gebhart/patrepeval huggingface dataset:
A novel tetravalent formulation combining the four aggregated domain III-capsid proteins from dengue viruses induces a functional immune response in mice and monkeys (2015)

Our group developed a subunit vaccine candidate against dengue virus based on two different viral regions: the domain III of the envelope protein and the capsid protein. The novel chimeric protein from dengue-2 virus [domain III-capsid (DIIIC-2)], when presented as aggregated incorporating oligodeoxynucleotides, induced anti-viral and neutralizing antibodies, a cellular immune response and conferred significant protection to mice and monkeys. The remaining constructs were already obtained and properly characterized. Based on this evidence, this work was aimed at assessing the immune response in mice of the chimeric proteins DIIIC of each serotype, as monovalent and tetravalent formulations. Here, we demonstrated the immunogenicity of each protein in terms of humoral and cell-mediated immunity, without antigen competition on the mixture forming the formulation tetra DIIIC. Accordingly, significant protection was afforded as measured by the limited viral load in the mouse encephalitis model. The assessment of the tetravalent formulation in non-human primates was also conducted. In this animal model, it was demonstrated that the formulation induced neutralizing antibodies and memory cell-mediated immune response with IFN-γ-secreting and cytotoxic capacity, regardless the route of immunization used. Taken together, we can assert that the tetravalent formulation of DIIIC proteins constitutes a promising vaccine candidate against dengue virus, and propose it for further efficacy experiments in monkeys or in the dengue human infection model, as it has been recently proposed.
Toxicity of Linalool to Life Stages of the Cat Flea, Ctenocephalides felis (Siphonaptera: Pulicidae), and its Efficacy in Carpet and on Animals (1988)

The toxicity of linalool to eggs, larvae, pupae, and adults of the cat flea (Ctenocephalides felis) was determined. In vitro, linalool was equally active against eggs and adults; the larvae were least susceptible. Linalool killed eggs, larvae, and pupae in carpet squares; treatment of eggs with a 1.0% emulsion and treatment of larvae or pupae with a 5.0% emulsion prevented all adult emergence. Fleas were eliminated from cats treated with dips of 1.0% linalool and of 0.5% linalool with synergist. Linalool acts rapidly on adult fleas and its activity is synergized by piperonyl butoxide. Because linalool has low vertebrate toxicity and is active against all life stages of the flea, it has major potential for use in control programs for C. felis.
[Selection of a peptide mimic the neutralization epitope of hepatitis E virus with phage peptide display technology]. (2003)

