gebhart/patrepeval huggingface dataset:
Wiring Photosystem I for Direct Solar Hydrogen Production (2009)

The generation of H(2) by the use of solar energy is a promising way to supply humankind's energy needs while simultaneously mitigating environmental concerns that arise due to climate change. The challenge is to find a way to connect a photochemical module that harnesses the sun's energy to a catalytic module that generates H(2) with high quantum yields and rates. In this review, we describe a technology that employs a "molecular wire" to connect a terminal [4Fe-4S] cluster of Photosystem I directly to a catalyst, which can be either a Pt nanoparticle or the distal [4Fe-4S] cluster of an [FeFe]- or [NiFe]-hydrogenase enzyme. The keys to connecting these two moieties are surface-located cysteine residues, which serve as ligands to Fe-S clusters and which can be changed through site-specific mutagenesis to glycine residues, and the use of a molecular wire terminated in sulfhydryl groups to connect the two modules. The sulfhydryl groups at the end of the molecular wire form a direct chemical linkage to a suitable catalyst or can chemically rescue a [4Fe-4S] cluster, thereby generating a strong coordination bond. Specifically, the molecular wire can connect the F(B) iron-sulfur cluster of Photosystem I either to a Pt nanoparticle or, by using the same type of genetic modification, to the differentiated iron atom of the distal [4Fe-4S].(Cys)(3)(Gly) cluster of hydrogenase. When electrons are supplied by a sacrificial donor, this technology forms the cathode of a photochemical half-cell that evolves H(2) when illuminated. If such a device were connected to the anode of a photochemical half-cell that oxidizes water, an in vitro solar energy converter could be realized that generates only O(2) and H(2) in the light. A similar methodology can be used to connect Photosystem I to other redox proteins that have surface-located [4Fe-4S] clusters. The controlled light-driven production of strong reductants by such systems can be used to produce other biofuels or to provide mechanistic insights into enzymes catalyzing multielectron, proton-coupled reactions.
One step forward (2007)

Subtitle: US airlines have turned in an admirable set of second-quarter results but there is trouble ahead. The question is, which will give way first -- fuel costs or passenger patience with an increasingly creaky system?
Amniotic membrane‐derived cells inhibit proliferation of cancer cell lines by inducing cell cycle arrest (2012)

Cells derived from the amniotic foetal membrane of human term placenta have drawn particular attention mainly for their plasticity and immunological properties, which render them interesting for stem-cell research and cell-based therapeutic applications. In particular, we have previously demonstrated that amniotic mesenchymal tissue cells (AMTC) inhibit lymphocyte proliferation in vitro and suppress the generation and maturation of monocyte-derived dendritic cells. Here, we show that AMTC also significantly reduce the proliferation of cancer cell lines of haematopoietic and non-haematopoietic origin, in both cell-cell contact and transwell co-cultures, therefore suggesting the involvement of yet-unknown inhibitory soluble factor(s) in this 'cell growth restraint'. Importantly, we provide evidence that the anti-proliferative effect of AMTC is associated with induction of cell cycle arrest in G0/G1 phase. Gene expression analyses demonstrate that AMTC can down-regulate cancer cells' mRNA expression of genes associated with cell cycle progression, such as cyclins (cyclin D2, cyclin E1, cyclin H) and cyclin-dependent kinase (CDK4, CDK6 and CDK2), whilst they up-regulate cell cycle negative regulator such as p15 and p21, consistent with a block in G0/G1 phase with no progression to S phase. Taken together, these findings warrant further studies to investigate the applicability of these cells for controlling cancer cell proliferation in vivo.
Algorithm for blind navigation along a GPS track (2011)

Most of existing navigation systems for the blind require a precise GPS maps. This makes them unusable in regions where there are no GPS maps, or they are not sufficiently accurate. Algorithm for GPS navigation for the visually impaired along a GPS track, which describes the path as a sequence of waypoints, is proposed. The main advantages of the algorithm are: natural voice navigation, adaptive to the velocity and accuracy of the GPS data, start of the navigation from any waypoint; correction of the direction of movement if it is necessary, return the user to the route if deviation is detected, work with and without electronic compass, detection of the movement of the user in the opposite direction.
Poly(diphenylacetylene) Membranes with High Gas Permeability and Remarkable Chiral Memory (2002)

