gebhart/patrepeval huggingface dataset:
Development of monoclonal antibodies specific for the calpain‐generated ∆48 kDa calcineurin fragment, a marker of distressed astrocytes (2020)

Abstract Background Calcineurin (CN) is a Ca2+/calmodulin‐dependent protein phosphatase expressed at high levels in brain. In healthy tissue, CN exists mainly as a full‐length (∼60 kDa) highly‐regulated protein involved in essential cellular functions. However, in diseased or injured tissue, CN is proteolytically converted to a constitutively active fragment that has been causatively‐linked to numerous pathophysiologic processes. The 48 kDa CN fragment (DCN) appears at high levels in human brain at early stages of cognitive decline associated with Alzheimer’s disease. DCN tends to show‐up in regions of frank amyloid and cerebrovascular pathology, especially in select subsets of astrocytes, both in humans and in animal models. Our goal was to develop monoclonal antibodies to DCN for use in neuropathology research. Method A peptide encompassing the calpain sensitive region of the CN carboxyl terminus was used for antibody generation. Antibodies were screened in ELISAs against the immunizing peptide, but decision‐making screens were carried out as a Western analysis of 5XFAD mouse brain extracts, which express high levels of the DCN fragment. Result We identified 2 monoclonals, one of which (17E1) was highly reactive to DCN in Western blots, but not in ELISAs, of 5xFAD brain extracts. In contrast, the second antibody (26A6) reacted well against the peptide in ELISAs, but worked only modestly in Westerns. Importantly, neither antibody reacted with full‐length (60 kDa) CN, nor did antibodies detect DCN in extracts from healthy WT mice. In immunostaining assays, both antibodies strongly labeled subsets of GFAP‐positive astrocytes in 5xFAD mice with a high signal‐to‐background. Some GFAP‐negative cells also labeled positively for DCN. Consistent with Westerns, there was no antibody reactivity to WT mouse brain sections. Conclusion New monoclonals are highly selective for the 48 kDa CN proteolytic fragment and label subsets of astrocytes, and possibly other cell types, under pathological conditions. These antibodies could be a useful tool for marking insidious brain pathology and identifying novel astrocyte phenotypes.
Population Aging and Economic Progress in Asia: A Bumpy Road Ahead? (2011)

For more about the East-West Center, see <a href="http://www.eastwestcenter.org/">http://www.eastwestcenter.org/</a>
MAPPIT: a versatile tool to study cytokine receptor signalling (2008)

MAPPIT (mammalian protein-protein interaction trap) is a cytokine receptor-based two-hybrid method that operates in intact mammalian cells. A bait is fused C-terminally to a STAT (signal transducer and activator of transcription) recruitment-deficient receptor, whereas the prey is linked to functional STAT-binding sites. When bait and prey interact a ligand-dependent complementation of the STAT recruitment deficiency occurs, leading to activation of a STAT-responsive reporter. MAPPIT is very well suited to study protein interactions involving activated cytokine receptors as the technique allows modification of the bait protein in a physiologically optimal environment.
Methylation of nucleosides with trimethylsulfonium hydroxide. Effects of transition metal ions (1980)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMethylation of nucleosides with trimethylsulfonium hydroxide. Effects of transition metal ionsKiyoshi Yamauchi, Toru Nakagima, and Masayoshi KinoshitaCite this: J. Org. Chem. 1980, 45, 19, 3865–3868Publication Date (Print):September 1, 1980Publication History Published online1 May 2002Published inissue 1 September 1980https://pubs.acs.org/doi/10.1021/jo01307a026https://doi.org/10.1021/jo01307a026research-articleACS PublicationsRequest reuse permissionsArticle Views443Altmetric-Citations17LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Formation of F-ring Isoprostane-like Compounds (F3-Isoprostanes) in Vivo from Eicosapentaenoic Acid (2006)

