gebhart/patrepeval huggingface dataset:
In situ forming hydrogels of new amino hyaluronic acid/benzoyl-cysteine derivatives as potential scaffolds for cartilage regeneration (2012)

A new chemical strategy is described to link ethylenediamino (EDA) groups to primary hydroxyl groups of hyaluronic acid (HA) and the obtained derivatives have been characterized by 1H-NMR and 13C-NMR analyses. Such HA–EDA derivatives have been exploited to control the functionalization degree in benzoyl-cysteine (BC) groups, chosen as moieties able to allow both self-assembling in aqueous media and an oxidative crosslinking. In particular, the kinetics of oxidation of thiol groups in HA–EDA–BC derivatives has been studied in Dulbecco's Phosphate Buffer Solution (DPBS) pH 7.4 by colorimetric assays and rheological measurements. Mechanical properties of chemical hydrogels obtained after oxidative crosslinking have been evaluated by using two different concentration values (1 and 1.5% w/v) of HA–EDA–BC. These hydrogels show a dense interconnected fibrillar structure similar to the extracellular matrix of soft tissue and a resistance to chemical and enzymatic hydrolysis. Therefore, the potential suitability of HA–EDA–BC hydrogels as scaffolds for cartilage regeneration has been preliminarily assessed using primary human chondrocytes and evaluating their viability and ability to produce extracellular collagen.
Synthesis and properties of polycyanoethylmethylsiloxane polyurea urethane elastomers: A study of segmental compatibility (1988)

Abstract A series of polyurea urethane block polymers based on either aminopropyl‐terminated polycyanoethylmethylsiloxane (PCEMS) soft segments or soft segment blends of PCEMS and polytetramethylene oxide (PTMO) were synthesized. The hard segments consisted of 4,4′‐methylenediphenylene diisocyanate (MDI) chain‐extended with 1,4‐butanediol. The hard segment content varied from 11 to 36%, whereas the PTMO weight fraction in the soft segment blends varied from 0.1 to 0.9. The cyanoethyl side group concentration was also varied during the synthesis of the PCEMS oligomer. The morphology and properties of these polymers were studied by differential scanning calorimetry, infrared spectroscopy, dynamic mechanical and tensile testing, and small‐angle x‐ray scattering. These materials exhibited microphase separation of the hard and soft segments; however, attaching polar cyanoethyl side groups along the apolar siloxane chains promoted phase mixing in comparison with polydimethylsiloxane‐based polyurethanes. The increased phase mixing is postulated to lead to improved interfacial adhesion and thus can account for the observed improvement in ultimate tensile properties compared with polydimethylsiloxane‐based polyurethanes. Both hard segment content and cyanoethyl concentration are important factors governing the morphological and tensile properties of these polymers.
Indoor Positioning Based on LTE Carrier Phase Measurements and an Inertial Measurement Unit (2018)

A tightly-coupled inertial navigation system (INS) with cellular long-term evolution (LTE) signal aiding for indoor positioning is developed. The navigation approach is based on a carrier phase-based software-defined receiver (SDR), which tracks LTE signals and provides aiding corrections to an inertial measurement unit (IMU) in a tightly-coupled fashion via an extended Kalman filter (EKF). The indoor positioning framework employs an outdoor receiver, referred to as the base, which estimates the unknown clock biases of LTE eNodeBs and shares these estimates with the indoor navigating receiver. The availability and strength of received LTE signals indoors is evaluated in different conditions: different floor levels and in rooms with and without windows. It is demonstrated that the received carrier-to-noise ratio C/N0 in all such conditions is 48-80 dB-Hz. The performance of the developed system is evaluated in an indoor environment over a trajectory of 109 m using a tactical-grade IMU. The two-dimensional position root mean-squared error (RMSE) for the proposed LTE-IMU system is demonstrated to be 3.52 m with a standard deviation of 3.85 m and a maximum error of 8.1 m. In contrast, the IMU-only system provided an RMSE of 9.91 m with a standard deviation of 9.67 m and a maximum error of 22.53 m.
An external cavity diode laser sensor (1983)

