gebhart/patrepeval huggingface dataset:
Drug Selection Enrichment of Type II Pneumocytes Following Transplant of P140K MGMT Lentiviral Transduced Hematopoietic Stem Cells. (2004)

Abstract Recent reports have shown that bone marrow derived cells can differentiate into epithelial cells of the lung. Data indicate that this event occurs in the absence of cell fusion, however the numbers of donor-derived cells are present at a low frequency. In this study, we investigated whether hematopoietic donor-derived Type II pneumocytes could be enriched by lentiviral or retroviral expression of P140K MGMT. MGMT encodes O6 alkylguanine DNA alkyltransferase (AGT), a major drug resistance protein for DNA damage induced by BCNU. This gene transfer approach provides a selective advantage to wild type donor cells by transduction with a mutant human AGT (P140K MFG-MGMT) which confers resistance to BG and BCNU. P140K transduced whole bone marrow or Sca+kit+lin- cells were transplanted into lethally irradiated wild type recipients followed by two or three doses of 30 mg/kg BG and 7.5–10 mg/kg BCNU. After drug selection, human AGT expressing murine cells comprised up to 99% of bone marrow in mice harvested 5–7 months after transplant (n=8). These mice also showed enrichment for the total number of donor derived alveolar cells after drug treatment from 25% (n= 2 untreated mice) to 46% ± 9.7 (n=4 mice; p=.0001). The high percentage of donor cells was attributed to the hematopoietic cells present in the lung, however there were also donor-derived cells with epithelial morphology. Similar bone marrow plasticity studies have elicited controversy, largely due to the technical limitations of in situ detection of organ specific donor cells. To overcome the inability to accurately detect single cells in lung tissue, we used dispase mediated cell dissociation followed by analysis of single cell suspensions by fluorescent microscopy. Type II pneumocytes were detected using a Pro-surfactant protein C antibody that is specific for alveolar Type II cells. Donor P140K transduced cells were co-detected using an antibody to AGT. Using this method, 10–12% of total cells isolated from the lung were Type II pneumocytes and 1.5–8% of these cells co-expressed AGT in mice treated with BG and BCNU (n=3). This indicated drug selection mediated expansion of transplanted marrow-derived cells with characteristics of lung epithelial Type II pneumocytes. These numbers represent an increase compared to untreated mice in which we were unable to detect donor-derived Type II cells (n=2) and published results indicating 0.2–0.6% of total lung cells are bone marrow-derived epithelial cells. This study indicates that MGMT mediated drug selection of hematopoietic cells, using lentiviral MGMT gene transfer, enhances their capacity for organ specific selection and repopulation of lung alveolar epithelial cells. Drug selection gene transfer may augment transdifferentiation and have therapeutic implications for pulmonary disorders.
ChemInform Abstract: RK. VON 2‐ARYLSULFONYLOXY‐TROPONEN MIT AKTIVEN METHYLENVERBINDUNGEN, BLDG. VON 8‐HYDROXY‐2H‐CYCLOHEPTA(B)FURANON‐(2)‐ UND 2‐AMINO‐8H‐CYCLOHEPTA(B)FURANON‐(8)‐DERIVATEN (1972)

Abstract Die Reaktion der Tropone (I) mit den Estern (II) in Äthanol in Gegenwart von Na‐äthylat (NAT) bei 0°C führt zu den Furanonen (III) und (IV), und zwar entsteht mit (IIa) als Reaktionspartner als Hauptprodukt das Hydroxy‐Derivat (IVa) und daneben nur sehr wenig (IIIa), mit (IIb) dagegen das Cycloheptafuranon (IIIb) vorrangig vor dem Alkohol (IVb).
A multiplex immunoassay of serum biomarkers for the detection of uveal melanoma (2019)

The multiplex immunoassay provides sufficient analytical performance to evaluate serum biomarkers that complement each other in detection of UM, and warrants further validation with a larger number of patient samples.
Prescribing exercise for health: a simple framework for primary care. (1996)

Physicians need to become committed to promoting the preventive benefits of regular physical activity. Practical strategies can help physicians efficiently and effectively incorporate exercise counseling into their practices. These strategies include regularly asking about leisure activity and linking the agenda of an office visit to the benefits of exercise. The physician and the patient should work together to formulate a mutually acceptable plan for the patient to adopt and maintain a healthful exercise lifestyle. In the same way that prescriptions are written for medications, a prescription can be given for an exercise plan suited to the individual patient.
Soil erosion in different tillage systems on stagnosol Croatia (1997)

