gebhart/patrepeval huggingface dataset:
Tumor Antigen–Specific FOXP3+ CD4 T Cells Identified in Human Metastatic Melanoma: Peptide Vaccination Results in Selective Expansion of Th1-like Counterparts (2009)

We have previously shown that vaccination of HLA-A2 metastatic melanoma patients with the analogue Melan-A(26-35(A27L)) peptide emulsified in a mineral oil induces ex vivo detectable specific CD8 T cells. These are further enhanced when a TLR9 agonist is codelivered in the same vaccine formulation. Interestingly, the same peptide can be efficiently recognized by HLA-DQ6-restricted CD4 T cells. We used HLA-DQ6 multimers to assess the specific CD4 T-cell response in both healthy individuals and melanoma patients. We report that the majority of melanoma patients carry high frequencies of naturally circulating HLA-DQ6-restricted Melan-A-specific CD4 T cells, a high proportion of which express FOXP3 and proliferate poorly in response to the cognate peptide. Upon vaccination, the relative frequency of multimer+ CD4 T cells did not change significantly. In contrast, we found a marked shift to FOXP3-negative CD4 T cells, accompanied by robust CD4 T-cell proliferation upon in vitro stimulation with cognate peptide. A concomitant reduction in TCR diversity was also observed. This is the first report on direct ex vivo identification of antigen-specific FOXP3+ T cells by multimer labeling in cancer patients and on the direct assessment of the impact of peptide vaccination on immunoregulatory T cells.
Epigenetic induction of adaptive immune response in multiple myeloma: sequential azacitidine and lenalidomide generate cancer testis antigen‐specific cellular immunity (2012)

Patients with multiple myeloma (MM) undergoing high dose therapy and autologous stem cell transplantation (SCT) remain at risk for disease progression. Induction of the expression of highly immunogenic cancer testis antigens (CTA) in malignant plasma cells in MM patients may trigger a protective immune response following SCT. We initiated a phase II clinical trial of the DNA hypomethylating agent, azacitidine (Aza) administered sequentially with lenalidomide (Rev) in patients with MM. Three cycles of Aza and Rev were administered and autologous lymphocytes were collected following the 2nd and 3rd cycles of Aza-Rev and cryopreserved. Subsequent stem cell mobilization was followed by high-dose melphalan and SCT. Autologous lymphocyte infusion (ALI) was performed in the second month following transplantation. Fourteen patients have completed the investigational therapy; autologous lymphocytes were collected from all of the patients. Thirteen patients have successfully completed SCT and 11 have undergone ALI. Six patients tested have demonstrated CTA up-regulation in either unfractionated bone marrow (n = 4) or CD138+ cells (n = 2). CTA (CTAG1B)-specific T cell response has been observed in all three patients tested and persists following SCT. Epigenetic induction of an adaptive immune response to cancer testis antigens is safe and feasible in MM patients undergoing SCT.
ひと目でわかるMicrosoft Windows 2000 Professional (2000)

Ｗｉｎｄｏｗｓ２０００ Ｐｒｏｆｅｓｓｉｏｎａｌの基本的な操作 必要な情報にすばやくアクセスする データを使いやすく整理する コンピュータ間でデータを共有する アプリケーションを使う ドキュメントを印刷する 音楽や映像を楽しむ 電子メール／ＦＡＸを利用する モバイルコンピューティングのために より使いやすくするために システムを管理する さらに詳細な設定を行う
Characterization of Interactions between the Transcriptional Repressor PhlF and Its Binding Site at the <i>phlA</i> Promoter in <i>Pseudomonas fluorescens</i> F113 (2002)

The phlACBD genes responsible for the biosynthesis of the antifungal metabolite 2,4-diacetylphloroglucinol (PHL) by the biocontrol strain Pseudomonas fluorescens F113 are regulated at the transcriptional level by the pathway-specific repressor PhlF. Strong evidence suggests that this regulation occurs mainly in the early logarithmic phase of growth. First, the expression of the phlF gene is relatively high between 3 and 13 h of growth and relatively low thereafter, with the phlACBD operon following an opposite expression profile. Second, the kinetics of PHL biosynthesis are specifically altered in the logarithmic phase in a P. fluorescens F113 phlF mutant. The phlA-phlF intergenic region presents a complex organization in that phlACBD is transcribed from a sigma(70) RNA polymerase-dependent promoter that is likely to overlap the promoter of the divergently transcribed phlF gene. The repression by PhlF is due to its interaction with an inverted repeated sequence, phO, located downstream of the phlA transcriptional start site. Cross-linking experiments indicate that PhlF can dimerize in solution, and thus PhlF may bind phO as a dimer or higher-order complex. Furthermore, it is now demonstrated that certain regulators of PHL synthesis act by modulating PhlF binding to phO. PHL, which has previously been shown to be an autoinducer of PHL biosynthesis, interacts with PhlF to destabilize the PhlF-phO complex. Conversely, the PhlF-phO complex is stabilized by the presence of salicylate, which has been shown to be an inhibitor of phlA expression.
Mechanisms for compile-time enforcement of security (1983)