Hepatitis E is an acute hepatitis casused by hepatitis E virus (HEV) in developing countries, where it occurs as cases sporadic and in epidemics form. The causative agent, hepatitis E virus, is transmitted primarily by the fecal-oral route. HEV is icosahedron non-enveloped virus, and its genome is a single-stranded, positive-sense, 3'-polyadenylated RNA about 7.5 kb in length. It contains three open reading frames (ORFs). Of which ORF1 codes for a polyprotein of 1693 amino acids and contain domains homologous to a viral methyltransferase, a papainlike cysteine protease, an RNA helicasre, and an RNA-dependent RNA polymerase, besides the most hypervariable region of the HEV genome. And ORF3 codes for a 123-amino-acide-long polypeptide with unknown function. While the major viral capsid protein (pORF2, ORF2 codes) of 660 amino acid was showed to contain the protective epitope. The bacterially expressed polypeptide disignated as NE2 has been proved to be a protective antige. And the anti-NE2 monoclonal antibodies (mAb) was screend, two of these mAbs 8C11 and 8H3 were showed to be against separate conformational neutralization epitope of hepatitis E virus (HEV). And these two mAb were used to screen for binding peptides from a 7-peptides phage display library. After four rounds of panning, tweenty-one positive monoclonal phages (11 for 8C11, and 10 for 8H3) were selected and the inserted fragments were sequenced. The DNA sequence coding for the obtained dominant peptide 8C11 (N'-His-Pro-Thr-Leu-Leu-Arg-Ile-C', named 8C11A) and 8H3 (N'-Ser-Ile-Leu-Pro-Tyr-Pro-Tyr-C', named 8H3A) were then synthesized and cloned to insert between amino acid 78 to 83 of hepatitis B core antigen (HBcAg), then expressed in E. coli. The recombinant proteins aggregate into homodimer or polymer on SDS-PAGE, and could bind with mAb 8C11 and 8H3 in Western blotting. Respectively, the recombinant protein C8C11A showed to be dimer mainly, which can bind with mAb 8C11. The monomer and dimer of C8H3A are in the same amount on SDS-PAGE, but only the dimer could bind with mAb 8H3 on Western blotting. The renatured recombinant proteins were all showed to aggregate into virus like particles which were similar as HBcAg on transmission electron micrograph. The dominant peptide 8H3A (N'-Ser-Ile-Leu-Pro-Tyr-ProTyr-C') that selected out by mAb 8H3 was further chemo-synthesized, and its binding activity was confirmed by BIAcore biosensor. The result showed that this 7-peptide can bind with mAb 8H3 in a big Ka and Kd form, which means the binding is not stable. These results implicated that conformational dependent neutralization epitope could be partially modeled by short peptide, which provided a feasible route for subunit vaccine development.
Optical distortion correction in Optical Coherence Tomography for quantitative ocular anterior segment by three-dimensional imaging (2010)

A method for three-dimensional 3-D optical distortion\n(refraction) correction on anterior segment Optical Coherence Tomography\n(OCT) images has been developed. The method consists of 3-D ray tracing\nthrough the different surfaces, following denoising, segmentation of the\nsurfaces, Delaunay representation of the surfaces, and application of fan\ndistortion correction. The correction has been applied theoretically to\nrealistic computer eye models, and experimentally to OCT images of: an\nartificial eye with a Polymethyl Methacrylate (PMMA) cornea and an\nintraocular lens (IOL), an enucleated porcine eye, and a human eye in vivo\nobtained from two OCT laboratory set-ups (time domain and spectral). Data\nare analyzed in terms of surface radii of curvature and asphericity.\nComparisons are established between the reference values for the surfaces\n(nominal values in the computer model; non-contact profilometric\nmeasurements for the artificial eye; Scheimpflug imaging for the real eyes\nin vivo and vitro). The results from the OCT data were analyzed following\nthe conventional approach of dividing the optical path by the refractive\nindex, after application of 2-D optical correction, and 3-D optical correction\n(in all cases after fan distortion correction). The application of 3-D optical\ndistortion correction increased significantly both the accuracy of the radius\nof curvature estimates and particularly asphericity of the surfaces, with\nrespect to conventional methods of OCT image analysis. We found that the\ndiscrepancies of the radii of curvature estimates from 3-D optical distortion\ncorrected OCT images are less than 1% with respect to nominal values.\nOptical distortion correction in 3-D is critical for quantitative analysis of\nOCT anterior segment imaging, and allows accurate topography of the\ninternal surfaces of the eye.
Direct gain modulation of a semiconductor laser by a GaAs picosecond optoelectronic switch (1983)

We report a novel application of high-speed optoelectronic switches for direct gain modulation of semiconductor lasers. A GaAs/GaAlAs buried heterostructure laser is driven by a Cr-doped GaAs photoconducting switch activated by a synchronously mode-locked cw dye laser. Infrared light pulses of 55-ps width are emitted from the semiconductor laser.
<i>In vitro</i> activity of novel <i>in silico</i>‐developed antimicrobial peptides against a panel of bacterial pathogens (2013)

