gebhart/patrepeval huggingface dataset:
The need for dynamic models of drug markets1 (2001)

Markets for cocaine and heroin are created out of epidemics and are best analysed in terms of dynamic models rather than comparative statics. The author presents the following three problems requiring dynamic models and sketches a potential approach to each one: (a) why prices have declined so persistently in the face of intensified enforcement; (b) informing the choice between buyer and seller targeting for enforcement; and (c) explaining the extremely high variability of retail prices in such markets. For the first two problems, critical issues are the modelling of the path of sources of earnings (such as other crime or legitimate activities) and the drug-selling labour supply as consumption becomes dominated by drug users with increasingly long criminal histories and fewer legitimate employment opportunities. For the third problem, models need to take into account both the difficulty of ascertaining quality at the time of retail purchase and the instability of use and sale opportunities within those markets.
Improving the Repeatability and Efficacy of Intradermal Electroporated Self-Replicating mRNA (2019)

Local administration of naked self-replicating mRNA (sr-mRNA) in the skin or muscle using electroporation is effective but hampered by low repeatability. In this manuscript, we demonstrated that intradermal electroporation of sr-mRNA in combination with a protein-based RNase inhibitor increased the expression efficiency, success rate, and repeatability of the data. The RNase inhibitor should be added just before administration because storage of the inhibitor together with the sr-mRNA at -80°C resulted in a partial loss of the beneficial effect. Furthermore, the location of intradermal electroporation also had a major effect on the expression of the sr-mRNA, with the highest and longest expression observed at the tail base of the mice. In contrast with previous work, we did not observe a beneficial effect of calcium ions on the efficacy of naked sr-mRNA after intradermal injection. Finally, another important finding was that the traditional representation of in vivo bioluminescence data as means in logarithmic graphs can mask highly variable data. A more truthful representation can be obtained by showing the individual data points or by displaying median values in combination with interquartile ranges. In conclusion, intradermal sr-mRNA electroporation can be improved by adding an RNase inhibitor and injecting at the tail base.
Usefulness of Anti-CD117 in the Flow Cytometric Analysis of Acute Leukemia (2002)

We assessed the diagnostic usefulness of adding anti-CD117 to our existing flow cytometric profile in the analysis of 150 consecutive cases of acute leukemia (de novo or relapsed acute myelogenous leukemia [AML], AML arising in myelodysplastic syndrome, blast crisis of chronic myelogenous leukemia [CML], acute lymphoblastic leukemia, acute unclassifiable leukemia, and biphenotypic leukemia). CD117 was expressed on more than 10% of blasts in 64% of de novo AMLs (42/66), 95% of relapsed AMLs (19/20), 75% of AMLs arising from a myelodysplastic syndrome (6/8), and 25% of myeloid blast crisis in CMLs (1/4). CD117 was not expressed in acute lymphoblastic, acute biphenotypic, or unclassified leukemia or lymphoid blast crisis of CML. The specificity, positive predictive value, sensitivity, and negative predictive value of CD117 for AML were 100%, 100%, 69%, and 62%, respectively. CD117 is a specific marker for myeloblastic leukemias. Sensitivity is greatest in French-American-British M2 and relapsed AML. Intensity of CD117 expression is dim. Despite the high specificity and positive predictive value, the addition of anti-CD117 to our panel did not prove essential for the assignment of blast lineage.
A Signature-based Grid Index Design for RFID Main-Memory Databases (2008)

A large-scaled RFID application often needs a highly efficient database system in data processing. This research is motivated by the strong demand of an efficient index structure design for main-memory database systems of RFID applications. In this paper, a signature-based grid index structure is proposed for efficient data queries and storage. An efficient methodology is proposed to locate duplicates and to execute batch deletions and range queries based on application domain knowhow. The capability of the design is implemented in an open source main-memory database system H2 and evaluated by realistic workloads of RFID applications.
Acquisition Behavior of a First-Order Digital Phase-Locked Loop (1978)

