gebhart/patrepeval huggingface dataset:
Cardiopulmonary Complications of Intramedullary Fixation of Long Bone Metastases (2003)

Intramedullary fixation of long bone metastases is an effective method of treating or preventing pathologic fractures. An important complication of this technique is the development of pulmonary embolism, which may occur at any number of steps during bone manipulation. Passage of normal marrow contents or tumor into the pulmonary circulation is thought to cause various biochemical, hemodynamic, or physical responses that lead to hypotension, arrythmia, and O2 desaturation. Death is a known risk of this procedure. Numerous surgical and anesthetic strategies have been developed to prevent or treat pulmonary embolic phenomena; however, the most important prophylaxis may be a heightened awareness of this possibility during any procedure that involves intramedullary manipulation of tumor containing bone.
[Non-Hodgkin's lymphoma disclosed by an intrabronchial tumor. Apropos of a case]. (1991)

Non Hodgkin's lymphoma (LNH) presenting as a localised tumour is exceptional and nearly always appears during the course of disseminated disease. We report a case where the primary disease was an endobronchial tumour and the entire clinical picture related to pulmonary symptoms. A 70 year old lady was found to have a left sided pulmonary opacity following a cough with minimal expectoration and accompanied by chest pains and dyspnoea. The chest abnormality progressed for 3 years 9 months before an endobronchial tumour was discovered at bronchoscopy in the left upper lobe and from which a biopsy revealed an LNH with small cells of low degree of malignancy. In addition there was splenomegaly and an infiltration of bone marrow by the lymphomatous process which was evidence of a disseminated form of LNH. To our knowledge our observation is an extremely rare case where an endobronchial tumour revealed a non Hodgkins lymphoma.
High phosphatidylinositol 4-phosphate (PI4P)-dependent ATPase activity for the Drs2p-Cdc50p flippase after removal of its N- and C-terminal extensions (2017)

P4-ATPases, also known as phospholipid flippases, are responsible for creating and maintaining transbilayer lipid asymmetry in eukaryotic cell membranes. Here, we use limited proteolysis to investigate the role of the N and C termini in ATP hydrolysis and auto-inhibition of the yeast flippase Drs2p-Cdc50p. We show that limited proteolysis of the detergent-solubilized and purified yeast flippase may result in more than 1 order of magnitude increase of its ATPase activity, which remains dependent on phosphatidylinositol 4-phosphate (PI4P), a regulator of this lipid flippase, and specific to a phosphatidylserine substrate. Using thrombin as the protease, Cdc50p remains intact and in complex with Drs2p, which is cleaved at two positions, namely after Arg<sup>104</sup> and after Arg <sup>1290</sup>, resulting in a homogeneous sample lacking 104 and 65 residues from its N and C termini, respectively. Removal of the 1291-1302-amino acid region of the C-terminal extension is critical for relieving the auto-inhibition of full-length Drs2p, whereas the 1-104 N-terminal residues have an additional but more modest significance for activity. The present results therefore reveal that trimming off appropriate regions of the terminal extensions of Drs2p can greatly increase its ATPase activity in the presence of PI4P and demonstrate that relief of such auto-inhibition remains compatible with subsequent regulation by PI4P. These experiments suggest that activation of the Drs2p-Cdc50p flippase follows a multistep mechanism, with preliminary release of a number of constraints, possibly through the binding of regulatory proteins in the <i>trans</i>-Golgi network, followed by full activation by PI4P.
Satellite Conjunction Monte Carlo Analysis (2009)

This works uses a simplified Monte Carlo process to assess the satellite collision probability computations of various methods and to obtain a preliminary estimate on their bounds of utility. The process starts with a simple two-body analytical propagation of the position and velocity vectors and their associated 6x6 covariance from epoch to the time of closest approach (TCA). Each object is assumed spherical and a time span is set about the initial TCA to bound the results. No assumptions are made regarding the constancy or direction of the relative velocity over the encounter’s time span. The epoch vectors are randomly perturbed in accordance with the initial covariance matrix values and a collision determination made if the relative distance at any time in the span is equal to or less than the combined object radius. Two statistical bounding criteria are used to determine the minimum number of cases needed. The Monte Carlo results are then compared to probability computations that use the unperturbed orbital data at TCA. This particular work does not attempt to simulate real-world perturbations such as atmospheric drag and/or various gravity fields, but rather assures the consistency of the Gaussian process from epoch to closest approach point for the chosen force model. Because no unique or complicated force models are used the results can be easily repeated and independently validated.
κ Opioid Receptor Antagonism and Prodynorphin Gene Disruption Block Stress-Induced Behavioral Responses (2003)