This paper discusses features of a secure systems programming language designed and implemented at IBM's Watson Research Lab. Two features of the language design were instrumental in permitting security to be enforced with minimum run-time cost: (1) Language constructs (e.g. pointer variables) which could result in aliasing were removed from the programmer's direct control and replaced by higher level primitive types; and (2) traditional strong type checking was enhanced with typestate checking, a new mechanism in which the compiler guarantees that for all execution paths, the sequence of operations on each variable obeys a finite state grammar associated with that variable's type. Examples are given to illustrate the application of these mechanisms.
Methodology of reversible protein labeling for ratiometric fluorescent measurement (2005)

The first fluorescent labeling technology, which can induce not only an increase in the fluorescence intensity but also a shift in the fluorescence spectrum, has been developed for "ratiometric" measurements for a protein by utilizing a newly designed "field-sensitive" fluorescent probe and its corresponding unique amino acid tag.
Fabrication of gated SiC vertical edge emitters by chemical mechanical polishing (1997)

Sputtered SiC vertical edge emitters with a resistivity of cm, gate dimensions of 6, 9, 15, 25 and and an emitter-to-gate distance of have been fabricated using a combination of standard micromachining processes and chemical mechanical polishing (CMP). Electron emission propagates from high field points along the SiC periphery with very little restructuring of the emission sites. This fact and the scaleability of the CMP process offer the possibility for low-cost, large-area array implementation, suitable for display applications.
Preparation and Characterization of Mg Doped γ-Fe 2o3 Prepared by Self-Propagation Method (2014)

γ -Fe 2O3 doped Mg Nano particle is prepared by self-propaga tion method using polyethylene glycol a capping agent. A Nano particle was characterized by XRD (X-ray Diffraction), SEM (Scanning Electron Microscopey), VSM (Vibrating Sample Magnetometer), DSC (Differential Scanning Caloriemetry), and TGA (Thermal Gravimetric Analysis) to conform the structural, textural and magnetic property. Surface morp hology and size was confirmed by SEM. XRD analysis shows lattice parameter and crystal nature. VSM analysis shows that the sample has paramagnetic property. DSC analysis shows the number of characteristic prope rties of the sample. TGA analysis shows the phase transit ion of the sample. Keywords : Doping, γ -Fe 2O3, magnetic property, SEM, XRD, VSM.
Interaction of cells and nanofiber scaffolds in tissue engineering (2007)

Nanofibers and nanomaterials are potentially recent additions to materials in relation to tissue engineering (TE). TE is the regeneration of biological tissues through the use of cells, with the aid of supporting structures and biomolecules. Mimicking architecture of extracellular matrix is one of the challenges for TE. Biodegradable biopolymer nanofibers with controlled surface and internal molecular structures can be electrospun into mats with specific fiber arrangement and structural integrity for drug delivery and TE applications. The polymeric materials are widely accepted because of their ease of processability and amenability to provide a large variety of cost-effective materials, which help to enhance the comfort and quality of life in modern biomedical and industrial society. Today, nanotechnology and nanoscience approaches to scaffold design and functionalization are beginning to expand the market for drug delivery and TE is forming the basis for highly profitable niche within the industry. This review describes recent advances for fabrication of nanofiber scaffolds and interaction of cells in TE.
Regulating the Single-Mode Lasing Intensity of Semiconductor Lasers Without Wavelength Shift (2020)

