gebhart/patrepeval huggingface dataset:
Fusing Study of Silica-Polymer Composite Particles for Toners (2015)

Toner fusing is a critical step which determines both the quality of printed image and the amount of energy consumed by a laser printer. Therefore, manufacturers of laser printers and toners constantly work on improving fuser units and designing new toner formulations. It turns out that the external additives, which are primarily used to improve toner electrostatic charging, flowability, and durability, also affect toner fusing [1]. For instance, when used at relatively high loading treated silica affects toner melt flow behavior by increasing viscosity of melted toner. If viscosity of melted toner is high this results in inefficient flow of toner and poor fusing. Some toner manufacturers have begun using new external additives which enable fusability at low fusing temperatures. Silica composite particles - a new class of materials that was developed by Cabot Corp. for application in laser printer toners [2-4]. At the NIP29, we discussed how this material is synthesized and how it improves toner durability [5]. In this paper we demonstrate that the model toner formulations containing silica composite additives have better fusability than the formulations containing colloidal silica.
Resilience of mutual exclusion algorithms to transient memory faults (2011)

We study the behavior of mutual exclusion algorithms in the presence of unreliable shared memory subject to transient memory faults. It is well-known that classical 2-process mutual exclusion algorithms, such as Dekker and Peterson's algorithms, are not fault-tolerant; in this paper we ask what degree of fault tolerance can be achieved using the same restricted resources as Dekker and Peterson's algorithms, namely, three binary read/write registers.We show that if one memory fault can occur, it is not possible to guarantee both mutual exclusion and deadlock-freedom using three binary registers; this holds in general when fewer than 2f+1 binary registers are used and f may be faulty. Hence we focus on algorithms that guarantee (a) mutual exclusion and starvation-freedom in fault-free executions, and (b) only mutual exclusion in faulty executions. We show that using only three binary registers it is possible to design an 2-process mutual exclusion algorithm which tolerates a single memory fault in this manner. Further, by replacing one read/write register with a test&set register, we can guarantee mutual exclusion in executions where one variable experiences unboundedly many faults.In the more general setting where up to f registers may be faulty, we show that it is not possible to guarantee mutual exclusion using 2f + 1 binary read/write registers if each faulty register can exhibit unboundedly many faults. On the positive side, we show that an n-variable single-fault tolerant algorithm satisfying certain conditions can be transformed into an ((n-1)f + 1)-variable f-fault tolerant algorithm with the same progress guarantee as the original. In combination with our three-variable algorithm, this implies that there is a (2f+1)-variable mutual exclusion algorithm tolerating a single fault in up to f variables without violating mutual exclusion.
High-throughput Screening for Potent and Selective Inhibitors of Plasmodium falciparum Dihydroorotate Dehydrogenase (2005)

Plasmodium falciparum is the causative agent of the most serious and fatal malarial infections, and it has developed resistance to commonly employed chemotherapeutics. The de novo pyrimidine biosynthesis enzymes offer potential as targets for drug design, because, unlike the host, the parasite does not have pyrimidine salvage pathways. Dihydroorotate dehydrogenase (DHODH) is a flavin-dependent mitochondrial enzyme that catalyzes the fourth reaction in this essential pathway. Coenzyme Q (CoQ) is utilized as the oxidant. Potent and species-selective inhibitors of malarial DHODH were identified by high-throughput screening of a chemical library, which contained 220,000 drug-like molecules. These novel inhibitors represent a diverse range of chemical scaffolds, including a series of halogenated phenyl benzamide/naphthamides and urea-based compounds containing napthyl or quinolinyl substituents. Inhibitors in these classes with IC(50) values below 600 nm were purified by high pressure liquid chromatography, characterized by mass spectroscopy, and subjected to kinetic analysis against the parasite and human enzymes. The most active compound is a competitive inhibitor of CoQ with an IC(50) against malarial DHODH of 16 nm, and it is 12,500-fold less active against the human enzyme. Site-directed mutagenesis of residues in the CoQ-binding site significantly reduced inhibitor potency. The structural basis for the species selective enzyme inhibition is explained by the variable amino acid sequence in this binding site, making DHODH a particularly strong candidate for the development of new anti-malarial compounds.
Automatic synthesis of composable sequential quantum Boolean circuits (2006)

