gebhart/patrepeval huggingface dataset:
Honokiol inhibits signal transducer and activator of transcription‐3 signaling, proliferation, and survival of hepatocellular carcinoma cells via the protein tyrosine phosphatase SHP‐1 (2011)

The activation of signal transducers and activators of transcription 3 (STAT3) has been closely linked with the proliferation, survival, invasion, and angiogenesis of hepatocellular carcinoma (HCC) and represents an attractive target for therapy. In the present report, we investigated whether honokiol mediates its effect through interference with the STAT3 activation pathway. The effect of honokiol on STAT3 activation, associated protein kinases, and phosphatase, STAT3-regulated gene products and apoptosis was investigated using both functional proteomics tumor pathway technology platform and different HCC cell lines. We found that honokiol inhibited both constitutive and inducible STAT3 activation in a dose- and time-dependent manner in HCC cells. The suppression was mediated through the inhibition of activation of upstream kinases c-Src, Janus-activated kinase 1, and Janus-activated kinase 2. Vanadate treatment reversed honokiol-induced down-regulation of STAT3, suggesting the involvement of a tyrosine phosphatase. Indeed, we found that honokiol induced the expression of tyrosine phosphatase SHP-1 that correlated with the down-regulation of constitutive STAT3 activation. Moreover, deletion of SHP-1 gene by siRNA abolished the ability of honokiol to inhibit STAT3 activation. The inhibition of STAT3 activation by honokiol led to the suppression of various gene products involved in proliferation, survival, and angiogenesis. Finally, honokiol inhibited proliferation and significantly potentiated the apoptotic effects of paclitaxel and doxorubicin in HCC cells. Overall, the results suggest that honokiol is a novel blocker of STAT3 activation and may have a great potential for the treatment of HCC and other cancers.
A new salmonella serotype, Salmonella bury (4, 27, 12 : c - z6) of human origin (1956)

Microbiology Society journals contain high-quality research papers and topical review articles. We are a not-for-profit publisher and we support and invest in the microbiology community, to the benefit of everyone. This supports our principal goal to develop, expand and strengthen the networks available to our members so that they can generate new knowledge about microbes and ensure that it is shared with other communities.
Na<sup>+</sup>-K<sup>+</sup>ATPase distribution in frog and bovine lenses (1985)

Na+-K+ ATPase activity was measured in the capsule-epithelium and decapsulated frog and bovine lens. The decapsulated lens contained approximately 20% of the whole lens activity in the frog and 30% in the bovine. These values were measured from the aqueous homogenate of the entire decapsulated lens, an approach which may have underestimated the activity by diluting the ouabain-sensitive component in the preparation. Subsequent determinations were done on separate portions of superficial (2 to 3 mm) anterior-equatorial, and posterior bovine cortex. The activities per gram of tissue were enriched with respect to the values for the entire decapsulated bovine lens. These activities were further enriched by a sucrose density gradient centrifugation. The anterior-equatorial cortical segment contained 1.6 times the activity found in the capsule-epithelium. The posterior cortex had a much smaller but statistically significant level of Na+-K+ ATPase. It is unlikely that the observed asymmetry of the anterior-equatorial segment with the posterior cortex is exclusively due to epithelial contamination for the result would require the adherence of 62% of the epithelium. Scanning electron micrographs of 6 decapsulated bovine lenses indicated an average contamination of about 9%. This asymmetry may have a physiological role in assisting the pump mechanism of the epithelium.
Gamma -X crossover in GaAs/AlAs short period superlattice under pressure (1991)

Photoluminescence (PL) spectra under pressure have been measured at 80 K for MBE grown (GaAS)m(AlAs)m superlattices (SLS). Two main emission bands have been observed for the (GaAs)12(AlAs)12SL. The higher emission band (1.785 eV) behaves like a Gamma edge under pressure and the lower emission band (1.749 eV), on the other hand, behaves like an X edge. One emission band at 1.664 eV has been observed for (GaAs)18(AlAs)18SL at atmospheric pressure. This band moves to higher energy when pressure is applied, which shows the lowest lying conduction band is Gamma like. The PL emission intensity of this SL decreases abruptly to the order of 10-2 at 7.5 kbar, which is due to the pressure-induced Gamma -X crossover. The pressure-induced change-over from Gamma - to X-like emission has been clearly observed for (GaAs)15(AlAs)15SL. These results give conclusive evidence to verify the finding that, on increasing the number of monolayers, the lowest conduction band edge of (GaAs)m(AlAs)m switches from the X-like to Gamma -like state at a thickness of 14 monolayers.
Non-Invasive Monitoring of Urinary KRAS Circulating Tumor DNA for Treatment Response and Minimal Residual Disease in Patients with Lung Adenocarcinoma. (2015)