Previous studies have demonstrated that stress may increase prodynorphin gene expression, and κ opioid agonists suppress drug reward. Therefore, we tested the hypothesis that stress-induced release of endogenous dynorphin may mediate behavioral responses to stress and oppose the rewarding effects of cocaine. C57Bl/6 mice subjected to repeated forced swim testing (FST) using a modified Porsolt procedure at 30°C showed a characteristic stress-induced immobility response and a stress-induced analgesia observed with a tail withdrawal latency assay. Pretreatment with the κ opioid receptor antagonist nor-binaltorphimine (nor-BNI; 10 mg/kg, i.p.) blocked the stress-induced analgesia and significantly reduced the stress-induced immobility. The nor-BNI sensitivity of the behavioral responses suggests an activation of the κ opioid receptor by a stress-induced release of dynorphin peptides. Supporting this hypothesis, transgenic mice possessing a disrupted prodynorphin gene showed no increase in immobility or stress-induced analgesia after exposure to repeated FST. Because both stress and the κ opioid system can modulate the response to drugs of abuse, we tested the effects of forced swim stress on cocaine-conditioned place preference (CPP). FST-exposed mice conditioned with cocaine (15 mg/kg, s.c.) showed significant potentiation of place preference for the drug-paired chamber over the responses of unstressed mice. Surprisingly, nor-BNI pretreatment blocked stress-induced potentiation of cocaine CPP. Consistent with this result, mice lacking the prodynorphin gene did not show a stress-induced potentiation of cocaine CPP, whereas wild-type littermates did. The findings suggest that chronic swim stress may activate the κ opioid system to produce analgesia, immobility, and potentiation of the acute rewarding properties of cocaine in C57Bl/6 mice.
Interaction of Anesthetics with the Rho GTPase Regulator Rho GDP Dissociation Inhibitor (2008)

The physiological effects of anesthetics have been ascribed to their interaction with hydrophobic sites within functionally relevant CNS proteins. Studies have shown that volatile anesthetics compete for luciferin binding to the hydrophobic substrate binding site within firefly luciferase and inhibit its activity (Franks, N. P., and Lieb, W. R. (1984) Nature 310, 599-601). To assess whether anesthetics also compete for ligand binding to a mammalian signal transduction protein, we investigated the interaction of the volatile anesthetic, halothane, with the Rho GDP dissociation inhibitor (RhoGDIalpha), which binds the geranylgeranyl moiety of GDP-bound Rho GTPases. Consistent with the existence of a discrete halothane binding site, the intrinsic tryptophan fluorescence of RhoGDIalpha was quenched by halothane (2-bromo-2-chloro-1,1,1-trifluoroethane) in a saturable, concentration-dependent manner. Bromine quenching of tryptophan fluorescence is short-range and W192 and W194 of the RhoGDIalpha are located within the geranylgeranyl binding pocket, suggesting that halothane binds within this region. Supporting this, N-acetyl-geranylgeranyl cysteine reversed tryptophan quenching by halothane. Short chain n-alcohols ( n < 6) also reversed tryptophan quenching, suggesting that RhoGDIalpha may also bind n-alkanols. Consistent with this, E193 was photolabeled by 3-azibutanol. This residue is located in the vicinity of, but outside, the geranylgeranyl chain binding pocket, suggesting that the alcohol binding site is distinct from that occupied by halothane. Supporting this, N-acetyl-geranylgeranyl cysteine enhanced E193 photolabeling by 3-azibutanol. Overall, the results suggest that halothane binds to a site within the geranylgeranyl chain binding pocket of RhoGDIalpha, whereas alcohols bind to a distal site that interacts allosterically with this pocket.
Nanorod–nanosheet hierarchically structured ZnO crystals on zinc foil as flexible photoanodes for dye-sensitized solar cells (2013)

