gebhart/patrepeval huggingface dataset:
Inhibition of cellular differentiation by the SCL/tal oncoprotein: transcriptional repression by an Id-like mechanism (1995)

In cases of T-cell acute lymphoblastic leukemia (T-ALL), the basic helix-loop-helix (bHLH) oncogene SCL/tal undergoes frequent rearrangements activating ectopic expression. Despite the compelling epidemiological association of SCL/tal expression with T-ALL, no specific transforming function has been attributable to the protein product. However, investigators have recently demonstrated that forced overexpression of SCL/tal can block monocytic differentiation of M1 murine myeloid leukemia cells. Thus, inappropriate expression of wild-type SCL/tal protein may in part account for the maturation arrest phenotype observed in T-ALL cells. In this study, ectopic expression of the SCL/tal gene blocked the differentiation of C2C12 muscle precursor cells. Characterization of the mechanism of differentiation blockade showed that the SCL/tal protein repressed transcriptional activation by the myogenic bHLH factor MyoD. Protein interaction analysis showed that SCL/tal and MyoD compete for common partners (E bHLH proteins) but do not directly bind one other. A model is thus proposed in which ectopic SCL/tal protein, by its ability to titrate out E proteins, prevents the formation of bHLH complexes that drive cellular differentiation: the "Id-like" mechanism.
Effect of Urokinase on Corneal Endothelium (1980)

Urokinase in concentrations ranging from 1,000 to 5,000 units/mL was used to perfuse the corneal endothelium of rabbits for three hours in the specular microscope. No notable corneal swelling was noted, and the corneal endothelium appeared normal when observed with scanning electron microscopy.
Aggregation of the hairy rod conjugated polyelectrolyte poly{1,4-phenylene-[9,9-bis(4-phenoxybutylsulfonate)]fluorene-2,7-diyl} in aqueous solution: an experimental and molecular modelling study (2008)

The aggregation of the fluorescent hairy rod, anionic conjugated polyelectrolyte poly{1,4-phenylene-[9,9-bis(4-phenoxybutylsulfonate)]fluorene-2,7-diyl} (PBS-PFP) has been studied in aqueous solutions by molecular dynamics simulations, fluorescence and light scattering. Formation of clusters leads to considerable increases in light scattering, decreases in the fluorescence quantum yields and red shifts in emission maxima. Molecular dynamics simulations considering two isolated tetramers in aqueous solution show that they rapidly form aggregates, and support experimental evidence for the association of polymer chains involving both electrostatic and hydrophobic interactions. They also provide indications for proximity of aromatic rings, which is likely to be the main factor responsible for the observed fluorescence behaviour. However, there are no indications of extensive pi-stacking. The organic co-solvents methanol, acetonitrile and dioxane break up these aggregates. From studies of the dependence of the aggregation behaviour on dielectric constant or the empirical solvent parameters E(N)(T) and B(KT) for binary mixtures with water, it can be seen that this is not simply an effect of changing solvent polarity, but is due to preferential solvation of the polymer chains. This is supported by molecular dynamic simulations on two tetramers in water-dioxane mixtures (70:30%). It is suggested that similar factors are involved in both the association behaviour and aggregate disruption with other hairy rod conjugated polyelectrolytes in water.
Contribution of chromatic aberrations to color signals in the primate visual system (2006)

