gebhart/patrepeval huggingface dataset:
Regional assignment of the structural gene for human alpha-L-iduronidase. (1984)

The structural gene encoding human alpha-L-iduronidase has been assigned to chromosome 22 by using immunologic, electrophoretic, and somatic cell hybridization techniques. Polyclonal rabbit antibodies raised against purified human low-uptake alpha-L-iduronidase were used to discriminate the human and murine isozymes by a sensitive immuno-precipitation assay. The human chromosome constitution of each clone was determined by cytogenetic and enzyme marker electrophoretic techniques. In 65 human (fibroblast)-mouse (RAG) somatic cell hybrids (from four independent fusions), the expression of human alpha-L-iduronidase was 100% concordant with the presence of human chromosome 22; the assignment was confirmed by the demonstration of the human enzyme in tertiary somatic cell hybrids containing only chromosome 22. Further verification of the gene assignment was made by detection of the human enzyme in tertiary chromosome 22 positive hybrids by Ouchterlony immunodiffusion and rocket immunoelectrophoretic experiments with polyclonal anti-human alpha-L-iduronidase antibodies that were monospecific for the human enzyme. Expression of human enzymatic activity in chromosome 22 positive hybrid lines was markedly reduced; for example, a tertiary hybrid (R-G21-J-15), which contained an average of 1.7 chromosome 22s per cell, only had about 15% of the activity detected in normal diploid fibroblasts. Immunologic studies suggested that the reduced expression was due to abnormal post-translational processing or aggregation (or both) of the human and murine isozymes in these hybrids. Regional assignment of the human structural gene to 22pter----q11 was accomplished by gene dosage studies using diploid human fibroblast lines that were partially monosomic or trisomic for chromosome 22.
Analyzing PCBs (1986)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAnalyzing PCBsAnn L. Alford-StevensCite this: Environ. Sci. Technol. 1986, 20, 12, 1194–1199Publication Date (Print):December 1, 1986Publication History Published online1 May 2002Published inissue 1 December 1986https://pubs.acs.org/doi/10.1021/es00154a001https://doi.org/10.1021/es00154a001research-articleACS PublicationsRequest reuse permissionsArticle Views467Altmetric-Citations41LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Would Scaling to Extreme Ultraviolet or Soft X-Ray Communications Resolve the Capacity Crunch? (2018)

Optical networks are facing fundamental capacity scalability problems owing to Shannon limits in silica fiber, frequently summarized as the “optical networks capacity crunch.” We explore the possibility of scaling communication capacities through the use of significantly higher carrier frequencies, such as the extreme ultraviolet or the soft x-ray spectral region, in analogy to the 3-7 orders of magnitude increase in carrier frequencies when optical fiber replaced microwave transmission technologies in the late 1970s. We find that only very limited capacity gains on the order of 10 may be achieved relative to the 1550-nm telecommunications band without fundamentally increasing a system's received energy per bit, its relative bandwidth, or its degree of spatial parallelism. Conversely, we predict the required reduction of waveguide losses that would be needed to make higher carrier frequencies a viable proposition from an energy efficiency point of view. Our analyses include the use of as of yet unexplored quantum communication techniques, whose required energy per bit we find to be lower-bounded by kT/log <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> e at any carrier frequency, which may have implications beyond the communications applications discussed in this paper.
Improved production of propionic acid using genome shuffling (2016)

Abstract Traditionally derived from fossil fuels, biological production of propionic acid has recently gained interest. Propionibacterium species produce propionic acid as their main fermentation product. Production of other organic acids reduces propionic acid yield and productivity, pointing to by‐products gene‐knockout strategies as a logical solution to increase yield. However, removing by‐product formation has seen limited success due to our inability to genetically engineer the best producing strains (i.e. Propionibacterium acidipropionici ). To overcome this limitation, random mutagenesis continues to be the best path towards improving strains for biological propionic acid production. Recent advances in next generation sequencing opened new avenues to understand improved strains. In this work, we use genome shuffling on two wild type strains to generate a better propionic acid producing strain. Using next generation sequencing, we mapped the genomic changes leading to the improved phenotype. The best strain produced 25% more propionic acid than the wild type strain. Sequencing of the strains showed that genomic changes were restricted to single point mutations and gene duplications in well‐conserved regions in the genomes. Such results confirm the involvement of gene conversion in genome shuffling as opposed to long genomic insertions.
Block Equalization and Generalized MLSE Arbitration for the HSPA WCDMA Uplink (2008)

