gebhart/patrepeval huggingface dataset:
The Encapsulation of Cell-free Transcription and Translation Machinery in Vesicles for the Construction of Cellular Mimics (2013)

As interest shifts from individual molecules to systems of molecules, an increasing number of laboratories have sought to build from the bottom up cellular mimics that better represent the complexity of cellular life. To date there are a number of paths that could be taken to build compartmentalized cellular mimics, including the exploitation of water-in-oil emulsions, microfluidic devices, and vesicles. Each of the available options has specific advantages and disadvantages. For example, water-in-oil emulsions give high encapsulation efficiency but do not mimic well the permeability barrier of living cells. The primary advantage of the methods described herein is that they are all easy and cheap to implement. Transcription-translation machinery is encapsulated inside of phospholipid vesicles through a process that exploits common instrumentation, such as a centrifugal evaporator and an extruder. Reactions are monitored by fluorescence spectroscopy. The protocols can be adapted for recombinant protein expression, the construction of cellular mimics, the exploration of the minimum requirements for cellular life, or the assembly of genetic circuitry.
Comparison of measured and theoretical statistical parameters of complex cavities (2002)

Reverberation chambers have grown in popularity for radiated immunity testing. Unlike most other types of immunity tests, a reverberation chamber provides a randomized electromagnetic environment and is a statistical test. In a properly operated reverberation chamber the electromagnetic environment is isotropic and randomly polarized. These characteristics provide a robust, all aspect angle test. A number of the statistical characteristics of the electromagnetic environment in a reverberation chamber can be predicted from theory. This paper compares theoretical and measured values of selected statistical parameters from seven reverberation chambers in the Naval Surface Warfare Center, Dahlgren Division Database. Also presented are the same parameters from measurements in five, low quality factor, aircraft cavities.
Updated Design Methods for HPHT Equipment (2005)

Abstract Drilling industry technology is advancing rapidly. Drillers are encountering downhole pressures over 20,000 psi and temperatures over 450°F. These high pressure high temperature (HPHT) conditions require drilling and completion equipment that is beyond the scope of today's API specifications. API specifications 6A1, 16A2, 16C3, and 17D4 address the design and design verification methods for drilling equipment. These specifications currently reference the ASME Boiler and Pressure Vessel Code, Section VIII, Division 25 (ASME VIII-2) as one of the primary design verification methodologies. API specifications first referenced ASME VIII-2 as a design verification methodology nearly 20 years ago, because it was the best available at that time. This is called the "ASME Method". The ASME Section VIII, Division 36 (ASME VIII-3) was developed to give the requirements for the construction of high pressure vessels. ASME VIII-3 was first issued in 1997 and is intended to be used in place of ASME VIII-2 for high pressure vessels, generally in excess of 10,000 psi. The design of drilling and completion equipment should be done using first principles. The authors propose that the designs for 15,000 psi or higher service should be verified using the rules from ASME VIII-3. A significant difference between the two design verification methods is that the user specifies the loading criteria for the performance-based ASME VIII-3. ASME VIII-3 requires cyclic load (fatigue or fracture mechanics) analysis and has limitations in materials, fabrication and inspections, and testing requirements that specifically apply to thick-walled pressure vessels. This paper discusses how the performance-based code methods of ASME VIII-3 can be integrated into API specifications for HPHT drilling and completion equipment.
Iterative Posterior-Based Keyword Spotting Without Filler Models (1999)

This paper addresses the problem of detecting keywords in unconstrained speech without explicit modeling of nonkeyword segments. The proposed algorithm is based on recent developments in confidence measures using local posterior probabilities, and searches for the segment maximizing the average observation posterior 1 along the most likely path in the hypothesized keyword model. 2 As known, this approach (sometimes referred to as sliding model method) requires a relaxation of the begin/endpoints of the Viterbi matching, as well as a time normalization of the resulting score, making dynamic programming sub-optimal or more complex (more computation and/or more memory). We present here an alternative (quite simple and efficient) solution to this problem, using an iterative form of Viterbi decoding algorithm, but which does not require scoring for all possible begin/endpoints. Convergence proof of this algorithm is available [8]. Results obtained with this method on 100 keywords chosen at random from the BREF database [5] are reported. 1.
Differential Helmholtz resonator as an optoacoustic detector (1979)

