gebhart/patrepeval huggingface dataset:
uv Studies of Tetrahedral Bonding in Diamondlike Amorphous Carbon (1997)

We report ultraviolet (uv) Raman scattering studies of hydrogen-free, diamondlike amorphous carbon thin films with a wide range of tetrahedral bonding. The uv Raman spectra are shown to provide direct evidence for the presence of s${\mathrm{p}}^{3}$-bonded C atoms in these materials. The experimental results are found to be in excellent agreement with theoretical predictions and contribute to an improved understanding of the mechanism by which the diamondlike fraction develops within the amorphous carbon network.
Distant cousins: genomic and sequence diversity within the BPI fold-containing (BPIF)/PLUNC protein family (2011)

PLUNC (palate, lung and nasal epithelium clone) proteins make up the largest branch of the BPI (bactericidal/permeability-increasing protein)/LBP (lipopolysaccharide-binding protein) family of lipid-transfer proteins. PLUNCs make up one of the most rapidly evolving mammalian protein families and exhibit low levels of sequence similarity coupled with multiple examples of species-specific gene acquisition and gene loss. Vertebrate genomes contain multiple examples of genes that do not meet our original definition of what is required to be a member of the PLUNC family, namely conservation of exon numbers/sizes, overall protein size, genomic location and the presence of a conserved disulfide bond. This suggests that evolutionary forces have continued to act on the structure of this conserved domain in what are likely to be functionally important ways.
Simultaneous determination of flucytosine and fluorouracil in human plasma by high‐performance liquid chromatography (2001)

A validated, sensitive and precise reversed-phase high-performance liquid chromatographic method for the simultaneous determination of 5-flucytosine (5-FC) and 5-fluorouracil (5-FU) in human plasma is described. Two compounds, 5-methylcytosine (5-MC) and 5-chlorouracil (5-CU), were used as internal standards for the determination of 5-FC and 5-FU, respectively. Plasma samples were deproteinized with trichloroacetic acid and chromatographed on an octylsilica column, maintained at 30 degrees C during elution, using a 0.04 M phosphate buffer, pH 7.0, as eleunt. Spectrophotometric diode array detection was used at 266 nm. 5-FC, 5-FU, 5-MC and 5-CU were found to have retention times of 4.8, 5.8, 7.7 and 11.0 min respectively. Recoveries of 91-120% with reproducibility and repeatability coefficients of variation of 0.8-6% were obtained. Mean correlation coefficients of 0.99989 and 0.9995 were found for the linear calibration curves (n = 2) of 5-FC (4.816-192.6 mg/l) and 5-FU (0.05368-5.368 mg/l), respectively. The limits of quantitation were 0.3 mg/l for 5-FC and 0.05 mg/l for 5-FU.
Analysis of gene function of bacteriophage phi 29 of Bacillus subtilis: identification of cistrons essential for viral assembly (1976)

Restrictive infection of Bacillus subtilis by suppressor-sensitive (sus) mutants of phi 29 has been used to search for cistrons that function in viral assembly. The products of cistrons 7, 9, 10, and 16 are necessary for head morphogenesis. The neck upper collar protein P10 and the tail protein P9 must be present for DNA packaging to occur. The protein P7 must be present for phage-related particles to form. A prohead-like particle has been isolated during 16-restrictive infection. The particle is composed of the proteins Hd, P10, F, and P7. P16 must function for DNA-filled particles to accumulate. A DNA-containing particle produced in the absence of the cistron 11 product may be an intermediate in the phi 29 assembly pathway. The protein P13 interacts with P9 and P11 to form a stable DNA-filled particle. The products of cistrons 2 and 3 are essential for viral DNA synthesis, and in their absence virus-related particles are not detected.
Application of the grey-box approach to parameter estimation in physicochemical models (2002)

