gebhart/patrepeval huggingface dataset:
Simultaneous online estimation of junction temperature and current of IGBTs using emitter-auxiliary emitter parasitic inductance (2014)

A novel method is presented for online estimation of the junction temperature (Tj) of semiconductor chips in IGBT modules, based on the voltage drop (VEE') across the parasitic inductor that exists between the main emitter (E) and auxiliary emitter (E) terminals. The peak amplitude of the voltage drop (VEE') was found to depend on the junction temperature at a known current and DC link voltage. Also, the collector current can be estimated simultaneously, by integrating VEE' without the use of any additional sensors. Measurement circuits were implemented to estimate Tj and the current, and their results are discussed. The results of these measurement circuits when implemented in a real power electronic (PE) converter to estimate Tj and current in real time are also presented. This method opens up a full set of new opportunities for engineers and designers to better understand the behavior and performance of high power modules in real PE applications.
A direct signaling system for flexible access and deployment of telecommunication services (1997)

In current telecommunication networks, the value-added services available to subscribers are almost exclusively offered by the carrier providing local telephony service. This results from two factors: the processing for services is either based in or triggered from software executing on the access telecommunication switches, and signaling links over which subscribers request services are terminated on these access switches. In a direct signaling system, the signaling link from a subscriber does not necessarily terminate on an access switch, but rather on an intelligent server we call a call server. The call server invokes service functions and coordinates their interactions. This direct signaling system may be overlaid on various types of access networks, including circuit-switched telephony, packet telephony, wireless local loop, cable, etc. We discuss the design, implementation, and performance of a direct signaling system, including procedures that provide varying amounts of integration with current telecommunication systems. Our results show that a direct signaling system using a low-delay direct signaling link and in which no changes are made to existing telecommunicatione switches incurs approximately 100 ms higher postdial delay than a standard ISDN system. A direct signaling system in which telecommunication switches provide open interfaces and coordinate processing with direct signaling servers incurs approximately 100 ms lower postdial delay than a standard ISDN system.
An Endoscopic Technique for Restoration of Voice after Laryngectomy (1980)

Reports of restoration of voice after total laryngectomy include diversion of exhaled pulmonary air though planned or spontaneous fistulae with a variety of modified tracheal cannulas and valves. Limitations of these techniques include aspiration, scar closure of the shunts, wound complications, and failure to achieve voice consistently. We report a two-year experience with an endoscopic method using a unique valved prosthesis eliminating complicated surgical reconstructions, aspiration, and stenosis. Fifty-four of 60 patients (90%) achieved fluent voices with one deglutition problem. Radiation therapy preceded voice restoration in 63% of the patients and radical neck dissection in 72%. The endoscopic procedure, hospitalization and period of speech therapy are short and constitute a cost-effective voice rehabilitation program. The results of this simple method and lack of complications are encouraging.
An Emerging Role for Metabolomics in Nutrition Science (2013)

Nutritional research is undergoing a remarkable transformation driven by new technological tools. Because of the complexity of the components present in food and how they may interact with the biochemical networks of living organisms, nutrition cannot be considered a reductionist discipline. More holistic approaches, which are capable of gathering comprehensive, high-throughput amounts of data, appear to best enhance our understanding of the role of food in health and disease. In this context, global metabolite analysis, or 'metabolomics', is becoming an appealing research tool for nutrigenomics and nutrigenetics scientists. The purpose of the present review is to highlight some potential applications of metabolomics in nutrition research.
Laissez-Faire (2015)

Backscatter provides dual-benefits of energy harvesting and low-power communication, making it attractive to a broad class of wireless sensors. But the design of a protocol that enables extremely power-efficient radios for harvesting-based sensors as well as high-rate data transfer for data-rich sensors presents a conundrum. In this paper, we present a new <i>fully asymmetric</i> backscatter communication protocol where nodes blindly transmit data as and when they sense. This model enables fully flexible node designs, from extraordinarily power-efficient backscatter radios that consume barely a few micro-watts to high-throughput radios that can stream at hundreds of Kbps while consuming a paltry tens of micro-watts. The challenge, however, lies in decoding concurrent streams at the reader, which we achieve using a novel combination of time-domain separation of interleaved signal edges, and phase-domain separation of colliding transmissions. We provide an implementation of our protocol, LF-Backscatter, and show that it can achieve an order of magnitude or more improvement in throughput, latency and power over state-of-art alternatives.
Simultaneous measurement of lipid and aqueous layers of tear film using optical coherence tomography and statistical decision theory (2014)

