gebhart/patrepeval huggingface dataset:
Identification of the High Affinity Binding Site in Transforming Growth Factor-β Involved in Complex Formation with α2-Macroglobulin (2001)

The biological activities of transforming growth factor-beta isoforms (TGF-beta(1,2)) are known to be modulated by alpha(2)-macroglobulin (alpha(2)M). alpha(2)M forms complexes with numerous growth factors, cytokines, and hormones, including TGF-beta. Identification of the binding sites in TGF-beta isoforms responsible for high affinity interaction with alpha(2)M many unravel the molecular basis of the complex formation. Here we demonstrate that among nine synthetic pentacosapeptides with overlapping amino acid sequences spanning the entire TGF-beta(1) molecule, the peptide (residues 41-65) containing Trp-52 exhibited the most potent activity in inhibiting the formation of complexes between (125)I-TGF-beta(1) and activated alpha(2)M (alpha(2)M*) as determined by nondenaturing polyacrylamide gel electrophoresis and by plasma clearance in mice. TGF-beta(2) peptide containing the homologous sequence and Trp-52 was as active as the TGF-beta(1) peptide, whereas the corresponding TGF-beta(3) peptide lacking Trp-52, was inactive. The replacement of the Trp-52 with alanine abolished the inhibitory activities of these peptides. (125)I-TGF-beta(3), which lacks Trp-52, bound to alpha(2)M* with an affinity lower than that of (125)I-TGF-beta(1). Furthermore, unlabeled TGF-beta(3) and the mutant TGF-beta(1)W52A, in which Trp-52 was replaced with alanine, were less potent than unlabeled TGF-beta(1) in blocking I(125)-TGF-beta(1) binding to alpha(2)M*. TGF-beta(1) and TGF-beta(2) peptides containing Trp-52 were also effective in inhibiting I(125)-nerve growth factor binding to alpha(2)M*. Tauhese results suggest that Trp-52 is involved in high affinity binding of TGF-beta to alpha(2)M*. They also imply that TGF-beta and other growth factors/cytokines/hormones may form complexes with alpha(2)M* via a common mechanism involving the interactions between topologically exposed Trp and/or other hydrophobic residues and a hydrophobic region in alpha(2)M*.
Catalyzed Collapse and Enhanced Hydrogen Storage of BN Nanotubes (2002)

The novel morphology of BN nanotubes with a collapsed structure has been discovered by a metal-catalyzed treatment. The collapse causes the dramatic enlargement of a specific surface area of BN nanotubes and remarkably enhances the hydrogen storage capacity of BN nanotubes.
Assessment of the pharmacodynamic properties of antimalarial drugs in vivo (1997)

Free access1 July 1997 Share on Assessment of the pharmacodynamic properties of antimalarial drugs in vivoAuthor: N J WhiteAuthors Info & AffiliationsDOI: https://doi.org/10.1128/AAC.41.7.1413 PDF/EPUB
Controlled Synthesis of Photomagnetic Nanoparticles of a Prussian Blue Analogue in a Silica Xerogel (2008)

Controlled precipitation: Silica can control the precipitation of a CoFe Prussian Blue analogue (PBA) by reversibly protecting the CoII ions. The precursors are introduced into a silica sol and precipitation of the PBA is triggered by acidification after condensation of the silica network (see picture). This original method provides a homogeneous nanocomposite with important photomagnetic properties. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2008/z802273_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
KSAC, a Defined Leishmania Antigen, plus Adjuvant Protects against the Virulence of L. major Transmitted by Its Natural Vector Phlebotomus duboscqi (2012)

This study demonstrates the protective efficacy of KSAC+GLA-SE against sand fly challenge; the importance of vector-transmitted challenge in evaluating vaccine candidates against Leishmania infection; and the necessity of a rapid potent Th1 response against Leishmania to attain true protection.
Bi-stable Concepts for Reconfigurable Structures (2004)