It is demonstrated that the grey-box concept can be applied to certain rigorous models. The principle idea is to simplify the model structure (by means of time discretization and, if necessary, transformation) such that it takes on a form familiar in the black-box approach. In parallel, relevant process characteristics are highlighted and transformed to provide grey-box model constraints. As a result, the parameter estimation becomes more transparent and can be obtained at considerably lower computational costs than is possible with standard physical parameter estimation. In addition, more general stochastic model components (i.e. system noise) can be handled with relative ease. A drawback is the nontrivial formulation of a convex hull of the admissible parameter region associated with the (discrete-time) grey-box model. The merits and pitfalls of this approach are demonstrated with kinetic parameter estimation in a continuous and a batch reactor model. >
Countering Network Worms Through Automatic Patch Generation (2005)

To counter zero-day worms that exploit software flaws such as buffer overflows, this end-point architecture uses source code transformations to automatically create and test software patches for vulnerable segments of targeted applications.
Allosteric Properties of Cyanobacterial Cytochrome c Oxidase: Inhibition of the Coupled Enzyme by ATP and Stimulation by ADP (1999)

Thorough analysis of the cta operon of Synechocystis sp. PCC6803 (grown in high-concentration salt medium to enhance the expression of respiratory proteins) showed that, apart from ctaCDE and Fb genes potentially encoding subunits I, II, III, and a small pseudo-bacteria-like subunit-IV of unknown function, a large mitochondria-like cta-Fm gene and a pronounced terminator structure are additional components of the operon. The deduced cta Fm gene product shows approximately 50% and 20% sequence identity to the Saccharomyces cerevisiae and beef heart mitochondrial COIV proteins, respectively. It also shows amino acid regions (near the N terminus, on the cytosolic side) with conspicuous sequence similarities to adenylate-binding proteins such as ATP synthase beta subunit Walker A and B consensus regions or to adenylate kinase. We suggest that, similar to the situation with beef heart mitochondria, it is the mitochondria-like subunit-IV of the cyanobacterial aa3-type cytochrome-c oxidase that confers allosteric properties to the cyanobacterial enzyme, the H+/e- ratios of cytochrome c oxidation being significantly lowered by ATP (intravesicular or intraliposomal) but enhanced by ADP. Therefore, the antagonistic action of ATP and ADP was in a way that the redox reaction proper, was always significantly less affected than the coupled proton translocation. Evolutionary and ecological implications of the unusual allosteric regulation of a prokaryotic cytochrome-c oxidase is discussed.
Room-temperature continuous-wave vertical-cavity single-quantum-well microlaser diodes (1989)

Room-tempeTature continuous and pulsed lasing of verticalcavity, single-quantum-well, surface-emitting microlasers is achieved at μ983nm. The active Ga0.8In0.2As single quantum well is 100 Å thick. These microlasers have the smallest gain medium volumes among lasers ever built. The entire laser structure is grown by molecular beam epitaxy and the microlasers are formed by chemically assisted ionbeam etching. The microlasers are 3–50μm across. The minimum threshold currents are 1.1 mA (pulsed) and l.5mA(CW).
CD147 and MCT1‐potential partners in bladder cancer aggressiveness and cisplatin resistance (2014)

The relapsing and progressive nature of bladder tumors, and the heterogeneity in the response to cisplatin-containing regimens, are the major concerns in the care of urothelial bladder carcinoma (UBC) patients. The metabolic adaptations that alter the tumor microenvironment and thus contribute to chemoresistance have been poorly explored in UBC setting. We found significant associations between the immunoexpressions of the microenvironment-related molecules CD147, monocarboxylate transporters (MCTs) 1 and 4, CD44 and CAIX in tumor tissue sections from 114 UBC patients. The presence of MCT1 and/or MCT4 expressions was significantly associated with unfavorable clinicopathological parameters. The incidence of CD147 positive staining significantly increased with advancing stage, grade and type of lesion, and occurrence of lymphovascular invasion. Similar associations were observed when considering the concurrent expression of CD147 and MCT1. This expression profile lowered significantly the 5-year disease-free and overall survival rates. Moreover, when selecting patients who received platinum-based chemotherapy, the prognosis was significantly worse for those with MCT1 and CD147 positive tumors. CD147 specific silencing by small interfering RNAs (siRNAs) in UBC cells was accompanied by a decrease in MCT1 and MCT4 expressions and, importantly, an increase in chemosensitivity to cisplatin. Our results provide novel insights for the involvement of CD147 and MCTs in bladder cancer progression and resistance to cisplatin-based chemotherapy. We consider that the possible cooperative role of CD147 and MCT1 in determining cisplatin resistance should be further explored as a potential theranostics biomarker.
Laminar Burning Velocity of Stoichiometric CH4/air Premixed Flames at High-Pressure and High-Temperature (2005)

