gebhart/patrepeval huggingface dataset:
Silencing of antiapoptotic survivin gene by multiple approaches of RNA interference technology (2004)

Silencing of mammalian gene expression by RNA interference (RNAi) technology can be achieved using small interfering RNA (siRNA) or short hairpin RNA (shRNA). However, the relative effectiveness of these two approaches is not known. It is also not clear whether gene-specific shRNA transcribed from an RNA polymerase II (Pol II)-directed promoter in a fusion form can disrupt the targeted gene expression. Here, we report that using both luciferase and antiapoptotic survivin genes as targets, both siRNA and shRNA approaches significantly silenced the targeted gene expression in cancer cells. We further demonstrated that shRNAs transcribed from an RNA Pol II-mediated promoter in a green fluorescent protein (GFP) fusion form at the 3'-untranslated region silenced luciferase and survivin expression as well, suggesting that the extra RNA sequence outside of the shRNA hairpin does not disrupt shRNA function. We also showed that silencing of survivin expression selectively induces apoptosis in transfected cells. Together, we have validated multiple approaches of RNAi technology using both survivin and luciferase genes as targets and demonstrated for the first time that GFP-shRNAs transcribed from an RNA Pol II-mediated promoter could mediate gene silencing, which may lead to new directions for the application of RNAi technology.
Contributors (Apr. 1957 [T-MTT]) (1957)

Contains an entry for each author and coauthor included in this issue of the publication.
The Stream of Violence: A New Approach to Domestic Violence Personal Jurisdiction (2016)

There is a split among state courts about whether personal jurisdiction over an alleged domestic violence perpetrator is required in order to obtain a civil protection order preventing the defendant from contacting the victim. Some courts have held that such orders interfere with the defendant’s liberty interests, and therefore the Due Process Clause requires personal jurisdiction for the issuance of such orders. Other courts have held that personal jurisdiction is not required because such protection orders are analogous to custody and divorce orders, which, under the “status exception,” courts have historically entered without establishing personal jurisdiction over the other party. This Article argues that the focus on the status exception is misplaced and that instead, courts should reframe the way they look at personal jurisdiction in domestic violence cases by applying the principles embedded in the stream of commerce doctrine and the effects test. Drawing upon common threads from each line of cases, the Article proposes a test for domestic violence jurisdiction that focuses on the knowledge of the defendant about the victim’s likely destination if she is forced to flee to another state.
Evidence That Pretranslational and Translational Defects Decrease Serum Insulin-Like Growth Factor-I Concentrations during Dietary Protein Restriction* (1991)

Dietary protein restriction causes GH resistance and decreases serum insulin-like growth factor-I (IGF-I) concentrations. To determine whether pretranslational or translational defects are involved in the decline of serum IGF-I concentrations during protein restriction, we measured hepatic IGF-I mRNA abundance together with the serum IGF-I peptide response to exogenous GH after 1 week of protein restriction (5% casein in diet; P5) in hypophysectomized rats. We compared these responses with those of hypophysectomized rats fed a protein-sufficient diet (15% casein in diet; P15) and given exogenous GH. A single injection of rat GH (200 micrograms/100 g BW) produced a comparable IGF-I mRNA increment in both groups (at 6 h, 7.8 +/- 1.1 arbitrary units in P5 vs. 8.2 +/- 1.1 in P15), but failed to raise serum IGF-I normally in the P5 group (at 6 h, 90 +/- 15 ng/ml in P5 vs. 216 +/- 63 in P15; P less than 0.01). The post-GH decline of the 7.5-kilobase (kb) IGF-I mRNA abundance was faster in P5 than in P15 animals. In another experiment in intact rats subjected to protein restriction, injections of pharmacological doses of rat GH (400 micrograms/100 g BW.day) for 1 week restored liver IGF-I mRNA abundance to normal without normalization of serum IGF-I (403 +/- 91 vs. 713 +/- 53 ng/ml; P less than 0.01). Our data suggest that 1) the machinery involved in the transcription of the liver IGF-I gene is intact in protein-restricted rats, because these animals retain the ability to muster normal IGF-I mRNA responses to high doses of exogenous GH; 2) the stability of the 7.5-kb IGF-I mRNA is probably decreased by the protein restriction, as suggested by the faster decline of the 7.5-kb transcript in P5 than in P15 hypophysectomized rats; and 3) the discrepancy between normal liver IGF-I mRNA abundance and low serum and liver IGF-I peptide concentrations suggests that translational stalling of the IGF-I mRNAs or increased serum IGF-I clearance is involved in the low serum IGF-I concentrations during dietary protein restriction.
Potential of Anti- <i>Candida</i> Antibodies in Immunoprophylaxis (2010)

