gebhart/patrepeval huggingface dataset:
Determination of the Packing Mode of the Coiled-Coil Domain of cGMP-Dependent Protein Kinase Iα in Solution Using Charge-Predicted Dipolar Couplings (2005)

Coiled-coil motifs are ubiquitous in biology and play essential roles in protein assembly and molecular recognition. Here, we show that the relative orientation and stoichiometry of coiled-coil proteins in solution can be determined by comparison of residual dipolar couplings (RDCs) measured in charged liquid-crystalline medium with values predicted from the three-dimensional charge distribution of the protein. Comparison of charge-predicted RDCs with a small set of one-bond 1DNH dipolar couplings, measured in the negatively charged liquid-crystalline Pf1 bacteriophage medium, identified the coiled-coil region of the cGMP-dependent protein kinase I as a parallel homodimer in solution and ruled out an antiparallel dimeric or monomeric state. The method is very rapid, applicable to a wide variety of liquid crystals used in biological NMR to date, and can be applied to coiled-coil structures and other proteins with higher order assembly.
Nanoelectromechanical systems: A new opportunity for microelectronics (2009)

Transitioning nanoscale devices from the realm of one-of-a-kind feats into robust and reproducible nanosystems - that is, useable technology - is a monumental challenge that transcends the capabilities of any one laboratory. Some solid progress is now being achieved toward this end, and these efforts are critical for realizing the promise of ldquoactiverdquo nanotechnology. At least two essential elements must be in place to realize the vast applications potential that awaits. First, an unfamiliar fusion of technologies is required; one that melds techniques from traditionally-separate disciplines with an appropriate scale of approach. Second, robust methods for large-scale-integration of complex nanodevices are required, and these must engender routes to production en masse. To illustrate the emerging potential of nanosystems I will describe several advances in nanoelectromechanical systems (NEMS) that offer immense promise for applications ranging from metrology, environmental sensing, the life sciences, and health care.
Acute Leukemia in Pregnancy (1978)

ACUTE leukemia is not rare in adults of reproductive age. With the advent of increasingly effective chemotherapeutic regimens, 62% of patients with leukemia from 20 to 34 years of age can be expected to obtain complete remission. The median anticipated length of survival presently exceeds 60 weeks, and we hope this will improve rapidly.<sup>1</sup>Therefore, the management of leukemia during pregnancy will require increasing attention. Management decisions are difficult, and data are sparse. One important consideration is the potential effect of chemotherapy on the fetus. Two women received intensive chemotherapy and achieved remission while carrying pregnancies to term. <h3>Report of Cases</h3><h3>Case 1.—</h3> A 22-year-old woman, gravida 2 and para 1, was admitted to the University Hospital on Sept 14, 1973, with a three-week history of increasing weakness and melena. She was approximately four months pregnant according to history. At the time of admission, she had a temperature of 38.7
Extensive Gene Diversity in Septicemic <i>Escherichia coli</i> Strains (2005)

Extraintestinal pathogenic Escherichia coli strains (ExPEC) are the cause of a diverse spectrum of invasive infections in humans and animals, and these infections often lead to septicemia. Strains of serogroups O2 and O78 of E. coli are involved in human urinary tract infections and newborn meningitis and also constitute the major serotypes involved in avian colisepticemia. In the present study we compared the unique genomic sequences of two such septicemic strains, strains O2-1772 and O78-9, obtained by suppression subtractive hybridization. Evaluation of the degree of similarity between these two strains, which cause the same disease, revealed a high degree of diversity, with only a few shared genes. Subsequently, additional strains of each serogroup of human and animal origin were screened by PCR, and the results provided further evidence for the existence of a high degree of genome plasticity. These results were unexpected, in view of data showing that the two O157:H7 strains that have been sequenced are nearly identical in terms of virulence factors. Furthermore, the data obtained for the septicemic strains suggest that each step in the infection can be mediated by a number of alternative virulence factors, indicating the existence of a mix-and-match combinatorial system. Although whole-genome comparisons of E. coli strains causing different diseases have shown great differences in gene contents, we show that such differences exist even within strains that cause the same disease and that target the same host tissues. Moreover, in addition to the high level of genome plasticity, we show that the large pool of virulence genes in the septicemic strains is independent of the host, implying a high degree of zoonotic risk.
Lenticular Oxygen Toxicity (2003)

