gebhart/patrepeval huggingface dataset:
Determination of Arsenic, Cadmium, Mercury, and Lead by Inductively Coupled Plasma/Mass Spectrometry in Foods after Pressure Digestion: NMKL Interlaboratory Study (2007)

Thirteen laboratories participated in an interlaboratory method performance (collaborative) study on a method for the determination of arsenic, cadmium, mercury, and lead by inductively coupled plasma/mass spectrometry (ICP/MS) after pressure digestion including the microwave heating technique. Prior to the study, the laboratories were able to practice on samples with defined element levels (pretrial test). The method was tested on a total of 7 foodstuffs: carrot puree, fish muscle, mushroom, graham flour, simulated diet, scampi, and mussel powder. The elemental concentrations in mg/kg dry matter (dm) ranged from 0.06-21.4 for As, 0.03-28.3 for Cd, 0.04-0.6 for Hg, and 0.01-2.4 for Pb. The materials used in the study were presented to the participants as blind duplicates, and the participants were asked to perform single determinations on each sample. The repeatability relative standard deviations (RSDr) for As ranged from 3.8 to 24%, for Cd from 2.6 to 6.9%, for Hg from 4.8 to 8.3%, and for Pb from 2.9 to 27%. The reproducibility relative standard deviations (RSDR) for As ranged from 9.0 to 28%, for Cd from 2.8 to 18%, for Hg from 9.9 to 24%, and for Pb from 8.0 to 50%. The HorRat values were less than 1.5 for all test samples, except for the determination of Pb in wheat flour at a level close to the limit of quantitation (0.01 mg/kg dm). The study showed that the ICP/MS method is satisfactory as a standard method for elemental determinations in foodstuffs.
Expression and intracellular transport of microvillus membrane hydrolases in human intestinal epithelial cells. (1985)

A panel of monoclonal antibodies was produced against purified microvillus membranes of human small intestinal enterocytes. By means of these probes three disaccharidases (sucrase-isomaltase, lactase-phlorizin hydrolase, and maltase-glucoamylase) and four peptidases (aminopeptidase N, dipeptidylpeptidase IV, angiotension I-converting enzyme, and p-aminobenzoic acid peptide hydrolase) were successfully identified as individual entities by SDS PAGE and localized in the microvillus border of the enterocytes by immunofluorescence microscopy. The antibodies were used to study the expression of small intestinal hydrolases in the colonic adenocarcinoma cell line Caco 2. This cell line was found to express sucrase-isomaltase, lactase-phlorizin hydrolase, aminopeptidase N, and dipeptidylpeptidase IV, but not the other three enzymes. Pulse-chase studies with [35S]methionine and analysis by subunit-specific monoclonal antibodies revealed that sucrase-isomaltase was synthesized and persisted as a single-chain protein comprising both subunits. Similarly, lactase-phlorizin hydrolase was synthesized as a large precursor about twice the size of the lactase subunits found in the human intestine. Aminopeptidase N and dipeptidylpeptidase IV, known to be dimeric enzymes in most mammals, were synthesized as monomers. Transport from the rough endoplasmic reticulum to the trans-Golgi apparatus was considerably faster for the peptidases than for the disaccharidases, as probed by endoglycosidase H sensitivity. These results suggest that the major disaccharidases share a common biosynthetic mechanism that differs from that for peptidases. Furthermore, the data indicate that the transport of microvillus membrane proteins to and through the Golgi apparatus is a selective process that may be mediated by transport receptors.
Global Environmental Governance and Regional Centers (2012)

As global environmental governance evolves, the parties to the 1989 Basel Convention on the Control of Transboundary Movements of Hazardous Wastes and Their Disposal and to the 2001 Stockholm Convention on Persistent Organic Pollutants have established regional centers working on capacity building and technology transfer. This article empirically explores the following questions: Why did the parties to the Basel and Stockholm Conventions establish these regional centers? What roles do the regional centers play in treaty implementation and multilevel governance? The article argues that the parties have set up regional centers in response to three partially overlapping sets of developing- and industrialized-country interests: expanding regional cooperation (both developing and industrialized countries); attracting more resources for treaty implementation (mainly developing countries); and supporting implementation projects across smaller groups of countries (mainly industrialized countries). This article finds that the regional centers collectively operate in three broad areas important to treaty implementation: raising awareness, strengthening administrative ability, and diffusing scientific and technical assistance and information. However, the ability of the regional centers to function effectively depends on access to greater resources and stronger political support. There may also be benefits to expanding regional center mandates into areas of monitoring and compliance to improve multilevel governance. Furthermore, the regional level should be given more consideration in the study of global environmental politics.
Evaluation of three commercially available blood culture systems for cultivation of Helicobacter pylori (1994)