High speed packet access (HSPA), the latest evolution of the wideband code division multiple access (WCDMA) standard, provides higher uplink data rates using higher-order modulation (16-QAM) and multicode transmission. Equalization is needed when the channel is dispersive. Despite gains from linear equalization, there is room for further improvement. In this paper, we consider two enhancements: block equalization with joint detection and a generalization of arbitration. Block equalization with joint detection, originally developed for non-spread systems, involves jointly detecting symbols from multiple symbol periods and using a feedback filter to remove interference from past symbol periods. Here we extend this idea to parallel symbol streams, add feedforward filtering, and introduce the notion of block linear equalization. Arbitration, also originally developed for nonspread systems, involves bidirectional decision feedback equalization and selection. Here we generalize the arbitration concept to other forms of equalization and improve the selection process using constrained maximum-likelihood sequence estimation (MLSE). These enhancements are then evaluated via simulation for the HSPA uplink, demonstrating their efficacy.
SARS-CoV-2: is there neuroinvasion? (2021)

While SARS-CoV-2 is detected in brains from severely infected patients, it is unclear on how it gets there. There is no sound evidence of SARS-CoV-2 flux into brain to significantly contribute to the overall outcomes once the respiratory system is invaded by the virus. The consensus, based on the normal route of infection and presence of SARS-CoV-2 in severely infected patients, is that the olfactory mucosa is a possible route into brain. Studies are needed to demonstrate flux of SARS-CoV-2 into brain, and its replication in the parenchyma to demonstrate neuroinvasion. It is possible that the neurological manifestations of COVID-19 are a consequence of mainly cardio-respiratory distress and multiorgan failure. Understanding potential SARS-CoV-2 neuroinvasion pathways could help to better define the non-respiratory neurological manifestation of COVID-19.
Study on IL-6 in Postmenopausal Women with Sjgren's Syndrome (2006)

Objective: To investigate the role of interleukin-6 (IL-6) in the pathogenesis of primary Sjogren's syndrome (pSS). Methods: Serum and saliva specimen of twenty-one postmenopausal patients with pSS(the pSS group) and twenty-one postmenopausal healthy women(the control group) were collected. Enzyme linked immunosorbent assay (ELISA) were used to determine levels of IL-6 then IgG, IgA, IgM and ESR were measured by routine methods in our hospital. Results: The serum and saliva level of IL-6 in pSS patients were higher than that in the control group (P 0.01). The serum and saliva level of IL-6 in the pSS group had linear positive correlation with IgG and ESR in serum (r=0.699, 0.757 and 0.546,0.544 t=4.261, 5.050 and 2.841,and 2.826 respectively, P 0.01 or P 0.05, but had no linear correlation with IgA and IgM in serum (r=0.392, 0.412 and 0.393, 0.291, t=1.857, 1.971 and 1.863,1.326 respectively, P 0.05). The serum and saliva level of IL-6 in pSS patients had linear (r=0.649, t=3.718, P 0.01). Conclusion: IL-6 is an important factor on the pathogenesis and perpetuation of pSS by inducing IgG synthesis and secretion. The IL-6 level in saliva which may be a indicator of that in serum can be used to evaluate the activity of pSS.
Graphene-supported silver nanoparticles for pH-neutral electrocatalytic oxygen reduction (2013)

