gebhart/patrepeval huggingface dataset:
Calcium regulated chloride permeabilities in primary cultures of rabbit colonocytes (1996)

To determine if calcium-dependent secretagogues directly act on epithelial cells to elicit Cl- secretion, their effects on Cl- transport and intracellular Ca(2+) concentrations ([Ca2+]i) were determined in primary cultures of rabbit distal colonic crypt cells. The Cl- sensitive fluorescent probe, 6-methoxyquinolyl acetoethyl ester, MQAE and the Ca(2+)-sensitive fluorescent probe, fura-2AM were used to assess Cl- transport and [Ca2+]i, respectively. Basal Cl- transport (0.274 +/- 0.09 mM/sec) was inhibited significantly by the Cl- channel blocker diphenylamine-2-carboxylate (DPC, 50 microM, 0.068 +/- 0.02 mM/sec; P < 0.001) and the Na+/K+/ 2Cl- cotransport inhibitor furosemide (1 microM, 0.137 +/- 0.04 mM/sec; P < 0.01). Ion substitution studies using different halides revealed the basal influx to be l- > F- > or = Cl- > Br-. DPC inhibited l- influx by approximately 50%, F- influx by 80%, Cl-influx by 85%, and Br- influx by 90%. Furosemide significantly inhibited influx of Br- (84%) and Cl- (81%) but not of F- and l-. The effects of agents known to alter biological response by increasing [Ca2+]i in other epithelial systems were used to stimulate Cl- transport. Cl- influx in mM/second was stimulated by 1 microM histamine (0.58 +/- 0.05), 10 microM neurotensin (2.07 +/- 0.32), 1 microM serotonin (1.63 +/- 0.28), and 0.1 microM of the Ca2+ ionophore A23187 (2.05 +/- 0.40). The Cl- permeability stimulated by neurotensin, serotonin, and A23187 was partially blocked by DPC or furosemide added alone or in combination. Histamine-induced Cl- influx was significantly inhibited by only furosemide. Indomethacin blocked histamine-stimulated Cl- permeability but had no effect on the actions of the other agents. These studies, focusing on isolated colonocytes without the contribution of submucosal elements, reveal that (1) histamine stimulates Cl- transport by activating the Na+/K+/2Cl- cotransporter via a cyclooxygenase-dependent pathway; (2) neurotensin, serotonin, and A23187 activate both Cl- channels and the cotransporter, and their actions are cyclooxygenase-independent.
Chimeric Hormones and Neuropeptides (1997)

Correspondence| December 01 1997 Chimeric Hormones and Neuropeptides John Howl; John Howl 1School of Biochemistry, University of Birmingham, Edgbaston, Birmingham BI5 211, U.K. Search for other works by this author on: This Site PubMed Google Scholar ülo Langel; ülo Langel *Stockholm University, The Arrhenius Laboratories for Natural Sciences, Department of Neurochemistry and Neurotoxicology, S-1-0691 Stockholm, Sweden Search for other works by this author on: This Site PubMed Google Scholar Mark Wheatley Mark Wheatley 1School of Biochemistry, University of Birmingham, Edgbaston, Birmingham BI5 211, U.K. Search for other works by this author on: This Site PubMed Google Scholar Clin Sci (Lond) (1997) 93 (6): 605–606. https://doi.org/10.1042/cs0930605 Article history Received: August 13 1997 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn MailTo Cite Icon Cite Get Permissions Citation John Howl, ülo Langel, Mark Wheatley; Chimeric Hormones and Neuropeptides. Clin Sci (Lond) 1 December 1997; 93 (6): 605–606. doi: https://doi.org/10.1042/cs0930605 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsClinical Science Search Advanced Search This content is only available as a PDF. © 1997 The Biochemical Society and the Medical Research Society1997 Article PDF first page preview Close Modal You do not currently have access to this content.
New Methods for the Analysis of Heterogeneous Polysaccharides – <i>Lessons Learned from the Heparin Crisis</i> (2017)

Analysis of heterogeneous polymers, varying in size and substitution pattern, presents a challenge that cannot be met by direct comparisons with a single standard sample. A series of related approaches have been developed to meet this challenge, based on considering the polymers as a population, and applying methods to ascertain degrees of similarity employing first, multivariate analysis, and then two-dimensional correlation spectroscopy formed from their 1-dimensional 1H NMR spectra. Developments of this approach allow the sensitive detection of structural variation in test samples, the detection of alien materials, as well as identification of both native and aberrant structures with unprecedented sensitivity. The techniques are suitable for any situation in which the quality control of heterogeneous products needs to be analysed, including biosimilar and generic materials as well as other pharmaceutical products.
Analysis of multi‐user detection of multi‐rate transmissions in multi‐cellular CDMA (2008)

