gebhart/patrepeval huggingface dataset:
Evaluation of the Aboriginal Relationship and Cultural Competency Courses among a sample of Indigenous Services Canada nurses (2019)

In 2015, Cancer Care Ontario launched the Aboriginal Relationship and Cultural Competency (ARCC) courses, which stress the importance for healthcare professionals to understand and apply First Nations, Inuit, and Métis (FNIM) cultural safety to provide effective person-centred care. The courses address a key recommendation from the Truth and Reconciliation Commission of Canada report, to provide skills-based training in cultural competency, conflict resolution, human rights and anti-racism. The objective of the evaluation is to validate the tool to assess: if the delivery mechanism is appropriate and feasible; if participants acquire an increased knowledge of the courses' contents; and if positive change in how healthcare practice is delivered is perceived to have resulted. After the ARCC courses were mandated for Indigenous Services Canada (ISC) nurses, an anonymous survey was delivered and a focus group was conducted at a regional meeting. The responses from the surveys were gathered in an excel spreadsheet for analyses and the focus group data were analyzed for key themes. All the nurses in attendance completed the survey (n=22) and a portion participated in the focus group (n=8). Our evaluation demonstrated that free, online, module formatted courses were appropriate and relevant for ISC nurses (81%); the courses increased the knowledge about FNIM people (72%); and the nurses have/will apply what they learned in their practice (82%). There has been an increasing movement for regions and organizations in Canada to complete cultural competency training. Our evaluation demonstrated that free, online, module formatted courses were successful at meeting learning objectives.
Shifts in cadherin profiles between human normal melanocytes and melanomas. (1996)

Direct contacts between keratinocytes and melanocytes play an important role in conserving the characteristic phenotype and biologic behavior of melanocytic cells. Although the mechanisms involved remain unclear, given the role of adhesion molecules in controlling cellular interactions, disturbances in normal keratinocyte-melanocyte adhesion mediated by cadherin may contribute to malignant transformation by releasing melanocytes from a variety of contact-mediated regulatory controls. To determine the potential clinical relevance of cadherin profiles in melanomas and to study their possible involvement in the phenotypic plasticity of melanocytic cells, we used immunostaining, biochemical, and co-culture techniques. Double immunofluorescence demonstrated expression of cadherins and their associating proteins, alpha- and beta-catenin, in melanocytes in situ. Melanomas were heterogeneous when evaluated immunohistochemically, with positive rates of four of 14, eight of 12, and 12 of 16 to anti-E-, anti-P-, and anti-N-cadherin monoclonal antibodies, respectively. Flow cytometry indicated abundant expression of E-cadherin but marginal P- and N-cadherin in cultured melanocytes. In contrast, only one (WM1232) of 16 melanoma cell lines tested was positive for E-cadherin, none was positive for P-cadherin, and all but one were positive for N-cadherin. Western blot confirmed E-cadherin expression in melanocytic cells. Immunoprecipitation further revealed complexes of E-cadherin with catenins in WM1232 melanoma cells. Co-culture studies indicated that only melanoma cells expressing E-cadherin (WM1232) were susceptible to keratinocyte-mediated control of the expression of the melanoma cell adhesion molecule, Mel-CAM. The results suggest downregulation of E-cadherin but upregulation of N-cadherin in melanoma cells. Such a shift in cadherin profiles may endow melanocytic cells with new adhesive properties and altered spatial relations that favor uncontrolled proliferation, migration, and invasion.
Arrhythmia in Isolated Prenatal Hearts after Ablation of the Ca<sub>v</sub>2.3 (α1E) Subunit of Voltage-gated Ca<sup>2+</sup> Channels (2004)