In order to improve the electrocatalysis of oxygen reduction and reduce the captive cost of microbial fuel cell (MFC), graphene-supported catalyst of silver nanoparticles (Ag/RG) was prepared and its activity toward oxygen reduction reaction (ORR) in pH-neutral condition was examined. The Ag/RG catalyst was prepared by chemical reduction of aqueous silver ions to silver nanoparticles on the surface of reduced graphene (RG), which was obtained from graphene oxide (GO) precursor synthesized via classic Hummer's method using graphite as starting material. The results demonstrated that aqueous co-reduction method could yield high-quality Ag/RG catalyst, in which Ag nanoparticles were dispersed on the graphene surface with average diameter of 13 nm as revealed by XRD and TEM examination. The XPS results suggested the presence of polar oxygen-containing functional groups on the graphene sheets could provide the nuclei to form Ag nano-structure. The CV results showed that Ag/RG was active of catalyzing ORR electrochemically in pH-neutral electrolyte. Since Ag costs much less than Pt, Ag/RG is expected to hold remarkable potential as a promising alternative to promote the commercialization of MFC for practical application.
Bacterial Injectisomes: Needle Length Does Matter (2005)

Many pathogenic bacteria use a type III secretion nanomachine (an injectisome) to deliver virulence proteins into the cytosol of their eukaryotic host cells. Most injectisomes possess a stiff needlelike structure of a genetically defined length. We found that a minimal needle length was required for efficient functioning of the Yersinia enterocolitica injectisome. This minimal needle length correlated with the length of the major adhesin at the bacterial surface. The needle may be required for triggering type III secretion, and its length may have evolved to match specific structures at the bacterial and host cell surfaces.
FisheyeSuperPoint: Keypoint Detection and Description Network for Fisheye Images (2022)

Keypoint detection and description is a commonly used building block in computer vision systems particularly for robotics and autonomous driving.However, the majority of techniques to date have focused on standard cameras with little consideration given to fisheye cameras which are commonly used in urban driving and automated parking.In this paper, we propose a novel training and evaluation pipeline for fisheye images.We make use of SuperPoint as our baseline which is a self-supervised keypoint detector and descriptor that has achieved state-of-the-art results on homography estimation.We introduce a fisheye adaptation pipeline to enable training on undistorted fisheye images.We evaluate the performance on the HPatches benchmark, and, by introducing a fisheye based evaluation method for detection repeatability and descriptor matching correctness, on the Oxford RobotCar dataset.
Analysis of a reset-free polarization controller for fast automatic polarization stabilization in fiber-optic transmission systems (1994)

We analyze the operation of a reset-free polarization controller comprising three endlessly rotatable wave plates: a first quarter-wave plate followed by a half-wave plate and a second quarter-wave plate, which is rotated synchronously with the first quarter-wave plate. We show that for any arbitrary angular offset between the two quarter-wave plates; the controller allows continuous, reset-free transformations from any varying general input state of polarization into any general output state. An integrated-optic realization of this scheme on z-propagation LiNbO/sub 3/ offers control speeds that well exceed the speed of natural polarization fluctuations in standard single-mode fibers, thus allowing fast automatic polarization stabilization in fiber-optic transmission systems.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Digital spectra of nonuniformly sampled signals: fundamentals and high-speed waveform digitizers (1988)

A digital spectral representation of a nonuniformly sampled signal is derived, and a spectrum analysis of a nonuniformly sampled sinusoid is presented. It is found that the spectrum of a nonuniformly sampled sinusoid comprises uniformly spaced line spectra; in addition, the signal-to-noise ratio is obtained in closed form. The theories are then applied to analyze the harmonic distortion introduced in high-speed waveform digitizers due to time-base errors. Specifically, waveform digitizers are analyzed which utilize interleaving/multiplexing and random equivalent time-sampling techniques and the monolithic A/D converter technology to extend their capabilities. Theoretical results are confirmed by experimental results with a real waveform digitizer.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
The Wing 2 Region of the FOXC1 Forkhead Domain Is Necessary for Normal DNA-Binding and Transactivation Functions (2004)

