gebhart/patrepeval huggingface dataset:
A coumarin‐specific prenyltransferase catalyzes the crucial biosynthetic reaction for furanocoumarin formation in parsley (2013)

Furanocoumarins constitute a sub-family of coumarin compounds with important defense properties against pathogens and insects, as well as allelopathic functions in plants. Furanocoumarins are divided into two sub-groups according to the alignment of the furan ring with the lactone structure: linear psoralen and angular angelicin derivatives. Determination of furanocoumarin type is based on the prenylation position of the common precursor of all furanocoumarins, umbelliferone, at C6 or C8, which gives rise to the psoralen or angelicin derivatives, respectively. Here, we identified a membrane-bound prenyltransferase PcPT from parsley (Petroselinum crispum), and characterized the properties of the gene product. PcPT expression in various parsley tissues is increased by UV irradiation, with a concomitant increase in furanocoumarin production. This enzyme has strict substrate specificity towards umbelliferone and dimethylallyl diphosphate, and a strong preference for the C6 position of the prenylated product (demethylsuberosin), leading to linear furanocoumarins. The C8-prenylated derivative (osthenol) is also formed, but to a much lesser extent. The PcPT protein is targeted to the plastids in planta. Introduction of this PcPT into the coumarin-producing plant Ruta graveolens showed increased consumption of endogenous umbelliferone. Expression of PcPT and a 4-coumaroyl CoA 2'-hydroxylase gene in Nicotiana benthamiana, which does not produce furanocoumarins, resulted in formation of demethylsuberosin, indicating that furanocoumarin production may be reconstructed by a metabolic engineering approach. The results demonstrate that a single prenyltransferase, such as PcPT, opens the pathway to linear furanocoumarins in parsley, but may also catalyze the synthesis of osthenol, the first intermediate committed to the angular furanocoumarin pathway, in other plants.
STUDY OF DYNAMICS OF MICROSTRUCTURAL TRANSFORMATIONS IN CRYSTALLINE YTTRIA NANOPOWDER (2013)

PACS 61.05.CThis paper is a continuation of a previously reported study of the microstructure transformations in crystalline yttria nanopowder prepared by soft chemistry via precipitation from aqueous acidic nitrates. We have observed the aforementioned transformations over extended periods of time at 900 C and 1100 C (isothermal annealing) with the use of X-ray diffraction analysis (full profile analysis of reflections, etc.).
Two quantitative trait loci, Dw1 and Dw2, are primarily responsible for rootstock-induced dwarfing in apple (2015)

The apple dwarfing rootstock 'Malling9' ('M9') has been used worldwide both to reduce scion vigour and as a genetic source for breeding new rootstocks. Progeny of 'M9' segregate for rootstock-induced dwarfing of the scion, indicating that this trait is controlled by one or more genetic factors. A quantitative trait locus (QTL) analysis of a rootstock population derived from the cross between 'M9' × 'Robusta5' (non-dwarfing) and grafted with 'Braeburn' scions identified a major QTL (Dw1) on linkage group (LG) 5, which exhibits a significant influence on dwarfing of the scion. A smaller-effect QTL affecting dwarfing (Dw2) was identified on LG11, and four minor-effect QTLs were found on LG6, LG9, LG10 and LG12. Phenotypic analysis indicates that the combination of Dw1 and Dw2 has the strongest influence on rootstock-induced dwarfing, and that Dw1 has a stronger effect than Dw2. Genetic markers linked to Dw1 and Dw2 were screened over 41 rootstock accessions that confer a range of effects on scion growth. The majority of the dwarfing and semi-dwarfing rootstock accessions screened carried marker alleles linked to Dw1 and Dw2. This suggests that most apple dwarfing rootstocks have been derived from the same genetic source.
Einfache Elektrosynthese von 1,2,4‐Triazolin‐3,5‐dionen (1981)

Die in‐situ‐Erzeugung der extrem reaktiven 1,2,4‐Triazolin‐3,5‐dione (1) gelingt vorteilhaft durch Elektrosynthese aus den entsprechenden gesättigten Heterocyclen. Auch die bisher nur unvollständig charakterisierte Stammverbindung (1) , R = H ist auf diese Weise zugänglich. magnified image
Dynamics of Superparamagnetic and Ferromagnetic Nano-Objects in Continuous-Flow Microfluidic Devices (2007)