A simple compact optical sensor system consisting of a near-field external cavity coupled to a semiconductor laser is reported. In this device, a change of phase in the light fed back into the laser cavity by the perturbed external mirror modulates the output of the laser by effectively changing the laser facet reflectivity. Phase shifts of <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">9\cdot10^{-8}</tex> rad and 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-6</sup> rad were measured at 10 kHz and 100 Hz, respectively, using a 1-Hz bandwidth and a 98-percent reflector placed less than 10 μm from the laser facet. The sensitivity of these devices is shown to be limited by the intrinsic amplitude noise of the laser. The implementation of this sensor configuration is reported as an acoustic sensor, hydrophone, magnetic field, and current sensor as well as an accelerometer.
Lanthanum nickel aluminum alloy. [Patent application; LaNi/sub 5-x/Al/sub x/, x = 0. 01 to 1. 5] (1977)

A tertiary intermetallic compound capable of reversible sorption of hydrogen having the chemical formula LaNi/sub 5-x/Al/sub x/, where x is in the range of about 0.01 to 1.5, and the method of storing hydrogen using the intermetallic compound are described. 1 figure.
Expression of the Platencin Biosynthetic Gene Cluster in Heterologous Hosts Yielding New Platencin Congeners (2012)

Platensimycin (PTM) and platencin (PTN) are potent and selective inhibitors of bacterial and mammalian fatty acid synthases and have emerged as promising drug leads for both antibacterial and antidiabetic therapies. We have previously cloned and sequenced the PTM-PTN dual biosynthetic gene cluster from Streptomyces platensis MA7327 and the PTN biosynthetic gene cluster from S. platensis MA7339, the latter of which is composed of 31 genes encoding PTN biosynthesis, regulation, and resistance. We have also demonstrated that PTM or PTN production can be significantly improved upon inactivation of the pathway-specific regulator ptmR1 or ptnR1 in S. platensis MA7327 or MA7339, respectively. We now report engineered production of PTN and congeners in a heterologous Streptomyces host. Expression constructs containing the ptn biosynthetic gene cluster were engineered from SuperCos 1 library clones and introduced into five model Streptomyces hosts, and PTN production was achieved in Streptomyces lividans K4-114. Inactivation of ptnR1 was crucial for expression of the ptn biosynthetic gene cluster, thereby PTN production, in S. lividans K4-114. Six PTN congeners, five of which were new, were also isolated from the recombinant strain S. lividans SB12606, revealing new insights into PTN biosynthesis. Production of PTN in a model Streptomyces host provides new opportunities to apply combinatorial biosynthetic strategies to the PTN biosynthetic machinery for structural diversity.
Probiotics: From Strain to Product (2010)

This chapter contains sections titled: Introduction Isolating a potential probiotic strain Producing probiotic strains on a large scale Producing products containing probiotics Probiotic products and feeding trials Conclusion
Micropattern platform promotes extracellular matrix remodeling by human PSC‐derived cardiac fibroblasts and enhances contractility of co‐cultured cardiomyocytes (2021)

In native heart tissue, cardiac fibroblasts provide the structural framework of extracellular matrix (ECM) while also influencing the electrical and mechanical properties of cardiomyocytes. Recent advances in the field of stem cell differentiation have led to the availability of human pluripotent stem cell-derived cardiac fibroblasts (iPSC-CFs) in addition to cardiomyocytes (iPSC-CMs). Here we use a novel 2D in vitro micropatterned platform that provides control over ECM geometry and substrate stiffness. When cultured alone on soft micropatterned substrates, iPSC-CFs are confined to the micropatterned features and remodel the ECM into anisotropic fibers. Similar remodeling and ECM production occurs when cultured with iPSC-CMs in a co-culture model. In addition to modifications in the ECM, our results show that iPSC-CFs influence iPSC-CM function with accelerated Ca<sup>2+</sup> transient rise-up time and greater contractile strains in the co-culture conditions compared to when iPSC-CMs are cultured alone. These combined observations highlight the important role cardiac fibroblasts play in vivo and the need for co-culture models like the one presented here to provide more representative in vitro cardiac constructs.
Structural Requirements for Agonist Activity of a Murine Interferon-γ Peptide (1996)

