gebhart/patrepeval huggingface dataset:
Taste Receptor Cell Responses to the Bitter Stimulus Denatonium Involve Ca<sup>2+</sup> Influx Via Store-Operated Channels (2002)

Previous studies in rat and mouse have shown that brief exposure to the bitter stimulus denatonium induces an increase in [Ca2+]i due to Ca2+ release from intracellular Ca2+ stores, rather than Ca2+ influx. We report here that prolonged exposure to denatonium induces sustained increases in [Ca2+]i that are dependent on Ca2+ influx. Similar results were obtained from taste cells of the mudpuppy, Necturus maculosus, as well as green fluorescent protein (GFP) tagged gustducin-expressing taste cells of transgenic mice. In a subset of mudpuppy taste cells, prolonged exposure to denatonium induced oscillatory Ca2+ responses. Depletion of Ca2+ stores by thapsigargin also induced Ca2+ influx, suggesting that Ca2+ store-operated channels (SOCs) are present in both mudpuppy taste cells and gustducin-expressing taste cells of mouse. Further, treatment with thapsigargin prevented subsequent responses to denatonium, suggesting that the SOCs were the source of the Ca2+ influx. These data suggest that SOCs may contribute to bitter taste transduction and to regulation of Ca2+ homeostasis in taste cells.
Synthesis of Angiotensin II Receptor Blockers by Means of a Catalytic System for C–H Activation (2011)

A highly efficient catalytic system for C-H activation has been worked out that involves inexpensive RuCl(3)·xH(2)O and a specific amount of PPh(3). This procedure has been successfully applied to a practical synthesis of angiotensin II receptor blockers (ARBs). The residual ruthenium that existed in the reaction mixture was thoroughly removed by treatment with properly selected metal scavengers. The new process permits ready access to the important class of drugs in a highly atom-economical and sustainable manner.
Time-domain reflectometry using arbitrary incident waveforms (2002)

A novel time-domain reflectometry technique is developed for detecting the physical structures of transmission lines by using arbitrary waveforms. By discretizing both incident and reflected waves, we formulate the reflection coefficient of a nonuniform transmission line as a polynomial ratio in the Z-transform, wherein the numerator and denominator represent the reflected and incident waves, respectively. A reconstruction scheme is derived to obtain the characteristic impedance profile of a transmission line. Some examples are presented to illustrate the validity of this new technique.
Considerations on contactless conductivity detection in capillary electrophoresis (2002)

Nearly all analyses by capillary electrophoresis (CE) are performed using optical detection, utilizing either absorbance or (laser-induced) fluorescence. Though adequate for many analytical problems, in a large number of cases, e.g., involving non-UV-absorbing compounds, these optical detection methods fall short. Indirect optical detection can then still provide an acceptable means of detection, however, with a strongly reduced sensitivity. During the past few years, contactless conductivity detection (CCD) has been presented as a valuable extension to optical detection techniques. It has been demonstrated that with CCD detection limits comparable, or even superior, to (indirect) optical detection can be obtained. Additionally, construction of the CCD around the CE capillary is straightforward and robust operation is easily obtained. Unfortunately, in the literature a large variety of designs and operating conditions for CCD were described. In this contribution, several important parameters of CCD are identified and their influence on, e.g., detectability and peak shape is described. An optimized setup based on a well-defined detection cell with three detection electrodes is presented. Additionally, simple and commercially available read-out electronics are described. The performance of the CCD-CE system was demonstrated for the analysis of peptides. Detection limits at the microM level were obtained in combination with good peak shapes and an overall good performance and stability.
Development of a Genetic System for <i>Geobacter sulfurreducens</i> (2001)