This study is concerned with novel bi-stable structures, which are proposed for enabling reconfigurable structures with multiple, load-free equilibrium states. The first structure is a discrete member bi-stable module and is deformable in shear. The degree of shape change is predicted by a compatibility analysis. The stiffness of the module in either state is confirmed by a modified version of Maxwell’s Rule for planar frameworks. The stiffness and degree of stability of larger grid-like structures formed by interconnecting modules is also considered. The second structure is a shallow, continuous dome pressed into a thin sheet, which can be inverted by snapping through the sheet. Multiply stable shells are formed by stamping many domes in an ordered array into the complete sheet. It is shown that residual stresses are responsible for the novel, large and stable deformations, and a finite element analysis is performed to investigate the effects of the forming process upon the inversion behaviour of a single dome.
Purification and immunogenicity of fusion VP1 protein of foot and mouth disease virus (1984)

A procedure has been developed to purify foot and mouth disease virus (FMDV) VP1 surface antigens from recombinant Escherichia coli. The VP1 antigens are expressed as fusion proteins derived from the E. coli Trp operon and VP1 surface protein of FMDV. The procedure is capable of recovering greater than 96% of the desired product at a purity of greater than 96%. The resulting antigens induce significant levels of virus-neutralizing antibody in guinea pigs and cattle as determined by a mouse protection assay [Skinner, H.H. (1952) Proc. Int. Vet. Congr., 15th 1, 195]. E. coli contaminants have a deleterious effect on ion-exchange chromatography as well as immunogenicity of the expressed fusion VP1 antigens. The method presented removes significant E. coli contaminants, yielding fusion VP1 proteins which are immunogenically potent. In particular, virus neutralization titers at 100-micrograms dosage of the fusion VP1 proteins of the O1 and A24 serotypes are similar to that induced by the natural VP1 proteins isolated from FMD virions.
Use of Adsorbents for Recovery of Acetic Acid from Aqueous Solutions Part III—Solvent Regeneration (1987)

Abstract Solvent leaching was investigated as a method of regeneration for activated carbons and polymer sorbents loaded with acetic acid by contact with aqueous solutions. Both batch-equilibration and fixed-bed experiments were employed. Methanol, methyl acetate, and acetone are suitable regeneration solvents for most activated carbons. Amoco GX-031 is an exception, giving incomplete regeneration. Methanol provides still more efficient regeneration for macroreticular styrene-divinylbenzenes adsorbents. Among basic sorbents, amine resins give incomplete regeneration by solvent leaching, as does a poly N oxide. Polybenzimidazole, a weaker base, gives higher recoveries of acetic acid when leached by solvents. Poly(4-vinylpyridine) is regenerated completely and efficiently by methanol, methyl acetate, and acetone in a fixed-bed geometry. It thus appears that an intermediate degree of basicity is desirable in a synthetic sorbent for acetic acid – strong enough to give the uptake benefits of acid-base interactions, but weak enough to be regenerable.
Effect of <i>Pseudomonas</i> Infection on Weight Loss, Lung Mechanics, and Cytokines in Mice (2000)

Poor growth, Pseudomonas aeruginosa endobronchitis, pulmonary inflammation, and decline of lung function are hallmarks of cystic fibrosis (CF), yet the relationship between these features is poorly understood. Because animal models of chronic bronchopulmonary infection with P. aeruginosa used to study pulmonary inflammation in CF have also been associated with weight loss, we sought to determine whether this weight loss was due to the inflammatory process and/or to changes in lung function. P. aeruginosa-laden agarose beads were instilled into the lungs of mice. Weight loss was greatest 3 d after Pseudomonas infection. Infected mice had a rapid though transient rise in absolute neutrophil counts, mTNF-alpha, mIL-1beta, mIL-6, mip-2, and KC in bronchoalveolar lavage fluid. There was no difference in lung resistance or lung compliance measured by body plethysmography between infected and control mice. Weight loss did correlate with the concentration of proinflammatory cytokine levels 3 d after inoculation of mice with Pseudomonas, and body composition analysis revealed loss of skeletal muscle mass. These results suggest that weight loss in P. aeruginosa-infected mice was associated with the inflammatory process and not with altered pulmonary responsiveness. These findings may provide insights into the cause of cachexia and weight loss seen in patients with CF.
Enhanced photocurrent in thin-film amorphous silicon solar cells via shape controlled three-dimensional nanostructures (2012)

