gebhart/patrepeval huggingface dataset:
Effects of genetic background on prostate and taste bud carcinogenesis due to SV40 T antigen expression under probasin gene promoter control (2002)

The incidence of prostate carcinomas in African-American men is greater than in white men, indicating genetic factors are involved in risk of this neoplasia. Recently, we have developed a transgenic rat model of prostate cancer, featuring development of malignancies within 15 weeks of age at very high incidence. Male transgenic rats with a Sprague-Dawley genetic background were mated with wild-type females of F344, Wistar and ACI strains. F1 male transgenic hybrids with female Wistar and ACI rats had significantly lowered incidences of prostate carcinomas. However, the serum level of testosterone, and expression of the transgene, probasin, and the androgen receptor did not correlate with the strain variation in tumor development. Furthermore, immunohistochemical analysis of the SV40 Tag and the androgen receptor also did not reveal any differences between the strains. The transgenic rats additionally developed taste bud neuroblastomas at 100% incidence and this was suppressed in F1 male transgenic offspring with the ACI, but not the other strains. These results clearly show that genetic background influences prostate carcinogenesis and taste bud tumorigenesis in rats and that the present transgenic rats could provide a good model to identify specific factors.
Transcriptional Analysis of Different Promoters in the <i>sar</i> Locus in <i>Staphylococcus aureus</i> (1998)

The expression of extracellular virulence determinants in Staphylococcus aureus is controlled by a 510-nucleotide RNA molecule (RNAIII) which is a part of the agr system. The agr operon, which encodes a multicomponent signal transduction system, is partially under the influence of an unlinked regulatory locus called sar. The sar locus is composed of three overlapping transcripts, designated sarA (0.56 kb), sarC (0.8 kb), and sarB (1.2 kb), originating from the P1, P3, and P2 promoters, respectively. In this study, we analyzed the differential expression of these promoters by using transcriptional fusion with the xylE reporter gene to study the activation of the sar locus. The data confirm the existence of three independent promoters with different promoter activities. Maximal promoter activity was observed with the combined fusion of P2-P3-P1 promoters. Expression studies with a sigB mutant revealed that the P3 promoter is SigB dependent. Analysis of these transcriptional fusions in a sarA mutant and in complemented strains with each of the sar transcriptional units revealed that the sar locus is autoregulatory, with SarA acting as a positive regulator. From various transcriptional fusion studies of the upstream region of the P1 promoter, we have localized a 34-bp sequence which seems to play a role in down-modulating P1 transcription. Using heparin-Sepharose and DNA-specific columns, we partially purified a 12-kDa protein, possibly a repressor, which binds to the promoter regions upstream of P2 and P1 and which also binds to the 34-bp sequence. These data indicated that the regulation of the sar locus is complex and may involve the sar gene product(s) and other regulatory protein(s).
Carbon Nanotubes Modified Solid Electrolyte-Based Structural Supercapacitors and their Temperature Influence (2017)

In the present work, we reporton structural supercapacitors which are based on NASICON-type solid electrolyte Li1.4Al0.4Ti1.6(PO4)3(LATP). The nanostructuredelectrodes incorporate single-wall carbon nanotubes (SWCNTs) mixedwith the LATP electrolyte. The complete energy storage devices are manufactured ina sandwich structure consisting of two nanostructured electrode layers which are separated by a pure LATP layer.The as-prepared specimens are embedded in composite materials with Airstone880/886H epoxy resin as matrix. Theirelectrical properties are characterized by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). Atambient temperature, the addition of 6.5 wt.% SWCNTs results in a distinct improvementby reducingthe total resistance of the embedded devices and enhancesthe capacitance from 0.025 mFg-1to 3.160 mFg-1at a scan rate of 5 mVs-1. Electrical measurements of two types of specimens are then applied underdifferent temperatures from ambient temperatureto 80 °C. It is observed that theequivalentseriesresistance (ESR) of device with SWCNTs decreases greatly and capacitanceincreases comparing with the device without SWCNTs. As a conclusion, the structural supercapacitors acquire excellent performance through high efficient double layer effectsrealized by nanostructured electrode/electrolyte interphase (large surface electrode areas).
Remote Password Extraction from RFID Tags (2007)

