gebhart/patrepeval huggingface dataset:
Effects of arginine on heat‐induced aggregation of concentrated protein solutions (2011)

Arginine is one of the commonly used additives to enhance refolding yield of proteins, to suppress aggregation of proteins, and to increase solubility of proteins, and yet the molecular interactions that contribute to the role of arginine are unclear. Here, we present experiments, using bovine serum albumin (BSA), lysozyme (LYZ), and β-lactoglobulin (BLG) as model proteins, to show that arginine can enhance heat-induced aggregation of concentrated protein solutions, contrary to the conventional belief that arginine is a universal suppressor of aggregation. Results show that the enhancement in aggregation is caused only for BSA and BLG, but not for LYZ, indicating that arginine's preferential interactions with certain residues over others could determine the effect of the additive on aggregation. We use this previously unrecognized behavior of arginine, in combination with density functional theory calculations, to identify the molecular-level interactions of arginine with various residues that determine arginine's role as an enhancer or suppressor of aggregation of proteins. The experimental and computational results suggest that the guanidinium group of arginine promotes aggregation through the hydrogen-bond-based bridging interactions with the acidic residues of a protein, whereas the binding of the guanidinium group to aromatic residues (aggregation-prone) contributes to the stability and solubilization of the proteins. The approach, we describe here, can be used to select suitable additives to stabilize a protein solution at high concentrations based on an analysis of the amino acid content of the protein.
Applications of supercritical CO<sub>2</sub> extraction to microalgae and plants (1995)

Abstract A flow apparatus was constructed to carry out studies of supercritical fluid extraction in the temperature range 298·15–353·15 K and pressures up to 40 MPa. To test the apparatus, studies on the solubility of naphthalene in CO 2 at 308·15, 313·15 and 318·15 K and pressures up to 35 MPa were carried out. These experimental data were correlated through the Peng–Robinson equation of state. Samples of the microalgae Botryococcus braunii and Chlorella vulgaris which produce diolefines C 25 –C 31 and carotenoids, respectively, were submitted to supercritical CO 2 . The extraction yields for these compounds were studied and compared with those obtained using organic solvents. Supercritical CO 2 was also used to produce extracts from rosemary leaves ( Rosmarinus officinalis L.), which were compared with those obtained by hexane extraction.
Mounted Smartphones as Measurement and Control Platforms for Motor-Based Laboratory Test-Beds (2016)

Laboratory education in science and engineering often entails the use of test-beds equipped with costly peripherals for sensing, acquisition, storage, processing, and control of physical behavior. However, costly peripherals are no longer necessary to obtain precise measurements and achieve stable feedback control of test-beds. With smartphones performing diverse sensing and processing tasks, this study examines the feasibility of mounting smartphones directly to test-beds to exploit their embedded hardware and software in the measurement and control of the test-beds. This approach is a first step towards replacing laboratory-grade peripherals with more compact and affordable smartphone-based platforms, whose interactive user interfaces can engender wider participation and engagement from learners. Demonstrative cases are presented in which the sensing, computation, control, and user interaction with three motor-based test-beds are handled by a mounted smartphone. Results of experiments and simulations are used to validate the feasibility of mounted smartphones as measurement and feedback control platforms for motor-based laboratory test-beds, report the measurement precision and closed-loop performance achieved with such platforms, and address challenges in the development of platforms to maintain system stability.
Synthesis of a novel lipopeptide with α‐melanocyte‐stimulating hormone peptide ligand and its effect on liposome stability (1999)

