gebhart/patrepeval huggingface dataset:
Sequence and Localization of the Mouse Sperm Autoantigenic Protein, Sp171 (1995)

The present study characterizes the sperm protein Sp17 in the mouse. Sp17 is a mammalian testis- and sperm-specific protein that has been isolated, sequenced, and characterized from rabbit testis and spermatozoa. In this study, a rabbit Sp17 cDNA probe representing the entire protein coding region was used to screen a mouse testis cDNA library to obtain the mouse Sp17 sequence. The mouse mRNa for Sp17 encodes a 149-amino acid protein with a predicted molecular weight of 17,296. The mouse Sp17 (MSp17) cDNA sequence is 82% identical to the rabbit Sp17 cDNA sequence while the MSp17 protein sequence is 74% identical to the rabbit protein sequence. The presence of native Sp17 in mouse spermatozoa and testis was demonstrated by Western blot analysis, immunoprecipitation, and immunolocalization. After SDS-PAGE, the native Sp17 has an apparent molecular mass of 24 kDa. The sequence of the native Sp17 was confirmed by Western blots of mouse testis and spermatozoa probed with two anti-peptide antibodies--one, anti-G22C, made against amino acids 61-82 in the rabbit sequence (61-83 in the mouse), and a second, anti-K18C, made against amino acids 120-136 in the C-terminal region in the human sequence (118-134 in the mouse sequence). In the absence of proteolytic inhibitors, part of the C-terminal of native MSp17 is cleaved, giving rise to an 18-kDa band. Sp17 is present in spermatocytes and spermatids in the testis. In spermatozoa, Sp17 is not available to bind antibody on the surface of live, acrosome-intact spermatozoa, but it is present on the equatorial surface of live, acrosome-reacted spermatozoa. In fixed spermatozoa, staining is observed along the length of the principal piece, weakly along the midpiece, and over the acrosomal region of the head. When the acrosome reaction begins, acrosomal staining is seen throughout the equatorial region of the acrosome. Using mimotope analysis, this study has also demonstrated that native Sp17 is a sperm autoantigen and that recombinant mouse Sp17 is immunogenic in males with a highly restricted linear epitope.
LiveSketch: Query Perturbations for Guided Sketch-based Visual Search (2019)

LiveSketch is a novel algorithm for searching large image collections using hand-sketched queries. LiveSketch tackles the inherent ambiguity of sketch search by creating visual suggestions that augment the query as it is drawn, making query specification an iterative rather than one-shot process that helps disambiguate users' search intent. Our technical contributions are: a triplet convnet architecture that incorporates an RNN based variational autoencoder to search for images using vector (stroke-based) queries; real-time clustering to identify likely search intents (and so, targets within the search embedding); and the use of backpropagation from those targets to perturb the input stroke sequence, so suggesting alterations to the query in order to guide the search. We show improvements in accuracy and time-to-task over contemporary baselines using a 67M image corpus.
New cuts for torsional mode quartz crystal resonators (1993)

This paper describes new cuts for torsional mode quartz crystal resonators, which are called “TT(Y)-cut”. The object of this paper is to propose new cuts for torsional quartz crystal resonators with a zero temperature coefficient and to clarify their frequency characteristics, frequency temperature behavior and electrical equivalent circuit parameters. First, one shows a frequency equation that is given as a function of torsional rigidity C <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">t</sub> . Namely, the problem of a vibration for torsional mode is substantially equivalent to that of torsional rigidity C <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">t</sub> , and it is derived from stress function Ψ obtained solving a partial differential equation with respect to y and z, including elastic compliance constant s <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">56</sub> . Next, from the frequency equation numerous relationships where α reaches zero are found to exist between thickness-to-width ratio Rzy and cut angle (φ,θ), especially, the second order temperature coefficient β has a small value of -1.25×10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-8</sup> /°C <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> whose absolute value is approximately one third of the well-known flexural mode quartz crystal resonator. The value of β is then compared with the measured data -1.00×10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-8</sup> /°C <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> , so that both results are found to agree sufficiently well. Finally, it is shown that torsional quartz crystal resonators to tuning fork-type are successfully obtained with a small R <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">1</sub> of 3.5 to 4.6 k ω and a large Q value of 241,000 to 272,000 in a frequency range of about 300 to 600 kHz
A Robust DSP Integrator for Accelerometer Signals (2004)

