gebhart/patrepeval huggingface dataset:
T7 RNA Polymerase Mediates Fast Promoter-Independent Extension of Unstable Nucleic Acid Complexes (2004)

T7 RNA polymerase is a processive, DNA-dependent RNA polymerase that has a high specificity for its 17 base pair (bp) promoter. In addition to normal transcription, the enzyme can produce anomalous transcripts in the absence of a promoter. We report here the systematic characterization of the transient aspects of this promoter-independent process. Oligonucleotides that are able to form transient unimolecular loop structures closed by as little as one Watson-Crick base pair between the 3' terminal residue and an internal nucleotide proved to be viable substrates. A single nucleotide triphosphate assay system found that incorporation was encoded by the nucleotide 5' to the predicted transient base pair. When this coding nucleotide was identical to the internal nucleotide participating in the transient base pair, multiple nucleotide incorporations were observed and could only be explained by a continuous shifting and resetting of the transient base-pairing required for extension. This intermittent extension process can be quite efficient. Short DNA or RNA substrates were good substrates for the enzyme (affinities ranged from 2 to 43 microM) and were extended rapidly with apparent catalytic rates of up to 240 min(-1) being observed, only 2-fold slower than the rate of transcriptional initiation. Our data suggest a possible mechanism for this promoter-independent extension activity and may add to the understanding of viral RNA replicative strategies.
Parametric Study of Sand-Production Prediction: Analytical Approach (1989)

Summary An unconventional perforation-tunnel stability analysis is conducted in this work by separating the role of two independent factors on sand production: well pressure and local pressure gradient around a cavity. Such a separation clarifies most of the phenomena observed during sand production in oil fields. Simple analytical solutions for poroelastoplastic materials allowed repeated calculations for a parameter sensitivity study. The results obtained should be used qualitatively because of some degree of simplification.
ChemInform Abstract: AZETIDINONE ANTIBIOTICS. 22. A REARRANGEMENT OF OXOAZETIDINESULFENIC ACIDS TO O HALOAZETIDINONES (1982)

Abstract Mit N‐Chlorsuccinimid (NCS) liefert Penicillin‐sulfoxid (I) das Sulfinylchlorid (IIa), das zur Säure (IIb) hydrolysiert wird.
Distinct features of SARS-CoV-2-specific IgA response in COVID-19 patients (2020)

<b>Humoral immune response to SARS-CoV-2 showed an early response of IgA, instead of IgM, in COVID-19 patients. As highlighted by this study, enhanced IgA responses observed in severe COVID-19 might confer damaging effects in severe COVID-19.</b>https://bit.ly/3fA7c1I
Demonstration and performance of a recirculating sorter based on complementary metal oxide semiconductor logic and vertical cavity surface emitting lasers (1998)

The demonstration of an optoelectronic sorter unit is pre- sented. This sorter is constructed using a recirculating architecture that passes data optically between two smart-pixel arrays. Each array is implemented using a hybrid combination of CMOS logic and vertical cav- ity surface emitting lasers (VCSELs). Since implementation of this sys- tem is intended as a tool for evaluating the technological issues associ- ated with CMOS/VCSEL smart-pixel device, the system implementation is designed to minimize interconnect complexity while focusing on the smart-pixel design and analysis. A power budget analysis indicates that the photoreceivers require a minimum of 130 mW of optical input power, which leads to a VCSEL power requirement of '415 mW. The fre- quency response of the photoreceivers and logic circuit reveals that the photodetector limits the operation to '3 MHz, which is an order of mag- nitude slower than the logic circuits frequency limit. © 1998 Society of Photo- Optical Instrumentation Engineers. (S0091-3286(98)03101-8)
Synthesis of a video of performers appearing to play user-specified band music (2012)