We present a numerical study on the dynamics of magnetic micro- and nano-objects subjected to magnetic field gradients in conventional continuous-flow microfluidic devices. By a mixed finite-element/discrete-element approach we solve the equations of the field driven motion for magnetic nano-objects floating in liquids at very low Reynolds numbers and compare the magnetic trapping efficiency of commercially available superparamagnetic microbeads to that of ferromagnetic nanowires. The drag force and the remanent magnetization of ferromagnetic nanowires are found to be responsible for the huge increase of their magnetic trappability whereas the slip-length associated with the Navier boundary condition at the transition to the nanoscale regime is found to be a much less important parameter
Laboratory and alt-evaluation of high stiffness underlayers with high percentage of re-use as developed in the nr2c-project (2008)

New Road Construction Concepts (NR2C) is a project of FEHRL supported by the European Commission under the Sixth Framework Programme. This project aims at developing perspectives and specific innovations in different transport areas. One of the ideas concerns the development of high performance underlayers with high percentage of reclaimed asphalt. Three different mixes were designed, optimized and compared, namely with 0 %, 25 % and 40 % reclaimed asphalt. After an extensive laboratory study in BRRC, the selected mixes have been further studied in a full-scale ALT facility in LAVOC. During these tests, resistance to fatigue at 15 Â°C and also low temperature behavior with cycles between 2 Â°C and -7 Â°C have been investigated by applying 12 tons axle loads. In addition to the ALT, wheel tracking tests, tests on cores as well as on big slabs with a controlled temperature gradient have been performed on material taken from the ALT tracks. Moreover, a specific binder analysis has been carried out in order to analyse the evolution of the binder and mix properties. The most important conclusion from this work is that no negative effect, due to the use of a high percentage of reclaimed asphalt, has been observed in this study. However, key parameters such as the RA properties and mix design as function of the RA properties require special attention.
Quantum cascade laser sensor for SO_2 and SO_3 for application to combustor exhaust streams (2005)

We have demonstrated a high-sensitivity, room-temperature quantum-cascade (QC) laser sensor for detection of SO2 and SO3 under conditions relevant to aircraft test combustor exhaust. Two QC lasers probe infrared absorption features at 7.50 and 7.16 microm for SO2 and SO3, respectively, with a common dual-beam detection system. We inferred a noise-equivalent absorbance of approximately 1 x 10(-4) Hz(-1/2). We have demonstrated detection limits for both SO2 and SO3 of 1-2 ppmv m/Hz(1/2) (where ppmv is parts in 10(6) by volume) for 300 torr, elevated temperature, and path lengths near 1 m. This level of sensitivity permits measurement of < 1 ppmv of SO2 and SO3 at these conditions with modest signal averaging.
Comparing Astrocytic Cell Lines that Are Inhibitory or Permissive for Axon Growth: the Major Axon-Inhibitory Proteoglycan Is NG2 (1999)

Astrocytes, oligodendrocytes, and oligodendrocyte/type 2 astrocyte progenitors (O2A cells) can all produce molecules that inhibit axon regeneration. We have shown previously that inhibition of axon growth by astrocytes involves proteoglycans. To identify inhibitory mechanisms, we created astrocyte cell lines that are permissive or nonpermissive and showed that nonpermissive cells produce inhibitory chondroitin sulfate proteoglycans (CS-PGs). We have now tested these cell lines for the production and inhibitory function of known large CS-PGs. The most inhibitory line, Neu7, produces three CS-PGs in much greater amounts than the other cell lines: NG2, versican, and the CS-56 antigen. The contribution of NG2 to inhibition by the cells was tested using a function-blocking antibody. This allowed increased growth of dorsal root ganglion (DRG) axons over Neu7 cells and matrix and greatly increased the proportion of cortical axons able to cross from permissive A7 cells onto inhibitory Neu7 cells; CS-56 antibody had a similar effect. Inhibitory fractions of conditioned medium contained NG2 coupled to CS glycosaminoglycan chains, whereas noninhibitory fractions contained NG2 without CS chains. Enzyme preparations that facilitated axon growth in Neu7 cultures were shown to either degrade the NG2 core protein or remove CS chains. Versican is present as patches on Neu7 monolayers, but DRG axons do not avoid these patches. Therefore, NG2 appears to be the major axon-inhibitory factor made by Neu7 astrocytes. In the CNS, NG2 is expressed by O2A cells, which react rapidly after injury to produce a dense NG2-rich network, and by some reactive astrocytes. Our results suggest that NG2 may be a major obstacle to axon regeneration.
The Colorimetric Detection and Determination of Palladium with Compounds Containing the p-Nitrosophenylamino Group<sup>1</sup> (1941)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe Colorimetric Detection and Determination of Palladium with Compounds Containing the p-Nitrosophenylamino Group1Lyle G. Overholser and John H. YoeCite this: J. Am. Chem. Soc. 1941, 63, 11, 3224–3229Publication Date (Print):November 1, 1941Publication History Published online1 May 2002Published inissue 1 November 1941https://pubs.acs.org/doi/10.1021/ja01856a090https://doi.org/10.1021/ja01856a090research-articleACS PublicationsRequest reuse permissionsArticle Views123Altmetric-Citations41LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
The homeobox gene BARX2 can modulate cisplatin sensitivity in human epithelial ovarian cancer (2002)

