gebhart/patrepeval huggingface dataset:
Lithium chloride effectively kills the honey bee parasite Varroa destructor by a systemic mode of action (2018)

Honey bees are increasingly important in the pollination of crops and wild plants. Recent reports of the weakening and periodical high losses of managed honey bee colonies have alarmed beekeeper, farmers and scientists. Infestations with the ectoparasitic mite Varroa destructor in combination with its associated viruses have been identified as a crucial driver of these health problems. Although yearly treatments are required to prevent collapses of honey bee colonies, the number of effective acaricides is small and no new active compounds have been registered in the past 25 years. RNAi-based methods were proposed recently as a promising new tool. However, the application of these methods according to published protocols has led to a surprising discovery. Here, we show that the lithium chloride that was used to precipitate RNA and other lithium compounds is highly effective at killing Varroa mites when fed to host bees at low millimolar concentrations. Experiments with caged bees and brood-free artificial swarms consisting of a queen and several thousand bees clearly demonstrate the potential of lithium as miticidal agent with good tolerability in worker bees providing a promising basis for the development of an effective and easy-to-apply control method for mite treatment.
Fully Biodegradable Magnetic Micro-Nanoparticles: A New Platform for Tissue Regeneration and Theranostic (2013)

Nowadays, magnetic materials are receiving special attention due to their potential applications in different fields and in particular in medicine. Magnetic micro-nano-particles have been progressively employed as support materials for enzyme immobilization, and have been used as drug-delivery vehicles, contrast agents for magnetic resonance imaging as well as heat mediators for hyperthermia-based anti-cancer treatments and many other exciting biomedical applications. Magnetic materials have also attracted a big interest in the field of bone tissue regeneration because it has been demonstrated that magnetic nanoparticles have effect of osteoinduction even without external magnetic force. Therefore, one of the most big challenge in this field is the production of magnetic materials with good biocompatibility and biodegradability. In fact, the long-term effects in the human body of iron oxide (maghemite or magnetite), the most popular magnetic phase used in medicine and biotechnology, are not yet completely assessed. To this aim, in this work we developed an innovative biocompatible and bioresorbable superparamagnetic-like phase by doping nano-hydroxyapatite with Fe2+/Fe3+ ions (FeHA). Moreover the same magnetic nanoparticles were used as nano-particulate emulsifier for the preparation of hollow hybrid Fe-HA-poly(L-lactic) acid (PLLA) micro-nano-spheres. PLLA has been used because poly(α-hydroxy-esters) are the most frequently used synthetic polymers for biomedical applications owing to their biocompatibility, hydrolytic degradation process and proper mechanical properties. These micro-nanospheres could be used as new type of scaffold for hard tissue regeneration. In fact, spherical scaffold display several advantages respect to the monolithic counterpart e.g., (i) improving control over sustained delivery of therapeutic agents, signalling biomolecules and even pluripotent stem cells, (ii) serving as stimulus-sensitive delivery vehicles for triggered release, (iii) introducing porosity and/or improve the mechanical properties of bulk scaffolds by acting as porogen or reinforcement phase, (iv) supplying compartmentalized micro-reactors for dedicated biochemical processes, (v) functioning as cell delivery vehicle, and, finally, (vi) giving possibility of preparing injectable and/or mouldable formulations to be applied by using minimally invasive surgery. Moreover, the same magnetic materials could find applications in nanomedicine as a multifunctional carrier. Their magnetic functionality could be utilized to move them into the body towards target organs by an external magnetic field. Furthermore, the superparamagnetic feature of the nanoparticles could allow to tailor the release of the therapeutic agent by switching (on-off) the external magnetic field and/or to treat cancer cells by hyperthermia.
Inheritance of Elevated Palmitic Acid Content in Soybean Seed Oil (1991)

