gebhart/patrepeval huggingface dataset:
DNA replication from initiation zones of mammalian cells in a model system. (1994)

We reported that DNA replication initiates from the region containing an autonomously replicating sequence from Saccharomyces cerevisiae when negatively supercoiled plasmid DNA is incubated with the proteins required for simian virus 40 DNA replication (Y. Ishimi and K. Matsumoto, Proc. Natl. Acad. Sci. USA 90:5399-5403, 1993). In this study, the DNAs containing initiation zones from mammalian cells were replicated in this model system. When negatively supercoiled DNA containing an initiation zone (2 kb) upstream of the human c-myc gene was incubated with simian virus 40 T antigen as a DNA helicase, HSSB (also called replication protein A), and DNA polymerase alpha-primase complex isolated from HeLa cells, DNA replication was specifically initiated from the center of the initiation zone, which was elongated bidirectionally in the presence of a DNA swivelase. Without HSSB, the level of DNA synthesis was significantly reduced and the localized initiation could not be detected, indicating that HSSB plays an essential role in the initiation of DNA replication. The digestion of negatively supercoiled template DNA with a single-strand-specific nuclease revealed that HSSB stimulated DNA unwinding in the center of the initiation zone where the DNA duplex is relatively unstable. In contrast, DNA replication started from a broad region of an initiation zone downstream of the dihydrofolate reductase gene from chinese hamster ovary cells, but the center of the region was mapped near the origin of bidirectional DNA replication. These results suggested that this system mimics a fundamental process of initiation of eukaryotic DNA replication. The mechanism of initiation is discussed.
Influence of baseline variables on changes in <scp>I</scp> nternational <scp>P</scp> rostate <scp>S</scp> ymptom <scp>S</scp> core after combined therapy with dutasteride plus tamsulosin or either monotherapy in patients with benign prostatic hyperplasia and lower urinary tract symptoms: 4‐year results of the <scp>CombAT</scp> study (2013)

CombAT data support the use of long-term combination therapy with dutasteride and tamsulosin in patients considered at risk for progression of BPH, as determined by high PV (≥30 mL) and high PSA (≥1.5 ng/mL). Combination therapy, dutasteride monotherapy and tamsulosin monotherapy all improved Qmax , but to different extents (combination therapy > dutasteride >> tamsulosin), suggesting that dutasteride contributes most to the Qmax benefit in combination therapy. Combination therapy provided consistent improvement over tamsulosin in LUTS across all analysed baseline variables at 48 months. Compared with dutasteride, the superiority of combination therapy at 48 months was shown in patients with PV <60 mL or PSA <4 ng/mL.
Expression of Recombinant G-Protein Coupled Receptors for Structural Biology (2007)

A graphical abstract is available for this content
MALDI-TOF MS based carbapenemase detection from culture isolates and from positive blood culture vials (2016)

This MALDI-TOF MS-based assay permitted the detection of carbapenemases either from solid culture media (98-100 %) or blood culture vials (96 %) for all non-A. baumannii isolates within 4 h. In case of A. baumannii isolates the assay was highly sensitive for the detection of carbapenemases directly from solid culture media.
<i>mda</i>-9/Syntenin: More than Just a Simple Adapter Protein When It Comes to Cancer Metastasis (2008)

Cancer is a progressive disease that, in many instances, if untreated, can culminate in metastatic spread of primary tumor cells to distant sites in the body. Metastasis frequently confers virulence and therapy resistance to cancer cells, and defining the molecular events that control metastasis will be mandatory to develop rational, targeted therapies for effective intervention, prevention of recurrence, and the "holy grail" of engendering a cure. Adapter proteins are physiologically pertinent molecules that, through interactions with key regulatory proteins via specific conserved domains, control important cellular events. Melanoma differentiation associated gene-9 (mda-9), also known as syntenin, is a PDZ domain-containing adapter protein that is involved in organization of protein complexes in the plasma membranes, regulation of B-cell development, intracellular trafficking and cell-surface targeting, synaptic transmission, and axonal outgrowth. Recent studies now define a seminal role for mda-9/syntenin in cancer metastasis. The present review provides a current perspective of our understanding of this important aspect of mda-9/syntenin, suggesting that this gene and its encoded protein and interacting protein partners may provide viable targets for intervening in the final and invariably the most lethal stage of cancer progression, namely, cancer metastasis.
Thermoelectric performance of PbSe quantum dot films (2013)

The thermoelectric (TE) performance of films of colloidal lead selenide (PbSe) quantum dots (QDs) with metal-chalcogenide complex ligands is seen to change with QD size and temperature. Films of smaller QDs have higher Seebeck coefficient magnitudes, indicating stronger quantum confinement, and lower electrical and thermal conductivities. The thermoelectric figure of merit ZT is ∼0.5 at room temperature and increases with temperature to 1.0–1.37 at ∼400 K, where it is larger for smaller QD films. This is better than previous results for solution-prepared QD TE materials at these elevated temperatures.
Process technologies for high density, high speed 16 megabit dynamic RAM (1987)

