gebhart/patrepeval huggingface dataset:
Gain-of-function genetic screening identifies the antiviral function of TMEM120A via STING activation (2022)

Zika virus (ZIKV) infection can be associated with neurological pathologies, such as microcephaly in newborns and Guillain-Barre syndrome in adults. Effective therapeutics are currently not available. As such, a comprehensive understanding of virus-host interactions may guide the development of medications for ZIKV. Here we report a human genome-wide overexpression screen to identify host factors that regulate ZIKV infection and find TMEM120A as a ZIKV restriction factor. TMEM120A overexpression significantly inhibits ZIKV replication, while TMEM120A knockdown increases ZIKV infection in cell lines. Moreover, Tmem120a knockout in mice facilitates ZIKV infection in primary mouse embryonic fibroblasts (MEF) cells. Mechanistically, the antiviral activity of TMEM120A is dependent on STING, as TMEM120A interacts with STING, promotes the translocation of STING from the endoplasmic reticulum (ER) to ER-Golgi intermediate compartment (ERGIC) and enhances the phosphorylation of downstream TBK1 and IRF3, resulting in the expression of multiple antiviral cytokines and interferon-stimulated genes. In summary, our gain-of-function screening identifies TMEM120A as a key activator of the antiviral signaling of STING.
Self-regulating dynamic control of electroosmotic flow in capillary electrophoresis (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSelf-regulating dynamic control of electroosmotic flow in capillary electrophoresisHuan Tsung. Chang and Edward S. YeungCite this: Anal. Chem. 1993, 65, 5, 650–652Publication Date (Print):March 1, 1993Publication History Published online1 May 2002Published inissue 1 March 1993https://pubs.acs.org/doi/10.1021/ac00053a028https://doi.org/10.1021/ac00053a028research-articleACS PublicationsRequest reuse permissionsArticle Views103Altmetric-Citations18LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
The K121Q Polymorphism of the PC-1 Gene Is Associated With Insulin Resistance but not With Dyslipidemia (2003)

In healthy normoglycemic Finnish subjects, the K121Q polymorphism of the PC-1 gene is associated with insulin resistance but not with impaired insulin secretion or dyslipidemia.
PUBLICATION REVIEWS (2013)

Anne Wilson: Wind/Rewind/Weave, Anne Wilson (2011) Chicago: University of Chicago Press, 159 pp, ISBN: 978-0-945-32322-8, p/bk, $30.00/£19.50 Stephen De Staebler: Matter and Spirit, Timothy Anglin Burgard (2012) San Francisco: The Fine Arts Museum of San Francisco in association with University of California Press, 240 pp, ISBN: 978-0-520-27230-9, p/bk, $34.95
Enzymatic Pre-Treatment Increases the Protein Bioaccessibility and Extractability in Dulse (Palmaria palmata) (2016)

Several common protein extraction protocols have been applied on seaweeds, but extraction yields have been limited. The aims of this study were to further develop and optimize existing extraction protocols and to examine the effect of enzymatic pre-treatment on bioaccessibility and extractability of seaweed proteins. Enzymatic pre-treatment of seaweed samples resulted in a three-fold increase in amino acids available for extraction. Combining enzymatic pre-treatment with alkaline extraction resulted in a 1.6-fold increase in the protein extraction yield compared to a standard alkaline extraction protocol. A simulated in vitro gastrointestinal digestion model showed that enzymatic pre-treatment of seaweed increased the amount of amino acids available for intestinal absorption 3.2-fold. In conclusion, enzymatic pre-treatment of seaweeds is effective for increasing the amount of amino acids available for utilization and may thus be an effective means for increasing the utilization potential of seaweed proteins. However, both the enzymatic pre-treatment protocol and the protein extraction protocol need further optimization in order to obtain optimal cost-benefit and results from the in vitro gastrointestinal digestion model need to be confirmed in clinical models.
Strong security for distributed file systems (2002)

