gebhart/patrepeval huggingface dataset:
STRETCHABLE Lab-on-Chip Device With Impedance Spectroscopy Capability for Mammalian Cell Studies (2016)

This paper presents the fabrication and testing of electric cell-substrate impedance spectroscopy (ECIS) electrodes on a stretchable membrane. This is the first time when ECIS electrodes were fabricated on a stretchable substrate and ECIS measurements on mammalian cells exposed to cyclic strain of 10% were successfully demonstrated. A chemical was used to form strong chemical bond between gold electrodes of ECIS sensor and polymer membrane, which enable the electrodes keep good conductive ability during cyclic stretch. The stretchable membrane integrated with the ECIS sensor can simulate and replicate the dynamic environment of organism and enable the analysis of the cells activity involved in cells attachment and proliferation in vitro. Bovine aortic endothelial cells (BAEC) were used to evaluate the endothelial function influenced by mechanical stimuli in this research because they undergo in vivo cyclic physiologic elongation produced by the blood circulation in the arteries.
Photocatalyst and its uses (1982)

Patent number: 82-810037-57669 Reference LPI-PATENT-1982-003Search for this record at the European Patent Office Record created on 2006-02-21, modified on 2017-05-12
Ergoline congeners as potential inhibitors of prolactin release 2 (1976)

In our attempts to elucidate the prolactin release inhibiting pharmacophore within the ergoline structure, we have prepared one indolealkylamine and several 2-aminotetralin derivatives. These congeners have been evaluated for inhibition of prolactin release in vivo. One congener, 5,8-dihydroxy-2-dimethylaminotetralin, and the drug M-7 significantly inhibited prolactin secretion.
Low-Dispersion Turns and Junctions for Microchannel Systems (2000)

Numerical methods are employed to optimize the geometry of two-dimensional microchannel turns such that the turn-induced spreading of a solute band is minimized. An inverted numerical method is first developed to compute the electric potential and local species motion in turns of arbitrary geometry. The turn geometry is then optimized by means of a nonlinear least-squares minimization algorithm using the spatial variance of the species distribution leaving the turn as the object function. This approach yields the turn geometry producing the minimum possible dispersion, subject only to prescribed constraints. The resulting low-dispersion turns provide an induced variance 2-3 orders of magnitude below that of a comparable conventional turns. Sample results are presented for 180 and 90 degrees turns, and the use of these turns to form wyes and tees is discussed. A sample 45 degrees wye is presented. The use of low-dispersion turns in folding separation columns is also discussed, and sample calculations are presented for folding a column 100 microm in width and up to 900 mm in length onto a region of only 10 by 10 mm. These low-dispersion geometries are applicable to electroosmosis, electrophoresis, and some pressure-driven flows.
Three-dimensional integral imaging with electronically synthesized lenslet arrays (2002)

We propose to use electronically synthesized lenslet arrays in three-dimensional integral imaging, which will permit control of lenslet positions and characteristics in real time. We can then potentially achieve improvements in the quality of reconstructured three-dimensional images by adopting a moving-lenslet-array technique with no mechanical movement of optical components. We present preliminary experiments to show the feasibility of our approach with an off-the-shelf liquid-crystal display.
Catalytic Oxidations of Steroid Substrates by Artificial Cytochrome P-450 Enzymes (2002)

Catalysts comprising manganese-porphyrins carrying cyclodextrin binding groups are able to perform hydroxylations with substrate selectivity and regio- and stereoselectivity and high catalytic turnovers. The geometries of the catalyst/substrate complexes override intrinsic substrate reactivities, permitting attack on geometrically accessible saturated carbons of steroids in the presence of secondary carbinol groups and carbon-carbon double bonds, as in enzymatic reactions. Selective hydroxylations of steroid carbon 9 positions are of particular practical interest.
MEMS micromirror based X-ray pulse modulators (2012)