This paper describes key points of submicron CMOS technologies for an experimental 16 Mbit DRAM fabrication. The memory cell and the transistor designs are most important to realize high density, high speed DRAMs. The main features of the technology are a new buried stacked capacitor cell and a high speed CMOS structure. The lithographic levels used were 0.7 µm for critical layers. The technologies have been verified using test vehicles and experimental 16 Mbit DRAM.
Nanowire Heterostructures (2013)

The nanoscale diameter and high aspect ratio of nanowires are the foundation of fascinating structure-property relationships derived from confinement, interface effects, and mechanical degrees of freedom. When heterostructures are formed by high-quality growth of dissimilar materials on or within nanowires, the interactions of the low-dimensional components and their interfaces can give rise to electronic, photonic, magnetic, and thermal characteristics that are superior to those of (or unattainable in) planar geometries. This tutorial review provides a brief overview of heterostructures with a semiconductor nanowire as the central component, describes the properties of nanoscale components and interfaces, and distills the advantages that arise from the unique structure-property relationships. A select set of these concepts are further elaborated by highlighting electronic, optoelectronic, and energy-related applications that have successfully exploited these advantages.
Performance Study of an Air-Oil Pump and Separator Solution (2012)

Many applications need to extract a certain phase from a multiphase flow like in oil extraction, flow in nuclear power plants, aircraft lubrication systems, etc. The Aero-Thermo-Mechanics (ATM) Department of Universiteé Libre de Bruxelles (ULB) is developing an original system to extract the gas from a liquid-gas flow together with increasing the pressure of the liquid phase. This system will help to reduce the complexity and the oil consumption of aeroengine lubrication systems. This paper will summarise the results of a first air/oil prototype. It will also present the guidelines learned from this prototype and used to design a second version of the integrated pump and separator. A newly developed oil consumption measurement system will also be presented. Based on previous results, on litterature review and on an in-house theoretical model, the paper will explain theoretically how the separation efficiency is affected by the particle distribution at the inlet of the prototype, and by the key parameters identified in different studies. Finally the conclusions will present the lessons learned through the design and tests of these two prototypes and the future work will be presented.
A Distributed Algorithm for Service Commitment in Allocating Services to Applications (2007)

In this paper, we extend our previous work on committing service instances to the applications in a service-oriented environment, and we propose a distributed heuristic algorithm which is able to estimate the number of future service instances needed by each application instance in a future time. Also, this algorithm does not assume any specific type of distribution function for services execution time and applications interarrival time. In this paper, after presenting the mathematical basis for the proposed distributed algorithm, we explain how this algorithm can be implemented in a distributed environment, and through the simulations and performance comparisons, we show that the proposed algorithm improves the performance of the system significantly, compared to a No commitment policy system and a full commitment policy system.
A Monitoring System for Machine Learning Models in a Large-Scale Context (2020)

Since building a machine learning model costs a lot while following 9 stages, the automated machine learning model creation became a crucial role in a large-scale context. At the same time, a monitoring system became an essential factor for machine learning models. This thesis presents the monitoring system for machine learning models at ING in an enterprise context with new features required by users. Moreover, the thesis describes a case study of ING, a large global banking company that develops software solutions in-house. We conducted a mixed-methods study, consisting of data collection of the monitoring system and a survey with the users of the monitoring system. Our research shows that challenges found by the actual users of the monitoring system and mapped challenges discovered by the Microsoft study are related to machine learning model monitoring, the perception of the users on the importance of the monitoring system, and the impact of the monitoring system. We found that the monitoring system at ING supports relatively efficient model management in terms of checking model validation and evaluation. Moreover, the users of the monitoring system perceived that it is an important system, and it supports the models regarding quality, the trust of the automated model creation, and usability. Additionally, compared to the existing solution, the monitoring system at ING supports useful model management.
Novel Applications Possibilities for Phase-Change Materials and Devices (2013)

Phase-change materials and devices are most widely known for their use in optical and electrical non-volatile memory applications. Recently however the potential has been demonstrated for using phase-change materials and devices for a range of novel applications, including the provision of electronic 'mimics' of biological synapses and neurons (and their associated use in neuromorphic computing) and the provision of arithmetic and logic functionality. Furthermore, such neuromorphic, arithmetic and logic capabilities of phase-change materials and devices are accessible in both the optical (photonic) and the electrical (electronic) domains, or indeed via a 'mixed-mode' approach in which excitation is in the optical domain and detection is electrical, or vice-versa. This versatility of operation opens up the route towards various intriguing possibilities, such as 'all-optical' memory and computing devices, or the development of an optical analogue of the memristor, the so-called 'memflector'. In this paper we discuss such novel applications possibilities for phase-change materials and devices and present proof-of-principle of some of the underlying concepts.
“Municipal ‘diversity’ and ‘immigration’ policymaking in Canada: a comparative research agenda and call for the empowerment of municipalities” (2019)

