gebhart/patrepeval huggingface dataset:
Expression of protective and cardiac tissue cross-reactive epitopes of type 5 streptococcal M protein in Escherichia coli (1985)

The immunochemical properties of type 5 M protein antigens that were expressed in Escherichia coli K-12 by recombinant lambda bacteriophages isolated from a gene bank of serotype 5 Streptococcus pyogenes have been analyzed in detail. M proteins from partially purified bacteriophage lysates displayed precipitin lines of identity with a purified peptic extract of type 5 M protein (pep M5) in immunodiffusion assays. Immunoblot analyses of the M protein-positive lysates demonstrated that the cloned M protein component resided in five polypeptides with relative molecular weights of 57,900 (57.9K), 55.4K, 52.9K, 40.0K, and 32.6K. The hybrid lambda phage (lambda M5)-produced M protein contained immunoprotective epitopes; lambda M5 protein inhibited opsonization of type 5 streptococci by pep M5 antibodies, and antiserum raised against lambda M5 lysates opsonized type 5 streptococci. Each of the five antigenic polypeptides of the recombinant phage M protein also shared epitopes with human heart tissue, as demonstrated by the reactivity of immunoblots of lambda M5 antigens separated on sodium dodecyl sulfate gels with anti-pep M5 antibodies absorbed to and eluted from human heart sarcolemmal membranes. Moreover, antiserum raised against the lambda M5 lysates reacted with sarcolemmal membrane proteins with relative molecular weights of 200K, 59K, 55K, 53K, and 27K as determined by immunoblot analyses. These results demonstrate that the structural gene coding for type 5 streptococcal M protein which was inserted into lambda DNA expresses immunoprotective epitopes, some of which are shared with human heart tissue.
Permeabilized Streptococcus thermophilus in the preparation of low-lactose milk (1995)

Several compounds with detergent activity were used to permeabilize Streptococcus thermophilus and allow a high level expression of intracellular beta-galactosidase. Treatment with SDS, Triton X-100, sodium deoxycholate and oxgall suppressed cell growth for several hours, which permitted the use of permeabilized S. thermophilus biomass in milk without concomitant production of lactic acid. The addition of cell preparations directly or entrapped in an agarose-gel support resulted in up to 90% or higher reduction of lactose content in milk.
Effect of the Catalyst Bed Configuration on the Partial Oxidation of Liquid Hydrocarbons (2013)

Rh-substituted pyrochlores have been shown to be excellent diesel reforming catalysts. However, it is desirable to reduce the amount of this expensive material while maintaining an acceptable level of hydrogen production. This study demonstrates that a segmented catalyst bed approach can be used to achieve this objective. Two strategies were examined: (1) promotion of the indirect reforming mechanism with a combustion catalyst in the reactor inlet, followed by a reforming catalyst, and (2) placement of catalysts in regions of the reactor that have conditions in which they are less likely to deactivate. The first approach demonstrated that a Ni-substituted barium hexaaluminate catalyst can be used in the reactor inlet to promote combustion with a Rh-substituted pyrochlore in the reactor outlet, but the combustion catalyst should fill less than 50% of the reactor. The second approach showed a benefit in the use of a sulfur-tolerant noble metal catalyst in the reactor outlet and that a significant portion of the carbon formed on the Ni-substituted pyrochlore is located in the last 25% of the catalyst bed.
Utilization of sulfur and crumb rubber in asphalt modification (2013)

