gebhart/patrepeval huggingface dataset:
Transcription Factor Networks in Drosophila melanogaster (2014)

Specific cellular fates and functions depend on differential gene expression, which occurs primarily at the transcriptional level and is controlled by complex regulatory networks of transcription factors (TFs). TFs act through combinatorial interactions with other TFs, cofactors, and chromatin-remodeling proteins. Here, we define protein-protein interactions using a coaffinity purification/mass spectrometry method and study 459 Drosophila melanogaster transcription-related factors, representing approximately half of the established catalog of TFs. We probe this network in vivo, demonstrating functional interactions for many interacting proteins, and test the predictive value of our data set. Building on these analyses, we combine regulatory network inference models with physical interactions to define an integrated network that connects combinatorial TF protein interactions to the transcriptional regulatory network of the cell. We use this integrated network as a tool to connect the functional network of genetic modifiers related to mastermind, a transcriptional cofactor of the Notch pathway.
Probabilistic Analyses: Issues and Methods (1997)

This chapter contains sections titled: Epistemic Probabilities and the Quantification of Belief Research on the Quantification of Belief Probabilistic Assessment Plan Telling Stories Using Likelihoods: Sensitivity Analyses
Electrochemical Characterization of Biotin Functionalized and Regular Single-Walled Carbon Nanotube Coatings. Application to Amperometric Glucose Biosensors (2009)

International audience
[Effect of inhalation of hair spray on the rat respiratory system]. (1986)

A study of the effects of inhaled hair-spray was performed on 118 specific pathogen free male Wistar rats (71 test animals and 47 controls). Rats were exposed for 5 min, three times a day, five days per week, during one to eight weeks, in two plexiglas chambers, one for controls and one for test animals. The chambers were 20 1 in size, but only the noses of the animals were exposed to ambiant air or to a hair-spray aerosol polluted atmosphere. In the test chamber, the hair-spray was aerosolized for 30 s at the beginning of each exposure, using about 30 g of nebulized product. This hair-spray amount was high, although non-lethal (70% of DL0, 45% of DL50 and 26% of DL100). The hair-spray exposed rats exhibited significant modifications in the antixenic defence mechanisms of their respiratory system: diminished ciliary activity of the tracheal epithelium (-26%), increased number of harvested pulmonary macrophages (+52%), but decreased phagocytic ability of these cells (-8%). However we did not observe histologic differences in the respiratory system of exposed and control rats.
REMBLAYAGE DES TRANCHEES ET REFECTION DES CHAUSSEES - ANNEXE 4 LISTE DES MATERIELS (1999)

Ce guide definit les regles techniques et propose des demarches permettant aux intervenants, qu'ils soient gestionnnaires de voiries, entrepreneurs, concessionnaires, permissionnaires ou occupant de droit, de maitriser la realisation du remblayage et de la refection, du projet jusqu'a la realisation des travaux. (voir no dirr 131094). L'annexe 4, contenant la liste des materiels est mis a jour en mai 1999, remplace l'annexe de janvier 1994.
Angiotensin II AT1 receptor gene polymorphism and microalbuminuria in essential hypertension (2001)

The objective of this study was to analyze the relationship of polymorphisms of the angiotensin II AT1 receptor gene with microalbuminuria in a group of young adults with essential hypertension. Essential hypertensives, less than 50 years old, never previously treated with antihypertensive drugs, and in absence of diabetes mellitus were included. Office blood pressure (BP), 24-h ambulatory BP monitoring, urinary albumin excretion (UAE) measurements, and DNA analysis were performed. Polymorphisms of the angiotensin II AT1-receptor gene (A1166C and C573T) were studied by polymerase chain reaction and single-strand conformation polymorphism techniques. One hundred eighty-three patients, 49 (27%) microalbuminurics, were included. Office and ambulatory BP values were significantly higher in the microalbuminuria group. No differences in the presence of microalbuminuria were observed among the genotypes of either A1166C or C573T polymorphisms of the angiotensin II receptor AT1 gene, or in the allele frequency of the A1166C or the C573T polymorphism. LogUAE was significantly different among genotypes of the C573T polymorphism [CC 1.30(1.15-1.45), CT 1.14(1.00-1.28), and TT 0.94(0.68-1.20), P < .05]. Both office and ambulatory blood pressure and the TT/C573T genotype were independently related to logUAE, and, at the same BP values, UAE was lower in subjects with this genotype. We have found that the C573T polymorphism is on linkage disequilibrium with A1166C, as the 573T allele is closely linked to the presence of the 1166A allele, but not vice versa. Haplotype analysis among subjects with the AA genotype for the A1166C polymorphism confirms the influence of the TT genotype of the C573T polymorphism on the UAE in hypertensives. The C573T polymorphism of the angiotensin II receptor AT1 gene seems to be a genetic protective factor for UAE in a population of essential hypertensives.
Strain-modulated epitaxy: A flexible approach to 3-D band structure engineering without surface patterning (1996)

