gebhart/patrepeval huggingface dataset:
Characterization of alpha- and beta-adrenergic and angiotensin receptors in cultured vascular smooth muscle cells of rat aorta. (1985)

To study the cellular mechanism of vascular responsiveness by vasoactive hormones, such as catecholamines and angiotensin (A), the vascular smooth muscle cells (VSMC) of two clonal cell lines (A7r5 and A10) from rat embryo, and of adult rat aorta were established in culture. Binding studies using 125I-labeled-hydroxyphenylethylaminoethyltetralone as an alpha-adrenergic ligand and 125I-labeled-iodocyanopindolol as a beta-adrenergic ligand, revealed that cultured VSMCs contain both alpha- and beta-adrenergic receptors; the binding was specific, rapid, reversible, and saturable. alpha-Adrenergic receptors appear to be a single class of high-affinity binding sites with an apparent dissociation constant (Kd) of approximately 2 X 10(-10) M and a maximal binding capacity (Bmax) of approximately 300,000-400,000 sites/cell, and exclusively of alpha 1-subtype that is responsible for smooth muscle contraction. On the other hand, beta-adrenergic receptors show almost comparable characteristics with the apparent Kd of approximately 0.7-1.1 X 10(-10) M and Bmax of approximately 50,000-130,000 sites/cell, and consist predominantly of beta 2-subtype that mediates smooth muscle relaxation. Furthermore, beta-adrenergic receptors are coupled to adenylate cyclase system, of which activation by beta-agonists induces intracellular cyclic AMP formation. In contrast, the binding of 125I-labeled-AII was demonstrated only in A7r5. The binding declined rapidly during incubation possibly due to faster degradation of AII by proteolytic enzyme(s). AII receptors appear to be a single class of high-affinity binding sites with the apparent Kd of approximately 0.9 X 10(-10) M and Bmax of approximately 11,000 sites/cell, of which affinity is higher than any of vascular AII receptors previously reported.(ABSTRACT TRUNCATED AT 250 WORDS)
Bis(phosphinimino)methanides as Ligands in the Chemistry of the Rare Earth Elements (2006)

Abstract Rare earth element complexes having the very bulky bis(phosphinimino)methanides ligand, {CH(PPh 2 NSiMe 3 ) 2 } − , in the coordination sphere are reviewed. The coordination chemistry, the reactivity, and the catalytic application of the corresponding complexes are discussed.
Dependency Relations among International Stock Market Indices (2015)

We develop networks of international stock market indices using information and correlation based measures. We use 83 stock market indices of a diversity of countries, as well as their single day lagged values, to probe the correlation and the flow of information from one stock index to another taking into account different operating hours. Additionally, we apply the formalism of partial correlations to build the dependency network of the data, and calculate the partial Transfer Entropy to quantify the indirect influence that indices have on one another. We find that Transfer Entropy is an effective way to quantify the flow of information between indices, and that a high degree of information flow between indices lagged by one day coincides to same day correlation between them.
Anchoring of intratumorally administered cytokines to collagen safely potentiates systemic cancer immunotherapy (2019)

The clinical application of cytokine therapies for cancer treatment remains limited due to severe adverse reactions and insufficient therapeutic effects. Although cytokine localization by intratumoral administration could address both issues, the rapid escape of soluble cytokines from the tumor invariably subverts this effort. We find that intratumoral administration of a cytokine fused to the collagen-binding protein lumican prolongs local retention and markedly reduces systemic exposure. Combining local administration of lumican-cytokine fusions with systemic immunotherapies (tumor-targeting antibody, checkpoint blockade, cancer vaccine, or T cell therapy) improves efficacy without exacerbating toxicity in syngeneic tumor models and the <i>Braf<sup>V600E</sup></i> /<i>Pten<sup>fl/fl</sup></i> genetically engineered melanoma model. Curative abscopal effects on noncytokine-injected tumors were also observed as a result of a protective and systemic CD8<sup>+</sup> T cell response primed by local therapy. Cytokine collagen-anchoring constitutes a facile, tumor-agnostic strategy to safely potentiate otherwise marginally effective systemic immunotherapies.
Dianilides of dipicolinic acid function as synthetic chloride channels (2010)