Eicosapentaenoic acid (EPA, C20:5, omega-3) is the most abundant polyunsaturated fatty acid (PUFA) in fish oil. Recent studies suggest that the beneficial effects of fish oil are due, in part, to the generation of various free radical-generated non-enzymatic bioactive oxidation products from omega-3 PUFAs, although the specific molecular species responsible for these effects have not been identified. Our research group has previously reported that pro-inflammatory prostaglandin F2-like compounds, termed F2-isoprostanes (IsoPs), are produced in vivo by the free radical-catalyzed peroxidation of arachidonic acid and represent one of the major products resulting from the oxidation of this PUFA. Based on these observations, we questioned whether F2-IsoP-like compounds (F3-IsoPs) are formed from the oxidation of EPA in vivo. Oxidation of EPA in vitro yielded a series of compounds that were structurally established to be F3-IsoPs using a number of chemical and mass spectrometric approaches. The amounts formed were extremely large (up to 8.7 + 1.0 microg/mg EPA) and greater than levels of F2-IsoPs generated from arachidonic acid. We then examined the formation of F3-IsoPs in vivo in mice. Levels of F3-IsoPs in tissues such as heart are virtually undetectable at baseline, but supplementation of animals with EPA markedly increases quantities up to 27.4 + 5.6 ng/g of heart. Interestingly, EPA supplementation also markedly reduced levels of pro-inflammatory arachidonate-derived F2-IsoPs by up to 64% (p < 0.05). Our studies provide the first evidence that identify F3-IsoPs as novel oxidation products of EPA that are generated in vivo. Further understanding of the biological consequences of F3-IsoP formation may provide valuable insights into the cardioprotective mechanism of EPA.
Focused nonlinear phase-conjugate waves generated by a solid parametric amplifier (2005)

An experimental and numerical study is reported on focused, phase-conjugated sound beams of finite amplitude produced in water by a solid-state parametric amplifier, referred to as the conjugator. The sound beams incident on the conjugator were radiated by a focused circular source. Field distributions were measured along the beam axis and in the focal plane of the source. Both linear and nonlinear propagation were investigated. Apertures of different diameters were placed in front of the conjugator to demonstrate diffraction effects associated with size of the active surface of the conjugator. Peak-to-peak acoustic pressures of 8MPa and strongly distorted, asymmetric waveforms were measured at the focus of conjugate beams having a fundamental frequency of 5MHz. Numerical simulations of harmonic generation in the conjugate beams are in agreement with the measurements. The study reveals that the conjugate beams accurately reproduce the focal region of the incident beams when a sufficiently large aperture is used, even when substantial waveform distortion due to nonlinear propagation effects are observed in the conjugate beams.
Patients' perceptions of a telemedicine specialty clinic (2000)

We studied patients' perceptions of telemedicine (interactive video) as a means of delivering specialist oncology/haematology care. Semistructured telephone interviews were performed with 22 patients attending a tele-oncology/haematology clinic in Kansas. Interviews were audio-taped and thematic content analysis of the transcripts was done. The response rate was 96%. All participants expressed satisfaction with their tele-consultations. For 50% of respondents, satisfaction with the tele-oncology/haematology clinic was qualified by two factors: participants also saw the specialist in person on occasions and the clinic was perceived as providing mainly a 'monitoring' function. In addition, 9 of the 22 patients expressed concern about the role of the nurse as a proxy for the doctor in performing certain parts of the physical examination. Overall, participants valued improved access to specialist services but had clear views as to the limitations of such a service. As the use of telecommunications technology grows, patient perspectives merit greater attention.
Review Essay (2006)

Cultural Intelligence: Individual Interactions across Cultures. P. Christopher Earley and Soon Ang. Palo Alto: Stanford University Press, 2003. CQ: Developing Cultural Intelligence at Work. P. Christopher Earley, Soon Ang and Joo-Seng Tan. Palo Alto: Stanford University Press, 2006.
Fusion Proteins of Single-Chain Variable Fragments Derived from Phage Display Libraries Are Effective Reagents for Routine Diagnosis of Potato Leafroll Virus Infection in Potato (1999)

