic effort to overcome the computational bottleneck associated with genome analysis. The current version of SWA includes a user account management system, a web based user interface, and a backend process that generates the files necessary for the Internet scientific community to submit a ScalaBLAST parallel processing job on a dedicated cluster.
Optical constants of In0.53Ga0.47As/InP: Experiment and modeling (2002)

The optical constants ε(E)=ε1(E)+iε2(E) of unintentionally doped In0.53Ga0.47As lattice matched to InP have been measured at 300 K using spectral ellipsometry in the range of 0.4 to 5.1 eV. The ε(E) spectra displayed distinct structures associated with critical points at E0 (direct gap), spin-orbit split E0+Δ0 component, spin-orbit split E1, E1+Δ1, E0′ feature, as well as E2. The experimental data over the entire measured spectral range (after oxide removal) has been fit using the Holden model dielectric function [Holden et al., Phys. Rev. B 56, 4037 (1997)], plus a Kramers–Kronig consistent correction, described in this work, that improves the fitting at low energies. This extended model is based on the electronic energy-band structure near these critical points plus excitonic and band-to-band Coulomb-enhancement effects at E0, E0+Δ0, and the E1, E1+Δ1, doublet. In addition to evaluating the energies of these various band-to-band critical points, information about the binding energy (R1) of the two-dimensional exciton related to the E1, E1+Δ1 critical points was obtained. The value of R1 was in good agreement with effective mass/k⋅p theory. The ability to evaluate R1 has important ramifications for first-principles band-structure calculations that include exciton effects at E0, E1, and E2 [M. Rohlfing and S. G. Louie, Phys. Rev. Lett. 81, 2312 (1998); S. Albrecht et al., Phys. Rev. Lett. 80, 4510 (1998)].
On-Demand Urine Analyzer (2010)

A lab-on-a-chip was developed that is capable of extracting biochemical indicators from urine samples and generating their surface-enhanced Raman spectra (SERS) so that the indicators can be quantified and identified. The development was motivated by the need to monitor and assess the effects of extended weightlessness, which include space motion sickness and loss of bone and muscle mass. The results may lead to developments of effective exercise programs and drug regimes that would maintain astronaut health. The analyzer containing the lab-on-a- chip includes materials to extract 3- methylhistidine (a muscle-loss indicator) and Risedronate (a bone-loss indicator) from the urine sample and detect them at the required concentrations using a Raman analyzer. The lab-on- a-chip has both an extractive material and a SERS-active material. The analyzer could be used to monitor the onset of diseases, such as osteoporosis.
A Continuously Adjustable Filter for Audiofrequencies (1949)

An electronic instrument has been developed to operate as a filter with cut-off frequencies continuously adjustable over the audio spectrum. The circuit consists of a low-pass and a high-pass network in the form of four-terminal impedances. These may be used singly or connected in tandem to give band pass action. A single control selects the cut-off frequency of each network over a range of 10 to 1, while a decade switch extends the over-all range to 100 to 1, or more. The low pass section provides a pass band flat within 1 db below the cut-off frequency. Attenuation past cut-off is at the rate of 18 db per octave down to the noise level of the circuit. The high-pass section provides a pass band flat within 2 db above the cut-off frequency. Suppression is more than 32 db for the first octave below cut-off, and the attenuation continues to increase down to the noise level. Noise is at least 60 db below a signal of one volt. The voltage attenuation in the pass band of the complete filter is about 6 db. The principle of operation of the instrument is based on active feedback networks using only resistance and capacitance. The variable elements are ganged resistors, whose values may be in error by several percent without affecting the performance described. Because there are no high gain stages in the circuit, distortion and noise problems are reduced to a minimum.
Design and Analysis of a Permanent Magnet Spherical Actuator (2008)