The need for new options for the treatment of invasive candidiasis has fuelled the use of antibodies in combination with conventional antifungal therapy. After a long period of time in which antibodies were considered irrelevant in the resistance against invasive candidiasis, it was demonstrated that a number of antibodies or their engineered derivatives directed against Candida albicans cell-wall polysaccharides and glycopeptides, as well as against some protein epitopes, confer protection against invasive candidiasis. This has confirmed this approach as a new strategy for the prophylaxis of invasive candidiasis. Of particular interest is Mycograb, a human recombinant monoclonal antibody that inhibits heat shock protein 90, and has been administrated in combination with lipid-associated amphotericin B to patients with invasive candidiasis, and the fungicidal anti-beta-glucan antibodies induced by the glycoconjugate vaccine composed of a beta-glucan polysaccharide conjugated with the diphtheria toxoid CRM 197. However, despite the promising data obtained in vitro and in animal models, at present there is very little clinical experience on the use of antibodies in Candida immunoprophylaxis.
Environmental Effect Caused by Over-exploitation of Deep Groundwater in North China (2014)

Based on the formation and development analysis of the environmental geological disaster of land subsidence, earth fissures and other geological disasters in the North China，it showed these disasters caused a very serious problem in some areas of the North China, such as the deep groundwater exploitation cone, which is accumulated with great damage and loss and is hard to be controlled, therefore, great attention should be paid. It is considered that the formation of the deep groundwater cone is the root of various geological environmental problems, and the groundwater cone recovery is the key to solve other environmental problems.
Solution structure of a paradigm ArsR family zinc sensor in the DNA-bound state (2009)

Staphylococcus aureus CzrA is a zinc-dependent transcriptional repressor from the ubiquitous ArsR family of metal sensor proteins. Zn(II) binds to a pair of intersubunit C-terminal alpha5-sensing sites, some 15 A distant from the DNA-binding interface, and allosterically inhibits DNA binding. This regulation is characterized by a large allosteric coupling free energy (DeltaGc) of approximately +6 kcal mol(-1), the molecular origin of which is poorly understood. Here, we report the solution quaternary structure of homodimeric CzrA bound to a palindromic 28-bp czr operator, a structure that provides an opportunity to compare the two allosteric "end" states of an ArsR family sensor. Zn(II) binding drives a quaternary structural switch from a "closed" DNA-binding state to a low affinity "open" conformation as a result of a dramatic change in the relative orientations of the winged helical DNA binding domains within the dimer. Zn(II) binding also effectively quenches both rapid and intermediate timescale internal motions of apo-CzrA while stabilizing the native state ensemble. In contrast, DNA binding significantly enhances protein motions in the allosteric sites and reduces the stability of the alpha5 helices as measured by H-D solvent exchange. This study reveals how changes in the global structure and dynamics drive a long-range allosteric response in a large subfamily of bacterial metal sensor proteins, and provides insights on how other structural classes of ArsR sensor proteins may be regulated by metal binding.
High capacity cellular patterns for land mobile radio systems using directional antennas (1989)

To meet increasing demand for mobile communications with a fixed spectrum and at reasonable cost, directional antennas have been used in cellular mobile radio systems. This paper reviews sectorised patterns that have been proposed so far, and then describes how to achieve high capacity systems by taking a new approach to the design of cellular patterns. Some novel patterns, with less than or equal to two hexagons per cluster are described. Performances are assessed in terms of the first decile carrier-to-cochannel interference (C/Ic) and traffic density for a given blocking probability. Cell site efficiency is also considered.
A Comprehensive Analysis of MAC Enhancements for Leveraging Distributed MAC (2008)

