gebhart/patrepeval huggingface dataset:
Active and inactive genes localize preferentially in the periphery of chromosome territories. (1996)

The intranuclear position of a set of genes was analyzed with respect to the territories occupied by the whole chromosomes in which these genes are localized. Genes and their respective chromosome territories were simultaneously visualized in three-dimensionally preserved nuclei applying dual color fluorescence in situ hybridization. Three coding (DMD, MYH7, and HBB) and two noncoding sequences (D1Z2 and an anonymous sequence) were analyzed in four different cell types, including cells where DMD and MYH7 are actively transcribed. Spatial analysis by confocal laser scanning microscopy revealed that the genes are preferentially located in the periphery of chromosome territories. This positioning was independent from the activity of the genes. In contrast, the non-expressed anonymous fragment was found randomly distributed or localized preferentially in the interior of the corresponding chromosome territory. Furthermore, the distribution of the analyzed genes within the territorial peripheries was found to be highly characteristic for each gene, and, again, independent from its expression. The impact of these findings with regard to the three-dimensional arrangement of the linear DNA string within chromosome territories, as well as with respect to a putative nuclear subcompartment confining gene expression, are discussed.
Apoptosis Is the Essential Target of Selective Pressure against p53, whereas Loss of Additional p53 Functions Facilitates Carcinoma Progression (2011)

The high frequency of p53 mutation in human cancers indicates the important role of p53 in suppressing tumorigenesis. It is well established that the p53 regulates multiple, distinct cellular functions such as cell-cycle arrest and apoptosis. Despite intensive studies, little is known about which function is essential, or if multiple pathways are required, for p53-dependent tumor suppression in vivo. Using a mouse brain carcinoma model that shows high selective pressure for p53 inactivation, we found that even partially abolishing p53-dependent apoptosis by Bax inactivation was sufficient to significantly reduce the selective pressure for p53 loss. This finding is consistent with previous reports that apoptosis is the primary p53 function selected against during Eμ-myc-induced mouse lymphoma progression. However, unlike observed in the Eμ-myc-induced lymphoma model, attenuation of apoptosis is not sufficient to phenocopy the aggressive tumor progression associated with complete loss of p53 activity. We conclude that apoptosis is the primary tumor suppressive p53 function and the ablation of additional p53 pleiotropic effects further exacerbates tumor progression.
Serotonergic Function in Obsessive-Compulsive Disorder (1992)

To evaluate serotonergic (5-hydroxytryptamine) function in obsessive-compulsive disorder, behavioral and neuroendocrine responses to m-chlorophenylpiperazine (m-CPP; 0.5 mg/kg orally) and fenfluramine hydrochloride (60 mg orally) were examined in 20 patients and 10 healthy controls under double-blind, placebo-controlled conditions. Following m-CPP, but not fenfluramine or placebo, 55% (11/20) of the patients with obsessive-compulsive disorder experienced a transient exacerbation of obsessive-compulsive disorder. Prolactin response was blunted in patients following m-CPP but not following fenfluramine. Patients with greater behavioral response to m-CPP had smaller prolactin responses. Cortisol response to m-CPP and fenfluramine did not significantly differ between the groups. Behavioral and neuroendocrine responses appeared divergent. This does not suggest simply upregulation or downregulation of 5-hydroxytryptamine receptors, but rather complex mechanisms involving multiple neurotransmitter and neuromodulator systems.
Designing and Characterizing Metalenses for the Increased Light Extraction of MWIR LEDs (2019)

High radiance IRLEDs have been achieved, but much of the light created still remains in the device resulting in poor wall-plug efficiencies. Preliminary results presented here show increased light extraction by the addition of a 2D lens to the device, known as a metalens.
Development and antimicrobial susceptibility studies of in vitro monomicrobial and polymicrobial biofilm models with Aspergillus fumigatus and Pseudomonas aeruginosa (2014)

Simultaneous static coculture of A. fumigatus sporelings grown for 12 h or longer was superior to ungerminated conidia with P. aeruginosa for the development of A. fumigatus-P. aeruginosa biofilm. P. aeruginosa-A. fumigatus polymicrobial biofilm shows differential susceptibility to antimicrobial drugs whereas the susceptibility of A. fumigatus to antimicrobial drugs was unchanged.
Iodide reduction of sulfilimines. Evidence for the partitioning of sulfurane intermediates (1985)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTIodide reduction of sulfilimines. Evidence for the partitioning of sulfurane intermediatesPaul R. Young and Patrick E. McMahonCite this: J. Am. Chem. Soc. 1985, 107, 25, 7572–7577Publication Date (Print):December 1, 1985Publication History Published online1 May 2002Published inissue 1 December 1985https://pubs.acs.org/doi/10.1021/ja00311a058https://doi.org/10.1021/ja00311a058research-articleACS PublicationsRequest reuse permissionsArticle Views187Altmetric-Citations4LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Effect of Insulin on Farnesyltransferase (1998)