e19092 Background: Radiographic imaging in lung adenocarcinoma patients is not always reliable and a non-tissue based approach for monitoring treatment responses or minimal residual disease is desired. 15–25% of patients with lung adenocarcinoma have tumor associated KRAS mutations, and these may be detected in circulating tumor (ct) DNA. The aim of this study was to correlate the dynamics of KRAS mutational load in ctDNA with clinical responses in patients with lung adenocarcinoma receiving surgical treatment, radiation, or chemotherapy. Methods: In a blinded biomarker study of 30 patients, urine samples were obtained from 7 patients with metastatic or early stage lung adenocarcinoma harboring KRAS G12/13 mutation in the tumor tissue by CLIA laboratory testing. Following extraction of urinary ctDNA, KRAS G12/13 mutational load was quantified using a next generation sequencing-based method with standardized reporting of mutant copies per 105genome equivalents. Results: Of 7 patients enrolled, 6 patients with lung adenocarcinoma had serial urine collections with up to 3 specimens at 1-3 month intervals. Three of 7 patients had metastatic disease receiving chemotherapy, and 2 of 3 had serial detectable KRAS mutation in urinary ctDNA which tested positive 1 to 2 months prior to radiologic progression. In 3 of 4 patients with definitive stereotactic body radiation therapy (SBRT) or surgery for early stage cancer, and now with no clinical evidence of disease, mutant KRAS was not detectable in urinary ctDNA at any time points after surgery. In 1 of 4 patients with early stage disease, imaging was suspicious for progression, and this presentation correlated with an increase in urinary ctDNA KRAS mutation load after surgery. Conclusions: We demonstrate feasibility of monitoring minimal residual disease and responses to chemotherapy in patients with KRAS G12/13-positive lung adenocarcinoma using a completely non-invasive urine sample.
Simulation of the femtosecond optical response of a solute in water (1992)

We present microscopic semiclassical simulations of the femtosecond nonlinear-optical polarization (${\mathit{P}}^{(3)}$) of a solute in water. ${\mathit{P}}^{(3)}$ is dominated at short times by intermolecular vibrations that cannot be accounted for by the Bloch equations. The applicability of the Bloch, stochastic, and Brownian oscillator models to the interpretation of photon-echo and pump-probe measurements is discussed. We find that only microscopic semiclassical simulation and the Brownian oscillator model can account for the essential features of ${\mathit{P}}^{(3)}$.
Stereo Image-Based Arm Tracking for In Vivo Surgical Robotics (2011)

Motor-based tracking and image-based tracking are considered for three-dimensional in vivo tracking of the arms of a surgical robot during minimally invasive surgery. Accurate tracking is necessary for tele-medical applications and for the future automation of surgical procedures. An experiment is performed to compare the accuracy of the two methods, and results show that the positioning error of image-based tracking is significantly less than that of motor-based tracking.
Crystallinity in jute fiber as revealed by multipeak resolution (1977)

Abstract A method of multipeak resolution and computation of background scatter in x‐ray diffraction from fiber has been applied to jute fibers. While crystallinity as determined by this method in raw and delignified jute fibers remains unchanged, perfection of the crystalline regions improves on delignification.
Control of Blood Glucose in Diabetics using an Artificial Pancreas* (1977)

Studies have been performed using an on-line computer system programmed for blood gucose control of insulin and dextrose infusion (artificial pancreas). The aim of these studies was to test performance of the artificial pancreas and to suggest directions for future optimisation. Blood glucose stabilisation studies of diabetic volunteers were extended throughout the day and included three main meals and light exercise periods. Monitoring of blood glucose profiles of the same diabetics after depot insulin were performed on a separate occasion for comparison. The presence of insulin antibodies did not impair operation of the artificial pancreas. Most of the insulin infused by the artificial pancreas was to initially correct hyperglycaemia with relatively little required to subsequently maintian euglycaemia. The afternoon intra-meal average infusion rate was 0-9 U/hr. It is suggested that correction of fasting hyperglycaemia and maintenance of euglycaemia in diabetics be treated as separate control problems for the artificial pancreas. The overall ability of the artificial pancreas to control blood glucose to a degree not attainable by conventional insulin therapy is confirmed, in this case under conditions which include patient activity.
ChemInform Abstract: Microwave‐Assisted Multicomponent Reactions in the Synthesis of Heterocycles (2013)

