gebhart/patrepeval huggingface dataset:
Use of a synthetic homologue of human fibrinopeptide A for production of a monoclonal antibody specific for the free peptide (1989)

Abstract It has been shown that epitopes reactive with one group of rabbit antibodies to human fibrinopeptide A (hFPA, A alpha 1–16) are included in its COOH-terminal region (A alpha 7–16). It was further established that Asp-7, Phe-8, and Arg-16 contribute to immunoreactivity and that intact fibrinogen and hFPA-containing fragments react poorly with such antibodies. The purpose of this investigation was to prepare a synthetic peptide corresponding to A alpha 7–16 and use it for generation of FPA-specific monoclonal antibodies (MoAbs). Such probes would allow for development of assays that could measure hFPA directly in plasma. In our approach, an ovalbumin-conjugate of the hFPA homologue served as immunogen. Mouse spleen cells were fused with the immunoglobulin nonsecretor myeloma (P3X63Ag8.653). A hybridoma (8C2–5) has been isolated that secretes an antibody (MoAb/8C2–5) with the following characteristics: (a) IgG1, kappa isotype; (b) equilibrium dissociation constant of 1.5 +/- 0.2 x 10(7) L/mol with the [125I]- labeled N-tyrosyl derivative of hFPA [( 125I] Tyr-hFPA) as ligand; (c) reacts with hFPA and dog FPA (dFPA) but not with the des Arg (A alpha 1– 15) or shorter peptides; (d) does not react with intact fibrinogen or A alpha-chain of human or dog origin; (e) does not react with the elastase-generated hFPA-containing peptide A alpha 1–21. Enzyme-based immunoassays (EIAs) have been developed for measuring plasma hFPA levels in the range 3 x 10(-8) to 5 x 10(-7) mol/L. Since it has already been shown by a number of investigators that hFPA levels in patients with overt defibrination fall into this range, we propose that the MoAb/8C2–5-based assays may serve as useful clinical tools in screening patients at risk of thrombosis. The 8C2–5 antibody may also be helpful in studies dealing with congenital dysfibrinogenemias, particularly in identifying heterozygous propositi with amino acid substitutions at any position within the A alpha 7–16 region. Finally, due to its cross-reactivity with dFPA, assays using this antibody should also be valuable in the canine experimental thrombosis model studies.
Multistable Shape-Shifting Surfaces (2012)

This paper presents designs for Multistable Shape-Shifting Surfaces (MSSS) by introducing bistability into the Shape-Shifting Surface (SSS). SSSs are defined as surfaces that retain their effectiveness as a physical barrier while undergoing changes in shape. The addition of bistability to the SSS gives the surface multiple distinct positions in which it remains when shifted to, i.e. by designing bistability into a single SSS link, the SSS unit cell can change into multiple shapes, and stabilize within the resulting shape, while maintaining integrity against various forms of external assaults normal to its surface. Planar stable configurations of the unit cell include, expanded, compressed, sheared, half-compressed, and partially-compressed, resulting in the planar shapes of a large square, small square, rhombus, rectangle, and trapezoid respectively. Tiling methods were introduced which gave the ability to produce out-of-plane assemblies using planar MSSS unit cells. Applications for MSSSs include size-changing vehicle beds, expandable laptop screens, deformable walls, and volume-changing rigid-storage containers. Analysis of the MSSS was done using Pseudo-rigid-Body Models (PRBMs) and Finite Element Analysis (FEA) which ensured bistable characteristics before prototypes were fabricated.
Modeling of multiple scattering among vias in planar waveguides using Foldy–Lax equations (2001)

