gebhart/patrepeval huggingface dataset:
Droplet-based magnetically activated cell separation (2005)

In this study, we developed a method that target cells in suspension can be separated by combining magnetic force and gravitation force. Since the newly developed method involves a separating process of a droplet containing nontarget cells in suspension by applying magnetic force to separate target cells, we called it droplet-based magnetic activated cell sorting (dMACS). To demonstrate the efficiency of the dMACS system, Ter119 (+) cells from mouse bone marrow cells were separated by both conventional MACS and our dMACS systems. Effects of three parameters on separation efficiency were examined in the dMACS system. As a result, both volume of droplet of cell suspension, and magnetic force did not affect the efficiency of cell separation markedly. However, the time for cell settlement in the droplet showed a critical role in the efficiency of cell separation according to increasing time. Therefore, we tried to verify that the saturation time affected increase of its efficiency and that flow rate injected to get rid of the negative cell resulted in the decrease of its efficiency. Using this dMACS system, we were able to pinpoint that the flow rate of cell suspension injected into a magnetic platform results in disturbance in the droplet, leading to turbulence in the cell suspension.
DeepPredict: A deep predictive intelligence platform for patient monitoring (2017)

A novel platform, DeepPredict, for predicting hospital bed exit events from video camera systems is proposed. DeepPredict processes video data with a deep convolutional neural network consisting of five main layers: a 1 × 1 3D convolutional layer used for generating feature maps from raw video data, a context-aware pooling layer used for rectifying data from different camera angles, two fully connected layers used for applying pre-trained deep features, and an output layer used to provide a likelihood of a bed exit event. Results for a model trained on 180 hours of data demonstrate accuracy, sensitivity, and specificity of 86.47%, 78.87%, and 94.07%, respectively, when predicting a bed exit event up to seven seconds in advance.
Mechanism-based sirtuin enzyme activation (2015)

Abstract Sirtuin enzymes are NAD + -dependent protein deacylases that play a central role in the regulation of healthspan and lifespan in organisms ranging from yeast to mammals. There is intense interest in the activation of the seven mammalian sirtuins (SIRT1-7) in order to extend mammalian healthspan and lifespan. However, there is currently no understanding of how to design sirtuin-activating compounds beyond allosteric activators of SIRT1-catalyzed reactions that are limited to particular substrates. Moreover, across all families of enzymes, only a dozen or so distinct classes of non-natural small molecule activators have been characterized, with only four known modes of activation among them. None of these modes of activation are based on the unique catalytic reaction mechanisms of the target enzymes. Here, we report a general mode of sirtuin activation that is distinct from the known modes of enzyme activation. Based on the conserved mechanism of sirtuin-catalyzed deacylation reactions, we establish biophysical properties of small molecule modulators that can in principle result in enzyme activation for diverse sirtuins and substrates. Building upon this framework, we propose strategies for the identification, characterization and evolution of hits for mechanism-based enzyme activating compounds. We characterize several small molecules reported in the literature to activate sirtuins besides SIRT1, using a variety of biochemical and biophysical techniques including label-free and labeled kinetic and thermodynamic assays with multiple substrates and protocols for the identification of false positives. We provide evidence indicating that several of these small molecules reported in the published literature are false positives, and identify others as hit compounds for the design of compounds that can activate sirtuins through the proposed mechanism-based mode of action.
Novel Preparation Method of Hydroxyapatite Fibers (1998)

A novel method for preparing calcium hydroxyapatite (Ca 10 (PO 4 ) 6 (OH) 2 : HAp) fibers has been developed. HAp fibers can be prepared successfully by heating a compact consisting of calcium metaphosphate (ß‐Ca(PO 3 ) 2 ) fibers with Ca(OH) 2 particles in air at 1000°C and subsequently treating the resultant compact with dilute aqueous HCl solution. The ß‐Ca(PO 3 ) 2 fibers and the Ca(OH) 2 in the compact were converted into fibrous HAp and CaO phases by the heating, and the CaO phase was removed by acid‐leaching. HAp fibers obtained in the present work were 40‐150 µm in length and 2‐10 µm in diameter. The fibers had almost the same dimensions as those of the ß‐Ca(PO 3 ) 2 fibers.
Dynamic Evolution of Business Performance Management (2006)

Business performance management (BPM) is a new frontier in IT-enabled enterprise that supports the monitoring business operations. BPM solutions must be able to efficiently process business events, compute business metrics, detect business situations, and provide the real-time visibility of key performance indicators (KPIs) in dynamic environments, wherein sources of dynamicity are plentiful, including new strategies, operations, KPIs, etc. Therefore, BPM solutions need to adapt these changes by dynamic evolution. We propose a policy-driven approach, where evolution policies capture the evolution mechanism of BPM solutions. This frees solution developers from low-level programming concerns. We implemented a hybrid compilation-interpretation framework that enables execution of wide spectrums of evolution policies. Further, our framework enables execution of evolution policies in parallel with on going event processing and guarantees the integrity on both of them
A codec for HDTV (1992)

