gebhart/patrepeval huggingface dataset:
Spliceosome-Mediated RNA <i>Trans</i> -Splicing with Recombinant Adeno-Associated Virus Partially Restores Cystic Fibrosis Transmembrane Conductance Regulator Function to Polarized Human Cystic Fibrosis Airway Epithelial Cells (2005)

We previously reported that spliceosome-mediated RNA trans-splicing (SMaRT), using recombinant adenoviral vectors expressing pre-trans-splicing molecules (PTMs), could partially restore cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel activity to polarized human DeltaF508 CF airway epithelia. Although these studies proved that SMaRT could correct CFTR mRNA defects, recombinant adenoviral infection from the basolateral surface was required because of inefficient infection from the apical membrane. Hence, applications of SMaRT technology for CF gene therapy require further testing with alternative, more clinically viable, vector systems. Furthermore, because recombinant adeno-associated virus (rAAV) vectors have packing limitations with respect to the size of the CFTR transgene insert, SMaRT correction of CFTR has the added attraction of a smaller transgene cassette. In the present study, we investigated whether rAAV vectors could effectively rescue CFTR chloride conductance in polarized human CF airway epithelial cells, using a SMaRT approach. AAV vectors were generated to carry a PTM engineered to bind intron 9 of CFTR pre-mRNA and then trans-splice the normal sequence for human CFTR exons 10-24 into the endogenous pre-mRNA. Human CF polarized airway epithelia were infected from the apical membrane with rAAV2 or rAAV5 CFTR-PTM vectors in the presence of proteasome-modulating agents (doxorubicin and N-acetyl-L-leucinyl-L-leucinyl-L-norleucinal) to enhance transduction. Epithelia were then evaluated for cAMP-sensitive short-circuit currents 2 weeks postinfection. Levels of CFTR correction seen with rAAV2 (1.07 +/- 0.24 microA) and rAAV5 (0.90 +/- 0.20 microA) CFTR-PTM vectors were similar, representing conductance equivalent to 14.2 and 13.6% of that observed in non-CF human polarized epithelia, respectively. RT-PCR analysis demonstrated the existence of wild-type CFTR transcript in CFTR-PTM-corrected epithelia, whereas only DeltaF508 mRNA was detected in polarized cells infected with control rAAV LacZ-PTM vectors. These results provide evidence that rAAV vectors are capable of using SMaRT to correct CFTR function after apical infection of human CF airway epithelia. The ability of CFTR-PTM-mediated correction to maintain endogenous CFTR regulation of the transgene product may further improve the efficacy of gene therapy for CF.
The physics of the near-field (2000)

Over the last decade, extensive exploitation of the different kinds of near-fields existing spontaneously or artificially in immediate proximity to the surface of materials has generated a considerable amount of new exciting developments. In this review the main physical properties of these peculiar fields are revisited. In a first stage, following a unified pedagogical model, we recall that the concept of near-field is not restricted to specific research areas, but actually covers numerous domains of contemporary physics (electronics, photonics, interatomic forces, phononics, ...). To a great extent, it will be shown that it mainly concerns phenomena involving evanescent fields (electronic density surface wave, evanescent light, local electrostatic and magnetic fields, ...) or localized interatomic or molecular interactions. In fact, the practical exploitation of these waves and local interactions was latent for a long time in physics until the beginning of the 1980s which was marked by the emergence and the success of local probe-based methods (STM, SFM, SNOM). Nowadays, various theoretical approaches and powerful numerical methods well suited to near-field physics are described in the literature. In the second part of this review, different original aspects of the near-field will be discussed with the intent of realizing control and optimization of its properties. In particular, the physics hidden inside the inverse decay length parameter η associated with all near-field concepts will be analysed in detail. This analysis may serve as a general framework for the design of physical or chemical compounds (photonic and electronic) able to control this fundamental parameter. We conclude the review by reconsidering an old and fundamental problem that can be summarized by the question, `What happens in the near-field interaction zone?'. Actually, this problem has been largely unaddressed in the near-field literature because what is needed in most practical situations is just the transmission coefficient of the whole device. However, when some dissipative elements interact with the near-field, this reasoning appears to be somewhat limited. In order to get more insight into this challenging question, we briefly give a state-of-the-art review of the relation between tunnelling events and energy dissipation inside the near-field.
Design and Preparation of 3,3‘-Disubstituted 2,2‘-Bis(oxazolyl)-1,1‘-binaphthyls (boxax):  New Chiral Bis(oxazoline) Ligands for Catalytic Asymmetric Wacker-Type Cyclization (1999)

