gebhart/patrepeval huggingface dataset:
Vinyl Sulfone Bifunctional Tag Reagents for Single-Point Modification of Proteins (2010)

The introduction of multiple labels onto biomolecules is a challenge. We report herein the synthesis of vinyl sulfone derivatized bifunctional tag single-attachment-point reagents (BTSAP) bearing biotin and a fluorescent tag and their applications in proteins for the introduction of multiple labels by means of the Michael-type addition of the electrophilic vinyl sulfone group. These BTSAP reagents were easily synthesized by a two-step chemical strategy involving the preparation of alkyne vinyl sulfone derivatized tags (AVST) and subsequent click CuAAC attachment of a second azide functionalized tag. The direct coupling of BTSAP reagents with the low reactive protein horseradish peroxidase (HRP) turned it into a dual reporter group (i.e., fluorescence and peroxidase activity) that may be coupled to any recognition system via biotin-avidin affinity. The AVST compounds are not mere synthetic intermediates for the preparation of BTSAP reagents but valuable clickable self-reporting compounds that allow the simultaneous introduction in proteins of an alkyne function and labeling when conjugated via the vinyl sulfone group. The implementation of these clickable AVST compounds in a CuAAC-based sequential approach also allows attainment of the dual labeling of HRP. This approach yields equivalent results in terms of fluorescent labeling, specific activity, and functionality of the biotin tag when compared with the direct bifunctional labeling by the BTSAP reagent. However, for life science this direct approach is more convenient since it avoids the use of copper catalysis, overcoming the toxicity drawback of this metal in biological systems.
Pro-inflammatory effects of the aminobisphosphonate ibandronate in vitro and in vivo (1999)

The enhanced production of TNF-alpha by ibandronate-treated PBMC in vitro involves stimulation of adherent monocytes by IFN-gamma prior to LPS-induced activation. Similar cellular interactions may be involved in the pro-inflammatory effects of ibandronate in vivo.
Probing the Sensory Property of Perylenediimide Derivatives in Hydrazine Gas: Core-Substituted Aromatic Group Effect (2014)

In this contribution, four perylenediimide derivatives (PTCDIs) with different core-substituted aromatic groups were prepared. Studies on their sensing properties in hydrazine vapor (10 ppm) suggested ∼5 orders of the magnitude in increased current for core-phenyl-substituted DEY was achieved and this value is 9, 9, and 24 times higher than that of core-pyridyl-substituted DSPY, DFPY, and DTPY, respectively. The differential response to the hydrazine vapor is less dependent on their surface area and morphologies. The lower LUMO energy and activation energy with smaller interplanar spacing allows DEY highly efficient sensing performance. A similar face-face packing mode and LUMO energy of DSPY and DFPY lead to both of them exhibiting the same sensing performance, while higher LUMO energy and head-to-tail packing modes with a greater interplanar spacing induce the less-efficient sensing performance of DTPY sensors. Discussions for structure-function relationships suggested that aromatic groups in the bay region have significant impact on PTCDI sensing performance by modulating energy level, interplanar spacing, and stacking modes.
Increased instrument intelligence--can it reduce laboratory error? (2005)

Recent literature has focused on the reduction of laboratory errors and the potential impact on patient management. This study assessed the intelligent, automated preanalytical process-control abilities in newer generation analyzers as compared with older analyzers and the impact on error reduction. Three generations of immuno-chemistry analyzers were challenged with pooled human serum samples for a 3-week period. One of the three analyzers had an intelligent process of fluidics checks, including bubble detection. Bubbles can cause erroneous results due to incomplete sample aspiration. This variable was chosen because it is the most easily controlled sample defect that can be introduced. Traditionally, lab technicians have had to visually inspect each sample for the presence of bubbles. This is time consuming and introduces the possibility of human error. Instruments with bubble detection may be able to eliminate the human factor and reduce errors associated with the presence of bubbles. Specific samples were vortexed daily to introduce a visible quantity of bubbles, then immediately placed in the daily run. Errors were defined as a reported result greater than three standard deviations below the mean and associated with incomplete sample aspiration of the analyte of the individual analyzer Three standard deviations represented the target limits of proficiency testing. The results of the assays were examined for accuracy and precision. Efficiency, measured as process throughput, was also measured to associate a cost factor and potential impact of the error detection on the overall process. The analyzer performance stratified according to their level of internal process control The older analyzers without bubble detection reported 23 erred results. The newest analyzer with bubble detection reported one specimen incorrectly. The precision and accuracy of the nonvortexed specimens were excellent and acceptable for all three analyzers. No errors were found in the nonvortexed specimens. There were no significant differences in overall process time for any of the analyzers when tests were arranged in an optimal configuration. The analyzer with advanced fluidic intelligence demostrated the greatest ability to appropriately deal with an incomplete aspiration by not processing and reporting a result for the sample. This study suggests that preanalytical process-control capabilities could reduce errors. By association, it implies that similar intelligent process controls could favorably impact the error rate and, in the case of this instrument, do it without negatively impacting process throughput. Other improvements may be realized as a result of having an intelligent error-detection process including further reduction in misreported results, fewer repeats, less operator intervention, and less reagent waste.
Nuclear Magnetic Resonance (1956)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNuclear Magnetic ResonanceJ. G. AstonCite this: J. Am. Chem. Soc. 1956, 78, 20, 5455Publication Date (Print):October 1, 1956Publication History Published online1 May 2002Published inissue 1 October 1956https://pubs.acs.org/doi/10.1021/ja01601a091https://doi.org/10.1021/ja01601a091research-articleACS PublicationsRequest reuse permissionsArticle Views110Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
EMG classification for prehensile postures using cascaded architecture of neural networks with self-organizing maps (2004)

