gebhart/patrepeval huggingface dataset:
Ontogeny of growth hormone (GH)-secreting cells during chicken embryonic development: initial somatotrophs are responsive to GH- releasing hormone (1995)

In the present study, a reverse hemolytic plaque assay (RHPA) for chicken GH was established and used to study the ontogeny of somatotroph differentiation and functional responsiveness to GH-releasing hormone (GHRH) during chicken embryonic development. Anterior pituitaries from embryos on days 10, 12, 14, and 16 of incubation were isolated and dissociated into single cells with trypsin. The resulting cells were then subjected to the GH plaque assay under basal and GHRH-stimulated conditions. No GH-releasing cells were detected on day 10 or 12 of embryonic development. In contrast, a few somatotrophs (< 2% of all cells) were consistently found on day 14, and a statistically significant population existed on day 16, when 6.3 +/- 1.4% of all anterior pituitary cells secreted GH. Thus, GH-secreting cells differentiated by embryonic day 16. Treatment of pituitary cells from day 16 embryos with GHRH was found to increase the proportion of GH plaque-forming cells during a shortened assay interval from 1.8 +/- 0.3% under basal conditions to 6.7 +/- 1.2% in the presence of GHRH. This nearly 4-fold increase in the proportion of plaque-forming cells indicates that at least 70% of the initial somatotrophs present on day 16 were responsive to the stimulatory effects of GHRH. To test whether the absence of GH cells on day 12 of embryonic development was due to the presence of cells that produced but did not release GH, pituitary cells from day 12 and day 16 embryos were subjected to immunocytochemistry for GH and to the GH RHPA in parallel. No significant differences were found in the percentage of cells that either contained or released GH on the two embryonic ages tested. On day 12, 1.1 +/- 0.8% of all cells contained GH, as determined by immunocytochemistry, whereas 0.5 +/- 0.5% released GH as determined by RHPA. By day 16, the proportions of cells that contained and released GH had increased to 9.5 +/- 0.6 and 11.2 +/- 2.5%, respectively. Taken together, these results indicate that GH-secreting cells differentiate by day 16 of chicken embryonic development and that these initial somatotrophs are responsive to GHRH. Given that growth and metabolism are regulated in part by GH in chick embryos, these findings suggest that these processes may be under hypothalamic control during late embryonic development in the chicken.
Young Children in Foster Care (2005)

Young children who have been removed from their biological families and placed in foster care are at significant risk for poor developmental outcomes. Their vulnerability is often the result of adverse biological and psychosocial influences: prenatal exposure to alcohol and other drugs, premature birth, abuse and neglect leading to foster placement, and failure to form adequate attachments to their primary caregivers. Children younger than 6 years form the largest group entering foster care, and remain longest in care. Meeting the complex needs of this vulnerable group of young children and their families presents extensive challenges for early intervention service systems. The purpose of the following discussion is to describe the foster care population and the kinds of medical conditions, mental health problems, and developmental disabilities experienced by young children in foster care, and to explore implications for intervention. By increasing their understanding of risk factors, vulnerabilities, and complex service needs, early childhood professionals can become effective advocates and provide services that ameliorate risk and optimize outcomes for these children and their families.
Realization of mobility-controlled flying router in information-centric networking (2018)

Constructing a large-scale network in outdoor fields with wired or wireless antennas involves a huge deployment cost. New devices, called movable routers, are able to realize the networks at a low cost. Recently, a large number of internet of things nodes have been deployed into the networks in outdoor fields. In the current communication network, users intend to communicate with devices without knowing where they are, but the current network still focuses on device locations, complicating configuration and implementation of the network. In this study, we design an architecture called router-movable information-centric networking (RMICN) to realize content-based communication instead of host-based communication, for facilitating communications between disjointed networks using movable routers. We incorporate information-centric networking technologies in the design of the system for the naming scheme and the strategy layer, enabling device control over the movable routers, called flying routers. In the simulation results, we show that RMICN can further reduce the response time of content requests compared with delay-tolerant networking. Furthermore, we present a proof-of-concept implementation of the architecture to confirm the feasibility of the design.
Expression and characterization of calmodulin-activated (type I) adenylylcyclase (1991)

A complementary DNA that encodes a bovine brain, calmodulin-sensitive (type I) adenylylcyclase has been inserted into the baculovirus genome under the control of the strong polyhedron promoter. Expression of the recombinant adenylylcyclase in Sf9 cells using recombinant baculovirus increases adenylylcyclase activity in cell membranes to 10-20 nmol.min-1.mg-1 (approximately 0.1% of membrane protein). The catalytic activity of the recombinant adenylylcyclase can be stimulated by Gs alpha, calmodulin, or forskolin, and it can be inhibited by adenosine analogs and by G protein beta gamma subunit. The specific activity of the purified recombinant protein approximates 5 mumol.min-1.mg-1. This is similar to that of the enzyme purified from bovine brain. Type I adenylylcyclase has a quasiduplicated structure. There are two membrane-spanning domains, each with six putative transmembrane helices, and there are two presumed nucleotide-binding domains that are about 55% similar to each other. No catalytic activity is detectable when each half of the adenylylcyclase molecule is expressed by itself. However, coexpression of the two halves results in considerable enzymatic activity. Interaction between the two halves of adenylylcyclase may be necessary for catalysis.
The librarian's assistant: automatically organizing on-line books into dynamic bookshelves (1994)