Introduction of liposomes into target cells is important for drug delivery systems. For this purpose, the surface of the liposome is equipped with ligand peptides, which may bind to specific receptors on the cell membrane. An artificial novel lipopeptide (MSH-C4A2) containing the alpha-melanocyte-stimulating hormone (alpha-MSH) sequence and two long alkyl chains was designed and synthesized, and the liposome, composed of egg phosphatidylcholine (EPC) and MSH-C4A2, was prepared. The stability of the liposome was estimated by measuring calcein leakage from the liposome inner phase. The stability of the liposome decreased upon addition of MSH-A4C2, which seemed to be attributable to the amphiphilic property of the peptide moiety (alpha-MSH) of MSH-A2C4. The stability was, however, recovered fairly well upon addition of cholesterol (Ch) or phosphatidylglycerol (PG). It was concluded therefore that the ternary system, MSH-C4A2/Ch/EPC or MSH-C4A2/PG/EPC, is suitable for preparing the functional liposome.
Mesenchymal Stem Cells and Their Subpopulation, Pluripotent Muse Cells, in Basic Research and Regenerative Medicine (2013)

Mesenchymal stem cells (MSCs) have gained a great deal of attention for regenerative medicine because they can be obtained from easy accessible mesenchymal tissues, such as bone marrow, adipose tissue, and the umbilical cord, and have trophic and immunosuppressive effects to protect tissues. The most outstanding property of MSCs is their potential for differentiation into cells of all three germ layers. MSCs belong to the mesodermal lineage, but they are known to cross boundaries from mesodermal to ectodermal and endodermal lineages, and differentiate into a variety of cell types both in vitro and in vivo. Such behavior is exceptional for tissue stem cells. As observed with hematopoietic and neural stem cells, tissue stem cells usually generate cells that belong to the tissue in which they reside, and do not show triploblastic differentiation. However, the scientific basis for the broad multipotent differentiation of MSCs still remains an enigma. This review summarizes the properties of MSCs from representative mesenchymal tissues, including bone marrow, adipose tissue, and the umbilical cord, to demonstrate their similarities and differences. Finally, we introduce a novel type of pluripotent stem cell, multilineage-differentiating stress-enduring (Muse) cells, a small subpopulation of MSCs, which can explain the broad spectrum of differentiation ability in MSCs.
Improved Short-Channel Characteristics With Long Data Retention Time in Extreme Short-Channel Flash Memory Devices (2016)

Owing to the scaling demands, source/drain (S/D) junction engineering has evolved as a promising technique to improve the performance and reliability of NAND flash memory devices. In this paper, we investigate the impact of S/D doping lateral straggle σL on the program characteristics, data retention, and short-channel effects (SCEs) for sub-25-nm NAND flash memory device. Here, we consider threshold voltage roll-off, subthreshold slope, and drain-induced barrier lowering parameters to study the SCE for the aforementioned memory device. We also examine the effect of varying σL on the junction boost leakage current [during the program-inhibition (P-I) mode] for the considered device. Based on our investigations, we have shown that adjusting the S/D doping lateral straggle σL appropriately not only improves SCE but also the program speed and data retention without any need of altering the gate oxide stack. Furthermore, the junction boost leakage current also decreases on reducing σL. Consequently, it enables the device to hold high channel potential during the P-I mode, and thereby reduces the risk of false programming/erasing of the device.
Novel fast-switching and high-data retention phase-change memory based on new Ga-Sb-Ge material (2015)

Attempts to improve the retention so far must sacrifice switching speed. This work explores new phase change material based on pseudobinary GaSb-Ge system. The resulting new phase-change material has demonstrated fast switching speed of 80 ns, long endurance of 1G cycles and excellent data retention that survives 250 oC-300 hrs. The 10 years-220 oC data retention is the best ever reported. It is also the fastest material that can pass the solder bonding criteria for embedded automotive applications.
Investigation of the Quantity of Exhaled Aerosols Released into the Environment during Nebulisation (2019)

The results highlight the potential secondary inhalation of exhaled aerosols from commercially available nebuliser facemask/mouthpiece combinations. The results will aid in developing approaches to inform policy and best practices for risk mitigation from fugitive emissions.
A Low Order Model for Vertical Axis Wind Turbines (2010)

