gebhart/patrepeval huggingface dataset:
Characterization of DNA Binding Protein of Porcine Adenovirus Type 3 (2001)

To identify and characterize the protein encoded by the E2A region of porcine adenovirus (PAV)-3, DNA sequence coding for a portion (amino acids 102–457) of the DNA binding protein (DBP) open reaching frame was cloned and expressed in <i>Escherichia coli</i> as a fusion protein with glutathione S-transferase protein of <i>Schistosoma japonica</i>. The affinity-purified fusion protein was used to immunize rabbits. Immunoprecipitation/Western blot analysis demonstrated that the antisera specifically recognized a protein of 50 kD in PAV-3-infected cells. Immunoperoxidase staining detected the DBP protein predominantly in the nucleus of the cells. Western blot analysis demonstrated that DBP was detected as early as 6 h after infection and remained detectable throughout the infection. Based on these results, a novel assay for quantitation of PAV-3 was established. The assay is less time consuming and can be performed in different porcine cells. In addition, virus titers determined by this assay are comparable to the standard plaque assay.
Cryogenic variable temperature ultrahigh vacuum scanning tunneling microscope for single molecule studies on silicon surfaces (2004)

The design and performance of a variable temperature ultrahigh vacuum (UHV) scanning tunneling microscope (STM) is presented. This STM operates from 8.2 to 300 K in a UHV environment with a base pressure of less than 6×10−11 Torr. Cooling is achieved from 300 to 80 K within 3.5 h and from 80 to 8.2 K within 4.5 h. The base temperature of 8.2 K is maintained at a liquid helium consumption rate of 0.9 l/h. This design allows for direct optical access to the tip-sample interface and direct line-of-sight dosing while the sample is mounted in the STM. The STM tip may be coarse translated laterally in two dimensions through a 6-mm-diam area at all temperatures. With the feedback loop off, the drift in the tip-sample spacing is approximately 0.008 Å/min at 8.2 K. Atomic resolution feedback controlled lithography is performed on hydrogen passivated Si(100) and differential tunneling conductance maps are gathered for isolated cyclopentene molecules on unpassivated Si(100), thus demonstrating that this system is well suited for studying single molecules on silicon surfaces from 8.2 to 300 K.
Solvent Extraction of Copper from a Silver Electrolyte (1987)

Abstract This paper presents a process for treatment of an AgNO3 electrolyte. Ag/Cu separation is done with conventional solvent extraction (SX). Cathode copper is electroworf in a diaphragm electrolysis cell while silver scrap is simultaneously dissolved anodically. The process proves to be a very effective recycling technique and is now being investigated on a pilot scale.
Recent achievements in anionic polymerization of (meth)acrylates (1999)

The constant progress of the anionic polymerization of (meth)acrylates is discussed from both the fundamental and practical points of view. A special attention is paid to the improved macromolecular engineering of (meth)acrylate-based (co)polymers. The resulting most important materials and the scaling-up process needed for their production are also emphasized. The recent developments witness for the healthy state of the anionic polymerization of these polar monomers. © 1999 John Wiley & Sons, Inc. J Polym Sci A: Polym Chem 37: 1–10, 1999
Hybrid electro-optic polymer/sol-gel waveguide directional coupler switches (2009)