Thin compliant growth substrates have been used to reduce the strain in lattice-mismatched overlayers during epitaxial growth. This letter reports a new thin compliant substrate technology which allows these thin substrates to be patterned on the bottom, bonded surface. This lateral strain variation (inverted stressor) in the growing film can be combined with the additional effects of strain-dependent growth kinetics to realize the lateral control of composition and thickness without any surface topography on the substrate. Initial demonstrations of the growth of InGaAs on GaAs bottom-patterned thin substrates are presented herein.
The major peripheral myelin protein zero gene: structure and localization in the cluster of <i>Fcγ</i> receptor genes on human chromosome 1q21.3 – q23 (1993)

We have characterized the human gene encoding the major peripheral myelin protein zero (P0) and assigned it, by in situ hybridization, to the q21.3-q23 region of human chromosome 1. This region is known to contain a cluster of interspersed genes coding for the related human leukocyte receptors of the Fc portion of the immunoglobulin G (Fc gamma RI, II, III). This colocalization was refined by the finding of a yeast artificial chromosome (YAC) of the Centre d'Etude du Polymorphisme Humain (CEPH) library, hybridizing to the P0 and Fc gamma RIIA genes, demonstrating their physical linkage. These data may have important implications in demyelinating diseases studies like Charcot-Marie-Tooth disease type 1B (CMT1B).
Robust Biomechanical Model-Based 3-D Indoor Localization and Tracking Method Using UWB and IMU (2016)

This paper proposes a robust sensor fusion algorithm to accurately track the spatial location and motion of a human under various dynamic activities, such as walking, running, and jumping. The position accuracy of the indoor wireless positioning systems frequently suffers from non-line-of-sight and multipath effects, resulting in heavy-tailed outliers and signal outages. We address this problem by integrating the estimates from an ultra-wideband (UWB) system and inertial measurement units, but also taking advantage of the estimated velocity and height obtained from an aiding lower body biomechanical model. The proposed method is a cascaded Kalman filter-based algorithm where the orientation filter is cascaded with the robust position/velocity filter. The outliers are detected for individual measurements using the normalized innovation squared, where the measurement noise covariance is softly scaled to reduce its weight. The positioning accuracy is further improved with the Rauch-Tung-Striebel smoother. The proposed algorithm was validated against an optical motion tracking system for both slow (walking) and dynamic (running and jumping) activities performed in laboratory experiments. The results show that the proposed algorithm can maintain high accuracy for tracking the location of a subject in the presence of the outliers and UWB signal outages with a combined 3-D positioning error of less than 13 cm.
Au(111) characterization and Si(100)H surface imaging with the UHV-LT 4-STM (2017)

International audience
Fractures of the Proximal Third of the Tibial Shaft Treated With Intramedullary Nails and Blocking Screws (2001)

Blocking screws are effective to help obtain and maintain alignment of fractures of the proximal third of the tibial shaft treated with intramedullary nails.
BUILDING EYECARE SERVICES IN A DEVELOPING NATION - THE EAST TIMOR EYE PROGRAM (ETEP). (2001)

Monday, December 10, 2001. Posters: Academy Information, Public Health, Ocular Disease, Low Vision, Cornea and Contact Lenses: PDF Only
Triplet Properties and Photoinduced Electron-Transfer Reactions of C<sub>120</sub>, the [2+2] Dimer of Fullerene C<sub>60</sub> (1999)