High oxygen load has a toxic effect on bovine lenses in organ culture. These effects appear to be cumulative: the higher the oxygen partial pressure and the greater the number of exposures, the more severe the changes observed in the lenses. Changes marking toxicity follow the route of oxygen diffusion into the lens, from the periphery to the center. Cautious interpretation of the results may indicate a role of oxygen (and/or its derivatives) in human cataract formation.
A note on the determination of the ester sulphate content of sulphated polysaccharides (1962)

7-hydroxyl group of flavonols; for the present, it is suggested that a separate enzyme is required for catalysing this step in the synthesis. SUMMARY 1. Nine flavonol glycosides have been found in potato flowers. They are the 3-glucosides, 3rhamnosylglucosides, 3-diglucosides and 3-gluc- osylrhamnosylglucosides ofkampferol and quercetin and the 3-rhamnosylglucoside of myricetin.
Transport mechanisms during the high-temperature oxidation of ternary γ/γ′ Co-base model alloys (2019)

Abstract Over a decade ago, γ ′-strengthened Co-base alloys were introduced as potential replacement for conventional Ni-base Superalloys. Insufficient resistance against high-temperature oxidation restricts the number of possible applications. The present study contributes to the understanding of elementary mechanisms such as material transport during extensive oxide scale formation on γ / γ ′ Co-base alloys to explain their inferior oxidation behaviour. A clear dependency of the scale growth kinetics on W content and oxidation temperature is demonstrated by thermogravimetry and subsequent analysis of cross-sections. By means of electron backscattered diffraction (EBSD), the evolution of microstructures in the outer oxide layers were examined depending on the oxidation temperature. Sequential exposure of samples in 16 O 2 - and 18 O 2 -containing atmospheres proved counter-current material transport. The combination of focused ion beam (FIB) and secondary ion mass spectroscopy (SIMS) visualised the formation of new oxide phases mainly on the outer and inner interface of the oxide scale. An elaborate review of available transport paths for oxygen is given during the discussion of results. All experimental findings were combined to a coherent explanation of the inferior oxidation resistance of this relatively new class of high-temperature materials at temperatures above 800 °C.
Modeling of industrial nylon-6,6 polymerization process in a twin-screw extruder reactor. II. Neural networks and hybrid models (1999)

This article describes the application of neural networks and hybrid models to the finishing stage of nylon-6,6 polycondensation in a twin-screw extruder reactor. A planned experiment in the industrial and in the pilot plant was employed to build the neural network and the hybrid model. The hybrid model combines information calculated from the phenomenological model with the neural network model. The comparison of experimental with calculated data shows good agreement. During two years, industrial data were collected. The comparisons of the models' prediction with these data were performed and reasonable results are achieved from the industrial point of view. These models help an increase of industrial production of about 20%. © 1999 John Wiley & Sons, Inc. J Appl Polym Sci 72: 905–912, 1999
Expression of BNIP3 in invasive breast cancer: correlations with the hypoxic response and clinicopathological features (2009)

BNIP3 expression is lost in a significant portion of invasive breast cancers, which is correlated with poor prognostic features such as positive lymph node status and high proliferation, but not with the hypoxic response.
<title>Shape-based interpolation of gray-scale serial slice images</title> (1993)