We report electro-optic (EO) switches using transversely tapered hybrid EO/sol-gel waveguide directional couplers. The length of the active region critically reduces to 1.5 mm with transverse tapers, which is shorter by a factor of 5 than the active region with vertical tapers. The transitions are performed from a sol-gel core to two EO polymer cores with gradual index changes using gray-scaled photobleaching. The device shows the lowest switching voltage and an active length product (VsLa) of 2.9 V cm with a guest–host EO polymer AJLS102, compared to hybrid waveguide switches and modulators with vertical taper and higher EO coefficients.
Preparation of [3,3-(Ph3P)2-3-H-4-(polystyrylmethyl)-3,1,2-RhC2B9H10]. A polymer-bound metallocarborane catalyst (1977)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPreparation of [3,3-(Ph3P)2-3-H-4-(polystyrylmethyl)-3,1,2-RhC2B9H10]. A polymer-bound metallocarborane catalystB. A. Sosinsky, W. C. Kalb, R. A. Grey, V. A. Uski, and M. F. HawthorneCite this: J. Am. Chem. Soc. 1977, 99, 20, 6768–6771Publication Date (Print):September 1, 1977Publication History Published online1 May 2002Published inissue 1 September 1977https://pubs.acs.org/doi/10.1021/ja00462a056https://doi.org/10.1021/ja00462a056research-articleACS PublicationsRequest reuse permissionsArticle Views50Altmetric-Citations25LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Design Considerations for a New High Data Rate LWD Acoustic Telemetry System (2004)

Abstract The first commercial acoustic telemetry system was successfully introduced in 2000 as part of a drill stem testing system. However, environmentally challenging LWD offshore applications and a need for higher throughput required a complete redesign of that system. Thus, the new system incorporates a new high power acoustic transmitter driven by an advanced signal processor that maximizes input energy into particular frequencies and therefore minimizes dispersion and distortion effects. Low-frequency acoustic attenuators were specifically designed to attenuate in-band noise entering the communication system. In addition, two other features were introduced as a part of the complete communication system. They were (1) an in-line wireless link to pickup, digitize, and transmit signals off the pipe while rotating and (2) a high data rate acquisition and telemetry processing system. The system is designed to work solo or in conjunction with a mud pulse telemetry system. This paper will discuss the system design considerations and the data rates associated with different modes of operation as well as results of initial field tests.
Machine learning methods for in vivo skin parameter estimation (2013)

The WHO estimates three million new cases of skin cancer each year. Therefore, there exists a need for prescreening tools that can estimate the biological parameters of human skin, as they can help detect cancers before metastasis. In this paper, we present a novel inverse modeling technique based on Kubelka-Munk theory and machine learning to estimate biological skin parameters from in vivo hyperspec-tral imaging. We use the k-nearest neighbors (k-NN) algorithm in order to estimate skin parameters from their hy-perspectral signatures. We test our methods on 241 hyper-spectral signatures obtained from both genders and three ethnicities, and find encouraging results.
<title>Evaluation of variations of biomolecular constituents in human skin in vivo by near-infrared Raman spectroscopy</title> (2001)

A portable and rapid near-infrared (NIR) Raman spectroscopy system together with a tissue Raman probe was developed and utilized to acquire in vivo skin Raman spectra at 785 nm excitation. We demonstrated that good quality in vivo skin Raman spectra free of interferences of fiber fluorescence and silica Raman signals can be acquired within 5 seconds. Distinct Raman peaks in the 800-1800 cm<SUP>-1</SUP> range can be discerned clearly from various skin sites of the body. The intensity ratio of the Raman band at 1655 cm<SUP>-1</SUP> to that at 1445 cm<SUP>-1</SUP> was used to evaluate the variation of the protein/lipid depositions in the skin. The results show that the regional variations in the molecular composition and structure can be determined in vivo, suggesting that NIR Raman spectroscopy will be useful to non-invasively measure important biochemical parameters of human skin.
Evaluation of 24-Second Cyanide-Containing and Cyanide-Free Methods for Whole Blood Hemoglobin on the Technicon H*1™ Analyzer with Normal and Abnormal Blood Samples (1989)

