gebhart/patrepeval huggingface dataset:
Predicting Postoperative Liver Dysfunction Based on Blood‐Derived MicroRNA Signatures (2019)

There is an urgent need for an easily assessable preoperative test to predict postoperative liver function recovery and thereby determine the optimal time point of liver resection, specifically as current markers are often expensive, time consuming, and invasive. Emerging evidence suggests that microRNA (miRNA) signatures represent potent diagnostic, prognostic, and treatment-response biomarkers for several diseases. Using next-generation sequencing as an unbiased systematic approach, 554 miRNAs were detected in preoperative plasma of 21 patients suffering from postoperative liver dysfunction (LD) after liver resection and 27 matched controls. Subsequently, we identified a miRNA signature-consisting of miRNAs 151a-5p, 192-5p, and 122-5p-that highly correlated with patients developing postoperative LD after liver resection. The predictive potential for postoperative LD was subsequently confirmed using real-time PCR in an independent validation cohort of 98 patients. Ultimately, a regression model of the two miRNA ratios 151a-5p to 192-5p and 122-5p to 151a-5p was found to reliably predict postoperative LD, severe morbidity, prolonged intensive care unit and hospital stays, and even mortality before an operation with a remarkable accuracy, thereby outperforming established markers of postoperative LD. Ultimately, we documented that miRNA ratios closely followed liver function recovery after partial hepatectomy. Conclusion: Our data demonstrate the clinical utility of an miRNA-based biomarker to support the selection of patients undergoing partial hepatectomy. The dynamical changes during liver function recovery indicate a possible role in individualized patient treatment. Thereby, our data might help to tailor surgical strategies to the specific risk profile of patients.
Novel Dual aromatase-steroid sulfatase inhibitors (2006)

Proc Amer Assoc Cancer Res, Volume 47, 2006

4704 

Aromatase inhibitors (AIs), which inhibit the conversion of androstenedione (Adione) to estrone (E1), are established agents for treating hormone dependent breast cancer (HDBC). It is now recognised that steroid sulfatase (STS) inhibitors, which inhibit the hydrolysis of steroid sulfates to estrogenic steroids (e.g. E1 and androstenediol) will complement AIs in augmenting estrogen deprivation in HDBC. STX64 is the first Phase I trial STS inhibitor. To study the effects of inhibiting aromatase and STS concomitantly by one agent, we recently developed dual aromatase-sulfatase inhibitors (DASIs) by incorporating the STS inhibitory pharmacophore, i.e. a phenol sulfamate ester, into AIs, YM511 (e.g. 1) and Letrozole (e.g. 2). We report here a new class of DASIs which, like 1 and 2, contain the essential features required for potent dual inhibition. Upon evaluation of the compounds in the series in intact JEG-3 cells, using [1β-3H]Adione or [3H]E1 sulfate as substrate, they showed IC50 values against aromatase between 3 and 100 nM, and inhibition of STS activity @ 10 μM between 10μM (c.f. Letrozole: 0.89 nM, STX64: 3.3nM), reduced the plasma estradiol level and inhibited liver STS activity in PMSG treated female Wistar rats both by 75% 3 h after a single 10 mg/kg oral dose. The encouraging in vivo STS inhibition observed for this agent was investigated due to its weak potency shown in vitro . One congener of this agent, which has the heme-ligating group removed, showed a modest 39% inhibition of JEG-3 cells STS activity @ 10 μM. However, when this congener was tested in vivo in the same manner as its parent DASI, it inhibited rat liver STS activity by 95%. These results suggest that these new DASIs are potent AIs in vitro and in vivo . Despite the weak STS inhibitory activities observed for some compounds in vitro , one agent has been shown to inhibit STS strongly in vivo . Like DASIs (1) and (2), these structurally novel DASIs further validate the DASI concept and represents a template for further lead optimisation. The development of a potent DASI should have considerable therapeutic potential for the treatment of HDBC.
Quantifying Surface Area of Nanosheet Graphene Oxide Colloid Using a Gas-Phase Electrostatic Approach (2017)