Epithelial ovarian cancer (EOC) is the most common cause of death from gynaecological malignancy. Resistance to platinum chemotherapy is a major reason for treatment failure and poor prognosis. The human homeobox gene BARX2 is located within a minimal region at 11q25 that is associated with frequent loss of heterozygosity (LOH) and adverse survival in EOC. BARX2 is a transcription factor known to regulate transcription of specific cell adhesion molecules in the mouse. We have previously shown that BARX2 expression is low in clear cell/endometrioid and high in serous adenocarcinomas of the ovary, histologic variants that are less and more sensitive, respectively to platinum chemotherapy. The aim of this study was to define whether BARX2 could modulate sensitivity to cisplatin in human epithelial ovarian cancer. In two cell line series sequentially derived from ovarian cancer patients pre- and post-cisplatin chemotherapy, BARX2 expression was downregulated in the cell lines established upon tumor recurrence after platinum therapy. Transfection of BARX2 into a platinum resistant cell line significantly reversed cisplatin resistance compared with its isogenic platinum sensitive parent, in both growth inhibition and clonogenic assays. Taken together, our data demonstrate that the homeobox gene BARX2 may be a biological factor involved in determining sensitivity or resistance to the cytotoxic effects of cisplatin.
Pathogenic Yersinia DNA Is Detected in Bowel and Mesenteric Lymph Nodes From Patients With Crohn's Disease (2003)

Previously, we detected pathogenic (invasive) DNA in the appendices of two patients who later developed Crohn's disease (CD). This subsequent investigation is the first to evaluate a series of specimens from CD patients for the presence of pathogenic DNA. A total of 54 intestinal resection specimens from 52 patients with confirmed CD were evaluated. Lesional tissue was tested by polymerase chain reaction analysis for the presence of genes occurring only in pathogenic Primer pairs are specific for each species, with no known cross reactions with other bacteria. Forty normal bowel specimens, 30 cases of acute appendicitis, and 50 cases of various active colitides served as disease controls. Medical records were reviewed following polymerase chain reaction and histologic evaluation. A total of 17 of 54 resections (31%) contained DNA by polymerase chain reaction. Mesenteric lymph nodes were also positive in eight of these cases. All controls were negative. -positive patients had carried the diagnosis of CD for a median of 10 years before resection (range 1 month to 40 years). We report the first documentation of DNA in a series of CD cases. Further studies are needed, including serial study, over time, of -positive CD patients, as well as prospective studies of newly diagnosed CD patients for evidence of infection. Like previous studies associating infectious organisms with CD, much work remains to elucidate whether the presence of DNA is an epiphenomenon or actually a factor in the pathogenesis of CD.
Inversion-free discrimination of unexploded ordnance in real time (2012)