We propose a novel method to synthesize a video of multiple performers appearing to play the user-specified band music. The performance video often helps us to enjoy or understand the music. For example, watching the performance of a bass player makes it easier for us to focus on the bass sound, which is an experience different from listening to the music just using speakers or headphones. Our approach is based on the database of performance videos: given the band music, our system chooses appropriate footages from the database, slightly modifies their speeds and timings according to the music, and then concatenates them as the resulting video. In existing research, there are several methods proposed for synchronizing videos to a user-specified music, e.g., to synthesize a dance video [Nakano et al. 2011]. These methods synchronize a video to the music by analyzing its mood based on tempo or chord changes in the music. On the other hand, since we want to synthesize a performance video and require more precise synchronization, we analyze the music extracting the timings of musical notes from the audio signal (Fig.1). We perform best match search using the timings as feature vector, and copy the footage that has a similar set of timings (Fig.1-a and b). We demonstrate that our method enables to create a performance video of a band music, which is a fake but looks interesting.
Production of Goat, Rat, and Guinea Pig Antisera to Murine IgE (1976)

Abstract The study of immunoglobulins (Ig)3 has been facilitated by the production of specific anti-Ig sera (1–4). For each of the six of the known classes and subclasses of mouse Ig, there exists a myeloma synthesizing the corresponding paraprotein, which has been used for the preparation of specific antiserum. Myeloma proteins have also been used to study the IgE class of antibodies in man (5) and in the rat (6), but to date no mouse IgE (IgEm) myeloma has been discovered. Nevertheless, one report has been published in which it was claimed that rat anti-mouse IgEm was produced (7) by immunization of Lewis rats with syngeneic peritoneal cells coated with IgEm; the mouse reagin has been adsorbed onto these cells by incubating them with mouse reaginic serum (MRS). However, in spite of extensive efforts, this procedure could not be reproduced in this laboratory. In the present report are described alternative methods that have resulted in the production of anti-IgEm in three animal species, viz., the rat, goat, and guinea pig.
Pseudophakic posterior iris chafing syndrome (1986)

Posterior iris chafing by the loop or the optic portion of sulcusfixated posterior chamber lens implants may cause a spectrum of disorders that include iris-pigment epithelial "window defects," pigment dispersion with or without elevation of intraocular pressure, intermittent microhyphemas with transient visual obscurations, and the UGH syndrome. It appears that secondary pigmentary glaucoma is more likely with planar loop design than with angulated loops. Optic and loop materials may play a role in the development of the disorder. Implantation of both supporting loops of the implant within the capsular bag is suggested to prevent posterior iris chafing.
Gene Transfer of Human Prostacyclin Synthase Ameliorates Monocrotaline-Induced Pulmonary Hypertension in Rats (2000)

Intratracheal transfer of the human PGIS gene augmented pulmonary prostacyclin synthesis, ameliorated MCT-induced pulmonary hypertension, and thereby improved survival in MCT rats.
Loci controlling lymphocyte production of interferon γ after alloantigen stimulation in vitro and their co-localization with genes controlling lymphocyte infiltration of tumors and tumor susceptibility (2009)

Low infiltration of lymphocytes into cancers is associated with poor prognosis, but the reasons why some patients exhibit a low and others a high infiltration of tumors are unknown. Previously we mapped four loci (Lynf1–Lynf4) controlling lymphocyte infiltration of mouse lung tumors. These loci do not encode any of the molecules that are involved in traffic of lymphocytes. Here we report a genetic relationship between these loci and the control of production of IFNγ in allogeneic mixed lymphocyte cultures (MLC). We found that IFNγ production by lymphocytes of O20/A mice is lower than by lymphocytes of OcB-9/Dem mice (both H2pz) stimulated in MLC by irradiated splenocytes of C57BL/10SnPh (H2b) or BALB/ cHeA (H2d) mice, or by ConA. IFNγ production in MLCs of individual (O20 9 OcB-9)F2mice stimulated by irradiated C57BL/10 splenocytes and genotyped for microsatellite markers revealed four IFNγ-controlling loci (Cypr4-Cypr7), each of which is closely linked with one of the four Lynf loci and with a cluster of susceptibility genes for different tumors. This suggests that inherited differences in certain lymphocyte responses may modify their propensity to infiltrate tumors and their capacity to affect tumor growth.
Posibilidades de las tareas de expresión corporal para suscitar emociones en el alumnado (2011)

