gebhart/patrepeval huggingface dataset:
2′-Benzoyloxycinnamaldehyde Induces Apoptosis in Human Carcinoma via Reactive Oxygen Species (2004)

2'-hydroxycinnamaldehyde (HCA) has been shown to have inhibitory effects on farnesyl protein transferase in vitro, angiogenesis, and tumor cell growth. However, mechanism for these inhibitions remains unknown. As a derivative of HCA, BCA (2'-benzoyl-oxycinnamaldehyde) was synthesized by replacing hydroxyl group with benzoyl-oxyl group. When p53-mutated cancer cell lines (MDA-MB-231 breast cancer cell and SW620 colon cancer cell) were treated with 10 microM HCA or BCA, it induced growth arrest and apoptosis of tumor cells. Markers of apoptosis such as degradations of chromosomal DNA and poly(ADP-ribose) polymerase and activation of caspase-3 were detected after HCA or BCA treatment. BCA-induced apoptosis was blocked by pretreatment of cells with anti-oxidants, glutathione, or N-acetyl-cysteine. In addition, BCA-induced activation of caspase-3 and degradation of poly(ADP-ribose) polymerase were abolished by pretreatment of cells with the anti-oxidants. These results suggest that reactive oxygen species are major regulator of BCA-induced apoptosis. HCA or BCA-induced accumulation of reactive oxygen species was detected by using DCF-DA, an intracellular probe of oxidative stress. Furthermore, when BCA (100 mg/kg) was administrated intraperitoneally or orally into a nude mouse, it inhibited >88 or 41% of tumor growth, respectively, without any detectable weight change. These results suggest that BCA is a good drug candidate for cancer therapy.
Affinity Separations (2001)

This chapter contains sections titled: Introduction Affinity Adsorption Chromatography Other Affinity Techniques New or Improved Concepts and Applications Summary References
Instrumentation for low noise nanopore-based ionic current recording under laser illumination (2018)

We describe a nanopore-based optofluidic instrument capable of performing low-noise ionic current recordings of individual biomolecules under laser illumination. In such systems, simultaneous optical measurements generally introduce significant parasitic noise in the electrical signal, which can severely reduce the instrument sensitivity, critically hindering the monitoring of single-molecule events in the ionic current traces. Here, we present design rules and describe simple adjustments to the experimental setup to mitigate the different noise sources encountered when integrating optical components to an electrical nanopore system. In particular, we address the contributions to the electrical noise spectra from illuminating the nanopore during ionic current recording and mitigate those effects through control of the illumination source and the use of a PDMS layer on the SiN<sub>x</sub> membrane. We demonstrate the effectiveness of our noise minimization strategies by showing the detection of DNA translocation events during membrane illumination with a signal-to-noise ratio of ∼10 at 10 kHz bandwidth. The instrumental guidelines for noise minimization that we report are applicable to a wide range of nanopore-based optofluidic systems and offer the possibility of enhancing the quality of synchronous optical and electrical signals obtained during single-molecule nanopore-based analysis.
Characterization of the Outer Membrane Proteome of <i>Leptospira interrogans</i> Expressed during Acute Lethal Infection (2006)

Pathogenic Leptospira species adapt to a wide range of environmental conditions during disease transmission and infection. While the proteome of in vitro cultivated Leptospira has been characterized in several studies to date, relatively little is known of the proteome as expressed by Leptospira during disease processes. Isolates of Leptospira obtained from patients suffering the severe pulmonary form of leptospirosis cause acute lethal infection in guinea pigs and chronic asymptomatic infection in rats. Recent studies have demonstrated that protein and lipopolysaccharide constituents of Leptospira recovered from acutely infected guinea pig tissue differ from that of Leptospira in chronically infected rat tissue and in vitro cultivated Leptospira (J. E. Nally, E. Chow, M. C. Fishbein, D. R. Blanco, and M. A. Lovett, Infect. Immun. 73:3251-3260, 2005). In the current study, the proteome of Leptospira expressed during disease processes was characterized relative to that of in vitro cultivated Leptospira (IVCL) after enrichment for hydrophobic membrane proteins with Triton X-114. Protein samples were separated by two-dimensional gel electrophoresis, and antigens expressed during infection were identified by immunoblotting with monospecific antiserum and convalescent rat serum in addition to mass spectrometry. Results suggest a significant increase in the expression of the outer membrane protein Loa22 during acute infection of guinea pigs relative to other outer membrane proteins, whose expression is generally diminished relative to expression in IVCL. Significant amounts of LipL32 are also expressed by Leptospira during acute infection of guinea pigs.
A Study on Online-Shopping Opinion Leaders Based on Massive Data (2018)

