gebhart/patrepeval huggingface dataset:
Designer α1,6-Fucosidase Mutants Enable Direct Core Fucosylation of Intact N-Glycopeptides and N-Glycoproteins (2017)

Core fucosylation of N-glycoproteins plays a crucial role in modulating the biological functions of glycoproteins. Yet, the synthesis of structurally well-defined, core-fucosylated glycoproteins remains a challenging task due to the complexity in multistep chemical synthesis or the inability of the biosynthetic α1,6-fucosyltransferase (FUT8) to directly fucosylate full-size mature N-glycans in a chemoenzymatic approach. We report in this paper the design and generation of potential α1,6-fucosynthase and fucoligase for direct core fucosylation of intact N-glycoproteins. We found that mutation at the nucleophilic residue (D200) did not provide a typical glycosynthase from this bacterial enzyme, but several mutants with mutation at the general acid/base residue E274 of the Lactobacillus casei α1,6-fucosidase, including E274A, E274S, and E274G, acted as efficient glycoligases that could fucosylate a wide variety of complex N-glycopeptides and intact glycoproteins by using α-fucosyl fluoride as a simple donor substrate. Studies on the substrate specificity revealed that the α1,6-fucosidase mutants could introduce an α1,6-fucose moiety specifically at the Asn-linked GlcNAc moiety not only to GlcNAc-peptide but also to high-mannose and complex-type N-glycans in the context of N-glycopeptides, N-glycoproteins, and intact antibodies. This discovery opens a new avenue to a wide variety of homogeneous, core-fucosylated N-glycopeptides and N-glycoproteins that are hitherto difficult to obtain for structural and functional studies.
Lung Cancer: Nebulized Gadolinium-Based Nanoparticles: A Theranostic Approach for Lung Tumor Imaging and Radiosensitization (Small 2/2015) (2015)

The direct administration of Gadolinium-based theranostic nanoparticles (USRPs) into the airways is described on page 215 by J.-L. Coll and co-workers. This work enables the deposition of USRPs around tumor nodules, their multimodal detection (fluorescence and MRI) and, due to the USRPs' radiosensitizing properties, augments significantly the impact of radiotherapy and consequent survival.
Conveyor-belt method for assembling microparticles into large-scale structures using electric fields (2007)

The authors propose and experimentally demonstrate a conveyor-belt method appropriate for building large-scale microparticle structures by sequentially energizing electrodes to aggregate the particles into predetermined locations and then to translate them collectively to a work area for final assembly. This approach employs collective phenomena in a negatively polarized suspension exposed to a high-gradient strong ac electric field.
Frequency-domain ultrasound waveform tomography breast attenuation imaging (2016)

Ultrasound waveform tomography techniques have shown promising results for the visualization and characterization of breast disease. By using frequency-domain waveform tomography techniques and a gradient descent algorithm, we have previously reconstructed the sound speed distributions of breasts of varying densities with different types of breast disease including benign and malignant lesions. By allowing the sound speed to have an imaginary component, we can model the intrinsic attenuation of a medium. We can similarly recover the imaginary component of the velocity and thus the attenuation. In this paper, we will briefly review ultrasound waveform tomography techniques, discuss attenuation and its relations to the imaginary component of the sound speed, and provide both numerical and ex vivo examples of waveform tomography attenuation reconstructions.
Impact of maternal probiotic-supplemented dietary counselling on pregnancy outcome and prenatal and postnatal growth: a double-blind, placebo-controlled study (2010)