Side-channel attacks are used by cryptanalysts to compromise the implementation of secure systems. One very powerful class of side-channel attacks is power analysis, which tries to extract cryptographic keys and passwords by examining the power consumption of a device. We examine the applicability of this threat to electromagnetically coupled RFID tags. Compared to standard power analysis attacks, our attack is unique in that it requires no physical contact with the device under attack. Power analysis can be carried out even if both the tag and the attacker are passive and transmit no data, making the attack very hard to detect. As a proof of concept, we describe a password extraction attack on Class 1 Generation 1 EPC tags. We also show how the privacy of Class 1 Generation 2 tags can be compromised by this attack. Finally, we examine possible modifications to the tag and its RF front end which help protect against power analysis attacks.
STATE-OF-THE-ART AND FUTURE DEVELOPMENTS IN INTEGRATED CHASSIS CONTROL FOR GROUND VEHICLES (2015)

<p>Many modern ground vehicles feature state-of-the-art powertrain, braking and suspension control systems. These technologies are rapidly filtering through to heavy and off-road vehicles. The drawback of many of these systems is that their operation is largely considered only in stand-alone mode. The paper introduces up-to-date and coming ground vehicle technology related to the integration and advanced control of active chassis control systems. In particular, addressing the task of coordinated subsystems control can provide simultaneous enhancements in traction and braking performance, handling, off-road mobility, driving comfort and energy efficiency. A special focus in the paper is given to coordinated operation of brake control, active suspension, and dynamic tyre pressure management. The influence of each particular subsystem on the vehicle safety, off-road mobility and ride comfort is evaluated in simulation. It is further described and confirmed in simulation how the integrated chassis control (ICC) can simultaneously improve each of these vehicle characteristics. From the hardware viewpoint, a variant of ground vehicle architecture with aforementioned integrated active chassis systems is introduced. This architecture and its corresponding implementation on a sport utility vehicle are currently investigated within the European Union-funded Horizon 2020 consortium EVE. The work presented is a collaborative effort among several ISTVS members across the globe.</p>
A Torque-Sensitive Tactile Array for Robotics (1983)

We introduce a new tactile sensor and show its advantages for robotic applications. The tactile array elements are composed of magnetic dipoles (in an elastic medium) whose position and orientation are detected by magnetoresistive sensors. We show that, unlike the existing tactile sensor designs, this device is sensitive to torque as well as to normal and tangential forces. We demonstrate experimentally the fabrication, sensitivity, and repeatability of the tactile elements.
Conditional Split Lattice Vector Quantization For Spectral Encoding Of Audio Signals (2006)

Publication in the conference proceedings of EUSIPCO, Florence, Italy, 2006
Evaluation of Engineered CRISPR-Cas-Mediated Systems for Site-Specific RNA Editing (2020)

Site-directed RNA editing approaches offer great potential to correct genetic mutations in somatic cells while avoiding permanent off-target genomic edits. Nuclease-dead RNA-targeting CRISPR-Cas systems recruit functional effectors to RNA molecules in a programmable fashion. Here, we demonstrate a Streptococcus pyogenes Cas9-ADAR2 fusion system that uses a 3' modified guide RNA (gRNA) to enable adenosine-to-inosine (A-to-I) editing of specific bases on reporter and endogenously expressed mRNAs. Due to the sufficient nature of the 3' gRNA extension sequence, we observe that Cas9 gRNA spacer sequences are dispensable for directed RNA editing, revealing that Cas9 can act as an RNA-aptamer-binding protein. We demonstrate that Cas9-based A-to-I editing is comparable in on-target efficiency and off-target specificity with Cas13 RNA editing versions. This study provides a systematic benchmarking of RNA-targeting CRISPR-Cas designs for reversible nucleotide-level conversion at the transcriptome level.
Clonal variation of human induced pluripotent stem cells for induction into the germ cell fate† (2017)

