gebhart/patrepeval huggingface dataset:
Binding of a highly potent protease‐activated receptor‐2 (PAR2) activating peptide, [<sup>3</sup>H]2‐furoyl‐LIGRL‐NH<sub>2</sub>, to human PAR2 (2005)

1 To determine the binding characteristics of a highly potent agonist for protease-activated receptor-2 (PAR2), 2-furoyl-Leu-Ile-Gly-Arg-Leu-amide (2-furoyl-LIGRL-NH(2)), whole-cell binding assays were performed utilising a radioactive ligand, [(3)H]2-furoyl-LIGRL-NH(2). 2 Specific binding of [(3)H]2-furoyl-LIGRL-NH(2) was observed in NCTC2544 cells, dependent upon PAR2 expression, and competitively displaced by the addition of unlabeled PAR2 agonists. Scatchard analysis of specific saturation binding suggested a single binding site, with K(d) of 122+/-26.1 nM and a corresponding B(max) of 180+/-6 f mol in 3.0 x 10(5) cells. 3 The relative binding affinities of a series of modified PAR2 agonist peptides obtained from competition studies paralleled their relative EC(50) values for Ca(2+) mobilisation assays, indicating improved binding affinities by substitution with 2-furoyl at the N-terminus serine. 4 Pretreatment of cells with trypsin reduced specific binding of [(3)H]2-furoyl-LIGRL-NH(2), demonstrating direct competition between the synthetic agonist peptide and the proteolytically revealed tethered ligand for the binding site of the receptor. 5 In HCT-15 cells endogenously expressing PAR2, the binding of [(3)H]2-furoyl-LIGRL-NH(2) was displaced by addition of unlabeled ligands, Ser-Leu-Ile-Gly-Lys-Val (SLIGKV-OH) or 2-furoyl-LIGRL-NH(2). The relative binding affinity of 2-furoyl-LIGRL-NH(2) to SLIGKV-OH was comparable to its relative EC(50) value for Ca(2+) mobilisation assays. 6 The binding assay was successfully performed in monolayers of PAR2 expressing NCTC2544 and human umbilical vein endothelial cells (HUVEC), in 96- and 24-well plate formats, respectively. 7 These studies indicate that [(3)H]2-furoyl-LIGRL-NH(2) binds to human PAR2 at its ligand-binding site. The use of this radioligand will be valuable for characterising chemicals that interact to PAR2.
Photodynamic Treatment of Red Blood Cell Concentrates For Virus Inactivation Enhances Red Blood Cell Aggregation: Protection with Antioxidants (1997)

Photodynamic treatment (PDT) using phthalocyanines and red light appears to be a promising procedure for decontamination of red blood cell (RBC) concentrates for transfusion. A possible complication of this treatment may be induced aggregation of RBC. The production of RBC aggregates was measured with a novel computerized cell flow properties analyzer (CFA). The PDT of RBC concentrates with sulfonated aluminum phthalocyanine (AIPcS4) and the silicon phthalocyanine Pc 4 under virucidal conditions markedly enhanced RBC aggregation and higher shear stress was required to disperse these aggregates. The clusters of cells were huge and abnormally shaped, unlike the rouleaux formed by untreated RBC. This aggregation was prevented when a mixture of antioxidants was included during PDT. Addition of the antioxidants after PDT reduced aggregation only partially. It is concluded that inclusion of antioxidants during PDT of RBC concentrates prior to transfusion may reduce or eliminate the hemodynamic risk that the virucidal treatment may present to the recipient.
ANALYSIS OF A NOVEL A-KINASE ANCHORING PROTEIN 100, (AKAP 100) (1995)

Conference Article| May 01 1995 ANALYSIS OF A NOVEL A-KINASE ANCHORING PROTEIN 100, (AKAP 100) Shirley McCartney; Shirley McCartney §Vollum Institute for Advanced Biomedical Research, Oregon Health Sciences University, L-474, 3181 S. W. Sam Jackson Park Road, Portland OR. 97201 U.S.A. Search for other works by this author on: This Site PubMed Google Scholar Brian M. Little; Brian M. Little †Cascade Oncogenics, Portland OR. 97201 U.S.A. Search for other works by this author on: This Site PubMed Google Scholar John D. Scott John D. Scott §Vollum Institute for Advanced Biomedical Research, Oregon Health Sciences University, L-474, 3181 S. W. Sam Jackson Park Road, Portland OR. 97201 U.S.A. Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1995) 23 (2): 268S. https://doi.org/10.1042/bst023268s Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn MailTo Cite Icon Cite Get Permissions Citation Shirley McCartney, Brian M. Little, John D. Scott; ANALYSIS OF A NOVEL A-KINASE ANCHORING PROTEIN 100, (AKAP 100). Biochem Soc Trans 1 May 1995; 23 (2): 268S. doi: https://doi.org/10.1042/bst023268s Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search This content is only available as a PDF. © 1995 Biochemical Society1995 Article PDF first page preview Close Modal You do not currently have access to this content.
An Innovator in Blended Learning mode of Education (2017)

