gebhart/patrepeval huggingface dataset:
Probe-free label system for rapid detection of Cronobacter genus in powdered infant formula (2018)

Cronobacter species previously known as Enterobacter sakazakii poses high risks to neonates and infants. In this work a rapid detection method was developed which combined loop-mediated isothermal amplification with lateral flow assay for detection of Cronobacter species in powdered infant formula. The fast amplification reaction without betaine was established and capable of performing DNA replication within 25 min. Based on the novel probe-free labeling methods, we established a lateral flow assay to capture the specific loop-mediated isothermal amplification amplicons which were labeled with fluorescein isothiocyanate and biotin. And the final detection time of this system was within 40 min. The false positive results of the lateral flow assay induced by primer dimer tagged with fluorescein isothiocyanate and biotin were eliminated by Taq single strand DNA binding protein (4 ng/μL). Simultaneously, the efficiency of the fast loop-mediated isothermal amplification assay was achieved. By injection of Taq SSB into the amplification assay as a replacement for betaine, the novel probe-free method could detect Cronobacter species with high specificity and sensitivity at the detection limit in PIF of 10<sup>1</sup> cfu/g. Our overall strategy has excellent potential in the rapid diagnosis of Cronobacter species label-free by integrating loop-mediated isothermal amplification and lateral flow assay.
Electromechanical synchronization of the heterotopic and native heart by dual atrial stimulation following heart transplantation (2006)

After heterotopic heart transplantation, a 59-year-old woman presented with remarkable symptoms of breathlessness and fatigue, despite excellent donor heart function. Asynchrony of donor and native heart provoked haemodynamic instability. Dual atrial pacemaker implantation lead to linkage and synchronization of atrial and ventricular contraction in both the donor and native heart with the faster organ executing the synchronization. Remarkable relief of symptoms has been evident during the long-term follow-up.
QED: Securing the Mobile Masses (2003)

The growing trend of nomadic and mobile computing raises serious security concerns. Nomadicity has become common; users are in constant transit, migrating with their mobile devices through many diverse wireless environments. Some users are innocent—potentially vulnerable, but benign—while others are malicious or carry compromised devices. The mobile commons brings all of these users and devices together. Both clients and hosting environments must be protected. To that end, we are developing QED, a new security model for computer networks. QED enables environments to quarantine devices, examine them, and potentially update or “decontaminate ” client nodes. We present the design of our QED prototype and evaluate its performance in our laboratory. 1.
ChemInform Abstract: DIRECTION OF RING OPENING OF 2‐METHYL‐SUBSTITUTED CYCLOPROPYL(STANNYLOXY)METHYL AND CYCLOPROPYL(HYDROXY)METHYL RADICALS‐ KINETIC AND THERMODYNAMIC CONTROL (1977)

Abstract Durch Photolyse von (I) in Gegenwart von Di‐tert.‐butylperoxid bei ‐67 bis 112°C werden die Radikale (II) hergestellt, von denen die trans‐Verbindungen zu den thermisch weniger stabilen prim. Alkylradikalen (III) und die cis‐Verbindungen zu den sek. Alkylradikalen (IV) führen.
Proton radiation therapy for primary sphenoid sinus malignancies: Treatment outcome and prognostic factors (2009)

Proton radiation therapy results in excellent local control in patients with advanced primary sphenoid sinus malignancy. Brain invasion, involvement of the oropharynx and anterior cranial fossa are important prognostic factors.
Major histocompatibility complex-restricted H-Y-specific antibodies and cytotoxic T lymphocytes may recognize different self determinants. (1982)

Previous studies have shown that influenza virus-immune cytotoxic T lymphocytes can recognize virus in conjunction with self HLA-A2 antigens. Nevertheless, the virus-infected target cells from one HLA-A2-positive male donor (designated M7) could not be lysed by the virus-immune cytotoxic lymphocytes from any HLA-A2-matched unrelated donors. Although extensive serological analyses showed no difference between the HLA-A2 antigens of donor M7 and other HLA-A2-positive donors, isoelectric focusing of the HLA-A2 molecule from donor M7 revealed a clear difference in the heavy polypeptide chains when compared with the HLA-A2 molecules of other donors. The present study demonstrates that the HLA-A2-restricted anti-H-Y cytotoxic T lymphocytes obtained from a female aplastic anaemia patient fail to lyse the male M7 target cells, whereas the HLA-A2-restricted anti-H-Y antibodies from the same patient react with the cells of donor M7. These results suggest that: (a) HLA-A2-restricted anti-H-Y antibodies can recognize self determinants on the HLA-A2 molecule that are distinct from those that are recognized by HLA-A2-restricted anti-H-Y cytotoxic T cells; and (b) HLA-restricted T and B cells may use different receptor repertoires for the recognition of foreign antigens such as H-Y.
Cadmium accumulation in populations of <i>Thlaspi caerulescens</i> and <i>Thlaspi goesingense</i> (2000)

