gebhart/patrepeval huggingface dataset:
58 HANDMADE CLONING IN TRANS-SPECIES NT: CULTURE MEDIUM HAS AN EFFECT ON THE ABILITY OF RECONSTRUCTED BOVINE-MURINE EMBRYOS TO DEVELOP BEYOND THE 8-CELL STAGE (2004)

The objectives of studies of trans-species nuclear transfer (NT) include epigenetic reprogramming and stem cell technology. The present study evaluated the effect of culture media on the development of reconstructed bovine-murine embryos. Bovine NT served as a technical control. The NT embryos were produced with the hand made cloning (HMC) technique (Vajta G et al. 2003 Biol. Reprod. 68, 571–578), and to our knowledge, this is the first report on the application of HMC in trans-species NT. Abattoir-derived bovine oocytes were matured for 21 h and enucleated by hand as described (Vajta G et al. 2003). Bovine cytoplasts were fused with either bovine granulosa cells or murine fetal fibroblasts. The bovine NT embryos were cultured for 7 days in modified SOFaaci (Holm P et al. 1999 Theriogenology 52, 683–700) containing either 5% FBS (8 trials) or 4 mg mL-1 fatty acid free albumin (FAFBSA, 6 trials). Bovine-murine NT embryos were cultured for 4.5 days in SOFaaci + FAFBSA (5 trials), or for the first 12 h in SOFaaci + FAFBSA and until 4.5 days in KSOMaa (Biggers JD 1991 J. Reprod. Fertil. 91, 543) containing 1 mg mL-1 embryo-tested BSA (4 trials). The results are shown in Table 1. In bovine NT embryos, both cleavage (day 2) and day 7 blastocyst rates were significantly improved was SOFaaci + FAFBSA was used as culture medium. Culture medium did not affect the cleavage rate of bovine-murine NT embryos at 12–16 h after start of culture. The development of reconstructed bovine-murine embryos beyond the 8-cell stage was significantly improved when SOFaaci + FAFBSA was replaced with KSOMaa + BSA after 12 h culture. Fourteen of a total of 464 (3.0%) Day 4.5 bovine-murine reconstructed embryos reached early morula stage with signs of compaction. The study showed that the development of the reconstructed NT embryos was significantly affected by the culture medium. Contrary to earlier findings (Park SH et al. 2004 Mol. Reprod. Dev. 68, 25–34), the bovine-murine reconstructed embryos developed beyond 8-cell stage, even until early compaction. The gene expression of species-specific and development-related genes of the reconstructed embryos is under characterization. Table 1. Effect of culture medium on cleavage and development of reconstructed NTt embryos Professor Gabor Vajta is greatly acknowledged for his contribution in establishing the HMC technique in our laboratory.
Restriction Fragment Length Polymorphism Assays of Clinical Isolated Human Cytomegalovirus Strain Genomic DNA (1996)

Ten clinical isolated strains of Human cytomegalovirus(HCMV) were obtained from 73 urine specimens of different people.Isolations from urine were carried out in human embryolung fibroblasts. Viral isolates were passaged four times.HCMV DNAs of laboratory strain AD 169 and 10 clinical isolated strains were extracted with Hirt method,digested with each of the restriction enzymes EcoRI,HindⅢ.Comparison of restriction fragment length polymorphism(RFLP) of AD 169 and isolated strains were made by hybridizing digested DNA with 32 P labeled with HCMV HindⅢ cloned subgenomic fragments as the probe (pCM1035,pCM1015). pCM1035 is located in the joining region between the long(L) and short (S) unique sequences of the virus (L S junction );pCM1015 is located in the terminal sequences of the virus.The results showed the genomic high degree of homology existed among all strains and the variable restriction site was in the L S junction and terminal portion.The RFLP patterns of the clinical isolates which did not have relation in epidemiology were different,but the patterns of clinical isolates related in epidemiology were quite similar.Polymorphism frequently occurred in this case of EcoRI digested fragment hybridized with the probe of pCM1035.Southern hybridization of HCMV isolations is useful to resarches into the molecular epidemiology and pathogenesis of HCMV infection.
Construction of a Keggin heteropolyacid/Ni-MOF catalyst for esterification of fatty acids (2021)

