gebhart/patrepeval huggingface dataset:
UPTAKE OF 3‐<i>O</i>‐METHYL‐<scp>d</scp>‐GLUCOSE INTO CULTURED HUMAN GLIOMA CELLS (1975)

Abstract— Human glioma cells (138 MG) were found to take up 3‐ O ‐methyl‐ d ‐glucose (3‐OMG) by a saturable low affinity transport system with a K m of 20 m m and a V max of 500 nmol/mg protein/min. About 20 per cent of the total uptake was due to passive diffusion. d ‐Glucose was a competitive inhibitor with a K i of 10 m m . Follow‐up experiments indicated that the same transport mechanism is involved in the uptake of n‐glucose and 3‐OMG. Phloretin (0·02 m m ) and cytochalasin B (0·002 m m ) strongly inhibited the uptake of 3‐OMG, whereas phlorizin (0·02 m m ), ouabain (0·1 m m ), NaCN (0·5 m m ) and iodoacetic acid (1·0 m m ) had no effect. The data suggest that 3‐OMG and d ‐glucose enter 138 MG cells mainly by a Na + ‐independent passive carrier‐mediated transport system. Serum‐deprivation doubled the population doubling time (T d ) without affecting the total uptake of 3‐OMG. An increase in the non‐specific (diffusional) uptake was balanced by a decrease in the specific (carrier‐medíated) uptake. After addition of dibutyryl cyclic AMP (dbcAMP, 0·25 m m ) the cells attained a morphology characteristic of differentiated glia cells. T d was maintained unchanged. The non‐specific uptake of 3‐OMG was not affected in cells grown in serum‐containing medium plus dbcAMP, whereas the specific uptake increased by 40 per cent and there‐fore also the total uptake. Similar, but more pronounced, changes were observed if serum‐deprived cells were treated with dbcAMP.
Improvement of encapsulation method of Hibernate for Web Application (2007)

This article has analyzed the Hibernate’s encapsulating method of model classes’s insufficiency in the web application: When the page transmits the value of “”, Hibernate is unable to use mold classes with the view layer together.In view of the com- monly used web application data type, we improved the Hibernate’s encapsulating method of model classes for the web application, and has carried on the confirmation in the Oracle and in the Sql Server 2000 database.
The Genome and Methylome of a Beetle with Complex Social Behavior, <i>Nicrophorus vespilloides</i> (Coleoptera: Silphidae) (2015)

Testing for conserved and novel mechanisms underlying phenotypic evolution requires a diversity of genomes available for comparison spanning multiple independent lineages. For example, complex social behavior in insects has been investigated primarily with eusocial lineages, nearly all of which are Hymenoptera. If conserved genomic influences on sociality do exist, we need data from a wider range of taxa that also vary in their levels of sociality. Here, we present the assembled and annotated genome of the subsocial beetle Nicrophorus vespilloides, a species long used to investigate evolutionary questions of complex social behavior. We used this genome to address two questions. First, do aspects of life history, such as using a carcass to breed, predict overlap in gene models more strongly than phylogeny? We found that the overlap in gene models was similar between N. vespilloides and all other insect groups regardless of life history. Second, like other insects with highly developed social behavior but unlike other beetles, does N. vespilloides have DNA methylation? We found strong evidence for an active DNA methylation system. The distribution of methylation was similar to other insects with exons having the most methylated CpGs. Methylation status appears highly conserved; 85% of the methylated genes in N. vespilloides are also methylated in the hymentopteran Nasonia vitripennis. The addition of this genome adds a coleopteran resource to answer questions about the evolution and mechanistic basis of sociality and to address questions about the potential role of methylation in social behavior.
Removal and Recovery of Milligramme Quantities of HLA Antibodies from Human Sera Using HLA-DR11 Protein Affinity Chromatography (2012)

