gebhart/patrepeval huggingface dataset:
Biochemical Characterization of the Human Cyclin-dependent Protein Kinase Activating Kinase (1996)

The activation of cyclin-dependent protein kinases (Cdks) is dependent upon site-specific phosphorylation and dephosphorylation reactions, as well as positive and negative regulatory subunits. The human Cdk-activating protein kinase (Cak1) is itself a Cdc2-related cyclin-dependent protein kinase that associates with cyclin H. The present study utilized specific anti-Cak1 antibodies and immunoaffinity chromatography to identify additional Cak1-associated proteins and potential target substrates. Immunoprecipitation of metabolically labeled human osteosarcoma cells revealed a number of Cak1-associated proteins, including p95, p37 (cyclin H), and a 35-kDa protein that was further characterized herein. Microsequence analysis obtained after limited proteolysis revealed peptide fragments that are similar, but not identical to, human and yeast cyclins, thus identifying p35 as a cyclin-like regulatory subunit. The greatest sequence similarity of human p35 is with Mcs2, a yeast cyclin that is essential for cell cycle progression. Immunoaffinity chromatography performed under nondenaturing conditions afforded the isolation of enzymatically active Cak1 from cell lysates, enabling studies of kinase autophosphorylation and comparative substrate utilization. Immunoaffinity-purified Cak1 phosphorylated monomeric Cdc2 and Cdk2, but not Cdk4; the phosphorylation of both Cdc2 and Cdk2 were increased in the presence of recombinant cyclin A. These studies indicate that the Cak1 catalytic subunit, like Cdc2 and Cdk2, associates with multiple regulatory partners and suggests that subunit composition may be an important determinant of this multifunctional enzyme.
Metabolite Analogs. VIII. Syntheses of Some Imidazopyridines and Pyridotriazoles (1957)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMetabolite Analogs. VIII. Syntheses of Some Imidazopyridines and PyridotriazolesHarold Graboyes and Allan R. DayCite this: J. Am. Chem. Soc. 1957, 79, 24, 6421–6426Publication Date (Print):December 1, 1957Publication History Published online1 May 2002Published inissue 1 December 1957https://pubs.acs.org/doi/10.1021/ja01581a018https://doi.org/10.1021/ja01581a018research-articleACS PublicationsRequest reuse permissionsArticle Views639Altmetric-Citations38LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Picosecond ultrasonic study of phonon reflection from solid-liquid interfaces (1997)

We have studied the reflection of longitudinal-acoustic phonons with frequencies between 100 and 300 GHz from solid-liquid interfaces using a picosecond ultrasonic technique. Acoustic phonons were generated by absorption of a picosecond light pulse in a metal film. After propagating through a dielectric layer, the phonons were reflected at the solid-liquid interface. We have measured the reflection coefficient at a series of interfaces between dielectric solids and organic liquids for temperatures from 265 to 300 K. The reflection of 205 GHz phonons was also measured between aluminum and liquid argon and nitrogen. The experimental results were compared with the predictions of the classical acoustic-mismatch model.
Bottlebrush dendritic endings and large dendritic fields: Motion‐detecting neurons in the tectofugal pathway (1998)

