gebhart/patrepeval huggingface dataset:
Signal transduction of phagocytosis (1995)

The interaction of particles with certain cell surface receptors initiates intracellular signalling pathways that ultimately lead to submembranous actin filament assembly, pseudopod extension, and the ingestion of the particles. Here, Steven Greenberg reviews recent evidence implicating various signalling events in phagocytosis--in particular, activation of tyrosine kinases and phosphatidylinositol 3-kinase--and speculates how they might regulate the actin cytoskeleton.
Insertion of a Multibasic Cleavage Motif into the Hemagglutinin of a Low-Pathogenic Avian Influenza H6N1 Virus Induces a Highly Pathogenic Phenotype (2010)

The highly pathogenic avian influenza (HPAI) virus phenotype is restricted to influenza A viruses of the H5 and H7 hemagglutinin (HA) subtypes. To obtain more information on the apparent subtype-specific nature of the HPAI virus phenotype, a low-pathogenic avian influenza (LPAI) H6N1 virus was generated, containing an HPAI H5 RRRKKR [downward arrow] G multibasic cleavage site (MBCS) motif in HA (the downward arrow indicates the site of cleavage). This insertion converted the LPAI virus phenotype into an HPAI virus phenotype in vitro and in vivo. The H6N1 virus with an MBCS displayed in vitro characteristics similar to those of HPAI H5 viruses, such as cleavage of HA(0) (the HA protein of influenza A virus initially synthesized as a single polypeptide precursor) and virus replication in the absence of exogenous trypsin. Studies of chickens confirmed the HPAI phenotype of the H6N1 virus with an MBCS, with an intravenous pathogenicity index of 1.4 and systemic virus replication upon intranasal inoculation, the hallmarks of HPAI viruses. This study provides evidence that the subtype-specific nature of the emergence of HPAI viruses is not at the molecular, structural, or functional level, since the introduction of an MBCS resulted in a fully functional virus with an HPAI virus genotype and phenotype.
Structural and optical properties of strontium titanate thin films prepared by spray pyrolysis (2014)

Strontium titanate (SrTiO3) thin films sample were prepared by using spray pyrolysis technique onto a glass substrate. The thin films were deposited using various concentrations of 0.2 – 1.0 M strontium nitrate (Sr(NO3)2) and titanium tetraisopropoxide (TTIP) at constant temperature 400oC and a flow rate of 3 mL/min oxygen carrier gas. The films were annealed at temperature range of 400oC to 650oC. The films were characterized using UV-Visible spectrophotometer, X-ray diffractometer (XRD), atomic force microscope (AFM), Fourier transform infra-red spectrometer (FTIR) and photoluminescence (PL) spectrometer. The samples showed a high transmittance of more than 75% in the visible region, with band gap energy in the range of 3.4 eV to 4.0 eV. The band gap energy of the films decreased from 3.9 eV to 3.5 eV as the annealing temperature increased from 400oC to 500oC. The films deposited at 400oC showed an amorphous phase. At an annealing temperature of 450oC, the thin films sample turned into polycrystalline phase. The intensity of (110) orientation was the dominant phase of strontium titanate at annealing temperature of 650oC. FTIR measurements showed that symmetrical OH absorption band exists in all the films and Ti-O bonding only can be observed at an annealing temperature 500oC above. The grain sizes and surface roughness of the films increased with increasing annealing temperatures. In this study, the strontium titanate (SrTiO3) thin films did not show luminescent characteristic.
Catalytic Antibodies Induced by a Zwitterionic Hapten (2001)