Automated methods, with and without cyanide (+CN and -CN), for whole blood hemoglobin (Hb) determination were evaluated on the Technicon H*1TM System. Both automated Hb methods were linear over the range 0-250 g/L (0-25 g/dL) and correlated well with the International Committee for Standardization of Hematology (ICSH) reference method and with the Coulter S+II. Both methods quantitatively converted whole blood containing up to 100% carboxyhemoglobin in less than 24 seconds to their respective end products. With respect to abnormal samples (sickle cell anemia, multiple myeloma, and hyperlipemia), both H*1 methods gave Hb results that were equivalent to the (postfiltration) ICSH method. For samples with white blood cell (WBC) counts less than 36 X 10(9)/L, the +CN method was equivalent to the (postfiltration) ICSH method, whereas for WBC counts greater than 20 X 10(9)/L, the -CN method showed acceptable recovery of the mean but unacceptable imprecision. For WBC counts of 36-164 X 10(9)/L, the +CN method yielded acceptable Hb recovery with unacceptable imprecision. Hyperlipemia, resulting from addition of Intralipid directly to the blood samples, caused large errors in both H*1 methods.
Isolation and characterization of enterotoxin-deficient mutants of Escherichia coli. (1978)

The genes controlling the production of two types of enterotoxin of Escherichia coli, one heat-labile (LT) and the other heat-stable (ST), are found on plasmids. The absence of a direct selection procedure has made it difficult to isolate mutants affecting toxin production. However, the availability of a naturally occurring "recombinant" plasmid, carrying genes for LT and ST formation and also for resistance to tetracycline, streptomycin, and sulfonamides, made it possible to use comutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine to enrich for such mutants. We have isolated and characterized 58 LT- mutants and 7 ST- mutants. Among the LT- group we found amber mutants, temperature-sensitive mutants (most of which produce unusually heat-labile LT), and "leaky" mutants with reduced LT activity. The majority of the tested LT- mutants produced immunologically crossreacting material, in most cases in wild-type amounts. Among all 17 of the LT- mutants that could be transferred, the mutation was found to be on the plasmid. In contrast, only one of four transferrable ST- mutants appeared to be a plasmid mutant.
Clinical results of ultrasound bladder vibrometry for assessment of bladder compliance (2016)

Healthy human bladder is a viscoelastic shell capable of expanding to allow storage of urine at low pressures. The ability to maintain low pressures as the urine volume increases is called bladder compliance. Compliance is a key in proper functioning of urinary system. Spinal cord injuries and other neurogenic diseases can cause deterioration in bladder expansion capability (e.g., through excessive growth of fibrotic tissue), which in turn can increase the rigidity of the wall and reduce bladder compliance. Urodynamic study (UDS) is the clinical standard for evaluation of the bladder compliance through observation of bladder pressure-volume behavior. However, this method is costly and invasive. Ultrasound bladder vibrometry (UBV) has been introduced as noninvasive technique for evaluation of bladder viscoelasticity through excitation of the bladder using ultrasound radiation force and tracking the resulting Lamb waves. Here, we present the results of UBV from 44 patients undergoing concurrent UDS and UBV studies. Our results show that elasticity parameters obtained from the UBV closely correlate with the UDS pressure-volume data with a median Pearson’s correlation value of more than 0.8. These results prove the potential utility of UBV as an alternative noninvasive method for bladder compliance assessment. [Work supported by NIH grant DK99231.]
Abstract 19759: Improvement of Diastolic Function by Exosome Injection in a Rat Model of Heart Failure With Preserved Ejection Fraction (2016)

Introduction: Cardiosphere-derived cells (CDCs) improve diastolic function when injected in rats with heart failure with preserved ejection fraction (HFpEF) by decreasing inflammation and fibrosis....
Enhancement of metal oxide deposition rate and quality using pulsed plasma-enhanced chemical vapor deposition at low frequency (2008)

The deposition rate and quality of alumina thin films fabricated by plasma-enhanced chemical vapor deposition (PECVD) increased significantly when square wave power modulation was applied at low frequency (∼1Hz). The pulsed PECVD rate was enhanced by a factor of ∼3 relative to continuous wave operation, and the quantity of impurities was dramatically attenuated. Deposition experiments on trenches with aspect ratios ranging from 4 to infinity demonstrated that the technique achieves a high degree of conformality. Important reactor design and operating considerations are described. Pulsed PECVD produced similar quality improvements for Ta2O5, TiO2, and ZnO, suggesting that the approach has widespread potential for metal oxide synthesis.
Fiber design for high-power fiber lasers (2009)