Helicobacter pylori is unable to grow in regular blood culture systems, including the BACTEC (Johnston Laboratories), Septi-Chek (Hoffman-La Roche), and Bacto (Difco) systems. We tested three blood culture systems used for fastidious organisms: brucella broth with SPS and CO2 (Becton Dickinson), biphasic brain heart infusion agar or broth (Becton Dickinson), and supplemented peptone broth (Vacutainer). Blood culture bottles were inoculated with H. pylori and human blood and were then incubated by routine diagnostic laboratory procedures. All three blood culture systems were able to sustain the growth of H. pylori, but brucella broth had the highest CFU per milliliter after 72 h. We conclude that a diagnostic laboratory should be able to detect H. pylori bacteremia in a majority of cases by using brucella blood culture bottles.
Derivation of Effective Aerodynamic Surface Roughness in Urban Areas from Airborne Lidar Terrain Data (2008)

Abstract An automated technique was developed that uses only airborne lidar terrain data to derive the necessary parameters for calculation of effective aerodynamic surface roughness in urban areas. The technique provides parameters for geometric models that have been used over the past 40+ years by automatically deriving the relevant geometry, orientation, and spacing of buildings and trees. In its prototypical form, this technique subsequently calculates an effective surface roughness for 1-km2 parcels of land for each of five geometric models. The user can define several constraints to guide processing based on a priori knowledge of the urban area or lidar data characteristics. Any given wind direction (or range of directions) can be selected to simulate conditions of variable wind flow and the impact on effective surface roughness. The operation, capabilities, and limitations of the technique were demonstrated using lidar terrain data from Broward County, Florida.
Central Nervous System Toxicity of Cancer Therapy in Children (1995)

Cancer treatment of the central nervous system has contributed positively to long-term disease-free survival from childhood cancers such as acute leukemia and medulloblastoma. However, intrathecal chemotherapy and whole brain radiation are associated with acute, subacute, and delayed toxicities. The purpose of this review article is to summarize what is known about critical events in prenatal and postnatal brain development; the pathophysiology of radiation and chemotherapy neurotoxicity and clinical manifestations and risk factors for acute, subacute, and delayed toxicities. The importance of long-term follow-up and for multidisciplinary interventions that focus on prevention as well as management of identified problems are addressed.
Tadalafil in the management of severe pulmonary artery hypertension. (2015)

Severe oyknibart arterial hypertension is a life-threatening disease with a poor prognosis. Continuous intravenous infusion of prostacyclin has proved effective in this condition. However, it carries the risk of serious complications arising from the complex delivery system along with a high cost. Prostacyclin analogs, endothelin antagonists, and the phophodiesterase-5 inhibitor sildenafil are emerging and promisinge therapies. Tadalafil, like sildenafil, is a selective phosphodiesterase-5 inhibitor with a longer duration of action. We repport the use of tadalafil in two patients of severe pulmonary arterial hypertension who could not afford expensive treatment.
Supervisor Support in the Work Place: Legitimacy and Positive Affectivity (2000)

The authors tested 3 hypotheses regarding supervisor support in the work place. The validation hypothesis predicts that when employees are supported by their coworkers and the larger organization, they also receive more support from their supervisors. The positive affectivity hypothesis predicts that employees with positive dispositions receive more supervisor support because they are more socially oriented and likable. The moderation hypothesis predicts a joint multiplicative effect between validation and positive affectivity. An assessment of the hypotheses among a sample of 1,882 hospital employees in Korea provided strong support for the validation and moderation hypotheses.
Trona and Sodium Bicarbonate in Beef Cattle Diets: Effects on pH and Volatile Fatty Acid Concentrations (1987)

