gebhart/patrepeval huggingface dataset:
Two-photon vibrational spectroscopy for biosciences based on surface-enhanced hyper-Raman scattering (2006)

Two-photon excitation is gaining rapidly in interest and significance in spectroscopy and microscopy. Here we introduce a new approach that suggests versatile optical labels suitable for both one- and two-photon excitation and also two-photon-excited ultrasensitive, nondestructive chemical probing. The underlying spectroscopic effect is the incoherent inelastic scattering of two photons on the vibrational quantum states called hyper-Raman scattering (HRS). The rather weak effect can be strengthened greatly if HRS takes place in the local optical fields of gold and silver nanostructures. This so-called surface-enhanced HRS (SEHRS) is the two-photon analogue to surface-enhanced Raman scattering (SERS). SEHRS provides structurally sensitive vibrational information complementary to those obtained by SERS. SEHRS combines the advantages of two-photon spectroscopy with the structural information of vibrational spectroscopy and the high-sensitivity and nanometer-scale local confinement of plasmonics-based spectroscopy. We infer effective two-photon cross-sections for SEHRS on the order of 10(-46) to 10(-45) cm4 x s, similar to or higher than the best "action" cross-sections (product of the two-photon absorption cross-section and fluorescence quantum yield) for two-photon fluorescence, and we demonstrate HRS on biological structures such as single cells after incubation with gold nanoparticles.
Identification of two testosterone-responsive testicular proteins in Sertoli cell-enriched culture medium whose secretion is suppressed by cells of the intact seminiferous tubule. (1987)

Of 30 proteins identified in medium from primary Sertoli cell-enriched cultures, five of these proteins appear to increase primarily in response to testosterone. Using high performance liquid chromatography, two of these proteins, designated CMB-22 and CMB-23, were purified to apparent homogeneity from medium. Both are monomeric proteins with apparent molecular weights of Mr 37,000 and Mr 40,000, respectively. Both interact with concanavalin A and wheat germ agglutinin on lectin blots. CMB-22 has a pI of 5.8; CMB-23 has two distinctive isoelectric variants with pIs of 5.4 and 5.2, the latter variant was designated CMB-23 Isoform. Polyvalent antisera raised against purified CMB-22 and CMB-23 in rabbits cross-reacted with one another. Removal of carbohydrate from these proteins by either enzymatic or chemical treatments reduced their apparent molecular weights but did not abolish their size differences on sodium dodecyl sulfate-polyacrylamide gels. Peptide maps generated by Staphylococcus aureus protease V8 and visualized by silver staining and immunoblots suggest that CMB-22 and CMB-23 are similar but distinctive proteins that share almost identical epitopes. A radioimmunoassay was developed and used to measure total immunoreactive CMB-22-like material (CMB-22 plus CMB-23 immunoreactivity) in culture media, biological fluids, and tissue extracts. The results of these studies showed that the secretion of CMB-22-like material is unique with regard to other proteins that have been identified in Sertoli cell-enriched cultures. That is, CMB-22-like material is secreted by Sertoli cell-enriched cultures, but cannot be detected in media from cultures of intact tubular segments in vitro. In addition, immunoreactive material is also not detected in the testicular fluids from interstitium, tubule, or rete testis. This is in striking contrast to other Sertoli cell proteins which are present in substantial concentrations in these fluids. These observations suggest that the secretion and possibly the hormone responsiveness of CMB-22 and CMB-23 are normally suppressed in the intact tubule both in vivo and in vitro. We propose that studies of CMB-22 and CMB-23 will provide important insights into cell-cell interactions in the seminiferous epithelium.
Efficient Detection of Exact Redundancies in Audio Signals (2008)

The papers at this Convention have been selected on the basis of a submitted abstract and extended precis that have been peer reviewed by at least two qualified anonymous reviewers. This convention paper has been reproduced from the author&apos;s advance manuscript, without editing, corrections, or consideration by the Review Board. The AES takes no responsibility for the contents.
Osmotic Tolerance of Rabbit and Human Corneal Endothelium (1981)