The capacity to accumulate cadmium (Cd) and zinc (Zn) was compared in Thlaspi goesingense and four populations of Thlaspi caerulescens . Two populations of T. caerulescens were grown in hydroponics at five concentrations of Cd. In addition, plants were grown in pots containing compost in which three different concentrations of Cd and two concentrations of Zn were added. A field trial was conducted to compare Zn and Cd uptake by three populations of T. caerulescens on nine selected plots of the Woburn Market Garden Experiment (UK) which had been contaminated to different degrees with heavy metals owing to past applications of sewage sludge. Results show that the four populations of T. caerulescens had the same ability to hyperaccumulate Zn but were significantly different in terms of Cd accumulation. Two populations of T. caerulescens from Southern France accumulated much more Cd than the populations from Prayon (Belgium) and Whitesike (UK). Generally, uptake of Cd was not decreased by increased concentrations of Zn in the substrate. These results indicate that the mechanisms of Cd and Zn hyperaccumulation are not identical in this species. This is the first report of hyperaccumulation of Cd by T. goesingense , but the growth of this species was markedly reduced by the large concentrations of Zn in the substrate. Future work should focus on the differences between Cd and Zn uptake in hyperaccumulator plants at the species and population level.
The distribution of calibrated likelihood-ratios in speaker recognition (2013)

This paper studies properties of the score distributions of calibrated log-likelihood-ratios that are used in automatic speaker recognition. We derive the essential condition for calibration that the log likelihood ratio of the log-likelihood-ratio is the log-likelihood-ratio. We then investigate what the consequence of this condition is to the probability density functions (PDFs) of the log-likelihood-ratio score. We show that if the PDF of the non-target distribution is Gaussian, then the PDF of the target distribution must be Gaussian as well. The means and variances of these two PDFs are interrelated, and determined completely by the discrimination performance of the recognizer characterized by the equal error rate. These relations allow for a new way of computing the offset and scaling parameters for linear calibration, and we derive closed-form expressions for these and show that for modern i-vector systems with PLDA scoring this leads to good calibration, comparable to traditional logistic regression, over a wide range of system performance.
Operating Plan for Continuous Kneading of Electrically Conductive Resin with Analysis of Time Series. (1993)

連続混練において一定品質の製品を連続的に生産するには, 主プロセスである混練機の動特性を考慮しなければならない.すなわち低周波域における入出力間での相関性を調べ, その結果に基づいて原料の供給速度などを制御する必要がある.本研究では, 原料供給速度および材料温度の変動を連続混練装置への入力, 流動性の指標である混練トルクを出力として解析した.その結果, 入出力の相関は低周波領域で顕著であった.また, 供給速度および混練温度の低周波数変動が高周波域に比較して大きな値を示すことがわかった.すなわち, 低周波の変動を抑えることにより安定な状態で混練を行うことが可能であることが確認された.これらの結果・考察より, 原料の供給器を制御すれば, 導電性樹脂の導電性を安定させて連続的に得られることを示した.また, 制御の効果は, 押し出し流れに近くなる低供給速度の領域において顕著であることを指摘した.
Asymmetric Cleavage of Racemic 1,3‐Oxazolidines – A New Dynamic Process in Homogeneous Rh(I)‐Catalyzed Hydrogenation (2003)