This paper has proposed a 3-DOF spherical actuator consisting of a ball-shaped rotor with a full circle of permanent- magnet (PM) poles and a spherical-shell-like stator with two layers of circumferential air-core coils. One key feature of this design is the parametrization of PM and coil poles. Based on the torque model of the PM spherical actuator, the relationship between poles' parameters and torque output can be demonstrated. As a result, the actuator design aiming at achieving maximum torque output can be carried out from the relationships. Another advantage of this spherical actuator is its singularity-free workspace, which is verified with the actuator torque model and condition numbers.
Nursing’s Voice in Healthcare IT Acquisition Decisions (2014)

Allen McLean PhD (c), RN, MN, MSc is currently a PhD student at the University of Saskatchewan in the Computational Epidemiology and Public Health Informatics Lab. He is researching potential applications of computer modeling and simulation for addressing chronic diseases and health inequities at the population level. Allen previously attended the University of Victoria earning an MN and MSc (Health Information Science) in a unique program for Nursing Informatics professionals. Allen has over 20 years’ experience in healthcare as a Navy medic, ultrasound technologist, and community health RN.​
Long‐term results of arthroscopic rotator cuff repair (SS‐07) (2004)

We have been performing arthroscopic rotator cuff repair since 1991. Short-term results have been previously presented and have been encouraging. Presented here are long-term results (minimum of 8 years postoperative) in a consecutive series of 64 patients who underwent 64 arthroscopic rotator cuff repairs. Three patients had died and 6 could not be located, leaving 55 patients who were reevaluated. We were able to personally interview and examine 48 patients; 7 patients were interviewed by telephone. The average age of these patients was 68 years. The repair technique used in these patients was predominantly our older technique utilizing No. 1 PDS and push-in anchors. Most of the tears were nonretracted crescent shaped tears and were repaired directly end to bone. We have traditionally evaluated our patients using the Modified UCLA (MUCLA) scoring system (45 points maximum). This group of patients was also evaluated using this scoring system. Eight year postoperative American Shoulder and Elbow Society and Constant scores were also determined for this group. For MUCLA scoring, we were able to compare the preoperative scores with the results of our previously published evaluation at 2–3 years and our current evaluation at 8 years. The average preoperative MUCLA score was 17, the 2–3 year score was 41, and the 8 year score was also 41. Excellent results were achieved in 68% at 2–3 years and in 75% at 8 years. Good results were obtained in 25% at 2–3 years and 10% at 8 years. Fair results were obtained in 2% of both 2–3 year and 8 year follow-up groups. Poor results were recorded in 5% at 2–3 years and 13% at 8 years. American Shoulder and Elbow Society Scores at 8 years were as follows: 90.8 average overall score (range 40–100), Average pain score was 0.9. Average ADL score was 27.3. Average strength score was 4.8 (abduction) and 4.9 (IR and ER). Constant Scores at 8 years were as follows: 91.3 average overall score (range 68–100). Average pain score was 13. Average total ADL score was 18.8. Average ROM score was 36.1. Average strength score was 12.3. Average strength score when compared to the unaffected side (operated side score × 25/unaffected side score) was 22.4. We used this comparison value to determine the overall average score. Overall, the results of arthroscopic rotator cuff repair are very good and have shown to be durable at 8 year follow-up. These results compare well to the long-term results of open repairs. It must be noted, however, that these are the results of the repair of only the smaller tears that were undertaken at the time. Currently we are using stronger sutures with higher pull-out strength anchors and are repairing much larger tears. The short-term results of these repairs are also good but their long-term durability must still be determined.
New interpretations of Wiener filters for image recognition (1996)

Wiener filters for restoration of images and Wiener filters for image recognition are well established. We present the relations between two versions of Wiener restoration filters and develop new conceptual interpretations of the Wiener recognition filter, in the process of which interesting features are revealed. In one interpretation we show that the correlation signal resulting from Wiener recognition filtering may be regarded as the restoration of a hidden delta function. In another interpretation we regard the Wiener recognition filter as a two-filter scheme whereby an inverse filter and a Wiener restoration filter are operated sequentially. Results of simulations of the latter representation are presented for various input-noise models, providing useful insight into the implications of such an interpretation.
[Speckle tracking analysis of left atrial phasic function in patients with hypertension]. (2014)

