gebhart/patrepeval huggingface dataset:
The peptide synthetase gene phsA from Streptomyces viridochromogenes is not juxtaposed with other genes involved in nonribosomal biosynthesis of peptides (1996)

By complementation of a previously described non-phosphinothricin tripeptide (PTT)-producing mutant, NTG1, which is blocked in nonribosomal synthesis of the peptide, a DNA fragment including the putative peptide synthetase gene phsA was isolated (W. Wohlleben, R. Alijah, J. Dorendorf, D. Hillemann, B. Nussbaumer, and S. Pelzer, Gene 115:127-132, 1992). Sequence analysis of phsA revealed that it encodes a protein of 622 amino acids with regions which are highly similar to core motifs characteristic for peptide synthetases. PhsA represents one functional domain of a peptide synthetase which is necessary for activation and condensation of one amino acid, probably N-acetyl-demethyl-phosphinothricin. With regard to the arrangement of the flanking genes, phsA is the first peptide synthetase gene which is not in the direct neighborhood of additional peptide synthetase genes involved in the formation of peptide antibiotics. Gene disruption mutants with internal fragments of phsA subcloned in temperature-sensitive pGM vectors were generated. Integration occurred either into the chromosomal copy of phsA or into a gene outside the known phsA locus, resulting in two classes of non-PTT-producing mutants. In cofeeding experiments the former phsA mutants showed the same phenotype as did NTG1, which confirmed participation of phsA in nonribosomal synthesis of PTT. A truncated phsA gene was overexpressed in Escherichia coli, and the resulting protein of 593 amino acids was purified for raising antibodies. By performing immunoblotting experiments, the expression of phsA could be detected in Streptomyces viridochromogenes Tü494 in the stationary-growth phase after 4 days of incubation.
Multi-Voltage SOI-BiCDMOS for 14V&42V Automotive Applications (2004)

This paper presents a new multi-voltage SOI-BiCDMOS, which particularly focuses on “Power MOSFETs and BJTs rich automotive applications”. This technology can integrate Nch LDMOSs and Pch LDMOSs which have 35V/6OV/8OV breakdown voltages, high packing density deep trench isolated BJTs, and a low cost 0.8~ CMOS, on a single chip. The six types of LDMOSs can be simultaneously fabricated with only two additional masks to a CMOS process, and these LDMOSs satisfy both low specific on-resistance and good SOA. Furthermore, in this technology a bonded SO1 wafer with 200mm diameter has been newly adopted in order to reduce chip cost.
Secure Network Objects (1999)

We describe the design and implementation of secure network objects, which provide security for object-oriented network communication. The design takes advantage of objects and subtyping to present a simple but expressive programming interface for security, supporting both access control lists and capabilities. The implementation of this design fits nicely within the structure of the existing network objects system; we discuss its internal components, its performance, and its use in some applications.
Atomic Scale Compositional and Structural Characterization of Nanostructured Materials Using Combined FIB, STEM, and LEAP (2006)

Extended abstract of a paper presented at Microscopy and Microanalysis 2006 in Chicago, Illinois, USA, July 30 – August 3, 2005
LIVER TRANSPLANTATION FOR METASTATIC NEUROENDOCRINE CARCINOMA (1998)

Liver transplantation may be justified in selected patients to provide immediate relief of otherwise intractable pain or hormone-related symptoms. Transplantation with curative intent appears worthwhile in young patients with only hepatic disease. In older patients with extrahepatic disease requiring extended operations, long-term results are discouraging, and the small benefit achieved by liver transplantation must be weighed against medical treatment options and the natural course of often slowly progressing disease.
Towards efficient semantic real time mapping of man-made environments using Microsoft's Kinect (2011)

We propose a novel approach for efficient semantic mapping of Manhattan-like structure i.e. the frequently observed dominance of three mutually orthogonal vanishing directions in man-made environments. First, we estimate the Manhattan-like structure by using an MSAC variant that estimates the Manhattan system directly from the 3D data. In contrast to other methods we use only the normal vectors of each voxel rather than estimating it indirectly using plane estimation. In a next step we estimate the translative motion of the robot relative to the Manhattan system using constrained visual odometry. The mapping is done using a geometric constrained ICP using a-priori knowledge on the estimated Manhattan system. The ICP registers only points within the same geometry to each other. We show that the geometric constrained ICP generate maps with a significant smaller angular drift than an unconstrained one. Octrees are used for map representation in combination with kd-Trees for ICP. We show the robustness of our Manhattan-estimation using real world data. In this paper we demonstrate our approach using a Microsoft Kinect, while the approach will work with all kind of 2.5D sensors.
Yiddish as a Literary Language (1993)

