gebhart/patrepeval huggingface dataset:
Sleep homeostasis in the rat is preserved during chronic sleep restriction (2010)

Sleep is homeostatically regulated in all animal species that have been carefully studied so far. The best characterized marker of sleep homeostasis is slow wave activity (SWA), the EEG power between 0.5 and 4 Hz during nonrapid eye movement (NREM) sleep. SWA reflects the accumulation of sleep pressure as a function of duration and/or intensity of prior wake: it increases after spontaneous wake and short-term (3-24 h) sleep deprivation and decreases during sleep. However, recent evidence suggests that during chronic sleep restriction (SR) sleep may be regulated by both allostatic and homeostatic mechanisms. Here, we performed continuous, almost completely artifact-free EEG recordings from frontal, parietal, and occipital cortex in freely moving rats (n = 11) during and after 5 d of SR. During SR, rats were allowed to sleep during the first 4 h of the light period (4S(+)) but not during the following 20 h (20S(-)). During the daily 20S(-) most sleep was prevented, whereas the number of short (<20 s) sleep attempts increased. Low-frequency EEG power (1-6 Hz) in both sleep and wake also increased during 20S(-), most notably in the occipital cortex. In all animals NREM SWA increased above baseline levels during the 4S(+) periods and in post-SR recovery. The SWA increase was more pronounced in frontal cortex, and its magnitude was determined by the efficiency of SR. Analysis of cumulative slow wave energy demonstrated that the loss of SWA during SR was compensated by the end of the second recovery day. Thus, the homeostatic regulation of sleep is preserved under conditions of chronic SR.
Chemical mechanical polishing of thin film diamond (2013)

The demonstration that Nanocrystalline Diamond (NCD) can retain the superior Young’s modulus (1100 GPa) of single crystal diamond twinned with its ability to be grown at low temperatures (<450 °C) has driven a revival into the growth and applications of NCD thin films. However, owing to the competitive growth of crystals the resulting film has a roughness that evolves with film thickness, preventing NCD films from reaching their full potential in devices where a smooth film is required. To reduce this roughness, films have been polished using Chemical Mechanical Polishing (CMP). A Logitech Tribo CMP tool equipped with a polyurethane/polyester polishing cloth and an alkaline colloidal silica polishing fluid has been used to polish NCD films. The resulting films have been characterised with Atomic Force Microscopy, Scanning Electron Microscopy and X-ray Photoelectron Spectroscopy. Root mean square roughness values have been reduced from 18.3 nm to 1.7 nm over 25 μm2, with roughness values as low as 0.42 nm over ∼0.25 μm2. A polishing mechanism of wet oxidation of the surface, attachment of silica particles and subsequent shearing away of carbon has also been proposed.
The ESCRT System Is Required for Hepatitis C Virus Production (2011)

These results suggest that the ESCRT system is required for infectious HCV production.
Inverse design-momentum, a method for the preliminary design of horizontal axis wind turbines (2007)

Wind turbine rotor prediction methods based on generalized momentum theory BEM routinely used in industry and vortex wake methods demand the use of airfoil tabulated data and geometrical specifications such as the blade spanwise chord distribution. They belong to the category of "direct design" methods. When, on the other hand, the geometry is deduced from some design objective, we refer to "inverse design" methods. This paper presents a method for the preliminary design of wind turbine rotors based on an inverse design approach. For this purpose, a generalized theory was developed without using classical tools such as BEM. Instead, it uses a simplified meridional flow analysis of axial turbomachines and is based on the assumption that knowing the vortex distribution and appropriate boundary conditions is tantamount to knowing the velocity distribution. The simple conservation properties of the vortex components consistently cope with the forces and specific work exchange expressions through the rotor. The method allows for rotor arbitrarily radial load distribution and includes the wake rotation and expansion. Radial pressure gradient is considered in the wake. The capability of the model is demonstrated first by a comparison with the classical actuator disk theory in investigating the consistency of the flow field, then the model is used to predict the blade planform of a commercial wind turbine. Based on these validations, the authors postulate the use of a different vortex distribution (i.e. not-uniform loading) for blade design and discuss the effect of such choices on blade chord and twist, force distribution and power coefficient. In addition to the method's straightforward application to the pre-design phase, the model clearly shows the link between blade geometry and performance allowing quick preliminary evaluation of non uniform loading on blade structural characteristics.
Simplifying biodiesel production: The direct or in situ transesterification of algal biomass (2011)