Abstract—Increased dependability of users, businesses and government on computing systems for research and computations on sensitive data raises serious issues regarding security. Operating systems conventionally provide Discretionary Access Control (DAC, superuser logic) to maintain security. Malicious users and applications are major threats to organizations and DAC seriously fails to address the required level of security. Former research has proven that enforcement of Mandatory Access Control (MAC, security labels/contexts for subjects and objects) restricts the malicious agents to their own domain, thus eliminating risk of damage it can cause to rest of the system and data. MAC implementations depend upon access control mechanisms. These mechanisms are added to operating systems as kernel enhancements. There are four prominent kernel enhancements: RSBAC, SELinux, GRSecurity and AppArmor. This paper assesses and reviews the differences in their implementation and enforcement techniques to achieve their similar goals and then compares their effectiveness. We give special attention to the implementation details and network labeling, in order to evaluate and understand this underlying layer of security. Our research objective is to propose a framework for Distributed MAC (DMAC), which depends on a clear perception of the architecture, internals and features of these enhancements.
Sucking Technique and Its Effect on Success of Breastfeeding (1992)

We investigated the prognostic value of sucking technique (faulty vs correct) during the first week after birth in relation to the long-term success of breastfeeding. At discharge from the maternity ward, 82 healthy mother-infant pairs were observed for assessment of breastfeeding technique and followed for four months by regular telephone checkups. Correct sucking technique was defined as the infant having a wide-open mouth, with the tongue under the areola, and expressing milk from the breast by slow, deep sucks; faulty technique was defined as superficial nipple sucking. The study population was divided into three groups: one in which faulty sucking technique was corrected when observed (n = 29), one with faulty but uncorrected technique (n = 25), and a control group with a correct technique (n = 28). At the four-month follow-up assessment, the faulty but uncorrected group was characterized by a greater proportion of mothers with breastfeeding problems and early cessation of breastfeeding than the other two groups. Regular use of a pacifier (> 2 hrs/day) was more common among those with breastfeeding problems.
Evil maid goes after PGP whole disk encryption (2010)

Full disk encryption systems are widely used to protect the information from unauthorized access. A common application of such systems is laptop hard drive and removable media encryption, because these can be easily lost or stolen. Indeed, if we assume that an encryption system used by the FDE software is cryptographically strong, correctly implemented and properly used, and that the attacker does not possess a key for the stolen media then the data is safe. However, given physical access to the laptop (which is powered off to ensure that no keys remain in memory), an attacker can do other things besides stealing it: for example, he can modify its disk contents (e.g. an FDE loader code), leaving the laptop to the unsuspecting owner. Next time, when the password or a key will be provided by the owner, the code left by the attacker may silently record the decryption key and send it to the attacker. This type of physical attacks is called "Evil Maid" because such attacks can be easily conducted by a hotel maid when the owner leaves a laptop unattended in the room for a short period of time. This is why it is essential for a FDE system to assure the user that the system that just booted is actually the system that he or she wanted to boot (i.e. the trusted one) and not some modified system (e.g. compromised by an MBR virus). This is called trusted boot. Trusted boot can be implemented using either a Static Root of Trust or a Dynamic Root of Trust.
Lossless data compression of grid-based digital elevation models: A png image format evaluation (2014)

Abstract. At present, computers, lasers, radars, planes and satellite technologies make possible very fast and accurate topographic data acquisition for the production of maps. However, the problem of managing and manipulating this data efficiently remains. One particular type of map is the elevation map. When stored on a computer, it is often referred to as a Digital Elevation Model (DEM). A DEM is usually a square matrix of elevations. It is like an image, except that it contains a single channel of information (that is, elevation) and can be compressed in a lossy or lossless manner by way of existing image compression protocols. Compression has the effect of reducing memory requirements and speed of transmission over digital links, while maintaining the integrity of data as required. In this context, this paper investigates the effects of the PNG (Portable Network Graphics) lossless image compression protocol on floating-point elevation values for 16-bit DEMs of dissimilar terrain characteristics. The PNG is a robust, universally supported, extensible, lossless, general-purpose and patent-free image format. Tests demonstrate that the compression ratios and run decompression times achieved with the PNG lossless compression protocol can be comparable to, or better than, proprietary lossless JPEG variants, other image formats and available lossless compression algorithms.
Acrylamide in Foods: A Review of the Science and Future Considerations (2012)

