gebhart/patrepeval huggingface dataset:
Manufacture of Measles Viruses (2011)

Measles viruses have shown potent oncolytic activity as a therapeutic against a variety of human cancers in animal models and are currently being tested in clinical trials in patients. In contrast to using measles virus as a vaccine, oncolytic activity depends on high concentrations of infectious virus. For use in humans, the high-titer measles virus preparations must also be purified to remove significant levels of cellular proteins and nucleic acid resulting from the cytolytic products of measles virus replication and release. Pleomorphic measles virus must be treated as >1-μm particles that are extremely shear sensitive to maximize recoveries and retain infectivity. Therefore, to maximize the recovery of sterile, high titer infectious measles viruses, the entire production and purification process must be done using gentle conditions and aseptic processing. Here we describe a procedure applicable to the production of small (a few liters) to large (50-60 L) batches of measles virus amplified in Vero cells adapted to serum-free growth. Cell culture supernatant containing the measles virus is clarified by filtration to remove intact Vero cells and other debris, and then treated with Benzonase(®) in the presence of magnesium chloride to digest contaminating nucleic acid. The measles virus in the treated cell culture supernatant is then concentrated and purified using tangential flow filtration (TFF) and diafiltration. The concentrated and diafiltered measles virus is passed through a final clarifying filter prior to final vialing and storage at <-65°C. An infectivity assay to quantify infectious measles virus concentration based on the TCID(50) method is also described. This procedure can be readily adapted to the production and purification of measles viruses using good manufacturing practices (GMP).
Outcomes of Primary and Secondary Tracheoesophageal Puncture: A 16-year Retrospective Analysis (2006)

The current study retrospectively reviewed the cases of 68 patients who had undergone total laryngectomy and tracheoesophageal puncture (TEP) over a 16-year period. Fifty-one patients underwent primary TEP and 17 underwent secondary TEP. Nearly 80% of patients who received TEP at the time of laryngectomy achieved excellent voice quality perceptually. In contrast, only 50% of secondary TEP patients achieved excellent voice ratings. This difference was statistically robust (p = 0.03). Although both surgical and prosthesis-related complications occurred more frequently following primary TEP, statistically significant differences were not achieved. Neither pre- nor postoperative radiotherapy had any effect on voice restoration or complication rates. Based on these data, primary TEP may be preferable for several reasons, including a greater likelihood of successful voice restoration, a shorter duration of postoperative aphonia, and the elimination of the need for a second operation and interim tube feedings.
Applying a belief rule‐base inference methodology to a guideline‐based clinical decision support system (2009)

Abstract: A critical issue in the clinical decision support system (CDSS) research area is how to represent and reason with both uncertain medical domain knowledge and clinical symptoms to arrive at accurate conclusions. Although a number of methods and tools have been developed in the past two decades for modelling clinical guidelines, few of those modelling methods have capabilities of handling the uncertainties that exist in almost every stage of a clinical decision‐making process. This paper describes how to apply a recently developed generic rule‐base inference methodology using the evidential reasoning approach (RIMER) to model clinical guidelines and the clinical inference process in a CDSS. In RIMER, a rule base is designed with belief degrees embedded in all possible consequents of a rule. Such a rule base is capable of capturing vagueness, incompleteness and non‐linear causal relationships, while traditional IF–THEN rules can be represented as a special case. Inference in such a rule base is implemented using the evidential reasoning approach which has the capability of handling different types and degrees of uncertainty in both medical domain knowledge and clinical symptoms. A case study demonstrates that employing RIMER in developing a guideline‐based CDSS is a valid novel approach.
Electric Low Pressure Fuel Pump Control for Fuel Saving (2013)

