MP20-08 PYURIA AND ASYMPTOMATIC BACTERIURIA IS ASSOCIATED WITH NOVEL AND SPECIFIC URINE MICROBIOMES (2015)

You have accessJournal of UrologyInfections/Inflammation of the Genitourinary Tract: Kidney & Bladder II1 Apr 2015MP20-08 PYURIA AND ASYMPTOMATIC BACTERIURIA IS ASSOCIATED WITH NOVEL AND SPECIFIC URINE MICROBIOMES Suzanne Groah, Marcos Perez-Losada, Ljubica Caldovic, Inger Ljungberg, Bruce Sprague, Eduardo Castro-Nallar, Neel Shah, Michael Hsieh, and Hans Pohl Suzanne GroahSuzanne Groah More articles by this author , Marcos Perez-LosadaMarcos Perez-Losada More articles by this author , Ljubica CaldovicLjubica Caldovic More articles by this author , Inger LjungbergInger Ljungberg More articles by this author , Bruce SpragueBruce Sprague More articles by this author , Eduardo Castro-NallarEduardo Castro-Nallar More articles by this author , Neel ShahNeel Shah More articles by this author , Michael HsiehMichael Hsieh More articles by this author , and Hans PohlHans Pohl More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2015.02.980AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Pyuria in the setting of asymptomatic bacteriuria (ABU), particularly in the context of potentially unrelated lower and upper urinary tract symptoms, may drive inappropriate use of antibiotics. A deeper comprehension of the relationship between pyuria and ABU will be essential in improving antibiotic use, especially in patients with neuropathic bladder, a disorder which features increased risk of ABU and UTI. METHODS This is a cross-sectional study of asymptomatic subjects with normally functioning bladders (non-NB) and with neuropathic bladder (NB). Urine was obtained using the subject's typical method of urine elimination (mid stream spontaneous void, sterile intermittent catheterization or aspiration from suprapubic tube). Samples were analyzed using 16S rRNA pyrosequencing in combination with Pathoscope 2.0, STAMP, urinalysis, and urine culture. RESULTS All patients, regardless of bladder status and gender had bacteriuria based on 16s rRNA pyrosequencing with only 20/47 having positive urine cultures. Subjects with NB had significantly greater positive leukocyte esterase, pyuria, and cloudy urine on urinalysis, as well as bacterial growth on urine culture. Unique urine microbiomes were characterized for all subjects and grouped according to subject characteristics. Actinobaculum was found in significantly higher (p=0.009) proportions of patients with pyuria. Whereas Lactobacillus was more abundant in patients without pyuria. CONCLUSIONS Our data indicate that specific microbiomes are linked to pyuria-associated ABU. The discovery of a correlation between pyuria, ABU, and Actinobaculum, an emerging and poorly understood uropathogen, highlights the potential of microbial genomics to identify highly fastidious and non-culturable bacteria which may contribute to and drive lower urinary tract symptoms. © 2015 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 193Issue 4SApril 2015Page: e226 Advertisement Copyright & Permissions© 2015 by American Urological Association Education and Research, Inc.MetricsAuthor Information Suzanne Groah More articles by this author Marcos Perez-Losada More articles by this author Ljubica Caldovic More articles by this author Inger Ljungberg More articles by this author Bruce Sprague More articles by this author Eduardo Castro-Nallar More articles by this author Neel Shah More articles by this author Michael Hsieh More articles by this author Hans Pohl More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...