Abstract The full‐wave solution of multiple scattering among cylindrical vias in planar waveguides is modeled by using Foldy–Lax equations. By using the equivalence principle, the coupling among traces with many vias is decomposed into interior and exterior problems. For the interior problem, the dyadic Green's function is expressed in terms of vector cylindrical waves and waveguide modes. The Foldy–Lax equations of multiple scattering among the cylindrical vias are calculated. The waveguide modes are decoupled in the Foldy–Lax equations. The scattering matrix of coupling among vias is calculated. Numerical simulations of the scattering matrix are illustrated for several hundred vias. © 2001 John Wiley & Sons, Inc. Microwave Opt Technol Lett 31: 201–208, 2001.
Assessing the Impact of Corneal Refraction and Iris Tissue Non-Planarity on Iris Recognition (2018)

Assumptions regarding eye morphology are implicit in iris recognition algorithms. The cornea is assumed to have little to no effect on the view of the iris texture when the eye gaze is non-frontal, the iris is assumed flat, and the eye is assumed to be imaged via an orthographic projection. If these assumptions hold, affine transformations may be used to rectify non-frontally posed images to a frontal view and the rubber sheet model may be accurately used to normalize the iris annulus to a rectangular image. This paper examines how iris recognition performance degrades when the first two assumptions are violated. Using a computer renderable eye model, a data set is created varying the presence of the cornea and a parameterized non-planarity of the iris shape across a large range of eye gaze angles. Matching scores are created using a commercial matcher. When comparing the relative impact of each assumption violation, it is observed that iris non-planarity presents a more significant problem than corneal refractive distortion with regard to iris recognition accuracy in non-frontal images.
Lithography-Free Formation of Nanopores in Plastic Membranes Using Laser Heating (2006)

Synthetic nanopores are a new class of single-molecule sensors capable of electronically detecting, counting, and characterizing biomolecules. There have been studies of nanopore formation in solid-state materials. This paper reports a novel lithography-free method of nanopore formation in plastic membranes fluidized using laser heating. It was found that the pore shrinking dynamics follows a universal behavior with the diameter of a pore decreasing linearly with time similar to that found in fluidized SiO(2). A theoretical model based on a surface-tension-driven mass flow mechanism is proposed to successfully explain the observed universality in the pore shrinking dynamics. We demonstrate the potential of this lithography-free nanofabrication technique in biomolecular sensing with a lambda-DNA detection experiment.
Identification and Functional Characterization of a Voltage-gated Chloride Channel and Its Novel Splice Variant in Taste Bud Cells (2005)

Taste bud cells are epithelial cells with neuronal properties. Voltage-dependent ion channels have been physiologically described in these cells. Here, we report the molecular identification and functional characterization of a voltage-gated chloride channel (ClC-4) and its novel splice variant (ClC-4A) from taste bud cells. ClC-4A skipped an exon near its 5'-end, incurring the loss of 60 amino acids at the N terminus. In situ hybridization and immunohistochemistry localized these two channels' transcripts and proteins to a subset of taste bud cells. Electrophysiological recordings of the heterologously expressed channels in Xenopus oocytes showed that ClC-4 and ClC-4A have opposite sensitivity to pH and unique ion selectivity. The chloride channel blockers niflumic acid and 5-nitro-2-(3-phenylpropylamino)benzoic acid had a slight or no inhibitory effect on the conductance of ClC-4, but both blockers inhibited ClC-4A, suggesting that ClC-4A is a candidate channel for an acid-induced 5-nitro-2-(3-phenylpropylamino)benzoic acid-sensitive current. Furthermore, these two channels may play a role in bitter-, sweet-, and umami-mediated taste transmission by regulating transmitter uptake into synaptic vesicles.
Molecular Cloning of Mouse and Bovine Chondromodulin-II cDNAs and the Growth-Promoting Actions of Bovine Recombinant Protein (1999)

