gebhart/patrepeval huggingface dataset:
Mapping of <i>Escherichia coli</i> Ribosomal Components Involved in Peptidyl Transferase Activity (1973)

The method of affinity labeling has been used to identify protein components of 50S ribosomal subunits involved in peptidyl transferase activity. E. coli 50S ribosomal subunits were mapped by reaction with the N-bromoacetyl analog of chloramphenicol, an antibiotic known to interact specifically with the active center of the enzyme. The synthetic analog competes with chloramphenicol in binding to 50S ribosomal subunits and inhibits peptidyl transferase activity. It attaches covalently to the ribosome under appropriate conditions and causes an irreversible loss in peptidyl transferase activity. The reagent specifically alkylates cysteine residues of proteins L2 and L27.
Biological and molecular variability of human immunodeficiency virus type 2 isolates from The Gambia (1990)

Seven new human immunodeficiency virus type 2 (HIV-2) isolates (CBL-20 to CBL-26) from The Gambia were characterized. Their cytopathogenicity and growth in vitro correlated with the severity of clinical disease. CBL-22 was highly sensitive to neutralization by HIV-2 sera and was cross-neutralized by some HIV-1 sera. These findings, the differing sizes of envelope glycoproteins of individual isolates, and the sequence analysis of amplified regions of the viral DNAs show that these HIV-2 isolates from one geographical region in West Africa exhibit biological and genome variability comparable to that observed for HIV-1.
ChemInform Abstract: Enzymatic Reactivity and Antitumor Activity of 1‐(β‐D‐Arabinofuranosyl)‐2‐thiocytosine Derivatives. (2000)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Experimental Investigation of Aerodynamics of Flapping-Wing Micro-Air-Vehicle by Force and Flow-Field Measurements (2015)

This study explores the aerodynamic characteristics of a flapping-wing micro aerial vehicle (MAV) in hovering configuration by means of force and flowfield measurements. The effects of flapping frequency and wing geometry on force generation were examined using a miniature six-component force sensor. Additional high-speed imaging allowed identification of the notable different deformation characteristics of the flexible wings under vacuum condition in comparison to their behavior in air, illustrating the relevance of aeroelastic effects. Flow visualization around the flapping wing by means of planar particle image velocimetry (PIV) measurements revealed the formation, development, and shedding of the vortical structures by the wings during flapping motion, with particular emphasis on the clap-and-fling phase. Further stereoscopic PIV measurements performed in the wake showed a momentum surplus wake induced by the clap-and-fling, indicative of thrust generation. The vortical structures in the wake formed during instroke and outstroke were characterized using a three-dimensional wake reconstruction from the planar measurements.
Cytokine Signatures of Innate and Adaptive Immunity in 17DD Yellow Fever Vaccinated Children and Its Association With the Level of Neutralizing Antibody (2011)

The data demonstrated that the overall cytokine signature was associated with the levels of PRNT antibodies with a balanced innate/adaptive immunity with proinflammatory/regulatory profile as the hallmark of PV-PRNT(MEDIUM⁺), whereas a polarized regulatory response was observed in PV-PRNT⁻ and a prominent proinflammatory signature was the characteristic of PV-PRNT(HIGH⁺).
WebOQL: restructuring documents, databases and Webs (2002)

The widespread use of the Web has originated several new data management problems, such as extracting data from Web pages and making databases accessible from Web browsers, and has renewed the interest in problems that had appeared before in other contexts, such as querying graphs, semistructured data and structured documents. Several systems and languages have been proposed for solving each of these Web data management problems, but none of these systems addresses all the problems from a unified perspective. Many of these problems essentially amount to data restructuring: we have information represented according to a certain structure and we want to construct another representation of (part of it) using a different structure. We present the WebOQL system, which supports a general class of data restructuring operations in the context of the Web. WebOQL synthesizes ideas from query languages for the Web, for semistructured data and for Website restructuring.
Determination of cloud points of nonionic surfactants (1965)

Abstract By use of a specially adapted fluorometer attached to a strip chart recorder, a spectrum or cloud‐o‐gram can be obtained from various types of ethylene oxide condensates (nonionic surfactants). A cloud‐point reproducible to ŷ0.2C can be determined from the cloud‐o‐gram. The cloud‐o‐gram is unique for each classification, and differences can be detected within classifications.
Cloning and Membrane Topology of a P type ATPase from Helicobacter pylori (1996)

