gebhart/patrepeval huggingface dataset:
A study on the membrane depolarization of skeletal muscles caused by a scorpion toxin, sea anemone toxin II and crotamine and the interaction between toxins (1983)

Quinquestriatus toxin (QTX) isolated from the venom of a scorpion (Leiurus quinquestriatus) and sea anemone (Anemonia sulcata) toxin II enhanced the twitch response of the rat and mouse diaphragms and like crotamine (isolated from the venom of Crotalus durissus terrificus) caused spontaneous fasciculation of the muscle. Trains of action potentials in muscles at 70-250 Hz, which could not be antagonized by (+)-tubocurarine, were triggered by single stimulation or occurred spontaneously after treatment with these toxins. QTX and toxin II prolonged the rat muscle action potential 3 to 4 fold whereas crotamine prolonged the action potential by only 30%. The membrane potential was depolarized from about -82 mV to -55 mV by crotamine 2 micrograms ml-1, -41 mV by toxin II 5 micrograms ml-1 and to -50 mV by QTX 1 microgram ml-1. The concentrations to induce 50% maximal depolarization (K0.5) were 0.07, 0.15 and greater than 0.4 microgram ml-1, respectively, for QTX, crotamine and toxin II, whereas the rates of depolarization were in the order toxin II greater than or equal to crotamine greater than QTX. The depolarizing effects of crotamine and QTX, but not of toxin II, were saturable. The depolarizing effects of all three toxins were irreversible whereas the membrane potential could be restored by tetrodotoxin non-competitively. Simultaneous treatment with crotamine and QTX or crotamine and toxin II at concentrations below K0.5 caused only additive effects on depolarization. When the muscle was depolarized by pretreating with a saturating concentration of crotamine, the onset of depolarization by QTX was greatly retarded whereas that by toxin II was unaffected. Action potentials were further prolonged in both cases. 8 It is inferred that all three peptide toxins act at sites on the sodium channel and the binding sites for QTX and crotamine overlap to a considerable extent. On the other hand, the site for toxin II appears not to overlap with that of crotamine but may overlap with that of QTX.
Analysis of a Single-Ended Push-Pull Audio Amplifier (1953)

This paper deals with a theoretical circuit analysis of a single-ended push-pull audio amplifier. Linear tube characteristics and small signals are assumed. The problem of impedance matching is first discussed. The properties and the requirements of a satisfactory driver stage are analyzed, and an output stage using an impedance matching output transformer is discussed. The effects of the plate-to-ground capacitance of the driver stage on the frequency-gain characteristic and of the choice of the tube and circuit parameters are analyzed. The paper concludes with some experimental results indicative of the inherant properties of the amplifier.
Race and Gender Dynamics as an Organisational Defence against Anxiety within a Specialist Multi-Disciplinary Child and Family Assessment Service (2012)

The article discusses the complex dynamics of difference operating within the context of a new service that had been set up by a group of black child protection experts with a specialism in working with black children and families who had been brought before the courts due to incidents of child abuse and neglect. Although expanding at an impressive rate the organisation was seriously struggling to understand the graveness of the situation of it not having established an appropriate business infrastructure, which was essential in order for it to survive. The organisation was caught up in a complex and highly defended social defence system/psychic retreat that struggled to take in appropriate inputs which was jeopardising its future. The inputs of outsiders had become symbolically equated with conquest and colonisation. The organisation struggled to recognise necessary differences and was overly attached to notions of sameness that extended not only to race and gender but also in terms of roles within the organisation and the inputs of consultants. The threat of resignation by the only male director brought the organisation into crisis that enabled it to allow input, thereby installing a symbolic law of the father that enabled sufficient ability to symbolise and movement out of a basic assumption paranoid-schizoid culture that was less dominated by symbolic equations into a more mature, depressive work group that was able to rise to Oedipal demands and, therefore, more able to appreciate difference, accountability and dependency.
Altered morphology produced by ftsZ expression in Corynebacterium glutamicum ATCC 13869 (2005)