Abstract Although the word language is always loosely connected with speech, literature is an entirely different form. It relies more heavily on style and technique, and is more artful and fluid in structure. Again, European and Western influences are mentioned; however, strong traditions such as taysh have held rigid standards among Yiddish authors. With the fiercely growing and developing world, the need for change could not be contained, and the age of modern literature began. This chapter describes and enumerates the various elements that have shaped Yiddish literature as it is today. Religious movements and the times of oppression for the Jewish people are some of the triggers that provoked this change. Clearly, literature adapts and molds into the needs and experiences of the people.
Effect of TLR Agonists on the Differentiation and Function of Human Monocytic Myeloid-Derived Suppressor Cells (2015)

Tumors persist by occupying immunosuppressive microenvironments that inhibit the activity of tumoricidal T and NK cells. Monocytic myeloid-derived suppressor cells (mMDSC) are an important component of this immunosuppressive milieu. We find that the suppressive activity of mMDSC isolated from cancer patients can be reversed by treatment with TLR7/8 agonists, which induce human mMDSC to differentiate into tumoricidal M1-like macrophages. In contrast, agonists targeting TLR1/2 cause mMDSC to mature into immunosuppressive M2-like macrophages. These two populations of macrophage are phenotypically and functionally discrete and differ in gene expression profile. The ability of TLR7/8 agonists to reverse mMDSC-mediated immune suppression suggests that they might be useful adjuncts for tumor immunotherapy.
Assay based on electrical impedance spectroscopy to discriminate between normal and cancerous mammalian cells (2018)

In this work we present an assay to discriminate between normal and cancerous cells. The method is based on the measurement of electrical impedance spectra of in vitro cell cultures. We developed a protocol consisting on four consecutive measurement phases, each of them designed to obtain different information about the cell cultures. Through the analysis of the measured data, 26 characteristic features were obtained for both cell types. From the complete set of features, we selected the most relevant in terms of their discriminant capacity by means of conventional statistical tests. A linear discriminant analysis was then carried out on the selected features, allowing the classification of the samples in normal or cancerous with 4.5% of false positives and no false negatives.
High resolution 1H n.m.r. studies of vertebrate blood and plasma (1983)

Spin echo Fourier transform proton n.m.r. spectra of whole blood contain resonances from both erythrocytes and plasma. A large number of well-resolved signals from mobile protons of low-molecular-weight metabolites in plasma and serum have been identified. Spectra from the plasmas of eight animal species and commercial, quality control sera are compared. CaEDTA2- and MgEDTA2- resonances can be used for the simultaneous determination of EDTA-chelatable calcium and magnesium concentrations in intact plasma and other biological fluids. Cholesterol is too immobile to contribute to the spectra of intact plasma, but is readily estimated by n.m.r. in both its free and esterified forms after extraction into methanol.
Alignment and wall control of ultra long carbon nanotubes in water assisted chemical vapour deposition (2008)

This study examined the correlation between the thickness of the iron catalyst film deposited by e-beam evaporation and carbon nanotube (CNT) growth by water-assisted chemical vapour deposition. The number of walls and the alignment of the CNTs in the CNT forest could be controlled by the iron thickness. High resolution transmission electron microscopy was used to examine the catalyst size distribution and individual CNT structure. Atomic force microscopy was used to analyse the surface roughness. The correlation between the surface roughness, catalyst agglomeration, number of walls in the CNT and the alignment of the CNTs in the CNT forest was established. The CNTs grown on an iron catalyst ranging in thickness from 0.1 to 0.5 nm were well aligned with 2–4 walls. On the other hand, the CNTs grown on an iron catalyst ranging in thickness from 0.6 to 1 nm were less well aligned with 4–10 walls. The CNTs grown on an iron catalyst >1 nm in thickness were misaligned with 8–16 walls. The significance of these experimental trends is discussed within the framework of the clusters aggregation and Van der Waals interaction.
Detecting malicious PDF documents (2011)

ENGELSK: As the internet has become the new playground for organized crime and foreign intelligence,\nthe sophistication of internet attacks has increased. The traditional attacks targeting listening\nservices on the target computer is no longer as viable as it used to, much thanks to firewalls,\nNAT and more secure administration of servers. This has forced the attackers to find new targets,\nwhich they have found in client applications, and in the users themselves. Client-side attacks\nare now the most used method of attack on the internet. A popular vector for conducting such\nattacks are malicious PDF documents. Traditional signature based network intrusion detection\nsystems (IDS) have a hard time detecting such threats, and no good alternative solutions have\nbeen discovered.\nIn this thesis we seek the answer to the question ”How can malicious PDF-documents transferred\nin a network be detected? “ An anomaly based network IDS approach was chosen, several\nmachine learning classifiers were investigated and Support Vector Machines gave the best\naccuracy and performance. Several features of PDFs are analyzed in order to retrieve those significant\nfor the detection of malicious PDF documents. Experiments were performed to find the\nbest combination of features and SVM configurations to maximize performance of the detection\nalgorithm. A real world study was also performed by implementing the algorithm in a network\nbelonging to the Norwegian Defence.
Activation of transglutaminase at calcium levels consistent with a role for this enzyme as a calcium receptor protein (1985)

