gebhart/patrepeval huggingface dataset:
Emerging Health Properties of Whey Proteins and Their Clinical Implications (2007)

The nursery rhyme "Little Miss Muffet sat on a tuffet (small stool) eating her curds and whey. ..." is recognition of the fact that over the centuries "curds and whey", the two major components of cow's milk, have been widely accepted as part of a healthy diet. Milk provides complete nourishment for the neonate for six months from birth, containing factors that help develop various organ systems including the brain, immune system, and the intestine. Importantly it provides immune protection at a time when the neonates own immune system, though fully developed, is albeit immature. Many adult consumers include cow's milk as part of a healthy diet as it provides protein and essential nutrients, vitamins, and minerals, in particular calcium for strong bones. There is a growing appreciation that milk, and in particular whey, contains components that not only provide nutrition, but can also prevent and attenuate disease, or augment conventional therapies, when delivered in amounts that exceed normal dietary intakes. This paper reviews the emerging health properties of whey proteins and their clinical implications.
Formalin-Fixed and Heat-Retrieved Tissue Antigens: A Comparison of Their Immunoreactivity in Experimental Antibody Diluents (2001)

Heat-induced antigen retrieval procedures have become an effective and therefore important part of immunohistochemistry. Although used with increasing frequency, it is not known by what mechanism or to what extent these procedures restore the molecular integrity of the antigen. In an attempt to explore these questions, the immune reactions of 14 tissue antigens with their corresponding monoclonal antibodies were compared both in the absence and after the application of two widely used heat-induced antigen retrieval procedures. The tissue antigens were selected either because of the increased or lack of response to heat retrieval. The nature of their immune reactions was appraised through the variable effects that some diluent solutes were previously shown to have on monoclonal antibodies at different pH. It was found that on heat retrieval, four antigens reacting with monoclonal antibodies of subclass IgG2a or IgG1 reversed their preference for an alkaline pH to that of an acid pH. Eight of nine antigens reacting with monoclonal antibodies of subclass IgG1 and one antigen reacting with an antibody of class IgM remained most reactive at pH 6.0. The retrieved epitope of a B-cell antigen reacting with an IgG3 subclass antibody retained its preference for the alkaline pH. These patterns of response by the antigens to heat-induced retrieval are compared with the previously reported modifications in the immune reactions of monoclonal antibodies when sodium chloride and sodium phosphates were included in the antibody diluent buffer. Use of antibody diluents containing these solutes in combination with heat-induced antigen retrieval resulted in the loss of most of the sensitivity gained through this technique. The possible common mechanisms underlying the causes for these results are discussed.
Графічна оболонка для побудови введених користувачем арифметичних функцій (2023)

Стаття має актуальну тематику в науково-практичному аспекті розробки графічної оболонки програмного застосунку для побудови функцій двох змінних, введених користувачем. Обґрунтовано вибір мови програмування та використання програмного інтерфейсу OpenGL. Досліджено якість побудови каркасу функції в залежності від кроку розрахунків. Запропоновано методику перерахунку координат функції для застосованого програмного інтерфейсу. Проаналізовано масштабування піддослідної функції та використання шейдерів геометрії мови GLSL для створення імітації освітлення.Запропоновано технологію визначення освітлення через обчислення нормалей до площин графічних примітивів, що стало можливим через специфічну послідовність введення вершин у буфери. Обчислено векторні добутки для катетів графічних примітивів, що утворюють зображення, та знайдено проекції для визначення освітлення. Досліджено результати візуалізації для певного набору функцій. Запропоновано шляхи відображення пояснювального тексту у програмі. Запропоновано методику зчитування файлу чотириканального зображення для завантаження зображення упрограмний застосунок. Метою роботи є створення графічної оболонки програмного застосунку для побудови функцій двох змінних, введених користувачем. Бібл. 5, іл. 7, табл. 2
Manufacturing Dendritic Cells for Immunotherapy: Monocyte Enrichment (2020)