This work reports the one-pot solvothermal synthesis of a Keggin heteropolyacid (phosphomolybdic acid, tungstophosphoric acid, or silicotungstic acid) immobilized on Ni-MOF composite catalysts for esterification of fatty acids, and the composites were further analyzed by XRD, FTIR, NH<sub>3</sub>-TPD, SEM, TEM, N<sub>2</sub> adsorption/desorption, and XPS. Among the contrastive syntheses (<i>i.e.</i>, HPW/Ni-MOF, HSiW/Ni-MOF, and HPMo/Ni-MOF), HPMo/Ni-MOF exhibits the most active catalyst toward fatty acids esterification, and the characterization results also revealed that HPMo/Ni-MOF has a strong acidity, large specific surface area, and appropriate average pore size. More significantly, this catalyst exhibits a good catalytic performance (86.1% conversion) during esterification under the optimized reaction conditions, and the HPMo/Ni-MOF catalyst can remain stable after the tenth cycle with a conversion of 73.5%. Intriguingly, the esterification reaction kinetics was studied, and the activation energy was found to be 64.6 kJ mol<sup>-1</sup>. The results indicated that the esterification of fatty acids using the HPMo/Ni-MOF catalyst is a chemically controlled reaction.
Design of a Muscle-Powered Extra-Aortic Counterpulsation Device for Long-Term Circulatory Support (2017)

While great strides continue to be made in the treatment of congestive heart failure using mechanical ventricular assist devices (VADs), several longstanding difficulties associated with pumping blood continue to limit their long-term use. Among the most troublesome has been the persistent risk of clot formation at the blood-device interface, which generally requires VAD recipients to undergo costly — and potentially dangerous — anticoagulation therapy for the duration of the implant. Another serious and persistent problem with long-term use of these pumps is the increased risk of infection associated with the use of percutaneous drivelines. To address these issues we are currently exploring a new approach to blood pump design that aims to solve both these problems by avoiding them altogether. Toward that end, we propose to harness the body’s own endogenous energy stores in order to eliminate the need to transmit energy across the skin. Further, we intend to transfer the energy from this internal power source to the circulation without contacting the blood to obviate the thrombogenic risks imposed by devices placed directly into the bloodstream. To power the implant we will employ a device developed previously by our group called a muscle energy converter (MEC), shown in Figure 1. The MEC is, in essence, an implantable hydraulic actuator powered by the latissimus dorsi (LD) muscle with the capacity to transmit up to 1.37 joules of contractile work per stroke [1]. By training the muscle to express fatigue-resistant oxidative fibers and stimulating the LD to contract in coordination with the cardiac cycle, the MEC captures and transmits this contractile energy as a high-pressure low-volume (5 cc) hydraulic pulse that can be used, in principle, to actuate an implanted pulsatile blood pump. The goal of this research is to use the low-volume output of the MEC to drive a polymer-based aortic compression device for long-term circulatory support. In this context it is important to note that the idea of applying a counterpulsation device around the ascending aorta is not new. Indeed, this approach has been validated by clinical trials recently completed by Sunshine Heart Inc. showing that displacing 20 cc of blood at the aortic root has significant therapeutic benefits [2]. Unfortunately, while the pneumatic ‘C-Pulse’ device solves the blood-contacting problem, it suffers from the same limitations as traditional VADs — i.e., driveline infections. The device described here achieves the same volumetric displacement as the SSH device via geometric amplification of MEC outputs. Thus, through this mechanism we believe the low-volume power output of the MEC can be used to support heart failure patients while addressing the major limitations associated with long-term VAD use.
Les vaincues de l'an 40 (2001)

