gebhart/patrepeval huggingface dataset:
Improved culture conditions forclonogenic growth of primary humanbreast tumours (1984)

SummaryFourestablished humanbreast tumourcell lines withdifferent biologic properties wereselected forstudy andrequirements fortheir clonogenic growth insemisolid cultures wereidentified. Theconventional conditions were modified byfactors thatenhanced colony formation of3 or more ofthesecelllines. The modified culture conditions were thenapplied tothegrowthinagarofprimary breast tumours. A 5-fold improvement inplating efficiency was observed whencultures of105primary tumoursgrownunderthese modified conditions were compared tothoseof52tumoursgrownearlier underconventional conditions, and a 4-fold improvement resulted fromtheaddition ofhormonesandconditioned medidumin26tumours cultured simultaneously underbothconditions. Thebiologic relevance oftheseclonogens recovered invitro was substantiated bya 70%concordance ofinvitro andinvivotumoursensitivity toanticancer drugs. Theclonogenic growth ofprimary humantumours isafield ofinterest forthestudy oftumourbiology andofinherent tumoursensitivity toanticancer drugs. Breast tumourcultures areofparticular interest because ofthecommonoccurrence ofthis tumourand because ofitsfrequent hormone dependence. However,notallprimarybreast tumoursformcolonies insemisolid cultures, and thenumberofcolonies ofthose thatdoisoften small(VonHoff etal., 1981; Sikic & Taber, 1981; Sandbach etal., 1982). Invitro growthofbreast tumoursisalsolimited inotherculture systems (Courtney & Mills, 1978). Smallproportions of clonogenic tumourcells ofprimary neoplasms or deficient culture conditions arepossible causes for failure. We investigated theeffect oncolony formation of nutrients bymodifying theculture conditions for breast tumours. Since established tumourcells have incommonwithtumourstemcells their tissue of origin, wereasoned that agroupofcell lines witha spectrum ofbiologic properties similar tothose of primary tumours mayserve asaprimary screen to identify thenutritional requirements ofprimary tumours. We haveinvestigated thishypothesis by identifying, ina systematic stepwise fashion, the culture conditions forclonogenic growthof4 selected breast tumourcelllines thatdiffered in growthkinetics, hormonaldependency and tumourgenicity: MCF-7,MDA-231,MDA-435and MDA-468 (TableI).MCF-7 has functional receptors for17-fl-estradiol, hydrocortisone, insulin andepidermal growth factor (Osborne & Lippman,
CHARACTERIZATION OF EEG SIGNAL CHANGES (1995)

Clinical assessment of neurological system would be greatly facilitated by interpretation of' disease- or injuryrelated changes in electroencephalogram (EEG) signals. Under the assumption that EEG signal can be modeled as an autoregressive (AR) process, Itha distance is used to measure the similarity of the EEG signals obtainedduring various phases of the experimental study. The effectiveness of the Itakura distance is demonstrated through its ability to distinguish hypoxia and asphyxia as well as to predict recovery following the iqiw.
Photon backscattering tissue characterization by energy dispersive spectroscopy evaluations (1994)

Techniques for in vivo tissue characterization based on scattered photons have usually been confined to evaluating coherent and Compton peaks. However, information can also be obtained from the energy analysis of the Compton scattered distribution. This paper looks at the extension of a technique validated by the authors for characterizing tissues composed of low-atomic-number elements. To this end, an EDXRS (energy dispersive x-ray spectrometry) computer simulation procedure was performed and applied to test the validity of a figure of merit able to characterize binary compounds. This figure of merit is based on the photon fluence values in a restricted energy interval of the measured distribution of incoherently scattered photons. After careful experimental tests with 59.54 keV incident photons at scattering angles down to 60degrees, the simulation procedure was applied to quasi-monochromatic and polychromatic high-radiance sources. The results show that the characterization by the figure of merit, which operates satisfactorily with monochromatic sources, is unsatisfactory in the latter cases, which seem to favour a different parameter for compound characterization.
“Water-stable boron-iodinated dicarbollide dianions [7,8-nido-C<sub>2</sub>H<sub>2</sub>B<sub>9</sub>I<sub>9</sub>]<sup>2−</sup>and [7,8-nido-C<sub>2</sub>H<sub>2</sub>B<sub>9</sub>I<sub>8</sub>H]<sup>2−</sup>” (2007)

