gebhart/patrepeval huggingface dataset:
Age‐related disease in recurrent outbreaks of phocid herpesvirus type‐I infections in a seal rehabilitation centre: evaluation of diagnostic methods (1997)

The prevalence and clinical signs of phocid herpesvirus type-1 (PhHV-1) infections among harbour seals (Phoca vitulina) in a seal rehabilitation centre in the Netherlands were monitored between June and September 1993 and 1994 when 34 and 36 seals, respectively, were rehabilitated. In both years PhHV-1-related disease outbreaks occurred in the pupping season. PhHV-1 infections were diagnosed by the demonstration of a more than four-fold increase in virus neutralising serum antibodies in paired serum samples, by the isolation of the virus from swab samples in primary seal kidney cells, and by the detection of PhHV-1 DNA with a polymerase chain reaction (PCR) assay in swab samples. This assay targets a 290 bp fragment of the glycoprotein D (gD) gene equivalent of PhHV-1. The PCR assay when combined with Southern blotting (PCR-SB) was approximately 1000 times more sensitive than virus isolation when tested with serially diluted samples from PhHV-1-infected cell cultures. In contrast with virus isolation, the PCR-SB scored as positive all the animals with serological evidence of PhHV-1 infection. The majority of seals present in the centre during the outbreaks contracted the infection and developed benign upper respiratory disease. However, the severity of PhHV-1-related disease was inversely correlated with age and fatal generalised infections occurred only in neonates.
Antibody Responses to HPV6b E Polyproteins and Production of Monoclonal Antibodies (2002)

A range of fusion constructs (expressed in Escherichia coli) were produced that contained two or more HPV6b E proteins, producing a single continuous amino acid sequence corresponding to the sequences of the individual E proteins. The constructs also included a C-terminal hexahistidine tag fused in-frame to aid purification. The fusion proteins (polyproteins) were semipurified by Ni(++) metal affinity chromatography under denaturing conditions. Immunization of BALB/c mice with these polyproteins resulted in the production of specific E protein antibodies. The draining lymph nodes from these mice were used to produce monoclonal antibodies (MAbs). The specificity of the polyclonal and MAbs was confirmed by immunoblotting and by screening for reaction with a series of synthetic peptides of E proteins. HPV E polyproteins were found to be immunogenic and immunization with the polyproteins resulted in specific antibody responses to the component E proteins.
The energy and entropy of solution of silver chloride in methanol-water mixtures. Part I (1945)

H. N. Parton, D. J. Davis, F. Hurst and G. D. Gemmell, Trans. Faraday Soc., 1945, 41, 575 DOI: 10.1039/TF9454100575
Thermal emission and capture rates of holes at the gold donor level in silicon (1987)

Thermal emission rate and capture cross section of holes at the gold donor level are measured in silicon n+/p junctions with boron acceptor concentrations of 1.5×1012 cm−3 to 2.8×1015 cm−3, showing no boron concentration dependencies. The emission rate is independent of electric field in the range of 102 to 2×104 V/cm. However, the emission rate varies with the voltage applied to the insulated-gate guard ring, requiring re-evaluation of previously published data. The thermal capture cross section agrees with most of the published work. The magnitude of the capture cross section and its temperature independence support a neutral potential model for hole capture at the gold donor level.
Factors Influencing Utilization of the Primary Prevention Implantable Defibrillator (2015)

In a contemporary urban tertiary care setting, age, sex and heart failure symptom class were associated with ICD utilization, with socioeconomic/insurance status also potentially playing a role. These findings have implications for optimizing appropriate utilization of the prophylactic ICD and warrant follow-up in larger, more diverse populations.
Thermo–fluid mechanisms controlling droplet based materials processes (2002)

AbstractAbstractRecently, the successful application of thermal spray deposition techniques to the manufacture of bulk materials and thin film coatings with improved structural properties has illuminated the need for a fundamental understanding of the physical phenomena that control the deposition process. However, the spray parameters have a complex statistical interdependence that renders their analysis difficult. Discrete droplet deposition processes provide phenomenologically simpler systems than thermal spray deposition processes while still retaining their salient features. Furthermore, discrete droplet deposition processes have found important manufacturing applications from soldering to rapid prototyping. Consequently, this review focuses on recent research in the area of droplet based materials processes. The emphasis is on fluid dynamics and thermal aspects as they relate to materials science issues. The design and operation of a number of representative droplet deposition approaches is also discussed in detail, paying special attention to applications in near net shape manufacturing and digital microfabrication. Finally, outstanding questions are identified.
Überwachung von Betonkonstruktionen mit eingebetteten Ultraschallsensoren (2014)

