gebhart/patrepeval huggingface dataset:
Molecular characterization of the <i>eps</i> gene cluster of <i>Pseudomonas solanacearum</i> and its transcriptional regulation at a single promoter (1995)

Production of EPS I, an unusual exopolysaccharide virulence factor of the phytopathogen Pseudomonas solanacearum, requires the 18 kb eps gene cluster. DNA sequence analysis of the first seven genes of eps (epsAPBCDEF), subcellular localization of their products in maxicells, and phoA fusion analysis showed that: (i) epsA, epsB, epsE, and epsF encode exported or membrane-associated proteins probably involved in polymerization and/or export of EPS I; (ii) epsC and epsD encode soluble enzymes probably involved in synthesis of sugar components of EPS I (N-acetylgalactosaminuronic acid and possibly N-acetyltrideoxygalactose, respectively); and (iii) epsP probably encodes a phosphatase involved in EPS I production in an unknown way. Non-polar insertional mutagenesis showed that most, if not all, of these eps genes are absolutely required for production of EPS I. Using random eps::lacZ fusions and primer extension we located a transcription start site and promoter upstream of epsA. Analysis of a plasmid with this promoter fused to lacZ showed that a 140 bp regulatory region upstream of the eps transcription start site was sufficient for normal regulation of eps transcription by the multicomponent virulence gene regulatory network of P. solanacearum. Deletion of this eps promoter from a plasmid-borne epsAPBCDE::lacZ fusion reduced its expression 10-fold, indicating that this promoter alone is responsible for regulated transcription of an eps operon composed of at least epsAPBCDE. Analysis of genomic and plasmid-borne eps::lacZ fusions suggested that most remaining eps genes are part of this same operon or, and this is less likely, comprise a second co-ordinately regulated eps operon.
Chemical Constituents of Propolis from Myanmar and Their Preferential Cytotoxicity against a Human Pancreatic Cancer Cell Line (2009)

A methanolic extract of propolis obtained in Myanmar was found to inhibit PANC-1 human pancreatic cancer cells preferentially under nutrient-deprived conditions (NDM), with a PC(50) value of 9.3 microg/mL. Bioactivity-guided fractionation of the extract led to the isolation of two new cycloartane-type triterpenes, (22Z,24E)-3-oxocycloart-22,24-dien-26-oic acid (1) and (24E)-3-oxo-27,28-dihydroxycycloart-24-en-26-oic acid (2), together with 13 cycloartanes (3-13) and four known prenylated flavanones (14-17). Among these, compound 1 exhibited the most potent preferential cytotoxicity (PC(50) 4.3 microM) in a concentration- and time-dependent manner. Furthermore, 1 induced apoptosis-like morphological changes of PANC-1 cells within 24 h of treatment.
Fault diagnosis for turbine generator based on wavelet packet PCA-SVM (2010)

As to the existing shortcomings of the traditional method for fault diagnosis of turbine generator, A new method based on wavelet packet PCA-SVM is proposed in this paper. First of all, take a wavelet packet transformation of the fault samples to extract the energy of each frequency band, and use them as the initial samples, and then make a data compression and feature extraction of the initial samples using the principal component analysis(PCA), eliminating the correlation between data and extracting the principal components which contain sufficient information of initial samples. Finally, we take the principal components as the input vectors of the support vector machines(SVM), this will reducing the dimension of the sample space and computing complexity. Simulation results shows that this method can effectively improve the diagnostic accuracy.
Leveraging Technology: The Use of a Company Intranet and Portal Technology to Improve Productivity and Quality (2004)

This session will provide a case study on how Walter P. Moore and Associates is leveraging technology to improve productivity and quality. The session will focus on two technologies, a corporate intranet and a corporate portal. Among the areas to be addressed will be the development of the technologies, the continuous improvement and leveraging of the technologies, and practical examples of the use of each technology.
Upright Radiation Therapy—A Historical Reflection and Opportunities for Future Applications (2020)