La faim, la misere, les privations et les humiliations : tel est le lot quotidien de la majorite des sous l'Occupation, souvent propulsees chefs de famille depuis la debâcle de juin 1940. Pour elles, la guerre est un combat ordinaire. Pour d'autres femmes, juives, c'est surtout une lutte pour la survie : echapper aux rafles des nazis, sortir un jour des camps de la mort. Alors que Vichy prone l'ideal de la mere au foyer, certaines femmes, refusant la soumission et la collaboration, s'engagent dans la Resistance ou rejoignent les forces armees. Agents de liaison, cantinieres, infirmieres ou combattantes, ces heroines, anonymes pour la plupart, accederont tardivement a la reconnaissance que le pays leur doit. Emaille de portraits de grandes femmes et riche de plus de 200 photographies et illustrations rares et originales, ce livre aborde des themes meconnus comme la prostitution organisee ou les violences sexuelles. Parce que l'histoire de la Seconde Guerre mondiale est trop souvent conjuguee au masculin, il propose une relecture du conflit a travers le prisme du deuxieme sexe, sur le long chemin de l'emancipation.
Design and Characterisation of High-Q Solidly-Mounted Bulk Acoustic Wave Filters (2007)

In this paper Bulk-Acoustic-Wave (BAW) resonators with high Q-factor 𝚀 <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">a</sub> at anti-resonance are demonstrated based on a redesigned reflector stack and a lateral design with a guard ring at the border of the resonator. Experimentally a clear maximum of 𝚀 <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">a</sub> together with optimal suppression of spurious modes in the inductive region is observed for a guard ring width of about 7 <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">μ</i> m. It is demonstrated that by measuring resonators of different size and aspect ratio, bulk and edge loss mechanisms can be differentiated. Furthermore, the most relevant part of the dispersion curve for the main acoustic mode can be extracted. The observed trends are confirmed by a recently developed finite element model.
Intelligent bridges and routers-a proposal for the LAN/MAN/B-ISDN environment (2003)

An interworking concept called intelligent interworking units (I/sup 2/Us) is proposed and applied to the interconnection of IEEE 802.X LANs, IEEE 802.6 distributed-queue dual bus (DQDB) metropolitan area networks (MANs), and asynchronous-transfer mode (ATM)-based broadband integrated services digital network (B-ISDN). Interworking units (IWUs) are necessary to provide flexible communication capabilities among all the above mentioned network types. Currently IWUs use a fixed layering scheme and are thus classified as bridges, routers, or gateways. The I/sup 2/U concept enhances conventional IWUs to allow for more sophisticated operation in a high-speed environment. I/sup 2/Us dynamically select the layers for interworking according to the received type of data. This avoids encapsulation at the source IWU and makes use of Layer 3 network functions only when needed. An overview of the provision of connectionless services in the three network types is given. The I/sup 2/U concept for the interconnection of LANs via MANs and of MANs via the B-ISDN is described.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Motion‐robust diffusion compartment imaging using simultaneous multi‐slice acquisition (2018)

The proposed approach has the potential to extend routine use of SMS DCI in very challenging populations, such as young children, newborns, and non-cooperative patients.
Two-dof non-metal manipulator with pneumatic stepping actuators for needle puncturing inside open-type MRI (2010)

The magnetic resonance imaging (MRI) system is useful in intraoperative surgical treatment. Many manipulators for the clinical application on an MRI bed are reported. In many cases, piezoceramic motors have been used for these manipulators. However, a piezoceramic motor produces an electromagnetic wave that influences an MRI image. In this paper, we develop an MRI-safe pneumatic stepping actuator and an MRI-safe needle guiding manipulator. This actuator and the manipulator are made of only resin. In addition, this pneumatic stepping actuator can be operated at a distance of 8 [m] from the compressor. By using these devices, we construct an MRI-safe system in which there are no metallic or electronic parts inside the MRI room. From the result of the accuracy evaluation experiment, it is evident that this manipulator inserts the needle at the high accuracy. Further, we confirm that this system has a high magnetic resonance (MR) compatibility.
Double Hidden Layer Output Feedback Neural Adaptive Global Sliding Mode Control of Active Power Filter (2019)