Antimicrobial-peptide-based therapies could represent a reliable alternative to overcome antibiotic resistance, as they offer potential advantages such as rapid microbicidal activity and multiple activities against a broad spectrum of bacterial pathogens. Three synthetic antimicrobial peptides (AMPs), AMP72, AMP126, and also AMP2041, designed by using ad hoc screening software developed in house, were synthesized and tested against nine reference strains. The peptides showed a partial β-sheet structure in 10-mM phosphate buffer. Low cytolytic activity towards both human cell lines (epithelial, endothelial, and fibroblast) and sheep erythrocytes was observed for all peptides. The antimicrobial activity was dose dependent with a minimum bactericidal concentration (MBC) ranging from 0.17 to 10.12 μM (0.4-18.5 µg/ml) for Gram-negative and 0.94 to 20.65 μM (1.72-46.5 µg/ml) for Gram-positive bacteria. Interestingly, in high-salt environment, the antibacterial activity was generally maintained for Gram-negative bacteria. All peptides achieved complete bacterial killing in 20 min or less against Gram-negative bacteria. A linear time-dependent membrane permeabilization was observed for the tested peptides at 12.5 µg/ml. In a medium containing Mg²⁺ and Ca²⁺, the peptide combination with EDTA restores the antimicrobial activity particularly for AMP2041. Moreover, in combination with anti-infective agents (quinolones or aminoglycosides) known to bind divalent cation, AMP126 and AMP2041 showed additive activity in comparison with colistin. Our results suggest the following: (i) there is excellent activity against Gram-negative bacteria, (ii) there is low cytolytic activity, (iii) the presence of a chelating agent restores the antimicrobial activity in a medium containing Mg²⁺ and Ca²⁺, and (iv) the MBC value of the combination AMPs-conventional antibiotics was lower than the MBC of single agents alone.
Machine Learning-Based Method for Prediction of Virtual Network Function Resource Demands (2019)

Software-Defined Networking (SDN) and Network Function Virtualization (NFV) are paradigms that help administrators to manage dynamic networks. While SDN allows centralized network control, NFV provides flexible and scalable Virtual Network Functions (VNFs). These paradigms are also enablers for concepts such as Service Function Chaining (SFC) where chains are composed of several VNFs to provide a specific service. However, in order to maximize the benefits from the above-mentioned flexibility, new research questions need to be addressed, e.g., regarding effective management processes for dynamic networks. We proposed a novel learning model based on the flexibility of softwarization and abundant volume of monitoring data in NFV environments to predict VNF resource demands using SFC data. Our model is based on Context and Aspect Embedded Attentive Target Dependent Long Short Term Memory (CAT-LSTM) that consists of Target-Dependent LSTM (TD-LSTM), context embedding, aspect embedding, and attention. We developed this model to obtain high accuracy for the prediction of VNF resources such as the CPU. Our model uses two labeling systems: the qualitative resource state and the quantitative resource usage, both of which are used to evaluate its performance. This assists the administrator in understanding the network conditions, improves prediction performance, and provides practically useful information. Our learning model for predicting VNF resource demands can be utilized to solve essential SFC problems such as auto-scaling and optimal placement, which in turn prevent service interruption and provide high reliability.
Mechanisms of Action of Conjugated Linoleic Acid: Evidence and Speculation (2000)

Abstract. Conjugated linoleic acid (CLA) has been shown to inhibit carcinogenesis and atherosclerosis, enhance immunologic function while protecting against the catabolic effects of immune stimulation, affect body composition change (reducing body fat gain while enhancing lean body mass gain), and stimulate the growth of young rats. We discuss possible biochemical mechanisms that underlie these physiological effects. We emphasize the importance of considering the effects, both individually and combined, of the two CLA isomers ( cis ‐9, trans ‐11 CLA and trans ‐10, cis ‐12 CLA) that have been shown to exhibit biological activity and which appear to exert their effects via different biochemical mechanisms.
Standardization of second-order instruments (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStandardization of second-order instrumentsYongdong. Wang and Bruce R. KowalskiCite this: Anal. Chem. 1993, 65, 9, 1174–1180Publication Date (Print):May 1, 1993Publication History Published online1 May 2002Published inissue 1 May 1993https://pubs.acs.org/doi/10.1021/ac00057a012https://doi.org/10.1021/ac00057a012research-articleACS PublicationsRequest reuse permissionsArticle Views160Altmetric-Citations29LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Relationship of Oxidized Phospholipids on Apolipoprotein B-100 Particles to Race/Ethnicity, Apolipoprotein(a) Isoform Size, and Cardiovascular Risk Factors (2009)