A new algorithm for interpolation between grayscale serial slice images is presented. The new algorithm (SBIG) applies shape-based interpolation to CT and MRI images and outputs a dense image volume. Unlike algorithms, such as linear interpolation, which rely only on pixel position, SBIG makes essential use of image content to interpolate between pixels and structure of similar shape and intensity which may differ in size and position from slice to slice. A quantitative comparison shows that SBIG significantly outperforms linear interpolation, particularly as the distance between slices increases. More importantly, while linear interpolation demonstrates characteristic low-pass smearing of object edges and detail, high frequency image features such as object edges and anatomical structures are preserved and well approximated with SBIG. As a result, reconstructed coronal slices from the dense image volume using SBIG demonstrate significantly smoother representation of anatomical structures and less 'staircasing' than those created using linear interpolation.
Nuclear refraction in<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" display="inline"><mml:mrow><mml:mmultiscripts><mml:mrow><mml:mi mathvariant="normal">N</mml:mi></mml:mrow><mml:mprescripts/><mml:mrow/><mml:mrow><mml:mn>14</mml:mn></mml:mrow><mml:mrow/><mml:mrow/></mml:mmultiscripts></mml:mrow></mml:math><mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" display="inline"><mml:mrow><mml:msup><mml:mrow><mml:mo>+</mml:mo></mml:mrow><mml:mrow><mml:mn>12</mml:mn></mml:mrow></mml:msup></mml:mrow></mml:math>C elastic scattering at<i>E</i>/<i>A</i>=20 MeV (1990)

The elastic scattering of 280-MeV $^{14}\mathrm{N}$ off $^{12}\mathrm{C}$ has been measured. The angular distribution, extending up to 38\ifmmode^\circ\else\textdegree\fi{} in the c.m. system, displays a Fraunhofer crossover followed by a structureless falloff, indicative of the influence of nuclear refraction. Results of an optical-model analysis of the data, using phenomenological and folding model potentials, are presented.
ChemInform Abstract: Synthesis and Structure of the First Cyclophosphazene Containing a Metal‐Metal Bond Within the Ring Skeleton. (1988)

Abstract The equimolar reaction of (I) with the hexachloride (II) results in the formation of the known cyclotungstaphosphazene (II) and the W‐W compound (IV).
Does Dirty Harry Have the Answer? (2010)

As crime rates rise, does public support for the rule of law wane? To answer this question, this article examines citizens' reactions to the current crime epidemic in Central America. An analysis of survey data gauges the impact of crime and institutional performance on citizen support for the rule of law. The results indicate that crime has the potential to erode public support for the law, but the linkage is mediated by national context.
Redox-Induced Conversion Pathways in Rhodium and Iridium Complexes Containing C-S Bond Cleaved Benzo[b]thiophene (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTRedox-Induced Conversion Pathways in Rhodium and Iridium Complexes Containing C-S Bond Cleaved Benzo[b]thiopheneClaudio Bianchini, Veronica Herrera, M. Victoria Jimenez, Franco Laschi, Andrea Meli, Roberto Sanchez-Delgado, Francesco Vizza, and Piero ZanelloCite this: Organometallics 1995, 14, 9, 4390–4401Publication Date (Print):September 1, 1995Publication History Published online1 May 2002Published inissue 1 September 1995https://pubs.acs.org/doi/10.1021/om00009a049https://doi.org/10.1021/om00009a049research-articleACS PublicationsRequest reuse permissionsArticle Views115Altmetric-Citations22LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Theoretical DFT Study of Atomic Structure and Spin States of the Co<sub><i>x</i></sub>(C<sub>60</sub>)<sub><i>n</i></sub> (<i>x</i> = 3−8, <i>n</i> = 1,2) Complex Nanoclusters (2008)

Atomic structure and spin states of a set of Cox and Cox(C60)n (x = 3−8, n = 1, 2) clusters are studied using a sophisticated ab initio GGA calculations. It is shown that high-spin low-symmetry structure of free-standing Cox clusters is determined by Jahn−Teller distortions. Formation of η2, η2′ or η1 coordination bonds between Cox fragment and C60 molecules through carbon hexagons results in stable complex nanoclusters with nonmonotonic change of average spin momentum upon the number of cobalt atoms in the Cox cores. The theoretical results are compared with corresponding experimental data.
Five Month Survival in a Calf Supported With an Intraventricular Axial Flow Blood Pump (1995)