The need for an accepted method for determining position data from digital accelerometer readings with known frequency range is very important. The method of this paper uses spectral information and provides more stability and accuracy than classic methods for the DSP case. It even reduces to a classic method for the nonoscillatory case. Examples show the robustness of the method, overcoming the instability apparent with other integration methods.
Decaprenylphosphoryl-&#946;-D-Ribose 2-Epimerase from Mycobacterium tuberculosis is a Magic Drug Target (2010)

Tuberculosis is still a leading cause of death in developing countries and a resurgent disease in developed countries. The selection and soaring spread of Mycobacterium tuberculosis multidrug-resistant (MDR-TB) and extensively drug-resistant strains (XDR-TB) is a severe public health problem. Currently, there is an urgent need of new drugs for tuberculosis treatment, with novel mechanisms of action and, moreover, the necessity to identify new drug targets. Several enzymes involved in various metabolic processes have been described as potential targets for the development of new drugs. Recently, two different classes of most promising drugs, the benzothiazinones (BTZ) and the dinitrobenzamide derivatives (DNB), have been found to be highly active against M. tuberculosis, including XDR-TB strains. Interestingly, both drugs have the same target: the heteromeric decaprenylphosphoryl-β-D-ribose 2'-epimerase encoded by dprE1 (Rv3790) and dprE2 (Rv3791) genes, respectively. DprE1 and DprE2 are involved in the biosynthesis of D-arabinose and, in particular, they are essential to perform the transformation of decaprenylphosphoryl-D-ribose to decaprenylphosphoryl-D-arabinose, which is a substrate for arabinosyltransferases in the synthesis of the cell-envelope arabinogalactan and liporabinomannan polysaccharides of mycobacteria. Arabinogalactan is a fundamental component of the mycobacterial cell wall, which covalently binds the outer layer of mycolic acids to peptidoglycan. The heteromeric decaprenylphosphoryl-β-D-ribose 2'-epimerase thus represents a valid vulnerable antimycobacterial drug target which could result in "magic" for tuberculosis treatment.
On correlations between dual-polarized base station antennas (2005)

Several diversity techniques employing multiple antennas in the base station have been developed for mobile communication systems. However, the performance of diversity methods strongly depends on the correlation between antenna elements. When multiple copolarized antennas are used in base station, the practical factor limiting the number of diversity branches is the required antenna separation, because large antenna separation makes the antenna array unfavourable large while small antenna separation may result in high correlations between the antennas. Therefore, dual-polarized antennas are commonly used to implement two diversity branches. If more than two diversity branches are needed, spatially separated dual-polarized antennas provide an attractive solution alternative. In this paper we propose a model for calculating the correlations between the cross and copolarized antennas when employing spatially separated dual-polarized antennas with nominal /spl plusmn/45/spl deg/ inclination to vertical linear polarization. Results are of theoretical nature and show the relationship between base station antenna correlations and the properties of the physical channel, base station, and mobile station antennas.
Vocabulary tree incremental indexing for scalable location recognition (2008)

This work aims at developing a scalable vision-based location recognition system where the backend database can be updated incrementally. Our proposed framework enables incremental indexing of vocabulary tree model, which efficiently includes new data into model refinement without re-generating entire model from overall dataset. An adaption trigger criterion is presented to lessen system computational cost, which is achieved by density-based relative entropy estimation between original dataset and newly coming data. Experiments on Seattle urban scene datasets with over 20K street-side images show the effectiveness of our work.
Generating contextual description from driving behavioral data (2014)

