gebhart/patrepeval huggingface dataset:
CrmA Gene Can Protect Hepatocyte Apoptosis Induced by Anti-Fas Antibody in Mice (1999)

431 Cell mediated cytotoxicity is believed to induce allograft apoptosis via Fas/Fas-ligand and perforin/granzyme pathways. The cytokine response modifier A (CrmA), a cowpox virus gene whose gene product inhibits caspases-induced apoptosis, is considered to block the above-mentioned pathways. The purpose of the present study is to investigate that the CrmA is an effective candidate gene to inhibit apoptosis, using an anti-Fas antibody to induce lethal hepatic apoptosis as a model of graft rejection. A recombinant adenovirus vector expressing CrmA (AdexLNL-CrmA) was successfully generated using a Cre-loxP recombination system. The gene expression level was detected by the immune staining resulting in approximately 60% positive cells in liver sections obtained from the AdexLNL-CrmA and AdexCAG-NL-Cre transferred livers. Administration of agonistic anti-Fas antibody to mice rapidly led to massive liver apoptosis. By contrast, hepatic apoptosis was dramatically reduced in the mice which expressed CrmA gene. The survival of the mice increased from 50 to 100% with the increase of the administration dose from 106 pfu to 108 pfu of AdexCAG-NL-Cre, indicated that adjustment of levels of the CrmA transgene expression could be achievable by the protect effect. These results showed that CrmA gene is an effective gene to inhibit apoptosis and implicate a potential therapeutic usage of CrmA for the protection of liver damage due to allograft rejection.
Development of Stem Cell Therapies for Retinal Degeneration (2019)

Degenerative retinal disease is the major cause of sight loss in the developed world and currently there is a lack of effective treatments. As the loss of vision is directly the result of the loss of retinal cells, effective cell replacement through stem-cell-based therapies may have the potential to treat a great number of retinal diseases whatever their underlying etiology. The eye is an ideal organ to develop cell therapies as it is immune privileged, and modern surgical techniques enable precise delivery of cells to the retina. Furthermore, a range of noninvasive diagnostic tests and high-resolution imaging techniques facilitate the evaluation of any therapeutic intervention. In this review, we evaluate the progress to date of current cell therapy strategies for retinal repair, focusing on transplantation of pluripotent stem-cell-derived retinal pigment epithelium (RPE) and photoreceptor cells.
Microfabricated chip-scale rubidium plasma light source for miniature atomic clocks (2012)

We present the microfabrication and characterization of a low-power, chip-scale Rb plasma light source, designed for optical pumping in miniature atomic clocks. A dielectric barrier discharge (DBD) configuration is used to ignite a Rb plasma in a micro-fabricated Rb vapor cell on which external indium electrodes were deposited. The device is electrically driven at frequencies between 1 and 36 MHz, and emits 140 μW of stable optical power while coupling less than 6 mW of electrical power to the discharge cell. Optical powers of up to 15 and 9 μW are emitted on the Rb D2 and D1 lines, respectively. Continuous operation of the light source for several weeks has been demonstrated, showing its capacity to maintain stable optical excitation of Rb atoms in chip-scale double-resonance atomic clocks.
Experimental Researches on Resistance Heat Aided Friction Stir Welding of Mg Alloy (2013)

Resistance heat aided friction stir welding (RFSW) was utilized in this experiment with the combined effects of friction heating and resistance heating by adding the direct electric current (0-160 A) to the AZ31B magnesium alloy butt joint. The welded joints were described by optical microscope observations. Analysis of the grain boundary character distribution by optical microscope showed that the weld zone defects decreased, and smoother and more compact joint appearance was accessed by combining the direct electric current with the conventional FSW. In addition, raising the value of electric current promoted the joint quality and refined the grain size in the weld nugget zone (WNZ) and thermo mechanically affected zone (TMAZ) dramatically. However, since there are more grain growth and secondary recrystallization in HAZ of RFSW joints, the average grain size is larger than that of conventional FSW joints. The travel speed of 30 mm/min and the current value of 160A were optimum options within the parameters investigated in this work.
Electric Dipole Moments of Water Clusters from a Beam Deflection Measurement (2006)