Southern blot screening of a genomic Helicobacter pylori library was employed to find a P type ATPase using a mixture of 16 DNA oligonucleotides coding for the DKTGT(I/L)T consensus sequence specific for the phosphorylation site of this family of ATPases. A positive clone, pRH439, was isolated and sequenced. The inserted 3.4-kb H. pylori DNA contained an intact open reading frame encoding a protein of 686 amino acids carrying the consensus sites for phosphorylation and ATP binding. The amino acid sequence exhibits a 25-30% identity with bacterial Cd2+ and Cu2+ ATPases. Genomic Southern blot analysis showed that this ATPase was present in all H. pylori strains examined, whereas it was not detectable in Campylobacter jejuni and other bacteria. The membrane topology of this ATPase was investigated using in vitro transcription/translation of fusion vectors to find signal anchor and/or stop transfer sequences. Eight regions of the H. pylori ATPase acted as signal anchor and/or stop transfer sequences and were ordered pairwise along the polypeptide chain placing the N and C-terminal amino acids in the cytoplasm. These transmembrane segments are contained between positions 73 and 92 (H1), 98 and 125 (H2), 128 and 148 (H3), 149 and 176 (H4), 309 and 327 (H5), 337 and 371 (H6), 637 and 658 (H7), and 659 and 685 (H8). The membrane domain of the ATPase, therefore, consists of at least four pairs of transmembrane segments with the phosphorylation site and ATP binding domain located in the large cytoplasmic loop between H6 and H7. The cytoplasmic domain contains several histidines and cysteines, perhaps indicative of divalent cation binding sites. There are several charged amino acids (3 Lys, 2 Glu, 2 Asp), predicted to be in the membrane domain mainly in H2, H3, and H4 and a Cys-Pro-Cys putative metal ion site in H6. The extracytoplasmic domain also has several charged amino acids (5 Glu, 1 Asp, 1 Lys, 1 Arg). It is likely that this novel protein is a heavy metal cation transporting ATPase and belongs to a family of P type ATPases containing eight transmembrane segments.
Dataset on gait patterns in degenerative neurological diseases (2017)

We collected the gait parameters and lower limb joint kinematics of patients with three different types of primary degenerative neurological diseases: (i) cerebellar ataxia (19 patients), (ii) hereditary spastic paraparesis (26 patients), and (iii) Parkinson's disease (32 patients). Sixty-five gender-age matched healthy subjects were enrolled as control group. An optoelectronic motion analysis system was used to measure time-distance parameters and lower limb joint kinematics during gait in both patients and healthy controls.
EUV mask simulation for AIMS (2003)

The objective of this paper is to assess how variations of the chief ray angle of the illumination light incident on an EUV multilayer mask as well as the light bandwidth affect the performance of an AIMS EUV tool with respect to CD measurement and defect evaluation. To this end EUV images were simulated with an EUV lithography simulator developed by the Fraunhofer Institute IISB. The simulations were performed for a multilayer mask with a buried defect under an isolated line. The specifics of the AIMS EUV were taken into account by a superposition of aerial images obtained for different wavelengths. The presentation discusses the simulations and their results.
Spontaneous Phase Anti-correlations in Raman Optical Frequency Comb Generation (2010)

We theoretically investigate and experimentally demonstrate the spontaneous phase anti-correlation between Stokes and anti-Stokes sidebands in Raman frequency comb generation. This anti-correlation suggests a new way to stabilize the comb and synthesize sub-femtosecond pulse trains.
Scavenger Receptor-Mediated Uptake of Fractionated [3H]Heparin in Rat Peritoneal Macrophages. (2000)