This paper presents an automatic translation method from time-series driving behavior into natural language with contextual information. Nowadays, various advanced driver-assistance systems (ADASs) have been developed to reduce the number of traffic accidents and multiple ADASs are required to reduce further accidents. For such multiple ADASs, considering the context of driving and selecting appropriate assistance is key because the systems have to handle extremely complicated driving situations consisting of drivers (and their intents and maneuvers), environments (including other traffic participants such as vehicles and pedestrians), and vehicles dynamics. In this paper, time-series driving behavior is segmented into typical driving situation symbols, and the natural language expression of each situation is generated via the behavioral feature distribution observed in each situation. Owing to the symbolization of the driving behavior, the generated behavioral descriptions can be associated with their causes and results not on an actual time axis but on a situation-symbol axis as contextual descriptions, e.g., “letting up on gas pedal to pass tollgate.” The effectiveness of the proposed method was evaluated by using an actual data set of more than eight hours over a distance of 300 km in total. Although contextual expressions are very diverse even among human drivers, the proposed method obtained an agreement of more than 70%.
Abstract 5286: Expression of cancer type amino acid transporter LAT1 is a prognosis prediction factor in breast carcinoma: comparison between triple-negative and non-triple-negative types (2015)

Abstract Purpose: Amino acid transporters play important roles in cell survival and proliferation. L-Type amino acid transporter 1 (LAT1) is one of the major Na+-independent neutral amino acid transporters. LAT1 has an aspect of oncofetal protein. Recently, high levels of LAT1 expression have been reported in various tumors, such as prostate cancer, pancreas cancer, bile duct cancer, lung cancer and stomach cancer.We revealed LAT1 expression is an independent prognosis marker in stomach cancer, prostate cancer and pancreas cancer. The purpose of the present study was to investigate the LAT1 expression in breast carcinoma and relationship between LAT1 expression and prognosis. Materials & Methods: We enrolled 290 patients with invasive breast carcinoma surgically resected between 2006 and 2010 at Kitasato University Hospital and Toho University Omori Medical Center, Japan. We investigated LAT1 expression in the enrolled breast carcinoma cases by immunohistochemical staining. All cases were divided into two groups, 78 cases of triple negative carcinoma (TNBC) and 212 cases of non-triple negative carcinoma (NTNBC), with immunohistochemistry of estrogen and progesterone receptors and Her2. After LAT1 expression was compared between TNBC and NTNBC, correlations among LAT1 expression, Ki-67 labeling index (LI), CD98 expression, and clinicopathological findings was assessed. Results: TNBC showed significantly higher LAT1 expression than NTNBC. TNBC also showed significantly higher CD98 expression rather than NTNBC. LAT1 expression of extraductal invasive cancer cells was significantly higher than those of intraductal carcinoma cells. A significantly positive correlation was observed between LAT1 expression and CD98 expression. Further, a positive correlation between LAT1 expression and Ki-67 LI was found. Log-rank test in overall breast cancers revealed that high LAT1 expression group showed significantly poor prognosis, compared to low LAT1 group. In NTNBC, high LAT1expression cases possessed poorer prognosis than low LAT1 expression cases. Conclusion: In invasive ductal carcinoma of breast, LAT1 expression has a potential for a prognosis prediction factor. LAT1 offers a potential target for anti-cancer therapy by its inhibitor JPH203 which has been developed by us. Citation Format: Masaaki Ichinoe, Tetsuo Mikami, Kiyomi Hana, Nobuyuki Yanagisawa, Hitoshi Endou, Isao Okayasu, Yoshiki Murakumo. Expression of cancer type amino acid transporter LAT1 is a prognosis prediction factor in breast carcinoma: comparison between triple-negative and non-triple-negative types. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 5286. doi:10.1158/1538-7445.AM2015-5286
Linewidth reduction of a broad-area laser diode array in a compound external cavity (2009)

A compound external cavity is designed and implemented to achieve a homogeneous spectrum distribution of broad area laser diodes in an array and to narrow the spectral linewidth of the entire array. The compound external cavity is composed of an optical coupler and a Littrow external cavity with a telescope along the fast axis. The inhomogeneous distribution of individual laser diodes spectrum generated by the "smile" effect was reduced by the telescope. The effective transverse coupling among individual laser diodes in an array was enhanced by the optical coupler, which further reduced the spectrum inhomogeneous distribution of the entire array. The spectral linewidth of a 49-emitter laser array is reduced to 0.1 nm at the output power of 12.5 W.
Efficient Aziridination of Olefins Catalyzed by Mixed‐Valent Dirhodium(II,III) Caprolactamate. (2005)