The prevalence of Dry Eye Disease (DED) in the USA is approximately 40 million in aging adults with about $3.8 billion economic burden. However, a comprehensive understanding of tear film dynamics, which is the prerequisite to advance the management of DED, is yet to be realized. To extend our understanding of tear film dynamics, we investigate the simultaneous estimation of the lipid and aqueous layers thicknesses with the combination of optical coherence tomography (OCT) and statistical decision theory. In specific, we develop a mathematical model for Fourier-domain OCT where we take into account the different statistical processes associated with the imaging chain. We formulate the first-order and second-order statistical quantities of the output of the OCT system, which can generate some simulated OCT spectra. A tear film model, which includes a lipid and aqueous layer on top of a rough corneal surface, is the object being imaged. Then we further implement a Maximum-likelihood (ML) estimator to interpret the simulated OCT data to estimate the thicknesses of both layers of the tear film. Results show that an axial resolution of 1 μm allows estimates down to nanometers scale. We use the root mean square error of the estimates as a metric to evaluate the system parameters, such as the tradeoff between the imaging speed and the precision of estimation. This framework further provides the theoretical basics to optimize the imaging setup for a specific thickness estimation task.
MicroRNA In Vitro Diagnostics Using Immunoassay Analyzers (2015)

Our miRNA immunoassay allowed the measurement of miRNA signatures with sufficient analytical sensitivity and high specificity on commonly available laboratory equipment.
Action Segmentation with Mixed Temporal Domain Adaptation (2020)

The main progress for action segmentation comes from densely-annotated data for fully-supervised learning. Since manual annotation for frame-level actions is time-consuming and challenging, we propose to exploit auxiliary unlabeled videos, which are much easier to obtain, by shaping this problem as a domain adaptation (DA) problem. Although various DA techniques have been proposed in recent years, most of them have been developed only for the spatial direction. Therefore, we propose Mixed Temporal Domain Adaptation (MTDA) to jointly align frame-and video-level embedded feature spaces across domains, and further integrate with the domain attention mechanism to focus on aligning the frame-level features with higher domain discrepancy, leading to more effective domain adaptation. Finally, we evaluate our proposed methods on three challenging datasets (GTEA, 50Salads, and Breakfast), and validate that MTDA outperforms the current state-of-the-art methods on all three datasets by large margins (e.g. 6.4% gain on F1@50 and 6.8% gain on the edit score for GTEA).
A doubly salient permanent magnet motor capable of field weakening (2002)

Permanent magnet (PM) machines have been used widely for industrial applications. However, the operating speed range in most of these applications is very limited due to the difficulty of achieving field weakening in most PM structures. This difficulty is one of the major stumbling blocks to widespread application of PM machines. A new doubly salient PM motor topology is proposed in this paper which offers the possibility of low cost, high performance and robust realization of field adjustment. It is shown that by controlling the field winding, 100% field weakening and 2 p.u. torque capability can be achieved without sacrificing other performance characteristics of the machine. Hence, machines of good efficiency throughout a field weakened speed range exceeding five to one can be readily devised. This feature makes this new motor a potential candidate in future motor drive systems.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
A Structure Exploiting Branch-and-Bound Algorithm for Mixed-Integer\n Model Predictive Control (2019)