Since the early days of megavoltage Radiation Therapy (RT), the potential of delivering treatment to a sub group of patients in an upright position has been recognized. Compared to lying horizontally, treating patients in an upright position offers potential benefits in terms of patient comfort especially for patients experiencing dyspnoea and saliva accumulation when lying down. Dosimetric benefits can also be gained from changes in the volume and location of lungs and heart in an upright position, which are potentially advantageous for clinical situations including Hodgkin's disease, lung and breast malignancies. Since the 1950's, upright stabilization mechanisms have ranged from standalone chair based apparatus to couch-top attachments with increasingly customizable solutions. The introduction of Computed-Tomography (CT) based three-dimensional (3D) dosimetry in the 1980's-90's necessitated image acquisition in a horizontal position (supine or prone), significantly reducing options for alternative patient positioning and upright techniques. Despite this, upright techniques have still been utilized where clinically indicated for palliative and novel approaches often involving non-standard treatment scenarios. More recently, a small number of centers have reported on specialized equipment capable of acquiring planning data with the patient in a vertical position. The possibility of acquiring planning quality Cone Beam CT (CBCT) on linear accelerators has recently reinvigorated the potential to deliver highly accurate and targeted treatments to patients in an upright position. This paper reflects on the historical applications of upright RT and explores new possibilities for this technology in modern RT departments.
The Incidence of Molluscum contagiosum among American Indians and Alaska Natives (2009)

This study highlights the need for periodic population-based measurements to assess trends in incidence and healthcare utilization for MC in the United States. High rates of MC were found among AI/AN persons, especially among children <15 years old. The AI/AN population would benefit from greater availability of effective strategies for prevention and treatment of MCV infection.
Effects of nonpathogenic gram-negative bacterium<i>Vitreoscilla filiformis</i>lysate on atopic dermatitis: a prospective, randomized, double-blind, placebo-controlled clinical study (2008)

V. filiformis lysate significantly improved AD. This may be in part due to reduction of S. aureus, but seems to relate in most parts to a direct immunomodulatory effect on skin-associated immune responses.
Visualizing Hydrogen Sulfide in Mitochondria and Lysosome of Living Cells and in Tumors of Living Mice with Positively Charged Fluorescent Chemosensors (2016)

Two fluorescent chemosenors, Mito-HS and Lyso-HS, were rationally designed and synthesized with positive charge at physiological conditions. The positive charge showed triple functions as target moieties for subcellular mitochondria and lysosome of living cells, soluble moieties for chemosenors, as well as effective sequesters of HS(-) (H2S at physiological conditions). These two probes showed faster and more efficient fluorescence H2S detection than the similar literature-reported probe without positive charge. In addition, visualizing of hydrogen sulfide in tumors of living mice was successfully achieved for the first time using the probe Mito-HS.
An approach to decentralized computer systems (1986)

The technology for distributed computing is available. However, it is argued that decentralized systems will always require more careful design, planning, and management than their centralized counterparts. The rationale for and against decentralization is given, and a technical approach to decentralized systems is sketched. This approach contrasts with the popular concept of a distributed integrated database which transparently provides remote IO against single system image. Rather, it proposes that functions be distributed as `servers' which abstract data as high-level operations on objects and communicate with `requestors' via a standard message protocol. This requestor-server approach has the advantages of modularity and performance.
Clinical significance of endothelin in cardiovascular disease (1997)

Endothelins are ubiquitously produced 21-amino-acid peptides that were discovered as an endothelial product and may play important roles in cardiovescular physiology and pathophysiology. The main endothelin produced by the endothelium is endothelin-1. The vasoconstrictor role of endothelins may participate in blood pressure elevation and vascular hypertrophy in salt-dependent models of hypertension (deoxycorticosterone acetate-salt hypertensive rats, spontaneously hypertensive rats treated with deoxycorticosterone, acetate and salt, and Dehl salt-sensitive rats), and in stroke-prone spontaneously hypertensive rats. In humans, endothelins may play important roles in moderate to severe essential hypertension, and in the hypertension of African-Americans. Endothelins may be involved in cardiac hypertrophy, and there is increasing evidence of their participation in heart failure, in which acute endothelin antagonism in humans exerts beneficial effects. Endothelin expression is enhanced in smooth muscle cells migrating into the intima of arteries in atherosclerosis, suggesting a role in atherogenesis. Endothelin may participate as a vasoconstrictor in coronary artery disease, and as a contributor to intimal proliferation in restenosis after coronary angioplasty. In patients with myocardial infarction, cardiac production of endothelin is increased, particularly in those with cardiogenic shock. There is a potential for participation of endothelins in vasospasm accompanying stroke or subarachnoid hemorrhage: in the latter, endothelin antagonism has shown beneficial effects in experimental models. In neonatal and in primary pulmonary hypertension, endothelin expression is enhanced, and in experimental models endothelin antagonism resulted in favorable responses. Systemic sclerosis is another, peripheral, form of vascular disease in which endothelin may play a role and in which endothelin antagonism may be an interesting therapeutic alternative. The pathophysiologic role of endothelins is becoming increasingly apparent in cardiovascular disease, generating interesting potential therapeutic targets for the use of endothelin antagonists or endothelin-converting enzyme inhibitors.
A Framework for Emulating Database Operations in Cloud Data Warehouses (2020)

