gebhart/patrepeval huggingface dataset:
MiR-24 Tumor Suppressor Activity Is Regulated Independent of p53 and through a Target Site Polymorphism (2009)

MicroRNAs (miRNAs) are predicted to regulate approximately 30% of all human genes; however, only a few miRNAs have been assigned their targets and specific functions. Here we demonstrate that miR-24, a ubiquitously expressed miRNA, has an anti-proliferative effect independent of p53 function. Cell lines with differential p53 status were used as a model to study the effects of miR-24 on cell proliferation, cell cycle control, gene regulation and cellular transformation. Overexpression of miR-24 in six different cell lines, independent of p53 function, inhibited cell proliferation and resulted in G2/S cell cycle arrest. MiR-24 over expression in cells with wt-p53 upregulated TP53 and p21 protein; however, in p53-null cells miR-24 still induced cell cycle arrest without the involvement of p21. We show that miR-24 regulates p53-independent cellular proliferation by regulating an S-phase enzyme, dihydrofolate reductase (DHFR) a target of the chemotherapeutic drug methotrexate (MTX). Of interest, we found that a miR-24 target site polymorphism in DHFR 3' UTR that results in loss of miR-24-function and high DHFR levels in the cell imparts a growth advantage to immortalized cells and induces neoplastic transformation. Of clinical significance, we found that miR-24 is deregulated in human colorectal cancer tumors and a subset of tumors has reduced levels of miR-24. A novel function for miR-24 as a p53-independent cell cycle inhibitory miRNA is proposed.
Joining of stainless-steel specimens with nanostructured Al/Ni foils (2003)

We describe the joining of stainless-steel specimens at room temperature using free-standing Al/Ni foils as local heat sources for melting AuSn solder layers. The foils contain many nanoscale layers of Al and Ni that react exothermically, generating a self-propagating reaction. The heats, velocities, and products of the reactions are described, and the microstructure and the mechanical properties of the resulting joints are characterized. Increasing the foil thickness, and thereby increasing the total heat released, can improve the strength of the joints until foil thickness reaches 40 μm. For thicker foils, the shear strength is almost constant at 48 MPa, compared to 38 MPa for conventional solder joints. The higher strength is due to finer microstructures in the solder layers of reactive joints. A numerical study of heat transfer during reactive joining and experimental results suggest that the solder layers need to melt completely and remain molten for at least 0.5 ms to form a strong joint.
Structure-activity relationship of cyclic imide herbicides. I. N-Substituted phenyl-3,4,5,6-tetrahydrophthalimides and related compounds. (1976)

In the course of the development of new herbicide (MK-616: N-(p-chlorophenyl)-3, 4, 5, 6-tetrahydrophthalimide), the structure-activity relationship of cyclic imide herbicides was investigated. Sixty N-substituted phenyl-3, 4, 5, 6-tetrahydrophthalimides and related compounds were prepared and their herbicidal activity were examined. The results indicate that, for high inhibitory activity, lipophilic tetramethylene moiety, double bond or nitrogen atom (s) in bridgehead position and N-(p-substituted) phenyl moiety are essential in these cyclic imides. New 4-(p-chlorophenyl)-1, 2-tetramethyleneurazole and 3 (p-chlorophenyl)-1, 5-tetramethylenehydantoin were found to be herbicidally active.
Importance of Phenolic Address Groups in Opioid Kappa Receptor Selective Antagonists (2004)

In vitro characterization and comparison of JDTic, its dehydroxy analogue and nor-BNI, and its dehydroxy analogue demonstrates that the N-substituted 3,4-dimethyl-(3-hydroxyphenyl)piperidine-derived antagonist, JDTic, relies more heavily on its phenol address group for affinity and antagonist activity relative to the corresponding naltrexone derived antagonists, nor-BNI. The structural flexibility of the former class of compound relative to the latter is postulated to underlie the difference.
Effects of modified tall oil versus a commercial source of conjugated linoleic acid and increasing levels of modified tall oil on growth performance and carcass characteristics of growing-finishing pigs. (2000)

