gebhart/patrepeval huggingface dataset:
<b><i>Lactobacillus reuteri</i></b> DSM 20016: purification and characterization of a cystathionine γ-lyase and use as adjunct starter in cheesemaking (2002)

A homo-tetrameric approximately 160-kDa cystathionine gamma-lyase was purified to homogeneity from Lactobacillus reuteri DSM 20016 by four chromatographic steps. The activity was pyridoxal-5'-phosphate dependent and the enzyme catalyzed the alpha,gamma-elimination reaction of L-cystathionine, producing L-cysteine, ammonia and alpha-ketobutyrate. The enzyme was active towards a range of amino acids and amino acid derivatives, including methionine. The pH and temperature optima were found to be 8.0 and 35 degrees C. respectively. Isoelectric pH (pI) was approximately 5.0 as determined by two-dimensional electrophoresis. Sensitivity to chemical inhibitors was typical of lactococcal cystathionine gamma- and beta-lyases, except it was inhibited by sulphydryl reagents. The N-terminal sequence was MKFNTQLIHGGNSED, which had 100% homology with cystathionine beta-lyase of Lb. reuteri 104R (Accession Number (CAC05298). Lb. reuteri DSM 20016. together with 10 other strains of non-starter lactic acid bacteria, was used as adjunct starter in the production of miniature ('anestrato Pugliese-like cheeses. After 40 d ripening, the water-soluble extract of the cheeses with added Lactobacillus fermentum DT41 and Lb. reuteri DSM 20016 contained the highest enzyme activities on cystathionine and methionine substrates. Determinations of methanethiol, dimethyl sulphide, dimethyl disulphide and dimethyl trisulphide in the miniature cheeses confirmed the findings of enzyme activities.
Entanglement and four-wave mixing effects in the dissipation-free nonlinear interaction of two photons at a single atom (2003)

We investigate the nonlinear interaction between two photons in a single-input pulse at an atomic two-level nonlinearity. A one-dimensional model for the propagation of light to and from the atom is used to describe the precise spatiotemporal coherence of the two-photon state. It is shown that the interaction generates spatiotemporal entanglement in the output state similar to the entanglement observed in parametric down-conversion. A method of generating photon pairs from coherent pump light using this quantum-mechanical four-wave mixing process is proposed.
CCDC 1588347: Experimental Crystal Structure Determination (2017)

An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
Evidence for Metabolic Provisioning by a Common Invertebrate Endosymbiont, Wolbachia pipientis, during Periods of Nutritional Stress (2009)

Wolbachia are ubiquitous inherited endosymbionts of invertebrates that invade host populations by modifying host reproductive systems. However, some strains lack the ability to impose reproductive modification and yet are still capable of successfully invading host populations. To explain this paradox, theory predicts that such strains should provide a fitness benefit, but to date none has been detected. Recently completed genome sequences of different Wolbachia strains show that these bacteria may have the genetic machinery to influence iron utilization of hosts. Here we show that Wolbachia infection can confer a positive fecundity benefit for Drosophila melanogaster reared on iron-restricted or -overloaded diets. Furthermore, iron levels measured from field-collected flies indicated that nutritional conditions in the field were overall comparable to those of flies reared in the laboratory on restricted diets. These data suggest that Wolbachia may play a previously unrecognized role as nutritional mutualists in insects.
THE STRUCTURE OF ELAIOMYCIN, A TUBERCULOSTATIC ANTIBIOTIC (1956)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTHE STRUCTURE OF ELAIOMYCIN, A TUBERCULOSTATIC ANTIBIOTICC. L. Stevens, B. T. Gillis, J. C. French, and T. H. HaskellCite this: J. Am. Chem. Soc. 1956, 78, 13, 3229–3230Publication Date (Print):July 1, 1956Publication History Published online1 May 2002Published inissue 1 July 1956https://pubs.acs.org/doi/10.1021/ja01594a078https://doi.org/10.1021/ja01594a078research-articleACS PublicationsRequest reuse permissionsArticle Views90Altmetric-Citations28LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Applications of immortalized cells in basic and clinical neurology (1991)