Soybean [ Glycine max (L.) Merr.] oil with an elevated palmitic acid content may be useful for production of some food and industrial products. Two mutant soybean lines with palmitic acid contents of > 180 g kg ‐1 were developed by treatment of ‘A1937’ seeds with N ‐nitroso‐ N ‐methylurea (NMU) and ‘Elgin’ seeds with ethyl methanesuifonate (EMS). The mutant lines, A1937NMU‐85 and EiginEMS‐421, were crossed to determine their genetic relationship for elevated paimitic acid content. The F 2 and F 3 generations were grown in the field and transgressive segregates with >250 g kg ‐1 palmitic acid were obtained, the highest content known in soybean. The segregation ratios indicate that the two mutant lines have different alleles at two independent loci. A1937NMU‐85 was crossed to C1727, a line with the fap2 allele that controls increased palmitic acid content. The lack of transgressive segregation in the F 2 generation of the cross indicate that AI937NMU‐85 and C1727 have alleles controlling high palmitic acid at the same locus or at tightly linked loci. The allele in AI937NMU‐85 was designated fap2‐b . The allele in ElginEMS‐421 was given the temporary designation of fap ?(ElginEMS‐421) until its relationship to other alleles for modified palmitic acid content can be determined.
Design and operation of a variable temperature scanning tunnelling microscope (1988)

SUMMARY A new variable temperature STM has been developed which utilizes two concentric piezoelectric tubes; an inner scanning tube, and an outer thermal compensation tube which also provides for inertial translation of the sample into tunnelling range. With this design, continuously variable temperature operation is demonstrated for the first time in an STM. Also, by eliminating all mechanical components such as springs, levers and gears, which normally couple directly to the tunnelling gap in other designs, atomic resolution operation is demonstrated in which no vibration isolation is necessary. During operation, the inside of the scanning tube is maintained at ground potential while the feedback signal is electronically summed to the scanning voltages applied to the outer quadrants. In addition to shielding the sensitive tunnelling circuit, this mode of operation enables one to electronically balance out mechanical imperfections of the scanning tube. To date, this new STM has been operated over the 77–400 K temperature range with the observed thermal drift as low as 1 Å/h and 10 Å/K. Another useful feature of this new design is the ability to reposition a sample to within 200 Å of the same location after it has been translated macroscopic distances (several mm) out of tunnelling range.
Purification of Aggressive Supercritical Natural Gas Using Carbon Molecular Sieve Hollow Fiber Membranes (2017)

In this paper, we describe polyimide-derived carbon molecular sieve (CMS) hollow fiber membranes with CO2/CH4 separation factors ∼60 under a supercritical (1800 psia) natural gas feed comprising 50% CO2 and 500 ppm highly condensable C7 hydrocarbons. Long-term tests extending for 200 h proved membrane stability. Temperatures ranging from −50 to 100 °C were also tested, and the membrane showed attractive performance under the diverse conditions studied. With attractive and stable separation performance, the CMS hollow fiber membranes studied in this work can potentially enable next-generation CO2 removal processes for challenging natural gas feeds.
Roller Bottle Culture, Mixing (1999)

Introduction Background Development and Experiment Validation of a Computational Model of Fluid and Cell Motion in a Roller Bottle Flow Field Solution The Fluid Velocity Field in a Roller Bottle The Motion of Cells in a Roller Bottle Detailed Analysis of Cell Settling Particle Settling Results Effects of Bottle Rotation Speed on Cell Culture Experiments Improving the Performance of Roller Bottles Using Unsteady Rotation to Enhance Settling Using Rocking Motion to Enhance Axial Mixing Conclusions Bibliography
Defective interfering influenza virus confers only short-lived protection against influenza virus disease: Evidence for a role for adaptive immunity in DI virus-mediated protection in vivo (2011)

