gebhart/patrepeval huggingface dataset:
<i>Vipera lebetina</i> Venom Contains All Types of Snake Venom Metalloproteases (2005)

Snake venoms contain four classes of metalloproteases that all have a typical zinc-chelating sequence (HEXXHGXXH). N-terminal sequences and internal sequences of different purified metalloproteases were determined using Edman sequencing and LC MS/MS technique. Oligonucleotides were designed and used as primers for cDNA cloning from Vipera lebetina venom gland cDNA library. We found that isoforms of fibrinolytic enzyme lebetase Le-4 and Le-3 are synthesized in different way: Le-4 is synthesized as P-I type metalloprotease, Le-3 is synthesized with disintegrin-like domain as P-II type protease and processed post-translationally. An endothelial cell apoptosis-inducing heterodimeric glycosylated metalloprotease, V. lebetina apoptosis-inducing protease (VLAIP), belongs to P-III type containing metalloprotease, disintegrin-like and cysteine-rich domains. All these enzymes hydrolyze the Aalpha-chain and more slowly the Bbeta-chain of fibrinogen. Treatment of HUVEC cells with VLAIP induces changes in the attachment of cells to the substrate and causes apoptosis. V. lebetina venom contains also P-IV type-specific coagulant factor X activator (VLFXA) that cleaves the Arg52-Ile53 bond in the heavy chain of human factor X. VLFXA is a glycoprotein composed of a heavy chain and two C-type lectin-like light chains linked by disulfide bonds. The heavy and light chains of VLFXA are synthesized from different genes.
Development of physical models for the simulation of optical properties of solar cell modules (2015)

[no abstract]
Distinct mucosal immunopathologic profiles in atopic and nonatopic chronic rhinosinusitis without nasal polyps in Central China (2016)

Atopic and nonatopic CRSsNP have distinct mucosal immunopathologic profiles. CRSsNP is a heterogeneous disorder consisting of multiple groups of biological subtypes, or "endotypes," which may argue for different therapeutic strategies.
An algorithm for music recommendation based on the user's musical preferences and desired emotions (2018)

Studies show that music is an effective form of emotional induction and can change the emotional behavior of the user. In view of this, this article seeks to create a "selection" algorithm that includes a subset of songs chosen by the user, while taking account of their current emotional state and allowing them to achieve the emotional state that is desired. In carrying out the experiment, we established the current emotional state, the desired emotional state and 20 suggestions of musical items from a sample of 20 users. With the aim of achieving the desired emotional state, the proposed algorithm returned a new list of musical items for each participant. The participants underwent an evaluation during and after playing the music to assess the effectiveness of the returned list. The results suggest that when listening to the songs selected by the algorithm, the user can approach the desired emotional state.
Human sweet taste receptor mediates acid-induced sweetness of miraculin (2011)

Miraculin (MCL) is a homodimeric protein isolated from the red berries of Richadella dulcifica. MCL, although flat in taste at neutral pH, has taste-modifying activity to convert sour stimuli to sweetness. Once MCL is held on the tongue, strong sweetness is sensed over 1 h each time we taste a sour solution. Nevertheless, no molecular mechanism underlying the taste-modifying activity has been clarified. In this study, we succeeded in quantitatively evaluating the acid-induced sweetness of MCL using a cell-based assay system and found that MCL activated hT1R2-hT1R3 pH-dependently as the pH decreased from 6.5 to 4.8, and that the receptor activation occurred every time an acid solution was applied. Although MCL per se is sensory-inactive at pH 6.7 or higher, it suppressed the response of hT1R2-hT1R3 to other sweeteners at neutral pH and enhanced the response at weakly acidic pH. Using human/mouse chimeric receptors and molecular modeling, we revealed that the amino-terminal domain of hT1R2 is required for the response to MCL. Our data suggest that MCL binds hT1R2-hT1R3 as an antagonist at neutral pH and functionally changes into an agonist at acidic pH, and we conclude this may cause its taste-modifying activity.
Discrimination between the human prostate normal and cancer cell exometabolome by GC-MS (2018)

