gebhart/patrepeval huggingface dataset:
Development of an Intravascular Lung Assist Device (1989)

Intravascular lung assist devices (ILADs) must provide sufficient gas transport without excessive pressure drop. In vitro studies of fiber-bundle devices determined the effect of geometric configuration on gas transfer efficiency, the amount of surface area needed to achieve sufficient gas transport, and the relationship between gas transport and blood-side pressure drop. ILADs with cross-flow fibers transfer at least 10 times more O2 than parallel flow ILADs of the same surface area, and six to eight times more CO2. Some tested devices with 0.4-0.6 m2 of surface area can transfer 100 ml/min of both O2 and CO2. Configurations with high gas transport, however, often require moderate pressure drops.
Efficient Fluoride‐Mediated Synthesis of 5‐Alkyl Amino‐ and Ether‐Substituted Pyrazoles (2005)

Abstract For Abstract see ChemInform Abstract in Full Text.
Characterization of Molecular Features, Antigen-Binding, and <i>in Vitro</i> Properties of IgG and IgM Variants of 4E10, an Anti-HIV Type 1 Neutralizing Monoclonal Antibody (2004)

The human monoclonal antibody 4E10 has been generated previously by immortalization of peripheral blood cells from an HIV-1-infected individual. This antibody binds to the linear epitope NWFDIT on gp41 and exhibits exceptional neutralizing activity against a broad spectrum of primary HIV-1 isolates. In the present study, molecular features, immunoreactivity, and functional activity of 4E10 were studied. The original hybridoma-derived 4E10 was of subtype IgG(3). Analysis of the variable segment of the heavy chain (VH) demonstrated extensive somatic mutations compared to the closest homologous germline gene VH1-69. Most amino acid substitutions occurred in the complementarity-determining region (CDR) 2, characteristic for an antigen-driven somatic maturation. The heavy chain of the CDR3 (H3) is of unusual length and cannot be attributed with certainty to any specific D(H) locus. To enable mass production and to prolong the in vivo half-life, 4E10 was subsequently cloned as IgG(1) in Chinese hamster ovary (CHO) cells. In additional studies, 4E10 was class switched to the IgM isotype. Binding to the linear epitope NWFDIT was not significantly changed after the cloning procedures. However, in vitro studies revealed dramatic differences in the neutralizing potential. The antiviral activity could be greatly enhanced by change of IgG(3) to IgG(1). In contrast, the IgM isotype almost completely lost its neutralizing potential.
Abstract A007: Intratumoral delivery of inactivated vaccinia virus is more efficacious than live oncolytic vaccinia virus in murine bilateral tumor implantation models (2016)

Abstract Poxviruses are large cytoplasmic DNA viruses that have potentials as vaccine vectors and oncolytic agents. Although almost all of the oncolytic vaccinia viruses that are being investigated in clinical trials are replication competent, it is still unclear how these viruses exert antitumor immunologic effects, and whether viral replication is even necessary to achieve systemic antitumor efficacy. In this study, we compared therapeutic efficacy of the intratumoral delivery of the live, attenuated, replication competent oncolytic vaccinia virus expressing mGM-CSF (VC-TK−-mGM-CSF)vs. its heat-inactivated counterpart (iVC-TK−-mGM-CSF) in two bilateral tumor implantation models- B16-F10 murine melanoma and MC38 colon adenocarcinoma. We found that intratumoral injection of inactivated virus exerted stronger systemic antitumor effects than the live virus. FACS analyses of tumor infiltrating immune cells revealed that intratumoral injection of iVC-TK−-mGM-CSF lead to increased percentages and numbers of activating effector CD8+ and CD4+ T cells and reduced percentages and numbers of CD4+Foxp3+ regulatory T cells in the non-injected tumors compared with intratumoral delivery of the live virus. Infection of bone marrow-derived dendritic cells (DCs) with iVC-TK−-mGM-CSF induced type I IFN and proinflammatory cytokine and chemokine production and DC maturation, whereas the live virus did not. Taken together, our results demonstrate that intratumoral delivery of inactivated vaccina virus is safer and more efficacious than live oncolytic vaccinia virus expressing mGM-CSF in two murine bilateral tumor implantation models. Our findings have important implications for the design of poxviral-based immunotherapeutics for patients with metastatic cancers. Note: This abstract was not presented at the conference. Citation Format: Weiyi Wang, Peihong Dai, Ning Yang, Stewart Shuman, Taha Merghoub, Jedd D. Wolchok, Liang Deng. Intratumoral delivery of inactivated vaccinia virus is more efficacious than live oncolytic vaccinia virus in murine bilateral tumor implantation models [abstract]. In: Proceedings of the Second CRI-CIMT-EATI-AACR International Cancer Immunotherapy Conference: Translating Science into Survival; 2016 Sept 25-28; New York, NY. Philadelphia (PA): AACR; Cancer Immunol Res 2016;4(11 Suppl):Abstract nr A007.
Gaussian Belief Propagation for Solving Systems of Linear Equations: Theory and Application (2008)