We have developed a scheme to secure network-attached storage systems against many types of attacks. Our system uses strong cryptography to hide data from unauthorized users; someone gaining complete access to a disk cannot obtain any useful data from the system, and backups can be done without allowing the super-user access to unencrypted data. While denial-of-service attacks cannot be prevented, our system detects forged data. The system was developed using a raw disk, and can be integrated into common file systems. We discuss the design and security tradeoffs such a distributed file system makes. Our design guards against both remote intruders and those who gain physical access to the disk, using just enough security to thwart both types of attacks. This security can be achieved with little penalty to performance. We discuss the security operations that are necessary for each type of operation, and show that there is no longer any reason not to include strong encryption and authentication in network file systems.
Automated sequencing of insoluble peptides using detergent. Bacteriophage fl coat protein. (1977)

Peptides which are highly nonpolar and insoluble under moderate conditions of pH and ionic strength cannot be subjected to automated sequence analysis. We report a method for solubilization of one such peptide, bacteriophage fl coat protein, by chemical modification in the presence of sodium dodecyl sulfate. Following this treatment the 50-residue peptide was degraded stepwise in an automated sequenator using a single cleavage Quadrol program with high repetitive yield through residue 47. We also report a modified program using detergent incorporated into dimethylallylamine buffer which permitted sequencing with high repetitive yields for at least the first 18 residues of the unmodified and otherwise highly insoluble coat protein. The presence of detergent caused no observable difficulties in detection of residues by gas chromatography, thin layer chromatography, or amino acid analysis.
Terahertz emission from black silicon (2008)

We report on a terahertz emitter made out of black silicon. The black surface structure absorbs the whole optical pump power in the very surface. In contrast to expectations for indirect semiconductors, the black structure shows an emission in the terahertz range. The emitted radiation of the black silicon crystal is characterized for different parameters using terahertz time-domain spectroscopy.
Invisible Gold Paragenesis and Geochemistry in Pyrite from Orogenic and Sediment-Hosted Gold Deposits (2020)

LA-ICPMS analysis of pyrite in ten gold deposits is used to determine the precise siting of invisible gold within pyrite, and thus the timing of gold introduction relative to the growth of pyrite and related orogenic events. A spectrum of invisible gold relationships in pyrite has been observed which suggests that, relative to orogenic pyrite growth, gold introduction in some deposits is early at the start of pyrite growth; in other deposits, it is late toward the end of pyrite growth and in a third case, it may be introduced at the intermediate stage of orogenic pyrite growth. In addition, we report a distinct chemical association of invisible gold in pyrite in the deposits studied. For example, in the Gold Quarry (Carlin type), Mt Olympus, Macraes and Konkera, the invisible gold is principally related to the arsenic content of pyrite. In contrast, in Kumtor and Geita Hill, the invisible gold is principally related to the tellurium content of pyrite. Other deposits (Golden Mile, Bendigo, Spanish Mountain, Witwatersrand Carbon Leader Reef (CLR)) exhibit both the Au-As and Au-Te association in pyrite. Some deposits of the Au-As association have late orogenic Au-As-rich rims on pyrite, which substantially increase the value of the ore. In contrast, deposits of the Au-Te association are not known to have Au-rich rims on pyrite but contain nano- to micro-inclusions of Au-Ag-(Pb-Bi) tellurides.
On hybrid systems and closed-loop MPC systems (2002)

The following five classes of hybrid systems were recently proven to be equivalent: linear complementarity, extended linear complementarity, mixed logical dynamical systems, piecewise affine systems and max-min-plus-scaling systems. Some of the equivalences were obtained under additional assumptions, such as boundedness of certain system variables. In this paper, for linear or hybrid plants in closed-loop with a model predictive control (MPC) controller based on a linear model fulfilling linear constraints on input and state variables and utilizing a quadratic cost criterion, we provide a simple and direct proof that the closed-loop system is a subclass of any of the former five classes of hybrid systems. This result is of extreme importance, as it opens up the use of tools developed for the mentioned hybrid model classes, such as (robust) stability and safety analysis tools, to study closed-loop properties of MPC.
Macroeconomic reform and growth in Africa : adjustment in Africa revisited (1994)