We measured responses to red-green color variation in parvocellular (PC) neurons in the lateral geniculate nucleus of dichromatic ("red-green color blind") marmoset monkeys. Although these animals lack distinct visual pigments to distinguish between wavelengths in this range, many of the colored stimuli nevertheless produced robust responses in PC cells. We show that these responses, which are restricted to high stimulus spatial frequencies (fine image details), arise from chromatic aberrations in the eye. The neural signals produced by chromatic aberrations are of comparable magnitude to signals produced by high-frequency luminance (LUM) modulation and thus could influence cortical pathways for processing of color and object recognition. The fact that genetically "color-blind" primates are not necessarily blind to wavelength-dependent contours in the visual world may have enabled red-green color vision to become linked with high-acuity spatial vision during primate evolution.
Aryl Mesylates in Metal Catalyzed Homo- and Cross-Coupling Reactions. 4. Scope and Limitations of Aryl Mesylates in Nickel Catalyzed Cross-Coupling Reactions (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAryl Mesylates in Metal Catalyzed Homo- and Cross-Coupling Reactions. 4. Scope and Limitations of Aryl Mesylates in Nickel Catalyzed Cross-Coupling ReactionsVirgil Percec, Jin-Young Bae, and Dale H. HillCite this: J. Org. Chem. 1995, 60, 21, 6895–6903Publication Date (Print):October 1, 1995Publication History Published online1 May 2002Published inissue 1 October 1995https://pubs.acs.org/doi/10.1021/jo00126a047https://doi.org/10.1021/jo00126a047research-articleACS PublicationsRequest reuse permissionsArticle Views2250Altmetric-Citations218LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Photonic band gap fiber accelerator (2001)

There are three requirements in making a traveling wave accelerator: longitudinal electric field, synchronization, and confinement. We present and analyze a conceptually new kind of charged particle accelerator, making use of a photonic band gap lattice for field confinement near the beam axis, and employing dielectric material to produce a speed of light synchronous longitudinal electric field. An example structure design is presented. We also discuss nonlinear effect and other configurations without higher order dipole modes. Parallel scheme and fabrication are presented.
A selective and sensitive approach to characterize odour‐active and volatile constituents in small‐scale human milk samples (2007)

Abstract A sensitive and selective analytical approach was developed for the characterization of trace volatile and odorous substances in body fluids. The methodology was successfully applied for identification of more than 40 characteristic odorants in human milk. The technique comprises a modified stir bar sorptive extraction system in combination with two‐dimensional gas chromatographic separation and parallel mass spectrometric and olfactometric characterization of the analytes. The present study shows that the technique can be used for both direct extractive sampling and headspace analysis, and that it is applicable for small sample volumes. Copyright © 2007 John Wiley & Sons, Ltd.
Actin regulation in endocytosis (2006)

Increasing evidence from a variety of cell types has highlighted the importance of the actin cytoskeleton during endocytosis. No longer is actin viewed as a passive barrier that must be removed to allow endocytosis to proceed. Rather, actin structures are dynamically organised to assist the remodelling of the cell surface to allow inward movement of vesicles. The majority of our mechanistic insight into the role of actin in endocytosis has come from studies in budding yeast. Although endocytosis in mammalian cells is clearly more complex and subject to a greater array of regulatory signals, recent advances have revealed actin, and actin-regulatory proteins, to be present at endocytic sites. Furthermore, live cell imaging indicates that spatiotemporal aspects of actin recruitment and vesicle formation are likely to be conserved across eukaryotic evolution.
Interpolated finite impulse response filters (1984)

A new approach to implement computationally efficient finite impulse response (FIR) digital filters is presented. The filter structure is a cascade of two sections. The first section generates a sparse set of impulse response samples and the other section generates the remaining samples by using interpolation. The method can be used to implement most practical FIR filters with significant savings in the number of arithmetic operations. Typically 1/2 to 1/8 of the number of multipliers and adders of conventional FIR filters are required in the implementation. The saving is achieved both in the linear phase and the non-linear phase cases. In addition, the new implementation gives smaller coefficient sensitivities and better roundoff noise properties than conventional implementations.
DRP : distribution resource planning : the gateway to true quick response and continuous replenishment (1995)