Biochemical analysis of mutations G165R and R169P and of a previously reported mutation, M161K, demonstrate the functional significance of wing 2. M161K and R169P disrupt DNA binding of FOXC1, consistent with the hypothesis that wing 2 is necessary for DNA binding. The results also suggest that wing 2 plays a role in gene activation. These results provide the first insights into how mutations in wing 2 disrupt FOXC1 function.
Mycoplasmas produce double‐stranded ribonuclease (1990)

Mycoplasmas (Mollicutes) constitute a constant threat as insidious contaminants of animal cell cultures. They are responsible for myriad biochemical reactions associated with the cells they infect, and undoubtedly have been the source of metabolic and physiological activities attributed to their hosts. In an attempt to demonstrate a dsRNA-inducible double-stranded ribonuclease (dsRNase) in mammalian cells, comparable to that reported in avian cells, we discovered high levels of dsRNase "induced" by a particular stock of vesicular stomatitis virus. We now report that the double-stranded ribonuclease resulted from the activity of a contaminant in that stock--a "noncultivable" Mycoplasma hyorhinis. This report demonstrates the ubiquitous distribution of dsRNase among mycoplasmas, presents some characteristics of the enzyme and its production, and implicates once again mycoplasmas as contaminants of cell culture and potential perturbers of cellular physiology.
Magnetic and magneto-optic properties of amorphous GdFeBi-films (invited) (1979)

Amorphous rare-earth-transition metal alloys of composition (Gd1−xFex)1−yBiy with 0?y?0.17 have been prepared by high vaccum electron gun evaporation onto rotating glass substrates. The magnetic and magneto-optic properties have been investigated in the temperature range 4.2 K?T?TC. From the temperature dependence of the saturation magnetization the compensation temperature is found to decrease strongly with increasing y (Tcomp=0 for x=0.74, y=0.1) while the Curie temperature and the temperature for crystallization are much less affected. The magneto-optic Faraday and Kerr rotation display a considerable increase with increasing Bi-content. The temperature behavior can be interpreted in terms of the mean field analysis indicating a pronounced influence of the bismuth on the iron moment and the exchange constants.
Full-wave design of spurious free DR TE mode band pass filters (1995)

Analytic full-wave analysis and design of direct-coupled dielectric loaded bandpass filters in rectangular waveguide configuration using the TE/sub 10/ mode with a spurious-free response up to twice the operating frequency is described. The filter has no tuning screws and is small in size. Theoretical design of the filter is supported by the experimental results. Superiority of the filter over the inductive window-coupled filter is presented. Practical issues faced while building the filter are discussed. An alternative folded configuration of the filter that allows the implementation of both the canonical elliptic function as well as the Chebychev response is also presented.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Current Status on Therapeutic Molecules Targeting Siglec Receptors (2020)

The sialic acid-binding immunoglobulin-type of lectins (Siglecs) are receptors that recognize sialic acid-containing glycans. In the majority of the cases, Siglecs are expressed on immune cells and play a critical role in regulating immune cell signaling. Over the years, it has been shown that the sialic acid-Siglec axis participates in immunological homeostasis, and that any imbalance can trigger different pathologies, such as autoimmune diseases or cancer. For all this, different therapeutics have been developed that bind to Siglecs, either based on antibodies or being smaller molecules. In this review, we briefly introduce the Siglec family and we compile a description of glycan-based molecules and antibody-based therapies (including CAR-T and bispecific antibodies) that have been designed to therapeutically targeting Siglecs.
JAMS: Jitter-aware message scheduling for FlexRay automotive networks: Special session paper (2017)