In this paper, ZnO nanorod-nanosheet hierarchical structures were fabricated using a facile method on zinc foil and used as flexible photoanodes in dye-sensitized solar cells (DSCs). Compared to nanorods (NRs) obtained by the dissolution-precipitation method, the nanorod-nanosheet (NR-NS) hierarchical structures obtained by a second-step homogeneous precipitation improved the performance of DSCs by increasing photocurrent density significantly. As a result, the power conversion efficiency of the devices based on such a NR-NS hierarchical structure reached up to 2.4% under 100 mW cm(-2) illumination condition. This represents an enhancement by 108% as compared to DSCs based on NR assembled nanoflowers, for which the efficiency was 1.1%. The enhancement of the photocurrent was due mainly to the much larger specific surface area and resulting dye-loading amount. The electron transport properties in this structure were also investigated by means of electrochemical impedance spectroscopy (EIS). Furthermore, the formation mechanism of the NR-NS hierarchical structures are discussed.
A Classical Example of a Disappearing Polymorph and the Shortest Intermolecular H⋅⋅⋅H Separation Ever Found in an Organic Crystal Structure (2003)

Missing in action: Neutron and X-ray diffraction studies at low temperatures reveal the delicate balances of attractive and repulsive intermolecular interactions in the dimorphs of 1,2,3,5-tetra-O-acetyl-β-D-ribofuranose (the less stable polymorph is shown, O red, H blue, C gray). Very short intermolecular H⋅⋅⋅H contacts which are only present in the less stable crystal form are responsible for the disappearance of this polymorph. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2003/z19504_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Measurement of the evaporative cooling effect: oscillating misting fan (2017)

To determine the thermal effects of an oscillating mist fan (spraying 86 L/h of droplets with 25 µm mean diameter) on worker comfort, its effects on the thermal environment were measured in a large indoor space (37,500 m2/525,000 m3). It was found that the temperature dropped by 0.2–2.5 K, with local humidity increasing by 5%. Ventilation air-exchange calculations indicate that in hot summer conditions, an 8-h shift could be continuously cooled without creating high humidity, recovering to initial values after 16 h of ventilation at 0.3 ach. The cooling effect of the mist and fan was measured and compared with that of the fan. The mist and fan-cooling effect exceeded 100 W/m2 in all cases, while the mist and fan cooling exceeded the fan alone by 18 W/m2 on average, 24 W/m2 at the peak values and 11 W/m2 average during oscillation. The ASHRAE 55-2013 model is modified to include this additional cooling. Standard effective temperatures (SETs) are calculated with and without the mist fan-cooling effect. Linear approximations for reduction in SET were developed as a function of air temperature and mist-cooling effect. The deployment of this technology would improve thermal comfort for factory workers on hot summer days.
Synthesis and Characterization of Bioconjugates of S-Layer Proteins (2004)

The self-assembling proteins that form crystalline surface layers (S-layers) on many microbial species have found numerous applications due to their nanostructured nature. To devise a new method to construct surface displays that exploit S-layer self-assembly activity and nanostructural properties, we have constructed polymer bioconjugates of S-layer proteins. The conjugates formed are similar in function to the monomer alkanethiols that form self-assembled monolayers (SAMs) on gold surfaces. However, the self-assembly is driven by the protein "headgroup" that positions polymer-tethered endgroups on a surface. This paper examines the integration of protein purification, conjugation, and surface assembly that has led to the development of this new method for the formation of nanostructured surfaces. Purified S-layer proteins from Lactobacillus brevis were conjugated with small molecule probes and polymers using amine-based reactions. To keep multiple labeling of protein amine groups to acceptable levels, the conjugations were performed at pH 6.5, allowing for limited yields (24-39%) as determined by mass spectrometry and SDS-polyacrylamide gel electrophoresis. As the presence of high levels of unlabeled S-layer proteins is undesired, we have developed a protocol for further purification that employs monomeric avidin affinity chromatography. The surface self-assembly of the polymer bioconjugates onto amine-terminated microspheres was studied using epi-fluorescence, confocal, and scanning electron microscopy. The surfaces obtained exhibited homogeneous distributions of tethered molecules. Also, in cases where the modular assembly of two distinct types of tethered endgroups was accomplished, there was no evidence for phase separation in the surfaces. The modular assembly method will provide a potential route to controlling surface display density as the starting assembly conditions guide displayed endgroup concentrations in mixed molecular monolayers.
Gamma Endorphin and Hla Class I Related Immune Functions. Preliminary Observations (1990)

