gebhart/patrepeval huggingface dataset:
Movement of embryonic chick sympathetic neurons on laminin in vitro is preceded by neurite extension (1993)

Chick sympathetic neurons (E-9) are capable of moving on a laminin substrate but not on more adhesive substrates in vitro. The effect of laminin is dose-dependent and reduced by the addition of anti-laminin antibodies, whereas soluble laminin does not stimulate movement. The onset of neuronal movement is preceded by, and highly correlated with, the onset of neurite formation. The addition of 1,2 dioctanoyl-sn-glycerol (DAG), a stimulator or protein kinase C that has been shown to inhibit neurite outgrowth, was found to delay both process formation and neuronal movement but did not affect the correlation between these two measures. These results support the conclusion that laminin stimulates primary neuronal movement in vitro and suggest that the mechanism underlying movement involves process formation followed by "towing" of the cell body by the advancing process. The similarities of this in vitro behavior to that observed in vivo suggest that similar mechanisms may underlie neuronal movement in the developing nervous system as suggested by Morest (Z Anat Entwicklungsgesch 130:265-305, 1970) and Liesi (EMBO J 4:1163-1170, 1985; Exp Neurol 117:103-113, 1992).
Near-field line-integral representation of the Kirchhoff-type aperture radiation for a parabolic reflector (2003)

A line-integral representation is presented for a linearly polarized Kirchhoff-type aperture radiation from a parabolic reflector antenna. The main purpose of this result is concerned with the acceleration of the numerical integration for calculating the near field of large reflector antennas. The formulation, which is rigorous for a uniform aperture field, is based on the application of the equivalence principle to a projecting surface, which allows the analytical evaluation in a closed form of a twofold surface integral which defines the radiated field at any space point; the extension to a slowly varying primary feed pattern is based on an asymptotic approximation, which is proved to be accurate in the proximity of the aperture to -30 dB of amplitude edge illumination. The present formulation is well suited to be improved by fringe diffraction contributions in the framework of edge-wave theories such as the physical theory of diffraction (PTD) and the incremental theory of diffraction (ITD).
Microbial Baeyer-Villiger Oxidation of Bicyclo[4.3.0]ketones by Two Recombinant E. coli Strains. A Novel Access to Indole Alkaloids (2002)

Recombinant Escherichia coli overexpressing Pseudomonas sp. NCIMB 9872 cyclopentanone monooxygenase (CPMO; E.C. 1.14.13.16) and Acinetobacter sp. NCIMB 9871 cyclohexanone monooxygenase (CHMO; E.C. 1.14.13.22) have been utilized in whole-cell biotransformations of prochiral bicyclo[4.3.0]ketones. The lactones produced in a biocatalytic Baeyer-Villiger oxidation represent key intermediates for the synthesis of several indole alkaloids. The two over-expression systems demonstrated a tendency for the formation of opposite enantiomers with CPMO giving (+)-lactones in good yields and excellent enantiomeric excess.
Poster - Wed Eve-54: Optimization of the MV Energy Spectrum from a Linear Accelerator for Improved Imaging (2009)

One of the successful approaches for improving megavoltage imaging using a linear accelerator is to replace the clinical target with a low-Z material and to remove the flattening filter. In recent work by Orton and Robar (Phys Med Biol. 54(5), 2009), energy spectra were generated using aluminum targets with thicknesses equal to 60% of the CSDA range of the electron. Targets were located above the linac carousel, with a 0.9 cm polystyrene absorber inserted in upper accessory slot to fully stop transmitted electrons. The current project aims to further improve the MV imaging spectrum through two approaches: i) by altering the thickness of the low-Z target and ii) reducing the energy of the electron beam. Through Monte Carlo (MC) simulation, we have found that full-thickness targets generate comparable photon energy spectra and less electron contamination. An electron beam with energy 3.5 MeV has been experimentally achieved by installing a custom-made program board into a Varian 2100EX linac to modify the bending magnet current. By implementing these changes, the normalized photon population in the 25–150 KeV range can be increased from 24.4% to 35.2%. By improving the energy spectrum and eliminating the need for an electron absorber, these enhancements should improve both the performance and practicality of low-Z imaging.
Faculty Opinions recommendation of Photoswitchable fluorescent crystals obtained by the photoreversible coassembly of a nucleobase and an azobenzene intercalator. (2019)