The reaction of 3,4,5,7,8,9,10,11,12-I(9)-1,2-closo-C(2)B(10)H(3) with KOH/EtOH gave a mixture of the boron periodinated [1,2,3,4,5,6,9,10,11-I(9)-7,8-nido-C(2)B(9)H(2)](2-) and the highly iodinated on boron [1,2,4,5,6,9,10,11-I(8)-7,8-nido-C(2)B(9)H(3)](2-) in approximately 50% each. Moreover, 3,4,5,6,7,8,9,10,11,12-I(10)-1,2-closo-C(2)B(10)H(2) was reacted with KOH/EtOH to purely produce [1,2,3,4,5,6,9,10,11-I(9)-7,8-nido-C(2)B(9)H(2)](2-). It is the first dinegative dicarbollide stable in water or protic solvent reported in literature.
A partial-response receiver for Miller-squared encoded signals with half the usual operating speed (1990)

A new receiver for Miller-squared encoded signals that has a 50% lower operating speed than its predecessors, which reconstruct the encoded signal before decoding the original data, is described. The reduction is achieved by using partial-response techniques to detect a linearly transformed and decimated version of the encoded signal, from which the original data can be recovered by means of a simple decoder. Simulation results for stylized recording systems with differentiating and magnetoresistive playback heads are presented. Simulations confirm the feasibility of the proposed approach and indicate that the performance of the receiver improves upon that of its conventional counterpart under certain conditions.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Simulation of Compressible Flow on a Massively Parallel Architecture (1995)

This article describes the porting and optimization of an explicit, time‐dependent, computational fluid dynamics code on an 8,192‐node MasPar MP‐1. The MasPar is a very fine‐grained, single instruction, multiple data parallel computer. The code uses the flux‐corrected transport algorithm. We describe the techniques used to port and optimize the code, and the behavior of a test problem. The test problem used to benchmark the flux‐corrected transport code on the MasPar was a two‐dimensional exploding shock with periodic boundary conditions. We discuss the performance that our code achieved on the MasPar, and compare its performance on the MasPar with its performance on other architectures. The comparisons show that the performance of the code on the MasPar is slightly better than on a CRAY Y‐MP for a functionally equivalent, optimized two‐dimensional code.
1-Iodo-2-(perfluoroalkyl)cycloalkanes by the Free Radical Addition of Iodoperfluoroalkanes to Cyclohexane and Cyclopentene (1963)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXT1-Iodo-2-(perfluoroalkyl)cycloalkanes by the Free Radical Addition of Iodoperfluoroalkanes to Cyclohexane and CyclopenteneNeal O. BraceCite this: J. Org. Chem. 1963, 28, 11, 3093–3102Publication Date (Print):November 1, 1963Publication History Published online1 May 2002Published inissue 1 November 1963https://pubs.acs.org/doi/10.1021/jo01046a039https://doi.org/10.1021/jo01046a039research-articleACS PublicationsRequest reuse permissionsArticle Views224Altmetric-Citations44LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
On some properties of phytoalexin produced as a result of the interaction between pea (Pisum sativum L.) and Ascochyta pisi Lib (1960)

Experiments were carried out to study the effect of the length of time of incubation with spore suspension of Ascochyta pisi Libert, and of preinfectional treatments of pea pod with ether gas or hot water, on production of an antibiotic factor, phytoalexin, by pea pod. The methods in general are described in the previous paper. Germination test of Ascochyta spore for bioassay of phytoalexin, was modified to use agar blocks (5×5×1-0.5mm) suspended with conidia, in place of spore suspension.The antibiotic activity of diffusate could not be detected in the spore suspension mounted on the seed cavities of pea pod for 8 hours, but was recognized in the plot incubated for 12 hours, and increased with the length of incubation time. It reached the maximum when spore suspension was incubated for 20∼30 hours (Tables 1 and 2, Fig. 1).The activity of pea pod to produce the antibiotic factor was destroyed by either anesthetic treatment with ether (at a rate of 1.1cc ether in 4.71 volume of air, for 24 hours at 17∼22°C), or heat treatment at 40°C for 60∼90 minutes, before inoculation with spore suspension (Tables 3 and 4).
879nm-LD-pumped Nd:GdVO4 laser and its thermal property (2004)

