gebhart/patrepeval huggingface dataset:
Granulocytic and monocytic differentiation of CD34hi cells is associated with distinct changes in the expression of the PU.1- regulated molecules, CD64 and macrophage colony-stimulating factor receptor (1996)

The present study investigated the possibility that macrophage colony-stimulating factor (M-CSF) responsiveness of hematopoietic progenitor cells is regulated at the level of receptor expression and that M-CSF receptor (M-CSFR) may be used as an early marker of monocyte lineage commitment. Immunofluorescence measurements with an anti-M-CSFR antibody showed that 44% +/- 5% of CD34hi cells expressed the receptor. The M-CSFR was present on progenitor cells that were positive for the granulo-monocytic marker CD64, but not on primitive, erythroid, or lymphoid progenitors. The CD34hiCD64+ population could be divided into subsets of M-CSFRhi and M-CSFRlo cells. In addition, a subset of CD34hiCD64-M-CSFRhi cells was found. CD34+ cells that were positive for M-CSFR, CD64, or both gave rise exclusively to granulo-monocytic cells, and 65% of the granulomonocytic colony-forming cells in the CD34+ population were recovered from these cells. Approximately 70% of the colony-forming cells (CFCs) derived from CD34hiM-CSFRhi cells were macrophage colony-forming units (CFU-M), whereas 91% of the CFCs in the CD34hiCD64+M-CSFRlo population were granulocyte colony-forming units (CFU-G). The M-CSFRhi cells with the highest frequency of colony-forming and bipotent cells and largest average colony size were found in the CD64- subset, indicating that M-CSFR appears earlier than CD64 during monocyte development. After 60 hours in culture, a subset of the CD34hiM-CSFRhi cells had downmodulated M-CSFR (29% to 38%). This population gave rise almost exclusively to granulocytes, whereas the cells that remained M-CSFRhi gave rise exclusively to monocytes. In all experiments, the M-CSFRhi population responded to M-CSF, whereas minimal responses were observed among M-CSFRlo cells. These results suggest that M-CSF target specificity among human hematopoietic progenitor cells is determined by lineage-specific regulation of the M-CSFR and show that M-CSFR is a useful marker to discriminate between monocytic and granulocytic progenitor cells.
Unsupervised Charting of Wireless Channels (2018)

Future wireless communication systems will rely on large antenna arrays at the infrastructure base stations (BSs) to serve multiple users with high data rates in a single cell. We demonstrate that the availability of high-dimensional channel state information (CSI) acquired at such multi-antenna BSs enables one to learn a chart of the radio geometry, which captures the spatial geometry of the users so that points close in space are close in the channel chart, using no other information than wireless channels of users. Specifically, we propose a novel unsupervised framework that first extracts channel features from CSI which characterize large-scale fading effects of the channel, and then uses specialized dimensionality reduction tools to construct the channel chart. The channel chart can, for example, be used to perform (relative) user localization, predict cell hand-overs, or guide scheduling tasks, without accessing location information from global navigation satellite systems.
Usefulness of Immunohistochemical Diagnosis of Streptococcus pneumoniae in Formalin-Fixed, Paraffin-Embedded Specimens Compared With Culture and Gram Stain Techniques (2007)

Streptococcus pneumoniae is the most frequent cause of pneumonia and meningitis. Because S pneumoniae can colonize the upper respiratory tract and antibiotic treatment may inhibit growth, culture-based diagnosis can be problematic. An immunohistochemical assay using a polyclonal antibody against pneumococci was used to test formalin-fixed, paraffin-embedded tissue samples from 46 patients for whom bacterial culture results were available. Samples from 26 patients demonstrated pneumococcal antigens in areas of pneumonia, meningitis, or osteomyelitis or within circulating inflammatory cells. Various specimens from 18 patients grew S pneumoniae, whereas 8 had cultures that grew mixed bacteria or had no growth but were polymerase chain reaction–positive for the S pneumoniae ltyA gene. Pneumococcal antigens were not present in 20 cases (7 grew Streptococcus pyogenes; 9, Staphylococcus aureus; and 4, Haemophilus influenzae). Compared with culture, the immunohistochemical assay showed 100% sensitivity and 71% specificity. Immunohistochemical analysis has the diagnostic advantage of correlating host inflammatory reaction with presence of pneumococci.
Changes in angiotensin II type 1 receptor signalling pathways evoked by a monoclonal antibody raised to the N-terminus (2008)