<div class="section abstract"><div class="htmlview paragraph">The trend of CO2 emission limits and the fuel saving due to the oil price increase are important drivers for engines development. The involved technologies have the aim to improve the global engine efficiency, improving combustion and minimizing energy losses. The engine auxiliary devices electrification (i.e. cooling pump or lubricating pump) is a way to reduce not useful energy consumption, because it becomes possible to control them depending on engine operating point. This kind of management can be applied to the electric low pressure fuel pump. Usually the fuel delivery is performed at the maximum flow rate and a pressure regulator discharges the exceeding fuel amount inside the rail (i.e. gasoline engine) or upstream of the high pressure pump (i.e. common rail diesel engine). At part load, especially in diesel application, the electric fuel pump flow is higher than needed for engine power generation. In this work it has been investigated the fuel saving achievable through a proper control of the electric fuel pump on a small common rail diesel engine, and a control architecture is proposed. The aim was to maximize the fuel economy without any impact on pressure control in the engine transient conditions. The results on engine test bench and on vehicle have confirmed the target aimed.</div></div>
Approach of Development of Electric Double Layer Capacitor for High Power and Long Life (2007)

We developed an electric double layer capacitor using an ionic liquid as the electrolyte salt for a high energy density and a life performance. The influence of the surface area of the activated carbon and the concentration of the electrolyte on the storage and life performance of the capacitor was studied. Separator can control the gas generation during the float charge of the capacitor to be able to extend the its life. Our produced capacitor, “N’s Cap”, by these techniques has good performances and the storage module assembled with the cells and the external circuit was presented.
Visible-Light-Activated Catalytic Enantioselective β-Alkylation of α,β-Unsaturated 2-Acyl Imidazoles Using Hantzsch Esters as Radical Reservoirs (2018)

An efficient and practical method for the enantioselective β-functionalization of α,β-unsaturated 2-acyl imidazoles is described. The method uses a previously devised chiral-at-metal rhodium catalyst (Λ-RhS, 4 mol %) along with Hantzsch ester derivatives as alkyl radical sources. The rhodium complex exerts a dual role as the visible-light-absorbing unit upon substrate binding and as the asymmetric catalyst. The method provides up to quantitative yields with excellent enantioselectivities up to 98% ee and can be classified as a redox-neutral, electron-transfer-catalyzed reaction.
Advanced ECG processor with HRV analysis for real-time portable health monitoring (2011)

In this paper, a portable ECG processor designed for health monitoring applications is proposed. The ECG processor acquires three-channel ECG raw data through a front-end circuit, and measures the time between successive heart beats on lead II as RR intervals for heart rate variability (HRV) analysis. Functions such as R-peak detection, RR interval calculation, sliding memory window scheme, and time-frequency analysis of HRV have also been developed. A real-time HRV analysis processor is realized by employing a Lomb periodogram for time-frequency power spectral density (PSD) analysis of the heart rate. The Lomb time-frequency distribution (TFD) is suited for deriving the PSD of unevenly spaced data sets. The system has been implemented in hardware and verified on FPGA.
Models and methods for automated material identification in hyperspectral imagery acquired under unknown illumination and atmospheric conditions (1999)

The spectral radiance measured by an airborne imaging spectrometer for a material on the Earth's surface depends strongly on the illumination incident of the material and the atmospheric conditions. This dependence has limited the success of material-identification algorithms that rely on hyperspectral image data without associated ground-truth information. In this paper, the authors use a comprehensive physical model to show that the set of observed 0.4-2.5 /spl mu/m spectral-radiance vectors for a material lies in a low-dimensional subspace of the hyperspectral-measurement space. The physical model captures the dependence of the reflected sunlight, reflected skylight, and path-radiance terms on the scene geometry and on the distribution of atmospheric gases and aerosols over a wide range of conditions. Using the subspace model, they develop a local maximum-likelihood algorithm for automated material identification that is invariant to illumination, atmospheric conditions, and the scene geometry. The algorithm requires only the spectral reflectance of the target material as input. The authors show that the low dimensionality of material subspaces allows for the robust discrimination of a large number of materials over a wide range of conditions. They demonstrate the invariant algorithm for the automated identification of material samples in HYDICE imagery acquired under different illumination and atmospheric conditions.
Monitoring of Therapeutic Concentrations of Psychotropic Drugs in Plasma by Radioimmunoassays (1978)