An 879nm-LD-pumped Nd:GdVO4 laser with a maximum slope efficiency of 78% was realized. Optical-optical conversion efficiency reached 70%. We also measured the thermal conductivity and the thermal lens effect to characterize the thermal property of the Nd:GdVO4 laser.
Longitudinal qPCR Study of the Dynamics of L. crispatus, L. iners, A. vaginae, (Sialidase Positive) G. vaginalis, and P. bivia in the Vagina (2012)

This qPCR-based study confirms largely the results of previous culture-based, microscopy-based and pyrosequencing-based studies.
The effect of Roux-en-Y gastric bypass, sleeve gastrectomy and adjustable gastric banding on renal function and remission of metabolic disease: a five-year longitudinal study (2015)

Searchable abstracts of presentations at key conferences in endocrinology ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
Thinning of Retinal Nerve Fiber Layer Is Associated with Neurocognitive Impairment in HIV Infected Women (P01.263) (2012)

Objective: To evaluate if Optical Coherence Tomography (OCT) measurements of retinal nerve fiber layer (RNFL) correlate with diagnosis of HIV-associated Neurocognitive Disorders (HAND). Background HAND is associated with neuronal loss and eventual brain atrophy. In the era of combined antiretroviral therapy (CART) prior biomarkers of HAND are no longer useful. Since evidence supports that thinning of the (RNFL) as measured by OCT correlates with brain atrophy, axonal loss, and physical and cognitive disability in Multiple Sclerosis (MS) it is likely to be a good biomarker for HAND. Design/Methods: A pilot cross-sectional study nested in the longitudinal Hispanic/Latino Cohort of HIV-seropositive women. 30 women (15 HIV-seropositive, 5 with diagnosis of MS without history of optic neuritis, and 10 controls) were evaluated with neurological and neuropsychological exam. Cognition was determined using the modified AAN HIV dementia criteria. Only MS women with normal cognition were included. Among HIV-seropositive women, 7 had normal cognition/asymptomatic cognitive performance and 8 had symptomatic impairment (MCMD/HAD). OCT RNFL thickness average analysis test was performed in each of the subjects eyes. There was no significant difference in age and education among groups. OCT RNFL raw and normalized to the control group values were used. Statistics included non parametric and parametric associations and correlations. Results: Raw RNFL OCT values showed a significant association between the HIV-seropositive women without and with symptomatic impairment (p=0.002). Normalized RNFL OCT values confirm findings and demonstrated a significant association between HIV-seropositive without symptomatic impairment and those with MS (p Conclusions: This pilot study provides evidence for the use of OCT as a tool to evaluate brain dysfunction and neurodegeneration in HIV-seropositive women. This dysfunction may have a role in the diagnosis of HAND and could also represent a biomarker for HAND. Further studies should be performed to support current findings. Supported by: NIH: S11NS046278, U54NS43011, U54RR026139. Disclosure: Dr. De Jesus-Acosta has nothing to disclose. Dr. Rosado has nothing to disclose. Dr. Hechavarria has nothing to disclose. Dr. San Miguel has nothing to disclose. Dr. Serrano has nothing to disclose. Dr. Nath has nothing to disclose. Dr. Wojna has nothing to disclose.
Mitochondrial antioxidative capacity regulates muscle glucose uptake in the conscious mouse: effect of exercise and diet (2012)