A study was conducted to evaluate the effectiveness of NaHCO3 and trona in beef cattle diets. Trace element (n = 28) analysis revealed no toxicological or safety concerns with the use of trona. Trona was more (P less than .05) soluble in ruminal fluid than Na2CO3, and NaHCO3 and had greater (P less than .05) buffering capacity (9.6 meq/g) than NaHCO3 (6.1 meq/g) but less (P less than .05) than Na2CO3 (11.1 meq/g). Calcium carbonate was insoluble and did not buffer ruminal fluid. Six yearling (avg 272 kg) Hereford X Angus steers, each with ruminal, duodenal and ileal cannulas, were fed 50:50 (cracked corn-based concentrate:cottonseed hulls) or 90:10 concentrate diets with no buffer, 1% NaHCO3 or with 1% trona. Intake, across all treatments, averaged 2.4% of body weight. Propionate (mmol/liter) increased (17.6 vs 13.5; P less than .05) and butyrate decreased (3.5 vs 5.2; P less than .05) with trona in the 90:10 diet as compared with no buffer. Propionate (16.8) increased (P less than .05) with NaHCO3 in the 90:10 diet. Average ruminal pH was greater (P less than .05) in 90:10 diets with trona or NaHCO3 than with no buffer (5.61, 5.61 vs 5.55); duodenal pH was greater (P less than .01) with trona than with no buffer (2.66 vs 2.55). Trona reduced ruminal pH-hours (P less than .05) and pH-area (P less than .12; time and area below mean pH of control) below control for both concentrate levels.(ABSTRACT TRUNCATED AT 250 WORDS)
Optimizing the management of acute ischaemic stroke: a review of the utilization of intravenous recombinant tissue plasminogen activator (tPA) (2012)

What is known and Objective: Thrombolysis using intravenous tissue plasminogen activator (tPA) is the only available evidence-based treatment for acute ischaemic stroke; however, its current utilization is very low. Therefore, the aim of this article is to review the literature regarding the use of intravenous tPA for the treatment of acute ischaemic stroke. The review will also compare utilization rates of thrombolysis in different centres across the world and identify key reasons for the underutilization of thrombolysis in stroke. Methods: MEDLINE, EMBASE, International Pharmaceutical Abstracts (IPA) and Google Scholar were searched for relevant original articles, review papers and other publications over the publication period 1995–2012. Results and Discussion: The National Institute of Neurological Disorders and Stroke (NINDS) (1995, N = 624 patients) and ECASS III (2008, N = 821 patients) are two pivotal randomized controlled trials providing evidence for the use of intravenous tPA within 3 h or 3–4·5 h from stroke onset, respectively. Both trials have shown that tPA administration decreases disability at 90 days from stroke. Furthermore, a recent pooled analysis of randomized controlled trials (2010, N = 3670 patients) supports these results, highlighting that early stroke treatment is associated with better outcomes, especially when treatment is started within 90 min of stroke onset (but suggesting that the benefit could be afforded within a 4·5-h time window). Three major observational trials, STARS (2000, N = 389 patients), CASES (2005, N = 1135 patients) and SITS-MOST (2007, N = 6483 patients), have reported acceptable safety and efficacy in clinical practice. However, only a small proportion of acute ischaemic stroke patients receive tPA in clinical practice, because of the limited availability of tPA-utilizing sites and suboptimal use of tPA in sites where it is available. What is new and Conclusion: tPA reduces disability in stroke patients. Moreover, acceptable safety has been demonstrated in routine clinical practice. However, tPA is significantly underutilized, and specific efforts are needed to encourage appropriate implementation of the stroke treatment guidelines to optimize the use of this important therapy.
The UL8 subunit of the herpes simplex virus helicase-primase complex is required for efficient primer utilization (1992)