This paper presents a methodology to transfer self-timed circuit specifications into sequential quantum Boolean circuits (SQBCs) and composable SQBCs (CQBCs). State graphs (SGs) are used to describe the behaviors of self-timed circuits and then are translated into SQBCs based on Toffoli gates. The concept of IP (intellectual property) reuse is applied to the constructed SQBCs to produce reusable and composable quantum Boolean circuits (CQBCs). Therefore, these reusable CQBCs as basic modular components can be exploited to construct more complicated quantum Boolean circuits. A set of self-timed components is successfully and automatically synthesized into CQBCs by our methodology. These CQBCs can be used as building blocks to compose control-path components of self-timed systems.
Oocyst-Induced Toxoplasma gondii Infections in Cats (1989)

To investigate the oocyst-induced cycle with a 21+ day prepatent period, 32 cats were fed 5 x 10(5) to 2 x 10(7) sporulated oocysts of Toxoplasma gondii and necropsied between 4 hr and 41 days thereafter. The presence of the earliest stages in 7 cats was tested in mice. The tissues of 25 cats were studied histologically; 17 were bioassayed by feeding them to cats to determine, by the length of the prepatent period, whether bradyzoites were present. Based on previous studies, a short (3-10 days) prepatent period indicated that bradyzoites were present in an oral inoculum and a long (greater than 21 days) prepatent period indicated the presence of tachyzoites only. Tissues from 14 cats were also bioassayed in mice for the presence of bradyzoites, using their resistance to pepsin as indicator. Six were studied by both methods. Based on these criteria, tachyzoites predominated in extraintestinal organs during the first 14 days after infection. They were found as early as 4 hr in mesenteric lymph nodes where their number reached 10(4) after 6 and 9 days; they were present after 1 day in all levels of the small intestine and after 6 days in the liver, lung, and blood. Bradyzoites were first detected 10 days after oocyst feeding; they predominated by the third week of infection and were present up to 41 days. Enteroepithelial stages were found histologically only in 2 cats, 24 and 41 days after inoculation.(ABSTRACT TRUNCATED AT 250 WORDS)
High-pressure cooling of protein crystals without cryoprotectants (2005)

Flash-cooling of protein crystals is the best known method to effectively mitigate radiation damage in macromolecular crystallography. To prevent physical damage to crystals upon cooling, suitable cryoprotectants must usually be found, a process that is time-consuming and in some cases unsuccessful. A method is described to cool protein crystals in high-pressure helium gas without the need for penetrative cryoprotectants. The method involves mounting protein crystals from the native mother liquor in a cryoloop with a droplet of oil, pressurizing the crystal to 200 MPa in He gas, cooling the crystal under pressure and then releasing the pressure. The crystal is then removed from the apparatus under liquid nitrogen and handled thereafter like a normal cryocooled crystal. Results are presented from three representative proteins. Dramatic improvement in diffraction quality in terms of resolution and mosaicity was observed in all cases. A mechanism for the pressure cooling is proposed involving high-density amorphous (HDA) ice which is produced at high pressure and is metastable at room pressure and 110 K.
Spintronic/CMOS-Based Thermal Sensors (2020)

Stacking more systems into a compact area or scaling devices to increase the density of integration are two approaches to provide greater functional complexity. Excessive heat generated as a result of these technology advancements leads to an increase in leakage power and degradation in system reliability. Hence, a thermal aware system composed of hundreds of distributed thermal sensor nodes is needed. Such a system requires an efficient thermal sensor placed close to the thermal hotspots, small in size, fast response, and CMOS compatibility. In this paper, two hybrid spintronic/CMOS circuits are proposed. These circuits exhibit a low power consumption of 11.9 μW during the on-state, a linearity (R <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> ) of 0.96 over the industrial temperature range of operation (-40 to 125) <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">o</sup> C, and a sensitivity of 3.78 mV/K.
Construction and modification of 3D geometry using a sketch-based interface (2006)