Abstract In this paper, we address the issue of multi‐user receiver design in realistic multi‐cellular and multi‐rate CDMA systems based on performance analysis. We consider the multi‐user detection (MUD) technique, denoted interference subspace rejection (ISR), because it offers a wide range of canonic suppression modes that range in performance and complexity between interference cancellers and linear receivers. To further broaden our study, we propose a modified ISR scheme called hybrid ISR to cope better with multi‐rate transmissions. The performance analysis, which is based on the Gaussian assumption (GA) and validated by simulations, takes into account data estimation errors, carrier frequency mismatch, imperfect power control, identification errors of time‐varying multipath Rayleigh channels and intercell interference. This analysis enables us to optimize the selection of the MUD mode for multi‐rate transmissions in different operating conditions. The effectiveness of interference cancellation is indeed investigated under different mobile speeds, numbers of receiving antennas, near‐far situations, channel estimation errors, and out‐cell to in‐cell interference ratios. This investigation suggests that the out‐of‐cell interference, the residual in‐cell interference, the noise enhancement as well as low mobility favor the simplest MUD modes as they offer the best performance/complexity tradeoffs. Copyright © 2008 John Wiley & Sons, Ltd.
Calamitic Liquid Crystalline Molecules with Lateral Intermolecular Hydrogen Bonding (2005)

ABSTRACT Calamitic liquid crystalline amides 1–3 were synthesized and their thermodynamic behaviors were investigated. Bent-rod shaped compound 1 exhibited a usual smectic C phase. On the other hand, in the case of straight-rod compound 2, two kinds of smectic C phases (SmCx and SmCy) were observed above the crystal phase. Although SmCy phase belonged to general smectic C phases, SmCx phase exhibited very sharp lines in its schlieren textures. As the packing model of smectic Cx phase, a lateral linear arrangement of the molecules was proposed to explain the difference between these smectic C phases. Additionally, in the case of 3 which has a flexible spacer between its amide group and straight rodlike core, the molecules generated the highly ordered smectic C (3a, SmCx) and a chiral cubic phase (3b). Keywords: amidechiral cubiclateral intermolecular hydrogen bondingliquid crystalsupra-structure
Sulfur addition to aldimines: thioamides, not thiaziridines, as products; revision of an old report (2005)

The reaction of 4-(phenylimino)butan-2-ol with ammonium polysulfide in refluxing EtOH yields 3-hydroxy-N-phenylbutanethioamide as the only isolated product. The structure has been established by single-crystal X-ray diffraction. Treatment of N-benzylidenamines with ammonium polysulfide has proven a general method for the preparation of thiobenzamides.
Prognostic impact of primary tumor site location in metastatic colorectal cancer (mCRC). (2016)

578 Background: CRC site is associated with differences in key molecular features that may impact survival in the metastatic (met) setting. We assessed the magnitude of outcome differences in a non-clinical trial scenario and explored how rectum cancer (RC) compares with right- and left-sided (RS and LS) colon tumors. Methods: Retrospective analysis of clinicopathological and molecular data from all mCRC patients (pts) treated at VHIO from January 2010 to June 2015. Targeted mutation (mut) profiling of driver oncogenes was performed by mass spectrometry (MassARRAY, Sequenom) or with next generation sequencing (Amplicon MiSeq, Illumina). Survival after relapse (SAR) was defined as time from diagnosis of met disease to death. Tumor site was categorized as RS (cecum, ascending, hepatic flexure, and transverse), LS (splenic flexure, descending, and sigmoid) or RC. We performed Cox proportional hazards models and adjusted for covariates including number and location of met sites, surgical resection of met lesions, and KRAS, BRAF and PIK3CA mut. Results: From 686 pts analyzed, primary site was RS/LS/RC in 29.4%/43.4%/27.2%, respectively. Pts with LS tumors were less likely to have ≥ 2 met sites (32%/20%/29%; p = 0.01). Lung metastases were more common in RC pts (20%/15%/36%; p < 0.001), peritoneal metastasis in pts with RS tumors (28%/14%/5%, p < 0.001). Surgical resection of any met disease was performed less frequently in pts with RS tumors (28%/46%/41%; p < 0.001). KRAS mut (59%/45%/44%), BRAF V600E mut (9%/5%/3%) and PIK3CAmut (25%/14%/11%) were more prevalent in RS tumors. In the overall population with survival data (n = 606, 424 events), SAR was significantly lower in pts with RS tumors (31/46/46 months; RS vs. LS HR = 1.54 [1.2-2.0], p < 0.001; rectum vs. left HR = 1.0 [0.7-1.3], p = 0.83). For comparison, worse SAR was also seen in the subset of pts (n = 337; 276 events) not eligible for surgical resection of met disease (26/31/35 months; right vs. left HR = 1.60 [1.2-2.2], p = 0.004; rectum vs. left HR = 0.9 [0.6-1.2], p = 0.42). Conclusions: The worse SAR of pts diagnosed with right-sided CRC tumors cannot be explained only by their distinctive met pattern or mutation profile of common oncogenes. Left-sided and rectum tumors have similar outcomes.
Coronary artery lumen volume measurement using three‐dimensional intravascular ultrasound: Validation of a new technique (1994)