Abstract The in situ esterification/transesterification of algal biomass lipids to produce FAME for potential use as biodiesel was investigated. Commercial algal biomass was employed, containing 20.9 wt% hexane extractable oil. This consisted of 35.1 wt% free fatty acids (FFA), 18.2 wt% TAG, and 8.8 wt% MAG, accounting for 62.1% of the extractable material. Other constituents of the hexane extractable material, accounting for 37.9% of the extracts, were not further characterized. The predominant fatty acids in the oil were palmitic (42.4 wt%), oleic (30.6 wt%), linoleic (22.8 wt%), and linolenic (16.1 wt%). Small amounts of 10‐keto 16:0 and 10‐OH 16:0 fatty acids were also present. Statistical experimental design was employed to coordinately examine the effects of the amounts of methanol, sulfuric acid, and reaction temperature (23–65°C) on the yield of FAME in 2 h reactions. Three methods of feedstock preparation were examined – as received, oven dried, and water‐washed/dried. For all feedstocks conditions could be identified which were predicted to yield greater than 90% maximum theoretical FAME production. Oven drying the feedstock reduced the amount of methanol required, with 83% of maximum yield obtained at a methanol/fatty acid molar ratio of 220:1 (4 mL methanol/g substrate). Water washing the biomass did not reduce the methanol required for high level transesterification. Practical applications : In biodiesel production by the conventional method of alkali‐catalyzed transesterification of a refined vegetable oil/the combination of feedstock and process costs threatens the economic viability of biofuel production. In situ esterification/transesterification is a method for producing biodiesel wherein a lipid‐bearing material is directly treated with reagents that catalyze fatty acid alkyl ester production from FFA and acylglycerols. By eliminating the need to isolate and refine the feedstock lipid, this approach to biodiesel production eliminates some of the processing steps required by contemporary methods. This could provide a welcome reduction in the cost of biodiesel production. There is presently considerable interest in the possibility of biofuel production from algal biomass. This study describes the application and optimization of in situ transesterification to a lipid‐bearing algal biomass. Application of this technology could facilitate the economical production of biodiesel from algal biomass.
Review of superconducting transition-edge sensors for x-ray and gamma-ray spectroscopy (2015)

We present a review of emerging x-ray and gamma-ray spectrometers based on arrays of superconducting transition-edge sensors (TESs). Special attention will be given to recent progress in TES applications and in understanding TES physics.
Characterization of the activation-associated isoform of CD43 on murine T lymphocytes. (1994)

A rat mAb termed 1B11 recognizes a 130-kDa cell surface glycoprotein expressed on T lymphocytes. Transfection studies using the Cd43 gene transfected into murine L cells, and immunoblots using anti-peptide Abs specific for the CD43 polypeptide identified the 1B11 Ag as the 130-kDa isoform of murine CD43. mAb 1B11 fails to recognize the other major CD43 isoform, 115-kDa CD43, either by Western blotting or by FACS analysis, thus differing from the previously characterized anti-CD43 mAb S7 that recognizes only the CD43 115-kDa isoform and not the CD43 130-kDa isoform. CD43 130-kDa recognized by mAb 1B11 is differentially expressed on T lymphocytes. Whereas most CD4-8-, CD4+8+, and CD4-8+ thymocytes express 130-kDa CD43 constitutively, the Ag is expressed by less than 20% of CD4+ T cells in immature and mature populations. On activation, expression of 130-kDa CD43 is up-regulated dramatically on CD4+ T lymphocytes, and to a lesser extent on CD8+ T lymphocytes. In contrast, T cell activation resulted in only minor up-regulation of 115-kDa CD43. CD43 130-kDa contains sialylated O-linked carbohydrate; however, recognition by mAb 1B11 is not dependent on the presence of sialic acid. Interestingly, removal of sialic acid by neuraminidase treatment of 1B11-negative CD4+ T lymphocytes or 1B11-negative EL4 cells confers 1B11 reactivity, suggesting that the 1B11 epitope is masked by sialic acid residues on the CD43 115-kDa isoform. The isoelectric point (pl) of 130-kDa CD43 was determined to be 6.0, which is higher than the pl reported for 115-kDa CD43. Different molecular properties of 115-kDa and 130-kDa CD43 and their differential expression in T cell subsets may indicate specific roles for these CD43 isoforms in T cell ontogeny and/or T cell function.
Ultra-low phase noise superconducting-cavity stabilised microwave oscillator with application to gravitational radiation detection (1983)