We present an interactive pen-based computer program for designing 3D objects through direct sketching. The proposed techniques are tailored toward the creation of free-form curves and surfaces, and are therefore particulary useful for styling design purposes. In our approach, the design process consists of two main steps. In the first step, the user designs a wireframe model by sketching its constituent curves in 3D. Using purely sketch-based operations, the initial curves can then be modified as desired. In the second step, the user constructs interpolating surfaces on the wireframe to obtain a solid model. Again, through sketch-based operations, the user can modify the initial surfaces, and specify the boundary conditions if necessary. In addition to the main modeling operations, a gesture-based command interface allows many of the frequently used commands to be input through pen strokes. The utility of our system is demonstrated with various examples.
Isolation of a Bacterium Capable of Degrading Peanut Hull Lignin (1983)

Thirty-seven bacterial strains capable of degrading peanut hull lignin were isolated by using four types of lignin preparations and hot-water-extracted peanut hulls. One of the isolates, tentatively identified as Arthrobacter sp., was capable of utilizing all four lignin preparations as well as extracted peanut hulls as a sole source of carbon. The bacterium was also capable of degrading specifically labeled [C]lignin-labeled lignocellulose and [C]cellulose-labeled lignocellulose from the cordgrass Spartina alterniflora and could also degrade [C]Kraft lignin from slash pine. After 10 days of incubation with [C]cellulose-labeled lignocellulose or [C]lignin-labeled lignocellulose from S. alterniflora, the bacterium mineralized 6.5% of the polysaccharide component and 2.9% of the lignin component.
DMAC and NMP as Electrolyte Additives for Li-Ion Cells (2008)

Dimethyl acetamide (DMAC) and N-methyl pyrrolidinone (NMP) have been found to be useful as high-temperature-resilience-enhancing additives to a baseline electrolyte used in rechargeable lithium-ion electrochemical cells. The baseline electrolyte, which was previously formulated to improve low-temperature performance, comprises LiPF6 dissolved at a concentration of 1.0 M in a mixture comprising equal volume proportions of ethylene carbonate, diethyl carbonate, and dimethyl carbonate. This and other electrolytes comprising lithium salts dissolved in mixtures of esters (including alkyl carbonates) have been studied in continuing research directed toward extending the lower limits of operating temperatures and, more recently, enhancing the of such cells. This research at earlier stages, and the underlying physical and chemical principles, were reported in numerous previous NASA Tech Briefs articles. Although these electrolytes provide excellent performance at low temperatures (typically as low as -40 C), when the affected Li-ion cells are subjected to high temperatures during storage and cycling, there occur irreversible losses of capacity accompanied by power fade and deterioration of low-temperature performance. The term high-temperature resilience signifies, loosely, the ability of a cell to resist such deterioration, retaining as much as possible of its initial charge/discharge capacity during operation or during storage in the fully charged condition at high temperature. For the purposes of the present development, a temperature is considered to be high if it equals or exceeds the upper limit (typically, 30 C) of the operating-temperature range for which the cells in question are generally designed.
Chromosome-End Knockoff Strategy to Reshape Alkaloid Profiles of a Fungal Endophyte (2016)

Molecular genetic techniques to precisely eliminate genes in asexual filamentous fungi require the introduction of a marker gene into the target genome. We developed a novel strategy to eliminate genes or gene clusters located in subterminal regions of chromosomes, and then eliminate the marker gene and vector backbone used in the transformation procedure. Because many toxin gene clusters are subterminal, this method is particularly suited to generating nontoxic fungal strains. We tested this technique on Epichloë coenophiala, a seed-transmissible symbiotic fungus (endophyte) of the important forage grass, tall fescue (Lolium arundinaceum). The endophyte is necessary for maximal productivity and sustainability of this grass but can produce ergot alkaloids such as ergovaline, which are toxic to livestock. The genome sequence of E. coenophiala strain e19 revealed two paralogous ergot alkaloid biosynthesis gene clusters, designated EAS1 and EAS2. EAS1 was apparently subterminal, and the lpsB copy in EAS2 had a frame-shift mutation. We designed a vector with a fungal-active hygromycin phosphotransferase gene (hph), an lpsA1 gene fragment for homologous recombination at the telomere-distal end of EAS1, and a telomere repeat array positioned to drive spontaneous loss of hph and other vector sequences, and to stabilize the new chromosome end. We transformed E. coenophiala with this vector, then selected "knockoff" endophyte strains, confirmed by genome sequencing to lack 162 kb of a chromosome end including most of EAS1, and also to lack vector sequences. These ∆EAS1 knockoff strains produced no detectable ergovaline, whereas complementation with functional lpsB restored ergovaline production.
Speech synthesizer and method for synthesizing speech for superposing and adding a waveform onto a waveform obtained by delaying a previously obtained waveform (1995)