Abstract In this study, waste crumb rubber and sulfur were utilized to enhance the performance of asphalt binder for pavement applications. About 20–50% of sulfur and 1–6% crumb rubber were used. Melt properties were investigated using thermal analysis, dynamic and steady shear rheology, and artificial aging. Rheological tests were carried out in ARES rheometer. Both steady and dynamic shear rheology showed that crumb rubber improved the viscoelastic properties of the sulfur‐extended asphalt binder. Crumb rubber modification reduced temperature susceptibility of sulfur/asphalt, and increased the upper grading (performance) temperature of sulfur asphalt. The combined effect of sulfur and crumb rubber reduced the activation energy compared with that of pure asphalt. Zero‐shear viscosity and strategic highway research program rutting parameter ( G*/ sinδ) improved by crumb rubber incorporation into the sulfur asphalt binder. Short‐term aging improved G*/ sinδ with slight increase in activation energy. The addition of sulfur to asphalt matrix increased the viscoelastic properties ( G ′ and G ″) of sulfur asphalt. The addition of crumb rubber to sulfur asphalt enhanced the temperature resistance of the binder. Utilization of waste crumb rubber and sulfur in asphalt modification proved to enhance asphalt pavement life. In addition, utilization of such wastes can help in meeting the extra demand for asphalt, reduce the pavement cost, and help in solving a waste disposal problem. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014 , 131 , 40046.
A comparative study of wideband and dual-band digital predistortion architectures (2015)

In this paper, the wideband and dual-band architectures for digital predistorters (DPD) are compared in terms of the DPD models used, hardware implementations, complexity and linearization performance. The advantages and disadvantages of each architecture are outlined and hardware measurements are performed using an amplifier prototype to illustrate the differences between the two approaches.
Modification of human leukocyte interferon pharmacology with a monoclonal antibody. (1985)

The antitumor and antiviral properties of the interferons have been well established. However, the usefulness of the interferons may be limited, in part, because of rapid clearance from the plasma and degradation by plasma or tissue enzymes. A monoclonal antibody (IFG-252.2) was developed which binds to recombinant DNA-produced human alpha-interferon (rIFN-alpha A) without measurably reducing its in vitro antiviral or antiproliferative properties. Pharmacokinetic studies of rIFN-alpha A:antibody complex in the intact, anesthetized rat showed that rIFN-alpha A activity cleared from plasma 3-fold slower than found after injection of free rIFN-alpha A. This resulted in a 15-fold increase in its calculated area under concentration curve compared to that of free rIFN-alpha A. These studies suggest that interferon bound to a monoclonal antibody may provide a means to prevent the normal clearance and degradation of free interferon and may result in prolonged antitumor and antiviral plasma activity in vivo. Furthermore, it suggests that monoclonal antibodies to various biologically active agents may be used to favorably alter their pharmacokinetics while leaving their biological activity unaltered.
Carbon nanotube catalysis by metal silicide: resolving inhibition versus growth (2006)

The catalytic activity of metal silicide for carbon nanotube (CNT) nucleation and growth is reported by systematically evaluating CNT synthesis on different metal silicide phases compared with pure metal catalysts. Using a combination of high-resolution microscopy, x-ray diffraction (XRD) and x-ray photoelectron spectroscopy (XPS), the influence of various pretreatment annealings on 1, 5 and 10 nm Ni and Co films sputter deposited on pristine Si(100) and SiO2 substrates has been explored in relation to their ability to catalyse CNT growth under a broad range of synthesis conditions. Under certain pretreatment and growth conditions metal silicide nanoparticles are as catalytically active as pure metal nanoparticles. The impact of these findings is significant. The considerable expertise on metal silicide already existing in silicon technology can be applied and extended for the integration of CNTs in electronics.
Compact colorless tunable dispersion compensator with 1000-ps/nm tuning range for 40-gb/s data rates (2006)

A novel tunable dispersion compensator (TDC) with /spl plusmn/500-ps/nm tuning range and bandwidth support for 40-Gb/s signals is described. The TDC is constructed from a waveguide grating router (WGR) that provides very high spatial dispersion and a deformable cylindrical mirror for applying quadratic spatial phase across the dispersed wavefront. The WGR's 100-GHz free-spectral range (FSR) allows the device to simultaneously apply the same dispersion to all wavelength-division multiplexing (WDM) channels.
Cyclometalated Gold(III) Complexes Containing N‐Heterocyclic Carbene Ligands Engage Multiple Anti‐Cancer Molecular Targets (2017)