Two experiments were conducted to evaluate the effects of conjugated linoleic acid (CLA)-enriched feed additives for swine. These additives included a source of CLA that was commercially available (CLA-60) and modified tall oil (MTO). Experiment 1 used 36 barrows (initially 37.6+/-2.8 kg) to compare the effects of CLA-60 and MTO on growth performance and carcass characteristics of finishing pigs. The corn-soybean meal diets contained .50% soybean oil (control), .50% CLA-60, or .50% MTO. Pigs fed CLA-60 had less (P = .03) ADG from 37.6 to 72.6 kg than the control pigs; otherwise, pigs fed either CLA-60 or MTO had growth performance similar (P > .15) to that of the control pigs. Pigs fed MTO grew faster (P = .03) and consumed more feed (P = .10) over the duration of the experiment (37.6 to 106.4 kg) than pigs fed CLA-60. Dietary treatment did not affect (P > .15) plasma triglycerides or carcass characteristics, but pigs fed either MTO or CLA-60 had greater saturation of fatty acids in the adipose tissue at the 10th rib than pigs fed the control diet. Experiment 2 used 80 barrows (initially 33.4+/-2.2 kg) to examine the effects of increasing levels of MTO on growth performance and carcass characteristics of finishing pigs. The corn-soybean meal diet contained 1% cornstarch, which was replaced with MTO to give dietary levels of .25, .50, or 1.00% MTO. Dietary treatment did not affect (P > .15) growth performance. Feeding increasing levels of MTO quadratically decreased (P = .02) average backfat thickness and longissimus muscle drip loss (P = .04) and quadratically increased longissimus muscle area (P = .07) and percentage lean (P = .03). Feeding MTO tended to increase belly firmness (P < .10) compared with pigs fed the control diet. These traits appeared to be optimized with .50% MTO. In summary, pigs fed MTO had greater ADG, ADFI, and ending BW than pigs fed CLA-60. Feeding MTO does not appear to affect growth performance but improves carcass lean content and may additionally improve some aspects of meat quality in growing-finishing pigs.
Epitope mapping of antibodies against ferritin heavy chain in giant cell arteritis and polymyalgia rheumatica (2013)

The potential diagnostic test for GCA/PMR can be improved by combining three human ferritin peptide antibodies.
Polarization-independent waveguide grating coupler using an optimized polysilicon overlay (2022)

We propose and validate a new, to the best of our knowledge, approach to designing a polarization-independent waveguide grating coupler, using an optimized polysilicon overlay on a silicon grating structure. Simulations predicted coupling efficiencies of about -3.6 dB and -3.5 dB for TE and TM polarizations, respectively. The devices were fabricated using photolithography in a multi-project wafer fabrication service by a commercial foundry and have measured coupling losses of -3.96 dB for TE polarization and -3.93 dB for TM polarization.
mRNA Profiling for Body Fluid Identification by Multiplex Quantitative RT‐PCR* (2007)

An alternative approach to conventional protein-based body fluid identification is gene expression profiling analysis. In the present work, we report the development of sensitive and robust multiplex quantitative reverse transcriptase-PCR assays for the identification of blood, saliva, semen, and menstrual blood. Each body fluid assay comprises a triplex system that detects transcripts from two body fluid-specific genes and a housekeeping gene GAPDH. The body fluid-specific genes include erythroid delta-aminolevulinate synthase (ALAS2) and beta-spectrin (SPTB) for blood, statherin (STATH) and histatin 3 (HTN3) for saliva, protamine 1 (PRM1) and protamine 2 (PRM2) for semen, and matrix metalloproteinase 7 (MMP7) and matrix metalloproteinase 10 (MMP10) for menstrual blood. Normalization of both body fluid-specific genes to the housekeeping gene by means of appropriate cycle threshold metrics ensures the high specificity of each assay for its cognate body fluid.
Improved Donors for the Separation of the Boron Isotopes by Gas-Liquid Exchange Reactions (1994)

Abstract Chemical exchange between gaseous boron trifluoride (BF3) and the liquid BF3·dimethyl ether complex has been used extensively for the commercial fractionation of boron isotopes. Several compounds never before studied as donors in the isotope exchange reaction were examined to determine if they were viable replacements for the dimethyl ether system. For the first time, ketones were studied as donors in the boron isotope exchange reaction. The ketones examined were acetone, methyl isobutyl ketone, and diisobutyl ketone. The ideal single stage separation factors, α, measured for these ketones were between 1.038 and 1.043 at 30°C. The observed separation factor for a fourth donor system, nitromethane, was 1.067 at 30°C, well above that predicted by theory or observed for any known BF3/donor system. For each of the systems studied, the separation factors were greater than the value of α = 1.027 reported for the dimethyl ether/BF3 system at 30°C. In view of the experimentally observed separation factors, these donor systems are potential replacements for dimethyl ether in large-scale boron isotope fractionation schemes. It is concluded that plant size could be significantly reduced by using any of these donors rather than dimethyl ether.
CO2 Uptake of Carbonation-Cured Cement Blended with Ground Volcanic Ash (2018)