A voltage-gated calcium channel containing Cav2.3e (alpha1Ee) as the ion conducting pore has recently been detected in rat heart. Functional evidence for this Ca2+ channel to be involved in the regulation of heart beating, besides L- and T-type channels, was derived from murine embryos where the gene for Cav1.2 had been ablated. The remaining "L-type like" current component was not related to recombinant splice variants of Cav1.3 containing channels. As recombinant Cav2.3 channels from rat were reported to be weakly dihydropyridine sensitive, the spontaneous activity of the prenatal hearts from Cav2.3(-|-) mice was compared to that of Cav2.3(+|+) control animals to investigate if Cav2.3 could represent such a L-type like Ca(2+) channel. The spontaneous activity of murine embryonic hearts was recorded by using a multielectrode array. Between day 9.5 p.c. to 12.5 p.c., the beating frequency of isolated embryonic hearts from Cav2.3-deficient mice did not differ significantly from control mice but the coefficient of variation within individual episodes was more than four-fold increased in Cav2.3-deficient mice indicating arrhythmia. In isolated hearts from wild type mice, arrhythmia was induced by superfusion with a solution containing 200 nM SNX-482, a blocker of some R-type voltage gated Ca2+ channels, suggesting that R-type channels containing the splice variant Cav2.3e as ion conducting pore stabilize a more regular heart beat in prenatal mice.
Angiogenesis and neovascularization associated with extracellular matrix‐modified porous implants (2001)

Therapies directed toward stimulation of angiogenesis seek to accelerate the development of new blood vessels in tissues rendered dysfunctional because of an insufficient microvascular supply. The goal of the current study was the stimulation of an angiogenic response around and within porous biomedical implants, such as vascular grafts, constructed from a base polymer composed of expanded polytetrafluoroethylene (ePTFE). Similar to many biomaterials, ePTFE does not elicit a significant angiogenic response and the porous interstices of this material remain avascular after implantation. Studies were performed to evaluate the ability of a tumorigenic cell line, the 804-G rat kidney cell to secrete an angiogenic extracellular matrix on and within the porous structures of ePTFE. A rat model was used to evaluate and compare implant-associated healing responses between nonmodified materials and extracellular matrix-modified ePTFE. Results demonstrated that, in contrast to untreated ePTFE, the matrix-modified ePTFE stimulated both angiogenesis in implant-associated tissue and neovascularization of the pores within the ePTFE interstices. Deposition of an insoluble matrix stimulates an angiogenic response and has a potential application for the improvement of medical device function.
Strain on the Ulnar Nerve at the Elbow and Wrist During Throwing Motion (2005)

In the present study, the maximum strain on the ulnar nerve during the acceleration phase was found to be close to the elastic and circulatory limits of the nerve.
Work-life Balance: The Magic's in the Mix (2005)

Achieving a balanced life is a challenge without support from a firm's culture, structure and reward systems.
Intra‐ and inter‐observer reliability of the application of the cellulite severity scale to a Spanish female population (2012)

Cellulite Severity Scale has excellent reliability and internal consistency when used to evaluate cellulite on the buttocks and back of the thighs considered together. Nevertheless, the dimension grade of laxity, flaccidity or sagging skin does not contribute positively to the final consistency of the scale. This dimension needs to be analysed in greater depth in future studies.
Quantitative Determination of Ethyl Glucuronide and Ethyl Sulfate in Postmortem and Antemortem Whole Blood Using Phospholipid Removal 96-Well Plate and UHPLC–MS-MS (2020)