Self-assembled nucleobases, such as G-quartets or quadruplexes, have numerous applications, but light-responsive structures are limited to small, noncrystalline motifs. In addition, the assembly of the widely exploited azobenzene photochromic compounds can produce fluorescent crystals of extended dimensions but at the prize of sacrificing their photoswitchability. Here, we overcome inherent limitations of self-assembly with a new concept of supramolecular coassembly leading to materials with unprecedented properties. We show that the coassembly of guanosine monophosphate (GMP) with an azobenzene-containing DNA intercalator produces supramolecular crystals arranged through a combination of π-π, electrostatic, and hydrogen-bond interactions. The resulting crystals are 100 μm long, pH-sensitive, fluorescent, and can be photoreversibly disassembled/reassembled upon UV/blue irradiation. This allows us to perform operations such as dynamic photocontrol of a single-crystal growth, light-gated permeability in membrane-like materials, and photoswitchable fluorescence. We believe this concept critically expands the breadth of multifunctional materials attainable by self-assembly. PMID: 31117648
Alzheimer's disease and apolipoprotein e‐4 allele in an amish population (1996)

Alzheimer's Disease (AD) is a complex genetic disorder with four loci already identified. Mutations in three of these, the amyloid precursor protein, presenilin I, and presenilin II, cause early-onset AD. The apolipoprotein E (APOE) gene contributes primarily to late-onset AD. The APOE-4 allele acts in a dose-related fashion to increase risk and decrease the age-of-onset distribution in AD. We examined the effect of APOE on AD in a previously unstudied Amish population that has a lower prevalence of dementia compared with other populations. We sampled a large inbred family with 6 late-onset AD members. We also genotyped 53 individuals from the general Amish population as controls for the APOE allele frequency estimates. The frequency of the APOE-4 allele in the Amish controls was 0.037 +/- 0.02. This differed significantly compared with three independent sets of non-Amish white controls (p < 2 x 10(-4), p < 6 x 10(-5), and p < 2 x 10(-6)). In addition, all Amish AD-affected individuals had APOE 3/3 genotypes; no APOE X/4 or 4/4 individuals were observed. We suggest that the lower frequency of dementia in the Amish may be partially explained by the decreased frequency of the APOE-4 allele in this population, and that the inbred nature of this pedigree, with its strong clustering of cases contrasted against the lower frequency of dementia, indicates that additional genetic factors influence late-onset AD.
Color array in TFA technology (2002)

A novel color image sensor array based on TFA (Thin Film on ASIC) technology is described. In the array a-Si(C):H nipin diodes act as optical three color detectors, whereas the crystalline circuit provides photo current integration and signal readout. In this way a single pixel sequentially generates a full color RGB signal without the need of color filters. The transient response of the diode under pulsed illumination is examined in order to evaluate the impact of the detector speed on sensor performance and image quality. By proper design of the pixel electronics and technological means the full dynamic range of the color diode can be exploited in the array.
The frequency of patients with dystrophin abnormalities in a limb‐girdle patient population (1991)

Of the 3,048 diagnostic muscle biopsies processed by the National Institute of Neuroscience, Tokyo, over 12 years, 41 cases carried the clinical diagnosis of limb-girdle muscular dystrophy. We have analyzed all 41 cases for dystrophin content in muscle by both immunofluorescence and immunoblot. We identified five male patients with an abnormal dystrophin pattern diagnostic of Becker muscular dystrophy, and two female patients with dystrophin patterns consistent with a manifesting carrier of Duchenne muscular dystrophy diagnosis. Thus, 17% of our limb-girdle patients showed a dystrophinopathy, indicating that they in fact had a disorder related to Duchenne/Becker muscular dystrophy. Misclassification of isolated male limb-girdle patients was 31% (4/13), while misclassification of isolated female limb-girdle patients was 13% (2/15). Using multiplex polymerase chain reaction analyses of small amounts of muscle biopsy DNA confirmed a dystrophin gene deletion in all five male Becker dystrophy patients identified. This study emphasizes the clinical overlap between limb-girdle muscular dystrophy and dystrophinopathies, and reinforces the necessity of dystrophin protein and gene studies for the accurate clinical diagnosis of isolated cases of muscular dystrophy.
Dendritic Cell Deficiency Associated With Development of BK Viremia and Nephropathy in Renal Transplant Recipients (2009)