LA reservoir and conduit functions are impaired in HT and deteriorate with left ventricular diastolic dysfunction. LA contraction is preserved in HT with normal and mild diastolic dysfunction while impaired in moderate/severe diastolic dysfunction.
Performance evaluation of UGV obstacle detection with CCD/FLIR stereo vision and LADAR (2002)

The next phase of unmanned ground vehicle (UGV) development, the Demo III programme, aims to enable round-the-clock operation with autonomous, cross-country navigation at speeds up to 40 mph on roads, 20 mph by day off road, and 10 mph by night off road. This paper reviews the obstacle sizes that must be detected and lookahead distances that are required to support such driving speeds, and describes data collection and performance evaluation efforts with three different range imaging systems: 1) stereo vision with CCD cameras, 2) stereo vision with InSb FLIR cameras operating in the 3-5 /spl mu/m band, and 3) the Dornier EBK LADAR. The LADAR and the FLIR stereo are applicable to day and night operation; the CCD stereo to day operation only. This paper describes the data set and preliminary obstacle detection results obtained. Ongoing performance evaluation with this data set will guide obstacle detection sensor selection and development for the Demo III Programme.
Heat shock protein 90 is required for <i>ex vivo</i> neutrophil‐driven autoantibody‐induced tissue damage in experimental epidermolysis bullosa acquisita (2015)

A broad range of immunosuppressive and immunomodulatory effects of heat shock protein 90 (Hsp90) blockade has been described in models of autoimmune bullous diseases, but the direct contribution of this chaperone to neutrophil effector pathways in the context of autoantibody-driven blistering is generally unknown. Therefore, this has been addressed in the current study on the basis of the subepidermal blistering disease epidermolysis bullosa acquisita (EBA) characterized by autoantibodies against type VII collagen, in which a crucial role of neutrophils and both their reactive oxygen species and matrix metalloproteinases in mediating tissue injury has been established. First, the Hsp90 antagonist 17-DMAG dose-dependently inhibited dermal-epidermal separation ex vivo in cryosections of human skin induced by co-incubation of EBA patient autoantibodies with neutrophils from healthy blood donors. Next, 17-DMAG dose-dependently suppressed production and release of reactive oxygen species by human neutrophils induced by both fMLP ± LPS and EBA-specific immune complexes. In addition, co-immunoprecipitation studies revealed that extracellular Hsp90 interacted with secreted matrix metalloproteinases 2 and 12 in sera of EBA patients, suggesting that these basement membrane-degrading proteolytic enzymes are client proteins of Hsp90 and dependent on its chaperone function. Our findings add to the knowledge of the multimodal anti-inflammatory effects of Hsp90 blockade and implicate that Hsp90 is closely involved in the effector mechanisms of neutrophil-driven autoantibody-induced tissue damage, thus being a relevant therapeutic target in patients with neutrophil-mediated autoimmune diseases such as inflammatory types of EBA.
Growth Hormone Response During Sandfly Fever (1968)

