gebhart/patrepeval huggingface dataset:
Human Red Blood Cells Contain Antifibrogenic Factors That Stimulate MMP-1 Expression in Dermal Fibroblasts (2012)

The healing of a burn injury is a dynamic biological process, and there must be a set of factors that gradually slow down and/or terminate this healing process upon epithelialization. We have previously demonstrated that some members of the 14-3-3 protein family including stratifin, which is mainly released by differentiated keratinocytes, have a potent matrix metalloproteinase-1 (MMP-1), -3, -8, and -24 stimulatory effect for fibroblasts. During the course of our studies, we discovered that human red blood cells (RBCs) contain a high level of different 14-3-3 isoforms. In this study, we evaluated the presence of these proteins in RBC and examined the efficacy of RBC in stimulating the expression of MMP-1 in dermal fibroblasts using Western blot analysis. The findings revealed the presence of all seven isoforms of 14-3-3 (η, γ, ε, σ, β, ζ, and τ) in RBC lysate, though with different levels of expression. Interestingly, the levels of some of these proteins were higher in RBC from new born babies compared to those of adults. We also found that RBC lysate significantly increased the expression of MMP-1 in different strains of fibroblasts, and this effect has been abrogated by almost 20% when R18 was used to deplete the 14-3-3 proteins from lysate. However, when RBC lysate was passed through a 3-kDa filter, and then whole RBC lysate, retentate, and filtrate were used to treat three strains of fibroblasts, MMP-1 expression was increased significantly, indicating the presence of MMP-1-inducing factors smaller than 3kDa in RBC lysate as well. The MMP-1 stimulatory effect of RBC lysate for fibroblasts was stable at freeze/thawing as well as freezing for at least 1 year. In conclusion, the MMP-1 stimulatory effect of RBC lysates might play an important role in the regulation of extracellular matrix under physiological and pathological conditions. Because of its MMP-1 stimulating effect, the RBC lysate might be used as an antifibrogenic factor for the treatment of some fibroproliferative conditions such as hypertrophic scarring and keloid.
Trial Watch—Oncolytic viruses and cancer therapy (2015)

Oncolytic virotherapy relies on the administration of non-pathogenic viral strains that selectively infect and kill malignant cells while favoring the elicitation of a therapeutically relevant tumor-targeting immune response. During the past few years, great efforts have been dedicated to the development of oncolytic viruses with improved specificity and potency. Such an intense wave of investigation has culminated this year in the regulatory approval by the US Food and Drug Administration (FDA) of a genetically engineered oncolytic viral strain for use in melanoma patients. Here, we summarize recent preclinical and clinical advances in oncolytic virotherapy.
Palladium-Catalyzed Enantioselective Diboration of Prochiral Allenes (2004)

Pd-catalyzed diboration of prochiral allenes occurs exclusively at the internal position and is remarkably accelerated in the presence of Lewis basic ligand structures. On the basis of preliminary observations, a chiral ligand was employed, and the enantiomeric excess of a variety of diboration products was found to be in the range of 86-92% ee. The chiral diboron reaction products should be useful in organic synthesis, and preliminary experiments suggest that they may participate in allylation reactions with a high level of chirality transfer.
Molecular analysis of pleckstrin: The major protein kinase c substrate of platelets (1989)

Activation of protein kinase C (PKC) in platelets causes the immediate phosphorylation of pleckstrin, an apparent Mr 40-47,000 protein previously called 40K or P47. Pleckstrin presumably plays an important but as yet unknown role in mediating cellular responses evoked by agonist-induced phosphoinositide turnover. We have cloned the cDNA for pleckstrin from the HL-60 human promyelocytic leukemia cell line by immunological screening of a lambda gt11 expression library (Tyers et al.: Nature 333:470-473, 1988) and now report further analysis of the pleckstrin sequence. Pleckstrin has a deduced Mr of 40,087 and is encoded by a 1,050-bp open reading frame which is preceded by a short open reading frame that terminates before the correct initiator methionine. A single polymorphic site was found in the coding region. An unusual pattern of sequence heterogeneity occurred about a poly(A) tract in the 3' untranslated region. The 3.0-kb pleckstrin mRNA induced upon differentiation of HL-60 cells apparently has heterogeneous 5' ends which undergo differential regulation during HL-60 cell maturation. Analysis by multiple sequence alignment with known PKC substrates identified a strong candidate site for phosphorylation by PKC and a potential Ca2+-binding EF-hand motif. No other similarities to proteins in current databases were found.
Airblast Attenuation and Flow Loss Performance of Passive Attenuators (1992)