In avian and mammalian brains, visual information from the retina is conveyed to the telencephalon via two separate pathways: the thalamofugal and the tectofugal pathways. Recently, Karten et al. ([1997] J. Comp. Neurol. 387:449–465) examined a portion of the tectofugal pathway, the projection from the optic tectum to the nucleus rotundus thalami, in pigeons. They defined two distinct subpopulations of tectal neurons projecting from the stratum griseum centrale (SGC; tectal layer 13) to specific divisions of the rotundus. The goal of this study in chick was to verify the existence of the type I and type II SGC neurons, as defined by Karten et al., and then examine in greater detail the connectivity and morphology of these SGC neurons. Furthermore, our results suggest how the unique morphological characteristics of SGC neurons contribute to the large receptive fields (20–50°) found in physiological recordings and the SGC neuronal response to extremely small (ca. 0.05°), fast-moving (100°/second) stimuli. By injecting retrograde tracer into various divisions of the chick rotundus, we verified that, indeed, the chick did possess type I and type II SGC neurons, as well as a “new” type of SGC neuron, type III, that is not found in the pigeon. We then used intracellular cell-filling techniques to define further these three types of SGC neurons. Our examination revealed the following: Type I SGC neurons had large, circular dendritic fields (average diameter, 1,725 μm) composed of smooth dendrites and ending in spine-rich, bottlebrush endings located in retinorecipient tectal layer 5b; type II SGC neurons had elliptical dendritic fields (average 1,447 μm) and dendritic endings located never more superficially than tectal layer 8; and type III SGC neurons had large dendritic fields (average 1,800 μm) of unknown shape and bottlebrush dendritic endings located in retinorecipient tectal layer 4. We suggest that the neuronal features of the SGC neurons (i.e., bottlebrush dendritic endings and large dendritic fields) are key morphological characteristics for the detection of motion within the tectofugal pathway. Furthermore, because neurons with similar morphology have also been found in the tecta of both mammals and reptiles, we suggest that these neuronal features are fundamental components of a phylogenetically conserved system used for the “extrastriate” detection of motion in vertebrates. J. Comp. Neurol. 396:399–414, 1998. © 1998 Wiley-Liss, Inc.
Aboa Centre for Economics (2008)

Talk about global democracy seems to be fixated on a Reform-Act model of democracy, with 'one person one vote for all affected by the decisions' as for example in a second popularly-apportioned chamber of UN. Politically, that seems wildly unrealistic. But remember that the Reform Acts came very late in process of democratization domestically. The first steps in the beginning that eventually led to full democratization of that sort were: a) limiting the arbitrary rule on the part of the sovereign; and (b) making the sovereign accountable to others (initially a limited set of others, which then expanded). Globally, there are moves afoot globally in both those directions. And once those pieces are in place, there are good reasons for expecting the circle of accountability basically only to expand and virtually never to contract. JEL Classification: F53, F55, H11, K33, N40
Soil Organic Matter and Root and Rhizome Responses to Management Strategies in Smooth Bromegrass Pastures (2014)

Soil organic matter (SOM) is a key component of pasture production. This study investigated how management strategies that varied amount and form of N input in a long‐term experiment affected concentrations and stocks of total‐soil organic C and N, particulate organic carbon (POC), particulate organic nitrogen (PON), root and rhizome mass, C and N contents in topsoil of smooth bromegrass ( Bromus inermis Leyss.) pastures with silty clay loam soils in a wet (2010) and dry (2012) year. Management strategies included: (i) unfertilized pasture grazed with unsupplemented beef cattle (CONT); (ii) unfertilized pasture grazed with dried distillers grains plus solubles (DDGS)‐supplemented beef cattle (SUPP); and (iii) nitrogen‐fertilized pasture grazed with unsupplemented beef cattle (FERT). After 8 yr, management strategies had similar concentrations and stocks of total‐soil organic C and N, POC, and PON, and there were no management strategy × year interactions. From 2010 to 2012, total‐soil organic C and N, POC, and PON stocks increased as soils dried and soil bulk density increased. The CONT and SUPP management strategies had less root and rhizome mass (concentrations and stocks) and greater soil bulk density than FERT. These belowground responses were consistent with earlier research conducted at the site demonstrating greater herbage accumulation and litter deposition in FERT. Management strategies that vary amount and form of N inputs into pasture appear to have low potential to affect total‐soil organic C and N concentrations in the short‐term, but long‐term effects of less root and rhizome contents remain unknown.
Hybrid Organic–Inorganic Inhibitors of a PDZ Interaction that Regulates the Endocytic Fate of CFTR (2012)

Gemeinsam stark: Die kooperative Bindung organischer (rot im Bild) und anorganischer Fragmente ist eine Strategie zur wirksamen Inhibierung von Protein-Protein-Wechselwirkungen. Die Affinität andernfalls schwacher Liganden wird durch die gezielte Koordination eines Rhodium(II)-Zentrums an definierte Lewis-basische Seitenketten in der Umgebung der Proteinbindungsstelle verbessert. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
The American Academy of Orthopaedic Surgeons Appropriate Use Criteria on the Non-Arthroplasty Treatment of Osteoarthritis of the Knee (2014)

