gebhart/patrepeval huggingface dataset:
PARAMETER SELECTION FOR HOMOPOLAR GENERATORS USED AS PULSED ENERGY STORES (1981)

ABSTRACT A method of analysis is presented which shows the role played by collector current density in the design of homopolar generator energy stores (electro-mechanical capacitors). For a required energy storage capacity and peak current, it is shown how the designer may obtain some guidance in the selection of this parameter for five homopolar generator geometries.
Induction of Cancer Stem Cell Properties in Colon Cancer Cells by Defined Factors (2014)

Cancer stem cells (CSCs) are considered to be responsible for the dismal prognosis of cancer patients. However, little is known about the molecular mechanisms underlying the acquisition and maintenance of CSC properties in cancer cells because of their rarity in clinical samples. We herein induced CSC properties in cancer cells using defined factors. We retrovirally introduced a set of defined factors (OCT3/4, SOX2 and KLF4) into human colon cancer cells, followed by culture with conventional serum-containing medium, not human embryonic stem cell medium. We then evaluated the CSC properties in the cells. The colon cancer cells transduced with the three factors showed significantly enhanced CSC properties in terms of the marker gene expression, sphere formation, chemoresistance and tumorigenicity. We designated the cells with CSC properties induced by the factors, a subset of the transduced cells, as induced CSCs (iCSCs). Moreover, we established a novel technology to isolate and collect the iCSCs based on the differences in the degree of the dye-effluxing activity enhancement. The xenografts derived from our iCSCs were not teratomas. Notably, in contrast to the tumors from the parental cancer cells, the iCSC-based tumors mimicked actual human colon cancer tissues in terms of their immunohistological findings, which showed colonic lineage differentiation. In addition, we confirmed that the phenotypes of our iCSCs were reproducible in serial transplantation experiments. By introducing defined factors, we generated iCSCs with lineage specificity directly from cancer cells, not via an induced pluripotent stem cell state. The novel method enables us to obtain abundant materials of CSCs that not only have enhanced tumorigenicity, but also the ability to differentiate to recapitulate a specific type of cancer tissues. Our method can be of great value to fully understand CSCs and develop new therapies targeting CSCs.
Automated classification of optical coherence tomography images of human atrial tissue (2016)

Tissue composition of the atria plays a critical role in the pathology of cardiovascular disease, tissue remodeling, and arrhythmogenic substrates. Optical coherence tomography (OCT) has the ability to capture the tissue composition information of the human atria. In this study, we developed a region-based automated method to classify tissue compositions within human atria samples within OCT images. We segmented regional information without prior information about the tissue architecture and subsequently extracted features within each segmented region. A relevance vector machine model was used to perform automated classification. Segmentation of human atrial ex vivo datasets was correlated with trichrome histology and our classification algorithm had an average accuracy of 80.41% for identifying adipose, myocardium, fibrotic myocardium, and collagen tissue compositions.
Ca2+-dependent deimination-induced disassembly of intermediate filaments involves specific modification of the amino-terminal head domain (1989)

Peptidylarginine deiminase (proteinarginine iminohydrolase, EC 3.5.3.15) converted some arginine residues to citrulline residues in soluble vimentin, in a micromolar Ca2+-dependent manner and resulted in the loss of polymerization competence of the intermediate filament protein. When about 8 mol of residues/mol of vimentin were deiminated, there was a complete loss of filament forming ability. This enzyme also deiminated vimentin filaments which had been polymerized, and deimination of vimentin filaments resulted in filament disassembly. Similar results were obtained with other intermediate filaments such as desmin and glial filaments. High performance liquid chromatography and amino acid analyses of lysine-specific protease-generated fragments from deiminated vimentin (about 8 mol of citrulline/mol of vimentin) showed a differential deimination of three structural domains. The head domain was predominant. These observations suggest that the head domain strongly influences integrity of the intermediate filament.
The Prebiotic Activity of Simulated Gastric and Intestinal Digesta of Polysaccharides from the Hericium erinaceus (2018)