A superconducting-cavity stabilised oscillator has been constructed which combines active and passive stabilisation of a 9.6 GHz klystron to obtain a phase noise power spectral density between -170 dB Hz-1 and -180 dB Hz-1 at 5 kHz from the carrier, which represents about 40 dB improvement over the state-of-the-art. The device has been developed for use as a low noise pump for a superconducting parametric upconverter transducer on a gravitational radiation antenna. An RF modulated reflex klystron is actively locked to a TE011 niobium cavity operated at 2K with a loaded Q approximately 108. Power is extracted from the cavity which acts as a high Q filter.
Polypharmacy and Adverse Drug Events Leading to Acute Care Hospitalization in Japanese Elderly (2014)

Background: Elderly patients are considered to be at risk of developing adverse drug events (ADEs) because they tend to receive a greater number of medications. The purpose of our study is to determine the prevalence of ADEs related to polypharmacy and causative admissions of patients in Japanese acute care hospitalization. Methods: In retrospective cohort study, we analyzed 700 consecutive elderly patients admitted to the department of medicine of a Japanese community hospital in 2011. ADEs were defined by World Health Organization–Uppsala Monitoring Centre criteria. Polypharmacy was defined as five or more medications. Results: The mean age was 79.5 years (men, 54%). The mean number of medications was 6.36 +/− 4.15 (maximum, 26). Polypharmacy was observed in 63% of cases. ADEs were identified in 4.9% (95% CI, 3.5–6.7%). The mean numbers of medications among patients with ADEs and those without ADEs were 9.3 +/− 3.4 and 6.2 +/− 4.1, respectively. A greater number of medications was significantly associated with ADEs (p < 0.001). Polypharmacy was identified in 91% of patients with ADEs, while it was noted in 62% of patients without ADEs (p = 0.001). Using logistic regression analysis, polypharmacy was significantly associated with ADEs (Odds ratio 5.89, 95% CI 1.74–19.9). The highest number of ADEs were identified among patients on antiplatelets or anticoagulants (n = 8), followed by benzodiazepines and NSAIDs (n = 4 for both). The most common ADEs were gastrointestinal bleeding, nausea and congestive heart failure. Conclusion: ADEs complicated to about 5% of acute care elderly hospitalizations in Japan. Polypharmacy was significantly associated with these ADEs.
Single-shot phase-shifting color digital holography (2007)

Use of phase-shifting interferometry in digital holography allows direct detection of the complex amplitude at the image sensor and achieves complete complex amplitude of the images, in general, it requires three or more holograms to reconstruct a single image of the complex amplitude. In this paper we propose single-shot phase-shifting color digital holography capable of instantaneous three-dimensional color measurement and data acquisition.
Autonomous Animation and Control of Four- Legged Animals (1995)