Metal N-heterocyclic carbene (NHC) complexes are a promising class of anti-cancer agents displaying potent in vitro and in vivo activities. Taking a multi-faceted approach employing two clickable photoaffinity probes, herein we report the identification of multiple molecular targets for anti-cancer active pincer gold(III) NHC complexes. These complexes display potent and selective cytotoxicity against cultured cancer cells and in vivo anti-tumor activities in mice bearing xenografts of human cervical and lung cancers. Our experiments revealed the specific engagement of the gold(III) complexes with multiple cellular targets, including HSP60, vimentin, nucleophosmin, and YB-1, accompanied by expected downstream mechanisms of action. Additionally, Pt<sup>II</sup> and Pd<sup>II</sup> analogues can also bind the cellular proteins targeted by the gold(III) complexes, uncovering a distinct pincer cyclometalated metal-NHC scaffold in the design of anti-cancer metal medicines with multiple molecular targets.
A ring expansion approach to pyranosylated indolocarbazoles (1995)

A ring expansion protocol has been developed that allows efficient access to pyranosylated indolocarbazoles that are suited for further elaboration to staurosporine and its congeners.
Bioengineering commensal bacteria‐derived outer membrane vesicles for delivery of biologics to the gastrointestinal and respiratory tract (2019)

Gram-negative bacteria naturally produce and secrete nanosized outer membrane vesicles (OMVs). In the human gastrointestinal tract, OMVs produced by commensal Gram-negative bacteria can mediate interactions amongst host cells (including between epithelial cells and immune cells) and maintain microbial homeostasis. This OMV-mediated pathway for host-microbe interactions could be exploited to deliver biologically active proteins to the body. To test this we engineered the Gram-negative bacterium <i>Bacteroides thetaiotaomicron</i> (Bt), a prominent member of the intestinal microbiota of all animals, to incorporate bacteria-, virus- and human-derived proteins into its OMVs. We then used the engineered Bt OMVs to deliver these proteins to the respiratory and gastrointestinal (GI)-tract to protect against infection, tissue inflammation and injury. Our findings demonstrate the ability to express and package both <i>Salmonella enterica</i> ser. Typhimurium-derived vaccine antigens and influenza A virus (IAV)-derived vaccine antigens within or on the outer membrane of Bt OMVs. These antigens were in a form capable of eliciting antigen-specific immune and antibody responses in both mucosal tissues and systemically. Furthermore, immunisation with OMVs containing the core stalk region of the IAV H5 hemagglutinin from an H5N1 strain induced heterotypic protection in mice to a 10-fold lethal dose of an unrelated subtype (H1N1) of IAV. We also showed that OMVs could express the human therapeutic protein, keratinocyte growth factor-2 (KGF-2), in a stable form that, when delivered orally, reduced disease severity and promoted intestinal epithelial repair and recovery in animals administered colitis-inducing dextran sodium sulfate. Collectively, our data demonstrates the utility and effectiveness of using Bt OMVs as a mucosal biologics and drug delivery platform technology.
Epoxide-functionalization of polyethyleneimine for synthesis of stable carbon dioxide adsorbent in temperature swing adsorption (2016)

Amine-containing adsorbents have been extensively investigated for post-combustion carbon dioxide capture due to their ability to chemisorb low-concentration carbon dioxide from a wet flue gas. However, earlier studies have focused primarily on the carbon dioxide uptake of adsorbents, and have not demonstrated effective adsorbent regeneration and long-term stability under such conditions. Here, we report the versatile and scalable synthesis of a functionalized-polyethyleneimine (PEI)/silica adsorbent which simultaneously exhibits a large working capacity (2.2 mmol g(-1)) and long-term stability in a practical temperature swing adsorption process (regeneration under 100% carbon dioxide at 120 °C), enabling the separation of concentrated carbon dioxide. We demonstrate that the functionalization of PEI with 1,2-epoxybutane reduces the heat of adsorption and facilitates carbon dioxide desorption (>99%) during regeneration compared with unmodified PEI (76%). Moreover, the functionalization significantly improves long-term adsorbent stability over repeated temperature swing adsorption cycles due to the suppression of urea formation and oxidative amine degradation.
Effect of Anaerobic Cecal Microflora and Dietary Lactose on Colonization Resistance of Layer Chicks to Invasive Salmonella enteritidis (1991)