Abstract Die immer komplexere Konstruktionsweise von Neubauten einerseits und die alternde Infrastruktur andererseits erfordern in manchen Fällen eine dauerhafte Überwachung, um besondere Einwirkungen und gegebenenfalls Schädigungen rechtzeitig und genau zu erkennen. Hierfür ist eine Vielzahl von Methoden und Sensoren verfügbar. Das Portfolio weist aber insbesondere bei der Detektion langsamer, räumlich begrenzter Veränderungen Lücken auf. Hierfür sind bisher sehr aufwändige Untersuchungen oder Installationen notwendig. In der vorliegenden Arbeit werden Sensoren und zugehörige Auswertemethoden für die Ultraschalltransmission vorgestellt, die sich für die zerstörungsfreie, dauerhafte Überwachung von Beton eignen. Direkt oder nachträglich in Betonkonstruktionen eingebaut, ermöglichen sie eine nicht nur lokale, sondern größere Raumbereiche umfassende Dauerüberwachung von Änderungen der Materialeigenschaften. Das Prinzip der Ultraschalltransmission und die verschiedenen Einflussparameter werden vorgestellt. Zu letzteren gehören neben der Belastung und Schädigung auch Umweltparameter wie Temperatur und Feuchte. Verschiedene Methoden zur Datenanalyse, wie z. B. die Codawelleninterferometrie, ermöglichen eine Detektion kleinster Veränderungen. Die in den Beton einzubettenden Ultraschallsensoren werden vorgestellt und ihr Einbau und Betrieb beschrieben. Als Beispiele für Anwendungen werden Frost‐Tauwechsel‐Experimente im Labor, die Detektion von lokalen Lasten im Technikumsmaßstab und der Einsatz an realen Brücken diskutiert. Die Sensoren sind zum Teil bereits seit mehreren Jahren in Probeobjekte eingebettet und liefern zuverlässig wertvolle Daten. Monitoring of concrete constructions by embedded ultrasonic sensors Challenging new constructions and the ageing infrastructure are increasing the demand for permanent monitoring of loads and damages. Various methods and sensors are used for this purpose. But the technologies available today have difficulties in detecting slowly progressing locally confined damages. Extensive investigations or instrumentations are required so far for this purpose. In this study we present new sensors and data processing methods for ultrasonic transmission, which can be used for non‐destructive permanent monitoring of concrete. They can be mounted during construction or thereafter. Larger volumes can be monitored by a limited number of sensors for changes of material properties. The principles of ultrasonic transmission and influencing factors are presented. This latter include load, damages as well as environmental parameters as temperature or moisture. Various methods for data processing, e. g. coda wave interferometry are introduced. They allow the detection of very small changes in the medium. The embedded sensors are shown including mounting and operation. Application examples so far include small scale laboratory freeze‐thaw experiments, localizing loads in larger concrete models and monitoring load effects on real structures. Some sensors are operating already for several years.
Transcriptional control of metabolic regulation genes by carbohydrates (1994)

Glucose can modulate the transcription of many genes, particularly those encoding enzymes of liver metabolism. The transcriptional effect of glucose can be indirect, being mediated in vivo by hormonal variations, especially increase in insulin and decrease in glucagon secretion. Whereas the transcription of the glucokinase gene, for example, is stimulated by insulin without the aid of glucose, the transcriptional activation of most glycolytic and lipogenic genes in hepatocytes requires the presence of both glucose and insulin. The role of insulin in the activation of these genes seems mainly to stimulate glucokinase synthesis, and thus to permit glucose phosphorylation. In some cells in which hexokinase activity is constitutive, the glucose-dependent activation of the same genes does not require insulin and, in addition, can be produced by the nonmetabolisable analog, 2-deoxyglucose. In hepatocytes, the insulin effect on the glucose-dependent activation of the L-pyruvate kinase gene can be reproduced by fructose at low concentrations. Fructose probably acts through the fructose 1-phosphate dependent deinhibition of glucokinase activity. A glucose/carbohydrate element has been identified on the L-type pyruvate kinase and spot 14 gene promoters. It is able to bind, in vitro, transcriptional factors of the MLTF/USF family and could act in cooperation with tissue-specific contiguous elements, such as the HNF4 binding site in the L-type pyruvate kinase gene.
[Estimation of nitrogen concentration in cotton leaf based on canopy reflectance spectra]. (2007)