A high-quality digital video codec has been developed for the Zenith/AT&T HDTV system. It adaptively selects between two transmission modes with differing rates and robustness. The codec works on an image progressively scanned with 1575 scan lines every 1/30th of a second and achieves a compression ratio of approximately 50 to 1. The high compression ratio facilitates robust transmission of the compressed HDTV signal within an NTSC taboo channel. Transparent image quality is achieved using motion compensated transform coding coupled with a perceptual criterion to determine the quantization accuracy required for each transform coefficient. The codec has been designed to minimize complexity and memory in the receiver.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Ultra-low-power circuits (2010)

An ultra-low-power transconductance device is provided, (FIG. 1b, FIG. 1c), comprising a series connection of a transistor of a first channel type (A) and a transistor of a second channel type (B), the first channel type having a different polarity than the second channel type. The transistors each have a source, a drain and a gate. The source of the transistor of the first channel type (A) is coupled with the source of the transistor of the second channel type (B) and the drain of the transistor of the first channel type (A) is coupled with the gate of the transistor of the second channel type (B).
Circular Saw Vibration Control by Induction of Thermal Membrane Stresses (1981)

Circular saw transverse vibration and product dimensional accuracy were measured during a series of production experiments in which sawblade vibration was controlled by the deliberate introduction of thermal membrane stresses. Induction heating near the saw collar was used to control the temperature difference between two concentric annular zones on the sawblade surface. Optimal temperature conditions were predicted using the critical speed stability theory for symmetrical circular saws and these predictions were verified through production experiments. Feedback control of sawblade temperature was successfully demonstrated in production as a means of reducing blade vibration and improving dimensional accuracy.
Computer-aided detection of masses in digital tomosynthesis mammography: combination of 3D and 2D detection information (2007)

We are developing a computer-aided detection (CAD) system for masses on digital breast tomosynthesis mammograms (DBTs). The CAD system includes two parallel processes. In the first process, mass detection and feature analysis are performed in the reconstructed 3D DBT volume. A mass likelihood score is estimated for each mass candidate using a linear discriminant (LDA) classifier. In the second process, mass detection and feature analysis are applied to the individual projection view (PV) images. A mass likelihood score is estimated for each mass candidate using another LDA classifier. The mass likelihood images derived from the PVs are back-projected to the breast volume to estimate the 3D spatial distribution of the mass likelihood scores. The mass likelihood scores estimated by the two processes at the corresponding 3D location are then merged and evaluated using FROC analysis. In this preliminary study, a data set of 52 DBT cases acquired with a GE prototype system at the Massachusetts General Hospital was used. The LDA classifiers with stepwise feature selection were designed with leave-one-case-out resampling. In an FROC analysis, the CAD system for detection in the DBT volume alone achieved test sensitivities of 80% and 90% at an average FP rate of 1.6 and 3.0 per breast, respectively. In comparison, the average FP rates of the combined system were 1.2 and 2.3 per breast, respectively, at the same sensitivities. The combined system is a promising approach to improving mass detection on DBTs.
Double-Jet Ejection of Cooling Air for Improved Film-Cooling (2006)

Film-cooling in gas turbines leads to aerodynamic mixing losses and reduced temperatures of the gas flow. Improvements of the gas turbine thermal efficiency can be achieved by reducing the cooling fluid amount and by establishing a more equal distribution of the cooling fluid along the surface. It is well known that vortex systems in the cooling jets are the origin of reduced film-cooling effectiveness. For the streamwise ejection case, kidney-vortices result in a lift-off of the cooling jets; for the lateral ejection case, usually only one dominating vortex remains, leading to hot gas flow underneath the jet from one side. Based on the results of numerical analyses, a new cooling technology has been introduced by the authors, which reaches high film-cooling effectiveness as a result of a well-designed cooling hole arrangement for interaction of two neighbouring cooling jets (Double-jet Film-cooling DJFC). The results show that configurations exist, where an improved film-cooling effectiveness can be reached because an anti-kidney vortex pair is established in the double-jet. The paper aims on following major contributions: • to introduce the Double-jet Film-cooling (DJFC) as an alternative film-cooling technology to conventional film-cooling design. • to explain the major phenomena, which lead to the improvement of the film-cooling effectiveness by application of the DJFC. • to prove basic applicability of the DJFC to a realistic blade cooling configuration and present first test results under machine operating conditions.
Bit error rate of TH-BPSK UWB receivers in multiuser interference (2009)