Constructing bookshelves is usually done manually by the providers of online-book collections. This task requires human expertise and becomes tedious even for experts as soon as books become too numerous. This paper addresses the problem of automatically constructing bookshelves. It presents a tool, the Librarian's Assistant, which makes use of numerical as well as conceptual clustering techniques to generate a hierarchy of bookshelves from a set of unorganized books. The Librarian's Assistant can be used as a stand alone tool to assist documentation producers in the process of building manual bookshelves, in order to understand the underlying structure of a given library, as well as a facility integrated in an IR or hypertext system to expand the scope of searches.
Indel-correcting DNA barcodes for high-throughput sequencing (2018)

Many large-scale, high-throughput experiments use DNA barcodes, short DNA sequences prepended to DNA libraries, for identification of individuals in pooled biomolecule populations. However, DNA synthesis and sequencing errors confound the correct interpretation of observed barcodes and can lead to significant data loss or spurious results. Widely used error-correcting codes borrowed from computer science (e.g., Hamming, Levenshtein codes) do not properly account for insertions and deletions (indels) in DNA barcodes, even though deletions are the most common type of synthesis error. Here, we present and experimentally validate filled/truncated right end edit (FREE) barcodes, which correct substitution, insertion, and deletion errors, even when these errors alter the barcode length. FREE barcodes are designed with experimental considerations in mind, including balanced guanine-cytosine (GC) content, minimal homopolymer runs, and reduced internal hairpin propensity. We generate and include lists of barcodes with different lengths and error correction levels that may be useful in diverse high-throughput applications, including >10<sup>6</sup> single-error-correcting 16-mers that strike a balance between decoding accuracy, barcode length, and library size. Moreover, concatenating two or more FREE codes into a single barcode increases the available barcode space combinatorially, generating lists with >10<sup>15</sup> error-correcting barcodes. The included software for creating barcode libraries and decoding sequenced barcodes is efficient and designed to be user-friendly for the general biology community.
Trace learning path by mobile technologies as an instructional tool using GPS and GIS in students'field trip to zoo (2006)

With the development of wireless technology, many handheld computers provide the learners with platform and resources to interact, collaborate, and share ubiquitously. The purpose of this research is to explore the potential application of mobile technologies in supporting elementary students' learning in the field trip. By recording the path of traversing, the GPS and GIS offer hyperlinked to the information on the Internet for further learning after the trip. The result indicates that the mobile technologies can be used to provide an adaptive learning support to the zoo visitors. Teachers could integrate teaching material well for further instruction.
Measuring Imputed Cost in the Semiconductor Equipment Supply Chain (2003)

We consider the order-fulfillment process of a supplier producing a customized capital good, such as production equipment, commercial aircraft, medical devices, or defense systems. As is common in these industries, prior to receiving a firm purchase order from the customer, the supplier receives a series of shared forecasts, which are called “soft orders.” Facing a stochastic internal manufacturing lead time, the supplier must decide at what time to begin the fulfillment of the order. This decision requires a trade-off between starting too early, leading to potential holding or cancellation costs, and starting too late, leading to potential delay costs. We collect detailed data of shared forecasts, actual purchase orders, production lead times, and delivery dates for a supplier-buyer dyad in the semiconductor equipment supply chain. Under the assumption that the supplier acts rationally, optimally balancing the cancellation, holding, and delay costs, we are able to estimate the corresponding imputed cost parameters based on the observed data. Our estimation results reveal that the supplier perceives the cost of cancellation to be about two times higher and the holding costs to be about three times higher than the delay cost. In other words, the supplier is very conservative when commencing the order fulfillment, which undermines the effectiveness of the overall forecast-sharing mechanism.
Packet-Level Network Compression: Realization and Scaling of the Network-Wide Benefits (2015)