Abstract Review: 237 refs.
Optimal Cluster Head Spacing for Energy-Efficient Communication in Aerial-Backhauled Networks (2015)

In this paper we introduce a novel analytic approach in clustering wireless nodes on ground fields through the assistance of an aerial base station, forming an aerial backhauled network. On the ground, some of the deployed nodes act as cluster heads, and aggregate and relay the terrestrial data towards the aerial platform. We present a novel cluster-head selection algorithm based on Matern hard-core point process, and then obtain the analytical formulation to optimize the spacing between cluster heads to minimize the overall energy consumption. The formulated problem utilizes stochastic geometry to capture the random nature of the locations of the deployed nodes, leading to a tractable analysis of the expected network metrics. Furthermore, we compare the performance of the proposed approach with other clustering algorithms, and show the improvement in energy efficiency.
Synthetic THz Nanoholography for Imaging CVD Graphene (2018)

We combine THz scattering-type scanning near-field optical microscopy (s-SNOM) and synthetic optical holography (SOH) to enable fast, amplitude and phase resolved THz nanoimaging using a THz gas laser and Schottky diode detector. We demonstrate our novel tool by imaging graphene grown via chemical vapor deposition (CVD) and other 2D materials.
Contribution of Lyb 5+ and Lyb 5- B cells to the primary and secondary phosphocholine-specific antibody response. (1983)

Due to a mutation on their X-chromosome, CBA/N mice lack the Lyb-5+ subset of B cells. The loss of this B cell subset results in a profound alteration in the immune response of these mice to the hapten phosphocholine (PC). Thus, when these mice are immunized with high doses of PC-KLH (200 micrograms) in CFA, they: 1) fail to produce IgM anti-PC antibodies; 2) produce little or no anti-PC antibody bearing the normally predominant T15-idiotype; and 3) produce IgG anti-PC antibodies only late in the primary response. In order to more fully delineate this defect in responsiveness to PC, the splenic focus assay was used to analyze Lyb-5- B cell precursors from both normal and immune defective mice. Lyb-5- cells were obtained from normal (CBA/N x DBA/2)F1 (CD) female spleens by treatment with anti-Lyb-5 serum and complement. These normal Lyb-5- cells and Lyb-5- cells from immune defective CD male mice were stimulated in vitro with either PC-Hy or TNP-Hy in the presence of Hy-primed T helper cells. The results demonstrate that primary Lyb-5- PC-specific B cells fail to respond in the splenic focus assay, while secondary Lyb-5- PC-specific precursors respond normally, and that both primary and secondary Lyb-5- TNP-specific precursors respond in the splenic focus assay. These data suggest that Lyb-5- PC-specific precursors must differentiate into memory cells before they can be activated to secrete antibody, and they also indicate that the Lyb-5- B cell subset may be composed of two subsets with different activation requirements.
Viscosity and tracer diffusion coefficients, and thermodynamic properties, for ethanol + acetonitrile mixtures at 298.15 K (1983)

Molar volumes and partial molar volumes, tracer diffusion coefficients (D), and shear viscosities (?) are reported for ethanol + acetonitrile mixtures at 298.15 K. The tracer diffusion coefficients vary non-additively with composition, and the product D? for each component is composition-dependent. Albright's equation for viscosity reproduces experimental viscosities to within the combined uncertainties of the experimental data. The system exhibits considerably less complex behaviour than does the analogous water + acetonitrile system, and the properties are thought to be dominated by dipole-dipole interactions between unlike molecules.
Temperature sweep gel electrophoresis: a simple method to detect point mutations (1991)

Journal Article Temperature sweep gel electrophoresis: a simple method to detect point mutations Get access Kenji Yoshino, Kenji Yoshino Department of Environmental Chemistry, Saitama UniversityUrawa 338, Japan Search for other works by this author on: Oxford Academic PubMed Google Scholar Koichi Nishigaki, Koichi Nishigaki Department of Environmental Chemistry, Saitama UniversityUrawa 338, Japan Search for other works by this author on: Oxford Academic PubMed Google Scholar Yuzuru Husimi Yuzuru Husimi * Department of Environmental Chemistry, Saitama UniversityUrawa 338, Japan * To whom correspondence should be addressed Search for other works by this author on: Oxford Academic PubMed Google Scholar Nucleic Acids Research, Volume 19, Issue 11, 11 June 1991, Page 3153, https://doi.org/10.1093/nar/19.11.3153 Published: 11 June 1991 Article history Received: 05 April 1991 Published: 11 June 1991
Long‐term evaluation of degradation and foreign‐body reaction of subcutaneously implanted poly(DL‐lactide‐ε‐caprolactone) (1997)

