gebhart/patrepeval huggingface dataset:
Genetic or chemical protease inhibition causes significant changes in the <b><i>Bacillus subtilis</i></b> exoproteome (2008)

Bacillus subtilis is a prolific producer of enzymes and biopharmaceuticals. However, the susceptibility of heterologous proteins to degradation by (extracellular) proteases is a major limitation for use of B. subtilis as a protein cell factory. An increase in protein production levels has previously been achieved by using either protease-deficient strains or addition of protease inhibitors to B. subtilis cultures. Notably, the effects of genetic and chemical inhibition of proteases have thus far not been compared in a systematic way. In the present studies, we therefore compared the exoproteomes of cells in which extracellular proteases were genetically or chemically inactivated. The results show substantial differences in the relative abundance of various extracellular proteins. Furthermore, a comparison of the effects of genetic and/or chemical protease inhibition on the stress response triggered by (over) production of secreted proteins showed that chemical protease inhibition provoked a genuine secretion stress response. From a physiological point of view, this suggests that the deletion of protease genes is a better way to prevent product degradation than the use of protease inhibitors. Importantly however, studies with human interleukin-3 show that chemical protease inhibition can result in improved production of protease-sensitive secreted proteins even in mutant strains lacking eight extracellular proteases.
Effects and Site of Action of Morphine on Gonadotropin Secretion in the Female Rhesus Monkey* (1982)

The effects of morphine on gonadotropin secretion, and the site of its action, were tested in female rhesus monkeys. In Exp 1, morphine sulfate (3, 6, or 9 mg iv) was injected into ovariectomized monkeys, and its effects on tonic (pulsatile) LH and FSH secretion were examined. Administration of morphine (9 mg) resulted in a significant decrease in circulating LH and FSH, which lasted for 4-5 h. Exp 2 was performed to evaluate the site of action of morphine, whether hypophyseal or suprahypophyseal. The effects of morphine (6, 9, or 12 mg) on the LH response to GnRH pulses were evaluated in stalk-sectioned monkeys, in which gonadotropin secretion had been restored by long term pulsatile infusion of GnRH. LH responses to GnRH were not significantly altered by morphine. Exp 3 was performed to determine the effects of morphine on the estrogen-induced LH surge. Estradiol benzoate (330 micrograms in oil) was administered on days 2-5 of the menstrual cycle to nine animals. Four of these also were injected with 9 mg morphine at 5-h intervals for 40 h. Four of the five control and three of the four morphine-treated monkeys showed similar LH surges. The results demonstrate that, in the monkey, opiates inhibit tonic (pulsatile) gonadotropin secretion, most probably by acting at a suprahypophyseal site. In contrast, morphine does not alter the estradiol-induced LH surge, a result that differs from that seen in lower species and that may be related to differences in estradiol positive feedback characteristics.
Distal Tibial Intramedullary Nailing Using an Extraarticular, Lateral Parapatellar Approach in the Semiextended Position (2018)

Semiextended positioning can improve the surgeon's ability to obtain, maintain, and fluoroscopically evaluate a fracture reduction when performing fixation with an intramedullary nail, especially in fractures at the proximal and distal ends. Furthermore, this position allows for evaluation of instrument placement, including the start point, without moving the fluoroscopic unit into extremes of angulation or compromising the quality of the beam orientation. The intraarticular suprapatellar approach has been described as a soft tissue approach to maintain the leg in a position that would not complicate management of these fractures, especially those in the proximal third of the tibia. A semiextended extraarticular soft tissue approach to the start point was described by Kubiak et al, and the lateral parapatellar version has become commonly used on the Orthopaedic Trauma Service at Washington University in Saint Louis. This video demonstrates advantages of semiextended positioning while performing reduction and intramedullary nail fixation for distal tibia fractures. This lateral parapatellar approach can be performed without specialized instrumentation, results in precise establishment of the start point and completion of the fixation without injury to the knee.
Visual stimulation as pain relief for Hong Kong Chinese patients with leg ulcers (2003)