<i>Hericium erinaceus</i> (HE) is a well-known edible and medicinal fungus widely grown in Asian countries. Polysaccharides from the <i>Hericium erinaceus</i> (HEP) are major biological macromolecules. It has been reported that HEP has multiple biological activities, such as antioxidant activity, immunomodulatory effects, anti-inflammatory effect, anti-chronic gastritis activity, and so on. In the current study, we investigated the biological property of HEP during gastrointestinal digestion. The results indicated that both simulated gastric and small intestinal digesta of HEP has better stimulation of probiotics growth than HEP alone, especially for <i>Lactobacillus plantarum</i> BG112. The prebiotic activity was the strongest when HEP was treated by simulated gastric juice for 2 h and by simulated small intestinal juice for 4 h. The molecular weight (M<sub>w</sub>) of HEP decreased from 1.68 × 10⁶ Da and 2.32 × 10⁴ Da to 529.3 ± 7.2 Da, as digestion time increased. Meanwhile, the reducing sugar content was significantly increased from 0.610 ± 0.007 to 22.698 ± 0.752 mg/ml, suggesting that the decrease of M<sub>w</sub> was likely due to the breakdown of glycosidic bonds. Considerable mannose and galactopyranose were released throughout the gastrointestinal digestion period, indicating that the gastrointestinal digestion resulted in production of free monosaccharides. After fermentation of <i>L. plantarum</i> BG112, the M<sub>w</sub> of HEP was decreased and short chain fatty acids (SCFAs) including acetic acid, isovaleric acid, lactic acid, and butyric acid were produced. We speculated that the release of free monosaccharides during gastrointestinal digestion and utilization of HEP, by the probiotics, contributed to the prebiotic activity of HEP's gastric and intestinal digesta. These results unveiled some mechanisms on the close relationship between the structure and bioactivity of polysaccharides, during digestion.
Force treadmill for measuring vertical and horizontal ground reaction forces (1998)

We constructed a force treadmill to measure the vertical, horizontal and lateral components of the ground-reaction forces (Fz, Fy, Fx, respectively) and the ground-reaction force moments (Mz, My, Mx), respectively exerted by walking and running humans. The chassis of a custom-built, lightweight (90 kg), mechanically stiff treadmill was supported along its length by a large commercial force platform. The natural frequencies of vibration were >178 Hz for Fz and >87 Hz for Fy, i.e., well above the signal content of these ground-reaction forces. Mechanical tests and comparisons with data obtained from a force platform runway indicated that the force treadmill recorded Fz, Fy, Mx and My ground-reaction forces and moments accurately. Although the lowest natural frequency of vibration was 88 Hz for Fx, the signal-to-noise ratios for Fx and Mz were unacceptable. This device greatly decreases the time and laboratory space required for locomotion experiments and clinical evaluations. The modular design allows for independent use of both treadmill and force platform.
Counting Answers to Existential Questions (2019)

Conjunctive queries select and are expected to return certain tuples from a relational database. We study the potentially easier problem of counting all selected tuples, rather than enumerating them. In particular, we are interested in the problem’s parameterized and data complexity, where the query is considered to be small or even fixed, and the database is considered to be large. We identify two structural parameters for conjunctive queries that capture their inherent complexity: The dominating star size and the linked matching number. If the dominating star size of a conjunctive query is large, then we show that counting solution tuples to the query is at least as hard as counting dominating sets, which yields a fine-grained complexity lower bound under the Strong Exponential Time Hypothesis (SETH) as well as a #W[2]-hardness result in parameterized complexity. Moreover, if the linked matching number of a conjunctive query is large, then we show that the structure of the query is so rich that arbitrary queries up to a certain size can be encoded into it; in the language of parameterized complexity, this essentially establishes a #A[2]-completeness result.\nUsing ideas stemming from Lovász (1967), we lift complexity results from the class of conjunctive queries to arbitrary existential or universal formulas that might contain inequalities and negations on constraints over the free variables. As a consequence, we obtain a complexity classification that refines and generalizes previous results of Chen, Durand, and Mengel (ToCS 2015; ICDT 2015; PODS 2016) for conjunctive queries and of Curticapean and Marx (FOCS 2014) for the subgraph counting problem. Our proof also relies on graph minors, and we show a strengthening of the Excluded-Grid-Theorem which might be of independent interest: If the linked matching number (and thus the treewidth) is large, then not only can we find a large grid somewhere in the graph, but we can find a large grid whose diagonal has disjoint paths leading into an assumed node-well-linked set.
Plda-based system for text-prompted password speaker verification (2015)