The 1994 World Bank study, "Adjustment in Africa: reforms, results, and the road ahead, "assessed the extent of, and economic payoffs from, policy reform in 29 countries in sub-Saharan Africa in the mid-1980s and 1990s. Here, the authors update the results of that report with 1992 macroeconomic data and explore some issues in more detail. The conclusions of the earlier report still hold: improved policies are still associated with improved performance, but countries fall short of having sound policies. In fact, the 1991-92 policy stance was not as strong as the 1990-91 stance, reflecting the slow, fragile, and often reversal-prone nature of macroeconomic reform in Africa. Getting the real exchange rate right and reducing the fiscal deficit should be the top priority for restoring growth. Countries that significantly reduced their budget deficits and reduced the black market premium (by devaluing) enjoyed the greatest payoffs from reform. Devaluation of the CFA franc in January 1994 represents a real opportunity for the CFA franc zone countries to restore growth. Many countries have made considerable progress in moving toward competitive real exchange rates. There still remains the challenge of reducing budget deficits in ways consistent with poverty-reducing growth. Hence, the need to reorient public spending to the essential tasks of government, especially providing social services. Reform in two areas will be important to sustaining fiscal reform: implicit subsidies to public enterprises must be cut, and the cost of restructuring the banking sector must not be absorbed by the budget. Policy reforms undertaken so far have paid off in higher growth rates, but the level of growth is still too low to sustain rapid rates of poverty reduction. Increased growth seems to have come more from efficient use of existing capacity than from new investments. Only steady and increased policy reform will convince investors of the credibility of reform and thus of a more favorable investment climate.
Novel Application of the Flotation Technique To Measure the Wettability Changes by Ionically Modified Water for Improved Oil Recovery in Carbonates (2016)

The accurate determination of the wetting condition of carbonate reservoirs is a prerequisite for the selection of any enhanced oil recovery (EOR) fluid. Most carbonate formations are initially oil-wet to intermediate-wet at reservoir conditions. In order to correctly chose an EOR fluid to alter the wettability, we need to understand the initial wetting conditions and design an ionically modified water (advanced water) to alter wettability and improve oil recovery. If a reservoir has already been reached to the optimum wetting conditions by injecting formation water or any other fluid, then there is no need for ionically modified water. A number of methods have been devised to identify the wetting conditions including contact angle measurements, spontaneous imbibition, and chromatographic separation, etc. But contact angle measurement requires surfaces that lack natural surface roughness, spontaneous imbibition tests take months, and chromatographic separation is feasible only for core flooding in sulfate free carbonates at low temperature. A novel application of the well-established technique known as flotation was used in this study to measure the oil-wet and water-wet percent of pure biogenic chalk (Dan Chalk from Denmark). It is an accurate, fast, and most reliable method to quantitatively measure the water-wet and oil-wet fractions of a reservoir rock. It determines the potential of advanced water to improve wettability within days, instead of measurements that can take months and require expensive equipment. Using this technique we were able to quantify the wettability alteration caused by low salinity and potential determining anions (PDAs) such as SO42–, BO33–, and PO43–. The wettability data show maximum oil recovery by dilution is coincident with maximum wettability alteration. The experiments also show that the presence of sulfate or borate enhances wettability alteration by dilution. Moreover, the combined and individual effect of potential scale forming ions (Ba2+ and Sr2+) on wettability restoration was identified. The effect of interfacial tension (IFT) on the measured wettability alteration and the amount of oil attached to the water-wet percent of rock was also determined.
The damping of capillary–gravity waves at a rigid boundary (1987)