Electromyograph (EMG) features have the properties of large variations and nonstationary issue in the classification of EMG is the classifier design. The major goal of this paper is to develop a classifier for the classification of eight kinds of prehensile postures to achieve high classification rate and reduce the online learning time. The cascaded architecture of neural networks with feature map (CANFM) is proposed to achieve the goal. The CANFM is composed of two kinds of neural networks: an unsupervised Kohonen's self-organizing map (SOM), and a supervised multi-layer feedforward neural network. Experimental results show that by extracting EMG features, forth-order autoregressive model (ARM) and histogram of EMG signals (IEMG), as inputs, the proposed CANFM can obtain and remain high classification rates compared with other classifiers, including k-nearest neighbor method (K-NN), fuzzy K-NN algorithm, and back-propagation neural network (BPNN) in several online testing.
Full-duplex fast initializing digital subscriber loop echo cancellers (1994)

In this paper, we propose a novel approach to fast initializing digital subscriber loop (DLS) echo cancellers using full-duplex transmitted training sequences. The important difference between our method and the previous half-duplex fast training schemes is that we can simultaneously estimate the impulse responses of the echo paths of both ends rather than estimate them separately. Therefore, the initialization time is greatly reduced. The effects of the noise and symbol rate offset between two ends are examined in terms of both mean-square error (MSE) and signal to noise ratio (SNR), and the results show that the new algorithm is robust in the presence of noise and symbol rate offset.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Catalytic Enantioselective Hetero Diels−Alder Reactions of α,β-Unsaturated Acyl Phosphonates with Enol Ethers <i>J. Am. Chem. Soc.</i> <b>1998</b>, <i>120</i>, 4895−4896 (1998)

ADVERTISEMENT RETURN TO ISSUEPREVAddition/CorrectionNEXTCatalytic Enantioselective Hetero Diels−Alder Reactions of α,β-Unsaturated Acyl Phosphonates with Enol Ethers J. Am. Chem. Soc.1998, 120, 4895−4896David A. Evans and Jeffrey S. JohnsonCite this: J. Am. Chem. Soc. 1998, 120, 24, 6197Publication Date (Web):June 5, 1998Publication History Published online5 June 1998Published inissue 1 June 1998https://pubs.acs.org/doi/10.1021/ja9855085https://doi.org/10.1021/ja9855085correctionACS PublicationsCopyright © 1998 American Chemical Society. This publication is available under these Terms of Use. Request reuse permissions This publication is free to access through this site. Learn MoreArticle Views762Altmetric-Citations1LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail PDF (19 KB) Get e-Alertsclose Get e-Alerts
Orp1, a member of the Cdc18/Cdc6 family of S-phase regulators, is homologous to a component of the origin recognition complex. (1995)

cdc18+ of Schizosaccharomyces pombe is a periodically expressed gene that is required for entry into S phase and for the coordination of S phase with mitosis. cdc18+ is related to the Saccharomyces cerevisiae gene CDC6, which has also been implicated in the control of DNA replication. We have identified a new Sch. pombe gene, orp1+, that encodes an 80-kDa protein with amino acid sequence motifs conserved in the Cdc18 and Cdc6 proteins. Genetic analysis indicates that orp1+ is essential for viability. Germinating spores lacking the orp1+ gene are capable of undergoing one or more rounds of DNA replication but fail to progress further, arresting as long cells with a variety of deranged nuclear structures. Unlike cdc18+, orp1+ is expressed constitutively during the cell cycle. cdc18+, CDC6, and orp1+ belong to a family of related genes that also includes the gene ORC1, which encodes a subunit of the origin recognition complex (ORC) of S. cerevisiae. The products of this gene family share a 250-amino acid domain that is highly conserved in evolution and contains several characteristic motifs, including a consensus purine nucleotide-binding motif. Among the members of this gene family, orp1+ is most closely related to S. cerevisiae ORC1. Thus, the protein encoded by orp1+ may represent a component of an Sch. pombe ORC. The orp1+ gene is also closely related to an uncharacterized putative human homologue. It is likely that the members of the cdc18/CDC6 family play key roles in the regulation of DNA replication during the cell cycle of diverse species from archaebacteria to man.
Bursting and calcium oscillations in pancreatic β-cells: specific pacemakers for specific mechanisms (2010)