The existence of considerable amount of redundancy in the Internet traffic at the packet level has stimulated the deployment of packet-level redundancy elimination techniques within the network by enabling network nodes to memorize data packets. Redundancy elimination results in traffic reduction which in turn improves the efficiency of network links. In this paper, the concept of network compression is introduced that aspires to exploit the statistical correlation beyond removing large duplicate strings from the flow to better suppress redundancy. In the first part of the paper, we introduce “memory-assisted compression,” which utilizes the memorized content within the network to learn the statistics of the information source generating the packets which can then be used toward reducing the length of codewords describing the packets emitted by the source. Using simulations on data gathered from real network traces, we show that memory-assisted compression can result in significant traffic reduction. In the second part of the paper, we study the scaling of the average network-wide benefits of memory-assisted compression. We discuss routing and memory placement problems in network for the reduction of overall traffic. We derive a closed-form expression for the scaling of the gain in Erdös-Rényi random network graphs, where obtain a threshold value for the number of memories deployed in a random graph beyond which network-wide benefits start to shine. Finally, the network-wide benefits are studied on Internet-like scale-free networks. We show that non-vanishing network compression gain is obtained even when only a tiny fraction of the total number of nodes in the network are memory-enabled.
Transcription of the Huntington disease gene during the quinolinic acid excitotoxic cascade (1995)

Although Huntington disease (HD) is characterized by the selective neurodegeneration of the basal ganglia and cerebral cortex, efforts to define the disease pathology have been complicated by the widespread expression of the disease gene (IT15) throughout the body. In this study, we examined IT15 mRNA levels during the quinolinic acid (QA) excitotoxic cascade to determine whether neuronal and/or glial expression is regulated by neurodegeneration. Following an initial increase between 1 h and 6 h, IT15 mRNA levels declined in a pattern homologous to a group of neuron-specific genes. Decreased mRNA levels after 24 h demonstrated that glial transcription is not activated by neurodegeneration or gliosis. The 1 h and 24 h mRNA levels strongly suggest that IT15 transcription preferentially localizes to degenerating neurons.
Modeling Support of Effects-Based Operations in War Games (2002)

Abstract : The Effects Based Operations (EBO) concept is based on relating actions in a battle plan to overall effects. A prototype system called CAESAR II/EB has been developed to assist in analyzing Courses of Action (COAs) for Effects-Based Operations and evaluating them in terms of the probability of achieving the desired effects. The tool supports both static and dynamic evaluation of COAs by integrating influence nets with discrete event systems modeling techniques. Preliminary operational concepts for using this tool in command and control environments were tested during the Naval War College Global 2000 and the Global 2001 war games providing insights into the appropriateness of these techniques in support of EBO. In Global 2001 the authors worked with different cells and components to produce four models of the complete battle plan to support the planning phase and six quick reaction models to support the execution phase of the game. The interaction of the modeling team with the multiple command and control cells in the game and the potential as a COA decision support was tested and examined. This paper describes the experiences with building and using the models and discusses requirements for enhancements to the modeling techniques generated from this experience.
Human eosinophil peroxidase: purification and characterization. (1985)

Human eosinophil peroxidase (EPO) was isolated from granules from granulocytes of a patient with hypereosinophilia. The granules were extracted by means of 0.2 M NaAc, pH 4.0. The purification steps included gel filtration chromatography on Sephadex G-75 superfine and ion-exchange chromatography on CM-Sephadex G-50. The purified protein showed one band on agarose-electrophoresis, a high peroxidase activity, and a 415-nm/280 nm ratio of 1.15. After reduction, EPO showed two bands on SDS-PAGE of m.w. 52,000 and 15,000, respectively. On gel filtration, the unreduced protein had a m.w. of approximately 77,000. Amino acid analyses showed a high content of arginine and aspartic acid. Monospecific antibodies to EPO were prepared in rabbits, and a specific radioimmunoassay was developed. There was an almost linear correlation between the content of EPO measured by the radioimmunoassay and the number of eosinophils in a mixed cell extract from reference material, indicating the eosinophil origin of EPO. The content of EPO was estimated to be 15.0 micrograms/10(6) eosinophils.
Annual Report of the Certification Officer 1987 (1988)

Annual Report of the Certification Officer 1987 Get access MARK HALL MARK HALL *University of Bradford Management Centre Search for other works by this author on: Oxford Academic Google Scholar Industrial Law Journal, Volume 17, Issue 1, 1988, Pages 203–205, https://doi.org/10.1093/ilj/17.1.203 Published: 01 June 1988
Hydraulic Fracturing 101: What Every Representative, Environmentalist, Regulator, Reporter, Investor, University Researcher, Neighbor and Engineer Should Know About Estimating Frac Risk and Improving Frac Performance in Unconventional Gas and Oil Wells. (2012)