The mechanisms for human germ cell development have remained largely unknown, due to the difficulty in obtaining suitable experimental materials. The establishment of an in vitro system to reconstitute human germ cell development will thus provide a critical opportunity to understand its mechanisms at a molecular level. It has previously been shown that human induced pluripotent stem cells (hiPSCs) are first induced into incipient mesoderm-like cells (iMeLCs), which are in turn induced into primordial germ-cell like cells (PGCLCs) with gene expression properties similar to early migratory PGCs. Here, we report that the efficiency of PGCLC induction varies among hiPSC clones, and, interestingly, the clonal variations in PGCLC induction efficiency are reflected in the gene expression states of the iMeLCs. Remarkably, the expression levels of EOMES, MIXL1, or T in the iMeLCs are positively correlated with the efficiency of subsequent PGCLC generation, while the expressions of CDH1, SOX3, or FGF2 are negatively correlated. These results indicate that the expression changes of these genes occurring during iMeLC induction are key markers indicative of successful induction of PGCLCs, and furthermore, that hiPSC clones have different properties that influence their responsivity to the iMeLC induction. Our study thus provides important insights into the mechanism of hPGC specification as well as the development of a better strategy for inducing human germ cell fate from PSCs in vitro.
ChemInform Abstract: Band Excitation in Scanning Probe Microscopy: Recognition and Functional Imaging (2014)

Abstract Review: 97 refs.
Trapping of individual airborne absorbing particles using a counterflow nozzle and photophoretic trap for continuous sampling and analysis (2014)

We describe an integrated opto-aerodynamic system and demonstrate that it enables us to trap absorbing airborne micron-size particles from air, hold them and then release them, and to repeat this sequence many times as would be appropriate for continuous sampling of particles from air. The key parts of the system are a conical photophoretic optical trap and a counter-flow coaxial-double-nozzle that concentrates and then slows particles for trapping. This technology should be useful for on-line applications that require monitoring (by single particle analyses) of a series of successively arriving particles (e.g., from the atmosphere or pharmaceutical or other production facilities) where the total sampling time may last from minutes to days, but where each particle must be held for a short time for measurements (e.g., Raman scattering).
Effects of selective hypoglossal nerve stimulation on canine upper airway mechanics (2005)

Electrical stimulation of the hypoglossal (XII) nerve has been demonstrated as an effective approach to treating obstructive sleep apnea. The physiological effects of conventional modes of stimulation (i.e., genioglossus activation or whole XII nerve stimulation), however, have yielded inconsistent and only partial alleviations of hypopneic or apneic events. Although selective stimulation of the multifasciculated XII nerve offers many stimulus options, it is not clear how these will functionally affect the upper airway (UAW). To study these effects, animal experiments in eight beagles were performed to investigate changes in the UAW resistance and critical pressure during simulated expiration (n = 4) and inspiration (n = 4). During expiration, nonselective XII nerve stimulation yielded the greatest improvement in UAW resistance (-0.66 +/- 0.11 cm H2O x l(-1) x min(-1)), compared with that for selective activation of the geniohyoid (-0.29 +/- 0.09 cm H2O x l(-1) x min(-1)), genioglossus (-0.31 +/- 0.12 cm H2O x l(-1) x min(-1)), and hyoglossus/styloglossus (0.37 +/- 0.06 cm H2O x l(-1) x min(-1)) muscles. For simulated inspiration, on the other hand, only whole XII nerve stimulation (-0.9 +/- 0.4 cm H2O) and coactivation of the genioglossus + hyoglossus/styloglossus muscles (-1.18 +/- 0.6 cm H2O) produced significant (P < 0.05) improvements in UAW stability (i.e., lowered critical pressure), compared with baseline (-0.52 +/- 0.32 cm H2O). The results of this study suggest that a multicontact nerve electrode can be used to achieve both UAW dilation and patency, comparable to that obtained with nonselective stimulation, by selectively activating the various branches of the XII nerve.
Construction of Heat-Insulated Engine with Large Heat-Insulation Rate. (1993)

In order to eliminate the cooling system from all internal combustion engine, we studied heat-insulated construction of an engine. It is very difficult to increase the heat-insulation rate to above 50% in a heat-insulated engine having a combustion chamber wall coated with a zirconia layer. Heat flux and temperatures have been calculated by means of the finite-element method. Calculated results are compared with the measured temperatures in an engine which has a combustion chamber wall made of silicon nitride and a compound heat-insulated construction composed of an air gap and a gasket with very low thermal conductivity. We obtained the results that the compound heat-insulated construction is very effective in reducing heat flow from the combustion chamber wall to the outer cylinder made of cast iron. The calculated results show good correspondence with the measured data when large heat-transfer coefficients are used for the piston when fuel is burning briskly, and when low heat-transfer coefficients are employed on the cylinder liner in contact with the combustion gas.
Applying PII fingerprints in security incident analysis (2012)

