gebhart/patrepeval huggingface dataset:
Proximity Extension Assay in Combination with Next-Generation Sequencing for High-throughput Proteome-wide Analysis (2021)

Understanding the dynamics of the human proteome is crucial for developing biomarkers to be used as measurable indicators for disease severity and progression, patient stratification, and drug development. The Proximity Extension Assay (PEA) is a technology that translates protein information into actionable knowledge by linking protein-specific antibodies to DNA-encoded tags. In this report we demonstrate how we have combined the unique PEA technology with an innovative and automated sample preparation and high-throughput sequencing readout enabling parallel measurement of nearly 1500 proteins in 96 samples generating close to 150,000 data points per run. This advancement will have a major impact on the discovery of new biomarkers for disease prediction and prognosis and contribute to the development of the rapidly evolving fields of wellness monitoring and precision medicine.
ChemInform Abstract: DIE VERW. VON N(ALPHA),N(IM)‐BIS‐(TERT.‐BUTYLOXYCARBONYL)‐HISTIDIN UND N(ALPHA)‐2‐(P‐BIPHENYLYL)‐ISOPROPYLOXYCARBONYL‐N(IM)‐TERT.‐BUTYLOXYCARBONYL‐HISTIDIN BEI DER FESTPHASEN‐SYNTH. VON HISTIDIN‐ENTHALTENDEN PEPTIDEN (1972)

Abstract Das Heptapeptid (I), das der Aminosäuresequenz 17‐23 des menschlichen Wachstumhormons entspricht, wird nach der Festphasenmethode a) unter Verwendung des Histidin‐Derivats (IIa) b) unter Verwendung des Histidin‐ Derivats (IIb) schrittweise synthetisiert.
Endoscopy of the Spinal Cord: Cadaveric Study and Clinical Experience (1999)

Recent improvements in instruments permit endoscopic examination of previously inaccessible sites. We report on the clinical use of a small-diameter endoscope to examine the spinal subarachnoid space, cord surface and syrinx cavities. Prior to clinical application, three types of endoscopes with external diameters of 0.5, 1.4 or 2.2 mm were inserted percutaneously in the lumbar region of five cadavers for preclinical evaluation of the procedure and the three endoscopes. The observations permitted us to perform spinal endoscopy preoperatively or intraoperatively using the 0.5-mm instrument in seven patients with spinal cord lesions between 1995 and 1997. The patients included two with spinal cord herniation through a dural defect, two with syringomyelia, one with spinal arachnoid cyst, one with spinal epidural cyst and one undergoing lumboperitoneal shunt for hydrocephalus. In patients in whom an endoscope was used preoperatively, the endoscope provided morphological information useful in preoperative diagnosis and planning surgical strategy. When the endoscope was used intraoperatively, areas outside the field of vision of a microscope could be examined, and physiological evaluation could include visualizing improved cord perfusion from the spinal subarachnoid space after surgery. Endoscopes could be safely inserted and approached to the lesions under direct vision while avoiding blood vessels and nerve roots on the spinal cord surface. No changes in symptoms or complications occurred in association with endoscopy. Using a small-diameter endoscope, the contents of the spinal subarachnoid space could be examined. Further improvements to increase possible endoscopic manipulation and enhance safety may extend the possibilities for endoscopic examination and permit endoscopic treatment.
Transformative Heterointerface Evolution and Plasmonic Tuning of Anisotropic Trimetallic Nanoparticles (2017)

Multicomponent nanoparticles that incorporate multiple nanocrystal domains into a single particle represent an important class of material with highly tailorable structures and properties. The controlled synthesis of multicomponent NPs with 3 or more components in the desired structure, particularly anisotropic structure, and property is, however, challenging. Here, we developed a polymer and galvanic replacement reaction-based transformative heterointerface evolution (THE) method to form and tune gold-copper-silver multimetallic anisotropic nanoparticles (MAPs) with well-defined configurations, including structural order, particle and junction geometry, giving rise to extraordinarily high tunability in the structural design, synthesis and optical property of trimetallic plasmonic nanoantenna structures. MAPs can easily, flexibly integrate multiple surface plasmon resonance (SPR) peaks and incorporate various plasmonic field localization and enhancement within one structure. Importantly, a heteronanojunction in these MAPs can be finely controlled and hence tune the SPR properties of these structures, widely covering UV, visible and near-infrared range. The development of the THE method and new findings in synthesis and property tuning of multicomponent nanostructures pave ways to the fabrication of highly tailored multicomponent nanohybrids and realization of their applications in optics, energy, catalysis and biotechnology.
Palatability and Muscle Properties of Beef as Influenced by Preslaughter Growth Rate (1985)