The online communities are important channels for consumers to distribute, transmit and access word-of-mouth information. However, the influence power of the users has a significant difference. This article selects about 200 Online Communities (taobao wangwang group), based on more than one million taobao real transaction data provided by nearly fifty thousand members of the communities, from the perspective of social network to build weighted and directed graph of online shopping interpersonal influence relations and usersâ influences model, mining online shopping opinion leaders and exploring the community online communication, spread and effect regularities to provide theoretical basis and practice guidance for word-of-mouth marketing.
Effects of circuit nonlinearities on dynamic dead time optimization for a three-phase microinverter (2017)

This paper investigates the effect of circuit component nonlinearities on dead time optimization for a three-phase microinverter with a peak current mode zero voltage switching (ZVS) output stage. The primary nonlinearities are those associated with MOSFET and other switching devices parasitic capacitance which may vary significantly as a function of the device operating voltage. In addition, filter inductor values vary as a function of operating current. The effect of the dynamic nature of these circuit components on inverter efficiency and dead time optimization is analyzed. Both calculated and experimental results are obtained using a 400-W three-phase half-bridge microinverter prototype.
Hydrolysis of GTP by p21NRAS, the NRAS protooncogene product, is accompanied by a conformational change in the wild-type protein: use of a single fluorescent probe at the catalytic site. (1990)

2'(3')-O-(N-Methyl)anthraniloylguanosine 5'-triphosphate (mantGTP) is a fluorescent analogue of GTP that has similar properties to the physiological substrate in terms of its binding constant and the kinetics of its interactions with p21NRAS, the NRAS protooncogene product. There is a 3-fold increase in fluorescence intensity when mantGTP binds to p21NRAS. The rate constant for the cleavage of mantGTP complexed with the protein is similar to that of GTP, and cleavage is accompanied by a fluorescence intensity change in the wild-type protein complex. A two-phase fluorescence change also occurs when the nonhydrolyzable analogue 2'(3')-O-(N-methyl)anthraniloylguanosine 5'-[beta, gamma-imido]triphosphate (mantp[NH]ppG) binds to wild-type p21NRAS. The second phase occurs at the same rate as the second phase observed after mantGTP binding. Thus this second phase is probably a conformation change of the p21NRAS nucleotiside triphosphate complex and that the change controls the rate of GTP hydrolysis on the protein. With a transforming mutant, [Asp12]-p21NRAS, there is no second phase of the fluorescence change after mantGTP or mantp[NH]ppG binding, even though mantGTP is hydrolyzed. This shows that an equivalent conformational change does not occur and thus the mutant may stay in a "GTP-like" conformation throughout the GTPase cycle. These results are discussed in terms of the proposed role of p21NRAS in signal transduction and the transforming properties of the mutant.
An Introduction to Practical Formal Methods Using Temporal Logic (2011)