ADVERTISEMENT RETURN TO ISSUEPREVCommunication to the...Communication to the EditorNEXTPoly(diphenylacetylene) Membranes with High Gas Permeability and Remarkable Chiral MemoryMasahiro Teraguchi and Toshio MasudaView Author Information Department of Polymer Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 606-8501, Japan Cite this: Macromolecules 2002, 35, 4, 1149–1151Publication Date (Web):January 17, 2002Publication History Received28 August 2001Revised30 November 2001Published online17 January 2002Published inissue 1 February 2002https://pubs.acs.org/doi/10.1021/ma011537fhttps://doi.org/10.1021/ma011537frapid-communicationACS PublicationsCopyright © 2002 American Chemical SocietyRequest reuse permissionsArticle Views1188Altmetric-Citations106LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Infrared light,Membranes,Permeability,Polymers,Solvents Get e-Alerts
Continuous dynamic flow micropumps for microfluid manipulation (2007)

Recent years have seen considerable progress in the development of microfabricated systems for use in the chemical and biological sciences. The term micro total analytical system (µTAS) is now a well-accepted concept. Much development has been driven by a need to perform effective manipulation of chemical and biological liquids with small volumes at micro and/or nano flowrate level in these systems. In this review, the focus will be on the pumping techniques used for delivery and control of liquids, especially those physical-chemical 'continuous dynamic flow micropumps'. The principles of these pumping techniques are mainly based on one or several well-known phenomena such as electrical, light, magnetic, thermal and other actuated mechanisms. Electrokinetically-driven continuous flow pumps such as the electrophoretic pump and electroosmotic pump, surface chemistry based continuous flow micropumps such as the opto-electrowetting-based pump, optically-driven pump, electrochemical pump and constant gravity-driven pump, and combination-driven techniques such as hydrodynamic flow and electrokinetic/gravity/magnetophoretic pumping will be summarized. The focus will be on the research highlights, trends and future of these pump techniques. Finally, mixing techniques on the microscale are briefly reviewed.
ISFET's using inorganic gate thin films (1979)

The characteristics of various types of ISFET's using inorganic gate films are described. The pH and pNa selectivities are investigated for SiO <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</inf> , Si <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</inf> N <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">4</inf> , Al <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</inf> O <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</inf> , alumino-silicate, and sodium-aluminosilicate gate dielectrics. The transient response and device stability are also studied for different values of solution pH. The Al <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</inf> O <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</inf> gate shows a nearly ideal pH response, excellent stability, and selectivity to other cations. On the other hand, the Si <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</inf> N <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">4</inf> gate is also a good pH sensor, but it is proved by the studies of SiO <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</inf> and SiO <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">x</inf> N <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">y</inf> films that the oxygen content in its surface degrades its properties as a pH sensor. Sodium-alumino-silicate, which is generally known as a material for pNa selective glass electrodes, is utilized as a gate film for the pNa ISFET. The pNa selectivity of this device is comparable to that of the conventional glass electrode. The alumino-silicate gate has also a pNa selectivity, but it is inferior to the sodium-alumino-silicate gate.
Study of Magnetic and Electric Coupling for Contactless Power Transfer Using Equivalent Circuits -Wireless Power Transfer via Electromagnetic Coupling at Resonance- (2010)

This paper proposes a novel method of contactless power transfer from a transmitting antenna to a receiving antenna. This method involves electromagnetic coupling of near-field antennas at resonance. However, the exact manner in which method this method can be used to design antennas and circuits has not been clarified. Therefore, this study focuses on defining the exact frequency and efficiency required fore electromagnetic coupling on the basis of the antenna theory, circuit theory, experimental observations, and electromagnetic computations and experiments.
Zur Absorption tiefer Frequenzen in Tonstudios (1992)

S.1-11
Fatty oils from oilseeds of forest origin as antibacterial agents. (1987)

Fatty oils extracted from seed of Cassia tora Linn., Wrightia tomentosa Roem &Schult., Viburnum coriaceum Blume, Michelia champaca Linn., Pongamia pinnato and Azadirachta indica A Juss., have been tested in vitro against five species of bacteria i.e. Bacillus pumilus Gottheil, Bacillus subtilis Cohn, Salmonella typhosa (Zopf) white syn. Salmonella typhi Warren and Scott., S paratyphi (Kayer) Casteliani and Chalmers and Micrococcus pyogens var. albus (Rosenbach, Schroeten) Syn. Staphylococcus albus Rosenbach. Present investigations reveal that all the above mentioned fatty oils have potent antibacterial efficay against Bacillus subtilis, Salmonella typhosa and S paratyphi .
Temporal Database Architecture Enhancements (2018)

