gebhart/patrepeval huggingface dataset:
Highly Sensitive Biomolecular Fluorescence Detection Using Nanoscale ZnO Platforms (2006)

Fluorescence detection is currently one of the most widely used methods in the areas of basic biological research, biotechnology, cellular imaging, medical testing, and drug discovery. Using model protein and nucleic acid systems, we demonstrate that engineered nanoscale zinc oxide structures can significantly enhance the detection capability of biomolecular fluorescence. Without any chemical or biological amplification processes, nanoscale zinc oxide platforms enabled increased fluorescence detection of these biomolecules when compared to other commonly used substrates such as glass, quartz, polymer, and silicon. The use of zinc oxide nanorods as fluorescence enhancing substrates in our biomolecular detection permitted sub-picomolar and attomolar detection sensitivity of proteins and DNA, respectively, when using a conventional fluorescence microscope. This ultrasensitive detection was due to the presence of ZnO nanomaterials which contributed greatly to the increased signal-to-noise ratio of biomolecular fluorescence. We also demonstrate the easy integration potential of zinc oxide nanorods into periodically patterned nanoplatforms which, in turn, will promote the assembly and fabrication of these materials into multiplexed, high-throughput, optical sensor arrays. These zinc oxide nanoplatforms will be extremely beneficial in accomplishing highly sensitive and specific detection of biological samples involving nucleic acids, proteins and cells, particularly under detection environments involving extremely small sample volumes of ultratrace-level concentrations.
SEPARATION TECHIQUES IN SATURATION ANALYSIS (1974)

Journal Article SEPARATION TECHIQUES IN SATURATION ANALYSIS Get access J. G. RATCLIFFE, M.A. M.Sc. D.M. J. G. RATCLIFFE, M.A. M.Sc. D.M. Radioimmunoassay Unit, Department of Pathological Biochemistry Royal InfirmaryGlasgow Search for other works by this author on: Oxford Academic PubMed Google Scholar British Medical Bulletin, Volume 30, Issue 1, January 1974, Pages 32–37, https://doi.org/10.1093/oxfordjournals.bmb.a071164 Published: 01 January 1974
Catalytic Dehydrogenative Coupling of Secondary Silanes Using Wilkinson's Catalyst (2001)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTCatalytic Dehydrogenative Coupling of Secondary Silanes Using Wilkinson's CatalystLisa Rosenberg, Colin W. Davis, and Junzhi YaoView Author Information Department of Chemistry, University of Manitoba Winnipeg, Manitoba, Canada R3T 2N2 Cite this: J. Am. Chem. Soc. 2001, 123, 21, 5120–5121Publication Date (Web):May 5, 2001Publication History Received20 February 2001Revised12 April 2001Published online5 May 2001Published inissue 1 May 2001https://pubs.acs.org/doi/10.1021/ja015697ihttps://doi.org/10.1021/ja015697irapid-communicationACS PublicationsCopyright © 2001 American Chemical SocietyRequest reuse permissionsArticle Views2922Altmetric-Citations84LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Catalysts,Chemoselectivity,Group 14 compounds,Hydrogen,Inorganic compounds Get e-Alerts
The impact of the ATM concept on video coding (1988)

The induction of the asynchronous transfer mode (ATM) concept may significantly influence the coding of video services for broadband networks. The authors show how the absence of a physical channel structure and the ability to switch bursty traffic can be used to enhance video coding. Packetization defects and their impact on picture quality, coding algorithms, and synchronization schemes are studied. The authors describe variable-bit-rate coding and report on the results obtained with a hardware implementation of a variable-bit-rate video codec. Statistical multiplexing gain figures are given. The influence of cell loss on image quality is discussed and simulation results are given. A layered coding model offering good cell loss concealment properties and high flexibility is described.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Inhibition of Ileal Na<sup>+</sup>/Bile Acid Cotransporter by S-8921 Reduces Serum Cholesterol and Prevents Atherosclerosis in Rabbits (1998)