Sensitive and rapid radioimmunoassay (RIA) procedures for the determination of plasma concentrations of psychotropic drugs—chlorpromazine, Imipramlne, desipramlne, amitriptyline, and nortriptyline—are described. These procedures employing 20 to 200µl of plasma can determine subnanogram concentrations of these drugs and are shown to be suitable for therapeutic monitoring of these drugs in patients. For the antidepressant drugs, the RIA procedures were found to compare favorably with gas chromatographic procedures employing alkali flame ionization detectors. The RIA procedure for chlorpromazine measures chlorpromazine as well as its active metabolite, 7-hydroxychlorpromazine. Cross-reactivity profiles for the antiserum of each drug were obtained, and in each case the antiserum of the drug distinguished the drug from its inactive metabolites and most of the other psychotropic drugs tested.
Wikipedia2Vec: An Efficient Toolkit for Learning and Visualizing the Embeddings of Words and Entities from Wikipedia (2020)

Ikuya Yamada, Akari Asai, Jin Sakuma, Hiroyuki Shindo, Hideaki Takeda, Yoshiyasu Takefuji, Yuji Matsumoto. Proceedings of the 2020 Conference on Empirical Methods in Natural Language Processing: System Demonstrations. 2020.
MCMICRO: A scalable, modular image-processing pipeline for multiplexed tissue imaging (2021)

ABSTRACT Highly multiplexed tissue imaging makes molecular analysis of single cells possible in a preserved spatial context. However, reproducible analysis of the underlying data poses a substantial computational challenge. Here we describe a modular and open-source computational pipeline (MCMICRO) for performing the sequential steps needed to transform large, multi-channel whole slide images into single-cell data. We demonstrate use of MCMICRO on images of different tissues and tumors acquired using multiple imaging platforms, thereby providing a solid foundation for the continued development of tissue imaging software.
Phase Behavior of Ionic Liquid−LiX Mixtures:  Pyrrolidinium Cations and TFSI<sup>-</sup> Anions (2004)

Phase diagrams are reported for mixtures between bis(trifluoromethanesulfonyl)imide (TFSI-) salts containing Li+ and N-alkyl-N-methylpyrrolidinium (PYR1R+) cations. The latter salts readily form both room temperature ionic liquids and plastic crystalline phases. Mixed salt crystalline phases are found which are likely to influence the performance of such mixed salt systems when utilized as electrolytes for electrochemical devices as well as give insight into ionic liquid−solute interactions.
Detrimental Hemodynamic Effects of Nitric Oxide Synthase Inhibition in Septic Shock (1994)

The potentiation of LPS-induced pulmonary hypertension following NAME infusion suggests that inhibition of nitric oxide synthase may have a limited role in the treatment of septic shock.
The Hypertonic State (1977)

Evolution has provided an elaborate network of regulatory systems that stabilize body-fluid volume, temperature and composition. This stability insulates cells from an ambient hostile world and assures them the best possible conditions in which to function optimally. When one of these regulatory systems fails, cells and even whole organ systems cannot function properly. This paper discusses what goes wrong when body fluids become too concentrated.Cell membranes are more or less impermeable to most solutes but freely permeable to water so that intracellular-fluid and extracellular-fluid osmolality equalizes despite very different composition (Fig. 1). The major osmotic solutes in intracellular fluid . . .
Simian Immunodeficiency Virus Infection of Macaques: End-Stage Disease Is Characterized by Widespread Distribution of Proviral DNA in Tissues (1991)