Sanders, James O. MD; Heggeness, Michael H. MD; Murray, Jayson MA; Pezold, Ryan MA; Donnelly, Patrick MA; Members of the Writing, Review, and Voting Panels of the AUC on the Non-Arthroplasty Treatment of Osteoarthritis of the Knee
Regionally selective activation and differential regulation of ERK, JNK and p38 MAP kinase signalling pathway by protein kinase C in mood modulation (2011)

A growing body of evidence indicates that the extracellular signal-regulated kinase (ERK) pathway may participate in the neuronal modulation of depression. p38MAPK and c-Jun-N-terminal kinase/stress-activated protein kinase (JNK/SAPK) also belong to the MAPK family which mainly function as mediators of cellular stresses. Since increasing evidence implicates stress as an important factor in vulnerability to depressive illnesses, the involvement of ERK, JNK and p38MAPK pathways in the modulation of mood was investigated in the forced swim test (FST) and tail suspension test (TST). The effect produced by a single acute session of FST and TST on hippocampal and cortical MAPK expression and phosphorylation was investigated by immunoblotting experiments. In the hippocampus of animals exposed to FST and TST, an intensive, PKC-dependent, ERK1, ERK2, JNK, and p38MAPK phosphorylation was observed. In the frontal cortex, the FST and TST produced a PKC-dependent increase of ERK2 and p38MAPK phosphorylation, a PKC-independent activation of JNK and cAMP response element-binding protein (CREB) whereas any involvement of ERK1 was detected. The PKC blocker calphostin C (0.05-0.1 μg i.c.v.), the MEK inhibitor U0126 (10-20 μg i.c.v.), the p38MAPK inhibitor SB203580 (5-20 μg i.c.v.) and the JNK inhibitor II (0.5-5 μg i.c.v.), produced antidepressant-like behaviour without altering locomotor activity. These results illustrate a differentially mediated activation of MAPK in hippocampus and frontal cortex of animals exposed to behavioural despair paradigms. An antidepressant-like phenotype produced by acute blockade of MAPK signalling was also demonstrated.
Reconfigurable Computing: The Theory and Practice of FPGA-Based Computation (2007)

Reconfigurable Computing marks a revolutionary and hot topic that bridges the gap between the separate worlds of hardware and software design- the key feature of reconfigurable computing is its groundbreaking ability to perform computations in hardware to increase performance while retaining the flexibility of a software solution. Reconfigurable computers serve as affordable, fast, and accurate tools for developing designs ranging from single chip architectures to multi-chip and embedded systems. Scott Hauck and Andre DeHon have assembled a group of the key experts in the fields of both hardwa
Student-Built 16mm Continuous Positive Film Processor (1959)

This paper is designed to inform members of the Society of the capabilities of students to actively function within the motion-picture field. The paper concerns the uniqueness, description and functions of a positive, black-and-white film processor built by University of Miami motion-picture students. The machine is capable of operating normally at 100 ft/min, and utilizes glass fiber, polyester resins and synthetic plastics throughout its construction. The processor is now in use for all University positive and soundtrack processing.
Nichtinvasive Quantifizierung der alpha-v-beta-3-Expression in Tumorpatienten mittels PET: Kinetik, Biodistribution und Dosimetrie von [18F]Galacto-RGD (2005)