Autonomous Animation and Control of Four-Legged Animals This paper develops a framework for the realistic autonomous animation and motion control of four-legged animals. Our system uses a combination of kinematics, dynamics and control theory. The input to our system, the velocity and heading of the animal, originates either from a simulated visual sensory system or a user. Based on this input we model walking, trotting, and simple behaviors such as target pursuit. we use a combination of kinematics, dynamics and control theory. A feedback controller, using the desired velocity and animal heading, computes the aggregate force and torque vector that should be applied to the body to achieve the given motion. This force and torque is distributed to the legs in contact with the ground through a linear programming algorithm. We then use forward dynamics to compute the actual body displacement. A kinematic gait controller is in charge of the stepping pattern. It arranges the stance and transfer phases of each leg depending on the current locomotion velocity, the turning rate and the ground conditions. Although we chose to focus on four-legged animals, the approach is generalizable to other multi-legged creatures or biped locomotion. Our motion system can currently simulate variable speed walking and trotting on flat or uneven terrain. Given its flexibility, the system can be used as a basis for more complex animations involving high level behaviors and interactions with other animals.
Physico‐chemical properties of crystalline nanoscale enzyme‐protein‐metal layer composites in biosensors (1997)

Abstract Crystalline bacterial cell surface proteins (S‐layers) are introduced as immobilization matrices for binding biologically functional molecules in the development of amperometric biosensors. Due to the crystalline character of S‐layers, functional groups are located in well defined position and orientation on the protein meshwork and thus allow to bind molecules in densely packed monolayers. One of the most critical procedures in the development of amperometric biosensors is the deposition of an electrical contact onto the sensing layer. Conventional, sputtering techniques using platinum or gold, or, more recently, pulsed laser deposition of precious metals under inert gas conditions allow to establish electrically conductive contacts on S‐layer‐based enzyme electrodes.
Quantification of organic acids in grape musts and Port wines (2002)

"A simple, rapid and accurate method for derivatization and quantitative HPLC analysis of organic acids, was optimised inorder to provide the determination of the most important acids found in Port wines and grape musts used for their elaboration. Themethod was based on the use of O-(4-nitrobenzyl)-N,N´-diisopropylisourea (NBDI) as derivatizing reagent. Samples were primarilytreated 15 min with a strong cation-exchange resin (Dowex 50W-X8) to free the organic acids. HPLC determination was accomplishedby using a RP-18 (3 µm) column, a mobile phase composed of water and acetonitrile (non-linear gradient) at a flow-rate of 1 mL min-1, and a UV detector set at 265 nm. Sensitive and well-resolved peaks were obtained from the main organic acids (lactic, acetic,succinic, tartaric, malic and citric acids) in less than 30 min, either with Port wines or grape musts. Benzylmalonic acid was used asinternal standard. Precision and recovery assays were performed with good results for all of 6 acids considered. The major differencesbetween grape musts and Port wines were the greater overall amounts of organic acids in the former and the higher content of lactic,acetic and succinic acids in the latter. © 2002 Altaga. All rights reserved."
Synthesis of Indolo[3,2-<i>b</i>]carbazole-Based Boron Complexes with Tunable Photophysical and Electrochemical Properties (2018)

New synthetic strategies were developed for the synthesis of indolo-pyridine boron difluoride (IPBD) dyes and antiladder-type π-conjugated dyes based on the pyridine/pyrazine-indolocarbazole (ICZ) structures. The photophysical and electrochemical properties of the dyes were measured in solution, solid state, and thin films and rationalized by theoretical calculations. Interestingly, these properties of the dyes can be tuned in a wide range using the developed chemical route. For example, the absorption range and fluorescence color of the dyes in solution and solid state and the HOMO-LUMO energy gaps were tuned by structure modulations. The absorption properties of those new boron complexes cover most of the UV-visible-NIR spectrum. Therefore, this appealing tunability feature makes these new types of dyes very promising candidates for their further use in functional material development.
Three-Way-Catalyst Modeling - A Comparison of 1D and 2D Simulations (2007)

<div class="htmlview paragraph">In this paper we present a comparison of two different approaches to model three-way catalyst. First, a numerical sample case simulating light-off is used to compare the 1D and the 2D models. The advantages of each code are discussed with respect to required input data, detail level of the output, comparability, and computation time. Thus, the 2D model reveals significant radial temperature gradients inside the monolith during light-off.</div> <div class="htmlview paragraph">In a second step, the 2D model is compared with experimental data. One set of data consists of an air/fuel ratio varying sweep at isothermal conditions. Another set was gained by emission measurements during a real driving MVEG tests with varying substrate cell density &amp; inlet conditions. From these experiments the applicability of the model to numerical parameter studies is discussed.</div>
The synergistic effect of microwave drying and plasma surface treatments on the wettability of green wood (2022)

