gebhart/patrepeval huggingface dataset:
Molecular and Functional Characterization of Caveolae in Mixed Cultures of Human NT-2 Neurons and Astrocytes (2021)

Caveolae are plasma membrane invaginations that are enriched in cholesterol-binding proteins called caveolins. The presence of caveolae and caveolins in mixed cultures of human neurons and glia has not been investigated. Here, we sought to determine the presence of caveolae and caveolins in human NTera-2 (NT2/D1) cells, differentiated with retinoic acid into neuron-like (NT2/N) and astrocyte-like (NT2/A) cells. We found that while caveolin-3 mRNA levels remained relatively constant, caveolin-1 and -2 levels were upregulated in NT2/A and downregulated in NT2/N. No caveolin-1 immunoreactivity was detected in NT2/N. Electron microscopy revealed numerous flask-shaped invaginations (~86–102 nm in diameter) in the plasma membrane of NT2/A and NT2/N cells, while only few were detected in NT2/D1 cells. Immunoelectron microscopy localized caveolin-1 gold particles in the flask-shaped structures on plasmalemma and cytoplasmic vesicles of NT2/A cells. Furthermore, NT2/A endocytosed Alexa 488 conjugated-cholera toxin B subunit (CTX-B) through a caveolae- and clathrin-dependent pathway, whereas NT2/N endocytosed CTX-B through a caveolae-independent pathway. We have established that while NT2/A expressed functional caveolae, the molecular identity of the plasma membrane invaginations in NT2/N is unknown. The expression of caveolin proteins was differentially regulated in these cells. Taken together, our findings support the usefulness of the human NT2 model system to study the role of caveolins in neuron–glia communication, and their involvement in brain health and disease.
Diazoxide induces delayed preconditioning against transient focal cerebral ischemia in Rats (2006)

Diazoxide is the prototypical opener of mitochondrial ATP‐sensitive potassium channels (mitoK ATP ) and protects neurons in vivo and in vitro against chemical and anoxic stresses. While we have previously shown that diazoxide administration induces delayed protection of the blood‐brain barrier against ischemic damage in rats ( Brain Res. 2005; 1051 :–80), we did not examine whether diazoxide limits infarct volume in stroke. The purpose of this study was to determine whether diazoxide promotes delayed preconditioning against experimental strokes. We tested the delayed effect of Diazoxide on 90 min middle cerebral artery occlusion (MCAO) in male Wistar rats. Diazoxide (10mg/kg) was injected 24 hours before MCAO intraperitonealy. Infarct volumes were measured 72 hr after reperfusion. Treatment with diazoxide 24 hr before the onset of ischemia exhibited a 28 % reduction (235.2±7.1 vs. 169.2± 22.5mm 3 ) and a 32 % reduction (179.6±2.0 vs.122.0±18.0mm 3 ) in infarct volumes for total brain and cortex, respectively. These findings are consistent with the assumption that delayed preconditioning is produced by the activation of mitoK ATP in the rat brain. Supported by NIH grants (HL‐30260, HL‐46558, HL‐50587, DK‐62372) for D. W. Busija.
Assembly of generators with rated voltage higher than 100 kV (2002)

Powerformer/sup TM/ is a newly developed synchronous machine that generates electric power directly at transmission voltage levels. This is achieved by replacing the rectangular conductors in the traditional stator winding with circular conductors based on proven high voltage cable technology with solid extruded dielectric. The new machine is designed for both hydro and thermal applications. The concept with cables, as stator winding, requires new techniques in the design and assembly process. This paper discusses the concept of Powerformer and the assembling process that has been developed for the following two cases: Eskilstuna rated 42 MVA at 136 kV, which will be used in a thermal application and was assembled in the workshop, and Porsi rated 75 MVA at 155 kV for a hydropower application and assembled at site. Finally, some future outlooks regarding applications for Powerformer are presented.
Fluidized bed drying of a pharmaceutical powder: A parametric investigation of drying of dibasic calcium phosphate (2016)