The extracellular N-terminus of G-protein-coupled receptors may be involved in signalling events. We examined this in the angiotensin II type 1 receptor (AT1-R) using monoclonal antibody 6313/G2, raised against a conserved sequence in the N-terminal domain, and found it evokes inhibitory and stimulatory responses. In rat aortic smooth muscle cell (RASMC) primary cultures, 6313/G2 (2.5 microg/ml) inhibited both basal and angiotensin II (Ang II; 10(-7) mol/l)-stimulated [H(3)]thymidine incorporation. Exposure to 6313/G2 gave sustained increases in phosphorylated protein kinase Calpha (PKCalpha) but gave a decrease in phosphorylated p44/42 extracellular signal-regulated kinases (ERK1/2) sustained from 10 min to 48 h compared with untreated control RASMC. In contrast, Ang II had no effect on PKCalpha, and, though it is acutely stimulatory (up to 5 min), it had no sustained effect on ERK1/2 either. Using Fura-2 and microfluorimetry, 6313/G2 added alone induced a transient increase in intracellular calcium ([Ca2+](i)), with a characteristic response curve different from that of Ang II itself. The antibody was without effect on an Ang II-stimulated activator protein-1 reporter system, though it reduced unstimulated reporter activity. Such discriminatory effects on intracellular signalling suggest that the AT1-R N-terminus itself might be a target for therapeutic intervention in chronic vascular disease.
Improvement to the adjustable collimator for neutron therapy (1985)

In 1981 the authors reported on an adjustable collimator for neutron therapy using 16 MeV deuterons on a Be-Cu target (Bewley et al., ibid., vol.26, p.711-14, 1981). They pointed out that the collimation was sharper in the vertical direction than in the horizontal and suggested that the performance could be improved by adding a trimmer in the horizontal direction. This has now been done and the results are presented. In 1981 it was suggested that the thickness of the trimmer should be taken of the main horizontal jaws, keeping the total length the same. It was not practicable to modify the existing collimator in this way, so instead 10 cm was taken off the vertical trimmer and the horizontal trimmer was fitted into the space. It is shown that the sharpness of the profile has been improved considerably in the horizontal plane with only a little loss vertically. The horizontal direction is the more important for radiotherapy of tumours of the head and neck when the patient is treated in the sitting position, as is nearly always the case. Further from the beam the trimmer has made little difference except for a rearrangement between the vertical and horizontal directions.
Ramp load/unload friction dependence on temperature, velocity and ramp material (2001)

A simple tool was developed to evaluate ramp/tab friction during dynamic ramp load/unloads on component level test stands for hard-disk drives. By extracting key attributes of each load/unload time-varying friction cycle, multiple valuable engineering studies were performed. A screening test of candidate ramp materials revealed a strong correlation between friction and wear, with acetal homopolymer and aliphatic polyketone being the most attractive choices. The rate of change of friction with temperature can significantly vary between materials. Data are also presented on friction versus load/unload cycles (showing the onset of wear), load velocity and tab roughness. With knowledge of extreme friction values on each section of the load ramp (slope, top, detent), their influence on actuator torque during load and unload can be calculated and included in tolerance analyses. It is shown that friction can play a significant role in multi-disk drives.
Photomechanical bending mechanics of polydomain azobenzene liquid crystal polymer network films (2012)

