gebhart/patrepeval huggingface dataset:
Phased tag cache: an efficient low power cache system (2004)

In this paper, we propose a low power cache design, namely phased tag cache, for reducing the power consumption of set-associative caches. In the phased tag cache, the tag is compared in two phases. A small part of the tag is compared in the first phase to determine the data way which a memory reference falls into. The remaining bits of the tag are compared in the second phase to verify if the result from the first phase is valid. By doing so we can eliminate most of the unnecessary activities on the entire tag. We used the CACTI cache model to show that the time overhead of the phased tag cache is small. Simulation results based on Spec2000 benchmark applications suggest that the phased tag cache design has small impact on the cache performance. The power model shows the phased tag design reduces the power consumption by 30-50%, compared to conventional caches used by the Itanium2 processor.
AKT and AMP-activated protein kinase regulate TBC1D1 through phosphorylation and its interaction with the cytosolic tail of insulin-regulated aminopeptidase IRAP (2018)

In skeletal muscle, the Rab GTPase-activating (GAP) protein TBC1D1 is phosphorylated by AKT and AMP-activated protein kinase (AMPK) in response to insulin and muscle contraction. Genetic ablation of <i>Tbc1d1</i> or mutation of distinct phosphorylation sites impairs intracellular GLUT4 retention and GLUT4 traffic, presumably through alterations of the activation state of downstream Rab GTPases. Previous studies have focused on characterizing the C-terminal GAP domain of TBC1D1 that lacks the known phosphorylation sites, as well as putative regulatory domains. As a result, it has been unclear how phosphorylation of TBC1D1 would regulate its activity. In the present study, we have expressed, purified, and characterized recombinant full-length TBC1D1 in <i>Sf9</i> insect cells via the baculovirus system. Full-length TBC1D1 showed RabGAP activity toward GLUT4-associated Rab8a, Rab10, and Rab14, indicating similar substrate specificity as the truncated GAP domain. However, the catalytic activity of the full-length TBC1D1 was markedly higher than that of the GAP domain. Although <i>in vitro</i> phosphorylation of TBC1D1 by AKT or AMPK increased 14-3-3 binding, it did not alter the intrinsic RabGAP activity. However, we found that TBC1D1 interacts through its N-terminal PTB domains with the cytoplasmic domain of the insulin-regulated aminopeptidase, a resident protein of GLUT4 storage vesicles, and this binding is disrupted by phosphorylation of TBC1D1 by AKT or AMPK. In summary, our findings suggest that other regions outside the GAP domain may contribute to the catalytic activity of TBC1D1. Moreover, our data indicate that recruitment of TBC1D1 to GLUT4-containing vesicles and not its GAP activity is regulated by insulin and contraction-mediated phosphorylation.
High performance gate drives for utilizing the IGBT in the active region (2002)

The advantages of operating the IGBT in its active region for active snubbing and series operation have been established in the literature. In this paper we describe the implementation and performance of several gate drive circuits suitable for use in closed loop control of the IGBT voltage. The application of the gate drives to active control of a wide range of IGBT devices is demonstrated and discussed.
Adenoviral transfer of HSP-70 into pulmonary epithelium ameliorates experimental acute respiratory distress syndrome (2002)

The acute respiratory distress syndrome (ARDS) provokes three pathologic processes: unchecked inflammation, interstitial/alveolar protein accumulation, and destruction of pulmonary epithelial cells.The highly conserved heat shock protein HSP-70 can limit all three responses but is not appropriately expressed in the lungs after cecal ligation and double puncture (2CLP), a clinically relevant model of ARDS.We hypothesize that restoring expression of HSP-70 using adenovirus-mediated gene therapy will limit pulmonary pathology following 2CLP.We administered a vector containing the porcine HSP-70 cDNA driven by a CMV promoter (AdHSP) into the lungs of rats subjected to 2CLP or sham operation.Administration of AdHSP after either sham operation or 2CLP increased HSP-70 protein expression in lung tissue, as determined by immunohistochemistry and Western blot hybridization.Administration of AdHSP significantly attenuated interstitial and alveolar edema and protein exudation and dramatically decreased neutrophil accumulation, relative to a control adenovirus.CLP-associated mortality at 48 hours was reduced by half.Modulation of HSP-70 production reduces pathologic changes and may improve outcome in experimental ARDS.
Bus and Breaker Fail Protection for Industrial and Commercial Power Systems Part II: Breaker Fail Protection and Conclusion Working Group Report (2007)

