gebhart/patrepeval huggingface dataset:
[Quantitative analysis on platelets cytosolic free calcium concentration and ulcerous tissue calcium in patients with recurrent aphthous ulceration]. (1998)

To study the relationship between the abnormal behavior of calcium (Ca2+) and the pathogenesis of recurrent aphthous ulceration (RAU) by measuring quantitatively the concentration of platelets' cytosolic free calcium ions ([Ca2+]i) and tissue calcium (Wca2+), the patients with RAU (n = 50) and the healthy volunteers (n = 30) were chosen randomly. Then platelets [Ca2+]i was determined in calcium fluorescent indicator Fura-2/AM loaded washed platelets by spectrofluorophotometer and tissue Wca2+ was measured quantitatively by OCPC autoanalyzer method. The results showed that the concentration levels of platelets [Ca2+]i in patients with RAU in attack stage and attack-free stage were significantly higher than that of the control group (P < 0.01, P < 0.05), and the concentration level of the ulcerous tissue Wca2+ was also obviously higher than that of the normal control group (P < 0.001). There was a significantly positive correlation between the platelets [Ca2+]i and the ulcerous tissue Wca2+ (P < 0.01). The results suggested that the influx disorder and overload of Ca2+ might play an important role in the pathogenesis of RAU.
Precise coil positioning system using multi-articular arm for location of stimulated brain area in transcranial magnetic stimulation (2009)

We developed a precise coil positioning system for determining stimulated sites in transcranial magnetic stimulation (TMS) by using a multi-articular mechanical arm. A figure-of-eight coil used in TMS can induce a focal electric field beneath the coils center in the cortex; therefore, the point on the cortex beneath the center of the figure-of-eight coil has been conventionally regarded as a stimulating site. However, a stimulating site determined in this manner is not believed to be plausible, because the electric field induced in TMS is dispersed over the brain vectorially. Our system was developed on the basis of the cortical structure of the brain cortex and the direction of the induced electric field. In order to verify the system accuracy, Motor Evoked Potentials (MEPs) elicited by TMS at the hand muscles innervated by the primary motor cortex (M1) were measured as electromyogram (EMG). And functional vectorial map of the excitability of M1 in the resting state was obtained by rotating the stimulation coil at a constant location.
Network acceleration technology based on peer-to-peer network (2010)

Most of the shared file system by P2P network is based on composite network frame, this frame integrate the intent and distributed P2P frame, it possess the advantage of both. How to download in a high speed is one direction of the research in shared file system. It researches and realizes the technology of acceleration based on transformed servers with cache under the frame of composite network frame. At the same time, it discuss the technology to avoid jitter buffer, and we take the relevant experiment to prove the technology presented in this paper is correct and valid at the end.
Characterization by Potentiometric Procedures of the Acid−Base and Metal Binding Properties of Two New Classes of Immobilized Metal Ion Affinity Adsorbents Developed for Protein Purification (1997)

The acid-base protonation constants of two recently introduced chelating ligands for protein purification, O-phosphoserine and 8-hydroxyquinoline immobilized onto Sepharose CL-4B, and the stability constants of their derived immobilized metal ion chelate complexes have been determined by potentiometric methods. The data confirm that immobilization thermodynamically constrains the ligands, with the electron-withdrawing characteristics of the group linking the ligand to the support material affecting the magnitude of the stability constant of the immobilized metal ion complex vis-à-vis the free ligand-metal ion complex in solution. The influence of buffer composition, ionic strength, and pH on the stability constant of the immobilized hard metal ion chelate complexes has also been examined. Collectively, the results have confirmed that coordination complexes with stoichiometries other than the simply 1:1 ML-type exist with these systems, with hard metal ions exhibiting a preference for hydrolytic M(OH)(m)L(n) complexes where m or n > 1. These findings on the participation of coordination complexes of different stoichiometry depending on the characteristics of the chelating ligand and metal ion have fundamental implications for the interpretation of immobilized metal ion affinity chromatographic separation of proteins.
Synthesis of Nickel Sulfides in Aqueous Solutions Using Sodium Dithionite (2000)