The objective of this study was to test the hypothesis that exercise-stimulated muscle glucose uptake (MGU) is augmented by increasing mitochondrial reactive oxygen species (mtROS) scavenging capacity. This hypothesis was tested in genetically altered mice fed chow or a high-fat (HF) diet that accelerates mtROS formation. Mice overexpressing SOD2 (sod2(Tg)), mitochondria-targeted catalase (mcat(Tg)), and combined SOD2 and mCAT (mtAO) were used to increase mtROS scavenging. mtROS was assessed by the H(2)O(2) emitting potential (JH(2)O(2)) in muscle fibers. sod2(Tg) did not decrease JH(2)O(2) in chow-fed mice, but decreased JH(2)O(2) in HF-fed mice. mcat(Tg) and mtAO decreased JH(2)O(2) in both chow- and HF-fed mice. In parallel, the ratio of reduced to oxidized glutathione (GSH/GSSG) was unaltered in sod2(Tg) in chow-fed mice, but was increased in HF-fed sod2(Tg) and both chow- and HF-fed mcat(Tg) and mtAO. Nitrotyrosine, a marker of NO-dependent, reactive nitrogen species (RNS)-induced nitrative stress, was decreased in both chow- and HF-fed sod2(Tg), mcat(Tg), and mtAO mice. This effect was not changed with exercise. Kg, an index of MGU was assessed using 2-[(14)C]-deoxyglucose during exercise. In chow-fed mice, sod2(Tg), mcat(Tg), and mtAO increased exercise Kg compared with wild types. Exercise Kg was also augmented in HF-fed sod2(Tg) and mcat(Tg) mice but unchanged in HF-fed mtAO mice. In conclusion, mtROS scavenging is a key regulator of exercise-mediated MGU and this regulation depends on nutritional state.
Müllerian Duct Anomalies: Comparison of MRI Diagnosis and Clinical Diagnosis (2007)

Despite excellent agreement between MRI and clinical diagnoses of müllerian duct anomalies, there are discrepancies and pitfalls resulting mostly from the absence of a precise and integrated classification scheme, unfamiliarity with rare and complex entities, and suboptimal depiction of some structures on MRI.
Woronin Body Function in <i>Magnaporthe grisea</i> Is Essential for Efficient Pathogenesis and for Survival during Nitrogen Starvation Stress (2004)

The Woronin body is a peroxisome-derived dense-core vesicle that is specific to several genera of filamentous ascomycetes, where it has been shown to seal septal pores in response to cellular damage. The Hexagonal peroxisome (Hex1) protein was recently identified as a major constituent of the Woronin body and shown to be responsible for self-assembly of the dense core of this organelle. Using a mutation in the Magnaporthe grisea HEX1 ortholog, we define a dual and essential function for Woronin bodies during the pathogenic phase of the rice blast fungus. We show that the Woronin body is initially required for proper development and function of appressoria (infection structures) and subsequently necessary for survival of infectious fungal hyphae during invasive growth and host colonization. Fungal mycelia lacking HEX1 function were unable to survive nitrogen starvation in vitro, suggesting that in planta growth defects are a consequence of the mutant's inability to cope with nutritional stress. Thus, Woronin body function provides the blast fungus with an important defense against the antagonistic and nutrient-limiting environment encountered within the host plant.
Water sorption in thin films of high-temperature polyimides: The effect of imidization history (1994)

PMDA-ODA and BPDA-PDA polyimides in 10-50 Atrn thick films were prepared from the respective poly(amic acid) precursor by thermal imidization for 1-10 h at various temperatures over 230-400C. Their water sorption behaviours were studied in detail by gravimetry. Water sorption in both PMDA-ODA and BPDA-PDA followed a nearly Fickian process in spite of the morphological heterogeneity due to the ordered and less ordered phases. Water clustering or plasticization was very weakly evident on the water uptake versus humidity plots. Water sorption in both polyimides was highly dependent upon imidization history. Both diffusion coefficient and water uptake are decreasing functions of imidization temperature and time. This resulted mainly from the enhancement of overall crystallinity by high-temperature imidization because of their high-rT nature. The diffusion coefficient and water uptake were (1.0-3.0) x 10-9 cm2 s-1 and 0.4-4.5 wt% for PMDA-ODA and (0.9-3.0)x 10-cm2s-' and 0. I2-2.40wt%, depending on humidity,/i...
Pass’Mirail, dispositif de prévention des soins psychiques pour les 18-25 ans (2018)

Résumé Deux professionnels témoignent, après un an d’ouverture de Pass’Mirail, dispositif de prévention. Des jeunes de 18 à 25 ans en grande fragilité psychique y sont accueillis de façon anonyme et gratuite et participent à des temps d’échange et à des activités, dans un lieu atypique dont le cadre est pensé comme un non-cadre, au regard des pratiques sanitaires habituelles de ces professionnels. L’accent est mis sur une fonction et une clinique du groupe, accueilli et traité par un groupe de professionnels de quatre organismes différents qui ont mutualisé leurs moyens.
Crystal and molecular structure of .beta.-maltose octaacetate. A model in retrospect for amylose triacetate? (1982)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCrystal and molecular structure of .beta.-maltose octaacetate. A model in retrospect for amylose triacetate?F. Brisse, R. H. Marchessault, S. Perez, and P. ZugenmaierCite this: J. Am. Chem. Soc. 1982, 104, 26, 7470–7476Publication Date (Print):December 1, 1982Publication History Published online1 May 2002Published inissue 1 December 1982https://pubs.acs.org/doi/10.1021/ja00390a013https://doi.org/10.1021/ja00390a013research-articleACS PublicationsRequest reuse permissionsArticle Views104Altmetric-Citations29LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Clinical Pharmacodynamics of High-Dose Methotrexate in Acute Lymphocytic Leukemia (1986)