We describe a MEMS micromirror for modulation of X-ray pulses needed for critical time-resolved process studies of cyclical and far-from-equilibrium phenomena. This MEMS micromirror leverages grazing-angle reflection of X-ray pulses to generate its temporal modulation response. The generated responses are 1.5μs wide for 8keV X-rays and have been successfully used to isolate single X-ray pulses separated 1.59μs from their suppressed neighboring pulses.
Multicavity Fabry-Pe/spl acute/rot interferometric thin-film sensor with built-in temperature compensation (2005)

A fiber-optic multicavity Fabry-Pe/spl acute/rot interferometric thin-film sensor with built-in temperature compensation has been constructed and demonstrated. Temperature information can be extracted from the multicavity structure to correct the temperature dependence of the optical thickness measurement of self-assembled thin-films. Experimental results demonstrate that the sensor is able to automatically compensate the temperature-induced optical-thickness error over the range from 0/spl deg/C to 100/spl deg/C.
Diffractive optical variable image devices generated by maskless interferometric lithography for optical security (2011)

In optical security (protection against forgery and counterfeit of products and documents) the problem is not exact reproduction but the production of something sufficiently similar to the original. Currently, Diffractive Optically Variable Image Devices (DOVID), that create dynamic chromatic effects which may be easily recognized but are difficult to reproduce, are often used to protect important products and documents. Well known examples of DOVID for security are 3D or 2D/3D holograms in identity documents and credit cards. Others are composed of shapes with different types of microstructures yielding by diffraction to chromatic dynamic effects. A maskless interferometric lithography technique to generate DOVIDs for optical security is presented and compared to traditional techniques. The approach can be considered as a self-masking focused holography on planes tilted with respect to the reference optical axes of the system, and is based on the Scheimpflug and Hinge rules. No physical masks are needed to ensure optimum exposure of the photosensitive film. The system built to demonstrate the technique relies on the digital mirrors device MOEMS technology from Texas Instruments' Digital Light Processing. The technique is linear on the number of specified colors and does not depend either on the area of the device or the number of pixels, factors that drive the complexity of dot-matrix based systems. The results confirmed the technique innovation and capabilities in the creation of diffractive optical elements for security against counterfeiting and forgery.
Identification, production and assessment of two<i>Toxoplasma gondii</i>recombinant proteins for use in a<i>Toxoplasma</i>IgG avidity assay (2016)

The IgG avidity assay is an important tool in the management of suspected toxoplasmosis in pregnant women. This study aimed to produce new Toxoplasma gondii recombinant proteins and to assess their usefulness in an IgG avidity assay. Toxoplasma positive and negative serum samples were used, the former were categorized into low (LGA) and high (HGA) IgG avidity samples. Immunoblots were performed on 30 T. gondii cDNA clones to determine the reactivity and IgG avidity to the expressed proteins. Two of the clones were found to have diagnostic potential and were analyzed further; AG12b encoded T. gondii apical complex lysine methyltransferase (AKMT) protein and AG18 encoded T. gondii forkhead-associated (FHA) domain-containing protein. The His-tagged recombinant proteins, rAG12b and rAG18, were expressed and tested with LGA and HGA samples using an IgG avidity western blot and ELISA. With the IgG avidity western blot, rAG12b identified 86.4% of LGA and 90.9% of HGA samples, whereas rAG18 identified 81.8% of both LGA and HGA samples. With the IgG avidity ELISA, rAG12b identified 86.4% of both LGA and HGA samples, whereas rAG18 identified 77.3% of LGA and 86.4% of HGA serum samples. This study showed that the recombinant antigens were able to differentiate low avidity and high avidity serum samples, suggesting that they are potential candidates for use in the Toxoplasma IgG avidity assay.
COMPACT: Flow-Based Computing on Nanoscale Crossbars with Minimal Semiperimeter (2021)