A simple method to regulate the single-mode lasing intensity of semiconductor lasers without wavelength shift is demonstrated in this paper. The basic component is a digital micro-mirror device (DMD), which can be taken as an adjustable two-dimensional grating by its special structure. The lasing peak wavelength and peak intensity are respectively regulated by the DMD angular position and the DMD setting. The lasing peak wavelength could be tuned over by rotating the DMD. Meanwhile, the lasing peak intensity could be regulated by the DMD setting, which generates optical feedback digitally. The regulating speed is very high (up to 10 kHz) and has no wavelength shift in the lasing mode, which is more stable and faster than the conventional method.
Single drop impact onto liquid films: neck distortion, jetting, tiny bubble entrainment, and crown formation (1999)

Single drop impact onto liquid films is simulated numerically. Surface tension and gravity are taken into account, whereas viscosity and compressibility are neglected. This permits recourse to a boundary-integral method, based on an integral equation for a scalar velocity potential. Calculations are performed for normal impacts resulting in axisymmetric flows. For times that are small compared to the characteristic time of impact 2 R / w 0 ( R being the drop radius, w 0 its initial velocity towards the liquid film), it is found that a disk-like jet forms at the neck between the drop and the pre-existing liquid film, if the impact Weber number is high enough. This jet can pinch off a torus-shaped liquid volume at its tip or reconnect with the pre-existing liquid film, thus entraining a torus- shaped bubble. In reality, both the torus-shaped bubble and liquid torus will decay according to Rayleigh's capillary instability, thus breaking the cylindrical symmetry. This mechanism of bubble entrainment differs from those described in literature. For times that are comparable to or larger than the characteristic time of impact, capillary waves on the film, or the well-known crowns, are obtained again according to whether the impact Weber number is low or high enough.
Differences in quality between privately and publicly banked umbilical cord blood units: a pilot study of autologous cord blood infusion in children with acquired neurologic disorders (2010)

IV infusion of autologous CB is safe and feasible in young children with neurologic injuries. Quality parameters of privately banked CBUs are inferior to those stored in public banks. If efficacy of autologous CB is established clinically, the quality of autologous units should be held to the same standards as those stored in public banks.
Effect of Long-chain Branching on the Foaming of Polypropylene with Azodicarbonamide (2006)

In this article the influence of long-chain branching on the foaming behavior of polypropylene (PP) is investigated. Different branching contents are achieved by blending a linear PP (L-PP) and a long-chain branched PP (LCB-PP). Whereas, the L-PP does not exhibit any strain hardening in laboratory stretching experiments, blends with amounts of the LCB-PP higher than 2 wt% already show a pronounced strain-hardening behavior. The strain hardening increased with a growing amount of the long-chain branched PP. A laboratory scale foaming apparatus based on a capillary rheometer is developed for the foaming experiments. In foaming tests with azodicarbonamide as chemical blowing agent, a significant improvement of the foaming behavior with respect to a higher expansion ratio, a lower amount of connected cells, and a more homogeneous cell size distribution is found with increasing content of the LCB-PP up to a concentration of 50 wt%. At this concentration, the foaming behavior of the LCB-PP is reached. The results demonstrate that low amounts of long-chain branching can significantly improve the optimal foaming process of PPs with a chemical blowing agent, and that additions of the linear material up to 50 wt% to the LCB-PP do not have any influence on the favorable foaming performance of the long-chain branched PP.
Does the Degree of Preoperative Subluxation or Soft Tissue Tension Affect the Incidence of Postoperative Luxation in Dogs after Total Hip Replacement? (2010)

Luxation after canine THR is a multifactorial problem but preexisting subluxation/soft tissue laxity is a significant risk factor for this complication.
Diode array pumped kilowatt laser (1997)

The diode array pumped kilowatt laser (DAPKL) has demonstrated more than an order of magnitude increase in brightness and average power for short pulse diode-pumped solid-state lasers since its inception in 1991. Significant advances in component technology have been demonstrated, including: development of a diffusion bonding process for producing large slabs of Nd:YAG laser material. Phase conjugation by stimulated Brillouin scattering (SBS) has been demonstrated with high reflectivity and fidelity in a simple focused geometry with input powers of 100 W. Pulse energies at 1.06 /spl mu/m of 10 J have been demonstrated with a beam quality of 1.5 times diffraction limited at the 500-W level. An average power of 875 W at 100 Hz has been obtained. Efficient frequency doubling with a record power of 165 W has been demonstrated with 5 J per pulse at 0.53 /spl mu/m. Work is ongoing to enclose the system in a compact brassboard with improved performance and long term stability.
Enhanced CO<sub>2</sub> Solubility in Hybrid Adsorbents: Optimization of Solid Support and Solvent Properties for CO<sub>2</sub> Capture (2012)