A new computational model for initial sizing and performance prediction of vertical axis wind turbines is presented. The model uses a 2D hybrid dynamic vortex and blade element momentum approach. Each airfoil is modeled as a single vortex of time varying strength with an analytical model for the influence of the shed vorticity. The vortex strengths are calculated by imposing a flow tangency condition at the three-quarter chord location on each airfoil, modified in the case of stall. The total blade forces and the momentum-based streamtube deceleration are then obtained using pre-computed cd and cm 2D blade profile characteristics. Model fidelity is improved over previous models because flow c urvature, dynamic vortices, blade interactions, static stall, and streamtube changes are all taken into account. Fast convergence is obtained for a large range of solidity and tip speed ratio, which allows optimization of various parameters, including blade pitch angle variation.
Computer-Aided Design and Manufacturing of Customized Insoles (2011)

The authors developed a method for manufacturing customized insoles. It leverages advances in pressure analysis of the foot, including research on insole materials' mechanical properties and the design of insole components. Using a plan tar pressure measurement system and computer numerical control (CNC) or rapid-prototyping technology, it determines the distributed average pressure for each region of the foot, creates a model of the sole, and manufactures an insole. The method makes insole design much more precise, which enables rapid manufacturing and ensures more ergonomic and comfortable insoles.
Compression of Pulsed Terahertz Image Using Discrete Wavelet Transform (2007)

We present the result of data compression using discrete wavelet transform (DWT) in terahertz pulsed imaging (TPI). A test object composed of dielectric materials is imaged by a fast oscillating delay line in reflection geometry. In general, because the size of the original image is from several megabytes (MB) to tens of MB, an adequate compression algorithm that prevents the loss of relevant information is indispensable. We analyze the image by multilevel DWT and obtain the wavelet coefficients of the levels. The images reconstructed from some of the coefficients are compared with the original image. The reconstructed pulse signals and their frequency-domain spectrums for each pixel are compared with the original signals to obtain the optimum decomposition level. The image could be compressed below 15% of its original size without loss of image quality and significant errors in the time and frequency domains.
Initiation of mRNA Translation in Oncogenesis: The Role of eIF4E (2004)

The eukaryotic initiation factor 4E (eIF4E) is a key regulator of protein translation whose function is activated by the Akt and Ras proto-oncogenic signal transduction pathways. eIF4E enhances the translation of mRNAs encoding several genes involved in tumorigenesis and acts as a proto-oncogene, in vitro, when overexpressed in immortalized cells. Importantly, eIF4E is frequently found overexpressed in human cancers of multiple histological origins. However, in vivo evidence of the eIF4E neoplastic potential was lacking until now. Here we discuss recent findings that demonstrate eIF4E's oncogenic role in vivo through direct genetic approaches in the mouse, and identify novel oncogenic functions for this initiation factor in cooperative tumorigenesis and response to therapy.
Neospora species infection in a herd of dairy cattle (1996)

Congenital transmission of Neospora organisms together with the apparent lack of horizontal transmission observed in the herd reported here indicated that Neospora sp has the ability to be transmitted from dam to offspring for several generations. This mode of transmission would explain the maintenance of infection in a population of cattle despite the lack of a definitive host for the parasite.
Correlation of electron-transfer rate constants of carbanions with their oxidation potentials and basicities (1986)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCorrelation of electron-transfer rate constants of carbanions with their oxidation potentials and basicitiesFrederick G. Bordwell and Mark J. BauschCite this: J. Am. Chem. Soc. 1986, 108, 8, 1985–1988Publication Date (Print):April 1, 1986Publication History Published online1 May 2002Published inissue 1 April 1986https://pubs.acs.org/doi/10.1021/ja00268a042https://doi.org/10.1021/ja00268a042research-articleACS PublicationsRequest reuse permissionsArticle Views198Altmetric-Citations22LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Granulocyte-Colony Stimulating Factor (G-CSF) as an adjunct to antibiotics in the treatment of pneumonia in adults (2007)