Glassy, polydomain azobenzene liquid crystal polymer networks (azo-LCNs) have been synthesized, characterized, and modeled to understand composition dependence on large amplitude, bidirectional bending, and twisting deformation upon irradiation with linearly polarized blue-green (440–514 nm) light. These materials exhibit interesting properties for adaptive structure applications in which the shape of the photoresponsive material can be rapidly reconfigured with light. The basis for the photomechanical output observed in these materials is absorption of actinic light by azobenzene, which upon photoisomerization dictates an internal stress within the local polymer network. The photoinduced evolution of the underlying liquid crystal microstructure is manifested as macroscopic deformation of the glassy polymer film. Accordingly, this work examines the polarization-controlled bidirectional bending of highly concentrated azo-LCN materials and correlates the macroscopic output (observed as bending) to measured blocked stresses upon irradiation with blue-green light of varying polarization. The resulting photomechanical output is highly dependent on the concentration of crosslinked azobenzene mesogens employed in the formulation. Experiments that quantify photomechanical bending and photogenerated stress are compared to a large deformation photomechanical shell model to quantify the effect of polarized light interactions with the material during static and dynamic polarized light induced deformation. The model comparisons illustrate differences in internal photostrain and deformation rates as a function of composition and external mechanical constraints.
The Direct Measurement of Damping Coefficients Using an Inverse Eigenvalue Method (1992)

A direct measurement technique to identify the viscous damping coefficient of one-dimensional devices has been developed. The frequency- dependent properties of the damping coefficient are inherent in this formulation. It has been shown that the variance in the prediction of the damping coefficient is small. The experimental apparatus is extremely easy to build, and the raw data necessary to make the calculations are readily obtained with standard modal techniques.
The Use of Antibodies to Coagulation Factors for Anticoagulant Therapy (2004)

Current treatments for preventing thrombotic diseases are associated with a significant risk of bleeding. Improved anticoagulant agents are therefore still required. The specificity and pharmacokinetics properties of monoclonal antibodies to coagulation factors allow novel anticoagulation approaches. Treatment with human antibodies or humanized mouse monoclonal antibodies should avoid unacceptable side effects due to immune response to the drug. Such antibodies were developed against three coagulation factor: Tissue factor (TF), Factor IX (FIX) and Factor VIII (FVIII). A fully humanized antibody was successfully derived from a mouse monoclonal antibodies to TF. In vivo studies with monoclonal antibodies to TF demonstrated efficient antithrombotic activity. Anti-TF antibodies may also prove useful in cardiovascular disorders and cancer, given the role of TF in these diseases. Mouse and human monoclonal antibodies to FIX were also efficient to prevent thrombosis in animal models of venous and arterial thrombosis and in stroke. A humanized anti-FIX antibody was tested in phase I study in healthy volunteers. The pharmacokinetics of the antibody were determined by the rapid formation of stable complexes with newly synthesised FIX. Human anti-FVIII antibodies inhibiting only partially FVIII activity were recently described. Investigations in mice have established that treatment with such anti-FVIII antibodies is efficient to prevent deep vein thrombosis. Given the low concentration of FVIII in plasma and the long half-life of antibody, treatment with anti-FVIII antibody could be very convenient, allowing one administration every month. Altogether, monoclonal antibodies to coagulation factor appear as promising novel antithrombotic drugs.
Spread-time code-division multiple access (1995)

An alternative code-division multiple-access (CDMA) scheme to spread spectrum (SS), called spread time (ST) is proposed for bandlimited multiple-access channels. ST-CDMA can be considered the time-frequency dual of SS-CDMA. In ST-CDMA pseudorandom (PN) sequences are assigned to each user, and the Fourier transform of the transmitted pulse for a given user is determined by modulating the phase of the desired transmitted spectrum by the user's PN-sequence. The transmitted data for a particular user can be recovered by sampling the output of a filter matched to the user's pulse. Implementations are described in which surface acoustic wave devices are used to perform the matched filtering or Fourier transformation. Averaged signal-to-interference plus noise ratio (SIR) and spectral efficiency are computed for both asynchronous ST and direct-sequence SS-CDMA systems, assuming an arbitrary channel transfer function H(f), which is the same between all pairs of users. The results are the same for SS and ST provided that the magnitude of the Fourier transform of the chip shape in the SS system is the same as the magnitude of the Fourier transform of the ST pulse shape. The main advantage of the ST technique Is the flexibility with which the transmitted spectrum can be selected. We derive the transmitted spectrum that maximizes the SIR subject to an average power constraint.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Isolation and Expansion of Adult Cardiac Stem/Progenitor Cells in the Form of Cardiospheres from Human Cardiac Biopsies and Murine Hearts (2012)

