gebhart/patrepeval huggingface dataset:
SU‐HH‐BRB‐02: A Study on the Focusing Power of Dynamic Photon Painting (2010)

Purpose : Cross‐firing technique is widely used in photon‐based radiosurgery to create sharp dose falloff near the target. In this technique, a large number of beams are focused to the isocenter in order to minimize contribution from individual beams. In this study, we investigated a technique called dynamic photon painting (DPP), where a radiation beam changed dynamically in direction to irradiate a target, to significantly enhance dose falloff rate. The dynamic motion of the beam source is equivalent to focusing tens of thousands of beams, creating a sharp dose gradient that potentially rival proton Bragg peaks. Materials and Methods : To study DPP, we took a Cybeknife cone beam and rotate it in a hemispherical trajectory to create DPP dose kernels. The parameters determining a kernel include: (1) range of latitude angles from 0 to 90 degrees, (2) range of longitude angles from 0 to 360 degrees, and (3) source to axis distance (SAD). Using these dose kernels, a complex 3D dose distribution can be created via traversing or “painting” a kernel inside a 3D target volume. Results : We found: (1) The dose gradient of DPP kernels rivals that of proton Bragg peaks. (2) As the latitude angular range increases, the dose gradient of DPP kernel increases in the plane defined by the circle of latitude and decreases in the plane defined by the circle of longitude. (3) As the starting latitude angle increases, the dose distribution of low dose regions becomes less regular in the plane of the circle of longitude. (4) When DPP kernels are used in treatment planning via dose painting, we get better plans than Gamma Knife radiosurgery. Conclusions : We have demonstrated that Dynamic Photon Painting has the potential to rival proton therapy and outperform Gamma Knife radiosurgery for our studied cases.
Novel Virtual Screening Approach for the Discovery of Human Tyrosinase Inhibitors (2014)

Tyrosinase is the key enzyme involved in the human pigmentation process, as well as the undesired browning of fruits and vegetables. Compounds inhibiting tyrosinase catalytic activity are an important class of cosmetic and dermatological agents which show high potential as depigmentation agents used for skin lightening. The multi-step protocol employed for the identification of novel tyrosinase inhibitors incorporated the Shape Signatures computational algorithm for rapid screening of chemical libraries. This algorithm converts the size and shape of a molecule, as well its surface charge distribution and other bio-relevant properties, into compact histograms (signatures) that lend themselves to rapid comparison between molecules. Shape Signatures excels at scaffold hopping across different chemical families, which enables identification of new actives whose molecular structure is distinct from other known actives. Using this approach, we identified a novel class of depigmentation agents that demonstrated promise for skin lightening product development.
ProxiMate (2011)

Forming secure associations between wireless devices that do not share a prior trust relationship is an important problem. This paper presents ProxiMate, a system that allows wireless devices in proximity to securely pair with one another autonomously by generating a common cryptographic key directly from their shared time-varying wireless environment. The shared key synthesized by ProxiMate can be used by the devices to authenticate each others' physical proximity and then to communicate confidentially. Unlike traditional pairing approaches such as Diffie-Hellman, ProxiMate is secure against a computationally unbounded adversary and its computational complexity is linear in the size of the key. We evaluate ProxiMate using an experimental prototype built using an open-source software-defined platform and demonstrate its effectiveness in generating common secret bits. We further show that it is possible to speed up secret key synthesis by monitoring multiple RF sources simultaneously or by shaking together the devices that need to be paired. Finally, we show that ProxiMate is resistant to even the most powerful attacker who controls the public RF source used by the legitimate devices for pairing.
Biomolecular Template‐Induced Biomimetic Coating of Hydroxyapatite on an SS 316 L Substrate (2007)