There is no current evidence supporting the routine use of G-CSF in the treatment of pneumonia. Studies in which G-CSF is administered prophylactically or earlier in therapy may be of interest.
Dendritic Iodinated Contrast Agents with PEG-Cores for CT Imaging:  Synthesis and Preliminary Characterization (2006)

The purpose of this study was to design, synthesize, and initially characterize a representative set of novel constructs for large-molecular radiographic/computed tomography (CT) contrast agents, intended for a primarily intravascular distribution. A new assembly of well-known and biocompatible components consists of paired, symmetrical dendritic polylysines initiated from both ends of a poly(ethylene glycol) (PEG) core, yielding an array of multiple free amino groups to which were conjugated highly soluble and stable triiodophthalamide ("triiodo") moieties. An array of six dendritic contrast agents was synthesized originally, using three different PEG cores (3, 6, 12 kDa) with t-Boc lysine-generated dendrimer "amplifiers" (from three to five generations) containing 16 to 64 amino groups for conjugation with reactive triiodo moieties. A clinically used, nonionic, small molecular CT contrast agent, iobitridol, was derivatized via a hydroxyl protection/deprotection strategy, introducing a new carboxyl group available for conjugation to the lysine amino groups of dendrimers. Final products were purified by size exclusion chromatography and characterized by NMR, UV, HPLC, and elemental analysis. Preliminary evaluations were conducted for physicochemical characterization and in vivo CT contrast enhancement in a rat model. All six iodinated PEG-core dendrimer conjugates were synthesized in good yields, with a high degree of size monodispersity, large apparent molecular weight, favored physicochemical properties. A representative compound, PEG12000-carbamate-Gen4-IOB conjugate, 27% (w%) rich in iodine, demonstrated a desirable strong and persistent intravascular enhancement with a monoexponential blood half-life of approximately 35 min assayed by dynamic CT imaging and also showed high water solubility (>550 mg/mL at 25 degrees C), large apparent molecular size (comparable to a 143-kDa protein), high hydrophilicity (butanol-water partition coefficient 0.015), and stability to autoclaving conditions. This study showed the synthetic feasibility, desired basic characteristics, and potential utility for CT contrast enhancement achieved with a new type of iodinated, large-molecular PEG-core dendritic construct. Further development of this class of macromolecular contrast agents will be required to define the optimal formulation, pharmacology, safety profile, and the full range of diagnostic applications including tumor microvascular quantitative characterization by CT imaging.
Context-Dependent Logo Matching and Recognition (2012)

We contribute, through this paper, to the design of a novel variational framework able to match and recognize multiple instances of multiple reference logos in image archives. Reference logos and test images are seen as constellations of local features (interest points, regions, etc.) and matched by minimizing an energy function mixing: 1) a fidelity term that measures the quality of feature matching, 2) a neighborhood criterion that captures feature co-occurrence/geometry, and 3) a regularization term that controls the smoothness of the matching solution. We also introduce a detection/recognition procedure and study its theoretical consistency. Finally, we show the validity of our method through extensive experiments on the challenging MICC-Logos dataset. Our method overtakes, by 20%, baseline as well as state-of-the-art matching/recognition procedures.
Dynamic Routing and Frequency Slot Assignment for Elastic Optical Path Networks that Adopt Distance Adaptive Modulation (2011)

We propose a dynamic routing and frequency slot assignment algorithm for SLICE networks that employ distance adaptive modulation. We verify that the spectrum utilization penalty that stems from non-uniform bandwidth allocation is marginal.
Dynamic influences on static measures of metacognition (2022)