Accelerated carbonation curing (ACC) as well as partial replacement of cement with natural minerals are examples of many previous approaches, which aimed to produce cementitious products with better properties and environmental amicabilities. In this regard, the present study investigates CO₂ uptake of carbonation-cured cement blended with ground Saudi Arabian volcanic ash (VA). Paste samples with cement replacement of 20%, 30%, 40%, and 50% by mass were prepared and carbonation-cured after initial curing of 24 h. A compressive strength test, X-ray diffractometry (XRD), and thermogravimetry were performed. Although pozzolanic reaction of VA hardly occurred, unlike other pozzolana in blended cement, the results revealed that incorporation of VA as a supplementary cementitious material significantly enhanced the compressive strength and diffusion of CO₂ in the matrix. This increased the CO₂ uptake capacity of cement, reducing the net CO₂ emission upon carbonation curing.
A Gyroscopic Damper System – A Damping Control with New Performance (2015)

A new approach substitutes the damper of a passenger car by a cardanic gimbaled flywheel mass. The constructive design leads to a rotary damper in which the vertical movement of the wheel carrier leads to revolution of the rotational axis of the flywheel. In this arrangement, the occurring precession moments are used to control damping moments and to store vibration energy. Different damper characteristics are achieved by different velocities of the inner ring. From almost zero torque output to high torque output, this damper has a huge spread. In this paper a control concept for a LQR is presented. The objective of the control is an adequate motion of the gyroscope to a desired damping characteristic of a passenger car.
C-Type Natriuretic Peptide in Complicated Pregnancy: Increased Secretion Precedes Adverse Events (2014)

Maternal NTproCNP is significantly raised in women who later exhibit a range of obstetric adverse events. Lack of association with placental concentrations suggests that these changes represent an adaptive response within the maternal circulation to a threatened nutrient supply to the fetus.
<sup>13</sup>C isotopomer analyses in intact tissue using {<sup>13</sup>C}homonuclear decoupling (1994)

Entry of 13C-enriched acetyl-CoA into the citric acid cycle results in scrambling of 13C into the various carbon positions of all intermediate pools. The eventual result is that the 13C resonances of all detectable intermediates or molecules exchanging with those intermediates appear as multiplets due to nearest neighbor spin-spin couplings. We have previously shown that an isotopomer analysis of the glutamate 13C multiplets provides a history of 13C flow through the cycle pools and that relative substrate utilization and relative anaplerotic flux can be quantitated (C.R. Malloy, A.D. Sherry, and F.M.H. Jeffrey, Am. J. Physiol. 259, H987-H995 (1990)). A major limitation of the method for in vivo applications is spectral resolution of multiline resonances required for a complete isotopomer analysis. We now show that [13C]homonuclear decoupling of the glutamate C3 resonance collapses nine-line C4 and C2 resonances into three-line multiplets. We demonstrate that these three-line 13C multiplets are well resolved in isolated, perfused rat hearts and present steady-state equations that allow an isotopomer analysis from data obtained in intact tissue. This advancement offers for the first time the possibility of extending 13C isotopomer methods to complex metabolic conditions in vivo.
Genetic treatment of severe hemoglobinopathies: the combat against transgene variegation and transgene silencing. (1998)