Database systems used in the past were characterized by storing only current valid states., which is not., however., optimal for intelligent systems and applications. Temporal paradigm allows us to delimit the object state with the time validity regarding the modeled granularity. Temporal systems are based on the extension of conventional approaches., which do not provide powerful solutions. Database server architecture is described with emphasis on the optimization options to improve and shorten the data retrieval process. In this paper., we attach significance to the migrated row., which forces the system to load multiple data blocks from the database into the memory. The solution., based on the evolution steps., is implemented using the mapping module inside the memory of the database instance.
Monte Carlo model of the scanning beam digital x-ray (SBDX) source (2012)

The scanning-beam digital x-ray (SBDX) system has been developed for fluoroscopic imaging using an inverse x-ray imaging geometry. The SBDX system consists of a large-area x-ray source with a multihole collimator and a small detector. The goal of this study was to build a Monte Carlo (MC) model of the SBDX source as a useful tool for optimization of the SBDX imaging system in terms of its hardware components and imaging parameters. The MC model of the source was built in the EGSnrc/BEAMnrc code and validated using the DOSXYZnrc code and Gafchromic film measurements for 80, 100, and 120 kV x-ray source voltages. The MC simulated depth dose curves agreed with measurements to within 5%, and beam profiles at three selected depths generally agreed within 5%. Exposure rates and half-value layers for three voltages were also calculated from the MC simulations. Patient skin-dose per unit detector-dose was quantified as a function of patient size for all three x-ray source voltages. The skin-dose to detector-dose ratio ranged from 5-10 for a 20 cm thick patient to 1 × 10(3)-1 × 10(5) for a 50 cm patient for the 120 and 80 kV beams, respectively. Simulations of imaging dose for a prostate patient using common imaging parameters revealed that skin-dose per frame was as low as 0.2 mGy.
Sonas Concepts, Architecture, and Planning Guide (2012)

IBM Scale Out Network Attached Storage (IBM SONAS) is a Scale Out NAS offering designed to manage vast repositories of information in enterprise environments requiring very large capacities, high levels of performance, and high availability.SONAS provides a range of reliable, scalable storage solutions for a variety of storage requirements. These capabilities are achieved by using network access protocols such as NFS, CIFS, HTTP, and FTP.Using built-in RAID technologies, all data is well protected with options to add additional protection through mirroring, replication, snapshots, and backup. These storage systems are also characterized by simple management interfaces that make installation, administration, and troubleshooting uncomplicated and straightforward.This book is the companion to the IBM Redbooks publication, SONAS Implementation and Best Practices Guide, SG24-7962. In this IBM Redbooks publication, we discuss the marketplace requirements that led to the SONAS stand-alone storage system. We introduce storage architectures and file systems. We then introduce the reader to SONAS and describe the hardware and software that make up the SONAS appliance. This book provides the planning information needed to configure the SONAS order based on your enterprise requirements.
Fruit‐specific overexpression of wound‐induced <i>tap1</i> under E8 promoter in tomato confers resistance to fungal pathogens at ripening stage (2012)

Based on high economic importance and nutritious value of tomato fruits and as previous studies employed E8 promoter in fruit ripening-specific gene expression, we have developed transgenic tomato plants overexpressing tomato anionic peroxidase cDNA (tap1) under E8 promoter. Stable transgene integration was confirmed by polymerase chain reaction (PCR) and Southern analysis for nptII. Northern blotting confirmed elevated tap1 levels in the breaker- and red-ripe stages of T(1) transgenic fruits, whereas wild-type (WT) plants did not show tap1 expression in these developmental stages. Further, tap1 expression levels were significantly enhanced in response to wounding in breaker- and red-ripe stages of transgenic fruits, whereas wound-induced expression of tap1 was not detected in WT fruits. Confocal microscopy revealed high accumulation of phenolic compounds at the wound site in transgenic fruits suggesting a role of tap1 in wound-induced phenolic polymerization. Total peroxidase activity has increased remarkably in transgenic pericarp tissues in response to wounding, while very less or minimal levels were recorded in WT pericarp tissues. Transgenic fruits also displayed reduced post-harvest decay and increased resistance toward Alternaria alternata and Fusarium solani infection with noticeable inhibition in lesion formation. Conidiospore germination and mycelial growth of F. solani were severely inhibited when treated with E8-tap1 fruit extracts compared to WT fruits. 3-(4,5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay showed reduced spore viability when incubated in E8-tap1 fruit extracts. Thus, fruit-specific expression of tap1 using E8 promoter is associated with enhanced total peroxidase activity and high phenolic accumulation in fruits with minimized post-harvest deterioration caused by wounding and fungal attack in tomato fruits.
Discovering de novo peptide substrates for enzymes using machine learning (2018)