In this paper, we have explored manufacturable approaches to sub-wavelength controlled three-dimensional (3D) nano-patterns with the goal of significantly enhancing the photocurrent in amorphous silicon solar cells. Here we demonstrate efficiency enhancement of about 50% over typical flat a-Si thin-film solar cells, and report an enhancement of 20% in optical absorption over Asahi textured glass by fabricating sub-wavelength nano-patterned a-Si on glass substrates. External quantum efficiency showed superior results for the 3D nano-patterned thin-film solar cells due to enhancement of broadband optical absorption. The results further indicate that this enhanced light trapping is achieved with minimal parasitic absorption losses in the deposited transparent conductive oxide for the nano-patterned substrate thin-film amorphous silicon solar cell configuration. Optical simulations are in good agreement with experimental results, and also show a significant enhancement in optical absorption, quantum efficiency and photocurrent.
Antioxidant activity of olive phenols: mechanistic investigation and characterization of oxidation products by mass spectrometry (2004)

In this work, the antioxidant activity of olive phenols is first characterized by their stoichiometries n(tot)(number of radicals trapped per antioxidant molecule) and their rate constants for the first H-atom abstraction k(1) by the stable radical DPPH. It appears that oleuropein, hydroxytyrosol and caffeic acid have the largest k(1) values, whereas dihydrocaffeic acid, an intestinal metabolite of caffeic acid, is the best antioxidant in terms of n(tot). For phenols with a catechol moiety n(tot) is higher than two, implying an antioxidant effect of their primarily formed oxidation products. A HPLC-MS analysis of the main products formed in the AAPH-induced oxidation of olive phenols reveals the presence of dimers and trimers. With hydroxytyrosol and dihydrocaffeic acid, oligomerization can take place with the addition of water molecules.The antioxidant activity of olive phenols is then evaluated by their ability to inhibit the AAPH-induced peroxidation of linoleic acid in SDS micelles. It is shown that olive phenols and quercetin act as retardants rather than chain breakers like alpha-tocopherol. From a detailed mechanistic investigation, it appears that the inhibition of lipid peroxidation by olive phenols can be satisfactorily interpreted by assuming that they essentially reduce the AAPH-derived initiating radicals. Overall, olive phenols prove to be efficient scavengers of hydrophilic peroxyl radicals with a long lasting antioxidant effect owing to the residual activity of some of their oxidation products.
Fontolizumab in moderate to severe Crohnʼs disease: A phase 2, randomized, double-blind, placebo-controlled, multiple-dose study (2009)

Although a strong clinical response to fontolizumab was not observed, significant decreases in C-reactive protein levels suggest a biological effect. Fontolizumab was well tolerated, and further studies to assess its efficacy are warranted.
Laser and camera for road edge and mid-line detection (2002)

Presents a methodology for extracting road edge and lane information for smart and intelligent navigation of vehicles. The range information provided by a fast 2D laser range-measuring device is processed by an extended Kalman filter to extract the road edge or curb information. The resultant road edge information is used to aid in the extraction of the mid-line from a CCD camera image using an extended Kalman filter. Experimental results are presented to demonstrate the effectiveness of the combined laser and vision strategy for road-edge and lane detection.
Immune Responses of the Bovine Fetus (1977)

The bovine fetus is capable of mounting an antibody response when a bacterial antigen (killed Escherichia coli) or viral antigen (live reovirus) is deposited into the amniotic fluid. Time required for the fetus to respond to bacterial antigen given orally (amniotic fluid) is approximately 10 to 14 days and 8 to 10 days for viral antigen. Calves vaccinated prenatally with E. coli from 9 to 102 days before birth and deprived of colostrum survived oral challenge doses of viable E. coli which killed calves not vaccinated prenatally. One mechanism of protection was the local production of antibody in the gastrointestinal mucosa where immunofluorescent techniques showed immunoglobulins IgG, IgM, and anti-E. coli antibody in the duodenum, jejunum, and ileum as well as in the jejunal lymph node. Prenatal vaccination has been used in the field for prevention of colibacillosis. However, the occurrence of some stillbirths and premature births indicates the need for further research before there can be widespread field application of the technique.
Oven controlled FBAR oscillator (2015)

Recent work with chip scale integrated FBAR oscillators has highlighted the fact that the temperature sensitivity is similar to SAW resonators. This sensitivity is significantly higher than quartz crystal oscillators, and limits the application of the technology. This investigation looks at the feasibility of stabilizing the frequency of the oscillator with a heater resistor and sensor resistor integrated into close proximity with the resonator, and an on-chip temperature feedback network.
Snake-Based Segmentation of Teeth from Virtual Dental Casts (2010)