A general "phase-plane" technique for investigating the stability of nonlinear difference equation is described and applied to determine the stability criterion of a first-order digital phase-locked loop (DPLL). If the loop is stable, it is shown that acquisition behavior can be modeled as a first passage time problem. Using this model, one can evaluate the acquisition probability and the mean time to acquire, from the appropriate Chapman-Kolmogorov (C-K) equation describing the transition of the phase error sequence. This approach is verified by direct simulation.
Energy-Free Adjustment of Gravity Equilibrators Using the Virtual Spring Concept (2007)

People with neuromuscular diseases often have limited muscle force, and many rely on mobile arm supports to move their arms. Most of these make use of static balancing, a useful concept to reduce the operating effort of mechanisms, where spring mechanisms are used to achieve a constant total potential energy, thus eliminating any preferred position. Once statically balanced, the mechanism can be moved virtually without operating energy. This allows the patients using an arm support to move their arms freely. In some cases it is desirable to adjust the balancer characteristic, for instance due to a change of payload when picking up an object. In all available arm support mechanisms this adjustment is associated with considerable mechanical effort, while clearly this application would benefit greatly from an energy-free adjustment. Recently several methods have been invented to adjust spring and linkage-based static balancers with no need for external energy, by conserving the total energy in the system. The technique discussed in this paper is based on substituting an initial spring by two substitute springs. The two substitute springs generate a virtual spring with the same spring properties as the initial spring, with one unique difference: the virtual spring can be elongated or shortened while no work is done. Consequently, no external energy is needed for the adjustment. A table-top demonstrator has been developed, showing the feasibility of the concept.
Experiences of use cases and similar concepts (1992)

article Experiences of use cases and similar concepts Share on Author: Fredrik Lindström View Profile Authors Info & Claims ACM SIGPLAN OOPS MessengerVolume 4Issue 2April 1993 pp 123–130https://doi.org/10.1145/157710.157732Online:01 December 1992Publication History 1citation560DownloadsMetricsTotal Citations1Total Downloads560Last 12 Months1Last 6 weeks0 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteGet Access
Synergistic Targeting of Cell Membrane, Cytoplasm, and Nucleus of Cancer Cells Using Rod-Shaped Nanoparticles (2013)

Design of carriers for effective delivery and targeting of drugs to cellular and subcellular compartments is an unmet need in medicine. Here, we report pure drug nanoparticles comprising camptothecin (CPT), trastuzumab (TTZ), and doxorubicin (DOX) to enable cell-specific interactions, subcellular accumulation, and growth inhibition of breast cancer cells. CPT is formulated in the form of nanorods which are coated with TTZ. DOX is encapsulated in the TTZ corona around the CPT nanoparticle. Our results show that TTZ/DOX-coated CPT nanorods exhibit cell-specific internalization in BT-474 breast cancer cells, after which TTZ is recycled to the plasma membrane, leaving CPT nanorods in the perinuclear region and delivering DOX into the nucleus of the cells. The effects of CPT-TTZ-DOX nanoparticles on growth inhibition are synergistic (combination index = 0.17 ± 0.03) showing 10-10 000-fold lower inhibitory concentrations (IC50) compared to those of individual drugs. The design of antibody-targeted pure drug nanoparticles offers a promising design strategy to facilitate intracellular delivery and therapeutic efficiency of anticancer drugs.
Code Space Enlargement for Hybrid Fiber Radio and Baseband OCDMA PONs (2011)

A hybrid analog and digital passive optical network based on optical code division multiple access is presented, and a code generation method is proposed to increase the number of codes for existing code families with fixed and ideal in-phase cross correlations. When code families with cyclic properties are used by this code generation method, compact encoders based on arrayed waveguide gratings can be used. The corresponding performance for this hybrid network is also analyzed.
Bridging computer vision and social science: A multi-camera vision system for social interaction training analysis (2015)