It is well known that the Gaussian distribution is not an accurate model for approximating the probability density function (PDF) of the multiple access interference (MAI) in timehopping UWB (TH-UWB) systems. An exact theoretical model which explains the key features of the PDF of the MAI in THUWB systems is discussed. These features, which can be precisely anticipated by the proposed model include impulses, singularities, and the tail behaviour in the distribution of the MAI. The model reveals in quantitative terms why a Gaussian approximation for the MAI in TH-UWB systems is highly imprecise even in an environment with a large number of independent interferers. Based on the model obtained for the PDF of the MAI and exploiting the maximum a posteriori (MAP) receiver design rule, the optimal attainable BER performance of binary TH-UWB receivers in additive white Gaussian noise channels is numerically determined. The performances of some recently proposed UWB receivers are benchmarked against the optimal performance showing that some of them achieve near-optimal performance.
Synchronous/asynchronous independent single sideband acoustic telemetry (1984)

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Excision of HIV-1 Proviral DNA by Recombinant Cell Permeable Tre-Recombinase (2012)

Over the previous years, comprehensive studies on antiretroviral drugs resulted in the successful introduction of highly active antiretroviral therapy (HAART) into clinical practice for treatment of HIV/AIDS. However, there is still need for new therapeutic approaches, since HAART cannot eradicate HIV-1 from the infected organism and, unfortunately, can be associated with long-term toxicity and the development of drug resistance. In contrast, novel gene therapy strategies may have the potential to reverse the infection by eradicating HIV-1. For example, expression of long terminal repeat (LTR)-specific recombinase (Tre-recombinase) has been shown to result in chromosomal excision of proviral DNA and, in consequence, in the eradication of HIV-1 from infected cell cultures. However, the delivery of Tre-recombinase currently depends on the genetic manipulation of target cells, a process that is complicating such therapeutic approaches and, thus, might be undesirable in a clinical setting. In this report we demonstrate that E.coli expressed Tre-recombinases, tagged either with the protein transduction domain (PTD) from the HIV-1 Tat trans-activator or the translocation motif (TLM) of the Hepatitis B virus PreS2 protein, were able to translocate efficiently into cells and showed significant recombination activity on HIV-1 LTR sequences. Tre activity was observed using episomal and stable integrated reporter constructs in transfected HeLa cells. Furthermore, the TLM-tagged enzyme was able to excise the full-length proviral DNA from chromosomal integration sites of HIV-1-infected HeLa and CEM-SS cells. The presented data confirm Tre-recombinase activity on integrated HIV-1 and provide the basis for the non-genetic transient application of engineered recombinases, which may be a valuable component of future HIV eradication strategies.
Compact Optical Correlator (1993)

Optical cross-correlator indicates position of target (if any) in scene matching target encoded in matched filter. Size reduced by use of prisms to fold optical path. Device designed, assembled from commercial components, and tested to verify feasibility of miniaturization for eventual incorporation into navigational and/or control systems of autonomous vehicles. For example, aircraft equipped with optical correlator recognizes prescribed target and measures its position, so that it lands on target under automatic control.
Small-molecule cyclic models for the synthesis of new polyphosphazenes: side-group construction via lithiophenoxy derivatives (1980)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSmall-molecule cyclic models for the synthesis of new polyphosphazenes: side-group construction via lithiophenoxy derivativesH. R. Allcock, T. L. Evans, and T. J. FullerCite this: Inorg. Chem. 1980, 19, 4, 1026–1030Publication Date (Print):April 1, 1980Publication History Published online1 May 2002Published inissue 1 April 1980https://pubs.acs.org/doi/10.1021/ic50206a047https://doi.org/10.1021/ic50206a047research-articleACS PublicationsRequest reuse permissionsArticle Views178Altmetric-Citations33LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Single cysteine substitution in <i>Bacillus thuringiensis</i> Cry7Ba1 improves the crystal solubility and produces toxicity to <i>Plutella xylostella</i> larvae (2011)

Many Bacillus thuringiensis isolates have no demonstrated toxicity against insects. In this study, a novel holotype crystal protein gene cry7Ba1 was isolated from a 'non-insecticidal'B. thuringiensis strain YBT-978. The Cry7Ba1 protein showed high toxicity against Plutella xylostella larvae after the crystals were dissolved at pH 12.5, suggesting that the 'non-insecticidal' properties of this protein were due to insolubility in the normal insect midgut pH environment. After the C-terminal half of Cry7Ba1 was replaced by that of Cry1Ac or Cry1C proteins, the recombinant protein inclusions could be dissolved at pH 9.5, and exhibited high toxicity against P. xylostella larvae. This result proved the insolubility of Cry7Ba1 crystal was determined by the structure of its C-terminal half. Further, six mutations were constructed by substituting cysteine residues with serine. Solubility studies showed that the crystals from mutants C697S, C834S and C854S could be dissolved at lower pH (10.5, 9.5 and 11.5 respectively). Bioassays showed that crystals from mutant C834S were toxic to P. xylostella larvae. Our discoveries suggest that a single cysteine residue located in the C-terminal half of the protein determines the solubility and toxicity of some nontoxic crystal proteins. This study provides a strategy to isolate novel insecticidal crystal protein genes from 'non-insecticidal'B. thuringiensis strains.
All-Diamond Microelectrode Array Device (2005)