Four simian immunodeficiency virus (SIV)-infected macaques in the terminal stages of AIDS were studied. Easily detectable unintegrated proviral DNA was present in nearly every tissue (lymphoid and nonlymphoid) examined. SIV-specific immunohistochemistry revealed that macrophages or macrophage-like cells were the predominant reservoirs for SIV. High burdens of infectious SIV were also characteristic of end-stage disease. At the molecular level, sequence analyses of molecular clones derived directly from tissue demonstrated that SIV, like other RNA viruses, exists as a mixture of closely related genomes. Viruses derived by cocultivation seemed to be selected for their ability to grow in tissue culture and did not represent the wide spectrum of genotypes in tissues. These data indicate that SIV-induced immunodeficiency is a complex, multisystem disease and that SIV replication, in addition to impairing the immune system, may have direct deleterious effects on multiple organ systems.
“感知世界”特辑——美国电化学会特许授权转载——感知生命，感知世界 (2012)

本期刊出的“感知世界”特辑原载于美国电化学会会员刊物《INTERFACE》第19卷第4期，是美国电化学会传感器学组为该刊编辑的“Sense the World”专辑。主持该专辑的客座编辑是PeterHesketh教授和JingLi（李晶）博士，他们长期从事化学传感器和相关领域的研究，是美国有声望的化学传感技术专家，都曾担任过美国电化学会传感器学组的主席。三篇专稿的主题都与经济和社会发展以及环境保护和国家安全密切相关，作者也都是各自研究领域成就卓著的知名专家。他们在文中对各自研究领域的现状、面临的挑战以及未来发展都作了详尽和精辟的评述，并揭示了传感技术在各该领域中不可或缺的作用以及有待创新发展的生长点。
Stability of Cyclophosphamide in Extemporaneous Oral Suspensions (2010)

Cyclophosphamide can be extemporaneously prepared in simple syrup or Ora-Plus and stored for at least 2 months under refrigeration without significant degradation.
The radionuclide renogram as a predictor of relative renal blood flow. (1983)

Radionuclide renograms obtained with Tc-99m-DTPA were used to calculate relative renal blood flow in 16 dogs. In 13 of these dogs, relative blood flow ratios (R:L) calculated from the renograms were correlated with ratios measured with an electromagnetic flowmeter or with radiolabeled microspheres. The results indicate that Tc-99m-DTPA renograms can be used to make an accurate prediction of relative renal blood flow.
Adsorption Equilibrium of Methane, Carbon Dioxide, and Nitrogen on Zeolite 13X at High Pressures (2004)

High-pressure adsorption of methane, carbon dioxide, and nitrogen on zeolite 13X was measured in the pressure range (0 to 5) MPa at (298, 308, and 323) K and fitted with the Toth and multisite Langmuir models. Isosteric heats of adsorption were (12.8, 15.3, and 37.2) kJ/mol for nitrogen, methane, and carbon dioxide respectively, which indicate a very strong adsorption of carbon dioxide. The preferential adsorption capacity of CO2 on zeolite 13X was much higher than for the other gases, indicating that zeolite 13X can be used for methane purification from natural gas or for carbon dioxide sequestration from flue gas.
Metastatic Colorectal Cancer: Systemic Treatment in the New Millennium (2003)

Recently completed or ongoing clinical trials to study novel targeting agents have initiated a new era of drug development. Anti-angiogenesis drugs, tyrosine kinase inhibitors, and epidermal growth factor blockers are among this new generation of agents with encouraging preliminary data. Randomized trials will determine the impact of these newer agents on survival and quality of life of patients with metastatic CRC.
A multiauxotrophic CHO cell line for the rapid isolation of producers of diverse or high levels of recombinant proteins (2022)

Chinese Hamster Ovary (CHO) cells are widely used for the high-level production of recombinant proteins. We created a multiauxotrophic mutant of CHO-K1 cells, CHO8A, that is deficient in eight enzymatic steps in the purine/pyrimidine biosynthetic pathways. Prototrophy was restored by transfections with complementary DNA-based genes for the eight missing activities. CHO8A cells permit: (1) selection of transfectant clones that have incorporated genes for eight or more different polypeptides, suitable for engineering complex proteins, or pathways; and (2) the single-step selection of high producers of a particular protein. The latter is achieved by simultaneous use of eight vectors, each bearing one of the eight rescue genes and a cargo protein gene. Screening as few as 10 surviving colonies yielded high producers secreting mAbs at 84 picograms per cell per day or more. CHO8A was isolated by CRISPR-Cas9 knockout of 10 genes in the pathways to pyrimidines (Dhodh, Umps, Ctps1, Ctps2, and Tyms) and purines (Paics, Atic, Impdh1, Impdh2, and Gmps).
Integrated Microwave Photonics (2024)