Thirty-six Hereford-Angus yearling steers, average weight 247 kg, were assigned to one of three feeding regimens for 120 d. Treatment 1 (T-1) steers were fed ad libitum to obtain maximal weight gain; average daily gain (ADG) was 1.42 kg. Treatment 2 (T-2) steers were restricted to an ADG of .77 kg. Treatment 3 (T-3) steers were restriced to an ADG of .34 kg. Postslaughter chilling was slowed in the left sides of carcasses from T-2 and T-3 cattle so that the rate of temperature decline in the longissimus (LD) muscle matched that of the fatter, heavier carcasses from T-1. A trained sensory panel rated steaks from the LD and semimembranosus (SM) muscles of T-1 steers more tender (P<.05) than those from the left side of T-2 and T-3. The SM from T-3 was less (P<.05) tender than that from T-2. Significant tenderness differences among treatments were not detected with Warner-Bratzler shear, although the ranking among treatments was the same as obtained with sensory analysis. Lower tenderness scores of T-3 SM were associated with higher (P<.05) intramuscular collagen thermal-shrinkage temperature, lower (P<.05) percentage soluble collagen and slightly higher (P>.05) total collagen. Plasma nonprotein hydroxyproline concentrations were lower in T-3 steers throughout the feeding period. Lower tenderness in the LD of the T-3 group may have reflected the shorter sarcomeres in this muscle than in that from either T-1 or T-2, even though carcasses were chilled at similar rates. Myofibril fragmentation did not differ among treatments. Results show that growth rate before slaughter affects beef tenderness, a relationship that may be mediated partly through the turnover and(or) maturation rate of intramuscular collagen and also through effects of growth rate on sarcomere length.
PP.16.33 (2015)

Objective: Cardiovascular diseases still become the leading cause of death in the world. All over the world, there were approximately 50 million deaths every year caused by cardiovascular diseases. Hypertension is known as one of cardiovascular diseases that frequently happened. There are so many factors related to its pathogenesis. One of which is magnesium intracellular ion change. Up to now, studies and researched related to that topic are still being conducted to acknowledge magnesium supplementation for reducing blood pressure. Design and method: Structured PubMed search was conducted that yielded 34 articles, where after the inclusion and exclusion criteria were applied, 8 articles remained. After reading the full texts, 3 articles were appraised concerning its validity, relevance, and applicability. Results: From 3 articles appraised, 410 mg/day magnesium supplementation could reduce blood pressure and mortality of cardiovascular disease. Conclusions: 410 mg/day magnesium supplementation could reduce blood pressure and mortality of cardiovascular disease.
Probing the minimal scattering events in enhanced backscattering of light in discrete random media (2005)

We exploit low spatial coherence illumination to dephase time-reversed partial waves outside its finite coherence area, which virtually creates a controllable coherence volume and isolates the minimal number of scattering events from higher order scattering in enhanced backscattering (EBS, also known as coherent backscattering) of light. We report the first experimental evidence that the minimum number of scattering events in EBS is double scattering in discrete random media, which has been hypothesized since the first observation of EBS of light. We discuss several unique characteristics and potential applications of low-coherence EBS in weakly scattering random media.
Pericyte-Coverage of Human Tumor Vasculature and Nanoparticle Permeability (2012)