Click to increase image sizeClick to decrease image sizeKey Words: gamma-endorphinHLA class Iimmune functions
Prediction of Shoulder Dystocia Using Multivariate Analysis (2007)

We evaluated the use of multivariate analysis in the prediction of shoulder dystocia (SD). One hundred consecutive cases with SD were matched with 100 controls without dystocia. All patients had term, vaginal delivery. Multivariate analysis was used to identify independent variables significantly related to shoulder dystocia. The regression coefficients for the identified factors were used to calculate a composite score from which receiver operating characteristics (ROC) curves were derived. Birthweight (BW), 1-hour Glucola (GLU), operative vaginal delivery (OVD), and height of fundus (HOF) were related independently to SD. The sensitivity and specificity reached 84 and 80%, respectively, with BW + GLU + OVD. Significant associations persisted when HOF and carbohydrate intolerance were substituted for BW and GLU, respectively. SD is independently associated with BW, GLU, and OVD, and may be predicted with clinically acceptable accuracy using multiple variables. This model may be useful in the design of prospective studies for managing suspected macrosomia.
Hereditary Bullous Dystrophy, X-Linked (2011)

Abstract This chapter provides pictures and clinical details of X-LINKED HEREDITARY BULLOUS DYSTROPHY
A Novel CDX2 Isoform Regulates Alternative Splicing (2014)

Gene expression is a dynamic and coordinated process coupling transcription with pre-mRNA processing. This regulation enables tissue-specific transcription factors to induce expression of specific transcripts that are subsequently amplified by alternative splicing allowing for increased proteome complexity and functional diversity. The intestine-specific transcription factor CDX2 regulates development and maintenance of the intestinal epithelium by inducing expression of genes characteristic of the mature enterocyte phenotype. Here, sequence analysis of CDX2 mRNA from colonic mucosa-derived tissues revealed an alternatively spliced transcript (CDX2/AS) that encodes a protein with a truncated homeodomain and a novel carboxy-terminal domain enriched in serine and arginine residues (RS domain). CDX2 and CDX2/AS exhibited distinct nuclear expression patterns with minimal areas of co-localization. CDX2/AS did not activate the CDX2-dependent promoter of guanylyl cyclase C nor inhibit transcriptional activity of CDX2. Unlike CDX2, CDX2/AS co-localized with the putative splicing factors ASF/SF2 and SC35. CDX2/AS altered splicing patterns of CD44v5 and Tra2-β1 minigenes in Lovo colon cancer cells independent of CDX2 expression. These data demonstrate unique dual functions of the CDX2 gene enabling it to regulate gene expression through both transcription (CDX2) and pre-mRNA processing (CDX2/AS).
ChemInform Abstract: Second‐Generation Fluorous Chiral (Salen) Manganese Complexes. (2001)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Infrared radiation detector interrogated by Optical Frequency Domain Reflectometer (OFDR) (2011)

We experimentally demonstrated a fast infrared radiation sensor. The system is applicable in a quasi-distributed configuration to cover a large area using a single interrogation unit (OFDR) for early fire detection.
Scramjet/Ramjet Design and Integration Trade Studies Using SRHEAT (2009)

TM invaluable for initial assessment of hypersonic propulsion systems, providing the low cost analytical capability that makes rapid evaluation of design trade space possible. The ability to perform these trade studies as part of the early conceptual design phase is crucial to making correct initial design decisions before conducting more expensive preliminary and detail design efforts. These broad capabilities of SRHEAT TM are illustrated with a series of example trade studies, showing how this tool can be used to insure selection of the best combination of geometry, thermal and structural designs, sizing, vehicle integration, and mission flight path.
Nonlinear Response of a Dynamic System due to Oscillatory Flow (1987)