Mixed-integer model predictive control (MI-MPC) requires the solution of a\nmixed-integer quadratic program (MIQP) at each sampling instant under strict\ntiming constraints, where part of the state and control variables can only\nassume a discrete set of values. Several applications in automotive, aerospace\nand hybrid systems are practical examples of how such discrete-valued variables\narise. We utilize the sequential nature and the problem structure of MI-MPC in\norder to provide a branch-and-bound algorithm that can exploit not only the\nblock-sparse optimal control structure of the problem but that can also be warm\nstarted by propagating information from branch-and-bound trees and solution\npaths at previous time steps. We illustrate the computational performance of\nthe proposed algorithm and compare against current state-of-the-art solvers for\nmultiple MPC case studies, based on a preliminary implementation in MATLAB and\nC code.\n
Cutting Edge: The Conversion of Arginine to Citrulline Allows for a High-Affinity Peptide Interaction with the Rheumatoid Arthritis-Associated HLA-DRB1*0401 MHC Class II Molecule (2003)

Rheumatoid arthritis (RA) is genetically associated with MHC class II molecules that contain the shared epitope. These MHC molecules may participate in disease pathogenesis by selectively binding arthritogenic peptides for presentation to autoreactive CD4(+) T cells. The nature of the arthritogenic Ag is not known, but recent work has identified posttranslationally modified proteins containing citrulline (deiminated arginine) as specific targets of the IgG Ab response in RA patients. To understand how citrulline might evoke an autoimmune reaction, we have studied T cell responses to citrulline-containing peptides in HLA-DRB1*0401 transgenic (DR4-IE tg) mice. In this study, we demonstrate that the conversion of arginine to citrulline at the peptide side-chain position interacting with the shared epitope significantly increases peptide-MHC affinity and leads to the activation CD4(+) T cells in DR4-IE tg mice. These results reveal how DRB1 alleles with the shared epitope could initiate an autoimmune response to citrullinated self-Ags in RA patients.
Generation of Directly Converted Human Osteoblasts That Are Free of Exogenous Gene and Xenogenic Protein (2016)

Generation of osteoblasts from human somatic cells may be applicable in an effective transplantation therapy against bone diseases. Recently we established a procedure to directly convert human fibroblasts into osteoblasts by transducing some transcription factor genes via retroviral vectors. However, retroviral vector-mediated transduction may potentially cause tumor formation from the infected cells, thus a non-viral gene transfection method may be more preferable for preparation of osteoblasts to be used for transplantation therapy. Here, we constructed a plasmid vector encoding Oct4, Osterix, and L-Myc that were an appropriate combination of transcription factors for this purpose. Osteoblast-like phenotypes including high alkaline phosphatase (ALP) activity, bone matrix production and osteoblast-specific gene expression were induced in normal human fibroblasts that were transfected with the plasmid followed by culturing in osteogenic medium. The plasmid-driven directly converted osteoblasts (p-dOBs) were obtained even in the absence of a xenogenic protein. The plasmid vector sequence had fallen out of the p-dOBs. The cells formed deposition of calcified bodies in situ after transplantation into mice. These results strongly suggest that p-dOBs can be put into practical use for a novel cell-based therapy against bone diseases. J. Cell. Biochem. 117: 2538-2545, 2016. © 2016 Wiley Periodicals, Inc.
Interactive effects among L-carnitine, paylean (ractopamine⋅HCl), and dietary energy density on commercial finishing pig growth performance and carcass characteristics (2002)