In this paper, a self-regulated double hidden layer output feedback neural network (DHLFNN) is presented to control an active power filter (APF) system as a current controller, which is conducive to the improvement of the response characteristic and power quality. First, a global sliding mode controller is introduced because it is effective in achieving overall robustness during the system response. A new output feedback neural structure that has two hidden layers is proposed to make the parameters adaptively adjust themselves and stabilize to their best values. A higher accuracy and stronger generalization ability can be also obtained by reducing the number of network weights and accelerating the network training speed owing to the strong fitting and presentation ability of two-layer activation functions. Furthermore, the designed feedback loops of the neural network play a significant role in possessing associative memory and rapid system convergence. This proposed double hidden layer output feedback neural based global sliding mode controller is simulated on the model of APF and the results show the excellent static and dynamic properties. Experimental results under three cases and comparisons are provided using a fully digital control system to validate the superior performance of the proposed DHLFNN controller.
Damage Classification Structural Health Monitoring in Bolted Structures Using Time-frequency Techniques (2009)

The analysis, detection, and classification of damage in complex bolted structures is an important component of structural health monitoring. In this article, an advanced signal processing and classification method is introduced based on time-frequency techniques. The time-varying signals collected from sensors are decomposed into linear combinations of highly localized Gaussian functions using the matching pursuit decomposition algorithm. These functions are chosen from a dictionary of time-frequency shifted and scaled versions of an elementary Gaussian basis function. The dictionary is also modified to use real measured data as the basis elements in order to obtain a more parsimonious signal representation. Classification is then achieved by matching the extracted damage features in the time-frequency plane. To further improve classification performance, the information collected from multiple sensors is integrated using a Bayesian sensor fusion approach. Results are presented demonstrating the algorithm performance for classifying signals obtained from various types of fastener failure damage in an aluminum plate.
Stable Incorporation of Gold Nanorods into <i>N</i>-Isopropylacrylamide Hydrogels and Their Rapid Shrinkage Induced by Near-Infrared Laser Irradiation (2007)

In this study, we prepared gold nanorod (NR)-embedded N-isopropylacrylamide (NIPAM) hydrogels and studied their volume phase transition behavior induced by near-infrared (near-IR) laser irradiation utilizing the photothermal conversion characteristics of the NRs. When poly(ethylene glycol)-modified NRs were used for the preparation of composite gels, the NRs showed marked dispersion stability in the gel. Near-IR laser irradiation of the gel (cylindrical shape, diameter = 140 microm) under the following conditions, NR concentrations in the gel > or =100 microM and laser irradiation power > or =490 mW, resulted in shrinkage of the gel in the following manner: (1) waist formation around the irradiation spot and (2) growth of the waist along the axial directions of the gel. The gel shrinking induced by near-IR irradiation occurred much more rapidly than that afforded by a temperature jump, because the former was not accompanied by the skin layer formation, which disturbs the rapid shrinking of the gels. When a composite gel containing the model drug (rhodamine-labeled dextran) was irradiated with a near-IR laser, the rapid release of the drug was observed. Taking advantage of the high spatial resolution of the irradiation point, we further achieved the irradiation-point-specific release of the drug from one such gel.
Generalized Fluid System Simulation Program (GFSSP) - Version 6 (2015)

The Generalized Fluid System Simulation Program (GFSSP) is a finite-volume based general-purpose computer program for analyzing steady state and time-dependent flow rates, pressures, temperatures, and concentrations in a complex flow network. The program is capable of modeling real fluids with phase changes, compressibility, mixture thermodynamics, conjugate heat transfer between solid and fluid, fluid transients, pumps, compressors, flow control valves and external body forces such as gravity and centrifugal. The thermo-fluid system to be analyzed is discretized into nodes, branches, and conductors. The scalar properties such as pressure, temperature, and concentrations are calculated at nodes. Mass flow rates and heat transfer rates are computed in branches and conductors. The graphical user interface allows users to build their models using the 'point, drag, and click' method; the users can also run their models and post-process the results in the same environment. The integrated fluid library supplies thermodynamic and thermo-physical properties of 36 fluids, and 24 different resistance/source options are provided for modeling momentum sources or sinks in the branches. Users can introduce new physics, non-linear and time-dependent boundary conditions through user-subroutine.
Clinical Removal of Iron (1982)