We report that N(2),N(6)-bis(4-nitrophenyl)pyridine-2,6-dicarboxamide, which is related to known isophthalic acid dianilides, transports Cl(-) ions through phospholipid bilayer membranes and shows clear evidence of channel activity.
Bombyxin F1 Gene: Structure and Expression of a New Bombyxin Family Gene That Forms a Pair with Bombyxin B10 Gene (1997)

Bombyxin F1 gene, a new bombyxin family gene, has been identified. The F1 gene forms a pair with bombyxin B10 gene with an opposite transcriptional orientation and the gene pair F1/B10 is located between bombyxin gene pairs B9/C1 and A7/B7 in a bombyxin gene cluster. The nucleotide sequence of the F1 gene and its deduced amino acid sequence deviate moderately from those characterized previously for the family-A, family-B, family-C, family-D, and family-E bombyxin genes; the bombyxin F1 gene and preprobombyxin F1 share no more than 62% and 53% sequence identities with other bombyxin members, respectively. Harr-plot analysis indicated that the spacer of the F1/B10 gene pair has low sequence similarity with that of other bombyxin gene pairs characterized. The bombyxin F1 mRNA in Bombyx mori brain was shown to locate in four pairs of medial neurosecretory cells, which also produce other bombyxin family mRNAs. Genomic Southern hybridization indicated that the Bombyx haploid genome contains a single copy of the family-F bombyxin gene.
Estimates of Intracellular Dopamine in Parkinson’s Disease: A Systematic Review and Meta-Analysis (2021)

Free, intracellular dopamine levels are not reduced in Parkinson's disease compared to normals to a similar degree as are total tissue concentrations, supporting the relevance of modulating VMAT2, neuromelanin and/or dopamine synthesis as rational neuroprotective strategies.
Fiber in Fiber (FIF) bioartificial liver device: initial design and prototyping (2010)

Computer-Aided Design and Applications is an international journal on the applications of CAD and CAM. It publishes papers in the general domain of CAD plus in emerging fields like bio-CAD, nano-CAD, soft-CAD, garment-CAD, PLM, PDM, CAD data mining, CAD and the internet, CAD education, genetic algorithms and CAD engines. The journal is aimed at all developers and users of CAD technology to ptovide CAD solutions for various stages of design and manufacturing. The journal publishes all about Computer-Aided Design and Computer-Aided technologies.
Improved Intrinsic Gettering Technique for High-Temperature-Treated CZ Silicon Wafers (1981)

An improved intrinsic gettering (IG) technique for silicon wafers subjected to a high temperature of at least 1200°C is developed. It has been shown that multi-step annealing from low to high temperatures successively can enhance the IG effectiveness for high-temperature-annealed wafers. Moreover, this technique produces the IG ability even in a low oxygen wafer, ∼11×10 17 cm -3 .
Software Evaluation and Choice: Predictive Validation of the Technology Acceptance Instrument (1994)

Information technology (IT) researchers repeatedly insist upon the rigorous validation of measuring instruments, yet most IT instruments remain at an early stage of development.The Technology Acceptance Model indicate that perceived usefulness and ease of use will influence an individual's intentios to use an information technology. This research introduces a criterion variable that measures intentions as a choice among sevearl alternatives with subjects actually expecting to use their choice.
Three-dimensional reconstruction methods for micro-rotation fluorescence microscopy (2009)