Through analyzing the relationships of nitrogen concentration in cotton leaf under different nitrogen supply levels with canopy multi-spectral reflectance and its derived ratio vegetation index (RVI, rholambda1/rholambda2), normalized difference vegetation index (NDVI, (rho(lambda1) - rho(lambda2))/(rho(lambda1)) + rho(lambda2)) and differential vegetation index (DVI, rho(lambda1) - rho(lambda2)), the sensitive wave bands and prediction functions of cotton leaf nitrogen concentration were worked out. The vegetation index composed of visible region (610, 660, 680 and 710 nm) and near infrared region (760, 810, 870, 950, 1 100 and 1 220 nm) had a higher correlation with the nitrogen concentration in cotton leaf, and the RVI composed of 950 nm and 710 nm could best predict the leaf nitrogen concentration. The validation with independent field experimental data indicated that RVI (950 nm and 710 nm) -based model was suitable for estimation of leaf nitrogen concentration of different cotton cultivars at their different growth stages.
Surface-Coupled Microsphere Resonators (2014)

A novel micro-optical amplifier is evaluated experimentally and theoretically. Comprising a microsphere (onto which a gain medium is tethered) and a plasmonic surface, this micro-optical system is capable of amplifying Raman emission generated internally to the microsphere.
Protein Sparing Therapy during Periods of Starvation with Sepsis or Trauma (1973)

BLACKBURN, C. L. M.D.; FLATT, J. P. PhD.; CLOWES, G. H. A. JR. M.D.; O'DONNELL, T. F. M.D.; HENSLE, T. E. M.D. Author Information
COIL-related activities in the Czech Republic (1996)

This presentation is an historical overview of the activities related to the Chemical Oxygen- Iodine Laser conducted in the Czech Republic. It includes small scale basic research, both the experimental and the theoretical, with a view to particular topics of this laser system in the cw and the pulsed regime of generation.
Port-Access ? Coronary Artery Bypass Grafting: Technical Considerations and Results (1998)

These results demonstrate that Port-Access coronary artery bypass grafting using endovascular techniques for cardiopulmonary bypass and cardioplegic arrest can be performed safely with minimal morbidity and mortality. This technique allows multivessel revascularization on a protected, arrested heart with excellent anastomotic precision and reproducible early graft patency. Expanded use of Port-Access techniques is indicated in patients with multivessel coronary artery disease and the technique should be considered for patients with left anterior descending artery restenosis and patients with complex left anterior descending artery lesions where angioplasty results are suboptimal.
Performance of Time Warp Protocols for Transaction Management in Object Oriented Systems. (1994)

It has been pointed out (e.g., by Richard Fujimoto) that protocols developed for the difficult problem of parallel and distributed simulation can also be adapted to other distributed applications. Indeed, in the mid 1980&apos;s David Jefferson adapted his Time Warp protocol to the problem of database concurrency control. In the present work, we consider our own variants of the Time Warp for database concurrency control, and perform a simulation study to compare their performance with other more traditional protocols. The performance results obtained for the Time Warp protocol are very encouraging. The initial motivation for developing and analyzing these variants of Time Warp was to find a high performance protocol suitable for a Query Driven Simulation system. Such a system will need to enforce the consistency of concurrent/parallel operations from both a database viewpoint and a simulation viewpoint. Keywords: Database Protocols, Transaction Management, Concurrency Control, Recovery, Para...
Pichia pastoris에서 발현된 보리 알파아밀라제 Chimera 효소들의 특성 (2010)

Two different α-amylase isozymes (AMY1 and AMY2) found in barley malt share up to 80% of amino acid sequence identity with each other, but their enzymatic properties differ remarkably. AMY1 shows the highest activity at low concentration of calcium ion, while AMY2 is highly active at high calcium concentration. Meanwhile, BASI (Barley α-Amylase/Subtilisin Inhibitor) protein specifically inhibits only AMY2. In the present study, three separate regions in AMY genes (Ⅰ, Ⅱ, and Ⅲ) were assigned on the basis of restriction enzyme sites and four kinds of chimeric amylases have been obtained by swapping a part of regions with each other. Each chimera gene was successfully over-expressed in Pichia pastoris. From the results of enzymatic characterization, both AMY211 and AMY122 showed the mixed or intermediate type of calcium-dependent activity between AMY1 and 2. Meanwhile, only AMY221 chimera could be significantly inhibited by BASI protein. As a result, it can be proposed that some amino acid residues in the region Ⅰ and Ⅱ, except region Ⅲ, of barley α-amylases play very important roles in calcium-dependency and interaction with BASI.
A Recurrent Problem (2011)