Acrylamide occurs in foods commonly consumed in diets worldwide. It is formed from the reaction of reducing sugars (e.g., glucose or fructose) with the amino acid asparagine via the Maillard reaction, which occurs during heat processing of foods, primarily those derived from plant origin, such as potato and cereal products, above 120°C (248°F). The majority of epidemiological studies concerning potential relationships between acrylamide consumption and different types of cancer have indicated no increased risk, except with a few types that warrant further study. Efforts to reduce the formation of acrylamide in food products have resulted in some successes, but there is no common approach that works for all foods. Reduction in some foods is probably not possible. The results from a major toxicological study (aqueous intake of acrylamide by rats and mice) are in the process of being released. The status of current knowledge in these areas is reviewed.
Blocking the <scp>GABA</scp> transporter <scp>GAT</scp>‐1 ameliorates spinal <scp>GABA</scp>ergic disinhibition and neuropathic pain induced by paclitaxel (2015)

Abstract Paclitaxel is a chemotherapeutic agent widely used for treating carcinomas. Patients receiving paclitaxel often develop neuropathic pain and have a reduced quality of life which hinders the use of this life‐saving drug. In this study, we determined the role of GABA transporters in the genesis of paclitaxel‐induced neuropathic pain using behavioral tests, electrophysiology, and biochemical techniques. We found that tonic GABA receptor activities in the spinal dorsal horn were reduced in rats with neuropathic pain induced by paclitaxel. In normal controls, tonic GABA receptor activities were mainly controlled by the GABA transporter GAT ‐1 but not GAT ‐3. In the spinal dorsal horn, GAT ‐1 was expressed at presynaptic terminals and astrocytes while GAT ‐3 was only expressed in astrocytes. In rats with paclitaxel‐induced neuropathic pain, the protein expression of GAT ‐1 was increased while GAT ‐3 was decreased. This was concurrently associated with an increase in global GABA uptake. The paclitaxel‐induced attenuation of GABA ergic tonic inhibition was ameliorated by blocking GAT ‐1 but not GAT ‐3 transporters. Paclitaxel‐induced neuropathic pain was significantly attenuated by the intrathecal injection of a GAT ‐1 inhibitor. These findings suggest that targeting GAT ‐1 transporters for reversing disinhibition in the spinal dorsal horn may be a useful approach for treating paclitaxel‐induced neuropathic pain. image Patients receiving paclitaxel for cancer therapy often develop neuropathic pain and have a reduced quality of life. In this study, we demonstrated that animals treated with paclitaxel develop neuropathic pain, have enhancements of GABA transporter‐1 protein expression and global GABA uptake, as well as suppression of GABAergic tonic inhibition in the spinal dorsal horn. Pharmacological inhibition of GABA transporter‐1 ameliorates the paclitaxel‐induced suppression of GABAergic tonic inhibition and neuropathic pain. Thus, targeting GAT‐1 transporters for reversing GABAergic disinhibition in the spinal dorsal horn could be a useful approach for treating paclitaxel‐induced neuropathic pain.
Value of p53 immunostaining in pancreatico-biliary brush cytology specimens (2000)

Brush cytology is routinely used in the assessment of pancreatico-biliary strictures but the technique has limited diagnostic sensitivity in malignant lesions. It has been suggested that ancillary techniques, such as the identification of p53 immunoreactivity, might improve diagnostic accuracy. p53 protein expression was examined in 143 consecutive brush cytology specimens from patients with pancreatic or bile duct strictures and correlated with conventional cytological assessment and clinicopathologic follow-up data. Sixty-three of 89 (70.8%) malignant strictures were identified cytologically while 45 cases (50.6%) were p53 immunoreactive. One case of bile duct adenoma with high-grade dysplasia was reported as consistent with adenocarcinoma cytologically and was p53 negative. There was one false-positive diagnosis with conventional cytology and, in a separate case, with p53 immunostaining. Nineteen specimens (13.3%) were considered atypical cytologically and p53 expression proved accurate in only 12 cases (four immunopositive carcinomas and eight negative benign strictures). In conclusion, p53 immunostaining proved less sensitive than conventional cytology in this series and its routine diagnostic use could not be supported.
Selenium in the Environment, Metabolism and Involvement in Body Functions (2013)