Two identical optoacoustic cells are connected to give a Helmholtz resonator. The phase and magnitude of the signals from both cells are investigated. It is shown that an enhancement of the SNR is obtained using the phase properties of the signals.
Learning 3D-aware Egocentric Spatial-Temporal Interaction via Graph Convolutional Networks (2019)

To enable intelligent automated driving systems, a promising strategy is to understand how human drives and interacts with road users in complicated driving situations. In this paper, we propose a 3D-aware egocentric spatial-temporal interaction framework for automated driving applications. Graph convolution networks (GCN) is devised for interaction modeling. We introduce three novel concepts into GCN. First, we decompose egocentric interactions into ego-thing and ego-stuff interaction, modeled by two GCNs. In both GCNs, ego nodes are introduced to encode the interaction between thing objects (e.g., car and pedestrian), and interaction between stuff objects (e.g., lane marking and traffic light). Second, objects' 3D locations are explicitly incorporated into GCN to better model egocentric interactions. Third, to implement ego-stuff interaction in GCN, we propose a MaskAlign operation to extract features for irregular objects. We validate the proposed framework on tactical driver behavior recognition. Extensive experiments are conducted using Honda Research Institute Driving Dataset, the largest dataset with diverse tactical driver behavior annotations. Our framework demonstrates substantial performance boost over baselines on the two experimental settings by 3.9% and 6.0%, respectively. Furthermore, we visualize the learned affinity matrices, which encode ego-thing and ego-stuff interactions, to showcase the proposed framework can capture interactions effectively.
Transcript levels of <i>AtMRPs</i> after cadmium treatment: induction of <i>AtMRP3</i> (2003)

ABSTRACT In yeasts, the ABC‐type transporters are involved in vacuolar sequestration of cadmium. In plants, transport experiments with isolated vacuoles indicate that this is also true. In order to know more about the response of AtMRPs, a subclass of Arabidopsis ABC transporters, to cadmium, their expression pattern was analysed using the microchip technology and semi‐quantitative reverse transcriptase‐polymerase chain reaction. From 15 putative sequences coding for AtMRPs, transcript levels were detected for 14. All were expressed in the roots as well as in the shoots, although at a different level. In 4‐week‐old Arabidopsis , transcript levels of four AtMRPs were up‐regulated after cadmium treatment. In all cases up‐regulation was exclusively observed in the roots. The increase of transcript levels was most pronounced for AtMRP3 . A more detailed analysis revealed that induction of AtMRP3 could also be observed in the shoot when leaves were cut and cadmium allowed to be taken up in the shoot. In young plantlets, a far higher portion of Cd 2+ was translocated in the aerial part compared with adult plants. Consequently, AtMRP3 transcript levels increased in both root and shoot of young plants. This suggests that 7‐day‐old seedlings do not exhibit such a strict root–shoot barrier as 4‐week‐old plants. Expression analysis with mutant plants for glutathione and phytochelatin synthesis as well as with compounds producing oxidative stress indicate that induction of AtMRP3 is likely due to the heavy metal itself.
Acetylcholine receptor antibody in myasthenia gravis: predominance of IgG subclasses 1 and 3. (1987)