We demonstrate a new, facile gas-phase electrostatic approach to successfully quantify equivalent surface area of graphene oxide (GO) colloid on a number basis. Mobility diameter (d<sub>p,m</sub>)-based distribution and the corresponding equivalent surface area (SA) of GO colloids (i.e., with different lateral aspect ratios) were able to be identified by electrospray-differential mobility analysis (ES-DMA) coupled to a condensation particle counter (CPC) and an aerosol surface area analyzer (ASAA). A correlation of SA ∝ d<sub>p,m</sub><sup>2.0</sup> was established using the ES-DMA-CPC/ASAA, which is consistent with the observation by the 2-dimensional image analysis of size-selected GOs. An ultrafast surface area measurement of GO colloid was achieved via a direct coupling of ES with a combination of ASAA and CPC (i.e., measurement time was 2 min per sample; without size classification). The measured equivalent surface area of GO was ∼202 ± 7 m<sup>2</sup> g<sup>-1</sup>, which is comparable to Brunauer-Emmett-Teller (BET) surface area, ∼240 ± 59 m<sup>2</sup> g<sup>-1</sup>. The gas-phase electrostatic approach proposed in this study has the superior advantages of being fast, requiring no elaborate drying process, and requiring only a very small amount of sample (i.e., <0.01 mg). To the best of our knowledge, this is the first study of using an aerosol-based electrostatic coupling technique to obtain the equivalent surface area of graphene oxide on a number basis with a high precision of measurement.
Lasergaslegieren – ein Verfahren zur Erzeugung verschleißfester Randschichten auf Titanwerkstoffen (1997)

Abstract Titanlegierungen besitzen hohe spezifische Festigkeiten, sind korrosionsbeständig und haben eine gute Biokompatibilität. Daraus resultiert ihr Einsatz im Bereich Luft‐ und Raumfahrt, der chemischen Industrie, der Medizin und zunehmend auch im Straßenfahrzeugbau. Ein Nachteil der Titanlegierungen ist ihr geringer Widerstand gegen Abrasiv‐ und Gleitverschleiß. Durch Erhöhung der Verschleißfestigkeit können den Titanlegierungen neue Anwendungsfelder erschlossen werden. Mit der im Fraunhofer IWS Dresden entwickelten Methode des Lasergaslegierens gelingt es, sehr tragfähige und verschleißfeste Randschichten auf Titanlegierungen in einem technologisch sicheren Prozeß zu erzeugen. Durch eine zusätzlich mit dem Laser‐Arc‐Verfahren aufgebrachte harte amorphe Kohlenstoffschicht lassen sich die Gleitverschleißeigenschaften noch einmal erheblich verbessern. Nach einer kurzen Vorstellungen der neuen Methoden des Lasergaslegierens werden die enormen Verbesserungen des Verschleßverhaltens anhand von Ergebnissen verschiedener Verschleißversuche nachgewiesen.
Handling pure camera rotation in keyframe-based SLAM (2013)

Handling degenerate rotation-only camera motion is a challenge for keyframe-based simultaneous localization and mapping with six degrees of freedom. Existing systems usually filter corresponding keyframe candidates, resulting in mapping starvation and tracking failure. We propose to employ these otherwise discarded keyframes to build up local panorama maps registered in the 3D map. Thus, the system is able to maintain tracking during rotational camera motions. Additionally, we seek to actively associate panoramic and 3D map data for improved 3D mapping through the triangulation of more new 3D map features. We demonstrate the efficacy of our approach in several evaluations that show how the combined system handles rotation only camera motion while creating larger and denser maps compared to a standard SLAM system.
Design and preclinical characterization of ALXN1210: A novel anti-C5 antibody with extended duration of action (2018)

Eculizumab, a monoclonal antibody (mAb) directed against complement protein C5, is considered to be the current standard of care for patients with paroxysmal nocturnal hemoglobinuria (PNH) and atypical hemolytic uremic syndrome. This study describes the generation and preclinical attributes of ALXN1210, a new long-acting anti-C5 mAb, obtained through select modifications to eculizumab to both largely abolish target-mediated drug disposition (TMDD) and increase recycling efficiency via the neonatal Fc receptor (FcRn). To attenuate the effect of TMDD on plasma terminal half-life (t1/2), histidine substitutions were engineered into the complementarity-determining regions of eculizumab to enhance the dissociation rate of the mAb:C5 complex in the acidic early endosome relative to the slightly basic pH of blood. Antibody variants with optimal pH-dependent binding to C5 exhibited little to no TMDD in mice in the presence of human C5. To further enhance the efficiency of FcRn-mediated recycling of the antibody, two additional substitutions were introduced to increase affinity for human FcRn. These substitutions yielded an additional doubling of the t½ of surrogate anti-mouse C5 antibodies with reduced TMDD in transgenic mice expressing the human FcRn. In conclusion, ALXN1210 is a promising new therapeutic candidate currently in clinical development for treatment of patients with PNH and atypical hemolytic uremic syndrome.
Characterization of recombinant and plant‐derived mistletoe lectin and their B‐chains (1999)