The response of $({\mathrm{H}}_{2}\mathrm{O}{)}_{n=3--18}$ clusters to an electric field is studied by beam deflection. All clusters deflect uniformly, behaving as polarizable particles. The effective polarizabilities exceed the electronic component and increase as the clusters are cooled, revealing a large permanent dipole contribution. The results resolve a discrepancy concerning the polarity of water clusters and show that all species access conformations with moments exceeding 1 D. The data show no evidence for a freezing transition down to $\ensuremath{\sim}120\text{ }\text{ }\mathrm{K}$, but suggest a shift in the conformer arrangement at $n=8--9$.
The IL-15-Based ALT-803 Complex Enhances FcγRIIIa-Triggered NK Cell Responses and <i>In Vivo</i> Clearance of B Cell Lymphomas (2015)

ALT-803 represents a novel immunostimulatory drug that enhances NK-cell antilymphoma responses in vitro and in vivo, thereby supporting the clinical investigation of ALT-803 plus anti-CD20 mAbs in patients with indolent B-cell lymphoma.
Nanocoating hybrid polymer films on large quantities of cohesive nanoparticles by molecular layer deposition (2009)

Abstract The conformal coating of ultra‐thin aluminum alkoxide (alucone) polymer films on primary silica and titania nanoparticles using molecular layer deposition (MLD) in a fluidized bed reactor from 100 to 160 °C is described. In situ mass spectrometry revealed that the growth of alucone MLD films was self‐limiting as a function of the individual trimethylaluminum and ethylene glycol exposures. The composition and highly conformal alucone films throughout the surface of both silica and titania nanoparticles were confirmed. The highest growth rate was observed at the lowest sample temperature. Primary nanoparticles were coated individually despite their strong tendency to aggregate during fluidization. Based on the results of chemical and thermogravimetric analysis, the value of x in the formula of Al(OCH 2 CH 2 O) x was estimated to be 1.9. The calculated film density slightly increased from 2.0 ± 0.1 to 2.2 ± 0.1 g/cm 3 with the increasing of temperature from 100 to 160 °C. © 2009 American Institute of Chemical Engineers AIChE J, 2009
Silica-immobilized 2-[(2-(triethoxysily)ethyl)thio]aniline as a selective sorbent for the separation and preconcentration of palladium (1988)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSilica-immobilized 2-[(2-(triethoxysily)ethyl)thio]aniline as a selective sorbent for the separation and preconcentration of palladiumTarimala. Seshadri and Hans Juergen. HauptCite this: Anal. Chem. 1988, 60, 1, 47–52Publication Date (Print):January 1, 1988Publication History Published online1 May 2002Published inissue 1 January 1988https://pubs.acs.org/doi/10.1021/ac00152a013https://doi.org/10.1021/ac00152a013research-articleACS PublicationsRequest reuse permissionsArticle Views175Altmetric-Citations42LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
SN2‐Palmitate Reduces Fatty Acid Excretion in Chinese Formula‐fed Infants (2015)

Similar to breast milk, the SN2-palmitate infant formula primarily reduced calcium-saponified fat excretion. The results of this study further emphasize the nutritional importance of SN2-palmitate structured fat for infants.
Automated Verification of Access Control Policies (2004)

Managing access control policies in modern computer systems can be challenging and error-prone, especially when multiple access policies are combined to form new policies, possibly introducing unintended consequences. In this paper we present a framework for automated verification of access control policies. We introduce a formal model for systematically specifying access to resources. We show that the access control policies in the XACML access control language can be translated to a simple form which partitions the input domain to four classes: permit, deny, error, and notapplicable. We present several ordering relations for access control policies which can be used to specify the properties of the policies and the relationships among them. We then show how to automatically check these ordering relations using an existing automated analysis tool. In particular, we translate XACML policies to the Alloy language and check their properties using the Alloy Analyzer. Our experimental results demonstrate that automated verification of XACML policies is feasible.
Silicon Oxycarbide Films Produced by Remote Microwave Hydrogen Plasma CVD using a Tetramethyldisiloxane Precursor: Growth Kinetics, Structure, Surface Morphology, and Properties (2015)