Rabbit and human corneas were mounted in a specular microscope and perfused with a balanced salt solution of varying osmolality (200 to 500 mOsm). Measurements of corneal thickness were made throughout the perfusion period, and at selected times the corneas were fixed and prepared for scanning and transmission electron microscopy. A hypo-osmotic perfusion medium caused an increase in corneal thickness; by comparison, a hyperosmotic perfusion medium decreased corneal thickness in both rabbit and human corneas. Despite the marked changes in corneal thickness and the water movement that occurred across the endothelium, the cellular ultrastructure remained intact. In reversal studies (return to 300-mOsm perfusion medium), corneal thickness returned to control values with no marked changes in endothelial cell structure. These data indicate that the corneal endotheium can tolerate a wide range of solution osmolalities (200 to 400 mOsm) without marked endotheial cell breakdown if the essential ions are present.
Location-based pairwise key establishments for static sensor networks (2003)

Sensor networks are ideal candidates for applications such as target tracking and environment monitoring. Security in sensor networks is critical when there are potential adversaries. Establishment of pairwise keys is a fundamental security service, which forms the basis of other security services such as authentication and encryption. However, establishing pairwise keys in sensor networks is not a trivial task, particularly due to the resource constraints on sensors. This paper presents several techniques for establishing pairwise keys in static sensor networks. These techniques take advantage of the observation that in static sensor networks, although it is difficult to precisely pinpoint sensors' positions, it is often possible to approximately determine their locations. This paper presents a simple location-aware deployment model, and develops two pairwise key predistribution schemes, a closest pairwise keys predistribution scheme and a location-based pairwise keys scheme using bivariate polynomials, by taking advantage of sensors' expected locations. The analysis in this paper indicates that these schemes can achieve better performance if such location information is available and that the smaller the deployment error (i.e., the difference between a sensor's actual location and its expected location) is, the better performance they can achieve.
Syndecan‐1 limits the progression of liver injury and promotes liver repair in acetaminophen‐induced liver injury in mice (2017)

These results demonstrate that 2-O-sulfated domains in syndecan-1 HS halt disease progression and promote liver repair by enhancing hepatocyte survival in AILI. We propose that syndecan-1 is a critical endogenous factor that controls the balance between prosurvival signaling and apoptosis in hepatocytes in APAP liver disease. (Hepatology 2017;66:1601-1615).
Selective CO<sub>2</sub> Separation from CO<sub>2</sub>−N<sub>2</sub> Mixtures by Immobilized Carbonate−Glycerol Membranes (1999)

Separation of carbon dioxide from a humid mixture of CO2−N2 through membranes containing immobilized solutions of Na2CO3−glycerol in porous and hydrophilic poly(vinylidene fluoride) (PVDF) substrate was experimentally studied for use as a venting membrane in space-walk applications. The effects of Na2CO3 concentration, CO2 partial pressure, and feed stream relative humidity (RH) were investigated. The carbonate concentration was in the range of 0−4.0 mol/dm3. The feed gas RH range was 49−100%; the sweep gas was dry helium. CO2 partial pressure (pCO2,f) range was 0.007−0.77 atm. Addition of Na2CO3 increased the CO2 permeability drastically at lower carbonate concentrations; at higher Na2CO3 concentrations, this permeability increase is partly compromised by increased solution viscosity and salting-out effect. N2 permeability coefficient decreased with an increase in Na2CO3 concentration. Very high CO2/N2 selectivities were observed at high Na2CO3 concentrations. Higher CO2/N2 selectivities were observed at lower CO2 partial pressure differentials. Steady-state water content in the hygroscopic immobilized liquid membrane (ILM) increases with an increase in feed stream RH. The water content in the ILM considerably affects its viscosity and the effective concentration of the carriers in the ILM; those factors determine the permeation performances of the ILM. Generally, lower permeances and greater CO2/N2 selectivity values were observed at lower feed stream RHs. When the feed RH = 50.7%, pCO2,f = 0.007 atm and the Na2CO3 concentration was 1.0 mol/dm3; the separation factor α(CO2/N2) observed was 3440. Prolonged runs lasting 14 days showed that the ILM permeation performances were quite stable. The ILMs were also found to be stable when challenged with feed streams of very low RHs.
Constitutive activation of FLT3 in acute myeloid leukaemia and its consequences for growth of 32D cells (2000)