Abstract : An analytical/ experimental program was conducted for Waterways Experiment Station (WES) to develop a passive airblast attenuator to protect hardened shelters from conventional weapon attack. The role of a passive attenuator is to provide protection through attenuation of air shock, which would otherwise enter air ducts, damaging sensitive air filtration and decontamination equipment, electronic and mechanical systems, and injuring building occupants. The design constraints placed on the passive attenuator were that it have no moving parts, be inexpensive, and require no maintenance. Further, air flow loss under normal operating conditions was not to exceed 2.0 in.-H(2)O at 500 ft/min. The goal was 99% attenuation of both pressure and impulse. A Phase I analytical study evaluated the feasibility of using Suppressive Shields concepts for passive attenuators. A series of orifice plates was found to offer less resistance to normal air flow, yet equal or exceed the attenuation levels of any of the Suppressive Shields concepts. The Phase II experimental work included both airblast attenuation and flow loss measurements, and evaluated six concepts, three of which were orifice plate designs. The flow loss requirement proved to be very stringent, and measured losses were greater than analytical predictions. Airblast attenuation levels of 86 to 92% were measured with several orifice plate designs, but with 10 to 14 in.-H(2)O pressure drop. Two orifice plate concepts achieved 80 to 85% attenuation of both pressure and impulse at the 2.4 in.-H(2)O requirement. Departing from orifice plate designs in an attempt to improve performance, three concepts were evaluated that directed the shock wave into holding chambers. The best of these designs achieved 87-9596 attenuation, with a flow loss of 2.6 in.-H(2)O at 500 ft/min.
Design and experimental demonstration of Doppler cloak from spatiotemporally modulated metamaterials based on rotational Doppler effect (2020)

Recently, spatiotemporally modulated metamaterial has been theoretically demonstrated for the design of Doppler cloak, a technique used to cloak the motion of moving objects from the observer by compensating for the Doppler shift. Linear Doppler effect has an angular counterpart, i.e., the rotational Doppler effect, which can be observed by the orbital angular momentum (OAM) of light scattered from a spinning object. In this work, we predict that the spatiotemporally modulated metamaterial has its angular equivalent phenomenon. We therefore propose a technique to observe the rotational Doppler effect by cylindrical spatiotemporally modulated metamaterial. Conversely, such a metamaterial is able to cloak the Doppler shift associated with linear motion by generating an opposite rotational Doppler shift. This novel concept is theoretically analyzed, and a conceptual design by spatiotemporally modulating the permittivity of a voltage-controlled OAM ferroelectric reflector is demonstrated by theoretical calculation and numerical simulation. Finally, a Doppler cloak is experimentally demonstrated by a spinning OAM metasurface in radar system, which the spatiotemporal reflection phase are mechanically modulated. Our work presented in this paper may pave the way for new directions of OAM carrying beams and science of cloaking, and also explore the potential applications of tunable materials and metasurfaces.
Treatment of Attention-Deficit/Hyperactivity Disorder: Overview of the Evidence (2005)