A parametric analysis of four fluidized bed dryer operating parameters—initial moisture content, material loading, heating air temperature, and air flux—was carried out using two factorial experimental designs. Two different dryer scales, the Glatt GPCG-1 and MiniGlatt, with chamber volumes of 23 and 3 L, respectively, were used to dry dibasic calcium phosphate powders. According to a Pareto analysis, initial moisture content and air flux were the most significant variables. For the larger GPCG-1, the four variables used in the factorial tests were found to be independent. For the smaller MiniGlatt, the interaction of the initial moisture content and the air flow rate was also significant. From the parametric analysis, it was observed that drying is dominated by the constant drying stage, and zero-order kinetics, for the mild drying conditions (appropriate for many pharmaceutical products) we considered in this work. A comparison of the two dryers and an evaluation of energy efficiency were also carried out. It was found that drying should be carried out with relatively low temperatures and high air fluxes for both the large- and small-scale dryers in order to maximize the energy efficiency.
Electrocyclic reactions of open metallocenes: carbon-carbon bond formation during thermolysis of bis(2,4-dimethyl-1,3-pentadienyl)ruthenium and -osmium (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTElectrocyclic reactions of open metallocenes: carbon-carbon bond formation during thermolysis of bis(2,4-dimethyl-1,3-pentadienyl)ruthenium and -osmiumRein U. KirssCite this: Organometallics 1992, 11, 2, 497–499Publication Date (Print):February 1, 1992Publication History Published online1 May 2002Published inissue 1 February 1992https://pubs.acs.org/doi/10.1021/om00038a001https://doi.org/10.1021/om00038a001research-articleACS PublicationsRequest reuse permissionsArticle Views108Altmetric-Citations15LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Asymptotically optimal dynamic pricing for network revenue management (2012)

A dynamic pricing problem that arises in a revenue management context is considered, involving several resources and several demand classes, each of which uses a particular subset of the resources. The arrival rates of demand are determined by prices, which can be dynamically controlled. When a demand arrives, it pays the posted price for its class and consumes a quantity of each resource commensurate with its class. The time horizon is finite: at time $T$ the demands cease, and a terminal reward (possibly negative) is received that depends on the unsold capacity of each resource. The problem is to choose a dynamic pricing policy to maximize the expected total reward. When viewed in diffusion scale, the problem gives rise to a diffusion control problem whose solution is a Brownian bridge on the time interval $[0,T]$. We prove diffusion-scale asymptotic optimality of a dynamic pricing policy that mimics the behavior of the Brownian bridge.\n¶\nThe ‘target point’ of the Brownian bridge is obtained as the solution of a finite dimensional optimization problem whose structure depends on the terminal reward. We show that, in an airline revenue management problem with no-shows and overbooking, under a realistic assumption on the resource usage of the classes, this finite dimensional optimization problem reduces to a set of newsvendor problems, one for each resource.
Taking the wheel (2006)

Subtitle: New FMCSA chief John Hill says safety is top priority, seeks to slice rules backlog.
Long-Term Clinical Outcome After Postchemotherapy Retroperitoneal Lymph Node Dissection in Men With Residual Teratoma (2007)

PC-RPLND remains critical in the management of patients with NSGCT. Patients found to have teratoma at PC-RPLND have a 10-year probability of freedom from recurrence of 80%. The size of the residual mass and IGCCCG risk classification were significant predictors of disease recurrence.
Approximation of the Two-Part MDL Code (2006)

Approximation of the optimal two-part MDL code for given data, through successive monotonically length-decreasing two-part MDL codes, has the following properties: (i) computation of each step may take arbitrarily long; (ii) we may not know when we reach the optimum, or whether we will reach the optimum at all; (iii) the sequence of models generated may not monotonically improve the goodness of fit; but (iv) the model associated with the optimum has (almost) the best goodness of fit. To express the practically interesting goodness of fit of individual models for individual data sets we have to rely on Kolmogorov complexity.
4,6-Di-O-pentyl-3-O-trifluoroacetyl/propionyl cyclofructan stationary phases for gas chromatographic enantiomeric separations (2011)

