gebhart/patrepeval huggingface dataset:
Enterprise model-based software architecture with server component integration (2002)

Many companies are seeking new information systems that support more business activities than current systems. Such a system should enable more rapid information collection and sharing, and promote increased automation. Furthermore, there is a strong technological demand for the integration of various business systems and rapid modification of business processes and rules embedded in the system. To meet this demand, we propose enterprise model-based software development and associated software architecture with integrated server components. An experimental but practical system has been developed and evaluated in projects located in various countries. This paper discusses the viability of enterprise model-based software architecture with integrated server components such as a business application system, a Web server, a workflow server, and a directory server.
Clinical and immunological characteristics of a pediatric population with food protein‐induced enterocolitis syndrome (FPIES) to fish (2015)

FPIES caused by fish in many cases presents with severe clinical manifestations. Patch test has poor diagnostic value, and OFC is the gold standard to test tolerance. The cytokine TNF-α may be implicated in the clinical symptoms. Higher expression of HLA-DR in dendritic cells has also been detected in our patients.
A doubly auxotrophic CHO‐K1 cell line for the production of recombinant monoclonal antibodies (2020)

Chinese hamster ovary (CHO) cells are the most widely used mammalian hosts for recombinant protein production due to their hardiness, ease of transfection, and production of glycan structures similar to those in natural human monoclonal antibodies. To enhance the usefulness of CHO-K1 cells we developed a new selection system based on double auxotrophy. We used CRISPR-Cas9 to knockout the genes that encode the bifunctional enzymes catalyzing the last two steps in the de novo synthesis of pyrimidines and purines (uridine monophosphate synthase and 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase [ATIC], respectively). Survival of these doubly auxotrophic cells depends on the provision of sources of purines and pyrimidines or on the transfection and integration of open reading frames encoding these two enzymes. We successfully used one such double auxotroph (UA10) to select for stable transfectants carrying (a) the recombinant tumor necrosis factor-α receptor fusion protein etanercept and (b) the heavy and light chains of the anti-Her2 monoclonal antibody trastuzumab. Transfectant clones produced these recombinant proteins in a stable manner and in substantial amounts. The availability of this double auxotroph provides a rapid and efficient selection method for the serial or simultaneous transfer of genes for multiple polypeptides of choice into CHO cells using readily available purine- and pyrimidine-free commercial media.
LINGO-1 and AMIGO3, potential therapeutic targets for neurological and dysmyelinating disorders? (2017)

Leucine rich repeat proteins have gained considerable interest as therapeutic targets due to their expression and biological activity within the central nervous system. LINGO-1 has received particular attention since it inhibits axonal regeneration after spinal cord injury in a RhoA dependent manner while inhibiting leucine rich repeat and immunoglobulin-like domain-containing protein 1 (LINGO-1) disinhibits neuron outgrowth. Furthermore, LINGO-1 suppresses oligodendrocyte precursor cell maturation and myelin production. Inhibiting the action of LINGO-1 encourages remyelination both <i>in vitro</i> and <i>in vivo</i>. Accordingly, LINGO-1 antagonists show promise as therapies for demyelinating diseases. An analogous protein to LINGO-1, amphoterin-induced gene and open reading frame-3 (AMIGO3), exerts the same inhibitory effect on the axonal outgrowth of central nervous system neurons, as well as interacting with the same receptors as LINGO-1. However, AMIGO3 is upregulated more rapidly after spinal cord injury than LINGO-1. We speculate that AMIGO3 has a similar inhibitory effect on oligodendrocyte precursor cell maturation and myelin production as with axogenesis. Therefore, inhibiting AMIGO3 will likely encourage central nervous system axonal regeneration as well as the production of myelin from local oligodendrocyte precursor cell, thus providing a promising therapeutic target and an area for future investigation.
Multiple Encounter Simulation for High-acuity Multipatient Environment Training (2007)