Selenium (Se³⁴₇₉) is a metalloid which is close to sulfur (S) in terms of properties. The Se concentration in soil varies with type, texture and organic matter content of the soil and with rainfall. Its assimilation by plants is influenced by the physico-chemical properties of the soil (redox status, pH and microbial activity). The presence of Se in the atmosphere is linked to natural and anthropogenic activities. Selenoproteins, in which selenium is present as selenocysteine, present an important role in many body functions, such as antioxidant defense and the formation of thyroid hormones. Some selenoprotein metabolites play a role in cancer prevention. In the immune system, selenium stimulates antibody formation and activity of helper T cells, cytotoxic T cells and Natural Killer (NK) cells. The mechanisms of intestinal absorption of selenium differ depending on the chemical form of the element. Selenium is mainly absorbed in the duodenum and caecum by active transport through a sodium pump. The recommended daily intake of selenium varies from 60 μg/day for women, to 70 μg/day for men. In growing ruminants the requirements are estimated at 100 μg/kg dry matter and 200 μg/Kg for pregnant or lactating females. A deficiency can cause reproductive disorders in humans and animals.
Tradeoff between energy and error in the discrimination of quantum-optical devices (2011)

We address the problem of energy-error tradeoff in the discrimination between two linear passive quantum optical devices with a single use. We provide an analytical derivation of the optimal strategy for beamsplitters and an iterative algorithm converging to the optimum in the general case. We then compare the optimal strategy with a simpler strategy using coherent input states and homodyne detection. It turns out that the former requires much less energy in order to achieve the same performances.
A method of extraction and separation of wheat gluten (2008)

After removing gliadin and other proteins with 7.5% 2-propanol and 0.3 mol/L NaI, the total glutenin subunits were extracted with two kinds of buffer, one containing 25% 2-propanol, 0.04 mol/L Tris-HCI (pH=8.0), 10% SDS and 2% DDT and the other containing 25% 2-propanol, 0.04 mol/L Tris-HCI (pH=8.0), 10% SDS and 1.4% VP. In SDS-PAGE electrophoresis (4% stacking gel and 13% resolving ge1) system HMW-GS and LMW-GS were clearly separated by the improved method with only a single step. The background of the SDS-PAGE gel was gliadin-free. It was more effective and applicable to separate and distinguish the complex LMW-GS. This method will be used in wheat protein separation, variety identification and improvement.
Doing it down under - the joys of sex (research) (2003)

The author presents the summary findings of the Australian Study of Health and Relationships, a large population-based survey of sexual health behaviours, attitudes and knowledge of Australian people. (non- author abstract)
Aldehyde-dehydrogenase gene-transduced hematopoietic cell line K562 overcomes the cytoxicity of cyclophosphamide in vitro. (2002)

The identification of genes inducing resistance to anticancer chemotherapeutic agents and their introduction into hematopoietic cells represents a promising approach to overcome bone marrow toxicity, the limiting factor for most high-dose chemotherapy regimens. Because resistance to cyclophosphamide has been correlated with increased levels of expression of the aldehyde-dehydrogenase (ALDH1) gene in tumor cells lines in vitro, this study tested whether ALDH1 overexpression could directly induce cyclophosphamide resistance. Results showed that a retroviral vector was used to transduce full-length human ALDH1 cDNA into human hematopoietic cell line K562 that was then tested for resistance to 4-hydroxycyclophosphamide (4-HC), an active analogue of cyclophosphamide. Overexpression of the ALDH1 gene resulted in a significant increases in cyclophosphamide resistance in transduced K562 cells (50% inhibition concentration, IC50 = 10 micro mol/L). These findings indicate that ALDH1 overexpression is sufficient to induce cyclophosphamide resistance in vitro and provide a basis for testing the efficacy of ALDH1 gene transduction to protect bone marrow cells from high-dose cyclophosphamide in vivo.