OxPL/apoB levels vary according to race/ethnicity, are largely independent of cardiovascular risk factors, and are inversely associated with apo(a) isoform size. The association of OxPL with small apo(a) isoforms, in which a similar relationship is present among all racial/ethnic subgroups despite differences in Lp(a) levels, may be a key determinant of cardiovascular risk.
Gradient based Textural Characterization of Fingerprints (2008)

Though a lot of research has been done to match fingerprints, most existing approaches rely on locations of minutiae features for matching tasks. Relatively, little effort has gone into utilizing textural information present in fingerprints as distinguishing characteristic. In this paper, we propose a novel gradient-based approach to characterize textural information in fingerprints for the task of biometric matching. In particular, the proposed approach uses histograms of oriented gradients (HOGs) to represent minutiae neighborhoods. The minutiae neighborhoods are divided into several regions to make the computed histograms distinguishing and robust at the same time. Experimental results are provided to show the efficacy of the proposed characterization.
In-vitro activity of purpuromycin and MDL 63, 604 against microorganisms that cause vaginitis and vaginosis (1995)

Purpuromycin and its semi-synthetic derivative MDL 63,604 had in-vitro activity similar to that of amphotericin B against isolates of Candida albicans. MDL 63,604 had activity similar to that of metronidazole against Trichomonas vaginalis. Both compounds were very active against most species of Gram-positive and Gram-negative anaerobes and against Gardnerella vaginalis. MDL 63,604 had significantly lower MICs than purpuromycin against T. vaginalis and most of the bacteria, probably due to antagonism of purpuromycin's activity by medium supplements (blood or serum). Purpuromycin or related compounds may have a potential role in the topical treatment of vaginitis and vaginosis.
ChemInform Abstract: ANTI‐INFLAMMATORY 1‐SUBSTITUTED 2‐IMIDAZOLIDINONES (1978)

Abstract Imidazolidinon (I) wird in Gegenwart von Kaliumcarbonat mit Arylmethylchloriden (II) N‐alkyliert.
Transmissive liquid crystal cell parameters measurement by spectroscopic ellipsometry (2001)

A powerful method for liquid crystal (LC) cell twist angle and retardation measurement is presented. This method is based on the spectroscopic ellipsometry. By varying the polarizer and analyzer angles only, a transmission spectrum containing null transmission at some particular wavelengths can be obtained. Analytical equations derived bases on a polarization analysis of twisted birefringent layers are then solved to give all the LC cell parameters. In general, the twist angle, twist sense, retardation, and the rubbing direction can all be determined. Measurement results on commercial liquid crystal display panels are presented.
Catalyst compositional for hydroprocessing petroleum feedstocks (1992)

This patent describes a catalyst composition useful in the hydroprocessing of a sulfur- and metal-containing hydrocarbon feedstock. It comprises 1.0-5.0 weight percent of an oxide of nickel or cobalt and 10.0-25.0 weight percent of an oxide of molybdenum, all supported on a porous alumina support in such a manner that the molybdenum gradient of the catalyst has a value of less than 6.0, 15-30% of the nickel or cobalt is in an acid extractable form, and the catalyst is further characterized by having a total surface area of 150-210 m{sup 2}/g, a total pore volume of 0.50-0.75 cc/g, and a pore size distribution such that pores having diameters of less than 100A constitute less than 25.0%, pores having diameters of 100-160A constitute 70.0-85.0% and pores having diameters of greater than 250A constitute 1.0-15.0% of the total pore volume of the catalyst.
Incorporation of electrically charged N-alkyl amino acids into ribosomally synthesized peptides via post-translational conversion (2013)