New optically active 2,2'-bis(oxazolyl)-1,1'-binaphthyls (boxax) bearing various substituents (methoxycarbonyl, trimethylsilyl, (dimethylamino)carbonyl, formyl, and iodo) at their C3 and C3' positions were prepared via ortho-lithiation promoted by the oxazolyl groups. The 3,3'-disubstituted boxax ligands showed much higher enantioselectivity than the 3,3'-unsubstituted ones in the palladium(II)-catalyzed Wacker-type cyclization of an o-allylphenol. The cyclization of (E)-2-(2-methyl-2-butenyl)phenol (10) in the presence of a cationic palladium catalyst coordinated with (S)-2,2'-bis(4,4-dimethyloxazol-2-yl)-3,3'-bis(methoxycarbonyl)-1,1'-binaphthyl gave (S)-2-ethenyl-2-methyl-2,3-dihydrobenzofuran of 96% ee.
A comparison of RISC architectures (1989)

Three new RISC (reduced instruction set computer) architectures, the Intel i860, the Motorola 88000, and the Sun Microsystems Sparc, are compared. The relative importance of an architectural comparison, as opposed to a comparison of implementations, is discussed, followed by a high-level overview of each of the architectures. The relative strengths and weaknesses of the three architectures in a number of key areas are analyzed, and their overall relative strengths and weaknesses are summarized.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Low-cost coincidence-counting electronics for undergraduate quantum optics (2009)

Coincidence counting is a necessary ingredient for quantum optics experiments at the undergraduate level, but cost has created an entry barrier for many schools. We present a design of a coincidence-counting module that replaces the traditional method based on time-to-amplitude conversion and pulse-height analysis. Our module accepts inputs from up to four detectors, has a coincidence-time window of less than 10ns, and has a throughput of more than triple that of the traditional method. The cost of our coincidence-counting module is less than 5% of the cost of the traditional method.
Consensus Filters for Sensor Networks and Distributed Sensor Fusion (2006)

Consensus algorithms for networked dynamic systems provide scalable algorithms for sensor fusion in sensor networks. This paper introduces a distributed filter that allows the nodes of a sensor network to track the average of n sensor measurements using an average consensus based distributed filter called consensus filter. This consensus filter plays a crucial role in solving a data fusion problem that allows implementation of a scheme for distributed Kalman filtering in sensor networks. The analysis of the convergence, noise propagation reduction, and ability to track fast signals are provided for consensus filters. As a byproduct, a novel critical phenomenon is found that relates the size of a sensor network to its tracking and sensor fusion capabilities. We characterize this performance limitation as a tracking uncertainty principle. This answers a fundamental question regarding how large a sensor network must be for effective sensor fusion. Moreover, regular networks emerge as efficient topologies for distributed fusion of noisy information. Though, arbitrary overlay networks can be used. Simulation results are provided that demonstrate the effectiveness of consensus filters for distributed sensor fusion.
High power epitaxially-stacked varactor diode multipliers: performance and applications at W-band (2002)

Single-diode varactor multipliers capable of providing 260-mW CW (continuous wave) and 850-mW pulsed at 94 GHz are described. Improved performance for the generation of millimeter power at W-band is demonstrated using these single-diode doublers and cascaded multiplier chains for both CW and pulsed operation. Frequency multipliers using epitaxially stacked varactors have given an order-of-magnitude increase in power capability for varactor frequency multiplication; they now provide higher power per device chip than any other millimeter-wave solid-state device.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Characteristics of hybrid resonant converter operating on the utility line (2002)

