gebhart/patrepeval huggingface dataset:
Isolation of Chinese hamster ovary cell lines temperature conditional for the cell-surface expression of integral membrane glycoproteins. (1989)

A procedure is described to select mutants of Chinese hamster ovary cells that are conditionally defective for the cell-surface expression of integral membrane glycoproteins, including the hemagglutinin (HA) of influenza virus. Using a combination of cell sorting and biochemical screening, seven cell lines were obtained that express more cell-surface HA at 32 degrees C than at 39 degrees C. The production of infectious vesicular stomatitis virus, whose growth requires insertion of an integral membrane protein into the plasma membrane, was also temperature conditional in the majority of these mutant cell lines. Five of the lines synthesized apparently normally core-glycosylated HA at the elevated temperature but the protein was neither displayed on the cell surface nor accumulated intracellularly. In these cell lines, little or no terminally glycosylated HA molecules were observed after synthesis at 39 degrees C. By contrast, the core glycosylation of HA and several other integral membrane proteins was abnormal in the remaining two cell lines at both permissive and restrictive temperatures, due to a lesion in a cellular gene(s) that affects the formation of and/or the addition of mannose-rich oligosaccharide chains to newly synthesized polypeptides. Although HA was transported to the plasma membrane at both 32 and 39 degrees C, it did not accumulate on the cell surface at the higher temperature, apparently because of an increased rate of degradation.
E-platooning: Optimizing platoon formation for long-haul transportation with electric commercial vehicles (2022)

Recent technological developments allow to connect vehicles via digital data transmission, so that a queue of trucks can safely be driven in close proximity. The lower air resistance of these truck platoons decreases fuel consumption and, thus, contributes to reducing the carbon footprint of road-based freight transportation. The efficiency of platooning depends not only on the aerodynamic drag, which considerably reduces with decreasing inter-vehicle distance. Whether a suitable platooning partner can be found also depends on the trucks’ willingness-to-wait and on a successful platoon formation process. Electric vehicles have to wait while loading their batteries anyway. Therefore, it might be possible to successfully coordinate charging with waiting for partner trucks. Furthermore, driving in a platoon reduces energy consumption, so that electric trucks can increase their driving ranges, which gives them an additional motive for platooning. This paper provides optimization approaches to schedule the platoon formation process of electric commercial vehicles via a central platform. Given a set of trucks with predetermined routes and time windows for their on-time arrivals at their destinations, we derive driving, charging and platoon formation schedules, such that total energy costs are minimized. We formulate the problem, analyze its computational complexity, and provide a novel matheuristic. Its suitable performance is documented by extensive computational experiments. Furthermore, we apply our algorithm to quantify the performance gains when platooning electric trucks and benchmark these outcomes with individual driving schedules of electric trucks and with platoons of traditional trucks powered by a combustion engine.
Investigation of the misfueling of reciprocating piston aircraft engines (1988)

The Aircraft Misfueling Detection Project was developed by the Goddard Space Flight Center/Wallops Flight Facility at Wallops Island, Virginia. Its purpose was to investigate the misfueling of reciprocating piston aircraft engines by the inadvertent introduction of jet fuel in lieu of or as a contaminant of aviation gasoline. The final objective was the development of a device(s) that will satisfactorily detect misfueling and provide pilots with sufficient warning to avoid injury, fatality, or equipment damage. Two devices have been developed and successfully tested: one, a small contamination detection kit, for use by the pilot, and a second, more sensitive, modified gas chromatograph for use by the fixed-base operator. The gas chromatograph, in addition to providing excellent quality control of the fixed-base operator's fuel handling, is a very good backup for the detection kit in the event it produces negative results. Design parameters were developed to the extent that they may be applied easily to commercial production by the aircraft industry.
PROGRESS IN VERIFICATION OF SKILLFULLY SIMULATED HANDWRITTEN SIGNATURES (1991)

This paper compares the performances of parametric and reference pattern based features (RPBFs) in the verification of skillfully simulated handwritten signatures. The comparison shows that RPBFs significantly improve results and give about 90% correct verification using only shape features. The performance of the used shape features is independent of the signature shape, language and position in the document. The careful analysis of the experimental results of using RPBFs in verification has led to the conclusion that two-dimensional RPBFs will give much better performance.
Proenkephalin is a nuclear protein responsive to growth arrest and differentiation signals. (1995)