Bone, a natural composite, comprises non‐stoichiometric calcium hydroxyapatite (HAp) precipitated in a controlled reaction environment of a highly aligned, anisotropic organic template (type I collagen) that leads to its exotic tensile and compressive strength. It differs from stoichiometric hydroxyapatite in composition, crystallinity, and other physical and mechanical properties. In the present study, functionalized biomolecular template‐induced precipitation of HAp on an SS 316 L substrate following biomimetic route exhibits distinct alterations in crystal growth and geometry, which in turn indicates the potential of the process to develop a non‐stoichiometric HAp coating on metal implants.
Compiler transformations for effectively exploiting a zero overhead loop buffer (2004)

Abstract A Zero Overhead Loop Buffer (ZOLB) is an architectural feature that is commonly found in DSP processors. This buffer can be viewed as a compiler managed cache that contains a sequence of instructions that will be executed a specified number of times without incurring any loop overhead. Unlike loop unrolling, a loop buffer can be used to minimize loop overhead without the penalty of increasing code size. In addition, a ZOLB requires relatively little space and power, which are both important considerations for most DSP applications. This paper describes strategies for generating code to effectively use a ZOLB. We have found that many common code improving transformations used by optimizing compilers on conventional architectures can be easily used to (1) allow more loops to be placed in a ZOLB, (2) further reduce loop overhead of the loops placed in a ZOLB, and (3) avoid redundant loading of ZOLB loops. The results given in this paper demonstrate that this architectural feature can often be exploited with substantial improvements in execution time and slight reductions in code size for various signal processing applications. Copyright © 2004 John Wiley & Sons, Ltd.
The Relevance of Large Strains in Functional Tissue Engineering of Heart Valves (2003)

The results demonstrate the significance of large strains in promoting tissue formation. This study may provide a methodological basis for tissue engineering of heart valves appropriate for systemic pressure applications.
Alginate/lactose‐modified chitosan hydrogels: A bioactive biomaterial for chondrocyte encapsulation (2007)

A new bioactive scaffold was prepared from a binary polysaccharide mixture composed of a polyanion (alginate) and a polycation (a lactose-modified chitosan, chitlac). Its potential use for articular chondrocytes encapsulation and cartilage reconstructive surgery applications has been studied. The hydrogel combines the ability of alginate to act as a 3D supporting structure with the capability of the second component (chitlac) to provide interactions with porcine articular chondrocytes. Physico-chemical characterization of the scaffold was accomplished by gel kinetics and compression measurements and demonstrated that alginate-chitlac mixture (AC-mixture) hydrogels exhibit better mechanical properties when compared with sole alginate hydrogels. Furthermore, biochemical and biological studies showed that these 3D scaffolds are able to maintain chondrocyte phenotype and particularly to significantly stimulate and promote chondrocyte growth and proliferation. In conclusion, the present study can be considered as a first step towards an engineered, biologically active scaffold for chondrocyte in vitro cultivation, expansion, and cell delivery.
Biological Activity of Cryopreserved Bovine Spermatogonial Stem Cells During In Vitro Culture1 (2004)

Functional roles of spermatogonial stem cells in spermatogenesis are self-renewing proliferation and production of differentiated daughter progeny. The ability to recapitulate these actions in vitro is important for investigating their biology and inducing genetic modification that could potentially lead to an alternative means of generating transgenic animals. The objective of this study was to evaluate the survival and proliferation of frozen-thawed bovine spermatogonial stem cells in vitro and investigate the effects of exogenous glial cell line-derived neurotrophic factor (GDNF). In order to accomplish this objective we developed a bovine embryonic fibroblast feeder cell line, termed BEF, to serve as feeder cells in a coculture system with bovine germ cells. Bovine spermatogonial stem cell survival and proliferation in vitro were evaluated by xenogeneic transplantation into the seminiferous tubules of immunodeficient mice. Bovine germ cells cocultured for 1 wk resulted in significantly more round cell donor colonies in recipient mouse testes compared to donor cells transplanted just after thawing. Bovine germ cells cocultured for 2 wk had fewer colony-forming cells than the freshly thawed cell suspensions or cells cultured for 1 wk. Characterization of the feeder cell line revealed endogenous expression of Gdnf mRNA and protein. Addition of exogenous GDNF to the culture medium decreased the number of stem cells present at 1 wk of coculture, but enhanced stem cell maintenance at 2 wk compared to cultures without added GDNF. These data indicate that frozen-thawed bovine spermatogonial stem cells survive cryopreservation and can be maintained during coculture with a feeder cell line in which the maintenance is influenced by GDNF.
Anti-ACVR1 antibodies exacerbate heterotopic ossification in fibrodysplasia ossificans progressiva (FOP) by activating FOP-mutant ACVR1 (2022)