In this study, we optimize the CO2 adsorption performance of hybrid adsorbents prepared by confining physical solvents in porous solid supports. A number of prospective solid supports and physical solvents are chosen to prepare hybrid adsorbents, and are subsequently evaluated in CO2 adsorption experiments. Generally, all the hybrid adsorbents show an enhancement of CO2 solubility compared to the bulk physical solvent. However, not all the adsorbents positively display an improvement in the CO2 adsorption performance as compared with the original solids after confining the physical solvent into the solids’ pore. The micropore blocking effect is observed in the impregnated forms of zeolite, activated carbon, silicagel, and cecagel. Furthermore, we have obtained certain requisites for a good solid support, as efficient structures should be mesoporous with large surface area. In addition, there is an optimized solvent’s size to achieve an optimized enhanced solubility. As a result, among the candidates, N-methyl-2-pyrrolidone confined in MCM-41 and alumina are identified as the most suitable hybrid adsorbents for an effective CO2-removal application.
Predicting Shot Locations in Tennis Using Spatiotemporal Data (2013)

Over the past decade, vision-based tracking systems have been successfully deployed in professional sports such as tennis and cricket for enhanced broadcast visualizations as well as aiding umpiring decisions. Despite the high-level of accuracy of the tracking systems and the sheer volume of spatiotemporal data they generate, the use of this high quality data for quantitative player performance and prediction has been lacking. In this paper, we present a method which predicts the location of a future shot based on the spatiotemporal parameters of the incoming shots (i.e. shot speed, location, angle and feet location) from such a vision system. Having the ability to accurately predict future short-term events has enormous implications in the area of automatic sports broadcasting in addition to coaching and commentary domains. Using Hawk-Eye data from the 2012 Australian Open Men's draw, we utilize a Dynamic Bayesian Network to model player behaviors and use an online model adaptation method to match the player's behavior to enhance shot predictability. To show the utility of our approach, we analyze the shot predictability of the top 3 players seeds in the tournament (Djokovic, Federer and Nadal) as they played the most amounts of games.
Synthesis of mesoporous silica materials (MCM‐41) using silica fume as the silica source in a binary surfactant system assisted by post‐hydrothermal treatment and its Pb<sup>2+</sup> removal properties (2016)

Abstract Mesoporous silica materials (MCM‐41) were successfully prepared by substituting silica fume for silicate in different surfactant systems. The effects of binary surfactant systems and post‐hydrothermal treatment on the morphology and structural properties of MCM‐41 were investigated based on X‐ray diffraction (XRD), N 2 sorption/desorption, and transmission electron microscopy analyses (TEM). The results confirmed that polyethylene glycol (PEG‐6000) is an effective additive template in a binary surfactant system for the synthesis of mesoporous silica materials. Meanwhile, the hydrothermal stability of the mesoporous silica materials can be improved by post‐hydrothermal treatment and the optimal experimental parameters are a temperature of 140 °C and a treatment time of 48 h. The formation of high‐quality MCM‐41 is based on the balance between the ordered assembly of the inorganic anionic species and cationic surfactant, in accordance with the electrostatic interactions and hydrogen bonds between PEG‐6000 and the inorganic species. Meanwhile, Pb(II) removal from aqueous solution has also been examined using MCM‐41 as an adsorbent. The results of adsorption showed that the as‐synthesized MCM‐41 demonstrated a high capacity of Pb 2+ .
Tri-layer a-Si:H integrated circuits on polymeric substrates (2002)

Using a thermal mountant, we have fabricated hydrogenated amorphous silicon (a-Si:H) thin film transistors (TFTs) and integrated circuits on both colored and nearly colorless polyimide substrates with performance very similar to devices fabricated on glass substrates. Delay and power dissipation were measured with ring oscillators; minimum stage delay was less than 10 /spl mu/sec, and minimum power dissipation was less than 10 /spl mu/W per stage. These results indicate that with suitable thermal engineering, a-Si:H devices and circuits can be fabricated on polymeric substrates using nearly standard processing.
Mechanism of the metal-mediated endocytosis of the prion protein (2008)