A 1-/spl phi/ high-frequency transformer isolated AC-to-DC controlled rectifier using a hybrid parallel-series resonant converter bridge, operating on the utility line is described. Fixed frequency phase shift and variable frequency control schemes are used to obtain high line power factor and reduced line current harmonic distortion. Based on both approximate AC and state space analysis, design curves are obtained. A design example for 150 W converter is presented. The converter performance is evaluated with SPICE simulation (without active control) and by measurements on the 150 W breadboard model. The line power factor is maintained in the upper 90s with low harmonic distortion (/spl sime/16.5% at full load) even without active control. A simple active line current control technique has been used for wave-shaping the line current waveform close to sinusoid, and to reduce the line current total harmonic distortion.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Looking Beyond the Simple Scenarios: Combining Learners and Optimizers in 3D Temporal Tracking (2017)

3D object temporal trackers estimate the 3D rotation and 3D translation of a rigid object by propagating the transformation from one frame to the next. To confront this task, algorithms either learn the transformation between two consecutive frames or optimize an energy function to align the object to the scene. The motivation behind our approach stems from a consideration on the nature of learners and optimizers. Throughout the evaluation of different types of objects and working conditions, we observe their complementary nature - on one hand, learners are more robust when undergoing challenging scenarios, while optimizers are prone to tracking failures due to the entrapment at local minima; on the other, optimizers can converge to a better accuracy and minimize jitter. Therefore, we propose to bridge the gap between learners and optimizers to attain a robust and accurate RGB-D temporal tracker that runs at approximately 2 ms per frame using one CPU core. Our work is highly suitable for Augmented Reality (AR), Mixed Reality (MR) and Virtual Reality (VR) applications due to its robustness, accuracy, efficiency and low latency. Aiming at stepping beyond the simple scenarios used by current systems, often constrained by having a single object in the absence of clutter, averting to touch the object to prevent close-range partial occlusion or selecting brightly colored objects to easily segment them individually, we demonstrate the capacity to handle challenging cases under clutter, partial occlusion and varying lighting conditions.
Low resistance wavelength-reproducible <i>p</i>-type (Al,Ga)As distributed Bragg reflectors grown by molecular beam epitaxy (1993)

We report the reproducible molecular beam epitaxial growth of Be-doped piecewise linearly graded (Al,Ga)As distributed Bragg reflectors that have vertical series resistivities near bulk values. For mirrors with three linear segments per interface, the center wavelength reproducibility is 0.1% and the series resistivity is as low as 1.8×10−5 Ω cm2 for hole concentrations of 5×1018 cm−3. Measured reflectivities of 6.0% per interface are comparable to conventional single-linear-grade mirrors. Vertical-cavity surface-emitting lasers incorporating these mirrors exhibit record-low voltage thresholds of less than 1.5 V.
Characterization of murine T cell responses to peptides of the variable region of self T cell receptor <i>beta</i> -chains. (1993)

Immunization with peptides of the TCR may regulate cellular immune responses that are dominated by a particular TCR. However, no extensive study of the immunogenicity of peptides of different regions of the V beta chain of the TCR has been done. We have tested the immunogenicity of several V beta peptides in several strains of mice and characterized the cellular response to these peptides. We examined the ability of six strains of mice of H-2b, H-2d, or H-2k and of mouse lymphocyte stimulatory (Mls)-1a or Mls-1b haplotypes to respond to several peptides of the V beta 6 region and an NH2-terminal peptide of other V beta of the TCR. These include V beta 6 peptides 1-20, 32-48, 39-60, 48-75, 58-75, and V beta 3, V beta 8.1, and V beta 8.3 peptides 1-20. The various mouse strains respond to these peptides independently of deletion of V beta 6+ T cells from peripheral lymphocytes. All of the Mls deleting and nondeleting strains tested respond weakly to one peptide of V beta 6, V beta 6(39-60). Antibody titers were also demonstrated in BALB/c and DBA/2J to V beta 6(1-20), V beta 6(39-60) and V beta 6(48-75), but not to V beta 6(32-48). We demonstrated that T cells responding to V beta 6(32-48) produce IL-2 and IFN-gamma, consistent with the Th1 subset of T cells. None of the antipeptide antibodies recognized the intact V beta 6 TCR on the cell surface. In vitro antibody blocking studies with TCR peptides show that these peptides are presented by class II MHC to CD4+ T cells. We conclude that the T cell and B cell repertoires contain cells able to respond to peptides of self TCR and immunization with peptides induces CD4+ T cells and that these cells may have an immunoregulatory role.
Cellular and molecular composition of human skin in long‐term xenografts on SCID mice (1993)

