gebhart/patrepeval huggingface dataset:
Liposome-mediated transfection of mature taste cells (2005)

The introduction and expression of exogenous DNA in neurons is valuable for analyzing a range of cellular and molecular processes in the periphery, e.g., the roles of transduction-related proteins, the impact of growth factors on development and differentiation, and the function of promoters specific to cell type. However, sensory receptor cells, particularly chemosensory cells, have been difficult to transfect. We have successfully introduced plasmids expressing green and Discosoma Red fluorescent proteins (GFP and DsRed) into rat taste buds in primary culture. Transfection efficiency increased when delaminated taste epithelium was redigested with fresh protease, suggesting that a protective barrier of extracellular matrix surrounding taste cells may normally be present. Because taste buds are heterogeneous aggregates of cells, we used alpha-gustducin, neuronal cell adhesion molecule (NCAM), and neuronal ubiquitin carboxyl terminal hydrolase (PGP9.5), markers for defined subsets of mature taste cells, to demonstrate that liposome-mediated transfection targets multiple taste cell types. After testing eight commercially available lipids, we identified one, Transfast, that is most effective on taste cells. We also demonstrate the effectiveness of two common "promiscuous" promoters and one promoter that taste cells use endogenously. These studies should permit ex vivo strategies for studying development and cellular function in taste cells.
Modulation of humoral immune response to influenza vaccines by BCG. (1985)

BCG-induced modulation of humoral immune response to influenza vaccines was studied on adult volunteers and laboratory animals. Three influenza vaccine types were used: inactivated whole-virion vaccine prepared by chromatography, chemically split adsorbed vaccine and live allantoic vaccine for intranasal vaccination. According to the indices of humoral immunity against influenza such as seroconversion, frequency and intensity of antibody formation and degree of protection to subsequent administration of the vaccine virus, the BCG vaccine had a marked immunomodulating effect in combination with the adsorbed chemically split vaccine. The effect was slight in the case of the whole-virion vaccine and was absent with the live attenuated vaccine.
Zigzag decoding (2008)

This paper presents ZigZag, an 802.11 receiver design that combats hidden terminals. ZigZag's core contribution is a new form of interference cancellation that exploits asynchrony across successive collisions. Specifically, 802.11 retransmissions, in the case of hidden terminals, cause successive collisions. These collisions have different interference-free stretches at their start, which ZigZag exploits to bootstrap its decoding.
Heat Shock Protein 90α–Dependent Translocation of Annexin II to the Surface of Endothelial Cells Modulates Plasmin Activity in the Diabetic Rat Aorta (2004)

The goals of this article were (1) to identify cell surface proteins whose expression was regulated by diabetes and (2) to assess their contribution to diabetic complications. We purified heat shock protein 90alpha (Hsp90alpha) from the membrane fraction of high glucose-treated endothelial cells (ECs) as a binding partner for a diabetes-specific phage. Further investigation revealed that high glucose elevated cell surface Hsp90alpha in cultured cells, and that diabetes increased the amount of Hsp90alpha on the luminal surface of the aorta. We also found that high glucose or diabetes promoted the association of Hsp90alpha with annexin II and increased the expression of annexin II on the surface of aortic ECs. Finally, plasmin activity was increased by high glucose or diabetes, and this change was partially reversed with an annexin II antibody. These findings reveal a novel glucose-regulated interaction between Hsp90alpha and annexin II, and raise the possibility that increased expression of annexin II, which promotes the generation of plasmin, is linked to clotting abnormalities associated with the diabetic state.
RNA editing blood biomarkers for predicting mood alterations in HCV patients (2019)