Our results identify PBDC deficiency as a previously unrecognized risk factor for BKV reactivation after renal transplantation. Pretransplant PBDC monitoring may prove to be a useful clinical tool in the assessment of patient vulnerability to BKVN posttransplant, which may allow more focused screening.
A novel DC measurement method for the accurate extraction of bipolar resistive parasitics (2002)

A novel DC method for determining the components of series resistance in bipolar transistors is presented. As a DC technique, it shows unprecedented accuracy as demonstrated by its application to both metal-contacted heterojunction transistors and more conventional bipolar junction transistors. The measurement error was minimized by using a single double-base Kelvin-tapped transistor to extract all components of series resistance. The present work indicates that past methods for calculating the bias dependence of series resistances in bipolar devices are incorrect. Therefore, a correct expression for the bias-dependent intrinsic base resistance is also presented.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Richtlijn 'Perinataal beleid bij extreme vroeggeboorte' (2010)

At the request of the State Secretary of the Dutch Ministry of Health, Welfare and Sport a national multidisciplinary workgroup developed an evidence-based practice guideline for the management of pregnant women with an imminent preterm delivery after a pregnancy of less than 26 weeks duration and for extremely preterm neonates. Active care measures are advised for neonates from a gestational age of 24 0/7 weeks onwards, unless there are serious arguments that justify a conservative management. In cases of imminent preterm delivery, intrauterine transport to a perinatological care centre is advised from a gestational age of 23 4/7 weeks onwards. In cases of imminent preterm delivery, glucocorticoids to enhance fetal lung maturity should be administered from a gestational age of 23 5/7 weeks onwards. From a gestational age of 24 0/7 weeks onwards a caesarean section may be considered if the fetal condition during spontaneous labour justifies this
The Role of Intra- and Extragranular Microcrystalline Cellulose in Tablet Dissolution (1996)

The objective of this study was to examine the influence of intra- and extragranular microcrystalline cellulose (MCC) on drug dissolution from tablets made by high-shear granulation. Granulations were made in a Littleford Model W-10-B (10-liter) mixer and dried in a fluid bed dryer (Niro Inc.). A Plackett-Burman screening design and 2(3) factorial design were employed to study how drug type, MCC (intra- or extra-), filler type (lactose or dicalcium phosphate), disintegrant type (sodium starch glycolate or croscarmellose sodium) and level, proportion of magnesium stearate, and impeller speed affect tablet hardness, disintegration time, and dissolution. Two model drugs were chosen based on their solubility: metoprolol tartrate (solubility > 1000 mg/ml) and hydrochlorothiazide (solubility = 1.05 mg/ml). Tablets were compressed to the same target weight (dose) and similar tablet hardness. In some cases, dissolution testing was also carried out on the loose granules. The intra-extragranular distribution of MCC was found critical to the compactibility and initial dissolution rates from these tablets. Intragranular MCC reduced drug dissolution, the effect being most marked in the case of the slightly soluble hydrochlorothiazide. For formulations containing intragranular MCC, the granulating fluid level on tablet dissolution was also important, since an increase in fluid level resulted in slower drug dissolution from both the loose granules and the tablets compressed from them. Conversely, extragranular MCC tended to increase both dissolution rates and compactibility. It may be concluded that the appropriate distribution of MCC between and within granules may optimize both dissolution and compactibility without changing overall tablet composition.
Cytotoxic and cytostatic effects of the streptococcal preparation OK-432 and its subcellular fractions on human ovarian tumor cells (1989)