Neuropeptide precursors are traditionally viewed as molecules destined to be cleaved into bioactive peptides, which are then released from the cell to act on target cell surface receptors. In this report we demonstrate nuclear localization of the enkephalin precursor, proenkephalin, in rodent and human embryonic fibroblasts (Swiss 3T3 and MRC-5 cells) and in rodent myoblasts (C2C12 cells). Nuclear proenkephalin, detected by immunofluorescence with a panel of antiproenkephalin monoclonal antibodies, is distributed predominantly in three patterns. Selective abolition of these patterns with salt, nuclease, or methanol is associated with liberation of immunoprecipitable proenkephalin into the extraction supernatant. Proenkephalin antigenic domains, mapped using phage display libraries and synthetic peptides, are differentially revealed in the three distribution patterns. Selective epitope revelation may reflect different conformational forms of proenkephalin or its existence in complexes with other nuclear proteins, forms which therefore have different biochemical associations with the nuclear substructure. In fibroblast cell populations in transition to growth arrest, nuclear proenkephalin responds promptly to mitogen withdrawal and cell-cell contact by transient, virtually synchronous unmasking of multiple antigenic domains in a fine punctate distribution. A similar phenomenon is observed in myoblasts undergoing differentiation. The acknowledgment of growth arrest and differentiation signals by nuclear proenkephalin suggests its integration with transduction pathways mediating these signals. To begin to address the mechanism of nuclear targeting, we have transfected mutated and nonmutated proenkephalin into COS (African green monkey kidney) cells. Nonmutated proenkephalin is localized exclusively in the cytoplasm; however, proenkephalin mutated at the first ATG codon, or devoid of its signal peptide sequence, is targeted to the nucleus as well as to the cytoplasm. From this we speculate that nuclear proenkephalin arises from a primary translation product that lacks a signal peptide sequence because of initiation at a different site.
Urinary Pyridinoline and Deoxypyridinoline as Potential Markers of Bone Metastasis in Patients with Prostate Cancer (1996)

Measurement of urinary pyridinoline and deoxypyridinoline may provide a useful marker of prostate cancer metastatic to bone and may be useful in monitoring the response to treatment.
Predictive Milling of Pharmaceutical Materials Using Nanoindentation of Single Crystals (2004)

Five pharmaceutical materials, including two salts and three neutral compounds, have been subjected to nanoindentation analysis on a single-crystal scale. The nanoindentation experiments were used to calculate a brittleness index for each of the five materials. These results were compared to the size reductions that were obtained on a pilot-plant scale mill. A good correlation between single crystal and large pilot-plant scale results was obtained for the range of materials studied.
Plasmonic Photothermal Gold Bipyramid Nanoreactors for Ultrafast Real-Time Bioassays (2017)

Nucleic acid amplification techniques have been among the most powerful tools for biological and biomedical research, and the vast majority of the bioassays rely on thermocycling that uses time-consuming and expensive Peltier-block heating. Here, we introduce a plasmonic photothermal method for quantitative real-time PCR, using gold bipyramids and light to achieve ultrafast thermocycling. Moreover, we successfully extend our photothermal system to other biological assays, such as isothermal nucleic acid amplification and restriction enzyme digestion.
Cell‐Wall Alterations as an Attribute of<i>Mycobacterium tuberculosis</i>in Latent Infection (2003)

Ziehl-Neelsen (ZN) staining is the key technique for diagnosis of mycobacterial infections; however, a high percentage of patients exhibit positive signs of tuberculosis, as indicated by pathology, culture of mycobacteria, and polymerase chain-reaction analysis, and yet show negative results on ZN staining. In this report we present evidence that such ZN-negative specimens represent Mycobacterium tuberculosis bacilli in a dormant state with distinct cell-wall alterations: the classical cell-wall composition-dependent ZN staining of M. tuberculosis in lung sections gradually discontinued with persistence of infection, both in mice and in human patients; in contrast, detection of mycobacteria by cell-wall composition-independent staining using a polyclonal anti-M. bovis Bacille-Calmette-Guérin serum continued with persistence of infection. These findings have important implications for diagnosis, as well as for both chemotherapy and development of vaccine strategies.'
In-vitro investigation of the hemodynamic responses of the cerebral, coronary and renal circulations with a rotary blood pump installed in the descending aorta (2016)