Integrated photonics has the potential to change the power-scaling laws of high-bandwidth systems through proper architectural choices that combine photonics with electronics to optimize performance, power, footprint, and cost. This chapter outlines the principles of integrated microwave photonics (IMWP). Several material platforms have been employed to implement basic building blocks, devices, and application-specific photonic integrated circuits for MWP applications. Of all the material platforms, only three: indium phosphide, silicon on insulator, and silicon (oxy)nitride have reached the required degree of maturity to be currently considered viable options for the implementation of complex MWP-integrated circuits as they are aligned with generic integration and generic foundry models. The chapter discusses the new emerging topic of multifunctional circuits and the no less exciting area of universal programmable MWP processors, where a single hardware architecture can be programmed to carry out the different functionalities required in MWP applications.
A New Chiral Bidentate (P,S) Ligand for the Asymmetric Intermolecular Pauson−Khand Reaction (2000)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTA New Chiral Bidentate (P,S) Ligand for the Asymmetric Intermolecular Pauson−Khand ReactionXavier Verdaguer, Albert Moyano, Miquel A. Pericàs, Antoni Riera, Miguel Angel Maestro, and José MahíaView Author Information Unitat de Recerca en Síntesi Asimètrica Departament de Química Orgànica, Universitat de Barcelona, Martí i Franquès, 1-11, 08028 Barcelona, Spain Servicios Xerais de Apoio á Investigación Campus Zapateria, s/n, Universidade da Coruña 15071, A Coruña, Spain Cite this: J. Am. Chem. Soc. 2000, 122, 41, 10242–10243Publication Date (Web):September 30, 2000Publication History Received30 May 2000Published online30 September 2000Published inissue 1 October 2000https://pubs.acs.org/doi/10.1021/ja001839hhttps://doi.org/10.1021/ja001839hrapid-communicationACS PublicationsCopyright © 2000 American Chemical SocietyRequest reuse permissionsArticle Views970Altmetric-Citations95LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (2)»Supporting Information Supporting Information SUBJECTS:Cobalt,Group 15 compounds,Ligands,Mixtures,Stereoselectivity Get e-Alerts
Application of built-in adjuvants for epitope-based vaccines (2019)

Several studies have shown that epitope vaccines exhibit substantial advantages over conventional vaccines. However, epitope vaccines are associated with limited immunity, which can be overcome by conjugating antigenic epitopes with built-in adjuvants (e.g., some carrier proteins or new biomaterials) with special properties, including immunologic specificity, good biosecurity and biocompatibility, and the ability to vastly improve the immune response of epitope vaccines. When designing epitope vaccines, the following types of built-in adjuvants are typically considered: (1) pattern recognition receptor ligands (i.e., toll-like receptors); (2) virus-like particle carrier platforms; (3) bacterial toxin proteins; and (4) novel potential delivery systems (e.g., self-assembled peptide nanoparticles, lipid core peptides, and polymeric or inorganic nanoparticles). This review primarily discusses the current and prospective applications of these built-in adjuvants (i.e., biological carriers) to provide some references for the future design of epitope-based vaccines.
Ein neuer Herstellungsweg für Fullerene (1992)

Größere Fullerenmengen bei einer bemerkenswert niedrigen Bildungstemperatur von 2700°C (Heliumdruck 150 hPa) liefert ein neues Syntheseverfahren. Hier‐ bei werden zylindrische Kohlenstofformkörper induktiv geheizt und verdampft, die entstandenen Fullerene aus Ruß extrahiert, und die Produktvertei‐ lung wird massenspektrometrisch ermittelt.