In recent years, increased interest in cloud-based data warehousing technologies has emerged with many enterprises moving away from on-premise data warehousing solutions. The incentives for adopting cloud data warehousing technologies are many: cost-cutting, on-demand pricing, offloading data centers, unlimited hardware resources, built-in disaster recovery, to name a few. There is inherent difference in the language surface and feature sets of on-premise and cloud data warehousing solutions. This could range from subtle syntactic and semantic differences, with potentially big impact on result correctness, to complete features that exist in one system but are missing in other systems. While there have been some efforts to help automate the migration of on-premise applications to new cloud environments, a major challenge that slows down the migration pace is the handling of features not yet supported, or partially supported, by the cloud technologies. In this paper we build on our earlier work in adaptive data virtualization and present novel techniques that allow running applications utilizing sophisticated database features within foreign query engines lacking the native support of such features. In particular, we introduce a framework to manage discrepancy of metadata across heterogeneous query engines, and various mechanisms to emulate database applications code in cloud environments without any need to rewrite or change the application code.
Network Interrupts: A Programmer's Reference to Network Apis (1994)

Network Interrupts is a comprehensive reference to network-related interrupt calls on IBM PCs and compatible machines. It includes calls from over three dozen major application interfaces and various resident utilities. In addition, this book is the only available source of information on potential conflicts between calls from different network APIs.This book includes complete coverage of the following interrupt services and topics: * MS-DOS network support * FTP and other TCP/IP software * NetBIOS * LANtastic network operating system * DESQview/X networking * Network serial I/O emulation * Novell's NetWare shell and utilities * Open Data-Link Interface * Microsoft's LAN Manager * IBM mainframe connectivity. A companion volume, PC Interrupts: A Programmer's Reference to BIOS, DOS, and Third Party Calls provides concise descriptions of all the system calls from many different sources--MS-DOS, the ROM BIOS, and various APIs (application program interfaces) such as Windows 3.x, DESQview, and Advanced Power Management. Together these books represent the most comprehensive reference to PC interrupt calls available. 0201626446B04062001
Antimicrobial resistance and prevalence of resistance genes in intestinal Bacteroidales strains (2011)

Our results indicate an increase in the resistance to several antibiotics in intestinal Bacteroides spp. and Parabacteroides distasonis and demonstrate that these microorganisms harbor antimicrobial resistance genes that may be transferred to other susceptible intestinal strains.
Biliary glycoprotein, a potential human cell adhesion molecule, is down-regulated in colorectal carcinomas. (1993)

