gebhart/patrepeval huggingface dataset:
Mutants of Saccharomyces cerevisiae defective in the farnesylation of Ras proteins. (1990)

Ras proteins are post-translationally modified by farnesylation. In the present investigation, we identified an activity in crude soluble extracts of yeast cells that catalyzes the transfer of a farnesyl moiety from farnesyl pyrophosphate to yeast RAS2 protein. RAS2 proteins having a C-terminal Cys-Ali-Ali-Xaa sequence (where Ali is an aliphatic amino acid and Xaa is the unspecified C-terminal amino acid) served as substrates for this reaction, whereas RAS2 proteins with an altered or deleted Cys-Ali-Ali-Xaa sequence did not. A yeast mutant, dpr1/ram1, originally isolated as a Ras-processing mutant was shown to be defective in farnesyltransferase activity. In addition, another mutant, ram2, also was defective in the transferase activity. These results demonstrate that at least two genes, DPR1/RAM1 and RAM2, are required for the farnesyltransferase activity in yeast.
Ring‐opening metathesis polymerization of a modified linseed oil with varying levels of crosslinking (2008)

Abstract A commercial norbornyl‐functionalized linseed oil, blended with a bicyclic norbornene‐based crosslinking agent (at loadings ranging from 0 to 50 wt %) undergoes ring‐opening metathesis polymerization with the 1st generation Grubbs' catalyst to form a biorenewable polymer network. Comonomers are characterized, the thermal and mechanical properties of the cured systems are investigated by dynamic mechanical analysis, and thermal decomposition is evaluated by thermogravimetric analysis. The resin is shown to consist of a modified linseed oil and small oligomers of cyclopentadiene. Broad tan δ peaks suggest inhomogeneous phase morphologies, which result in complex crosslinking behaviors. The thermal stability of the polymers increases with increasing crosslinker content. © 2008 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 46: 6851–6860, 2008
Identification of Real-Time Diagnostic Measures of Visual Distraction With an Automatic Eye-Tracking System (2006)

Potential applications of this research include assessing visual distraction in real time, delivering advisories to distracted drivers to reorient their attention to driving, and using distraction information to adapt forward collision and lane departure warning systems to enhance system effectiveness.
A comparison of the antiserotonin, antihistamine, and anticholinergic activity of cyproheptadine with analogs having furan nuclei fused to the 10,11-vinylene bridge (1977)

From the archives of Untitled, Radio, this episode looks back on four years of contributors to Untitled radio who have the double-X chromosome and a triple-X threat. Contributors include powerful women in the contemporary art world including Liliana Porter, Sylvia Meyer, INVASORIX, Cathy Byrd with Zoe Buckman, Anna Ostoya and Ben Lerner, artist collective CARNE, Catherine Taft with Badlands Unlimited, FLUCT, as well as Autumn Knight and Chelsea Knight.\n
Three-Axis Nested Fiber Optic Gyroscope (1999)

This paper provides a description of the Three-Axis
Nested Fiber Optic Gyroscope (FOG). The Three-Axis
FOG is being developed by System Engineering
Solutions, Inc. (SES) and its prime subcontractor
Fibersense Technology Corp. (FTC) under sponsorship by
the Aviation Applied Technology Directorate of the US
Army Aviation and Missile Command, a Phase II SBIR.
The Three-Axis FOG being developed by SES is based on
the design of a nested configuration of three free standing
elliptical fiber optic coils, patent pending, in a supporting
assembly that yields substantial size and weight
advantages. It permits use of an integrated three axis
environmental shield and thermal isolation enclosure.
The core nest ed assembl y of three fr ee st andi ng coi l s
mounted in its supporting structure with electro-optical
components weighs less than 0.4 pounds and its volume is
19 cubic inches, not including shielding and isolation
enclosure featur es whi ch are appl i cati on and i nt er face
dependent.
The corresponding Performance Projections for the Army
Three-Axis Gyroscope with a 500 deg/sec rate range are,
uncompensated, Bias of 0.007 deg/hr, 1 sigma, Angle
Random Walk of 0.002 deg/rt-hr.
To date SES has completed the detailed design of the
nested configuration and the enclosure. The supporting
FTC digital control and interface electroni cs has been
adapt ed to the SES opt i cal packagi ng confi gu rati on.
Fabrication of two deliverable Nested Fiber Optic Gyro
Systems was initiated May 1999.
Ext ensi ve t est i ng of t he FTC electroni cs wi t h the Phase I
SBIR FOG has been conducted to verify compatibility
bet ween the FOG electro -opt i cal featu res and t he di gi t al
cont rol electroni cs. Speci fi c emphasi s was on t he
determination of thermal and voltage sensitivities. To
date in temperature tests, across 20 degC temperature
cycling, bias performance of 0.033 deg/hr has been
achieved with no temperature control or compensation
modeling. Angle Random Walk of 0.0038 deg/rt-hr has
also been demonst rated. The Phase II coi l has 23% more
coil length and unique thermal isolation and control is implemented. This enhanced design is expected to yield
the performance projections tabulated above.
Distinctive Features of Saccadic Intrusions and Microsaccades in Progressive Supranuclear Palsy (2011)