The frequency and damping rate of surface capillary-gravity waves in a bounded region depend on the conditions imposed where the free surface makes contact with the boundary. Extreme cases are when the free surface meets the boundary orthogonally, as in the case of pure gravity waves, and when the contact line remains fixed throughout the motion. An edge condition that models to some extent the dynamics associated with moving contact lines, but not contact-angle hysteresis, is given by making the slope of the free surface at contact proportional to its velocity. This model, which includes the two extreme cases, is used to obtain the frequency and damping rate of a standing wave between two parallel vertical walls. The effect of viscosity in the boundary layers on the walls is included and it is shown that the dissipation associated with the surface forces can exceed that produced by viscosity. The results are compared with those obtained from a number of experimental investigations, in which damping rates too large to be attributed to viscous action have been measured.
Overexpression of the transcription factor RAP2.6 leads to enhanced callose deposition in syncytia and enhanced resistance against the beet cyst nematode Heterodera schachtiiin Arabidopsis roots (2013)

Our results showed that H. schachtii infection is accompanied by a downregulation of RAP2.6. It seems likely that the nematodes use effectors to actively downregulate the expression of this and other defense-related genes to avoid resistance responses of the host plant. Enhanced resistance of RAP2.6 overexpression lines seemed to be due to enhanced callose deposition at syncytia which might interfere with nutrient import into syncytia.
The SPC technique and low complexity turbo codes (2003)

This paper proposes a family of modified turbo codes, referred to as single parity check (SPC) turbo codes. The decoding cost, in terms of both operation number and storage usage, of the proposed scheme is significantly lower (5-10 times) than the standard turbo codes with similar performance. The scheme can also be used to alleviate the error floor problem and is very flexible for median to high rate code designs.
Channel Equalization Using Neural Networks: A Review (2010)

Equalization refers to any signal processing technique used at the receiver to combat intersymbol interference in dispersive channels. This paper reviews the applications of artificial neural networks (ANNs) in modeling nonlinear phenomenon of channel equalization. The literature associated with different feedforward neural network (NN) based equalizers like multilayer perceptron, functional-link ANN, radial basis function, and its variants are reviewed. Feedback-based NN architectures like recurrent NN equalizers are described. Training algorithms are compared in terms of convergence time and computational complexity for nonlinear channel models. Finally, some limitation of current research activities and further research direction is provided.
Toolpath design for additive manufacturing using deep reinforcement learning (2020)

Toolpath optimization of metal-based additive manufacturing processes is currently hampered by the high-dimensionality of its design space. In this work, a reinforcement learning platform is proposed that dynamically learns toolpath strategies to build an arbitrary part. To this end, three prominent model-free reinforcement learning formulations are investigated to design additive manufacturing toolpaths and demonstrated for two cases of dense and sparse reward structures. The results indicate that this learning-based toolpath design approach achieves high scores, especially when a dense reward structure is present.
Dopamine innervation of a subclass of local circuit neurons in monkey prefrontal cortex: ultrastructural analysis of tyrosine hydroxylase and parvalbumin immunoreactive structures (1998)

The ability of dopamine to regulate the cognitive functions of the prefrontal cortex (PFC) involves complex modulatory actions on GABA-containing local circuit neurons in addition to pyramidal cells. However, the subclasses of cortical neurons that receive direct dopamine input are not known. We sought to determine whether dopamine terminals innervate the subclasses of local circuit neurons that contain the calcium-binding protein parvalbumin (PV), namely the wide arbor and chandelier neurons that target pyramidal cell soma and axon initial segments respectively. Sections through area 9 of five monkeys were labeled with immunoperoxidase for tyrosine hydroxylase (TH), to identify dopamine terminals, and with immunogold-silver for PV. Electron microscopic examination of the middle cortical layers (IIIb-IV) revealed that TH-positive terminals were sometimes directly apposed to PV-labeled dendrites, and approximately one-third of these contacts exhibited morphological features that are typically associated with symmetric synapses. In contrast, TH-immunolabeled terminals in the superficial layers (I-IIIa) were less frequently apposed to PV-positive dendrites, and none of these contacts exhibited synapse-like morphology. These findings, in concert with previous studies of GABA- or calretinin-containing local circuit neurons, suggest that dopamine's modulatory action in the PFC involves selective effects on only certain interneuron populations, including those that mediate potent inhibitory actions on pyramidal cells.
A design of wideband 3-dB coupler with N-section microstrip tandem structure (2005)