ESTCP live-site UXO classification results are presented for cued data collected with two advanced EMI instruments, the cart-based 2 × 2 3D TEMTADS array and the Man Portable Vector (MPV) handheld sensor, at the former Camp Beale in California. There were two sets of targets of interest (TOI): the main set consisted of 105-mm, 81-mm, 60-mm, 37-mm and ISO projectiles, and the other (optional) set comprised site-specific fuzes and fuze fragments of varous sizes. The advanced models used for inversion and classification combine: 1) a joint-diagonalization (JD) algorithm that estimates the number of potential targets generating an anomaly directly from the measured data without need for inversion; 2) the ortho-normalized volume magnetic source (ONVMS) model, which locates targets, represents their EMI responses, and extracts their intrinsic feature vectors; and 3) a Gaussian mixture algorithm that uses extracted discrimination features to classify the corresponding buried objects as TOI or clutter. Initially the data are inverted using a combination of ONVMS and the differential evolution direct-search algorithm; this allows the determination of relevant intrinsic parameters, which in turn are classified by a mixture of clustering and library-matching techniques. This paper describes in more detail the main steps of the classification process and demonstrates the results obtained for the 2 × 2 3D TEMTADS and MPV data taken at Camp Beale, as scored independently by the Institute for Defense Analyses. The advanced models are seen to produce superb classification in both cases.
Lagrangun-relaxation based mesh traffic grooming for network profit optimization (2005)

The optimization of grooming static traffic is formulated in this paper as a constrained integer linear programming problem and the objective is to minimize the summation of the routing cost and the rejection penalty of the traffic demands. The routing cost takes into account both the cost in the electronic and the optical domains. Such optimization objective represents the network profit optimization and is of practical significance in network planning. Through the optimization process, traffic demands are selectively accepted based on the profit they generate, which is the difference between the revenue and the network cost. We propose a decomposition approach using Lagrangian-relaxation, which can also obtain the theoretical bounds for traffic grooming problem. The comparison with some previous research shows the great advantage of our solution framework. The optimization results for some network examples also indicate that the proposed algorithm achieves good near-optimal solutions.
Cryptosporidium spp. and Giardia duodenalis: A picture in Portugal (2012)

Cryptosporidium is an entero-pathogen which causes gastrointestinal disturbs. Primarily this organism infects the microvillous border of the intestinal epithelium, and to lesser extent extra intestinal epithelia, causing acute gastrointestinal disturbs (Fayer, 2004). The duration of infection and the ultimate outcome of intestinal cryptosporidiosis greatly depend on the immune status of the patient. In fact, immunologically healthy patients usually recover spontaneously in a week. The clinical signs can range from asymptomatic to acute, severe and persistent diarrhea and their potential for Cryptosporidium transmission can persist for weeks after symptoms cease (Deng et al., 2004; Fayer, 2004; Hunter and Thompson, 2005).
Functional group effects on metal–organic framework topology (2012)

Functionalization of the ligand 1,3,5-tris(4-carboxyphenyl)benzene (H(3)BTB) has been realized with methoxy (H(3)BTB-[OMe](3)) and hydroxy (H(3)BTB-[OH](3)) groups. Combining H(3)BTB-[OMe](3) and Zn(II) results in the formation of the first isostructural, functionalized analogue of MOF-177 (MOF-177-OMe), while the combination of H(3)BTB-[OH](3) and Zn(II) generates a rare, interpenetrated pcu-e framework.
Comparison of Endoscopy-Based and Serum-Based Methods for the Diagnosis of<i>Helicobacter pylori</i> (2003)

Testing for serological IgG antibodies to H pylori WC was the best to assess whether infection by H pylori was present. Neither the IgA WC nor the IgG CagA ELISAs add significant value in the diagnosis of H pylori.
Diastereoselection of chiral acids in a cylindrical capsule (2005)

The cylindrical dimeric capsule binds two chiral carboxylic acids with modest selectivity for homochiral or heterochiral pairs.
ChemInform Abstract: A Reinvestigation of the Synthesis of 1,2‐Dihydro[1,2]diazepin‐3‐ones from Pyrones. (1986)

Abstract Reaktion des gemäß Schema hergestellten Hydroxy‐pyranons (V) mit Hydrazinhydrat liefert nicht wie früher beschrieben das Diazepinon (XIV), sondern das Hydroxy‐dihydropyridinon (VI), das zum N‐Aminopyridon (VII) dehydratisiert wird.
IMPELLER RPM DYNAMIC SUSPENSION OF THE BAYLOR GYRO PERMANENTLY IMPLANTABLE CENTRIFUGAL BLOOD PUMP DURING PULSATILE CIRCULATION (2002)