Actualmente existe un creciente interés por la influencia de las emociones en el rendimiento deportivo, así como en la actividad física relacionada con el bienestar. El objetivo de este estudio fue analizar las respuestas emocionales de estudiantes durante la práctica de Expresión Corporal y observar la influencia del tipo de tarea. 80 estudiantes de primer curso de Ciencias de la Actividad Física y el Deporte participaron en 14 sesiones de 50 minutos de Expresión Corporal. Escribieron un diario después de cada sesión, en el que se recogían las emociones y sensaciones experimentadas en cada tarea. Tres investigadoras los analizaron, clasificaron las emociones y las relacionaron con el tipo de tareas. Los resultados muestran que la práctica de la Expresión Corporal provoca eminentemente comentarios relacionados con emociones positivas y que el tipo de tarea parece condicionar las emociones que se desencadenan.
Modification and Testing of Advanced Hydraulic Cutting Tools for Use in an Arctic Environment (2001)

Abstract An assessment was performed of Russian needs and capabilities related to decommissioning wastes from nuclear submarines. Hydraulically operated hand-held metal cutting tools can provide improved productivity and safety during the size and volume reduction of bulky metal scrap. Such tools are commercially available in the US, but not in Russia. Also, they have not been previously deployed in Arctic conditions. A system of metal cutting and spreading tools has been procured, modified and successfully tested at the Cold Regions Research and Engineering Laboratory (CRREL), and are now being deployed to Russia’s Northern Fleet.
Development of supermolecular structure in polystyrene latexes (1989)

Abstract The development of supermolecular structure in polystyrene latex particles was investigated by small‐angle neutron scattering (SANS). Seed latexes of deuterated polystyrene were swollen with an equal mole‐% of protonated styrene monomer, equilibrated, and polymerized to complete conversion. The scattering patterns reflect the extent of supermolecular structure development inside the particle. The data were analyzed by separating the Debye single‐chain and spherical scattering form factors. The extent of segregation was found to depend on the relative size of the chains compared with that of the particle, going through a maximum at the ratio M w / D of about 0.1, where D w represents the weight‐average diameter of the latex particle. When the chain dimensions are relatively very small, uniform molecular mixing is obtained. The best model for the segregated system is a graduated core‐shell supermolecular structure.
Development of an Evaluation Method for Controlling Skill of a Myoelectric Control Hand (2008)

The aim of this study was to develop a new evaluation method for controlling skill of a myoelectric control hand (MCH). Four amputees who daily used the MCH participated in this study. Measurement of two signals which were needed to control motion of the MCH was made. The two signals were assigned average rectified electromyogram of forearm extensors and flexors, respectively. The values of the signals increased depending on the muscles contraction level. Therapy for using the MCH was done to improve the ability of separate contractions of two muscles. This ability was defined as the skill to control motion of the MCH. The skill in each motion was evaluated by regression coefficient which was calculated by the least squares method. Regression coefficient was in inverse proportion to the skill. The quantitative index of the skill would be provide by developed method, and should be useful for efficient therapy to improve the controlling skill of myoelectric control hand.
Identification of a new phase in the Ai-C-B ternary by high-resolution transmission electron microscopy (1987)

The purpose of this note is to report the identification of a ternary (XI) phase which has been observed as a reaction product during the processing of B 4 C-Al composites. These ceramic-metal composites (cermets) have the unique potential for structural applications because of their low density (> 2.6 g/cc) and a desirable combination of mechanical properties. Guidelines for the processing of B 4 C-Al have been successfully established; and now it is possible to produce tailored microstructures with varying amounts of constituent phases. The samples for the analysis were prepared by the following procedure. First, it was necessary to heat the B 4 C powder compacts in excess of 2000°C to attain an interconnected B 4 C skeletal structure. Liquid aluminum was then infiltrated into this B 4 C network at 1170°C.
Development of a liquid crystal with fine‐pitch light‐source display (LFD) using an organic light‐emitting diode (OLED) (2006)

Abstract— A novel display system, refered to as an LFD (liquid crystal with fine‐pitch light‐source display) is proposed. In an LFD, an auxiliary light source patterned with a fine pitch is attached to a reflective liquid‐crystal display (LCD), and a light shield is formed on the observer's side of the light source. A vertical‐alignment LCD (VA‐LCD) is attached as the reflective LCD, and an organic light‐emitting diode (OLED) is attached as the fine‐pitch light source. An LFD can produce bright, high‐contrast images under any ambient light. A test sample was built and its display characteristics confirmed.
Late-onset hypogonadism in men with systolic heart failure: prevalence, clinical associates, and impact on long-term survival (2014)