This paper discusses bus and breaker fail protection in modern industrial and commercial power systems. Fault current levels are increasing due to increases in power transmission system capacities and in-plant power generation. Such high fault currents as well as in-plant generator dynamics and system arc flash incident energy limiting requirements necessitate fast bus fault clearing. Meanwhile advancements of numerical relays proved more appealing economically and technically for MV and LV bus protection applications. On the other hand, when bus and breaker fail protection operate, the interrupted segment of the system is larger than a single feeder protection's operation. Consequently, mal-operations of either bus or breaker fail protection are less tolerable than mal-operations of single feeder schemes. This paper is intended to aid protection engineers in making decisions; first if dedicated fast bus and breaker fail protection schemes are required and second what concerns need to be addressed during the selection and the design of such systems. The topics are presented in two separate parts. Part 1 covers scope, introduction and bus protection topics. Part 2 covers breaker fail protection concepts, conclusions and bibliography of additional reference material.
A Disposable Planar Paper‐Based Potentiometric Ion‐Sensing Platform (2016)

Ion-selective electrodes (ISEs) are widely used tools for fast and accurate ion sensing. Herein their design is simplified by embedding a potentiometric cell into paper, complete with an ISE, a reference electrode, and a paper-based microfluidic sample zone that offer the full function of a conventional ISE setup. The disposable planar paper-based ion-sensing platform is suitable for low-cost point-of-care and in-field testing applications. The design is symmetrical and each interfacial potential within the cell is well defined and reproducible, so that the response of the device can be theoretically predicted. For a demonstration of clinical applications, paper-based Cl(-) and K(+) sensors are fabricated with highly reproducible and linear responses towards different concentrations of analyte ions in aqueous and biological samples. The single-use devices can be fabricated by a scalable method, do not need any pretreatment prior to use, and only require a sample volume of 20 μL.
A hypothesized role for dysregulated bradykinin signaling in COVID‐19 respiratory complications (2020)

As of April 20, 2020, over time, the COVID-19 pandemic has resulted in 157 970 deaths out of 2 319 066 confirmed cases, at a Case Fatality Rate of ~6.8%. With the pandemic rapidly spreading, and health delivery systems being overwhelmed, it is imperative that safe and effective pharmacotherapeutic strategies are rapidly explored to improve survival. In this paper, we use established and emerging evidence to propose a testable hypothesis that, a vicious positive feedback loop of des-Arg(9)-bradykinin- and bradykinin-mediated inflammation → injury → inflammation, likely precipitates life threatening respiratory complications in COVID-19. Through our hypothesis, we make the prediction that the FDA-approved molecule, icatibant, might be able to interrupt this feedback loop and, thereby, improve the clinical outcomes. This hypothesis could lead to basic, translational, and clinical studies aimed at reducing COVID-19 morbidity and mortality.
Designing highly multiplex PCR primer sets with Simulated Annealing Design using Dimer Likelihood Estimation (SADDLE) (2022)

One major challenge in the design of highly multiplexed PCR primer sets is the large number of potential primer dimer species that grows quadratically with the number of primers to be designed. Simultaneously, there are exponentially many choices for multiplex primer sequence selection, resulting in systematic evaluation approaches being computationally intractable. Here, we present and experimentally validate Simulated Annealing Design using Dimer Likelihood Estimation (SADDLE), a stochastic algorithm for design of multiplex PCR primer sets that minimize primer dimer formation. In a 96-plex PCR primer set (192 primers), the fraction of primer dimers decreases from 90.7% in a naively designed primer set to 4.9% in our optimized primer set. Even when scaling to 384-plex (768 primers), the optimized primer set maintains low dimer fraction. In addition to NGS, SADDLE-designed primer sets can also be used in qPCR settings to allow highly multiplexed detection of gene fusions in cDNA, with a single-tube assay comprising 60 primers detecting 56 distinct gene fusions recurrently observed in lung cancer.
Targeted Catalytic Inactivation of Angiotensin Converting Enzyme by Lisinopril-Coupled Transition-Metal Chelates (2011)