Ziele: Das Integrin alpha-v-beta-3 spielt eine wichtige Rolle bei der Metastasierung und Angioneogenese. Im folgenden beschreiben wir die Pharmakokinetik und Dosimetrie des alpha-v-beta-3-selektiven PET-Tracers [18F]GalactoRGD. Methode: 19 Patienten mit Melanomen (n=7) oder malignen muskuloskelettalen Tumoren (n=12) wurden nach Injektion von 133–199 MBq [18F]GalactoRGD untersucht. Es erfolgten 3 statische Scans des Körperstammes zwischen 5–100min p.i. (n=13), oder ein dynamischer Scan über 60min, gefolgt von einem statischen Scan (n=6). Zeit-Aktivitäts-Kurven und SUVs wurden durch „Region-of-interest“-basierte Auswertung der Datensätze ermittelt. Für die Beschreibung der Tracerkinetik wurde ein 2- bzw. 3-Kompartiment-Modell verwendet. Die Dosimetrie erfolgte anhand des MIRDOSE 3.0 Programmes. Ergebnis: [18F]GalactoRGD zeigte eine schnelle, überwiegend renale Elimination mit einer biexponentiellen Abnahme der Aktivität aus dem Blutpool (Halbwertszeiten 0.36±0.28 und 34.13±17.15min). SUVs 72min. p.i. waren am höchsten in der Niere (SUV 5.2±3.2), gefolgt von Leber, Milz und Darm (SUVs 2.7±0.7/2.5±0.5/2.2±0.7) mit nur geringer Hintergrundaktivität im Muskel (SUV 0.4±0.08). Die Anreicherung in Tumoren zeigte eine große Varianz (SUV 1.2–9.0) mit im Zeitverlauf ansteigendem Tumor/Blut-Verhältnis (3.3±2.2 72min p.i.). Die Tracerkinetik in Tumoren konnte gut mit einem 3-Kompartiment-Modell beschrieben werden (Variationskoeffizient Vk 15.4–16.2%), während für Hintergrundgewebe die beste Beschreibung mit einem 2-Kompartiment-Modell gelang (Vk 12.3–29.3%). Die effektive Dosis betrug 0.018±0.003 mSv/MBq. Die höchste Organdosis wurde in der Blase (0.20±0.04 mGy/MBq) gefunden. Schlussfolgerung: [18F]GalactoRGD zeigt eine günstige Biodistribution und geringe Strahlenbelastung für den Patienten. Die Tracerkinetik in Tumoren weist auf eine spezifische, reversible Rezeptorbindung hin. Damit ist [18F]GalactoRGD für eine Darstellung und Quantifizierung der alpha-v-beta-3-Expression in Patienten sehr gut geeignet.
Symmetrical Peripheral Gangrene (1974)

<i>To the Editor.</i>–Symmetrical peripheral gangrene as a complication of hypotension and poor tissue perfusion was presented by Goodwin and Berne in the JuneArchives(108:780, 1974). We agree entirely with the authors' conclusions, particularly the need to recognize the syndrome, thereby avoiding unnecessary angiography and surgical intervention. However, we wish to emphasize that symmetry is not an absolute prerequisite for the diagnosis of non-occlusive ischemia of the extremities. We have recently described our experience with asymmetrical non-occlusive ischemia of the lower extremities.<sup>1</sup>In all instances, the patient was admitted with the classic findings of unilateral embolic or thrombotic occlusion of a major limb vessel. Angiography was performed in these cases and showed complete patency of the arteries. However, the rate of filling and flow through the peripheral arterial system was extremely slow. In one of our cases, Doppler flowmeter recordings were helpful in that a flat tracing was
Fluorene Functionalized Nanoporous SBA-15 as a Novel Adsorbent for Fast and Efficient Removal of Acid Dyes (2018)

SBA-15 grafted with N-(4-(trimethylsilyl) butyl)-9H-fluorene-9-amine was synthesized by a simplestrategy and applied to removal of Acid Red 33 and Acid Black 194 from aqueous solutions. SBA-15 was synthesized and Functionalized according to procedure in the literature. Fluorenefunctionalized SBA-15 was showed the BET surface area 200 m2g-1 and pore diameter 4.2 nm,based on adsorption-desorption of N2 at 77 K. The presence of organic groups in the silicaframework was demonstrated by FTIR and Raman spectrum. The effects of pH, amount ofadsorbent, contact time and dye concentration on adsorption were determined in order to find theoptimum adsorption conditions. The data fitted well to the Langmuir model with maximumadsorption capacities 500.0 mg/g for Acid Red 33 and 110.0 mg/g for Acid Black 194. The resultswere shown that this methodology could be suitable for the removal of the pollutant acid dyes fromdyeing wastewater of handmade carpet’s fibers.
Stochastic Analysis of Monthly Precipitation Data in Alabama (1998)