Abstract The mechanism of the homogeneously catalyzed hydrogenation of 1,3‐oxazolidines with a Rh(I) diphosphane catalyst affording tertiary hydroxyethyl‐substituted amines was investigated with the help of D 2 labelling experiments. It was found that the reaction proceeds via prochiral intermediates with, preferentially, an iminium cation being in equilibrium with the 1,3‐oxazolidine. This observation opened up the opportunity to run the reaction stereoselectively by employment of a racemic 1,3‐oxazolidine and a chiral catalyst. By means of high‐throughput screening considering 144 catalysts a cationic Rh(NORPHOS)‐complex was identified as the most efficient catalyst, which afforded after optimization of solvent, temperature and H 2 pressure the corresponding chiral hydroxyethylamine in 95% yield and 80% ee.
Étiologie moléculaire des rachitismes vitamino-dépendants héréditaires (2001)

La vitamine D subit deux transformations métaboliques conduisant à la synthèse de sa forme hormonale, la 1,25-dihydroxyvitamine D. Des mutations dans le gène du cytochrome P450, 25-hydroxyvitamine D-1-α-hydroxylase (1α-OHase), sont responsables d’une forme de rachitisme vitamino-dépendant héréditaire, nommée PDDR <i>(pseudovitamin D deficiency rickets)<i/> . Vingt-six mutations différentes, incluant des duplications et des délétions modifiant le cadre de lecture, et des mutations ponctuelles affectant la séquence de la protéine, l’épissage, ou conduisant à l’arrêt prématuré de la traduction, ont été détectées chez les patients de PDDR. Nous avons créé un modèle animal de PDDR en inactivant le gène <i>1<i/> <i>α<i/> <i>-OHase<i/> chez des souris. Cet article présente le phénotype des souris mutantes, résume l’état actuel des connaissances concernant l’expression et la fonction de la <i>1<i/>α <i>OHase<i/> , et discute des nouvelles perspectives de recherche sur le métabolisme de la vitamine D.
PER-1 Extended-Spectrum β-Lactamase Production in an <i>Alcaligenes faecalis</i> Clinical Isolate Resistant to Expanded-Spectrum Cephalosporins and Monobactams from a Hospital in Northern Italy (2000)

An Alicaligenes faecalis (FL-424/98) resistant to expanded-spectrum cephalosporins and aztreonam was isolated from the urine of an inpatient at the Intensive Care Unit of the Varese Hospital (Northern Italy) after antimicrobial chemotherapy with cefazolin, vancomycin, and amikacin. Clavulanic acid restored the activity of expanded-spectrum cephalosporins, suggesting the production of an extended-spectrum beta-lactamase (ESbetaL). A crude extract of FL-424/98 showed the presence of two beta-lactamase activities focusing at pH 5.3 and 7.6, respectively. The ESbetaL activity, purified by means of three chromatographic steps, was found to correspond to the pI 5.3 enzyme. Determination of kinetic parameters confirmed that the enzyme efficiently hydrolyzed expanded-spectrum cephalosporins and aztreonam. A colony-blot hybridization revealed the presence of blaPER-related sequences in FL-424/98, and sequencing confirmed the identity of this determinant with blaPER-1, previously detected in Pseudomonas aeruginosa, Acinetobacter, and Salmonella clinical isolates from Turkey. Finding of blaPER-1 in a species that can be part of the resident human microbiota raises the possibility that it could be an efficient shuttle for spreading of this resistance gene among other opportunistic pathogens that are normally members of the resident microbiota. Kinetic parameters determined for the PER-1 enzyme with some cephalosporin substrates were somewhat different from those previously reported.
Regression of Latent Endometrial Precancers by Progestin Infiltrated Intrauterine Device (2006)

PTEN tumor suppressor inactivation is the earliest step in endometrial carcinogenesis, occurring in morphologically unremarkable endometrial glands in half of normal women. We test the hypothesis that sex hormones positively or negatively select for these "latent precancers" by examining their emergence, persistence, and regression rates under differing hormonal conditions. Perimenopausal and postmenopausal women had an intake endometrial biopsy and underwent hormonal therapy with progestin-impregnated intrauterine device (IUD; n = 21), cyclic oral progestins (n = 28), or surveillance only (n = 22) with follow-up biopsies. For comparison, premenopausal naturally cycling endometrial biopsies were studied as single time points in 87 patients and multiple surveillance time points in 34 patients. Biopsies in which any PTEN protein-null glands were found by immunohistochemistry were scored as containing a latent endometrial precancer. All groups had a similar proportion of latent precancers at intake but differed after therapy. Emergence rates were highest (21%) for the naturally cycling premenopausal group compared with just 9% for untreated perimenopausal women. The IUD group had the highest rate of regression, with a 62% pretherapy and 5% post-therapy rate of latent precancers. This contrasted to nonsignificant changes for the oral progestin and untreated control groups. Delivery of high doses of progestins locally to the endometrium by IUD leads to ablation of preexisting PTEN-inactivated endometrial latent precancers and is a possible mechanism for reduction of long-term endometrial cancer risk known to occur in response to this hormone.
GROWING SURFACE WAVES IN A SEMICONDUCTOR IN THE PRESENCE OF A TRANSVERSE MAGNETIC FIELD (1968)