Reaction of nickel chloride with sodium dithionite in aqueous solutions at ambient temperature has been investigated systematically to obtain nickel sulfides. The products are characterized by X-ray diffraction, thermogravimetric analysis, and electrical resistivity and magnetic susceptibility measurements. It is found that the compositions and structures of the products are controlled by the reaction pH and the amount of the reactants. While reactions under highly acidic (pH < or = 2) and basic (pH > or = 7) conditions yield crystalline sulfur and amorphous or poorly crystalline NiySx, respectively, those at intermediate 3 < or = pH < or = 6 give crystalline NiySx. Both crystalline Ni3S2 (heazlewoodite structure) and Ni3S4 (spinel structure) have been obtained at room temperature. Additionally, NiS (millerite structure) is obtained by carefully heating Ni3S4 at 200 degrees C in a mixture of 90% Ar and 10% H2. Ni3S4 is found to be metastable, and it begins to disproportionate above 100 degrees C. Both Ni3S2 and Ni3S4 show metallic behavior. While Ni3S2 exhibits temperature-independent magnetic susceptibility, Ni3S4 shows ferrimagnetic ordering below 20 K.
Repeatability of Quantitative <sup>18</sup>F-NaF PET: A Multicenter Study (2016)

The repeatability of SUV<sub>max</sub>, SUV<sub>mean</sub>, and SUV<sub>total</sub> for <sup>18</sup>F-NaF PET/CT was similar between lesion- and patient-level ROIs. We found significant differences in lesion-level and patient-level distributions between sites. These results can be used to establish <sup>18</sup>F-NaF PET-based criteria for assessing treatment response at the lesion and patient levels. <sup>18</sup>F-NaF PET demonstrates repeatability levels useful for clinically quantifying the response of bone lesions to therapy.
Optimal surface and bulk passivation of high efficiency multicrystalline silicon solar cells (2002)

Conventional (CC) and electromagnetically cast (EMC) multicrystalline silicon solar cells are fabricated following different passivation schemes. Thin layers (/spl sim/100 /spl Aring/) of thermal dry and PECVD SiO/sub 2/ are implemented for providing oxide surface passivation for multicrystalline silicon solar cells. It is found that growing thin layers of thermal dry oxide results in efficient surface passivation. However, for thin PECVD SiO/sub 2/ layers it is necessary to perform, post deposition, low temperature (/spl sim/350/spl deg/C) forming gas anneal in order to observe the surface passivation effect. In addition, hydrogen plasma passivation has been optimized for achieving very deep penetration of atomic hydrogen in the material (>30 /spl mu/m) and as a consequence very effective bulk passivation of multicrystalline silicon solar cells. By combining the thermal dry surface oxide passivation with the hydrogen plasma treatment from the front and the back sides, efficiency of 17% on 4 cm/sup 2/ (independently confirmed by NREL as 16.93%) is realized without any Al gettering. On the other hand, the solar cell efficiencies obtained using thin layers of PECVD SiO/sub 2/ are found to be very comparable to the efficiency of the cells fabricated with thermal dry SiO/sub 2/ layers.
Identification of a Serum Gelatinase Associated With the Occurrence of Cerebral Aneurysms as Pro-Matrix Metalloproteinase-2 (1998)

These findings confirm that the increased gelatinolytic activity observed in vitro in a subset of cerebral aneurysm patients is due to pro-MMP-2.
Depression of glutamatergic transmission by nociceptin in the neonatal rat hemisected spinal cord preparation <i>in vitro</i> (1996)