Journal Article Growth Hormone Response During Sandfly Fever Get access WILLIAM R. BEISEL, WILLIAM R. BEISEL 1U. S. Army Medical Unit, Fort Detrick Frederick, Maryland 21701; Thorndike Memorial Laboratory, Second and Fourth (Harvard) Medical Services, Boston City Hospital Boston, Massachusetts; Department of Medicine, Harvard Medical School Boston, Massachusetts 02118 Search for other works by this author on: Oxford Academic Google Scholar KENNETH A. WOEBER, KENNETH A. WOEBER 1U. S. Army Medical Unit, Fort Detrick Frederick, Maryland 21701; Thorndike Memorial Laboratory, Second and Fourth (Harvard) Medical Services, Boston City Hospital Boston, Massachusetts; Department of Medicine, Harvard Medical School Boston, Massachusetts 02118 Search for other works by this author on: Oxford Academic Google Scholar PETER J. BARTELLONI, PETER J. BARTELLONI 1U. S. Army Medical Unit, Fort Detrick Frederick, Maryland 21701; Thorndike Memorial Laboratory, Second and Fourth (Harvard) Medical Services, Boston City Hospital Boston, Massachusetts; Department of Medicine, Harvard Medical School Boston, Massachusetts 02118 Search for other works by this author on: Oxford Academic Google Scholar SIDNEY H. INGBAR SIDNEY H. INGBAR 1U. S. Army Medical Unit, Fort Detrick Frederick, Maryland 21701; Thorndike Memorial Laboratory, Second and Fourth (Harvard) Medical Services, Boston City Hospital Boston, Massachusetts; Department of Medicine, Harvard Medical School Boston, Massachusetts 02118 Search for other works by this author on: Oxford Academic Google Scholar The Journal of Clinical Endocrinology & Metabolism, Volume 28, Issue 8, 1 August 1968, Pages 1220–1223, https://doi.org/10.1210/jcem-28-8-1220 Published: 01 August 1968 Article history Received: 20 February 1968 Accepted: 29 April 1968 Published: 01 August 1968
Growth and characterization of strontium-substituted La<sub>3</sub>Ta<sub>0.5</sub>Ga<sub>5.5</sub>O<sub>14</sub> single crystals (2021)

The effects of strontium substitution on the electrical properties of La3Ta0.5Ga5.5O14 (LTG) are reported. Strontium substituted LTG (Sr-LTG) with a nominal chemical composition La2.9Sr0.1Ta0.55Ga5.45O14 crystals were grown using the conventional Czochralski method under high and low oxygen partial pressures. The electrical properties and thermal behaviors of the Sr-LTG crystals were also compared with those of pure crystals. Sr substitution was found to affect the electroacoustic constants and thermal expansion coefficient clearly. These characteristics are discussed from crystallographic perspectives. The Sr-LTG crystals grown at low oxygen partial pressures exhibited higher electrical resistivity ρ for pressure sensors used at high temperatures.
OVEREXPRESSION OF C-MYC, C-FOS AND HARVEY RAS ONCOGENES IN RENAL CANCERS (1991)

The accumulating data have shown that a single or certain combinations of proto-oncogenes are genetically altered and acquire oncogenic activities in certain tumor types, through a point mutation and/or overexpression. In the present study, Northern blotting analysis was applied to clinical samples of renal cell carcinomas (RCC) to elucidate expression levels of the c-myc, c-fos and Harvey ras genes in this tumor form. In 11 cases of 23 tumors examined (48%) was shown more than 3-fold expression level of the c-myc gene compared with the matching normal RNA, the c-fos gene in 6 cases (26%), and the Harvey ras gene in one case (4%) showing the concomitant overexpression of two other genes. Overexpression of both the c-myc and c-fos genes was detected in 5 cases (22%). Although the distinct correlation between overexpressions of any genes and the clinical background was not induced, it is considered that these aberrations of proto-oncogenes are involved in oncogenesis of a certain subgroup of RCC.
Accurate characterization of electron and hole inversion-layer capacitance and its impact on low voltage operation of scaled MOSFETs (2002)

The influence of inversion-layer capacitance (C/sub inv/) on supply voltage (V/sub dd/) of n- and p-MOSFETs is quantitatively examined. Hole C/sub inv/ is experimentally evaluated in addition to electron C/sub inv/. It is found that the degradation of gate capacitance for holes due to C/sub inv/, is more severe than that for electrons. Self-consistent calculation under a simple valence band model represents the experimental hole C/sub inv/, quite well. It is demonstrated that additional band bending of a Si substrate due to C/sub inv/ becomes a dominant factor to limit the lowering of V/sub dd/ for CMOS with ultra-thin gate oxides. The operation at V/sub dd/ lower than 0.6 V is quite difficult even with effective T/sub OX/ less than 1 nm.