Recently we have proposed a new State-GMM-supervector extractor for solving the problem of text-dependent speaker recognition. We demonstrated that segmenting the passphrase into word states for supervector extraction makes it possible to create more accurate statistical models of speech signals and to achieve reduction of EER compared to the best state-of-the-art systems of text-dependent verification for a text-prompted passphrase. In this paper we used a similar approach for creating a text-dependent verification system based on PLDA. The proposed system is easy to implement. Although the PLDA system is inferior to the baseline systems, fusing it with the baseline systems leads to improved quality of text-prompted password speaker verification in comparison to the fusion of baseline systems.
Incretin-Based Therapies Role in COVID-19 Era: Evolving Insights (2020)

The current coronavirus disease 2019 (COVID-19) pandemic has led the scientific community to breach new frontiers in the understanding of human physiology and disease pathogenesis. It has been hypothesized that the human dipeptidyl peptidase 4 (DPP4) enzyme receptor may be a functional target for the spike proteins of severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2). Since DPP4-inhibitors are currently used for the treatment of patients with type-2 diabetes (T2DM), there is currently high interest in the possibility that these agents, or incretin-based therapies (IBTs) in general, may be of benefit against the new coronavirus infection. Diabetes is associated with increased COVID-19 severity and mortality, and accumulating evidence suggests that IBTs may favorably alter the clinical course of SARS-CoV-2 infection due to their inherent mechanisms of action. Further research into prognostic variables associated with various antidiabetic treatment regimens, and in particular the IBT, in patients with T2DM affected by the COVID-19 pandemic is therefore warranted.
Depth of Interaction With a 3-Dimensional Checkerboard Arrangement LSO-LSO Block (2010)

In order to improve image quality in Positron Emission Tomography (PET) different routes are being pursued such as fast timing resolution for time-of-flight PET, higher spatial resolution by the use of smaller scintillator pixels and the use of depth-of-interaction information. The detection of the depth-of-interaction (DOI) of a gamma ray within a detector, deploying pulse shape discrimination (PSD), has been used to increase sensitivity and spatial resolution, especially at the edge of the field of view (FOV). The DOI information is used to reduce the parallax error; thus improving spatial resolution. Commonly, different scintillator materials with different decay times and light output and other differentiating factors, such as density, emission spectra, etc., are used for DOI detectors. We present a multilayer phoswich detector comprised of LSO with different decay times in the range from 30 ns to 47 ns. The difference in decay times is achieved by co-doping LSO:Ce with Ca, resulting in short decay times of ~30 ns. The use of a cut light guide allows the use of regular Photomultiplier tubes, giving the opportunity of a potential DOI detector replacement for current detectors. We were able to identify each pixel in the different detector layers and thus, to determine the depth of the photon interaction and achieve a timing resolution of 345 ps.
Src family kinases and their role in hematological malignancies (2014)

The Src family protein tyrosine kinases (SFKs) are non-receptor intracellular kinases that have important roles in both hematopoiesis and leukemogenesis. The derangement of their expression or activation has been demonstrated to contribute to hematological malignancies. This review first examines the mechanisms of SFK overexpression and hyperactivation, emphasizing the dysregulation of the upstream modulators. Subsequently, the role of SFK up-regulation in the initiation, progression and therapy resistance of many hematological malignancies is also analyzed. The presented evidence endeavors to highlight the influence of SFK up-regulation on an extensive number of hematological malignancies and the need to consider them as candidates in targeted anticancer therapy.
Statistical modeling tools, methods and applications for integrated circuit manufacturability (2002)

A description of various statistical modeling methods is provided. The analysis methods are discussed to remove the confusion in the existing literature. Statistical models are generated using factor analytic technique for SPICE level 3 on a 0.8 micron LDD CMOS. It is shown that our measurement based approach accurately predicts the device performance. We show that the existence of a "physical MOS model" is not a necessary pre-requisite to perform statistical modeling. Our approach provides models that are suitable for both analog and digital designs and promises to make statistical modeling feasible for general use.
The Role of CDK4/6 Inhibition in Breast Cancer (2015)