Experimental and numerical studies on laminar burning velocities of stoichiometric CH4/air flames were performed at high pressure and high temperature. A stoichiometric CH4/air mixture was diluted by helium in order to restrain the intrinsic flame instabilities occurring at high pressure. Measurements of laminar burning velocity for burner-stabilized flames were conducted by a technique employing particle tracking velocimetry (PTV) and planar laser induced fluorescence for OH radical (OH-PLIF) simultaneously, which measures the instantaneous local burning velocity. Laminar burning velocities were determined by the average values of local burning velocities in the region where the Karlovitz number are sufficiently small, meaning that the effect of flame stretch and curvature can be neglected. Numerical simulations were also conducted using a one-dimensional premixed flame code. Detailed reaction mechanisms and the 4-step reduced mechanism were examined, and their results were compared with experimental results to investigate the feasibility of predicting the flame characteristics at high pressure and high temperature, based on the reaction mechanisms.
Metamorphosis of arbitrary triangular meshes (2000)

Three-dimensional metamorphosis (or morphing) establishes a smooth transition from a source object to a target object. The primary issue in 3D metamorphosis is to establish surface correspondence between the source and target objects, by which each point on the surface of the source object maps to a point on the surface of the target object. Having established this correspondence, we can generate a smooth transition by interpolating corresponding points from the source to the target positions. We handle 3D geometric metamorphosis between two objects represented as triangular meshes. To improve the quality of 3D morphing between two triangular meshes, we particularly consider the following two issues: 1) metamorphosis of arbitrary meshes; 2) metamorphosis with user control. We can address the first issue using our recently proposed method based on harmonic mapping (T. Kanai et al., 1998). In that earlier work, we developed each of the two meshes (topologically equivalent to a disk and having geometrically complicated shapes), into a 2D unit circle by harmonic mapping. Combining those two embeddings produces surface correspondence between the two meshes. However, this method doesn't consider the second issue: how to let the user control surface correspondence. The article develops an effective method for 3D morphing between two arbitrary meshes of the same topology. We extend our previously proposed method to achieve user control of surface correspondence.
Aldose reductase inhibitor ranirestat significantly improves nerve conduction velocity in diabetic polyneuropathy: A randomized double‐blind placebo‐controlled study in Japan (2018)

Ranirestat (40 mg/day) was well tolerated and improved nerve conduction velocity. Regarding symptoms and signs, no detectable benefits over the placebo were observed in the ranirestat group during the 52 weeks of treatment.
Single target sputtering of superconducting YBa2Cu3O7−δ thin films on Si (100) (1989)

Thin films of YBa2Cu3O7−δ have been grown on (100) silicon substrates by single target rf diode sputtering. Yttria-stabilized zirconia buffer layers were used to minimize substrate-film reactions. Off-stoichiometric targets were used to compensate for differences between film and target stoichiometries. The composition of the superconducting layer is also influenced by post-deposition anneals, with films closer to the desired stoichiometry resulting from the higher temperature anneals. Film thicknesses spanned the 0.5–2.0 μm range and the onset and zero resistance ( ρ&lt;10−7 Ω cm) temperatures were found to be 95 and 70 K, respectively, for 1.8-μm-thick films.
Magicwand: an intuitive gesture remote control for home appliances (2005)