Corynebacterium glutamicum is a Gram-positive bacterium that lacks the cell division FtsA protein and actin-like MreB proteins responsible for determining cylindrical cell shape. When the cell division ftsZ gene from C. glutamicum (ftsZ(Cg)) was cloned in different multicopy plasmids, the resulting constructions could not be introduced into C. glutamicum; it was assumed that elevated levels of FtsZ(Cg) result in lethality. The presence of a truncated ftsZ(Cg) and a complete ftsZ(Cg) under the control of Plac led to a fourfold reduction in the intracellular levels of FtsZ, generating aberrant cells displaying buds, branches and knots, but no filaments. A 20-fold reduction of the FtsZ level by transformation with a plasmid carrying the Escherichia coli lacI gene dramatically reduced the growth rate of C. glutamicum, and the cells were larger and club-shaped. Immunofluorescence microscopy of FtsZ(Cg) or visualization of FtsZ(Cg)-GFP in C. glutamicum revealed that most cells showed one fluorescent band, most likely a ring, at the mid-cell, and some cells showed two fluorescent bands (septa of future daughter cells). When FtsZ(Cg)-GFP was expressed from Plac, FtsZ rings at mid-cell, or spirals, were also clearly visible in the aberrant cells; however, this morphology was not entirely due to GFP but also to the reduced levels of FtsZ expressed from Plac. Localization of FtsZ at the septum is not negatively regulated by the nucleoid, and therefore the well-known occlusion mechanism seems not to operate in C. glutamicum.
The use of bootstrapping by women entrepreneurs in positioning for growth (2006)

Abstract The number of women entrepreneurs is rising rapidly and many are creating substantial businesses. For most women-led ventures, growth is funded by personal investment and debt, although a small percentage draw on private equity investment to fuel high growth. Of those that seek growth, not only do they face higher obstacles in obtaining capital, but little is known about ways they position ventures for growth. This paper addresses the question: ‘How do women develop financing strategies to prove the business concept, meet early stage milestones, and demonstrate to external investors the value and potential of their businesses?’ Data are drawn from phone interviews with 88 US female entrepreneurs seeking an equity investment to grow their businesses. The analysis examines the correspondence between bootstrapping and stage of business development. Results show significant differences in the use of bootstrap options utilized by women-led ventures depending on stage of business development. Companies that have not achieved sales were more likely to emphasize bootstrapping to reduce labour, while those companies with greater sales were more likely to minimize cost of operations. Implications for future research and education are suggested.
Where to From Here for the Catholic Church- Recommendations 94 and 95 of the Redress and Civil Litigation Report (2018)

The Royal Commission into Institutional Responses to Child Sexual Abuse handed down its Final Report in December 2017. In 2015 it presented its interim Redress and Civil Litigation Report which contained final recommendations in relation to reform in civil litigation. Recommendations 94 and 95 of the Redress and Civil Litigation Report both directly and indirectly address the lack of legal entity for the Catholic Church in Australia and the problems this causes litigants seeking legal recompense. This paper considers the current legal status of the Catholic Church in Australia in light of the Recommendations.
Altered Electrical Properties in<i>Drosophila</i>Neurons Developing without Synaptic Transmission (2001)

We examine the role of synaptic activity in the development of identified Drosophila embryonic motorneurons. Synaptic activity was blocked by both pan-neuronal expression of tetanus toxin light chain (TeTxLC) and by reduction of acetylcholine (ACh) using a temperature-sensitive allele of choline acetyltransferase (Cha(ts2)). In the absence of synaptic activity, aCC and RP2 motorneurons develop with an apparently normal morphology and retain their capacity to form synapses. However, blockade of synaptic transmission results in significant changes in the electrical phenotype of these neurons. Specifically, increases are seen in both voltage-gated inward Na(+) and voltage-gated outward K(+) currents. Voltage-gated Ca(2+) currents do not change. The changes in conductances appear to promote neuron excitability. In the absence of synaptic activity, the number of action potentials fired by a depolarizing ramp (-60 to +60 mV) is increased and, in addition, the amplitude of the initial action potential fired is also significantly larger. Silencing synaptic input to just aCC, without affecting inputs to other neurons, demonstrates that the capability to respond to changing levels of synaptic excitation is intrinsic to these neurons. The alteration to electrical properties are not permanent, being reversed by restoration of normal synaptic function. Whereas our data suggest that synaptic activity makes little or no contribution to the initial formation of embryonic neural circuits, the electrical development of neurons that constitute these circuits seems to depend on a process that requires synaptic activity.
Optimization of self-acting herringbone-grooved journal bearings for maximum stability (1974)