The perinatal nutritional environment impacts upon the health and well-being of mother and child also in the long term. The aim of the present study was to determine the safety and efficacy of perinatal probiotic-supplemented dietary counselling by evaluating pregnancy outcome and fetal and infant growth during the 24 months' follow-up. Altogether, 256 women were randomised at their first trimester of pregnancy into a control and a dietary intervention group. The intervention group received intensive dietary counselling provided by a nutritionist and were further randomised, double-blind to receive probiotics (Lactobacillus rhamnosus GG and Bifidobacterium lactis Bb12; diet/probiotics) or placebo (diet/placebo). Firstly, probiotic intervention reduced the frequency of gestational diabetes mellitus (GDM); 13 % (diet/probiotics) v. 36 % (diet/placebo) and 34 % (control); P = 0.003. Secondly, the safety of this approach was attested by normal duration of pregnancies with no adverse events in mothers or children. No significant differences in prenatal or postnatal growth rates among the study groups were detected. Thirdly, distinctive effects of the two interventions were detected; probiotic intervention reduced the risk of GDM and dietary intervention diminished the risk of larger birth size in affected cases; P = 0.035 for birth weight and P = 0.028 for birth length. The results of the present study show that probiotic-supplemented perinatal dietary counselling could be a safe and cost-effective tool in addressing the metabolic epidemic. In view of the fact that birth size is a risk marker for later obesity, the present results are of significance for public health in demonstrating that this risk is modifiable.
ChemInform Abstract: Cross‐Coupling Reactions Catalyzed by Iron Group Metals and N‐Heterocyclic Carbenes via Nonconventional Reaction Mechanisms (2012)

Abstract Review: ca. 100 refs.
Characterization Of Polymeric Nonlinear Optical Materials (1988)

The development of organic nonlinear optical materials requires the accurate measurement of its nonlinear optical and electrooptical properties. These measurements provide a guide to the synthesis and fabrication of new nonlinear organic materials with improved properties. A new technique for the electrooptical characterization of thin polymeric films is presented. This technique is used to measure the linear electrooptical Pockels effect in poled MNA/PMMA guest host glassy polymers. These results are compared to x(2) values derived from second harmonic generation studies. D. C. Kerr effect results are reported for a novel sol gel glass/PMMA composite material containing MNA. These results probe the relative mobility of MNA in PMMA at room temperature and elevated temperatures. x(3) values of several organic liquids are also measured by third harmonic generation studies in air. These values are compared with those derived from measurements in vacuum and good agreement is found between the two techniques.
SMILE (2007)

In this paper, we describe the SMILE (Smart MIddleware, Light Ends) system which is one of the earliest systems built in the area of distributed event stream processing. SMILE unites the "publish-subscribe" model of messaging middleware with the "continuous query" model of database systems. In SMILE, information producers, which may be sensors, applications, or databases, generate streams of events, such as RFID data, news items or stock trades; consumers specify stateful subscriptions to derived views, such as "individuals trading top 5 total volume of stock Y within x minutes before a major news story about the same company Y"; the SMILE system constructs and deploys a dataflow network of computations which process events from producers, compute derived views and deliver continuous and timely updates of subscribed views to consumers. Research challenges addressed by SMILE include: rigorously defining the semantics for correct state delivery; implementing this semantics in the presence of failure; distributing computations optimally over a network of multiple servers; scheduling and controlling message flow in this network; smoothly integrating this technology with other components, e.g., databases, services, user interfaces. SMILE is applicable to any "sense and respond" scenario that requires monitoring and processing of events as they are generated. Applications span multiple industries, e.g., monitoring financial opportunities and detecting potential fraud in financial industry, systems management and alerts in data centers, inventory management in RFID applications, and business performance management. In this paper, we describe the SMILE system, its architecture, services supported, and implementation details, and its use in multiple application scenarios.
Super Resolution Ultrasound using Recursive Imaging of Highly Dense Scatterers (2022)