4,6-Di-O-pentyl-3-O-trifluoroacetyl cycloinulohexaose (DP-TA-CF6) and 4,6-di-O-pentyl-3-O-propionyl cycloinulohexaose (DP-PN-CF6) were synthesized and used as chiral stationary phases (CSPs) in gas chromatography (GC). The chiral recognition ability of the two CSPs was investigated. A total of 47 racemic compounds were separated on the two new CSPs, including derivatized amino acids, amino alcohols, amines, alcohols, tartrates and lactones. Interestingly, several analytes were only separated on either the DP-TA-CF6 or the DP-PN-CF6 phase. The chiral recognition mechanism was evaluated through thermodynamic analysis. The result indicated there was no inclusion complex formation involved in the chiral recognition process.
Effect of GAC characteristics on adsorption of organic pollutants (1993)

ABSTRACT: The impact of the characteristics of granular activated carbon (GAC) on adsorption capacity and on the potential for polymerization of phenolic compounds on the surface of GAC in the presence of molecular oxygen is evaluated in this study. Adsorption isotherm data were collected for p ‐chlorophenol on five activated carbons; three manufactured from bituminous coal, one manufactured from lignite coal, and one manufactured from wood. These isotherms were collected under anoxic (absence of molecular oxygen) and oxic (presence of molecular oxygen) conditions. Higher extraction efficiencies of the carbons used in the oxic isotherms were obtained for the carbons that exhibited lower increases in capacities when compared to anoxic isotherms. Furthermore, no impact of the presence of oxygen on adsorption capacity was noted for the wood base carbon. Breakthrough curves developed for p ‐chlorophenol on the five carbons studied have shown tailing effects for the carbons that demonstrated differences in capacities under oxic and anoxic conditions whereas no tailing was noticed for the wood base carbon.
Further Characterization of the Guinea Pig Cerebral Cortex Idazoxan Receptor: Solubilization, Distinction from the Imidazole Site, and Demonstration of Cirazoline as an Idazoxan Receptor‐Selective Drug (1990)

We have demonstrated previously that [3H]idazoxan, besides being able to bind to alpha 2-adrenergic receptors, may also bind to a nonadrenergic idazoxan-receptor site with high affinity. The idazoxan receptor is tightly bound to cellular membranes, and we have now developed a method to solubilize it from the guinea pig cerebral cortex by using the detergent 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS). The CHAPS-solubilized receptor retains its binding properties for drugs: the membrane-bound, as well as the solubilized, idazoxan receptor shows high affinities for a number of imidazolines (cirazoline, idazoxan, tolazoline, naphazoline, tramazoline, clonidine, and oxymetazoline), some imidazoles (medetomidine, detomidine), and guanfacine. By contrast, catecholamines (adrenaline, noradrenaline, isoprenaline, and dopamine) and a number of other neurotransmitters and neuromodulators (serotonin, histamine, glutamic acid, gamma-aminobutyric acid, glycine, and adenosine) show negligible affinities for the idazoxan receptor. Moreover, the idazoxan receptor shows grossly different binding properties for histamine, cimetidine, and imidazole-4-acetic acid compared to what has been described for the nonadrenergic imidazole site labeled by p-[3H]amino-clonidine, indicating that the two receptor sites are distinct. Radioligand binding data further indicate that cirazoline is an idazoxan receptor-selective drug (KD = 1 nM) showing a 50-210-fold selectivity for binding to the idazoxan receptor when compared to alpha 2-adrenergic receptors and an about 500-fold selectivity when compared to alpha 1-adrenergic receptors. We have also reviewed the literature for possible nonadrenergic actions of idazoxan and cirazoline, and we suggest that idazoxan receptors might be involved in the control of prolactin release from the pituitary.
THE STRUCTURE AND THE MEDIUM RANGE ORDER IN THIN AMORPHOUS GERMANIUM FILMS PREPARED IN UHV (1982)