The ileal Na+/bile acid cotransporter (IBAT) plays an important role in the enterohepatic circulation of bile acids. We investigated the effects of IBAT inhibition on the maintenance of serum cholesterol level by using a novel IBAT inhibitor, S-8921, in rabbits. Administration of S-8921 by its incorporation into the diet (0.01% to 0.1%) for 1 to 2 weeks in heterozygous Watanabe heritable hyperlipidemic rabbits decreased serum cholesterol by 29% to 37% and increased fecal excretion of measured bile acids by 60% to 180% compared with control rabbits. Liver microsomal cholesterol 7alpha-hydroxylase and 3-hydroxy-3-methylglutaryl coenzyme A reductase activities were increased by 75% to 84% and 84% to 89%, respectively, with S-8921 treatment. S-8921 administration (0.1% in the diet) to normal New Zealand White rabbits for 2 weeks resulted in increased hepatic low density lipoprotein receptor expression, which was assessed by Northern blot analysis. In cholesterol-fed New Zealand White rabbits, S-8921 treatment (0.003% to 0.1% in the diet) for 10 weeks dose-dependently inhibited the development of hypercholesterolemia. It also inhibited the accumulation of cholesterol in the aortic arch and reduced the severity of coronary atherosclerosis. These results indicate that IBAT inhibition by S-8921 affects serum cholesterol, liver enzymes, low density lipoprotein receptor activity, and atherosclerosis in the same manner as bile acid sequestrants. We suggest that an IBAT inhibitor such as S-8921 could be useful in the treatment of hypercholesterolemia.
Constructive Interference Optimization for Hybrid Beamforming with Data-aided Symbol-level Precoding in Multi-user MISO System (2019)

The data-aided symbol-level precoding scheme has been known as a useful means of improving the system efficiency in multi-user multi-input single-output (MISO) system, especially with a full digital beamforming. As the number of antenna elements increases, it must be extended to hybrid beamforming with a limited number of radio frequency (RF) chains, while degrading its performance. In this paper, we provide a solution approach to the minimum mean-square estimation (MMSE)-based constructive interference optimization (CIO) problem for hybrid beamforming. As it has to resort to the local optimal solution with hybrid beamforming with a limited number of RF chains, its performance turns out to be much worse than that of full digital beamforming. However, its capacity has been shown to exceed that of the existing scheme, indicating that the data-aided symbol-level precoding would be still useful for a massive number of antenna elements in practice.
Wide two-dimensional field laser ray-tracing aberrometer (2012)

There is an increasing interest in measuring the peripheral optical quality of the eye. Optical aberrations have been studied extensively in the center of the visual field due to the development of Hartmann-Shack wavefront sensor. However, experimental data of the peripheral field of view are still scarce, partly due to the fact that this evaluation presents various challenges. Here, we propose a novel device based on the laser ray-tracing (LRT) aberrometer, which is well suited for measuring the off-axis aberrations. The proposed instrument is able to measure a wide (±40°) 2D visual field and is based on three main design principles: spiral-shaped sampling of the visual field, real-time detection of the eye's entrance pupil, and automatic shaping and delivering of the ray bundle that optimally samples the eye pupil. We present experimental data obtained on 11 healthy subjects and a novel analysis based on a 2D quadratic model of the aberrations as a function of visual field and azimuth. The obtained results are consistent with previous findings.
Acid‐Catalyzed Dehydration of Fructose into 5‐Hydroxymethylfurfural by Cellulose‐Derived Amorphous Carbon (2012)