The dynamic response of a structure exhibiting nonlinear stiffness characteristics and excited by a nonzero mean oscillatory fluid flow is investigated. Both the method of averaging and a multi-frequency incremental harmonic balance approach are used in understanding the primary and secondary resonances in the response. Several parameter studies are presented where the results of these two methods are compared with those obtained by numerical integration and are discussed in detail. The results point to the need for a multi-frequency solution formulation for accurate representation of the response offset and to the difficulties of using the standard method of averaging formulation for investigation of secondary resonances in the response of this system.
Pharmacokinetics of procainamide in continuous ambulatory peritoneal dialysis. (1988)

Procainamide (PA) and N-acetylprocainamide (NAPA) pharmacokinetics were examined in six patients undergoing continuous ambulatory peritoneal dialysis (CAPD) following a single oral dose of 625 mg of procainamide HCl. Peak serum PA concentrations occurred between 1 and 3 h after drug administration whereas peak NAPA levels were reached 14 to 48 h following the administration of PA. NAPA levels 1.2 mcg/ml or greater were present for 72 h and in four of the six subjects little further decline occurred at 96 h. PA total body clearance (TBC) averaged 143 ml/min; TBC for NAPA was 29.8 ml/min. PA and NAPA half-lives were considerably prolonged averaging 26.0 and 42.8 h, respectively. V beta for PA was 4.1 l/kg and for NAPA was 1.46 l/kg. PA and NAPA dialysance varied from 0.28 to 5.55 ml/min and from 1.74 to 7.20 ml/min, respectively. This, in turn, represented less than 5 and 25% of the TBC for each drug.
expression in Escherichia coli of a recombinant fragment (He 914-Leu 1364) of human von Willebrand factor containing a collagen binding domain (1990)

Previous studies have shown that a collagen binding domain of human von Willebrand factor (vWF) resides on a fragment named SpI obtained by digestion with Staphylococcus aureus V8 protease which corresponds to residues Gly 911-Glu 1365 of the mature plasma vWF subunit. We have subcloned a fragment of a full-length cDNA encoding vWF into an expression vector which uses an inducible lambda PL promoter. The predicted product expressed by this plasmid is a fusion protein consisting of 16 amino acids (aa) of the lambda cII protein and aa Ile 914-Leu 1364 of human vWF. This fusion protein was shown to be expressed as insoluble inclusion bodies by induced E. coli harbouring the recombinant vector and was partially purified from bacterial debris and renatured. Partially purified bacterial extract run on SDS-polyacrylamide gels contained a major band representing 50-70% of the visualized proteins and corresponded to the predicted fusion protein. This band reacted with both polyclonal antibodies against human vWF and monoclonal antibodies (MAbs) which recognize the SpI fragment of plasma vWF. The radiolabelled partially purified bacterial extract was shown to bind specifically to human fibrillar collagen types I and III. This binding, which was a function of radiolabelled ligand and collagen concentrations, did not occur on monomeric denatured collagen. It was inhibited by both unlabelled bacterial extract and plasma vWF and also by a MAb against vWF SpI, which has the property of inhibiting vWF/collagen interaction. Our data demonstrate that this recombinant vWF SpI fragment, which can be obtained in large amounts, is a useful model for localizing the epitopes of monoclonal antibodies and then for studying the mechanism of vWF/collagen interaction.
Acceleration of photodarkening measurements in Yb-doped fibers by enhanced temperatures (2009)

The probe transmission at 633 nm was plotted during core pumping with 300 mW (pump wavelength 976 nm) what established an inversion of about 0.47 in the short fiber pieces of some centimeters. The results are discussed also in comparison to measurements at room temperature in dependence on the inversion, and the suitability of the suggested method of PD characterization at enhanced temperatures is evaluated for fibers with low Yb doping.
Synergism of composition of nitrogen- and sulfur-containing compounds as a tribological active additive to lubricants (2013)

A composition containing ammonium salt of alkyldithiophosphoric acid with tetra(2-ethylhexyl)thiuramdisulfide is proposed as an effective tribological additive for lubricants. In addition, it provides a lower amount of sulfate ash, phosphorus, and sulfur in the motor oil. The composition demonstrates synergism if a fixed atomic ratio of sulfur to phosphorus is observed.