U radu se govori o problematici erozije pri razlicitim nacinima obrade i uzgoju razlicitih kultura. Ukazuje se na jaku eroziju pri uzgoju jarina rijetkog sklopa, osobito na obradi uz/niz nagib, dok je pri uzgoju strnina gustog sklopa bez obzira na nacin obrade erozija izrazito mala.
Mechanisms of Linezolid Resistance among Coagulase-Negative Staphylococci Determined by Whole-Genome Sequencing (2014)

Linezolid is one of a limited number of antimicrobials available to treat drug-resistant Gram-positive bacteria, but resistance has begun to emerge. We evaluated the genomes of 28 linezolid-resistant staphylococci isolated from patients. Multiple mutations in the rRNA and associated proteins previously associated with linezolid resistance were found in the isolates investigated, underscoring the multifocal nature of resistance to linezolid in Staphylococcus. Importantly, almost half the S. epidermidis isolates studied harbored a plasmid-borne cfr RNA methylase gene, suggesting that the incidence of cfr may be higher in the United States than previously documented. This finding has important implications for infection control practices in the United States. Further, cfr is commonly detected in bacteria isolated from livestock, where the use of phenicols, lincosamides, and pleuromutilins in veterinary medicine may provide selective pressure and lead to maintenance of this gene in animal bacteria.
Effects of local injection of corticosteroids on the healing of ligaments. A follow-up report. (1995)

One hundred and one skeletally mature New Zealand White rabbits were used to study the long-term effects of a single injection of corticosteroid on the biomechanical, histological, and biochemical properties of ligament-healing. Two steroid doses were studied, as previously described. The injections were made into a fascial pocket immediately after transection of the ligament. The animals were killed forty-two and eighty-four days after the injury. In our previous investigation, in which we examined the early (inflammatory and proliferative) phases of ligament-healing, the specimens that had been injected with a dose of steroids equivalent to that given to humans demonstrated significantly inferior biomechanical properties and histological organization relative to controls that had not received an injection. In the current study, we examined the later (remodeling and maturation) phases of ligament-healing and found that the tensile strength (the ultimate stress) of the specimens that had been injected with the steroids returned to a value that was equal to that of the controls that had not received an injection; however, the peak load of the specimens that had been injected with steroids remained inferior to that of the controls. This was accompanied by a lag in the histological maturation.
Integration of microcoils for on-chip immunosensors based on magnetic nanoparticles capture (2016)

Immunoassays using magnetic nanoparticles (MNP) are generally performed under the control of permanent magnet close to the micro-tube of reaction. Using a magnet gives a powerful method for driving MNP but remains unreliable or insufficient for a fully integrated immunoassay on lab-on-chip. The aim of this study is to develop a novel lab-on-chip concept for high efficient immunoassays to detect ovalbumin (Biodefense model molecule) with microcoils employed for trapping MNP during the biofunctionalization steps. The objectives are essentially to optimize their efficiency for biological recognition by assuring a better bioactivity (antibodies-ovalbumin), and detect small concentrations of the targeted protein (~ 10 pg/mL). In this work, we studied the response of immunoassays complex function of ovalbumin concentration. The impact of MNP diameter in the biografting protocol was studied and permitted to choose a convenient MNP size for efficient biorecognition. We realized different immunoassays by controlling MNP in test tube and in microfluidic device using a permanent magnet. The comparison between these two experiments allows us to highlight an improvement of the limit of detection in microfluidic conditions by controlling MNP trapping with a magnet.
Redox control of secondary structure in a designed peptide (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTRedox control of secondary structure in a designed peptideGregory P. Dado and Samuel H. GellmanCite this: J. Am. Chem. Soc. 1993, 115, 26, 12609–12610Publication Date (Print):December 1, 1993Publication History Published online1 May 2002Published inissue 1 December 1993https://pubs.acs.org/doi/10.1021/ja00079a060https://doi.org/10.1021/ja00079a060research-articleACS PublicationsRequest reuse permissionsArticle Views494Altmetric-Citations106LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Solution structures of large Group 5A and 6A polyoxoanions by oxygen-17 nuclear magnetic resonance (1975)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSolution structures of large Group 5A and 6A polyoxoanions by oxygen-17 nuclear magnetic resonanceA. D. English, J. P. Jesson, W. G. Klemperer, T. Mamouneas, L. Messerle, W. Shum, and A. TramontanoCite this: J. Am. Chem. Soc. 1975, 97, 16, 4785–4786Publication Date (Print):August 1, 1975Publication History Published online1 May 2002Published inissue 1 August 1975https://pubs.acs.org/doi/10.1021/ja00849a067https://doi.org/10.1021/ja00849a067research-articleACS PublicationsRequest reuse permissionsArticle Views110Altmetric-Citations27LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
GITR signaling potentiates airway hyperresponsiveness by enhancing Th2 cell activity in a mouse model of asthma (2009)