Stable amorphous germanium (a-Ge) films were prepared by slow evaporation onto heated sapphire substrates in ultra high vaccuum (UHV). The structure of these films was investigated by in-situ transmission high energy electron elastic diffraction performed at 77K, by in-situ density measurements and by in-situ determination of the low energy refractive index. The density and the refractive index measurements indicated that thin, stable a-Ge films are appreciably less dense than the crystalline counterpart. The low temperature electron diffraction experiments showed some changes in the positions of the reciprocal space interference maxima, when compared with work of others. Also, for the first time, a "sharp" diffraction pre-peak (at s = 1.2 Å-1) has been observed, a diffraction feature generally seen in disordered systems. The results are discussed in terms of the coordination and the range of the electronic bonding interactions in glass.
Calcium Antagonists and Islet Function. II. Interaction of Theophylline and Verapamil (1976)

Verapamil inhibits glucose-induced insulin release by the isolated perfused rat pancreas. The dose-response relationship for the inhibitory action of verapamil is shifted to higher concentrations of the drug when the concentration of calcium in the perfusate is increased. The degree of inhibition of glucose-induced insulin release by a given concentration of verapamil decreases as the length of exposure to glucose is increased prior to introduction of the calcium antagonist. Theophylline augments glucose-stimulated insulin release and protects the beta-cell against the inhibitory action of verapamil, both effects of theophylline being dose-related. Even after pretreatment of the pancreas with a high concentration of verapamil, theophylline is able, in the presence of glucose, to stimulate insulin release and, eventually, to restore a nearly normal secretory response to this sugar. We suggest that theophylline, by mobilizing calcium from an organelle-bound pool within the beta-cell, compensates for the verapamil-induced reduction in calcium inward transport into the beta-cell.
Synthesis of <sup>14</sup>C‐labeled 5‐[1‐hydroxy‐2‐[2‐(<i>o</i>‐methoxyphenoxy) ethylamino] ethyl]‐2‐methylbenzenesulfonamide hydrochloride (YM‐09538) (1983)

Abstract Preparation of the title compound, a novel antihypertensive agent, is described. The labeled material with a specific activity of 4.76 mCi/mmol was synthesized in a 50.6% radiochemical yield from [U‐ 14 C]ethylene oxide via a process of six steps.
Description of the global energy systems model GET-RC 6.1 (2013)

A series of stimuli-responsive fluorescent hydrogels were successfully synthesized via micelle radical copolymerization of hydrophilic acrylamide (AM), hydrophobic chromophore terpyridine-based monomer (TPY), and <i>N</i>-isopropylacrylamide (NIPAM). These hydrogels presented blue emissions (423-440 nm) under room temperature, which is caused by the π-π* transition of the conjugated structures. Once the ambient temperature was increased to 55 °C, the fluorescence color changed from blue (430 nm) to pink (575 nm) within 10 min, subsequently to yellow (535 nm), and eventually back to pink. The thermal-responsive properties are attributed to the transition of the TPY units from unimer to dimer aggregation via the intermolecular charge transfer complex at high temperatures. The hydrogels showed pH-responsive properties. The emission peak of the hydrogel exhibited a blue shift of ~54 nm from neuter conditions to acidic conditions, while a 6 nm red shift to an alkaline environment was observed. The hydrogels demonstrated an obvious change in fluorescence intensity and wavelength upon adding different metal ions, which is caused by the coordination between the terpyridine units incorporated on the backbones and the metal ions. As a consequence, the hydrogels presented a sharp quenching fluorescence interaction with Fe<sup>2+</sup>, Fe<sup>3+</sup>, Cu<sup>2+</sup>, Hg<sup>2+</sup>, Ni<sup>2+</sup>, and Co<sup>2+</sup>, while it exhibited an enhanced fluorescence intensity interaction with Sn<sup>2+</sup>, Cd<sup>2+</sup>, and Zn<sup>2+</sup>. The microstructural, mechanical, and rheological properties of these luminescent hydrogels have been systematically investigated.
Dementia in Parkinson disease (1979)