We have shown earlier that a single dose of cloned defective interfering (DI) influenza A virus strongly protects mice from disease following a lethal challenge with different subtypes of influenza A virus. These animals suffered no clinical disease but experienced a subclinical infection which rendered them immune to reinfection with the same challenge virus. However, little is known about how DI virus achieves such protection. Here we investigated the role of adaptive immunity in DI virus-mediated protection using severe-combined immunodeficient (SCID) mice, which lack competence in both B- and T-cell compartments but retain NK cell activity. SCID mice which were treated with DI virus and infected with influenza virus initially remained completely well, while infected litter mates that received UV-inactivated DI virus became seriously ill and died. However, after 10 days of good health, the DI virus-protected SCID mice developed a clinical disease that was similar, but not completely identical, to the acute influenza disease. Disease was delayed longer by a higher dose of DI virus. We excluded the possibilities that the DI virus load in the lungs had declined, that the DI RNA sequence had changed so that it no longer interfered with the infectious genome, or that infectious virus had become resistant to the DI virus. These data show that while DI virus provides full protection from the acute disease in the absence of adaptive immunity, that same immunity is essential for clearing the infection. This indicates that the conventional view that DI virus-induced protection is mediated solely by competition for replication with the challenge virus is incorrect for influenza virus.
Alternative proteolytic processing of platelet membrane glycoprotein IIb. (1988)

Platelet membrane glycoprotein (GP) IIb-IIIa is a component of a receptor for the adhesive proteins fibrinogen, fibronectin, and von Willebrand factor. GPIIb is initially synthesized as a single-chain polypeptide that is proteolytically processed to yield the two chains of mature GPIIb present on the cell surface. Analysis of the amino acid sequence surrounding the proposed light-heavy chain junction of GPIIb suggests a second potential site following a pair of basic residues 12-15 residues upstream from the reported amino terminus of the light chain. We have utilized anti-peptide antibodies to examine the possibility of alternative cleavage at these two potential sites. Peptide V43 precedes the dibasic sequence and is known to reside in the heavy chain. Peptide V41 contains the sequence between the two potential sites. In immunoblots, anti-V43 reacted only with the heavy chain while anti-V41 reacted only with the light chain. Immunoprecipitation of surface-labeled platelets indicated 97% of the GPIIb light chain contains the V41 sequence while approximately 3% of GPIIb molecules lack the V41 sequence on both the light and heavy chains. These data indicate that GPIIb is primarily cleaved 12-15 amino acids upstream from the reported amino terminus of the light chain while in a minor proportion of GPIIb molecules cleavage occurs at both sites.
Abstract 2528: 3C23K: an anti-human Mullerian inhibiting substance type II receptor humanized monoclonal antibody for ovarian cancer targeted therapy (2012)

Abstract Asymptomatic in early stages, ovarian cancer is a “silent killer” representing the fifth leading cause of female deaths in western countries. Every year 56,967 women in Europe and USA die as a consequence of this disease. The incidence of ovarian cancer is forecast to undergo a 13 % increase in the next eight years in the seven major developed countries to reach about 72,000 annual cases in 2019. Due to the limitations of the current therapeutic approaches, there is a strong need for novel, more efficient, therapies. For this reason, we have produced a humanized monoclonal antibody 3C23K targeting the human Müllerian Inhibiting Substance type II Receptor (MISRII), expressed on most ovarian cancer subtypes, including epithelial ovarian cancer (EOC) representing more than 90% of ovarian cancers. This monoclonal antibody derives from the murine monoclonal antibody 12G4 and displays a particular glycosylation profile known to favor effector recruitment (EMABling®) as previously demonstrated in vitro. In vivo, we also showed that 3C23K exhibited a significant effect on tumor growth against several ovarian tumor xenografted models derived from patient primary EOC tumors. In this study, we first confirmed by peptide microarray that the epitope of 3C23K antibody was strictly identical to that of 12G4 antibody and a 3D-model of the full MISRII molecule was generated in order to better localize the 3C23K epitope. Furthermore, SPR studies demonstrated cross-reactivity of 3C23K with MISRII of rabbit, dog, pig, cow and primate. In vivo, antitumor activity of 3C23K against xenografted EOC tumor models was confirmed with various concentrations and treatment schedules. Moreover, interestingly, we constructed a mutant form of 3C23K harboring two mutations in the Fc region (G236R/L328R) in order to prevent binding to both murine and human FcαRs, and demonstrated that such a modification abolished antitumor activity. This data confirmed in vivo that recruitment of effectors is essential for 3C23K efficacy. Finally, in order to anticipate clinical treatment of ovarian cancers, 3C23K was tested in vivo in combination with carboplatin. Combination, when compared with treatment of each product alone, resulted in more than additive antitumor activity. Altogether these data showed that humanized monoclonal antibody 3C23K represents a promising candidate for ovarian cancer immunotherapy. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 2528. doi:1538-7445.AM2012-2528
Cinchonium Betaines as Efficient Catalysts for Asymmetric Proton Transfer Catalysis: The Development of a Practical Enantioselective Isomerization of Trifluoromethyl Imines (2016)