Postmortem ethanol formation is a well-known problem in forensic toxicology. Ethyl glucuronide (EtG) and ethyl sulfate (EtS) are ethanol metabolites that can be used to distinguish antemortem alcohol intake from postmortem formation of ethanol and in addition can be a helpful tool in assessment of the hip-flask defense. To an aliquot of 100 µL whole blood, internal standard (IS) and water was added before protein precipitation treatment (PPT) with ice-cold acetonitrile (ACN). The supernatants were filtered through a 96-well phospholipid removal plate, evaporated to dryness and reconstituted in 150 µL water/ACN/formic acid (FA). Identification of compounds was performed using multiple reaction monitoring (MRM) in negative mode. Gradient elution was performed on a C18 column with methanol (MeOH) and 0.1% FA. The run time was 4.5 min, and 0.5 µL was injected on an ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS-MS) instrument. Linearity was achieved (coefficient of determination (R2) ≥ 0.999) for EtG in the range of 0.089 to 22 mg/L (0.40-100 µM) and EtS 0.025 to 6.3 mg/L (0.20-50 µM). The limit of quantification (LOQ) was 0.067 mg/L (0.30 µM) for EtG and 0.019 mg/L (0.15 µM) for EtS. Between assay accuracy was -15% to 8% and precision reported as relative standard deviation (RSD) was ≤ 4.5%. Precision, estimated as the RSD of the concentration difference between results from two independent analyses of authentic whole blood samples, was ≤ 6.7%. Recovery was ≥ 61% for EtG and ≥ 77% for EtS and matrix effects (ME) were 99% to 103%. Method comparison was carried out with a previously used UHPLC-MS-MS method, and satisfactory agreement was achieved, and external proficiency testing control samples had z-score < ± 1. The method has been used in routine work for more than 4 years analyzing about 6,000 antemortem and postmortem whole blood samples and has proven to be robust and reliable.
Preparation of gradient copolymers via ATRP using a simultaneous reverse and normal initiation process. I. Spontaneous gradient (2005)

Abstract Spontaneous gradient copolymers were prepared in both bulk and miniemulsion systems via Atom Transfer Radical Polymerization (ATRP) utilizing a Simultaneous Reverse and Normal Initiation (SR & NI) process. Both instantaneous and cumulative compositions were used to characterize the gradient copolymers. The gradient copolymers were obtained with an array of gradient compositions ranging from a subtle to strong variation in monomer distribution along the polymer backbones, depending on the ratio of comonomers initially added to the copolymerization system. The compositions of the gradient copolymer produced in miniemulsion systems were similar to those generated in bulk. © 2005 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 43: 3616–3622, 2005
Automated cartridge system library server (2002)

A history and description of the automated cartridge system library server (ACSLS) are given. The ACSLS is a software product that introduces the StorageTek 4400 automated cartridge system (ACS) into a networked, heterogeneous computer environment. ACSLS software allows the StorageTek 4400 ACS robotic tape subsystem to be used by network-attached computer systems. There are two primary requirements for the ACSLS software: to provide portable high-level software allowing the StorageTek 4400 ACS to be used with a wide variety of computer platforms; and to design the software in layers of functionality that could serve as building blocks for future products. It is shown how these goals were achieved.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Modeling of anaerobic formate kinetics in mixed biofilm culture using dynamic membrane mass spectrometric measurement (1995)

The dynamics of the anaerobic conversion of formate in a microbial mixed culture taken from an anaerobic fluidized bed reactor was studied using a new stirred micro reactor equipped with a membrane mass spectrometer. The microreactor with a toroidally shaped bottom and pitched blade turbine and a cylindrical flow guide was thermostated and additionally equipped with a pH electrode and pH control. During fed-batch experiments using formate, the dissolved gases (methane, hydrogen, and carbon dioxide), as well as the acid consumption rates for pH control were monitored continuously. Initially and at the end of each experiment, organic acids were analyzed using ion chromatography (IC). It was found that about 50% of the formate was converted to methane via hydrogen and carbon dioxide, 40% gave methane either directly or via acetate. This was calculated from experiments using H(13)CO(3) (-) pulses and measurement of (12)CH(4) and (13)CH(4) production rates. About 10% of the formate was converted to lactate, acetate, and propionate, thereby increasing the measured CO(2)/CH(4) production ratio. The nondissociated formic acid was shown to be rate determining. From the relatively high K(s) value of 2.5 mmol m(-3), it was concluded that formate cannot play an important role in electron transfer. During dynamic feeding of formate, hydrogen concentration always increased to a maximum before decreasing again. This peak was found to be very discriminative during modeling. From the various models set up, only those with two-stage degradation and double Monod kinetics, both for CO(2) and hydrogen, were able to describe the experimental data adequately. Additional discrimination was possible with the IC measurement of organic acids. (c) 1995 John Wiley & Sons, Inc.
Ontology Based on Electronic Payment Fraud Prevention (2018)