Treatment-emergent depression is a common complication in patients with chronic hepatitis C virus (HCV) infection undergoing antiviral combination therapy with IFN-α and ribavirin. It has recently been shown that changes in A-to-I RNA editing rates are associated with various pathologies such as inflammatory disorders, depression and suicide. Interestingly, IFN-α induces gene expression of the RNA editing enzyme ADAR1-1 (ADAR1a-p150) and alters overall RNA editing activity. In this study, we took advantage of the high prevalence of pharmacologically induced depression in patients treated with IFN-α and ribavirin to test the interest of RNA editing-related biomarkers in white blood cells of patients. In this 16-week longitudinal study, a small cohort of patients was clinically evaluated using standard assessment methods prior to and during antiviral therapy and blood samples were collected to analyse RNA editing modifications. A-I RNA editing activity on the phosphodiesterase 8A (PDE8A) gene, a previously identified RNA editing hotspot in the context of lupus erythematosus, was quantified by using an ultra-deep next-generation sequencing approach. We also monitored gene expression levels of the ADAR enzymes and the PDE8A gene during treatment by qPCR. As expected, psychiatric evaluation could track treatment-emergent depression, which occurred in 30% of HCV patients. We show that PDE8A RNA editing is increased in all patients following interferon treatment, but differently in 30% of patients. This effect was mimicked in a cellular model using SHSY-5Y neuroblastoma cells. By combining the data of A-I RNA editing and gene expression, we generated an algorithm that allowed discrimination between the group of patients who developed a treatment-emergent depression and those who did not. The current model of drug-induced depression identified A-I RNA editing biomarkers as useful tools for the identification of individuals at risk of developing depression in an objective, quantifiable biological blood test.
Ringschlußmetathese von funktionalisierten Acetylen-Derivaten: ein neuer Weg zu Cycloalkinen (1998)

Ein Wolfram-Alkylidin-Katalysator ermöglichte die ersten Ringschlußmetathesen von Diinen zu funktionalisierten Makrocyclen [Gl. (a)]. Durch Reduktion der entstehenden Cycloalkine erhält man Cycloalkene mit (Z)-konfigurierter Doppelbindung, die durch Alkenmetathese zur Zeit nicht stereoselektiv synthetisiert werden können.
Room temperature study of the optical switching of a spin crossover compound inside its thermal hysteresis loop (2010)

We have studied the low-spin to high spin state phase transition induced by a single or a sequence of nanosecond laser pulses within the thermal hysteresis loop of the [Fe(NH2-trz)3](NO3)2-H2O spin crossover compound. We demonstrate that the final state that is photoinduced can be finely controlled by changing the central wavelength and the energy of the laser pulses. A simple model accounts for the observed phenomena and paves the way for the practical applications to optical data storage at room temperature of spin state transition compounds.
Geometric fracture modeling in Bolt (2009)

Modeling the geometry of solid materials cracking and shattering into elaborately shaped pieces is a painstaking task, which is often impractical to tune by hand when a large number of fragments are produced. In Walt Disney's animated feature film Bolt, cracking and shattering objects were prominent visual elements in a number of action sequences. We designed a system to facilitate the modeling of cracked and shattered objects, enabling the automatic generation of a large number of fragments while retaining the flexibility to artistically control the density and complexity of the crack formation, or even manually controlling the shape of the resulting pieces where necessary. Our method resolves every fragment exactly into a separate triangulated surface mesh, producing pieces that line up perfectly even upon close inspection, and allows straightforward transfer of texture and look properties from the un-fractured model.
Vector-based elastic reverse time migration based on scalar imaging condition (2017)

The scalar images (PP, PS, SP, and SS) of elastic reverse time migration (ERTM) can be generated by applying an imaging condition as crosscorrelation of pure wave modes. In conventional ERTM, Helmholtz decomposition is commonly applied in wavefield separation, which leads to a polarity reversal problem in converted-wave images because of the opposite polarity distributions of the S-wavefields. Polarity reversal of the converted-wave image will cause destructive interference when stacking over multiple shots. Besides, in the 3D case, the curl calculation generates a vector S-wave, which makes it impossible to produce scalar PS, SP, and SS images with the crosscorrelation imaging condition. We evaluate a vector-based ERTM (VB-ERTM) method to address these problems. In VB-ERTM, an amplitude-preserved wavefield separation method based on decoupled elastic wave equation is exploited to obtain the pure wave modes. The output separated wavefields are both vectorial. To obtain the scalar images, the scalar imaging condition in which the scalar product of two vector wavefields with source-normalized illumination is exploited to produce scalar images instead of correlating Cartesian components or magnitude of the vector P- and S-wave modes. Compared with alternative methods for correcting the polarity reversal of PS and SP images, our ERTM solution is more stable and simple. Besides these four scalar images, the VB-ERTM method generates another PP-mode image by using the auxiliary stress wavefields. Several 2D and 3D numerical examples are evaluated to demonstrate the potential of our ERTM method.
Internet security (1997)