We characterized the uptake of fractionated [3H]heparin in rat peritoneal macrophages by investigating the influence of inhibitors of transport and by analyzing binding and internalization processes. Several ligands of scavenger receptors inhibited the binding and subsequent internalization. When temperature was lowered from 37°C to 4°C, the internalization was significantly reduced, while the binding was unchanged, as expected for receptor-mediated endocytosis. However, known inhibitors of receptor-mediated and adsorptive endocytosis of polypeptides and phagocytosis did not affect the uptake. These characteristics are similar to those observed in rat Kupffer cells in our previous study and suggest the involvement of scavenger receptors in the uptake of fractionated [3H]heparin. Finally, although the dissociation constant (Kd) in the macrophage was comparable with that in the Kupffer cell, the internalization rate constant (kint, app) was approximately two-fold larger in the former case, suggesting that the functional regulations of scavenger receptors may differ between these cells.
A strictly anaerobic betaproteobacterium<i>Georgfuchsia toluolica</i>gen. nov., sp. nov. degrades aromatic compounds with Fe(III), Mn(IV) or nitrate as an electron acceptor (2009)

A bacterium (strain G5G6) that grows anaerobically with toluene was isolated from a polluted aquifer (Banisveld, the Netherlands). The bacterium uses Fe(III), Mn(IV) and nitrate as terminal electron acceptors for growth on aromatic compounds. The bacterium does not grow on sugars, lactate or acetate. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain G5G6 belonged to the Betaproteobacteria. Its closest, but only distantly related, cultured relative is Sterolibacterium denitrificans Chol-1S(T) (94.6% similarity of the 16S rRNA genes), a cholesterol-oxidizing, denitrifying bacterium. Strain G5G6 possesses the benzylsuccinate synthase A (bssA) gene encoding the alpha-subunit of Bss, which catalyzes the first step in anaerobic toluene degradation. The deduced BssA amino acid sequence is closely related to those of Azoarcus and Thauera species, which also belong to the Betaproteobacteria. Strain G5G6 is the first toluene-degrading, iron-reducing bacterium that does not belong to the Geobacteraceae within the Deltaproteobacteria. Based on phylogenetic and physiological comparison, strain G5G6 could not be assigned to a described species. Therefore, strain G5G6 (DSMZ 19032(T)=JCM 14632(T)) is a novel taxon of the Betaproteobacteria. We propose the name Georgfuchsia toluolica gen. nov., sp. nov.
Tamoxifen-induced tumor stimulation and withdrawal response. (1980)

A postmenopausal breast cancer patient with a measurable lung metastasis was treated with tamoxifen. The tumor doubling time of the pulmonary lesion was 140 days during spontaneous growth and 52 days during tamoxifen therapy, an almost threefold increase in tumor growth rate. This stimulation persisted fro 1 month after discontinuation of tamoxifen and was followed thereafter by a withdrawal regression lasting greater than 6 months. Awareness of the possible tumor growth enhancement with tamoxifen is of importance especially in view of its prolonged activity. Hormone withdrawal regression may occur following tamoxifen therapy, and a sufficient time interval should therefore elapse between cessation of tamoxifen and initiation of other therapeutic modalities.
Safety Committee (1990)

Journal Article Safety Committee Get access Mark G Torchia Mark G Torchia Chairman University of Manitoba, St. Boniface Research Center, Winnipeg, Manitoba R2H 2A6, Canada Search for other works by this author on: Oxford Academic Google Scholar Journal of Association of Official Analytical Chemists, Volume 73, Issue 1, 1 January 1990, Page 187, https://doi.org/10.1093/jaoac/73.1.187 Published: 14 January 2020
Analyse der Branchenstrukturen (1994)

Für die Untersuchung von Ursachen und Wirkungen der Zusammenschlüsse von Banken und Versicherungen ist eine vorherige Analyse der Branchenstrukturen bzw. der Wettbewerbssituationen notwendig. Sie dient als Grundlage zur späteren Erklärung von Wettbewerbs- und Angebotsveränderungen und deckt gleichzeitig erste mögliche Ursachen der Zusammenschlüsse auf. Die Untersuchung folgt dem Modell der Branchenstrukturanalyse von Porter. 1 Die Wettbewerbskräfte, die die Wettbewerbssituation einer Branche bestimmen, sind in Abb. 4 abgebildet.
Dermal Aspects of Chemical Warfare Agents (2007)

This chapter contains sections titled: Introduction Chemical Warfare Agents of Relevance Basic Skin Structure and Function Principles of Skin Absorption Some Factors Affecting the Percutaneous Absorption of CW Agents Strategies to Mitigate Skin Absorption Summary Acknowledgements References
(Invited) Reliability of Through Silicon Via Technologies (2010)