Members of the genus Geobacter are the dominant metal-reducing microorganisms in a variety of anaerobic subsurface environments and have been shown to be involved in the bioremediation of both organic and metal contaminants. To facilitate the study of the physiology of these organisms, a genetic system was developed for Geobacter sulfurreducens. The antibiotic sensitivity of this organism was characterized, and optimal conditions for plating it at high efficiency were established. A protocol for the introduction of foreign DNA into G. sulfurreducens by electroporation was also developed. Two classes of broad-host-range vectors, IncQ and pBBR1, were found to be capable of replication in G. sulfurreducens. In particular, the IncQ plasmid pCD342 was found to be a suitable expression vector for this organism. When the information and novel methods described above were utilized, the nifD gene of G. sulfurreducens was disrupted by the single-step gene replacement method. Insertional mutagenesis of this key gene in the nitrogen fixation pathway impaired the ability of G. sulfurreducens to grow in medium lacking a source of fixed nitrogen. Expression of the nifD gene in trans complemented this phenotype. This paper constitutes the first report of genetic manipulation of a member of the Geobacter genus.
The law of one price and arbitrage on China's dual-listings (2012)

Traditionally, arbitrage refers to simultaneously buying and selling the same financial assets by taking advantage of a price difference in two or more markets. However, the strict sense of arbitrage is hardly obtained after consideration the issues concerning transaction costs and time value of money. By using the identical assets such as Chinese ADRs and their underlying securities traded in different markets in Hong Kong in HK dollar and in New York in US dollar and by constructing a very simple arbitrage trading strategy, this study demonstrates that arbitrage profits are still available with monthly return ranging from 0.5 per cent to 3.8 per cent after considering transaction costs and non-overlap trading time issues. This is a new study to verify this behaviour of an emerging market's ADRs traded in two financial market locations, so adding evidence of inefficiency in trading of China-listed stocks in foreign locations.
Urinary metabolic signatures of human adiposity (2015)

Obesity is a major public health problem worldwide. We used 24-hour urinary metabolic profiling by proton ((1)H) nuclear magnetic resonance (NMR) spectroscopy and ion exchange chromatography to characterize the metabolic signatures of adiposity in the U.S. (n = 1880) and UK (n = 444) cohorts of the INTERMAP (International Study of Macro- and Micronutrients and Blood Pressure) epidemiologic study. Metabolic profiling of urine samples collected over two 24-hour time periods 3 weeks apart showed reproducible patterns of metabolite excretion associated with adiposity. Exploratory analysis of the urinary metabolome using (1)H NMR spectroscopy of the U.S. samples identified 29 molecular species, clustered in interconnecting metabolic pathways, that were significantly associated (P = 1.5 × 10(-5) to 2.0 × 10(-36)) with body mass index (BMI); 25 of these species were also found in the UK validation cohort. We found multiple associations between urinary metabolites and BMI including urinary glycoproteins and N-acetyl neuraminate (related to renal function), trimethylamine, dimethylamine, 4-cresyl sulfate, phenylacetylglutamine and 2-hydroxyisobutyrate (gut microbial co-metabolites), succinate and citrate (tricarboxylic acid cycle intermediates), ketoleucine and the ketoleucine/leucine ratio (linked to skeletal muscle mitochondria and branched-chain amino acid metabolism), ethanolamine (skeletal muscle turnover), and 3-methylhistidine (skeletal muscle turnover and meat intake). We mapped the multiple BMI-metabolite relationships as part of an integrated systems network that describes the connectivities between the complex pathway and compartmental signatures of human adiposity.
Cold roll bonding bond strengths: Review (2011)

Cold roll bonding (CRB), a well established and widely used manufacturing process, is a solid state bonding process to join similar and dissimilar metals. The present work offers a review of the CRB process and effective parameters on bond strength of cold roll bonded materials. The effects of different amounts of reduction in thickness, annealing treatment, initial thickness, rolling speed, rolling direction, friction coefficient and presence of particles between strips on bond strength were evaluated. It was found that higher reduction in thickness and friction coefficient, lower initial thickness, rolling speed and amount of particles were the important factors involved in improving bond strength. In addition, annealing treatment before and/or after the CRB process increased bond strength, while the effect of prerolling annealing was more pronounced. Finally, it has been indicated that bond strength of cold roll bonded fcc materials is stronger than that of the bcc and hcp materials.
Reflections on FMS Treatment, Research, and Neurotherapy: Cautionary Tales (2006)