Photophysical and photochemical properties of a [2+2] fullerene dimer, C120, have been characterized by laser flash photolysis. By laser irradiation to C120, a triplet−triplet absorption band appeared at 700 nm. From triplet energy-transfer reactions with some energy acceptors, photophysical properties of C120, including triplet energy, extinction coefficient, and quantum yield of the intersystem crossing process, were estimated. Reduction of C120 in the ground state by tetrakis(dimethylamino)ethylene resulted in decomposition into C60•- and C60. Photoinduced electron transfer from N,N,N‘,N‘-tetramethyl-1,4-phenylenediamine to triplet-excited C120 was confirmed by the observation of a transient absorption band due to the radical anion of C120 at 1020 nm. Electron-transfer rates between triplet-excited C120 and various electron-donors were explained by the semiempirical Rehm−Weller relation. Oxidation of triplet-excited C120 was also observed by employing tetracyanoethylene as an electron-acceptor.
Dielectric breakdown in high-ε films for ULSI DRAMs (1993)

Abstract Dielectric breakdown has been studied in several materials intended for high-dielectric DRAMs (dynamic random access memories), emphasizing lead zirconate-titanate (PZT) and barium strontium titanate (BST) ceramics. In this paper we present our results on PZT. A second paper will deal with BST. In order to distinguish among impulse thermal breakdown, de thermal breakdown, and avalanche breakdown mechanisms, studies have been carried out as functions of temperature, electrode material and shape, frequency and duration of applied fields, and specimen size and shape. Notable in the results is the fact that maximum breakdown field varies directly with electrode work function but is uncorrelated with electrode thermal conductivity; this militates against a purely thermal breakdown interpretation and instead favors an avalanche mechanism in which the iniation step is impact ionization of Ti ions from electrons emitted from the electrodes, followed by thermal run-away. Thickness dependence also favors avalanche mechanisms, but dependence upon applied field ramp rate favors an impulse thermal mechanism. Taken altogether, the data indicate characteristics of mixed impulse-thermal and avalanche mechanisms: i.e., electron-initiation followed by thermal run-away. Key Words: high dielectricPZTlead zirconate-titanatebreakdown
Granulocyte activation via a binding site near the C-terminal region of complement receptor type 3 <i>alpha</i>-chain (CD11b) potentially involved in intramembrane complex formation with glycosylphosphatidylinositol-anchored Fc gamma RIIIB (CD16) molecules. (1995)

We report that engagement of a particular epitope near the C-terminal region of complement receptor type 3 (CR3, CD11b/CD18) alpha-chain with CD11b mAb VIM12 induces granulocyte activation with a rise in cytosolic-free Ca2+, actin polymerization, an up-regulation of CR3 cell surface expression and enhanced adhesiveness. Induction of enhanced adhesiveness and homotypic aggregation of human granulocytes represents an active process. It is temperature and energy dependent, requires divalent cations, and an intact cytoskeleton. The mAb VIM12-induced enhanced adhesiveness seems to be mediated, at least in part, by activated CR3 molecules. It can be significantly inhibited, although not completely abolished, with blocking mAbs against adhesiotopes of CR3. VIM12-induced adhesion could be blocked with the serine/threonine inhibitors okadaic acid and calyculin A and with dibuturyl-cAMP but not with the protein kinase inhibitors herbimycin A and staurosporine. We further present evidence that the particular molecular region of CR3 recognized by mAb VIM12 might be involved in the reported intramembrane sugar-lectin type interaction and complex formation between transmembrane CR3 and glycosylphosphatidylinositol (GPI)-anchored Fc gamma RIIIB (CD16) molecules on human granulocytes. Binding of mAb VIM12 to CR3 on granulocytes enhances the release of GPI-anchored Fc gamma RIIIB molecules from granulocytes upon phosphoinositol-phospholipase C treatment. The sugar preparation N-acetyl-D-glucosamine, previously shown to dissociate CR3-Fc gamma RIIIB complex formation, inhibits mAb VIM12 binding. Engagement of CR3 with mAb VIM12 may thus mimic a biologically relevant intramembrane cooperation between two distinct receptor molecules on human granulocytes.
Narrowly separated<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" display="inline"><mml:mi>M</mml:mi><mml:mo>=</mml:mo><mml:mn>0</mml:mn><mml:mn/></mml:math>and<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" display="inline"><mml:mi>S</mml:mi><mml:mo>=</mml:mo><mml:mn>0</mml:mn><mml:mn/></mml:math>compensation points in epitaxial films of the<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" display="inline"><mml:mrow><mml:msub><mml:mrow><mml:mi mathvariant="normal">Eu</mml:mi></mml:mrow><mml:mrow><mml:mi>z</mml:mi></mml:mrow></mml:msub></mml:mrow><mml:mrow><mml:msub><mml:mrow><mml:mi mathvariant="normal">Y</mml:mi></mml:mrow><mml:mrow><mml:mn>3</mml:mn><mml:mo>−</mml:mo><mml:mi>z</mml:mi></mml:mrow></mml:msub></mml:mrow><mml:mrow><mml:msub><mml:mrow><mml:mi mathvariant="normal">Ga</mml:mi></mml:mrow><mml:mrow><mml:mi>t</mml:mi></mml:mrow></mml:msub></mml:mrow><mml:mrow><mml:msub><mml:mrow><mml:mi mathvariant="normal">Fe</mml:mi></mml:mrow><mml:mrow><mml:mn>5</mml:mn><mml:mo>−</mml:mo><mml:mi>t</mml:mi></mml:mrow></mml:msub></mml:mrow><mml:mrow><mml:msub><mml:mrow><mml:mi mathvariant="normal">O</mml:mi></mml:mrow><mml:mrow><mml:mn>12</mml:mn></mml:mrow></mml:msub></mml:mrow></mml:math>system (1980)