The herpes simplex virus (HSV) type 1 helicase-primase is a three-protein complex, consisting of a 1:1:1 association of UL5, UL8, and UL52 gene products (J.J. Crute, T. Tsurumi, L. Zhu, S. K. Weller, P. D. Olivo, M. D. Challberg, E. S. Mocarski, and I. R. Lehman, Proc. Natl. Acad. Sci. USA 86:2186-2189, 1989). We have purified this complex, as well as a subcomplex consisting of UL5 and UL52 proteins, from insect cells infected with baculovirus recombinants expressing the appropriate gene products. In confirmation of previous reports, we find that whereas UL5 alone has greatly reduced DNA-dependent ATPase activity, the UL5/UL52 subcomplex retains the activities characteristic of the heterotrimer: DNA-dependent ATPase activity, DNA helicase activity, and the ability to prime DNA synthesis on a poly(dT) template. We also found that the primers made by the subcomplex are equal in length to those synthesized by the UL5/UL8/UL52 complex. In an effort to uncover a role for UL8 in HSV DNA replication, we have developed a model system for lagging-strand synthesis in which the primase activity of the helicase-primase complex is coupled to the activity of the HSV DNA polymerase on ICP8-coated single-stranded M13 DNA. Using this assay, we found that the UL8 subunit of the helicase-primase is critical for the efficient utilization of primers; in the absence of UL8, we detected essentially no elongation of primers despite the fact that the rate of primer synthesis on the same template is undiminished. Reconstitution of lagging-strand synthesis in the presence of UL5/UL52 was achieved by the addition of partially purified UL8. Essentially identical results were obtained when Escherichia coli DNA polymerase I was substituted for the HSV polymerase/UL42 complex. On the basis of these findings, we propose that UL8 acts to increase the efficiency of primer utilization by stabilizing the association between nascent oligoribonucleotide primers and template DNA.
A 255nW 138kHz RC oscillator for ultra-low power applications (2016)

An on-chip sub-μW RC oscillator suitable for ultra-low power applications is presented. A technique that cancels both comparator delay and offset are proposed to improve the frequency stability against variations of temperature and supply voltage. This relaxation oscillator is designed and simulated in a 180-nm CMOS technology. The post layout simulation results show that the proposed circuit has a 1-cycle start-up time, and the frequency drift is less than ±0.6%. For the supply voltage changing from 0.9 to 1.2 V, the variation of frequency is -0.72% ~ +0.68% and the temperature coefficient is 75.5 ppm/°C over 0 to 90°C. The power consumption of the proposed oscillator is 255 nW at TT and 27°C.
Vitamin D and manganese in the nutrition of the chick (1967)

1. In two experiments with chicks given diets of different manganese content, the effects of vitamin D on the Mn contents of bone and liver and on the retention of 54 Mn in bone, liver and the whole body were determined. 2. Vitamin D slightly increased the Mn content of dry fat-free bone but the proportion of Mn to ash remained unchanged and the Mn content of bone was influenced much more by the level of dietary Mn than by the presence of vitamin D. 3. Vitamin D, when given over a 3-week period, increased slightly the Mn content of the liver, but again the level of dietary Mn had a greater effect than the presence of vitamin D. 4. The retention of an oral dose of 54 Mn was not uniformly influenced by vitamin D, but in birds given the hign-Mn diet retention was reduced by vitamin D, indicating a decrease in the turnover of Mn.
Seismic data time-frequency domain filter with wavelet transform (2002)

In this paper, the Wavelet Transform is used to decompose every traces of the seismic record into different records. The purpose is to make seismic data filtering, to remove the noise and to improve both seismic signal-to-noise (S/N) ratio and resolution. Real result of the procession shows that the Wavelet Transform is a good method of removing noise and it is a new powerful digital filtering technique which is very practical and valuable.
Vision-based real-time game interface (2009)

In this paper, we will introduce a fast and robust method of constituting a probability map by extracting foreground object from background image which is basic technology to be applied to vision-based real-time game interaction. We implement a neural network with one hidden layer to recognize a motion of the probability map. In this process, we extract the foreground probability map which is robust against change of illumination using local binary pattern and improve the speed using integral histogram. Also, we use a novel Gaussian window frame mask which can be applied fast in the integral histogram process and reduce noise by adaptive Gaussian sigma value depending on the strength of edge. In the following, experiments are carried out and satisfactory results are obtained which indicate the fast and robust conditions.
A magnesium‐dependent <i>mreB</i> null mutant: implications for the role of <i>mreB</i> in <i>Bacillus subtilis</i> (2005)