The concentrations of IgG subclass antibodies (Ab) to acetylcholine receptor (AchR) were quantified in 36 patients with myasthenia gravis (MG) treated with pyridostigmine only, and in eight patients who underwent thymectomy, using an IgG subclass-specific immunoprecipitation assay. IgG1, IgG2, IgG3, and IgG4 subclass Ab to AchR were present in 100%, 33%, 64% and 39% of the pyridostigmine-treated patients, respectively. The concentration of IgG1 Ab increased significantly with disease severity as graded by the Osserman-Genkins classification (rs = 0.37, P less than 0.05). IgG1 and IgG3 subclass protein concentrations were significantly higher (P less than 0.0003) in the 36 pyridostigmine-treated MG patients than in 44 age- and sex-matched healthy subjects. Thymectomy induced an appreciable reduction in anti-AchR IgG1 concentration in two patients, whereas six patients showed no changes in Ab to AchR. The results support the hypothesis that binding of anti-AchR IgG1 and IgG3 on AchR in the neuromuscular junction followed by complement-mediated cell lysis or phagocytosis, may play a role in the pathogenesis of MG.
Controlled growth of ZnO nanoflowers on nanowall and nanorod networks via a hydrothermal method (2014)

This study developed a hydrothermal method for the growth of three types of zinc oxide (ZnO) nanostructures: nanorods, nanowalls and nanoflowers. The structures are produced at high densities with a high degree of uniformity on Al-coated SiO2 substrates without the need for surfactant. Unlike the random distribution of ZnO nanoflowers reported in previous studies, the proposed method makes it possible to control the distribution of these structures along the grooves created by altering the growth rate of ZnO nanorods and nanowalls. The number of ZnO nanoflowers created in this manner depends on the concentration of solution (HMT: hexamethylenetetramine) and reaction time. Measurements of cathodoluminescence (CL), X-ray diffractometry (XRD), and SEM-EDS confirm that the resulting structures are pure ZnO with good crystallinity. We also investigated the optical properties of these ZnO nanostructures and propose a possible growth mechanism.
Eigenspannungen, Definition und Entstehungsursachen (1979)

Abstract Die vor einigen Jahren vorgestellte Eigenspannungsdefinition, die sich auf den realen Aufbau technischer Werkstoffe und nicht auf ein Begriffssystem der Meßverfahren bezieht, hat sich bewährt, da hiermit eine wesentliche Vereinheitlichung der Begriffe und eine fachgerechte Deutung von Eigenspannungs‐Entstehungsursachen erreicht wurde. Eigenspannungen I., II. und III. Art unterscheiden sich im wesentlichen durch die Größenordnung der Eigenspannungsquellen. Eigenspannungen treten meist in überlagerter Form auf und basieren häufig auf einer Überlagerung mehrerer z. T. gegenläufiger Entstehungsursachen. Bei bekanntem Hauptachsensystem und bekannter Verteilung der mehrachsialen Eigenspannungen über den Gesamtquerschnitt von Probestäben und Bauteilen kann durch geeignete Mehrachsigkeits‐Kenngrößen die Überlagerung von Last‐ und Eigenspannungen I. Art eindeutig formuliert werden.
Molecular Insights into Carbon Dioxide Sorption in Hydrazone-Based Covalent Organic Frameworks with Tertiary Amine Moieties (2019)

Tailorable sorption properties at the molecular level are key for efficient carbon capture and storage and a hallmark of covalent organic frameworks (COFs). Although amine functional groups are known to facilitate CO<sub>2</sub> uptake, atomistic insights into CO<sub>2</sub> sorption by COFs modified with amine-bearing functional groups are scarce. Herein, we present a detailed study of the interactions of carbon dioxide and water with two isostructural hydrazone-linked COFs with different polarities based on the 2,5-diethoxyterephthalohydrazide linker. Varying amounts of tertiary amines were introduced in the COF backbones by means of a copolymerization approach using 2,5-bis(2-(dimethylamino)ethoxy)terephthalohydrazide in different amounts ranging from 25 to 100% substitution of the original DETH linker. The interactions of the frameworks with CO<sub>2</sub> and H<sub>2</sub>O were comprehensively studied by means of sorption analysis, solid-state NMR spectroscopy, and quantum-chemical calculations. We show that the addition of the tertiary amine linker increases the overall CO<sub>2</sub> sorption capacity normalized by the surface area and of the heat of adsorption, whereas surface areas and pore size diameters decrease. The formation of ammonium bicarbonate species in the COF pores is shown to occur, revealing the contributing role of water for CO<sub>2</sub> uptake by amine-modified porous frameworks.
GaN-HEMT-based three level T-type NPC inverter using reverse-conducting mode in rectifying (2017)