Serum prostate-specific antigen (PSA) is currently the most used biomarker in clinical practice for prostate cancer (PCa) detection. However, this biomarker has several drawbacks. In this work, an untargeted gas chromatography-mass spectrometry (GC-MS)-based metabolomic profiling of PCa cells was performed to prove the concept that metabolic alterations might differentiate PCa cell lines from normal prostate cell line. For that, we assessed the differences in volatile organic compounds (VOCs) profile in the extracellular medium (exometabolome) of four PCa cell lines and one normal prostate cell line at two pH values (pH 2 and 7) by GC-MS. Multivariate analysis revealed a panel of volatile metabolites that discriminated cancerous from normal prostate cells. The most altered metabolites included ketones, aldehydes and organic acids. Among these, we highlight pentadecane-2-one and decanoic acid, which were significantly increased in PCa compared to normal cells, and cyclohexanone, 4-methylheptan-2-one, 2-methylpentane-1,3-diol, 4-methylbenzaldehyde, 1-(3,5-dimethylfuran-2-yl)ethanone, methyl benzoate and nonanoic acid, which were significantly decreased in PCa cells. The PCa volatilome was markedly influenced by the VOCs extraction pH, though the discriminant capability was similar. Overall, our data suggest that VOCs monitoring has the potential to be used as a PCa screening methodology.
Leveraging the Web Platform for Ambient Computing: An Experience (2008)

Abstract. This paper explores a very simple idea: what can be achieved by using the principles and the technologies of the Web Platform1 when they are applied to Ambient Computing? To answer this question we will present an experience that addresses some of the problems of Am-bient Computing by making use of the technologies and the common practices of today’s Web Platform. The aim is to provide an architecture that tries to lower the deployment costs while guaranteeing accessibility, interoperability and extendibility.
Influence of Medium Exchange Schedules on Metabolic, Growth, and GM‐CSF Secretion Rates of Genetically Engineered NIH‐3T3 Cells (1991)

The metabolic and secretory characteristics of NIH-3T3 fibroblasts transfected with a cDNA encoding human granulocyte-macrophage colony stimulating factor (GM-CSF) were examined as a function of the culture medium exchange schedule. The rates of glucose and glutamine consumption and of lactate and ammonia production were measured over exchange schedules ranging from complete medium replacement weekly (1/week) to complete medium replacement daily (7/week). All measured metabolic rates increased with increased medium exchange rates and accelerated sharply between exchange rates of 3.5/week and 7/week. The lactate/glucose and ammonia/glutamine yield coefficients, however, remained invariant at about 1.9 and 1.0 mol/mol, respectively, under all medium perfusion conditions. A shift-up in medium perfusion rates from 3.5/week to 7/week resulted in increased metabolic rates that resembled those observed in the cultures that were exchanged at the 7/week rate throughout, showing that the metabolic rates could be directly controlled by the perfusion rate. Differential regulation of medium versus serum perfusion demonstrated that increased NIH-3T3 cell metabolism was directly proportional to the serum flux to which the cells were exposed. Thus a limiting serum component is responsible for the altered metabolic and growth rates. The GM-CSF production by the transfected 3T3 cells was stable but exhibited substantial transient increases during periods of cell proliferation, demonstrating that the secretion of transfected gene products can be highly modulated even when the cDNA is driven from a constitutive promoter. These studies show that the metabolic and secretory behavior of genetically engineered cells is influenced by the medium exchange schedule.
Evaluation of a 345 nm Femtosecond Laser for Corneal Surgery with Respect to Intraocular Radiation Hazard (2015)

Our analysis confirms theoretical considerations suggesting that ultraviolet femtosecond laser treatment of the cornea is safe for intraocular tissues. Transmitted light including stray light induces no photochemical effects in lens or retina at energy levels much higher than required for the clinical purpose. These conclusions cannot be applied to eyes with pre-existing retinal damage, as these may be more vulnerable to light.
Effectiveness of Collagen–Gentamicin Implant for Treatment of “Dirty” Abdominal Wounds (1999)

The purpose of this work was to compare the efficacy and safety of the collagen-gentamicin sponge with conventional treatment (wound open, maintaining a close observation, and cleaning it daily with antiseptics) for the prophylaxis of infection in "dirty" abdominal wounds. Seventy-three patients with dirty abdominal wounds caused by gastrointestinal tract surgery were studied. The patients were randomized in two groups: group A, open wounds, treated with local cleansing, metronidazole 20 to 40 mg/kg/day IV and gentamicin 5 mg/kg/day IV for 7 days. Group B, primary closure with collagen-gentamicin implant plus metronidazole 20 to 40 mg/kg/day IV for 7 days. Surgical wound infections were significantly reduced by the collagen-gentamicin implant. Polymicrobial infections were observed in group A, whereas the infections were caused only by a single organism in group B. In conclusion, the collagen-gentamicin implant is effective and well tolerated in the treatment of "dirty" surgical abdominal wounds because it significantly reduces the wound infection rate (p < 0.01) and shortens healing time (p < 0.001) and the period of disability.
Use of high-sensitivity troponin for the diagnosis of acute myocardial infarction (2013)