Recent observations of growing carrier waves in InSb suggest a new type of interaction involving electrons and holes which leads to a growing surface wave. A theory is presented which shows that the wave has a phase velocity near the electron drift velocity, and in the presence of a transverse magnetic field is unstable with very large growth rates.
Planing Surface C sub f (1983)

For model-full scale resistance estimates the C sub f value from the ATTC/ITTC line is used to scale the frictional resistance in calm water resistance tests of planning hulls. In this contribution C sub f values from published data on planing plates, prismatic hulls, and Series 62 models are compared to the corresponding ATTC C sub f values. Here 0.003 C sub f sub 1 0.004 which is below the ATTC line Rn 2 million and above the ATTC line for higher Rn. While the differences are within engineering accuracy + or - 10% of the ATCC line, the force vector analysis of the planing test data is appropriate to verify the extent of turbulent flow on the planing surface.
Compensation for Nonlinear Distortion in OFDM Systems Using a Digital Predistorter Based on the SCPWL Model (2010)

Orthogonal frequency division multiplexing (OFDM) is an attractive technique for achieving high-bit-rate wireless data transmission. However, multicarrier systems such as OFDM show great sensitivity to nonlinear distortion. In this paper, we propose a simplicial canonical piecewise-linear (SCPWL) model based digital predistorter to compensate for nonlinear distortion introduced by a high power amplifier (HPA) in OFDM systems. The performance of the new predistortion scheme for OFDM systems is evaluated in terms of bit error rate (BER) and total degradation (TD). The simulation results demonstrated that the proposed predistorter achieves significant performance improvement by effectively compensating for the nonlinear distortion introduced by the HPA.
Preparticipation Sports Evaluation: Issues for Healthy Children and Athletes With Disabilities (2010)

Athletic participation in youth sports is occurring on an ever-increasing level each year. All states require an examination before young athletes are allowed to participate in interscholastic sports to identify conditions that may increase the risk of injury. The aims of a preparticipation sports evaluation are to detect conditions that might be life-threatening, disabling, or that may predispose to injury; to indicate which sports are safe for the individual; to serve as a limited general health screening and to meet legal requirements. Preparticipation sports evaluation therefore plays a crucial in ensuring the health and safety of the disabled and healthy young athlete.
A Comparison of<sup>18</sup>F and<sup>85</sup>Sr Scintimetric Patterns by Computerized Data Analysis and Display (1973)

Forty-nine patients underwent scintimetry of 104 hip and knee joints for evaluation of benign disorders 1–2.5 hours after intravenous injection of 1 mCi 18F, then received 100μCi 85Sr and were rescanned in two weeks. To equate the high counting values obtained following 18F to the lower counts following 85Sr, a new system of computerized data analysis and oscilloscope display was devised. In 100 of the 104 joints scanned, 18F and 85Sr gave strikingly similar distribution patterns. In 3 of the remaining 4 joints, elevated counts compatible with the primary disorder were found with both agents but the extent of elevation pattern differed.
Atrioventricular Nodal Reentrant Paroxysmal Supraventricular Tachycardia (1984)

