Experimental evaluation of input-output models of motoneuron discharge (1996)

1. We measured the modulation of the background firing rate of cat spinal motoneurons produced by simulated, repetitive excitatory postsynaptic potentials (EPSPs) to test the accuracy of several proposed motoneuron input-output functions. Rhythmic discharge was elicited in the motoneurons by injecting suprathreshold current steps 1-1.5 s in duration. On alternate trials, trains of short (0.5-5 ms) current pulses were superimposed on the current steps to stimulate the effects of trains of individual EPSPs. The increase in firing rate (delta F) due to the addition of the pulses was calculated as the difference in motoneuron discharge rate between trials with and without the superimposed pulse trains. 2. In the same motoneurons, we were able to study the effects of changes in pulse frequency, duration, and amplitude, as well as changes in the background discharge rate. A sublinear relationship between pulse rate and delta F was observed, with delta F rising relatively steeply with increasing pulse frequency at low pulse rates and saturating at high pulse rates. A similarly shaped relation was observed between delta F and pulse duration. In contrast, delta F generally increased in a greater than linear fashion with increasing pulse amplitude. 3. In previous studies we demonstrated that when a relatively constant synaptic input is produced by high-frequency synaptic activity, delta F is approximately equal to the product of the net synaptic current reaching the soma and the slope of the motoneuron's steady-state frequency-current (f-I) relation. In the present study, this input-output function consistently underestimated the observed delta F, particularly for low input rates, indicating that the transient current pulses are more effective in modulating motoneuron discharge than an equivalent amount of constant current. 4. Other investigators have proposed input-output functions derived from the relation between synaptic potential amplitude and the magnitude of the peak of a cross correlogram compiled from the discharge of the pre- and postsynaptic neurons. These functions consistently overestimated the observed delta F, particularly for high pulse rates. This overestimation may result in part from the fact that the effects of a synaptic potential (or current pulse) on postsynaptic discharge probability also include a period of decreased firing probability. Moreover, the cross correlation function may depend on the arrival rate of synaptic potentials (or current pulses). 5. Another proposed input-output function based on a simple threshold-crossing model of the motoneuron with a fixed spike threshold predicts firing rates that were often close to the observed delta F. However, the model did not reproduce the observed relations between delta F and input pulse rate or pulse duration. 6. The deficiencies of the basic threshold-crossing model may arise from the fact that it does not incorporate variations in membrane conductance and firing threshold that occur in real motoneurons. A more complete motoneuron model that incorporates both of these features was able to replicate the observed delta Fs associated with changes in input pulse frequency and duration.