A lot of technologies are suggested to build up 3-dimensional integrated systems. Due to the relatively short history of 3D-integration attempts most of these technologies are still far away of being mature modules. After a period of time with public relation for 3D-integration by presenting new technological results, now it is necessary to turn attention towards practicability and value for products. The key word is reliability
A method for predicting the throughput characteristics of rate-adaptive wireless LANs (2005)

Rate-adaptive wireless local area networks (WLANs) are fast becoming the networking method of choice. Naturally, there is interest in developing simple and accurate tools for predicting the throughput characteristics of such systems. We present a generic methodology to this end. The method combines a limited set of measurements made using the WLAN device, physical (PHY) layer simulation results, and medium access control (MAC) layer analysis. We consider the IEEE 802.11a standard to show that the methodology we develop predicts throughput characteristics that match closely those from measurements, for typical WLAN environments.
Calcium dobesilate reduces VEGF signaling by interfering with heparan sulfate binding site and protects from vascular complications in diabetic mice (2019)

Abstract Inhibiting vascular endothelial growth factor (VEGF) is a therapeutic option in diabetic microangiopathy. However, VEGF is needed at physiological concentrations to maintain glomerular integrity; complete VEGF blockade has deleterious effects on glomerular structure and function. Anti-VEGF therapy in diabetes raises the challenge of reducing VEGF-induced pathology without accelerating endothelial cell injury. Heparan sulfate (HS) can act as a co-receptor for VEGF. Calcium dobesilate (CaD) is a small molecule with vasoprotective properties that has been used for the treatment of diabetic microangiopathy. Preliminary evidence suggests that CaD interferes with HS binding sites of fibroblast growth factor. We therefore tested the hypotheses that (1) CaD inhibits VEGF signaling in endothelial cells, (2) that this effect is mediated via interference between CaD and HS, and (3) that CaD ameliorates diabetic nephropathy in a streptozotocin-induced diabetic mouse model by VEGF inhibition. We found that CaD significantly inhibited VEGF 165 -induced endothelial cell migration, proliferation, and permeability. CaD significantly inhibited VEGF 165 -induced phosphorylation of VEGFR-2 and suppressed the activity of VEGFR-2 mediated signaling cascades. The effects of CaD in vitro were abrogated by heparin, suggesting the involvement of heparin-like domain in the interaction with CaD. In addition, VEGF 121 , an isoform which does not bind to heparin, was not inhibited by CaD. By applying proximity ligation assays to endothelial cells, we show inhibition of interaction in situ between HS and VEGF and between VEGF and VEGFR-2. Moreover, CaD reduced VEGF signaling in diabetic kidneys and ameliorated diabetic nephropathy and neuropathy, suggesting CaD as a VEGF inhibitor without the negative effects of complete VEGF blockade and therefore could be useful as a strategy in treating diabetic nephropathy.
Density amplification in laser-assisted protein adsorption by photobleaching (2010)

Spatial distributions of proteins are crucial for development, growth and normal life of organisms. Position of cells in a morphogen gradient determines their differentiation in a specific manner. Neutrophils are the initial responders to bacterial infection or other inflammatory stimuli and have the ability to migrate rapidly up shallow gradients of attractants in vivo. Moreover, for the correct wiring of the nervous system, axonal growth cones detect concentration changes of specific proteins called guidance cues to navigate and reach their targets. Guidance cues can either be chemoattractive or chemorepulsive, and the same protein can act successively as both depending on the time point in development or the simultaneous presence of other molecules. A prerequisite to understand chemotaxis in a precise manner is the availability of a method able to reproduce in vitro the spatial distributions of proteins found in vivo. We recently introduced LAPAP (Laser-assisted protein adsoption by photobleaching), an optical method to produce substrate-bound protein patterns with micron resolution. Here, we present how the amount of protein present on the pattern can be increased by one order of magnitude.
Human but not rat amylin shares neurotoxic properties with Aβ42 in long‐term hippocampal and cortical cultures (2008)