A zwitterionic hapten 4 featuring both positively and negatively charged functional groups was designed and synthesized with the goal of generating catalytic antibodies for the hydrolysis of ester 6 and amide 7. Of the 36 monoclonal antibodies specific to BSA-4 (bovine serum albumin) that were isolated, six accelerated the hydrolysis of 6. Two catalytic antibodies with distinctively different and representative kinetic behaviors were selected for detailed kinetic studies. Whereas H8-2-6F11 showed burst kinetic behavior, which can be attributed to the formation of an acyl intermediate, H8-1-2D5 did not, but it did exhibit high multiple turnover activity. The rate of hydrolysis of 6 catalyzed by H8-1-2D5 followed Michaelis-Menten kinetics; the apparent values of the Michaelis-Menten constant Km and the catalytic constant kcat were 488 microM and 3.5 min(-1), respectively. The catalytic rate enhancement (kcat/kun) observed for H8-1-2D5 was 1.3 x 10(5), which is approximately two orders of magnitude greater than those for monofunctional haptens. Thus H8-1-2D5 compares well in catalytic activity with antibodies isolated by a related approach called heterologous immunization.
A Genetic System for Rhesus Monkey Rhadinovirus: Use of Recombinant Virus To Quantitate Antibody-Mediated Neutralization (2006)

Rhesus monkey rhadinovirus (RRV), a simian gamma-2 herpesvirus closely related to the Kaposi sarcoma-associated herpesvirus, replicates lytically in cultured rhesus monkey fibroblasts and establishes persistence in B cells. Overlapping cosmid clones were generated that encompass the entire 130-kilobase-pair genome of RRV strain 26-95, including the terminal repeat regions required for its replication. Cloned RRV that was produced by cotransfection of overlapping cosmids spanning the entire RRV26-95 genome replicated with growth kinetics and to titers similar to those of the parental, uncloned, wild-type RRV26-95. Expression cassettes for secreted-engineered alkaline phosphatase (SEAP) and green fluorescent protein (GFP) were inserted upstream of the R1 gene, and the cosmid-based system for RRV genome reconstitution was used to generate replication-competent, recombinant RRV that expressed either the SEAP or GFP reporter gene. Using the SEAP and GFP recombinant RRVs, assays were developed to monitor RRV infection, neutralization, and replication. Heat-inactivated sera from rhesus monkeys that were naturally or experimentally infected with RRV were assayed for their ability to neutralize RRV-SEAP and RRV-GFP infectivity using rhesus monkey fibroblasts. Sera from RRV-positive monkeys, but not RRV-negative monkeys, were consistently able to neutralize RRV infectivity when assayed by the production of SEAP activity or by the ability to express GFP. The neutralizing activity was present in the immunoglobulin fraction. Of the 17 rhesus monkeys tested, sera from rhesus monkey 26-95, i.e., the monkey that yielded the RRV 26-95 isolate, had the highest titer of neutralizing activity against RRV26-95. This cosmid-based genetic system and the reporter virus neutralization assay will facilitate study of the contribution of individual RRV glycoproteins to entry into different cell types, particularly fibroblasts and B cells.
Tales of the Tail (2014)

Interactive services often have large-scale parallel implementations. To deliver fast responses, the median and tail latencies of a service's components must be low. In this paper, we explore the hardware, OS, and application-level sources of poor tail latency in high throughput servers executing on multi-core machines.
Flexible energy storage devices based on graphene paper (2011)

Recently, great interest has been aroused in flexible/bendable electronic equipment such as rollup displays and wearable devices. As flexible energy conversion and energy storage units with high energy and power density represent indispensable components of flexible electronics, they should be carefully considered. However, it is a great challenge to fabricate flexible/bendable power sources. This is mainly due to the lack of reliable materials that combine both electronically superior conductivity and mechanical flexibility, which also possess high stability in electrochemical environments. In this work, we report a new approach to flexible energy devices. We suggest the use of a flexible electrode based on free-standing graphene paper, to be applied in lithium rechargeable batteries. This is the first report in which graphene paper is adopted as a key element applied in a flexible lithium rechargeable battery. Moreover graphene paper is a functional material, which does not only act as a conducting agent, but also as a current collector. The unique combination of its outstanding properties such as high mechanical strength, large surface area, and superior electrical conductivity make graphene paper, a promising base material for flexible energy storage devices. In essence, we discover that the graphene based flexible electrode exhibits significantly improved performances in electrochemical properties, such as in energy density and power density. Moreover graphene paper has better life cycle compared to non-flexible conventional electrode architecture. Accordingly, we believe that our findings will contribute to the full realization of flexible lithium rechargeable batteries used in bendable electronic equipments.
Relaxation dynamics of ionic liquid—VO2 interfaces and influence in electric double-layer transistors (2012)