Imbalance of the cyclin D and cyclin-dependent kinase (CDK) pathway in cancer cells may result in diversion away from a pathway to senescence and toward a more proliferative phenotype. Cancer cells may increase cyclin D-dependent activity through a variety of mechanisms. Therapeutic inhibition of CDKs in tumors to negate their evasion of growth suppressors has been identified as a key anticancer strategy. In this review, we outline the development of CDK inhibitory therapy in breast cancer, including the initial experience with the pan-CDK inhibitor flavopiridol and the next generation of oral highly selective CDK4 and CDK6 inhibitors PD0332991 (palbociclib), LEE011 (ribociclib), and LY2835219 (abemaciclib). Data from phase I and II studies in estrogen receptor-positive (ER+) breast cancer demonstrate promising efficacy with manageable toxic effects, chiefly neutropenia. We discuss these studies and the phase III studies that are accruing or nearing completion. We describe the application of such therapy to other breast cancer settings, including HER2-positive breast cancer and the adjuvant treatment of early breast cancer. We also discuss potential concerns surrounding the combination of CDK inhibitors with chemotherapy and their effects on repair of double-strand DNA breaks in cancer cells. Oral highly selective CDK inhibitors show great promise in improving the outcomes of patients with ER+ breast cancer, although caution must apply to their combination with other agents and in the early breast cancer setting.
New Anti-Infective Coatings of Medical Implants (2008)

ABSTRACT Implantable devices are highly susceptible to infection and are therefore a major risk in surgery. The present work presents a novel strategy to prevent the formation of a biofilm on polytetrafluoroethylene (PTFE) grafts. PTFE grafts were coated with gentamicin and teicoplanin incorporated into different lipid-like carriers under aseptic conditions in a dipping process. Poly- d , l -lactic acid, tocopherol acetate, the diglyceride Softisan 649, and the triglyceride Dynasan 118 were used as drug carriers. The drug release kinetics, anti-infective characteristics, biocompatibility, and hemocompatibility of the coatings developed were studied. All coatings showed an initial drug burst, followed by a low continuous drug release over 96 h. The dimension of release kinetics depended on the carrier used. All coated prostheses reduced bacterial growth drastically over 24 h, even below pathologically relevant concentrations. Different cytotoxic levels could be observed, revealing tocopherol acetate as the most promising biocompatible carrier. A possible reason for the highly cytotoxic effect of Softisan 649 could be assessed by demonstrating incorporated lipids in the cell soma with Oil Red O staining. Tromboelastography studies, enzyme-linked immunosorbent assays, and an amidolytic substrate assay could confirm the hemocompatibility of individual coatings. The development of the biodegradable drug delivery systems described here and in vitro studies of those systems highlight the most important requirements for effective as well as compatible anti-infective coatings of PTFE grafts. Through continuous local release, high drug levels can be produced at only the targeted area and physiological bacterial proliferation can be completely inhibited, while biocompatibility as well as hemocompatibility can be ensured.
Abnormal response to calmodulin in vitro of dystrophic chicken muscle membrane calcium-ATPase activity (1988)

A skeletal muscle membrane fraction enriched in sarcoplasmic reticulum (SR) contained Ca2+-ATPase activity which was stimulated in vitro in normal chickens (line 412) by 6 nM purified bovine calmodulin (33% increase over control, P less than 0.001). In contrast, striated muscle from chickens (line 413) affected with an inherited form of muscular dystrophy, but otherwise genetically similar to line 412, contained SR-enriched Ca2+-ATPase activity which was resistant to stimulation in vitro by calmodulin. Basal levels of Ca2+-ATPase activity (no added calmodulin) were comparable in muscles of unaffected and affected animals, and the Ca2+ optima of the enzymes in normal and dystrophic muscle were identical. Purified SR vesicles, obtained by calcium phosphate loading and sucrose density gradient centrifugation, showed the same resistance of dystrophic Ca2+-ATPase to exogenous calmodulin as the SR-enriched muscle membrane fraction. Dystrophic muscle had increased Ca2+ content compared to that of normal animals (P less than 0.04) and has been previously shown to contain increased levels of immuno- and bioactive calmodulin and of calmodulin mRNA. The calmodulin resistance of the Ca2+-ATPase in dystrophic muscle reflects a defect in regulation of cell Ca2+ metabolism associated with elevated cellular Ca2+ and calmodulin concentrations.
Structural characteristics of the elliptical masonry dome of the sanctuary of Vicoforte (2003)