The discovery of peptide substrates for enzymes with exclusive, selective activities is a central goal in chemical biology. In this paper, we develop a hybrid computational and biochemical method to rapidly optimize peptides for specific, orthogonal biochemical functions. The method is an iterative machine learning process by which experimental data is deposited into a mathematical algorithm that selects potential peptide substrates to be tested experimentally. Once tested, the algorithm uses the experimental data to refine future selections. This process is repeated until a suitable set of de novo peptide substrates are discovered. We employed this technology to discover orthogonal peptide substrates for 4'-phosphopantetheinyl transferase, an enzyme class that covalently modifies proteins. In this manner, we have demonstrated that machine learning can be leveraged to guide peptide optimization for specific biochemical functions not immediately accessible by biological screening techniques, such as phage display and random mutagenesis.
Extrusion of CP Grade Titanium Powders Eliminating the Need for Hot Pre-Compaction via Hot Isostatic Pressing (2007)

Extrusion is a way to produce near net shape components from CP grade titanium powders of optimum density with minimum porosity and acceptable mechanical properties. Chemically pure, hydride/dehydride titanium powders were cold pre-compacted and extruded at 850oC under an argon atmosphere. The extrusion stress required was ~450MPa. To characterize the extrusions, the porosity distribution, qualitative microstructure and tensile properties were evaluated and compared with conventional extruded wrought titanium. Extrusion occurred after the green billets were upset to ~100% of theoretical density and adequate lubrication was applied to the die. The resultant product was 100% dense with a narrow band of surface porosity and exhibited an equiaxed microstructure of similar magnitude to the starting material. The tensile properties of the bars were observed to be significantly superior to conventionally extruded CP titanium bar products, a result associated with the much finer average grain size. Outcomes from this study have assisted in the identification of a number of key characteristics important to the extrusion of titanium from pre-compacted CP titanium powders, allowing the elimination of canning and hot isostatic pressing (HIPping) of billets prior to extrusion as per conventional PM processes.
Cancer-associated isoenzyme of serum galactosyltransferase. (1976)

Galactosyltransferase activity was assayed in sera from 58 patients with various types of cancer. On discontinuous polyacrylamide gel electrophoresis a slow-moving peak of galactosyltransferase activity (isoenzyme II) was found to be present in the serum of 43 of these patients in addition to the major isoenzyme I. Isoenzyme II was found in only 2 of 39 patients with various nonmalignant disorders and was not detected in the serum of 22 normal control subjects. There was no correlation between the presence of this electrophoretically distinct isoenzyme and total serum galactosyltransferase activity, alkaline phosphatase, levels of carcinoembryonic antigen, or blood type. However, patients with widespread metastases had significantly higher isoenzyme II levels than those with no metastases or with limited local spread. Further studies will be necessary to evaluate the clinical usefulness of this serum galactosyltransferase isoenzyme in the diagnosis and monitoring of patients with neoplastic disease.
Biological activity and complementation of the two peptides of staphylococcal enterotoxin B formed by limited tryptic hydrolysis. (1975)

Tryptic hydrolysis after reduction and carboxamidomethylation of staphylococcal enterotoxin B cleaved the single susceptible bond located between the 2 half-cystines of the molecule, Lys-Thr at positions 97 and 98 (Spero, L., Warren, J. R., and Metzger, J. F. (1973) J. Biol. Chem. 248, 7289-7294). The product remained as a single particle but was separated into the two constituent peptides by denaturants, and purification of the two fragments was accomplished by chromatography on CM-cellulose in 8 M urea. Antigenic activity was exhibited by the separated peptides after dialysis of urea solutions against dilute buffer, but was very labile. No emetic activity in rhesus monkeys was found for either separated peptide. The derivative behaved as two random coil peptides in 6 M guanidine hydrochloride but upon removal of guanidine refolded to a single molecular entity. Viscosity and unfolding kinetics in 1.5 M guanidine indicated physical identity of the recombined peptides with the derivative prior to treatment with guanidine. Three biological measures (serological, mitogenic, and emetic activity) were also essentially unaltered for the recombined material. Since these biological activities are dependent upon different aspects of enterotoxin structure, it is concluded that the recombined derivative was restored to its original conformation.