Analgesic potential of visual stimulation was examined in 33 patients with leg ulcers in a randomized, controlled, crossover clinical trial.Patients were alternating between wearing an eyeglass display with soundless VCD broadcast (V-sessions) and a static blank screen (B-sessions) while receiving superficial debridement and wound dressing for their leg ulcers.A significant reduction in pain scores was found during V-sessions (VAS 67.7 ± 28.4 vs. 25.6 ± 29.8 when V-sessions vs. B-sessions, with p < 0.01).Age was positively correlated with the improvement in VAS, whereas gender, residency, and the underlying medical conditions were not correlated with the improvement in pain score.The use of visual stimulation might be beneficial to both genders, in an older age group regardless of the underlying medical conditions.This is the pioneer use of visual stimulation as a non-pharmacological adjuvant to pain relief among a local Chinese population.The study will certainly add knowledge to the existing pain relief methods.
STUDIES OF ACOUSTIC BEHAVIOR OF 3-(2-BENZIMIDAZOL)-3-NITRO-6-METHYL- CHROMEN-4-ONE (BNMC) IN A DIFFERENT SOLVENTS SYSTEM BY USING ULTRASONIC INTERFEROMETER (2014)

By using various acoustic parameters ultrasonic velocity of3-(2-benzimidazol)-3-nitro-6-methyl-chromen-4 one (BNMC) in different solvent mixtures has
Reformulating Component Identification as Document Analysis Problem (2007)

Abstract: One of the first steps of component procurement is the identification of required component features in large repositories of existing components. On the highest level of abstraction, component requirements as well as component descriptions are usually written in natural language. Therefore, we can reformulate component identification as a text analysis problem and apply latent semantic analysis for automatically identifying suit-able existing components in large repositories, based on the descriptions of required component features. In this article, we motivate our choice of this technique for feature identification, describe how it can be applied to feature tracing problems, and discuss the results that we achieved with the application of this technique in a
ChemInform Abstract: SYNTH. VON D,L‐PROSTAGLANDIN‐E(1)‐METHOXIM (1970)

Abstract 2′‐Carboxy‐5‐oxo‐cyclopent‐1‐en‐heptansäure (Ia) wurde zu (Ib) verestert und dann mit N‐Brom‐succinimid zu (IIa) bromiert, aus dem mit Silberacetat über den Ester (IIb) die Hydroxyverbindung (IIc) und ihr Silyläther (IId) hergestellt werden.
Tailoring Strongly Nonlinear Negative Stiffness (2013)

Negative, nonlinear stiffness elements have been recently designed as configurations of pairs or groups of linear springs. We propose a new design of such a system by using a single linear spring with its moving end rolling on a path described by an equation of varying complexity. We examine the effect that the selection of the path has on the size of the deflection regime where negative stiffness is achieved. The stability properties of the equilibrium positions of the system are also investigated, highlighting the influence that the complexity of the path equation brings. The latter naturally affects the characteristics of the forcing functions around these positions. It is demonstrated that the properties of the system can be tailored according to the nature of the equation used and we show how essentially nonlinear negative stiffness elements, (i.e., with no linear parts) can be designed. These results provide a useful standpoint for designers of such systems, who wish to achieve the desired properties in reduced space, which is a common requirement in modern applications.
Spectral Efficiency Maximization for Massive Multiuser MIMO Downlink TDD Systems via Data Power Allocation with MRT Precoding (2017)

This paper studies the problem of maximizing the spectral efficiency in a massive multi-user MIMO downlink (DL) system where the base station employs a large number of antennas and serves single-antenna users in one cell, assuming time- division duplex transmission. In order to maximize the spectral efficiency in a DL transmission, a new method for power allocation among users is proposed based on the water-filling approach. To this end, a lower bound on the achievable rate is derived for each user in the presence of maximum- ratio transmission precoding and employed in defining the spectral efficiency. Since maximizing the spectral efficiency is an NP-hard problem, an effective algorithm is also proposed to find Karush-Kuhn-Tucker (local maximum) points. The performance of the proposed power allocation method is verified by conducting simulations and shown to be superior to other existing methods in terms of higher spectral efficiency.
A very efficient system for alkene epoxidation by hydrogen peroxide: catalysis by manganese-porphyrins in the presence of imidazole (1985)