A 50-year-old woman from Texas had nasal stuffiness, postnasal drip, myalgias, cough, and hoarseness in early winter. Six weeks later, her nonproductive cough and hoarseness persisted. She had no fever, chills, night sweats, pain, weight loss, nausea, vomiting, or rash.
Improving Biocompatibily of Laser Micromachined Silicon Wafer by Surface Coating With Poly(Ethylene Glycol) Diacrylate and Diamond-Like Carbon for Biomedical Devices (2010)

This paper presents a study of laser micromachining and the effect of the micromachined regions on the biocompatibility in the protein adsorption for medical devices applications. From the study, the surface roughness on the micromachined area promoted the protein adsorption so it probably causes the thrombus formation for the application in the cardiovascular or blood related devices. In contrast, this incident can enhance the cell promotion that is helpful for the biomedical implant MEMS devices. To modify the surface property in biocompatibility, both Diamond-like carbon (DLC) coating and Poly ethylene glycol diacrylate (PEGDA), the protein resistant and biocompatible materials, were proposed in this research. The UV lithography for coating PEGDA and Pulse laser deposition for coating DLC on the laser micromachined substrates were applied while the coated substrates were biocompatibility tested with the Bovine Serum Albumin (BSA) to observe the protein adsorption. To image the quantity of adsorption, the fluorescein labeled BSA was used for fluorescent microscope to clearly image the reflective light. This study proposed a good opportunity for major novel techniques such as laser micromachining, PLD and UV lithography in developing and prototyping biocompatible MEMS devices.
The Role of Monomer in Alkylation Reactions of the Lithium Enolate of <i>p</i>-Phenylisobutyrophenone in Tetrahydrofuran<sup>1</sup> (1998)

The lithium salt of p-phenylisobutyrophenone (LiPhIBP) exists in tetrahydrofuran (THF) as a mixture of monomer and tetramer contact ion pairs (CIP). The equilibrium constant, K1,4 = 5.0 × 108 M-3, indicates that the lithium enolate is primarily tetrameric at higher concentrations, but the monomer is still present in significant amounts even at concentrations typical of synthesis reactions. Alkylation reactions of LiPhIBP with various alkylating agents were investigated in THF at 25 °C at concentrations of 10-3 to 10-2 M by using UV−vis spectroscopy. The kinetics followed rate laws of 0.50 to 0.30 order in the formal lithium enolate concentration but is first order in the monomer concentration. These rate studies provide direct evidence that the reactive species is the monomer, even when tetramer dominates the equilibrium.
Human Embryonic Kidney Cells: Stable Transformation with an Origin-Defective Simian Virus 40 DNA and Use as Hosts for Human Papovavirus Replication (1984)

An origin-defective mutant DNA of simian virus 40 immortalized human embryonic kidney cells, maintaining a T protein which could function for human papovavirus BK DNA replication but not for human papovavirus JC DNA replication. Neither BK virions nor capsid proteins were produced in these cells. This may indicate that the simian virus 40 T protein in human embryonic kidney cells is competent for maintaining transformation and initiating and completing DNA replication for BK but is not competent for switching to late gene functions. Furthermore, it appears that the JC DNA replication origin cannot efficiently use the simian virus 40 T protein for its DNA synthesis, as suggested by its DNA sequence data (R. Frisque, J. Virol. 46:170-176, 1983; T. Miyamura, H. Jikoya, E. Soeda, and K. Yoshiike, J. Virol. 45:73-79, 1983).
Floral Pigmentation and Expression of Anthocyanin-Related Genes in Bicolored Roses ‘Pinky Girl’ as Affected by Temporal Heat Stress (2015)