The successful isolation and ex vivo expansion of resident cardiac stem/progenitor cells from human heart biopsies has allowed us to study their biological characteristics and their applications in therapeutic approaches for the repair of ischemic/infarcted heart, the preparation of tissue-engineered cardiac grafts and, possibly, the design of cellular kits for drug screening applications. From the first publication of the original method in 2004, several adjustments and slight changes have been introduced to optimize and adjust the procedure to the evolving experimental and translational needs. Moreover, due to the wide applicability of such a method (which is based on the exploitation of intrinsic functional properties of cells with regenerative properties that are present in most tissues), the key steps of this procedure have been used to derive several kinds of tissue-specific adult stem cells for preclinical or clinical purposes.In order to define the original procedure, complete with the up-to-date modifications introduced through the years, an exhaustive description of the current protocol is performed in this chapter, with particular attention in highlighting critical steps and troubleshoots. The procedure described here consists of modular steps, that could be employed to derive cells from any kind of tissue biopsy, and needs to be considered the gold standard of all the so-called "explant methods" or "cardiosphere methods," and it represents a milestone in the clinical translation of autologous cell therapy.
Helium gas jet spectral calibration of acoustic emission transducers and systems (1976)

The excitation and spectral calibration of acoustic emission systems using a helium gas jet is shown to produce system independent acoustic emission spectral information. The calibration source makes no mechanical contact with the specimen or structure under investigation and can be conveniently used for specimen or structural response mapping.
Ultra-Wideband Step Notch Array using stripline feed (2007)

The novel stripline feed design operates as claimed. This new feed design will allow integration of vertical 3-D TEM wideband notch array to a PCB. The exploitation of the ultra-wideband high scan angle ESA built using a tile-based architecture is now possible.
Plasmonic-electrochemical dual modality microfluidic sensor for cancer biomarker detection (2017)

A dual-modality microfluidic immunosensor is reported for the detection of cancer biomarker proteins using graphene oxide assembled periodic gold nanoposts array. The sensor uniquely provides both electrochemical and surface plasmon resonance (SPR) signatures on a single platform. Sensitivities for both electrochemical and SPR detection schemes are found to be 3.94 μA/μM/cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> in a range of 1×10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-15</sup> M to 0.1×10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-6</sup> M, and 2.9 nm/μM/cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> in a range of 1×10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-15</sup> M to 1×10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-9</sup> M, respectively, for the detection of epidermal growth factor receptor 2 (ErbB2) breast biomarker. The present dual-modality sensor approach enables improved detection reliability and precision, and reduced false reads.
Novel scheme for labeling XML trees based on bits-masking and logical matching (2013)

The eXtensible Mark-up Language rapidly has become a very powerful standard for the data exchange. Labeling schemes have been introduced to optimize data retrieval and query processing on XML database documents. This is done by providing labels that hold information about XML tree nodes. In this paper we introduce a novel labeling scheme XDAS whose labeling technique is inspired by IP addressing and subnetting technique used in computer networks. This technique is used when dividing a network into several sub-networks. Each sub-network is assigned a subnet mask that helps in identifying the parent network. So, this labeling scheme treats XML documents as a network with sub-networks and assigns labels for XML tree nodes using the masking technique. Experimental results show that XDAS, when compared to Dewey and Range labeling schemes, provides an efficient label size, disk space required to store labels and matching time required to identify relationships between nodes.
Synthetic Strategies for Tailoring the Physicochemical and Magnetic Properties of Hydrophobic Magnetic Ionic Liquids (2015)