Micro-rotation fluorescence microscopy is a novel, optical imaging technique developed with a cell rotation system. The imaging system enables individual living cells to be rotated in suspension under microscopic dimensions, and allows us to acquire a series of images of the cells, simultaneously during the rotation. A challenging task in micro-rotation imaging is how to determine three-dimensional (3D) cell structure from the image series. \n This thesis thus presents four alternative methods for reconstructing 3D objects from a series of micro-rotation images. The three former methods, the expectation maximisation (EM), the generalised Skilling-Bryan and the marginalisation methods, are iterative algorithms built on the Bayesian inversion theory, which is used to quantify uncertainties in data and model parameters, and also to utilise prior information about the unknown structure. The fourth method, dual filtered backprojection (DFBP), is a fast, non-iterative algorithm derived from the Fourier slice theorem in the classical computed tomography. Each method has its own features: the EM method serves as a basic tool for general usability; the Skilling-Bryan method is more flexible for modelling of noise and prior; the marginalisation method serves as a statistical treatment of the reconstruction that suffers from inaccurate image alignment; and the DFBP method beats the other methods by computational speed but restricts itself with a certain imaging condition. In general, selection of the reconstruction methods depends on imaging and data conditions, such as conventional widefield or confocal microscopy, the stability of cell rotation, and the quality of image alignment. \n In conclusion, all the proposed reconstruction methods clearly increase capability to visualise 3D object structures, as shown by both simulations and experiments with real micro-rotation data. Two obvious messages from the results are that first, the quality of the reconstructed object highly depends on the accuracy of image alignment, and second, micro-rotation reconstructions with the current imaging system always contain poor resolution in the tangential direction of the rotation. Future interesting research is thus to combine the micro-rotation protocol with extended depth-of-focus microscopy that could strengthen the tangential resolution.
Mild and Selective Reduction of Imines:  Formation of an Unsymmetrical Macrocycle (2004)

During investigations of 5, a [3 + 3] Schiff-base macrocycle with six imines, a partially reduced Schiff-base macrocycle, 6, possessing one CH(2)NH and five imine groups was obtained. Control experiments and deuterium labeling indicate that the macrocycle is reduced by a benzimidazoline generated during the reaction. Benzimidazolines may be convenient reagents for the mild and selective reduction of imines.
A Facile Cu(I)/TF-BiphamPhos-Catalyzed Asymmetric Approach to Unnatural α-Amino Acid Derivatives Containing<i>gem</i>-Bisphosphonates (2011)

A novel catalytic asymmetric Michael addition of azomethine ylide with β-substituted alkylidene bisphosphates was realized in the presence of a chiral copper(I)/TF-BiphamPhos complex. The present system provides a unique and facile access to enantioenriched unnatural α-amino acid derivatives containing gem-bisphosphonates (gem-BPs) in high yields with excellent diastereoselectivities and enantioselectivities. Subsequent transformations lead to the expedient preparation of biologically active unnatural α-amino acid derivatives containing BPs and bisphosphonic acids without loss of diastereo- and enantiomeric excess.
Evaluation of Geriforte, an Herbal Geriatric Tonic, on Antioxidant Defense System in Wistar Rats (1994)

Geriforte, an herbomineral compound prepared from several herbs and minerals, is being used as a restorative tonic to solve the problems of old age in India. Since active oxygen species have been proposed to be involved in the aging process of the brain, alterations in the level of enzymes involved in the defense system against free radicals and other active oxygen species could substantially influence the aging process. Therefore, in the present study, this antistress adaptogenic Ayurvedic preparation has been evaluated for the activities of superoxide dismutase (SOD) and catalase (CAT), key enzymes of the cellular defense system, in the brain of aging Wistar male rats. The present study was carried out on 22-month-old rats. They were given Geriforte powder orally at a dosage of 900 mg/kg body weight once a day for two months. The Geriforte-treated rat brain had significantly high levels of SOD and CAT activities than those recorded in their respective controls. The contents of SOD and CAT in Geriforte-administered brain increased by 24% and 30%, respectively. In the present investigation, free radical damage was assessed by measuring thiobarbituric acid reactive substance (TBARS) in postmitochondrial fractions of brain homogenate. The magnitude of TBARS has been reported to be decreased by 26% in the Geriforte-treated rat brain. In conclusion, it seems logical to suppose that Geriforte, a combination of several plant ingredients and minerals, protects the body against the damaging effects of free radicals by producing large amounts of antioxidant enzymes.
Toward an Acoustic Noninvasive Diagnosis of Urinary Bladder Outlet Obstruction (2008)