Fraud monitoring systems are a preventive process that trigger and evaluate the overall payment activity to detect suspicious and fraudulent transactions. These systems provide at any time an overall dashboard of electronic payment risks processed by the system. Ontology is the structured set of terms and concepts representing the meaning of an information field. This paper proposes an approach able to detect and prevent suspicious transactions on various electronic payment systems using an ontology. the approach make to share and adaptive the preventives rules of fraud on an ontology based on multiple and various payment systems.
Potential Benefits Of Delta Encoding and Data Compression for HTTP (Corrected Version) (1997)

Caching in the World Wide Web currently follows a naive model, which assumes that resources are referenced many times between changes. The model also provides no way to update a cache entry if a resource does change, except by transferring the resource&apos;s entire new value. Several previous papers have proposed updating cache entries by transferring only the differences, or &quot;delta,&quot; between the cached entry and the current value. In this paper, we make use of dynamic traces of the full contents of HTTP messages to quantify the potential benefits of delta-encoded responses. We show that delta encoding can provide remarkable improvements in response size and response delay for an important subset of HTTP content types. We also show the added benefit of data compression, and that the combination of delta encoding and data compression yields the best results. We propose specific extensions to the HTTP protocol for delta encoding and data compression. These extensions are compatibl...
Local Optimization Approaches to Solve Dynamic Commercial Fleet Management Problems (2000)

Strategies are presented that a dispatcher may use to take advantage of real-time information to manage a fleet of trucks to provide truckload pickup-and-delivery service under time windows for service and computing time constraints. These strategies are hybrid in that they combine very efficient dynamic local heuristic rules for quick initial assignment, with formal optimization-based procedures for subsequent load reassignment decisions within the time requirements of the dynamic problem. Simulation experiments were performed to test these strategies under alternative specifications and parameter values. In the first stage, the acceptance decision and initial schedule of trucks were determined so as to respond rapidly to incoming customer requests for service. Then, some time is allowed to improve the schedule of trucks by swapping the assigned loads using local optimization at the reassignment stage. Two strategies are described to control the computation time of the reassignment optimization problem by controlling the problem size. The simulation results confirm the significant potential for reassignment strategies under real-time information to improve system performance and suggest that considerable improvement is attainable even under restricted problem formulations.
An initial experience in wearable monitoring sport systems (2010)

Until recent years most research involving the capture and analysis of biometric and/or physiological signals have been limited to a laboratory or otherwise controlled environment. Wearable technologies introduced a refinement to personal signal capturing by permitting a long-term on-person approach. Sensors, integrated circuits, textile integration and other elements are directly responsible for advancements in this area; however, in spite of the present progress there are still a number of obstacles to overcome for truly achieving seamless wearable monitoring technology (WMT). This article presents an overview of a generic monitoring systems architecture based on designs found in recent literature and commercially available solutions. A custom implementation based on commercially available components and evaluation boards is also presented, including some obtained data in varying body locations and/or activities.
Calreticulin Differentially Modulates Calcium Uptake and Release in the Endoplasmic Reticulum and Mitochondria (2002)

To study the role of calreticulin in Ca(2+) homeostasis and apoptosis, we generated cells inducible for full-length or truncated calreticulin and measured Ca(2+) signals within the cytosol, the endoplasmic reticulum (ER), and mitochondria with "cameleon" indicators. Induction of calreticulin increased the free Ca(2+) concentration within the ER lumen, [Ca(2+)](ER), from 306 +/- 31 to 595 +/- 53 microm, and doubled the rate of ER refilling. [Ca(2+)](ER) remained elevated in the presence of thapsigargin, an inhibitor of SERCA-type Ca(2+) ATPases. Under these conditions, store-operated Ca(2+) influx appeared inhibited but could be reactivated by decreasing [Ca(2+)](ER) with the low affinity Ca(2+) chelator N,N,N',N'-tetrakis(2-pyridylmethyl)ethylenediamine. In contrast, [Ca(2+)](ER) decreased much faster during stimulation with carbachol. The larger ER release was associated with a larger cytosolic Ca(2+) response and, surprisingly, with a shorter mitochondrial Ca(2+) response. The reduced mitochondrial signal was not associated with visible morphological alterations of mitochondria or with disruption of the contacts between mitochondria and the ER but correlated with a reduced mitochondrial membrane potential. Altered ER and mitochondrial Ca(2+) responses were also observed in cells expressing an N-truncated calreticulin but not in cells overexpressing calnexin, a P-domain containing chaperone, indicating that the effects were mediated by the unique C-domain of calreticulin. In conclusion, calreticulin overexpression increases Ca(2+) fluxes across the ER but decreases mitochondrial Ca(2+) and membrane potential. The increased Ca(2+) turnover between the two organelles might damage mitochondria, accounting for the increased susceptibility of cells expressing high levels of calreticulin to apoptotic stimuli.
High <i>T</i> <i>c</i> YBa2Cu3O7−<i>x</i> thin films on Si substrates by dc magnetron sputtering from a stoichiometric oxide target (1988)