Total Marketing Channel Integration: The Last Frontier. Quick Response/Continuous Replenishment: New Thinking. Typical Problems in Logistics: Symptoms and Causes. The DRP Management Process: The ABCs of DRP. DRP in Nonmanufacturing Environments: Retailers, Wholesalers, and Distributors. DRP in a Manufacturing Environment: Using DRP with Sales and Operations Planning and Master Production Scheduling. Tools of the Trade: Getting the Most of a DRP System. Forecasting: The DRP Connection. Solving Logistics Problems: Common Approaches. Transportation Planning: Taking Advantage of Cost-Saving Opportunities. Financial Planning and Budgeting Simulation: Beyond Traditional Accounting. Management Issues: Problems on the Road to Successful DRP. Appendices. Index.
HCMV glycoprotein B subunit vaccine efficacy mediated by nonneutralizing antibody effector functions (2018)

Human cytomegalovirus (HCMV) is the most common congenital infection worldwide, frequently causing hearing loss and brain damage in afflicted infants. A vaccine to prevent maternal acquisition of HCMV during pregnancy is necessary to reduce the incidence of infant disease. The glycoprotein B (gB) + MF59 adjuvant subunit vaccine platform is the most successful HCMV vaccine tested to date, demonstrating ∼50% efficacy in preventing HCMV acquisition in multiple phase 2 trials. However, the mechanism of vaccine protection remains unknown. Plasma from 33 postpartum women gB/MF59 vaccinees at peak immunogenicity was tested for gB epitope specificity as well as neutralizing and nonneutralizing anti-HCMV effector functions and compared with an HCMV-seropositive cohort. gB/MF59 vaccination elicited IgG responses with gB-binding magnitude and avidity comparable to natural infection. Additionally, IgG subclass distribution was similar with predominant IgG1 and IgG3 responses induced by gB vaccination and HCMV infection. However, vaccine-elicited antibodies exhibited limited neutralization of the autologous virus, negligible neutralization of multiple heterologous strains, and limited binding responses against gB structural motifs targeted by neutralizing antibodies including AD-1, AD-2, and domain I. Vaccinees had high-magnitude IgG responses against AD-3 linear epitopes, demonstrating immunodominance against this nonneutralizing, cytosolic region. Finally, vaccine-elicited IgG robustly bound membrane-associated gB on the surface of transfected or HCMV-infected cells and mediated virion phagocytosis, although were poor mediators of NK cell activation. Altogether, these data suggest that nonneutralizing antibody functions, including virion phagocytosis, likely played a role in the observed 50% vaccine-mediated protection against HCMV acquisition.
HUMAN-DATA INTERACTION IN LARGE AND HIGH-DIMENSIONAL DATA (2018)

Human-Data Interaction (HDI) is an emerging field which studies how humans make sense of large and complex data. Visual analytics tools are a central component of this sensemaking process. However, the growth of big data has affected their performance, resulting in latency in interactivity or long query-response times, both of which degrade one's ability to do knowledge discovery. To address these challenges, a new paradigm of data exploration has appeared in which a rapid but inaccurate result is followed by a succession of gradually more accurate answers. As the primary objective of this thesis, we investigated how this incremental latency affects the quantity and quality of knowledge discovery in an HDI system. We have developed a big data visualization tool and studied 40 participants in a think-aloud experiment, using this tool to explore a large and high-dimensional data. Our findings indicate that although incremental latency reduces the rate of discovery generation, it does not affect one's chance of making a discovery per each generated visualization, and it does not affect the correctness of those discoveries. However, in the presence of latency, utilizing contextual layers such as a map result in fewer mistakes while exploring higher-dimensional visualizations lead to more incorrect discoveries. As the secondary objective, we investigated what strategies improved a subject's performance. Our observations suggest that successful participants explore the data methodically, by first examining simple and familiar concepts and then gradually adding complexity to the visualizations, until they build a correct mental model of the inner workings of the tool. With this model, they generate several discovery patterns, each acting as a blueprint for forming new insights. Ultimately, some participants combined their discovery patterns to create multifaceted data-driven stories. Based on these observations, we propose design guidelines for developing HDI platforms for large and high-dimensional data.
Design And Simulation Of Low Speed Axial Flux Permanent Magnet (Afpm) Machine (2012)