A series of 18 epitaxial films having the formula ${\mathrm{Eu}}_{z}{\mathrm{Y}}_{3\ensuremath{-}z}{\mathrm{Ga}}_{t}{\mathrm{Fe}}_{5\ensuremath{-}t}{\mathrm{O}}_{12}$ were grown by stepwise modifications of an initial yttrium iron garnet (YIG) melt, to the composition ${\mathrm{Y}}_{2}$${\mathrm{Eu}}_{1}$${\mathrm{Ga}}_{1.8}$${\mathrm{Fe}}_{3.2}$${\mathrm{O}}_{12}$. The relation between $z$ and $t$ was set to be $z=0.492t+0.172$ in order to maintain the lattice-parameter match between the ${\mathrm{Gd}}_{3}$${\mathrm{Ga}}_{5}$${\mathrm{O}}_{12}$ substrate and the films throughout the series. Relevant material parameters have been measured by microwave techniques and derived from earlier data: the effective gyromagnetic ratio ${g}_{\mathrm{eff}}$, the uniaxial anisotropy ${H}_{u}$, and Gilbert's damping parameter $\ensuremath{\alpha}$. It is clearly shown by the data that ${g}_{\mathrm{eff}}$ and $\ensuremath{\alpha}$ diverge at the angular momentum compensation point, while ${H}_{u}$ diverges at the magnetization compensation point. The separation between the angular momentum compensation point ${t}_{S}$ and the magnetization compensation point ${t}_{M}$ was found to be ${t}_{S}\ensuremath{-}{t}_{M}\ensuremath{\approx}0.06$ in accord with the theory for low $c$-site europium occupancy as compared with ${t}_{S}\ensuremath{-}{t}_{M}=0.45$ for $c$ sites entirely filled with europium.
Synthesis of chiral atropoisomeric square-planar nickel(II) and copper(II) complexes formed by macrocyclic ligands containing pendant polyether groups and a quaternary ammonium group (1990)

Novel copper(II) and nickel(II) complexes of macrocyclic Schiff bases formed from derivatives of N,N′-bis(2-benzoylphenyl)oxamide and 1,2-diaminoethane have been prepared. In one case, separation of atropoisomeric chiral complexes has been achieved by preparative chromatography on SiO2. Quaternization of some of the complexes with an excess of MeI affords chiral complexes possessing a positively charged quaternary ammonium group and polyether podands in addition to a weakly electrophilic metal site.
Laser alignment system for helical pinhole SPECT (2003)

Pinhole SPECT is very sensitive to mechanical alignment because it is a magnifying geometry. Because of magnification, small mechanical shifts of the aperture result in a large change in the projection data. Helical orbits may be used for pinhole SPECT to improve the completeness of the projection data. This has been done by using a research scanner to provide rotation while a linear stage produces axial movement. The combined effect is a helical orbit. However, reconstruction quality may be limited by the alignment of the linear stage with the axis of rotation of the scanner. We have developed a laser system for aligning the linear stage. We present the system design and estimate the accuracy of its alignment Experimental helical pinhole SPECT data are presented.
Phosphinothricin Acetyltransferases Identified Using <i>In Vivo</i> , <i>In Vitro</i> , and Bioinformatic Analyses (2016)