The sensitivity of tissue transglutaminase to activation by Ca2+ and other cellular factors was investigated using the enzyme purified from rat liver. The inclusion of Mg2+ in the assay system appeared to reduce the Ca2+-requirement of the enzyme when native N,N'-dimethylcasein was used as the protein acceptor substrate. However, when this protein was dephosphorylated, the Ca2+-requirement was unaffected by Mg2+. In addition, using this modified assay, a Km for Ca2+ was calculated to be in the range of 3-4 microM, at least an order of magnitude lower than that obtained with native acceptor substrate. Membrane phospholipids, 1,2-diolein and calmodulin were found not to affect the activation of transglutaminase by Ca2+. The sensitivity of transglutaminase to Ca2+ which we have now demonstrated suggests that this enzyme may directly act as a receptor protein for Ca2+ during stimulus-response coupling mediated by this cation.
Studies of the Human c- <i>myb</i> Gene and Its Product in Human Acute Leukemias (1986)

The myb gene is the transforming oncogene of the avian myeloblastosis virus (AMV); its normal cellular homolog, c-myb, is conserved across a broad span of evolution. In humans, c-myb is expressed in malignant hematopoietic cell lines and in primary hematopoietic tumors. Partial complementary DNA clones were generated from blast cells of patients with acute myelogenous leukemia. The sequences of the clones were compared to the c-myb of other species, as well as the v-myb of AMV. In addition, the carboxyl terminal region of human c-myb was placed in an expression vector to obtain protein for the generation of antiserum, which was used to identify the human c-myb gene product. Like v-myb, this protein was found within the nucleus of leukemic cells where it was associated with the nuclear matrix. These studies provide further evidence that c-myb might be involved in human leukemia.
Asymmetric Ring Opening of Meso Epoxides with Thiols:  Enantiomeric Enrichment Using a Bifunctional Nucleophile (1998)

ADVERTISEMENT RETURN TO ISSUEPREVNoteNEXTAsymmetric Ring Opening of Meso Epoxides with Thiols: Enantiomeric Enrichment Using a Bifunctional NucleophileMichael H. Wu and Eric N. JacobsenView Author Information Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138 Cite this: J. Org. Chem. 1998, 63, 15, 5252–5254Publication Date (Web):June 25, 1998Publication History Received28 January 1998Published online25 June 1998Published inissue 1 July 1998https://pubs.acs.org/doi/10.1021/jo980155dhttps://doi.org/10.1021/jo980155dbrief-reportACS PublicationsCopyright © 1998 American Chemical SocietyRequest reuse permissionsArticle Views1537Altmetric-Citations101LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Catalysts,Ethers,Oxides,Substitution reactions,Thiols Get e-Alerts
Hydrocarbon Associated Toxicities: a Case Series and Review of Literature (2020)

Background: Hydrocarbon associated toxicity (HAT) is an emerging threat related to wide scale industrialization and easy access to hydrocarbon-containing chemical compounds. Hydrocarbons have a unique toxicological profile and the principles of managing related toxidromes are considerably different from other toxins. Case reports: Here, we present a case series and in-depth review of the existing literature to show the risks associated with these seemingly harmless chemicals, and the approved guidelines for treating exposed patients. In all three cases, the hydrocarbon was a diluent for a pesticide. The amount of pesticide ingested was nontoxic, while the hydrocarbons caused a dose-independent physical toxicity to the lungs. Discussion: Hydrocarbon associated toxicities often go unnoticed because of their usage as diluents for various other toxic chemicals. Their treatment usually differs from other toxins that may have been consumed with them, albeit in insignificant quantities. Conclusion: Recognition of a hydrocarbon diluent in a consumed toxin marks the first step in the correct treatment. Correct labeling of chemical solutions containing hydrocarbons would go a long way in identifying these toxins.
Chitosan-Coated Silica as a Solid Phase for RNA Purification in a Microfluidic Device (2009)