A series of compounds that target reactive transition-metal chelates to somatic angiotensin converting enzyme (sACE-1) have been synthesized. Half-maximal inhibitory concentrations (IC(50)) and rate constants for both inactivation and cleavage of full-length sACE-1 have been determined and evaluated in terms of metal chelate size, charge, reduction potential, coordination unsaturation, and coreactant selectivity. Ethylenediaminetetraacetic acid (EDTA), nitrilotriacetic acid (NTA), 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA), and tripeptide GGH were linked to the lysine side chain of lisinopril by 1-ethyl-3-[3-(dimethylamino)propyl]carbodiimide hydrochloride/N-hydroxysuccinimide coupling. The resulting amide-linked chelate-lisinopril (EDTA-lisinopril, NTA-lisinopril, DOTA-lisinopril, and GGH-lisinopril) conjugates were used to form coordination complexes with iron, cobalt, nickel, and copper, such that lisinopril could mediate localization of the reactive metal chelates to sACE-1. ACE activity was assayed by monitoring cleavage of the fluorogenic substrate Mca-RPPGFSAFK(Dnp)-OH, a derivative of bradykinin, following preincubation with metal chelate-lisinopril compounds. Concentration-dependent inhibition of sACE-1 by metal chelate-lisinopril complexes revealed IC(50) values ranging from 44 to 4500 nM for Ni-NTA-lisinopril and Ni-DOTA-lisinopril, respectively, versus 1.9 nM for lisinopril. Stronger inhibition was correlated with smaller size and lower negative charge of the attached metal chelates. Time-dependent inactivation of sACE-1 by metal chelate-lisinopril complexes revealed a remarkable range of catalytic activities, with second-order rate constants as high as 150,000 M(-1) min(-1) (Cu-GGH-lisinopril), while catalyst-mediated cleavage of sACE-1 typically occurred at much lower rates, indicating that inactivation arose primarily from side chain modification. Optimal inactivation of sACE-1 was observed when the reduction potential for the metal center was poised near 1000 mV, reflecting the difficulty of protein oxidation. This class of metal chelate-lisinopril complexes possesses a range of high-affinity binding to ACE, introduces the advantage of irreversible catalytic turnover, and marks an important step toward the development of multiple-turnover drugs for selective inactivation of sACE-1.
Balance in Business Reengineering: An Empirical Study of Fit and Performance (1997)

:This study addresses the complex relationship between fit and organizational performance in business reengineering. First, a framework for analysis based on the concept of fit is proposed. Three generic archetypes for three levels of ambitions are defined. Archetypes or ideal-type patterns of change are consistent packages of design and change management measures. It is hypothesized that organizations that change according to an ideal-type pattern outperform organizations that follow a different, inconsistent pattern. On the basis of a questionnaire sent to organizations involved in reengineering, this paper shows that consistent reengineering endeavors generally result in greater benefits than do inconsistent change efforts. It also demonstrates that only a minority of organizations have succeeded in creating a “magical mix” between the level of ambition and the design and change management measures actually taken. Finally, the managerial implications and future research challenges are described.
Approaches to heart valve tissue engineering scaffold design (2007)

Heart valve disease is a significant cause of mortality worldwide. However, to date, a nonthrombogenic, noncalcific prosthetic, which maintains normal valve mechanical properties and hemodynamic flow, and exhibits sufficient fatigue properties has not been designed. Current prosthetic designs have not been optimized and are unsuitable treatment for congenital heart defects. Research is therefore moving towards the development of a tissue engineered heart valve equivalent. Two approaches may be used in the creation of a tissue engineered heart valve, the traditional approach, which involves seeding a scaffold in vitro, in the presence of specific signals prior to implantation, and the guided tissue regeneration approach, which relies on autologous reseeding in vivo. Regardless of the approach taken, the design of a scaffold capable of supporting the growth of cells and extracellular matrix generation and capable of withstanding the unrelenting cardiovascular environment while forming a tight seal during closure, is critical to the success of the tissue engineered construct. This paper focuses on the quest to design, such a scaffold.
Bit Balling Mitigation in PDC Bit Design (2008)