Mickey Mouse controls several brooms using a magic wand in a Disney movie. Can we control home appliances in a similar way? MagicWand makes this dream comes true. Using MagicWand, we can operate more than one home appliance by intuitive actions. MagicWand has two 3-axis accelerometers to detect a control target by just pointing and to recognize command actions which are classified into some intuitive attributes common to multiple home appliances. It generates the control commands and sends them to the targeted home appliances via Bluetooth. We have connected it with the home network appliance system 'FEMINITY/spl trade/' and realized actual operation of air conditioner, lights, and so on.
Molecular Detection of Human Papillomavirus and Ki-Ras Codon 12 Mutations in Cervical Carcinoma Patients from Hong Kong (1996)

Tumor tissues form a total of 44 patients diagnosed with cervical carcinoma were analyzed fro the presence of human Papillomavirus (HPV) subtypes by polymerase chain reaction (PCR) typing techniques. Consensus primers revealed HPV infection in 34 of 44(77%) patients. Of these HPV strains detected, 19 (43%) were HPV-16 and 4(9%) were HVP-18. The remaining 11 strains (23%) could not be classified as either of these two common subtypes. In addition to the presence of HPV subtypes the specimens were also analyzed for mutation sin the Ki-ras codon 12 oncogene by PCR-based restriction fragment length polymorphism (RFLP) techniques. Only one mutation was detected in 42 specimes (2.4%). There is a high incidence (77%) of HPV infection and the low incidence (2.4%) of Ki-ras oncogene mutations in Hong cervical carcinoma patients.
An Efficient Neural-Network-Based Microseismic Monitoring Platform for Hydraulic Fracture on an Edge Computing Architecture (2018)

Microseismic monitoring is one of the most critical technologies for hydraulic fracturing in oil and gas production. To detect events in an accurate and efficient way, there are two major challenges. One challenge is how to achieve high accuracy due to a poor signal-to-noise ratio (SNR). The other one is concerned with real-time data transmission. Taking these challenges into consideration, an edge-computing-based platform, namely Edge-to-Center LearnReduce, is presented in this work. The platform consists of a data center with many edge components. At the data center, a neural network model combined with convolutional neural network (CNN) and long short-term memory (LSTM) is designed and this model is trained by using previously obtained data. Once the model is fully trained, it is sent to edge components for events detection and data reduction. At each edge component, a probabilistic inference is added to the neural network model to improve its accuracy. Finally, the reduced data is delivered to the data center. Based on experiment results, a high detection accuracy (over 96%) with less transmitted data (about 90%) was achieved by using the proposed approach on a microseismic monitoring system. These results show that the platform can simultaneously improve the accuracy and efficiency of microseismic monitoring.
Identification of HPHT-Treated Yellow to Green Diamonds (2000)

Greenish yellow to yellowish green HPHT-treated diamonds exhibited highly saturated body color, well-defined brown to yellow octahedral graining, and visual evidence of heating, among other characteristics.
Influence des ions monohydrogénophosphates et fluorophosphates sur les propriétés des phosphogypses et la réactivité des phosphoplâtres. (2007)

240 pages
Scaling in ecotoxicology: Theory, evidence and research needs (2005)

Issues of scale, particularly extrapolations across spatial or temporal scales, are of great importance in ecotoxicology. A variety of approaches are valuable for addressing issues of scale, and in developing scaling relationships that allow for extrapolation across scales. Both theoretical and empirical approaches can be applied in deriving scaling relationships. A variety of models may be employed, including statistical models, mathematical models, computer simulations and physical models (such as microcosms and mesocosms). Specific approaches that appear promising include dimensional analysis, allometric scaling, fractal geometry and microcosm/mesocosm experiments in which scale is appropriately manipulated as a treatment variable. Further research should be directed toward assessing the strengths and weaknesses of these approaches, identifying non-linearities and thresholds in scaling relationships, integrating spatial and temporal aspects of scaling, and designing experimental studies that explicitly address scale issues.
Wearable motion sensors to continuously measure real-world physical activities (2013)