We investigate the use of a vision-based system capable of estimating social states such as rapport and hostility. We study the correlation between interpretations automatically generated by our system and those reported by social scientists. Our multi-camera vision system collects visual cues including location (proximity), motion, pose, gaze, and facial expressions in real-time from multiple subjects moving freely in an unconstrained environment. We performed experiments on 80+ subjects. Preliminary regression analysis suggests high correlation between machine distilled time series signals and assessments made by human experts.
Do anxiety and depression have a common pathophysiological mechanism? (2000)

The proposed hypothesis remains to be tested by examination of either the changes in receptors and neurotransmission or the mechanisms underlying the dysregulation of endocrine factors.
Stromal 5α-reductase activity is elevated in benign prostatic hyperplasia (1980)

The activities of 5 alpha-reductase and 3 alpha (beta)-hydroxysteroid dehydrogenase were assayed in homogenates of stroma and epithelium obtained from 3 normal, 9 hyperplastic and 2 carcinomatous human prostates. Irrespective of the normal or abnormal condition of the prostate, the localization of 5 alpha-reductase was predominantly in stroma whereas the reductive and oxidative activities of 3 alpha (beta)-hydroxysteroid dehydrogenase were more evenly divided between stroma and epithelium. Furthermore, the mean specific activity of 5 alpha-reductase in hyperplastic stroma at 84.6 plus or minus 13.1 (plus or minus SEM) pmol 30 min-1 mg protein-1 was almost 3 times greater (Student's t-test, P < 0.05) than the corresponding value in normal stroma, 31.6 plus or minus 7.2 pmol mg protein-1 30 min-1. We conclude that the stroma is the primary site of conversion of testosterone to dihydrotestosterone in the human prostate owing to the prevalence in stroma of 5 alpha-reductase, and that benign prostatic hyperplasia is characterized by an increased amount of stromal 5 alpha-reductase activity.
Feasibility of a Neutron Detector-dosemeter based on Single-event Upsets in Dynamic Random-access Memories (2002)

The feasibility was investigated of a solid-state neutron detector/dosemeter based on single-event upset (SEU) effects in dynamic random-access memories (DRAMs), commonly used in computer memories. Such a device, which uses a neutron converter material to produce a charged particle capable of causing an upset, would be light-weight, low-power, and could be read simply by polling the memory for bit flips. It would have significant advantages over standard solid-state neutron dosemeters which require off-line processing for track etching and analysis. Previous efforts at developing an SEU neutron detector/dosemeter have suffered from poor response, which can be greatly enhanced by selecting a modern high-density DRAM chip for SEU sensitivity and by using a thin 10B film as a converter. Past attempts to use 10B were not successful because the average alpha particle energy was insufficient to penetrate to the sensitive region of the memory. This can be overcome by removing the surface passivation layer before depositing the 10B film or by implanting 10B directly into the chip. Previous experimental data show a 10(3) increase in neutron sensitivity by chips containing borosilicate glass, which could be used in an SEU detector. The results are presented of simulations showing that the absolute efficiency of an SEU neutron dosemeter can be increased by at least a factor of 1000 over earlier designs.
A Highly Modular Cloning Vector for the Analysis of Eukaryotic Genes and Gene Regulatory Elements (1985)

We have developed a highly modular vector, pDSP1, which contains two independent mammalian transcription cassettes. Each cassette contains SV40 early gene regulatory elements controlling the expression of an easily assayable, selectable Escherichia coli marker gene, either galK or xgprt. The regulatory elements of the galK cassette are bounded by multiple unique and nearly unique restriction sites allowing for the easy removal and replacement of either the regulatory sequences or of the galK gene itself. Expression of the marker genes is monitored by transient transfection into mammalian cells followed by filter enzyme assays. Expression of xgprt serves as an internal control and the relative expression of galK/xgprt is used to quantitate modifications made to the vector. We have used this system to analyze many eukaryotic polyadenylation regions as well as several other eukaryotic gene regulatory elements. We have also removed the galK gene and replaced it with other mammalian genes. The entire galK cassette is contained on a Sal I restriction fragment that can be readily removed and placed into a unique Sal I site in one of our Epstein-Barr virus (EBV), bovine papilloma virus (BPV), or BK defective viral stable expression vectors. We believe that pDSP1 is a powerful vector system for studying eukaryotic gene regulation, and in conjunction with our stable expression vectors, it represents a unified system for exploring expression in mammalian cells both transiently and stably.
Expression of Sea Anemone Equistatin in Potato. Effects of Plant Proteases on Heterologous Protein Production (2003)