Introduction: Strong humoral responses to allogeneic Human Leukocyte Antigens (HLA) preclude organ transplantation, promote hyperacute rejection, and contribute to chronic transplant rejection. Therapies exist to prevent B cells from secreting antibodies, and bulk antibody removal is also an option, but there is no means to specifically deplete antibodies that recognize an allogeneic HLA molecule while leaving humoral immunity largely intact. Methods: We produced 70 milligrams of the soluble class II molecule HLA-DRB1*11:01/DRA1*01:01 in mammalian cells, covalently couple 65 mg of purified DR11 to a solid support, and generate a column for the purpose of removing anti-DR11antibodies in liter volumes of patient plasma containing anti-DR11 antibodies. Results: When patient plasma recognizing multiple HLA was passed over the DR11 matrix, antibodies specific for DR11were removed while antibodies for other class II (DQ) specific antibodies passed through intact. The bound DR11specific antibodies were then recovered from the columns with an average quantity of 16.3mg. We then go on to show that the intact eluted antibodies have reactivity for DR11, as well as other specificities with expected shared cross reactivity. Conclusion: These data demonstrate the coupling of native class II molecules to an affinity matrix, the select removal of antibodies from a patient sample, and the recovery of antibodies specific for a class II HLA molecule. This approach has notable potential applications, including direct measurement of antibody characteristics, use of HLA antibodies in cell culture systems, and potentially even for therapeutic intervention.
Ouabain-like activity in human cerebrospinal fluid. (1983)

Human cerebrospinal fluid has been found to mimic the effect of ouabain on net Na+ efflux and 86Rb+ influx across erythrocyte membranes and on the in vitro activity of a purified Na+/K+-ATPase (ATP phosphohydrolase, EC 3.6.1.3) derived from canine kidney. These results indicate the possible existence in human cerebrospinal fluid of an endogenous factor with ouabain-like activity, which might be linked to sodium metabolism.
Refracture rate after plate removal from the radial metaphysis. (2002)

The refracture rate at the metaphvsis of the radius after plate removal is lower than the rate after diaphyseal plate removal reported in the literature.
The Republic of Yemen Petroleum Overview (2001)

AbstractYemen located on the southwest corner of the Arabian Peninsula. It is known that Yemen is important to world energy markets because of its oil and natural gas resources as well as its strategic location at the Bab el - Mandab strait linking the Red Sea and the Gulf of Aden. Due to the Ministry of Oil and Mineral Resources (MOMR, 1998E) the Yemeni Oil Production was estimated to be 385 000 barrels per day (bbl/d), Oil Consumption was 69 000 bbl/d and Net Oil Exports was 316 000 barrels per day (bbl/d). On 1999 the Yemeni Proven Oil Reserves was estimated to be 4 billion barrels and the Natural Gas Reserves was estimated to be 16.9 trillion cubic feet (Tcf) (Ministry of Oil and Mineral Resources, 1999). At this time, many researchers, scientific expeditions and foreign companies are interested in Yemen. This paper discussed the petroleum overview of the Republic of Yemen. In my opinion, Yemen is not just going to attract all with its oil and gas discoveries in the near future, but also with its new geological data and information.
Look ahead batching to minimize earliness/tardiness measures in batch processes (2005)

Scheduling problems involving earliness/tardiness (E/T) measures have received significant attention in recent years. This type of problem became important with the advent of the just-in-time (JIT) manufacturing philosophy, where early or tardy deliveries are highly discouraged. In this paper we examine the single batch processing machine-scheduling problem in a dynamic environment for minimizing the E/T measures. We propose a look ahead batching (LAB) method where the scheduling decisions are made considering the arrival epochs and due dates of incoming lots, which are easily predictable in a computer integrated manufacturing environment, especially in the semiconductor industry. The results of the proposed method are compared with the dynamic batching heuristic (DBH) and next arrival control heuristic (NACH), which are look ahead strategies developed based on arrival information alone. The E/T performance is measured by minimization of the absolute sum of earliness and tardiness of the lots (|E|+|T|) and the minimization of their square sum (E/sup 2/+T/sup 2/). The steady state simulation results show that exploiting the knowledge of future arrivals and their due dates leads to a significant reduction in the E/T measures for tight and loose due date settings at two different utilization levels.
ChemInform Abstract: Observations on the Versatility of Methylenecyclopropanes as Olefinic Components in the Intramolecular Pauson—Khand Reaction. (1999)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Enhancement of the latent 3-isopropylmalate dehydrogenase activity of promiscuous homoisocitrate dehydrogenase by directed evolution (2010)