ABSTRACT As an assessment method in the protection of the environment, a stochastic approach is used to analyze the monthly precipitation data in Alabama. Monthly mean precipitation data from 12 stations in discrete equidistant form were analyzed. The computed precipitation trends for the decade 1986-1995 showed 10 stations with positive trends ranging from +0.62 mm to +4.06 mm per year, while the other two stations showed negative trends. For the three decades studied only two stations, Mobile and Huntsville, show consistently positive trends, predicting more rainwater to recharge the local aquifers and more incidences of floods in these areas. For the periodicity, the power spectrum showed that the periodic components tend to be insignificant in Alabama, with yearly oscillations contributing less than 30% to the periodicity. The auto-correlogram showed that the precipitation of each month does not depend on that of the previous month, indicating a variable monthly precipitation behavior in Alabama.
Impedance Matching for RFID Tag Antennas (2008)

Passive UHF RFID tag consists of a microchip attached to an antenna. Proper impedance match between the antenna and the chip is crucial in RFID tag design. It directly influences RFID tag performance characteristics such as the read range. It is known that an RFID microchip is a nonlinear load whose complex impedance varies with the frequency and the input power. We investigate the effect of using constant chip impedance while designing UHF RFID tags for specific frequencies. Commercial Avery Dennison AD-220 tag was simulated by Ansoft HFSS software. Afterwards, the read range of the tag was obtained numerically and compared with the measured values using the Voyantic Tagformance RFID measurements system.
Mass spectrometry in structural and stereochemical problems. CCLVI—electron impact induced fragmentation of juvenile hormone analogs (1976)

Abstract Complete high resolution mass spectra of several analogs of the juvenile hormones have been analyzed with the aid of our recently developed INTSUM program and mass spectra of deuterated analogs. Representative compounds chosen for study included the biologically interesting (2E, 4E)‐3,7,11‐trimethyl‐2,4‐dodecadienoates (1,2), (2E,4E)‐11‐methoxy‐3,7,‐11‐trimethyl‐2,4‐do‐decadienoates (3,4) and three analogs (5‐7) of the trans‐trans‐farnesoate skeleton. These compounds display characteristic fragmentations which can be used to detect the presence of the compounds in complex mixtures using mass fragmentography or aid in the structure elucidation of related compounds.
Indium Phosphide MEMS Cantilever Resonator Sensors Utilizing a Pentacene Absorption Layer (2008)

We report a microelectromechanical system cantilever waveguide resonator sensing platform utilizing a novel optical readout scheme and the organic semiconductor pentacene as a surface absorbing layer. In this paper, the measurement of isopropyl alcohol and ethanol vapors by way of mass induced frequency shift using a cantilever microbalance is demonstrated. Vapor was introduced to the system through a custom built environmental chamber. A frequency shift due to a mass absorption of 65 Hz was measured, corresponding to a measurement of 6.92 ±1.1 ×10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-14</sup> g with a minimum detectable mass of 5.09 ×10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-15</sup> g for the devices presented. The pentacene absorbing layer in this paper shows it for the first time, functioning as a mass absorbing layer. These results are also the first demonstration of repeatable mass sensing performed using the integrated indium phosphide cantilever waveguide sensor platform.
Methylation Dependent Functional Switch Mechanism of the Escherichia coli Ada Protein. (1995)

NMR studies have revealed the mechanism of a functional switch in the Escherichia coli Ada protein, which converts the DNA methyltransferase to a transcriptional regulator. The Ada protein acquires its ability to bind specific DNA sequences after an irreversible methyl transfer to its own cysteine residue (Cys69) from methyl-phosphotriesters within the alkylated DNA. NMR analysis have revealed that the segment from residues 102 to 123 forms a helix-turn-helix structure, and a site-directed mutagenesis study has identified the second helix to play a crucial role in specific recognition of DNA. NMR experiments on the methylated protein-DNA complex showed that although the major contacts are made by residues within the recognition helix, the S-methyl group at Cys69 come into direct contact with the cognate DNA. Therefore, the direct contact of this region after methylation is the “switch” which converts the Ada protein from a nonspecific DNA binding form to a transcription factor.
Simultaneous red–green–blue reflection and wavelength tuning from an achiral liquid crystal and a polymer template (2009)

A broad and fast wavelength tuning of the photonic bandgap of a hybrid photonic band structure (PBS) formed from an achiral nematic liquid crystal and a polymer template. Remarkably, red-green-blue reflection from a single device and a single achiral nematic liquid crystal mixture is reported without any additional optical components such as a color filter.