Dendritic cells play a key role in activation of the immune system as potent antigen-presenting cells. This pivotal position, along with the ability to generate dendritic cells from monocytes and ready uptake of antigen, makes them an intriguing vehicle for immunotherapy for a variety of indications. Since the first reported trial using dendritic cells in 1995, they have been used in trials all over the world for a plethora of indications. Monocyte-derived dendritic cells are generated from whole blood or apheresis products by culturing enriched monocytes in the presence of interleukin (IL)-4 and granulocyte-macrophage colony-stimulating factor (GM-CSF). A variety of methods can be used for enrichment of monocytes for generation of clinical-grade dendritic cells and are summarized herein.
Identification of phosphorylated sites in the mouse glucocorticoid receptor. (1991)

Glucocorticoid receptors in vivo are phosphorylated in the absence of hormone and become hyperphosphorylated in the presence of glucocorticoid agonist but not antagonists (Ortí, E., Mendel, D.B., Smith, L.I., and Munck, A. (1989) J. Biol. Chem. 264, 9728-9731). As a preliminary step to elucidating the functional significance of receptor phosphorylation, we have identified seven phosphorylated sites on the mouse receptor. Tryptic phosphopeptides from 32P-labeled receptors were purified from glucocorticoid-treated mouse thymoma cells (WEHI-7) and from stably transfected Chinese hamster ovary cells (WCL2) that express large numbers of mouse receptors. Phosphopeptide maps of receptors from these two cell types were almost indistinguishable. Solid phase sequencing revealed phosphorylation at serines 122, 150, 212, 220, 234, and 315 and threonine 159. Serines 122, 150, 212, 220, and 234 and the sequences surrounding them are conserved in the homologous regions of the rat and human receptors, but threonine 159 and serine 315 have no homologues in the human receptor. The seven phosphorylated sites are in the amino-terminal domain of the receptor. All but serine 315 are within transactivation domains identified in the human and/or rat receptors. Serines 212, 220, and 234 are in a highly acidic region that in the mouse receptor is necessary for full transcription initiation activity and reduces nonspecific DNA binding. Serines 212, 220, and 234 and threonine 159 are in consensus sequences for proline-directed kinase and/or p34cdc2 kinase. Serine 122 is in a consensus sequence for casein kinase II whereas serines 150 and 315 do not appear to be in any known kinase consensus sequence. The location of many of these sites suggests a role of phosphorylation in transactivation.
Optimum Design of Compound Helicopters that Use Higher Harmonic Control (2015)

The optimal design of a compound helicopter comprised of counterrotating coaxial rotors, a propeller, and optionally a fixed wing is investigated. The blade geometry, azimuthal blade pitch inputs, optimal shaft angle (rotor angle of attack), and division of propulsive and lifting forces among the components that minimize the total power for a given flight condition are determined. The optimal design problem is cast as a variational statement that minimizes the sum of induced and viscous power losses for a prescribed lift, propulsive force, and vehicle trim condition. The rotor, propeller, and wing geometry and control inputs are related to the far-field circulation through a lifting-line model that accounts for experimentally or computationally determined nonlinear lift and drag polars. The variational statement is discretized using a vortex lattice wake, and the resulting nonlinear constrained optimization problem is solved via Newton iteration. Results show that varying the prescribed propulsive force of the system affects the optimal shaft angle and rotor design, that higher harmonic control reduces the total vehicle power loss (inefficiency) in high-speed flight by as much as 15%, and that imposing a maximum allowable lateral lift offset can greatly increase the power loss of the coaxial rotors.
Automatic tracking of implanted fiducial markers in cone beam CT projection images (2012)

The proposed method has accuracy comparable to that of manual tracking and, in combination with the proposed batch postprocessing, superior robustness. Marker tracking in cone beam CT (CBCT) projections is useful for a variety of purposes, such as providing data for assessment of intrafraction motion, target tracking during rotational treatment delivery, motion correction of CBCT, and phase sorting for 4D CBCT.
CrowdOLR: Toward Object Location Recognition With Crowdsourced Fingerprints Using Smartphones (2017)

