gebhart/patrepeval huggingface dataset:
G protein gene expression during mouse oocyte growth and maturation, and preimplantation embryo development (1996)

Fertilization in mammals initiates “egg activation,” a series of events leading to embryo development. The signal transduction events that occur as a result of sperm-egg interactions and that initiate egg activation may be analogous to a ligand-receptor-effector pathway, but the details of this signaling pathway are poorly understood. Several lines of evidence support a role for guanine nucleotide-binding regulatory proteins (G proteins) in mammalian egg activation. Prior to initiating studies to examine further the role of specific G proteins in sperm-induced mouse egg activation, we needed to define the complement of G proteins expressed in the egg. Using a reverse transcription-polymerase chain reaction (RT-PCR) assay, the relative levels of mRNAs encoding specific G protein α, β, and γ subunits were determined in meiotically incompetent oocytes, fully-grown competent oocytes, metaphase II-arrested eggs, one-, two-, and eight-cell embryos, and blastocysts. mRNA transcripts representing all of the heterotrimeric G protein families were present at all of the stages examined, and all underwent significant changes in their patterns of expression. The following heterotrimeric G protein mRNA transcripts were present in oocytes, eggs, or preimplantation embryos: Gαq family (q, 11, and 14), Gα12 family (12 and 13), Gαi family (i1, i2, i3, t2, z, and s), β subunits 1, 2, 4, and 5, and γ subunits 2, 3, 5, and 7. A recently described large molecular weight G protein, Gαh (Nakaoka et al., 1994: Science 264:1593–1596), was also present. Gα15, Gαt1, Gαolf, GαoA, Gβ3, Gγ1, and Gγ8 mRNA transcripts were not detected using this method. The most common pattern of expression observed was a maturation-associated decrease followed by an increase after the two-cell stage. Some transcripts, however, were expressed at low levels until the eight-cell to blastocyst stages, whereas others were expressed at high levels in the oocyte but following maturation declined and remained at a low level throughout preimplantation development. © 1996 Wiley-Liss, Inc.
In vitro activity of cefbuperazone against Bacteroides spp (1985)

The activity of cefbuperazone was tested in vitro against 287 clinical isolates of Bacteroides spp. Cefbuperazone showed good activity against B. fragilis, B. vulgatus, and other Bacteroides species, comparable to that of cefoxitin. It was relatively ineffective against B. distasonis and the B. thetaiotaomicron-ovatus group and was not active against cefoxitin-resistant Bacteroides spp.
Development of solar Stirling engine alternator for space experiments (2003)

A prototype solar Stirling cycle engine alternator has been constructed and tested. The flight model is scheduled to be tested in the mid-1990s on the second flight mission of the Space Flyer Unit (SFU). This prototype, designated IAS-200, is a scaled-down version (200 W of electric output power) of the flight model, but its construction is the same. The design of IAS-200 and the latest test results are discussed.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Surgical therapies, part II: flip-top transplants in vitiligo (2001)

ABSTRACT: The flip-top transplantation (FTT) method provides an important option for the treatment of depigmentation. It enables any physician to treat stable areas of depigmentation using equipment that is readily available and easy to use. To date, this method has resulted in the spread of pigmentation from each graft and has caused no visible scarring. FTT is best suited for treating localized areas of stable depigmentation where the cosmetic result is a priority.
3-regular three-XORSAT planted solutions benchmark of classical and quantum heuristic optimizers (2022)