Patient safety interventions for multitasking, multipatient, error-prone work settings such as the emergency department (ED) must improve assorted clinical abilities, specific cognitive strategies, and teamwork functions of the staff to be effective. Multiple encounter simulation scenarios explore and convey this specialized mental work-set through use of multiple high-fidelity medical simulation (SIM) manikins in realistic surroundings. Multipatient scenarios reflect the work situations being targeted yet have the benefit of scripted control and instructor guidance to advance specific educational objectives. The use of two or more SIM patients promotes the exploration not only of multiple distinct clinical issues but also of interdependent processes pervasive in EDs. Cascading shortages of time, personnel, equipment, and supplies are re-created, thereby replicating process limitations at various levels, in a safe environment in which compensatory actions and adaptive behaviors can be learned. Distinguishing features of multipatient exercises include 1) broadened educational scope and expanded indications for SIM application, 2) enhanced scenario complexity, 3) controlled exposure to high workload environments, 4) expanded communication requirements, and 5) increased potential for reflective learning. Widespread and effective training in well-replicated, carefully coordinated representations of complex multipatient work environments may strengthen educational interventions for personnel working in high acuity and work-overloaded settings such as the ED. The use of concurrent patient encounter SIM exercises to elicit calculated stressors and to foster compensatory staff behaviors is an educational advance toward this objective. The authors present SIM methodology using concurrent patient encounters to replicate these environments.
The preparation of side chain functionalized analogues of coenzyme Q for protein conjugation studies (2004)

The synthesis of two analogues of CoQ (10 and 13) suitable for conjugation to a peptide or protein, and hence the development of an ELISA immunoassay, is presented. These analogues were synthesized from the protected quinone, 1-bromo-2-methyl-3,4,5,6-tetramethoxybenzene (1), itself prepared from commercially available CoQ-0 (3). Model coupling studies of one of the analogues (10) to N-acetyl-L-lysine methyl ester and a lysine containing dipeptide (N-acetyl-glycine-L-lysine methyl ester) were also undertaken as a first step to monoclonal antibody production.
Mary Strong Clemens, A Botanical Pilgrimage: (2021)

Nelda B. Ikenberry. 2021. Mary Strong Clemens, A Botanical Pilgrimage: Her glorious mission from here to the Outback via Southeast Asia. Sida, Botanical Miscellany 56. (ISSN: 0883-1475; ISBN-13: 978-1-889878-63-8, flex-binding). Botanical Research Institute of Texas Press, 1700 University Dr., Fort Worth, Texas 76107, U.S.A. (Orders: shop.brit.org, orders@brit.org, 1-817-332-4441). $45.00 US, 500 pp., numerous photos, appendix of botanical eponyms, 7 × 10.
Recent Research on Flexible Fixtures for Manufacturing Processes (2013)

Fixtures, are used to fixate, position and support workpieces, and form a crucial tool in manufacturing. Their performance influences the manufacturing (and assembly) process of a product. Furthermore, fixturing can form a significant portion of the needed investment and total process planning time for the manufacturing system. Many fixturing concepts, as contribution to increase the flexibility of the manufacturing system, are reported in the literature. The flexible fixturing designs can be classified into the following seven categories: modular fixtures, flexible pallet systems, sensorbased fixture design, phase-change based concepts, chuck-based concepts, pin-type array fixtures and automatically reconfigurable fixtures. It is observed that the more intelligent and automated fixturing systems are designed with the demands for automation in certain industries in mind. Furthermore, different fixturing solutions suit the engineering demands for different manufacturing areas, this means that for the foreseeable future all technologies will remain current. From the self-reconfigurable fixturing techniques a new fixturing capability is emerging: in process reconfigurability for the optimal placement of clamps and supports during the whole process time. These several concepts together with some recent patents are studied here. The paper concludes with some prospective research directions in the field of flexible fixturing.
Apolipoprotein E expression at neuromuscular junctions in mouse, rat and human skeletal muscle (1994)