ABSTRACT A panel of 11 different single-chain variable fragment antibodies (scFv) that bind to potato leafroll virus (PLRV) has been studied to assess each one's suitability as practical diagnostic tools. The scFv, previously obtained from naive phage display libraries, were expressed in Escherichia coli as fusion proteins. The fusion proteins comprised scFv joined to either the human light chain kappa constant domain (C(L)), an amphipathic helix (Zip), a combination of C(L) and Zip, or alkaline phosphatase (AP/S). The fusion proteins were tested for their ability to detect, or trap on enzymelinked immunosorbent assay (ELISA) plates, PLRV in extracts of infected potato leaves. The tests done with the different scFv fusion proteins were compared with a standard triple-antibody sandwich (TAS)-ELISA that employs a rabbit polyclonal antibody preparation to coat microtiter plates and a monoclonal antibody, SCR3, to detect PLRV. Of 11 scFvC(L) fusion proteins, 7 detected PLRV as readily as SCR3 when used as detecting antibodies in TAS-ELISA. The limit of detection of purified PLRV for the different scFvC(L) fusion proteins ranged from 250 to 5 ng/ml; that for SCR3 is 5 ng/ml. Of the 11 scFv, 4 cross-reacted with some other luteoviruses. Several scFvC(L) and scFvC(L)Zip fusion proteins trapped PLRV from extracts of infected potato leaves as effectively as the polyclonal antibody preparation. Four scFv fusion proteins were used in a stem print assay to detect PLRV, and the results were similar to those obtained in tests using SCR3. The scFvC(L) fusion proteins retained activity for at least 6 months at 4 degrees C, and all scFv fusion proteins were fully active on reconstitution after lyophilization. A fully recombinant ELISA was devised that detected PLRV in extracts of infected potato, with results comparable to those obtained using the standard TAS-ELISA. The advantages of using scFv fusion proteins for the routine detection of plant viruses include the ability to produce large quantities of reagents cheaply in bacterial fermenters and to incorporate them into standardized tests.
Open Framework Middleware for intelligent WSN topology adaption in smart buildings (2009)

Modern smart buildings utilize sensor networks for facilities management applications such as energy monitoring. However as buildings become progressively more embedded with sensor networks, the challenge of managing and maintaining the sensor networks themselves becomes ever more significant. As a cost-sensitive activity, facilities management deployments are less likely to deploy node redundancy and specialized technical staff to maintain these networks. Hence there are strong requirements for the network to efficiently self-diagnose, self-heal and integrate with standard buildings management systems. This paper introduces a solution for WSN management in smart buildings that addresses these issues. It is based on the deployment of the open framework middleware for sensor networks coupled with a structured knowledge and rule-based fault analysis engine to perform network event correlation and root cause analysis. The system also explicitly interfaces with a building management system (BMS) or the scheduling of network maintenance activities such as sensor battery replacement.
Large-Area Oblique-Aligned ZnO Nanowires through a Continuously Bent Columnar Buffer: Growth, Microstructure, and Antireflection (2010)

We demonstrate a novel defect-induced bending mechanism for a modified oblique-angle deposition (OAD) system, where different defect density was introduced to accommodate the mass difference between the shadowed and exposed surfaces, leading to continuous structural bending. Oblique angle sputtering and hydrothermal processes were employed for growth of inclined ZnO nanowire arrays on ZnO bent columns. Transmission electron microscopy images reveal that a dislocation network was introduced to accommodate the mass difference in bent columns, and the bending angle could be controlled by growth temperature. Nanowires were then grown along the tangent lines of the bent column tips. The bent column curvature and limited space determine the nanowire growth direction. The reflectance measurements demonstrate that the oblique-aligned ZnO nanowire arrays are an excellent candidate for antireflection coatings, showing the significant suppression of reflectance of 87.5% and 90.0% for polished Si under TE and TM polarization, respectively. The interference oscillations of reflectance show the optical anisotropy of oblique-aligned ZnO nanowire arrays, which is dependent on the angle range of nanowire direction.
Common Genetic Variation in Five Thrombosis Genes and Relations to Plasma Hemostatic Protein Level and Cardiovascular Disease Risk (2006)