Previous studies have indicated that OK-432 is a potent biologic response modifier (BRM) and that it augments immune responses to tumor cells. We studied the direct effect of OK-432 on tumor cells. Established and freshly derived human ovarian carcinoma lines were examined for their susceptibility to OK-432 or its subcellular fractions in direct cytotoxicity, cytostatic activity, and inhibition of metabolic activity. OK-432 was cytotoxic to 13 of 15 freshly derived ovarian carcinoma lines in a 24-hour chromium-51 (51Cr) release assay. The optimal effect was noticed at OK-432 concentrations between 0.1 and 1.0 Klinishe Einhert (KE) per milliliter. The cytostatic effect on two established lines and one fresh line correlated with the cytotoxic activity. In all three lines, however, the metabolic activities (DNA, RNA, and protein synthesis) were inhibited by OK-432, suggesting that cell lysis by OK-432 may not be directly correlated with the inhibition of metabolic activities. Several subcellular fractions were derived from OK-432 and only the cytoplasmic and protoplast membrane fractions showed cytotoxic activity against the OK-432-sensitive tumor cell lines, although the cytotoxicity obtained was greatly less than the whole microorganism OK-432. The direct binding of 14C-OK-432 to tumor cells was examined. Binding took place rapidly after 1 hour of incubation and reached a maximum activity at 37 degrees C. Binding in all three lines ranged between 1.7 and 2.7 pg/cell. These results demonstrate the direct cytotoxic effect of OK-432 and some subcellular fractions on human ovarian carcinoma lines. These results also show that the BRM OK-432 may exert its effect by both potentiating the antitumor response and directly inhibiting tumor cell growth.
Effect of Pretransplant Preconditioning by Whole Body Hyperthermia on Islet Graft Survival (2007)

Previous observations in heat-shocked pig islets revealed the ambivalent character of the stress response simultaneously inducing processes of protection and apoptosis. To clarify whether the proapoptotic character of the stress response is reduced in heat-exposed islets still embedded in their native environment, hyperthermia was performed in the present study either as whole body hyperthermia (WBH) prior to pancreas resection or as in vitro heat shock (HS) after isolation. HS (42 degrees C/45 min) was induced in donors 12 h before isolation (WBH, n = 32) or in freshly isolated islets prior to 12 h of culture at 37 degrees C (in vitro HS, n = 25). Islets continuously incubated at 37 degrees C served as controls (n = 34). Proinflammatory treatment was performed with H2O2, DETA-NO, or a combination of IL-1beta, TNF-alpha, and IFN-gamma. Quality assessment included islet yield, viability staining, static glucose incubation, and nude mouse transplantation. WBH was significantly less effective than in vitro HS to induce HSP70 overexpression and to increase islet resistance against inflammatory mediators. Although characterized by an unaltered Bax to Bcl-2 ratio, islets subjected to WBH partially failed to restore sustained normoglycemia in diabetic nude mice. The inflammatory response observed in the pancreas of WBH-treated rats was associated with significantly reduced viability that seems to have a higher predictive value for posttransplant outcome compared to islet in vitro function or mitochondrial activity. In contrast, in vitro HS significantly decreased transcript levels of Bcl-2, but did not affect posttransplant function compared to sham-treated islets. These findings suggest that WBH is primarily associated with increased necrosis as a secondary tissue type-specific effect of pancreas damage while in vitro HS mainly induces apoptosis.
Expanded Surface Area Fibers: A Means for Medical Product Enhancement (2001)

Expanded surface area fibers in the 4DG geometry provide about three times the amount of specific surface per denier compared to circular fibers. Fabrics made from these fibers provide greater loft, fluid movement, and particle capture than traditional woven, knitted, or nonwoven structures. This paper includes a description of these fibers and a listing of medical product applications that might benefit from their incorporation.
Das Verhalten der Rückstromdrossel (Wirbelkammerdiode) beim Druckwellendurchgang (1998)