Recognizing object location by taking a photo with smartphones is useful for many location-based services. However, start-of-the-art technologies for both localization and location recognition have difficulty in achieving satisfactory performance. Moreover, it is a challenging issue to construct and maintain a large-scale image database for existing visual-based location recognition systems. To cope with these issues, we introduce CrowdOLR, a crowdsourcing based object location recognition system, which collects one location image together with various rich sensory data (GPS coordinates, azimuth angle, tilt angle, etc.) as a fingerprint of a location query and matches it to a fingerprint database crowdsourced from users' smartphones. We designed a simple and efficient user action mode and proposed a series of fingerprint extracting, searching, and matching methods, so that CrowdOLR satisfies five desirable properties: high recognition accuracy, user friendliness, quick response, no/little site survey, and timely update. We implemented CrowdOLR and collected 8100 location fingerprints of 162 objects for performance evaluation. Extensive experiments demonstrate that CrowdOLR achieves promising results in various complicated and realistic scenarios.
A Study of 4G Radiofrequency Radiation effects on Juvenile Wistar Rats Cerebellum and Potential Attenuative Properties of Fish Oil Omega-3 Fat (2021)

Introduction: Technological devices have become a very important part of our everyday life. These electronic devices create an artificial electromagnetic field (EMF) and emit radiofrequency radiations (RFR) which have been reported to have deleterious effects on various tissues in living organisms. Fish oil is a rich source of the n-3 (also known as omega-3) fatty acids, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). The cerebellum plays an important role in motor control and the coordination, precision and timing of movements, as well as in motor learning. This study investigated the effect of radiofrequency radiation on the structures of the cerebellum of adolescent Wistar rats, its effect on pre-pubertal development and the potential attenuative properties of fish oil omega-3 fat. Materials and Methods: Forty (n=40) adolescent Wistar rats of approximately 35 days old were divided into five groups labelled A-E. A 4G RFR-emitting WI-FI device served as the RFR source. The 4G radiofrequency transmitter was installed at a distance of 0.5 meters from the base of the cage housing the experimental animals in each group. The fish oil was administered orally to the animals. Rats were sacrificed after 28 days of treatment and their brains harvested. The cerebellar tissues were excised and processed for histological, immunohistochemical and biochemical observations. Neurobehavioural studies including Barnes Maze and beam walk were carried out before sacrifice. Results: 4G radiofrequency radiation caused distortions in the morphology and spatial arrangement of Purkinje cells in the cerebellar tissues. It also induced neuroinflammation as marked by enhanced astrocyte reactions. Also, behavioural aberrations including negative changes in key memory parameters such as increased latency and error poke in Barnes maze assays, and increased movement parameter errors were attributable to FRR effects. Also, aberrations in neurotransmitters activities levels and vital neurochemistry enzymes were observed. The administered Omega-3 fat had an attenuative effect on the affected cortical structures, neurochemistry and selected neurobehavioural parameters.
B7-H1 (Programmed Cell Death Ligand 1) Is Required for the Development of Multifunctional Th1 Cells and Immunity to Primary, but Not Secondary, <i>Salmonella</i> Infection (2010)

Robust Ab and CD4 T cell responses are required for the resolution of Salmonella infection in susceptible mice. In this study, we examined the role of B7-H1 (programmed cell death ligand 1) in resistance to primary Salmonella infection. Infected B7-H1-deficient mice had significantly higher bacterial burdens at day 21 and day 35 postinfection compared with wild-type mice, demonstrating that B7-H1 plays an important role in immunity to Salmonella. B7-H1-deficient and wild-type mice both generated Salmonella-specific IgM and IgG2c Ab responses to infection, and clonal expansion of endogenous and adoptively transferred Salmonella-specific CD4 T cells was similar in both groups. However, although Salmonella-specific IFN-gamma-producing Th1 CD4 T cells were generated in Salmonella-infected B7-H1-deficient mice, these cells did not expand to the level observed in wild-type mice. Furthermore, fewer multifunctional Th1 cells that simultaneously secreted IFN-gamma, TNF-alpha, and IL-2 were detected in Salmonella-infected B7-H1-deficient mice. Together, these data demonstrate that B7-H1 is required for the generation of multifunctional Th1 responses and optimal protective immunity to primary Salmonella infection.
Robotic Arm-assisted UKA Improves Tibial Component Alignment: A Pilot Study (2009)

Level III, therapeutic study. See Guidelines for Authors for a complete description of levels of evidence.
Efficient use of DNA molecular markers to construct industrial yeast strains (2007)