In the presence of imidazole, Mn-(or Fe-) porphyrins catalyse the epoxidation of various alkenes including alk-1-enes by H2O2; with chloromanganese(tetra-2,6-dichlorophenylporphyrin) as a catalyst, alkene conversion by a few equivalents of H2O2 is complete within less than 1 h at 20 °C and epoxide yields are very high (90–99%).
[Anxiety and depressive disorders in cardiovascular diseases]. (1998)

Cardiovascular diseases are often associated with anxiety and depression. The literature gives different interpretations of this comorbidity. Psychopathological factors have been successively studied as risk factors, comorbid disorders and factors influencing the prognostic. The authors describe the incidence and the prevalence of anxiety and depression, first in coronary heart disease and secondly before cardiac surgery. The prognostic significance of these psychological factors is also reviewed. Physiopathological hypothesis and practical application in clinical practice are proposed.
Photorefractive Keratectomy in a Patient with Epithelial Basement Membrane Dystrophy (2007)

PRK in patients with EBMD could result in favorable refractive and clinical outcome.
Activity-based mission planning and plan management for autonomous vehicles (2002)

This paper presents an activity-based mission planning and plan management framework that has been developed at the Charles Stark Draper Laboratory. The main contributions of this paper are definition of this activity-based implementation and comparison with other planning implementation approaches (e.g., behavior-based); and explanation of how planning, execution, monitoring, and replanning are implemented within this activity-based approach. One of the main benefits of this approach is the ability to separate the mission planning and plan management algorithms from activity specific algorithms and from mission and vehicle specific information. This separation results in an implementation that is highly portable both between missions and vehicles. This framework has been implemented and demonstrated in high-fidelity autonomous land and underwater vehicle simulations, is planned to be implemented on ARPA's UUV in the near future, and is being considered for applications involving other underwater vehicles, autonomous land rovers, and the space shuttle.
Effect of NPY5R Antagonist MK‐0557 on Weight Regain after Very‐low‐calorie Diet‐induced Weight Loss (2007)

Although the difference in weight regain between placebo- and MK-0557-treated patients was statistically significant, the magnitude of the effect was small and not clinically meaningful. Antagonism of the neuropeptide Y Y5 receptor is not an efficacious treatment strategy for reducing weight regain after VLCD.
Hollow-waveguide integrated optics for 10.6-μm lidar (1998)

A novel integrated optic approach to the manufacture of 10.6 micrometers coherent laser radar systems is described and demonstrated. The approach uses hollow waveguides to guide light between system components which are integrated into a common substrate. The design, manufacture and operation of a seven element laser radar subsystem which is compact, rugged and provides a mixing efficiency in excess of 80% of the theoretical maximum, is discussed. Experiments which confirm the validity of the design criteria upon which the system is based are described. The demonstration of the subsystem as a simple homodyne vibrometer is also outlined.
The Novel GLP-1-Gastrin Dual Agonist ZP3022 Improves Glycemic Control in ZDF Rats (2012)

OGTT. A) BG profile during the OGTT, and B) AUC. Data were analyzed using one-way ANOVA followed by Dunnett's multiple comparison test, *p < 0.05, ***p < 0.001 vs. vehicle; or Mann Whitney test, ### p < 0.001 vs. vehicle, ¤p < 0.05 vs. liraglutide. n = 9-12. Data are mean ± SEM.
MLCT-dd energy gap in pyridyl-pyrimidine and bis(pyridine) complexes of ruthenium(II) (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMLCT-dd energy gap in pyridyl-pyrimidine and bis(pyridine) complexes of ruthenium(II)D. Paul Rillema, Charles B. Blanton, Randy J. Shaver, Donald C. Jackman, Massoud Boldaji, Stephanie Bundy, Laura A. Worl, and Thomas J. MeyerCite this: Inorg. Chem. 1992, 31, 9, 1600–1606Publication Date (Print):April 1, 1992Publication History Published online1 May 2002Published inissue 1 April 1992https://pubs.acs.org/doi/10.1021/ic00035a016https://doi.org/10.1021/ic00035a016research-articleACS PublicationsRequest reuse permissionsArticle Views852Altmetric-Citations99LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Thalidomide Metabolism and Hydrolysis: Mechanisms and Implications (2006)