We are studying in vivo an intraventricular axial flow blood pump (Jarvik 2000) designed for long-term left ventricular support. The small (25 cc, 85 g) valveless pump has been placed intraventricularly in seven calves; pumps have functioned for as long as 5 months. In the four most recent long-term studies completed, calves have survived for 70, 120, 155, and 162 days (in that order); weight gain has averaged 0.56 kg/day. One study is ongoing at more than 30 days. Under resting physiologic conditions in the normal calf, the continuous flow pump produces flows of 5-6 L/min with a decreased arterial pulse contour. The device has caused no physiologic complications. Calves in the completed studies had mean free plasma hemoglobin levels of 11.4, 7.1, 6.5, and 4.3 mg/dl, respectively. We have modified the inflow structures of the device, and these results suggest that a thrombus free design with no pannus at or around the inlet of the pump can be achieved. Histopathologic analyses of the heart and kidneys in studies of as long as 5 months show no deleterious effects of this device. These studies demonstrate the feasibility of a small implanted intraventricular blood pump for long-term use. Future developments for permanent implantation will include implanted physiologic control systems, transcutaneous energy transmission systems, and implanted batteries.
Structural genes of the mouse major urinary protein are on chromosome 4. (1982)

The major urinary proteins (MUPs) of mouse are a family of at least three major proteins which are synthesized in the liver of all strains of mice. The relative levels of synthesis of these proteins with respect to each other in the presence of testosterone is regulated by the Mup-a locus located on chromosome 4. In an effort to determine the mechanism of this regulation in molecular terms, a cDNA clone containing most of the coding region of a MUP protein has been isolated and identified by partial DNA sequence analysis. Using a combination of hybridization analysis and somatic cell genetics, the structural gene family has been unambiguously mapped to mouse chromosome 4. These data suggest that Mup-a regulation operates in a cis fashion and that models proposing trans regulation of MUP protein synthesis are unlikely.
SOME OBSERVATIONS AND MEASUREMENTS ON STRATOSCOPE I-NEGATIVES (1962)

Analysis of high-definition photographs of sunspots with balloon-borne stratoscope i telescope
The Transcriptional Antiterminator RfaH Represses Biofilm Formation in<i>Escherichia coli</i> (2006)

We investigated the influence of regulatory and pathogenicity island-associated factors (Hha, RpoS, LuxS, EvgA, RfaH, and tRNA5Leu) on biofilm formation by uropathogenic Escherichia coli (UPEC) strain 536. Only inactivation of rfaH, which encodes a transcriptional antiterminator, resulted in increased initial adhesion and biofilm formation by E. coli 536. rfaH inactivation in nonpathogenic E. coli K-12 isolate MG1655 resulted in the same phenotype. Transcriptome analysis of wild-type strain 536 and an rfaH mutant of this strain revealed that deletion of rfaH correlated with increased expression of flu orthologs. flu encodes antigen 43 (Ag43), which mediates autoaggregation and biofilm formation. We confirmed that deletion of rfaH leads to increased levels of flu and flu-like transcripts in E. coli K-12 and UPEC. Supporting the hypothesis that RfaH represses biofilm formation through reduction of the Ag43 level, the increased-biofilm phenotype of E. coli MG1655rfaH was reversed upon inactivation of flu. Deletion of the two flu orthologs, however, did not modify the behavior of mutant 536rfaH. Our results demonstrate that the strong initial adhesion and biofilm formation capacities of strain MG1655rfaH are mediated by both increased steady-state production of Ag43 and likely increased Ag43 presentation due to null rfaH-dependent lipopolysaccharide depletion. Although the roles of rfaH in the biofilm phenotype are different in UPEC strain 536 and K-12 strain MG1655, this study shows that RfaH, in addition to affecting the expression of bacterial virulence factors, also negatively controls expression and surface presentation of Ag43 and possibly another Ag43-independent factor(s) that mediates cell-cell interactions and biofilm formation.
Light-trails: Distributed Optical Grooming for Emerging Data-Center, Cloud Computing, and Enterprise Applications (2011)

Light-trails – generalized lightpaths – are used for provisioning emerging applications leading to the concept of spatial traffic grooming. We investigate the effects of this technology from the perspective of application support and transport.
Chapter 16: PATH: (1989)

