gebhart/patrepeval huggingface dataset:
N-Formyl Peptide Receptors Internalize but Do Not Recycle in the Absence of Arrestins (2003)

Arrestins mediate phosphorylation-dependent desensitization, internalization, and initiation of signaling cascades for the majority of G protein-coupled receptors (GPCRs). Many GPCRs undergo agonist-mediated internalization through arrestin-dependent mechanisms, wherein arrestin serves as an adapter between the receptor and endocytic proteins. To understand the role of arrestins in N-formyl peptide receptor (FPR) trafficking, we stably expressed the FPR in a mouse embryonic fibroblast cell line (MEF) that lacked endogenous arrestin 2 and arrestin 3 (arrestin-deficient). We compared FPR internalization and recycling kinetics in these cells to congenic wild type MEF cell lines. Internalization of the FPR was not altered in the absence of arrestins. Since the FPR remains associated with arrestins following internalization, we investigated whether the rate of FPR recycling was altered in arrestin-deficient cells. While the FPR was able to recycle in the wild type cells, receptor recycling was largely absent in the arrestin double knockout cells. Reconstitution of the arrestin-deficient line with either arrestin 2 or arrestin 3 restored receptor recycling. Confocal fluorescence microscopy studies demonstrated that in arrestin-deficient cells the FPR may become trapped in the perinuclear recycling compartment. These observations indicate that, although the FPR can internalize in the absence of arrestins, recycling of internalized receptors to the cell surface is prevented. Our results suggest a novel role for arrestins in the post-endocytic trafficking of GPCRs.
Influence of age and renal impairment on the steady state pharmacokinetics of filgotinib, a selective JAK1 inhibitor (2018)

Age and mild to moderate impairment of renal function had limited impact on the PK of filgotinib. In subjects with severe RI, the exposure to the metabolite of filgotinib was elevated, consistent with its renal elimination pathway.
Advances in U.S. Heavy Ion Fusion Science (2007)

During the past two years, the US heavy ion fusion science program has made significant experimental and theoretical progress in simultaneous transverse and longitudinal beam compression, ion-beam-driven warm dense matter targets, high-brightness beam transport, advanced theory and numerical simulations, and heavy ion target physics for fusion. First experiments combining radial and longitudinal compression {pi} of intense ion beams propagating through background plasma resulted in on-axis beam densities increased by 700X at the focal plane. With further improvements planned in 2008, these results enable initial ion beam target experiments in warm dense matter to begin next year. They are assessing how these new techniques apply to higher-gain direct-drive targets for inertial fusion energy.
Hot flashes are not predictive for serum concentrations of tamoxifen and its metabolites (2013)

No univariate associations were observed between concentrations of active tamoxifen metabolites and either the frequency or severity of hot flashes during treatment. However, the frequency of hot flashes may be exacerbated by higher serum concentrations of tamoxifen and its metabolites in post-menopausal women with a history of hot flashes prior to tamoxifen treatment.
BSCCO-2212 Wire and Cable Studies (2011)

BSCCO-2212 round wires and cables are being studied for possible use in very high field magnets (>; 25 T) within the multi-Lab Very High Field Superconducting Magnet Collaboration (VHFSMC). Billets produced by Oxford Superconducting Technology were used to make Rutherford-type cables of various geometries. The effect of the cable parameters was measured on the extracted strand performance and deformation. Transport properties were measured at 4.2 K in fields up to 14 T for the extracted strands across the edges, and compared with as-received round wire. The same wire used in the cables was also deformed by flat rolling to reproduce the deformation seen by the round strand when formed into a Rutherford cable. After heat treatment, rolled samples were characterized for transport properties and results compared to those obtained for strands extracted from the cables. Finally cables were tested at 4.2 K and self-field using a superconducting transformer and results compared to those of the extracted strands.
Determination of the Contact Energies between a Regulator of G Protein Signaling and G Protein Subunits and Phospholipase Cβ<sub>1</sub> (2000)