Computer-Aided Design and Applications is an international journal on the applications of CAD and CAM. It publishes papers in the general domain of CAD plus in emerging fields like bio-CAD, nano-CAD, soft-CAD, garment-CAD, PLM, PDM, CAD data mining, CAD and the internet, CAD education, genetic algorithms and CAD engines. The journal is aimed at all developers and users of CAD technology to ptovide CAD solutions for various stages of design and manufacturing. The journal publishes all about Computer-Aided Design and Computer-Aided technologies.
Ammonia-regulated expression of a soybean gene encoding cytosolic glutamine synthetase in transgenic Lotus corniculatus. (1991)

A full-length cDNA clone encoding cytosolic glutamine synthetase (GS), expressed in roots and root nodules of soybean, was isolated by direct complementation of an Escherichia coli gln A- mutant. This sequence is induced in roots by the availability of ammonia. A 3.5-kilobase promoter fragment of a genomic clone (lambda GS15) corresponding to this cDNA was isolated and fused with a reporter [beta-glucuronidase (GUS)] gene. The GS-GUS fusion was introduced into a legume (Lotus corniculatus) and a nonlegume (tobacco) plant by way of Agrobacterium-mediated transformations. This chimeric gene was found to be expressed in a root-specific manner in both tobacco and L. corniculatus, the expression being restricted to the growing root apices and the vascular bundles of the mature root. Treatment with ammonia increased the expression of this chimeric gene in the legume background (i.e., L. corniculatus); however, no induction was observed in tobacco roots. Histochemical localization of GUS activity in ammonia-treated transgenic L. corniculatus roots showed a uniform distribution across all cell types. These data suggest that the tissue specificity of the soybean cytosolic GS gene is conserved in both tobacco and L. corniculatus; however, in the latter case, this gene is ammonia inducible. Furthermore, the ammonia-enhanced GS gene expression in L. corniculatus is due to an increase in transcription. That this gene is directly regulated by externally supplied or symbiotically fixed nitrogen is also evident from the expression of GS-GUS in the infection zone, including the uninfected cells, and the inner cortex of transgenic L. corniculatus nodules, where a flux of ammonia is encountered by this tissue. The lack of expression of GS-GUS in the outer cortex of the nodules suggests that ammonia may not be able to diffuse outside the endodermis.
Quinazoline‐4‐piperidine sulfamides are specific inhibitors of human <scp>NPP</scp>1 and prevent pathological mineralization of valve interstitial cells (2015)

We have provided evidence that QPS1 is a potent and selective non-competitive inhibitor of NPP1 and that it prevented pathological mineralization in a cellular model.
Understanding of the first arrivals in the shape of a Christmas tree (2013)

In land seismic data processing, the first arrivals are often picked to image the near surface by applying a traveltime inversion method for statics corrections. In many areas, however, the first arrivals of a shot gather exhibit a pattern like a Christmas tree: the first arrivals quickly disappear over offset and then another branch nearly parallel to the previous one appears with a constant time shift, and the pattern may be repeated further. An initial thought is that these Christmas tree branches are the refractions associated with different layers and imbedded low velocity layers in the near surface cause the delay. To confirm our understanding on such shot gathers, we establish a few numerical models and apply a 1-D elastic modeling program to simulate seismic data. We found that the first refraction quickly fading away because of its propagation through a thin high-velocity layer. The later Christmas tree branches are the wide-angle reflection and its multiples from a low velocity zone right below the thin high-velocity layer. Except for the shallow direct wave and the first refraction branch, all later Christmas tree branches at long offset are not really the “first arrivals.” Numerical experiment indicates that using the traveltimes of these Christmas tree branches for the first arrival traveltime inversion produces false near surface models. For this type of data, a more sophisticated method such as waveform inversion should be applied for near surface imaging.
Marke's disease herpesviruses. III. Purification and characterization of Marek's disease herpesvirus B antigen (1978)