Oxide semiconductor systems are often electrostatically doped with ionic liquids as gate insulators towards modulating carrier density and inducing phase transitions, while simultaneously serving as a means to probe their electronic phase diagram. The electronic and electrochemical properties of ionic liquid/correlated oxide interfaces are, therefore, important in interpreting such field-effect phenomena. Here, we use DEME-TFSI and VO2 as a model system to investigate the interface properties, slow relaxations, as well as field-effect in electric double layer transistor geometry. The stability of these interfaces is probed by combination of current-voltage measurements, x-ray photoelectron spectroscopy, impedance spectroscopy, and constant current charging. Three-terminal field effect transistor-type devices fabricated by photolithography are used to investigate kinetics of channel resistance modulations under varying gate bias polarity. Bias regions for reversible modulation of channel conductance have been determined. A time-dependent transconductance effect and as large as 20× increase in conductance are observed.
Data lake management (2019)

The ubiquity of data lakes has created fascinating new challenges for data management research. In this tutorial, we review the state-of-the-art in data management for data lakes. We consider how data lakes are introducing new problems including dataset discovery and how they are changing the requirements for classic problems including data extraction, data cleaning, data integration, data versioning, and metadata management.
Differential responses to challenge with live and dead Mycobacterium bovis Bacillus Calmette-Guérin (1997)

Bacillus Calmette-Guérin (BCG) vaccination has been shown to protect against challenge with virulent Mycobacterium tuberculosis in a range of experimental animal models: in each case, protective efficacy requires vaccination with live bacteria. With the goal of moving to a new generation of safer, nonliving vaccines, efforts have been made to identify the factors that determine the efficacy of live vaccination. We show that injection of live, but not dead, BCG induces localized swelling in the mouse footpad model. Live and dead bacteria induce similar responses during the first week after vaccination as determined by immunohistochemical analysis of the site of injection and of the draining lymph node. The subsequent differential response is characterized by migration of acid-fast bacilli to the draining lymph node in the case of the live vaccine. This is accompanied by an increase in mononuclear cells in the lymph node and by expression of inducible nitric oxide synthase (iNOS) both in the lymph node and at the site of injection. The ability of the bacteria to migrate to the lymph node may be an important element in the efficacy of live BCG vaccination.
Multiple Cytokines Are Required to Induce Hepatocyte Nitric Oxide Production and Inhibit Total Protein Synthesis (1990)

The etiology and mechanisms by which severe trauma or sepsis induce hepatic failure are unknown. Previously we showed that Kupffer cells (KC), the fixed macrophages of the liver, induce a profound decrease in hepatocyte (HC) total-protein synthesis when exposed to endotoxin. Furthermore we demonstrated that endotoxin-activated KCs induce these changes in HC protein synthesis through the induction of a novel L-arginine-dependent biochemical pathway within the HC. In this pathway, the guanido nitrogen of L-arginine is converted to the highly reactive molecule nitric oxide (NO.). To identify the KC factors that act as signals for induction of HC NO. biosynthesis, recombinant cytokines were added to HC cultures and HC nitrogen oxide production and protein synthesis levels were determined. We found that no single cytokine, but rather a specific combination of tumor necrosis factor, interleukin-1, interferon-gamma, and endotoxin, were required for maximal induction of HC nitrogen oxide production. This specific combination of cytokines induced a 248.8 +/- 26.0 mumol/L (micromolar) increase in HC nitrogen oxide production and simultaneously inhibited HC total protein synthesis by 36.1% +/- 3.1%. These data demonstrate that multiple cytokines, produced by endotoxin-activated KC, induce the production of NO. within HC, which in turn leads to the inhibition of HC total-protein synthesis.
Deep considerations on LED package technology (2015)