Treatment planning for a patient diagnosed with fibromyalgia (FMS) requires neurotherapists to consider a wide range of potential causes during history taking. Effective treatment planning often involves interventions from multiple specialists coordinating treatments. Creation of a treatment team may involve, in addition to neurotherapy, medical specialties such as infectious disease, physical medicine, neurology, nutrition, and rheumatology, as well as cranial sacral and myofascial treatments, and surface electromyography (sEMG). Understanding the signs of common complications in those diagnosed with FMS is vital to effective treatment.
Improved Synthesis of Buprenorphine from Thebaine and/or Oripavine <i>via</i> Palladium‐Catalyzed N‐Demethylation/Acylation and/or Concomitant O‐Demethylation (2012)

Abstract An improved preparation of buprenorphine via palladium‐catalyzed N‐demethylation/acylation is reported. Three routes were investigated and compared in overall yield. The first involved N‐demethylation/acylation of an advanced intermediate obtained from thebaine followed by hydrolysis of the N ‐acetamide and alkylation with cyclopropylmethyl bromide and/or reduction of the N ‐acetyl group with the Schwartz reagent followed by N‐alkylation. The second route employed cyclopropylcarboxylic acid anhydride in the N‐demethylation/acylation protocol and subsequent reduction of the cyclopropylcarboxamide by either lithium aluminum hydride or under hydrosilylation conditions. Both of these routes originated in thebaine and therefore required O‐demethylation as a final step. The third route employed an N‐demethylation/acylation sequence starting from oripavine rather than thebaine, thus avoiding the O‐demethylation. The routes are compared for overall efficiency and experimental and spectral data are provided for all new compounds.
Grammatical error correction using hybrid systems and type filtering (2014)

This paper describes our submission to the CoNLL 2014 shared task on grammatical error correction using a hybrid approach, which includes both a rule-based and an SMT system augmented by a large webbased language model. Furthermore, we demonstrate that correction type estimation can be used to remove unnecessary corrections, improving precision without harming recall. Our best hybrid system achieves state-of-the-art results, ranking first on the original test set and second on the test set with alternative annotations.
Mast cell regulation of Na-glutamine co-transporters B0AT1 in villus and SN2 in crypt cells during chronic intestinal inflammation (2015)

In the present study we demonstrate that mast cells likely function as a common upstream immune pathway regulator of the Na-dependent glutamine co-transporters, B0AT1 in villus cells and SN2 in crypts cells that are uniquely altered in the chronically inflamed small intestine.
Effective energy function for proteins in solution (1999)

A Gaussian solvent-exclusion model for the solvation free energy is developed. It is based on theoretical considerations and parametrized with experimental data. When combined with the CHARMM 19 polar hydrogen energy function, it provides an effective energy function (EEF1) for proteins in solution. The solvation model assumes that the solvation free energy of a protein molecule is a sum of group contributions, which are determined from values for small model compounds. For charged groups, the self-energy contribution is accounted for primarily by the exclusion model. Ionic side-chains are neutralized, and a distance-dependent dielectric constant is used to approximate the charge-charge interactions in solution. The resulting EEF1 is subjected to a number of tests. Molecular dynamics simulations at room temperature of several proteins in their native conformation are performed, and stable trajectories are obtained. The deviations from the experimental structures are similar to those observed in explicit water simulations. The calculated enthalpy of unfolding of a polyalanine helix is found to be in good agreement with experimental data. Results reported elsewhere show that EEF1 clearly distinguishes correctly from incorrectly folded proteins, both in static energy evaluations and in molecular dynamics simulations and that unfolding pathways obtained by high-temperature molecular dynamics simulations agree with those obtained by explicit water simulations. Thus, this energy function appears to provide a realistic first approximation to the effective energy hypersurface of proteins.
Myocardial infarction: contemporary management strategies (2007)