In-memory computing is a promising solution strategy for data-intensive applications to circumvent the von Neumann bottleneck. Flow-based computing is the concept of performing in-memory computing using sneak paths in nanoscale crossbar arrays. The limitation of previous work is that the resulting crossbar representations have large dimensions. In this paper, we present a framework called COMPACT for mapping Boolean functions to crossbar representations with minimal semiperimeter (the number of wordlines plus bitlines). The COMPACT framework is based on an analogy between binary decision diagrams (BDDs) and nanoscale memristor crossbar arrays. More specifically, nodes and edges in a BDD correspond to wordlines/bitlines and memristors in a crossbar array, respectively. The relation enables a function represented by a BDD with <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">$n$</tex> nodes and an odd cycle transversal of size <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">$k$</tex> to be mapped to a crossbar with a semiperimeter of n+k. The <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">$k$</tex> extra wordlines/bitlines are introduced due to crossbar connection constraints, i.e. wordlines (bitlines) cannot directly be connected to wordlines (bitlines). For multi-input multi-output functions, COMPACT can also be applied to shared binary decision diagrams (SBDDs), which further reduces the size of the crossbar representations. Compared with the state-of-the-art mapping technique, the semiperimeter is reduced from 2.13n to 1.09n on the average, which translates into crossbar representations with 78% smaller area. The power consumption and the computation delay are on the average reduced by 7% and 52%, respectively.
Modeling Contextual Modulation of Memory Associations in the Hippocampus (2018)

We present a computational model of how memories can be contextually acquired and recalled in the hippocampus. Our adaptive contextual memory model comprises the lateral entorhinal cortex (LEC), the dentate gyrus (DG) and areas CA3 and CA1 in the hippocampus, and assumes external inputs about context that originate in the prefrontal cortex (PFC). Specifically, we propose that there is a top-down bias on the excitability of cells in the DG of the hippocampus that recruits a sub-population of cells to differentiate contexts, independent of experienced stimuli, expanding the "pattern separation" role typically attributed to the DG. It has been demonstrated in rats that if PFC is inactivated, both acquisition and recall of memory associations are impaired. However, PFC inactivation during acquisition of one set of memory associations surprisingly leads to subsequent facilitation of the acquisition of a conflicting set of memory associations in the same context under normal PFC operation. We provide here the first computational and algorithmic account of how the absence or presence of the top-down contextual biases on the excitability of DG cells during different learning phases of these experiments explains these data. Our model simulates PFC inactivation as the loss of inhibitory control on DG, which leads to full or partial activation of DG cells related to conflicting memory associations previously acquired in different contexts. This causes context-inappropriate memory traces to become active in the CA3 recurrent network and thereby the output CA1 area within the hippocampus. We show that these incongruous memory patterns proactively interfere with and slow the acquisition of new memory associations. Further, we demonstrate that pattern completion within CA3 in response to a partial cue for the recall of previously acquired memories is also impaired by PFC inactivation for the same reason. Pre-training the model with interfering memories in contexts different from those used in the experiments, simulating a lifetime of experiences, was crucial to reproduce the rat behavioral data. Finally, we made several testable predictions based on the model that suggest future experiments to deepen our understanding of brain-wide memory processes.
Characterization of a Mouse/Human Chimeric Monoclonal Antibody (Cβ1) to a Principal Neutralizing Domain of the Human Immunodeficiency Virus Type 1 Envelope Protein (1992)

A chimeric mouse-human antibody (C beta 1) was constructed that recognized the principal neutralizing domain (PND) of human immunodeficiency virus type 1 (HIV-1) gp120. The constant (C) immunoglobulin regions (C gamma 1 and C kappa) of a mouse monoclonal antibody, 0.5 beta, were substituted for the human C gamma 1 and C kappa by recombining the DNA modules encoding variable or C regions. The DNA constructs were then transfected into X63 Ag8.653 myeloma cells. A clone with a high production of the chimeric antibody (C beta 1) was selected. This antibody was tested for its biological activity against HIV-1. It bound to the surface of HTLV-IIIB-infected cells and reacted with gp120/160 with equal affinity and specificity to that of the parental 0.5 beta murine monoclonal antibody in a Western blot assay. Neutralization and/or enhancement of HIV infection were evaluated with C beta 1 and 0.5 beta. Both C beta 1 and 0.5 beta neutralized cell-to-cell infection and cell-free virus infection by HTLV-IIIB. Antibody-dependence enhancement of HIV infection was not observed with either C beta 1 or 0.5 beta in the presence or absence of human complement. Antibody-dependent cell-mediated cytolysis (ADCC) and antibody-dependent complement-mediated cytolysis (ACC) were observed with C beta 1 but not with the parental 0.5 beta. These findings suggest that the neutralizing antibodies to PND may neutralize but not enhance HIV infection. Furthermore, the high levels of ACC and ADCC shown against HIV-infected cells by C beta 1 indicate that the clinical application of such monoclonal antibodies may be possible.
Extending Segment Routing into Optical Networks (2017)