Abstract For Abstract see ChemInform Abstract in Full Text.
Analytical formulation and experimental study of the properties of piezopolymer transducers using shell-or membrane-dome structures (1982)

Dome shaped piezopolymer transducers can be considered as membrane or shell structures, depending on the thickness of the thermoformed film. We report an analysis of the mechanical behavior of such structures, taking into account both the extensional and flexural effects, and leading to a formulation of their acoustic mass and compliance and of the frequency of their first resonance mode. The microphone sensitivity and the transmitter efficiency are then, respectively, derived from the stress field induced by an applied pressure and from the relative change of radius of curvature due to an applied electrical field. These predictions are more specifically applied to the optimization of telephone microphones and receivers. It is shown that the product of the microphone sensitivity by the square of the resonance frequency and the radius of curvature is a function of material parameters only. Maximal receiver efficiency is ascribed to the piezoelectric coupling factor of the material. Experimental results will be presented, concerning such transducers using home made piezopolymer PVF2 in shell or membrane structures. [Work sponsored by DAII.]
Molecular Characterization and Expression of Cloned Human Galanin Receptors GALR2 and GALR3 (1998)

Galanin is a 29- or 30-amino acid peptide with wide-ranging effects on hormone release, feeding behavior, smooth muscle contractility, and somatosensory neuronal function. Three distinct galanin receptor (GALR) subtypes, designated GALR1, 2, and 3, have been cloned from the rat. We report here the cloning of the human GALR2 and GALR3 genes, an initial characterization of their pharmacology with respect to radioligand binding and signal transduction pathways, and a profile of their expression in brain and peripheral tissues. Human GALR2 and GALR3 show, respectively, 92 and 89% amino acid sequence identity with their rat homologues. Radioligand binding studies with 125I-galanin show that recombinant human GALR2 binds with high affinity to human galanin (K(D) = 0.3 nM). Human GALR3 binds galanin with less affinity (IC50 of 12 nM for porcine galanin and 75 nM for human galanin). Human GALR2 was shown to couple to phospholipase C and elevation of intracellular calcium levels as assessed by aequorin luminescence in HEK-293 cells and by Xenopus melanophore pigment aggregation and dispersion assays, in contrast to human GALR1 and human GALR3, which signal predominantly through inhibition of adenylate cyclase. GALR2 mRNA shows a wide distribution in the brain (mammillary nuclei, dentate gyrus, cingulate gyrus, and posterior hypothalamic, supraoptic, and arcuate nuclei), and restricted peripheral tissue distribution with highest mRNA levels detected in human small intestine. In comparison, whereas GALR3 mRNA was expressed in many areas of the rat brain, there was abundant expression in the primary olfactory cortex, olfactory tubercle, the islands of Calleja, the hippocampal CA regions of Ammon's horn, and the dentate gyrus. GALR3 mRNA was highly expressed in human testis and was detectable in adrenal gland and pancreas. The genes for human GALR2 and 3 were localized to chromosomes 17q25 and 22q12.2-13.1, respectively.
Polo-like Kinase 2 (PLK2) Phosphorylates α-Synuclein at Serine 129 in Central Nervous System (2008)

Several neurological diseases, including Parkinson disease and dementia with Lewy bodies, are characterized by the accumulation of alpha-synuclein phosphorylated at Ser-129 (p-Ser-129). The kinase or kinases responsible for this phosphorylation have been the subject of intense investigation. Here we submit evidence that polo-like kinase 2 (PLK2, also known as serum-inducible kinase or SNK) is a principle contributor to alpha-synuclein phosphorylation at Ser-129 in neurons. PLK2 directly phosphorylates alpha-synuclein at Ser-129 in an in vitro biochemical assay. Inhibitors of PLK kinases inhibited alpha-synuclein phosphorylation both in primary cortical cell cultures and in mouse brain in vivo. Finally, specific knockdown of PLK2 expression by transduction with short hairpin RNA constructs or by knock-out of the plk2 gene reduced p-Ser-129 levels. These results indicate that PLK2 plays a critical role in alpha-synuclein phosphorylation in central nervous system.
Selective CO<sub>2</sub> Sequestration with Monolithic Bimodal Micro/Macroporous Carbon Aerogels Derived from Stepwise Pyrolytic Decomposition of Polyamide-Polyimide-Polyurea Random Copolymers (2017)