The receptor tyrosine kinase Flt3 is expressed on leukaemic blasts of most cases with acute myeloid leukaemia (AML). In order to evaluate the presence and significance of constitutive activation of Flt3 for leukaemogenesis, we (1) analysed the expression and activation status of the receptor in AML blasts; and (2) evaluated the functional consequences of constitutively active Flt3 in a myeloid progenitor cell line. Immunoprecipitation studies revealed Flt3 expression in a high proportion of AML cases (27/32) with ligand-dependent Flt3 autophosphorylation in 18, constitutive autophosphorylation in three and no autophosphorylation in six cases. Only one out of three samples with constitutively active Flt3 but 3/18 samples with ligand-dependent autophosphorylated Flt3 contained the recently described internal tandem repeat (ITR) mutations. To test the significance of Flt3 activation in myeloid cell function, we also characterized the biochemical and biological effects of the activating mutation D838V of Flt3 (FLt3D838V) on the factor-dependent myeloid progenitor cell line 32Dcl3: cells transfected with wild-type Flt3 (32D/Flt3) grew FLt3 ligand (FL) dependent, and the receptor was ligand dependently autophosphorylated. In contrast, the receptor was constitutively autophosphorylated in 32D/Flt3D838V cells, which grew independently of FL. We conclude that, in some AML samples, Flt3 is constitutively activated and that this does not correlate with ITR mutations in the juxtamembrane domain. Furthermore, constitutively active Flt3 confers factor independence to the myeloid progenitor cell line 32D. It remains to be determined whether activation of Flt3 is leukaemogenic in vivo and whether strategies aimed at inhibition of Flt3 activation could inhibit leukaemogenesis.
“Flash” Synthesis of CdSe/CdS Core–Shell Quantum Dots (2013)

We report on the “flash” synthesis of CdSe/CdS core–shell quantum dots (QDs). This new method, based on a seeded growth approach and using an excess of a carboxylic acid, leads to an isotropic and epitaxial growth of a CdS shell on a wurtzite CdSe core. The method is particularly fast and efficient, allowing the controllable growth of very thick CdS shells (up to 6.7 nm in the present study) in no more than 3 min, which is considerably shorter than in previously reported methods. The prepared materials present state-of-the-art properties with narrow emission and high photoluminescence quantum yields, even for thick CdS shells. Additionally, Raman analyses point to an alloyed interface between the core and the shell, which, in conjunction with the thickness of the CdS shell, results in the observed considerable reduction of the blinking rate.
A 30mW 8b 200MS/s pipelined CMOS ADC using a switched-opamp technique (2005)

An 8b 200MS/s 2.8b-per-stage pipelined ADC is realized in a 0.18/spl mu/m CMOS process. By using partially switched operational amplifiers, the ADC consumes 30mW from a 1.8V supply and occupies 0.15mm/sup 2/. The ADC achieves 47.3dB SNDR, 55.8dB SFDR, and 7.6 ENOB for a 90MHz input at 200MS/s.
A theory of noise in GaAs FET microwave oscillators and its experimental verification (1983)

A theory has been developed which provides an entirely analytical approach to the calculation of AM and FM noise in free-running GaAs FET microwave oscillators. The theory is based on the model that low-frequency device noise is mixed with the carrier signal via the nonlinearity of the FET and upconverted to microwave frequencies. Because of the analytical nature of the theory, all the important device and circuit parameters on which the noise generation depends are explicitly given. Two GaAs FET oscillators have been fabricated and used to investigate the FM noise. The theory predicts well both the spectral dependence and the absolute magnitude of the FM noise in both oscillators. The noise performance of the oscillators differs by 19 dB. The theory indicates that no single factor is responsible for this, and moreover that attention should be given to the optimization of many device and circuit features in the design of a low noise FET oscillator.
Microwave Processing of Materials and Applications in Manufacturing Industries: A Review (2014)

The main focused aim of developing new processing and manufacturing technologies are to reduce production or manufacturing costs, processing times, and to enhance manufactured product properties. The developed processing techniques should be widely acceptable for all types of materials including metal matrix composites, ceramics, alloys, and fiber reinforced plastics. Microwave materials processing is emerging as a novel processing technology which is applicable to a wide variety of materials system including processing of MMC, FRP, alloys, ceramics, metals, powder metallurgy, material joining, coatings, and claddings. In comparison to the conventional processes, microwave processing of materials offers better mechanical properties with reduced defects and economical advantages in terms of power and time savings. The present review work focuses mainly on global developments taking place in the field of microwave processing of materials and their relevant industrial applications.
Teaching the testicular exam: a model curriculum from "A" to "Zack". (2004)