Fibrodysplasia ossificans progressiva (FOP) is a rare genetic disorder whose most debilitating pathology is progressive and cumulative heterotopic ossification (HO) of skeletal muscles, ligaments, tendons, and fascia. FOP is caused by mutations in the type I BMP receptor gene ACVR1, which enable ACVR1 to utilize its natural antagonist, activin A, as an agonistic ligand. The physiological relevance of this property is underscored by the fact that HO in FOP is exquisitely dependent on activation of FOP-mutant ACVR1 by activin A, an effect countered by inhibition of anti-activin A via monoclonal antibody treatment. Hence, we surmised that anti-ACVR1 antibodies that block activation of ACVR1 by ligands should also inhibit HO in FOP and provide an additional therapeutic option for this condition. Therefore, we generated anti-ACVR1 monoclonal antibodies that block ACVR1's activation by its ligands. Surprisingly, in vivo, these anti-ACVR1 antibodies stimulated HO and activated signaling of FOP-mutant ACVR1. This property was restricted to FOP-mutant ACVR1 and resulted from anti-ACVR1 antibody-mediated dimerization of ACVR1. Conversely, wild-type ACVR1 was inhibited by anti-ACVR1 antibodies. These results uncover an additional property of FOP-mutant ACVR1 and indicate that anti-ACVR1 antibodies should not be considered as therapeutics for FOP.
Microstructure Control of Barium Titanate - Potassium Niobate Solid Solution System Ceramics by MPB Engineering and Their Piezoelectric Properties (2011)

KBaTiNbO6, i.e., 0.5BT-0.5KN complete solid solution ceramics and barium titanate (BaTiO3, BT) - potassium niobate (KNbO3, KN) system (0.5BT-0.5KN) ceramics with various microstructures were prepared by conventional sintering method and two-step sintering method. The 0.5BT-0.5KN with diffusion of BT and KN completely were paraelectrics. Their microstructures were investigated using X-ray diffraction (XRD) measurement and scanning electron microscopy (SEM). KBaTiNbO6 at room temperature was assigned to cubic Pm3m symmetry. For the 0.5BT-0.5KN patially solid solution system ceramics, the control of the interface area between two phases was important to enhance piezoelectric property. Their piezoelectric property was measured, and the apparent piezoelectric constant d33* increased with increasing interface area.
Surface-tailoring of emulsomes for boosting brain delivery of vinpocetine via intranasal route: <i>in vitro</i> optimization and <i>in vivo</i> pharmacokinetic assessment (2022)

Vinpocetine (VNP), a semisynthetic active pharmaceutical ingredient, is used for oral management of cerebrovascular diseases because of its ability to enhance the blood flow to the brain. However, despite that, the therapeutic application of VNP is restricted due to its reduced bioavailability and diminished brain levels that could be attributed to its low aqueous solubility, short half-life, and presystemic metabolism exposure. Accordingly, the goal of this work was to explore the ability of surface-tailored intranasal emulsomes to boost brain delivery of the drug. A 3<sup>2</sup>2<sup>1</sup> factorial design was implemented to explore the impact of phospholipid (PL) to solid lipid weight ratio, PL to cholesterol molar ratio, and type of solid lipid on vesicle size, zeta potential, drug entrapment, and release efficiency of the new developed VNP emulsomes. Tailoring of the optimized emulsomal surface formulation was performed using either cationization or PEGylation approaches to boost blood-brain barrier penetration. The pharmacokinetic assessment in rats showed significantly improved bioavailability of VNP emulsomal formulations compared to the oral market product. Additionally, surface-tailored emulsomes exhibited significantly higher brain levels compared to the optimized emulsomes. Based on these findings, the proposed surface-tailored emulsomes could be considered as a promising platform for achieving high brain levels of VNP following intranasal administration.
Recent innovations in cost-effective polymer and paper hybrid microfluidic devices (2021)