Regulations in many countries govern the use of personally identifiable information (PII) in IT systems. A key aspect of these regulations is to retain PII only as long as necessary and delete it immediately afterwards. Organizations should also consider retaining PII only for the minimum period as business requirements demand it for liability reasons. A difficult sit-uation arises for an organization if the possibility of a compromise of PII is detected after the PII has been deleted. Today, in such a situation, the scope of the potential compromise cannot easily be ascertained. Furthermore, the owner of the PII cannot easily be informed. We propose a novel algorithm to generate PII fingerprints which allows the determination of the scope of the affected PII in case a compromise is confirmed. The benefit is the ability to determine the exact scope of a potential compromise.
Prostate Specific Antigen in Benign Prostatic Hyperplasia: Purification and Characterization (1997)

Most PSA in BPH nodules is in the nicked form with low chymotrypsin-like activity. When it leaks into the circulation it will form fewer PSA-antichymotrypsin complexes and more will remain in the free form. We predict that a protease with trypsin-like activity in BPH nodule fluid is probably responsible for the nicked form of BPH PSA. These findings suggest that antibodies produced against PSA in BPH nodules may be useful in discriminating prostate cancer from BPH.
Design, Synthesis, and Biological Activity of 1,2,3-Triazolobenzodiazepine BET Bromodomain Inhibitors (2017)

A number of diazepines are known to inhibit bromo- and extra-terminal domain (BET) proteins. Their BET inhibitory activity derives from the fusion of an acetyl-lysine mimetic heterocycle onto the diazepine framework. Herein we describe a straightforward, modular synthesis of novel 1,2,3-triazolobenzodiazepines and show that the 1,2,3-triazole acts as an effective acetyl-lysine mimetic heterocycle. Structure-based optimization of this series of compounds led to the development of potent BET bromodomain inhibitors with excellent activity against leukemic cells, concomitant with a reduction in c-<i>MYC</i> expression. These novel benzodiazepines therefore represent a promising class of therapeutic BET inhibitors.
Deep Reinforcement Learning-Based Image Captioning with Embedding Reward (2017)

Image captioning is a challenging problem owing to the complexity in understanding the image content and diverse ways of describing it in natural language. Recent advances in deep neural networks have substantially improved the performance of this task. Most state-of-the-art approaches follow an encoder-decoder framework, which generates captions using a sequential recurrent prediction model. However, in this paper, we introduce a novel decision-making framework for image captioning. We utilize a policy network and a value network to collaboratively generate captions. The policy network serves as a local guidance by providing the confidence of predicting the next word according to the current state. Additionally, the value network serves as a global and lookahead guidance by evaluating all possible extensions of the current state. In essence, it adjusts the goal of predicting the correct words towards the goal of generating captions similar to the ground truth captions. We train both networks using an actor-critic reinforcement learning model, with a novel reward defined by visual-semantic embedding. Extensive experiments and analyses on the Microsoft COCO dataset show that the proposed framework outperforms state-of-the-art approaches across different evaluation metrics.
DNA double strand breaks in epidermal cells cause immune suppression in vivo and cytokine production in vitro (1996)

