851 EARLY DEVELOPMENT OF A MULTIPLEX GIANT MAGNETORESISTIVE NANOSENSOR TO DETECT URINARY MARKERS TO DISCRIMINATE BETWEEN HIGH AND LOW GRADE PROSTATE CANCER (2011)

You have accessJournal of UrologyProstate Cancer: Detection and Screening1 Apr 2011851 EARLY DEVELOPMENT OF A MULTIPLEX GIANT MAGNETORESISTIVE NANOSENSOR TO DETECT URINARY MARKERS TO DISCRIMINATE BETWEEN HIGH AND LOW GRADE PROSTATE CANCER Yuanpeng Li, Balasubramanian Srinivasan, Jianping Wang, Chengguo Xing, and Joel Slaton Yuanpeng LiYuanpeng Li Minneapolis, MN More articles by this author , Balasubramanian SrinivasanBalasubramanian Srinivasan Minneapolis, MN More articles by this author , Jianping WangJianping Wang Minneapolis, MN More articles by this author , Chengguo XingChengguo Xing Minneapolis, MN More articles by this author , and Joel SlatonJoel Slaton Minneapolis, MN More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2011.02.672AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES A series of urinary biomarkers have recently been identified in single institutional studies that perform superior to PSA in identifying patients with prostate cancer; two of these markers were also helpful in distinguishing between low and high grade cancer. We have developed a novel giant magnetoresistive (GMR) nanosensor which can be functionalized with antibodies and can identify protein ligands directly from body fluids. We have made progress in functionalizing this nanosenor to identify these urinary protein prostate cancer biomarkers directly from a fresh sample of urine in order to discriminate between low and high grade cancer. METHODS Our GMR sensor integrating magnetic moment Fe-Co nanoparticles minimizes the detecting distance between the nanoparticles and the GMR sensing. The two-layer competition assay was used first to detect endoglin (a recently identified urinary marker for prostate cancer) from the urine (4μL) where urinary endoglin competes with high magnetic moment FeCo nanoparticle labeled endoglin for capture antibody on the GMR sensor surface. Nanoparticle labeled endoglin in PBS buffer binding to capture antibody on the sensor surface served as a control. Pre-biopsy urine from 5 patients with no cancer on biopsy, 5 with Gleason grade (3+3) cancer, and 5 from patients with Gleason >8 cancer were tested for ability of the functionalized endoglin nanosensor to discriminate patients. RESULTS Using urine spiked for endoglin, we detected concentrations of endoglin as low as 1x10–19 mole. Our results show that the sensing signals obtained from buffer and endoglin depleted urine do not statistically differ from each other. Urine test results from the three-layer competition assay were fitted into the dose-response curve to deduce endoglin levels in the urine samples. Results show that endoglin concentration levels are significantly different between high grade (208±123 fM), low grade prostate cancer (64.5±60.3 fM) patients and no prostate cancer patient's urines (29.7±31.5 fM). CONCLUSIONS Our device has strong specificity for low-level urinary biomarkers. Furthermore, our endoglin functionalized device was able to easily discriminate between urine from patients with high grade cancer low grade cancer, and no cancer using less than 4 ul of urine. We are currently functionalizing the GMR nanosenor in order to detect four other urinary biomarkers to create a multiplexed detection device. © 2011 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 185Issue 4SApril 2011Page: e342 Advertisement Copyright & Permissions© 2011 by American Urological Association Education and Research, Inc.MetricsAuthor Information Yuanpeng Li Minneapolis, MN More articles by this author Balasubramanian Srinivasan Minneapolis, MN More articles by this author Jianping Wang Minneapolis, MN More articles by this author Chengguo Xing Minneapolis, MN More articles by this author Joel Slaton Minneapolis, MN More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...