Growth performance and carcass characteristics were evaluated on 1,104 pigs fed combinations of L-carnitine, Paylean, and added fat in a 2 Ã— 2 Ã— 2 factorial arrangement. Dietary treatments of L-carnitine (0 or 50 ppm) and fat (0 or 6%) were initiated at approximately 97 lb. Paylean (0 or 9 g/ton) was fed for the last 4 weeks prior to market. Supplementing dietary carnitine did not affect (P>0.25) growth performance of pigs between 97 to 203 lb. The addition of 6% dietary fat improved (P<0.01) ADG and F/G during this period. During the last 4 weeks of the experiment, when Paylean was fed, a carnitine Ã— Paylean interaction was observed (P<0.04) for ADG and F/G. Both carnitine and Paylean improved growth performance; however, the responses were not additive. Pigs fed added fat had improved (P<0.05) feed efficiency during the Paylean supplementation period. A carnitine Ã— Paylean interaction (P<0.03) was observed for fat thickness and percentage lean. Fat thickness decreased and lean percentage increased in pigs fed carnitine or Paylean, but the responses were not additive. Pigs fed added fat had greater (P<0.01) fat thickness and lower percentage lean than pigs not fed added fat. A carnitine Ã— Paylean Ã— fat interaction was observed (P<0.04) for longissimus muscle area. In general, adding carnitine, Paylean or fat to the diet increased longissimus muscle area; however, the responses were not fully additive. Carcass weight was greater (P<0.01) for pigs fed 6% added fat and tended (P<0.07) to be greater for pigs fed carnitine. Adding Paylean to the diet increased (P<0.04) ultimate longissimus pH and reduced drip loss as measured by the filter paper method. Similar to other experiments, adding carnitine to the diet tended to decrease drip loss (P<0.06) as measured by the suspension method. These results suggest that adding Lcarnitine and Paylean to the diet increases ADG and that L-carnitine, Paylean, and fat improve feed efficiency when fed to late finishing pigs reared in a commercial facility. These data also support our previous research that demonstrated improvements in carcass characteristics of pigs fed L-carnitine.; Swine Day, Manhattan, KS, November 14, 2002
Specific Transduction of HIV-Susceptible Cells for CCR5 Knockdown and Resistance to HIV Infection: A Novel Method for Targeted Gene Therapy and Intracellular Immunization (2009)

HIV-1 gene therapy offers a promising alternative to small molecule antiretroviral treatments and current vaccination strategies by transferring, into HIV-1-susceptible cells, the genetic ability to resist infection. The need for novel and innovative strategies to prevent and treat HIV-1 infection is critical due to devastating effects of the virus in developing countries, high cost, toxicity, generation of escape mutants from antiretroviral therapies, and the failure of past and current vaccination efforts. As a first step toward achieving this goal, an HIV-1-susceptible cell-specific targeting vector was evaluated to selectively transfer, into CCR5-positive target cells, an anti-HIV CCR5 shRNA gene for subsequent knockdown of CCR5 expression and protection from HIV-1 infection. Using a ZZ domain/monoclonal antibody-conjugated Sindbis virus glycoprotein pseudotyped lentiviral vector, here we demonstrate the utility of this strategy for HIV-1 gene therapy by specifically targeting HIV-1-susceptible cells and engineering these cells to resist HIV-1 infection. CCR5-positive human cells were successfully and specifically targeted in vitro and in vivo for transduction by a lentiviral vector expressing a highly potent CCR5 shRNA which conferred resistance to HIV-1 infection. Here we report the initial evaluation of this targeting vector for HIV-1 gene therapy in a preexposure prophylactic setting.
Securing Neighbor Discovery Proxy: Problem Statement (2010)

Neighbor Discovery Proxy is used to provide an address presence on a
link from nodes which are no themselves present. It allows a node to
receive packets directed at its address by allowing another device to
Neighbor advertise on its behalf. Neighbor Discovery Proxy is used in
Mobile IPv6 and related protocols to provide reachability from nodes
on the home network when a Mobile Node is not at home, by allowing the
Home Agent to act as proxy. It is also used as a mechanism to allow a
global prefix to span multiple links, where proxies act as relays for
Neighbor discovery messages. Neighbor Discovery Proxy currently
cannot be secured using SEND. Today, SEND assumes that a node
advertising an address is the address owner and in possession of
appropriate public and private keys for that node. This document
describes how existing practice for proxy Neighbor Discovery relates
to Secured Neighbor Discovery.
Developmental Progress of the Nanoparticle Field Extraction Thruster (2008)

The Nanoparticle Field Extraction Thruster (NanoFET) is an electrostatic accelerator technology currently under development at the University of Michigan to accelerate micro-/ nano-particles. The concept exists in two configurations: the liquid configuration stores and transports particulate propellant using microfluidics while the dry configuration eliminates the liquid feed system in favor of particle transport via piezoelectric actuators. Microgravity flight tests of the liquid-NanoFET concept indicate good agreement with theory regarding the threshold electric field for liquid surface instabilities. This threshold electric field was observed to increase in low Bond number systems as the channel diameter decreased and appeared to be governed by the largest characteristic channel orifice dimension. Particle liftoff and extraction from both liquidand air-filled reservoirs were also demonstrated in the microgravity environment. On the ground, preliminary experiments showed that particle liftoff electric fields could be reduced with the application of inertial accelerations from piezoelectrics to the charging electrode. Both the recent microgravity and ground test results for the NanoFET concept are presented.
Characterization of endogenously circulating IGFBP-4 fragments—Novel biomarkers for cardiac risk assessment (2015)