We report on the immunophenotypical characterization of adult human skin transplanted onto severe combined immunodeficient (SCID) mice. Thirty animals were followed for up to 12 months after receiving split-thickness xenografts, of which 28 were tolerated for the whole test period. Antigen mapping revealed an almost complete preservation of human cellular and extracellular tissue components in long-term transplants including skin immune cells (Langerhans-cells, macrophages, lymphocytes) and also parts of the engrafted endothelium. Hence, xenografts on SCID mice offer a versatile experimental tool for the in vivo study of both human skin immune cell function and endothelial cell-mediated interactions in an environment completely devoid of interferences by adoptive host immune response.
Nitrogen heterocycle-containing materials for highly efficient phosphorescent OLEDs with low operating voltage (2014)

Since their discovery, phosphorescent organic light-emitting diodes (PHOLEDs) have been developed for more than ten years. To improve the electricity-to-light conversion efficiency of PHOLEDs, it is of great importance to improve their external quantum efficiency and to decrease their operating voltage. The improvement of functional materials such as host materials and carrier transport materials, especially electron transport materials, plays the most important role in reaching these targets. In this feature article, we provide a brief review of the recent developments in nitrogen heterocycle-containing functional materials for PHOLEDs. Special attention is paid to the issue of how to utilise the molecular design strategy in achieving the development of high-performance host and electron transport materials and of how to use these nitrogen heterocycle-containing materials to create PHOLEDs with efficiencies and operating voltages that reach the theoretical limits.
Two-photon photoacoustics ultrasound measurement by a loss modulation technique (2013)

In this work, we investigated the principle of the two-photon absorption (TPA) detection with a loss modulation technique, and first demonstrated the existence of two-photon photoacoustics ultrasound excited by a femtosecond high repetition rate laser. By using the AO modulation with different modulation frequencies, we successfully create the beating of the light signal when the two arms of the beams are both spatial and temporal overlapping. The pulse train of the femtosecond laser causes the narrow band excitation, providing the frequency selectivity and sensitivity. Moreover, the pulse energy is no more than 15nJ/pulse, which is at least 3 orders of magnitude smaller than that of the nanosecond laser, and therefore prevents the thermal damage of the sample. With the help of lock-in detection and a low noise amplifier, we can separate the signal of two-photon absorption from one-photon absorption. We used an ultrasonic transducer to detect the response of the sample, and verified the existence of the two-photon photoacoustics ultrasound generating by the femtosecond laser. Several contrast agents, such as the black carbon solution, the fluorescence dye and the nano-particles, were used in the experiment. In the end, we demonstrated the application, two photo-acoustic imaging, which provides the high spatial resolution (<10μm) and large penetration depth (~1mm), to the simulated biological tissue. This is a milestone to develop the two-photon photoacoustics microscopy, which, in principle, has the great potential to achieve the in vitro and in vivo high resolution deep tissue imaging.
Theoretische Grundlagen der ökonomischen Umweltbewertung (2001)

Bevor wir in Kapitel 3 auf das Konzept des Nichtgebrauchswerts eingehen, beschäftigt sich dieses Kapitel zunächst mit den allgemeinen Grundlagen der ökonomischen Umweltbewertung. An die nun folgende Betrachtung der normativen und theoretischen Basis der neoklassischen Wohlfahrtstheorie schließt sich hierin in den Abschnitten 2.2 und 2.3 die Darstellung derjenigen Konzepte der Haushaltstheorie bzw. der Theorie der individuellen Wohlfahrtsmessung an, auf denen die Ausführungen im weiteren Verlauf der Arbeit, insbesondere in den Kapiteln 3.2 und 8, basieren.
Indicators of Child Well-Being: the Promise for Positive Youth Development (2004)