The eyes do not stay perfectly still during attempted fixation; fixational eye movements and saccadic intrusions (SIs) continuously change the position of gaze. The most common type of SI, square-wave jerk (SWJ), consists of pairs of horizontal saccades: the first saccade moves the eye away from the fixation target and, after a short interval, the second saccade brings it back towards the target. SWJs are prevalent in certain neurological disorders, including progressive supranuclear palsy (PSP). Here we developed an objective method to identify SWJs. We confirmed that SWJs are more frequent, larger and more markedly horizontal in PSP patients than in healthy human subjects. We also discovered that the loss of a vertical component in fixational saccades and SWJs is the eye movement feature that best distinguishes PSP patients from controls. We moreover found that in PSP patients and controls, the larger the saccade the more likely it is part of a SWJ. Further, all saccades produced by PSP patients had equivalent properties whether they were part of a SWJ or not, suggesting that normal fixational saccades (microsaccades) are rare in PSP. We propose that fixational saccades and SIs are generated by the same neural circuit, and that, both in PSP patients and in healthy subjects, SWJs result from a coupling mechanism that generates a second corrective saccade shortly after a large fixation saccade. Due to brainstem and/or cerebellum impairment, fixational saccades in PSP are abnormally large, and thus more likely to trigger a second corrective saccade, giving rise to SWJs.
Novel fabrication methods and characteristics of organic complementary circuits (2003)

The success of organic circuits depends on the implementation of novel fabrication techniques, and circuit designs which utilize organic semiconductor characteristics. We present DC, transient, and noise measurements of discrete organic FETs, and use these characteristics in SPICE simulations of complementary MOS circuits ranging in complexity from inverters through shift registers. Source/drain contacts are fabricated using electroless Ni/Au electrode deposition, a method well suited for incorporation with other fabrication techniques such as screen printing and microcontact printing.
Architectural Extensions for Elliptic Curve Cryptography over GF(2^m ) on 8-bit Microprocessors (2006)

We describe and analyze architectural extensions to accelerate the public key cryptosystem elliptic curve cryptography (ECC) on 8-bit microprocessors. We show that simple extensions of the data path suffice to efficiently support ECC over GF(2/sup m/). These extensions include an extended multiplier that generates results for both integer multiplications and multiplications in fields GF(2/sup m/) and a multiply-accumulate instruction for efficiently performing multiple precision multiplications. To our knowledge, this is the first paper that quantifies performance of standard NIST and SECG elliptic curves over GF(2/sup m/) on an 8-bit microprocessor equipped with a dual field multiplier. On the ATmegal28 microprocessor running at 8 MHz we measured an execution time of 0.29 s for a 163-bit ECC point multiplication over GF(2/sup m/), 0.81s for a 160-bit ECC point multiplication over GF(p), and 11 s for a 1024-bit RSA private key operation - the chosen key sizes provide equivalent security strength.
Signature of a pair of Majorana zero modes in superconducting gold surface states (2020)