We have developed a new class of cinchonium betaine catalysts bearing both a base moiety and an aromatic moiety as an N-substituent of the quinuclidine motif. These cinchonium betaines were found to promote proton transfer catalysis with 1000-5000 turnovers per 24 h, thereby enabling us to realize highly efficient enantioselective isomerization of trifluoromethyl imines to provide a practical access to optically active trifluoromethylated amines.
Gas perfusable microfabricated membranes for high-density cell culture (2017)

We report for the first time the design, fabrication, and feasibility of a high-density cell culture system capable of a cell substrate density of 40 cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> /cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sup> , greater than 250× current commercially available systems. This design provides oxygen directly from gas perfusable membranes on which the cells are grown to overcome oxygen delivery limitations in traditional culture systems. With translation in mind, the membranes are composed only of medical grade PDMS and Parylene to mitigate contaminant leaching concerns.
ChemInform Abstract: Synthesis and Reactivity of Olefin Metathesis Catalysts Bearing Cyclic (Alkyl)(Amino)Carbenes. (2007)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Advanced Combustor Concepts for Low Emissions Supersonic Propulsion (2012)

Novel lean-burn combustor concepts were designed and evaluated for supersonic aircraft propulsion, with a focus on cruise NOx emissions. Premixing to lean conditions is especially challenging at supersonic cruise because combustor inlet temperatures are high and autoignition times are short. However, combustor pressure is significantly lower than at takeoff, so at cruise this allows heated jet fuel to be vaporized before injection as an aid to mixing. Two concepts — differentiated by swirler aerodynamics, swirler size, and staging method — were evaluated in the work reported here, both using injection of vaporized jet fuel. CFD calculations of mixing and combustion were used to design hardware for each concept. Injectors for each were fabricated using stereolithography (SLA) for cold-flow mixing tests, and using metal fabrication for subsequent combustion tests. Combustion test results show that EINOx < 5 was achieved for both concepts in single-sector tests at supersonic cruise combustor conditions.
Synthesis of C‐4 Substituted Nicotine Derivatives via an N‐Acylpyridinium Salt of (S)‐Nicotine. (2006)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
UML-Based integration testing (2000)

Increasing numbers of software developers are using the Unified Modeling Language (UML) and associated visual modeling tools as a basis for the design and implementation of their distributed, component-based applications. At the same time, it is necessary to test these components, especially during unit and integration testing. At Siemens Corporate Research, we have addressed the issue of testing components by integrating test generation and test execution technology with commercial UML modeling tools such as Rational Rose; the goal being a design-based testing environment. In order to generate test cases automatically, developers first define the dynamic behavior of their components via UML Statecharts, specify the interactions amongst them and finally annotate them with test requirements. Test cases are then derived from these annotated Statecharts using our test generation engine and executed with the help of our test execution tool. The latter tool was developed specifically for interfacing to components based on COM/DCOM and CORBA middleware.
The RAD51-Stimulatory Compound RS-1 Can Exploit the RAD51 Overexpression That Exists in Cancer Cells and Tumors (2014)