Mistletoe lectin I (pML) and its isoforms ML II and III constitute the active principle in extract preparations from mistletoe, commonly used as immunomodulator in adjuvant tumour therapy. The heterodimeric disulfide-linked cytotoxic protein is classified as type II ribosome inactivating protein (RIP). Recently, the sequence coding for the mistletoe lectin prepro-protein was identified and the existence of a single intron-free gene was shown [Eck, J., Langer, M., Möckel, B., Baur, A., Rothe, M., Zinke, H. & Lentzen, H. (1999) Eur. J. Biochem. 264, 775-784]. The aim of this study was to prepare pure and homogeneous rMLB-chain as well as rML heterodimer for studying the carbohydrate binding specificity of recombinant versus natural protein and its contribution to the observed cytotoxic effect. Expression in E. coli resulted in the production of insoluble protein (inclusion bodies). A procedure for generating correctly folded, biochemically and biologically active rMLB was established starting from the insoluble single chain. Carbohydrate binding and specificity of pMLB and rMLB were analysed by a competitive enzyme linked lectin assay (ELLA). Asialofetuin was able to compete with binding of both chains (50% at 0.8 microM). The specificity of the B-chains to lactose was more distinct with halfmaximal competition at 4.9 mM (pMLB) and > 90 mM (rMLB), respectively. Furthermore, in a coassociation process rMLA- and rMLB inclusion bodies were associated in one step by defined dilution yielding active rML-heterodimer. The activities of recombinant (rML) and plant derived mistletoe lectin (pML) were compared. Cytotoxicity was determined using MOLT-4 cells and enzymatic rRNA N-glycosidase activity was measured in a coupled transcription/translation assay. The IC50 values of the two heterodimers were similar in both assays; rMLB-chain did not show any cytotoxic effect. In the ELLA with lactose as a competitor 50% competition of binding to asialofetuin was achieved at 1.6 mM (rML) and 1.8 mM (pML). Hence, using three different assays we found no significant differences between the recombinant protein and the glycosylated form of ML. Comparing the biological activities of the single chains with those of the heterodimer we conclude, that both, lectin activity and the rRNA N-glycosidase activity, are prerequisites for the cytotoxic effects on target cells.
Multivariable Artificial Pancreas for Various Exercise Types and Intensities (2018)

A consistent GC decline occurred in exercise and recovery periods, which differed with exercise type. The average GC at the start of exercise was above target (185.5 ± 56.6 mg/dL for MCT, 166.9 ± 61.9 mg/dL for resistance training, and 171.7 ± 41.4 mg/dL HIIT), making a small decrease desirable. Hypoglycemic events occurred in 14.6% of exercise sessions and represented only 2.22% of the exercise and recovery period.
Phrase Reordering Model Integrating Syntactic Knowledge for SMT (2007)

Reordering model is important for the sta-tistical machine translation (SMT). Current phrase-based SMT technologies are good at capturing local reordering but not global reordering. This paper introduces syntactic knowledge to improve global reordering capability of SMT system. Syntactic know-ledge such as boundary words, POS infor-mation and dependencies is used to guide phrase reordering. Not only constraints in syntax tree are proposed to avoid the reor-dering errors, but also the modification of syntax tree is made to strengthen the capa-bility of capturing phrase reordering. Fur-thermore, the combination of parse trees can compensate for the reordering errors caused by single parse tree. Finally, expe-rimental results show that the performance of our system is superior to that of the state-of-the-art phrase-based SMT system. 1
Impact of in-plane anisotropic strain on the polarization behavior of vertical-cavity surface-emitting lasers (2000)