A comprehensive survey of common sequence variation demonstrates that cis-regulatory SNPs explain a modest proportion of the residual variance in circulating fibrinogen and factor VII level and PLAT haplotypes increase the risk of CVD. Additional studies are warranted to confirm the association of PLAT sequence variation and risk of CVD.
Natural convection in a horizontal layer of fluid with a periodic array of square cylinders in the interior (2004)

This study of thermal convection uses the following geometry: a horizontal layer of fluid heated from below and cooled from above, with a periodic array of evenly spaced square cylinders occupying the center of the layer (whose aspect ratio is 6). Periodic boundary conditions are employed in the horizontal direction to allow for lateral freedom for the convection cells. A two-dimensional solution for unsteady natural convection is obtained, using an accurate and efficient Chebyshev spectral multi-domain methodology, for different Rayleigh numbers varying over the range of 103 to 106. In order to assess the impact of different geometric approximations on the flow structure, dynamics and overall heat transfer, the results for the above case, denoted WC (wide aspect ratio cell with periodic side boundaries and six internal bodies), are compared with those for the cases of UCNS (unit cell with no-slip adiabatic side boundaries with a single internal body), UCPC (unit cell with periodic side boundaries with a single internal body) and pure Rayleigh–Bénard convection. The results for the case of WC with six adiabatic bodies are also compared to those with six neutral isothermal bodies to consider the effects of the thermal boundary condition on time-dependent natural convection in the enclosure.
Making networked research and development pay (2007)

The idea of outsourcing offshore is synonymous with cutting costs, but organisations are forgetting that they're doing it to be competitive. As the trend to cash in on the opportunities from outsourcing to low-cost countries gathers pace, many technology-driven product companies are moving beyond traditional sourcing partnerships. An increasing number of organisations across industries are now offshoring or outsourcing significant elements of their product development work - including those capabilities that have traditionally differentiated them from the competition. This move promises to deliver more innovative products and solutions to market faster. From a financial perspective the objective is to achieve a greater return on innovation - increasing the number of new products and solutions but without a proportional increase in development costs. But the road to success is paved with difficulties. This paper discusses how companies ensure that their offshoring or outsourcing strategy actually delivers on the promise.
Digital Techniques for Wideband Receivers (2004)

This newly updated, second edition of Digital Techniques for Wideband Receivers is a current, comprehensive design guide for your digital processing work with today's complex receiver systems. Brand new material brings you up-to-date with the latest information on wideband electronic warfare receivers, the ADC testing procedure, frequency channelization and decoding schemes, and the operation of monobit receivers. The book shows you how to effectively evaluate ADCs, offers insight on building electronic warfare receivers, and describes zero crossing techniques that are critical to new receiver design. From fundamental concepts and procedures to recent technology advances in digital receivers, you get practical solutions to all your demanding wideband receiver problems. This hands-on reference is packed with 1,103 equations and 315 illustrations that support key topics covered throughout the book.
Spectroscopic study of environment-dependent changes in the conformation of the isolated carboxy-terminal telopeptide of type I collagen (1986)

The C-terminal telopeptide of the alpha 1 chain of type I collagen from bovine skin was isolated from a bacterial collagenase digest. Two forms of the telopeptide were obtained, one with two and the other with three residues of tyrosine. In both of these, the single lysyl residue had been oxidized to alpha-aminoadipic delta-semialdehyde. Circular dichroism spectra of the telopeptide in aqueous solution at neutral pH were interpreted as indicating the presence of little regular secondary structure. However, sodium dodecyl sulfate at a concentration of 40 mM induced some alpha helix, as predicted from the sequence, and trifluoroethanol also induced secondary structure, probably a mixture of alpha helix and beta sheet. A major feature of the circular dichroism spectra of the telopeptide in sodium dodecyl sulfate, in denaturing agents, and in sodium phosphate buffer at low temperature was a positive band at 227 nm due to tyrosine side-chain chromophores. The disappearance of this band on heating and at high pH was ascribed to the adoption by the telopeptide of a specific tertiary structure. Poly(ethylene glycol) 1000 used as a perturbant in UV difference spectroscopy caused conformational changes resulting in decreased accessibility of tyrosine side chains and transfer of these to a less polar environment. A structural model in which the four aromatic side chains of the telopeptide are arranged in two pairs with the rings antiparallel is proposed to account for these results.
Yb3+-doped CaF2-LaF3 Ceramic Laser (2013)