This study investigates the hemodynamic responses of the cardiovascular system when a rotary blood pump is operating in the descending aorta, with a focus on the cerebral, coronary and renal autoregulation, using our in-house cardiovascular emulator. Several improvements have been made from our previous studies. A novel coronary system was developed to replicate the native coronary perfusion. Three pinch valves actuated by stepper motors were used to simulate the regional autoregulation systems of the native cerebral, coronary and renal circulations. A rotary pump was installed in the descending aorta, in series with the heart, and the hemodynamic responses of the cardiovascular system were investigated with a focus on cerebral, coronary and renal circulation over a wide range of pump rotor speeds. Experiments were performed twice, once with the autoregulation systems active and once with the autoregulation systems inactive, to reflect that there will be some impairment of autoregulatory systems in a patient with heart failure. It was shown that by increasing the rotor speed to 3000 rpm, the cardiac output was improved from 2.9 to 4.1 L/min as a result of an afterload reduction induced by the pressure drop upstream of the pump. The magnitudes of changes in perfusion in the cerebral, coronary and renal circulations were recorded with regional autoregulation systems active and inactive.
Rating of CCl<sub>4</sub>‐induced rat liver fibrosis by blood serum glycomics (2006)

The GlycoTest is much more sensitive than biochemical tests for evaluating liver fibrosis/cirrhosis in the rat model. The test can also be used as a non-invasive marker for screening and monitoring the antifibrotic activity of potential therapeutic compounds.
Meeting report (2001)

Conference (MPSA2000) took place in beautiful Charlottesville, Vir-
Regioselective Synthesis of 2-Amino-Substituted 1,3,4-Oxadiazole and 1,3,4-Thiadiazole Derivatives via Reagent-Based Cyclization of Thiosemicarbazide Intermediate (2012)

A regioselective, reagent-based method for the cyclization reaction of 2-amino-1,3,4-oxadiazole and 2-amino-1,3,4-thiadiazole core skeletons is described. The thiosemicarbazide intermediate 3 was reacted with EDC·HCl in DMSO or p-TsCl, triethylamine in N-methyl-2-pyrrolidone to give the corresponding 2-amino-1,3,4-oxadiazoles 4 and 2-amino-1,3,4-thiadiazoles 5 through regioselcective cyclization processes. The regioselectivity was affected by both R(1) and R(2) in p-TsCl mediated cyclization. It is shown in select sets of thiosemicarbazide 3 with R(1)(benzyl) and R(2)(phenyl). 2-Amino-1,3,4-oxadiazole 4 was also shown in the reaction of p-TsCl mediated cyclization. The resulting 2-amino-1,3,4-oxadiazole and 2-amino-1,3,4-thiadiazole core skeleton are functionalized with various electrophiles such as alkyl halide, acid halides, and sulfornyl chloride in high yields.
Dysregulated iron metabolism in polycythemia vera: etiology and consequences (2018)

Polycythemia vera (PV) is a chronic myeloproliferative neoplasm. Virtually all PV patients are iron deficient at presentation and/or during the course of their disease. The co-existence of iron deficiency and polycythemia presents a physiological disconnect. Hepcidin, the master regulator of iron metabolism, is regulated by circulating iron levels, erythroblast secretion of erythroferrone, and inflammation. Both decreased circulating iron and increased erythroferrone levels, which occur as a consequence of erythroid hyperplasia in PV, are anticipated to suppress hepcidin and enable recovery from iron deficiency. Inflammation which accompanies PV is likely to counteract hepcidin suppression, but the relatively low serum ferritin levels observed suggest that inflammation is not a major contributor to the dysregulated iron metabolism. Furthermore, potential defects in iron absorption, aberrant hypoxia sensing and signaling, and frequency of bleeding to account for iron deficiency in PV patients have not been fully elucidated. Insufficiently suppressed hepcidin given the degree of iron deficiency in PV patients strongly suggests that disordered iron metabolism is an important component of the pathobiology of PV. Normalization of hematocrit levels using therapeutic phlebotomy is the most common approach for reducing the incidence of thrombotic complications, a therapy which exacerbates iron deficiency, contributing to a variety of non-hematological symptoms. The use of cytoreductive therapy in high-risk PV patients frequently works more effectively to reverse PV-associated symptoms in iron-deficient relative to iron-replete patients. Lastly, differences in iron-related parameters between PV patients and mice with JAK2 V617F and JAK2 exon 12 mutations suggest that specific regions in JAK2 may influence iron metabolism by nuanced changes of erythropoietin receptor signaling. In this review, we comprehensively discuss the clinical consequences of iron deficiency in PV, provide a framework for understanding the potential dysregulation of iron metabolism, and present a rationale for additional therapeutic options for iron-deficient PV patients.
Stereo Matching with Color and Monochrome Cameras in Low-Light Conditions (2016)