Oscillatory phenomenon in electrical activity and cytoplasmic calcium concentration in response to glucose are intimately connected to multiple key aspects of pancreatic β-cell physiology. However, there is no single model for oscillatory mechanisms in these cells. We set out to identify possible pacemaker candidates for burst activity and cytoplasmic Ca(2+) oscillations in these cells by analyzing published hypotheses, their corresponding mathematical models, and relevant experimental data. We found that although no single pacemaker can account for the variety of oscillatory phenomena in β-cells, at least several separate mechanisms can underlie specific kinds of oscillations. According to our analysis, slowly activating Ca(2+)-sensitive K(+) channels can be responsible for very fast Ca(2+) oscillations; changes in the ATP/ADP ratio and in the endoplasmic reticulum calcium concentration can be pacemakers for both fast bursts and cytoplasmic calcium oscillations, and cyclical cytoplasmic Na(+) changes may underlie patterning of slow calcium oscillations. However, these mechanisms still lack direct confirmation, and their potential interactions raises new issues. Further studies supported by improved mathematical models are necessary to understand oscillatory phenomena in β-cell physiology.
A Rule based Approach to Word Lemmatization (2004)

Lemmatization is the process of finding the normalized form of a word. It is the same as looking for a transformation to apply on a word to get its normalized form. The approach presented in this paper focuses on word endings: what word suffix should be removed and/or added to get the normalized form. This paper compares the results of two word lemmatization algorithms, one based on if-then rules and the other based on ripple down rules induction algorithms. It presents the problem of lemmatization of words from Slovene free text and explains why the Ripple Down Rules (RDR) approach is very well suited for the task. When learning from a corpus of lemmatized Slovene words the RDR approach results in easy to understand rules of improved classification accuracy compared to the results of rule learning achieved in previous work.
Combining Preprocessor Slicing with C/C++ Language Slicing (2008)

Slicing C programs has been one of the most popular ways for the implementation of slicing algorithms; out of the very few practical implementations that exist many deal with this programming language. Yet, preprocessor related issues have been addressed very marginally by these slicers, despite the fact that ignoring (or handling poorly) these constructs may lead to serious inaccuracies in the slicing results and hence in the comprehension process. Recently, an accurate slicing method for preprocessor related constructs has been proposed which - when combined with existing C/C++ language slicers - can provide a more complete comprehension of these languages. In this paper, we overview our approach for this combination and report its benefits in terms of the completeness of the resulting slices.
The Combination of Nebivolol plus Pravastatin is Associated with a More Beneficial Metabolic Profile Compared to that of Atenolol plus Pravastatin in Hypertensive Patients with Dyslipidemia: A Pilot Study (2003)

Nebivolol, a selective beta1-lipophilic blocker, achieves blood pressure control by modulating nitric oxide release in addition to b-blockade. This dual mechanism of action could result in minimum interference with lipid metabolism compared to atenolol, a classic beta1-selective blocker. Hypertensive patients commonly exhibit lipid abnormalities and frequently require statins in combination with the anti-hypertensive therapy. We conducted this trial in order to clarify the effect on the metabolic profile of beta-blocker therapy with atenolol or nebivolol alone, or in conjunction with pravastatin. Thirty hypertensive hyperlipidemic men and women (total cholesterol >240 mg/dL [6.2 mmol/L], low-density lipoprotein cholesterol >190 mg/dL [4.9 mmol/L], triglycerides <500 mg/dL [5.6 mmol/L]) were separated in two groups. One group consisted of 15 subjects on atenolol therapy (50 mg daily), and the other group included 15 subjects on nebivolol therapy (5 mg daily). After 12 weeks of beta-blocker therapy, pravastatin (40 mg daily) was added in both groups for another 12 weeks. Atenolol significantly increased triglyceride levels by 19% (P=.05), while nebivolol showed a trend to increase high-density lipoprotein cholesterol by 8% (NS) and to decrease triglyceride levels by 5% (NS). Atenolol significantly increased lipoprotein(a) by 30% (P=.028). Fibrinogen levels were equally and not significantly decreased in both groups by 9% and 7%, respectively. Furthermore, atenolol and nebivolol decreased serum high-sensitivity C-reactive protein levels by 14% (P=.05) and 15% (P=.05), respectively. On the other hand, both atenolol and nebivolol showed a trend to increase homocysteine levels (NS) by 13% and 11%, respectively. Although uric acid levels remained the same, atenolol significantly increased the fractional excretion of uric acid by 33% (P=.03). Following nebivolol administration, glucose levels remained the same, while insulin levels were reduced by 10% and the HOMA index (fasting glucose levels multiplied by fasting insulin levels and divided by 22.5) was reduced by 20% (P=.05). There were no significant differences between the two patient groups in the measured parameters after the administration of beta-blockers, except for triglycerides (P<.05) and the HOMA index (P=.05). The addition of pravastatin to all patients (n=30) decreased total cholesterol by 21% (P<.001), low-density lipoprotein cholesterol by 28% (P<.001), apolipoprotein-B by 22% (P<.001), apolipoprotein-E by 15% (P=.014) and lipoprotein(a) levels by 12% (P=.023). Moreover, homocysteine levels and C-reactive protein were reduced by 17% (P=.05) and 43% (P=.05), respectively. We conclude that nebivolol seems to be a more appropriate therapy in hypertensive patients with hyperlipidemia and carbohydrate intolerance. Finally, the addition of pravastatin could further correct the well-established predictors of cardiovascular events.
Resin Sand Consolidation In Baram Delta, Sarawak. Case Study On Resin Performance versus Internal Gravelpacking With Acid Prepacking (1999)