This paper focus our attention on the deep considerations for LED package technology. The research includes the following content: (i) the influence of binding force between the chip and substrate, the leakage rate of silver paste conductive adhesive, the failure rate of light emission based on different glue quantities from different companies are studied carefully; (ii) a variety of abnormalities appeared in the process of die bond are demonstrated, and some failure reasons of lighting extracting are analyzed, and the correct processing method for dealing of these abnormalities are suggested; (iii) the influence of the size, abrasiveness, the tear force, and the power of porcelain mouth on package product performance and the quality are discussed; (iv) the influence of phosphor particle size, the amount of anti-settling powder, the viscosity of the fluorescent glue, and the baking conditions on white LED color coordinates and color concentration are analyzed. For same batch of the same chip packaging technology with same device setting parameters, there are still existing a certain bias in lighting output power, color coordinates and color concentration etc. It is found that many key factors are coming from the less consideration for package materials. The uneven distribution of mixing or dispensing phosphors is also a key factor to influence the package quality of SMD and COB light source.
Research on the difference between rectal temperature and axillary temperature in febrile children (2009)

Objective To explore the difference in rectal temperature and axillary temperature adding 0.6℃. Method A total of 101 children were measured rectal temperature and axillary temperature at the same time. Results The difference between the rectal temperature and the axillary temperature varied from 0℃to 1.7℃. There was significant difference between the rectal temperature and the axillary temperature adding 0.6℃(P0. 05). Conclude Rectal tem- perature should be taken in the febrile children.
An optimal auditory filter (2002)

The optimality of the peripheral auditory filter is investigated using operator methods applied to a scale representation. A 'gammachirp' function, which consists of a frequency modulated carrier and an envelope of a gamma distribution function, is found to be the optimal auditory filter in terms of minimal uncertainty if the time-scale representation is calculated in the auditory system. The gammatone function is the first-order approximation of the gammachirp function. The gammachirp function has an asymmetrical Fourier power spectrum and fits the psychoacoustic auditory filter shape better than the gammatone function. Wavelet configuration is optimal for the auditory filterbank in terms of invariability in the scale representation. Data for equivalent rectangular bandwidth (ERB) suggest the wavelet gammachirp filterbank is feasible for characteristic frequencies above 800 Hz.
Taurine-supplemented total parenteral nutrition and taurine status of malnourished cancer patients. (1994)

The status of plasma taurine and whether its concentration can be influenced by total parenteral nutrition (TPN) was determined in 51 malnourished fasting cancer patients after surgery and 7-14 days after starting TPN providing 41 +/- 2 kcal, 0.30 +/- 0.02 g N kg-1.day-1 and 40 mg pyridoxine. Plasma taurine was 50% lower in patients than in control subjects. Plasma taurine was significantly greater than baseline only after 14 days of TPN. We also studied the effects of surgery and taurine supplementation (8.6 mg.kg-1.day-1) on plasma and urine taurine concentrations in 12 malnourished patients. Preoperatively, all patients had normal plasma taurine concentrations; postoperatively, it was in the deficient range in 4 patients. Taurine-supplemented patients initially had higher than baseline concentrations; by day 10, none had subnormal levels. Subnormal taurine concentrations commonly occur in malnourished postoperative cancer patients; surgery further precipitates their fall. Plasma concentrations were maintained only with taurine-supplemented TPN.
Periodontal ligament and gingival fibroblasts from periodontitis patients are more active in interaction with Porphyromonas gingivalis (2011)

Periodontal ligament and gingival fibroblasts respond to P. gingivalis in a different manner and may play different roles in periodontitis. Both subsets of fibroblasts from patients appear more active in interaction with P. gingivalis. Moreover, periodontal ligament fibroblasts from P. gingivalis-positive donors are more responsive to an in vitro P. gingivalis challenge.
Silicon Photonic Gas Sensing (2019)

We present a photonic chip sensor platform for near-infrared laser absorption spectroscopy, which incorporates an uncooled III-V laser and detector, on-chip methane reference cell, and 30cm-long evanescent field waveguides, integrated on a silicon photonics substrate.
A <i>Drosophila</i> Model To Identify Polyamine−Drug Conjugates That Target the Polyamine Transporter in an Intact Epithelium (2007)