Immortalized cell lines can serve as model systems for studies of neuronal development and restoration of function in models of neurological disease. Cell lines which result from spontaneous or experimentally-induced tumors have been used for these purposes. More recently, the techniques of genetic engineering have resulted in the production of cell lines with specific desired characteristics. This has been accomplished by insertion of a desired gene into a pre-existing immortal cell or by immortalizing primary cells. The production of immortal cell lines using temperature-sensitive immortalizing genes offers an additional method of controlling gene expression, and thereby controlling cell proliferation and differentiation. In the nervous system, these techniques have produced immortal cell lines with neuronal and glial properties.
Structure locale du champ hydrodynamique dans les écoulements dispersés liquide-liquide concentrés (2001)

L'objectif de ce travail est de caractériser la structure du champ hydrodynamique dans deux écoulements dispersés liquide-liquide de type homogène et de zone de mélange, par les mesures locales, simultanées et instantanées des champs de vitesse des deux phases, de la fraction volumique et du diamètre des gouttes. Ces mesures sont menées pour des fractions volumiques de la phase dispersée pouvant atteindre 40%, à l'aide d'une technique originale adaptée de la Vélocimétrie par Images de Particules en milieu optiquement homogène, développée et validée dans le cadre de ce travail. Les phases continue (mélange eau-glycérol) et dispersée (heptane) sont localement différenciées par la présence dans la phase continue d'un traceur fluorescent (rhodamine 6G). La technique est d'abord appliquée à l'étude d'écoulements homogènes co-courants dans l'objectif de caractériser l'influence de la fraction volumique de la phase dispersée sur la force de traînée diphasique et sur la turbulence des deux phases. Les résultats expérimentaux sont comparés à des modèles existants et /ou à des données de la littérature. Des éléments intéressants concernant la structure instantanée du champ hydrodynamique sont mis en évidence. Dans un deuxième temps, des zones de mélange diphasiques présentant, soit un gradient de vitesse, soit un gradient de fraction volumique, font l'objet de mesures locales par la même technique. Dans le premier cas, on constate que, par rapport au cas monophasique, la présence de la phase dispersée conduit à une expansion plus rapide de la zone de mélange et à une sensible modification de la turbulence produite par le cisaillement. Dans le deuxième cas, l'écoulement est piloté par les variations à grande échelle de la densité du mélange. Enfin, des comparaisons avec des simulations numériques sont effectuées afin d'évaluer la capacité d'outils numériques actuels à prédire ces différents écoulements liquide-liquide.
Active bandpass filtering with bucket-brigade delay lines (1972)

Bucket-brigade delay lines have been used to build a tunable active bandpass filter. Experimental results showing the dependence of center frequency and bandwidth on the delay-line clock frequency, and the dependence of the center frequency and the Q of the filter on internal gain parameters are presented.
ON THE DEVELOPMENT OF A METHOD TO SYNTHESIZE PROGRAMS FROM REQUIREMENTS SPECIFICATIONS (1991)

This paper reviews the progress that has been made towards the definition of a new generation of computer assisted problem solving tool. When we solve problems with a computer we state the solution in terms of several languages, beginning with a requirements specification language and ending with a program. Beginning with a generalization of a programming language, this paper follows steps towards the removal of control and data structure information in a problem solution. The effort discussed here would result in the requirements specification language being the final language used in problem solving.
Depressed Adenyl Cyclase Activity in the Failing Guinea Pig Heart (1969)

There is considerable evidence that several effects of catecholamines on beta receptors, including the positive inotropic effect on the heart, are mediated by cyclic-AMP. Myocardial cyclic-AMP concentration depends on the activity of at least two enzymes: adenyl cyclase, regulating synthesis, and phosphodiesterase, regulating degradation of the compound. In view of the importance of adrenergic support in maintaining cardiac performance, we examined synthesis and degradation of cyclic-AMP in homogenates of hearts from guinea pigs with congestive heart failure produced by constriction of the ascending aorta and from sham-operated controls. In the experimental group, left ventricular weight, DNA, RNA, and protein content were significantly increased 7 to 12 days following operation, and congestive heart failure developed, manifested by hydrothorax, hepatomegaly, and histologic evidence of pulmonary edema and passive congestion of viscera. Adenyl cyclase activity in homogenates from the experimental group was 36% less than that observed in controls ( P < 0.001). However, phosphodiesterase activity was unchanged. These findings suggest that there is a double biochemical defect in the adrenergic support to the failing heart. In addition to the known reduction of myocardial catecholamines, there appears to be diminished capacity for synthesis of cyclic-AMP, an important mediator of catecholamine action.
Therapeutic Synergy between microRNA and siRNA in Ovarian Cancer Treatment (2013)