Groove parameters were determined to maximize the stability of herringbone-grooved journal bearings. Parameters optimized were groove depth, width, length, and angle. Optimization was performed by using a small-eccentricity, infinite-groove analysis in conjunction with a previously developed Newton-Raphson procedure for bearings with the smooth member rotating or with the grooved member rotating at low compressibility numbers, and a newly developed vector technique for bearings with the grooved member rotating at high compressibility numbers. The design curves in this report enable one to choose the optimum bearing for a wide range of operating conditions. Compared with bearings optimized to maximize load capacity, bearings optimized for stability allow a thousandfold increase in bearing-supported mass in some cases before onset of instability, and lose no more than 77 percent of their load capacity in any case studied. Stability is much greater when the grooved member rotates.
Prognostic value of serum IL‐10 and soluble IL‐2 receptor levels in aggressive non‐Hodgkin's lymphoma (1994)

We investigated the prognostic significance of interleukin-10 (IL-10) and soluble interleukin-2 receptor (sIL-2r) levels in the pretreatment serum of 105 individuals with newly-diagnosed aggressive non-Hodgkin's lymphoma (NHL). Commercially available enzyme-linked immunoassay kits were used for cytokine and receptor measurements. Detectable levels of IL-10 were found in 42 (40%) patients at diagnosis, with no correlation with clinico-haematological parameters, but in no control samples (P < 0.001). Pretreatment concentrations of sIL-2r were markedly increased in individuals with NHL when compared to controls (2614 +/- 893 U/ml v 219 +/- 65 U/ml, P < 0.001), patients with stage III/IV presenting higher values than those with stage II disease (3885 +/- 1196 U/ml v 1732 +/- 646 U/ml, P < 0.001). No single parameter was associated with the achievement of complete remission, but the combination of elevated IL-10 and of sIL-2r greater than 3000 U/ml selected a subset of patients with a high probability of failing induction therapy (P < 0.001). Life-table analysis also indicated that patients with these characteristics have a significantly shorter event-free survival. In a multivariate analysis the combination of IL-10 with sIL-2r was found to have greater predictive strength than the combination of IL-10 with beta 2-microglobulin. We conclude that IL-10 and sIL-2r measurements can be expected to improve existing methods of risk assignment in aggressive NHL.
Quantitative proteomics study of the neuroprotective effects of B12 on hydrogen peroxide-induced apoptosis in SH-SY5Y cells (2016)

B12 belongs to the coumarin class of compounds that have been shown to have various physiological and pharmacological activities including anti-inflammatory, antibacterial, and antioxidant. In the present study, we characterised the neuroprotective effects of B12 against H2O2-induced neuronal cell damage in SH-SY5Y cells. Protein expression profiling in combination with pathway analysis was deployed to investigate the molecular events associated with the neuroprotective effects in human neuronal cells using a label-free quantitative proteomics approach. A total of 22 proteins were significantly differentially expressed in H2O2-damaged cells with or without B12 treatment. Bioinformatics analysis using the Cytoscape platform indicated that poly pyrimidine tract binding protein 1 (PTBP1) was highly associated with the protective effect, and western blotting verified that PTBP1 was up-regulated in H2O2 + B12 treatment group, compared with the H2O2 treated group. PTBP RNAi experiments knocked down PTBP expression, which cancelled out the protective effect of B12 on cell viability. Thus, we infer that B12 neuroprotective activity involves up-regulation of PTBP1 and its associated signalling networks following H2O2-induced apoptosis in SH-SY5Y cells. B12 or related compounds may prove to be useful therapeutic agents for the treatment of neurodegenerative diseases such as Alzheimer's and Parkinson's.
Bragg reflection waveguide composite structures (1990)