HICDH (homoisocitrate dehydrogenase), which is involved in lysine biosynthesis through α-aminoadipate, is a paralogue of IPMDH [3-IPM (3-isopropylmalate) dehydrogenase], which is involved in leucine biosynthesis. TtHICDH (Thermus thermophilus HICDH) can recognize isocitrate, as well as homoisocitrate, as the substrate, and also shows IPMDH activity, although at a considerably decreased rate. In the present study, the promiscuous TtHICDH was evolved into an enzyme showing distinct IPMDH activity by directed evolution using a DNA-shuffling technique. Through five repeats of DNA shuffling/screening, variants that allowed Escherichia coli C600 (leuB−) to grow on a minimal medium in 2 days were obtained. One of the variants LR5–1, with eight amino acid replacements, was found to possess a 65-fold increased kcat/Km value for 3-IPM, compared with TtHICDH. Introduction of a single back-replacement H15Y change caused a further increase in the kcat/Km value and a partial recovery of the decreased thermotolerance of LR5–1. Site-directed mutagenesis revealed that most of the amino acid replacements found in LR5–1 effectively increased IPMDH activity; replacements around the substrate-binding site contributed to the improved recognition for 3-IPM, and other replacements at sites away from the substrate-binding site enhanced the turnover number for the IPMDH reaction. The crystal structure of LR5–1 was determined at 2.4 Å resolution and revealed that helix α4 was displaced in a manner suitable for recognition of the hydrophobic γ-moiety of 3-IPM. On the basis of the crystal structure, possible reasons for enhancement of the turnover number are discussed.
Rear face hardware replacement 105-B, D, DR, F, and H (1963)

Prior to the use of venturis on the front of the B, D, DR, P, and H Reactors, much of the pressure and velocity heads developed by the 190 process water pumps were used in overcoming built-in frictional losses in the system external to the process tubes. As Reactor Technology was gained, changes were continually taking place. Venturis replaced orifices installed during original construction. Hydraulically efficient front nozzles, front pigtails, front adaptors, and a second generation of front venturis were designed and installed as a part of Project CG-558. Process changes, such as eliminating upstream dummy charges, were also made to reduce the energy dissipation due to frictional losses. The pressure drop across the active section of a fuel charge was reduced by the internal flow passage in the I&E elements. Little has been done to date, however, to reduce the pressure drops in the rear face fittings. This report is intended to review some of these still existent pressure restrictions and to discuss possible future courses of action.
American Luthier, Carleen Hutchins—The Art & Science of the Violin (2016)

First Page
Digital Clay Force Observer Design and Shape Editing Concept (2009)

Digital Clay is a novel fluid-driven device for shape input/output that potentially has wide application in the areas of engineering, sciences, medicine, military, entertainment etc. Shape display is achieved with the current 5×5 prototype but this research seeks to expand its haptic capability with force feedback, but the absence of pressure/force sensors requires finger force estimation. In this paper, a velocity estimator and a mathematical model are derived for the design of a force observer. A brief overview of the current 5×5 prototype at the Georgia Institute of Technology and a new concept for shape editing are also given.
A new synthesis of aldehydes via silane reduction of nitrilium ions (1974)

J. L. Fry, J. Chem. Soc., Chem. Commun., 1974, 45a DOI: 10.1039/C3974000045A
A Vacuum System for the Argonne 6 GeV Synchrotron Light Source (1985)