Beginning in the early 1990s studies showing that some municipal governments had begun to take a role in ‘multiculturalism’ policymaking (a contested term at the local level) emerged first in Toronto, Montreal and Vancouver, Canada’s most significant immigrant destinations (Tate and Quesnel 1995; Wallace and Frisken 2000; Edgington and Hutton 2002). This literature establishes that municipalities vary in two major ways ...
Fault tolerant mechanisms for SDN controllers (2016)

Software-Defined Networking (SDN) enables decoupling of the data plane from the control plane on network devices. This approach differs from the traditional networking architecture where both planes reside on the same network device. SDN offers flexibility, centralized control, reduced complexity and a dramatic decrease in network systems and equipment costs. OpenFlow (OF) is a widely used protocol for establishing communication between the control and data planes for SDN. Reliance on a single controller might not be feasible since the controller is a single point-of-failure (SPOF) in the network. In this paper, we propose to approach the issue of fault-tolerance by using a slave controller architecture with local mechanisms of virtual controller redundancy and synchronization between the controllers. Our prototype implementation enables the network to cope with control plane crashes in the controllers without changing the OF protocol between controllers and switches.
D2 and D4 dopamine receptor polymorphisms and personality (1998)

The relationship of various dimensions of temperament, measured by the Tridimensional Personality Questionnaire (TPQ), to polymorphisms of the D2 dopamine receptor (DRD2) and D4 dopamine receptor (DRD4) genes was determined in 119 healthy Caucasian boys who had not yet begun to consume alcohol and other drugs of abuse. Total Novelty Seeking score of the TPQ was significantly higher in boys having, in common, all three minor (A1, B1, and Intron 6 1) alleles of the DRD2 compared to boys without any of these alleles. Boys with the DRD4 7 repeat (7R) allele also had a significantly higher Novelty Seeking score than those without this allele. However, the greatest difference in Novelty Seeking score was found when boys having all three minor DRD2 alleles and the DRD4 7R allele were contrasted to those without any of these alleles. Neither the DRD2 nor the DRD4 polymorphisms differentiated total Harm Avoidance score. Whereas subjects having all three minor DRD2 alleles had a significantly higher Reward Dependence 2 (Persistence) score than subjects without any of these alleles, no significant difference in this personality score was found between subjects with and without the DRD4 7R allele. In conclusion, DRD2 and DRD4 polymorphisms individually associate with Novelty Seeking behavior. However, the combined DRD2 and DRD4 polymorphisms contribute more markedly to this behavior than when these two gene polymorphisms are individually considered.
New agonist and antagonist analogues of bradykinin (1984)

Three new analogues of bradykinin (BK) have been tested for their agonistic and antagonistic actions on the rabbit jugular vein and the guinea pig ileum (B2 receptors), and six were studied on rabbit aorta strips (B1 receptors). Substitution of Gly4, Phe5, and Phe8 in BK with D-Trp gives analogues with a relative affinity lower than 1.0% as compared with BK. These analogues have no antagonistic properties on the rabbit jugular vein and on guinea pig ileum (B2 receptors). Substitution of Pro7 in des-Arg9-BK by Gly and by D-Ala give compounds that antagonise the effects of kinins on the rabbit aorta strips (B1-receptor system). These new antagonists are fairly potent with a pA2 value of 6.03 to 7.29 and seem competitive because the pA2--pA10 values approximate 0.95. These results suggest that the orientation of Phe8 is critical for the activation of B1 receptors by kinins.
Research on Embedding Capacity and Efficiency of Information Hiding Based on Digital Images (2013)

Generally speaking, being an efficient information hiding scheme, what we want to achieve is high embedding capacity of the cover image and high visual quality of the stego image, high visual quality is also called embedding efficiency. This paper mainly studies on the information hiding technology based on gray-scale digital images and especially considers the improvement of embedding capacity and embedding efficiency. For the purpose of that, two algorithms for information hiding were proposed, one is called high capacity of information hiding algorithm (HCIH for short), which achieves high embedding rate, and the other is called high quality of information hiding algorithm (HQIH for short), which realizes high embedding efficiency. The simulation experiments show that our proposed algorithms achieve better performance.
Application of vision based techniques for UAV position estimation (2016)

The objective of this project is to set up wireless communication for Unmanned Aerial Vehicles (UAVs) and to estimate the position of the UAV using vision-based techniques with an onboard camera, in a real-time, continuously interacting scenario. Here, vision based navigation is explored with different algorithms (RANSAC feature detection and normalized cross correlation with prior edge detection) and the benefits and disadvantages of each are compared. Vision based navigation uses a camera onboard the UAV to continuously transmit an aerial video of the ground to the GCS and the position of the UAV is determined using geo-referenced images such as those obtained from Google Earth.
Mechanics and Physics of Porous Solids (2010)