We have demonstrated previously that murine interferon-gamma (MuIFN-gamma) binds to the extracellular domain of the receptor alpha chain through its N-terminus and subsequently to the cytoplasmic domain of the receptor via its C-terminus. Binding of the C-terminus to the cytoplasmic domain of the receptor is thought to occur following endocytosis of the IFN-gamma-receptor complex. In fact, the MuIFN-gamma C-terminus peptide, MuIFN-gamma (95-133), has full agonist activity on macrophages where it is internalized through pinocytosis. Here we examine the structural elements required for the agonist activity of MuIFN-gamma (95-133). Disruption of the alpha helical structure of the peptide by proline substitutions or truncation of the helix resulted in significant loss of binding or loss of antiviral activity or both and induction of MHC class II molecules. Further, removal of the polycationic sequence RKRKR in the tail beyond the helical structure also resulted in loss of agonist activity. Thus, we have isolated the functional site on MuIFN-gamma to the C-terminus and have shown that its helical structure and polycationic tail are required for binding to the cytoplasmic domain of the receptor and induction of biologic activity.
Identification of Different Binding Sites in the Dendritic Cell-specific Receptor DC-SIGN for Intercellular Adhesion Molecule 3 and HIV-1 (2002)

The novel dendritic cell (DC)-specific human immunodeficiency virus type 1 (HIV-1) receptor DC-SIGN plays a key role in the dissemination of HIV-1 by DC. DC-SIGN is thought to capture HIV-1 at mucosal sites of entry, facilitating transport to lymphoid tissues, where DC-SIGN efficiently transmits HIV-1 to T cells. DC-SIGN is also important in the initiation of immune responses by regulating DC-T cell interactions through intercellular adhesion molecule 3 (ICAM-3). We have characterized the mechanism of ligand binding by DC-SIGN and identified the crucial amino acids involved in this process. Strikingly, the HIV-1 gp120 binding site in DC-SIGN is different from that of ICAM-3, consistent with the observation that glycosylation of gp120, in contrast to ICAM-3, is not crucial to the interaction with DC-SIGN. A specific mutation in DC-SIGN abrogated ICAM-3 binding, whereas the HIV-1 gp120 interaction was unaffected. This DC-SIGN mutant captured HIV-1 and infected T cells in trans as efficiently as wild-type DC-SIGN, demonstrating that ICAM-3 binding is not necessary for HIV-1 transmission. This study provides a basis for the design of drugs that inhibit or alter interactions of DC-SIGN with gp120 but not with ICAM-3 or vice versa and that have a therapeutic value in immunological diseases and/or HIV-1 infections.
Pharmacological characterization of four related substance P antagonist (1982)

The purpose of this pharmacological study was to further characterize 4 related substance P (SP) analogues, namely (D‐Pro 2 , D‐Phe 7 , D‐Trp 9 )‐SP (I), (D‐Pro 2 , D‐Trp 7.9 )‐SP (II), (D‐Arg 1 , D‐Pro 2 , D‐Phe 7 , D‐Trp 9 )‐SP (III) and (D‐Arg 1 , D‐Pro 2 , D‐Trp 7.9 )‐SP (IV). At a concentration of 10 ‐4 M they were found to have little or no smooth muscle‐contracting effect on the guinea‐pig ileum, analogues I and II having substantially less agonist activity than III and IV. Pretreatment with the analogues inhibited the contractile responses to SP, but not to histamine or acetylcholine. Of the 4 analogues, II was found to be the most potent SP antagonist. The contractile responses to physalaemin and eledoisin were also inhibited by analogue II. The concentration‐response curves for SP were shifted in parallel to higher concentrations in the presence of the analogues. The pA 2 ‐values derived from Schild plots were 4.61 for (I), 5.43 for (II), 4.69 for (III) and 5.11 for (IV). Except for (I) the slopes of the regression lines of the Schild plots were close to unity. The data are consistent with simple competitive antagonism over the concentration ranges investigated. Without being secretagogues per se, the analogues inhibited SP‐stimulated salivary secretion in the rat. Physalaemin‐stimulated secretion was inhibited by analogue II. The inhibitory effect of sequences with D‐Arg 1 instead of L‐Arg 1 seemed to be of longer duration. Not one of these analogues blocked the blood pressure‐lowering effect of SP, which indicates the existence of more than one type of SP receptor. This study has shown that 4 related SP analogues specifically inhibit the actions of SP and structurally related peptides physalaemin and eledoisin, both in vitro and in vivo. Analogues II‐IV seem to inhibit in a simple competitive manner. (D‐Pro 2 , D‐Trp 7.9 )‐SP was the most potent of the 4 SP antagonists.
Functional Analysis of Rice DREB1/CBF-type Transcription Factors Involved in Cold-responsive Gene Expression in Transgenic Rice (2005)