The ANL vacuum system for the 6 GeV light source storage ring features non-evaporable strip getter pumps for uniform pumping around the ring within a gas desorption antechamber, and it also features lumped getter pumping directly under and above crotch radiation absorbers that are positioned after each bending magnet. Based on experiments at ANL in 1980 and by others, the technical and economical advantages have been established for the use of the distributed NeG pumps of non-magnetic strips coated with a nonevaporable Zr Al getter matrix. The NeG strip pump lifetime approaches ten years. The antechamber improves the isolation of the gas desorption process from the main beam chamber and beam. The combination oE these vacuum techniques; the NeG strip getter pumps, the gas desorption antechambers, and the lumped ion and lumped getter pumping provide a unique and reliable system for maintaining long beam lifetime.
Effect of Clarithromycin on Nasal Mucus Properties in Healthy Subjects and in Patients With Purulent Rhinitis (1997)

Erythromycin inhibits mucus glycoconjugate secretion from airway cells in vitro and may increase mucus clearance in patients with asthma or diffuse panbronchiolitis. Because mucus hypersecretion is common in purulent rhinitis, we questioned whether clarithromycin would change the properties of nasal mucus from subjects without sinus disease and from patients with acute purulent rhinitis. Nasal secretions were collected before and after nasal methacholine challenge from 10 adults without nasal symptoms and without methacholine from 10 patients with purulent rhinitis. After 2 wk of oral clarithromycin (500 mg twice daily), secretions were again collected from both groups. Secretions were analyzed for viscoelasticity, cohesion, hydration, and ciliary and airflow (sneeze) transportability. Compared with secretions from healthy subjects, rhinitis secretions had decreased wettability (contact angle on Teflon 100 degrees versus 84.67 degrees; p = 0.001), increased cohesion (36.8 versus 24.3 mm; p = 0.003), decreased sneeze clearability (20.6 versus 32 mm; p = 0.04), and increased percent solids (4.61 versus 2.82%; p = 0.04). After clarithromycin, the rheology, hydration, cohesion, and transportability of the rhinitis secretions were similar to those of the postclarithromycin secretions from the healthy subjects. Secretion volume also decreased (500.1 versus 28.3 mg; p = 0.01), and mucociliary transportability increased by 30% (0.76 versus 0.99; p = 0.005). Although clarithromycin reduced mucus secretion in both rhinitis patients and normal subjects, it did not alter the secretory response to methacholine.
High solids sample introduction for flame atomic absorption analysis (1977)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTHigh solids sample introduction for flame atomic absorption analysisR. C. Fry and M. B. DentonCite this: Anal. Chem. 1977, 49, 9, 1413–1417Publication Date (Print):August 1, 1977Publication History Published online1 May 2002Published inissue 1 August 1977https://pubs.acs.org/doi/10.1021/ac50017a031https://doi.org/10.1021/ac50017a031research-articleACS PublicationsRequest reuse permissionsArticle Views96Altmetric-Citations28LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Antiviral activity of ribavirin on influenza infection in ferrets (1975)

The effect of l-β-D-ribofuranosyl-1-2, 4-triazoIe-3-carboxamide (Ribavirin) on influenza virus infection was studied in ferrets. Ferrets were given lOOmg/kg of ribavirin intraperitoneally 1 day before, 2 h before and daily for 5 days following intranasal infection with 103.0 ferret infective doses of A/Port Chalmers/73 virus. Ribavirin-treated animals showed no temperature response, no increase of nasal wash protein and did not produce nasal antibody following virus infection indicating an inhibitory effect on virus infection. In addition, drug-treated ferrets had markedly reduced titres of virus in nasal washings and did not produce serum antibody following virus infection. The absence of an antibody response in the presence of virus in the upper respiratory tract was attributed to an immunosuppressive action of the compound.
A phase 1 multidose study of SGN-30 immunotherapy in patients with refractory or recurrent CD30+ hematologic malignancies (2007)