Despite its controversial past, thalidomide is currently under investigation for the treatment of several disease types, ranging from inflammatory conditions to cancer. The mechanism of action of thalidomide is complex and not yet fully understood, but there is some evidence to suggest that metabolism may play a role. Consequently, there has been a considerable effort to characterize the metabolism of thalidomide in recent years. Thalidomide undergoes biotransformation by non-enzymatic hydrolysis and enzyme-mediated hydroxylation to form a multitude of metabolites. Metabolite identification and reaction phenotyping studies have been performed and will be discussed in this review in addition to interspecies differences in thalidomide metabolism.
[Effectiveness of expression of the chloramphenicol acetyltransferase gene controlled by foreign regulatory regions in Escherichia coli cells. I. Construction of vectors for the cloning of transcription regulatory elements]. (1986)

New plasmids pML2.1 and pML4 were constructed for cloning the transcription regulatory regions. In the pML2.1 the structural part of chloramphenicol acetyltransferase gene of the pBR325 is under control of the lacUV5-promotor. Because the unique BamH1 cleavage site is in the joint region, one may use it for cloning transcription termination regions and selecting recombinant clones with the AprCms phenotype. As for the pML4, the foreign fragment integration is carried directly before the structural part of cat-gene and it is expressed only if the promotor regions are present. The plasmids were sequenced and their restriction maps were established. Small molecular weight (about 2,0 MDa, AprCmr) or only Apr intact genes and convenient disposition of many unique cleavage sites by restriction endonucleases make these plasmids useful for different genetic engineering experiments.
Three Faces of Human-Computer Interaction (2005)

Human-computer interaction is considered a core element of computer science. Yet it has not coalesced; many researchers who identify their focus as human-computer interaction reside in other fields. The author examines the origins and evolution of three HCI research foci: computer operation, information systems management, and discretionary use. The author describes efforts to find common ground and forces that have kept them apart.
The effect of the oxytocin antagonists, CAP 476 and F327, on calcium mobilisation in single cultured human myometrial cells (1993)

ABSTRACT Objective To investigate the mechanism of action of the oxytocin (OT) antagonists, CAP 476 and F327. Design A prospective descriptional study. Subjects Women undergoing caesarean section at term or hysterectomy. Interventions Myometrial cells were cultured from uterine biopsies. Main outcome measures Intracellular calcium ([Ca 2+ ] i ), determined in single cells. Results Application of OT caused a transient increase in [Ca 2+ ] i . CAP 476 abolished and F327 reduced the response to OT but neither reduced the [Ca 2+ ] i transient induced by cell depolarisation with 120 mmol K + . CAP 476 did not reduce transients caused by prostaglandin E 2 . F327 reduced the frequency of repetitive [Ca 2+ ] i transients occurring during continuous application of OT. Conclusions The results demonstrate that the antagonists reduce the effect of OT and that their action is relatively specific. Their mechanism of action as clinical tocolytic agents is discussed.
Investigation of switching effects between the drains of an electron Y-branch switch (2001)

By using high-resolution electron beam lithography and wet etching, Y-branched electron waveguides with lengths down to 70 nm have been fabricated on modulation-doped GaAs/AlGaAs heterostructures. Small positive bias applied between the source and the two drain electron reservoirs leads to enhanced switching of electrons into either of the two branches when a lateral external electric field is applied. The switching manifests itself by pronounced sawtooth oscillations in the conductance between source and one branch of an electron Y-branch switch as the gate voltage at the other branch is changed when the corresponding gate voltage is fixed.
Sympathetic Activity-Mediated Neuropathic Facial Pain Following Simple Tooth Extraction: A Case Report (2002)