First Page
Sialidase Activity of Influenza A Virus in an Endocytic Pathway Enhances Viral Replication (2005)

N2 neuraminidase (NA) genes of the 1957 and 1968 pandemic influenza virus strains possessed avian-like low-pH stability of sialidase activity, unlike most epidemic strains. We generated four reverse-genetics viruses from a genetic background of A/WSN/33 (H1N1) that included parental N2 NAs of 1968 pandemic (H3N2) and epidemic (H2N2) strains or their counterpart N2 NAs in which the low-pH stability of the sialidase activity was changed by substitutions of one or two amino acid residues. We found that the transfectant viruses bearing low-pH-stable sialidase (WSN/Stable-NAs) showed 25- to 80-times-greater ability to replicate in Madin-Darby canine kidney (MDCK) cells than did the transfectant viruses bearing low-pH-unstable sialidase (WSN/Unstable-NAs). Enzymatic activities of WSN/Stable-NAs were detected in endosomes of MDCK cells after 90 min of virus internalization by in situ fluorescent detection with 5-bromo-4-chloro-indole-3-yl-alpha-N-acetylneuraminic acid and Fast Red Violet LB. Inhibition of sialidase activity of WSN/Stable-NAs on the endocytic pathway by pretreatment with 4-guanidino-2,4-dideoxy-N-acetylneuraminic acid (zanamivir) resulted in a significant decrease in progeny viruses. In contrast, the enzymatic activities of WSN/Unstable-NAs, the replication of which had no effect on pretreatment with zanamivir, were undetectable in cells under the same conditions. Hemadsorption assays of transfectant-virus-infected cells revealed that the low-pH stability of the sialidase had no effect on the process of removal of sialic acid from hemagglutinin in the Golgi regions. Moreover, high titers of viruses were recovered from the lungs of mice infected with WSN/Stable-NAs on day 3 after intranasal inoculation, but WSN/Unstable-NAs were cleared from the lungs of the mice. These results indicate that sialidase activity in late endosome/lysosome traffic enhances influenza A virus replication.
LEU3 of <i>Saccharomyces cerevisiae</i> Activates Multiple Genes for Branched-Chain Amino Acid Biosynthesis by Binding to a Common Decanucleotide Core Sequence (1988)

LEU3 of Saccharomyces cerevisiae encodes an 886-amino-acid polypeptide that regulates transcription of a group of genes involved in leucine biosynthesis and has been shown to bind specifically to a 114-base-pair DNA fragment of the LEU2 upstream region (P. Friden and P. Schimmel, Mol. Cell. Biol. 7:2707-2717, 1987). We show here that, in addition to LEU2, LEU3 binds in vitro to sequences in the promoter regions of LEU1, LEU4, ILV2, and, by inference, ILV5. The largely conserved decanucleotide core sequence shared by the binding sites in these genes is CCGGNNCCGG. Methylation interference footprinting experiments show that LEU3 makes symmetrical contacts with the conserved bases that lie in the major groove. Synthetic oligonucleotides (19 to 29 base pairs) which contain the core decanucleotide and flanking sequences of LEU1, LEU2, LEU4, and ILV2 have individually been placed upstream of a LEU3-insensitive test promoter. The expression of each construction is activated by LEU3, although the degree of activation varies considerably according to the specific oligonucleotide which is introduced. A promoter construction with substitutions in the core sequence remains LEU3 insensitive, however. One of the oligonucleotides (based on a LEU2 sequence) was also tested and shown to confer leucine-sensitive expression on the test promoter. The results demonstrate that only a short sequence element is necessary for LEU3-dependent promoter binding and activation and provide direct evidence for an expanded repertoire of genes that are activated by LEU3.
<i>N</i>-Propionylated Group B Meningococcal Polysaccharide Mimics a Unique Bactericidal Capsular Epitope in Group B <i>Neisseria meningitidis</i> (1997)