Saccharomyces cerevisiae yeast strains exhibit a huge genotypic and phenotypic diversity. Breeding strategies taking advantage of these characteristics would contribute greatly to improving industrial yeasts. Here we mapped and introgressed chromosomal regions controlling industrial yeast properties, such as hydrogen sulphide production, phenolic off-flavor and a kinetic trait (lag phase duration). Two parent strains derived from industrial isolates used in winemaking and which exhibited significant quantitative differences in these traits were crossed and their progeny (50-170 clones) was analyzed for the segregation of these traits. Forty-eight segregants were genotyped at 2212 marker positions using DNA microarrays and one significant locus was mapped for each trait. To exploit these loci, an introgression approach was supervised by molecular markers monitoring using PCR/RFLP. Five successive backcrosses between an elite strain and appropriate segregants were sufficient to improve three trait values. Microarray-based genotyping confirmed that over 95% of the elite strain genome was recovered by this methodology. Moreover, karyotype patterns, mtDNA and tetrad analysis showed some genomic rearrangements during the introgression procedure.
Folic acid-conjugated mesoporous silica nanoparticles for enhanced therapeutic efficacy of topotecan in retina cancers (2018)

Wei Qu,1 Bo Meng,1 Yangyang Yu,2 Shaowei Wang1 1Department of Ophthalmology, The 2nd Affiliated Hospital of Harbin Medical University, 2Department of Ophthalmology, Heilongjiang Provincial Hospital, Harbin, Heilongjiang, People’s Republic of China Abstract: In this study, topotecan-loaded mesoporous silica nanoparticles were prepared and surface conjugated with folic acid (FTMN) to enhance the therapeutic efficacy of topotecan for the treatment of retinoblastoma (RB) cancers. The particles were nano-sized and exhibited a sustained release of drug in the physiological conditions. The folic acid-conjugated nanoformulations exhibited a remarkable uptake in RB cells compared to that of non-targeted nanoparticles. These results clearly indicate that receptor-mediated endocytosis is the mechanism of cellular internalization. The greater cellular uptake of FTMN resulted in significantly higher cytotoxic effect in Y79 cancer cells compared to that of other formulations. The results were well corroborated with the live/dead assay and nuclear fragmentation assay. FTMN consistently induced apoptosis of cancer cells with an efficiency of ~58%. Our results clearly showed that nanoparticulate encapsulation of TPT exhibited superior anticancer efficacy in Y79 cancer cells compared to that of free drug or non-targeted nanoparticles. As expected, FTMN exhibited a remarkable reduction in the overall tumor volume compared to any other group with less presence of tumor cells in histology staining. Overall, folic acid-conjugated nanoparticulate system could provide an effective platform for RB treatment. Keywords: retinoblastoma, topotecan, mesoporous silica nanoparticles, apoptosis, anticancer effect
Effects of<i>Lactobacillus acidophilus</i>NCFM on insulin sensitivity and the systemic inflammatory response in human subjects (2010)

According to animal studies, intake of probiotic bacteria may improve glucose homeostasis. We hypothesised that probiotic bacteria improve insulin sensitivity by attenuating systemic inflammation. Therefore, the effects of oral supplementation with the probiotic bacterium Lactobacillus acidophilus NCFM on insulin sensitivity and the inflammatory response were investigated in subjects with normal or impaired insulin sensitivity. In a double-blinded, randomised fashion, forty-five males with type 2 diabetes, impaired or normal glucose tolerance were enrolled and allocated to a 4-week treatment course with either L. acidophilus NCFM or placebo. L. acidophilus was detected in stool samples by denaturating gradient gel electrophoresis and real-time PCR. Separated by the 4-week intervention period, two hyperinsulinaemic-euglycaemic clamps were performed to estimate insulin sensitivity. Furthermore, the systemic inflammatory response was evaluated by subjecting the participants to Escherichia coli lipopolysaccharide injection (0·3 ng/kg) before and after the treatment course. L. acidophilus NCFM was detected in 75 % of the faecal samples after treatment with the probiotic bacterium. Insulin sensitivity was preserved among volunteers in the L. acidophilus NCFM group, whereas it decreased in the placebo group. Both baseline inflammatory markers and the systemic inflammatory response were, however, unaffected by the intervention. In conclusion, intake of L. acidophilus NCFM for 4 weeks preserved insulin sensitivity compared with placebo, but did not affect the systemic inflammatory response.
The structure of a separating turbulent boundary layer. Part 1. Mean flow and Reynolds stresses (1981)