Abstract With current semiconductor technology reaching its physical limits, special-purpose hardware has emerged as an option to tackle specific computing-intensive challenges. Optimization in the form of solving quadratic unconstrained binary optimization problems, or equivalently Ising spin glasses, has been the focus of several new dedicated hardware platforms. These platforms come in many different flavors, from highly-efficient hardware implementations on digital-logic of established algorithms to proposals of analog hardware implementing new algorithms. In this work, we use a mapping of a specific class of linear equations whose solutions can be found efficiently, to a hard constraint satisfaction problem (three-regular three-XORSAT, or an Ising spin glass) with a ‘golf-course’ shaped energy landscape, to benchmark several of these different approaches. We perform a scaling and prefactor analysis of the performance of Fujitsu’s digital annealer unit (DAU), the D-Wave advantage quantum annealer, a virtual MemComputing machine, Toshiba’s simulated bifurcation machine (SBM), the SATonGPU algorithm from Bernaschi et al , and our implementation of parallel tempering. We identify the SATonGPU and DAU as currently having the smallest scaling exponent for this benchmark, with SATonGPU having a small scaling advantage and in addition having by far the smallest prefactor thanks to its use of massive parallelism. Our work provides an objective assessment and a snapshot of the promise and limitations of dedicated optimization hardware relative to a particular class of optimization problems.
High Gradient Magnetic Separation Technique for Wastewater Treatment (1991)

Smit Nymegen Magnetic Water treatment Systems (MWS), a business group of Smit Transformatoren BV, has recently developed a very efficient magnet for wastewater treatment. The magnet is the core element of a complete system to eliminate phosphates, heavy metals and other pollutants from wastewater. The system is based on the attachment of wastewater pollutants to a magnetic carrier material (magnetite) and a subsequent magnetic separation of the magnetite-pollutant conglomerates. After separation, the magnetite is recovered and re-used in the process. Main advantages of the magnetic separation process are:▸high elimination performance▸low power input for magnet operation▸compact process▸low space requirements▸no clogging because of open matrix structure. The development of the magnetic system has been focused on the phosphate-removal from effluents of sewage treatment plants. Being a tertiary treatment, magnetic phosphate removal can be applied after any type of sewage treatment and it does not interfere with the biological process. Pilot plant tests at various sewage works showed that magnetic separation reduces the total-phosphate concentration to values as low as 0.1-0.5 mg/l. The system will be demonstrated in two full-scale plants with a capacity of 300 m3/h and 600 m3/h, respectively. The costs of magnetic phosphate removal (Dfl. 12,- to 18,-) are competitive with those of other phosphate removal techniques. The development of the magnetic phosphate removal system is granted by the Dutch Ministries of Housing, Physical Planning and Environment, Economic Affairs and Transport and Public Works.
Growth of permanent lymphoid cell cultures from human source: tenth anniversary. (1975)

The authors developed 52 permanent lymphoid cell cultures from various human sources, mainly from neoplastic lymphoproliferative disease. These cultures are reviewed against the background of similar cultures obtained in several other laboratories. The peculiar features of these cultures are: 1) the phenomenon of lymphoblastoid transformation; 2) the production of both globulins and mediators of delayed hypersensitivity; 3) the presence of herpes type virus as related to etiology or autoimmunity; and 4) cytotoxicity and other assays for the demonstration of tumor-specific antigens.
Anticancer activity of all-trans retinoic acid-loaded liposomes on human thyroid carcinoma cells B Biointerfaces (2017)

All-trans retinoic acid (ATRA) is an anti-tumor compound, exerting different anti-cancer effects on different types of cancer cells. Unfortunately, retinoids are also characterized by certain side effects following systemic administration, such as the burning of skin and general malaise. The highly variable degree of bioavailability of ATRA plus its tendency to induce its own destruction through metabolic degradation following oral treatment necessitate the development of alternative formulations. The aim of this work is to evaluate the physico-chemical properties of unilamellar, ATRA-containing liposomes and to investigate the cytotoxic activity of this potential nanomedicine on human thyroid carcinoma cells. Liposomes made up of DPPC/Chol/DSPE-mPEG2000 (6:3:1 molar ratio), characterized by a mean diameter of ∼200nm, a polydispersity index of 0.1 and a negative surface charge, were used as ATRA-carriers and their antiproliferative efficacy was investigated in comparison with the free drug on three different human thyroid carcinoma cell lines (PTC-1, B-CPAP, and FRO) through MTT-testing. The liposomes protected the ATRA against photodegradation and increased its antiproliferative properties due to the improvement of its cellular uptake. ATRA-loaded liposomes could be a novel formulation useful for the treatment of anaplastic thyroid carcinoma.
Corrigendum: Genetic Evidence for Transboundary Circulation of Peste Des Petits Ruminants Across West Africa (2019)