The name "temporal logic" may sound complex and daunting; but while they describe potentially complex scenarios, temporal logics are often based on a few simple, and fundamental, concepts - highlighted in this book. An Introduction to Practical Formal Methods Using Temporal Logic provides an introduction to formal methods based on temporal logic, for developing and testing complex computational systems. These methods are supported by many well-developed tools, techniques and results that can be applied to a wide range of systems.<br/><br/>Fisher begins with a full introduction to the subject, covering the basics of temporal logic and using a variety of examples, exercises and pointers to more advanced work to help clarify and illustrate the topics discussed. He goes on to describe how this logic can be used to specify a variety of computational systems, looking at issues of linking specifications, concurrency, communication and composition ability. He then analyses temporal specification techniques such as deductive verification, algorithmic verification, and direct execution to develop and verify computational systems. The final chapter on case studies analyses the potential problems that can occur in a range of engineering applications in the areas of robotics, railway signalling, hardware design, ubiquitous computing, intelligent agents, and information security, and explains how temporal logic can improve their accuracy and reliability.<br/><br/>- Models temporal notions and uses them to analyze computational systems<br/>Provides a broad approach to temporal logic across many formal methods - including specification, verification and implementation<br/>- Introduces and explains freely available tools based on temporal logics and shows how these can be applied<br/>- Presents exercises and pointers to further study in each chapter, as well as an accompanying website providing links to additional systems based upon temporal logic as well as additional material related to the book.
Sotos syndrome associated with a de novo balanced reciprocal translocation t(5;8)(q35;q24.1) (2001)

We describe a de novo balanced reciprocal translocation between the long arms of chromosomes 5 and 8 [46,XX,t(5;8)(q35;q24.1)] in a 15-month-old girl with a typical Sotos syndrome phenotype. Involvement of the 5q35 region was previously reported (Maroun et al. [1994: Am J Med Genet 50:291-293]) as one of translocation breakpoints in the present patient. We suggest that the gene responsible for Sotos syndrome is located to a distal long-arm region of chromosome 5.
Secure connections in Bluetooth scatternets (2003)

Bluetooth is a wireless networking technology specifically developed for personal area networking and other short range applications. Bluetooth devices can be networked together by forming piconets of up to eight devices, where a single device is the master and the rest are slaves. These piconets can further be interconnected to create a scatternet. A scatternet is formed by allowing bridge nodes to operate in multiple piconets by interleaving their membership. These bridge nodes alternate between piconets by scheduling their participation during disjoint time-slots. Scatternet formation and routing are important problems for the future of Bluetooth. Another equally important issue is security. We describe how Bluetooth security is currently implemented and discuss a number of security attacks. We then describe two scatternet scenarios that warrant secure operation, and propose solutions that meet our security goals of confidentiality, availability, and integrity. A secure scatternet is created by generating contributory link keys between interconnected piconets. We also introduce a new concept that we call Private PANs. A Private PAN is a piconet in which devices are bonded together, and generally also controlled, and. owned, by the same entity. The main purpose of this paper is to extend the scope of Bluetooth security to scatternets.
Thermal sensor allocation and full-system temperature characterization for thermal-aware mesh-based NoC system by using compressive sensing technique (2017)

As the complexity of multicore system grows with respect to the technology development, the large power density of multicore systems makes the thermal problems become severer. To prevent the multicore systems from overheating, in the practical way, some thermal sensors, sensing temperature at few selected locations, are embedded in the system. Then, the sensing temperatures are used to perform dynamic thermal managements (DTMs). However, searching the appropriate locations for those number limited thermal sensors is an NP-hard problem, which is time-consuming to find the optimal sensor allocations. In this paper, we demonstrate that the on-chip thermal gradients lead to sparse signals in the frequency domain. Based on this sparse-signal characteristic, we first apply the compressive sensing theory to find the thermal sensor allocations. Compressive sensing technique aims to recover the original signal from the fewer samples than the required by the Nyquist theorem. By using low-complexity random thermal sensor allocation method and Stagewise Orthogonal Matching Pursuit (StOMP)-based full-system temperature characterization, we can reduce 49% average error of full-system temperature characterization. Besides, under a constraint of full-system temperature characterization error, the proposed method can reduce 53% average number of adopted thermal sensors.
Hepatic portoenterostomy (the Kasai operation) for biliary atresia. (1975)