New extensions to Segment Routing are introduced here that allow for an end-to-end path to include optical transport network segments that steer packets across optical networks for maximal performance with minimal operational changes.
Novel Role of MDA-9/Syntenin in Regulating Urothelial Cell Proliferation by Modulating EGFR Signaling (2013)

Our findings indicate that MDA-9/Syntenin might provide an attractive target for developing detection, monitoring, and therapeutic strategies for managing UCC.
Connectivity-Guided Predictive Compression of Dynamic 3D Meshes (2006)

We introduce an efficient algorithm for real-time compression of temporally consistent dynamic 3D meshes. The algorithm uses mesh connectivity to determine the order of compression of vertex locations within a frame. Compression is performed in a frame to frame fashion using only the last decoded frame and the partly decoded current frame for prediction. Following the predictive coding paradigm, local temporal and local spatial dependencies between vertex locations are exploited. In this framework we present a novel angle preserving predictor and evaluate its performance against other state of the art predictors. It is shown that the proposed algorithm improves up to 25% upon the current state of the art for compression of temporally consistent dynamic 3D meshes.
A Large Internal Deletion Converts Yeast LEU3 to a Constitutive Transcriptional Activator (1989)

LEU3 of Saccharomyces cerevisiae encodes an 886-amino-acid polypeptide that activates transcription of at least five genes by binding to an upstream decanucleotide sequence. This activation is dependent on the inducer alpha-isopropylmalate, the synthesis of which is repressed by leucine. We created a 285-amino-acid LEU3 derivative by removing a large block of internal sequences, including a dense cluster of acidic residues. This deletion protein bound to the decanucleotide sequence in vitro and activated gene expression in vivo. In contrast to wild-type LEU3, the truncated LEU3 protein was an effective transcriptional activator when alpha-isopropylmalate synthesis was repressed by leucine.
Selection and Characterization of DNA Aptamers for Electrochemical Biosensing of Carbendazim (2017)

This article reports a novel aptamer-based impedimetric detection of carbendazim, a commonly used benzimidazole fungicide in agriculture. High affinity and specificity DNA aptamers against carbendazim were successfully selected using systematic evolution of ligand by exponential enrichment (SELEX). The dissociation constants (K<sub>d</sub>s) of the selected DNA aptamers after 10 in vitro selection cycles were characterized using fluorescence-based assays showing values in the nanomolar range. The aptamer which showed the highest degree of affinity and conformation change was used to fabricate an electrochemical aptasensor via self-assembly of thiol-modified aptamer on gold electrodes. The aptasensor exploits the specific recognition of carbendazim by the aptamer immobilized on the gold surface which leads to conformational changes in the aptamer structure. This conformational change alters the access of a ferrocyanide/ferricyanide redox couple to the aptasensor surface. The aptasensor response is thus measured by following the increase in the electron transfer resistance of the redox couple using Faradaic electrochemical impedance spectroscopy. This method allowed a selective and sensitive label-free detection of carbendazim within a range of 10 pg/mL-10 ng/mL with a limit of detection of 8.2 pg/mL. The aptasensor did not show cross reactivity with other commonly used pesticides such as fenamiphos, isoproturon, atrazine, linuron, thiamethoxam, trifluralin, carbaryl, and methyl parathion. Moreover, the aptasensor has been applied in different spiked food matrixes showing high recovery percentages. We believe that the proposed aptasensor is a promising alternative to the currently used methods for carbendazim monitoring.
Locomotive micro-implant with active electromagnetic propulsion (2009)