Three-dimensional intravascular ultrasound is a new technique that can accurately measure coronary artery lumen volumes. Further technical improvements may help to establish this technique as the new standard for lumen volume measurement.
Über den Chemismus der Carbonylgruppen‐Reduktion aromatischer Aldehyde an der Quecksilbertropfelektrode (1953)

Abstract Auf Grund polarographischer Untersuchungen des Reduktionsverhaltens von Benzaldehyd in einem weiten p H ‐Bereich (1 bis 13) wird ein Reaktionsschema der Kathodenvorgänge gegeben. In sauren und alkalischen Lösungen, sowie im Übergangsbereich treten verschiedene Reduktionsprodukte auf. Dies wird durch die unterschiedliche Reaktionsfolge auf das sich primär — p H ‐abhängig — einstellende Dissoziationsgleichgewicht des Radikals C 6 H 5 CHOH erklärt. Die Gleichgewichtskonstante der Dissoziationsreaktion wird aus der Verringerung der Diffusionsstromstärke mit dem p H im alkalischen Gebiet zwischen K = 10 −9 und 10 −10 eingegrenzt.
Analysis of Transient Flow in Supersonic Micronozzles (2011)

Covers advancements in spacecraft and tactical and strategic missile systems, including subsystem design and application, mission design and analysis, materials and structures, developments in space sciences, space processing and manufacturing, space operations, and applications of space technologies to other fields.
Complement-mediated thrombotic microangiopathy as a link between endothelial damage and steroid-refractory GVHD (2018)

Transplant-associated thrombotic microangiopathy (TA-TMA), a complication of hematopoietic cell transplant (HCT), is associated with significant morbidity and mortality. The pathophysiology and overlap of TA-TMA with other posttransplant complications such as graft-versus-host disease (GVHD) is poorly understood. We retrospectively identified cases of TA-TMA among patients with grade 3/4 gastrointestinal (GI) GVHD, reviewed intestinal biopsy specimens, and performed correlative testing of biomarkers associated with TA-TMA. TA-TMA was more common in patients with steroid-refractory GVHD compared with steroid-responsive GVHD (79.3% vs 42.1%; <i>P</i> = .001). Among patients surviving 100 days post-HCT, 1-year survival from day 100 was significantly better for patients who had not developed TA-TMA in the first 100 days (69.5% vs 36.7%; <i>P</i> < .001). Only 1 of 7 proposed TA-TMA histology criteria (mucosal hemorrhage) differed significantly based on GVHD steroid response. In multivariable modeling, steroid-refractory GVHD was a risk factor for development of TA-TMA (hazard ratio, 3.09; 95% confidence interval, 1.68-5.67; <i>P</i> < .001). There were no differences in complement activation at GVHD onset; however, 2 to 6 weeks later, patients with TA-TMA had higher levels of BBPlus and C5b-9, markers of alternative and terminal pathway activation (BBPlus: median, 600 vs 209.3 ng/mL; <i>P</i> = .0045) (C5b-9: median, 425.9 vs 258.4 ng/mL; <i>P</i> = .029). TA-TMA is associated with poor overall survival (OS) following HCT and may be detected early by histologic findings and may be differentiated from GVHD by measurement of alternative and terminal complement pathway activation. It is unknown whether treatment of TA-TMA will improve survival in steroid-refractory GVHD.
Prevalence of intestinal parasites in food handlers in a rural community in Cojedes, Venezuela (2012)