[This corrects the article DOI: 10.3389/fvets.2019.00275.].
Timing of Major Events of Pharyngeal Swallowing (1988)

Normal swallowing depends on the synergistic motion of the tongue, larynx, and pharyngeal wall to develop a bolus pressure gradient for bolus transit. However, few studies have provided timing relationships for clinical evaluations. To examine the timing relationships of pharyngeal anatomic motion, pressure generation, and bolus transit in the normal swallow, this study uses a new method, manofluorography, to correlate these swallowing aspects. Thirty-one events were timed by analyzing five swallows each in 14 normal subjects. A different perspective of pharyngeal physiology is presented.
EFFECTS OF THE NUCLEAR FACTOR-κB INHIBITORS 2-HYDROXY-4-TRIFLUOROMETHYLBENZOIC ACID AND ASPIRIN ON MICTURITION IN RATS WITH NORMAL AND INFLAMED BLADDER (2001)

The results show that HTB is devoid of significant effects on the micturition reflex in the absence or presence of bladder inflammation, suggesting that acute inhibition of nuclear factor-kappaB does not influence bladder urodynamics in rats. In contrast, aspirin, which is a cyclooxygenase and nuclear factor-kappaB inhibitor, was always effective, indicating the important role of cyclooxygenase enzymes.
Antibody quantitation in seconds using affinity Perfusion Chromatography. (1991)

An extremely rapid assay technique for antibodies has been developed utilizing protein A or protein G bound to Perfusion Chromatography support matrices. Either dilute or concentrated samples are directly injected on a column that selectively binds antibody, which is quantitated directly by elution and UV absorbance. Due to the unique mass transport characteristics of the supports, total assay cycle times are typically 1 minute or less, with assays as short as 15 seconds possible. The assay system can accurately quantitate a 100,000:1 or greater dynamic range in sample concentration without sample dilution, is extremely repeatable and is easy to automate with conventional HPLC systems. Assay of antibodies in a wide range of sample types has been demonstrated.
Magnetic, structural, and transport properties of thin film and single crystal Co2MnSi (2001)

The magnetic, structural, and transport properties of the Heusler alloy Co2MnSi are reported for sputtered thin films and a single crystal. X-ray diffraction reveals a phase pure L21 structure for all films grown between 573 and 773 K. Films grown at 773 K display a four-fold decrease in the resistivity relative to those grown at lower temperatures and a corresponding 30% increase in the residual resistivity ratio (ρ300 K/ρ5 K). We show that the higher growth temperature results in lattice constants, room temperature resistivities, and magnetic properties that are comparable to that of the bulk single crystal.
Radical Innovative CVT Technology: A Transmission Replacement Example (2003)

Currently the automotive CVT (Continuous Variable Transmission) market is dominated by CVT’s that work on a friction drive principle operating in a traction fluid. This paper presents a new CVT concept based on a unique mechanical joint unit. These units are used in parallel as an intermittent drive to produce a CVT. A mathematical model of the mechanical joint is derived and its suitability to operate within the CVT is discussed. The extension of the ratio range of the CVT via a planetary gear arrangement is demonstrated. The integration of the CVT with AMT (Automated Manual Transmission) technology is discussed and an example of such integration is presented. The efficiency of the system is estimated from related published information. In the summary the advantages of the CVT are discussed by comparison to traction fluid based CVT’s.
Oral herpes simplex virus infection with cardiac transplantation (1990)