Nano drug delivery systems (nanoDDS) are a promising strategy for treatment of human tumors. As indicated by our previous work, the extent of pericyte-coverage of tumor vasculature is important to determining nanoDDS efficacy since intratumoral accumulation of nanoDDS is less in tumor models with pericyte-covered vasculature. Here we investigated the clinical relevance of our previous observations in animal models, by determining pericyte coverage using immunohistochemistry of smooth muscle actin (SMA) with CD34: a vascular endothelial marker, in human tumor tissue samples. The investigation revealed that tumor vasculature coverage by pericytes in pancreatic and diffuse-type gastric cancers was significantly greater than in ovarian, colon, and intestinal-type gastric cancers. The latter group of cancers is easier to treat clinically. These observations are consistent with our previous findings in animal models. On the basis of these findings we believe optimization of nanoDDS delivery should be done depend upon a clear understanding of the effects of pericyte vascular coverage.
Rituali civici a Siena in età medievale. Ipotesi sulla processione dei ceri e dei censi come elemento aggregante della compagine territoriale (secoli XII-XIV) (2016)

Sulla base di numerosi riferimenti documentari, il saggio analizza l'evoluzione e i vari significati della processione dell'offerta di ceri e censi alla cattedrale senese in età basso medievale, valutandone gli aspetti antropologici.
Genetic, enzymatic, and pathogenic studies of the iron superoxide dismutase of Campylobacter jejuni (1994)

Campylobacter jejuni is a microaerobic bacterium that produces an acute, self-limiting, watery or bloody diarrhea in humans. Little is known about how C. jejuni causes disease or even what specific capabilities it requires for survival in vivo. The enzyme, superoxide dismutase (SOD), which catalyzes the breakdown of superoxide radicals to hydrogen peroxide and dioxygen is one of the bacterial cell's major defense mechanisms against oxidative damage. A PCR-based search for sod genes in C. jejuni 81-176 revealed that this bacterium contained at least one sod gene. We cloned and sequenced a sod gene from 81-176 and determined that its predicted protein product was most similar to that of FeSODs (sodB genes). Transcriptional analysis indicated that this gene is monocistronic and may be transcribed from a sigma 70-like promoter. Nondenaturing polyacrylamide gels stained to reveal SOD activities, accompanied by inhibition studies, demonstrated that C. jejuni produces five electrophoretically distinct bands of SOD activity, all of which appeared to be FeSODs. Analysis of an 81-176 sodB strain revealed that all of these FeSOD activities may be products of the one sodB gene that we cloned. The expression and enzymatic activity of the respective sodB and FeSOD produced by both C. jejuni and Helicobacter pylori were examined in Escherichia coli. Both genes were expressed in E. coli, and the proteins produced were enzymatically active. Finally, the ability of the 81-176 sodB strain to survive INT407 cell invasion was found to be significantly decreased (12-fold) compared with that of the parent, suggesting a potential role for SodB in C. jejuni intracellular survival.
Benefits of NOPO As Chelator in Gallium-68 Peptides, Exemplified by Preclinical Characterization of<sup>68</sup>Ga-NOPO–c(RGDfK) (2014)

The αvβ3-integrin addressing cyclic pentapeptide cyclo(RGDfK) was conjugated to NOPO, 1,4,7-triazacyclononane-1,4-bis[methylene(hydroxymethyl)phosphinic acid]-7-[methylene(2-carboxyethyl)phosphinic acid], a bifunctional chelator with exceptional gallium-68 labeling properties. NOPO-c(RGDfK) and its Ga(III) and Cu(II) complexes showed high affinity to αvβ3 integrin (IC50 = 0.94 ± 0.06, 1.02 ± 0.09, and 0.51 ± 0.06 nM, respectively). (68)Ga labeling of NOPO-c(RGDfK) in an automated GMP-compliant procedure was performed with near-quantitative radiochemical yield, using precursor amounts as low as 0.5 nmol (approximately 0.6 μg). (68)Ga-NOPO-c(RGDfK) was obtained with high purity (>99% by radio-HPLC/TLC) and, optionally, could be produced with specific activities up to 6 TBq/μmol. M21/M21L (human melanoma with high/low αvβ3 integrin expression) xenografted athymic CD-1 nude mice were used for biodistribution, in vivo stability studies, and PET imaging. (68)Ga-NOPO-c(RGDfK) showed rapid and specific uptake in M21 tumor xenografts (2.02 ± 0.34% ID/g at 60 min p.i.) and was found stable in vivo. Its high hydrophilicity is reflected by an octanol-water distribution coefficient (log D = -4.6) which is more than 1 order of magnitude lower compared to respective NOTA or DOTA analogues. As expected, (68)Ga-NOPO-c(RGDfK) thus showed fast renal clearance from nontargeted tissues. We conclude that NOPO might generally prove a useful means to improve renal clearance of corresponding radiopharmaceuticals by increasing the polarity of its bioconjugates. Favorable labeling properties render NOPO conjugates highly recommendable for reliable routine production of (68)Ga-radiopharmaceuticals in a clinical setting.
Hybrid methods for analysis of complex scatterers (1989)