Under certain conditions, a fermion in a superconductor can separate in space into two parts known as Majorana zero modes, which are immune to decoherence from local noise sources and are attractive building blocks for quantum computers. Promising experimental progress has been made to demonstrate Majorana zero modes in materials with strong spin-orbit coupling proximity coupled to superconductors. Here we report signatures of Majorana zero modes in a material platform utilizing the surface states of gold. Using scanning tunneling microscope to probe EuS islands grown on top of gold nanowires, we observe two well-separated zero-bias tunneling conductance peaks aligned along the direction of the applied magnetic field, as expected for a pair of Majorana zero modes. This platform has the advantage of having a robust energy scale and the possibility of realizing complex designs using lithographic methods.
Genes and Elite Athletes (2004)

The ability to become an elite athlete requires a rare convergence of both environmental and genetic factors. While we have known for several decades that genetic factors have a strong influence on athletic potential, we have only recently begun to identify the actual genes responsible. This article reviews current progress in the exciting new field of performance genetics.
Isotropic high resolution optoacoustic imaging with linear detector arrays in bi‐directional scanning (2014)

Optoacoustic (photoacoustic) imaging is often performed with one-dimensional transducer arrays, in analogy to ultrasound imaging. Optoacoustic imaging using linear arrays offers ease of implementation but comes with several performance drawbacks, in particular poor elevation resolution, i.e. the resolution along the axis perpendicular to the focal plane. Herein, we introduce and investigate a bi-directional scanning approach using linear arrays that can improve the imaging performance to quasi-isotropic transverse resolution. We study the approach theoretically and perform numerical simulations and phantom measurements to evaluate its performance under defined conditions. Finally, we discuss the features and the limitations of the proposed method.
Optical implementation of an associative neural network model with a stochastic process (1989)

An optical associative neural network with a stochastic thresholding procedure has been demonstrated. The use of stochastic processing drastically improved the convergence rate into the correct global minima (recognition rate). The properties of undesirable spurious minima were also investigated. It was found that the spurious minima were represented as the mixed states of the stored vectors. A useful method to estimate the required noise level to vanish the spurious minima is described.
The development of an aerodynamic shoe sampling system (2011)

In collaboration with the Transportation Security Laboratory, the National Institute of Standards and Technology has been developing a prototype shoe sampling system that relies on aerodynamic sampling for liberating, transporting, and collecting explosive contamination. Here, we focus on the measurement science of aerodynamic sampling with the goal of uncovering the underlying physics of the flow fields within these sampling systems. This paper will cover the results of a series of experiments that were used to help with the design of our prototype shoe sampling system. Laser light-sheet flow visualization revealed the bulk fluid motion inside and around the sampling system. Polymer microsphere particle standards were used to quantify the particle release efficiency of the shoe sampling system. Patches containing a known mass of explosives were also used to determine the effectiveness of particle release in the shoe sampler. Results from these experiments indicate that particle removal efficiency at a specific location is strongly influenced by its distance from an air jet and the type of explosive or material on the surface. The successful application of these flow visualization techniques and other metrology tools has helped us construct the sampling portion of a shoe screening prototype. The hope is that these tools will be useful to others who are developing next-generation aerodynamic sampling technologies.
Electrical musical instrument (1984)

Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter Facebook Reddit LinkedIn Tools Icon Tools Reprints and Permissions Cite Icon Cite Search Site Citation Reinhard Franz, Wilfried Dittmar; Electrical musical instrument. J. Acoust. Soc. Am. 1 December 1984; 76 (6): 1879. https://doi.org/10.1121/1.391527 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAcoustical Society of AmericaThe Journal of the Acoustical Society of America Search Advanced Search |Citation Search
Broad-Spectrum Noncompetitive Immunocomplex Immunoassay for Cyanobacterial Peptide Hepatotoxins (Microcystins and Nodularins) (2016)