The elliptical masonry dome of the Sanctuary ofVicoforte near Mondovl in northwest Italy, built in1731, is the largest dome of this kind in the world(Figures 1-4). With its exceptional dimensions(major and minor internal axes 37,15 and 24,80 m,maximum height of the monument 84 m) it largelysurpasses the dome of S. Andrea del Quirinale (1658)by Bernini (whose dimensions are 25,80, 16,25 and25,80 m, respectively) and a group of other ellipticaldomes both in Rome (S. Carlo by Borromini, 1638,and S. Giacomo by Volterra, 1592) and in Spain (S.Hermenegildo in Cordoba, 1616 and Convento de lasBernardas in Alcala, 1626) with major axes between23 and 25 m and minor axes between 16 and 19 m(Zocca 1945, Escrig, Cobreros and Valcarcel 1997).Figure
Nitric Oxide Production Mediated by Nitrate Reductase in the Green Alga Chlamydomonas reinhardtii: an Alternative NO Production Pathway in Photosynthetic Organisms (2002)

Biological activity of nitric oxide (NO) production was investigated in the unicellular green alga Chlamydomonas reinhardtii. An NO specific electrode detected a rapid increase in signal when nitrite (NO(2)(-)) was added into a suspension of C. reinhardtii intact cells in the dark. The addition of KCN or the NO quencher bovine hemoglobin completely abolished the signal, verifying that the nitrite-dependent increase in signal is due to enzymatic NO production. L-arginine, the substrate for NO synthase, did not induce detectable NO production and the NOS inhibitor N(omega)-nitro-L-arginine showed no inhibitory effect on the nitrite-dependent production of NO. Illuminating cells showed a significant suppressive effect on NO production. When the photosynthetic electron transport inhibitor 3-(3,4-dichlorophenyl)-1,1-dimethylurea was present in the suspension, C. reinhardtii cells produced NO after the addition of nitrite even under illumination. Kinetic and microscopic observations, using the intracellular fluorescent NO probe 4,5-diaminofluorescein-2 diacetate, both demonstrated that NO was produced within the cells in response to the addition of nitrite. The Chlamydomonas mutant cc-2929, which lacks nitrate reductase (NR) activity, did not display any of the responses observed in the wild-type cells. The results presented here provide direct in vivo evidence to confirm that NR is involved in the nitrite-dependent NO production in the green alga.
New Synthesis of 2-Phenylindene Derivatives (1993)

Abstract Aza-ortho-xylylenes 4 generated via thermal extrusion of SO2 from 1,3-dihydro-2,1-benzisothiazolo-3-spiro-2′-indan 2,2-dioxides 3 undergo [1,5] hydrogen shift giving 2-phenylindene derivatives 5 in good yields.
Principles of Hydroxamate Inhibition of Metalloproteases:  Carboxypeptidase A (2000)

Hydroxamic acids of structure RCON(OH)CH(2)CH(CH(2)C(6)H(5))CO(2)H induce micromolar competitive inhibition of catalysis for the enzyme carboxypeptidase A. Enzyme affinity depends on the nature of the acyl group, for RCO equaling HCO, CH(3)CO, FCH(2)CO, F(2)CHCO, F(3)CCO, CH(3)OCH(2)CO, or CH(3)OCO. In acid dissociation these residues yield hydroxamic acid pK(a) values that vary from 7.6 to 10.3. Profiles of inhibitory pK(i) plotted versus pH indicate characteristically a maximum effectiveness near neutrality. Weaker binding to enzyme is generally displayed in either acidic or alkaline solution, with the position of the alkaline limb of the profiles depending on the pK(a) of the inhibitor. A reverse-protonation pattern of association with the enzyme is indicated, in which the hydroxamate anion of the inhibitor displaces a relatively acidic H(2)O ligand (pK(a) of 6) from the active-site zinc ion of carboxypeptidase A. The metal-coordinating, N-substituted hydroxamic acid functional groups exist in solution as a mixture of syn and anti rotamers, with relative abundances that depend on their pK(a). A pyrrolidinone analogue having a conformationally syn-fixed cyclohydroxamic acid was not an especially potent inhibitor. Structure-activity relationships suggest design criteria for hydroxamic acid inhibitors in order to provide most effective binding with metalloenzymes.