The American Academy of Pediatrics' Committee on Quality Improvement, Subcommittee on Attention-Deficit/Hyperactivity Disorder, reviewed and analyzed the current literature for the purpose of developing an evidence-based clinical practice guideline for the treatment of the school-aged child with attention-deficit/hyperactivity disorder (ADHD). This review included several key reports, including an evidence review from the McMaster Evidence-Based Practice Center (supported by the Agency for Healthcare Research and Quality), a report from the Canadian Coordinating Office for Health Technology Assessment, the Multimodal Treatment for ADHD comparative clinical trial (supported by the National Institute of Mental Health), and supplemental reviews conducted by the subcommittee. These reviews provided substantial information about different treatments for ADHD and their efficacy in improving certain characteristics or outcomes for children with ADHD as well as adverse effects and benefits of multiple modes of treatment compared with single modes (eg, medication or behavior therapies alone). The reviews also compared the effects of different medications. Other evidence documents the long-term nature of ADHD in children and its classification as a chronic condition, meriting the application of general concepts of chronic-condition management, including an individual treatment plan with a focus on ongoing parent and child education, management, and monitoring. The evidence strongly supports the use of stimulant medications for treating the core symptoms of children with ADHD and, to a lesser degree, for improving functioning. Behavior therapy alone has only limited effect on symptoms or functioning of children with ADHD, although combining behavior therapy with medication seems to improve functioning and may decrease the amount of (stimulant) medication needed. Comparison among stimulants (mainly methylphenidate and amphetamines) did not indicate that 1 class outperformed the other.
Kinetics and modelling of furfural oxidation with hydrogen peroxide over a fibrous heterogeneous catalyst: effect of reaction parameters on yields of succinic acid (2017)

Abstract BACKGROUND Succinic acid is an important intermediate compound with high commercial significance in the chemical industry. It can be obtained from renewable resources such as furfural. This article describes the kinetic study of furfural oxidation with hydrogen peroxide over a fibrous polymer‐supported sulphonic acid catalyst (Smopex‐101). The influence of reaction temperature, initial concentration of hydrogen peroxide and furfural has been investigated in detail. Reaction kinetics has been studied both in batch and semi‐batch modes. Self‐decomposition of hydrogen peroxide and loss of carbon in the form of carbon dioxide has been thoroughly examined. Parameter estimation has been performed and comparison was made between catalytic and non‐catalytic reactions. The catalyst stabilities have been discussed. RESULTS The results indicate that the reaction orders in hydrogen peroxide decomposition are different in catalytic and non‐catalytic reactions. Furfural presence in the reaction mixture, its interactions with the catalyst and involvement in other reactions significantly suppressed the side reactions, improving hydrogen peroxide utilization. The desired product (succinic acid) has been obtained in good yields (80%) when furfural was added in a semi‐batch mode. CONCLUSIONS The kinetic models predicted the consumption behavior of hydrogen peroxide and furfural in both catalytic and non‐catalytic reactions and the kinetic parameters were statistically well identified. © 2017 Society of Chemical Industry
Formulation Effects on Ocular Absorption of Brimonidine in Rabbit Eyes (2002)

Purite (stabilized oxychloro complex) and benzalkonium chloride (BAK) are preservatives. We investigated formulation effects on ocular absorption of brimonidine in rabbit eyes. The formulations compared were: Alphagan (0.2% brimonidine tartrate/0.005% BAK, pH 6.4), Brimonidine-Purite (0.2% brimonidine tartrate/0.005% Purite, pH 7.2), and Brimonidine-PF (0.2% brimonidine tartrate, preservative-free (PF), pH 6.4) solutions. The study was conducted in a cross-over fashion; albino rabbits (n = 18) were given a single 35 microl drop of each test formulation in each eye. Aqueous humor samples were collected at selected times post-dose from subgroups of 2 rabbits per timepoint and analyzed for brimonidine concentrations by LC-MS/MS. The AUC and Cmax were calculated. The results showed rapid ocular absorption of brimonidine, with peak concentrations at 0.33-1 hr. The AUC(0-5hr) values were 3.78 +/- 0.38, 2.77 +/- 0.22, and 2.49 +/- 0.22 microg-hr/ml (mean +/- SEM) for Brimonidine-Purite, Alphagan and Brimonidine-PF, respectively. The corresponding Cmax values were 2.69 +/- 0.72, 1.74 +/- 0.13, and 1.24 +/- 0.22 microg/ml (mean +/- SEM). Brimonidine-Purite provided significantly higher AUC(0-5hr) than Alphagan (p < 0.05). No statistical significant difference in AUC(0-5hr) was found between Alphagan and Brimonidine-PF. In conclusion, 0.2% Brimonidine-Purite was 1.4 and 1.5 times more ocularly bioavailable in rabbits than 0.2% Alphagan and 0.2% Brimonidine-PF, respectively.
Extraction of DNA from sheep white blood cells (1990)