We have previously demonstrated that insulin activates farnesyltransferase (FTase) and augments the amounts of farnesylated p21 (Goalstone, M. L., and Draznin, B. (1996) J. Biol. Chem. 271, 27585-27589). We postulated that this aspect of insulin action might explain the "priming effect" of insulin on the cellular response to other growth factors. In the present study, we show the specificity of the effect of insulin on FTase. Insulin, but not insulin-like growth factor-1 (IGF-1), epidermal growth factor (EGF), or platelet-derived growth factor (PDGF), stimulated the phosphorylation of the alpha-subunit of FTase and the amounts of farnesylated p21. Even though all four growth factors utilized the Ras pathway to stimulate DNA synthesis, only insulin used this pathway to influence FTase. Insulin failed to stimulate FTase in cells expressing the chimeric insulin/IGF-1 receptor and in cells derived from the insulin receptor knock-out animals. Insulin potentiated the effects of IGF-1, EGF, and PDGF on DNA synthesis in cells expressing the wild type insulin receptor, but this potentiation was inhibited in the presence of the FTase inhibitor, alpha-hydroxyfarnesylphosphonic acid. We conclude that the effect of insulin on FTase is specific, requires the presence of an intact insulin receptor, and serves as a conduit for the "priming" influence of insulin on the nuclear effects of other growth factors.
Tumor suppressor p53 Arg72Pro polymorphism and longevity, cancer survival, and risk of cancer in the general population (2007)

p53 is an important tumor suppressor, normally preventing cancer development via apoptosis. A genomic Arg72Pro substitution in the p53 protein has important influence on cell death via apoptosis, which could be beneficial. We therefore tested the hypotheses that this polymorphism influences longevity, survival after a cancer diagnosis, and risk of cancer in the general population. We examined a cohort of 9,219 participants ages 20-95 from the Danish general population with 100% follow-up. The overall 12-yr survival was increased in p53 Arg/Pro heterozygotes with 3% (P = 0.003) and in Pro/Pro homozygotes with 6% (P = 0.002) versus Arg/Arg homozygotes, corresponding to an increase in median survival of 3 yr for Pro/Pro versus Arg/Arg homozygotes. We also demonstrated an increased survival after the development of cancer, or even after the development of other life-threatening diseases, for Pro/Pro versus Arg/Arg homozygotes. The Arg72Pro substitution did not associate with decreased risk of cancer. In conclusion, in this large cohort from the general population, we show that a well-known functional single nucleotide polymorphism in the tumor suppressor p53 protein leads to increased longevity, but not to decreased risk of cancer. The increased longevity may be due to increased survival after a diagnosis of cancer or other life-threatening diseases.
Differential Responsiveness of Conduit and Resistance Coronary Arteries to Endothelin A and B Receptor Stimulation in Anesthetized Dogs (1993)

The effects of endothelin-1 (ET-1), an ET(A)/ETB-receptor agonist, and IRL 1620, a potent and selective ETB-receptor agonist, were assessed on left circumflex coronary artery diameter (sonomicrometry) and flow (electromagnetic flow probe) in pentobarbital-anesthetized dogs. Intracoronary (i.c.) bolus injections of ET-1 (80 pmol/dose) caused large, sustained coronary diameter decreases (281 +/- 39 microns) and transient flow increases (5.6 +/- 2.6 ml/min), followed by transient (10.0 +/- 1.9 ml/min) and then sustained flow reductions (6.6 +/- 2.5 ml/min) before terminating in ventricular fibrillation after two to five doses (max delta s; n = 4 dogs). IRL 1620 boluses (5-2,000 pmol/dose i.c.; max delta s; n = 3) also dose-dependently and transiently increased (16.8 +/- 1.4 ml/min; 200 pmol), then transiently decreased (12.8 +/- 1.5 ml/min; 1,600 pmol) flow but had minimal effects on diameter (delta = -23 +/- 4 microns; 2,000 pmol). Doses of IRL 1620 beyond 400 pmol were accompanied by a slowly responding, sustained decrease in baseline flow (-9.2 +/- 2.7 ml/min) and baseline diameter (232 +/- 150 microns). In a separate group of dogs (n = 5), IRL 1620 (400 pmol i.c.) was evaluated before and after sequential inhibition of cyclooxygenase (indomethacin; 10 mg/kg i.v.) and then nitric oxide synthase (N omega-nitro-L-arginine methyl ester, L-NAME; 50 mg/kg i.v.). Indomethacin alone did not affect the flow increase to IRL 1620 (11.0 +/- 2.0 versus 11.8 +/- 1.8 ml/min) but blunted the flow decrease by 30 +/- 6% (10.6 +/- 1.4 versus 7.1 +/- 0.7 ml/min).(ABSTRACT TRUNCATED AT 250 WORDS)
Magnetofection—A highly efficient tool for antisense oligonucleotide delivery in vitro and in vivo (2003)