Prof. Manoj Kulshrestha, Ph.D from Indian Institute of Technology (IIT), Delhi, New Delhi is Professor of Civil Engineering in School of Engineering &amp; Technology (SOET) at Indira Gandhi National Open University (IGNOU), New Delhi, where he teaches Construction Project Management. Additionally, he is Director of National Centre for Innovation in Distance Education (NCIDE).
Notice of Violation of IEEE Publication Principles Nuclear Localization of HIV-1 Tat Functionalized Gold Nanoparticles (2007)

The impermeable nature of the cell plasma membrane limits the therapeutic uses of many macromolecules and there is therefore a growing effort to circumvent this problem by designing strategies for targeted intracellular delivery. During the last decade several cell penetrating peptides, such as the HIV-1 tat peptide, have been shown to traverse the cell membrane, where integral protein transduction domains (PTDs) are responsible for their cellular uptake, and to reach the nucleus while retaining biological activity. It has since been discovered that PTDs can enable the cellular delivery of conjugated biomolecules and even nanoparticles, but nuclear delivery has remained problematic. This present study focuses on the development of water soluble, biocompatible gold nanoparticles of differing size functionalized with the HIV-1 tat PTD with the aim of producing nuclear targeting agents. The particles were subsequently tested in vitro with a human fibroblast cell line, with results demonstrating successful nanoparticle transfer across the plasma membrane, with 5 nm particles achieving nuclear entry while larger 30 nm particles are retained in the cytoplasm, suggesting entry is blocked via nuclear pores dimensions.
Early evaluation for performance enhancement in phased logic (2005)

Data-dependent completion time is a well-known advantage of self-timed circuits, one that allows them to operate at average rather than worst-case execution rates. A technique called early evaluation (EE) that extends this advantage by allowing self-timed modules to produce results before all of their inputs have arrived is described here. The technique can be applied to any combinational function and is integrated into the phased logic (PL) design methodology that accepts synchronous design entry and produces delay-insensitive self-timed circuits. We describe an algorithm that ensures that the resulting delay-insensitive circuits are safe, and develop a generalized method for inserting EE gates into any PL netlist. We give performance results for several benchmark circuits, including a five-stage pipelined CPU and a microprogrammed floating-point unit. Comparisons are made among clocked circuits, PL circuits, and PL circuits with EE. Simulation results show a clear performance benefit for PL circuits that use EE.
<i>Acinetobacter baumannii</i> 2002–2008: Increase of Carbapenem-Associated Multiclass Resistance in the United States (2010)

The study consisted of data for 55,330 U.S. Acinetobacter baumannii isolates from The Surveillance Network(®) database for the period 2002-2008. Risk factors were time, age, sex, census region, location (Ward or ICU), and isolate source. Antimicrobial susceptibility data were available for carbapenems, cephalosporins, aminoglycosides, fluoroquinolones, and β-lactam/β-lactamase inhibitor combinations. Multiclass resistance was defined as nonsusceptibility to carbapenems and two or more additional classes. Odds of resistance were obtained using a logistic regression model with cubic splines. Carbapenem-associated multiclass resistance has had a 3.7-fold (95% confidence interval [CI] 3.4-4.3) increase from 20.6% in 2002 to 49.2% in 2008. Among blood isolates the increase was by 2.2 times (95% CI 1.7-2.9). Subjects <18 years old had significantly (p < 0.001) lower rates in 2002 (6.9%) than those 65 years or older (21.5%), but by 2008 this difference diminished as rates increased to 44.2% and 54.2%, respectively. A similar divergence was also observed between ICU and Ward, with no differences in 2002, whereas in 2008 ICU isolates had significantly higher rates (55.2%, 95% CI 53.6%-56.9%) than Ward isolates (45.6%, 95% CI 44.2%-47.0%). Over half of all A. baumannii-resistant isolates were carbapenem and multiclass resistant in 2008. Rates among subjects <18 years old have increased faster than those of the elderly, and in the ICU as compared to Ward.
Inhalational Gases for Neuroprotection in Traumatic Brain Injury (2021)