The present study explored the action of nociceptin, the putative endogenous ligand for the orphan opioid receptor (ORL1), on the rat hemisected spinal cord preparation. Electrical stimulation of a dorsal root evokes a glutamatergic population ventral root potential (DR-VRP) in the corresponding ventral root. Low intensity stimulation evokes two A fibre-mediated components; a compound action potential of motoneurones superimposed on a population e.p.s.p. (excitatory postsynaptic potential); at higher stimulus intensities sufficient to activate C fibres a more prolonged population e.p.s.p. is evoked. All three components were depressed by nociceptin in a concentration-dependent manner with IC50 values (s.e.mean) of 119 +/- 2 nM (n = 4), 241 +/- 3 nM (n = 4) and 32 +/- 2 nM (n = 4), respectively. The depressant actions of nociceptin (30 nM and 300 nM) were not reversed by the opioid antagonist naloxone (1 microM). Nociceptin (100 nM and 300 nM) had no effect on the afferent volleys in the dorsal root. Nociceptin therefore appears to be acting as an inhibitory peptide at the spinal level through a naloxone-insensitive opioid receptor.
<b>A bHLH transcription factor mediates organ, region and flower type specific signals on dihydroflavonol‐4‐reductase (</b><i><b>dfr</b></i><b>) gene expression in the inflorescence of</b><i><b>Gerbera hybrida</b></i><b>(Asteraceae)</b> (1998)

The angiosperm family Asteraceae is characterized by composite inflorescences, which are highly organized structures consisting of different types of flowers. In order to approach the control of floral organ differentiation in Asteraceae at molecular level, we are studying regulation of flavonoid biosynthesis in Gerbera hybrida. Dihydroflavonol-4-reductase (dfr) expression is regulated according to anthocyanin pigmentation patterns in all tested gerbera varieties at several anatomical levels. We have isolated a promoter for one of the dfr genes, Pgdfr2. Gerbera plants transgenic for a Pgdfr2-uidA construct reveal that the activity of the Pgdfr2 promoter from one variety follows the pigmentation in other varieties which have different color patterns. It is thus evident that the observed complex regulation of dfr expression occurs in trans. In order to identify the trans-acting regulators, we isolated a cDNA (gmyc1) homologous to the previously characterized genes encoding bHLH-type regulators of the anthocyanin pathway in plants. The expression of gmyc1 in different varieties suggests that it has a major role in regulating dfr activity in corolla and carpel, but not in pappus and stamen. Specifically in gerbera, the identical patterns of gmyc1 and dfr expression in corolla tissue suggest that GMYC1 also regulates dfr expression in a region and flower type specific manner. Our studies show that in gerbera GMYC1-dfr interaction is part of several developmental processes characteristic for Asteraceae (such as specification of flower types across the composite inflorescence), whereas in other processes (such as differentiation of sepal as pappus) other regulators control dfr expression to determine the spatial specificity.
Streptokinase Promotes Development of Dipeptidyl Peptidase IV (CD26) Autoantibodies after Fibrinolytic Therapy in Myocardial Infarction Patients (2002)

Dipeptidyl peptidase IV (DPP IV) (CD26) plays a critical role in the modulation and expression of autoimmune and inflammatory diseases. We recently reported that sera from patients with rheumatoid arthritis and systemic lupus erythematosus contained low levels of DPP IV and high titers of anti-DPP IV autoantibodies of the immunoglobulin A (IgA) and IgG classes and found a correlation between the low circulating levels of DPP IV and the high titers of anti-DPP IV autoantibodies of the IgA class. Since streptokinase (SK) is a potent immunogen and binds to DPP IV, we speculated that patients with autoimmune diseases showed higher DPP IV autoantibody levels than healthy controls as a consequence of an abnormal immune stimulation triggered by SK released during streptococcal infections. We assessed this hypothesis in a group of patients suffering from acute myocardial infarction, without a chronic autoimmune disease, who received SK as part of therapeutic thrombolysis. Concomitant with the appearance of anti-SK antibodies, these patients developed anti-DPP IV autoantibodies. These autoantibodies bind to DPP IV in the region which is also recognized by SK, suggesting that an SK-induced immune response is responsible for the appearance of DPP IV autoantibodies. Furthermore, we determined a correlation between high titers of DPP IV autoantibodies and an augmented clearance of the enzyme from the circulation. Serum levels of the inflammatory cytokines tumor necrosis factor alpha (TNF-alpha) and interleukin 6 (IL-6) increased significantly after 30 days of SK administration, while the levels of soluble IL-2 receptor remained unchanged during the same period, suggesting a correlation between the lower levels of circulating DPP IV and higher levels of TNF-alpha and IL-6 in serum in these patients.
Comparation of the Flavor of Different Cheese Flavouring Agents Produced by Using Surface Ripening Bacterium and/or Enzymes (2013)