본 연구는 일시적인 고온 스트레스 처리가 복색 장미 'Pinky Girl'의 화아 착색과 안토시아닌 생합성 관련 유전자들의 발현 양상에 미치는 영향을 알아보고자 수행되었다. 꽃잎에서 cyanin의 축적은 화아의 발육단계와 연관이 있으며 화아 발육 과정에서 꽃잎이 출현하는 4단계(<TEX>$S_4$</TEX>)에서 급격하게 일어났다. 따라서 <TEX>$S_4$</TEX> 단계가 꽃잎 착색에 가장 민감한 시기로 판단된다. <TEX>$S_4$</TEX> 단계에서 3일간 고온 스트레스(<TEX>$39/18^{\circ}C$</TEX>)를 받은 고온처리구에서 개화 당시 꽃잎의 cyanin 착색이 대조구와 비교하여 45.5% 감소하였다. 한편, 안토시아닌 생합성 관련 유전자인 CHS, CHI, F3'H, DFR, ANS, 3GT, 5GT의 발현은 고온처리구에서 오히려 촉진되었다. 예외적으로 F3H의 발현은 고온처리구에서 26.7% 감소하여 'Pinky Girl'의 복색 발현에 의미 있는 유전자로 확인되었다. 하지만 대부분 안토시아닌 생합성 관련 유전자들의 발현은 꽃잎에서의 cyanin 착색 경향과 비례적이지 않았다. 따라서 장미 꽃잎의 화색소 축적은 번역 이후 효과와 조절유전자와 같은 안토시아닌 생합성과 관련된 다른 복잡한 메커니즘이 연관되어 있을 것으로 판단되었다. This study was conducted to investigate petal pigmentation and the expression patterns of anthocyanin-related genes in bicolored roses 'Pinky Girl' treated with temporal heat stress (THS). Cyanin accumulation in petals was correlated with floral bud development and started rapidly as floral buds began to open, defined as the <TEX>$4^{th}$</TEX> stage of floral bud development (<TEX>$S_4$</TEX>). This stage seems to be most susceptible to petal pigmentation. The total of cyanin pigmentation at blooming was significantly decreased (by 45.5%) with exposure to THS (<TEX>$39/18^{\circ}C$</TEX> for three days at <TEX>$S_4$</TEX>) in comparison with control. Meanwhile, the expression of anthocyanin-related genes such as CHS, CHI, F3'H, DFR, ANS, 3GT, and 5GT was relatively promoted by THS. Only F3H was less expressed (by 26.7%) with THS treatment; thus, F3H could be a key gene for bicolor promotion in 'Pinky Girl' among anthocyanin-related genes. Overall, the expression pattern of the most anthocyanin-related genes did not match the trends of cyanin pigmentation in petals. These results suggest that floral pigmentation could be associated with other mechanisms related to anthocyanin biosynthesis such as post-translational effects and regulatory genes.
Crystallographic Studies of Chicken Triose Phosphate Isomerase (1972)

One of the simplest reactions in the glycolytic pathway is the interconversion of dihydroxy acetone phosphate (DHAP) and d-glyceraldehyde-3-phosphate (GAP) which is catalyzed by triose phosphate isomerase (E.C. 5.3.1.1), TIM. The equilibrium of the reaction (Keq = 420 at 20°C, Reynolds et al., 1971) is strongly in favor of DHAP, so the complex of TIM with this substrate might be expected to be observable crystallographically. In conjunction with progress in the crystallographic structure determination of the native enzyme, studies of the complex of TIM with DHAP are being undertaken. Preliminary results show that direct observation of the interactions between enzyme and substrate in the crystal is complicated by changes in the molecular packing, but knowledge of the structure of the native enzyme will help interpret these results. TIM presents a system which offers a hitherto unequalled opportunity of studying the interaction of an enzyme with its true substrate at the...
A method for estimating wind-wave growth and decay in shallow water with high values of bottom friction (1977)

This report presents an approximate method for estimating wind-wave growth and decay over flooded areas where there is a major effect from bottom friction because of dense vegetation. (Author)
Reversible bioresponsive aptamer-based nanocomposites: ATP binding and removal from DNA-grafted silica nanoparticles (2013)

The bio-responsive reversible assembly of silica nanoparticles grafted with complementary DNA strands containing an ATP-sensitive aptamer sequence was studied. The optimal conditions for ATP-induced specific disassembly of the bionanocomposite network were identified, highlighting the existing competition between ATP/aptamer interactions and DNA duplex stability. A new, dialysis-based method for ATP removal was proposed, allowing partial re-formation of the initial DNA duplexes. Further disassembly was re-achieved by subsequent addition of ATP. These data constitute promising preliminary steps towards DNA-based fully reversible responsive bionanocomposite devices.
Thermal Twisting in Zinc Wire (1958)

Thermal Twisting in Zinc Wire When zinc wire recrystallized at a low temperature after cold-drawing is subjected to heating or cooling it twists in a function of temperature In thermal cycles in a given low temperaure range thehermal cycle twisting curves are linear and the cooling curves follow the heating curves If however the upPer cycling temperaure is heightened every cycle the cooling curves do not follow the heating curves and therefore permanent torsion results from the cyclic thermal treatrnen The heterogeneity of texture in wire has a remarkably strong influence in thermalwistingIt seems that the pronounced anisotropy of thermal expansion and preferred orientation in zinc play a signicant role in thermal twisting (