Biliary glycoprotein (BGP) is the human homologue of a cell adhesion molecule (CAM) of the rat designated Cell-CAM. The BGP gene is a member of the carcinoembryonic antigen gene family, which belongs to the immunoglobulin superfamily. BGP is expressed in cells of epithelial and myeloid origin. In granulocytes, BGP is a main antigen of the CD66 cluster of differentiation antigens that mediate the binding to endothelial E-selectin. Since BGP is a major human CAM, the expression of BGP was studied in 21 colorectal carcinoma tissue specimens and in the respective adjacent normal mucosae. As an internal control for epithelial mRNA, the expression of cytokeratin 18 was evaluated in parallel. In addition, the expression of carcinoembryonic antigen and nonspecific crossreacting antigen, which are highly homologous to BGP, was investigated. Two BGP mRNAs of 3.9 and 1.5 kilobases were detected in the normal colonic mucosa samples. The median of the tumor-to-normal ratios of mRNA expression was 0.2 for both BGP mRNAs. In contrast, the median was 1.2 for cytokeratin, 1.0 for carcinoembryonic antigen, and 1.4 for nonspecific crossreacting antigen. Relative to cytokeratin 18 expression, the expression of BGP was reduced to < or = 0.1 in half of the tumors and to < or = 0.4 in > 80% of the tumors. These findings indicate that the loss or reduced expression of the adhesion molecule BGP is a major event in colorectal carcinogenesis.
Site-Selective RNA Splicing Nanozyme: DNAzyme and RtcB Conjugates on a Gold Nanoparticle (2017)

Modifying RNA through either splicing or editing is a fundamental biological process for creating protein diversity from the same genetic code. Developing novel chemical biology tools for RNA editing has potential to transiently edit genes and to provide a better understanding of RNA biochemistry. Current techniques used to modify RNA include the use of ribozymes, adenosine deaminase, and tRNA endonucleases. Herein, we report a nanozyme that is capable of splicing virtually any RNA stem-loop. This nanozyme is comprised of a gold nanoparticle functionalized with three enzymes: two catalytic DNA strands with ribonuclease function and an RNA ligase. The nanozyme cleaves and then ligates RNA targets, performing a splicing reaction that is akin to the function of the spliceosome. Our results show that the three-enzyme reaction can remove a 19 nt segment from a 67 nt RNA loop with up to 66% efficiency. The complete nanozyme can perform the same splice reaction at 10% efficiency. These splicing nanozymes represent a new promising approach for gene manipulation that has potential for applications in living cells.
Radial basis function neural networks: a topical state-of-the-artsurvey (2016)

Abstract Radial basis function networks (RBFNs) have gained widespread appeal amongst researchers and have shown good performance in a variety of application domains. They have potential for hybridization and demonstrate some interesting emergent behaviors. This paper aims to offer a compendious and sensible survey on RBF networks. The advantages they offer, such as fast training and global approximation capability with local responses, are attracting many researchers to use them in diversified fields. The overall algorithmic development of RBF networks by giving special focus on their learning methods, novel kernels, and fine tuning of kernel parameters have been discussed. In addition, we have considered the recent research work on optimization of multi-criterions in RBF networks and a range of indicative application areas along with some open source RBFN tools.
El Estado federal experimental (2006)

Pro-inflammatory cytokines produced in the central nervous system (CNS) have been suggested to have a role in the anorexia and cachexia of disease. In the present study, the effects of chronic exposure of the CNS to interleukin-1beta (IL-1beta) on several indicators of cachexia were studied. Rats were prepared with an intracerebroventricular (i.c.v.) cannula and an osmotic minipump that delivered vehicle or 1.56 ng/h recombinant murine IL-1beta for 4 days. Food intake and body weight were determined daily during the 4-day infusion period and plasma IL-6 and corticosterone concentrations were determined from plasma collected postinfusion. Chronic i.c.v. infusion of IL-1beta resulted in a chronic reduction in food intake. Rats infused i.c.v. with IL-1beta ate less food each day compared to vehicle controls and, at the end of the 4-day infusion period, consumed an average of 17.2 g less. Intracerebroventricular infusion of IL-1beta also caused an immediate and substantial loss of body weight that was sustained throughout the infusion period. In addition, rats infused with IL-1beta had plasma levels of IL-6 double those of vehicle controls (401 pg/ml vs. 185 pg/ml). Plasma corticosterone concentrations were similar between treatments. These results suggest that chronic exposure of the CNS to cytokines such as IL-1beta may be sufficient to induce anorexia and cachexia.
The Impact of Anatomical Radical Retropubic Prostatectomy on Cancer Control: The 30-Year Anniversary (2012)