Cell signaling proteins may form functional complexes that are capable of rapid signal turnover. These contacts may be stabilized by either scaffolding proteins or multiple interactions between members of the complex. In this study, we have determined the affinities between a regulator of G protein signaling protein, RGS4, and three members of the G protein-phospholipase Cbeta (PLC-beta) signaling cascade which may allow for rapid deactivation of intracellular Ca(2+) release and activation of protein kinase C. Specifically, using fluorescence methods, we have determined the interaction energies between the RGS4, PLC-beta, G-betagamma, and both deactivated (GDP-bound) and activated (GTPgammaS-bound) Galpha(q). We find that RGS4 not only binds to activated Galpha(q), as predicted, but also to Gbetagamma and PLCbeta(1). These interactions occur through protein-protein contacts since the intrinsic membrane affinity of RGS4 was found to be very weak in the absence of the protein partner PLCbeta(1) or a lipid regulator, phosphatidylinositol-3,4,5 trisphosphate. Ternary complexes between Galpha(q), Gbetagamma and phospholipase Cbeta(1) will form, but only at relatively high protein concentrations. We propose that these interactions allow RGS4 to remain anchored to the signaling complex even in the quiescent state and allow rapid transfer to activated Galpha(q) to shut down the signal. Comparison of the relative affinities between these interacting proteins will ultimately allow us to determine whether certain complexes can form and where signals will be directed.
Quantum dot resonant cavity photodiode with operationnear 1.3 µm wavelength (1997)

It is shown that strained-layer InGaAs quantum dots grown on GaAs substrates can extend the operating wavelength of GaAs-based optoelectronic devices to wavelengths near 1.3 µm. Specifically, a 1.27 µm resonant-cavity photodiode with a quantum dot absorbing region is demonstrated. This photodiode exhibits a peak external quantum efficiency of 49% with a spectral bandwidth of 1.2 nm.
Optically controlled coplanar waveguide millimeter wave phase shifter (1985)

A new optically controlled millimeter wave phase shifter is discussed. The device is based on the interaction between the guided wave on a coplanar waveguide (CPW) and an optically induced electron-hole plasma in the CPW semiconductor substrate. A prototype structure consisting of a Schottky-contacted coplanar waveguide on a multilayer AlGaAs/GaAs substrate is modeled. Optically-induced electron-hole plasma densities are calculated using the nonlinear diffusion equation, valid for high level injection in semiconductors. Numerical calculations of the phase shift and insertion loss versus illumination intensity are found based on these plasma densities.
Synthesis and Color Tuning of New Fluorene-Based Copolymers (2002)

A series of copolymers, poly{9,9-bis(2‘-ethylhexyl)fluorene-2,7-diyl-co-2,5-bis(2-thienyl-1-cyanovinyl)-1-(2‘-ethylhexyloxy)-4-methoxybenzene-5‘ ‘,5‘ ‘‘-diyl} (PFTCVB), were synthesized from the monomers 2,7-dibromo-9,9-bis(2‘-ethylhexyl)fluorene and 2,5-bis(2-(5‘-bromothienyl)-1-cyanovinyl)-1-(2‘ ‘-ethylhexyloxy)-4-methoxybenzene (BTCVB) through the Ni(0)-mediated polymerization. The copolymers were characterized using FT-IR spectroscopy, UV−vis spectroscopy, TGA, photoluminescence (PL) and electroluminescence (EL) spectroscopy, elemental analysis, and molecular weight studies. The synthesized copolymers showed an absorption maximum at about 380 nm, and between 425 and 600 nm the absorption increased with increasing fraction of thiophene-containing monomer 4 (BTCVB). In PL, the emission maxima of the copolymers were red-shifted as the fraction of BTCVB increases, despite the copolymers showing little variation in UV−vis absorption characteristics. Light-emitting devices were fabricated in an ITO (indium−tin oxide)/PEDOT/polymer/LiF/Al configuration. The EL spectra showed similar emissions to the PL results, and the copolymer containing 15 mol % of BTCVB showed bright-red emission.
The effect of parathyroid hormone (PTH) and 24,25-dihydroxy-vitamin D <sub>3</sub> on adenylyl cyclase of iliac crest biopsies: Diagnostic and prognostic tool for evaluation and treatment of uremic patients (1987)