Microbubbles (MBs) tracking is an integral part of super-resolution ultrasound imaging (SRI). In SRI, MBs are tracked to make intensity and velocity maps. These MBs must be sparse and separable to make MBs tracking reliable for a desired intensity and velocity maps. However, the sparse distribution of the MBs necessitates several minutes long acquisitions to go through the whole circulation. Because of abundance of targets in SUper Resolution ultrasound imaging with Erythrocyte (SURE), it is possible to make a super resolution image in just seconds instead of several minutes in SRI with MBs. That means increasing the number of scatterers could reduce the acquisition time, however tracking of high density scatterers is quite challenging. This paper hypothesizes that non-separable target traveling can yield super resolution using a fast recursive synthetic aperture imaging technique. A phantom with four tube pairs are simulated using Field II with 175 scatterers per resolution cell and also with realistic tissue motion measured in-vivo from a rat kidney. For simulation, the impulse response and characteristics of GE L8-18iD probe is used. The wavelength is 154 <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">$\mu \mathrm{m}$</tex> . A synthetic aperture imaging sequence is consisting of 12 emissions. Two seconds of data is acquired by a frame rate of 208.3 Hz. Recursive imaging is used to increase the frame rate to 2500 Hz. Motion compensation and echo-cancelling is applied to the beamformed data to tracking detected peaks for creating the velocity map. The velocity maps and profiles showed estimated directions and velocities for the four tube pairs in two opposite flow directions. The velocity map and profiles showed that all tube pairs with 200, 100, 50, and 25 <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">$\mu \mathrm{m}$</tex> are separated correctly for 2 seconds instead of several minutes. Errors between actual and estimated vessel center distances are 6.6, 5.1, 2.6, and 1.7 <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">$\mu \mathrm{m}$</tex> , respectively. Recursive imaging thus makes it is possible to obtain a super-resolution image of highly dense scatterers not only for tube distances less than the diffraction limit of half of a wavelength but also less than a quarter of a wavelength in just 2 seconds.
Energy‐Efficient Hydrogen Separation by AB‐Type Ladder‐Polymer Molecular Sieves (2014)

Increases in hydrogen selectivity of more than 100% compared with the most selective ladder polymer of intrinsic microporosity (PIM) reported to date are achieved with self-polymerized A-B-type ladder monomers comprising rigid and three-dimensional 9,10-dialkyl-substituted triptycene moieties. The selectivities match those of materials commercially employed in hydrogen separation, but the gas permeabilities are 150-fold higher. This new polymer molecular sieve is also the most selective PIM for air separation.
Identification of a WT1 protein‐derived peptide, WT1<sub>187</sub>, as a HLA‐A*0206‐restricted, WT1‐specific CTL epitope (2008)

The Wilms' tumor gene WT1 is overexpressed in various kinds of hematopoietic malignancies as well as solid cancers, and this protein has been demonstrated to be an attractive target antigen for cancer immunotherapy. WT1-specific CTL epitopes with a restriction of HLA-A 2402 or HLA-A 0201 have been already identified. In the present study it has been demonstrated that a 9-mer WT1-derived WT1(187) peptide, which had already been shown to elicit a WT1-specific CTL response with a restriction of HLA-A 0201, can also elicit a CTL response with a restriction of HLA-A 0206. In all three different HLA-A 0206(+) healthy donors examined, WT1(187) peptide-specific CTL could be generated from peripheral blood mononuclear cells, and the CTL showed cytotoxic activity that depended on dual expression of WT1 and HLA-A 0206 molecules. The present study describes the first identification of a HLA-A 0206-restricted, WT1-specific CTL epitope. The present results should help to broaden the application of WT1 peptide-based immunotherapy from only HLA-A 0201-positive to HLA-A 0206-positive cancer patients as well.
One‐Pot Production of Enantiopure Alkylamines and Arylalkylamines of Opposite Chirality Catalyzed by ω‐Transaminase (2013)

Looking for favors: The thermodynamically favorable asymmetric amination of alkyl ketones was catalyzed by ω-transaminase with racemic arylalkylamines as an amine donor, thus affording enantiopure alkylamines and arylalkylamines of opposite chirality.
Immunological Characterization of Novel Secreted Antigens of <i>Mycobacterium tuberculosis</i> (2005)

Proteins secreted by Mycobacterium tuberculosis are targets of host immune responses and as such are investigated for vaccine and immunodiagnostics development. Computer-driven searches of the M. tuberculosis H37Rv genome had previously identified 45 novel secreted proteins. Here, we report the characterization of these antigens in terms of specificity for the M. tuberculosis complex and the ability to induce human immune responses. BLAST homology searches and Southern hybridization identified 10 genes that were either specific for the M. tuberculosis complex or found in only two nontuberculous mycobacterial species of minor medical significance. Selected recombinant proteins were purified from Escherichia coli cells and tested for the ability to elicit antibody responses in tuberculosis patients. Reactivity of the serum panel was ' 36% with at least one of five novel proteins (Rv0203, Rv0603, Rv1271c, Rv1804c and Rv2253), 56% with the 38 kDa lipoprotein, a M. tuberculosis antigen known to be highly seroreactive, and 68% with a combination of Rv0203, Rv1271c and the 38 kDa antigen. Thus, at least five novel secreted proteins induce antibody responses during active disease; some of these proteins may increase the sensitivity of serological assays based on the 38 kDa antigen.
Bonding Wood Veneers with Cellulose Solvents (1984)