A broad-spectrum noncompetitive immunoassay allowing sensitive and simple detection of a group of similar compounds would be an ideal tool for screening low-molecular weight analytes (<2000 Da) having many variants. However, the development of an essential antibody pair capable of sandwich-type recognition of the analytes' small generic core structure is a demanding task due to limited space available for simultaneous binding of two different antibodies. We report here a generic noncompetitive assay for cyanobacterial microcystins (MCs) and nodularins (Nod), a group of structurally related small cyclic peptides (∼1000 Da) with more than 100 naturally occurring analogs. The assay is based on the unique combination of a generic anti-immunocomplex (anti-IC) single-chain fragment of antibody variable domain (scFv) and a monoclonal antibody capable of binding to an Adda-group (3-amino-9-methoxy-2,6,8-trimethyl-10-phenyldeca-4(E),6(E)-dienoic acid) present in all MCs/Nod. The anti-IC scFv was isolated from a large synthetic antibody library with phage display and used to develop a single-step sandwich-type noncompetitive immunocomplex assay. The sensitive time-resolved immunofluorometry-based assay is capable of detecting all the 11 tested commonly occurring hepatotoxins (MC-LR, -dmlR, -RR, -dmRR, -LA, -LY, -LF, -LW, -YR, -WR, and Nod-R) at concentration below 0.1 μg/L in a 1 h assay. Using MC-LR, the most studied toxic and widely distributed of the toxins, the calculated detection limits (based on blank + 3SD response) are ∼0.026 μg/L in 1 h and ∼0.1 μg/L in 10 min assay time. This is by far the fastest reported immunoassay for MCs and Nod with a detection limit far below the World Health Organization's guideline limit (1 μg/L of MC-LR equivalent in drinking water). The assay was validated with spiked tap and lake water as well as with environmental surface water samples. The developed assay provides a simple, rapid, and highly sensitive tool for the quantitative detection of MCs/Nod with the additional benefit of automation and high-throughput possibilities for large scale screening of drinking and environmental surface water samples. Furthermore, the study describes the first demonstration of the assay intended for the detection of an analyte group comprising similar low-molecular weight compounds exhibiting the benefits of a reagent excess type assay.
Chelated Ruthenium Catalysts for <i>Z</i>-Selective Olefin Metathesis (2011)

We report the development of ruthenium-based metathesis catalysts with chelating N-heterocyclic carbene (NHC) ligands that catalyze highly Z-selective olefin metathesis. A very simple and convenient procedure for the synthesis of such catalysts has been developed. Intramolecular C-H bond activation of the NHC ligand, promoted by anion ligand substitution, forms the appropriate chelate for stereocontrolled olefin metathesis.
Non-Invasive Hemoglobin Monitoring during Hemorrhage and Hypovolemic Shock (2004)

Serial blood draws for the assessment of a trauma patient's hemoglobin (sHgb) and hematocrit (sHct) is standard practice. In the event of multiple casualties this process can be time consuming and lead to the inefficient use of valuable resources. A device that would allow for continuous real-time, non-invasive monitoring of hemoglobin and tissue perfusion would not only improve the utilization of scarce and valuable resources but would also improve triage efforts. We developed a device utilizing the technology of Diffuse Optical Spectroscopy (DOS) to obtain non-invasive measurements of tissue hemoglobin concentration (THC) and oxygen consumption in an animal model of hypovolemic shock induced by successive blood withdrawals. Measured DOS results were compared against invasive systemic physiological measurements to demonstrate that DOS provides a reliable non-invasive measurement of tissue THC, and also quantifies various degrees of hemorrhage induced systemic hypovolemia and subsequent tissue perfusion decreases. DOS provides a potential platform for reliable non-invasive measurements of tissue oxygenated and deoxygenated hemoglobin and may accurately reflect the degree of systemic hypovolemia and compromised tissue perfusion.
Effect of grid support functions and VRT/FRT capability on autonomous anti-islanding schemes in photovoltaic converters (2016)

The high penetration of utility interconnected photovoltaic systems is is leading to a need for inverters to include grid support functions, to minimize the negative impact these variable distributed energy resources may have on system voltage and frequency. Unfortunately, grid support functions may interfere with island detection algorithms; specifically, it may be difficult for an island detection scheme to detect voltage and frequency deviations if that converter and other converters on the same bus actively modulate their real and reactive power outputs in response to voltage and frequency deviations while also tolerating greater deviation. This report provides analysis and simulation evidence to investigate the effect of advanced inverter functions on the performance of island detection schemes. A mitigation scheme is also presented and shown to be effective in simulation.
Anion-Complexation-Induced Stabilization of Charge Separation (2009)