Twenty-two infants and children with extrahepatic biliary atresia were treated by Kasai's hepatic portoenterostomy operation since 1972. Postoperative bile drainage occurred in 11 infants. Success never was obtained in patients older than 12 weeks nor in the absence of microscopic biliary ductules in the excised fibrotic remnant of the extrahepatic bile ducts. With these exclusions almost 90 percent of infants with biliary atresia had sustained bile drainage after operation. In the early cases, ascending cholangitis was a major and recurrent complication with sequellae in many instances of permanent jaundice and probably irreversible liver damage. A new and simple modification of Kasai's original operation has prevented this complication thus far. Despite apparently normal bile drainage, postoperative studies by transhepatic cholangiography in some of these patients have demonstrated a grossly abnormal hepatobiliary intrastructure, suggesting that an underlying liver involvement may be present in biliary atresia.
Effect of Photocrosslinkable Chitosan Hydrogel and Its Sponges to Stop Bleeding in a Rat Liver Injury Model (2010)

This study examined the hemostatic efficacy of photocrosslinkable chitosan hydrogel-mixed photocrosslinked chitosan sponges (PCM-S) after hepatic injury in rats. The left lobe of the liver was penetrated with a dermal punch to produce a penetrating wound in heparinized and nonheparinized rats. Treated rats either had PCM-S applied into the wound and then were immediately ultraviolet irradiated, or they had TachoComb (TC) inserted into the wound. Blood loss, hemostasis, and survival were quantified after the hepatic injury. Measurements on serum alanine aminotransferase in nonheparinized rats and hemoglobin concentrations and histologic examinations in heparinized rats were performed to assess hepatic function. Although the hemostatic effect in the PCM-S-treated nonheparinized rats was identical to that of the TC-treated group, PCM-S-treatment has higher hemostatic effect in heparinized rats. No adverse events related to the use of PCM-S were detected in blood and histologic examinations.
Reversible Electrochemical Insertion of Lithium in Fine Grained Polycrystalline Powders of SnO<sub>2</sub> (1994)

Fine grained SnO 2 powders have been obtained using an unconventional method. It deals with the well known polymerization method starting from the metallic halide SnCl 4 with polyethylene oxide (PEO). With this method, SnO 2 powders, which are free from water and hydroxyl group contaminations and possess small crystallite size (), are obtained by appropriate pyrolysis of the polymer. Consequently, these powders show good ability to insert reversibly lithium ions in the Li/Li + /Li x SnO 2 cell. Indeed, by minimizing the size of the crystallites, the formation of defect‐bonds is favored, particularly at the crystallite surface, acting as reversible (de)grafting sites of Li + . Finally, an easy‐to‐carry out method to determine the chemical diffusion coefficient of lithium has been proposed.
Addictions (2017)

In 2016, the actuality for addictions in this edition addresses four points. Social neurosciences of addiction are of great importance regarding the vulnerabilities for addiction and for the recovery. Deep brain stimulation is emerging in the therapeutic panel coming from the clinical neurosciences for the addictions. Novelties in opioid agonists for the treatment of opiates dependence, with the apparition in the Swiss market of release morphine and of levomethadone. Cannabis and prison, a pilot study for the maintenance of abstinence in prison.
Golf Swing Motion Tracking Using Inertial Sensors and a Stereo Camera (2013)

A golf swing motion tracking algorithm is proposed in which the golf club trajectory (position and velocity) and club face orientation information are given. Two sensors are used in the algorithm: an inertial sensor unit on the golf club, and a stereo camera that captures infrared light emitting diodes (LEDs), also on the golf club. During the address and impact golf swing phases, the camera captures the infrared LEDs on the golf club. Using the infrared LEDs as landmarks, the position and orientation of the golf club are computed. When the golf club is moving, an inertial navigation algorithm is used to compute the golf club trajectory. An indirect Kalman filter with nine states is used to combine the inertial sensor and vision data. The average position accuracy is about 3.6 cm and the maximum error is about 13.2 cm. The proposed system can be used to analyze golf swings quantitatively.
Operating Channel Validation (2018)

We present a backwards compatible extension to the 802.11 standard to prevent multi-channel man-in-the-middle attacks. This extension authenticates parameters that define the currently in-use channel.
Site-specific protein immobilization through N-terminal oxime linkages (2007)