In spite of being both a one-step solution to several problems associated with woodworking and also energy efficient, the application of microwave (MW) modification in wood research has remained very limited and this promising method has practically no use in wood industries across the globe. Research done so far in this field primarily sheds light on its potential in enhancing wood permeability, treatability and uniform wood drying. While MW treatments are mostly used on wet or green wood, another modification technique, plasma, has potential benefits to synergistically enhance the effects of MW treatment, but so far has not been applied on wet or green wood specimens. This study takes a first step to investigate the effects of plasma treatments (PT) on green wood specimens, as well as combinations of MW and plasma treatments. As a preliminary study, the methodology focuses on water contact angle measurements, since these are most commonly used as indicators for surface modifications in industrial applications. An exponential time dependence was found for the contact angle on the investigated samples of Norway spruce (Picea abies Karst.). Initial contact angles after droplet deposition increased due to drying and migration of organic molecules during treatments. In comparison with the literature, the effect of plasma was significantly less pronounced on wet wood specimens. The initial contact angles showed the lowest statistical variations after MW treatment, whereas plasma increased inhomogeneities. The final contact angles on treated specimens was lowest for PT-only specimens as well as specimens treated with plasma after MW. In contrast to the initial contact angles, the final contact angles showed the lowest variations after PT. Wetting rates were insignificantly improved by plasma, with reduced statistical variations after all treatments.
Circular mRNA Encoding for Monomeric and Polymeric Green Fluorescent Protein (2003)

Many proteins with unusual structural properties are comprised of multiple repeating amino acid sequences, and are often fractious to expression in recombinant systems. To facilitate recombinant production of such proteins for structural and engineering studies, the author has developed a method for producing messenger RNAs on circular RNA templates. This circularization process is derived from a rearranged group I intron, from which circular RNA is produced through the splicing activity of autocatalytic group I RNA elements (Fig. 1; 1,2). Because the only cofactors required for splicing of the group I intron are magnesium and guanosine, the process can take place in a variety of organisms, making it amenable to a wide variety of protein expression systems (1–4).
Optical attenuation in defect-engineered silicon rib waveguides (2006)

The excess optical attenuation at wavelengths around 1550nm induced by subamorphous dose ion implantation of silicon-on-insulator rib waveguides has been quantified. Optical attenuation is related to the introduction of lattice defects such as the silicon divacancy. After 2.8MeV Si+ implantation at a dose of 2.5×1014cm−2, the attenuation is greater than 1000dBcm−1. Using positron annihilation spectroscopy to determine the vacancy-type defect concentration, it is demonstrated that the absorption component of the excess attenuation can be predicted using a simple analytical expression. Additional losses are suggested to result from a defect induced change in the real part of the refractive index of the silicon waveguide. A processing strategy for ensuring that the absorption component dominates the excess attenuation is described, and it is shown that selective implantation of a relatively low dose of inert ions is an efficient method for the reduction of optical cross talk in silicon photonic circuits.
Unique Lipid A Modifications in<i>Pseudomonas aeruginosa</i>Isolated from the Airways of Patients with Cystic Fibrosis (2007)

Three structural features of lipid A (addition of palmitate [C16 fatty acid], addition of aminoarabinose [positively charged amino sugar residue], and retention of 3-hydroxydecanoate [3-OH C10 fatty acid]) were determined for Pseudomonas aeruginosa isolates from patients with cystic fibrosis (CF; n=86), from the environment (n=13), and from patients with other conditions (n=14). Among P. aeruginosa CF isolates, 100% had lipid A with palmitate, 24.6% with aminoarabinose, and 33.3% retained 3-hydroxydecanoate. None of the isolates from the environment or from patients with other conditions displayed these modifications. These results indicate that unique lipid A modifications occur in clinical P. aeruginosa CF isolates.