We previously determined the complete primary sequence of a heparin-binding growth-promoting factor, chondromodulin-II (ChM-II), which stimulated the growth of chondrocytes and osteoblasts in culture. Bovine ChM-II was a 16-kDa basic protein with 133 amino acid residues and exhibited a significant sequence similarity to the repeats of the chicken mim-1 gene product. Here we report the nucleotide sequences of bovine and mouse ChM-II cDNAs. The cDNAs each contained an open-reading frame corresponding to the ChM-II precursor with 151 amino acid residues. The N-terminus of the precursor included a secretory signal sequence of 18 amino acids prior to the mature ChM-II sequence. Unlike MIM-1, there was no repeat structure in the precursor protein, indicating that ChM-II was encoded as a gene product distinct from MIM-1. We then expressed recombinant bovine ChM-II protein which was purified to homogeneity. The recombinant protein stimulated the growth of rabbit growth plate chondrocytes, mouse MC3T3-E1 cells and rat UMR-106 osteoblastic cells in vitro.
Functional fullerenes for organic photovoltaics (2012)

Tremendous progress has been made on the design and processing of new active and interfacial materials to enable organic photovoltaics to achieve high power conversion efficiencies of >10%. In this Feature Article the development of functional fullerenes as (1) acceptors, (2) electron selective layers, and (3) morphology stabilizers for bulk heterojunction polymer solar cells is reviewed. In addition to the standard PCBM based acceptors, a wide variety of newly developed fullerene-derived molecules have appeared in the past few years and started to show very encouraging photovoltaic performance when they were blended with low bandgap conjugated polymers. New fullerene derivatives with proper molecular design can also serve as electron selective interfacial materials and morphology stabilizers for the bulk heterojunction layer, which are essential to improve the interfacial property and long term stability of polymer solar cells. Although there still are many challenges ahead before practical polymer solar cells will arrive in the market place, the research in functional fullerenes deserves to have more attention in order to expedite this development process.
Better knowledge management through knowledge engineering (2001)

The authors believe that current knowledge management practice significantly under-utilizes knowledge engineering technology, despite recent efforts to promote its use. They focus on two knowledge engineering processes: using knowledge acquisition processes to capture structured knowledge systematically; and using knowledge representation technology to store the knowledge, preserving important relationships that are far richer than those possible in conventional databases. To demonstrate the usefulness of these processes, we present a case study in which the drilling optimization group of a large oil and gas service company uses knowledge engineering practices to support the three facets of the knowledge management task: knowledge capture; knowledge storage; and knowledge deployment.
Efficient Multi-Material Topology Optimization (2021)

This chapter focuses on generating optimized topologies using multiple materials. The interest in multi-material topology optimization (MMTO) stems from the well-recognized synergy between topology optimization (TO) and additive manufacturing (AM), where organic structures created through TO can be directly fabricated by a variety of AM processes. Given the rapidly increasing capabilities of AM, there is an opportunity to improve the performance of consumer products, biomedical, and aerospace components, through simultaneous optimization of topology and distribution of multiple materials.
Sound absorbing diffusor (1990)

Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter Facebook Reddit LinkedIn Tools Icon Tools Reprints and Permissions Cite Icon Cite Search Site Citation Peter D’Antonio, John H. Konnert; Sound absorbing diffusor. J. Acoust. Soc. Am. 1 February 1990; 87 (2): 924. https://doi.org/10.1121/1.398882 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAcoustical Society of AmericaThe Journal of the Acoustical Society of America Search Advanced Search |Citation Search
A comparison of drying operations on the rheological properties of whey protein thickening ingredients (2004)

Abstract An existing procedure for the alteration of whey proteins into a cold-set thickening agent was modified by developing a spray-drying operation to replace the prohibitively expensive freeze-drying step. The original and the modified derivatization procedures were used with a commercial whey protein concentrate (WPC). The freeze-dried and spray-dried derivatized WPC powders, along with polysaccharide thickeners, were reconstituted in water and evaluated by using a range of rheological studies. The effects of temperature, concentration, and shear on viscosity as well as the mechanical spectra were assessed to characterize the ability of the powders to function in food systems. Rheological characterization revealed the modified derivatization procedure yielded an ingredient having the same cold-set thickening and gelling ability as the original derivatized powder. The modified whey proteins were also able to achieve, at higher usage levels, textural properties similar to several polysaccharide thickeners. Use of a spray-drying technique created a more economical process for the production of a whey protein ingredient that was suitable for contributing viscosity and texture to a wide range of food systems.
Perspectives in camptothecin development (2002)