Recent findings show that circulating N- and C-terminal fragments of IGF-binding protein-4 (NT-IGFBP-4 and CT-IGFBP-4) can be utilized as biomarkers for cardiac risk assessment in acute coronary syndrome (ACS) patients. The fragments are thought to be the products of pregnancy-associated plasma protein A (PAPP-A)-dependent proteolysis. Two immunoassays for the measurement of IGFBP-4 fragments have been proposed. However, properties of the endogenous IGFBP-4 fragments that could influence the performance of the immunoassays were still not investigated. NT- and CT-IGFBP-4 were extracted from pooled ACS plasma using affinity purification, and their concentrations were measured using sandwich immunoassays utilizing antibodies specific to their proteolytic neo-epitopes or internal epitopes. The extracted fragments were characterized by Western blots (WB) and mass-spectrometry. ACS plasma samples were analyzed by size exclusion chromatography (SEC). Immunoassays utilizing the neo-epitope-specific and the internal epitope-specific antibodies measured equal concentrations of the analyte in the endogenous IGFBP-4 fragments preparations. Only the 18 kDa NT-IGFBP-4 and 14 kDa CT-IGFBP-4 were detected in the WB analysis. Using mass-spectrometry, peaks corresponding to intact non-truncated and non-modified NT-IGFBP-4 (14626 Da) and CT-IGFBP-4 (11346 Da) were observed. The absence of complexed forms of IGFBP-4 in patients' plasma was demonstrated using SEC. Endogenous NT- and CT-IGFBP-4 from ACS patients' plasma correspond to the PAPP-A-derived IGFBP-4 fragments and do not undergo any truncation, modification, or complex formation in the patients' blood. Because of the demonstrated intact state of the circulating IGFBP-4 fragments, the neo-epitope-specific immunoassays perform reliably, allowing further clinical validation of these novel biomarkers.
Interactions between Papillomavirus L1 and L2 Capsid Proteins (2003)

The human papillomavirus (HPV) capsid consists of 360 copies of the major capsid protein, L1, arranged as 72 pentamers on a T=7 icosahedral lattice, with substoichiometric amounts of the minor capsid protein, L2. In order to understand the arrangement of L2 within the HPV virion, we have defined and biochemically characterized a domain of L2 that interacts with L1 pentamers. We utilized an in vivo binding assay involving the coexpression of recombinant HPV type 11 (HPV11) L1 and HPV11 glutathione S-transferase (GST) L2 fusion proteins in Escherichia coli. In this system, L1 forms pentamers, GST=L2 associates with these pentamers, and L1+L2 complexes are subsequently isolated by using the GST tag on L2. The stoichiometry of L1:L2 in purified L1+L2 complexes was 5:1, indicating that a single molecule of L2 interacts with an L1 pentamer. Coexpression of HPV11 L1 with deletion mutants of HPV11 L2 defined an L1-binding domain contained within amino acids 396 to 439 near the carboxy terminus of L2. L2 proteins from eight different human and animal papillomavirus serotypes were tested for their ability to interact with HPV11 L1. This analysis targeted a hydrophobic region within the L1-binding domain of L2 as critical for L1 binding. Introduction of negative charges into this hydrophobic region by site-directed mutagenesis disrupted L1 binding. L1-L2 interactions were not significantly disrupted by treatment with high salt concentrations (2 M NaCl), weak detergents, and urea concentrations of up to 2 M, further indicating that L1 binding by this domain is mediated by strong hydrophobic interactions. L1+L2 protein complexes were able to form virus-like particles in vitro at pH 5.2 and also at pH 6.8, a pH that is nonpermissive for assembly of L1 protein alone. Thus, L1/L2 interactions are primarily hydrophobic, encompass a relatively short stretch of amino acids, and have significant effects upon in vitro assembly.