Chitosan-coated silica particles and chitosan-coated microchannels have been explored as an alternative to a standard silica phase for DNA extraction in a microdevice (Cao, W.; Easley, C. J.; Ferrance, J. P.; Landers, J. P. Anal. Chem. 2006, 78 (20), 7222-7228). A method that exploits the use of aqueous buffers for nucleic acid binding to and release from a solid phase is advantageous, avoiding the reagents used for conventional extraction (isopropanol and guanadinium hydrochloride), which are potent PCR inhibitors. The pH-controlled approach, which promotes nucleic acid binding to and release to the chitosan phase based on a change in buffer pH, is exploited here for RNA purification in a microfluidic device. The chitosan phase reproducibly allowed for higher RNA extraction efficiencies under aqueous conditions (71%) compared to that with a silica phase under chaotropic conditions (53%). The effectiveness of the chitosan phase was demonstrated with the successful purification of RNA from the alveolar rhabdomyosarcoma (ARMS) cancer cell line, with 3.5-fold greater extraction efficiencies than obtained when the same sample was purified using a silica phase: the resulting RNA was found to be amplifiable in reverse-transcription PCR. Low-molecular weight chitosan is also a proven inhibitor of RNases, further demonstrating the advantages of chitosan as a solid phase for RNA purification compared to silica. The chitosan phase is, therefore, a superior choice for extraction and purification of RNA in a microfluidic device and is compatible with biological samples found in a clinical or forensic setting.
Computational design and characterization of a temperature-sensitive plasmid replicon for gram positive thermophiles (2012)

The plasmid described in this work could be useful in future efforts to genetically engineer C. thermocellum, and the method used to generate this plasmid may be useful for others trying to make Ts plasmids.
HEVC real-time decoding (2013)

The new High Efficiency Video Coding Standard (HEVC) was finalized in January 2013. Compared to its predecessor H.264 / MPEG4-AVC, this new international standard is able to reduce the bitrate by 50% for the same subjective video quality. This paper investigates decoder optimizations that are needed to achieve HEVC real-time software decoding on a mobile processor. It is shown that HEVC real-time decoding up to high definition video is feasible using instruction extensions of the processor while decoding 4K ultra high definition video in real-time requires additional parallel processing. For parallel processing, a picture-level parallel approach has been chosen because it is generic and does not require bitstreams with special indication.
Excellent rear side passivation on multi-crystalline silicon solar cells with 20 nm uncapped Al<inf>2</inf>O<inf>3</inf> layer: Industrialization of ALD for solar cell applications (2010)

Current bottlenecks for industrialization of Al <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> O <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> deposited by Atomic Layer Deposition (ALD) for crystalline silicon solar cell applications are low growth rate and stability of thin and uncapped layers during co-firing. First results on the performance of a high throughput ALD proto-type, the Levitrack, are presented. Excellent passivation properties have been obtained after firing, for 12 nm thick films deposited on p-Cz (2.3 Ω.cm) with S <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">eff</sub> <; 15cm/s (Δn=3×10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">15</sup> cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-3</sup> ). These layers are compatible with solar cells that operate at a maximum open-circuit voltage of 720mV. Furthermore, we report on the passivation of 20nm uncapped aluminum oxide layers on the rear of p-type mc-Si bifacial cells. LBIC measurements unveiled excellent passivation properties on areas covered by 20nm of Al <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> O <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> characterized by an IQE of 91% at 980nm. Remarkably, these lifetime and cell results were obtained without lengthy post-treatments like forming gas anneal.
CMOS RF design-the low power dimension (2002)

In many wireless applications power consumption of an RF-IC is more important than integration level due to battery life time considerations. This has been a weak point for CMOS, which has prevented its general acceptance for demanding applications such as cellular and paging. Growing attention is now being paid to low power design of CMOS RF ICs. This paper addresses issues such as technology requirement, transceiver architecture, circuit topologies as well as the extent of integration, in the context of power consumption.
Seaweed Community of the Subtidal Rocky Habitats along the Coast of Geumo Archipelago in the Central South Sea of Korea (2004)

Seaweed community of the sub tidal rocky habitats along the coast of Geumo Archipelago in the central South Sea of Korea is described. This area is characterized by archipelago in which islets are separated by shallow bottom sediments (primary, muddy sand), and turbidity is generally high due to the resuspension of bottom sediments. The hard substrata available for algal attachment are limited to less than 10 m in depth. Thirty sites were randomly chosen along the coast from August 2003 to September 2003 and a 50 m long transect was established at each site. The transect began at a depth of 1 m and ended at the depth of 9 m. The percent cover of all species other than crustose coralline algae was estimated at 2 m depth intervals along the transect using a 0.25 m PVC quadrat with 25 squares. Thirty-six species were identified including 6 Chlorophyta, 10 Phaeophyta and 20 Rhodophyta. Species with more than 5% mean bottom cover were Gelidium amansii, Corallina pilulifera, Amphiroa dilatata and Carpopeltis cornea, which formed dense turf-forming algal assemblages at 1-5 m depth. At all sites except S11-S15 located in the western coast of Sorido, bottom covers of seaweed species at the depth deeper than 7 m were less than 6%. The lower limit of algal assemblages was 9 m in depth. We speculate that the limited water clarity and vertical extent of hard substrata available for the settlement of seaweed species are the direct cause of reducing the diversity, abundance and distributional extent of algal assemblages in the area.