Gene addition strategies are rational approaches to the treatment of sickle cell anemia and thalassemia. The goal of such genetic treatments is to introduce a functional globin transcription unit in hematopoietic stem cells and express the transgene in a manner that is erythroid-specific, elevated, relatively constant from one cell to another, and sustained over time. Gene transfer is mediated by an expanding array of viral and nonviral vectors. High-titer retroviral vectors harboring the human beta-globin gene and the core sequences of the human beta-globin locus control region yield erythroid-specific gene expression in erythroid cell lines and in short-term murine bone marrow chimeras. However, we show that expression remains subject to position effect variegation and often decreases over time in vivo. Rather than a progressive transcriptional silencing in all cells, we ascribe the waning expression to the gradual emergence in blood of erythroid progeny derived from more and more primitive precursor cells in the months after transplantation. In our model, transgene expression is therefore determined by the integration site and the differentiation stage of the transduced cell at the time of integration. Globin expression is thus different in the progeny of a transduced erythroid progenitor cell and in the erythroid progeny of a transduced hematopoietic stem cell, reflecting the effect of flanking chromatin in differentiated cells and of chromatin remodeling at the site of integration in the progeny of multipotential cells. This model predicts that insulators and matrix attachment regions could be highly valuable to gene therapy in combination with potent transcriptional activators. When efficient gene transfer in hematopoietic stem cells is achieved at last, the challenge will be to regulate gene expression in vivo and overcome transgene variegation and transgene silencing.
A Dual-Mode Wi-Fi/BLE Wake-Up Receiver (2021)

This article presents a dual-mode wake-up receiver (WuRX) compatible with both Bluetooth-Low-Energy (BLE) and Wi-Fi transmitters. The proposed WuRX achieves the state-of-the-art power (as low as 4.4 μW through a latency-power duty-cycled tradeoff), sensitivity (as low as -92 dBm), and interference resilience (signal-to-interference ratio (SIR) =-67 dB) via a carefully architected frequency plan that supports BLE advertisement channel hopping or a proposed subcarrier-based within-channel Wi-Fi frequency hopping scheme, a carefully crafted frequency down-conversion plan that enables low-power receiver architecture via integer- N arithmetic, and an on-chip image rejection filter for full on-chip integration. The proposed design is implemented in a 65-nm CMOS process and operates from a 0.5-V supply.
Recent progress in distributed optical fiber raman sensors (2012)

A brief review of recent progress in researches, productions and applications of distributed fiber Raman sensors at China Jiliang University (CJLU) is presented. In order to improve the measurement distance, the accuracy, the space resolution, the ability of multi-parameter measurements, and the intelligence of distributed fiber sensor systems, a new generation fiber sensor technology based on the optical fiber nonlinear scattering fusion principle is proposed. A series of new generation distributed fiber sensors are investigated and designed, which consist of new generation ultra-long distributed fiber Raman and Rayleigh scattering sensors integrated with a fiber Raman amplifier (FRA), auto-correction full distributed fiber Raman temperature sensors based on Raman correlation dual sources, distributed fiber Raman temperature sensors based on a pulse coding source, distributed fiber Raman temperature sensors using a fiber Raman wavelength shifter, a new type of Brillouin optical time domain analyzers (BOTDA) integrated with a fiber Raman amplifier, distributed fiber Raman and Brillouin sensors integrated with a fiber Raman amplifier, and distributed fiber Brillouin sensors integrated with a fiber Brillouin frequency shifter. Sensor networks are important components of the internet of things. The distributed optical fiber sensor network (Rayleigh, Raman, and Brillouin scattering) is a 3S (smart materials, smart structure, and smart skill) system, which is easy to construct smart fiber sensor networks. The distributed optical fiber sensor has been applied to the power grids, railways, bridges, tunnels, roads, constructions, water supply systems, dams, oil and gas pipelines and other facilities, and can be integrated with wireless networks.
Maximum Diversity Space-Time Systems with Maximum Rate for Any Number of Antennas (2002)

Various multi-antenna designs have been developed in recent years targeting either highperformance, or, high rate. In this paper, we design a layered space-time (ST) scheme equipped with linear complex field (LCF) coding, which enables full diversity with full rate, for any number of transmit- and receive-antennas. Our theoretical claims are confirmed by simulations.
Abstract 3397: ￼A novel clinical tool that provides quantitative and accurate measurement of Met protein (2015)