Amorphous hydrogenated silicon oxycarbide (a‐SiCO:H) thin films are produced by remote microwave hydrogen plasma CVD using 1,1,3,3‐tetramethyldisiloxane precursor. The effect of substrate temperature ( T S ) on the chemical structure and some properties of resulting a‐SiCO:H films is reported. The examination performed by infrared spectroscopy revealed that the increase in T S involves the elimination of organic moieties from the film and its transformation from polymer‐like to ceramic‐like high‐crosslink‐density material. Due to their small surface roughness, high density, and good optical transparency, the a‐SiCO:H films seem to be useful coatings for optical and electronic devices.
Localization of natriuretic peptide-activated guanylate cyclase mRNAs in the rat brain (1996)

Physiological actions of atrial natriuretic peptide (ANP) and C-type natriuretic peptide (CNP) are elaborated by membrane-bound natriuretic peptide receptors (NPRs). These receptors possess intracellular guanylate cyclase domains that mobilize cyclic guanosine monophosphate upon binding of peptide. Two distinct NPR subtypes have been described in brain: the NPR-A selectively binds ANP, whereas NPR-B exhibits high affinity for CNP. To define further the potential domains of ANP and CNP action in brain, the present study used in situ hybridization histochemistry to map NPR-A and NPR-B mRNA-expressing cell populations. Significant levels of neuronal NPR-A mRNA expression were observed only in the mitral cell layer of the olfactory bulb, medial habenula, subfornical organ, and area postrema. Expression of NPR-A mRNA was observed in forebrain white matter tracts, suggesting synthesis in glial cells. In contrast, NPR-B mRNA was widely expressed throughout the neuraxis. In the telencephalon, signal was abundant throughout limbic cortex and neocortex, olfactory bulb, hippocampus, and amygdala. Intense NPR-B mRNA hybridization was observed in preoptic-hypothalamic neuroendocrine circuits and in motor nuclei of cranial nerves. Intermediate expression of NPR-B mRNA was observed in brainstem nuclei controlling autonomic function. Labeling for NPR-B but not NPR-A mRNA was observed in pituicytes in the neural lobe of the pituitary and in scattered cells of the anterior pituitary. These results suggest that CNP is the primary biologically active natriuretic peptide in brain. In contrast with NPR-B, NPR-A appears to be expressed largely in restricted cell populations containing high levels of ANP and in circumventricular organs. These data implicate the NPR-A in autoregulation of ANP neurons and central registration of cardiac ANP release.
Structure-function-guided exploration of the antimicrobial peptide polybia-CP identifies activity determinants and generates synthetic therapeutic candidates (2018)

Antimicrobial peptides (AMPs) constitute promising alternatives to classical antibiotics for the treatment of drug-resistant infections, which are a rapidly emerging global health challenge. However, our understanding of the structure-function relationships of AMPs is limited, and we are just beginning to rationally engineer peptides in order to develop them as therapeutics. Here, we leverage a physicochemical-guided peptide design strategy to identify specific functional hotspots in the wasp-derived AMP polybia-CP and turn this toxic peptide into a viable antimicrobial. Helical fraction, hydrophobicity, and hydrophobic moment are identified as key structural and physicochemical determinants of antimicrobial activity, utilized in combination with rational engineering to generate synthetic AMPs with therapeutic activity in a mouse model. We demonstrate that, by tuning these physicochemical parameters, it is possible to design nontoxic synthetic peptides with enhanced sub-micromolar antimicrobial potency in vitro and anti-infective activity in vivo. We present a physicochemical-guided rational design strategy to generate peptide antibiotics.
<i>AML1-ETO</i> and <i>C-KIT</i> mutation/overexpression in t(8;21) leukemia: Implication in stepwise leukemogenesis and response to Gleevec (2005)