The bone adenylyl cyclase (AC) complex of iliac crest biopsies of normals, uremic patients and subjects with primary hyperparathyroidism (PrHPT) have been investigated. Bone resorption (RS) in uremic patients appears to be related partly to increased serum parathyroid hormone (s-PTH) levels and to netto PTH-stimulated AC (net PTH-AC) and partly to the uremic condition (as estimated by s-Creatinine) per se. Serum PTH is able to completely desensitize the PTH dependent bone AC in normals in vivo, but only partially in uremic patients. In patients with PrHPT, the bone AC appears to be inert to homologous desensitization. Positive aluminum staining is associated with blunted CT-responsive and low basal AC. In the combined group of normals and uremic patients, net PTH-AC is (as predicted from human in vitro data and the rat model) inversely related to serum 24,25-diOH-D3. Net PTH-AC, when corrected for s-24,25-diOH-D3 levels, correlated well with RS. The described action of 24,25-diOH-D3 presents a clearly defined rationale for the use of 24,25-diOH-D3 concurrently with 1,25-diOH-D3 to treat renal osteodystrophy: By administering 1,25-diOH-D3, s-Ca2+ and s-PTH will normalize and consequently net PTH-AC diminish. 24,25-diOH-D3 is then believed to further reduce net PTH-AC and RS. A concomitant alleviation of the uremic condition would eventually ensure the fastest possible restoration of bone structure and function.
ChemInform Abstract: INSERTION REACTIONS OF DIAZO COMPOUNDS WITH SOME SELENIUM(II) ELECTROPHILES (1985)

Abstract Die Reaktion der Diazoverbindungen (II) mit den Selen‐haltigen Elektrophilen (I) bzw. (V) liefert die Insertionsprodukte (III), (IV) bzw. (VI).
Modeling the Raman Spectrum of Graphitic Material in Rock Samples with Fluorescence Backgrounds: Accuracy of Fitting and Uncertainty Estimation (2014)

We propose a robust technique called Savitzky-Golay second-derivative (SGSD) fitting for modeling the in situ Raman spectrum of graphitic materials in rock samples such as carbonaceous chondrite meteorites. In contrast to non-derivative techniques, with assumed locally linear or nth-order polynomial fluorescence backgrounds, SGSD produces consistently good fits of spectra with variable background fluorescence of any slowly varying form, without fitting or subtracting the background. In combination with a Monte Carlo technique, SGSD calculates Raman parameters (such as peak width and intensity) with robust uncertainties. To explain why SGSD fitting is more accurate, we compare how different background subtraction techniques model the background fluorescence with the wide and overlapping peaks present in a real Raman spectrum of carbonaceous material. Then, the utility of SGSD is demonstrated with a set of real and simulated data compared to commonly used linear background techniques. Researchers may find the SGSD technique useful if their spectra contain intense background interference with unknown functional form or wide overlapping peaks, and when the uncertainty of the spectral data is not well understood.
Resonant transformer command charging system for high repetition rate rare-gas halide lasers (1983)

A resonant transformer pulse generator for the pumping of high repetition rate rare-gas halide discharge lasers is described. Since with this design a low-voltage dc power supply is needed, command charging can easily be achieved by using a thyristor as the switching element. The pulse generator has been tested driving a thyratron-switched laser operating up to 100 pps free running and up to 200 pps in the burst mode.
Near‐ and mid‐infrared microspectroscopy of the Ronda peridotite (2006)