In the current U.S. indicators system, measures of child well-being focus primarily on negative outcomes and problems. We measure and track those behaviors that adults wish to prevent. For the most part, the indicators system does not monitor positive development and outcomes. Such a system of child well-being indicators lacks the breadth and balance required in a science-based measurement system. Moreover, it lacks measures of the kinds of constructs that resonate among adolescents themselves and adults. Measures are needed for multiple domains of development, including educational achievement and cognitive attainment, health and safety, social and emotional development, and self-sufficiency. Positive outcomes are often critiqued as soft, highlighting the importance of rigorous conceptualization and measurement, including conceptual clarity and face validity, age appropriate measures, and psychometric rigor. In addition, constructs and measures need to be presented in ways that are understandable to policy makers and the public and that work across varied subgroups and levels of governance. Ideally, comparable measures will be used for indicators, for program evaluation, and in basic research studies of child and adolescent development.
Distribution and fine structural analysis of undifferentiated cells in the primate subependymal layer. (1991)

The subependymal layer (SEL) of the postnatal marmoset, a simian primate, has been investigated by histological and electron microscopic techniques. Although well documented in rodents, little is known about this layer in primates. The distribution of the SEL in marmosets is most extensive at birth around the anterior lateral ventricles, where the layer is generally 5-10 cells deep; however, there is considerable regional variation. With age the size of the SEL decreases dramatically, becoming very poorly demarcated in adult animals. Ultrastructurally, many subependymal cells in neonatal and young brains display the features of undifferentiated cells, although neurons and glia are also present. Cells displaying features intermediate between astrocytes and undifferentiated SEL cells are occasionally encountered. In adults undifferentiated cells are seen rarely and the former SEL is composed primarily of glial and neuronal processes. Thus the layer in primates probably represents a site of continued cellular differentiation in the postnatal brain and as such must play an important role in the final stages of cortical development.
A skin-integrated transparent and stretchable strain sensor with interactive color-changing electrochromic displays (2017)

In this study, we report on the development of a stretchable, transparent, and skin-attachable strain sensor integrated with a flexible electrochromic device as a human skin-inspired interactive color-changing system. The strain sensor consists of a spin-coated conductive nanocomposite film of poly(vinyl alcohol)/multi-walled carbon nanotube/poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) on a polydimethylsiloxane substrate. The sensor exhibits excellent performance of high sensitivity, high durability, fast response, and high transparency. An electrochromic device (ECD) made of electrochemically synthesized polyaniline nanofibers and V<sub>2</sub>O<sub>5</sub> on an indium-tin-oxide-coated polyethylene terephthalate film experiences a change in color from yellow to dark blue on application of voltage. The strain sensor and ECD are integrated on skin via an Arduino circuit for an interactive color change with the variation of the applied strain, which enables a real-time visual display of body motion. This integrated system demonstrates high potential for use in interactive wearable devices, military applications, and smart robots.
SVMs Modeling for Highly Imbalanced Classification (2008)

Traditional classification algorithms can be limited in their performance on highly unbalanced data sets. A popular stream of work for countering the problem of class imbalance has been the application of a sundry of sampling strategies. In this paper, we focus on designing modifications to support vector machines (SVMs) to appropriately tackle the problem of class imbalance. We incorporate different "rebalance" heuristics in SVM modeling, including cost-sensitive learning, and over- and undersampling. These SVM-based strategies are compared with various state-of-the-art approaches on a variety of data sets by using various metrics, including G-mean, area under the receiver operating characteristic curve, F-measure, and area under the precision/recall curve. We show that we are able to surpass or match the previously known best algorithms on each data set. In particular, of the four SVM variations considered in this paper, the novel granular SVMs-repetitive undersampling algorithm (GSVM-RU) is the best in terms of both effectiveness and efficiency. GSVM-RU is effective, as it can minimize the negative effect of information loss while maximizing the positive effect of data cleaning in the undersampling process. GSVM-RU is efficient by extracting much less support vectors and, hence, greatly speeding up SVM prediction.