To explore the genetic abnormalities that cooperate with AML1-ETO (AE) fusion gene to cause acute myeloid leukemia (AML) with t(8;21), we screened a number of candidate genes and identified 11 types of mutations in C-KIT gene (mC-KIT), including 6 previously undescribed ones among 26 of 54 (48.1%) cases with t(8;21). To address a possible chronological order between AE and mC-KIT, we showed that, among patients with AE and mC-KIT, most leukemic cells at disease presentation harbored both genetic alteration, whereas in three such cases investigated during complete remission, only AE, but not mC-KIT, could be detected by allele-specific PCR. Therefore, mC-KIT should be a subsequent event on the basis of t(8;21). Furthermore, induced expression of AE in U937-A/E cells significantly up-regulated mRNA and protein levels of C-KIT. This may lead to an alternative way of C-KIT activation and may explain the significantly higher C-KIT expression in 81.3% of patients with t(8;21) than in patients with other leukemias. These data strongly suggest that t(8;21) AML follows a stepwise model in leukemogenesis, i.e., AE represents the first, fundamental genetic hit to initiate the disease, whereas activation of the C-KIT pathway may be a second but also crucial hit for the development of a full-blown leukemia. Additionally, Gleevec suppressed the C-KIT activity and induced proliferation inhibition and apoptosis in cells bearing C-KIT N822K mutation or overexpression, but not in cells with D816 mC-KIT. Gleevec also exerted a synergic effect in apoptosis induction with cytarabine, thus providing a potential therapeutic for t(8;21) leukemia.
Carbohydrate fluctuations, gas vacuolation, and vertical migration of scum‐forming cyanobacteria in fishponds1 (1985)

Natural populations of the scum‐forming cyanobacteria Oscillatoria, Spirulina, and Microcystis showed a diel vertical migration pattern in fishponds. This migration was governed by two factors, light and nitrogen. Light penetrates only into the upper layers of these shallow waterbodies, while combined nitrogen is detectable only in the pond sediment. Migration between these two layers was correlated with cellular carbohydrate accumulation at the top layer and its degradation at the bottom. Gas‐vacuole collapse was not mediated by turgor pressure and gas vesicles re‐formed at an extremely slow rate. Thus it seems likely that a change in ballast due to carbohydrate loading at the lighted surface and unloading at the dark, nitrogen‐rich bottom actively influences vertical migration.
Proteomic analysis of anti‐angiogenic effects by a combined treatment with vinblastine and rapamycin in an endothelial cell line (2006)

Angiogenesis controls the new blood supply routes into the tumor mass via the host endothelial cells (ECs). In this study, the EA.hy926 endothelial cell line has been treated with vinblastine (VBL) and rapamycin (RAP), both separately and in combination at low doses. Recently, we demonstrated the synergistic antiangiogenic effects of a combination of VBL and RAP at very low doses in vitro and in vivo. Herein, we confirm the ability of this combined treatment to statistically inhibit the proliferation of ECs, in a synergistic manner, by inducing apoptosis. The aim of this study was to substantiate these findings at the protein level. Differential proteomic analysis was performed on untreated control cells, treated with VBL, incubated with RAP, or subjected to a drug combination. Differentially expressed 113 polypeptide chains were visualized and 65 were identified via MALDI-TOF analysis. Some of the regulated proteins are involved in the processes of angiogenesis, proliferation, migration, and apoptosis. The down-modulation of ATP synthase, annexin A2, heat shock p70, glucose-6-phosphate dehydrogenase, vasodilator-stimulated phosphoprotein, proteasome 26S, tryptophanyl-tRNA synthetase, and stathmin/OP18, as well as the up-modulation of carbonyl reductase, Rho-GDI, and histone H1.0 correlates with the synergistic antiangiogenic activity of VBL and RAP.
Model adaptation with least-squares SVM for adaptive hand prosthetics (2009)