Myocardial infarction (MI) is a common clinical diagnosis, associated with significant morbidity and mortality, not only in the short term, but also years following the index event. A more complete understanding of the pathophysiology of MI has ushered the era of multipronged treatment approach, with a combination of goal-directed revascularization, a broad adjunctive pharmacological therapy and aggressive secondary prevention measures. The goals of this article are to review the basic pathophysiological processes, which lead up to a clinical diagnosis of MI, to highlight the essential elements of clinical presentation and to summarize the evidence for comprehensive therapy. Emphasis has been placed on the choice of primary reperfusion therapy for ST-elevation MI, on risk-stratification of patients with non-ST elevation MI, and on rationale behind the selection of anti-ischaemic and antithrombotic therapy. Finally, evidence-based approach to secondary prevention is outlined.
Sparse channel estimation via matching pursuit with application to equalization (2002)

Channels with a sparse impulse response arise in a number of communication applications. Exploiting the sparsity of the channel, we show how an estimate of the channel may be obtained using a matching pursuit (MP) algorithm. This estimate is compared to thresholded variants of the least squares (LS) channel estimate. Among these sparse channel estimates, the MP estimate is computationally much simpler to implement and a shorter training sequence is required to form an accurate channel estimate leading to greater information throughput.
Skeleton Decoration of NHCs by Amino Groups and its Sequential Booster Effect on the Palladium‐Catalyzed Buchwald–Hartwig Amination (2014)

A challenging synthetic modification of PEPPSI-type palladium pre-catalysts consisting of a stepwise incorporation of one and two amino groups onto the NHC skeleton was seen to exert a sequential enhancement of the electronic donor properties. This appears to be positively correlated with the catalytic performances of the corresponding complexes in the Buchwald-Hartwig amination. This is illustrated, for example, by the quantitative amination of 4-chloroanisole by morpholine within 2 h at 25 °C with a 2 mol% catalyst/substrate ratio or by a significant reduction of catalytic loading (down to 0.005 mol%) for the coupling of aryl chlorides with anilines (max TON: 19,600).
Optimization of Prebiotics and Oxygen Scavengers for <i>Bifidobacterium bifidum</i> BB01 Microcapsules by Response Surface Methodology (2018)

Abstract The effects of different prebiotics and oxygen scavengers on making the process of xanthan gum and chitosan (XC) Bifidobacterium bifidum BB01 microcapsules were studied by single factor experiment and Plackett-Burman screening test, ascorbic acid, sodium erythorbate and xylo-oligosaccharide had significant effects. Based on the previous studies, the process XC B. Bifidum BB01 microcapsules were further optimized by Box-Behnken model in this study. Response surface analysis showed that the best additive amoumt of ascorbic acid, sodium erythorbate and xylo-oligosaccharide were 3.0%, 2.36% and 4.99%, respectively. The viable counts of B. Bifidum BB01 microcapsules reached to 1.52×10 10 CFU/g from 1.25×10 10 CFU/g, the encapsulation yield reached to 94.88% from 90% under the optimum conditions. It provided the research foundation for the afterward production and exploration of the process XC B. Bifidum BB01 microcapsules.
Mechanical properties of phosphate glasses as a function of the total bonding energy per unit volume of glass (2008)

The dependences of the structural strength and the elastic modulus on the chemical composition of phosphate glasses are analyzed. The structural strength and the elastic modulus of phosphate glasses are determined for different types of both the modifier ion and the second glass-former (B2O3 or SiO2) introduced into the glass. The total bonding energy per unit volume of the glass is calculated from the data on the dissociation energy for the oxides entering into the glass composition and the data on the atomic packing density. For four systems of phosphate glasses, it is demonstrated that the Young’s modulus depends linearly on the total bonding energy per unit volume of the glass. The dependence of the structural strength on the chemical composition exhibits a more complex behavior and is governed by other additional factors, such as the formation of new structural groups or the change in the structure of the anionic network of the glass.
CHAPTER 6: Products from Hard Wheat Flour: Problems, Causes, and Resolutions (2001)