Microspectroscopy is a potentially useful tool for planetary applications, as individual mineral grains can be targeted and measured in situ. A series of closely coupled experiments was designed to investigate and compare the laboratory measurement of optical properties of a peridotite sample in the macroscale and microscale. The Ronda peridotite consists largely of olivine with significant orthopyroxene and minor clinoproxene and serpentine. Microscopic transmission spectra of unmounted thin sections produce strong, well‐defined absorption features in the near infrared, and band positions can be directly compared to macroscopic and microscopic reflectance spectra. For near‐infrared reflectance, where photons are multiply scattered by different grains, measurement of a microscopic target means most photons are scattered out of the field of view and few photons return to the detector from the target area. This precludes most microspectroscopic reflectance measurements of small features in the near infrared. In the mid‐infrared, where reflectance measurements involve only surface interactions, microspectroscopic measurements detect well‐defined absorption features for most samples. Although the intensity of absorption features varies between grains, band positions are consistent with macroscopic diffuse reflectance and emittance measurements of bulk samples, and can be used to extract general chemical information such as iron/magnesium ratios of olivines.
Joint planning of soft-capacity and soft-coverage for 3G WCDMA systems (2001)

A number of direction-specific issues on soft coverage analysis and planning along with capacity evaluation and planning, and also in resource management for third generation (3G) systems are discussed. The emphasis is on the differences between the approach in the uplink with that in the downlink.
Engineering of a glycosidase Family 7 cellobiohydrolase to more alkaline pH optimum: the pH behaviour of Trichoderma reesei Cel7A and its E223S/ A224H/L225V/T226A/D262G mutant (2001)

The crystal structures of Family 7 glycohydrolases suggest that a histidine residue near the acid/base catalyst could account for the higher pH optimum of the Humicola insolens endoglucanase Cel7B, than the corresponding Trichoderma reesei enzymes. Modelling studies indicated that introduction of histidine at the homologous position in T. reesei Cel7A (Ala(224)) required additional changes to accommodate the bulkier histidine side chain. X-ray crystallography of the catalytic domain of the E223S/A224H/L225V/T226A/D262G mutant reveals that major differences from the wild-type are confined to the mutations themselves. The introduced histidine residue is in plane with its counterpart in H. insolens Cel7B, but is 1.0 A (=0.1 nm) closer to the acid/base Glu(217) residue, with a 3.1 A contact between N(epsilon2) and O(epsilon1). The pH variation of k(cat)/K(m) for 3,4-dinitrophenyl lactoside hydrolysis was accurately bell-shaped for both wild-type and mutant, with pK(1) shifting from 2.22+/-0.03 in the wild-type to 3.19+/-0.03 in the mutant, and pK(2) shifting from 5.99+/-0.02 to 6.78+/-0.02. With this poor substrate, the ionizations probably represent those of the free enzyme. The relative k(cat) for 2-chloro-4-nitrophenyl lactoside showed similar behaviour. The shift in the mutant pH optimum was associated with lower k(cat)/K(m) values for both lactosides and cellobiosides, and a marginally lower stability. However, k(cat) values for cellobiosides are higher for the mutant. This we attribute to reduced non-productive binding in the +1 and +2 subsites; inhibition by cellobiose is certainly relieved in the mutant. The weaker binding of cellobiose is due to the loss of two water-mediated hydrogen bonds.
Role of Pore Size Location in Determining Bacterial Activity during Predation by Protozoa in Soil (1995)

The predation of a luminescence-marked strain of Pseudomonas fluorescens by the soil ciliate Colpoda steinii was studied in soil microcosms. Bacterial cells were introduced in either small (neck diameter, <6 (mu)m) or intermediate-sized (neck diameter, 6 to 30 (mu)m) pores in the soil by inoculation at appropriate matric potentials, and ciliates were introduced into large pores (neck diameter, 30 to 60 (mu)m). Viable cell concentrations of bacteria introduced into intermediate-sized pores decreased at a greater rate than those in small pores, with reductions in bacterial populations being accompanied by an increase in viable cell numbers of the ciliate. The data indicate that the location of bacteria in small pores provides significant protection from predation. In the absence of C. steinii, the level of metabolic activity of the bacterial population, measured by luminometry, decreased at a greater rate than cell number, and the level of luminescence cell(sup-1) consequently decreased. The decrease in levels of luminescence indicates a loss of activity due to starvation. During predation by C. steinii, the level of the activity of cells introduced into small pores fell in a similar manner. The level of cell activity was, however, significantly greater for cells introduced into intermediate-sized pores, despite their greater susceptibility to predation. The data suggest that increased activity arises from a release of nutrients by the predator and the greater accessibility of bacteria to nutrients in larger pores. Nutrient amendment of microcosms resulted in increases in bacterial populations to sustained, higher levels, while levels of luminescence increased transiently. The predation of cells introduced into intermediate-sized pores was greater, and there was also evidence that the level of activity of surviving bacteria was greater for bacteria in intermediate-sized but not small pores.
Stem Cell Therapies for the Treatment of Radiation-Induced Normal Tissue Side Effects (2013)