Abstract Historically, parameters like junk slot area (JSA), face volume (FV) and hydraulic horsepower per square inch of bit area (HSI) have been used to empirically predict the balling performance of drill bits. These performance gauges, however, generally produce inconsistent results. This paper presents new design features, guided by numerical predictions and the physical properties of actual PDC bit cuttings that reduce bit balling in PDC bits while drilling shales in water-based drilling muds. Validated by drilling simulator tests and field results, the new design criteria are shown to provide consistent reliable methods to reduce PDC bit balling. The new design methods employ empirical design rules that govern the allowable proximity of two adjacent blades (pinch points) and the profile of the junk slots. These methods are shown to increase the maximum achievable ROP (that ROP where bit balling just begins) by as much as an order of magnitude. Highly controlled and repeatable drilling simulator tests show that failure to employ the new design rules consistently results in premature bit balling. The drilling simulator provides accurate results for bit sizes up to 12 1/4 in. although the principles most likely apply to all bit sizes. Additionally, studies using computational fluid dynamics (CFD) in concert with drilling simulator tests indicate that the maximum achievable balling ROP of a PDC drill bit is increased with increased fluid velocity across the cutting structure. The correlation is shown to be valid over a wide range of bit types and sizes. The new bit designs incorporate strategic nozzle placements and new nozzle designs that improve PDC drill bit performance by increasing the fluid velocity across the cutters.
High-gain direct-coupled matrix distributed amplifier using active feedback topology (2005)

A high-gain matrix distributed amplifier (DA) was developed using a commercial GaAs PHMET foundry for 40-Gb/s baseband applications. Two tiers of matrix DA are directly coupled using a lumped DC level-shift circuit. The DC bias level of the second tier can be tuned using the level-shift circuit for optimum gain. The gain of the matrix DA has been optimized using an active feedback cascode topology, which allows the gain-bandwidth product (GBWP) to be maximized while avoiding instability problems. In order to improve the gain, the second tier is shifted to the output load. The fabricated single-chip matrix DA with a size of 2.4 mm /spl times/ 1.4 mm showed a high gain of 19 dB, and an average noise figure of 5.7 dB with a 48-GHz bandwidth. The GBWP is 428 GHz, which corresponds to the highest performance using GaAs technology for matrix DAs.
Peripferal Nerve Regeneration in Response to Synthesized Nanofiber Scaffold Hydrogel (2012)

1,* nghuidrch@163.com me ; Abstract: Background: earlier studies have demonstrated that Peptide RADA16-I (AcN-RADARADARADARADA-CONH2 ) could repair spinal cord injury and optical pathway and restore visual function. The objective of the research is to investigate the role of RADA16-I in the regeneration of peripheral nerve injury in rats. Method: sciatic nerve injury was performed at female Sprague-Dawley rats. Two groups were conducted: crush group and sever group. There were RADA16-I treatment and blank control in each group. Sense Nerve Conduction (SNCV) parameters were EVALUATED on day 30 in the injured sciatic nerve by means of electrophysiological recording. The results indicates that SNCV in test groups shows a faster healing rate than blank control. Conclusion: self-assembling peptide plays a effective role in the regeneration of sciatic nerve in rats.
Frequency based wind turbine gearbox fault detection applied to a 750 kW wind turbine (2014)

Reliability and availability of modern wind turbines are of increasing importance, for two reasons. The first is due to the fact that power grids around in the world depends at a higher and higher degree on wind energy, and the second is the importance of lowering Cost of Energy of the wind turbines. One of the critical components in modern wind turbines is the gearbox. Failures in the gearbox are costly both due to the cost of the gearbox itself, but also due to lost power generation during repair of it. Wind turbine gearboxes are consequently monitored by condition monitoring systems operating in parallel with the control system, and also uses additional sensors measuring different accelerations and noises, etc. In this paper gearbox data from high fidelity gearbox model of a 750 kW wind turbine gearbox, simulated with and without faults are used to shown the potential of frequency based detection schemes applied on measurements normally available in a wind controller system. This paper shows that two given faults in the gearbox can be detected using a frequency based detection approach applied to sensor signals normally available in the wind turbine control system. This means that gearbox condition monitoring/ fault detection could be included in the standard control system, which potentially can remove the cost of the additional condition monitoring system and the additional sensors used in it.
Identification of unique sensitizing targets for anti‐inflammatory <scp>CDDO</scp>‐<scp>M</scp>e in metastatic melanoma by a large‐scale synthetic lethal <scp>RNA</scp>i screening (2012)