Although there is broad consensus that cardiac troponin I or T (cTn) is the preferred biomarker and complements clinical assessment and the 12-lead ECG in the early diagnosis of acute myocardial infarction, important uncertainties and questions remain on the value of high-sensitivity cTn assays, including their best clinical use. Although these assays have been introduced clinically in many countries, they still await regulatory approval in the USA. This review summarizes the recent advances and potential pitfalls in the clinical application of high-sensitivity cTn assays in the early diagnosis of acute myocardial infarction.
ERRATA, VOL. II (2013)

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Growth and Methane Oxidation Rates of Anaerobic Methanotrophic Archaea in a Continuous-Flow Bioreactor (2003)

Anaerobic methanotrophic archaea have recently been identified in anoxic marine sediments, but have not yet been recovered in pure culture. Physiological studies on freshly collected samples containing archaea and their sulfate-reducing syntrophic partners have been conducted, but sample availability and viability can limit the scope of these experiments. To better study microbial anaerobic methane oxidation, we developed a novel continuous-flow anaerobic methane incubation system (AMIS) that simulates the majority of in situ conditions and supports the metabolism and growth of anaerobic methanotrophic archaea. We incubated sediments collected from within and outside a methane cold seep in Monterey Canyon, Calif., for 24 weeks on the AMIS system. Anaerobic methane oxidation was measured in all sediments after incubation on AMIS, and quantitative molecular techniques verified the increases in methane-oxidizing archaeal populations in both seep and nonseep sediments. Our results demonstrate that the AMIS system stimulated the maintenance and growth of anaerobic methanotrophic archaea, and possibly their syntrophic, sulfate-reducing partners. Our data demonstrate the utility of combining physiological and molecular techniques to quantify the growth and metabolic activity of anaerobic microbial consortia. Further experiments with the AMIS system should provide a better understanding of the biological mechanisms of methane oxidation in anoxic marine environments. The AMIS may also enable the enrichment, purification, and isolation of methanotrophic archaea as pure cultures or defined syntrophic consortia.
The functional interactome of <scp>PYHIN</scp> immune regulators reveals <scp>IFIX</scp> is a sensor of viral <scp>DNA</scp> (2015)

The human PYHIN proteins, AIM2, IFI16, IFIX, and MNDA, are critical regulators of immune response, transcription, apoptosis, and cell cycle. However, their protein interactions and underlying mechanisms remain largely uncharacterized. Here, we provide the interaction network for all PYHIN proteins and define a function in sensing of viral DNA for the previously uncharacterized IFIX protein. By designing a cell-based inducible system and integrating microscopy, immunoaffinity capture, quantitative mass spectrometry, and bioinformatics, we identify over 300 PYHIN interactions reflective of diverse functions, including DNA damage response, transcription regulation, intracellular signaling, and antiviral response. In view of the IFIX interaction with antiviral factors, including nuclear PML bodies, we further characterize IFIX and demonstrate its function in restricting herpesvirus replication. We discover that IFIX detects viral DNA in both the nucleus and cytoplasm, binding foreign DNA via its HIN domain in a sequence-non-specific manner. Furthermore, IFIX contributes to the induction of interferon response. Our results highlight the value of integrative proteomics in deducing protein function and establish IFIX as an antiviral DNA sensor important for mounting immune responses.
The influence of patient and wound variables on healing of venous leg ulcers in a randomized controlled trial of growth-arrested allogeneic keratinocytes and fibroblasts (2013)

Wound duration is a quantifiable surrogate for one or more undefined variables that can have a profound negative effect on venous leg ulcer healing. Although cell therapy overcame barriers to healing, the only specific barrier identified was the presence of certain bacterial species. Interventional trials of potentially effective new therapies can be most informative when patients with suspected barriers to healing are included. The specific measurement of candidate barriers such as microbial pathogens, wound inflammatory state, and fibroblast function should be considered in future randomized trials to improve our understanding of the basis for chronicity.
MRI analysis of angiogenesis during mouse embryo implantation (2006)

Uterine receptivity and embryo implantation depend on local induction of angiogenesis and vascular permeability. Poor uterine receptivity has been implicated in implantation failure; however, relatively little is known about the mechanism that underlies endometrial vascular hyperpermeability in implantation sites. Here we show that contrast-enhanced (CE)-MRI and fluorescence microscopy using biotin-BSA-GdDTPA allowed high-resolution detection and quantitative assessment of mouse embryo implantation sites as early as embryonic day 4.5 (E4.5), and subsequent vascular expansion at E5.5. Vessel permeability, but not blood volume, was significantly elevated in E4.5 implantation sites relative to nonimplanted uterus, showing that elevation of vascular permeability is a very early response preceding E4.5. A significantly increased blood volume was detected by MRI and fluorescence microscopy in implantation sites between E4.5 and E5.5. On the other hand, despite the increase in blood volume, implantation sites showed only a small nonsignificant further increase in vascular permeability during these 2 days, demonstrating the rapid dynamics of vascular remodeling during the early days of pregnancy. Functional imaging by MRI, as reported here, allows multiparametric measurement of angiogenesis during normal mouse implantation and would facilitate the application of MRI to evaluate involvement of the vasculature in mouse models of impaired implantation.
C/sup 2/: a mixed textual/graphical environment for C (2003)