To address curricular deficits, a self-contained module on the testicular exam has been successfully incorporated into family medicine clerkships at two different medical schools. This module is easily adaptable to other settings and institutions.
Enterotype May Drive the Dietary-Associated Cardiometabolic Risk Factors (2017)

Analyses of typical bacterial clusters in humans named enterotypes may facilitate understanding the host differences in the cardiometabolic profile. It stills unknown whether the three previously described enterotypes were present in populations living below the equator. We examined how the identification of enterotypes could be useful to explain the dietary associations with cardiometabolic risk factors in Brazilian subjects. In this cross-sectional study, a convenience sample of 268 adults (54.2% women) reported their dietary habits and had clinical and biological samples collected. In this study, we analyzed biochemical data and metagenomics of fecal microbiota (16SrRNA sequencing, V4 region). Continuous variables were compared using ANOVA, and categorical variables using chi-square test. Vsearch clustered the operational taxonomic units, and Silva Database provided the taxonomic signatures. Spearman coefficient was used to verify the correlation between bacteria abundances within each enterotype. One hundred subjects were classified as omnivore, 102 lacto-ovo-vegetarians, and 66 strict vegetarians. We found the same structure as the three previously described enterotypes: 111 participants were assigned to <i>Bacteroides</i>, 55 to <i>Prevotella</i>, and 102 to <i>Ruminococcaceae</i> enterotype. The <i>Prevotella</i> cluster contained higher amount of strict vegetarians individuals than the other enterotypes (40.0 vs. 20.7 and 20.6, <i>p</i> = 0.04). Subjects in this enterotype had a similar anthropometric profile but a lower mean LDL-c concentration than the <i>Bacteroides</i> enterotype (96 ± 23 vs. 109 ± 32 mg/dL, <i>p</i> = 0.04). We observed significant correlations between bacterial abundances and cardiometabolic risk factors, but coefficients differed depending on the enterotype. In <i>Prevotella</i> enterotype, <i>Eubacterium ventriosum</i> (r BMI = -0.33, <i>p</i> = 0.03, and r HDL-c = 0.33, <i>p</i> = 0.04), <i>Akkermansia</i> (r 2h glucose = -0.35, <i>p</i> = 0.02), <i>Roseburia</i> (r BMI = -0.36, <i>p</i> = 0.02 and r waist = -0.36, <i>p</i> = 0.02), and <i>Faecalibacterium</i> (r insulin = -0.35, <i>p</i> = 0.02) abundances were associated to better cardiometabolic profile. The three enterotypes previously described are present in Brazilians, supporting that those bacterial clusters are not population-specific. Diet-independent lower LDL-c levels in subjects from <i>Prevotella</i> than in other enterotypes suggest that a protective bacterial cluster in the former should be driving this association. Enterotypes seem to be useful to understand the impact of daily diet exposure on cardiometabolic risk factors. Prospective studies are needed to confirm their utility for predicting phenotypes in humans.
Molecular definition of distinct cytoskeletal structures involved in complement- and Fc receptor-mediated phagocytosis in macrophages. (1996)

It has long been known from the results of ultrastructural studies that complement- and immunoglobulin G (IgG)-opsonized particles are phagocytosed differently by macrophages (Kaplan. G. 1977. Scand. J. Immunol. 6:797-807). Complement-opsonized particles sink into the cell, whereas IgG-coated particles are engulfed by lamellipodia, which project from the cell surface. The molecular basis for these differences is unknown. We used indirect immunofluorescence and confocal microscopy to examine how cytoskeletal proteins associate with phagosomes containing complement-opsonized zymosan (COZ) particles or IgG beads in phorbol-myristateacetate-treated peritoneal macrophages. During ingestion of COZ, punctate structures rich in F-actin, vinculin, alpha-actinin, paxillin, and phosphotyrosine-containing proteins are distributed over the phagosome surface. These foci are detected beneath bound COZ within 30 s of warming the cells to 37 degrees C, and their formation requires active protein kinase C. By contrast, during Fc receptor-mediated phagocytosis, all proteins examined were uniformly distributed on or near the phagosome surface. Moreover, ingestion of IgG beads was blocked by tyrosine kinase inhibitors, whereas phagocytosis of COZ was not. Thus, the signals required for particle ingestion, and the arrangement of cytoskeletal proteins on the phagosome surface, vary depending upon which phagocytic receptor is engaged. Moreover, complement receptor (CR)-mediated internalization required intact microtubules and was accompanied by the accumulation of vesicles beneath the forming phagosome, suggesting that membrane trafficking plays a key role in CR-mediated phagocytosis.
Massachusetts Institute of Technology Reactor LEU Fuel Element Flow Test Conceptual Design – Hydraulic Reactor Design Parameters (2021)