This is a report of a case of sympathetic activity-mediated neuropathic facial pain induced by a traumatic trigeminal nerve injury and by varicella zoster virus infection, following a simple tooth extraction. The patient had undergone extraction of the right lower third molar at a local dental clinic, and soon after the tooth extraction, she became aware of spontaneous pain in the right ear, right temporal region, and in the tooth socket. At our initial examination 30 days after the tooth extraction, the healing of the tooth socket was normal; however, the patient had a tingling and burning sensation (dysesthesia) and spontaneous pain of the right lower lip and the right temporal region, both of which were exacerbated by non-noxious stimuli (allodynia). The patient also showed paralysis of the marginal mandibular branch of the facial nerve, taste dysfunction, and increased varicella zoster serum titers. A diagnostic stellate ganglion block (SGB) 45 days after the tooth extraction using one percent lidocaine markedly alleviated the dysesthesia and allodynia. These symptoms are characteristic of neuropathic pain with sympathetic interaction. The patient was successfully treated with SGB and a tricyclic antidepressant.
A NEW FLASH ARCHITECTURE WITH A 5.83L2 SCALABLE AMG FLASH CELL (1997)

We have introduced a new flash array architecture called AMG aternate Metal virtual Ground) to address the high density and low voltage (3V only) applications. A 2.lpm2 cell size is demonstrated with 0.6pm design rules. A low current programming technique has been developed to enable low voltage operation. A segmented array architecture is introduced t,o eliminat,e cycling induced program-erase disturbs. An innovative planarized array DPCC (Double Poly Contact Circuit) process is developed to reahze the AMG architecture.
Human eosinophils have prolonged survival, enhanced functional properties, and become hypodense when exposed to human interleukin 3. (1988)

Human eosinophils were cultured in the presence of recombinant human IL-3 for up to 14 d and their biochemical, functional, and density properties were assessed. After 3 d of culture in 10 pM IL-3, eosinophils had a viability of 70% compared with only 10% in enriched medium alone. Neither IL-1 alpha, IL-2, IL-4, tumor necrosis factor, basic fibroblast growth factor, nor platelet-derived growth factor maintained eosinophil viability. The 7- and 14-d survival of the cultured eosinophils was 55 and 53%, respectively. No other cell type, including neutrophils, was present after culture. After 7 d of culture, the normodense eosinophils were converted to hypodense cells as assessed by density centrifugation. Eosinophils exposed to 1,000 pM IL-3 for 30 min or cultured in 10 pM IL-3 for 7 d generated approximately threefold more leukotriene C4 (LTC4) in response to calcium ionophore than freshly isolated cells. Furthermore, whereas freshly isolated eosinophils killed only 14% of the antibody-coated Schistosoma mansoni larvae, these eosinophils killed 54% of the larvae when exposed to 100 pM IL-3. The enhanced helminth cytotoxicity was maintained for 7 d when eosinophils were cultured in the presence of both 10 pM IL-3 and 3T3 fibroblasts, but not when eosinophils were cultured in the presence of IL-3 alone. IL-3 thus maintains the viability of eosinophils in vitro, augments the calcium ionophore-induced generation of LTC4, enhances cytotoxicity against antibody-sensitized helminths, and induces the eosinophils to become hypodense cells. These phenotypic changes in the eosinophil may be advantageous to host defense against helminthic infections but may be disadvantageous in conditions such as allergic disease.
Effect of Deuterium Oxide on Growth of HeLa, L and L-5178Y Cells. (1960)

SummaryThe effect of D2O on growth of 3 stable mammalian cell lines (HeLa, L and L-5178Y) has been investigated. As D2O concentration was increased all cells showed increased water content and dry weight and a slower growth rate. Cytological investigations showed an increase in number of multinucleated cells and a moderate increase in sudanophilic material. No significant changes in nucleic acid content were demonstrable with the staining technics used.
ChemInform Abstract: SAPONIFICATION OF DIMETHYL CIS‐2,6‐DIPHENYL‐4‐OXOCYCLOHEXANE‐1,1‐DICARBOXYLATE. A REINVESTIGATION (1982)

Abstract Eine neue Untersuchung ergibt, daß bei der alkalischen Hydrolyse des Dicarbonsäureesters (Ia) eine Spaltung zum ungesättigten Keton (IIa) und dessen Folgeprodukten (IIb)‐(IId) erfolgt.