The problem of turbulent-boundary-layer separation due to an adverse pressure gradient is an old but still important problem in many fluid flow devices. Until recent years little quantitative experimental information was available on the flow structure downstream of separation because of the lack of proper instrumentation. The directionally sensitive laser anemometer provides the ability to measure the instantaneous flow direction and magnitude accurately. The experimental results described here are concerned with a nominally two-dimensional, separating turbulent boundary layer for an airfoil-type flow in which the flow was accelerated and then decelerated until separation. Upstream of separation single and cross-wire hot-wire anemometer measurements are also presented. Measurements in the separated zone with a directionally sensitive laser-anemometer system were obtained for U, V , $\overline{u^2}, \overline{v^2}, - \overline{uv}$ , the fraction of time that the flow moves downstream, and the fraction of time that the flow moves away from the wall. In addition to confirming the earlier conclusions of Simpson, Strickland & Barr (1977) regarding a separating airfoil-type turbulent boundary layer, much new information about the separated region has been gathered. (1) The backflow mean velocity profile scales on the maximum negative mean velocity U N and its distance from the wall N . A U + vs. y + law-of-the-wall velocity profile is not consistent with this result. (2) The turbulent velocities are comparable with the mean velocity in the backflow, although low turbulent shearing stresses are present. (3) Mixing length and eddy viscosity models are physically meaningless in the backflow and have reduced values in the outer region of the separated flow. Downstream of fully developed separation, the mean backflow appears to be divided into three layers: a viscous layer nearest the wall that is dominated by the turbulent flow unsteadiness but with little Reynolds shearing stress effects; a rather flat intermediate layer that seems to act as an overlap region between the viscous wall and outer regions; and the outer backflow region that is really part of the large-scaled outer region flow. The Reynolds shearing stress must be modelled by relating it to the turbulence structure and not to local mean velocity gradients. The mean velocities in the backflow are the results of time averaging the large turbulent fluctuations and are not related to the source of the turbulence.
Application of Direct-Fed Microbial Bacteria and Fructooligosaccharides for Salmonella Control in Broilers During Feed Withdrawal (1996)

Providing direct-fed-microbial (DFM) bacteria and fructooligosaccharides (FOS) for the control of potential escalation of Salmonella colonization during simulated feed withdrawal and confinement was assessed. Eight hundred and eighty broilers (16 pens; 55 chicks per pen) were reared to 6 wk of age. Chicks were sprayed with a solution containing 10(6) nalidixic-acid resistant Salmonella typhimuriumNR cells per milliliter on the 2nd d after hatching. Because this first challenge did not yield a high infection rate, chickens were rechallenged per Os at Day 18 by providing water containing 10(7) cells of S. typhimuriumNR per milliliter. At 3 and 5 wk of age, 10 birds per pen were euthanatized and cecal Salmonella were quantified (log colony-forming units per gram). Feed was removed from all pens at 6 wk, and pens were randomly assigned to be either the treatment group or the control group. The treatment groups were provided a DFM (mixture of nine bacteria) and FOS 50 (R) (10%) in the drinking water. The control groups received drinking water only. After 6 h of feed withdrawal, chickens were cooped (eight per coop) and held 10 h. Immediately after confinement, 10 chickens were used for cecal enumeration of S. typhimuriumNR. Salmonella colonization declined from 99% at 3 wk to 44% at 5 wk. After feed withdrawal, application of the treatment, and confinement, 11 and 14% of the treated and control groups, respectively, yielded S. typhimuriumNR by direct plating from ceca (3.87 and 3.75 log 10 cfu/g, respectively). No difference (P > 0.05) in Salmonella colonization occurred between the treated and the control groups; however, enrichment of ceca (incubation in nutrient broth at 37 C for 24 h) yielded a higher incidence of S. typhimuriumNR in the control groups (32% in the treated vs 51% in the control). Ceca weights were greater in the treated group (P < 0.05). Simulated feed withdrawal and confinement did not escalate Salmonella colonization in the chicken ceca.