As the intensity of cancer chemotherapy has been reported to influence clinical response for several drug-sensitive cancers, we have investigated the relation between systemic exposure to high-dose methotrexate (HDMTX) and clinical response in childhood acute lymphocytic leukemia (ALL). A total of 108 consecutive, previously untreated children with "standard-intermediate risk" ALL were randomized to receive postinduction therapy with HDMTX (1000 mg/m2 iv over 24 hours weekly for 3 weeks, then every 6 weeks for 72 weeks), superimposed on conventional therapy with low-dose 6-mercaptopurine (6MP; 50 mg/m2 orally per day) and methotrexate (MTX; 25 mg/m2 orally per week). The systemic clearance of HDMTX ranged from 40 to 131 ml/minute/m2 among these patients, yielding MTX steady-state plasma concentrations (Cpss) ranging from 9.3 to 25.4 microM during the infusion. The group of patients (n = 59) with median MTX Cpss less than 16 microM during the HDMTX infusion had a higher probability of having any relapse than patients (n = 49) with MTX Cpss greater than 16 microM (P less than 0.05). In a previously reported univariate analysis, patients with MTX Cpss less than or equal to 16 microM were 3.2 times more likely to relapse on therapy (P = 0.01) and 6.9 times more likely to have a hematologic relapse on therapy (P = 0.001). Multivariate and stepwise Cox's regression analyses indicated that MTX Cpss retains its prognostic importance even when other prognostic variables (i.e., DNA Index, WBC, hemoglobin) are considered.(ABSTRACT TRUNCATED AT 250 WORDS)
Synthesis and characterization of new methacrylate based hydrogels (2006)

Hydrogels have been used for several applications, including production of contact lenses, sanitary products and materials for wound dressing. The aim of this work was the development of new methacrylate based hydrogels. These materials present the advantage of being easily produced with different compositions and consequently different permeability and diffusion patterns. Therefore it becomes possible to synthesize hydrogels that can be used to immobilize a variety of compounds, such as drugs, proteins or even cells. During this work new polymers based on methacrylate monomers were prepared. Their characterization was accomplished by several techniques, e.g. Fourier Transform Infrared Spectroscopy (FTIR), swelling measurement, Differential Scanning Calorimetry (DSC) and evaluation of mechanical properties. Their subacute subcutaneous toxicity was also evaluated by using Wistar rats.
Role of Pili in Bacterial Conjugation (1970)

We describe techniques for isolating individual pairs of mating Escherichia coli and observing them under the light microscope. Some pairs achieved close cell-to-cell contact, whereas others remained loosely connected by invisible connections which may be F pili. After 30 min of mating, the pairs were separated and allowed to grow into clones. That many exconjugants derived from "loose"-mating pairs produced recombinants suggests that F pili are involved in the transfer of genetic material. The frequency of formation of recombinants from "close"-mating pairs, however, was significantly higher than that from loose-mating pairs, indicating that a close cell-to-cell contact facilitates chromosome transfer. Death rates of exconjugants from close pairs were also higher than those from loose pairs. Hfr x F(-) matings produced higher death rates than F(+) x F(-) matings. Male cells were found capable of transferring genetic markers to two F(-) cells simultaneously. We conclude that F pili play at least three roles in mating: (i) they initiate contacts between mating pairs; (ii) they facilitate the transfer of genetic material; and (iii) they draw mating cells into a close contact which increases the fertility of the union.
Factor VIII-Associated Platelet Aggregation (1977)

* Recipient of a Research Career Development Award #HL 00275. This work was supported by the National Institutes of Health, grants #HL 15474 and HL14217.