Paroxysmal supraventricular tachycardia (PSVT) is commonly encountered in the clinical practice. The typical patient with this disorder is young and experiences sudden onset of palpitation, chest discomfort, or dizziness of various frequency and duration. The rate of the tachycardia is regular (130 to 250 beats per minute). The termination of the paroxysm is abrupt and can occur spontaneously; otherwise the tachycardia is terminated by vagal maneuvers, eg, carotid sinus massage, pharmacologic interventions, electrical pacing, or direct current countershock. In the majority of cases, the mechanism of PSVT is circus movement of the cardiac impulse.<sup>1</sup>Reentrant excitation causing this disorder develops either between the sinus node and the right atrium or within the atria or the atrioventricular node. In a fourth common type of PSVT, the reentry loop incorporates the atria, the atrioventricular node, the ventricular myocardium, and an accessory tract between ventricles and atria. The various forms of reentrant
Mechanism and kinetics of the reaction between sulfur dioxide and ammonia in flue gas (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMechanism and kinetics of the reaction between sulfur dioxide and ammonia in flue gasKlaus Hjuler and Kim Dam-JohansenCite this: Ind. Eng. Chem. Res. 1992, 31, 9, 2110–2118Publication Date (Print):September 1, 1992Publication History Published online1 May 2002Published inissue 1 September 1992https://pubs.acs.org/doi/10.1021/ie00009a007https://doi.org/10.1021/ie00009a007research-articleACS PublicationsRequest reuse permissionsArticle Views428Altmetric-Citations10LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Quasi non-diffractive electron Bessel beams using direct phase masks with applications in electron microscopy (2019)

Electron-beam shaping opens up novel imaging possibilities in electron microscopy (EM). The implementation of a phase or amplitude mask in the condenser lens system allows the generation of electron beams with various shapes. Non-diffractive Bessel beams (BBs) are of interest for numerous applications due to their extraordinary large depth of focus. We present an experimental demonstration of single high-intensity quasi non-diffractive electron BBs generated by direct phase masks (PMs). The PM fabrication by focused ion beam is optimized using custom scan routines. The propagation of the electron beam after transmission through several PMs is analyzed in detail. A sub nm-sized non-diffractive electron beam is realized in the object plane of a transmission electron microscope. The experiments agree well with simulations and a route towards a beneficial application of non-diffractive electron beams in EM is discussed.
Characterization of advanced excimer laser crystallized polysilicon thin film transistors (2004)

This work presents electrical and radiation stress as well as transient current analysis results for thin film transistors fabricated using an advanced polysilicon laser crystallization method, termed sequential lateral solidification. The TFTs exhibit excellent device characteristics and parameters such as very high electron mobility, as well as good bias stress endurance, with only a small threshold shift but without appreciable subthreshold swing degradation. The analysis of the drain current transients observed after a gate bias pulsing in the OFF state indicated a peak in their temperature dependence, with a generation freezeout at cryogenic temperatures suggesting good crystal quality. Gamma irradiation of TFTs, particularly in the presence of gate bias, resulted in a negative threshold voltage shift due to positive oxide charge creation and a small subthreshold swing degradation.
Molecular cytogenetic and clinical studies of 42 patients with marker chromosomes (1992)

The molecular cytogenetic characterization and clinical details of 20 patients with marker chromosomes are presented. These 20 patients, together with another 22 patients previously published, represent a cohort in which the chromosomal origin of the marker chromosomes was successfully determined in all but one case. Examination of the pooled data suggests that the satellited markers derived from chromosomes 14, 15 (when metacentric or submetacentric), those whose origin is either 13 or 21, and those small ring autosomal markers derived from both alphoid and satellite II or III pericentric heterochromatin of chromosomes 1, 9, 15, and 16 are all associated with a low risk of phenotypic abnormality. The markers identified as i(18p), ring chromosomes derived from various autosomes, and satellited markers derived from chromosome 22 are associated with a high risk of phenotypic abnormality. The phenotype of patients with acrocentric markers derived from chromosome 15 was equivocal, perhaps as a result of imprinting. Additional data are required to confirm these trends. The mild mental retardation and abnormal face of a patient with a small ring chromosome derived from chromosome 4 are described. Identification of patients with small rings originating from particular chromosomes may allow the recognition of new syndromes.
F. Die anwaltliche Tätigkeit in der Verwaltungsvollstreckung (2014)

In den letzten Jahren wurde innerhalb der Referendarausbildung das Gewicht verstärkt auf die anwaltliche Tätigkeit gerichtet, was auch auf die Klausuren im Assessorexamen durchschlägt. Die Neuerscheinung macht die Rechtsreferendare mit den Anforderungen bei der Lösung einer Anwaltsklausur im Öffentlichen Recht vertraut.