N-Alkyl amino acids are invaluable building blocks for ribosomally synthesized peptides because they can increase proteolytic stability and cell-permeability of peptides. However, previous studies showed that N-alkyl amino acids bearing an electrically charged side-chain cannot be directly incorporated into ribosomally synthesized peptides. Here we report a simple and robust method for ribosomal synthesis of peptides containing charged N-alkyl amino acids. In this method, an N-alkyl amino acid precursor, in which the charge of the translation-incompatible charged N-alkyl amino acid is masked, is ribosomally incorporated into a peptide. Subsequently, the precursor is post-translationally converted into a charged N-alkyl amino acid by bio-orthogonal chemical or enzymatic reaction. Using this method, we demonstrate the efficient incorporation of amine- or carboxylate-containing N-alkyl amino acids into ribosomally synthesized peptides and its compatibility with our recently developed in vitro transcription–translation coupled with association of puromycin-linker (TRAP) display. This study represents an important step toward in vitro display selection from structurally diverse N-alkyl-peptide libraries that will facilitate the discovery of proteolytically stable and cell-permeable peptidomimetics from libraries with high diversity.
Query clustering using content words and user feedback (2001)

Query clustering is crucial for automatically discovering frequently asked queries (FAQs) or most popular topics on a question-answering search engine. Due to the short length of queries, the traditional approaches based on keywords are not suitable for query clustering. This paper describes our attempt to cluster similar queries according to their contents as well as the document click information in the user logs.
On the Weakest Failure Detector for Quiescent Reliable Communication (1997)

We consider the problem of achieving reliable communication with quiescent algorithms (i.e., algorithms that eventually stop sending messages) in asynchronous systems with process crashes and lossy links, and show that, among failure detectors with bounded output size, \\SigmaP is the weakest one that can be used to solve this problem. Combined with a result in [ACT97], this shows that failure detectors that are commonly used in practice, i.e., those that output lists of suspects, are not always the best ones to solve a problem. 1 Introduction In [ACT97], we considered the problem of achieving reliable communication with quiescent algorithms (i.e., algorithms that eventually stop sending messages) in asynchronous systems with process crashes and lossy links. In that paper, we show that this problem can be solved with heartbeat, a failure detector that can be implemented in such systems (without timeouts). Unlike failure detectors that are commonly considered in the literature and used...
Developing integrated fuzzy guidance law for aerodynamic homing missiles by genetic algorithm (2009)

Fuzzy logic controller (FLC) is well-known of its robustness to parameter variations and ability to reject noise. But its design requires defining many parameters. In this work, a systematic and simple procedure is proposed to develop an integrated fuzzy based guidance law which consists of three FLC. Each one of them is activated in a region of the interception. To allow smooth transition between these controllers, another fuzzy-based switching system is introduced. The parameters of all the fuzzy controllers which include the distribution of the membership functions and the rules are simply obtained by observing the function of each controller. Furthermore, these parameters are optimally tuned by the method of genetic algorithms through solving an optimization problem to minimize the interception time, the missile acceleration commands and the miss distance. The simulation results show that the proposed procedure was able to generate a guidance law with a satisfactory performance.
Effects of Segmented Electrode in Hall Current Plasma Thrusters (2001)

Segmented electrodes with a low secondary electron emission are shown to alter significantly plasma flow in the ceramic channel of the Hall thruster. The location of the axial acceleration region relative to the magnetic field can be moved. The radial potential distribution can also be altered near the channel walls. A hydrodynamic model shows that these effects are consistent with a lower secondary electron emission of the segmented electrode as compared to ceramic channel walls.