Magnetic ionic liquids (MILs) are a subclass of ionic liquids (ILs) containing paramagnetic components and are readily manipulated by an external magnetic field. Due to their hydrophilic nature, very few applications of MILs in aqueous systems have been reported. In this study, three general classes of hydrophobic MILs including monocationic, symmetrical/unsymmetrical dicationic, and symmetrical/unsymmetrical tricationic MILs were synthesized and characterized. By tuning the structure of the MIL, various physicochemical properties including water solubility, magnetic susceptibility, and melting point were regulated. MILs synthesized with the benzimidazolium cation were shown to exhibit lower water solubility (0.1% (w/v)) when compared to those containing imidazolium cations (0.25% (w/v)). By incorporating asymmetry into the cationic component of the MIL, the melting point of dicationic MILs was lowered while the effective magnetic moment (μeff) and hydrophobicity remained unchanged. Tricationic MILs paired with three [FeCl3Br–] anions exhibited an μeff as high as 11.76 Bohr magnetons (μB), the highest ever reported for MILs. The synthetic strategies employed in this study facilitate the generation of hydrophobic MILs that show great promise for liquid–liquid extraction and catalytic studies where the MIL can be easily removed or in microfluidic applications where the MIL microdroplet can be manipulated by an external field.
Design of Polarization De-Multiplexer and PMD Compensator for 112 Gb/s Direct-Detect PDM RZ-DQPSK Systems (2010)

The design of a polarization de-multiplexer and a polarization mode dispersion compensator (PMDC) for direct-detect polarization division multiplexed (PDM) return-to-zero differential quadrature phase shift keying (RZ-DQPSK) systems are studied in detail. The impact of polarization dependent loss is studied in polarization de-multiplexers with different error detection configurations for both bit-aligned and bit-interleaved PDM systems. The level of the clock frequency of the combined pulse train of the two polarizations is proposed as the error signal for the PMDC. It enables the PMDC to work in the cancellation mode. Two separate control loops are proposed for the polarization de-multiplexer and the PMDC to allow them to work independently. The DGD tolerances for the one-stage and two-stage PMDC are measured and discussed. Finally the glitch problem in the polarization tuning algorithm is studied. An advanced dithering algorithm and the corresponding architecture of the polarization controller are proposed to solve the glitch problem.
Four-dimensional ultrafast electron microscopy of phase transitions (2006)

Reported here is direct imaging (and diffraction) by using 4D ultrafast electron microscopy (UEM) with combined spatial and temporal resolutions. In the first phase of UEM, it was possible to obtain snapshot images by using timed, single-electron packets; each packet is free of space-charge effects. Here, we demonstrate the ability to obtain sequences of snapshots ("movies") with atomic-scale spatial resolution and ultrashort temporal resolution. Specifically, it is shown that ultrafast metal-insulator phase transitions can be studied with these achieved spatial and temporal resolutions. The diffraction (atomic scale) and images (nanometer scale) we obtained manifest the structural phase transition with its characteristic hysteresis, and the time scale involved (100 fs) is now studied by directly monitoring coordinates of the atoms themselves.
Materials processing using a variable frequency microwave furnace (1993)

We describe a materials processing system that uses a high power traveling wave tube (TWT) as the microwave source. The TWT provides approximately one octave bandwidth and variable power levels up to 2 kW into a multimode cavity. By controlling the frequency, efficient coupling to the load can be maintained even as the load`s dielectric properties change. Alternatively, can be used as a means of mode stirring at rates far beyond those attainable through mechanical stirring. The system has been tested for sintering alumina ceramics, annealing a tungsten penetrator alloy, curing epoxy resin, and depositing diamond films from a microwave plasma.