Foreword 1 The Strange World of Porous Solids 2 Fluid Mixtures 2.1 Chemical potential 2.2 Gibbs-Duhem Equality 2.3 Ideal Mixtures 2.4 Regular Solutions 3 The Deformable Porous Solid 3.1 Strain 3.2 Stress 3.3 Strain Work 3.4 From Solids to Porous Solids 4 The Saturated Poroelastic Solid 4.1 The Poroelastic Solid 4.2 Filling the Porous Solid 4.3 The Thermoporoelastic Solid 4.4 The Poroviscoelastic Solid 5 Fluid Transport and Deformation 5.1 Transport Laws 5.2 Coupling the Deformation and the Flow 5.3 Consolidation of a Soil Layer 6 Surface Energy and Capillarity 6.1 Physics and Mechanics of Interfaces 6.2 Capillarity in Porous Solids 6.3 Transport in Unsaturated Porous Solids 7 The Unsaturated Poroelastic Solid 7.1 Interface Stress as a Pre-Stress 7.2 Energy Balance for the Unsaturated Porous Solid 7.3 The Linear Unsaturated Poroelastic Solid 7.4 Extending Linear Unsaturated Poroelasticity 8 Uncon.ned Phase Transition 8.1 Chemical Potential and Phase Transition 8.2 Liquid-Vapor Transition 8.3 Liquid-Solid Transition 8.4 Gas bubble formation 8.5 Surface Energy and Phase Transition 9 Phase Transition in Porous Solids 9.1 In-Pore Phase Transition 9.2 Kinetics and Mechanics of Drying 9.3 Mechanics of Con.ned Crystallization 10 The Poroplastic Solid 10.1 Basic Concepts of Plasticity 10.2 From Plasticity to Poroplasticity 10.3 From material to structure 11 By Way of Conclusion
Indoor location based on WiFi (2015)

In this paper we consider indoor location based on WiFi signals under the condition that the location provider does not know the physical location of the access points. This scenario is different from most previous researches on indoor location. We proposed several algorithms for this purpose including set calibration data, calculate position and track moving rout. Results from some experiments show that our scheme is useful for many kind buildings such as Malls and office buildings.
Neutralizing Interleukin-1β (IL-1β) Induces β-Cell Survival by Maintaining PDX1 Protein Nuclear Localization (2011)

The transcription factor PDX1 plays a critical role during β-cell development and in glucose-induced insulin gene transcription in adult β-cells. Acute glucose exposure leads to translocalization of PDX1 to the nucleoplasm, whereas under conditions of oxidative stress, PDX1 shuttles from the nucleus to the cytosol. Here we show that cytosolic PDX1 expression correlated with β-cell failure in diabetes. In isolated islets from patients with type 2 diabetes and from diabetic mice, we found opposite regulation of insulin and PDX1 mRNA; insulin was decreased in diabetes, but PDX1 was increased. This suggests that elevated PDX1 mRNA levels may be insufficient to regulate insulin. In diabetic islets, PDX1 protein was localized in the cytosol, whereas in non-diabetic controls, PDX1 was in the nucleus. In contrast, overexpression of either IL-1 receptor antagonist or shuttling-deficient PDX1 restored β-cell survival and function and PDX1 nuclear localization. Our results show that nuclear localization of PDX1 is essential for a functional β-cell and provides a novel mechanism of the protective effect of IL-1 receptor antagonist on β-cell survival and function.
Multivariate statistics and neural networks in process fault detection (1995)

The objective of statistical process control (SPC) is to monitor the performance of a process over time in order to verify that it remains in a state of statistical control. Until recently, the application of SPC to manufacturing processes has been based upon the charting of a small number of variables using univariate tools. This approach is appropriate if the variables an known to exhibit independent behaviour. However, examining a limited number of preselected variables as if they were independent makes identification and interpretation of potential process malfunctions difficult, possibly misleading and dangerous. Also, malfunctions may not be identified. The multivariate techniques of principal component analysis and projection to latent structures overcome these difficulties. This is achieved by projecting the multivariate data down on to a lower dimensional space which contains all the relevant information in possibly as few as two or three latent variables, which are a linear combination of the original variables. In the fuzzy neural network fault detection approach, a preprocessing fuzzification layer is added to a conventional feedforward artificial neural net topology. The fuzzification layer converts increments in online measurements and controller outputs (deviations from steady state) into three fuzzy sets (increase/steady/decrease) with corresponding membership functions. Each output of the network represents a particular fault calibrated to the range (0,1) with a fault being indicated by an output close to 1. (8 pages)