The effect of oral inoculation with anaerobic cultures of cecal microflora and providing lactose in the feed on colonization resistance to invasive Salmonella enteritidis was evaluated in newly hatched leghorn chicks. Salmonella colonization of the ceca, tissue invasion and organ colonization, horizontal transmission, and seroconversion were significantly decreased (P less than 0.01) in chicks inoculated with cecal flora. The addition of lactose to the feed, in the absence of cecal microflora, failed to provide protection. Dietary lactose enhanced colonization resistance in chicks that were inoculated with anaerobic cultures of cecal flora. The results indicated that establishment of normal cecal flora in layer chicks together with the addition of lactose to the diet markedly increases resistance to cecal colonization and organ invasion, and decreases horizontal transmission of S. enteritidis.
Sequence and structural analysis of surface protein antigen I/II (SpaA) of Streptococcus sobrinus (1991)

Streptococcal antigen I/II or the surface protein antigen A (SpaA) of Streptococcus sobrinus is an adhesin which mediates binding of the organism to tooth surfaces. The complete sequence of the gene which encodes SpaA has been determined. The gene consists of 4,584 bp and encodes a protein of 1,528 amino acid residues. The deduced amino acid sequence shows extensive homology with those of the cell surface adhesins from Streptococcus mutans serotypes c and f and from Streptococcus sanguis. Structural analysis of the N-terminal region (residues 50 to 550), which is rich in alanine and includes four tandem repeats of an 82-residue sequence, suggests that it adopts an alpha-helical coiled-coil conformation. Cell surface hydrophobicity may be associated with this region. The C-terminal region is more conserved and includes two tandem repeats of a 39-residue proline-rich sequence. A further proline-rich sequence in this region is predicted to span the cell wall. Although a hydrophobic sequence is present in the C-terminal region, it appears to be too short to span the cell membrane. Anchoring of SpaA in the cell membrane may therefore require some form of posttranslational modification or association with another membrane protein.
In vivo evaluation of additively manufactured multi-layered scaffold for the repair of large osteochondral defects (2022)

The online version contains supplementary material available at 10.1007/s42242-021-00177-w.
Scanning electrochemical microscopy. 21. Constant-current imaging with an autoswitching controller (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTScanning electrochemical microscopy. 21. Constant-current imaging with an autoswitching controllerDavid O. Wipf, Allen J. Bard, and Dennis E. TallmanCite this: Anal. Chem. 1993, 65, 10, 1373–1377Publication Date (Print):May 15, 1993Publication History Published online1 May 2002Published inissue 15 May 1993https://doi.org/10.1021/ac00058a013RIGHTS & PERMISSIONSArticle Views285Altmetric-Citations74LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (4 MB) Get e-Alerts Get e-Alerts
Routine Coronary Calcium Scan Can Precisely Measure Vertebral Bone Density Without a Quantitative Calibration Phantom (2015)

The thoracic BMD can be assessed accurately and precisely using QCT and phantomless with a routine CACS.
A peer-to-peer video-on-deniand system using multiple description coding and server diversity (2005)

We propose a video streaming system that uses many ordinary computers (peers) as servers, which is less costly and more scalable than using a single or a few dedicated servers. To circumvent frequent peer disconnects and limited peer up-link bandwidth, each video is coded into multiple descriptions, which are distributed over multiple peers. The system serves a client request by streaming multiple descriptions of the requested video from separate peers. Using the MD-FEC coding scheme, and simple models for peer disconnects, reconnects and for searching replacement serving peers after a peer disconnect, we simulated video streaming in such a system. Our results show significant benefits in using a large number of descriptions and serving peers.
Quantitative Evaluation of Transform Domains for Compressive Sampling-Based Recovery of Sparsely Sampled Volumetric OCT Images (2012)

Recently, compressive sampling has received significant attention as an emerging technique for rapid volumetric imaging. We have previously investigated volumetric optical coherence tomography (OCT) image acquisition using compressive sampling techniques and showed that it was possible to recover image volumes from a subset of sampled images. Our previous findings used the multidimensional wavelet transform as the domain of sparsification for recovering OCT image volumes. In this report, we analyzed and compared the potential and efficiency of three other image transforms to reconstruct the same volumetric OCT image. Two quantitative measures, the mean square error and the structural similarity index, were applied to compare the quality of the reconstructed volumetric images. We observed that fast Fourier transformation and wavelet both are capable of reconstructing OCT image volumes for the orthogonal sparse sampling masks used in this report, but with different merits.
Activity of subcutaneous interleukin-12 in AIDS-related Kaposi sarcoma (2006)