The transcription factors dehydration-responsive element-binding protein 1s (DREB1s)/C-repeat-binding factors (CBFs) specifically interact with the DRE/CRT cis-acting element and control the expression of many stress-inducible genes in Arabidopsis. The genes for DREB1 orthologs, OsDREB1A and OsDREB1B from rice, are induced by cold stress, and overexpression of DREB1 or OsDREB1 induced strong expression of stress-responsive genes in transgenic Arabidopsis plants, resulting in increased tolerance to high-salt and freezing stresses. In this study, we generated transgenic rice plants overexpressing the OsDREB1 or DREB1 genes. These transgenic rice plants showed not only growth retardation under normal growth conditions but also improved tolerance to drought, high-salt and low-temperature stresses like the transgenic Arabidopsis plants overexpressing OsDREB1 or DREB1. We also detected elevated contents of osmoprotectants such as free proline and various soluble sugars in the transgenic rice as in the transgenic Arabidopsis plants. We identified target stress-inducible genes of OsDREB1A in the transgenic rice using microarray and RNA gel blot analyses. These genes encode proteins that are thought to function in stress tolerance in the plants. These results indicate that the DREB1/CBF cold-responsive pathway is conserved in rice and the DREB1-type genes are quite useful for improvement of stress tolerance to environmental stresses in various kinds of transgenic plants including rice.
<title>A fast MAP-based super-resolution algorithm for general motion</title> (2006)

We propose a fast MAP-based super-resolution algorithm for reconstructing a high-resolution image (HRI) by combining multiple low-resolution images (LRIs). The proposed algorithm optimizes a cost function with respect to the HRI in the frequency domain, whereas existing MAP algorithms optimize with respect to the HRI in the spatial domain. Calculation amount comparison verifies that the proposed algorithm has a much smaller calculation cost than a classical algorithm. Experiments using real images are also demonstrated. They show that the proposed algorithm greatly hastens the super-resolution process, reconstructing an identical HRI to the classical algorithm.
The Staphylococcus aureus Map protein is an immunomodulator that interferes with T cell–mediated responses (2002)

Staphylococcus aureus (SA) is an opportunistic pathogen that affects a variety of organ systems and is responsible for many diseases worldwide. SA express an MHC class II analog protein (Map), which may potentiate SA survival by modulating host immunity. We tested this hypothesis in mice by generating Map-deficient SA (Map–SA) and comparing disease outcome to wild-type Map+SA–infected mice. Map–SA–infected mice presented with significantly reduced levels of arthritis, osteomyelitis, and abscess formation compared with control animals. Furthermore, Map–SA–infected nude mice developed arthritis and osteomyelitis to a severity similar to Map+SA–infected controls, suggesting that T cells can affect disease outcome following SA infection and Map may attenuate cellular immunity against SA. The capacity of Map to alter T cell function was tested more specifically in vitro and in vivo using native and recombinant forms of Map. T cells or mice treated with recombinant Map had reduced T cell proliferative responses and a significantly reduced delayed-type hypersensitivity response to challenge antigen, respectively. These data suggest a role for Map as an immunomodulatory protein that may play a role in persistent SA infections by affecting protective cellular immunity.
Checkpoints-on-demand with active replication (2002)

Checkpointing and roll-back recovery is a well known technique for recovering from software process failures. Analytical models have been developed for computing the completion time of processes that use various checkpointing strategies such as periodic checkpointing, random checkpointing etc. In this paper, we show that with active replication of processes, a strategy that uses a mechanism we call checkpoints-on-demand will result in an expected completion time smaller than that can be achieved with traditional schemes that use periodic checkpoints. With checkpoints-on-demand, when a process fails, it is recovered from an induced checkpoint taken of a replica of the process. Recovery of persistent server processes through state-transfer from a replica has been proposed in the context of group communication systems and in the process cloning approach of the Delta-4 architecture. But it has not been previously proposed and analyzed as a mechanism for reducing the expected completion time of a long running process.
ChemInform Abstract: Organic—Inorganic Hybrid Lead Halide Perovskites for Optoelectronic and Electronic Applications (2016)