Background Male ageing is characterized by diminished circulating androgens with several adverse psychosomatic consequences and can be aggravated by concomitant chronic diseases. According to the European Male Aging Study (EMAS) Group, late-onset hypogonadism (LOH) refers to testosterone deficiency accompanied by sexual complaints. Aim We investigated the prevalence of LOH in men with systolic heart failure (HF), and its clinical determinants and prognostic consequences. Methods Among 201 men with systolic HF (age: 60 ± 11 years), serum total testosterone (TT) was assessed using an immunoassay, and estimated free testosterone (eFT) was calculated using Vermeulen's formula. LOH was diagnosed when TT < 3.2 ng/mL and eFT < 64 pg/mL were accompanied by three sexual symptoms (decrease in the number of morning erections, reduced potency, and low libido) of at least moderate severity assessed using the Aging Males' Symptoms Scale. Results Decreased frequency of morning erections, reduced potency, and low libido were experienced by 56%, 62%, and 55% of men with HF, respectively; whereas 67%, 61%, and 44% of subjects complained of at least one, two, and three symptoms, respectively. Hypogonadal TT and eFT were observed in 34% and 47% of patients, respectively; and in 33% subjects, both TT and eFT were reduced. Finally, 30 men with HF (15%) were diagnosed with LOH as compared with 2% in a European male population (EMAS). In a multivariable model, older age and higher serum uric acid were independently associated with greater LOH prevalence (both P < 0.05). Among men aged ≤60 years (but not in those aged >60 years), LOH increased 5-year all-cause mortality in the univariable model; however, when adjusted for HF severity, the association lost its statistical significance. Conclusions Men with systolic HF commonly report sexual complaints. LOH—the combination of sexual dysfunction and testosterone deficiency—occurs more frequently than in a general male population. LOH does not affect long-term mortality, when adjusted for HF severity.
Use of Hyla cinerea in Assay of Melanophorotropic Potency of ACTH (1954)

In mice, a progressive lowering of blood nucleotide during pregnancy occurs simultaneously with an increase in nucleotide level for the embryos of such animals. Injection of adenylic acid into tumor-bearing mice produces a lowering of the nucleotide level in the tumor tissue, in contrast with no significant effect on the nucleotide level in embryo tissue. The liver and spleen undergo a lowering of tissue ATP as a function of pregnancy, and to a lesser extent as a function of tumor-growth. No significant alteration in muscle nucleotide is seen for mice bearing implanted tumors. The injection of adenylic acid elevates the ATP level in liver and spleen of normal, tumor-bearing, and pregnant animals, but, at least in the case of normal and tumor-bearing mice, has no effect on muscle nucleotide. Following adenylic acid injection, liver tissue from normal, tumor-bearing, and pregnant mice shows an increase in total purine, whereas the spleen does not.
Development of nondestructive crack inspection technique for conveyance roll using vibro-thermography (2012)

In recent study, active thermography has reached a high status as an easy and speedy defects inspection method in a NDT field. This paper newly proposes a non-disassembly and non-contact NDT method using the Vibro-Thermography for detecting and evaluating of fatigue cracks at neck parts of the conveyance roll in the steel making plant. In this method, fatigue cracks are detected as localized high temperature areas caused by friction and impact at crack surfaces with an infrared thermography, applying a high-amplitude ultrasonic vibration. In the case of the roll surface is covered with lubricating grease or dust, the crack detectability is shown. Self reference lock-in data processing technique is applied for improvement of signal noise ratio in the crack detection process. This technique makes it possible to perform correlating process without an external reference signal. Time and cost saving inspection method in the neck part of conveyance roll is carried out using this NDT technique.
Penicillamine should not be used as initial therapy in Wilson's disease (1999)

No abstract.
<title>PDT-induced apoptosis in brain tissue in vivo: a retrospective study</title> (1999)