GITR exerts a differential effect on cytokine release of fully differentiated Th1 and Th2 cells in vitro, potentiating Th2 but not Th1 cytokine production. This effect on Th2 effector functions was also observed in vivo in our mouse model of asthma, resulting in enhanced AHR, serum IgE responses and Th2 cytokine production. This is the first report showing the effects of GITR activation on cytokine production by polarized primary Th1 and Th2 populations and the relevance of this pathway for AHR in mouse models for asthma. Our data provides crucial information on the mode of action of the GITR signaling, a pathway which is currently being considered for therapeutic intervention.
Bethanidine increases one type of potassium current and relaxes aortic muscle (1988)

Using a whole-cell voltage-clamp technique, we identified two time- and voltage-dependent K+ currents: an early outward rectifier and a delayed outward rectifier in single vascular smooth muscle cells of rabbit aorta in culture. About 90% of the single cells tested showed a predominant delayed outward K+ current type. Both K+ currents were decreased by tetraethylammonium. In contrast, bethanidine sulphate (10(-4)M), a pharmacological analog of the cardiac antifibrillatory drug, bretylium tosylate, decreased the early outward K+ current, increased the delayed rectifier K+ current type, and hyperpolarized the resting membrane potential. Bethanidine was found to relax vascular smooth muscle. The vasodilatory effect of bethanidine is associated with the activation of a K+ current that is probably involved in keeping the membrane potential at the resting state, thereby depressing the excitability of the aortic vascular smooth muscle.
Inactivation of the HIF-1α/PDK3 Signaling Axis Drives Melanoma toward Mitochondrial Oxidative Metabolism and Potentiates the Therapeutic Activity of Pro-Oxidants (2012)

Cancer cells can undergo a metabolic reprogramming from oxidative phosphorylation to glycolysis that allows them to adapt to nutrient-poor microenvironments, thereby imposing a selection for aggressive variants. However, the mechanisms underlying this reprogramming are not fully understood. Using complementary approaches in validated cell lines and freshly obtained human specimens, we report here that mitochondrial respiration and oxidative phosphorylation are slowed in metastatic melanomas, even under normoxic conditions due to the persistence of a high nuclear expression of hypoxia-inducible factor-1α (HIF-1α). Pharmacologic or genetic blockades of the HIF-1α pathway decreased glycolysis and promoted mitochondrial respiration via specific reduction in the expression of pyruvate dehydrogenase kinase-3 (PDK3). Inhibiting PDK3 activity by dichloroacetate (DCA) or siRNA-mediated attenuation was sufficient to increase pyruvate dehydrogenase activity, oxidative phosphorylation, and mitochondrial reactive oxygen species generation. Notably, DCA potentiated the antitumor effects of elesclomol, a pro-oxidative drug currently in clinical development, both by limiting cell proliferation and promoting cell death. Interestingly, this combination was also effective against BRAF V600E-mutant melanoma cells that were resistant to the BRAF inhibitor vemurafenib. Cotreatment of melanomas with DCA and elesclomol in vivo achieved a more durable response than single agent alone. Our findings offer a preclinical validation of the HIF-1/PDK3 bioenergetic pathway as a new target for therapeutic intervention in metastatic melanoma, opening the door to innovative combinations that might eradicate this disease.
Spin effects in p<sup>+</sup>-i-n<sup>+</sup>a-Si:H cells; photovoltaic detected magnetic resonance (PDMR) (1983)

This paper reports spin-dependent photovoltaic effects in p+-i-n+ a-Si:H cells. The preliminary results are important for two reasons. Firstly, the method of photovoltaic detected magnetic resonance (PDMR) complements the well known optically detected magnetic resonance (ODMR) technique as a means for investigating recombination processes in these devices, particularly since it is sensitive to all radiative and non-radiative processes. Secondly, the remarkable sensitivity of PDMR even up to room temperature suggests that the method is suitable for routine assessment of both amorphous and crystalline photovoltaic junctions and barrier devices.
Investigation of Vascular Changes Following Penile Vein Ligation (1994)