Abstract Review: 374 refs.
Vehicular Path Prediction for Cooperative Driving Applications through Digital Map and Dynamic Vehicle Model Fusion (2009)

This paper investigates the fusion of digital maps and dynamical models to accurately predict the future path of a vehicle. This path prediction is generated by a vehicle for sharing with other vehicles through wireless communication channels. This sharing is an enabler of Vehicle-to-Vehicle (V2V) cooperative systems. In particular, this work looks at cooperative safety and comfort systems, which benefit from accurate path predictions for collision avoidance and coordination with other vehicles. This work proposes that digital map information is valuable in giving a nominal estimate of the future path of the vehicle, and that this estimate can be augmented with realtime vehicle information, such as wheel speeds, yaw rates, and accelerations. Furthermore, dynamic short-term situations such as lane changes can be cleanly incorporated into the vehicular path prediction. Depending on the driving environment, the sensor quality, the map accuracy, and the map matching precision, this paper shows that this fusion has the potential to provide where-in-lane path predictions.
Single-Photon Source for Quantum Information Based on Single Dye Molecule Fluorescence in Liquid Crystal Host (2006)

Abstract This paper describes a new application for liquid crystals: quantum information technology. A deterministically polarized single-photon source that efficiently produces photons exhibiting antibunching is a pivotal hardware element in absolutely secure quantum communication. Planar-aligned nematic liquid crystal hosts deterministically align the single dye molecules which produce deterministically polarized single (antibunched) photons. In addition, 1-D photonic bandgap cholesteric liquid crystals will increase single-photon source efficiency. The experiments and challenges in the observation of deterministically polarized fluorescence from single dye molecules in planar-aligned glassy nematic-liquid-crystal oligomer as well as photon antibunching in glassy cholesteric oligomer are described for the first time. Keywords: antibunchingliquid crystalssingle-dye moleculessingle-photon source Acknowledgments The authors acknowledge the support by the U.S. Army Research Office under Award No. DAAD19-02-1-0285 and National Science Foundation Awards ECS-0420888, EEC-0243779, PHY-0242483. The work was also supported by the U.S. Department of Energy Office of Inertial Confinement Fusion under Cooperative Agreement No. DE-FC03-92SF19460, the University of Rochester, and the New York State Energy Research and Development Authority. The support of DOE does not constitute an endorsement by DOE of the views expressed in this article. Receipt of LC oligomers from S.-H. Chen, Cornerstone Research Group, Inc. and F. Kreuzer of Wacker, Munich are gratefully acknowledged. The authors thank L. Novotny, A. Lieb, J. Shojaie, A. Trajkovska, S. Culligan, for advice and help, and J. Starowitz for support in optical material laboratory.
A Fully Encapsulated Directional Self-Powered Gamma Ray Detector for Use in in-Core Nuclear Reactor Measurements (1980)

A study of a fully encapsulated directional self-powered gamma ray detector designed for localized incore measurements in a nuclear reactor was conducted. The detector consisted of a multilayer arrangement of a metal-dielectric-metal-dielectric-metal structure. The dielectric material was made of two plates of unequal thicknesses which were placed on opposite sides of the central metal plate. The direction discrimination exhibited by the detector was attributed to the combined effect of electron ranges, Photo-Compton electron generation rates, and the presence of E-fields in the unequal thicknesses of dielectric material. Results showing the response of the detector when it was placed in a gamma ray field with a known anisotropic component are presented.
Temperature dependent leakage power characteristics of dynamic circuits in sub-65 nm CMOS technologies (2005)

The temperature dependent subthreshold and gate oxide leakage power characteristics of domino logic circuits are evaluated in this paper. The preferred input vectors and node voltage states that minimize the total leakage power consumption are identified at the lower and upper extremes of a typical die temperature spectrum. New low leakage circuit design guidelines are presented based on the results. The significantly increased gate dielectric tunneling current, as described in this paper, dramatically changes the leakage power characteristics of dynamic circuits in the sub-65nm CMOS technologies. Furthermore, the leakage power savings provided by the dual threshold voltage domino logic circuit techniques are all together reduced due to the significance of the gate dielectric tunneling in the nanometer CMOS technologies.