In this paper presented initial design of Low Speed Axial Flux Permanent Magnet (AFPM) Machine with Non-Slotted TORUS topology type by use of certain algorithm (Appendix). Validation of design algorithm studied by means of selected data of an initial prototype machine. Analytically design calculation carried out by means of design algorithm and obtained results compared with results of Finite Element Method (FEM).
Adjuvants Enhancing Cross-Presentation by Dendritic Cells: The Key to More Effective Vaccines? (2018)

Over the last decades, vaccine development has advanced significantly in pursuing higher safety with less side effects. However, this is often accompanied by a reduction in vaccine immunogenicity and an increased dependency on adjuvants to enhance vaccine potency. Especially for diseases like cancer, it is important that therapeutic vaccines contain adjuvants that promote strong T cell responses. An important mode of action for such adjuvants is to prolong antigen exposure to dendritic cells (DCs) and to induce their maturation. These mature DCs are extremely effective in the activation of antigen-specific T cells, which is a pre-requisite for induction of potent and long-lasting cellular immunity. For the activation of CD8<sup>+</sup> cytotoxic T cell responses, however, the exogenous vaccine antigens need to gain access to the endogenous MHCI presentation pathway of DCs, a process referred to as antigen cross-presentation. In this review, we will focus on recent insights in clinically relevant vaccine adjuvants that impact DC cross-presentation efficiency, including aluminum-based nanoparticles, saponin-based adjuvants, and Toll-like receptor ligands. Furthermore, we will discuss the importance of adjuvant combinations and highlight new developments in cancer vaccines. Understanding the mode of action of adjuvants in general and on antigen cross-presentation in DCs in particular will be important for the design of novel adjuvants as part of vaccines able to induce strong cellular immunity.
Synthesis and structures of 5-(pyridyl)tetrazole complexes of Mn(ii) (2005)

New Mn(II) complexes containing 5-(2-pyridyl)tetrazole, 5-(3-cyano-4-pyridyl)tetrazole or 5-(4-pyridyl)tetrazole ligands are described. The complexes are prepared by reaction of the corresponding cyanopyridines with sodium azide in the presence of Mn(II) salts. All the complexes have been characterized by X-ray crystallography, which reveals that 5-(pyridyl)tetrazole ligands can coordinate to Mn through either type of nitrogen atom in the tetrazole residue or via the pyridyl group. In the solid state, extended 2D and 3D structures are produced through networks of hydrogen bonding (involving water molecules and the tetrazolate residue). Acidification of the complexes produces the corresponding free 5-(pyridyl)-1H-tetrazole.
Key-Value Memory Networks for Directly Reading Documents (2016)

Directly reading documents and being able to answer questions from them is an unsolved challenge. To avoid its inherent difficulty, question answering (QA) has been directed towards using Knowledge Bases (KBs) instead, which has proven effective. Unfortunately KBs often suffer from being too restrictive, as the schema cannot support certain types of answers, and too sparse, e.g. Wikipedia contains much more information than Freebase. In this work we introduce a new method, Key-Value Memory Networks, that makes reading documents more viable by utilizing different encodings in the addressing and output stages of the memory read operation. To compare using KBs, information extraction or Wikipedia documents directly in a single framework we construct an analysis tool, WIKIMOVIES, a QA dataset that contains raw text alongside a preprocessed KB, in the domain of movies. Our method reduces the gap between all three settings. It also achieves state-of-the-art results on the existing WIKIQA benchmark.
Intraarterial anti-leptin therapy via ICA protects ipsilateral CA1 neurons subjected to ischemia and reperfusion (2022)

Inhibition of leptin activity in hemispheric IR injury preserved the viability of ipsilateral hippocampal CA1 neurons, likely by preventing apoptosis and local inflammation. These results indicate that intraarterial anti-leptin therapy may have clinical potential in reducing hemispheric brain IR injury.
Fas/APO-1/CD95 system as a mediator of granulosa cell apoptosis in ovarian follicle atresia. (1996)