Abstract Various solvent systems capable of dissolving cellulose have been reported in the literature. Cuene (cupriethylene diamine hydroxide) and FeTNa (ferric sodium tartrate) are well known examples. Preliminary experiments were conducted with Cuene and FeTNa to determine if the cellulose dissolving ability of these systems could be used to bond yellow birch veneers. The results indicate that relatively high dry shear strengths, and low wet shear strengths are obtained.
Laminated Laser-Induced Graphene Composites (2020)

Laser-induced graphene (LIG) is a porous graphene foam generated by lasing carbon-based precursors. Compositing LIG expands the spectrum of applications for which the material may be used. Techniques for scale-up of LIG composites will be essential as the technology approaches commercialization. Roll-to-roll processing is of special interest, as precisely controlled patterning can be performed in conjunction with continuous formation of composites. Here, we demonstrate a simple lamination compositing method that is compatible with roll-to-roll processing and yields functional, patterned, and multilayered LIG composites with various thermoplastic films. Multiple lamination steps are used to encapsulate LIG within composites. We also demonstrate several applications for LIG that have been enabled by the lamination compositing technique. These include robust flexible electrodes generated through laminating copper foil strips into the LIG composite, LIG-based triboelectric nanogenerators to harvest waste mechanical energy, antimicrobial LIG composite bandages with varying hydrophobicity, and LIG puncture detectors.
Resilient two dimensional redundancy based fault-tolerant controller array for modular multi-level converters (2017)

In this paper, a state-of-the-art controller architecture for controlling modular multi-level converter (MMC) family is proposed. By using this controller architecture, single point of failure in the power converter, controller board or the communication network does not interrupt the functionality of the system. In order to achieve that, a two-dimensional array of controllers are formed to handle the MMC. In this architecture, a selected master controller is synchronizing slave controllers. In addition, adjacent slave controllers check the internal functionality of each other. In case of failure detection, the failed component will be isolated from system and the converter may continue its operation without interruption. This paper covers synchronization mechanism for slave controllers, master controller selection, and presents experimental results for the proposed controller.
Photometer Tracks The Sun (1988)

Airborne Sun-tracking photometer enables observations of Sun during much greater portion of flights than previously possible, without special maneuvers of airplane. Instrument occupies dome atop airplane. Fiberglass dome protects photometer and rotates to aim photometer in azimuth and elevation to track Sun. Provides controlled environment for instrument, including mechanical and electronic parts. Instrument calibrated without removing it from airplane.
Monolithic Electrically Injected Nanowire Array Edge-Emitting Laser on (001) Silicon (2014)

A silicon-based laser, preferably electrically pumped, has long been a scientific and engineering goal. We demonstrate here, for the first time, an edge-emitting InGaN/GaN disk-in-nanowire array electrically pumped laser emitting in the green (λ = 533 nm) on (001) silicon substrate. The devices display excellent dc and dynamic characteristics with values of threshold current density, differential gain, T0 and small signal modulation bandwidth equal to 1.76 kA/cm(2), 3 × 10(-17) cm(2), 232 K, and 5.8 GHz respectively under continuous wave operation. Preliminary reliability measurements indicate a lifetime of 7000 h. The emission wavelength can be tuned by varying the alloy composition in the quantum disks. The monolithic nanowire laser on (001)Si can therefore address wide-ranging applications such as solid state lighting, displays, plastic fiber communication, medical diagnostics, and silicon photonics.
Thermally stable, superlattice-enhanced 1.3- mu m InGaAs IMSM photodetectors on GaAs substrates (1991)