Acetylation of small molecules is widespread in nature, and in some cases, cells use this process to detoxify harmful chemicals. Streptomyces species utilize a Gcn5 N-acetyltransferase (GNAT), known as Bar, to acetylate and detoxify a self-produced toxin, phosphinothricin (PPT), a glutamate analogue. Bar homologues, such as MddA from Salmonella enterica, acetylate methionine analogues such as methionine sulfoximine (MSX) and methionine sulfone (MSO), but not PPT, even though Bar homologues are annotated as PPT acetyltransferases. S. enterica was used as a heterologous host to determine whether or not putative PPT acetyltransferases from various sources could acetylate PPT, MSX, and MSO. In vitro and in vivo analyses identified substrates acetylated by putative PPT acetyltransferases from Deinococcus radiodurans (DR_1057 and DR_1182) and Geobacillus kaustophilus (GK0593 and GK2920). In vivo, synthesis of DR_1182, GK0593, and GK2920 blocked the inhibitory effects of PPT, MSX, and MSO. In contrast, DR_1057 did not detoxify any of the above substrates. Results of in vitro studies were consistent with the in vivo results. In addition, phylogenetic analyses were used to predict the functionality of annotated PPT acetyltransferases in Burkholderia xenovorans, Bacillus subtilis, Staphylococcus aureus, Acinetobacter baylyi, and Escherichia coli IMPORTANCE: The work reported here provides an example of the use of a heterologous system for the identification of enzyme function. Many members of this superfamily of proteins do not have a known function, or it has been annotated solely on the basis of sequence homology to previously characterized enzymes. The critical role of Gcn5 N-acetyltransferases (GNATs) in the modulation of central metabolic processes, and in controlling metabolic stress, necessitates approaches that can reveal their physiological role. The combination of in vivo, in vitro, and bioinformatics approaches reported here identified GNATs that can acetylate and detoxify phosphinothricin.
Effect of magnesium ion on the structure of the 5S RNA from Escherichia coli. An imino proton magnetic resonance study of the helix I, IV, and V regions of the molecule (1986)

The imino proton nuclear magnetic resonance spectrum of Escherichia coli 5S ribonucleic acid (RNA) changes when the Mg2+ ion concentration drops below physiological levels. The transition between the physiological and low magnesium spectral forms of 5S RNA has a midpoint at approximately 0.3 mM Mg2+. Many of the most conspicuous changes observed in the downfield spectrum of 5S RNA as the magnesium concentration is reduced are due to adjustments in the structures of helices I and IV and the disappearance of resonances originating in helix V. The binding of ribosomal protein L25 to 5S RNA in the absence of magnesium stabilizes helix V structures.
Effect-specific analysis of propagation parameters (1992)

Results of propagation measurements with the satellite OLYMPUS carried out at 12.5, 20, and 30 GHz at the Research Center of the Deutsche Bundespost Telekom are discussed. In particular, attenuation, scintillation, and depolarization measurements are analyzed with special emphasis on frequency scaling of the various effects.
Co-Expression of DevR and DevRN-Aph Proteins Is Associated with Hypoxic Adaptation Defect and Virulence Attenuation of Mycobacterium tuberculosis (2010)

The hypoxic defect of Comp1 is associated to a defect in DevR expression level. The demonstrated repression of DevR function by DevR(N)-Kan suggests that such a knockdown approach could be useful for evaluating the activity of DevRS and other two-component signaling pathways. Further investigation is necessary to elucidate the mechanism underlying Comp1 attenuation.
Mangoes in Krian. (1940)

To clarify the problem in the measurement of renal plasma flow and glomerular filtration rates in diabetes, the effect of glucose on the determination of para-aminohippuric acid (PAH) and inulin was examined. The concentration of urinary PAH in glucosuric diabetic subjects decreased after the storage of urine samples because of the glycation of PAH. Therefore, glucose must be removed by the acid treatment before the determination of the concentrations of urinary PAH. Since glucose can interfere with the assay of inulin, the sample must be treated with NaOH prior to the determination of the inulin concentration. GFR of the subjects with type 2 diabetes was next examined. GFR in the subjects with a duration of diabetes less than 10 years was significantly higher than that in the subjects with a duration of diabetes more than 10 years. Thus, the subjects with short-term type 2 diabetes may present with hyperfiltration similar to the subjects with short-term type 1 diabetes.