Current studies have shown that atresia of ovarian follicles is induced through apoptosis in granulosa cells. Several articles have been devoted to study of the molecular mechanisms responsible for APO-1/CD95 (Fas) is a cell surface protein that can mediate apoptosis in lymphoid cells, and Fas ligand was recently identified in a cytotoxic T cell line. To clarify the involvement of the Fas-Fas ligand system in granulosa cell apoptosis, we investigated the expression of Fas and Fas ligand at an individual cell level. For this purpose, we raised specific polyclonal antibodies against Fas and Fas ligand. Western blotting confirmed that our anti-Fas antibodies (anti-P2 and anti-P4) detect a specific band with a mol wt of 45 kDa in the lysate of ovaries from immature PMSG-treated rats or adult cyclic rats. In immature PMSG-treated rats, immunohistochemical analysis with these antibodies revealed specific staining of granulosa cells in secondary and tertiary follicles at an early stage of atresia, but not in healthy follicles. Fas messenger RNA was also found in granulosa cells of early atretic follicles using in situ hybridization. On the other hand, the anti-Fas ligand antibody (anti-P5) detected a specific 31-kDa band on a Western blot of the oocytes lysate, and the staining with the serum was localized to oocytes in most of developing follicles. Colocalization of Fas and Fas ligand in certain follicles intimately correlated with granulosa cell apoptosis, which was revealed by terminal deoxynucleotidyl transferase-mediated deoxy-UTP-biotin nick end labeling staining of DNA strand breaks. Finally, we found that interferon-gamma increased Fas expression on granulosa cells in vitro. Coculturing interferon-gamma-pretreated granulosa cells with zona-free oocytes induced granulosa cell apoptosis, which was confirmed by Hoechst 33342 dye staining and terminal deoxynucleotidyl transferase-mediated deoxy-UTP-biotin nick end labeling, and the killing effect of oocytes was abolished by the addition of anti-P2, which was expected to interrupt the interaction between Fas and Fas ligand. These results demonstrate that activation between Fas and Fas ligand. These results demonstrate that activation of the Fas-Fas ligand system is capable of initiating apoptosis in the ovary, as are a number of other stimuli, outside the immune system.
Advanced A/V Encryption Technique for Digital Rights Management (2006)

In this paper, we propose a new encryption method for copyright protection of MP3 and MPEG-4 coder. For the MP3 audio, the magnitude and phase information of modified discrete cosine transform (MDCT) coefficients is encrypted. DCT coefficients and motion vectors (MVs) are used for the encryption of the MPEG-4 video. Experimental results indicate that the proposed joint and partial encryption technique provides levels of security and achieves a simple and coding-efficient architecture with no adverse impact on error resilience
MRGPRX4 is a G protein-coupled receptor activated by bile acids that may contribute to cholestatic pruritus (2019)

Patients suffering from cholestasis, the slowing or stoppage of bile flow, commonly report experiencing an intense, chronic itch. Numerous pruritogens are up-regulated in cholestatic patient sera, including bile acids (BAs). Acute injection of BAs results in itch in both mice and humans, and BA-modulating therapy is effective in controlling patient itch. Here, we present evidence that human sensory neuron-expressed Mas-related G protein-coupled receptor X4 (MRGPRX4), an orphan member of the <i>Mrgpr</i> family of GPCRs, is a BA receptor. Using Ca<sup>2+</sup> imaging, we determined that pathophysiologically relevant levels of numerous BAs activated MRGPRX4. No mouse Mrgpr orthologs were activated by BAs. To assess the in vivo relevance of BA activation of MRGPRX4, we generated a humanized mouse with targeted expression of MRGPRX4 in itch-encoding sensory neurons. BAs activated MRGPRX4<sup>+</sup> sensory neurons at higher levels compared with WT neurons. Compared with control animals, MRGPRX4<sup>+</sup> mice scratched more upon acute injection of BAs and in a model of cholestatic itch. Overall, these data suggest that targeting MRGPRX4 is a promising strategy for alleviating cholestatic itch.
Photoreactivation in humans. (1993)

Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans the biological, physical, and social sciences.