Follow-up studies will need to identify the best possible stem cell types for the treatment of early and late radiation-induced normal tissue injury. Additional work should seek to optimize cellular dosing regimes, identify the best routes of administration, elucidate optimal transplantation windows for introducing cells into more receptive host tissues, and improve immune tolerance for longer-term engrafted cell survival into the irradiated microenvironment.
Determination of trace transition elements in solids by proton induced x-ray emission analysis (1977)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDetermination of trace transition elements in solids by proton induced x-ray emission analysisArthur. Mittler, Barry K. Barnes, Robert. Litman, Frantz. Holton, and Eugene F. BarryCite this: Anal. Chem. 1977, 49, 3, 432–435Publication Date (Print):March 1, 1977Publication History Published online1 May 2002Published inissue 1 March 1977https://pubs.acs.org/doi/10.1021/ac50011a026https://doi.org/10.1021/ac50011a026research-articleACS PublicationsRequest reuse permissionsArticle Views20Altmetric-Citations6LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Quantitative absorption cytometry for measuring red blood cell hemoglobin mass and volume (2014)

We present an optical system, called the quantitative absorption cytometer (QAC), to measure the volume and hemoglobin mass of red blood cells flowing through a microfluidic channel. In contrast to clinical hematology analyzers, where cells are sphered in order for both volume and hemoglobin to be measured accurately, the QAC measures cells in their normal physiological shape. Human red blood cells are suspended in a refractive index-matching absorbing buffer, driven through a microfluidic channel, and imaged using a transmission light microscope onto a color camera. A red and a blue LED illuminate cells and images at each color are used to independently retrieve cell volume and hemoglobin mass. This system shows good agreement with red blood cell indices retrieved by a clinical hematology analyzer and in fact measures a smaller coefficient of variation of hemoglobin concentration. In addition to cell indices, the QAC returns height and mass maps of each measured cell. These quantitative images are valuable for analyzing the detailed morphology of individual cells as well as statistical outliers found in the data. We also measured red blood cells in hypertonic and hypotonic buffers to quantify the correlation between volume and hemoglobin mass under osmotic stress. Because this method is invariant to cell shape, even extremely nonspherical cells in hypertonic buffers can be measured accurately.
An operational approach for aircraft crew dosimetry: the SIEVERT system (2007)

International audience
An ant colony system minimizing total tardiness for hybrid job shop scheduling problem with sequence dependent setup times and release dates (2005)

International audience
Biochemical studies on fatty liver. VIII. Changes of .ALPHA.-glycerophosphate dehydrogenase activity in fatty liver of rats by amino acid imbalance. (1976)

In the previous reports,1,2) lipogenesis in fatty liver of
Developing Induced Pluripotent Stem Cell-Based Therapy for the Masses (2015)

The discovery of induced pluripotent stem cells and the ability to manufacture them using clinically compliant protocols has the potential to revolutionize the field of regenerative medicine. However, realizing this potential requires the development of processes that are reliable, reproducible, and cost-effective and that at the same time do not compromise the safety of the individuals receiving this therapy. In the present report, we discuss how cost reductions can be obtained using our experience with obtaining approval of biologic agents, autologous therapy, and the recent approval of cord blood banks. Significance: For therapy to be widely available, the cost of manufacturing stem cells must be reduced. The steps proposed in the present report, when implemented, have the potential to reduce these costs significantly.