Abstract Plants are increasingly used as production platforms of various heterologous proteins, but rapid protein turnover can seriously limit the steady-state expression level. Little is known about specific plant proteases involved in this process. In an attempt to obtain potato (Solanum tuberosum cv Desirée) plants resistant to Colorado potato beetle (Leptinotarsa decemlineata Say) larvae, the protease inhibitor equistatin was expressed under the control of strong, light-inducible and constitutive promoters and was targeted to the secretory pathway with and without endoplasmic reticulum retention signal. All constructs yielded similar stepwise protein degradation patterns, which considerably reduced the amount of active inhibitor in planta and resulted in insufficient levels for resistance against Colorado potato beetle larvae. Affinity purification of the degradation products and N-terminal sequencing allowed the identification of the amino acid P1-positions (asparagine [Asn]-13, lysine-56, Asn-82, and arginine-151) that were cleaved in planta. The proteases involved in the equistatin degradation were characterized with synthetic substrates and inhibitors. Kininogen domain 3 completely inhibited equistatin degradation in vitro. The results indicate that arginine/lysine-specific and legumain-type Asn-specific cysteine proteases seriously impede the functional accumulation of recombinant equistatin in planta. General strategies to improve the resistance to proteases of heterologous proteins in plants are proposed.
Efficient and Provable Multi-Query Optimization (2017)

Complex queries for massive data analysis jobs have become increasingly commonplace. Many such queries contain common subexpressions, either within a single query or among multiple queries submitted as a batch. Conventional query optimizers do not exploit these subexpressions and produce sub-optimal plans. The problem of multi-query optimization (MQO) is to generate an optimal combined evaluation plan by computing common subexpressions once and reusing them. Exhaustive algorithms for MQO explore an O(nn) search space. Thus, this problem has primarily been tackled using various heuristic algorithms, without providing any theoretical guarantees on the quality of their solution.
LDL-cholesterol: a risk factor for coronary artery disease--from epidemiology to clinical trials. (1998)

Discussion of potential strategies to modify lipids and lipoproteins other than low density lipoproteins (LDLs) should first recognize the convincing evidence in favour of the identification and aggressive treatment of elevated LDL cholesterol (LDL-C) levels in patients with established cardiovascular disease. Elevated LDL-C level is one of the few risk factors for which there is evidence of involvement in every pathophysiological step of the development of cardiovascular disease. Longitudinal studies have established the role of LDL-C as a risk factor for cardiovascular disease incidence, recurrence and fatal outcome. Clinical trials and economic analyses have proven that aggressive treatment of elevated LDL-C in patients at high risk can prevent cardiac events with excellent cost effectiveness.
Thermal evolution of H-related defects in H implanted Si: from nano-platelets to micro-cracks (2018)

Annealing at moderate temperatures (300-450°C) of H implanted silicon leads to the formation of nano-platelets within minutes and of micro-cracks or blisters within hours. Up to now, the mechanism by which they transform from one to another type of H <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> filled defect was unknown. We have analyzed the evolution of the characteristics of platelets populations at the onset of micro-cracks formation and found it occurs when and where a group of "large" platelets of 50 nm in diameter are close enough, typically at about 50 nm from each other. In situ TEM confirms that sudden nucleation of micro-cracks in place of groups of platelets occurs. It is concluded that micro-crack nucleation results from elastic interactions between neighboring platelets, favoring the opening of the silicon crystal between them.
Division accuracy in a stochastic model of Min oscillations in <i>Escherichia coli</i> (2005)