We experimentally demonstrate that the presence of switching between two fundamental modes with orthogonal linear polarization in vertical-cavity surface-emitting lasers and the current at which this phenomenon occurs depend on both the magnitude and the orientation of an externally induced in-plane anisotropic strain. We interpret this behavior by considering the anisotropy in gain and in refractive index, both induced by the in-plane strain, and by accounting for a redshift of the two gain curves as a result of current-induced heating.
Regiocontrolled Synthesis of the Trimeric Quinone Framework of Conocurvone (2002)

[reaction: see text] The trimeric quinone framework of conocurvone is crucial for its potent anti-HIV activity. A new synthesis of trimeric quinones based on stepwise substitution of the halogens in 2,3-dihaloquinones by hydroxyquinone anions is described. Chlorinated biquinones are key intermediates that undergo regiospecific substitution reactions to yield trimeric quinone monomethyl ethers.
Calibration and orientation of industrial online photogrammetry systems in situ (2019)

This study proposes an analytical method for calibrating and orienting online photogrammetric systems using a scale bar. The primary idea is building control lengths in the measurement volume by moving and rotating the scale bar and then conducting a self‐calibration bundle adjustment using images of the control lengths. In this study, the five‐point relative orientation method is used to provide estimations of the exterior camera parameters. Additionally, this study elaborates the model of the lengths constrained self‐calibration bundle adjustment, the block computation algorithm and the accuracy assessment. This method obtains both the interior and the exterior orientation parameters of the two cameras, and provides spatial bar length reconstruction precision as the performance evaluation. Simulations are conducted to analyze the calibration accuracy and the results show that the method achieves comparable accuracy and precision against the state‐of‐the‐art method. However, the method does not need steady and well distributed 3D points and is easy to operate. Real data experimental results show that the maximum length measurement error of this method in a volume of 5 m × 4 m × 2 m is <0.4 mm.
Laser assisted dye-sensitized solar cell sealing: From small to large cells areas (2014)

Dye sensitized solar cells (DSCs) modules are an emergent photovoltaic technology but still present some weaknesses in terms of long-term stability: sealing is presently the Achilles' heel of DSCs, which is currently preventing their commercialization. Thermoplastic sealants are normally used to bond the two glass substrates of a DSC. However, these thermoplastic sealants, such as Surlyn®, are permeable towards ambient water vapor, UV sensitive, and they cannot be used above 60 °C. Laser assisted glass-frit sealing is a very interesting alternative sealing technique as it has been demonstrated on microelectromechanical systems assembly. For these applications, the laser is shot around the sealing perimeter several times in a loop pattern. Nevertheless, for sealing large areas without heating the samples, this method cannot be applied since the laser loop takes more than 1 s, which is too long for an effective low-temperature glass-frit sealing. At present, work is described a laser assisted sealing technique that is independent of the sealing border format. This technique is applied to several cells and modules of different shapes and sizes. The technique is suitable for DSCs manufacture and can be implemented in an inline production plant. For the same cell format, efficiencies of the glass-sealed and conventional sealing were compared. Stability studies were also presented to evidence the effectiveness of the hermetic sealing obtained with the newly developed sealing process of DSCs devices.
A Scalable <italic>N</italic>-Color LED Driver Using Single Inductor Multiple Current Output Topology (2015)

Constant current sources are required to drive multiple color LED strings and the LED drivers that drive these LED lighting systems should provide multiple constant currents from a single dc source. In this paper, a single inductor multiple current output (SIMCO) converter that can provide N- independently controllable constant current outputs is reported. The average current in each color LED string is controlled independently by using hybrid average current mode control (HACMC). The HACMC combines the advantage of both average current mode control and charge control method. Traditional dimming approaches such as pulse width modulation and analog dimming can be incorporated in the proposed topology for color tunability and correlated color temperature control. The proposed approach uses a simple control logic, which is modular and can be scalable to N color LED lighting systems. The operation of the SIMCO converter with the HACMC is explained in detail. Simulation and experimental results with 48 V input and 12 W for N = 3 (RGB) color systems are presented.
Surface Acoustic Wave Accelerometer (1981)