Carbonaceous solid (CS) catalysts with --SO₃H, --COOH, and phenolic --OH groups were prepared by incomplete hydrothermal carbonization of cellulose followed by either sulfonation with H₂SO₄ to give carbonaceous sulfonated solid (CSS) material or by both chemical activation with KOH and sulfonation to give activated carbonaceous sulfonated solid (a-CSS) material. The obtained carbon products (CS, CSS, and a-CSS) were amorphous; the CSS material had a small surface area (<0.5 m² g⁻¹) and a high --SO₃H group concentration (0.953 mmol g⁻¹), whereas the a-CSS material had a large surface area (514 m² g ⁻¹) and a low --SO₃H group concentration (0.172 mmol g⁻¹). The prepared materials were evaluated as catalysts for the dehydration of fructose to 5-hydroxymethylfurfural (5-HMF) in the ionic liquid 1-butyl-3-methylimidazolium chloride ([BMIM][Cl]). Remarkably high 5-HMF yields (83 %) could be obtained efficiently (80 °C and 10 min reaction time). CSS and a-CSS catalysts had similar catalytic activities and efficiencies for the conversion of fructose to 5-HMF in [BMIM][Cl]; this could be explained by the trade-off between --SO₃H group concentration (high for CSS) and surface area (high for a-CSS). The cellulose-derived catalysts and ionic liquid exhibited constant activity for five successive recycles, and thus, the methods developed provide a renewable strategy for biomass conversion.
Structure of a G-protein-coupling Domain of a Muscarinic Receptor Predicted by Random Saturation Mutagenesis (1996)

The third intracellular loop (i3) plays a critical role in the coupling of many receptors to G-proteins. In muscarinic receptor subtypes, the N- and C-terminal regions (Ni3 and Ci3) of this loop are sufficient to direct appropriate G-protein coupling. The relative functional contributions of all amino acids within Ni3 was evaluated by constructing libraries of m5 muscarinic receptors containing random mutations in Ni3 and screening them using high throughput assays based on ligand-dependent transformation of NIH 3T3 cells. In receptors that retained a wild type phenotype, the pattern of functionally tolerated substitutions is consistent with the presence of three turns of an alpha helix extending from the transmembrane domain. All of the amino acid positions that tolerate radical substitutions face away from a conserved hydrophobic face that ends with an arginine, and helix-disrupting proline substitutions were not observed. All of the mutant receptors with significantly compromised phenotypes had amino acid substitutions in residues predicted to form the hydrophobic face. Similar data from the Ci3 region (Burstein, E. S., Spalding, T. A., Hill-Eubanks, D., and Brann, M. R. (1995) J. Biol. Chem. 270, 3141-3146) are consistent with the presence of a single helical turn extending from the transmembrane domain, with an alanine that defines G-protein affinity. Functionally critical residues of Ni3 and Ci3 are predicted to be in close proximity where they form the G-protein-coupling domain.
Tubulogenesis by microvascular endothelial cells is mediated by vascular endothelial growth factor (VEGF) in renal cell carcinoma (1997)

VEGF, which is produced and released from RCC cells, may elicit tumour angiogenesis by inducing microvessel tubulogenesis in patients with RCC. The co-culture system may be useful for screening inhibitors of tumour angiogenesis in RCC.
Channel aware scheduling for multiple antenna multiple access channels (2004)

In this paper, we study the optimum transmission strategy of the multiple access channel in a cellular system in which the base station has multiple antennas. Recently, scheduling algorithms incorporating both the physical and data link layer were proposed e.g. in H. Boche and M. Wiczanowski, (2003), M. J. Neely, et al., (Feb. 2003), E. M. Yeh, (2002), for the multiple antenna case. In this work, we study the scheduling in a SIMO MAC under arbitrary ergodic fading. We assume that the instantaneous channel vector realizations as well as the buffer sizes of all mobiles are known at the base station. The base station performs successive interference cancellation. We propose the optimal scheduling policy. In order to get rid of the time-sharing argument, we define a spatial capacity region in which all rate tupels are achieved by spatial multiplexing only without time-sharing. Finally, we connect the capacity region of the SIMO MAC on the physical layer with the stability region of the corresponding queueing system on the data link layer. It is shown that all bit arrival rate vector lying in the capacity region of the SIMO MAC are achievable, too. All theoretical results are illustrated.
20-Gb/s Adaptively Modulated Optical OFDM Transmission over 20-km Single Fiber Loopback WDM PON based on Separated I/Q Baseband Delivery using 1 GHz RSOAs (2012)