This study addresses a new concept of RNA inhibition therapy by combining miRNA and siRNA in nanoliposomal particles to target oncogenic pathways altered in ovarian cancer. Combined targeting of the Eph pathway using EphA2-targeting siRNA and the tumor suppressor miR-520d-3p exhibits remarkable therapeutic synergy and enhanced tumor suppression in vitro and in vivo compared with either monotherapy alone.
Copper-Substituted Zirconium Phosphate—a New Oxidation Catalyst (1974)

A novel catalytically active compound is obtained by substitution of copper cations into crystalline α-zirconium phosphate. Catalytic oxidation of carbon monoxide on crystalline copper-substituted zirconium phosphate was studied at different gas compositions, flow rates, and temperatures up to 400°C. A 1-ft long, tubular reactor with a preheater section is used for analysis. The rate of reaction is proportional to the square root of the carbon monoxide concentration. Oxygen, when present in large excess over its stoichiometric ratio, has no influence on the reaction rate. The apparent activation energy of 12.5 kcal/mole and the rate constant for the reaction are determined. Results indicate that copper-substituted α-zirconium phosphate is comparable in activity with a number of active catalysts for carbon monoxide oxidation.
Update from the''Radiochemistry at RIA'' Symposium held March 27, 2003 at the 225th American Chemical Society Meeting in New Orleans, LA (2003)

The scientific case for a new high-luminosity Radioactive Ion Beam (RIB) Facility capable of accelerating essentially the entire chart of the nuclides has been established over more than a decade.[1-5] Preliminary experiments at new facilities [6] are promising as the frontier of RIB research continues to expand at a fast rate. Radiochemical experiments that could benefit many areas of research, from the basic science of astrophysics and heavy element research, to the applied science of Stockpile Stewardship and medical isotope production, are now for the first time being seriously discussed for a RIB facility. This paper attempts to highlight some areas of research which may be significantly enhanced should a premier radiochemical facility be co-located at a RIB accelerator facility such as the Rare Isotope Accelerator (RIA). The purpose of the symposium was to gather together experts in a variety of fields to discuss the harvesting of radionuclides and its potential benefits to research in the heavy element research, medical isotope production, Stockpile Stewardship Program (SSP), environmental research, medical astrophysics and educational fields. Preliminary ideas as to the engineering features required for such a facility at RIA, to generate, handle, store and use isotopes of interest potentially containing very high levels of radioactivity, were discussed.
A Fringe Carrier Technique For Unambiguous Determination Of Holographically Recorded Displacements (1985)

This paper presents a new technique which eliminates the ambiguity in holographic fringe interpretation. The technique is based upon generating a high density linear phase carrier. Introduction of the carrier results in the formation of a fringe pattern which has mono-tonically increasing fringe orders. Any deformation of the object under study produces a corresponding perturbation of the carrier pattern. If the carrier dominates, the perturbed pattern continues to have monotonically increasing fringe orders and thus allows the fringe order to be determined at any point without ambiguity. The phase change due to deformation alone is obtained by subtracting the known linear carrier from the modulated fringe pattern. Elimination of fringe ambiguity will pave the way for automatic data acquisition in holographic interferometry.
Maximum Likelihood Joint Tracking and Association in a Strong Clutter without Combinatorial Complexity (2010)

We have developed an efficient algorithm for the maximum likelihood joint tracking and association problem in a strong clutter for GMTI data. By using an iterative procedure of the dynamic logic process "from vague-to-crisp," the new tracker overcomes combinatorial complexity of tracking in highly-cluttered scenarios and results in a significant improvement in signal-to-clutter ratio.
Implementation Challenges and Performance of Forced Harmonic Oscillator Model in Direct Simulation Monte Carlo (2013)

The forced harmonic oscillator (FHO) model is an attractive choice for implementation into DSMC due to its higher accuracy over the LarsenBorgnakke (L-B) model, and the fact that it is still an analytic expression. The higher accuracy comes with higher complexity, and several factors which may adversely affect DSMC simulations in particular must be addressed. Detailed balance, round-off errors, and anharmonic oscillator energy limitations, all require some slight modification or cut-off values in the case of the errors in order to ensure its accuracy within DSMC simulations. In addition, quantum jump restrictions to ±5 is shown to reduce the computational time required by as much as 13% while only decreasing accuracy by less than 0.2%. Assessment of the computational resources required for the FHO model in comparison to the commonly used L-B model is performed.
Abstract 11296: Practice Level Variation in Cardiac Performance Measure Compliance: Insights from the American College of Cardiology's Practice Innovation and Clinical Excellence (PINNACLE) Program (2010)