Delivery of antisense oligodesoxynucleotides (ODN) into primary cells is a specific strategy for research with therapeutic perspectives but transfection-associated difficulties. We established the technique of magnetofection to enhance ODN delivery at low toxicity and procedure time in vitro and in vivo. In vitro, target knockout was assessed at protein and mRNA levels and by measuring superoxide generation after antisense magnetofection against the p22(phox) subunit of endothelial NAD(P)H-oxidase. Under magnetic field guidance, low-dose magnetic particle-bound ODN were transfected to 84% human umbilical vein endothelial cells within 15 min followed by nuclear accumulation within 2 h, which required 24 h using standard methods. Antisense magnetofection against p22(phox) significantly decreased basal and prevented stimulated superoxide release due to loss of NAD(P)H-oxidase activity by mRNA knockout as assessed after 24 h. Knockout of endothelial phosphatase SHP-1 and connexin 37 proteins confirmed the method's efficiency. Transfection-associated toxicity was minimal. Twenty-four hours after injection of fluorescence-labeled ODN into femoral arteries of male mice, there was specific ODN uptake only into cremaster vessels exposed to magnetic fields during injection. Magnetofection is an ideal tool for delivery of functionally active ODN to difficult-to-transfect cells to study gene/protein function and a promising strategy for targeted ODN delivery in vivo.
The Next Step in Biomimetic Material Design: Poly‐LacNAc‐Mediated Reversible Exposure of Extra Cellular Matrix Components (2012)

Herein is demonstrated that a biomimetic, in-vivo-like presentation of extracellular matrix (ECM) proteins such as fibronectin, laminin, and collagen IV mediated by a fully functional poly-sugar (polyLacNAc)–lectin complex can be achieved on a biomaterials surface. Cells recognize and bind to these immobilized proteins and can, due to the reversible protein presentation, remodel initially presented ECM proteins.
STUDIES ON THE EFFECT OF SURAMIN ON CELL-PROLIFERATION OF EPITHELIAL TISSUE RENEWAL IN THE MOUSE (1995)

The effect of a three-week treatment regimen with suramin (30, 60 or 90 mg/kg body weight) on the cell proliferation of small intestinal crypts and dorsal epidermis of the nude mouse was studied using standard auto-radiographic methods after in vivo pulse labeling with H-3-thymidine. Suramin was slightly toxic to the animals in a dose-dependent manner (p<0.01) as measured by the decrease of the rate of weight gain of the treated animals. In the small intestine suramin treatment led to crypt shrinkage (factor: about 70-80%; p<0.05) mainly by increasing the rate of cell loss out of the crypt epithelium (p<0.05) and to a lesser degree by inhibiting the proliferation of crypt cells. However, this suramin treatment scheme did not significantly affect the H-3-thymidine labeling index and the mitotic index of the basal cell layer of the epidermis. The present findings indicate that suramin can be used to approach the problem of differential homeostatic reactions of epithelial tissue renewal in vivo.
Thorakale Aortenstents (2004)