Dark current, DC responsivity, and high-frequency response data of 1.3- mu m interdigitated metal-semiconductor-metal (IMSM) photodetectors on a thermally stable, superlattice-enhanced InGaAs/GaAs structure are reported. Auger analysis revealed that the superlattice cap layer is capable of inhibiting the out-diffusion of indium to the surface during high-temperature annealing cycle, thus minimizing the degradation of the optical and electrical characteristics of the photodetectors fabricated on this material. The internal quantum efficiency and high-frequency bandwidth of the detectors are over 91% and 14 GHz, respectively. The stability of this IMSM structure to high-temperature annealing offers the potential for fabrication of low-cost, long-wavelength monolithic receivers based oil GaAs MESFET technology.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Investigation of parameters affecting treatment time in MRI-guided transurethral ultrasound therapy (2010)

Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter Facebook Reddit LinkedIn Tools Icon Tools Reprints and Permissions Cite Icon Cite Search Site Citation W. A. N'Djin, M. Burtnyk, R. Chopra, M. J. Bronskill; Investigation of parameters affecting treatment time in MRI‐guided transurethral ultrasound therapy. AIP Conf. Proc. 9 March 2010; 1215 (1): 323–326. https://doi.org/10.1063/1.3367172 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAIP Publishing PortfolioAIP Conference Proceedings Search Advanced Search |Citation Search
Four‐dimensional transcatheter intraarterial perfusion (TRIP)‐MRI for monitoring liver tumor embolization in VX2 rabbits (2008)

Transcatheter intraarterial perfusion (TRIP)-MRI is an intraprocedural technique to iteratively monitor liver tumor perfusion changes during transcatheter arterial embolization (TAE) and chemoembolization (TACE). However, previous TRIP-MRI approaches using two-dimensional (2D) T(1)-weighted saturation-recovery gradient-recalled echo (GRE) sequences provided only limited spatial coverage and limited capacity for accurate perfusion quantification. In this preclinical study, a quantitative 4D TRIP-MRI technique (serial iterative 3D volumetric perfusion imaging) with rigorous radiofrequency (RF) B(1) field calibration and dynamic tissue longitudinal relaxation rate R(1) measurement is presented for monitoring intraprocedural liver tumor perfusion during TAE. 4D TRIP-MRI and TAE were performed in five rabbits with eight VX2 liver tumors (N = 8). After B(1) calibrated baseline and dynamic R(1) quantification, subsequent tissue contrast agent concentration time curves were derived. A single-input flow-limited pharmacokinetic model and peak gradient method were applied for perfusion analysis. The perfusion Frho reduced significantly from pre-TAE 0.477 (95% confidence interval [CI]: 0.384-0.570) to post-TAE 0.131 (95% CI: 0.080-0.183 ml/min/ml, P < 0.001).
Aerogels, emulsions, and composites: Controlling structure with organic sol-gel chemistry (1992)

The sol-gel polymerization of resorcinol with formaldehyde is a proven synthetic route for the formation of organic aerogels. These materials can also be pyrolyzed in an inert atmosphere to form vitreous carbon aerogels. Both resorcinol-formaldehyde and carbon aerogels are characterized by high porosity (50--98%), ultrafine cell/pore sizes (< 100 mn), high surface area (400--1000 m{sup 2}/g), and a solid matrix composed of interconnected colloidal-like particles or fibrous chains with characteristic diameters of 10 nm. While this nanostructure and the low Z (atomic number) composition are responsible for the unique optical, thermal, acoustic, mechanical, and electrical properties of organic aerogels, there are certain applications in which a larger scale structure is also desired in combination with the unique features of the aerogel. In order to achieve this goal, we have been emulsifying our resorcinol-formaldehyde sols to form foams which retain the aerogel framework but have some cells/pores as large as 2 {mu}m. In another approach, we have infused an emulsion-derived polystryene foam with a resorcinol-formaldehyde sol to form a new composite foam. This paper addresses the chemistry-structure-property relationships of organic aerogels, their emulsion-derived derivatives, and composite foams.