The Massachusetts Institute of Technology Reactor (MITR-II, also referred to as MITR) is one of six U.S. high performance research reactors (USHPRR), including one critical facility, that is actively collaborating with the U.S. National Nuclear Security Administration (NNSA) Material Management and Minimization (M3) Reactor Conversion Program to convert to the use of low-enriched uranium (LEU, < 20 wt% 235U) fuel. The MIT Nuclear Reactor Laboratory has been working with the USHPRR Reactor Conversion (RC) Pillar at Argonne National Laboratory to perform fuel element design and fuel cycle performance analyses, steady-state thermal hydraulics safety analyses, and accident safety analyses in preparation for the conversion of MITR and support a preliminary Safety Analysis Report (SAR) for conversion to LEU fuel.
Efficient resource management in future heterogeneous wireless networks: The RIWCoS approach (2010)

Convergence of different wireless access networks and development towards 4G yields a necessity for more efficient resource management procedures in the integrated heterogeneous platform. The notion of resource is fundamentally different in heterogeneous environments (compared to homogeneous ones) as it is spanned across several different wireless solutions. This paper summarizes the development process of a novel architecture for resource management in heterogeneous wireless networks named RIWCoS. The architectural focal point is on the emerging IEEE 802.21 standard because of its ability to enable seamless and transparent vertical handovers. The paper shows simulation results of the IEEE 802.21 benefits and reports on a novel simulation analysis (using newly introduced metrics) of the RIWCoS architecture showing that it outperforms traditional serving policies of users in heterogeneous wireless environments. Moreover, the paper elaborates on a distinguished RIWCoS feature (the SAES algorithm) that enables efficient handling of emergency situations making the RIWCoS architecture suitable for military communications.
Magnetowetting and Sliding Motion of a Sessile Ferrofluid Droplet in the Presence of a Permanent Magnet (2010)

Motion of a droplet on a planar surface has applications in droplet-based lab on a chip technology. This paper reports the experimental results of the shape, contact angles, and motion of ferrofluid droplets driven by a permanent magnet on a planar homogeneous surface. The water-based ferrofluid in use is a colloidal suspension of single-domain magnetic nanoparticles. The effect of the magnetic field on the apparent contact angle of the ferrofluid droplet was first investigated. The results show that an increasing magnetic flux decreases the apparent contact angle of a sessile ferrofluid droplet. Next, the dynamic contact angle was investigated by observing the shape and the motion of a sessile ferrofluid droplet. The advancing and receding contact angles of the moving ferrofluid were measured at different moving speeds and magnetic field strengths. The measured contact angles were used to estimate the magnitude of the forces involved in the sliding motion. Scaling analysis was carried out to derive the critical velocity, beyond which the droplet is not able to catch up with the moving magnet.
Crystallization of diaminopimelate decarboxylase from<i>Escherichia coli</i>, a stereospecific<scp>D</scp>-amino-acid decarboxylase (2002)

The final step in lysine biosynthesis in bacteria, the conversion of meso-diaminopimelate to L-lysine, is catalyzed by the only known D-amino-acid decarboxylase, diaminopimelate decarboxylase (DDC). The Escherichia coli DDC has been cloned, overexpressed in E. coli with a carboxy-terminal polyhistidine purification tag and crystallized from lithium sulfate. The protein is intensely yellow, owing to the pyridoxal-5'-phosphate cofactor, and is enzymatically active. Large well ordered crystals, belonging to space group P6(1)22 with unit-cell parameters a = b = 98.6, c = 177 A, make high-resolution X-ray diffraction studies possible to characterize the residues important in stereospecific decarboxylation and reprotonation during catalytic turnover.
Role of hypoxia in cancer therapy by regulating the tumor microenvironment (2019)

Hypoxia targeting might be relevant to overcome hypoxia-associated resistance in cancer treatment.
Thermodilution cardiac output determination with a single flow-directed catheter (1973)

FORRESTER, JAMES S. MD; GANZA, WILLIAM MD; DIAMOND, GEORGE MD; HUGH, THOMAS MC MD; CHONETTE, DAVID W. MS; SWAN, H. J. C. MD, PH D Author Information