To accelerate cheese ripening, enhance its flavor types and intensity and make cheese flavoring agent in shorter time, surface ripening bacterium (<em>Brevibacterium linens</em> and <em>Debaryomyces hansenii</em>) and/or enzymes (<em>Flavorzyme</em> 500 MG and <em>Palatase</em> 20000 L) were used in cheese curd. In this study, aroma compounds generated by using ripening cultures and/or enzymes were analyzed. The control l was made by inoculating ripening cultures, while the control 2 was made through enzymes-modified only. Results showed that cheese flavoring agent made by using ripening strains in combination with enzymes had more volatile flavor compounds (at least 44) than that used just ripening bacterium (26) or just two enzymes (27). Then, through Solid-phase microextraction and Gas Chromatography-Mass Spectrometry analysis, we knew that sample 1, which was made through proteolysis first, next sprayed ripening cultures and last lipolysis, generated 54 flavor compounds. Sample 2, which enzymed cheese curd first, then incubated ripening cultures, had 44 aroma compounds. However, the controls 1, incubated ripening strains only, had 26 volatile compounds, while the control 2, enzymed only, had 27 volatile compounds. This study reveals that ripening bacterium could contribute more to the generation of acids, sulphur compounds, miscellaneous compounds and alcohols, it has a good potential to be used in cheese flavoring agents making. Besides, the combination of surface strains and enzymes, especially using Flavorzyme 500 MG first, then sprayed ripening cultures and at last Palatase 20000 L, could get more volatile compounds.
Foliar Sprays of Citric Acid and Malic Acid Modify Growth, Flowering, and Root to Shoot Ratio of<i>Gazania</i>(<i>Gazania rigens</i>L.): A Comparative Analysis by ANOVA and Structural Equations Modeling (2014)

Foliar application of two levels of citric acid and malic acid (100 or 300 mg L −1 ) was investigated on flower stem height, plant height, flower performance and yield indices (fresh yield, dry yield and root to shoot ratio) of Gazania . Distilled water was applied as control treatment. Multivariate analysis revealed that while the experimental treatments had no significant effect on fresh weight and the flower count, the plant dry weight was significantly increased by 300 mg L −1 malic acid. Citric acid at 100 and 300 mg L −1 and 300 mg L −1 malic acid increased the root fresh weight significantly. Both the plant height and peduncle length were significantly increased in all applied levels of citric acid and malic acid. The display time of flowers on the plant increased in all treatments compared to control treatment. The root to shoot ratio was increased significantly in 300 mg L −1 citric acid compared to all other treatments. These findings confirm earlier reports that citric acid and malic acid as environmentally sound chemicals are effective on various aspects of growth and development of crops. Structural equations modeling is used in parallel to ANOVA to conclude the factor effects and the possible path of effects.
The Most Stable Protein–Ligand Complex: Applications for One‐Step Affinity Purification and Identification of Protein Assemblies (2012)

An alternative to tagging: The thermodynamically most stable protein–ligand complex known to date (KD=1.5×10−20 M) consists of the two components FimGt and DsF, portions of two different subunits of the type 1 pilus protein complex. The first technical applications of this new complex are the single-step purification and identification of multiprotein complexes from cell extracts. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Challenging Industrialization: The Rekindling of Agrarian Protest in a Modern Agriculture, 1977–1987 (1993)