L'invention se rapporte aux accelerometres comportant un corps elastique vibrant associe a des moyens electriques permettant d'entretenir des oscillations et d'en mesurer la frequence. The invention relates to accelerometers with a vibrating elastic body associated with electrical means to maintain oscillation and measure the frequency. L'objet de l'invention est un accelerometre comprenant au moins une lamelle elastique (2a et 2b) propageant des ondes elastiques de surface a laquelle une force normale (F) est appliquee resultant de l'action de l'acceleration a mesurer () sur un corps solide ou fluide (50), de masse predeterminee. The object of the invention is an accelerometer comprising at least one elastic lamella (2a and 2b) propagating surface elastic waves on which a normal force (F) is applied resulting from the action of the acceleration to be measured () on a solid or fluid body (50) of predetermined mass. La mesure de la deviation de frequence de l'oscillateur dont fait partie la lamelle donne une indication sur la valeur de la composante normale a l'acceleration. Measuring the oscillator frequency deviation which includes the lamella provides an indication of the value of the normal component to the acceleration. L'accelerometre selon l'invention peut etre utilise a bord d'un vehicule: automobile, avion ou missile. The accelerometer according to the invention can be used in a vehicle: car, plane or missile.
Mucoricin is a Ricin-Like Toxin that is Critical for the Pathogenesis of Mucormycosis (2021)

Abstract Fungi of the order Mucorales cause mucormycosis, a lethal infection with an incompletely understood pathogenesis. We now demonstrate that Mucorales fungi produce a toxin that plays a central role in virulence. Polyclonal antibodies against this toxin inhibit its ability to damage human cells in vitro, and prevent hypovolemic shock, organ necrosis, and death in mice with mucormycosis. RNAi inhibition of the toxin in Rhizopus delemar, compromises the ability of the fungus to damage host cells and attenuates virulence in mice. This 17 kDa toxin has structural and functional features of the plant toxin, ricin, including the ability to inhibit protein synthesis by its N-glycosylase activity, the existence of a motif that mediates vascular leak, and a lectin sequence. Antibodies against the toxin inhibit R. delemar- or toxin-mediated vascular permeability in vitro and cross-react with ricin. A monoclonal anti-ricin B chain antibody binds to the toxin and also inhibits its ability to cause vascular permeability. Therefore, we propose the name “mucoricin” for this toxin. Not only is mucoricin important in the pathogenesis of mucormycosis but our data suggest that a ricin- like toxin is produced by organisms beyond the plant and bacterial kingdoms. Importantly, mucoricin should be a promising therapeutic target.
Low Dielectric Constant Glass-Ceramic Composite Controled Isolated Porosity (1992)

Low dielectric constant glass-ceramic composite for high speed LSI packaging substrates has been developed by introducing isolated pores. Pore structure control can be achieved through the addition of polymer spheres to ceramic powders followed by subsequent thermal decomposition. The dielectric constant of the glass-ceramic composite, composed of quartz glass, cordierite and borosilicate glass having thermal expansion coefficient matching with that for Si chips, can be reduced to 2.9 by the introduction of 26% porosity. When applying such porous materials to multilayer substrates, it is important to consider how pores are isolated and whether or not they are sphere shaped, small and uniformly distributed, in order to prevent degradation in dielectric and mechanical reliabilities. However, the isolated porosity limit is about 13% and the dielectric constant can be reduced to 3.4. Besides, application to glass-ceramic composites composed of quartz glass and borosilicate glass has resulted in reduction in the dielectric constant (3.2). In addition, by using of a green sheet lamination technique, low dielectric constant multilayer glass-ceramic substrate with wiring was developed. The propagation delay can be reduced to 6.5ns/m.
Strategy for the Design of Custom cDNA Microarrays (2003)

DNA microarrays are valuable but expensive tools for expression profiling of cells, tissues, and organs. The design of custom microarrays leads to cost reduction without necessarily compromising their biological value. Here we present a strategy for designing custom cDNA microarrays and constructed a microarray for mouse immunology research (ImmunoChip). The strategy used interrogates expressed sequence tag databases available in the public domain but overcomes many of the problems encountered. Immunologically relevant clusters were selected based on the expression of expressed sequence tags in relevant libraries. Selected clusters were organized in modules, and the best representative clones were identified. When tested, this microarray was found to have minimal clone identity errors or phage contamination and identified molecular signatures of lymphoid cell lines. Our proposed design of custom microarrays avoids probe redundancy, allows the organization of the chip to optimize chip production, and reduces microarray production costs. The strategy described is also useful for the design of oligonucleotide microarrays.