The aim of this study was to evaluate the degradation and foreign-body reaction of poly(DL-lactide-ϵ-caprolactone) (PLA85CL50) bars. This specific biomaterial is used for the construction of nerve guides, which can be used in the reconstruction of short nerve gaps. Subcutaneously implanted PLA85CL50 bars were harvested after implantation periods ranging from 3 to 12 months and evaluated for the rate of degradation and the degree of foreign-body reaction. It was observed that this copolymer degraded completely within 12 months and that no lactide or ϵ-caprolactone crystals were formed. Furthermore, we conclude that the foreign-body reaction of PLA85CL50 is very mild. These properties make the amorphous copolymer of DL-lactide and ϵ-caprolactone (50:50) suitable for the construction of nerve guides. © 1997 John Wiley & Sons, Inc. J Biomed Mater Res, 36, 337–346, 1997.
Chromatin domain activation via GATA-1 utilization of a small subset of dispersed GATA motifs within a broad chromosomal region (2005)

Cis elements that mediate transcription factor binding are abundant within genomes, but the rules governing occupancy of such motifs in chromatin are not understood. The transcription factor GATA-1 that regulates red blood cell development binds with high affinity to GATA motifs, and initial studies suggest that these motifs are often unavailable for occupancy in chromatin. Whereas GATA-2 regulates the differentiation of all blood cell lineages via GATA motif binding, the specificity of GATA-2 chromatin occupancy has not been studied. We found that conditionally active GATA-1 (ER-GATA-1) and GATA-2 occupy only a small subset of the conserved GATA motifs within the murine beta-globin locus. Kinetic analyses in GATA-1-null cells indicated that ER-GATA-1 preferentially occupied GATA motifs at the locus control region (LCR), in which chromatin accessibility is largely GATA-1-independent. Subsequently, ER-GATA-1 increased promoter accessibility and occupied the betamajor promoter. ER-GATA-1 increased erythroid Krüppel-like factor and SWI/SNF chromatin remodeling complex occupancy at restricted LCR sites. These studies revealed three phases of beta-globin locus activation: GATA-1-independent establishment of specific chromatin structure features, GATA-1-dependent LCR complex assembly, and GATA-1-dependent promoter complex assembly. The differential utilization of dispersed GATA motifs therefore establishes spatial/temporal regulation and underlies the multistep activation mechanism.
Universal valence bands for rocksalt-type compounds and their connection with those of tetrahedral crystals (1975)

Using a limited tight-binding basis set, we obtain a universal set of analytical valence bands for rocksalt-type compounds and the rare-gas solids. The bands for each material are obtained by multiplying the universal bands by a scaling factor which is a simple function of the lattice constant and the chemical valence $Z$ of the constituent atoms. Thus, in terms of only two empirically-determined parameters, we get complete valence bands for 51 crystals. We also obtain a universal density-of-states curve in terms of which photoemission spectra may be directly interpreted. Finally, we give a detailed comparison of the valence bands of rocksalt-type compounds with those of the zinc-blende-type compounds and show that the latter are a simple modification of the former. The only fundamental change is the introduction of a splitting at $W$, and along $Z$ and $\ensuremath{\Sigma}$, which is a manifestation of the tetrahedral forces that are present in the zinc-blende but not the rocksalt crystals. This result complements the well-known fact that the zinc-blende bands are a simple modification of diamond-type bands.
Tunable CW laser operation of Tm <sup>3+</sup> in locally disordered NaLa(WO <sub>4</sub> ) <sub>2</sub> (2007)

We report now on lasing of Tm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3+</sup> in the isostructural host NaLa(WO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">4</sub> ) <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> , shortly NaLaW, which was grown for the present work by the Czochralski method, with 5 mol % Tm substitution in the melt.
Pulsed Hydrogen Exchange and Electrospray Charge-State Distribution as Complementary Probes of Protein Structure in Kinetic Experiments:  Implications for Ubiquitin Folding (2005)