Depending on the angle of illumination, electrically large scatterers can support a variety of electromagnetic (EM) phenomena, such as traveling waves, creeping waves, and edge/surface diffraction effects. The electrical size of a body limits the tractability of numerical methods such as the method of moments (MM), and the geometric complexity of an object circumscribes the applicability of optics-derived methods. Hybrid methods incorporating both numerical and high-frequency asymptotic techniques have the potential to substantially enlarge the class of EM scattering problems that can be treated. In this discussion, the current-based hybrid formulation is summarized for classes of two- and three-dimensional scatterers. The use of Ansatz solutions derived from physical optics, the physical theory of diffraction, and the Fock theory is illustrated for perfectly conducting, partially penetrable, and totally coated bodies. For the latter, a generalization rooted in the impedance boundary (Leontovich) condition is used. Complementing these Ansatz solutions, the Galerkin representation is used for regions where the foregoing are computationally or physically intractable. The above cases are illustrated by representative solutions explicating the approach.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Institutional framework for integrated Pharmaceutical Benefits Management: results from a systematic review (2015)

While most European Union Member States promote the implementation of selected e-Health tools, like e-Prescribing, these efforts do not necessarily implement an integrated package. There is no single agent who would manage an insured patients' access to medicines and health care in a coordinated manner, thereby increasing the efficiency and safety of drug policy. More attention should be paid by European Union Member States as to how to integrate various e-Health tools to enhance benefits to both individuals and societies. One solution could be to implement an integrated "pharmacy benefit management" model, which is well established in the USA and Canada and provides an integrated package of cost-containment methods, implemented within a transparent institutional framework and powered by strong motivation of the agent.
A differential semblance criterion for inversion of multioffset seismic reflection data (1993)

Mean square error leading to least squares inversion of multioffset reflection seismograms is insensitive to velocity trend information except in the immediate vicinity of a kinematically correct model. In contrast, differential semblance retains sensitivity to velocity trend changes over a wide range of models. The differential semblance criterion combines mean square error with the mean square differences of inverted models from data sets at neighboring shot positions (or offsets, or slownesses, …). Differential semblance compares model estimates at nearby acquisition parameters which are similar even when the model velocity trends are incorrect. Because the method inverts the data, so that the estimated model amplitudes are meaningful, simple differences between the (unstacked) model estimates give a reliable measure of velocity error. A mathematical investigation indicates that the differential semblance criterion is smooth and convex over a large range of velocity models. Numerical simulation using synthetic data sets verifies this contention.
Real-Time Quantification and Display of Skin Radiation During Coronary Angiography and Intervention (2001)

Monitoring of the dose distribution at the skin will alert the operator to the development of high-dose areas; by use of other gantry settings with nonoverlapping entrance fields, different generator settings, and extra collimation, skin lesion can be avoided.
CD40‐activated B cells contribute to mesothelioma tumor regression (2010)