The canonical problem of solving a system of linear equations arises in numerous contexts in information theory, communication theory, and related fields. In this contribution, we develop a solution based upon Gaussian belief propagation (GaBP) that does not involve direct matrix inversion. The iterative nature of our approach allows for a distributed message-passing implementation of the solution algorithm. We address the properties of the GaBP solver, including convergence, exactness, computational complexity, message-passing efficiency and its relation to classical solution methods. We use numerical examples and applications, like linear detection, to illustrate these properties through the use of computer simulations. This empirical study demonstrates the attractiveness (e.g., faster convergence rate) of the proposed GaBP solver in comparison to conventional linear-algebraic iterative solution methods.
Synthesis and Biological Evaluation of 3‐Arylindazoles as Selective MEK4 Inhibitors (2019)

Herein we report the discovery of a novel series of highly potent and selective mitogen-activated protein kinase kinase 4 (MEK4) inhibitors. MEK4 is an upstream kinase in MAPK signaling pathways that phosphorylates p38 MAPK and JNK in response to mitogenic and cellular stress queues. MEK4 is overexpressed and induces metastasis in advanced prostate cancer lesions. However, the value of MEK4 as an oncology target has not been pharmacologically validated because selective chemical probes targeting MEK4 have not been developed. Optimization of this series via structure-activity relationships and molecular modeling led to the identification of compound 6 ff (4-(6-fluoro-2H-indazol-3-yl)benzoic acid), a highly potent and selective MEK4 inhibitor. This series of inhibitors is the first of its kind in both activity and selectivity and will be useful in further defining the role of MEK4 in prostate and other cancers.
An investigation of the feasibility of gadolinium for neutron capture synovectomy (2000)

Neutron capture synovectomy (NCS) has been proposed as a possible treatment modality for rheumatoid arthritis. Neutron capture synovectomy is a two-part modality, in which a compound containing an isotope with an appreciable thermal neutron capture cross section is injected directly into the joint, followed by irradiation with a neutron beam. Investigations to date for NCS have focused on boron neutron capture synovectomy (BNCS), which utilizes the 10B(n,alpha)7Li nuclear reaction to deliver a highly localized dose to the synovium. This paper examines the feasibility of gadolinium, specifically 157Gd, as an alternative to boron as a neutron capture agent for NCS. This alternative modality is termed Gadolinium Neutron Capture Synovectomy, or GNCS. Monte Carlo simulations have been used to compare 10B and 157Gd as isotopes for accelerator-based NCS. The neutron source used in these calculations was a moderated spectrum from the 9Be(p,n) reaction at a proton energy of 4 MeV. The therapy time to deliver the NCS therapeutic dose of 10000 RBE-cGy, is 27 times longer when 157Gd is used instead of 10B. The skin dose to the treated joint is 33 times larger when 157Gd is used instead of 10B. Furthermore, the impact of using 157Gd instead of 10B was examined in terms of shielded whole-body dose to the patient. The effective dose is 202 mSv for GNCS, compared to 7.6 mSv for BNCS. This is shown to be a result of the longer treatment times required for GNCS; the contribution of the high-energy photons emitted from neutron capture in gadolinium is minimal. Possible explanations as to the relative performance of 157Gd and 10B are discussed, including differences in the RBE and range of boron and gadolinium neutron capture reaction products, and the relative values of the 10B and 157Gd thermal neutron capture cross section as a function of neutron energy.
Regenerative Adsorption and Removal of H <sub>2</sub> S from Hot Fuel Gas Streams by Rare Earth Oxides (2006)