Abstract BACKGROUND: Overexpression of Met tyrosine kinase receptor is associated with poor prognosis. Overexpression, and particularly MET amplification, are predictive of response to Met-specific therapy in preclinical models. Immunohistochemistry (IHC) of formalin-fixed paraffin-embedded (FFPE) tissues is currently used to select for ‘high Met’ expressing tumors for Met inhibitor trials. IHC suffers from antibody non-specificity, lack of quantitative resolution, and, when quantifying multiple proteins, inefficient use of scarce tissue. METHODS: After describing the development of the Liquid-Tissue-Selected Reaction Monitoring-mass spectrometry (LT-SRM-MS) Met assay, we evaluated the expression level of Met in 130 FFPE gastroesophageal cancer (GEC) tissues. We assessed the correlation of SRM Met expression to IHC and mean MET gene copy number (GCN)/nucleus or MET/CEP7 ratio by fluorescence in situ hybridization (FISH). RESULTS: Proteomic mapping of recombinant Met identified 418TEFTTALQR426 as the optimal SRM peptide. Limits of detection (LOD) and quantitation (LOQ) for this peptide were 150 and 200 amol/μg tumor protein, respectively. The assay demonstrated excellent precision and temporal stability of measurements in serial sections analyzed one year apart. Expression levels of 130 GEC tissues ranged (&lt;150 amol/μg to 4669.5 amol/μg. High correlation was observed between SRM Met expression and both MET GCN and MET/CEP7 ratio as determined by FISH (n = 30; R2 = 0.898). IHC did not correlate well with SRM (n = 44; R2 = 0.537) nor FISH GCN (n = 31; R2 = 0.509). A Met SRM level of &gt;1500 amol/μg was 100% sensitive (95% CI 0.69-1) and 100% specific (95% CI 0.92-1) for MET amplification. CONCLUSIONS: The Met SRM assay measured the absolute Met levels in clinical tissues with high precision. Compared to IHC, SRM provided a quantitative and linear measurement of Met expression, reliably distinguishing between non-amplified and amplified MET tumors. These results demonstrate a novel clinical tool for efficient tumor expression profiling, potentially leading to better informed therapeutic decisions for patients with GEC. Citation Format: Fabiola Cecchi, Wei-Li Liao, Sheeno Thyparambil, Marlene Darfler, David Krizman, Todd Hembrough, Jon Burrows, Don Bottaro, Daniel V.T. Catenacci. ￼A novel clinical tool that provides quantitative and accurate measurement of Met protein. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 3397. doi:10.1158/1538-7445.AM2015-3397
Hierarchical Contrastive Motion Learning for Video Action Recognition (2020)

One central question for video action recognition is how to model motion. In this paper, we present hierarchical contrastive motion learning, a new self-supervised learning framework to extract effective motion representations from raw video frames. Our approach progressively learns a hierarchy of motion features that correspond to different abstraction levels in a network. This hierarchical design bridges the semantic gap between low-level motion cues and high-level recognition tasks, and promotes the fusion of appearance and motion information at multiple levels. At each level, an explicit motion self-supervision is provided via contrastive learning to enforce the motion features at the current level to predict the future ones at the previous level. Thus, the motion features at higher levels are trained to gradually capture semantic dynamics and evolve more discriminative for action recognition. Our motion learning module is lightweight and flexible to be embedded into various backbone networks. Extensive experiments on four benchmarks show that the proposed approach consistently achieves superior results.
Improved vertically stacked Si/SiGe resonant interband tunnel diode pair with small peak voltage shift and unequal peak currents (2004)

A vertically integrated and serially connected npnp Si-based resonant interband tunnelling diode (RITD) pair is realised with low temperature molecular beam epitaxy (MBE) by monolithically stacking two RITDs with different spacer thicknesses. The asymmetric design manifests as unequal peak current densities that provide for much larger and uniform separation of the holding states for multi-valued logic. A δ-doped backwards diode connects the two serially connected RITDs with a very small series resistance. The I–V characteristic of the improved vertically integrated RITDs demonstrates two negative differential resistance (NDR) regions in the forward biasing condition with a small peak shift and unequal peak currents.
High-Speed Defect Detection in Rails by Noncontact Guided Ultrasonic Testing (2005)

Recent train accidents have reaffirmed the need to develop rail defect detection systems that are more effective than those used today. This paper proposes new inspection systems for detecting transverse-type cracks in the rail head, notoriously the most dangerous flaws in rails. In principle these systems can be applied to both continuous welded rail and jointed tracks because bidirectional inspection can be implemented. However, the systems may fail to detect defects located close to a joint. The proposed technology uses ultrasonic guided waves that are detected by remote sensors positioned as far away as 76 mm (3 in.) from the top of the rail head. An impulse hammer is used to generate waves below 50 kHz that can successfully detect cracks larger than 15% of the head cross-sectional area. For smaller crack-those as shallow as 1 mm-a pulsed laser is used for generating waves above 100 kHz. The inspection ranges are at least 10 m (32 ft) for cracks larger than 15% of the head area and at least 500 mm (20...