Oral herpes simplex virus infection is a common complication of cardiac transplantation. Lesions are secondary to reactivation of the virus, are atypical in appearance, and can involve any oral and perioral surface. Diagnosis on clinical grounds is difficult and should be confirmed with laboratory testing. A case report and review of the literature are presented to support the features of this infection.
Field ionization of surface absorbates (1979)

When an auxiliary gas of low ionization potential, such as ${\mathrm{H}}_{2}$, is added to helium the energy spectrum of field-ionized helium ions displays a second spectral line of greater energy. This satellite line originates from the ionization of field-adsorbed He atoms following their excitation by the impact of electrons released during free-space ionization of the auxiliary gas. Using a magnetic-sector atom-probe field-ion microscope we have studied the field dependence of the difference in energies of the two spectral lines D(F), as a function of crystallographic plane, emitter material, and gas species. From our data and a simple expression for D(F) we have determined the position of the adsorbate with respect to the image plane and the ionic potential at the position of the adsorbate for the (111), (112), and (011) planes of tungsten. Results for Mo, Ir, and Re are also presented.
Numerical and experimental analysis of complex surface acoustic wave fields (2002)

Sophisticated high frequency signal processing as well as sensor applications based on surface acoustic waves (SAW) often require a complex transducer layout. We present a simple numerical technique based on a Huygens-type construction to calculate acoustic wave fields for an arbitrary given transducer layout. Approximating the slowness curve of different substrate materials by a parabolic function, we calculate the SAW far field with superposition of circular waves emitted by distributed point sources. Our numerical results for different transducer geometries are in excellent agreement with measurements of the acoustic wave field by an X-ray topographic technique.
Immobilized photocatalyst structure assuring optimal light distribution in a solar reactor (2014)

Abstract. Immobilized TiO2 photocatalyst with a high specific surface area was prepared on stainless steel woven meshes in order to be used packed in layers for water purification. Immobilization of such a complex shape needs a special coating technique. For this purpose, dip coating and electrophoretic deposition (EPD) techniques were used. The EPD technique gave the TiO2 coating films better homogeneity and adhesion, fewer cracks, and higher •OH formation than the dip coating technique. The woven mesh structure packed in layers guaranteed an efficient light-penetration in water treatment reactor. A simple equation model was used to describe the distribution of light through the mesh layers in the presence of absorbing medium (e.g., colored water with humic acids). Maximum three or four coated meshes were enough to harvest the solar UV light from 300 nm to 400 nm with a high penetration efficiency. The separation distance between the mesh layers played an important role in the efficiency of solar light penetration through the coated mesh layers, especially in case of colored water contaminated with high concentrations of humic acid.
Measurement and prevention of myocardial injury during percutaneous coronary intervention (2007)

See article on page 703
One to chelate them all: investigation of a versatile, bifunctional chelator for 64Cu, 99mTc, Re and Co (2011)

We describe the synthesis of the dip (di-picolyl-carboxylate) bifunctional chelator system, capable of fast coordination of Cu(2+), (64)Cu(2+) and Co(2+), as well as the [M(CO)(3)](+)-core (M = (99m)Tc, Re); it displays a variety of binding modes--tridentate when protected, tetradentate when deprotected. Syntheses of both the benzyl-nitro derivative and the benzyl-amino derivatives are described. The latter was coupled to biotin to show the viability of the system for functionalization with biomolecules. Besides coordination chemistry with stable isotopes, we also present labelling data with (64)Cu and (99m)Tc, as well as in vitro stability studies.
SOAP-binQ: high-performance SOAP with continuous quality management (2004)