Excellent prostate cancer specific survival was demonstrated up to 30 years after surgery. Clinical risk categories and pathological tumor features were significant predictors of long-term disease specific outcomes, supporting their ongoing use in risk stratification and management decisions. Anatomical radical retropubic prostatectomy continues to represent the gold standard in the surgical management of clinically localized prostate cancer to which alternate treatment options should be compared.
Elemental electrical switch enabling phase segregation–free operation (2021)

Nonvolatile phase-change memory has been successfully commercialized, but further density scaling below 10 nanometers requires compositionally and structurally homogeneous materials for both the memory cell and the associated vertically stacked two-terminal access switch. The selector switches are mostly amorphous-chalcogenide Ovonic threshold switches (OTSs), operating with a nonlinear current response above a threshold voltage in the amorphous state. However, they currently suffer from the chemical complexity introduced by the quaternary or even more diverse chalcogenide compositions used. We present a single-element tellurium (Te) volatile switch with a large (≥11 megaamperes per square centimeter) drive current density, ~10<sup>3</sup> ON/OFF current ratio, and faster than 20 nanosecond switching speed. The low OFF current arises from the existence of a ~0.95–electron volt Schottky barrier at the Te–electrode interface, whereas a transient, voltage pulse–induced crystal-liquid melting transition of the pure Te leads to a high ON current. Our discovery of a single-element electrical switch may help realize denser memory chips.
Simultaneous Prevention of Rotational and Translational Slip for a Humanoid Robot (2018)

Slip often occurs in humanoid robot walking, especially when the robot walks on a low friction floor or walks fast. Unexpected slip may cause the robot to fall and then sustain damage. In real environments, rotational and translational slip phenomena can happen during biped walking. Previous studies have mainly focused on solving these problems independently. In this paper, we propose strategies for simultaneous rotational and translational slip prevention based on a three mass model, which takes into account the effect of the swing leg. The rotational slip is prevented through a bionic walking pattern generator which mimics the yaw moment compensation mechanism of a human. The translational slip is eliminated through a novel reaction force ratio reduction control with the compensation of CoM (center of mass) acceleration. The effectiveness of the presented strategies is validated by simulations and experiments with an actual humanoid robot.
Molecular and Genetic Analysis of the Gene Encoding the <i>Saccharomyces cerevisiae</i> Strand Exchange Protein Sep1 (1991)

Vegetatively grown Saccharomyces cerevisiae cells contain an activity that promotes a number of homologous pairing reactions. A major portion of this activity is due to strand exchange protein 1 (Sep1), which was originally purified as a 132,000-Mr species (R. Kolodner, D. H. Evans, and P. T. Morrison, Proc. Natl. Acad. Sci. USA 84:5560-5564, 1987). The gene encoding Sep1 was cloned, and analysis of the cloned gene revealed a 4,587-bp open reading frame capable of encoding a 175,000-Mr protein. The protein encoded by this open reading frame was overproduced and purified and had a relative molecular weight of approximately 160,000. The 160,000-Mr protein was at least as active in promoting homologous pairing as the original 132,000-Mr species, which has been shown to be a fragment of the intact 160,000-Mr Sep1 protein. The SEP1 gene mapped to chromosome VII within 20 kbp of RAD54. Three Tn10LUK insertion mutations in the SEP1 gene were characterized. sep1 mutants grew more slowly than wild-type cells, showed a two- to fivefold decrease in the rate of spontaneous mitotic recombination between his4 heteroalleles, and were delayed in their ability to return to growth after UV or gamma irradiation. Sporulation of sep1/sep1 diploids was defective, as indicated by both a 10- to 40-fold reduction in spore formation and reduced spore viability of approximately 50%. The majority of sep1/sep1 diploid cells arrested in meiosis after commitment to recombination but prior to the meiosis I cell division. Return-to-growth experiments showed that sep1/sep1 his4X/his4B diploids exhibited a five- to sixfold greater meiotic induction of His+ recombinants than did isogenic SEP1/SEP1 strains. sep1/sep1 mutants also showed an increased frequency of exchange between HIS4, LEU2, and MAT and a lack of positive interference between these markers compared with wild-type controls. The interaction between sep1, rad50, and spo13 mutations suggested that SEP1 acts in meiosis in a pathway that is parallel to the RAD50 pathway.