CDDO-Me has been shown to exert potent anti-inflammatory activity for chronic kidney disease and antitumor activity for several tumors, including melanoma, in early clinical trials. To improve CDDO-Me response in melanoma, we utilized a large-scale synthetic lethal RNAi screen targeting 6000 human druggable genes to identify targets that would sensitize melanoma cells to CDDO-Me. Based on screening results, five unique genes (GNPAT, SUMO1, SPINT2, FLI1, and SSX1) significantly potentiated the growth inhibitory effects of CDDO-Me and induced apoptosis in A375, a BRAF mutated melanoma line (P < 0.001). These five genes were then individually validated as targets to potentiate CDDO-Me activity, and related downstream signaling pathways of these genes were analyzed. In addition, the levels of phosphorylated Erk1/2, Akt, GSK-2, and PRAS40 were dramatically decreased by downregulating each of these five genes separately, suggesting a set of common mediators. Our findings indicate that GNPAT, SUMO1, SPINT2, FLI1, and SSX1 play critical roles in synergy with inflammation pathways in modulating melanoma cell survival and could serve as sensitizing targets to enhance CDDO-Me efficacy in melanoma growth control.
Confirmation of glufosinate-resistant Palmer amaranth and response to other herbicides (2022)

Abstract The ability of weed populations to evolve resistance to herbicides affects management strategies and the profitability of crop production. The objective of this research was to screen Palmer amaranth accessions from Arkansas for glufosinate resistance. Additional efforts focused on the effectiveness of various herbicides, across multiple sites of action (SOAs), on each putative-resistant accession. The three putative accessions were selected from 60 Palmer amaranth accessions collected in 2019 and 2020 and screened with to 0.5× and 1× rates of glufosinate. A dose-response experiment was conducted for glufosinate on accessions A2019, A2020, and B2020. The effectiveness of various preemergence- and postemergence-applied herbicides were evaluated on each accession. Resistance ratios of A2019, A2020, and B2020 to glufosinate ranged from 5.1 to 27.4 when comparing LD 50 values to two susceptible accessions, thus all three accessions were resistant to glufosinate. All three accessions (A2019, A2020, and B2020) were found to have a reduction equal to or greater than 20 percentage points in mortality to at least one herbicide from five different SOAs equal to or greater than five sites of action. Herbicides from nine different SOAs controlled A2019 at least 20 percentage points less than the susceptible accessions, which points to a need for additional research to characterize the response of this accession.
The Recurrent Cascade-Correlation architecture (2018)

Abstract: "Recurrent Cascade-Correlation (RCC) is a recurrent version of the Cascade-Correlation learning architecture of Fahlman and Lebiere [Fahlman, 1990]. RCC can learn from examples to map a sequence of inputs into a desired sequence of outputs. New hidden units with recurrent connections are added to the network one at a time, as they are needed during training. In effect, the network builds up a finite-state machine tailored specifically for the current problem. RCC retains the advantages of Cascade-Correlation: fast learning, good generalization, automatic construction of a near-minimal multi-layered network, and the ability to learn complex behaviors through a sequence of simple lessons.The power of RCC is demonstrated on two tasks: learning a finite- state grammar from examples of legal strings, and learning to recognize characters in Morse code."
Developing an upstream process for a monoclonal antibody including medium optimization (2013)

Art. P34, 3 S.
Health-related quality of life burden of women with endometriosis: a literature review (2006)

Endometriosis impairs HRQL, especially in the domains of pain, psychological and social functioning. Therapies have been shown to alleviate symptoms and improve HRQL. Further research is warranted to evaluate the impact of endometriosis on HRQL in adolescents and the impact of infertility due to endometriosis on HRQL.