Consumer devices with stereo cameras have become popular because of their low-cost depth sensing capability. However, those systems usually suffer from low imaging quality and inaccurate depth acquisition under low-light conditions. To address the problem, we present a new stereo matching method with a color and monochrome camera pair. We focus on the fundamental trade-off that monochrome cameras have much better light-efficiency than color-filtered cameras. Our key ideas involve compensating for the radiometric difference between two cross-spectral images and taking full advantage of complementary data. Consequently, our method produces both an accurate depth map and high-quality images, which are applicable for various depth-aware image processing. Our method is evaluated using various datasets and the performance of our depth estimation consistently outperforms state-of-the-art methods.
Colloid-chemical properties of polymeric complexes based on polycarboxylic acids and polyacrylamide (2003)

Colloid-chemical properties of polymeric complexes based on polyacrylic acid, polymethacrylic acid, and copolymer of methacrylic acid and pinene with polyacrylamide in diluted aqueous solutions have been investigated. The synthesis of the copolymer of methacrylic acid and pinene, which is more hydrophobic than polyacrylic and polymethacrylic acids, is described. With the addition of polyacrylamide, macromolecules of polycarboxylic acids in aqueous solutions interact by means of hydrogen bonds between carboxylic and amide groups. Intermolecular interactions depend on conformational mobility of linear parts of the macromolecular chains and determine the complexation ability. The effect of the hydrophobicity of the initial polymers and their ratio on the properties (viscosity, pH and surface tension) of the polymeric complexes is discussed. The stability of the complexes formed depends on the presence and quantity of hydrophobic groups in the macromolecules of the polyacids.
Lactosylceramide recruits PKCα/ε and phospholipase A<sub>2</sub>to stimulate PECAM-1 expression in human monocytes and adhesion to endothelial cells (2004)

Despite the importance of platelet/endothelial cell adhesion molecule-1 (PECAM-1, CD31) in the adhesion and diapedesis of monocytes/lymphocytes, little is known about the mechanisms by which it is regulated. We explored the role of a glycosphingolipid, lactosylceramide (LacCer), in modulating PECAM-1 expression and cell adhesion in human monocytes. We observed that LacCer specifically exerted a time-dependent increase in PECAM-1 expression in U-937 cells. Maximal increase in PECAM-1 protein occurred after incubation with LacCer for 60 min. LacCer activated PKCalpha and -epsilon by translocating them from cytosol to membrane. This was accompanied by the activation of phospholipase A(2) (PLA(2)) and the increase of cell adhesion, which were abrogated by chelerythrine chloride, 2-[1-(3-dimethylaminopropyl)-1H-indol-3-yl]-3-(1H-indol-3-yl)-maleimide and 12-(2-cyanoethyl)-6,7,12,13-tetrahydro-13-methyl-5-oxo-5H-indolo(2,3-a)pyrrolo(3,4-c)-carbazole (GO 6976) (PKC inhibitors). Similarly, bromoenol lactone (a Ca(2+)-independent PLA(2) inhibitor) and methyl arachidonyl fluorophosphonate (an inhibitor of cytosolic PLA(2) and Ca(2+)-independent PLA(2)) inhibited LacCer-induced PLA(2) activity. Bromophenacyl bromide (a PLA(2) inhibitor) abrogated LacCer-induced PECAM-1 expression, and this was bypassed by arachidonic acid. Furthermore, the arachidonate-induced up-regulation of PECAM-1 was abrogated by indomethacin [a cyclooxygenase (COX)-1 and -2 inhibitor] or N-[2-(cyclohexyloxy)-4-nitrophenyl]-methanesulfonamide (a COX-2 inhibitor) but not nordihydroguaiaretic acid (a lipoxygenase inhibitor). In sum, PKCalpha/epsilon are the primary targets for the activation of LacCer. Downstream activation of intracellular Ca(2+)-independent PLA(2) and/or cytosolic PLA(2) results in the production of arachidonic acid, which in turn serves as a precursor for prostaglandins that subsequently stimulate PECAM-1 expression and cell adhesion. These findings may be relevant in explaining the role of LacCer in the regulation of PECAM-1 and related pathophysiology.
Design and application of SAE J1939 communication protocol in electric vehicle (2014)