Aim: Intestinal parasites are a public health problem due to their high prevalence. Handlers of food contaminated with parasites become potential sources of infection because some parasitic forms are transmitted directly from the source of infection to the host through the fecal-oral route. In addition, there is an increased consumption of food outside the household by residents of urban and rural areas. The objective is to collect information so that health authorities implement health programs appropriate to each region. Methods: Descriptive and cross-sectional field-study. Sixty-four food handlers participated but the sample was composed of 50. A questionnaire on hygienic practices in food handling was administered. The socioeconomic status was determined, as well as prevalence of parasites by performing stool tests.
Radiomic Phenotypes Distinguish Atypical Teratoid/Rhabdoid Tumors from Medulloblastoma (2021)

Six quantitative signatures of image intensity, texture, and morphology distinguish atypical teratoid/rhabdoid tumors from medulloblastomas with high prediction performance across different machine learning strategies. Use of this technique for preoperative diagnosis of atypical teratoid/rhabdoid tumors could significantly inform therapeutic strategies and patient care discussions.
Optically Active Cyclopentanediol Synthetic Building Blocks from Tartaric Acid (1986)

Two enantiomeric five-membered ring building blocks from the same educt: This was achieved by reaction of 1 with two different nucleophilic reagents. 1,4-Addition of R2CuLi to 1 and cleavage of MeOH furnished (R,R)-2; 1,2-Addition of RM to 1, allyl rearrangement, and MeOH-cleavage lead to the mirror image (S,S)-2.
<i>RESEARCH</i> : Fluorescence Microscopy–Based Protocol for Detecting Residual Bacteria on Medical Devices (2020)

Standard methods are needed to reliably and efficiently assess bacterial contamination of processed medical devices. This article demonstrates a standard operating procedure (SOP) for fluorescence microscopy-based detection of residual bacteria on medical devices (BAC-VIS). BAC-VIS uses a 4',6-diamidino-2-phenylindole (DAPI) stain with fluorescent microscopy to quickly and cost-effectively detect bacterial contamination of processed medical device parts. The BAC-VIS protocol was optimized and achieved greater than 80% staining efficiency and a signal-to-noise ratio of more than 20 using four representative organisms. The SOP was first validated for use on a buildup biofilm model, accessory channels of contaminated clinically used devices, and inoculated endoscope end caps and O-rings. The buildup biofilm model was used to evaluate BAC-VIS after repeated treatment of adherent bacteria with three common high-level disinfectants: glutaraldehyde, ortho-phthalaldehyde, and peracetic acid. Next, BAC-VIS was used to assess clinically used endoscope parts that cultured positive for Gram-negative bacteria. DAPI-stained cells were found on all culture-positive devices, especially in grooves and imperfections on the surface. Finally, BAC-VIS was used to detect bacteria on inoculated endoscope device components. The results showed potential for BAC-VIS to be a valuable tool for industry and academic/medical researchers for investigations of contaminated medical devices. Results obtained using BAC-VIS can increase understanding of the role of design in cleanability, wear, and prevention of contamination and may lead to improvements in materials and design that could make processed endoscope use safer for patients. Of note, this protocol is not for detecting bacteria on scopes or scope parts that will be put back into clinical use.
Quantum dots-in-a-well infrared photodetectors for long wavelength infrared detection (2006)

We report on a quantum dots-in-a-well infrared photodetector (DWELL QDIP) grown by metal organic vapor phase epitaxy. The DWELL QDIP consisted of ten stacked InAs/In<sub>0.15</sub>Ga<sub>0.85</sub>As/GaAs QD layers embedded between n-doped contact layers. The density of the QDs was about 9 x 10<sup>10</sup> cm<sup>-2</sup> per QD layer. The energy level structure of the DWELL was revealed by optical measurements of interband transitions, and from a comparison with this energy level scheme the origin of the photocurrent peaks could be identified. The main intersubband transition contributing to the photocurrent was associated with the quantum dot ground state to the quantum well excited state transition. The performance of the DWELL QDIPs was evaluated regarding responsivity and dark current for temperatures between 15 K and 77 K. The photocurrent spectrum was dominated by a LWIR peak, with a peak wavelength at 8.4 μm and a full width at half maximum (FWHM) of 1.1 μm. At an operating temperature of 65 K, the peak responsivity was 30 mA/W at an applied bias of 4 V and the dark current was 1.2×10<sup>-5</sup> A/cm<sup>2</sup>. Wavelength tuning from 8.4 μm to 9.5 μm was demonstrated, by reversing the bias of the detector.
Impact of Oil-derived Ash on Continuous Regeneration-type Diesel Particulate Filter - JCAPII Oil WG Report (2004)