Despite multiple prior pharmacological trials in traumatic brain injury (TBI), the search for an effective, safe, and practical treatment of these patients remains ongoing. Given the ease of delivery and rapid absorption into the systemic circulation, inhalational gases that have neuroprotective properties will be an invaluable resource in the clinical management of TBI patients. In this review, we perform a systematic review of both pre-clinical and clinical reports describing inhalational gas therapy in the setting of TBI. Hyperbaric oxygen, which has been investigated for many years, and some of the newest developments are reviewed. Also, promising new therapies such as hydrogen gas, hydrogen sulfide gas, and nitric oxide are discussed. Moreover, novel therapies such as xenon and argon gases and delivery methods using microbubbles are explored.
40.5L: <i>Late‐News Paper:</i> TFT‐LCD with RGBW Color System (2003)

Abstract Although LCDs are widely used in power‐consumption critical applications, they are not very energy‐efficient. The largest absorber of light is the color filter. We developed a TFT‐LCD with four‐color subpixels: red, green, blue and white. The addition of the white subpixel greatly enhanced the light efficiency: up by 50%. The RGB‐to‐RGBW mapping algorithm was designed so that the hue and saturation remains unchanged from the original color. The result is a very bright display with color characteristic almost identical to RGB LCDs.
The organic chemistry of aliphatic nitrogen compounds (1995)

1: Amines. 2: Enamines and ynamines. 3: Imines. 4: Nitriles (cyanides). 5: N-oxides and nitrones. 6: Alkyl isonitriles (isocyanides). 7: Cyanates, isocyanates, thiocyanates, and isothiocyanates. 8: Amides, thiomides, and carbamates (urethanes). 9: Ureas, thioureas, carbodiimides. 10: Amidines. 11: Nitroalkanes and nitroalkenes. 12: N-Nitroso compounds : nitrosamines, nitrosamides, nitrosoureas, and nitrosourethanes. 13: Diazoalkanes. 14: Hydroxylamines. 15: Hydrazines. 16: Azo and azoxy compounds. 17: Azides. 18: Alkyl nitrates and nitrates. 19: Nitramines. 20: B-Amino alcohols. 21: Aziridines. 22: B-Lactams
PROLOGUS (2012)

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Structure−Activity Relationship Studies of Ethyl 2-Amino-6-bromo-4-(1-cyano-2-ethoxy-2-oxoethyl)-4<i>H</i>-chromene-3-carboxylate (HA 14-1), an Antagonist for Antiapoptotic Bcl-2 Proteins To Overcome Drug Resistance in Cancer (2006)

The structure-activity relationship studies of ethyl 2-amino-6-cyclopentyl-4-(1-cyano-2-ethoxy-2-oxoethyl)-4H-chromene-3-carboxylate (1, HA 14-1), an antagonist of the antiapoptotic Bcl-2 proteins, are reported. A series of analogues of 1 with varied functional groups at the 6-position of the chromene ring were synthesized. These candidates were evaluated for their binding interactions with three antiapoptotic proteins: Bcl-2, Bcl-XL, and Bcl-w. They were also assayed for their in vitro cytotoxicities against a set of Jurkat cells with varied levels of Bcl-2 and Bcl-XL proteins and a non-small-cell lung carcinoma cell line (NCI-H460). It was found that the 6-bromo of 1 was not essential for its bioactivity and the 6-position can accommodate a variety of alkyl groups. 1 and its analogues bind to all of the three antiapoptotic Bcl-2 proteins tested. Positive correlations were observed between the binding affinities of these candidates to the antiapoptotic Bcl-2 proteins and their in vitro cytotoxicities, suggesting that the antiapoptotic Bcl-2 proteins are likely to be the cellular targets of 1 and its analogues. (In this study, the binding interactions of the small molecules to antiapoptotic Bcl-2 proteins were studied by assaying their abilities to compete against a Bak peptide binding to the antiapoptotic Bcl-2 proteins. Inhibitory constants, instead of dissociation constants, were obtained in such assays. The term "binding affinity" is used in this article for simplicity.) The most active compound, 3g, had a >3-fold increase of binding affinity to the antiapoptotic Bcl-2 proteins and a >13-fold increase of in vitro cytotoxicity over 1. Though Jurkat cells with transgenic overexpression of Bcl-2 or Bcl-XL protein can develop resistance to standard cancer therapies, such cells failed to develop resistance to 1 based candidates. 1 also sensitizes Jurkat cells to cisplatin. These studies provide further support that 1 and its analogues function as antagonists for antiapoptotic Bcl-2 proteins and that they have the potential, either as a single agent or as a combination therapy with other anticancer agents, to treat cancers with the overexpression of antiapoptotic Bcl-2 proteins.
Quantum cascade laser on silicon (2016)