The cellular form of the prion protein, PrP(c), is critically required for the establishment of prion diseases, such as Creutzfeldt-Jakob disease. Within the N-terminal half of PrP(c) are four octapeptide repeats that bind Cu(2+). Exposure of neuronal cells expressing PrP(c) to Cu(2+) results in the rapid endocytosis of the protein. First, PrP(c) translocates laterally out of detergent-resistant lipid rafts into detergent-soluble regions of the plasma membrane, then it is internalized through clathrin-coated pits. The extreme N-terminal region of PrP(c) is critically required for its endocytosis, as is the transmembrane LRP1 (low-density lipoprotein receptor-related protein-1). Incubation of cells with a competitive inhibitor of LRP1 ligands, receptor-associated protein, or down-regulation of LRP1 with siRNA (short interfering RNA) reduces the endocytosis of PrP(c). Zn(2+) also promotes the endocytosis of PrP(c), a phenomenon that is also dependent on the octapeptide repeats and requires LRP1.
A digit-serial algorithm for the discrete logarithm modulo 2/sup k/ (2004)

We introduce as our main result a digit-serial residue arithmetic algorithm for computing the discrete logarithm modulo 2/sup k/ (dlg). Digit inheritance is presented as a fundamental property common to the primitive operations modulo 2/sup k/ of addition, multiplication, multiplicative inverse, exponentiation and discrete logarithm. Our main algorithm computes dlg using binary arithmetic with 3 as the logarithmic base and has a critical path containing one modulo 2/sup k/ multiplication operation for each of its k iterations. Extensions of the algorithm to other logarithmic bases and computations using digits in a higher radix 2/sup r/ are also described.
Extreem rechts en de socialistische partij: hun sociologische relatie van het interbellum tot nu (2003)

Stem cell behaviours, such as stabilization of the undecided state of pluripotency or multipotency, the priming towards a prospective fate, binary fate decisions and irreversible commitment, must all somehow emerge from a genome-wide gene-regulatory network. Its unfathomable complexity defies the standard mode of explanation that is deeply rooted in molecular biology thinking: the reduction of observables to linear deterministic molecular pathways that are tacitly taken as chains of causation. Such culture of proximate explanation that uses qualitative arguments, simple arrow-arrow schemes or metaphors persists despite the ceaseless accumulation of 'omics' data and the rise of systems biology that now offers precise conceptual tools to explain emergent cell behaviours from gene networks. To facilitate the embrace of the principles of physics and mathematics that underlie such systems and help to bridge the gap between the formal description of theorists and the intuition of experimental biologists, we discuss in qualitative terms three perspectives outside the realm of their familiar linear-deterministic view: (i) state space (ii), high-dimensionality and (iii) heterogeneity. These concepts jointly offer a new vista on stem cell regulation that naturally explains many novel, counterintuitive observations and their inherent inevitability, obviating the need for ad hoc explanations of their existence based on natural selection. Hopefully, this expanded view will stimulate novel experimental designs.
P‐6: 4‐side Micro Border 8k4k LCD with Oxide‐TFT Gate Driver Embedded Array (2020)

We have realized the 4‐side micro (0.9x/0.9x/0.9x/0.9x mm) border 8k4k 120Hz LCD with Oxide thin‐film transistor (TFT) for the first time. Most of narrow border prototypes in past was 3‐side with 2‐COF process for both gate and data driving, but now, we have been developing the most cost effective 4‐side micro border LCD with only data COF. As core technology, 1G1D pixel driving and GDCIAA (Gate Driver Circuit in Active Area) with oxide TFT backplane are incorporated without any additional investment of module assembly. In practical point of view, this technology can render about 70% reduction of D‐IC cost and additional value up as a form factor of premium product compare to a‐Si TFT based LCD. Moreover, simplified 1G1D bus line structure can contribute to production yield improvement. In this report, we present the most valuable 8k4k LCD product with the latest AUOs Oxide TFT backplane technology.
GaAs MMIC tunable directional coupler (2012)

This paper presents a tunable GaAs MMIC directional coupler. Design equations for a hybrid coupler with ideal tunable capacitances are derived to determine the range of capacitances for the desired coupling tuning range. The 2GHz coupler is implemented in a 0.5 µm GaAs process with Schottky varactor diodes, and occupies an area of 2.2mm×1.4 mm. Over a bias range of 0V to −1.5V the coupling coefficient ranges from 6.6 to 60 dB, or 53.4 dB of dynamic range at 2 GHz. The input return loss for the device is better than 12 dB for all bias voltages and is better than 20 dB for coupling coefficients from 23 to 60 dB with isolation better than 17 dB.