Given the progressively falling cost of miniaturized wearable gyroscopes, accelerometers, and other physiologic sensors, as well as inexpensive data transmission, sensing systems may become as ubiquitous as cell phones for healthcare. Neurorehabilitation can develop these mobile health platforms for daily care and clinical trials to improve exercise and fitness, skills learning, and physical functioning.
Embryonic Stem Cells as a Developmental Model in vitro (1999)

Pluripotent mouse embryonic stem (ES) cells have been widely used to create mutant mice that pass genetic modifications to their offspring through germ-line transmission. ES cells also have the capacity to differentiate in vitro into cells of endo- dermal, ectodermal and mesodermal lineages. This special issue of Cells Tissues Organs cont
Synthesis and properties of oligonucleotide-cellulose columns (1972)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis and properties of oligonucleotide-cellulose columnsCaroline R. Astell and Michael SmithCite this: Biochemistry 1972, 11, 22, 4114–4120Publication Date (Print):October 1, 1972Publication History Published online1 May 2002Published inissue 1 October 1972https://pubs.acs.org/doi/10.1021/bi00772a014https://doi.org/10.1021/bi00772a014research-articleACS PublicationsRequest reuse permissionsArticle Views73Altmetric-Citations37LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Size- and Stereochemistry-Dependent Transcriptional Bypass of DNA Alkyl Phosphotriester Adducts in Mammalian Cells (2022)

Environmental, endogenous and therapeutic alkylating agents can react with internucleotide phosphate groups in DNA to yield alkyl phosphotriester (PTE) adducts. Alkyl-PTEs are induced at relatively high frequencies and are persistent in mammalian tissues; however, their biological consequences in mammalian cells have not been examined. Herein, we assessed how alkyl-PTEs with different alkyl group sizes and stereochemical configurations (<i>S</i> <sub>P</sub> and <i>R</i> <sub>P</sub> diastereomers of Me and <i>n</i>Pr) affect the efficiency and fidelity of transcription in mammalian cells. We found that, while the <i>R</i> <sub>P</sub> diastereomer of Me- and <i>n</i>Pr-PTEs constituted moderate and strong blockages to transcription, respectively, the <i>S</i> <sub>P</sub> diastereomer of the two lesions did not appreciably perturb transcription efficiency. In addition, none of the four alkyl-PTEs induced mutant transcripts. Furthermore, polymerase η assumed an important role in promoting transcription across the <i>S</i> <sub>P</sub>-Me-PTE, but not any of other three lesions. Loss of other translesion synthesis (TLS) polymerases tested, including Pol κ, Pol ι, Pol ξ and REV1, did not alter the transcription bypass efficiency or mutation frequency for any of the alkyl-PTE lesions. Together, our study provided important new knowledge about the impact of alkyl-PTE lesions on transcription and expanded the substrate pool of Pol η in transcriptional bypass.
2-Methyl-thiazolidine-2,4-dicarboxylic acid protects against paracetamol induced toxicity in human liver derived HepG2 cells. (1997)

The effects of 2-methyl-thiazolidine-2,4-dicarboxylic acid (CP) on paracetamol-induced toxicity were investigated and evaluated in a human liver derived HepG2 cell line. Incubation of the cells with CP (2 mM and 10 mM) drastically attenuated the GSH and cysteine depletion caused by toxic concentrations of paracetamol (1 mM and 5 mM). When CP (10 mM) was introduced alone into the medium, the level of malondialdehyde and the reactive oxygen species were maintained at the control levels with a simultaneous increase of non-protein sulfhydryl in the cells. Thus, the results of our work prove that CP is a non-toxic precursor of cysteine and GSH, and successfully prevents paracetamol toxicity in HepG2 cells.