gebhart/patrepeval huggingface dataset:
Effect of Polyhydroxybutyrate (PHB) storage on l-arginine production in recombinant Corynebacterium crenatum using coenzyme regulation (2016)

Accumulation of PHB by introducing PHB synthesis pathway, together with up-regulation of coenzyme level by overexpressing NAD kinase, enables the recombinant C. crenatum to serve as high-efficiency cell factories in the long-time L-arginine fermentation. Furthermore, batch cultivation of the engineered C. crenatum revealed that it could accumulate both extracellular L-arginine and intracellular PHB simultaneously. All of these have a potential biotechnological application as a strategy for high-yield L-arginine.
Rendering 3-D High Dynamic Range Images: Subjective Evaluation of Tone-Mapping Methods and Preferred 3-D Image Attributes (2012)

High dynamic range (HDR) images provide superior picture quality by allowing a larger range of brightness levels to be captured and reproduced than traditional 8-bit low dynamic range (LDR) images. Even with existing 8-bit displays, picture quality can be significantly improved if content is first captured in HDR format, and then is tone-mapped to convert it from HDR to the LDR format. Tone mapping methods have been extensively studied for 2-D images. This paper addresses the problem of presenting stereoscopic tone-mapped HDR images on 3-D LDR displays and how it is different from the 2-D scenario. We first present a subjective psychophysical experiment that evaluates existing tone-mapping operators on 3-D HDR images. The results show that 3-D content derived using tone-mapping is much preferred to that captured directly with a pair of LDR cameras. Global (spatially invariant) and local (spatially variant) tone-mapping methods have similar 3-D effects. The second part of our study focuses on how the preferred level of brightness and the preferred amount of details differ between 3-D and 2-D images by conducting another set of subjective experiments. Our results show that while people selected slightly brighter images in 3-D viewing compared to 2-D, the difference is not statistically significant. However, compared to 2-D images, the subjects consistently preferred having a greater amount of details when watching 3-D. These results suggest that 3-D content should be prepared differently (sharper and possibly slightly brighter) from the same content intended for 2-D displaying, to achieve optimal appearance in each format. The complete database of the original HDR image pairs and their LDR counterparts are available online.
Directional gamma-ray scintillation detectors for searching radioisotopes (2003)

This paper describes a directional gamma-ray detector with different types of scintillators. The detector, which positively increases directional sensitivity to incident gamma rays, has been developed to measure energy and a direction of gamma rays, and to search radiation sources such as radioisotopes. Experimental results have shown that this detector has a potential for practical use in real fields.
Development of a New DNA Vaccine for Alzheimer Disease Targeting a Wide Range of Aβ Species and Amyloidogenic Peptides (2013)

It has recently been determined that not only Aβ oligomers, but also other Aβ species and amyloidogenic peptides are neurotoxic in Alzheimer disease (AD) and play a pivotal role in AD pathogenesis. In the present study, we attempted to develop new DNA vaccines targeting a wide range of Aβ species. For this purpose, we first performed in vitro assays with newly developed vaccines to evaluate Aβ production and Aβ secretion abilities and then chose an IgL-Aβx4-Fc-IL-4 vaccine (designated YM3711) for further studies. YM3711 was vaccinated to mice, rabbits and monkeys to evaluate anti-Aβ species antibody-producing ability and Aβ reduction effects. It was found that YM3711 vaccination induced significantly higher levels of antibodies not only to Aβ1-42 but also to AD-related molecules including AβpE3-42, Aβ oligomers and Aβ fibrils. Importantly, YM3711 significantly reduced these Aβ species in the brain of model mice. Binding and competition assays using translated YM3711 protein products clearly demonstrated that a large part of antibodies induced by YM3711 vaccination are directed at conformational epitopes of the Aβ complex and oligomers. Taken together, we demonstrate that YM3711 is a powerful DNA vaccine targeting a wide range of AD-related molecules and is worth examining in preclinical and clinical trials.
A new technique for joint optimization of excitation and model parameters in parametric speech coders (2001)

In a speech coding system, synthesis error (the difference between the original speech at the encoder input and the reproduced speech at the decoder output) is a more relevant measure of signal distortion than linear prediction (LP) error. By minimizing the synthesis error instead of the linear prediction error, the analysis and synthesis stages become more compatible. While LP error is linear in filter parameters, synthesis error is a highly nonlinear function of these parameters, making it computationally intractable for real-time applications. This paper presents a computationally feasible solution for minimizing the synthesis error applicable for joint optimization of the excitation and model parameters in real time. Using a gradient search in the root domain, synthesis error is minimized by re-optimizing the filter parameters for a given excitation. Starting the gradient search from the LPC solution, the resultant synthesis error produced by the proposed technique is guaranteed to be lower than the synthesis error using the LPC filter. By adding an extra minimization step, this technique can be incorporated into any parametric speech coder including LPC, multipulse LPC and CELP speech coders. The new technique and results of application to real speech will be presented.
Optical demodulation at 12 THz with applications to frequency metrology (1996)