Apolipoprotein E (ApoE)-epsilon 4 allele has been associated with late onset familial Alzheimer's disease (AD). In both familial and sporadic AD brain, ApoE is localized to the vessel walls, senile amyloid plaques, and neurofibrillary tangles. ApoE is also an 'injury-response' macromolecule in peripheral nerves and was reported to increase in response to injury. We have demonstrated that Alzheimer beta-amyloid precursor protein and a serpin alpha 1-antichymotrypsin also found accumulated in senile plaques in AD brain, were also localized at neuromuscular junctions (NMJs). Using immunocytochemistry, our present results indicate that ApoE is found in normal mouse, rat and human skeletal muscle and concentrated at the NMJs as it is in the senile plaques in AD brain. Such experiments may shed light on the mechanism of synapse loss, as well as plaque formation in this neurodegenerative disease.
A tunable subwavelength resonant grating optical filter (2003)

A tunable subwavelength resonant grating (SRG) filter with liquid crystal cladding as the tuning layer has been demonstrated for the first time. The filter can be easily fabricated by nanoimprint lithography (NIL). NIL has the advantage of low-cost, high-throughput, and sub-10 nm resolution. The filter has a 17 nm tuning range, which is at least 20 times wider than any previous SRG filter, and 2 nm FWHM. The potential for monolithic integration on a chip, easy fabrication, and low cost will make SRG filter an important element for future WDM optical telecommunication.
Reactive Ion Etching of Silicon and Silicon Dioxide in  CF 4 Plasmas Containing  H 2 or  C 2 F 4 Additives (1991)

Silicon dioxide to silicon reactive ion etch selectivities in plasmas containing either or additives were measured as a function of additive gas concentration. X‐ray photoelectron spectroscopy showed the presence of a fluorocarbon film on etched silicon surfaces that slows the silicon etch rate and is responsible for the selectivities attained in both cases ( etch rate ratio: >30 for , 4–6 for ). The poor selectivity attained using plasmas is due to a significantly lower etch rate relative to the case and is attributed to less energetic ion bombardment of the surface. A slightly higher etch rate of silicon in the plasma was attributed to a higher fluorine concentration in the plasma and a thinner, more fluorinated residue on the surface that allowed etching to continue even at high additive concentrations (>50% additive). The thinner film, despite a higher concentration of polymerizing species in the gas phase, indicates that plasma‐surface interactions play an important role in film formation.
Organizing Publications and Bookmarks in BibSonomy (2007)

BibSonomy is a web-based social resource sharing system which allows users to organise and share bookmarks and publications in a collaborative manner. Apart from standard folksonomy features such as an intuitive user interface, navigation along all dimensions, or browser integration via RSS feeds, BibSonomy provides tag hierarchies, group management and privacy features, and numerous import and export functions.
Galactose Oxidase Model Complexes:  Catalytic Reactivities (1996)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTGalactose Oxidase Model Complexes: Catalytic ReactivitiesYadong Wang and T. D. P. StackView Author Information Department of Chemistry, Stanford University Stanford, California 94305 Cite this: J. Am. Chem. Soc. 1996, 118, 51, 13097–13098Publication Date (Web):December 25, 1996Publication History Received24 June 1996Published online25 December 1996Published inissue 1 January 1996https://pubs.acs.org/doi/10.1021/ja9621354https://doi.org/10.1021/ja9621354rapid-communicationACS PublicationsCopyright © 1996 American Chemical SocietyRequest reuse permissionsArticle Views2097Altmetric-Citations158LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (2)»Supporting Information Supporting Information SUBJECTS:Alcohols,Electron paramagnetic resonance spectroscopy,Ligands,Peptides and proteins,Reactivity Get e-Alerts
A Circuit Modeling Technique for the ISO 7637-3 Capacitive Coupling Clamp Test (2018)

In this paper, we propose a transmission-line modeling technique for the ISO 7637-3 capacitive coupling clamp (CCC) test. Besides modeling the test bench, special attention is devoted to the CCC itself, for which an equivalent circuit is constructed based on the concept of surface transfer impedance and surface transfer admittance. The overall model is validated by means of measurements using a nonlinear circuit as device-under-test, as such demonstrating the appositeness to mimick the CCC test in simulations during the design phase.
Fast Onset of Action of Subcutaneously Administered Marzeptacog Alfa (Activated) Supports on-Demand Treatment in Hemophilia a Mice (2019)