FlexRay is becoming a popular in-vehicle communication protocol for the next generation x-by-wire applications such as drive-by-wire and steer-by-wire. The protocol supports both time-triggered and event-triggered transmissions. One of the important challenges with time-triggered transmissions is jitter, which is the unpredictable delay-induced deviation from the actual periodicity of a message. Failure to account for jitter can be catastrophic for time critical automotive applications. In this paper we propose a novel scheduling framework (JAMS) that handles both jitter affected time-triggered messages and high priority event-triggered messages to ensure message delivery while satisfying timing constraints. At design time, JAMS handles packing of multiple signals from Electronic Control Units (ECUs) into messages, and synthesizes a schedule using intelligent heuristics. At runtime, a Multi-Level Feedback Queue handles jitter affected time-triggered messages, and high priority event-triggered messages. We also propose a runtime scheduler that packs these messages into the FlexRay static segment slots depending on available slack. Experimental analysis indicates that JAMS improves the response time by 25.3% on average and up to 41% compared to the best-known prior work in the area.
Efficient Algorithms for Online Game Playing and Universal Portfolio Management. (2006)

We introduce a new algorithm and a new analysis technique that is applicable to a variety of online optimization scenarios, including regret minimization for Lipschitz regret functions, universal portfolio management, online convex optimization and online utility maximization. In addition to being more efficient and deterministic, our algorithm applies to a more general setting (e.g. when the payoff function is unknown). For the general online game playing setting it is the first to attain logarithmic regret, as opposed to previous algorithms attaining polynomial regret. The algorithm extends a natural online method studied in the 1950’s, called “follow the leader”, thus answering in the affirmative a conjecture about universal portfolios made by Cover and Ordentlich and independently by Kalai and Vempala. The techniques also leads to derandomization of an algorithm by Hannan, and Kalai and Vempala. Our analysis shows a surprising connection between interior point methods and online optimization by using the follow the leader method.
Genome-wide association analysis identifies multiple loci related to resting heart rate (2010)

Higher resting heart rate is associated with increased cardiovascular disease and mortality risk. Though heritable factors play a substantial role in population variation, little is known about specific genetic determinants. This knowledge can impact clinical care by identifying novel factors that influence pathologic heart rate states, modulate heart rate through cardiac structure and function or by improving our understanding of the physiology of heart rate regulation. To identify common genetic variants associated with heart rate, we performed a meta-analysis of 15 genome-wide association studies (GWAS), including 38,991 subjects of European ancestry, estimating the association between age-, sex- and body mass-adjusted RR interval (inverse heart rate) and approximately 2.5 million markers. Results with P < 5 × 10(-8) were considered genome-wide significant. We constructed regression models with multiple markers to assess whether results at less stringent thresholds were likely to be truly associated with RR interval. We identified six novel associations with resting heart rate at six loci: 6q22 near GJA1; 14q12 near MYH7; 12p12 near SOX5, c12orf67, BCAT1, LRMP and CASC1; 6q22 near SLC35F1, PLN and c6orf204; 7q22 near SLC12A9 and UfSp1; and 11q12 near FADS1. Associations at 6q22 400 kb away from GJA1, at 14q12 MYH6 and at 1q32 near CD34 identified in previously published GWAS were confirmed. In aggregate, these variants explain approximately 0.7% of RR interval variance. A multivariant regression model including 20 variants with P < 10(-5) increased the explained variance to 1.6%, suggesting that some loci falling short of genome-wide significance are likely truly associated. Future research is warranted to elucidate underlying mechanisms that may impact clinical care.
Deafness-Induced Changes in the Auditory Pathway: Implications for Cochlear Implants (2001)

A profound sensorineural hearing loss induces significant pathological and atrophic changes within the cochlea and central auditory pathway. We describe these deafness-induced morphological and functional changes following controlled lesions of the cochlea in experimental animals. Such changes are generally consistent with the limited number of reports describing deafness-induced changes observed in human material. The implications of these pathophysiological changes within the auditory pathway on cochlear implant function are discussed. Finally, the plastic response of the deafened auditory system to electrical stimulation of the auditory nerve is reviewed in light of the clinical implications for cochlear implant recipients.