Targeting CD40, a member of the tumor necrosis factor superfamily, using agonist antibodies (Abs) produces dramatic antitumor effects. Indeed, high-dose intravenous anti-CD40 Ab 'licenses' dendritic cells (DCs) that instruct activated CD8(+) cytotoxic T cells to leave lymph nodes (LNs) and penetrate the mesothelioma tumor microenvironment. However, toxic side effects and the potential of an 'overwhelmed' immune response warrant an alternative approach. In this study, we show that injecting lower doses of anti-CD40 Ab directly into the tumor bed avoided toxic side effects and prolonged survival in 60% of mice, with most cured. Unexpectedly, DCs in tumors and LNs 'disappeared', CD8(+) tumor-specific T-cell numbers and function were not enhanced, and T cells did not infiltrate regressing tumors. CD4(+) or CD8(+) depletion only marginally hindered anti-CD40 Ab efficacy implying another effector mechanism. B-cell numbers significantly increased in tumors, draining LNs and spleens during intratumoral anti-CD40 Ab treatment. CD40 targeting had no effect on splenic B-1 cells, obliterated marginal zone B cells and promoted follicular (FO) B-cell activity. Adoptive transfer of tumor antigen-experienced, CD40-activated B cells, or their immunoglobulin products, which recognized autoantigens on mesothelioma cells, protected against tumor challenge. Finally, studies using B-cell knockout mice showed that successful treatment of established tumors required the presence of B cells. Thus, these data suggest that CD40-activated FO B cells can become an important component of an effective antitumor immune response.
Hardware support for code integrity in embedded processors (2005)

Computer security becomes increasingly important with continual growth of the number of interconnected computing platforms. Moreover, as capabilities of embedded processors increase, the applications running on these systems also grow in size and complexity, and so does the number of security vulnerabilities. Attacks that impair code integrity by injecting and executing malicious code are one of the major security issues. This problem can be addressed at different levels, from more secure software and operating systems, down to solutions that require hardware support. Most of the existing techniques tackle the problem of security flaws at the software level, but this approach lacks generality and often induces prohibitive overhead in performance and cost, or generates a significant number of false alarms. On the other hand, a further increase in the number of transistors on a single chip enables integrated hardware support for functions that formerly were restricted to the software domain. Hardware-supported defense techniques have the potential to be more general and more efficient than solely software solutions. This paper proposes four new architectural extensions to ensure complete run-time code integrity using instruction block signature verification. The experimental analysis shows that the proposed techniques have low performance and energy overhead. In addition, the proposed mechanism has low hardware complexity, and does not impose either changes to the compiler or changes to the existing instruction set architecture.
Introduction to linear regression analysis : solutions manual to accompany (2013)

Praise for the Fourth Edition As with previous editions, the authors have produced a leading textbook on regression. -- Journal of the American Statistical Association A comprehensive and up-to-date introduction to the fundamentals of regression analysis Introduction to Linear Regression Analysis, Fifth Edition continues to present both the conventional and less common uses of linear regression in today's cutting-edge scientific research. The authors blend both theory and application to equip readers with an understanding of the basic principles needed to apply regression model-building techniques in various fields of study, including engineering, management, and the health sciences. Following a general introduction to regression modeling, including typical applications, a host of technical tools are outlined such as basic inference procedures, introductory aspects of model adequacy checking, and polynomial regression models and their variations. The book then discusses how transformations and weighted least squares can be used to resolve problems of model inadequacy and also how to deal with influential observations. The Fifth Edition features numerous newly added topics, including: A chapter on regression analysis of time series data that presents the Durbin-Watson test and other techniques for detecting autocorrelation as well as parameter estimation in time series regression models Regression models with random effects in addition to a discussion on subsampling and the importance of the mixed model Tests on individual regression coefficients and subsets of coefficients Examples of current uses of simple linear regression models and the use of multiple regression models for understanding patient satisfaction data. In addition to Minitab, SAS, and S-PLUS, the authors have incorporated JMP and the freely available R software to illustrate the discussed techniques and procedures in this new edition. Numerous exercises have been added throughout, allowing readers to test their understanding of the material, and a related FTP site features the presented data sets, extensive problem solutions, software hints, and PowerPoint slides to facilitate instructional use of the book. Introduction to Linear Regression Analysis, Fifth Edition is an excellent book for statistics and engineering courses on regression at the upper-undergraduate and graduate levels. The book also serves as a valuable, robust resource for professionals in the fields of engineering, life and biological sciences, and the social sciences.