Zusammenfassung Ziel: Darstellung der sinnvollen Indikationen für eine interventionelle Aortenstentgraftimplantation zur Erreichung guter Akut- und Langzeitergebnisse. Patienten, Material und Methode: Daten von 51 Patienten mit thorakalen Aortenaneurysmata, Typ B Dissektionen, gedeckten Perforationen und einer aorto-bronchialen Fistel, die zur interventionellen thorakalen Aortenstentimplantation vorgestellt worden waren, wurden ausgewertet. Alle Patienten wurden interdisziplinär mit der thoraxchirurgischen und radiologischen Klinik besprochen. 20 Patienten wurden primär chirurgisch und 31 primär interventionell behandelt. Bei allen wurde eine Multidetektor-CT-Angiographie durchgeführt. Die Stentgraftimplantation erfolgte in einer DSA-Angiographieeinheit unter Vollnarkose, wobei 29 Patienten eine Talent LPS™- und 4 eine Excluder™-Endoprothese implantiert bekamen. Ergebnisse: 29 Patienten hatten Aneurysmata im Bereich der deszendierenden thorakalen Aorta, 21 Patienten eine Typ-B-Dissektion, 19 eine gedeckte Perforation und 1 Patient eine aortobronchiale Fistel. Der Follow-up-Zeitraum betrug 13,4 Monate. Bei 29 Patienten war die Stentgraftimplantation erfolgreich, bei 2 Patienten ließ sich die Prothese wegen massiver iliakaler Gefäßkinckings und -verkalkungen nicht in die Aorta vorschieben. Bei einem Patienten trat eine iliakale Dissektion auf, die in gleicher Sitzung operativ behandelt wurde. In 2 Fällen zeigte sich in der Kontrolle ein Endoleak, das erfolgreich in einer zweiten Intervention verschlossen wurde. Ein Patient verstarb periinterventionell. Neurologische Komplikationen traten nicht auf. Schlussfolgerung: Die perkutane Behandlung von Läsionen der deszendierenden thorakalen Aorta mittels Stentgraftimplantation ist eine sichere, erfolgversprechende Alternative zur chirurgischen Therapie. Vor Intervention ist eine Multidetektor-CT-Angiographie erforderlich, um die Möglichkeit einer endoluminalen Therapieoption zu prüfen und die Intervention exakt zu planen.
Use of intense pulsed light and a retinyl‐based cream as a potential treatment for cellulite: a pilot study (2006)

IPL treatment with or without a retinyl-based cream can improve the appearance of peau d'orange cellulite, though the cream may augment cosmetic improvement. This approach is well tolerated, has minimal side effects, and is accompanied by a high degree of patient satisfaction.
Epitaxial growth of gallium arsenide prepared by low-pressure metal-organic chemical vapour deposition at low temperatures (1992)

The low-pressure metal-organic chemical vapour deposition (LPMOCVD) technique has been investigated previously as a growth method for compound semiconductors, offering the possibility of selective epitaxy and the potential advantage of better controllability for changing the doping level and the alloy composition. Low-temperature growth is also desirable to reduce the carbon incorporation generated by the decomposition of the organic radicals. In this article we report for the first time the epitaxial growth of gallium arsenide (GaAs) by LPMOCVD at temperatures as low as 510 °C. The vertical reactor that was developed by the authors employs conventional precursors such as trimethylgallium and arsine. By carefully choosing the growth parameters, we were able to grow high-quality GaAs epilayers with good surface morphology at temperatures as low as 510 °C. The carbon incorporation is shown to decrease with decreasing growth temperature without deterioration of the film quality. By carefully controlling the purity of the sources and the gas flow dynamics, we reduced the deep level impurity concentration and obtained reproducible n-type material with residual net donor concentration of 4.4 × 10 14 cm −3 and mobility of 92 000 cm 2 V −1 s −1 at 77 K.
Selektive Trimerisierung vonα-Olefinen mit Triazacyclohexan-Komplexen des Chroms als Katalysatoren (2000)

Olefintrimere können selektiv aus α-Olefinen mit n-Alkyl-substituierten Triazacyclohexan-CrCl3-Komplexen als Katalysatoren in Gegenwart von Methylalumoxan (MAO) synthetisiert werden.
Metal-molecule-semiconductor heterostructures for nano-device applications (2004)

The key challenge for molecular devices is the nature of the microscopic contacts to the active molecules and the need to develop well-controlled interfaces in order to achieve stable low-resistance contacts. When compared to metals, semiconductor contacts can be engineered more controllably and are more practical for device applications. In addition to the molecular energy levels, the energy bands of the semiconductor contact give us an extra degree of freedom for enhanced device functionality. The focus of the present work is on metal-molecule-semiconductor heterostructures in a Au/molecule/GaAs configuration. To begin with, stable monolayers of alkane-monothiols, alkane-dithiols and aromatic-dithiols on heavily doped p-type GaAs were self-assembled. A chemical etching procedure was used to overcome the oxidation of the GaAs surface during SAM formation. FTIR spectra indicate the formation of uniform and crystalline monolayers. Ellipsometry measurements give layer thicknesses that are consistent with calculations. A low-energy indirect path technique is used to evaporate Au on the molecular layer without damaging or penetrating it. I-V measurements on the Au/molecule/GaAs devices indicate a substantial increase in conductivity due to the presence of the molecular layer, which can be treated as a leaky dielectric. The results are consistent with the presence of molecular dipole moments at the interface.
Resolution of Steroid Glucuronides by Thin-Layer Chromatography on Polyamide (1970)