The mid-infrared spectral region, 2–20 μm, is of great interest for sensing and detection applications, in part because the vibrational transition energies of numerous molecules fall in that region. Silicon photonics is a promising technology to address many of these applications on a single integrated, low-cost platform. Near-infrared light sources, heterogeneously integrated on silicon, have existed for more than a decade, and there have been numerous incorporations of mid-infrared optical devices on silicon platforms. However, no lasers fully integrated onto silicon have previously been demonstrated for wavelengths longer than 2.0 μm. Here we report, to the best of our knowledge, the first quantum cascade lasers on silicon emitting 4.8 μm light, integrated with silicon-on-nitride-on-insulator (SONOI) waveguides, and operating in pulsed mode at room temperature. The broadband and versatile nature of both quantum cascade lasers and the SONOI platform suggests that this development can be expanded to build photonic integrated circuits throughout the near- and mid-infrared on the same chip.
A Gold(III) Pincer Ligand Scaffold for the Synthesis of Binuclear and Bioconjugated Complexes: Synthesis and Anticancer Potential (2018)

Cyclometalated (C^N^C)Au<sup>III</sup> complexes bearing functionalized N-heterocyclic carbene (NHC) ligands provide a high-yielding, modular route to bioconjugated and binuclear complexes. This methodology has been applied to the synthesis of bioconjugated complexes presenting biotin and 17α-ethynylestradiol vectors, as well as to the synthesis of bimetallic Au<sup>III</sup> /Au<sup>I</sup> complexes. The in vitro antiproliferative activities of these compounds against various cancer cells lines depend on the linker length, with the longer linker being the most potent. The estradiol conjugate AuC<sub>6</sub> Estra proved to be more toxic against the estrogen receptor positive (ER+) cancer cells than against the ER- cancer cells and non-cancer cells. The bimetallic complex AuC<sub>6</sub> Au was more selective for breast cancer cells with respect to a healthy cell standard than the monometallic complex AuNHC. The metal uptake study on cells expressing or not biotin and estrogen receptors revealed an improved and targeted delivery of gold for both the bioconjugated complexes AuC<sub>6</sub> Biot and AuC<sub>6</sub> Estra compared to the non-vectorised analogue AuNHC. The investigations of the interaction of the bioconjugates and bimetallic complexes with human telomeric G-quadruplex DNA using FRET-melting techniques revealed a reduced ability to stabilize this DNA structure with respect to the non-vectorised analogue AuNHC.
On-line Inertia Measurement of Unmanned Aerial Vehicles using on board Sensors and Bifilar Pendulum (2018)

Identification of a dynamical model and its parameters is one of the fundamental problems in the field of robotics and system dynamics modelling. For the general situation of an object motion with six degrees of freedom (6-DOF), such as in the case of the Unmanned Aerial Vehicle (UAV), the key physical parameters are vehicle mass and moment of inertia. Even though UAV mass and its geometry/topology are easily obtainable, it is difficult to identify the inertia tensor considering that it is not measurable by static tests. This article presents a simple and effective method for on-line estimation of a rigid-body inertia based on a two-wire pendulum and an on-board integrated sensor system. Herein, the test subject (i.e. UAV) is suspended by two thin parallel wires in such a way to form a bifilar torsional pendulum about the vertical axis. Using on-board sensors from the UAV flight controller (FC) unit, the pendulum oscillations are recorded and processed in order to obtain trend-free and noise-free signals used in the final inertia estimation phase. The proposed identification algorithm is verified experimentally for two typical cases of a suspended objects related to the UAV control box and a full UAV configuration.
Coherencia entre el pensar y el hacer: la concepción de los docentes acerca de la comprensión lectora y su aplicación en el aula (2018)