Ytterbium-doped CaF2-LaF3 transparent ceramic was synthesized from nanocrystals. 3% Yb3+-doped ceramic exhibits broadband absorption and fluorescence spectra like Yb3+:CaF2 crystal. Laser operation with 1.09 W of output power and 35% slope efficiency was obtained.
Design and Diagnosis of Wideband Coupled-Resonator Bandpass Filters (2012)

A method for circuit elements parameter extraction of wideband filters from their electromagnetic (EM) simulated response is presented. A combline-like resonator for filter realization is introduced. Several possible electric and magnetic coupling structures are investigated. The equivalent lumped element circuit has electric and magnetic couplings modeled by capacitors and inductors instead of frequency-independent reactance or impedance inverters. The parameter extraction method of direct-coupled-resonator filters is developed and is applied to symmetric canonical filters. For filters with nonadjacent spurious couplings, optimization is used to extract the lumped element parameters to reproduce the EM simulated response. Excellent agreement between the EM simulation, lumped elements extracted responses and measurements is obtained. Filter synthesis is carried out by an optimization process. Several filter designs are presented to illustrate the design procedure and validate the circuit modeling. Measured results of an eight-pole filter verified the design process.
Should Chloral Hydrate Be Banned? (1993)

The assumptions underlying current regulatory practices for environmental chemicals are not applicable to the medicinal use of chloral hydrate. Instead, a threshold model is appropriate. The data do not suggest the need to ban chloral hydrate as a medicine; however, possible modifications in its use are suggested.
Evolving microprocessors which better meet the needs of automotive electronics (1978)

The rather explosive historical progress being made in large-scale integration of semiconductor elements is moving faster than the two or three years lead time needed in the automotive industry to move a new idea into production. By presenting a forecast of what can be done soon at a developmental level, both at the automotive electronic-control level and at the LSI microcomputer single-chip development level, the writer hopes that the next generation of automotive electronics in the early 1980's can be started with a clear picture of the calculated risk-taking involved. Topics discussed are the need for custom microprocessors, input and output information rates, and applications to engine control and instrument panel display control, including the concept of satellite assemblies which exchange information available at remote locations.
Tributyrin Specifically Induces a Lipase with a Preference for the sn-2 Position of Triglyceride in <i>Geotrichum</i> sp. FO401B (2000)

The extracellular Lipases A and C produced by Geotrichum sp. FO401B have a preference for the sn-1,3 and sn-2 positions of triglyceride, respectively. Total production of these lipases was increased by plant oils and tributyrin. Butyl Toyopearl column chromatography demonstrated that only Lipase C was produced in the presence of tributyrin. Lipase C hydrolysed natural fats except sardine oil preferentially at the sn-2 position, but it showed little stereoselectivity for triolein.
The Cardiovascular Patho Physiology Of Sepsis (1989)

CD40 is a cell-surface member of the TNF (tumor necrosis factor) receptor superfamily. Upon activation, CD40 can license dendritic cells to promote antitumor T cell activation and re-educate macrophages to destroy tumor stroma. Numerous agonist CD40 ...Read More
Integrating on-chip temperature sensors into DfT schemes and BIST architectures (2002)

The continuously increasing power densities in integrated circuits necessitated the introduction of DfTT (Design for Thermal Testability) design methodology to prevent overheating effects. Newly developed CMOS temperature sensors enable the application of DfTT principle in safety-critical circuits. Parameters and operation principles of the low-power small-area temperature sensor family are presented in details in the paper, followed by the discussion of placement and testing strategies.