An active locomotive technique requiring only an external power source and a static magnetic field is presented, and its operation is analyzed and simulated. For a modest static MRI magnetic field of 1 T, the results show that a 1-mm cube achieves roughly 3 cm/sec of lateral motion using less than 20.4 microW of power. Current-carrying wires generate the forces, resulting in highly controllable motion. Existing solutions trade off size and power: passive solutions are small but impractical, and mechanical solutions are inefficient and large. The presented solution captures the advantages of both systems, and has much better scalability.
Intracellular O<sub>2</sub> Sensing Probe Based on Cell-Penetrating Phosphorescent Nanoparticles (2011)

A new intracellular O(2) (icO(2)) sensing probe is presented, which comprises a nanoparticle (NP) formulation of a cationic polymer Eudragit RL-100 and a hydrophobic phosphorescent dye Pt(II)-tetrakis(pentafluorophenyl)porphyrin (PtPFPP). Using the time-resolved fluorescence (TR-F) plate reader set-up, cell loading was investigated in detail, particularly the effects of probe concentration, loading time, serum content in the medium, cell type, density, etc. The use of a fluorescent analogue of the probe in conjunction with confocal microscopy and flow cytometry analysis, revealed that cellular uptake of the NPs is driven by nonspecific energy-dependent endocytosis and that the probe localizes inside the cell close to the nucleus. Probe calibration in biological environment was performed, which allowed conversion of measured phosphorescence lifetime signals into icO(2) concentration (μM). Its analytical performance in icO(2) sensing experiments was demonstrated by monitoring metabolic responses of mouse embryonic fibroblast cells under ambient and hypoxic macroenvironment. The NP probe was seen to generate stable and reproducible signals in different types of mammalian cells and robust responses to their metabolic stimulation, thus allowing accurate quantitative analysis. High brightness and photostability allow its use in screening experiments with cell populations on a commercial TR-F reader, and for single cell analysis on a fluorescent microscope.
Unitary space-time codes and the Cayley transform (2002)

A recently proposed method for communicating with multiple antennas over block fading channels is unitary space-time modulation (USTM), so-called because the transmitted signals form a matrix with orthonormal columns. Since channel knowledge is not required at the receiver, USTM schemes are suitable for use on wireless links where channel tracking is undesirable or infeasible. Recent results have shown that, if suitably designed, USTM schemes can achieve full channel capacity at high SNR. While all this is well recognized, what is not clear is how to generate good performing constellations of (non-square) unitary matrices, that lend themselves to efficient encoding/decoding. The schemes proposed so far either exhibit poor performance, especially at high rates, or have no efficient decoding algorithms. In this paper, we propose to use the Cayley transform to design USTM constellations. This work is a generalization, to the non-square case, of the Cayley codes that have been proposed for differential USTM. The codes are designed based on an information-theoretic criterion, and lend themselves to polynomial-time (often cubic) near-maximum-likelihood decoding using a sphere decoding algorithm.
Silicon based antennas for THz integrated arrays (2011)

A set of antenna geometries for future integrated arrays at terahertz frequencies, based on two fabrication techniques is presented. The first technique consists in an advanced laser micro-fabrication, allowing fabricating advanced 3D geometries but may not be cost-efficient for large arrays. The second one is based in photolithographic processes, allowing the fabrication of arrays on a single wafer in parallel.
A bandwidth-efficient scheme for non-coherent transmit diversity (2004)

Differential transmit diversity which can be detected noncoherently is an attractive alternative to coherent space-time block codes since channel estimation is more difficult in multi-antenna systems than in single antenna systems. We focus on differential space-time block codes from orthogonal designs which derive the transmit symbols from an M-PSK constellation in order to fulfill the requirement that the transmit vectors have unit length. Since PSK is only advantageous for M /spl les/ 8, we propose to extend the transmit symbol constellation for bandwidth-efficient transmission such that multiple levels are allowed for the length of the transmit vector. Furthermore, we derive a simple soft-output detector which does not require channel estimation. The new scheme outperforms the existing unit-length approach at high bandwidth-efficiency, particularly in time-varying channels. Furthermore, it is more flexible in terms of data rate adaptation and has lower detection complexity.