UV irradiation of the skin causes immune suppression by a mechanism involving epidermal cytokines. To determine the role of epidermal DNA damage in immune suppression, we used HindIII restriction endonuclease encapsulated in liposomes to cause DNA strand breaks in epidermal cells in vivo and in vitro. Topical application of HindIII in liposomes to murine skin in vivo impaired the induction of contact hypersensitivity responses initiated either locally or at distant sites and impaired the function of APCs. Unlike UV-B radiation, however, treatment of mice with HindIII in liposomes before contact sensitization did not induce tolerance or transferable suppression. The liposome-encapsulated HindIII caused double strand breaks in DNA and induced IL-10 and TNF-alpha production when added to cells of a murine keratinocyte line in vitro. Topical application of liposomal HindIII also induced TNF-alpha in the epidermis of mice. Liposomes containing heat-inactivated HindIII or an endonuclease specific for pyrimidine dimers in DNA did not exhibit these effects. These results support the hypothesis that DNA damage is a trigger for the production of cytokines that modulate immune responses. They also suggest that immune suppression and suppressor cell induction are separate consequences of cutaneous injury that require different stimuli.
Candidate odorant receptors from the malaria vector mosquito <i>Anopheles gambiae</i> and evidence of down-regulation in response to blood feeding (2001)

Olfaction plays a major role in host preference and blood feeding, integral behaviors for disease transmission by the malaria vector mosquito Anopheles gambiae sensu stricto (henceforth A. gambiae). We have identified four genes encoding candidate odorant receptors from A. gambiae that are selectively expressed in olfactory organs, contain approximately seven transmembrane domains, and show significant similarity to several putative odorant receptors in Drosophila melanogaster. Furthermore, one of the putative A. gambiae odorant receptors exhibits female-specific antennal expression and is down-regulated 12 h after blood feeding, a period during which substantial reduction in olfactory responses to human odorants has been observed. Taken together, these data suggest these genes encode a family of odorant receptors in A. gambiae, whose further study may aid in the design of novel antimalarial programs.
Antenna design for wireless electrocorticography (2012)

We present a design methodology for implantable antennas patterned on a flexible substrate for electrocorticography. The antennas are designed for maximum link gain across the human skull to efficiently power the implant. Simulation results show that optimizing the transmission frequency together with antenna geometries can provide sufficient power and voltage to the implant.
IL-2 and IL-4 Double Knockout Mice Reject Islet Allografts: A Role for Novel T Cell Growth Factors in Allograft Rejection (1998)

T cell growth factors (TCGFs) play a critical role in allograft rejection by promoting the activation and proliferation of alloreactive T cells. To determine whether IL-2 and IL-4 are of quintessential importance in allograft rejection and to identify possible alternative TCGFs, we have bred IL-2(-/-) and IL-4(-/-) double knockout (DKO) mice and studied islet allograft rejection using the DKO mice as allograft recipients. Although mononuclear leukocytes from DKO mice did not mount a proliferative response in vitro in response to anti-CD3 stimulation, crude islet allografts were vigorously rejected by DKO mice (mean survival time 17 +/- 7, n = 8) as compared with wild-type controls (mean survival time 13 +/- 4, n = 7). Treatment of DKO mice with anti-CD3 or rapamycin markedly prolonged the islet allograft survival. An analysis of intragraft cytokine gene transcripts showed robust expression of IL-7 and IL-15. In contrast, intragraft IL-9 gene transcripts were not detected in either wild-type or DKO mice. Provision of exogenous IL-2, IL-4, IL-7, or IL-15, but not IL-9, supports the proliferation of anti-CD3 activated DKO splenic leukocytes in vitro. Blocking the common gamma c of IL-2 receptor, a shared essential signaling component by receptors for IL-2, IL-4, IL-7, IL-9, and IL-15, prolonged the survival of islet allografts in DKO mice. Hence, a T cell dependent allograft rejection enabled by rapamycin-sensitive signals or signals mediated by binding of the gamma c chain occurs in the absence of both IL-2 and IL-4. Non-T cell-derived TCGFs, especially IL-7 and IL-15, may play an active role in supporting allograft rejection.
Balanced control strategies for interconnected heterogeneous battery systems (2016)

This paper develops new balanced charge/discharge strategies that distribute charge or discharge currents properly so that during operation battery systems maintain uniform state-of-charge (SOC) all the time. The proposed balanced charge/discharge control strategies are useful for interconnected heterogeneous battery systems that can be built from battery modules with different types, ages, and power/capacity ratings. Both voltage-based and SOC-based balanced charge/discharge strategies are developed. Their convergence properties are rigorously established, and illustrative examples using production batteries demonstrate their convergence behavior under different charging current profiles. The approach will be especially useful for battery storage systems to support power grids with renewable energy sources where the battery systems are required to operate continuously.