article Free Access Share on Internet security: firewalls and beyond Author: Rolf Oppliger Swiss Federal Agency for Information Technology and Systems Swiss Federal Agency for Information Technology and SystemsView Profile Authors Info & Claims Communications of the ACMVolume 40Issue 5May 1997 pp 92–102https://doi.org/10.1145/253769.253802Published:01 May 1997Publication History 88citation10,710DownloadsMetricsTotal Citations88Total Downloads10,710Last 12 Months359Last 6 weeks117 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my Alerts New Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
Multiple model-based control: development and initial evaluation (2003)

For a large class of physical systems the structure of the plant dynamics is known, but the parameters in the system model vary with time and/or configuration. A new form of adaptive model-based control has been developed for this class of control problem. The multiple model-based control (MMBC) technique utilizes knowledge of nominal plant dynamics and principles of Bayesian estimation to provide controller performance independent of plant parameter variations. The MMBC is formed by augmenting a model-based controller with a new closed-loop form of multiple model adaptive estimation ( Delta MMAE). The Delta MMAE uses perturbation models of the combined plant and feedback control system, along with measurements of tracking error, to provide an estimate of the plant parameters. The model-based controller combines the a priori knowledge of plant structure with the parameter estimates to produce the multiple models of the plant dynamics required to maintain controller accuracy. Extensive simulation studies have demonstrated the MMBC's ability to adapt quickly and accurately adapt to the parameter variations of a plant described by complex nonlinear coupled dynamics.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Site‐specific experimental metastasis patterns of two human breast cancer cell lines in nude rats (1999)

Animal models for breast cancer metastasis are valuable tools for studying mechanisms of metastasis and for preclinical testing of anti-metastatic therapy regimens. Using MA-11 and MT-1, two oestrogen and progesterone receptor-negative human breast cancer cell lines, we developed new models in nude rats with patterns of experimental metastasis resembling those frequently observed in humans. MA-11 cells showed a clear preference for growth in the CNS. Fourteen of 15 animals injected with MA-11 cells into the left ventricle of the heart developed hind-leg paralysis, and tumours were observed in the spinal cord. MT-1 cells consistently exhibited bone/bone marrow metastases after intracardial injection, in addition to tumours in the brain and spinal cord. When injected into the cisterna magna, both cell lines gave rise to leptomeningeal neoplastic disease. Injection of MA-11 cells into the tibial bone marrow resulted in tumours in only 2 of 13 rats, whereas all animals injected with MT-1 cells developed tumours. Only 2 of 6 rats injected i.v. with MA-11 cells developed lung colonies compared with all 9 animals injected with MT-1 cells. Cell-surface expression of the following was examined: EGP2; MUC1; EGFr; E- and N-cadherin; the alpha2, alpha3, alpha5, beta1 and beta4 integrins; c-erb-B2; and N-CAM. c-erb-B2 was expressed in a higher percentage of the bone-metastasizing MT-1 cells than the MA-11 cells, whereas E-cadherin was expressed in MA-11 but not MT-1 cells. In animals injected with MA-11 and MT-1 cells in the left cardiac ventricle, treatment with cisplatin and doxorubicin did not improve survival. In summary, these clinically relevant animal models may be used for studies related to site-specific growth and metastasis and for assessing effects of experimental therapy against human breast cancer.
Learning laparoscopic video shot classification for gynecological surgery (2017)