A visual programming environment for a subset of the C language is described. The C/sup 2/ environment, as it is called, runs on a personal workstation with high-resolution graphics display. Both conventional textual code entry and editing, and program composition by means of an experimental hybrid textual graphical method, are supported and coexist side by side on the screen at all times. The built-in text editor incorporates selected Unix VI commands in conjunction with a C syntax interpreter. Hybrid textual/graphical program composition is facilitated by a BLOX-type environment in which graphical icons represent program structures and text in the icons represents user-supplied parameters attached to those structures. The two representations are coupled, so that modifications entered using either one automatically generate the appropriate update in the other. Although not all of the C language is yet supported. C/sup 2/ is not a toy system. Textual files that contain C programs serve as input and output. Graphical representations serve merely as internally generated aids to the programmers and are not stored between runs.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Microbial influences on the small intestinal response to radiation injury (2010)

New knowledge implies that investigations aimed at deciphering the microbiome-host interactions before and after small bowl radiation injury may eventually allow prediction of disease course and offer opportunities for the development of novel therapeutic or prophylactic strategies.
Radionuclide imaging of primary renal-cell carcinoma by I-131-labeled antitumor antibody. (1978)

A goat antibody against human renal-cell carcinoma reacted on immunofluorescence with renal-cell carcinomas from 20 patients, but not with normal adult human tissues, including kidney. After i.v. administration the I-131-linked antibody showed preferential tumor localization in six of seven patients with primary renal carcinoma. Labeled antitumor antibodies may have the specificity for tumor imaging that current radiopharmaceuticals lack.
Region-based all-in-focus light field rendering (2009)

Light field rendering is an approach to synthesize virtual views of a scene from a set of original images. When minimizing the number of images for rendering, the light field may become under-sampled, leading to aliasing artifacts. To render an under-sampled light field in high quality and without aliasing artifacts is a challenge. We present a light field rendering algorithm with region-based focus to create all-in-focus virtual views. Our algorithm was compared to: (a) ground truth images and (b) a state-of-the-art technique for rendering under-sampled light fields. Extensive rendering experiments confirm that our algorithm provides visible quality improvement, quantified as about 10% RMSE reduction. However, the subjective improvement is larger and it produces images comparable to the ground truth. This algorithm contributes to practical applications of light field rendering, such as image generation in stereoscopic displays and free-viewpoint video (FVV).
Analysis of the Helical Conformations in Poly (.beta.-L-aspartate)s: Poly(.alpha.-n-butyl .beta.-L-aspartate) and Poly[.alpha.-(2-methoxyethyl) .beta.-L-aspartate] (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAnalysis of the Helical Conformations in Poly (.beta.-L-aspartate)s: Poly(.alpha.-n-butyl .beta.-L-aspartate) and Poly[.alpha.-(2-methoxyethyl) .beta.-L-aspartate]Juan J. Navas, Carlos Aleman, Francisco Lopez-Carrasquero, and Sebastian Munoz-GuerraCite this: Macromolecules 1995, 28, 13, 4487–4494Publication Date (Print):June 1, 1995Publication History Published online1 May 2002Published inissue 1 June 1995https://pubs.acs.org/doi/10.1021/ma00117a017https://doi.org/10.1021/ma00117a017research-articleACS PublicationsRequest reuse permissionsArticle Views46Altmetric-Citations33LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Percolation Transitions in Scale-Free Networks under the Achlioptas Process (2009)

It has been recently shown that the percolation transition is discontinuous in Erdos-Rényi networks and square lattices in two dimensions under the Achlioptas process (AP). Here, we show that when the structure is highly heterogeneous as in scale-free networks, a discontinuous transition does not always occur: a continuous transition is also possible depending on the degree distribution of the scale-free network. This originates from the competition between the AP that discourages the formation of a giant component and the existence of hubs that encourages it. We also estimate the value of the characteristic degree exponent that separates the two transition types.
Estimating the Effects of Pavement Condition on Vehicle Operating Costs (2012)

This report presents models for estimating the effects of pavement condition on vehicle operating costs. These models address fuel consumption, tire wear, and repair and maintenance costs and are presented as computational software on the accompanying CD-ROM, CRP-CD-111, to facilitate use. The material contained in the report should be of immediate interest to state pavement, construction, and maintenance engineers; vehicle fleet managers; and those involved in pavement investment decision processes and financial aspects of highway transportation.