New design equations and a 3-dB microstrip coupler example for N-section tandem connected structure with wide bandwidth are presented. The proposed four-port S-parameters and equations are obtained from a port reduction method. The designed microstrip 3-dB coupler not only does not need high impedance lines, but also uses tight coupling gaps differently from conventional couplers such as Lange couplers, parallel coupled line couplers, etc. The measured data agrees well with the expected data, which show a wide bandwidth of 42%, an amplitude imbalance of /spl plusmn/0.5 dB, a phase unbalance of 1.0/spl deg/, and isolation characteristics of 15 dB at the band of 3.6 to 5.5 GHz.
Methods of theophylline assay and therapeutic monitoring of this drug. (1990)

The purpose of this article is to review various analytical methods of monitoring plasma theophylline. This article was investigated by the "Drug Commission" of SFBC (Société Française de Biologie Clinique). The primary objective is to provide the "know-how", particular for this analysis, which allows the choice between various analytical methods available: immunochemical or physiochemical ones. The techniques described are not necessarily the best, they are approved and tested methods which are the most frequently used in routine practice. The proposed immunochemical methods are: absorption spectroscopy methods: Enzyme ImmunoAssay (EIA), Enzyme Multiplied ImmunoAssay Technique (EMIT); Reflectance spectroscopy method: Apoenzyme Reactivation Immunoassay System (ARIS); Fluorometry spectroscopy method: Substrate Labeled FluoroImmunoAssay (SLFIA); Fluorometry spectroscopy on solid base; Polarization fluorescence spectroscopy ImmunoAssay (FPIA); Turbidimetric measurements: Particle Enhanced Turbidimetric Inhibition ImmunoAssay (PETINIA); Nephelometric measurement: Nephelometric Inhibition ImmunoAssay (NIIA). And the proposed physicochemical methods are: High Performance Liquid Chromatography (HPLC), Gas Chromatography (GC). The second objective is a review of pharmacological properties of theophylline, necessary for a good understanding of therapeutic drug monitoring: intestinal resorption, distribution, metabolism and elimination, drug interactions, dose/response relationship, physiopathological variations and proposed "predictive" "theophylline test". The authors conclude that because of the multiplicity of methodologies used in theophylline therapeutic monitoring the choice of one of them is not easy. The best way to compare different techniques available would be the use of a "reference material" for theophylline monitoring and a quality control network between different clinical pharmacological laboratories.
An Infrastructure to Support Secure Internet Routing (2012)

This document describes an architecture for an infrastructure to
support secure Internet routing. The foundation of this architecture
is a public key infrastructure (PKI) that represents the allocation
hierarchy of IP address space and Autonomous System Numbers;
certificates from this PKI are used to verify signed objects that
authorize autonomous systems to originate routes for specified IP
address prefixes. The data objects that comprise the PKI, as well as
other signed objects necessary for secure routing, are stored and
disseminated through a distributed repository system. This document
also describes at a high level how this architecture can be used to
add security features to common operations such as IP address space
allocation and route filter construction.
Robot-Assisted Targeted Gait Training (2023)

Compliant environments, like those created on the VST, may be a beneficial tool as they can: reduce high-impact forces during running and walking, significantly engage the muscles surrounding the hip and knee allowing for targeted training and rehabilitation, and assist in identifying and training high fall-risk individuals.