Videos of endoscopic surgery are used for education of medical experts, analysis in medical research, and documentation for everyday clinical life. Hand-crafted image descriptors lack the capabilities of a semantic classification of surgical actions and video shots of anatomical structures. In this work, we investigate how well single-frame convolutional neural networks (CNN) for semantic shot classification in gynecologic surgery work. Together with medical experts, we manually annotate hours of raw endoscopic gynecologic surgery videos showing endometriosis treatment and myoma resection of over 100 patients. The cleaned ground truth dataset comprises 9 h of annotated video material (from 111 different recordings). We use the well-known CNN architectures AlexNet and GoogLeNet and train these architectures for both, surgical actions and anatomy, from scratch. Furthermore, we extract high-level features from AlexNet with weights from a pre-trained model from the Caffe model zoo and feed them to an SVM classifier. Our evaluation shows that we reach an average recall of .697 and .515 for classification of anatomical structures and surgical actions respectively using off-the-shelf CNN features. Using GoogLeNet, we achieve a mean recall of .782 and .617 for classification of anatomical structures and surgical actions respectively. With AlexNet the achieved recall is .615 for anatomical structures and .469 for surgical action classification respectively. The main conclusion of our work is that advances in general image classification methods transfer to the domain of endoscopic surgery videos in gynecology. This is relevant as this domain is different from natural images, e.g. it is distinguished by smoke, reflections, or a limited amount of colors.
Activity of Bacteriophages in Murine Tumor Models Depends on the Route of Phage Administration (2005)

Previously we investigated the anticancer activity of bacteriophage preparations in various murine tumor models. We demonstrated the antimetastatic activity of purified and nonpurified bacteriophage preparations injected intraperitoneally (IP). However, in solid tumors we observed antitumor activity of purified bacteriophages, but the lysates (raw preparations obtained by culturing phages with bacteria) stimulated tumor growth. In this article we present a comparison of the antitumor activity of bacteriophages after oral (per os, PO) and IP administration of lysates and purified preparations. Our observations indicate that PO application of a bacteriophage preparation is safer and at least as effective as IP. Stimulation of solid tumors by lysates administered orally was not observed, and bacteriophages applied PO were more effective in inhibiting metastases formation. These observations are of great importance in any consideration of possible therapeutic applications of bacteriophages. The role of the route of bacteriophage administration should be considered in the context of the effectiveness and safety of such therapies.
Benchmarking Keyword Spotting Efficiency on Neuromorphic Hardware (2019)

Using Intel's Loihi neuromorphic research chip and ABR's Nengo Deep Learning toolkit, we analyze the inference speed, dynamic power consumption, and energy cost per inference of a two-layer neural network keyword spotter trained to recognize a single phrase. We perform comparative analyses of this keyword spotter running on more conventional hardware devices including a CPU, a GPU, Nvidia's Jetson TX1, and the Movidius Neural Compute Stick. Our results indicate that for this real-time inference application, Loihi outperforms all of these alternatives on an energy cost per inference basis while maintaining equivalent inference accuracy. Furthermore, an analysis of tradeoffs between network size, inference speed, and energy cost indicates that Loihi's comparative advantage over other low-power computing devices improves for larger networks.
A geometric modeling tool for visualization of human anatomical structures (2002)

A geometric modeling system for creating and visualizing models of human anatomical structures has been developed. Many structures in the human body such as blood vessels, bones, the breast, and the chest cavity can be naturally modeled using generalized cylinder and quadric primitives. These models can be accurately and efficiently rendered to form simulated transmitted X-ray and reflected visible light images of human anatomy. Stereoscopic images can be easily generated to allow 3D viewing. Representation of organs by geometric models allows easy modification of their size, shape, and grouping relations. Rotation and viewing in arbitrary direction, and anatomical manipulations such as removal of organs to reveal details of surrounding structures, can be accomplished. Some potential applications and sample images generated by the authors' system are presented.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
On-axis near-eye display system based on directional scattering holographic waveguide and curved goggle (2019)