Background: The Baylor Gyro permanently implantable centrifugal pump (PI 710 pump) achieved 90 day survival in an animal experiment as a biventricular assist device without thrombus formation. The aim of this study is to confirm the high antithrombogenicity of PI 710 from the viewpoint of impeller movement. Materials and Methods: The mock circulation loop consisted of an aortic and left atrial chamber, pulsatile pump and PI 710. This circuit was filled with 37% glycerin/water. The pulsatile pump simulated the natural heart while the PI 710 was the left ventricular assist device with left ventricular drainage. An acrylic pump was used in order to observe the impeller. The RPM was changed from 1200 to 2600 rpm, and the impeller movement was examined. Results: The impeller floated up and down synchronized to the pulsation from about 1600 to 1800 rpm (impeller was up during diastole and down during systole). And over 1800 rpm, the impeller contacted the top hearing. In these two conditions, the bottom bearing of the impeller moved to the ouflow side during systole and to the counterside during diastole. Conclusion: The PI 710 impeller was dynamically suspended during pulsatile circulation, and was dependent on RPM. These authors anticipated that these movements have a dynamic effect for antithrombogenicity.
Simian virus 40 T antigen can transcriptionally activate and mediate viral DNA replication in cells which lack the retinoblastoma susceptibility gene product (1990)

Simian virus 40 T antigen is a multifunctional protein which has recently been shown to form a complex with the retinoblastoma susceptibility gene product (Rb protein) (J.A. DeCaprio, J.W. Ludlow, J. Figge, J.-Y. Shaw, C.-M. Huang, W.-H. Lee, E. Marsilio, E. Paucha, and D.M. Livingston, Cell 54:275-283, 1988; P. Whyte, K.J. Buchkovich, J.M. Horowitz, S.H. Friend, M. Raybuck, R.A. Weinberg, and E. Harlow, Nature (London) 334:124-129, 1988). This interaction may facilitate some of the functions of T antigen. The ability of simian virus 40 T antigen to mediate transcriptional activation and viral DNA replication was tested in human osteosarcoma cell lines U-2OS and Saos-2, which are Rb positive and Rb negative, respectively. Both functions of T antigen were efficient in both cell lines. Hence, these functions can occur in the absence of Rb protein.
Physical state of inhibitor fatty acids and linoleate solutions under lipoxygenase assay conditions (1984)

Abstract A variety of fatty acids, which are potential competitive inhibitors of soybean lipoxygenase (EC 1.13.11.12), give kinetically unstable mixtures in standard assay solutions containing linoleate (substrate). In the assay solution (pH 10, 0.1 M borate, 1.63% ethanol), each fatty acid by itself shows normal surface tension vs concentration behavior; but, despite a range of solubilization techniques in the presence of 10 μM or higher linoleate and low concentrations of these materials, irreproducible surface‐tension readings and inhibition kinetics results. This inhomogeneity (or kinetic instability) disappears as the concentration increases. Critical micelle concentration (CMC) values of mixtures are not additive, and binary mixture behavior depends on fatty acid structure. Several lines of observation, including CMC values and actual surface tension (γ) values for several systems, suggest premicellar heterodimer or higher mixed aggregate formation. Lipids with K i significantly above the irreproducible surface‐tension range give good kinetic behavior, and K i is reported. The results are in accord with earlier work on aspects of these systems. Complementary solution physical studies must be done for any kinetic (or specificity) determinations of enzymes using lipids.
Involvement of Holliday Junction Resolvase in Fluoroquinolone-Mediated Killing of Mycobacterium smegmatis (2014)

The absence of the Holliday-junction Ruv resolvase of Mycobacterium smegmatis increased the bacteriostatic and bactericidal activities of the fluoroquinolone moxifloxacin, an important antituberculosis agent. The treatment of ruvAB-deficient cells with thiourea and 2,2'-bipyridyl lowered moxifloxacin lethality to wild-type levels, indicating that the absence of ruvAB stimulates a lethal pathway involving reactive oxygen species. A hexapeptide that traps the Holliday junction substrate of RuvAB potentiated moxifloxacin-mediated lethality, supporting the development of small-molecule enhancers for moxifloxacin activity against mycobacteria.