Background: While the American College of Cardiology's Practice Innovation And Clinical Excellence (PINNACLE) Program has reported rates of compliance for performance measures for coronary artery d...
An Improved Procedure for the Preparation of β-Nitroethylamines (1991)

nitroethilene and nitro styrene generated in situ from 2.benzoyloxy-1-nitroetthane and 2-benzoyloxy-1-nitro-1-phenylethane respectively reacted with aliphatic amines to give the corresponding Michael adducts in near quantitative yield
The time-local Fokker–Planck equation (1990)

A time-local Fokker–Planck equation (TLFPE) is derived which accounts for memory effects in stochastic problems. This is expected to provide a computationally efficient method of modeling the phase space evolution of such systems by simple (local time) Langevin equations with Markovian fluctuating forces that are characterized by time-dependent moments; it is this explicit time dependence that describes the memory effects. The TLFPE is derived from the probability theory of non-Markovian systems as a generalization of Chandrasekar’s derivation of the Fokker–Planck equation (FPE) from the Chapman–Kolmogarov equation for Markovian systems. In this article it is applied to free particle diffusion and barrier crossing problems, and is shown to give rise to physically realistic results. Further, the form of the TLFPE suggests that the conditions required for systems to exhibit Markovian behavior are less restrictive than the Brownian criterion of separation of time scales between the fluctuating forces and the momentum response of the system. Rather, a sufficient condition is that the time-dependent moments of the TLFPE reach plateau values before the time scale of the phenomenon of interest.
On the role of intermolecular hydrogen bonding in miscible polymer blends (1984)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTOn the role of intermolecular hydrogen bonding in miscible polymer blendsE. J. Moskala, S. E. Howe, Paul C. Painter, and Michael M. ColemanCite this: Macromolecules 1984, 17, 9, 1671–1678Publication Date (Print):September 1, 1984Publication History Published online1 May 2002Published inissue 1 September 1984https://pubs.acs.org/doi/10.1021/ma00139a006https://doi.org/10.1021/ma00139a006research-articleACS PublicationsRequest reuse permissionsArticle Views960Altmetric-Citations180LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
A synergistic interaction between lapatinib and topoisomerase I inhibitors in cisplatin sensitive and resistant cancer cell lines (2008)

14618 Background: Lapatinib is a HER1 and 2 tyrosine kinase inhibitor (TKI). Little is know about its interaction with other chemotherpy drugs. In this work investigate interactions between lapatinib and cisplatin or SN-38 (the active metabolite of irinotecan) in cisplatin sensitive (testis cancer) and resistant cancer cell lines. Methods: We obtained 3 cancer cells lines (833K, GCT27, SUSA) all of which had cisplatin resistant daughter lines (833Kr, GCT27r, SUSAr). These resistant lines were established by exposing the sensitive lines to increasing doses of cisplatin. Western Blot analysis was used to estimate HER 1–4 levels and expression of proteins in the PI3K and MAPK pathways. The IC 50s for lapatinib, cisplatin and SN-38 were calculated in these cell lines. These drugs were then given in combination, and the effect was estimated using calcusin plots. Flow cytomery was used to estimate the effect on the cell cycle and apoptosis. Identical experiments were carried out with Pazopanib, instead of lapatinib, which was used as a control. Results: The IC50 for lapatinib in the resistant and sensitive cell lines were similar (4.0–5.0 micromolar and 3.6–4.4 micromolar respectively). Sensitivity was not related to HER 1–4 expression. Synergistic interactions were observed between SN-38 and lapatinib in all 6 cell lines (calculated combination indices <0.7 in all lines). No synergistic interaction was seen between cisplatin and lapatinib. Cell cycle data revealed the synergistic combination was associated with an increase in apoptosis and reduces cell cycle arrest. Pazopanib was not associated with a synergistic interaction in any of the cell lines with either cisplatin or SN-38. Conclusions: Lapatinib appears equally efficacous in both cisplatin sensitive and resistant cell lines. A synergistic interaction occurs between lapatinib and SN-38 in this model which is associated with increased apoptosis. Author Disclosure Employment or Leadership Consultant or Advisory Role Stock Ownership Honoraria Research Expert Testimony Other Remuneration GlaxoSmithKline
ChemInform Abstract: Synthesis of C‐Oligosaccharides (2001)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.