Abstract Established in 1972 in Minnesota, the PATH program is relatively unique in being a foster parent membership organization in which foster parents elect a board of directors responsible for establishing agency policies. Seven charcteristics of the program are the central role of foster parents, individually developed treatment, goal orientation, close foster parent-social worker relationship, educational support, network of support relationships, and professional expectations.
The use of inclusion bodies, isolated from Escherichia coli expressing corticotrophin-releasing hormone precursor, to raise specific antibodies against the neuropeptide moiety (1990)

We have expressed human pre-procorticotrophin-releasing hormone (pre-proCRH) as a fusion protein to beta-galactosidase in Escherichia coli. The chimeric fusion protein was found in insoluble bacterial inclusion bodies. The inclusion bodies were isolated, purified and solubilized, and used as imunogens in rabbits to raise antibodies against the neuropeptide moiety. The antibodies generated were characterized by immunoassays and immunocytochemical techniques. The immunoassay results showed that the recombinant pre-proCRH antibodies cross-reacted with the full-length CRH precursor and several cleavage products derived from it, i.e. CRH(1-41) and CRH(36-41). They did not cross-react with the CRH antagonist CRH(9-41). Extracts of stalk median eminence from various species were also studied. The antibodies cross-reacted with extracts from ovine, bovine, human and rat tissues, exhibiting parallel displacement curves to that of synthetic rat/human CRH(1-41) used as standard. They also cross-reacted with a skin extract of the frog, a species known to contain a CRH-related peptide, i.e. sauvagine, in this tissue. The immunocytochemical studies demonstrated that the antibodies generated against recombinant human pre-proCRH labelled neurones in the rat paraventricular nucleus of the hypothalamus. They exhibited the same pattern of staining as that obtained with an antibody generated against synthetic CRH(1-41). The results indicate that these antibodies can recognize CRH(1-41) or CRH-related molecules in the hypothalamus in situ as well as in tissue extracts from several species. Hence, they will be useful tools in the study of the CRH biosynthetic pathway and its intracellular compartmentalization.
Query-by-example - Operations on hierarchical data bases (1899)

Query-by-Example is a high level non-procedural data base language which provides the end user with a simplified unified interface for querying, updating, defining, and maintaining, the data base, as well as imbedding various integrity and authority constraints. When querying the data base the user fills in, through a keyboard display, an example of a possible answer in a skeleton of the logical structure of the data base. As demonstrated in previous work, when the data base is relational, skeleton tables are used. In this paper, we show that the Query-by-Example operations are in fact independent of the structure of the data base. In particular, we demonstrate that if the view at the user interface level is hierarchical, the query is again accomplished by filling-in an example of a possible answer, but, in this case, a skeleton of the hierarchy is utilized. It is, also, shown how the user can map a relational view into a hierarchical view and vice versa, formulating queries that involve both views simultaneously. Finally, a linear version of Query-by-Example is made available for situations where a display facility is unavailable.
Unidirectional degradation of <I>Valonia</I> cellulose microcrystals subjected to cellulase action. (1985)

International audience
Formation of YBa2Cu3O7−δ superconducting films by electrodeposition (1999)

Superconducting thick films of Y-Ba-Cu-O have been prepared via an electrodeposition procedure of the constituent metals on metallic substrates (Cu, Ag) followed by an oxidative thermal treatment. The films were electrodeposited at a constant potential of –3.5 V (vs. Ag/AgCl) from a single electrolyte bath, which contains the nitrates of the cations in dimethyl sulfoxide. The initial state of the precursor film and the formation process of the final superconducting film have been followed by IR spectroscopy, thermogravimetric analysis, X-ray diffraction and scanning electron microscopy. In the precursor film, yttrium and copper are present as metals while barium forms a carbonate. Dimethyl sulfoxide (DMSO) is not inert under the experimental conditions and oxidises to the sulfone. The superconducting transition temperature (T c ) of the final film was ≈92 K and the critical current density (J c ) values were ≈9 500 A cm –2 at 77 K and zero field, the highest so far obtained by electrodeposition.