Postpartum depression (PPD) is common, disabling, and treatable. The strongest risk factor is a history of mood or anxiety disorder, especially having active symptoms during pregnancy. As PPD is one of the most common complications of childbirth, it is ...Read More
Genetic and Pharmacological Inhibition of PDK1 in Cancer Cells (2010)

Phosphoinositide-dependent kinase 1 (PDK1) is a critical activator of multiple prosurvival and oncogenic protein kinases and has garnered considerable interest as an oncology drug target. Despite progress characterizing PDK1 as a therapeutic target, pharmacological support is lacking due to the prevalence of nonspecific inhibitors. Here, we benchmark literature and newly developed inhibitors and conduct parallel genetic and pharmacological queries into PDK1 function in cancer cells. Through kinase selectivity profiling and x-ray crystallographic studies, we identify an exquisitely selective PDK1 inhibitor (compound 7) that uniquely binds to the inactive kinase conformation (DFG-out). In contrast to compounds 1-5, which are classical ATP-competitive kinase inhibitors (DFG-in), compound 7 specifically inhibits cellular PDK1 T-loop phosphorylation (Ser-241), supporting its unique binding mode. Interfering with PDK1 activity has minimal antiproliferative effect on cells growing as plastic-attached monolayer cultures (i.e. standard tissue culture conditions) despite reduced phosphorylation of AKT, RSK, and S6RP. However, selective PDK1 inhibition impairs anchorage-independent growth, invasion, and cancer cell migration. Compound 7 inhibits colony formation in a subset of cancer cell lines (four of 10) and primary xenograft tumor lines (nine of 57). RNAi-mediated knockdown corroborates the PDK1 dependence in cell lines and identifies candidate biomarkers of drug response. In summary, our profiling studies define a uniquely selective and cell-potent PDK1 inhibitor, and the convergence of genetic and pharmacological phenotypes supports a role of PDK1 in tumorigenesis in the context of three-dimensional in vitro culture systems.
Deep Fitting Degree Scoring Network for Monocular 3D Object Detection (2019)

In this paper, we propose to learn a deep fitting degree scoring network for monocular 3D object detection, which aims to score fitting degree between proposals and object conclusively. Different from most existing monocular frameworks which use tight constraint to get 3D location, our approach achieves high-precision localization through measuring the visual fitting degree between the projected 3D proposals and the object. We first regress the dimension and orientation of the object using an anchor-based method so that a suitable 3D proposal can be constructed. We propose FQNet, which can infer the 3D IoU between the 3D proposals and the object solely based on 2D cues. Therefore, during the detection process, we sample a large number of candidates in the 3D space and project these 3D bounding boxes on 2D image individually. The best candidate can be picked out by simply exploring the spatial overlap between proposals and the object, in the form of the output 3D IoU score of FQNet. Experiments on the KITTI dataset demonstrate the effectiveness of our framework.
Sarcoidosis Uveitis Mimicking the Presentation of Intraocular Lymphoma (2019)

We aimed to report a case of sarcoidosis presenting with atypical clinical findings that mimicked intraocular lymphoma. A 73-year-old female presented with flashes and decreased vision in her left eye over a 2-week period. The patient had no previous ocular medical history. Initial fundal examination revealed vitritis (vitreous inflammation) with retinal hemorrhage and subretinal yellowish lesions in the left eye. All tests were negative for infectious causes. Serum angiotensin-converting enzyme (ACE) was normal. Flow cytometric analysis of vitreous showed a suspicious CD4 + T lymphoid population missing CD7 surface antigen and an elevated CD4/CD8 ratio. Chest CT showed hilar lymphadenopathy. Clinical findings to this point raised the suspicion of lymphoma. Hilar lymph node biopsy demonstrated granulomatous inflammation, confirming the diagnosis of sarcoidosis. Sarcoidosis presenting with uveitis in an elderly patient may pose a diagnostic challenge due to variable presentation and absence of diagnostic criteria. Retinitis and subretinal infiltrates can be a misleading clinical finding of sarcoidosis masquerading as an intraocular oncologic process such as lymphoma. J Med Cases. 2019;10(1):4-7 doi: https://doi.org/10.14740/jmc3190