A supramolecular oligochromophoric system possessing exclusive binding sites for both a guest electron acceptor and an anionic cofactor species is developed, and anion-binding-induced stabilization of the charge-separated (CS) state is demonstrated. Toward this, intramolecular and intermolecular photochemical processes of a supramolecular complex of a bis-porphyrinyl-substituted oxoporphyrinogen with a bis(4-pyridyl)-substituted fullerene were investigated by using femtosecond and nanosecond laser flash photolysis measurements. Transient absorption spectra of the supramolecular complex obtained by femtosecond laser flash photolysis indicate that efficient electron transfer occurs from the porphyrin moiety to the fullerene moiety, followed by faster back electron transfer to the ground state. Binding of several different anionic species at the pyrrole amine groups of an oxoporphyrinogen unit within the supramolecular complex was found to improve the rate of the photoinduced electron transfer due to the favorable structural change. The anion binding also improves persistence of the photoinduced CS state between the anion-bound oxoporphyrinogen and fullerene moieties, which is produced by intermolecular electron transfer from the triplet excited state of free porphyrin molecules to free fullerene molecules, as indicated by the nanosecond laser flash photolysis measurements. In the case of fluoride anion binding, anion-complexation-induced stabilization of charge separation gave a 90-fold elongation of the CS state lifetime from 163 ns to 14 micros. Complexation with other anions (acetate or dihydrogen phosphate) also resulted in stabilization of the CS state, whereas weakly bound perchlorate anions gave no improvement. Complexation of anions to the oxoporphyrinogen center lowers its oxidation potential by nearly 600 mV, creating an intermediate energy state for charge migration from the ZnP(*+) to the oxoporphyrinogen:anion complex. An increase in reorganizational energy of electron transfer combined with the decrease in charge recombination driving force caused by anion binding results in an increase in the lifetime of the CS state.
Desulfurization of Hydrocarbons by Electron Transfer Oxidative Polymerization of Heteroaromatic Sulfides Catalyzed by H<sub>5</sub>PV<sub>2</sub>Mo<sub>10</sub>O<sub>40</sub> Polyoxometalate (2011)

Gone in 7200 Seconds: Heteroaromatic thiophene derivatives, refractory to hydrodesulfurization, are removed from hydrocarbons by oxidative polymerization over H5PV2Mo10O40/SiO2 as a recyclable, heterogeneous catalyst. The levels of sulfur-containing compounds reach sub-ppm to nondetectable levels, as analyzed by gas chromatography with a flame photometric detector.
Development of a live attenuated bivalent oral vaccine against Shigella sonnei shigellosis and typhoid fever (2016)

Shigella sonnei and Salmonella Typhi cause significant morbidity and mortality. We exploited the safety record of the oral, attenuated S. Typhi vaccine (Ty21a) by using it as a vector to develop a bivalent oral vaccine to protect against S. sonnei shigellosis and typhoid fever. We recombineered the S. sonnei form I O-antigen gene cluster into the Ty21a chromosome to create Ty21a-Ss, which stably expresses S. sonnei form I O antigen. To enhance survivability in the acid environment of the stomach, we created an acid-resistant strain, Ty21a-AR-Ss, by inserting Shigella glutaminase-glutamate decarboxylase systems coexpressed with S. sonnei form I O-antigen gene. Mice immunized intranasally with Ty21a-AR-Ss produced antibodies against S. sonnei and S. Typhi, and survived lethal intranasal S. sonnei challenge. This paves the way for proposed good manufacturing practices manufacture and clinical trials intended to test the clinical effectiveness of Ty21a-AR-Ss in protecting against S. sonnei shigellosis and typhoid fever, as compared with the current Ty21a vaccine.
Investigations using floating-gate circuits for flash ADCs (2004)

Typically, high speed analog to digital converters are built using various forms of flash converter architectures. However, all of these structures suffer from one major flaw: offsets. Whether these offsets are introduced from the resistive biasing network, mismatches in the comparators, or from other sources, extensive care must be taken to compensate for them. To compensate for these offsets, we investigated the use of floating-gate circuits in a 6-bit flash ADC. By using the floating-gate circuits we are able to program the necessary reference voltages instead of depending on a resistive biasing network. The floating-gate circuits also enable us to compensate for any other offsets. In addition, programming the reference voltages reduces the power dissipation required for biasing the comparator array, and it allows us to implement nonlinear codes. In fact, we can implement any monotonic coding scheme including /spl mu/-law, exponential, and others.