Immobilizing proteins in specific orientations is important for diagnostic protein arrays, biomaterials, and other applications where retention of bioactivity is essential. We report an approach for protein micropatterning that exploits a chemoselective reaction to conjugate proteins at the N-terminus to polymer films. A copolymer from 2-hydroxyethyl methacrylate and a Boc-protected aminooxy tetra(ethylene glycol) methacrylate was synthesized by radical polymerization. Boc groups were locally deprotected using photoacid generator-based photolithography. Micropatterns were verified by fluorescence microscopy utilizing green fluorescent aldehyde microspheres. Streptavidin that was subjected to a transamination reaction to install an α-ketoamide group at the N-terminus was conjugated to the patterns by oxime bond formation. Since the majority of proteins may be modified to contain a reactive carbonyl group, this methodology should be applicable to pattern a wide variety of proteins specifically through the N-terminus.
Role of the kidney in the metabolism of apolipoprotein A-IV: influence of the type of proteinuria (2006)

Increased plasma concentrations of apolipoprotein A-IV (apoA-IV) in chronic renal disease suggest a metabolic role of the kidney for this antiatherogenic protein. Therefore, we investigated patients with various forms of proteinuria and found increased serum concentrations of apoA-IV in 124 nephrotic patients compared with 274 controls (mean 21.9 +/- 9.6 vs. 14.4 +/- 4.0 mg/dl; P < 0.001). Decreasing creatinine clearance showed a strong association with increasing apoA-IV levels. However, serum albumin levels significantly modulated apoA-IV levels in patients with low creatinine clearance, resulting in lower levels of apoA-IV in patients with low compared with high albumin levels (21.4 +/- 8.6 vs. 29.2 +/- 8.4 mg/dl; P = 0.0007). Furthermore, we investigated urinary apoA-IV levels in an additional 66 patients with a wide variety of proteinuria and 30 controls. Especially patients with a tubular type of proteinuria had significantly higher amounts of apoA-IV in urine than those with a pure glomerular type of proteinuria and controls (median 45, 14, and 0.6 ng/mg creatinine, respectively). We confirmed these results in affected members of a family with Dent's disease, who are characterized by an inherited protein reabsorption defect of the proximal tubular system. In summary, our data demonstrate that the increase of apoA-IV caused by renal impairment is significantly modulated by low levels of serum albumin as a measure for the severity of the nephrotic syndrome. From this investigation of apoA-IV in urine as well as earlier immunohistochemical studies, we conclude that apoA-IV is filtered through the normal glomerulus and is subsequently reabsorbed mainly by proximal tubular cells.
Leukocyte Aggregation Response to Quantitative Plasma Levels of C3a and C5a (1986)

Granulocyte aggregation (GA) response has previously been described as a sensitive assay for complement activation in sepsis. Complement component C5a has been implicated as the plasma factor responsible for GA. The quantitative interaction of complement components C3a and C5a with GA, however, is not clearly defined. This study evaluates the relationship of GA responses to plasma levels of C5a and C3a in zymosan-activated plasma (ZAP). The C3a and C5a levels were measured by radioimmunoassay in serial dilutions of ZAP. Granulocyte aggregation responses of normal human leukocytes were determined for each ZAP dilution. The C5a levels in a 1:16 dilution of ZAP were higher than in normal plasma (22 +/- 7 vs 9 +/- 3 ng/mL), as were GA responses (24 +/- 1 vs 11 +/- 2 percentage of maximum light transmission). The C3a levels in a 1:8 dilution of ZAP are elevated above those of normal plasma (656 +/- 167 vs 411 +/- 29 ng/mL). Correlation coefficients were .9809 for C3a vs GA, .9788 for C5a vs G, and .9860 for C3a vs C5a. Complement components C3a and C5a are involved in GA in vitro. Granulocyte aggregometry can detect low levels of activated complement in ZAP but may not be specific for C5a. The relative contribution of C3a and C5a to observed GA is not clear from the data.