The possible involvement of "hidden" kinetic intermediates in the apparent two-state folding of some proteins is currently a matter of debate. This study uses time-resolved electrospray ionization (ESI) mass spectrometry with on-line pulsed hydrogen-deuterium exchange (HDX) for monitoring the refolding of acid/methanol-denatured ubiquitin. It is demonstrated that the ESI charge-state distribution (CSD) and the extent of HDX represent nonredundant probes of the protein structure in solution. When considered in isolation, the data provided by both of these probes are consistent with a two-state behavior, involving only denatured ubiquitin D and refolded protein F. However, a careful comparison of the CSD and HDX kinetics reveals the presence of an additional species, exhibiting a CSD like the folded protein but showing non-native HDX characteristics. This kinetic intermediate, D*, is in rapid equilibrium with D, such that the overall reaction is consistent with the mechanism D <--> D* --> F. The results of this work suggest that the occurrence of transient intermediates may be more widespread than commonly thought, especially in cases where a cursory analysis indicates two-state behavior.
Indirect DRM Evaluation Architecture (2007)

In this paper we consider some of the basic design principles that should support the development of future DRM systems. We begin by discussing some of the problems associated with current DRM environments, and how they might be alleviated through the adoption of an open architectural framework based on basic design principles. We then demonstrate a particular implementation referred to as the IDEA architecture.
Structure of the red fluorescent protein from a lancelet (<i>Branchiostoma lanceolatum</i>): a novel GYG chromophore covalently bound to a nearby tyrosine (2013)

A key property of proteins of the green fluorescent protein (GFP) family is their ability to form a chromophore group by post-translational modifications of internal amino acids, e.g. Ser65-Tyr66-Gly67 in GFP from the jellyfish Aequorea victoria (Cnidaria). Numerous structural studies have demonstrated that the green GFP-like chromophore represents the `core' structure, which can be extended in red-shifted proteins owing to modifications of the protein backbone at the first chromophore-forming position. Here, the three-dimensional structures of green laGFP (λex/λem = 502/511 nm) and red laRFP (λex/λem ≃ 521/592 nm), which are fluorescent proteins (FPs) from the lancelet Branchiostoma lanceolatum (Chordata), were determined together with the structure of a red variant laRFP-ΔS83 (deletion of Ser83) with improved folding. Lancelet FPs are evolutionarily distant and share only ∼20% sequence identity with cnidarian FPs, which have been extensively characterized and widely used as genetically encoded probes. The structure of red-emitting laRFP revealed three exceptional features that have not been observed in wild-type fluorescent proteins from Cnidaria reported to date: (i) an unusual chromophore-forming sequence Gly58-Tyr59-Gly60, (ii) the presence of Gln211 at the position of the conserved catalytic Glu (Glu222 in Aequorea GFP), which proved to be crucial for chromophore formation, and (iii) the absence of modifications typical of known red chromophores and the presence of an extremely unusual covalent bond between the Tyr59 C(β) atom and the hydroxyl of the proximal Tyr62. The impact of this covalent bond on the red emission and the large Stokes shift (∼70 nm) of laRFP was verified by extensive structure-based site-directed mutagenesis.
EFFECT OF HYPOTENSION INDUCED BY SODIUM NITROCYANOFERRATE (III) ON THE RELEASE OF ARGININE-VASOPRESSIN IN THE UNANAESTHETIZED MONKEY (1978)

*I.N.S.E.R.M., U. 176, Laboratoire de Neurophysiologie, Université de Bordeaux II, F. 33076 Bordeaux Cedex, France and †I.N.S.E.R.M., U. 30, Hôpital des Enfants Malades, 149 rue de Sèvres, F. 75015 Paris, France ( Received 17 March 1978) Haemorrhage is a potent stimulus for the liberation of arginine-vasopressin (AVP) in the unanaesthetized monkey (Arnauld, Czernichow, Fumoux & Vincent, 1977), but in contrast to results obtained with other species, the fall in arterial blood pressure seems to be a more important factor than the reduction in blood volume. However, hypotension induced by haemorrhage varies considerably from one experiment to another, irrespective of the amount of blood withdrawn. In order to control changes in arterial blood pressure more accurately and to study their effect on the release of AVP in the conscious monkey, it was decided to use infusions of sodium nitrocyanoferrate (III), a drug which is known to induce a sustained fall in arterial