A new procedure for the fractionation of steroid glucuronides by thin-layer chromatography on polyamide is presented. Good results have been obtained with developing systems constituted by mixtures of polar solvents. A suitable reagent for the detection of the glucuronides on polyamide thin-layers is also described. The sensitivity of the precedure is of the order of 0.1 µg.
Random vs Structured Pilot Assignment in Cell-Free Massive MIMO Wireless Networks (2018)

This paper addresses the performance improvement that structured pilot assignment policies can bring about, relative to a random pilot assignment, in cell-free massive MIMO wireless networks. It is shown that structured policies can deliver a multiple-fold reduction in the pilot overhead required to keep pilot contamination at some acceptably low level. While the implementation of the structured policies considered in the paper might require some degree of centralized control, their performance also captures what distributed nonrandom pilot assignment schemes (e.g., greedy or collision- detecting algorithms) can hope to approach.
Sleep difficulties and their impact on recovery following mild traumatic brain injury in children (2016)

Sleep difficulties are common following mild TBI in children and are predictive of longer-term outcomes. Identifying children with sleep difficulties post-injury and providing support to facilitate sleep may improve their longer-term functioning.
Political Economic Markets: PERTs and PESTs in Food and Agriculture (1982)

•. J I lYniversity of California, Berkeley. .JJept. of agricultural and resource economic!l Working Paper ~al ClJDARE __ i. __ ~ _ _ ~ __ ... __ \'lorKing Paper :\0. 231 POLITIC\], ECO\()\lIC ]\L\RXETS: A,\D PESTS I:-J FOOD .L\,\TI ACHICULTURE by PEr~TS Cordon C. Rausser {~jA['.NlNl tt (JUNOt\TlON A riIi:ent St:!ticn Giannini. FOU:1Cl:itiol1of Ar,ricultllrnJ Econo'll i cs July 11)W:
Generation of ultrasonic leaky waves at liquid-solid interfaces (1979)

Recent experimental investigations by M. A. Breazeale, L. Adler, and G. W. Scott [J. Appl. Phys. 48, 530–537 1977)] have verified the theory of Bertoni and Tamir [Appl. Phys. 2, 157–172 (1973)]. Excitation of leaky Rayleigh waves takes place at the liquid-solid interface when a Gaussian ultrasonic beam is incident at or near the Rayleigh angle to the interface. Now we should like to report two additional examples of leaky wave generation: (1) When a thin (less than a wavelength) ceramic layer is added to a metal surface, leaky waves are generated. The velocity and amplitude distribution of these waves will be discussed. (2) For interfaces such as water-Plexiglas no real solution of the leaky Rayleigh velocity exists since the sound velocity in the water is larger than the shear velocity in Plexiglas. By studying the reflection of an incident Gaussian beam from water-Plexiglas interfaces we have observed leaky waves near the longitudinal critical angle. Similar observations were made at water-sediment interfaces. [M. A. Breazeale and L. Bjørnø, Proc. Ultrasonics Int., 1977, pp. 440–444]. [Research supported in part by the Office of Naval Research.]
Scanning Tunneling Microscopy Study of In situ Hydrogenation of Si(110) Surface (2010)

The in situ hydrogenation of the Si(110) surface has been studied by scanning tunneling microscopy. It has been found that a small amount of atomic hydrogen (H) generates missing protrusion defects in the reconstructed “16×2” areas. In the disordered areas, a small amount of H results in the rearrangement of pentagon pairs into a local “4×5” structure after annealing. The saturation coverage and higher temperatures result in a new type of zigzag-like surface structure. The structures formed on Si(110) by in situ hydrogenation are significantly different from those on hydrogenated surfaces obtained by chemical methods, where 1×1 reconstruction is dominant.
Small cell carcinoma of the ovary diagnosed in early pregnancy (1991)

Small cell carcinoma of the ovary is a rare malignancy affecting young women. We report the first case of small cell carcinoma of the ovary and coincident pregnancy. The patient died of the disease 23 months after the initial diagnosis.