In 520 patients with parkinsonism seen over eight years, 168 (32%) had moderate to marked dementia. Although the demented patients were older than the nondemented patients (70.4 versus 65.5 years), the incidence of dementia in Parkinson's disease (PD) was tenfold higher than among controls (similarly aged spouses of PD patients), and dementia is held to be related more to the disease than to age. Demented patients, in addition to being older, developed PD later, were more severely involved in a shorter time, and responded less well to levodopa. It is suggested that PD with dementia may represent a different disorder from PD without dementia.
Implementations of the LMT heuristic for minimum weight triangulation (1998)

No polynomial-time algorithm is kno-ivn to compute t,he minimum weight triangulation (MWT) of a finite planar point set. In this paper xve present efficient implementat,ions of the LMT-skeleton heurist'ic, xvhich identifies edges that must be, and cannot be, in an MWT. For uniformly distributed points, v:e can compute the esact MWT of tens of thousands of points in minutes.
Determination of metal ions in single wood fiber by LA-ICP-MS (2012)

Abstract Laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) was used to determine the distribution of metal elements in single wood fiber of kraft pulp. Calibration procedures for quantitative analysis of metals in single fibers (SFs) by LA-ICP-MS technique were also developed. Calibration curves were constructed by using matrix-matched standards prepared from (a) cellulose powder washed with 0.05 M EDTA, (b) unbleached softwood pulp washed with 0.05 M EDTA and nitric acid. Calibration materials were prepared by doping the washed cellulose powder and the washed unbleached softwood pulp with standard solutions with increasing concentrations of the elements of interest (Co, Mg, Cu, Na, K, Mn, Zn, Ni, Ca, and Fe). Quantitative determination of the metals in single wood fiber was based on the calibration curves resulting from both the doped cellulose pellets and the doped SFs. The determination of the absolute concentration of elements in a single wood fiber by LA-ICP-MS is still difficult, but in this study, it was demonstrated that the information about the metal distribution can be obtained easily, and their semiquantitative determination is possible.
Puma Is The Critical BH3-Only Protein Mediating Apoptosis In The Nup98-HoxD13 (NHD13) Mouse Model Of Human MDS (2013)

Abstract One of the key hallmarks of myelodysplastic syndromes (MDS) is that of ineffective haematopoiesis. The main factor contributing to this is increased apoptosis of bone marrow progenitors – a prominent feature particularly of early-stage MDS. Although apoptosis has been attributed to cell extrinsic triggers such as increased levels of Fas and TNF-α, there is increasing evidence implicating the cell intrinsic pathway – in which Bcl-2 and its related proteins play an important role and which is activated by p53. Consistent with this, we have recently shown that apoptosis in the Nup98-HoxD13 (NHD13) transgenic mouse model of MDS can be effectively blocked by enforced expression of Bcl-2. To further define the molecular mechanism of apoptosis upstream of Bcl-2, we mated NHD13 mice with mice deficient for p53. Consistent with a lineage specific role for p53, we observed p53-dependent apoptosis of the erythroid, but not the myeloid lineage. In NHD13 progenitors, gene expression analysis for members of the intrinsic pathway, showed elevated expression of Noxa but not Puma – both of which are BH3-only proteins and transcriptional targets of p53. We proceeded to mate NHD13 mice with mice deficient in Noxa and Puma. Unexpectedly, the expression levels were in fact misleading as genetic ablation studies showed that apoptosis of both erythroid and myeloid lineages was Puma dependent, with no role for Noxa. These studies have demonstrated that gene expression of Bcl-2 proteins cannot be used to predict functional relevance and in this case, Puma is the critical BH3-only protein mediating apoptosis in MDS. Disclosures: Guirguis: Leukaemia Foundation - Australia: Research Funding.