There is substantial interest in using SOAP (simple object access protocol) in distributed applications' interprocess communications due to its promise of universal interoperability. The utility of SOAP is limited, however, by its inefficient implementation. Our research aims to make SOAP useful for high end or resource-constrained applications. The resulting SOAP-bin communication protocol exhibits substantially improved performance compared to regular SOAP communications. Gains are particularly evident when the same types of parameters are exchanged repeatedly, examples including transactional applications, remote graphics or visualization, and distributed scientific codes. A further improvement to SOAP-bin, termed SOAP-binQ, addresses resource-constrained applications like distributed media codes, where scarce communication bandwidth, for example, may prevent end users from interacting in real-time. SOAP-binQ offers quality management functions that permit SOAP to reduce parameter sizes dynamically, as and when needed. The methods used in size reduction are provided by end users and/or by applications, thereby enabling domain-specific tradeoffs in quality vs. performance. An adaptive use of SOAP-binQ's quality management techniques presented significantly reduces the jitter experienced in two sample applications like remote sensing and remote visualization.
Microbead Arrays for the Analysis of ErbB Receptor Tyrosine Kinase Activation and Dimerization in Breast Cancer Cells (2009)

Receptor tyrosine kinases (RTKs) in the ErbB family (EGFR, ErbB2, ErbB3, and ErbB4) are implicated in a variety of human malignancies. Accordingly, determination of both expression and activation (dimerization/heterodimerization and phosphorylation) of ErbB proteins is critical in defining their functional role in cancer. Efficient and comprehensive methods to study molecular functions of ErbB family of RTKs are needed not only for improvements in diagnostics but also for early screening of targeted drugs (eg, small molecule inhibitors and therapeutic antibodies). We report development of 3 multiplex microbead immunoassays for simultaneous detection of expression, protein-protein interactions, and phosphorylation of these RTKs. These novel multiplex immunoassays were used to study ErbB RTKs under different cell activation conditions in 2 breast cancer cell lines (MDA-MB-453 and MDA-MB-468) and an epidermoid cancer cell line (A431). The results were confirmed by immunoprecipitation/western blot. Importantly, the multiplex immunoassay facilitated time-course studies in these cell lines after cell activation with EGF and neuregulin, revealing the kinetics of phosphorylation of the ErbB family RTKs. This study demonstrates the utility of the Luminex(R) multiplex system as an efficient and comprehensive approach to study different aspects of molecular roles of these RTKs. Importantly, the study provides proof-of-concept for the utility of the multiplex microbead immunoassay approach for potential use in efficient, robust, and rapid screening of drugs, particularly those targeting functional aspects of these potent signaling molecules. In addition, the assays described here may be useful for cancer diagnostics and monitoring efficacy of therapy targeting the ErbB family of RTKs.
Pilot study comparing Kendall™ AMD antimicrobial foam dressing with ALLEVYN™ Ag hydrocellular antimicrobial dressing in venous leg ulcers (2014)

Infection is an important factor that delays wound healing, yet management of wound infection remains clinically challenging. This pilot study compared the clinical effectiveness of two antimicrobial foam dressings – Kendall™ AMD (Covidien; containing polyhexamethylene biguanide) and ALLEVYN™ Ag (Smith & Nephew; containing silver) – in terms of wound size reduction or complete healing of venous leg ulcers. Data analyses were conducted on 32 patients, with 34.38% of ulcers healing or re-epithelialising within the 12-week intervention phase – 63.64% from the Kendall AMD group (n=7) and 36.36% (n=4) from the ALLEVYN Ag group. Pain reduction was found to be greater in the Kendall AMD arm. Exudate levels were reduced during the study period. There is evidence from this study to suggest that Kendall AMD is as effective as ALLEVYN Ag, a widely used antimicrobial dressing, and is an efficient wound management product for hard-to-heal venous leg ulcers.
Relaxation Methods for Minimum Cost Ordinary and Generalized Network Flow Problems (1988)

We propose a new class of algorithms for linear cost network flow problems with and without gains. These algorithms are based on iterative improvement of a dual cost and operate in a manner that is reminiscent of coordinate ascent and Gauss-Seidel relaxation methods. We compare our coded implementations of these methods with mature state-of-the-art primal simplex and primal-dual codes, and find them to be several times faster on standard benchmark problems, and faster by an order of magnitude on large, randomly generated problems. Our experiments indicate that the speedup factor increases with problem dimension.