Accurate cell division in Escherichia coli requires the Min proteins MinC, MinD, and MinE as well as the presence of nucleoids. MinD and MinE exhibit spatial oscillations, moving from pole to pole of the bacterium, resulting in an average MinD concentration that is low at the center of the cell and high at the poles. This concentration minimum is thought to signal the site of cell division. Deterministic models of the Min oscillations reproduce many observed features of the system, including the concentration minimum of MinD. However, there are only a few thousand Min proteins in a bacterium, so stochastic effects are likely to play an important role. Here, we show that Monte Carlo simulations with a large number of proteins agree well with the results from a deterministic treatment of the equations. The location of minimum local MinD concentration is too variable to account for cell division accuracy in wild-type, but is consistent with the accuracy of cell division in cells without nucleoids. This finding confirms the need to include additional mechanisms, such as reciprocal interactions with the cell division ring or positioning of the nucleoids, to explain wild-type accuracy.
<i>Arabidopsis</i> NPH3: A NPH1 Photoreceptor-Interacting Protein Essential for Phototropism (1999)

Phototropism of Arabidopsis thaliana seedlings in response to a blue light source is initiated by nonphototropic hypocotyl 1 (NPH1), a light-activated serine-threonine protein kinase. Mutations in three loci [NPH2, root phototropism 2 (RPT2), and NPH3] disrupt early signaling occurring downstream of the NPH1 photoreceptor. The NPH3 gene, now cloned, encodes a NPH1-interacting protein. NPH3 is a member of a large protein family, apparently specific to higher plants, and may function as an adapter or scaffold protein to bring together the enzymatic components of a NPH1-activated phosphorelay.
Efficacy, Safety, and Plasma Pharmacokinetics of Escalating Dosages of Intravenously Administered Ravuconazole Lysine Phosphoester for Treatment of Experimental Pulmonary Aspergillosis in Persistently Neutropenic Rabbits (2004)

Ravuconazole is a new antifungal triazole with broad-spectrum activity and a long half-life in plasma. We studied the antifungal efficacy, safety, and pharmacokinetics of ravuconazole lysine phosphoester in escalating dosages for the treatment of invasive pulmonary aspergillosis due to Aspergillus fumigatus in persistently neutropenic rabbits. Treatment groups consisted of rabbits treated with ravuconazole at 2.5 (RVC2.5), 5 (RVC5), and 10 (RVC10) mg/kg of body weight/day, rabbits treated with amphotericin B (AMB) at 1 mg/kg/day, or untreated controls. There was a dose-dependent reduction of pulmonary residual fungal burden (CFU per gram) in RVC5-, RVC10-, and AMB-treated rabbits in comparison to untreated controls (P < 0.01, P < 0.001, and P < 0.01, respectively). These findings correlated with progressive galactomannan antigenemia in untreated controls and the RVC2.5-treated rabbits, a lower galactomannan index (GMI) in RVC5- and RVC10-treated rabbits, and a similarly low GMI in AMB-treated rabbits (P < 0.01). Rabbits treated with RVC5, RVC10, and AMB also showed a reduction of organism-mediated pulmonary injury, as measured by infarct scores and lung weights, in comparison to untreated controls (P < 0.001). These results were supported by decreased pulmonary infiltrates detected by computed tomography in RVC5- and RVC10-treated rabbits in comparison to untreated controls (P < 0.05). Survival throughout the entire study was achieved in 95% of RVC5-treated rabbits (P < 0.001), 85% of RVC10-treated rabbits (P < 0.001), and 50% of AMB-treated rabbits (P < 0.05) in comparison to none of the untreated controls. Ravuconazole showed linear plasma pharmacokinetics and a large volume of distribution while maintaining concentrations in plasma above the MIC throughout the dosing interval. There was no evidence of hepatotoxicity or nephrotoxicity among ravuconazole-treated animals. Intravenously administered ravuconazole lysine phosphoester showed dose-dependent efficacy and an excellent safety profile for the treatment of invasive pulmonary aspergillosis in persistently neutropenic rabbits.