Sorbent materials that allow for high-temperature, regenerative desulfurization of fuel gas streams for the anode of a solid oxide fuel cell have been developed. Reversible adsorption of H2S on cerium and lanthanum oxide surfaces is demonstrated over many cycles at temperatures as high as 800 degrees C, on both fresh or presulfided sorbents, and at very high space velocities. The adsorption and desorption processes are very fast, and removal of H2S to sub-parts per million levels is achieved at very short (millisecond) contact times. Any type of sulfur-free gas, including water vapor, can be used to regenerate the sorbent surface. Preferably, the anode off-gas stream is used to sweep the desorbed H(2)S to a burner.
Superconducting single and phased-array probes for clinical and research MRI (2003)

Significant improvement of the signal-to-noise ratio (SNR) for magnetic resonance imaging (MRI) applications, in which the thermal noise of the rf receiver probe dominates the system noise can be achieved by cooling down a normal metal probe or by using superconductors. In this work, the SNR enhancement expected from using superconductors for single coil and/or phased array designs are calculated, discussed and compared with some experimental results. We also report on the design and fabrication of a 63.8 MHz probe (1.5 Tesla) consisting of patterned, copper or YBCO films deposited on both sides on a 5 cm LaAlO/sub 3/ substrate. The unloaded Q of the normal metal probe at room temperature and at 77 K was about 400 and 1000, respectively, while the YBCO probe exhibited a Q of 40 000 at 77 K. Five-cm diameter probes cooled to 77 K were superior to their identically designed room temperature equivalents, and provided SNR gains at 1.5 Tesla of 3 and 2 times for YBCO and cooled normal metal, respectively. The application of superconducting coils in conjunction with recently developed techniques for significant reduction of MRI acquisition times by using parallel processing with phased array probes is discussed.
Design and evaluation of a photonic FFT processor (2002)

Free-space optical interconnection (FSOI) of integrated circuits (ICs) shows great potential for the efficient implementation of high-performance parallel computing and signal processing systems. The design study of a high-speed FSOI FFT processor system is the objective of this paper. The FFT is an important mathematical operation in many applications such as signal processing, telecommunications, and instrumentation which makes it an excellent choice to incorporate in a high-speed system. The result of this work is the design of a photonic FFT system that includes the Photonic FFT (PFFT) chip and the Photonic RAM (PRAM) chip.
Human Milk Oligosaccharides Modulate Fecal Microbiota and Are Safe for Use in Children With Overweight (2021)

Both 2'FL and the Mix beneficially modulate intestinal microbiota by increasing bifidobacteria. Furthermore, supplementation with either 2'FL alone or a Mix is safe and well tolerated in children.
Development of Dihydroxyphenyl Sulfonylisoindoline Derivatives as Liver-Targeting Pyruvate Dehydrogenase Kinase Inhibitors (2017)

Pyruvate dehydrogenase kinases 1-4 (PDK1-4) negatively control activity of the pyruvate dehydrogenase complex (PDC) and are up-regulated in obesity, diabetes, heart failure, and cancer. We reported earlier two novel pan-PDK inhibitors PS8 [4-((5-hydroxyisoindolin-2-yl)sulfonyl)benzene-1,3-diol] (1) and PS10 [2-((2,4-dihydroxyphenyl)sulfonyl)isoindoline-4,6-diol] (2) that targeted the ATP-binding pocket in PDKs. Here, we developed a new generation of PDK inhibitors by extending the dihydroxyphenyl sulfonylisoindoline scaffold in 1 and 2 to the entrance region of the ATP-binding pocket in PDK2. The lead inhibitor (S)-3-amino-4-(4-((2-((2,4-dihydroxyphenyl)sulfonyl)isoindolin-5-yl)amino)piperidin-1-yl)-4-oxobutanamide (17) shows a ∼8-fold lower IC<sub>50</sub> (58 nM) than 2 (456 nM). In the crystal structure, the asparagine moiety in 17 provides additional interactions with Glu-262 from PDK2. Treatment of diet-induced obese mice with 17 resulted in significant liver-specific augmentation of PDC activity, accompanied by improved glucose tolerance and drastically reduced hepatic steatosis. These findings support 17 as a potential glucose-lowering therapeutic targeting liver for obesity and type 2 diabetes.
A model for classical wavefront sensors and snapshot incoherent wavefront sensing (2019)