Abstract Chemical sand consolidation (SCON) is a type of sand exclusion which withholds unconsolidated formation from disintegrating and producing loose grains into the wellbore. The produced sand can lead to plugging off completion accessories and eroding surface facilities which eventually, over long period of time, will hamper production of a well. Most of the time, a well needs to be shut in and some oil reserves will be left behind the casing. It requires investment in bailing out the sand from the well or may be to the extend of incurring high investment to workover the well. Sand exclusion with internal gravelpacking (IGP) has been proven in Baram Delta field, Sarawak since early 60's. In early 90's, acidprepacking was introduced as part of gravelpacking job for better placement of gravels in the perforation tunnels. Historical performances show that the well has less skin than the conventional gravelpacking as the acid used can clean up the perforation tunnels and place the gravels inside them. The technique proved that the well flushed with acid prior to gravelpack job can sustain production much longer and at higher rate. There are many chemical SCON (e.g. phenolic, epoxy, and furan) available in the market, but the project team decided to use furan resin mainly due to its economic viability. At the end of 1998, furan resin was applied in one of the Baram wells and it had improved production rate and much less skin compared to the well using IGP in the same reservoir package. Sand treated with resin is capable of delivering about 1000 bopd from 15 ft. of perforation interval compared to only 400 bopd in 49 ft. IGP perforated interval in another typical well. In Baram Delta case even with snort perforation interval, the sand treated with resin can potentially deliver higher production than the sand controlled with IGP. The success of the pilot furan resin application are mainly due to the combination of a good drainage point selection, drilling practices, and cooperation between the production team and the project team during the production phase.
A Method for Attenuation Correction in Radionuclide Computed Tomography (1978)

The development of algorithms for Radionuclide Computed Tomography (RCT) is complicated by the presence of attenuation of gamma-rays inside the body. Some of the existing RCT reconstruction algorithms apply approximation formulas to the projection data for attenuation correction, while others take attenuation into account through some iterative procedures. The drawbacks of these algorithms are that the approximation formulas commonly used are generally inadequate and the iterative procedures are usually very time-consuming. The method for attenuation correction in RCT, which we propose, applied a simple, effective two-step procedure to the uncorrected image. In this procedure the filtered back-projection algorithm is used for its fast speed. A simple mathematical basis and description of the procedure together with some illustrative computer results are given in this paper.
Determination of foil thickness (1966)

Abstract It is shown how the apparent lengths of dislocations as seen in a transmission electron micrograph of a thin foil can be used to determine the foil thickness. The necessary conditions are discussed and a practical example given.
Domain-specific language integration with compile-time parser generator library (2010)

Smooth integration of domain-specific languages into a general purpose host language requires absorbing of domain code written in arbitrary syntax. The integration should cause minimal syntactical and semantic overhead and introduce minimal dependency on external tools. In this paper we discuss a DSL integration technique for the C++ programming language. The solution is based on compile-time parsing of the DSL code. The parser generator is a C++ template metaprogram reimplementation of a runtime Haskell parser generator library. The full parsing phase is executed when the host program is compiled. The library uses only standard C++ language features, thus our solution is highly portable. As a demonstration of the power of this approach, we present a highly efficient and type-safe version of printf and the way it can be constructed using our library. Despite the well known syntactical difficulties of C++ template metaprograms, building embedded languages using our library leads to self-documenting C++ source code.