The state-of-the-art in control of hand prosthetics is far from optimal. The main control interface is represented by surface electromyography (EMG): the activation potentials of the remnants of large muscles of the stump are used in a non-natural way to control one or, at best, two degrees-of-freedom. This has two drawbacks: first, the dexterity of the prosthesis is limited, leading to poor interaction with the environment; second, the patient undergoes a long training time. As more dexterous hand prostheses are put on the market, the need for a finer and more natural control arises. Machine learning can be employed to this end. A desired feature is that of providing a pre-trained model to the patient, so that a quicker and better interaction can be obtained. To this end we propose model adaptation with least-squares SVMs, a technique that allows the automatic tuning of the degree of adaptation. We test the effectiveness of the approach on a database of EMG signals gathered from human subjects. We show that, when pre-trained models are used, the number of training samples needed to reach a certain performance is reduced, and the overall performance is increased, compared to what would be achieved by starting from scratch.
Bioaugmentation for site remediation 3(3) (1995)

The articles in the collection discuss genetic engineering, nutritional amendments, the introduction of nonindigenous microorganisms, and other bioaugmentation techniques for enhancing biological activity to promote remediation of contaminated sites. Included are laboratory and field studies of the biological processes connected with in situ remediation of soil and water at sites contaminated with petroleum hydrocarbons, PAHs, PCP, TNT, insecticides, and chlorinated solvents. Site characteristics (e.g., soil porosity, nutrient availability) and methods for determining when bioaugmentation is needed are described. Among the bioaugmentation methods discussed are biodehalogenation systems, microbial filters, microbial ecapsulation, fertilization, niche adjustment, inoculation with laboratory-cultured and -adapted microorganisms, and the use of mixed fungi and bacteria.
Optic Neuropathy Due to Microbead-Induced Elevated Intraocular Pressure in the Mouse (2010)

Anterior chamber injection of microbeads effectively induced IOP elevation and glaucomatous optic neuropathy in mice. Development of an inducible mouse model of elevated IOP will allow applications of mouse genetic technology to the investigation of the mechanisms and the evaluation of treatment strategies of glaucoma.
Application of 46 kV, 100 MVA smart predictive line controller (SPLC) to AC electric arc furnace (1999)

The voltage flicker emission levels caused by electric arc furnace operation can cause objectionable disturbances in residential, incandescent lamp based light sources. Installation of a newly designed SPLC (smart predictive line controller) beta unit in the arc furnace power supply system of Co-Steel Lasco (46 kV, 100 MVA) reduced the flicker levels at the source. The paper presents the SPLC system description, details of installation, current and voltage fluctuation profiles, flicker measurements and operational benefits.
CCD linear image sensor with buried overflow drain structure (1981)

A CCD linear image sensor with buried overflow drain structure has been developed. Since the overflow drain, i.e. a reverse biased n-region buried under photosites arrayed in p-layer, makes an effective sink of excess electrons, imaging characteristics such as uniformity of photosensitivity, spectral response, resolution, and antiblooming, are highly improved.
Black Pepper (2010)

In Brief The black pepper (Piper nigrum L) vine and its extracts have been used as a folk medicine in a variety of cultures and are the source of the most commonly used spice worldwide. The chemical piperine is a major bioactive component present in black pepper (and white pepper as well) that has numerous reported physiological and drug-like actions. The scientific literature provides evidence that black pepper may have health benefits, particularly in enhancing digestive tract function. There is suggestive evidence that black pepper piperine may have nervous system benefits and may influence body energy usage in rats. Preliminary evidence in cell culture studies suggests that black pepper contains antioxidant constituents and possesses anti-inflammatory and antimicrobial properties. An overview of major uses for black pepper is presented here, and the strength of the evidence is graded The lowdown on a popular spice that adds zest to meals