Cereals & Grains Association is a nonprofit organization dedicated to advancing the knowledge and understanding of cereal grain science through research leadership, education, superior technical service, and advocacy.
Microwave-assisted purification of HIPCO carbon nanotubes (2002)

A very easy way for improving the purity of carbon nanotubes using a domestic multimode microwave oven is reported, in which selective burning of metal particles helps remove most of the iron content.
Ultra-sensitive protein detection via Single Molecule Arrays towards early stage cancer monitoring (2015)

The early diagnosis of cancers and continued monitoring of tumor growth would be greatly facilitated by the development of a blood-based, non-invasive, screening technique for early cancer detection. Current technologies for cancer screening and detection typically rely on imaging techniques or blood tests that are not accurate or sensitive enough to definitively diagnose cancer at its earliest stages or predict biologic outcomes. By utilizing Single Molecule Arrays (SiMoA), an ultra-sensitive enzyme-linked immunosorbent assay (ELISA) technique, we were able to measure increasing levels of prostate specific antigen (PSA) within murine serum over time, which we attribute to tumor development. The measured concentrations of PSA were well below the detectable limits of both a leading clinical diagnostic PSA ELISA assay as well as a commercial ultra-sensitive PSA assay. Our work benchmarks the role of SiMoA as a vital tool in monitoring previously non-detectable protein biomarkers in serum for early cancer detection and offers significant potential as a non-invasive platform for the monitoring of early stage cancer.
Test Environment Construction Method Using Parameterized Test Environments (2017)

To achieve test automation, all stages such as the creation of test cases and data necessary to execute tests and the construction of test environments should be executed without any involvement of the tester. However, among the stages, the test environment construction process cannot be easily automated because it is executed through the tester's physical acts. To solve this problem, existing studies construct test environments using technologies such as virtualization or Infrastructure as Code. However, existing studies automate only a part of the test environment construction process. Therefore, this paper proposes the parameterized test environments, which are test environments expressed by parameter sets that characterize test environments. When this method is used, test environments can be constructed with only the inputs of parameters so that the problem of test environment construction, which was an obstacle to test automation, can be improved.
Improvement of HPMC Tablet Disintegration by the Addition of Inorganic Salts (2008)

Effects of inorganic salts on disintegration of hydroxypropylmethylcellulose (HPMC) matrix tablets have been studied. Adding disintegrants, such as Ac-di-sol®, Primojel®, Kolidon-CL®, or low substituted hydroxypropylcellulose (L-HPC®) to HPMC matrix tablets had no effect on disintegration property. Disintegration time was improved by adding NaHCO3, KH2PO4, K2SO4, KCl, or NaCl to the HPMC tablets as tablet components. On the other hand, addition of Na2CO3, or Na2SO4 to the tablets showed no improvement of disintegration. The heat of dissolution of inorganic salts that improved disintegration of tablets was endothermic, while that of inorganic salts that did not improve disintegration of tablets was exothermic. These results suggested that the thermal environment and ionic strength inside the tablet might affect the disintegration of HPMC matrix tablets.
Numerical characterisation of nonlinear stiffness properties of pre-stressed vibration isolation mounts (2002)

Numerical characterisation of nonlinear stiffness properties of pre-stressed vibration isolation mounts was presented. A computational procedure was developed to evaluate the condensed stiffness matrix of vibration isolators required by the vibration of viscoelastic and elastic structures (VVES) program. The procedure was based on a nonlinear finite element program which is able to handle complicated mount geometry and nonlinear effect associated with the static pre-loading. Three practical vibration isolation mount structures were analyzed and satisfactory numerical results were obtained.
Point of View (2010)

From the Division of General Medicine, Massachusetts General Hospital, Boston, MA. The manuscript submitted does not contain information about medical device(s)/drug(s). No funds were received in support of this work. No benefits in any form have been or will be received from a commercial party related directly or indirectly to the subject of this manuscript. Address correspondence and reprint requests to Steven J. Atlas, MD, Division of General Medicine, Massachusetts General Hospital, 50 Staniford Street, 9th Floor, Boston, MA 02114; E-mail: [email protected]