Abstract Common methods for the extraction of DNA from whole blood involve several phenol extraction steps. Phenol is corrosive and toxic, and the extraction steps are time‐consuming, limiting the number of samples that can be processed. Two other methods using high salt or guanidine hydrochloride were tested for extraction of DNA from sheep blood. Extraction using the guanidine hydrochloride method resulted in a gelatinous material that failed to resuspend in TE buffer. The high salt method produced a good yield of high molecular weight DNA as determined by agarose gel electrophoresis. The mean yields of DNA from 100 samples of 20 ml of whole blood extracted by either the phenol or the high salt methods were 0.50 mg (SD=0.19) and 0.64 mg (SD=0.26) respectively. The quality of the DNA extracted by the high salt method was equivalent to that extracted by the phenol method and the DNA from both methods was digested to completion with a range of restriction enzymes and was suitable for analysis by Southern blotting. The high salt method can be used routinely for the extraction of DNA from sheep samples, increasing the number of samples processed and facilitating DNA extraction for genetic linkage analysis and diagnostic tests.
Stratégies de réécriture et gestion des contraintes d'écriture par des élèves de l'école élémentaire : ce que nous apprennent des écrits d'enfants sur l'écriture (2003)

International audience
Crystalline Boron Nanoribbons:  Synthesis and Characterization (2004)

Catalyst-free growth of boron nanoribbons was observed by pyrolysis of diborane at 630−750 °C and ∼200 mTorr in a quartz tube furnace. Nanodiffraction analysis indicates the nanoribbons are single crystal α-tetragonal boron. TEM images show the nanoribbon is covered by a 1−2 nm thick amorphous layer. Elemental analysis by EELS, EDX, and XPS shows the nanoribbons consist of boron with small amounts of oxygen and carbon. Infrared and Raman spectra are also reported.
Characterization of Activity and Expression of Isocitrate Lyase in <i>Mycobacterium avium</i> and <i>Mycobacterium tuberculosis</i> (1999)

Analysis by two-dimensional gel electrophoresis revealed that Mycobacterium avium expresses several proteins unique to an intracellular infection. One abundant protein with an apparent molecular mass of 50 kDa was isolated, and the N-terminal sequence was determined. It matches a sequence in the M. tuberculosis database (Sanger) with similarity to the enzyme isocitrate lyase of both Corynebacterium glutamicum and Rhodococcus fascians. Only marginal similarity was observed between this open reading frame (ORF) (termed icl) and a second distinct ORF (named aceA) which exhibits a low similarity to other isocitrate lyases. Both ORFs can be found as distinct genes in the various mycobacterial databases recently published. Isocitrate lyase is a key enzyme in the glyoxylate cycle and is essential as an anapleurotic enzyme for growth on acetate and certain fatty acids as carbon source. In this study we express and purify Icl, as well as AceA proteins, and show that both exhibit isocitrate lyase activity. Various known inhibitors for isocitrate lyase were effective. Furthermore, we present evidence that in both M. avium and M. tuberculosis the production and activity of the isocitrate lyase is enhanced under minimal growth conditions when supplemented with acetate or palmitate.
Dimensionally aware genetic programming (1999)

Spica casting compared to Bryant's traction is associated with shorter hospitalization following complete primary repair of exstrophy and does not have a significant difference in the rate of complications. In our longitudinal cohort study with long-term followup spica cast was safe and effective for patients with bladder exstrophy, and should be considered an acceptable method of immobilization.
Studies on Teaching Strategies for Italian Grade Test: Taking the Writing Part as an Example (2017)

This article has first introduced that Italian preparatory course training should be with language test as the teaching baton based on current overseas study data and policies. Targeting at the writing part in grade test, the author has collected and organized for 90 students’ writing paper, and analyzed students’ mistakes, pointed out the mistake reasons, and meanwhile revealed the influence of language negative transfer on students. The author has suggested that “metacognitive strategy” should be introduced into writing teaching, and proposed relevant teaching strategies.
A green FFT processor with 2.5-GS/s for IEEE 802.15.3c (WPANs) (2010)