Abstract Identification of risk, the potential for occurrence of an event and impact of that event, is the first step in improving a process by ranking risk elements and controlling potential harm from occurrence of a detrimental event. Hydraulic Fracturing has become a hot environmental discussion topic and a target of media articles and University studies during development of gas shales near populated areas. The furor over fracturing and frac waste disposal was largely driven by lack of chemical disclosure and the pre-2008 laws of some states. The spectacular increase in North American natural gas reserves created by shale gas development makes shale gas a disruptive technology, threatening profitability and continued development of other energy sources. Introduction of such a disruptive force as shale gas will invariably draw resistance, both monetary and political, to attack the disruptive source, or its enabler; hydraulic fracturing. Some "anti-frack" charges in media articles and university studies are based in fact and require a state-by-state focused improvement of well design specific for geology of the area and oversight of overall well development. Other articles have demonstrated either a severe misunderstanding or an intentional misstatement of well development processes, apparently to attack the disruptive source. Transparency requires cooperation from all sides in the debate. To enable more transparency on the oil and gas side, both to assist in the understanding of oil and gas activities and to set a foundation for rational discussion of fracturing risks, a detailed explanation of well development activities is offered in this paper, from well construction to production, written at a level of general public understanding, along with an initial estimation of frac risk and alternatives to reduce the risk, documented by literature and case histories. This discussion is a starting point for the well development descriptions and risk evaluation discussions, not an ending point.
Synthesis of Carbon Nanoparticles via Co-Pyrolysis of Waste Slop Oil and Ferrocene (2015)

Co-pyrolysis of slop oil with ferrocene was carried out to convert waste of petroleum into carbon nanoparticles (CNPs). Since slop oil is a mixture of hydrocarbons (HCs) with broad molecular weight distribution, it could be simply fractionated into some certain fractions by batch distillation. Distillate containing hydrocarbons with small molecules was mainly focused as an alternative carbon source for synthesis of CNPs. A two-stage furnace was employed for evaporating a mixture of distillated slop oil and ferrocene at 200 °C in the 1 st stage of the furnace and then formation of CNPs at 900 °C could be observed in the 2 nd stage. Laboratory-grade ferrocene was mixed with slop oil with a designated weight-ratio of 1:2. Microscopic analyses based on SEM and TEM micrographs reveals that CNPs obtained from distillated slop oil mostly consist of bundles of multi-walled carbon nanotubes (MWCNTs) with nominal diameters of 20-50 nm. Raman spectroscopic analyses of the synthesized CNPs exhibit the notably high value of I G / I D , suggesting that the synthesized CNPs preferably consist of graphitic nanostructure. Moreover, TGA analysis shows that 39.8 and 32.9 wt% of Fe contents exist in the CNP samples synthesized from original slop oil and distillated slop oil, respectively.
Amitraz: a tick and flea repellent and tick detachment drug (1986)

A topical formulation of amitraz (Mitaban Liquid Concentrate, The Upjohn Company, Kalamazoo, Michigan, U.S.A.) was evaluated as a tick repellent and detachment agent, and flea repellent. The diluted liquid concentrate (250 p.p.m. active drug) was topically applied as a single treatment to dogs; the concentration was identical to the rate recommended for treatment of demodicosis and scabies. Brown dog tick (Rhipicephalus sanguineus) and American dog tick (Dermacentor variabilis) populations were eliminated and repelled. Repellent activity (92-95%) was observed against R. sanguineus for 2 weeks post-treatment; the treatment was moderately active (63%) during the third week, and at 4 weeks post-treatment the drug was inactive. Established R. sanguineus populations were also treated, and the diluted liquid concentrate had 100% tick-detachment efficacy. Repellent activity (99%) was also detected against D. variabilis; the activity was monitored for only 7 days. The ectoparasiticide had low to moderate flea (Ctenocephalides felis) repellent activity (42%) for 4 days post-treatment; thereafter the treatment was ineffective. Side-effects were not observed in any of the dogs treated with amitraz, or the placebo.
Tunable coupling scheme for implementing two-qubit gates on fluxonium qubits (2021)

The superconducting fluxonium circuit is an RF-SQUID-type flux qubit that uses a large inductance built from an array of Josephson junctions or a high kinetic inductance material. This inductance suppresses charge sensitivity exponentially and flux sensitivity quadratically. In contrast to the transmon qubit, the anharmonicity of fluxonium can be large and positive, allowing for better separation between the low energy qubit manifold of the circuit and higher-lying excited states. Here, we propose a tunable coupling scheme for implementing two-qubit gates on fixed-frequency fluxonium qubits, biased at half flux quantum. In this system, both qubits and coupler are coupled capacitively and implemented as fluxonium circuits with an additional harmonic mode. We investigate the performance of the scheme by simulating a universal two-qubit fSim gate. In the proposed approach, we rely on a planar on-chip architecture for the whole device. Our design is compatible with existing hardware for transmon-based devices, with the additional advantage of lower qubit frequency facilitating high-precision gating.
Rapid infection in market-weight swine following exposure to a Salmonella Typhimurium-contaminated environment (2001)

Pigs can become infected during routine resting or holding periods during marketing when exposed to relatively low amounts of Salmonella organisms in the preslaughter environment. Intervention at this step of the production process may have a major impact on the safety of pork products.