20-Gb/s optical OFDM transmission with separated I/Q baseband delivery over 20-km single fiber loopback link was demonstrated using remotely-fed 1-GHz RSOAs in WDM-PON. Adaptive loading algorithm without Hermitian Symmetry was applied to optimize the performance.
900V SiC Based, Hybrid, Multilevel DC/DC Topology for 1500VDC PV Application (2019)

Very compact, highly efficient, hybrid DC/DC topology, consisted of a standard interleaved boost converter followed by a multilevel Resonant Switched Capacitor Converter (RSCC), that employs 900V Silicon-Carbide (SiC) devices, for use in emerging, two-stage, grid connected 1500V Photo-Voltaic (PV) systems is presented in this paper. Operation principle of this topology is described: controllability of its output voltage and power processing distribution among its stages in function of input voltage. Depending on operating region, certain amount of input power is not processed, but it is transferred directly to the output. This behaviour increases significantly efficiency of the analyzed topology, compared to the solutions presented in literature. The presented topology obtains full ZVS transitions in all operating conditions, even without load. ZVS transitions and employed SiC MOSFETs are the key elements for the high converter efficiency. Triangular Conduction Mode (TCM) in boost stage and resonant switching technique in RSCC part are used to obtain ZVS transitions in all the switches under wide range of loads and PV voltages. Results of multivariable optimization (pareto front) for the presented topology are provided. Previously described theoretical analysis is fully experimentally confirmed by building and measuring a 10kW prototype of 8.26kW/kg of specific power and 404.6cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sup> of volume occupied only by power stage components. Euro efficiency of 99.48% in nominal input/output voltage conditions is achieved.
Binary linear-time erasure decoding for non-binary LDPC codes (2009)

In this paper, we first introduce the extended binary representation of non-binary codes, which corresponds to a covering graph of the bipartite graph associated with the non-binary code. Then we show that non-binary codewords correspond to binary codewords of the extended representation that further satisfy some simplex-constraint: that is, bits lying over the same symbol-node of the non-binary graph must form a codeword of a simplex code. Applied to the binary erasure channel (BEC), this description leads to a binary erasure decoding algorithm of non-binary LDPC codes, whose complexity depends linearly on the cardinality of the alphabet. We also give insights into the structure of stopping sets for non-binary LDPC codes, and discuss several aspects related to upper-layer FEC applications.
ON ORDER EQUIVALENCES BETWEEN DISTANCE AND SIMILARITY MEASURES ON SEQUENCES AND TREES (2012)

Both ?distance? and ?similarity? measures have been proposed for the comparison of sequences and for the comparison of trees, based on scoring mappings, and the paper concerns the equivalence or otherwise of these. These measures are usually parameterised by an atomic ?cost? table, defining label-dependent values for swaps, deletions and insertions. We look at the question of whether orderings induced by a ?distance? measure, with some cost-table, can be dualized by a ?similarity? measure, with some other cost-table, and vice-versa. Three kinds of orderings are considered: alignment-orderings, for fixed source S and target T , neighbour-orderings, where for a fixed S, varying candidate neighbours Ti are ranked, and pair-orderings, where for varying Si ,and varying Tj , the pairings Si , Tj are ranked. We show that (1) alignment-orderings by distance can be dualized by similarity, and vice-versa; (2) neigbour-ordering and pair-ordering by distance can be dualized by similarity; (3) neighbour-ordering and pair-ordering by similarity can sometimes not be dualized by distance. A consequence of this is that there are categorisation and hierarchical clustering outcomes which can be achieved via similarity but not via distance
Detection of social signals for recognizing engagement in human-robot interaction (2017)

Detection of engagement during a conversation is an important function of human-robot interaction. The level of user engagement can influence the dialogue strategy of the robot. Our motivation in this work is to detect several behaviors which will be used as social signal inputs for a real-time engagement recognition model. These behaviors are nodding, laughter, verbal backchannels and eye gaze. We describe models of these behaviors which have been learned from a large corpus of human-robot interactions with the android robot ERICA. Input data to the models comes from a Kinect sensor and a microphone array. Using our engagement recognition model, we can achieve reasonable performance using the inputs from automatic social signal detection, compared to using manual annotation as input.
Chemoenzymatic Strategy for the Synthesis of Site-Specifically Labeled Immunoconjugates for Multimodal PET and Optical Imaging (2014)