The paper presents a design method of a gallium-nitride high-electron-mobility-transistor (GaN-HEMT) based three level T-type neutral-point-clamped (NPC) inverter using a reverse-conducting mode of the GaN-HEMT. The GaN-HEMT provides high-frequency switching speed and the T-type inverter supports such switching by decreasing conduction loss and heat dissipation. The GaN-HEMT has two operation mode, an enhancement mode and the reverse-conducting mode. In the enhancement mode, resistance on the GaN-HEMT is controlled by gate-source voltage. The reverse-conducting mode appears when gate-source voltage is less than zero. This characteristic provides advantage on design of the T-type NPC inverter. Then, the paper shows that a normally-off inverter is easily attained by using the reverse-conducting mode. Verification of the designed-circuit is conducted by some validation.
Characterization of high molecular weight compounds in urban atmospheric particles (2005)

Abstract. The chemical nature of a large mass fraction of ambient organic aerosol particles is not known. High molecular weight compounds (often named humic-like substances) have recently been detected by several authors and these compounds seem to account for a significant fraction of the total organic aerosol mass. Due to the unknown chemical structure of these compounds quantification as well as a determination of their molecular weight is difficult. In this paper we investigate water soluble humic-like substances in ambient urban aerosol using size exclusion chromatography-UV spectroscopy and laser desorption/ionization mass spectrometry (LDI-MS). LDI-MS was used for the first time to investigate HULIS from atmospheric aerosols. A careful evaluation of the two method shows that both methods complement each other and that both are needed to learn more about the molecular weight distribution and the concentration of humic-like substances. An upper molecular weight limit of humic-like substances of about 700 Da and a concentration of 0.3-1.6 µg/m3 air can be estimated, corresponding to 9-30% of the total organic carbon for an urban background site.
Identification and characterization of Src SH3 ligands from phage-displayed random peptide libraries. (2021)

We have used the Src homology 3 (SH3) domain to screen two phage-displayed random peptide libraries, each containing 2 x 10(8) unique members, and have identified a series of high affinity peptide ligands. The peptides possess similar proline-rich regions, which yield a consensus Src SH3-binding motif of RPLPPLP. We have confirmed this motif by screening a phage-displayed peptide library biased for SH3 ligands and identifying the same consensus sequence. Binding studies using synthetic peptides suggest that the RPLPPLP motif is important for SH3 binding and confers specificity for the Src SH3 domain, and that residues which flank the motif may also contribute to binding. Peptides that contain the RPLPPLP motif compete Src, but not Abl or phospholipase C gamma, SH3 interactions with SH3-binding proteins from cell lysates (IC50 = 1-5 microM). Furthermore, RPLPPLP-related peptides are able to accelerate progesterone-induced maturation of Xenopus laevis oocytes. A similar acceleration has been observed in oocytes treated with activated, but not normal, Xenopus Src, suggesting the possibility that the peptides are able to antagonize the negative regulation of Src activity by Src SH3 in vivo.
Depletion of U14 small nuclear RNA (snR128) disrupts production of 18S rRNA in Saccharomyces cerevisiae. (1990)

Repression of an essential nucleolar small nuclear RNA (snRNA) gene of Saccharomyces cerevisiae was shown to result in impaired production of 18S rRNA. The effect, observed for an snRNA species of 128 nucleotides (snR128), was evident within one generation after the onset of SNR128 gene repression and correlated well with depletion of the snRNA. The steady-state mass ratio of 18S RNA to 25S RNA decreased eightfold over the course of the analysis. Results from pulse-chase assays revealed the basis of the imbalance to be underaccumulation of 18S RNA and its 20S precursor. This effect appears to result from impairment of processing of the 35S rRNA transcript at sites that define the 20S species coupled with rapid turnover of unstable intermediates. Possible bases for the effects observed are discussed. A common U14 designation is proposed for the structurally related yeast snRNA and 4.5S hybRNAs from amphibians and mammals.
The Structure and Performance of Efficient Interpreters. (2003)