Type 2 diabetes mellitus (DM) and Alzheimer's disease (AD) share epidemiological and biochemical features. Both are characterized by insoluble protein aggregates with a fibrillar conformation--amylin in Type 2 DM pancreatic islets, and Abeta in AD brain. To determine whether amylin shares neurotoxic properties with Abeta, we incubated hippocampal and cortical neurons with Abeta42 and human amylin. Different from non-amyloidogenic rat amylin, both caused a dose-, time- and cell type-specific neurotoxicity supporting the notion of a similar toxic mechanism. Depending on the cell type, this finding is also supported by co-incubation of human amylin and Abeta.
Physicochemical Studies on the Binding of Chemicals with Proteins. II (1964)

The binding ability of crystalline bovine serum albumin was examined with several commonly used sulfonamides; sulfisomidine, sulfathiazole, sulfadimethoxine, sulfaphenazole and sulfisoxazole. The relationship between the amount of sulfonamides bound to bovine serum albumin and concentration of the sulfonamide solution at equilibrium is represented by a Langmuir-type equation. In order to study its mechanism, the binding of sulfamonomethoxine with bovine serum albumin was further examined, and in comparison with that of sulfadimethoxine, the effect of methoxyl group on the binding was discussed. Then the relationship between the charge of bovine serum albumin and its binding capacity for these six sulfonamides was examined and the standard binding affinity of sulfonamides was estimated to be about 6kcal/mole. It was concluded that in the binding of the albumin with sulfonamide, electrostatic force is the most important factor and the dissociation constant of sulfonamide affects the binding.
B-12 Estimation Method of Oxygen Consumption During Short Duration Walking (2014)

Amount of daily physical activity is strongly associated with the prevalence of lifestyle-associated diseases, and thus maintaining a certain level of physical activity is recommended. Amount of physical activity, namely energy expenditure, is commonly estimated from linear regression analysis of oxygen uptake during steady-state exercise of different intensities. Short-duration exercise, mainly walking lasting less than 3 minutes, has been reported to account for over 90% of the daily energy expenditure of healthy adults. In a rodent study that measured the oxygen consumption of rodents running for different durations, the energy cost of exercise was shown to decrease as exercise duration increased, so that the oxygen consumption level asymptotically converged at a steady-state level. Wearable accelerometer-based activity counters have widely been used for physical activity assessment. The estimation of energy expenditure by these devices, however, is based on steady-state exercise of more than 5 minutes, and energy consumption of shorter duration exercise is not commonly considered. Oxygen consumption remains elevated for some period of time after exercise. Then, the estimation of energy expenditure for short duration exercise must include excess post-exercise oxygen consumption (EPOC). The amount of EPOC change in duration exercise. Therefore, we attempted to elucidate the energy expenditure for VO_2 kinetics. Healthy men (age 20-40) walked for 1, 3 and 5 minutes on a treadmill at constant speed. Oxygen consumption during and post-exercise was measured using a portable gas analyzer in breath-by-breath mode. A best-fit exponential equation to estimate oxygen consumption from exercise duration and walking speed was generated. The measured data of 3 subjects not used in generating the formula were assessed for systematic error. Cross-validation of estimated oxygen consumption revealed 3.86(ml/kg/min) RMS, and the results of the Bland-Altman analysis revealed neither fixed nor proportional bias. An equation for the estimation of energy expenditure at shorter durations of exercise was successfully generated. Accumulation of additional data may further improve the equation.
Convenient Synthesis of β-Truxinic Acid <i>via</i> Photodimerization of <i>p</i>-Nitrophenyl Cinnamate in the Crystalline State (1985)

Abstract β-Truxinic acid was synthesized in extremely high yield via a topochemical photodimerization of p-nitrophenyl cinnamate crystals, followed by the hydrolysis of the obtained dimer.
Probing solids through THz spectroscopy: Differentiation of chiral and racemic forms of isostructural and non-isostructural cocrystals (2008)

Cocrystals of theophylline (tp) and racemic and chiral forms of both tartaric acid (tart) and malic acid (mal) were investigated using terahertz time-domain-spectroscopy (THz-TDS). Using X-ray powder diffraction (XRPD) the racemic and chiral malic acid cocrystal forms were found to be nearly isostructural and therefore almost indistinguishable using XRPD. Using THz-TDS clear differences were observed both at room temperature and at 109 K. This was rationalized using lattice energy calculations and attributed to a difference in the conformational strain of the acid groups between the two cocrystal forms.