Background: Patients with Hemophilia A or B with and without inhibitors lack treatment options that are fast and easy to use for treating acute bleeding. Currently, their only options are intravenous administration of replacement or bypass therapies. Marzeptacog alfa (activated) (MarzAA) is a novel increased potency recombinant FVIIa in clinical development. We hypothesized that, in addition to its clinically demonstrated efficacy in SC prophylaxis, MarzAA could potentially be used SC on-demand to control acute hemorrhage after bleeding has started. Objective: To demonstrate the procoagulant effect of dosing MarzAA subcutaneously at timepoints both before and after injury using a tail clip bleeding model in hemophilia A mice. Methods: Each mouse was initially weighed and briefly anesthetized with isoflurane for collection of 5 µL blood to assess baseline hemoglobin levels. Later, anaesthetized (100 mg/kg ketamine + 10 mg/kg xylazine) F8-/- (Haem A, B6;129S4-F8tm1Kaz/J) mice were submitted to a tail clip injury model completely transecting the tail at a diameter of 1.25 mm from the tip using a sharp razor blade. Following injury, blood loss was monitored with the tail submerged in warm saline for 20 minutes and quantified by hemoglobin content. Mice were dosed with MarzAA SC (1.5 mg/kg) or vehicle at 15, 30, 60, or 90 minutes before or one minute after injury (1.5 and 4.5 mg/kg). An ED50 for MarzAA was determined using various doses ranging from 0.15 mg/kg to 3.0 mg/kg. Statistical significance was set at α = 0.05. Results: SC administration of MarzAA resulted in a dose dependent reduction in bleeding when administered to hemophilia A mice 15 minutes before injury. At the highest dose of MarzAA, full efficacy was achieved as bleeding in the treated mice was comparable to the blood loss observed in normal hemostatically competent mice. The ED50 for MarzAA was calculated to be 387 µg/kg after SC administration in this model. More importantly, when dosed as a rescue therapy one minute after injury, SC MarzAA significantly reduced bleeding to 350±46 µL from 635±50 µL (vehicle), p = 0.02. Conclusion: MarzAA demonstrated efficacy when administered SC both before and after injury. Taken together these data suggest that SC MarzAA could potentially be used on-demand to treat patients with acute bleeding. These results provide a basis for further clinical investigation of the acute treatment of a bleed with SC MarzAA in hemophilia patients. Disclosures Del Greco: Catalyst Biosciences: Consultancy, Equity Ownership. Neuman:Catalyst Biosciences: Employment, Equity Ownership. Levy:Catalyst Biosciences: Employment, Equity Ownership. Reckless:RxCelerate: Employment. Knudsen:Catalyst Biosciences: Employment, Equity Ownership. Blouse:Catalyst Biosciences: Employment, Equity Ownership.
Expansion of repertoire of modified DNAs prepared by PCR using KOD Dash DNA polymerase (2005)

Thymidine analogues bearing a variety of functional groups at the C5-position via an amino-linker arm were prepared and the substrate activity for PCR using thermophilic KOD Dash DNA polymerase was examined. The enzyme accepted the thymidine analogues bearing pyridine, imidazole, biotin, a cationic-charged guanidinium, a cationic-charged amino, mercaptopyridyl and phenanthrolne groups at the C5-position, forming the corresponding PCR product. However, a thymidine analogue bearing a carboxyl group at the C5-position was a poor substrate and the corresponding PCR products could not be obtained. The thymidine analogue bearing a mercapto group was also a poor substrate for the enzyme, because it dimerized by disulfide linkage under PCR conditions. The enzyme hardly accepts the thymidine analogues with a negatively-charged carboxyl group or a bulky group as a substrate. KOD Dash DNA polymerase, having a broader substrate specificity than any other DNA polymerase, will expand the variety of modified DNAs that can be prepared by PCR.
NASA SCEPTOR electric concept aircraft power system: X-plane electric propulsion system design and qualification for crewed flight testing (2016)

We're at the beginning of a 30–50 year propulsion revolution Electric propulsion is not merely about propulsion, it's about being able to apply a scale-free technology to fundamentally change how we design vehicles Synergistic integration of Distributed Electric Propulsion will transform aircraft, and the missions they perform, and potentially society The age of on-demand services is about to lead to On-Demand Mobility
Role of Dispersion and Electrostatic Forces on Solute−Solvent Interactions in a Nematic Liquid Crystal Phase (1998)