We report the development of all-diamond microelectrochemical devices, namely, a microelectrode array (MEA), in which a periodic array structure with well-defined diameters, distance, and hexagonal unit cell pattern is micromachined using a combination of state-of-the-art microwave-induced plasma growth and laser ablation shaping techniques to prepare and coat a patterned boron-doped diamond (BDD) substrate with an intrinsic diamond insulating layer. The active BDD element can be tuned to between 10 and 50 microm in diameter with a 10 times diameter center-to-center distance between two adjacent conducting elements, which are exactly coplanar to the dielectric surroundings. This type of device should enable applications in harsh conditions such as high temperature, high pressure, and resistive media under dynamic flow regimes.
Biosynthesis of the Pharmaceutically Important Fungal Ergot Alkaloid Dihydrolysergic Acid Requires a Specialized Allele of <i>cloA</i> (2017)

Ergot alkaloids are specialized fungal metabolites that are important as the bases of several pharmaceuticals. Many ergot alkaloids are derivatives of lysergic acid (LA) and have vasoconstrictive activity, whereas several dihydrolysergic acid (DHLA) derivatives are vasorelaxant. The pathway to LA is established, with the P450 monooxygenase CloA playing a key role in oxidizing its substrate agroclavine to LA. We analyzed the activities of products of <i>cloA</i> alleles from different fungi relative to DHLA biosynthesis by expressing them in a mutant of the fungus <i>Neosartorya fumigata</i> that accumulates festuclavine, the precursor to DHLA. Transformants expressing CloA from <i>Epichloë typhina</i> × <i>Epichloë festucae</i>, which oxidizes agroclavine to LA, failed to oxidize festuclavine to DHLA. In substrate feeding experiments, these same transformants oxidized exogenously supplied agroclavine to LA, indicating that a functional CloA was produced. A genomic clone of <i>cloA</i> from <i>Claviceps africana</i>, a sorghum ergot fungus that produces a DHLA derivative, was cloned and expressed in the festuclavine-accumulating mutant of <i>N. fumigata</i>, but several introns in this genomic clone were not processed properly. Expression of a synthetic intron-free version of <i>C. africana</i><i>cloA</i> resulted in the accumulation of DHLA as assessed by fluorescence high-pressure liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS). In substrate feeding experiments, the <i>C. africana</i> CloA also accepted agroclavine as the substrate, oxidizing it to LA. The data indicate that a specialized allele of <i>cloA</i> is required for DHLA biosynthesis and that the pharmaceutically important compound DHLA can be produced in engineered <i>N. fumigata</i><b>IMPORTANCE</b> Ergot alkaloids are fungal metabolites that have impacted humankind historically as poisons and more recently as pharmaceuticals used to treat dementia, migraines, and other disorders. Much is known about the biosynthesis of ergot alkaloids that are derived from lysergic acid (LA), but important questions remain about a parallel pathway to ergot alkaloids derived from dihydrolysergic acid (DHLA). DHLA-derived alkaloids have minor structural differences compared to LA-derived alkaloids but can have very different activities. To understand how DHLA is made, we analyzed activities of a key enzyme in the DHLA pathway and found that it differed from its counterpart in the LA pathway. Our data indicate a critical difference between the two pathways and provide a strategy for producing DHLA by modifying a model fungus. The ability to produce DHLA in a model fungus may facilitate synthesis of DHLA-derived pharmaceuticals.
Atypical Leber's Hereditary Optic Neuropathy With Molecular Confirmation (1993)

The diagnosis of LHON should be considered in all cases of unexplained optic neuropathy, including those with negative family history, late or early age at onset, female gender, or normal funduscopic appearance.
Multiport power divider-combiner circuits using circular-sector-shaped planar components (1988)

A sector-shaped configuration for designing planar power divider-combiner circuits that are compatible with microstrip circuits is discussed. This configuration is analyzed using the two-dimensional planar circuit approach. Expressions for impedance matrix parameters of multiport sectorial planar circuits are given. Theoretical and experimental results reported for 90 degrees and 180 degrees power dividers are in good agreement.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>