MreB shares a common prokaryotic ancestor with actin and is present in almost all rod-shaped bacteria. MreB proteins have been implicated in a range of important cell processes, including cell morphogenesis, chromosome segregation and cell polarity. The mreB gene frequently lies at the beginning of a cluster of genes, immediately upstream of the conserved mreC and mreD genes. RNA analysis showed that in Bacillus subtilis mreB is co-transcribed with mreC and that these genes form part of an operon under the control of a promoter(s) upstream of mreB. Construction of an in-frame deletion of mreB and its complementation by mreB(+) only, in trans, established that the gene is important for maintenance of cell width and cell viability under normal growth conditions, independent of polar effects on downstream genes. Remarkably, virtually normal growth was restored to the mreB null mutant in the presence of high concentrations of magnesium, especially when high concentrations of the osmoprotectant, sucrose were also present. Under these conditions, cells could be maintained in the complete absence of an mreB gene, with almost normal morphology. No detectable effect on chromosome segregation was evident in the mutant, nor was there an effect on the topology of nascent peptidoglycan insertion. A GFP-MreB fusion was used to look at the localization of MreB in live cells. The pattern of localization was similar to that previously described, but no tight linkage to nucleoid positioning was evident. Propagation of the mreB null mutant in the absence of magnesium and sucrose led to a progressive increase in cell width, culminating in cell lysis. Cell division was also perturbed but this effect may be secondary to the disturbance in cell width. These results suggest that the major role of MreB in B. subtilis lies in the control of cell diameter.
A Programmable Digital Control System for Copying Machines (1974)

Machines whose functioning is dependant upon inputs and previous states can be controlled by a system that performs a series of compare and test operations only to determine the output states. For efficient operation such a system is directly linked to the input/output functions in the machine. At each step the controller decides to turn on/off, or do nothing to an output function. Its decision comes from machine inputs, operating mode and the correct time instant in the machine cycle. The comparison results decide the operation of the machine. Since the system has a memory it can be programmed for different functions and cause the machine which it controls to work differently. This programmability makes the controller similar to a computer. However, its main difference from a general computer is the few kinds of operations it is designed to perform. To be competitive with mini-computers in this application, the controller should be programmable; to compete with other implementations it must be cheaper to manufacture. A programmable digital controller for a Xerox Color Copying Machine is described. It is shown how the above concepts were reduced to hardware that was subsequently successfully demonstrated.
High-Throughput Analysis With 96-Capillary Array Electrophoresis and Integrated Sample Preparation for DNA Sequencing Based on Laser Induced Fluorescence Detection (2001)

The purpose of this research was to improve the fluorescence detection for the multiplexed capillary array electrophoresis, extend its use beyond the genomic analysis, and to develop an integrated micro-sample preparation system for high-throughput DNA sequencing. The authors first demonstrated multiplexed capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC) separations in a 96-capillary array system with laser-induced fluorescence detection. Migration times of four kinds of fluoresceins and six polyaromatic hydrocarbons (PAHs) are normalized to one of the capillaries using two internal standards. The relative standard deviations (RSD) after normalization are 0.6-1.4% for the fluoresceins and 0.1-1.5% for the PAHs. Quantitative calibration of the separations based on peak areas is also performed, again with substantial improvement over the raw data. This opens up the possibility of performing massively parallel separations for high-throughput chemical analysis for process monitoring, combinatorial synthesis, and clinical diagnosis. The authors further improved the fluorescence detection by step laser scanning. A computer-controlled galvanometer scanner is adapted for scanning a focused laser beam across a 96-capillary array for laser-induced fluorescence detection. The signal at a single photomultiplier tube is temporally sorted to distinguish among the capillaries. The limit of detection for fluorescein is 3 x 10-11 M (S/N = 3) for 5-mW of total laser power scanned at 4 Hz. The observed cross-talk among capillaries is 0.2%. Advantages include the efficient utilization of light due to the high duty-cycle of step scan, good detection performance due to the reduction of stray light, ruggedness due to the small mass of the galvanometer mirror, low cost due to the simplicity of components, and flexibility due to the independent paths for excitation and emission.