A series of 23 substituted biphenyl, phenylpyridine, and bipyridine derivatives were doped in the nematic liquid crystal host 4‘-(pentyloxy)-4-biphenylcarbonitrile over the mole fraction range 0.005 ≤ xd ≤ 0.04. The effect of each biaryl dopant on the nematic−isotropic phase transition temperature (clearing point) of the liquid crystal phase was determined by differential scanning calorimetry as a function of dopant mole fraction for a wide range of substituents and expressed as a constant of proportionality δI. The molecular polarizability (α) and electrostatic character (EHOMO) of each dopant were estimated using ab initio calculations at the HF/3-21G* level. Analysis of the combined δI data with respect to α and EHOMO by multilinear regression showed that a reasonable correlation exists between δI and a linear combination of α and EHOMO (R2 = 0.81) which represents a statistically significant improvement over correlations between δI and α (R2 = 0.72) and between δI and EHOMO (R2 = 0.56). These results provide evidence that arene−arene interactions in the absence of a ternary solvent are controlled by both electrostatic and dispersion forces. In this work, dispersion forces were shown to strongly influence the stability of the nematic phase, with a secondary influence coming from electrostatic forces. Furthermore, the results suggest that charge-transfer complexation and molecular dipole−dipole interactions do not have an appreciable effect on the stability of arene−arene interactions in the nematic phase.
Osteosarcoma: a review of current and future therapeutic approaches (2021)

Osteosarcoma (OS) is the most common primary bone malignancy that affects children and young adults. OS is characterized by a high degree of malignancy, strong invasiveness, rapid disease progression, and extremely high mortality rate; it is considered as a serious threat to the human health globally. The incidence of OS is common in the metaphysis of long tubular bones, but rare in the spine, pelvis, and sacrum areas; moreover, majority of the OS patients present with only a single lesion. OS has a bimodal distribution pattern, that is, its incidence peaks in the second decade of life and in late adulthood. We examine historical and current literature to present a succinct review of OS. In this review, we have discussed the types, clinical diagnosis, and modern and future treatment methods of OS. The purpose of this article is to inspire new ideas to develop more effective therapeutic options.
Retargeted Oncolytic Measles Strains Entering via the EGFRvIII Receptor Maintain Significant Antitumor Activity against Gliomas with Increased Tumor Specificity (2006)

Among the best-characterized genetic alterations in gliomas is the amplification of the epidermal growth factor receptor (EGFR) gene, present in approximately 40% of glioblastoma multiforme, and frequently associated with the EGFRvIII gene rearrangement. We have previously shown that attenuated vaccine strains of measles virus have potent antitumor activity against gliomas, and identified H protein mutations, which ablate recognition of the natural measles virus receptors CD46 and SLAM. Retargeted recombinant viruses were generated from the measles Edmonston-NSe vaccine strain displaying a single-chain antibody against EGFRvIII at the COOH terminus of H and containing the marker green fluorescent protein (GFP) gene in position 1. Two different H mutants were employed: H(SNS) (V451S, Y481N, and A527S)-CD46 blind, and H(AA) (Y481A and R533A)-CD46 and SLAM blind. MV-GFP virus was used as a positive control. Both EGFRvIII-retargeted viruses had significant antitumor activity against EGFRvIII-expressing glioblastoma multiforme but no cytopathic effect against normal cells. In an orthotopic model of EGFRvIII-expressing GBM39 xenografts, there was comparable therapeutic efficacy between retargeted strains and unmodified MV-GFP and statistically significant prolongation of survival in treated animals compared with the control group (P = 0.001). Formation of syncytia was observed in tumors treated with retargeted viruses, with a surrounding infiltrate consisting of macrophages and natural killer cells. In summary, EGFRvIII-retargeted oncolytic measles virus strains have comparable therapeutic efficacy with the unmodified MV-GFP strain against EGFRvIII-expressing glioma lines and xenografts with improved therapeutic index, a finding with potential translational implications in glioma virotherapy.