On average, in aging males, the prostate enlarges (benign prostatic enlargement or BPE) and may cause bladder outlet obstruction (BOO). The internationally standardized method for diagnosing BOO is based on measurements of urinary flow rate and bladder pressure, using a catheter inserted into the bladder via the urethra. This method is invasive, time-consuming, and uncomfortable for the patient. We are developing a novel diagnostic method based on perineal recording of sound during urinary flow. Although it is known that (some aspects of) the recorded sound are (among others) related to the degree of obstruction, an exact and unique relation allowing derivation of the degree of obstruction from the sound recording is not known. In a biophysical model of the urethra, we found that the weighted average frequency, the standard deviation, and the skewness of the power spectrum are monotonically related to the degree of obstruction. The standard deviation was the most significant predictor of BOO (89% correct). Based on this model study, we are confident that a simple noninvasive acoustic method for diagnosing BOO caused by BPE can be developed. This would lower the threshold for urodynamic testing of patients with lower urinary tract symptoms resulting in higher therapeutic efficacy.
Cell Internalization of Magnetic Nanoparticles Using Transfection Agents (2007)

Transfection agent (TFA)-induced magnetic cell labeling with Feridex IV is an attractive method of loading cells because it employs a pharmaceutical source of iron oxide. Although attractive, the method has two significant drawbacks. First, it requires mixing positively charged transfection agents and negatively charged magnetic nanoparticles, and the resulting loss of nanoparticle surface charge causes nanoparticle precipitation. Second, it can result in nanoparticle adsorption to the cell surface rather than internalization. Internalization of Feridex (and associated dextran) is important since dextran cell exterior can react with the antidextran antibodies, commonly present in human populations, and trigger an antibody-mediated cytotoxicity. Here we employed three assays for selecting Feridex/TFA mixtures to minimize nanoparticle precipitation and surface adsorption: (1) an assay for precipitation or stability (light scattering), (2) an assay for labeled cells (percentage of cells retained by a magnetic filter), and (3) an antidextran-based assay for nanoparticle internalization. Cells loaded with Feridex/protamine had internalized iron, whereas cells loaded with Feridex/Lipofectamine had surface-adsorbed iron. Optimal conditions for loading cells were 10 microg/Feridex and 3 microg/mL protamine sulfate. Conditions for loading cells with Feridex and a TFA need to be carefully selected to minimize nanoparticle precipitation and dextran adsorption to the cell surface.
Fluid in Video: Augmenting Real Video with Simulated Fluids (2008)

Abstract We present a technique for coupling simulated fluid phenomena that interact with real dynamic scenes captured as a binocular video sequence. We first process the binocular video sequence to obtain a complete 3D reconstruction of the scene, including velocity information. We use stereo for the visible parts of 3D geometry and surface completion to fill the missing regions. We then perform fluid simulation within a 3D domain that contains the object, enabling one‐way coupling from the video to the fluid. In order to maintain temporal consistency of the reconstructed scene and the animated fluid across frames, we develop a geometry tracking algorithm that combines optic flow and depth information with a novel technique for “velocity completion”. The velocity completion technique uses local rigidity constraints to hypothesize a motion field for the entire 3D shape, which is then used to propagate and filter the reconstructed shape over time. This approach not only generates smoothly varying geometry across time, but also simultaneously provides the necessary boundary conditions for one‐way coupling between the dynamic geometry and the simulated fluid. Finally, we employ a GPU based scheme for rendering the synthetic fluid in the real video, taking refraction and scene texture into account.
Synthesis of stoichiometric nickel aluminate spinel nanoparticles (2015)

Nickel aluminate is a transition metal oxide with spinel structure with potential applications as catalysts and sensors. Both applications benefit from high specific surface areas as well as chemical stoichiometry control. However, a systematic approach to understand synthetic parameters affecting stoichiometry and agglomeration of nickel aluminate nanoparticles is still lacking. In this work, coprecipitation using direct and reverse strikes and polymeric precursor techniques were comparatively studied to address this problem. While the polymeric method could deliver stoichiometric spinel, the samples were highly agglomerated exhibiting low surface area. Both co-precipitation procedures produced smaller sizes and less agglomerated nanoparticles as compared to the polymeric precursor, but for the reverse-strike, Ni2+ preferentially formed a soluble complex with ammonia and led to nickel deficient nanoparticles. Stoichiometric (1 mol NiO:1 mol Al2O3) nanocrystalline nickel aluminate was only achieved when using controlled excess Ni2+. The normal-strike lead to more stoichiometric compositions without need for excess cations, but the obtained nanoparticles were less homogeneous and showed smaller surface areas as compared to the reverse-strike method.
Application of nonresonance excitation to ion trap tandem mass spectrometry and selected ejection chemical ionization (1996)