Abstract Interleukin-12 (IL-12) enhances Th1-type T-cell responses and exerts antiangiogenic effects. We initiated a phase 1 pilot study of IL-12 in 32 patients with acquired immunodeficiency syndrome (AIDS)–related Kaposi sarcoma (KS) whose KS was progressing while on antiretroviral therapy. Fifteen patients had poor prognosis T1S1 disease. IL-12 was administered subcutaneously twice weekly at doses from 100 to 625 ng/kg. The maximum tolerated dose was 500 ng/kg, and the principal toxicities were flulike symptoms, transaminase or bilirubin elevations, neutropenia, hemolytic anemia, and depression. No tumor responses were seen at the lowest dose (100 ng/kg), but 17 of 24 evaluable patients at the higher doses had partial or complete responses (response rate, 71%; 95% confidence interval, 48%-89%). Only 3 of 17 patients had a change in antiretroviral therapy before responding, and there were no significant differences between responders and nonresponders with regard to changes in CD4 counts or viral loads. Patients had increases in their serum IL-12, interferon-γ, and inducible protein-10 (IP-10) after the first dose, and increases above baseline persisted after week 4. These results provide preliminary evidence that IL-12 has substantial activity against AIDS-related KS with acceptable toxicity and warrants further investigation for this indication.
Sirtuin7 oncogenic potential in human hepatocellular carcinoma and its regulation by the tumor suppressors MiR-125a-5p and MiR-125b (2012)

Our findings suggest the oncogenic potential of SIRT7 in hepatocarcinogenesis. A regulatory loop is proposed whereby SIRT7 inhibits transcriptional activation of p21(WAF1/Cip1) by way of repression of miR-125a-5p and miR-125b. This makes SIRT7 a promising target in cancer therapy. (HEPATOLOGY 2013).
Speaker Recognition Using e–Vectors (2018)

Systems based on i-vectors represent the current state-of-the-art in text-independent speaker recognition. Unlike joint factor analysis (JFA), which models both speaker and intersession subspaces separately, in the i-vector approach all the important variability is modeled in a single low-dimensional subspace. This paper is based on the observation that JFA estimates a more informative speaker subspace than the “total variability” i- vector subspace, because the latter is obtained by considering each training segment as belonging to a different speaker. We propose a speaker modeling approach that extracts a compact representation of a speech segment, similar to the speaker factors of JFA and to i-vectors, referred to as “e-vector.” Estimating the e-vector subspace follows a procedure similar to i-vector training, but produces a more accurate speaker subspace, as confirmed by the results of a set of tests performed on the NIST 2012 and 2010 Speaker Recognition Evaluations. Simply replacing the i-vectors with e-vectors we get approximately 10% average improvement of the C <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">primary</sub> cost function, using different systems and classifiers. It is worth noting that these performance gains come without any additional memory or computational costs with respect to the standard i-vector systems.
Visual Analysis of North Atlantic Hurricane Trends Using Parallel Coordinates and Statistical Techniques (2008)

The integration of automated statistical analysis capabilities with a highly interactive, multivariate visualization interface is presented in this paper. Innovative visual interaction techniques such as dynamic axis scaling, conjunctive parallel coordinates, statistical indicators, and aerial perspective shading are exploited to enhance the utility of classical parallel coordinate plots. Moreover, the system facilitates statistical processes such as stepwise regression and correlation analysis to assist in the identification and quantification of the most significant predictors for a particular dependent variable. These capabilities are combined into a unique visualization system that is demonstrated via a North Atlantic hurricane climate study using a systematic workflow. This research corroborates the notion that enhanced parallel coordinates coupled with statistical analysis can be used for more effective knowledge discovery and confirmation in complex, real-world data sets.