In sterreich wurde in einigen alpinen Speicherkraftwerken zur Dmpfung der Wasserschloschwingungen eine sogenannte Rckstramdrossel am Ende der unteren Kammer im bergang zum Steigschacht des Wasserschlosses eingebaut.Ihre Form hnelt dem Spiralgehuse einer Francisturbine mit geradem Saugrohr.Durch Regel-
Investigation of Possible Solvents for Extracellular Polysaccharides from Streptococcus mutans and Streptococcus sanguis (1975)

The solubilities of extracellular polysaccharide fractions produced by 14 oral streptococcal strains were compared in water, aqueous lithium and guanidine salt solutions, dimethyl sulfoxide (DMSO), and a 9:1 DMSO-water mixture. The best results for solubilizing the fractions were obtained with the DMSO-water mixture procedure, which used the water and DMSO in sequence. By this method all fo the fractions were solubilized.
Satellite and Correlative Measurements of the Stratospheric Aerosol. II: Comparison of Measurements Made by SAM II, Dustsondes and an Airborne Lidar (1981)

We show results from the first set of measurements conducted to validate extinction data from the satellite sensor SAM II. Dustsonde-measured number density profiles and lidar-measured backscattering profiles for two days are converted to extinction profiles using the optical modeling techniques described in the companion Paper I (Russell et al., 1981). At heights ∼2 km and more above the tropopause, the dustsonde data are used to restrict the range of model size distributions, thus reducing uncertainties in the conversion process. At all heights, measurement uncertainties for each sensor are evaluated, and these are combined with conversion uncertainties to yield the total uncertainty in derived data profiles. The SAM II measured, dustsonde-inferred, and lidar-inferred extinction profiles for both days are shown to agree within their respective uncertainties at all heights above the tropopause. Near the tropopause, this agreement depends on the use of model size distributions with more relatively large particles (radius ≳0.6 μm) than are present in distributions used to model the main stratospheric aerosol peak. The presence of these relatively large particles is supported by measurements made elsewhere and is suggested by in situ size distribution measurements reported here. These relatively large particles near the tropopause are likely to have an important bearing on the radiative impact of the total stratospheric aerosol. The agreement in this experiment supports the validity of the SAM II extinction data and the SAM II uncertainty estimates derived from an independent error analysis. Recommendations are given for reducing the uncertainties of future correlative experiments.
Effective intercellular communication distances are determined by the relative time constants for cyto/chemokine secretion and diffusion (1997)

A cell's ability to effectively communicate with a neighboring cell is essential for tissue function and ultimately for the organism to which it belongs. One important mode of intercellular communication is the release of soluble cyto- and chemokines. Once secreted, these signaling molecules diffuse through the surrounding medium and eventually bind to neighboring cell's receptors whereby the signal is received. This mode of communication is governed both by physicochemical transport processes and cellular secretion rates, which in turn are determined by genetic and biochemical processes. The characteristics of transport processes have been known for some time, and information on the genetic and biochemical determinants of cellular function is rapidly growing. Simultaneous quantitative analysis of the two is required to systematically evaluate the nature and limitations of intercellular signaling. The present study uses a solitary cell model to estimate effective communication distances over which a single cell can meaningfully propagate a soluble signal. The analysis reveals that: (i) this process is governed by a single, key, dimensionless group that is a ratio of biological parameters and physicochemical determinants; (ii) this ratio has a maximal value; (iii) for realistic values of the parameters contained in this dimensionless group, it is estimated that the domain that a single cell can effectively communicate in is approximately 250 micron in size; and (iv) the communication within this domain takes place in 10-30 minutes. These results have fundamental implications for interpretation of organ physiology and for engineering tissue function ex vivo.
Four- to twenty-four-hour uptake ratio: an index of rapid iodine-131 turnover in hyperthyroidism. (1996)

The 4- to 24-hr 131I thyroidal uptake ratio is a practical substitute for exact determination of the effective half-life. It identifies patients who are likely to have a rapid 131I turnover without the need for extended thyroid uptake measurements. An 131I uptake ratio of > or = 1 was found in 15% of hyperthyroid patients and was associated with a near 50% 131I therapy failure rate.