Thin films of YBa2Cu3O7−x were deposited on Si substrates at 600–700 °C by dc magnetron sputtering from a stoichiometric oxide target. Resistivity measurement results indicate that these films are superconducting with a zero resistance Tc as high as 76 K, without further high-temperature post-annealing treatments. These films give both core and valence-band x-ray photoemission, and x-ray diffraction spectra similar to those for superconducting films prepared with a high-temperature post-annealing step. No significant diffusion of Si from the substrate into the film was detected for the films deposited at 650 °C or lower, according to depth profiles obtained using secondary ion mass spectrometry.
Surface-exposed conserved region of the streptococcal M protein induces antibodies cross-reactive with denatured forms of myosin (1993)

Vaccines based on a highly conserved cell surface exposed C-repeat region of the group A streptococcal M protein molecule have been found to induce protection against mucosal challenge by homologous and heterologous streptococcal serotypes. Rabbit hyperimmune antisera were produced to four partially overlapping peptides of the C-repeat region of M6 protein. These were examined by both direct and competitive ELISA and by Western blotting for their reactivity against mammalian coiled coil proteins such as laminin, myosin, light meromyosin, heavy meromyosin, and cardiac tropomyosin, and to the denatured forms of some of these molecules. All sera reacted strongly with the recombinant M6 protein molecule. In addition, antibodies to three of the peptides displayed generally low levels of cross-reactivity with at least one of the mammalian proteins, whereas antibodies to one peptide did not cross-react with any of the proteins tested. The observed reactivity was found to be directed predominantly against denatured forms of the mammalian molecules. For instance, the cleaved forms of myosin bound better to the cross-reactive antibodies than the intact molecule. Furthermore, heat-denatured heavy meromyosin competed severalfold better in competitive ELISA than the non-heat-denatured "native" form. Our results demonstrate that M protein peptides corresponding to epitopes shared among rheumatic fever-associated strains of streptococci can lead to the production of low levels of antibodies reactive with mammalian coiled coil molecules. These antibodies are directed against the denatured forms of these molecules.
The puzzling lateral flexible stalk of the ribosome (2003)

The lateral flexible stalk of the large ribosomal subunit is made of several interacting proteins anchored to a conserved region of the 28S (26S) rRNA termed the GTPase-associated domain or thiostrepton loop. This structure is demonstrated to adopt puzzling changes of conformation following the different steps of the elongation cycle. Some of these proteins termed the P-proteins in eukaryotes and L10 and L7/L12 in bacteria, present little structural similarities between Eubacteria on one side and Archae and Eukaryotes on the other side. However, up to now, these proteins seem to present a similar macromolecular organisation and they have been involved in the same functions. Convincing evidence attests that these proteins participate in elongation factor binding to the ribosome, and it has been suggested that these proteins might be evolved in a GTP hydrolysis activating protein activity. Involvement of these proteins in the translational mechanism is discussed. Moreover, in eukaryotes, small P-proteins are also found as isolated proteins in a cytoplasmic pool that exchanges with the ribosome-associated P-proteins. Moreover, a part of the ribosomal proteins is phosphorylated (hence their P-protein names). The biological signification of these particularities is discussed.