Hybrid microfluidic systems that are composed of multiple different types of substrates have been recognized as a versatile and superior platform, which can draw benefits from different substrates while avoiding their limitations. This review article introduces the recent innovations of different types of low-cost hybrid microfluidic devices, particularly focusing on cost-effective polymer- and paper-based hybrid microfluidic devices. In this article, the fabrication of these hybrid microfluidic devices is briefly described and summarized. We then highlight various hybrid microfluidic systems, including polydimethylsiloxane (PDMS)-based, thermoplastic-based, paper/polymer hybrid systems, as well as other emerging hybrid systems (such as thread-based). The special benefits of using these hybrid systems have been summarized accordingly. A broad range of biological and biomedical applications using these hybrid microfluidic devices are discussed in detail, including nucleic acid analysis, protein analysis, cellular analysis, 3D cell culture, organ-on-a-chip, and tissue engineering. The perspective trends of hybrid microfluidic systems involving the improvement of fabrication techniques and broader applications are also discussed at the end of the review.
Inhibition of human immunodeficiency virus-associated reverse transcriptase by 14-O-acyladriamycins. (1989)

It was reported that doxorubicin inhibited virus reverse transcriptase. We have synthesized various new 14-O-acyladriamycins. The derivatives having a 17-O-benzoyl group with hydrophobic aliphatic side chains showed stronger inhibitory activity on human immunodeficiency virus reverse transcriptase and lower cytotoxicity on mouse leukemia cells.
MR Imaging–based Multimodal Autoidentification of Perivascular Spaces (mMAPS): Automated Morphologic Segmentation of Enlarged Perivascular Spaces at Clinical Field Strength (2017)

Purpose To describe a fully automated segmentation method that yields object-based morphologic estimates of enlarged perivascular spaces (ePVSs) in clinical-field-strength (3.0-T) magnetic resonance (MR) imaging data. Materials and Methods In this HIPAA-compliant study, MR imaging data were obtained with a 3.0-T MR imager in research participants without dementia (mean age, 85.3 years; range, 70.4-101.2 years) who had given written informed consent. This method is built on (a) relative normalized white matter, ventricular and cortical signal intensities within T1-weighted, fluid-attenuated inversion recovery, T2-weighted, and proton density data and (b) morphologic (width, volume, linearity) characterization of each resultant cluster. Visual rating was performed by three raters, including one neuroradiologist, after established single-section guidelines. Correlations between visual counts and automated counts, as well session-to-session correlation of counts within each participant, were assessed with the Pearson correlation coefficient r. Results There was a significant correlation between counts by visual raters and automated detection of ePVSs in the same section (r = 0.65, P < .001; r = 0.69, P < .001; and r = 0.54, P < .01 for raters 1, 2, and 3, respectively). With regard to visual ratings and whole-brain count consistency, average visual rating scores were highly correlated with automated detection of total burden volume (r = 0.58, P < .01) and total ePVS number (r = 0.76, P < .01). Morphology of clusters across 28 data sets was consistent with published radiographic estimates of ePVS; mean width of clusters segmented was 3.12 mm (range, 1.7-13.5 mm). Conclusion This MR imaging-based method for multimodal autoidentification of perivascular spaces yields individual whole-brain morphologic characterization of ePVS in clinical MR imaging data and is an important tool in the detailed assessment of these features. <sup>©</sup> RSNA, 2017 Online supplemental material is available for this article.
Interband cascade laser on silicon (2017)