Phase 1 testing of SGN-30, a chimeric monoclonal antibody for the treatment of CD30(+) malignancies, was conducted in a multicenter study. To explore the safety profile and establish the maximum tolerated dose (MTD), 24 patients with refractory or relapsed Hodgkin lymphoma or CD30(+) non-Hodgkin lymphoma received 6 weekly doses of intravenous SGN-30 at 4 dose levels (2, 4, 8, or 12 mg/kg). Serum concentrations of SGN-30 rose rapidly and were dose dependent. Adverse events were mild, with nausea, fatigue, and fever attributed to study treatment. One episode of hypersensitivity rash was reported. The MTD was not reached. Serious adverse events included herpes zoster (n = 2), influenza, and pneumonia. One patient with cutaneous anaplastic large cell lymphoma (8 mg/kg) achieved a complete response. Six patients, of whom 4 had Hodgkin lymphoma, achieved stable disease with durations ranging from 6 to 16 months. The pharmacokinetic profile of SGN-30 showed a biphasic disposition, and estimated half-lives ranging between 1 to 3 weeks. The 6 weekly infusions of SGN-30 resulted in approximately 2- to 3-fold accumulation in serum exposures consistently across the dose range. These results demonstrate that weekly administration of SGN-30 is safe and has modest clinical activity in patients with CD30(+) tumors. This trial is registered at http://www.ClinicalTrials.gov as no. NCT00051597.
Semantic Description of Multimodal Devices: Modelling and Evaluation (2008)

The heterogeneous, dynamic nature of current communication environments necessitates that all system components that form part of a personalisation framework should be context aware. To ensure context enabled interoperation, a shared, formalised specification of devices and services in the ambient environment is a must. With this aim, this paper presents an ontology model that captures the semantics of the multimodal devices and services in the mobile ad-hoc environment. The approach is validated using available metrics and compared to existing approaches, both through subjective feature-based evaluation and metrics’ calculations. This paper also extends the metrics’ usability by extending the analysis to interoperability with application logic and domain capture.
[The Achilles tendon in sports]. (1995)

Achillodynia is a generic term for various types of ailments in the region of the Achilles tendon. For adequate therapy a specific diagnosis is absolutely necessary. Besides an accurate anamnesis and the right choice of terrain and shoes, as well as a clinical examination where one has to specifically keep an eye on muscular imbalance between the gastrocnemius and the soleus muscle and disorders of the ligamentous control of the calcaneus caused by fibular ligament instabilities, a procedure such as radiology, ultrasound, and MR imaging is inevitable. From the differential diagnosis point of view a distinction between peritendinitis, mechanically triggered bursitis (calcaneal and subachilles), bony alterations of the calcaneus (calcaneus spur, Haglund exostosis persistent nucleus of the apophysis, fatigue fracture, etc) and a partial or total rupture (a one-time occurrence or multiple occurrences) has to be made. Occasionally, entrapment of the ramus calcaneus of the sural nerve causes calcaneal pain. If clinically not confirmed, lumbar pain ought to be taken into consideration (discopathy, Bechterew disease, etc). Metabolic disorders (especially uric acid) and underlying rheumatic diseases must be excluded. The therapy of achillodynia includes local and peroral antiphlogistic medication as a concomitant measure. More important is the causal influence of etiological factors, i.e., the correction of muscular imbalance, ensuring control of the calcaneus through bandages and adjustment of sport shoes, changes in training buildup and exercise intensity, just to mention a few. If necessary, surgically splitting the peritendineum, sanitation of a partial rupture, bursectomy and removal of mechanically obstructive exostosis must be done.
Fallacies And Facts In Design For Fire Resistance (2001)

This paper exposes a number of fallacies about fire resistance in buildings. Much of the conventional wisdom is incorrect. By examining these fallacies, the paper asserts that there is a legitimate role for fire resisting elements in any building fire safety strategy, despite increasing reliance being placed on sprinklers and other active fire protection systems. The paper explores many aspects of fire severity and fire resistance as applied to modern building design, pointing out that there is much more to fire safety than providing buildings with code-specified fire resistance, and that rational consideration of all factors is necessary.
The Design of a Molecular Assembly Line Based on Biological Molecules (2003)

Thesis (S.M.)--Massachusetts Institute of Technology, School of Architecture and Planning, Program in Media Arts and Sciences, 2003.