This paper presents a high-throughput FFT processor for IEEE 802.15.3c (WPANs) standard. To meet the 2.59 Gigasample/s throughput requirements, radix-16 FFT algorithm is adopted and reformulated to an efficient form so that the required number of butterfly stages is reduced and the proposed radix-16 FFT butterfly processing element (PE) can be optimally pipelined. Specifically, the radix-16 butterfly PE consists of one radix-4 and two radix-2 cascaded pipelined butterfly units. It facilitates low-complexity realization of radix-16 butterfly operation and high operation speed due to the optimized pipelined structure. Hardware reuse and low-power schemes are also devised to reduce both area and power consumption. Moreover, a multibank memory scheme is used to support up to 16-times I/O capability of a single-bank memory, which can greatly reduce the input/output time of FFT data samples. As a result, the proposed radix-16 FFT processor is area-efficient with high data processing rate and hardware utilization efficiency. The EDA synthesis results show that the proposed design has 2.59 GS/s throughput and it consumes 103.5 mW by using UMC 90nm process.
Compressed sampling reconstruction of hyperspectral images based on spectral prediction (2015)

With increasing amounts of hyperspectral images (HSI) and the limitations of the memory requirements, compressive techniques for hyperspectral images have attracted extensive research efforts in recent years. The main difficulty of applying compressed sampling (CS) theory to compression and reconstruction of hyperspectral images lies in using the spatial correlation and spectral correlation of hyperspectral images. In this paper, a reconstruction algorithm of hyperspectral images taking advantage of two‐dimensional compressed sampling (2DCS) and two‐dimensional total variation (2DTV) incorporating spectral prediction (SP) is investigated. In the sampling process, the hyperspectral images are divided into reference bands and common bands, and all bands are sampled using 2DCS independently. In the reconstruction process, the reference bands are reconstructed by 2DTV first. In order to improve the reconstruction quality of common bands, spectral prediction utilizing the spectral correlation between reference bands and common bands is conducted. Then the spectral compensation is computed by using a combination of the prediction value and the initial approximation for the common bands. The residual between the compensation value and the original value is obtained to revise the approximation for the common bands. The algorithm is implemented in an iterative manner to enhance the performance. Experimental results on popular hyperspectral datasets reveal that the proposed algorithm exploiting spectral prediction outperforms the algorithm 2DCS‐2DTV, which does not use spectral correlation, as well as the state‐of‐the‐art algorithms in terms of peak signal‐to‐noise ratio (PSNR). In particular, when the sampling rate of the reference bands is higher than that of the common bands, the proposed algorithm would improve the reconstruction quality dramatically. © 2015 Institute of Electrical Engineers of Japan. Published by John Wiley & Sons, Inc.
The Black Lion (1976)

The Black LionThere was a red flag fluttering from the house at the corner, there were a few holes in it and it was a bit short. One glanceat the bullet marks on the walls was enough to tell the Black Lion that they had been here.And the minute his glance sUpped down from the shattered plaster on the walls to the two men of the "R" guard, banging the door behind them, he knew for certain that they were still there.He hitched his trousers up."FoUow me!" he said briefly.With his tousled head he gestured to the two at his side to foUow him."What's going on there?" asked one of them.But they sUpped in after him.
Logan going to the dogs under Newman (2014)

Queensland racing Minister Steve Dickson has just given the green light to a new greyhound racing track at Cronulla Park in Logan, one of Australia's fastest-growing urban corridors.
Monolithic capillary gel electrophoresis stage with on-chip detector (2002)

This paper describes the fabrication and initial measurements of an electrophoresis stage integrated with a substrate detector. The device is fabricated on a single silicon substrate using a 10-mask process. The separation channels are constructed with a polysilicon layer deposited on the surface of a substrate containing a buried, highly sensitive diffusion radiation detector. The buried detector can resolve single beta decay events from /sup 33/P- and /sup 32/P-labeled DNA. Initial experiments show migration of radioactive species in the channel at gel fields of 27 V cm/sup -1/.