Nonresonance excitation is a universal ion excitation and ejection method in which increased ion kinetic energy is achieved by the combination of an axial dc dipole and the rf trapping fields. The method does not require the applied excitation frequency to match with the secular frequency of the precursor ions to effect collision-induced dissociation (CID) for tandem mass spectrometry applications. Therefore, it is free of the effects of secular frequency changes caused by space-charge and simplifies the optimization of tandem mass spectrometry parameters when combined with gas chromatography-tandem mass spectrometry (GC-MS/MS). Computer simulations show that in contrast to the resonance excitation process, the nonresonance excitation process is able to accelerate thermal ions to kinetic energies in excess of 40 eV in a few microseconds. Based on simulations, we expect that the rapid deposition of energy by this method may allow the study, in ion traps, of high energy decomposition channels of precursor ions with multiple decomposition channels. Furthermore, the method is able to simultaneously excite multiple precursor ions, for example, excite both analyte and its coeluting isotopically labeled internal standard for GC-MS/MS analysis. A GC-MS/MS analysis of 100 pg of n-butylbenzene is demonstrated with a signal-to-noise ratio of 3624, which is over an order of magnitude higher than the signal-to-noise ratio of 345 obtained by full scan gas chromatography-mass spectrometry. In addition, the nonresonance excitation method can be used as a low pass mass filter in the chemical ionization (CI) mode to eject undesired fragment ions that result from direct electron ionization. This new CI method, selected ejection chemical ionization, can produce a CI spectrum without contamination of sample fragment ions from electron ionization.
Justice prédictive et transhumanisme (2018)

La justice prédictive séduit en ce qu’elle promet une justice plus sûre et meilleure. Pourtant, sa dénomination même, son utilisation, ses symboles, sa légitimité et ses effets méritent d’être critiqués et discutés.
Effect of Airline Crew's Psychological Contract Violation on Organizational Cynicism : Mediating Effect of Job Burnout (2022)

The purpose of this study is to examine the mediating effect of job burnout in the relationship between the psychological contract violation and organizational cynicism of the merged corporate flight attendants after the announcement of the airline M&A. Since M&A causes considerable anxiety and fear in the future of the merged members, we would like to present a human resource management plan for successful M&A by reviewing the impact of negative psychological factors on the company. For this purpose, data were collected by conducting an online survey on flight attendants of airline A, the flight attendants of the merged company, and a total of 316 valid questionnaires were analyzed using SPSS v.23 and AMOS v.23 programs. As a result of the analysis, first, it was confirmed that the perception of psychological contract violation had a positive (+) relationship with organizational cynicism. Second, the perception of psychological contract violation was found to have a positive (+) relationship with job burnout. Third, it was confirmed that job burnout had a significant positive (+) effect on organizational cynicism. Finally, job burnout was found to have a mediating effect on the relationship between psychological contract violation and organizational cynicism. Based on these research results, we would like to present strategic implications for flight attendants human resource management for the currently underway airline M&A merger company.
Creation of a transgenic mouse for hair‐cell gene targeting by using a modified bacterial artificial chromosome containing <i>Prestin</i> (2004)

We made a transgenic mouse that expresses Cre recombinase activity in inner ear hair cells by using a modified bacterial artificial chromosome containing Prestin. Cre recombinase activity was restricted to inner and outer hair cells, a subset of vestibular hair cells, spiral and vestibular ganglia in the inner ear, and a subset of cells in the testis, epididymis, and ear bone. This mouse will be useful for hair-cell-specific gene targeting.