Due to the limited width of the SPC muscle, a margin in excess of 2 mm may not be attainable in a transoral radical tonsillectomy. Margin status may be ideally determined by the integrity of the SPC muscle in future oncologic studies, rather than an adequate distance measurement.
Inflationary oscillometric blood pressure monitoring: validation of the OMRON-MIT (2002)

Inflationary oscillometry is a valid technique for the measurement of blood pressure. The OMRON-MIT, which uses this technique, is accurate and can be recommended for clinical use in the adult population.
ChemInform Abstract: A Novel Route to Multiwalled Carbon Nanotubes and Carbon Nanorods at Low Temperature. (2002)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Improved delay time estimation of RC ladder networks (2000)

An improved delay time bound estimation is presented for a n-cell RC ladder network with capacitive load. A RC lumped model of the networks is use at the design stage, as it give a faster prediction.
Residues in the longitudinal, hydrophobic strip-of-helix relate to terminations and crossings of alpha-helices. (1992)

An alpha-helix terminates when the virtual extension of its most hydrophobic, longitudinal strip containing Leu, Ile, Val, Phe, and Met lacks those residues. In each of 247 helices a template was fitted to maximize the mean hydrophobicity of positions forming a longitudinal strip-of-helix. The template was then extended into sequences beyond the ends of the helices. Leu, Ile, Val, Phe, and Met occurred in positions in the longitudinal strip-of-helix at an increased frequency (p less than 0.001), but in the first and second positions beyond either end of each true helix, they occurred at the same frequency as for their empirical distribution over all the proteins. Excesses of Asp and Glu were found in the N-terminal loop, and of Arg, His, and Lys in specific positions about the C terminus of helices. The longitudinal hydrophobic strip, the smallest amino acid in that strip, and charged amino acids in that strip, related to rotational and longitudinal orientation of alpha-helices in 15 proteins. Adjacent helices generally crossed through their longitudinal hydrophobic strips. They usually crossed through the smallest residue in the strip. Charged residues, when they occurred in the strips, were excluded from the crossing regions.
Preparation of vertically aligned carbon nanotube/polyaniline composite membranes and the flash welding effect on their supercapacitor properties (2016)

A vertically aligned SWNT/PANi membrane was fabricated by electric-filtration coupling and subsequent flash-welding method, exhibiting higher mechanical and supercapacitance properties.
Configuring and managing a large-scale monitoring network solving real world challenges for ultra low powered and long-range wireless mesh networks (2005)

In creating wireless networking solutions suitable for deployment in harsh, unpredictable, and widespread environments, we were confronted with a series of problems as-yet unsolved by commercially available technologies. The purpose of this article is to describe how we addressed mission-critical customer requirements by developing a wireless technology explicitly for devices in Ultra Low Power (ULP) and Long-Range wireless mesh networks. The key end-points in our target implementation are battery-operated devices located in hard-to-reach places, but which are nonetheless expected to offer a lifespan of several years without human intervention.We provide an overview of the technical requirements for building ULP networks, with a focus on configuration and management (including patent pending self-configuration and dynamic-routing features).This is followed by a case study of an existing 25,000 node wireless network deployed for an automatic meter reading (AMR) solution, and examples of provisioning individual nodes in complex real-world networks. We also describe how transmitting information about existing network hierarchy to new nodes not only preserves overall battery life in other network nodes, but also simplifies installation efforts significantly. The technology described here is particularly applicable to metering, telemetry, remote monitoring, and large-scale data collection solutions, while straightforwardly suited for personal and property security, medical surveillance, access control, lighting systems, as well as numerous industrial sectors.With a strong background in utility metering systems, Coronis Systems provides ready-to-use wireless solutions for manufacturers, VARs, and integrators in the automatic remote metering and wireless sensor network industries.