This paper reviews the progress in active fibers suitable for power scaling, highlighting the advances in fiber design that will enable the control of nonlinearities such as SRS and SBS in high power fiber lasers, as well as making feasible a practical high power three-level system.
1.0–1.6 μm planar avalanche photodiode by LPE grown InP/InGaAs/InP DH structure (1982)

A planar avalanche photodiode is for the first time fabricated using the (111)A face of InP/InGaAs/InP double-hetero-(DH)-structure. The top InP layer where the carrier-multiplication takes place is directly grown on the In0.53Ga0.47As layer by liquid phase epitaxy. Maximum avalanche multiplication factors of 25 are obtained in a wavelength range of 1.0–1.6 μm. Quantum efficiencies are 70–80% at this wavelength range.
Speaker identification improvement using the usable speech concept (2004)

Most signal processing involves processing a signal with-out concern for the quality or information content of that signal. In speech processing, speech is processed on a frame-by-frame basis, usually only with concern that the frame is either speech or silence. However, knowing how reli-able the information is in a frame of speech can be very important and useful. This is where usable speech detec-tion and extraction can play a very important role. The us-able speech frames can be defined as frames of speech that contain higher information content compared to unusable frames with reference to a particular application. We have been investigating a speaker identification system to iden-tify usable speech frames and then to determine a method for identifying those frames as usable using a different ap-proach. A 100 % accuracy can be achieved in speaker identi-fication by using only the extracted usable speech segments. 1.
ChemInform Abstract: A Convenient Route to Tetraalkylammonium Perfluoroalkoxides from Hydrofluoroethers. (2015)

Abstract Perfluoroalkyl methyl ether acts as methylating reagent on tertiary aliphatic amines.
Regional Delivery of Chimeric Antigen Receptor–Engineered T Cells Effectively Targets HER2+ Breast Cancer Metastasis to the Brain (2017)

<b>Purpose:</b> Metastasis to the brain from breast cancer remains a significant clinical challenge, and may be targeted with CAR-based immunotherapy. CAR design optimization for solid tumors is crucial due to the absence of truly restricted antigen expression and potential safety concerns with "on-target off-tumor" activity. Here, we have optimized HER2-CAR T cells for the treatment of breast to brain metastases, and determined optimal second-generation CAR design and route of administration for xenograft mouse models of breast metastatic brain tumors, including multifocal and leptomeningeal disease.<b>Experimental Design:</b> HER2-CAR constructs containing either CD28 or 4-1BB intracellular costimulatory signaling domains were compared for functional activity <i>in vitro</i> by measuring cytokine production, T-cell proliferation, and tumor killing capacity. We also evaluated HER2-CAR T cells delivered by intravenous, local intratumoral, or regional intraventricular routes of administration using <i>in vivo</i> human xenograft models of breast cancer that have metastasized to the brain.<b>Results:</b> Here, we have shown that HER2-CARs containing the 4-1BB costimulatory domain confer improved tumor targeting with reduced T-cell exhaustion phenotype and enhanced proliferative capacity compared with HER2-CARs containing the CD28 costimulatory domain. Local intracranial delivery of HER2-CARs showed potent <i>in vivo</i> antitumor activity in orthotopic xenograft models. Importantly, we demonstrated robust antitumor efficacy following regional intraventricular delivery of HER2-CAR T cells for the treatment of multifocal brain metastases and leptomeningeal disease.<b>Conclusions:</b> Our study shows the importance of CAR design in defining an optimized CAR T cell, and highlights intraventricular delivery of HER2-CAR T cells for treating multifocal brain metastases. <i>Clin Cancer Res; 24(1); 95-105. ©2017 AACR</i>.
Hearing aid with programmable remote and method of deriving settings for configuring the hearing aid (1994)

Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter Facebook Reddit LinkedIn Tools Icon Tools Reprints and Permissions Cite Icon Cite Search Site Citation Bruce A. Brillhart, Theodore P. Adams; Hearing aid with programmable remote and method of deriving settings for configuring the hearing aid. J. Acoust. Soc. Am. 1 October 1994; 96 (4): 2620. https://doi.org/10.1121/1.411313 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAcoustical Society of AmericaThe Journal of the Acoustical Society of America Search Advanced Search |Citation Search
Holey fibres - a review of recent developments in theory, fabrication and experiment (2000)

Recent advances in the development of holey optical fibres are described. We review the basic physical properties of the various fibre types, the status of current fibre fabrication efforts and discuss numerous end applications.
Sphingosine 1-Phosphate Modulates Spinal Nociceptive Processing (2008)

Sphingosine 1-Phosphate (S1P) modulates various cellular functions such as apoptosis, cell differentiation, and migration. Although S1P is an abundant signaling molecule in the central nervous system, very little is known about its influence on neuronal functions. We found that S1P concentrations were selectively decreased in the cerebrospinal fluid of adult rats in an acute and an inflammatory pain model. Pharmacological inhibition of sphingosine kinases (SPHK) decreased basal pain thresholds and SphK2 knock-out mice, but not SphK1 knock-out mice, had a significant decrease in withdrawal latency. Intrathecal application of S1P or sphinganine 1-phosphate (dihydro-S1P) reduced the pain-related (nociceptive) behavior in the formalin assay. S1P and dihydro-S1P inhibited cyclic AMP (cAMP) synthesis, a key second messenger of spinal nociceptive processing, in spinal cord neurons. By combining fluorescence resonance energy transfer (FRET)-based cAMP measurements with Multi Epitope Ligand Cartography (MELC), we showed that S1P decreased cAMP synthesis in excitatory dorsal horn neurons. Accordingly, intrathecal application of dihydro-S1P abolished the cAMP-dependent phosphorylation of NMDA receptors in the outer laminae of the spinal cord. Taken together, the data show that S1P modulates spinal nociceptive processing through inhibition of neuronal cAMP synthesis.
A molecular Fe-complex as a catalyst probe for in-gel visual detection of proteins <i>via</i> signal amplification (2015)

We report the use of a molecular peroxidase mimic biuret-Fe-TAML for chemoselective labeling of proteins and the subsequent visual detection (<0.1 pmoles) of the conjugate in a polyacrylamide gel by catalytic signal amplification. Use of this probe in activity based protein profiling (ABPP) of serine proteases is also demonstrated.
Cover Picture: Colloidal Synthesis of Hollow Cobalt Sulfide Nanocrystals (Adv. Funct. Mater. 11/2006) (2006)

Abstract Hollow nanocrystals have been synthesized through a mechanism analogous to the Kirkendall Effect. When a cobalt nanocrystal reacts with sulfur in solution, the outward diffusion of cobalt atoms is faster than the inward diffusion of sulfur atoms through the sulfide shell. The dominating outward diffusion of cobalt cations produces vacancies that can condense into a single void in the center of the nanocrystal at high temperatures. This process provides a general route to the synthesis of hollow nanostructures of a large number of compounds and is described in the Full Paper by A. P. Alivisatos and co‐workers on p. 1389. Formation of cobalt sulfide hollow nanocrystals through a mechanism similar to the Kirkendall Effect has been investigated in detail. It is found that performing the reaction at > 120 °C leads to fast formation of a single void inside each shell, whereas at room temperature multiple voids are formed within each shell, which can be attributed to strongly temperature‐dependent diffusivities for vacancies. The void formation process is dominated by outward diffusion of cobalt cations; still, the occurrence of significant inward transport of sulfur anions can be inferred as the final voids are smaller in diameter than the original cobalt nanocrystals. Comparison of volume distributions for initial and final nanostructures indicates excess apparent volume in shells, implying significant porosity and/or a defective structure. Indirect evidence for fracture of shells during growth at lower temperatures was observed in shell‐size statistics and transmission electron microscopy images of as‐grown shells. An idealized model of the diffusional process imposes two minimal requirements on material parameters for shell growth to be obtainable within a specific synthetic system.