DNA topoisomerase I is recognised as a useful target for antitumour therapy. Camptothecin is the prototype topoisomerase I specific inhibitor. The impressive antitumour activity of camptothecins in preclinical models and the clinical success of topotecan and irinotecan have stimulated intense research activities and various strategies to identify novel compounds and to overcome the main limitations of the camptothecin molecule (i.e., instability of the lactone ring and reversibility of drug–target interaction). The original water-insolubility problem of the naturally occurring compound has been overcome with the development of various water-soluble analogues, for intravenous administration. Some of these compounds fulfilled requirements for clinical development. The approaches to improve the antitumour efficacy of agents of this class rely on quite different principles. Some involve the development of prodrugs and delivery systems. Recently, novel series of lipophilic analogues have been described. These share some common features, including oral bioavailability, ability to cross the blood–brain barrier, an increased stability of the lactone ring and potent topoisomerase I inhibition. Several derivatives are still in preclinical development and some of these novel agents are presently undergoing clinical evaluation. This review will focus on the progress currently being made on various approaches in this field.
P‐196L: <i>Late‐News Poster</i> : High‐Performance Zinc Oxide Transistors by an Ambient Process (2007)

Abstract An ambient process was developed for producing both the dielectric and semiconductor layers of a zinc oxide‐based thin‐film transistor. The dielectric films exhibited good electrical properties, leading to devices with gate leakage less than 25 nA/cm 2 . Typical devices had mobilities in excess of 5 cm 2 /Vs from a process with a maximum substrate temperature of 200°C. In addition, devices showed reasonable electrical stability in this novel deposition process for dielectrics and semiconductors.
Automated Measurement of the Arteriolar-to-Venular Width Ratio in Digital Color Fundus Photographs (2011)

A decreased ratio of the width of retinal arteries to veins [arteriolar-to-venular diameter ratio (AVR)], is well established as predictive of cerebral atrophy, stroke and other cardiovascular events in adults. Tortuous and dilated arteries and veins, as well as decreased AVR are also markers for plus disease in retinopathy of prematurity. This work presents an automated method to estimate the AVR in retinal color images by detecting the location of the optic disc, determining an appropriate region of interest (ROI), classifying vessels as arteries or veins, estimating vessel widths, and calculating the AVR. After vessel segmentation and vessel width determination, the optic disc is located and the system eliminates all vessels outside the AVR measurement ROI. A skeletonization operation is applied to the remaining vessels after which vessel crossings and bifurcation points are removed, leaving a set of vessel segments consisting of only vessel centerline pixels. Features are extracted from each centerline pixel in order to assign these a soft label indicating the likelihood that the pixel is part of a vein. As all centerline pixels in a connected vessel segment should be the same type, the median soft label is assigned to each centerline pixel in the segment. Next, artery vein pairs are matched using an iterative algorithm, and the widths of the vessels are used to calculate the AVR. We trained and tested the algorithm on a set of 65 high resolution digital color fundus photographs using a reference standard that indicates for each major vessel in the image whether it is an artery or vein. We compared the AVR values produced by our system with those determined by a semi-automated reference system. We obtained a mean unsigned error of 0.06 (SD 0.04) in 40 images with a mean AVR of 0.67. A second observer using the semi-automated system obtained the same mean unsigned error of 0.06 (SD 0.05) on the set of images with a mean AVR of 0.66. The testing data and reference standard used in this study has been made publicly available.
Retroviral Gene Transfer Using Safe and Efficient Packaging Cell Lines (1990)