Humans differ in their capability to judge choice accuracy via confidence judgments. Popular signal detection theoretic measures of metacognition, such as M-ratio, do not consider the dynamics of decision making. This can be problematic if response caution is shifted to alter the tradeoff between speed and accuracy. Such shifts could induce unaccounted-for sources of variation in the assessment of metacognition. Instead, evidence accumulation frameworks consider decision making, including the computation of confidence, as a dynamic process unfolding over time. Using simulations, we show a relation between response caution and M-ratio. We then show the same pattern in human participants explicitly instructed to focus on speed or accuracy. Finally, this association between M-ratio and response caution is also present across four datasets without any reference towards speed. In contrast, when data are analyzed with a dynamic measure of metacognition, v-ratio, there is no effect of speed-accuracy tradeoff.
Comparison of Mucosal Microvasculature between the Proximal and Distal Human Colon (1996)

The microcirculation of the human colon with special reference to the differences in microvascular architecture between the proximal and distal colon was studied by scanning electron microscopy with vascular corrosion casting technique. The subsurface capillary networks of the ascending colon were honeycomb-like and multi-layered, with an average number of capillary layers per capillary loop of 3.28 +/- 1.10 (mean +/- SD). Whereas, those of the sigmoid colon were almost single-layered, and the average number of capillary layers was 1.19 +/- 0.39. In the cross-section of vascular casts of both parts of the colon, the ascending capillaries originating from the submucosal arterioles ascended into the mucosal layer and joined into the subsurface capillary networks, which drained into the collecting venules near the surface. The mean diameter of the collecting venules of the ascending and the sigmoid colon was 29.3 +/- 6.41 microns and 19.48 +/- 2.23 microns, respectively. From these findings, it is speculated that the multi-layered capillary networks of the proximal colon are closely related to the greater water absorption and electrolyte transport activities compared to those of the distal colon.
Cell-Free Synthesis and Affinity Isolation of Proteins on a Nanomole Scale (2000)

The performance of conventional cell-free gene expression systems based on the Escherichia coli S30 extract can be significantly improved by using expression vectors that encode viral structural elements known to enhance translation in vivo and to protect mRNA from ribonuclease action. The expression vectors reported here are designed to produce a functionally active protein carrying the Strep-tag oligopeptide at its C-terminus. They can be used in translation, transcription-translation or replication-translation reactions. Depending on its type, the reaction yields up to 40 micrograms per mL, or about 1 nmol of a standard protein. The presence of Strep-tag allows the synthesized protein to be easily isolated on a streptavidin-agarose column under mild conditions and the entire procedure to be completed within one working day. The results show that standard low-cost, cell-free systems can serve for rapid preparation of purified proteins in amounts that can satisfy a number of needs of a research laboratory.
Targeting of HER2 Antigen for the Treatment of Disseminated Peritoneal Disease (2004)

The studies reported herein demonstrate the efficacy of alpha-particle-targeted radiation therapy of peritoneal disease with Herceptin as the targeting vehicle. Using the CHX-A-DTPA linker, Herceptin was radiolabeled with indium-111 and bismuth-213 with high efficiency without compromising immunoreactivity. A pilot radioimmunotherapy study treating mice bearing 5-day LS-174T (i.p.) xenografts, a low but uniform HER2 expressing, human colon carcinoma, with a single dose of (213)Bi-CHX-A"-Herceptin, proved disappointing. This defined the effect of tumor burden/size on tumor response to radioimmunotherapy with alpha-radiation. A more successful experiment with a lower tumor burden (3 days) in mice followed. A specific dose-response (P = 0.009) was observed, and although a maximum-tolerated dose was not determined, a dose of 500 to 750 muCi was selected as the operating dose for future experiments based on changes in animal weight. Median survival was increased from 20.5 days for the mock-treated mice to 43 and 59 days with 500 and 750 muCi, respectively. The therapeutic effectiveness of (213)Bi-CHX-A"-Herceptin was also evaluated in a second animal model for peritoneal disease with a human pancreatic carcinoma (Shaw). The results of this study were not as dramatic as with the former model, and higher doses were required to obtain an increase in survival of the mice (P = 0.001).