<div class="htmlview paragraph">Impact of oil-derived ash on the pressure drop of continuous regeneration-type diesel particulate filter (CR-DPF) was investigated through 600hrs running test at maximum power point on a 6.9L diesel engine, which meets the Japanese long-term emission regulations enacted in 1998, using approximately 50ppm sulfur content fuel. Sulfated ash content of test oils were varied as 0.96, 1.31, and 1.70 mass%, respectively. During the running test, the exhaust pressure drop through CR-DPF was measured. And after the test, the ventilation resistance through CR-DPF was also evaluated before and after the baking process, which was applied to eliminate the effect of soot accumulated in CR-DPF.</div> <div class="htmlview paragraph">The results revealed that the less sulfated ash in oil gave rise to lower pressure drop across CR-DPF. According to microscope examination of the baked DPF, ash was mainly accumulated on the wall surface of CR-DPF, and that seemed to be related to the magnitude of pressure drop caused by ash. The main component of ash on CR-DPF was CaSO<sub>4</sub>, of which Ca is derived from oil.</div> <div class="htmlview paragraph">Metal content in exhaust gas was also analyzed at the engine out. It was found that Ca content at the engine out had increased proportionally with Ca content in oil. Furthermore, when compared at engine out and behind CR-DPF, it was found that over 95% of oil-derived metal in exhaust gas could be trapped by CR-DPF, irrespective of exhaust gas condition (flow rate and temperature) and oil additive formulation.</div> <div class="htmlview paragraph">The oil with less sulfated ash can reduce the accumulation of oil-derived ash on DPF, and thus the pressure drop through DPF. Therefore, it will contribute to prolong the maintenance interval of the DPF.</div>
The design of methane activation catalysts (1988)

The goal of Sandia's methane activation program is to use computer-aided-design (CAD) techniques to guide the synthesis of catalysts for the direct conversion of methane. Several enzymes, such as cytochrome P/sub 450/ and methane monooxygenase, catalyze the oxidation of alkanes to alcohols at low temperatures and pressures. we are attempting to design catalysts that mimic these enzymes. Our CAD studies on porphyrins, which are the active sites in many enzymes, are being combined with 1) structural characterization of naturally occuring catalysts; 2) the development of activity and selectivity tests; and 3) the synthesis and characterization of designed catalysts. Results of CAD studies on enzymes that carry out alkane activation or perform C/sub 1/ chemistry and on unique synthetic porphyrins have shown that the synthetic porphyrins can have site configurations analogous to these enzymes. The CAD work also uses molecular-energy minimization and dynamics calculations to evaluate methane interactions with the synthetic porphyrins. Activity tests have been set up for cyclohexane, hexane and butane with additional tests being developed for methane and ethane. Synthesis of designed porphyrins is currently underway. 9 refs., 4 figs.
Sleep Complaints as Early Predictors of Posttraumatic Stress Disorder: A 1-Year Prospective Study of Injured Survivors of Motor Vehicle Accidents (2002)

These results suggest that on the basis of sleep complaints as early as 1 month after the trauma, it is possible to detect subjects who will later develop chronic PTSD.
Picosecond study of an intermediate in the <i>trans</i> to <i>cis</i> isomerization pathway of stiff stilbene (1984)

A picosecond spectroscopic study of trans 1,1′-biindanylidene (‘‘stiff’’ stilbene) has exposed a transient absorption at 351 nm which is not an electronically excited state of the trans or cis forms. The signal strength increases with decreasing viscosity in a manner that is consistent with the decay of the excited trans molecules being the precursor of the new transient. The data suggest that after the viscosity dependent formation of the intermediate, it decays with a time constant of 10±3 ps into cis stiff stilbene. It is suggested that the intermediate is the ‘‘phantom’’ state of stilbene photochemistry.
Back-focal-plane interferometry: position or force detection? (2011)

We recently showed that force measurements through the detection of beam momentum changes can be implemented in single-beam gradient traps. We thereby achieved a method that renders force measurements insensitive to sample's geometrical and optical properties. Our instrument could be calibrated by a parameter that remained constant within a 4% error when, under comparable conditions, the traditional approach based on position detection could change by a factor of two or more. The setup required for these measurements was but a modified version of the apparatus used for position detection with back-focal-plane interferometry (BFPI). Despite the apparent difference between both techniques, we show here that they are not independent and we explicitly indicate the connection between them. The results suggest that our changes in the position detection instrument could have some important advantages and improve the technique when this is used to ultimately determine optical forces.