Polyamine transport is elevated in many tumor types, suggesting that toxic polyamine-drug conjugates could be targeted to cancer cells via the polyamine transporter (PAT). We have previously reported the use of Chinese hamster ovary (CHO) cells and its PAT-deficient mutant cell line, CHO-MG, to screen anthracene-polyamine conjugates for their PAT-selective targeting ability. We report here a novel Drosophila-based model for screening anthracene-polyamine conjugates in a developing and intact epithelium ( Drosophila imaginal discs), wherein cell-cell adhesion properties are maintained. Data from the Drosophila assay are consistent with previous results in CHO cells, indicating that the Drosophila epithelium has a PAT with vertebrate-like characteristics. This assay will be of use to medicinal chemists interested in screening drugs that use PAT for cellular entry, and it offers the possibility of genetic dissection of the polyamine transport process, including identification of a Drosophila PAT.
Fibrosis in undifferentiated (anaplastic) thyroid carcinomas: evidence for a dual action of tumour cells in collagen type I synthesis (2000)

The mechanisms involved in stromal reactions and fibrosis in solid malignant tumours are incompletely understood. In the present study, collagen type I production was investigated in tissues and cell lines derived from human undifferentiated (anaplastic) thyroid carcinomas, a highly aggressive, often fibrotic malignancy with mesenchymal phenotype. In situ hybridization showed the expression of pro-alpha1(I) collagen mRNA throughout the stromal part of the tumours. However, immunofluorescence staining using an anti-pro-collagen type I antibody revealed the synthesis of pro-collagen type I protein mainly in stromal cells juxtaposed to nests of tumour cells. In one out of five tissue samples from human undifferentiated thyroid carcinomas, pro-alpha1(I) collagen mRNA expression was also found in a small number of tumour cells. Several well-characterized cell lines established from undifferentiated thyroid carcinomas, two from tumours included in the present study, expressed both pro-alpha1(I) collagen and prolyl 4-hydroxylase mRNA, and three of these cell lines also synthesized native triple-helical collagen type I. Taken together, these data suggest that stromal fibroblasts are the main producers of collagen type I in anaplastic thyroid tumours. The carcinoma cells seem to play a regulatory role, stimulating the synthesis of collagen type I protein in the surrounding stroma by increasing pro-alpha1(I) collagen mRNA translation. However, collagen type I production by the carcinoma cells might also contribute to the marked desmoplasia commonly seen in these tumours.
The effect of propofol and sevoflurane on antioxidants and proinflammatory cytokines in a porcine ischemia–reperfusion model (2015)

We concluded that propofol, compared with sevoflurane, can significantly inhibit ROS formation on a cell level. In addition, propofol can significantly inhibit TNF-α formation of monocytes and coronary smooth muscle cells but not aortic smooth muscle cells.
A comparative study of topological properties of hypercubes and star graphs (1994)

Undertakes a comparative study of two important interconnection network topologies: the star graph and the hypercube, from the graph theory point of view. Topological properties are derived for the star graph and are compared with the corresponding properties of the hypercube. Among other results, the authors determine necessary and sufficient conditions for shortest path routing and characterize maximum-sized families of parallel paths between any two nodes of the star graph. These parallel paths are proven of minimum length within a small additive constant. They also define greedy and asymptotically balanced spanning trees to support broadcasting and personalized communication on the star graph. These results confirm the already claimed topological superiority of the star graph over the hypercube.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
IL-23–Independent Induction of IL-17 from γδT Cells and Innate Lymphoid Cells Promotes Experimental Intraocular Neovascularization (2013)

Choroidal neovascularization (CNV) is a characteristic of age-related macular degeneration. Genome-wide association studies have provided evidence that the immune system is involved in the pathogenesis of age-related macular degeneration; however, the role of inflammatory cytokines in CNV has not been established. In this study, we demonstrated that IL-17 had a strong potential for promoting neovascularization in a vascular endothelial growth factor-independent manner in laser-induced experimental CNV in mice. Infiltrated γδT cells and Thy-1(+) innate lymphoid cells, but not Th17 cells, were the main sources of IL-17 in injured eyes. IL-23 was dispensable for IL-17 induction in the eye. Instead, we found that IL-1β and high-mobility group box 1 strongly promoted IL-17 expression by γδT cells. Suppression of IL-1β and high-mobility group box 1, as well as depletion of γδT cells, reduced IL-17 levels and ameliorated experimental CNV. Our findings suggest the existence of a novel inflammatory cytokine network that promotes neovascularization in the eye.