In an effort to characterize the changes in penile vasculature that occur following penile vein ligation, we performed pharmaco-cavernosometry and pharmaco-cavernosography on 20 patients after penile vein ligation for comparison with preoperative studies. Three patients with return of erectile function underwent repeat study: 2 were completely normal and 1 had a mild leak from the deep dorsal vein. The remaining 17 patients had continued complaints of erectile impairment. Of these studies 4 showed no evidence of venous leakage, 11 identified a new site of leakage (7 corporo-spongiosal shunts, 2 crural veins and 2 with multiple sites of involvement) and 1 revealed persistent leak through the proximal stump of the resected deep dorsal vein, while 1 patient had an iodine allergy and underwent pharmaco-cavernosometry only. A repeat study in the latter patient showed flow volumes consistent with continued venous leakage. In summary, penile vein ligation appears to be effective at correcting venous leakage noted on cavernosography. However, new sites of leakage frequently appear postoperatively. A corporo-spongiosal shunt was the most frequent site of recurrent venous leakage. A surprisingly high percentage of patients with continued complaints of erectile dysfunction following penile vein ligation demonstrate no venous leakage on subsequent pharmaco-cavernosography.
Numerical Simulation of a Scroll Expander for Use in a Rankine Cycle (2008)

peer reviewed
Optimizing Hardware Design by Composing Utility-Directed Transformations (2011)

Utility-directed transformations involve changing a design to optimize for given constraints while preserving behavior. These changes are often achieved by techniques such as linear programming or geometric programming. We present a systematic approach composing multiple utility-directed transformations for optimizing and mapping a sequential design onto a customizable parallel computing platform such as a Field-Programmable Gate Array (FPGA). Our aim is to enable automatic design optimization at compile time. Design goals specified by users drive the design transformations. Each utility-directed transformation achieves part of the overall goal, and multiple utility-directed transformations, connected by pattern-directed transformations, are composed to fulfill the overall design requirements. The utility-directed transformations in this work produce performance-optimized designs by exploiting data reuse, MapReduce, and pipelining for the target parallel computing platform. Moreover, it is shown that performing transformations in different orders allows users to trade speed for resources, and design performance for compile time. Several applications are used to evaluate this approach on FPGAs. The system performance of a 64-bit matrix multiplication is shown to improve up to 98 times compared to the original design, in the target hardware platform.
Dipole Moments of Metal Chelate Compounds. IV. Metal Chelates of Analogs and Polar Substituted Analogs of Bisacetylacetoneethylenediimine<sup>1,2</sup> (1960)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDipole Moments of Metal Chelate Compounds. IV. Metal Chelates of Analogs and Polar Substituted Analogs of Bisacetylacetoneethylenediimine1,2Richard J. Hovey and Arthur E. MartellCite this: J. Am. Chem. Soc. 1960, 82, 11, 2697–2700Publication Date (Print):June 1, 1960Publication History Published online1 May 2002Published inissue 1 June 1960https://pubs.acs.org/doi/10.1021/ja01496a011https://doi.org/10.1021/ja01496a011research-articleACS PublicationsRequest reuse permissionsArticle Views71Altmetric-Citations6LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Heterocycles from keto acids with amino alcohols, diamines, and mercaptoamines (1969)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTHeterocycles from keto acids with amino alcohols, diamines, and mercaptoaminesPaul Aeberli and William J. HoulihanCite this: J. Org. Chem. 1969, 34, 1, 165–170Publication Date (Print):January 1, 1969Publication History Published online1 May 2002Published inissue 1 January 1969https://pubs.acs.org/doi/10.1021/jo00838a036https://doi.org/10.1021/jo00838a036research-articleACS PublicationsRequest reuse permissionsArticle Views256Altmetric-Citations39LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Correlation between plasma estradiol and estrone-3-glucuronide in urine during the monitoring of ovarian induction therapy. (1989)

An improved radioimmunoassay was developed to determine estrone-3-glucuronide in daily urine. Resulting levels were compared with those of estradiol in plasma of 10 healthy women and 14 undergoing ovulation induction therapy with human menopausal gonadotropin and human chorionic gonadotropin. A highly significant correlation between plasma estradiol and urinary estrone-3-glucuronide in normal (r = .9209; P less than .01) and stimulated (r = .9229; P less than .01) women was demonstrated. These results proved that the pattern of excretion of estrone-3-glucuronide perfectly reflected the changes in plasmatic estradiol levels when monitoring ovarian induction and that estrone-3-glucuronide determinations can provide clinically useful information in human induction therapy.
Antiarrhythmic drugs (1985)

PreviewVentricular arrhythmias can be life-threatening or benign, and they often are difficult to treat. Dr Roden examines the benefits and the limitations of the antiarrhythmics currently available and the agents that are likely to be on the market shortly.