The N-propionylated group B meningococcal polysaccharide (NPrGBMP) mimics a unique protective epitope on the surface of group B meningococci (GBM) and Escherichia coli K1. Using a series of monoclonal antibodies (mAbs) induced by the NPrGBMP-monomeric tetanus toxoid (TT) conjugate vaccine it was demonstrated that mAbs having specificities for both extended and conventional short segments of the NPrGBMP were formed, but only the former were bactericidal, and/or gave passive protection against live challenge by GBM. The failure of mAbs specific for short epitopes to protect was further established when (NeuPr)4-TT was used as the vaccine. Of all the mAbs produced that were specific for short internal segments of the NPrGBMP, none were protective, despite the fact that most of them cross-react with the GBM capsular polysaccharide. In contrast, most of the protective mAbs produced by NPrGBMP- TT did not recognize the group B meningococcal polysaccharide (GBMP) unless it was present in its aggregated high molecular weight form. The bactericidal epitope mimicked by the NPrGBMP was shown to be ubiquitous in the capsule of both GBM and E. coli K1 using immunogold labeling techniques and, because of its unique properties, its identification could be significant in the development of a comprehensive conjugate vaccine against group B meningococcal meningitis. This is because most known human alpha(2-8)-polysialic acid self-antigens can be accommodated in 30-50 alpha(2-8)-linked sialic acid residues, which is roughly equivalent to an 11-kD length of the GBMP. It has been hypothesized that the formation of the protective epitope on the surface of GBM is due to the interaction of helical segments of the GBMP with another molecule and that the protective epitope is mimicked by the NPrGBMP. Support for the above hypothesis is provided by the fact that the protective NPrGBMP epitope has a similar unusual length dependency to that of the GBMP epitope.
Hydrogel Nanosensors for Colorimetric Detection and Dosimetry in Proton Beam Radiotherapy (2018)

Proton beam therapy (PBT) is a state-of-the-art radiotherapy treatment approach that uses focused proton beams for tumor ablation. A key advantage of this approach over conventional photon radiotherapy (XRT) is the unique dose deposition characteristic of protons, which results in superior healthy tissue sparing. This results in fewer unwanted side effects and improved outcomes for patients. Currently available dosimeters are intrinsic, complex, and expensive and are not routinely used to determine the dose delivered to the tumor. Here, we report a hydrogel-based plasmonic nanosensor for detecting clinical doses used in conventional and hyperfractionated proton beam radiotherapy. In this nanosensor, gold ions, encapsulated in a hydrogel, are reduced to gold nanoparticles following irradiation with proton beams. Formation of gold nanoparticles renders a color change to the originally colorless hydrogel. The intensity of the color can be used to calibrate the hydrogel nanosensor in order to quantify different radiation doses employed during proton treatment. The potential of this nanosensor for clinical translation was demonstrated using an anthropomorphic phantom mimicking a clinical radiotherapy session. The simplicity of fabrication, detection range in the fractionated radiotherapy regime, and ease of detection with translational potential makes this a first-in-kind plasmonic colorimetric nanosensor for applications in clinical proton beam therapy.
The formation of ultrafine ferrite in low C steels through thermomechanical processing (2001)

\n\t\t\t\t\tUltrafine ferrite can be formed in steels through relatively simple thermomechanical processes. The ferrite nucleates intragranularly within the austenite grain on deformation features, which are favoured by heavy shear and large effective strains. It is also possible to produce ultrafine microstructures under multipass deformation conditions, although these may be due to dynamic recovery rather than strain induced transformation.<br />\n\t\t\t\t
Activated Pyridinium‐Tagged Ruthenium Complexes as Efficient Catalysts for Ring‐Closing Metathesis. (2007)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
An adaptive connectivity restoration algorithm for wireless sensor and actor networks (2013)

Wireless Sensor and Actor Networks (WSANs) depend heavily on connectivity among actors to perform their tasks. Loss of strong connectivity caused by the failure of an actor may partition the deployed WSAN and degrade its functionality. In this paper, we present an Adaptive Connectivity Restoration Approach (ACRA), which works autonomously and enables the network to self-heal and restore connectivity. ACRA is an on-demand approach that does not impose any overhead prior to the failure. The mathematical analysis and simulation results show that ACRA has a low cost in both large and small networks and thus, suits dense and sparse deployments.