Sera from chickens naturally infected with Marek's disease herpesvirus (MDHV) form preciptin lines with at least two immunologically distinct soluble antigens designated MDHV-A and MDHV-B. Partial purification and characterization of the glycoprotein MDHV-A antigen was previously reported. MDHV-B was found predominantly in the sonically treated extracts of infected cells, in contrast to the predominantly extracellular MDHV-A. Analysis of these extracts from [14C]glucosamine-labeled cells by immunodiffusion with chicken anti MDHV-B serum negative for MDHV-A followed by autoradiography confirmed that MDHV-B was a common antigen between MDHV and herpesvirus of turkeys and revealed that it was also a glycoprotein. Because of their glycoprotein nature, both MDHV-A and MDHV-B bound to concanavalin A affinity chromatography columns and could then be eluted by alpha-methyl-D-mannoside and recovered for further analysis. Concanavalin A affinity chromatography was an excellent technique for initial purification of MDHV-A and MDHV-B, since approximately 5- and 15- fold purification, respectively, was achieved in a single simple step. MDHV-B was resistant to trypsin under conditions where MDHV-A was sensitive, but was similar to MDHV-A in resistance to pH 2.0 and to 1.0 or 2.0 M urea and 0.05% Brij 35. Partially purified MDHV-B was analyzed by sucrose gradient sedimentation, isoelectric focusing, and gel filtration on Sephadex G-200 in the presence of 1.0 or 2.0 M urea and 0.05% Brij 35 to purify the antigen and to determine its physical and chemical properties in comparison with those already reported for MDHV-A. MDHV-B had a much lower isoelectric point in pH 4,54, a higher sedimentation coefficient of 4.4S, and a greater molecular weight of 58,250. These data indicate that MDHV-B is physically distinct from MDHV-A antigen, although the size difference is not sufficient to allow for effective separation. In contrast, the isoelectric point difference of greater than 2 pH units makes isoelectric focusing an effective means of purifying the antigens free of one another. The four-step purification procedure achieved greater than 200-fold purification of MDHV-B. Immunization of rabbits with this highly purified antigen results in the preparation of antisera that appeared monospecific for MDHV-B in immunodiffusion.
Single site polymerization of ethylene and 1‐olefins initiated by rare earth metal complexes (1996)

Abstract Ethylene polymerization was explored by using the racemic SiMe 2 (2‐SiMe 3 ‐4‐tBuC 5 H 2 ) 2 Sm(THF) 2 complex ( 1 ), meso type Me 2 Si(Me 2 SiOSiMe 2 )(3‐tBuC 5 H 2 ) 2 Sm‐(THF) 2 complex ( 2 ), and C 1 ‐symmetric SiMe 2 [2,4‐(SiMe 3 ) 2 C 5 H 2 ][3,4‐(SiMe 3 ) 2 C 5 H 2 ]‐Sm(THF) 2 complex ( 3 ) in the absence of methylaluminoxane. As a result, the meso isomer exhibits highest initiating activity, but the number‐average molecular weight is relatively low ( M n = 50000). In contrast to these complexes, the C 1 ‐symmetric complex provides the highest molecular weight of polyethylene, M n > 1000000, with relatively narrow molecular weight distribution ( M w / M n = 1.60). Only the racemic complex exhibits good activity for the polymerization of propylene, 1‐pentene and 1‐hexene.
Automated and Secure Onboarding for System of Systems (2021)

The Internet of Things (IoT) is rapidly changing the number of connected devices and the way they interact with each other. This increases the need for an automated and secure onboarding procedure for IoT devices, systems and services. Device manufacturers are entering the market with internet connected devices, ranging from small sensors to production devices, which are subject of security threats specific to IoT. The onboarding procedure is required to introduce a new device in a System of Systems (SoS) without compromising the already onboarded devices and the underlying infrastructure. Onboarding is the process of providing access to the network and registering the components for the first time in an IoT/SoS framework, thus creating a chain of trust from the hardware device to its hosted software systems and their provided services. The large number and diversity of device hardware, software systems and running services raises the challenge to establish a generic onboarding procedure. In this paper, we present an automated and secure onboarding procedure for SoS. We have implemented the onboarding procedure in the Eclipse Arrowhead framework. However, it can be easily adapted for other IoT/SoS frameworks that are based on Service-oriented Architecture (SoA) principles. The automated onboarding procedure ensures a secure and trusted communication between the new IoT devices and the Eclipse Arrowhead framework. We show its application in a smart charging use case and perform a security assessment.