espanolLas habilidades trabajadas en la comprension de lectura son desarrolladas por los estudiantes, principalmente por las estrategias que les entrega el(la) profesor(a). Es importante que los docentes manejen un concepto de comprension lectora, puesto que esto debiese mostrar que habilidades son propias de ser desarrolladas en sus clases sobre el tema y que elementos deben ser considerados (texto, lector, contexto).Es de acuerdo a este concepto, el de comprension, que los docentes pueden aplicar metodologias que deben desarrollar en sus clases y que pueden ser encasilladas en diversos modelos (el linguistico, psicolinguistico, sociocultural).Si los docentes establecen un concepto de comprension y lo aplican en sus clases, las practicas pedagogicas pueden ser mejoradas, ya que mostrarian mayor coherencia en la aplicacion. EnglishStudents’ reading comprehension skills are primarily developed by the strategies provided to them by the teacher. It is important that teachers share a concept of reading comprehension since this will show which skills are to be developed in their classes on the topic and what elements should be taken into account (text, reader and context).It is according to the concept of reading comprehension that teachers can apply methodologies employed in their classrooms and that can be categorized into various models (the linguistic, psycholinguistic, or sociocultural one).If teachers have a concept of comprehension and apply it in their classes, their pedagogical practices could be improved since they would show more coherence in the application.
An orally active inhibitor of leukotriene synthesis accelerates healing in a rat model of colitis (1990)

Leukotrienes (LTs) have been implicated as mediators of the inflammation and ulceration associated with ulcerative colitis and Crohn's disease. In the present study, the effects of a novel, orally active inhibitor of LT synthesis (MK-886) were examined in a rat model of chronic colitis. Colitis was induced by intracolonic administration of trinitrobenzenesulfonic acid. Colonic LTB4 synthesis was measured after incubation of tissue samples in vitro and by in vivo equilibrium dialysis. A single dose of MK-886 (10 mg/kg) significantly inhibited colonic LTB4 synthesis for greater than 24 h. Daily treatment with this dose significantly reduced colonic damage, as assessed macroscopically and histologically, when the treatment was performed 2 h before induction of colitis and daily thereafter for 1 wk, but not when treatment was performed during the second week after induction of colitis. A less marked beneficial effect of MK-886 was observed when the pretreatment dose was excluded, suggesting a role for LTs in the early events of the inflammatory process. Inhibition of LT synthesis during the first 24 h after induction of colitis did not alter the extent of infiltration of neutrophils into the colon, as measured by tissue myeloperoxidase activity. Daily treatment with sulfasalazine (100 mg/kg po) either during the first or second week after induction of colitis did not significantly affect the rates of healing. At the dose used, sulfasalazine only produced a transient inhibition of colonic LTB4 synthesis. This study therefore demonstrates that a specific, orally active inhibitor of LT synthesis can significantly accelerate healing in this animal model of colitis when the treatment is performed during the early phase of the inflammatory response.
[HBV transmission from father to foetus and HBV DNA in tissues outside the liver]. (1999)

HBV transmission from father to foetus may be present. HBV DNA in tissues outside liver of foetus can be detected.
THE RELATIONSHIP OF PSYCHIATRIC ILLNESS WITH GASTROINTESTINAL DISEASE (1995)

Higher rates of psychiatric comorbidity as well as more impaired psychosocial adjustment occur with the functional bowel disorders and are particularly high in self-selected referral patients compared with community populations. Reciprocally, some studies show higher rates of functional bowel disturbances in patients with psychiatric diagnoses. Remaining alert for and addressing coexisting psychiatric illness will enhance treatment outcome (increased patient compliance, functioning, and satisfaction). Additionally, psychological factors affect the clinical expression of structural disease. Resetting treatment goals from cure to coping with chronic illness and setting personal limits are important.
Application of discrete-event simulation in health care clinics: A survey (1999)

In recent decades, health care costs have dramatically increased, while health care organisations have been under severe pressure to provide improved quality health care for their patients. Several health care administrators have used discrete-event simulation as an effective tool for allocating scarce resources to improve patient flow, while minimising health care delivery costs and increasing patient satisfaction. The rapid growth in simulation software technology has created numerous new application opportunities, including more sophisticated implementations, as well as combining optimisation and simulation for complex integrated facilities. This paper surveys the application of discrete-event simulation modeling to health care clinics and systems of clinics (for example, hospitals, outpatient clinics, emergency departments, and pharmacies). Future directions of research and applications are also discussed.