One of the requirements for the use of retroviral vectors in human gene therapy is a packaging cell line which is incapable of producing replication-competent virus and which produces high titers of replication-deficient vector virus. Wild-type virus may be produced through recombinational events between the helper virus and a retroviral vector. We have constructed an ecotropic packaging cell line, GP + E-86, and an amphotropic packaging cell line, GP + envAm12, in which the viral gag and pol genes are on one plasmid and the viral env gene is on another plasmid. Both plasmids contain deletions of the packaging sequence and the 3' LTR. The fragmented helper virus genomes, when introduced into 3T3 cells, produce titers of retrovirus which are comparable to the titers produced from packaging cells containing the helper virus genome on a single plasmid. We have found no evidence for the generation of wild-type retrovirus using the GP + E-86 and GP + envAm12 packaging lines, either alone or in combination with the N2 retroviral vector. We also show that these packaging cell lines can be used to transfer the neoR gene of the N2 vector into mouse hematopoietic cells, followed by successful (48-52%), long-term (up to 200 days) transplantation into irradiated recipients. These results indicate that these packaging lines are safe and efficient for use in experiments designed for murine (using GP + E-86) and human (using GP + envAm12) gene therapy.
Herpes Simplex Virus 1 Mutant with Point Mutations in <i>UL39</i> Is Impaired for Acute Viral Replication in Mice, Establishment of Latency, and Explant-Induced Reactivation (2018)

In the process of generating herpes simplex virus 1 (HSV-1) mutations in the viral regulatory gene encoding infected cell protein 0 (ICP0), we isolated a viral mutant, termed KOS-NA, that was severely impaired for acute replication in the eyes and trigeminal ganglia (TG) of mice, defective in establishing a latent infection, and reactivated poorly from explanted TG. To identify the secondary mutation(s) responsible for the impaired phenotypes of this mutant, we sequenced the KOS-NA genome and noted that it contained two nonsynonymous mutations in <i>UL39</i>, which encodes the large subunit of ribonucleotide reductase, ICP6. These mutations resulted in lysine-to-proline (residue 393) and arginine-to-histidine (residue 950) substitutions in ICP6. To determine whether alteration of these amino acids was responsible for the KOS-NA phenotypes <i>in vivo</i>, we recombined the wild-type UL39 gene into the KOS-NA genome and rescued its acute replication phenotypes in mice. To further establish the role of <i>UL39</i> in KOS-NA's decreased pathogenicity, the <i>UL39</i> mutations were recombined into HSV-1 (generating UL39<sup>mut</sup>), and this mutant virus showed reduced ocular and TG replication in mice comparable to that of KOS-NA. Interestingly, ICP6 protein levels were reduced in KOS-NA-infected cells relative to the wild-type protein. Moreover, we observed that KOS-NA does not counteract caspase 8-induced apoptosis, unlike wild-type strain KOS. Based on alignment studies with other HSV-1 ICP6 homologs, our data suggest that amino acid 950 of ICP6 likely plays an important role in ICP6 accumulation and inhibition of apoptosis, consequently impairing HSV-1 pathogenesis in a mouse model of HSV-1 infection.<b>IMPORTANCE</b> HSV-1 is a major human pathogen that infects ∼80% of the human population and can be life threatening to infected neonates or immunocompromised individuals. Effective therapies for treatment of recurrent HSV-1 infections are limited, which emphasizes a critical need to understand in greater detail the events that modulate HSV-1 replication and pathogenesis. In the current study, we identified a neuroattenuated HSV-1 mutant (i.e., KOS-NA) that contains novel mutations in the UL39 gene, which codes for the large subunit of ribonucleotide reductase (also known as ICP6). This mutant form of ICP6 was responsible for the attenuation of KOS-NA <i>in vivo</i> and resulted in diminished ICP6 protein levels and antiapoptotic effect. Thus, we have determined that subtle alteration of the UL39 gene regulates expression and functions of ICP6 and severely impacts HSV-1 pathogenesis, potentially making KOS-NA a promising vaccine candidate against HSV-1.