A new formula is derived to connect between slopes wavefront sensors (e.g. Shack-Hartmann) and curvature sensors (based on Transport-of-Intensity Equation). Experimental results demonstrate snapshot simultaneous phase and intensity recovery on an incoherent illumination microscopy.
Microlens-Stabilized Laser Arrays for Short-Pulse, Speckle-Free Imaging (2018)

Microlens-stabilized laser arrays were constructed using solid-state and liquid gain media. Arrays comprised of more than 1,200 beams illuminated motile biological specimens, producing speckle-free video with 15 nanosecond exposure per frame.
Constructing Free-Energy Approximations and Generalized Belief Propagation Algorithms (2005)

Important inference problems in statistical physics, computer vision, error-correcting coding theory, and artificial intelligence can all be reformulated as the computation of marginal probabilities on factor graphs. The belief propagation (BP) algorithm is an efficient way to solve these problems that is exact when the factor graph is a tree, but only approximate when the factor graph has cycles. We show that BP fixed points correspond to the stationary points of the Bethe approximation of the free energy for a factor graph. We explain how to obtain region-based free energy approximations that improve the Bethe approximation, and corresponding generalized belief propagation (GBP) algorithms. We emphasize the conditions a free energy approximation must satisfy in order to be a "valid" or "maxent-normal" approximation. We describe the relationship between four different methods that can be used to generate valid approximations: the "Bethe method", the "junction graph method", the "cluster variation method", and the "region graph method". Finally, we explain how to tell whether a region-based approximation, and its corresponding GBP algorithm, is likely to be accurate, and describe empirical results showing that GBP can significantly outperform BP.
Analysis of O6-methylguanine-DNA methyltransferase in individual human cells by quantitative immunofluorescence microscopy. (1994)

A quantitative assay of immunofluorescence is described that can be performed on individual cells from standard pathologic specimens using fluorescence microscopy. The technique has been applied to measurement of O6-methylguanine-DNA methyltransferase, a DNA repair protein that is a molecular marker for resistance to chloroethylnitrosources used in cancer chemotherapy. The immunofluorescence assay makes use of monoclonal antibodies with specificity for human transferase, fluorescence microscopy with digital imaging, fluorescent bead internal standards, and computerized image analysis. This method is specific for the transferase, produces results correlated with activity measurements, and yields new data about tissue heterogeneity and subcellular localization previously unavailable with standard assay methods.
Mobile ranging with low accuracy clocks (2003)

Our position location technique provides location information within milliseconds and is integrated in a handheld, direct sequence spread spectrum (DSSS), communications system. We use a standard DSSS waveform, with a state-of-the-art chipping rate to provide a position location capability to an accuracy of less than one meter in a severe multipath environment. We use a two-way time-of-arrival (TOA) measurement technique (with 1 ppm clocks) that eliminates the need to synchronize master and reference radio clocks. Several techniques improve TOA and therefore range accuracy. A loop back calibrates internal system delay. Frequency diversity orthogonalizes multipath with respect to direct path and leading edge curve fitting of the direct path reduces the effect of multipath. Applications include location of urban warfighters, firefighters, police, and medical personnel and resources.
Identification and utilization of genes associated with beef qualities (2011)