The Bragg reflection waveguide directional coupler is analyzed rigorously in terms of the normal modes of the complete structure. A perturbation analysis leading to an explicit expression for the coupling constant in a general configuration with nearly degenerate modes is performed. The results are compared to those for a conventional directional coupler structure. It is shown that the bandlike dispersion diagram arising from the periodic lateral confining media is responsible for the unique features of the coupling constant and coupling length that are observed.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Monoclonal antibody‐mediated inhibition of adhesion of <i>Candida albicans</i> and <i>Candida dubliniensis</i> to human epithelial cells (2009)

The aim of this study was to assess the role of whole saliva, four saliva-derived preparations, and six monoclonal antibodies (mAbs), directed against components of the cell wall of Candida albicans, on the adhesion of C. albicans and Candida dubliniensis to human epithelial cells (HEC). C. albicans serotype A NCPF 3153 and C. albicans serotype B ATCC 90028 showed higher adhesion to HEC than C. dubliniensis NCPF 3949. Pooled whole saliva was more efficient than salivary secretory immunoglobulin A, partially purified by chromatography, at inhibiting the adhesion of C. albicans serotype A NCPF 3153 to HEC. Monoclonal antibodies C7, 14-8, and 26G7 were the most potent inhibitors of adhesion. Our results show that mAbs can mimic the inhibition of adhesion of C. albicans to HEC that is mediated by human saliva.
A new all-zero 4×4 block determination rule for integer transform and quantization in AVS-M encoder (2006)

A new all-zero block determination rule was used to reduce the complexity of the AVS-M encoder. It reuses the sum of absolute difference of 4×4 block obtained from motion estimation or intra prediction as parameters so that the determination threshold need to be computed only once when quantization parameter (QP) is invariable for given video sequence. This method avoids a lot of computation for transform, quantization, inverse transform, inverse quantization and block reconstruction. Simulation results showed that it can save about 20%～50% computation without any video quality degradation.
Heart disease in Turner's syndrome. (1988)

Fifty-five consecutive patients with Turner's syndrome and different karyotypes underwent full cardiological evaluation (physical examination, electrocardiogram, chest X-ray and echocardiogram). Congenital heart disease was found in 17%. When compared with the general population, a significantly higher incidence was present only for aortic coarctation and partial anomalous pulmonary venous drainage (p less than 0.001). Among cardiac anomalies in Turner's syndrome patients, aortic malformations (aortic coarctation, aortic stenosis, bicuspid aorta) are the most frequent, followed by partial anomalous pulmonary venous drainage, the incidence of which has been much higher than that previously reported. We have observed that the most severe malformations are preferably found in 45,X, while the ring pattern is characterized by a very high incidence of bicuspid aorta. No anomaly was found in the patients with X isochromosome.
Detection and Quantification of Antibodies to Newcastle Disease Virus in Ostrich and Rhea Sera Using a Liquid Phase Blocking Enzyme-Linked Immunosorbent Assay (2000)

A liquid phase blocking ELISA (LPB-ELISA) was adapted for the detection and quantification of antibodies to Newcastle disease virus. Sera from vaccinated and unvaccinated commercial flocks of ostriches (Struthio camelus) and rheas (Rhea americana) were tested. The purified and nonpurified virus used as the antigen and the capture and detector antibodies were prepared and standardized for this purpose. The hemagglutination-inhibition (HI) test was regarded as the reference method. The cutoff point for the LPB-ELISA was determined by a two-graph receiver operating characteristic analysis. The LPB-ELISA titers regressed significantly (P < 0.0001) on the HI titers with a high correlation coefficient (r = 0.875). The two tests showed good agreement (kappa = 0.82; P < 0.0001), relative sensitivity (90.91%) and specificity (91.18%), and accuracy (91.02%), suggesting that they are interchangeable.
Impact of tumour associated macrophages in pancreatic cancer (2013)