This analysis explores why a farmers-led social movement mobilized against federal government policy in the late twentieth century. It also explores where that revolt may lead and whether it was different from previous farm protests. Was it, as populist rhetoric of the 1980s charged, a reflection of structural changes in farmers' own agricultural production systems? Or was it simply a case of farmers wanting more federal income support? The distinction is important because the answer determines producer commitment to the central agricultural development premises of U.S. public policy. Farmers always have promoted the agrarian value of hardworking independence. Yet they also have been caught, especially since midcentury, in a cycle of farm industrialization. Technical innovation and federal agricultural policy have combined to make industrialization unavoidable for individual farm operators who want to remain as full-time, commercial growers. Has there been a link between the dissenting politics of farm protest and industrialized agricultural change? This linkage is the subject of concern in the following pages. Why industrialization might well have caused policy dissent is examined first.
Feasibility of using a low-cost inertial measurement unit with centimeter accuracy differential global positioning system (1999)

A, (vector) T h [I] (matrix) \V (vector) (PC 6cp (vector) 60 (vector) Variable Name Gravitation vector error due to error in assumed position (rn/sec2) Gravitation deflection and anomaly (m/sec2) Accelerometer errors in the navigation frame (m/sec2) Gyro errors in the navigation frame (deglsec) Resolution Threshold Vehicle Inertia Matrix PSI-Error Angle (deg) Residual Error (deg) Attitude error indicating the extent of platform misalignment (deg) Error in the attitude due to the computed position error (deg)
lncRNA <i>PVT1</i> Promotes Metastasis of Non-Small Cell Lung Cancer Through EZH2-Mediated Activation of Hippo/NOTCH1 Signaling Pathways. (2021)

These results suggested that lncRNA <i>PVT1</i> promotes NSCLC metastasis through EZH2-mediated activation of Hippo/NOTCH1 signaling pathways. This study provides a new opportunity to advance our understanding in the potential mechanism of NSCLC development.
Left Ventricular Function in Acute Myocardial Infarction Evaluated by Gated Scintiphotography (1974)

Ten normal volunteers and 38 patients with acute myocardial infarction were evaluated by biplane gated blood pool scanning. The mean left ventricular end-diastolic volume in those with infarction was 125 ± 41 ml/m 2 compared to 82 ± 10 ml/m 2 in the normals. The left ventricular end-systolic volume was 82 ± 35 ml/m 2 compared to 35 ± 4 ml/m 2 , and the left ventricular ejection fraction 36 ± 8% compared to 56 ± 3% in the normals. Thirty-six of the 38 patients with infarction had an area of akinesis which ranged from 15 to 59% of the left ventricular wall. Patients with acute myocardial infarction were found to have a significant increase in left ventricular end-systolic volume and decrease in ejection fraction compared to normals. The end-diastolic volume was, however, increased only in those with an elevated left ventricular filling pressure or decreased cardiac index. Follow-up studies obtained in 20 patients between one week and three months following infarction showed that in the 14 who improved clinically, left ventricular ejection fraction significantly increased from 38 to 45% ( P < 0.001) while in six who failed to show clinical improvement or worsened, left ventricular ejection fraction remained at 30%. Left ventricular ejection fraction was significantly greater and the extent of akinesis significantly less in the patients who survived compared to those who died.
Type II fatty acid synthesis is essential only for malaria parasite late liver stage development (2008)

Intracellular malaria parasites require lipids for growth and replication. They possess a prokaryotic type II fatty acid synthesis (FAS II) pathway that localizes to the apicoplast plastid organelle and is assumed to be necessary for pathogenic blood stage replication. However, the importance of FAS II throughout the complex parasite life cycle remains unknown. We show in a rodent malaria model that FAS II enzymes localize to the sporozoite and liver stage apicoplast. Targeted deletion of FabB/F, a critical enzyme in fatty acid synthesis, did not affect parasite blood stage replication, mosquito stage development and initial infection in the liver. This was confirmed by knockout of FabZ, another critical FAS II enzyme. However, FAS II-deficient Plasmodium yoelii liver stages failed to form exo-erythrocytic merozoites, the invasive stage that first initiates blood stage infection. Furthermore, deletion of FabI in the human malaria parasite Plasmodium falciparum did not show a reduction in asexual blood stage replication in vitro. Malaria parasites therefore depend on the intrinsic FAS II pathway only at one specific life cycle transition point, from liver to blood.