The complementary nature of positron emission tomography (PET) and optical imaging (OI) has fueled increasing interest in the development of multimodal PET/OI probes that can be employed during the diagnosis, staging, and surgical treatment of cancer. Due to their high selectivity and affinity, antibodies have emerged as promising platforms for the development of hybrid PET/OI agents. However, the lack of specificity of many bioconjugation reactions can threaten immunoreactivity and lead to poorly defined constructs. To circumvent this issue, we have developed a chemoenzymatic strategy for the construction of multimodal PET/OI immunoconjugates that have been site-specifically labeled on the heavy chain glycans. The methodology consists of four steps: (1) the enzymatic removal of the terminal galactose residues on the heavy chain glycans; (2) the enzymatic incorporation of azide-bearing galactose (GalNAz) residues into the heavy chain glycans; (3) the strain-promoted click conjugation of chelator- and fluorophore-modified dibenzocyclooctynes to the azide-modified sugars; and (4) the radiolabeling of the immunoconjugate. For proof-of-concept, a model system was created using the colorectal cancer-targeting antibody huA33, the chelator desferrioxamine (DFO), the positron-emitting radiometal (89)Zr, and the near-infrared fluorescent dye Alexa Fluor 680. The bioconjugation strategy is robust and reproducible, reliably producing well-defined and immunoreactive conjugates labeled with (89)Zr, Alexa Fluor 680, or an easily and precisely tuned mixture of the two reporters. In in vivo PET and fluorescence imaging experiments, a hybrid (89)Zr- and Alexa Fluor 680-labeled huA33 conjugate displayed high levels of specific uptake (>45% ID/g) in athymic nude mice bearing A33 antigen-expressing SW1222 colorectal cancer xenografts.
Simultaneous staining of exponentially growing versus plateau phase cells with the proliferation‐associated antibody Ki‐67 and propidium iodide: analysis by flow cytometry (1987)

The antibody Ki-67, which detects proliferating cells, was used in combination with propidium iodide, a DNA-specific dye. The double-staining method allowed discrimination of cells in the phases of the cell cycle as well as the recognition of Ki-67 staining characteristics. Suspension cultures of U937 cells were measured in exponential growth and plateau phase in nutritional deprivation. The fraction of Ki-67 positive cells was nearly 100% 2 days after dilution and 46% 7 days after dilution of the cultures. Stathmokinetic measurements with colchicine and flow cytometry measurements with the BrdU-Hoechst technique yielded close to 100% proliferation at day 2 but only 18% and 6%, respectively, at day 7. The discrepancy between Ki-67 results and the results of the two other methods is considered to be a characteristic of nutritionally deprived cells.
Ultrafast wavelength and polarization insensitive all-optical clock recovery (2002)

Wavelength and polarization insensitive all-optical clock recovery based on a two-section gain-coupled DFB laser and cross gain conversion has been demonstrated. The lockup time of clock recovery is less than 1 ns.
Three dimensional IC for high performance image signal processor (1987)

The three-dimensional (3-D) image processing test IC designed with parallel processing architecture is fabricated. The device consists of 5-by-5 array of photosensors, 2-bit CMOS A-to-D converters, 40 arithmetic logic units (ALU) and shiftregisters arranged in a 3-layer structure. The total operation from photosensor on top layer to ALU on bottom layer is confirmed, and it is also demonstrated the feasibility of very high speed system operation with the implement of parallel processing. This device gives a clear image of the intelligent image processor on one chip as a future application of 3-D ICs.
Global-Scale Secure Multiparty Computation (2017)

We propose a new, constant-round protocol for multi-party computation of boolean circuits that is secure against an arbitrary number of malicious corruptions. At a high level, we extend and generalize recent work of Wang et al. in the two-party setting. Namely, we design an efficient preprocessing phase that allows the parties to generate authenticated information; we then show how to use this information to distributively construct a single "authenticated" garbled circuit that is evaluated by one party.