During cancer progression, bone marrow derived myeloid cells, including immature myeloid cells and macrophages, progressively accumulate at the primary tumour site where they contribute to the establishment of a tumour promoting microenvironment. A marked infiltration of macrophages into the stromal compartment and the generation of a desmoplastic stromal reaction is a particular characteristic of pancreatic ductal adenocarcinoma (PDA) and is thought to play a key role in disease progression and its response to therapy. Tumour associated macrophages (TAMs) foster PDA tumour progression by promoting angiogenesis, metastasis, and by suppressing an anti-tumourigenic immune response. Recent work also suggests that TAMs contribute to resistance to chemotherapy and to the emergence of cancer stem-like cells. Here we will review the current understanding of the biology and the pro-tumourigenic functions of TAMs in cancer and specifically in PDA, and highlight potential therapeutic strategies to target TAMs and to improve current therapies for pancreatic cancer.
Biosensors for the detection of respiratory viruses: A review (2020)

The recent events of outbreaks related to different respiratory viruses in the past few years, exponentiated by the pandemic caused by the coronavirus disease 2019 (COVID-19), reported worldwide caused by SARS-CoV-2, raised a concern and increased the search for more information on viruses-based diseases. The detection of the virus with high specificity and sensitivity plays an important role for an accurate diagnosis. Despite the many efforts to identify the SARS-CoV-2, the diagnosis still relays on expensive and time-consuming analysis. A fast and reliable alternative is the use of low-cost biosensor for <i>in loco</i> detection. This review gathers important contributions in the biosensor area regarding the most current respiratory viruses, presents the advances in the assembly of the devices and figures of merit. All information is useful for further biosensor development for the detection of respiratory viruses, such as for the new coronavirus.
New entrants and the role of information technology: Case study: The Tele-Flower Auction in The Netherlands (1997)

This article discusses the successful entrance of Tele Flower Auction (TFA) into the Dutch flower industry, enabled by information technology (IT). Indeed, the development and introduction of TFA is one of the initiatives in response to import restrictions by the traditional Dutch flower auctions. TFA is an electronic alternative that enables buyers to trade at a distance; this alternative is currently exploited by an import organization called East African Flowers (EAF). This article aims to provide a better understanding of the success of TFA. It provides a descriptive framework for analyzing the merits of electronic auctions. It uses that framework to evaluate the TFA case. The results of the analysis and the framework itself illustrate the various complex issues that arise in the design and implementation of electronic markets.
Bayesian Cognitive Modeling: A Practical Course (2014)

Bayesian inference has become a standard method of analysis in many fields of science. Students and researchers in experimental psychology and cognitive science, however, have failed to take full advantage of the new and exciting possibilities that the Bayesian approach affords. Ideal for teaching and self study, this book demonstrates how to do Bayesian modeling. Short, to-the-point chapters offer examples, exercises, and computer code (using WinBUGS or JAGS, and supported by Matlab and R), with additional support available online. No advance knowledge of statistics is required and, from the very start, readers are encouraged to apply and adjust Bayesian analyses by themselves. The book contains a series of chapters on parameter estimation and model selection, followed by detailed case studies from cognitive science. After working through this book, readers should be able to build their own Bayesian models, apply the models to their own data, and draw their own conclusions
Performance of a disk array protype (1991)

The RAID group at U.C. Berkeley recently built a prototype disk array. This paper examines the performance limits of each component of the array usiug SCSI bus traces, Sprite operating system traces and user programs.The array performs successfully for a workload of small, random I/O operations, achieving 275 I/Os per second on 14 disks before the Sun4/280 host becomes CPU-limited. The prototype is less successful in delivering high throughput for large, sequential operations. Memory system contention on the Sun4/280 host limits throughput to 2.3 MBytes/sec under the Sprite Operating System. Throughput is also limited by the bandwidth supported by the VME backplane, disk controller and disks, and overheads associated with the SCSI protocol.We conclude that merely using a powerful host CPU and many disks will not provide the full bandwidth possible from disk arrays. Host memory bandwidth and throughput of disk controllers are equally important. In addition, operating systems should avoid unnecessary copy and cache flush operations that can saturate the host memory system.