Recently, cooperative control actions of electric vehicle become much more complex than before, it is important and significant to manage and allocate energy by a network in electric vehicle. According to the electric vehicle control requirements, this paper have designed a network architecture based on the vehicle CAN network communication protocol SAE1939, including high-speed network, slow-speed network and charging network, three different CAN network subnet; it have also designed the database of CAN network application layer, message format and time scheduling period, semaphores, etc. a new method for estimating the worst case delay and designed a simulation platform is been proposed to test the situation of CAN bus network under various conditions of the vehicle, and applied the database in the real electric vehicle, the result could meet the requirements.
DARCY CONVECTION ANISOTROPIC PROBLEM WITH MULTISTABILITY OF STATIONARY MOTIONS FOR RECTANGLE (2023)

Based on the Darcy model, uid convection in a porous rectangle is analyzed taking into account anisotropy of thermal characteristics and permeability. Relations between parameters for which the problem belongs to the class of cosymmetric systems are derived. For this case explicit formulas for the critical numbers of the loss of stability of mechanical equilibrium are found. Using a nite-di erence method that preserves the cosymmetry of the problem, family of stationary convective regimes is computed. Through the computational experiment the destruction of families is demonstrated in the case of violation of the conditions of cosymmetry. As result the appearance of a nite number of stationary regimes are obtained.
Pseudotrypsin: A Little-Known Trypsin Proteoform (2018)

Trypsin is the protease of choice for protein sample digestion in proteomics. The most typical active forms are the single-chain β-trypsin and the two-chain α-trypsin, which is produced by a limited autolysis of β-trypsin. An additional intra-chain split leads to pseudotrypsin (ψ-trypsin) with three chains interconnected by disulfide bonds, which can be isolated from the autolyzate by ion-exchange chromatography. Based on experimental data with artificial substrates, peptides, and protein standards, ψ-trypsin shows altered kinetic properties, thermodynamic stability and cleavage site preference (and partly also cleavage specificity) compared to the above-mentioned proteoforms. In our laboratory, we have analyzed the performance of bovine ψ-trypsin in the digestion of protein samples with a different complexity. It cleaves predominantly at the characteristic trypsin cleavage sites. However, in a comparison with common tryptic digestion, non-specific cleavages occur more frequently (mostly after the aromatic residues of Tyr and Phe) and more missed cleavages are generated. Because of the preferential cleavages after the basic residues and more developed side specificity, which is not expected to occur for the major trypsin forms (but may appear anyway because of their autolysis), ψ-trypsin produces valuable information, which is complementary in part to data based on a strictly specific trypsin digestion and thus can be unnoticed following common proteomics protocols.
Tolerance Induction by CD40 Blocking through Specific Antibody in Dendritic Cells. (2010)

Blocking antibodies are valuable tools for inhibiting the specific receptor- ligand interactions. The interaction of co-stimulatory molecules on the antigen presenting cells with their ligands on T cells is an essential step for T cell activation. In the present study, the effect of blocking antibody against CD40 on its T cell stimulatory potential is investigated.The DCs (dendritic cells) were collected from the mice spleens and then cultured in vitro. We used purified rat anti-mice CD40 (Clone HM40-3) (BD USA) as a blocking antibody and the appropriate titer of the blocking antibody was determined by flow cytometry. The DCs were then treated by antibody and used in MLR assay. The results of these experiments showed that CD40 blockade were associated with the increase in the of IL-4 secretion, shifting the DCs to stimulate Th2 cytokine production by the allogenic T cells, while the secretion of IL-12 by DCs decreased. Similarly, the DCs with reduced CD40 expression poorly responded to alloantigen stimulation in the MLR. Collectively, these results emphasize the importance of CD40 pathway in tolerogenic DCs generation and also support the idea that downregulation of CD40 is effective in inhibiting the allostimulatory function.