The contradiction between the field of view (FOV), luminance uniformity (LU) and weight has always restricted the development of augmented reality display systems. An on-axis near-eye display (NED) system based on directional scattering holographic waveguide (DSHW) and curved goggle is proposed in order to realize a large FOV with high LU, light weight, and conformal design capability. The DSHW which consists of a linear volume holographic grating and holographic diffuser is used to deliver the virtual image and construct a transparent directional emission display screen with high LU. The curved goggle is used to project the image on the display screen into human eye and form a large FOV, with a suitable exit pupil diameter (EPD) and eye relief distance (ERF) and while keeping the external scene visible. Our proposed NED achieved an FOV of 44° horizontal (H) × 12° vertical (V) easily, which is almost consistent with the theoretical design. The EPD is 6 mm, ERF is 18.6 mm, and LU is about 88.09% at full viewing angle. The system is lightweight and flexible, which can be further applied in the next-generation, integrated protection-display helmet system through conformal optical design.
Musashi, a neural RNA-binding protein required for drosophila adult external sensory organ development (1994)

A family of neural RNA-binding proteins has recently been described in both vertebrates and invertebrates. We have identified a new member of this family, the Drosophila musashi (msi) locus, which is required for development of adult external sensory organs (sensilla). In contrast with wild-type sensilla, which contain two outer support cells, the msi mutation typically results in the appearance of extra outer support cells. The msi putative RNA-binding protein is localized to the nucleus and appears to be expressed in all cells in each sensillum and predominantly in neurons during embryogenesis. We propose that the msi protein regulates sensillum development by controlling the expression of target genes at the posttranscriptional level.
Reversible enantiofaciale Differenzierung eines einzelnen heterocyclischen Substrates durch supramolekulare Rezeptoren (2003)

Wo oben und unten ist: Supramolekulare Rezeptoren können zwischen der „Ober“- und „Unter“-Seite eines einzelnen Coffein-Gastmoleküls unterscheiden (siehe Schema). Diese reversible enantiofaciale Differenzierung führt zur jeweils bevorzugten Bildung von α- oder β-Komplexen.
Modified Masson Trichrome v1 (2017)

Automated fibrosis analysis using Orbit module, is based on this modified masson trichrome staining protocol
Black‐box identification of mimo transfer functions: Asymptotic properties of prediction error models (1989)

Abstract Identification of multi‐input/multi‐output (MIMO) transfer functions is considered. The transfer function matrix is parametrized as black‐box models which have certain shift properties; no structure or order is chosen a priori. In order to obtain a good transfer function estimate, we allow the order of the model to increase to infinity as the number of data tends to infinity. The expression of asymptotic covariance of the transfer function estimates is derived, which is asymptotic both in the number of data and in the model order. The result indicates that the joint covariance matrix of the transfer function estimates of the process and of the noise filter is proportional to the (generalized) ratio of output noise to imput signal; the factor of proportionality is the ratio of model order to number of data. The result is independent of the particular model structure used. This result is the MIMO extension of the theory of Ljung. The application of this theory for defining the upper bounds of identification errors is highlighted.
Effect of pH on the Radical Quenching Capacity of Tea Infusions Using the ABTS•+ Assay (2016)

The aims of this study were to assess the impact of pH on the free radical quenching activity of tea infusions using a modified 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS) assay and three antioxidant compounds as reference. Study Design: In-vitro method.
High-speed metal-semiconductor-metal photodetectors on InP:Fe (1990)

Metal-semiconductor-metal (MSM) photodetectors on semi-insulating InP:Fe with a lateral planar structure have been fabricated. Laterally structured photodiodes are formed with interdigitated contact fingers. The detectors exhibit low dark currents of about 10 nA, an impulse response of 26 ps FWHM (full-width of half-maximum), and an internal quantum efficiency of 80%, all at 10 V bias. An electrical cutoff frequency of 40 GHz for the packaged detectors is obtained from s-parameter measurements, demonstrating that the response time is not limited by the parasitic elements of the devices.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>