We demonstrate the first interband cascade lasers heterogeneously integrated with silicon waveguides. The 3.6 μm wavelength lasers operate in pulsed mode at room temperature, with threshold currents as low as 394 mA.
The Nuclear Factor of Activated T Cells (Nfat) Transcription Factor Nfatp (Nfatc2) Is a Repressor of Chondrogenesis (2000)

Nuclear factor of activated T cells (NFAT) transcription factors regulate gene expression in lymphocytes and control cardiac valve formation. Here, we report that NFATp regulates chondrogenesis in the adult animal. In mice lacking NFATp, resident cells in the extraarticular connective tissues spontaneously differentiate to cartilage. These cartilage cells progressively differentiate and the tissue undergoes endochondral ossification, recapitulating the development of endochondral bone. Proliferation of already existing articular cartilage cells also occurs in some older animals. At both sites, neoplastic changes in the cartilage cells occur. Consistent with these data, NFATp expression is regulated in mesenchymal stem cells induced to differentiate along a chondrogenic pathway. Lack of NFATp in articular cartilage cells results in increased expression of cartilage markers, whereas overexpression of NFATp in cartilage cell lines extinguishes the cartilage phenotype. Thus, NFATp is a repressor of cartilage cell growth and differentiation and also has the properties of a tumor suppressor.
Spin stabilized magnetic levitation of horizontal rotors. (2004)

In this paper we present an analysis of a new configuration for achieving spin stabilized magnetic levitation. In the classical configuration, the rotor spins about a vertical axis; and the spin stabilizes the lateral instability of the top in the magnetic field. In this new configuration the rotor spins about a horizontal axis; and the spin stabilizes the axial instability of the top in the magnetic field.
Structure and Dynamics of Meprin β in Complex with a Hydroxamate-Based Inhibitor (2021)

The astacin protease Meprin β represents an emerging target for drug development due to its potential involvement in disorders such as acute and chronic kidney injury and fibrosis. Here, we elaborate on the structural basis of inhibition by a specific Meprin β inhibitor. Our analysis of the crystal structure suggests different binding modes of the inhibitor to the active site. This flexibility is caused, at least in part, by movement of the C-terminal region of the protease domain (CTD). The CTD movement narrows the active site cleft upon inhibitor binding. Compared with other astacin proteases, among these the highly homologous isoenzyme Meprin α, differences in the subsites account for the unique selectivity of the inhibitor. Although the inhibitor shows substantial flexibility in orientation within the active site, the structural data as well as binding analyses, including molecular dynamics simulations, support a contribution of electrostatic interactions, presumably by arginine residues, to binding and specificity. Collectively, the results presented here and previously support an induced fit and substantial movement of the CTD upon ligand binding and, possibly, during catalysis. To the best of our knowledge, we here present the first structure of a Meprin β holoenzyme containing a zinc ion and a specific inhibitor bound to the active site. The structural data will guide rational drug design and the discovery of highly potent Meprin inhibitors.
Isolated broadband attosecond pulse generation with near- and mid-infrared driver pulses via time-gated phase matching (2017)

We present a theoretical analysis of the time-gated phase matching (ionization gating) mechanism in high-order harmonic generation for the isolation of attosecond pulses at near-infrared and mid-infrared driver wavelengths, for both few-cycle and multi-cycle driving laser pulses. Results of our high harmonic generation and three-dimensional propagation simulations show that broadband isolated pulses spanning from the extreme-ultraviolet well into the soft X-ray region of the spectrum can be generated for both few-cycle and multi-cycle laser pulses. We demonstrate the key role of absorption and group velocity matching for generating bright, isolated, attosecond pulses using long wavelength multi-cycle pulses. Finally, we show that this technique is robust against carrier-envelope phase and peak intensity variations.