Polymeric aerogels (PA-xx) were synthesized via room-temperature reaction of an aromatic triisocyanate (tris(4-isocyanatophenyl) methane) with pyromellitic acid. Using solid-state CPMAS <sup>13</sup>C and <sup>15</sup>N NMR, it was found that the skeletal framework of PA-xx was a statistical copolymer of polyamide, polyurea, polyimide, and of the primary condensation product of the two reactants, a carbamic-anhydride adduct. Stepwise pyrolytic decomposition of those components yielded carbon aerogels with both open and closed microporosity. The open micropore surface area increased from <15 m<sup>2</sup> g<sup>-1</sup> in PA-xx to 340 m<sup>2</sup> g<sup>-1</sup> in the carbons. Next, reactive etching at 1,000 °C with CO<sub>2</sub> opened access to the closed pores and the micropore area increased by almost 4× to 1150 m<sup>2</sup> g<sup>-1</sup> (out of 1750 m<sup>2</sup> g<sup>-1</sup> of a total BET surface area). At 0 °C, etched carbon aerogels demonstrated a good balance of adsorption capacity for CO<sub>2</sub> (up to 4.9 mmol g<sup>-1</sup>), and selectivity toward other gases (via Henry's law). The selectivity for CO<sub>2</sub> versus H<sub>2</sub> (up to 928:1) is suitable for precombustion fuel purification. Relevant to postcombustion CO<sub>2</sub> capture and sequestration (CCS), the selectivity for CO<sub>2</sub> versus N<sub>2</sub> was in the 17:1 to 31:1 range. In addition to typical factors involved in gas sorption (kinetic diameters, quadrupole moments and polarizabilities of the adsorbates), it is also suggested that CO<sub>2</sub> is preferentially engaged by surface pyridinic and pyridonic N on carbon (identified with XPS) in an energy-neutral surface reaction. Relatively high uptake of CH<sub>4</sub> (2.16 mmol g<sup>-1</sup> at 0 °C/1 bar) was attributed to its low polarizability, and that finding paves the way for further studies on adsorption of higher (i.e., more polarizable) hydrocarbons. Overall, high CO<sub>2</sub> selectivities, in combination with attractive CO<sub>2</sub> adsorption capacities, low monomer cost, and the innate physicochemical stability of carbon render the materials of this study reasonable candidates for further practical consideration.
Efficient Pig Counting in Crowds with Keypoints Tracking and Spatial-aware Temporal Response Filtering (2020)

Pig counting is a crucial task for large-scale pig farming. Pigs are usually visually counted by human. But this process is very time-consuming and error-prone. Few studies in literature developed automated pig counting method. The existing works only focused on pig counting using single image, and its level of accuracy faced challenges due to pig movements, occlusion and overlapping. Especially, the field of view of a single image is very limited, and could not meet the needs of pig counting for large pig grouping houses. Towards addressing these challenges, we presented a real-time automated pig counting system in crowds using only one monocular fisheye camera with an inspection robot. Our system showed that it achieved performance superior to human. Our pipeline began with a novel bottom-up pig detection algorithm to avoid false negatives due to overlapping, occlusion and deformable pig shapes. This detection included a deep convolution neural network (CNN) for pig body part keypoints detection and the keypoints association method to identify individual pigs. It then employed an efficient on-line tracking method to associate pigs across image frames. Finally, pig counts were estimated by a novel spatial-aware temporal response filtering (STRF) method to suppress false positives caused by pig or camera movements or tracking failures. The whole pipeline has been deployed in an edge computing device, and demonstrated the effectiveness.
Quantum-scheme for improving interferometric visibility with imperfect distributed entangled-states (2017)

We analyze a quantum-scheme for measuring interferometric visibility for telescopic applications with imperfectly entangled states as a resource. The scheme overcomes photon loss thus permitting measurement with larger baseline leading to finer resolution of angular intensity distribution of remote sources of light.