RAD51 is the central protein that catalyzes DNA repair via homologous recombination, a process that ensures genomic stability. RAD51 protein is commonly expressed at high levels in cancer cells relative to their noncancerous precursors. High levels of RAD51 expression can lead to the formation of genotoxic RAD51 protein complexes on undamaged chromatin. We developed a therapeutic approach that exploits this potentially toxic feature of malignancy, using compounds that stimulate the DNA-binding activity of RAD51 to promote cancer cell death. A panel of immortalized cell lines was challenged with the RAD51-stimulatory compound RS-1. Resistance to RS-1 tended to occur in cells with higher levels of RAD54L and RAD54B, which are Swi2/Snf2-related translocases known to dissociate RAD51 filaments from dsDNA. In PC3 prostate cancer cells, RS-1-induced lethality was accompanied by the formation of microscopically visible RAD51 nuclear protein foci occurring in the absence of any DNA-damaging treatment. Treatment with RS-1 promoted significant antitumor responses in a mouse model, providing proof-of-principle for this novel therapeutic strategy.
Antitumor immune response and interleukin 2 production induced in colorectal cancer patients by immunization with human monoclonal anti-idiotypic antibody. (1991)

The immunogenicity of human anti-idiotypic antibody has been investigated using a human monoclonal anti-idiotypic antibody (105AD7) which interacts with the binding site of 791T/36, a mouse monoclonal antibody against gp72 antigen. This antigen is frequently expressed in gastrointestinal cancer, therefore, six patients with advanced colorectal cancer have been immunized with 105AD7 as an aluminum hydroxide gel precipitate in a phase I clinical study. Cryopreserved blood mononuclear cells were tested for in vitro proliferative responses by [3H]thymidine incorporation; plasma samples were tested by enzyme-linked immunosorbent assay for anti-anti-idiotypic and antitumor antibodies, and for interleukin 2. Proliferative responses to gp72 positive tumor cells were seen in four of five patients tested; parallel in vitro responses to 105AD7 anti-idiotypic antibody were seen in most of these patients. Interleukin 2 was detected in the plasma of four of six patients after 105AD7 immunization, with peak levels up to 7 units/ml. No toxicity related to anti-idiotype immunization and no antitumor or anti-anti-idiotype antibodies were seen. This study shows that human monoclonal anti-idiotype 105AD7 is immunogenic in cancer patients, inducing cellular antitumor responses and interleukin 2 production. This suggests that human monoclonal anti-idiotype antibodies may have considerable potential for immunotherapy of human cancer.
Convergent insights into mechanisms determining disease and resistance response in plant–fungal interactions (1995)

In this review, an attempt has been made to scrutinize mechanisms controlling disease or resistance from the perspective of biological forces and constraints affecting co-evolution of a fungal pathogen with its plant host. We reckon that plants are naturally resistant to almost all potentially pathogenic organisms. Defense mechanisms responsible for this default resistance, also termed nonhost or general resistance, are many and include both physical and chemical factors triggered rapidly in response to attempted infection. Pathogenic organisms have to contend with these mechanisms before they can succeed in colonizing a plant. It appears that two different strategies, biotrophic and necrotrophic, have evolved in fungi for this purpose. In the former, defenses are not allowed to be triggered, and in the latter these mechanisms are suppressed or nullified. Consequently, two different kinds of resistance mechanisms have evolved in plants. Against biotrophs, resistance genes function to ensure that normal plant defenses are triggered in time to keep the plant resistant to the pathogen. Against necrotrophs, resistance genes operate to negate the key pathogenic strategy of the invader. Further evolution between the host and the pathogen, and hence durability of a disease resistance gene, is governed by the importance of the fungal target of a disease resistance gene in plant pathogenesis. Key words: disease resistance, plant–fungal interaction, plant pathogenesis, gene-for-gene interaction, defense mechanisms.