The fatty acid composition of adipose tissue in beef has been recognized as an important trait because of its relationship with beef quality, including favorable beef flavor and tenderness. Over the last decade, we have tried to identify the genes responsible for the fatty acid composition in cattle, and have found the following. (i) Genetic polymorphism of stearoyl-CoA desaturase (SCD) is one of the responsible genes associated with fatty acid composition. The average effects of gene substitution of the SCD type A gene on the monounsaturated fatty acid (MUFA) percentage and the melting point of intramuscular fat were approximately +1.0% and -1.0°C, respectively. (ii) Intron polymorphism of sterol regulatory element binding protein-1 (SREBP-1) also affected MUFA. (iii) No effect of SCD or SREBP-1 genotypes on any representative carcass traits of Japanese Black in the field population was observed. (iv) Additional genetic markers adipocytes fatty acid binding protein 4 (FABP4) and liver X receptor α also affected the fatty acid composition. (v) SCD and FABP4 significantly affected fatty acid composition in Holstein steers. These findings will bring new insight into the fat-related carcass traits of beef cattle and will thus contribute to the beef industry.
MediaPipe Hands: On-device Real-time Hand Tracking (2020)

We present a real-time on-device hand tracking pipeline that predicts hand skeleton from single RGB camera for AR/VR applications. The pipeline consists of two models: 1) a palm detector, 2) a hand landmark model. It's implemented via MediaPipe, a framework for building cross-platform ML solutions. The proposed model and pipeline architecture demonstrates real-time inference speed on mobile GPUs and high prediction quality. MediaPipe Hands is open sourced at https://mediapipe.dev.
Studies on Antifungal Agents (1988)

The in vitro antifungal activity of a series of novel 5,5-disubstituted-3-phenyl-3-(1H-imidazol-1-ylmethyl)-2-methyli soxazolidines and related compounds against Trichophyton rubrum, Aspergillus fumigatus and Candida albicans was studied. The screening was performed in solid agar cultures and some structure-activity relationships involving the C-5-substitution pattern are discussed.
Single-wavelength 108 Gb/s upstream OFDMA-PON transmission (2009)

We experimentally demonstrate 108-Gb/s/λ upstream OFDMA-PON transmission over 20km SSMF and a 1∶32 split, enabled by ONU carrier suppression and OLT heterodyne coherent reception. This is the highest-rate single-wavelength upstream PON transmission to date.
Introducing directly induced microglia-like (iMG) cells from fresh human monocytes: a novel translational research tool for psychiatric disorders (2015)

Microglia, glial cells with immunological functions, have been implicated in various neurological diseases and psychiatric disorders in rodent studies, and human postmortem and PET studies. However, the deeper molecular implications of living human microglia have not been clarified. Here, we introduce a novel translational research approach focusing on human microglia. We have recently developed a new technique for creating induced microglia-like (iMG) cells from human peripheral blood. Two cytokines, GM-CSF and IL-34, converted human monocytes into the iMG cells within 14 days, which show various microglial characterizations; expressing markers, forming a ramified morphology, and phagocytic activity with various cytokine releases. We have already confirmed the applicability of this technique by analyzing iMG cells from a patient of Nasu-Hakola disease (NHD; Ohgidani et al., 2014). We herein show possible applications of the iMG cells in translational research. We believe that this iMG technique will open the door to explore various unknown dynamic aspects of human microglia in psychiatric disorders. This also opens new routes for psychopharmacological approach such as drug efficacy screening and personalized medicine.
Characterization of Water Mist Sprays Using a Phase-Doppler-Particle-Analyzer and an Iso-Kinetic Sampling Probe (2004)

A Phase-Doppler-Particle-Analyzer (PDPA) was used to screen candidate water mist nozzles for use in a scaling validation aimed to allow scaled-down testing of water mist systems. A custom-designed iso-kinetic sampling probe (IKSP) was developed to independently measure water mist fluxes at the same locations where PDPA measurements were made. Measurements were taken at two elevations in selected full-cone water mist sprays. The water drop size was found to increase with radial distance from the spray centerline, while the mean drop velocity and drop concentration decrease with radial distance. Gross drop size distributions of water mist sprays were derived from local drop size distributions and water fluxes measured in two spray cross sections. It was found that, for the water mist sprays investigated in this study, both Rosin-Rammler and log-normal distributions are required to correlate the entire drop size spectrum. In general, the agreement between the mist fluxes measured with the PDPA and iso-kinetic sampling was within 7% near the spray centerline. The selected nozzles show appropriate intended scaling in terms of the drop size, nozzle discharge pressure, and water discharge rate.