The apoptotic response of normal brain and intracranial VX2 tumor following photodynamic therapy mediated by five different photodynamic drugs, Photofrin, ALA, AlClPc, SnET<SUB>2</SUB> and mTHPC, was evaluated in a preliminary retrospective analysis. Rabbit brain, with or without tumor, was treated by PDT with interstitial light delivery. Histological sections at 24 h post PDT were assessed by the TUNEL assay. Confocal fluorescence microscopy was used to determine the total apoptotic cell count and the spatial distribution of apoptotic bodies within the tissue. The data were confirmed qualitatively by light microscopy on adjacent H&E-stained sections. Light-only and drug-only controls produced background levels. The highest apoptotic count was seen with Photofrin. The counts in AlClPc-treated animals were not above the background level, while the other 3 photosensitizers gave intermediate levels. With some, but not all, drugs the spatial distribution of apoptotic bodies correlated well with the light fluence distribution. Apoptosis was seen outside the zone of frank coagulative necrosis. There was not apparent drug-dose dependency at the relatively high doses used here. The retrospective nature of this study did not allow optimization of the treatment parameters. Nevertheless, the findings have potentially significant implications, both for understanding the mechanisms of apoptosis in brain tissue and for improving the clinical use of PDT for treatment of patients with malignant brain tumors.
Frequency-encoded laser-induced fluorescence for multiplexed detection in infrared-mediated quantitative PCR (2014)

A frequency-modulated fluorescence encoding method was used as a means to increase the number of fluorophores monitored during infrared-mediated polymerase chain reaction. Laser lines at 488 nm and 561 nm were modulated at 73 and 137 Hz, respectively, exciting fluorescence from the dsDNA intercalating dye, EvaGreen, and the temperature insensitive dye, ROX. Emission was collected in a color-blind manner using a single photomultiplier tube for detection and demodulated by frequency analysis. The resulting frequency domain signal resolved the contribution from the two fluorophores as well as the background from the IR lamp. The detection method was successfully used to measure amplification of DNA samples containing 10(4)-10(7) starting copies of template producing an amplification efficiency of 96%. The utility of this methodology was further demonstrated by simultaneous amplification of two genes from human genomic DNA using different color TaqMan probes. This method of multiplexing fluorescence detection with IR-qPCR is ideally suited as it allows isolation of the signals of interest from the background in the frequency domain and is expected to further reduce the complexity of multiplexed microfluidic IR-qPCR instrumentation.
Linear Mesostructures in DNA–Nanorod Self-Assembly (2013)

The assembly of molecules and nanoscale objects into one-dimensional (1D) structures, such as fibers, tubules, and ribbons, typically results from anisotropic interactions of the constituents. Conversely, we found that a 1D structure can emerge via a very different mechanism, viz, the spontaneous symmetry breaking of underlying interparticle interactions during structure formation. For systems containing DNA-decorated nanoscale rods, this mechanism, driven by flexible DNA chains, results in the formation of 1D ladderlike mesoscale ribbons with a side-by-side rod arrangement. Detailed structural studies using electron microscopy and in situ small-angle X-ray scattering (SAXS), as well as analysis of assembly kinetics, reveal the role of collective DNA interactions in the formation of the linear structures. Moreover, the reversibility of DNA binding facilitates the development of hierarchical assemblies with time. We also observed similar linear structures of alternating rods and spheres, which implies that the discovered mechanism is generic for nanoscale objects interacting via flexible multiple linkers.
Changes in Ophthalmic Acid and Free Amino Acid in Rats Fed with an Excess Methionine Diet (1989)

Journal Article Changes in Ophthalmic Acid and Free Amino Acid in Rats Fed with an Excess Methionine Diet Get access Takanori Kasai, Takanori Kasai Department of Agricultural Chemistry, Faculty of Agriculture, Hokkaido University, Sapporo 060, Japan Search for other works by this author on: Oxford Academic Google Scholar Yuichi Otobe, Yuichi Otobe Department of Agricultural Chemistry, Faculty of Agriculture, Hokkaido University, Sapporo 060, Japan Search for other works by this author on: Oxford Academic Google Scholar Shuhachi Kiriyama Shuhachi Kiriyama Department of Agricultural Chemistry, Faculty of Agriculture, Hokkaido University, Sapporo 060, Japan Search for other works by this author on: Oxford Academic Google Scholar Agricultural and Biological Chemistry, Volume 53, Issue 2, 1 February 1989, Pages 553–556, https://doi.org/10.1080/00021369.1989.10869323 Published: 01 February 1989 Article history Received: 19 August 1988 Published: 01 February 1989