Quantum interference in excitation of the 6S/sub 1/2/ 6P/sub 3/2/ 6D/sub 5/2/ transition in atomic cesium has been exploited for frequency demodulation of optical fields over an interval of +12 THz. In our scheme, the atom is employed as a nonlinear mixing element to compare the sum frequency for stepwise excitation with the doubled frequency for direct two photon excitation of an atomic ladder.
Non-linear 12-lead ECG synthesis from two intracardiac recordings (2009)

International audience
Simulation and measurement of the optical excitation of the S1 zero group velocity Lamb wave resonance in plates (2007)

Recent reports on the thermoelastic generation of Lamb waves in isotropic elastic plates show that a laser source efficiently excites a resonance that occurs at the minimum frequency of the first order symmetric (S1) Lamb mode. The group velocity of the Lamb wave goes to zero at this frequency while the phase velocity remains finite, and the resonance is referred to as the S1 zero group velocity (S1 ZGV) resonance. The S1 ZGV resonance can be employed for the nondestructive evaluation of the elastic properties of plates or plate thickness. A model for the generation of elastic waves in plates using an intensity-modulated continuous wave laser source is developed and used to study the behavior of the S1 ZGV resonance. The effects of the laser source parameters on the generation of the S1 ZGV resonance are explored, and the spatial distribution of the displacement produced at the resonance frequency is determined. The predicted displacement spectrum of Lamb waves generated in micron scale plates is found to compare well with experimental measurements. In addition, experimental measurements demonstrate that the S1 ZGV resonance can be used to map subsurface features in thin (4μm) membranes at high ultrasonic frequencies (700MHz).
Possible mechanisms of switching in two-ports based on 2D photonic crystals with magneto-optical resonators (2013)

We analyze possible mechanisms of switching in two-ports based on 2D photonic crystals (PhCs) with a magneto-optical resonator. The input and output waveguides can be side or front coupled with the resonator. The resonator operates with a dipole mode. In the switch with front coupling in nonmagnetic state the standing dipole mode provides equal nonzero wave amplitudes in the input and output waveguides and therefore transmission of the signal from the input to output waveguides. This is the state on. The applied magnetic field normal to the plane of the photonic crystal rotates the standing dipole mode by 90° setting the nodes in the input and output waveguides. This corresponds to the state off. On the contrary, in the switch with side coupling and nonmagnetized resonator, the standing dipole mode excited by a wave in the input waveguide has its node in the output waveguide. Therefore, the signal is reflected from the input port. This correspond to the state off of the switch. Magnetization by a DC magnetic field produces a rotating dipole pattern in the cavity. Due to this rotating mode signal passes from the input port to output one and this is the state on.
Cellular immunity to a determinant common to glutamate decarboxylase and coxsackie virus in insulin-dependent diabetes. (1994)

Insulin-dependent diabetes (IDD) results from the autoimmune destruction of the insulin-producing pancreatic beta cells. Autoreactive T-lymphocytes are thought to play a pivotal role in the pathogenesis of IDD; however, the target antigens of these cells, as well as the inductive events in the disease, are unclear. PBMC in persons with or at increased risk for IDD show elevated reactivity to the beta cell enzyme glutamate decarboxylase (GAD). To identify the T-lymphocyte-reactive determinants of GAD, an overlapping set of synthetic peptides was used to stimulate the PBMC from these individuals, PBMC responsiveness to GAD peptides was not restricted to those with IDD, and a number of peptides elicited responses in PBMC. However, the major determinant of GAD recognized by persons at increased risk for IDD was amino acids 247-279, a region which has significant sequence similarity to the P2-C protein of Coxsackie B virus (47% of 15 increased risk [islet cell autoantibody-positive relatives]; 25% of 16 newly diagnosed IDD patients; and 0% of 13 healthy control subjects). Responses to tetanus and insulin antigens were not different between the study groups. In addition, PBMC from individuals responding to GAD peptides within 247-279 also responded to a Coxsackie viral peptide (i.e., P2-C amino acids 32-47), an observation supporting potential molecular mimicry in this immune response. Although the role of environmental agents in the pathogenesis of the disease remains unclear, these cellular immunological findings support the epidemiological evidence suggesting an inductive role for enteroviruses like Coxsackie B in the autoimmunity underlying IDD.
Standardization and Quantitative Imaging With Photon-Counting Detector CT (2023)

Computed tomography (CT) images display anatomic structures across 3 dimensions and are highly quantitative; they are the reference standard for 3-dimensional geometric measurements and are used for 3-dimensional printing of anatomic models and custom implants, as well as for radiation therapy treatment planning. The pixel intensity in CT images represents the linear x-ray attenuation coefficient of the imaged materials after linearly scaling the coefficients into a quantity known as CT numbers that is conveyed in Hounsfield units. When measured with the same scanner model, acquisition, and reconstruction parameters, the mean CT number of a material is highly reproducible, and quantitative applications of CT scanning that rely on the measured CT number, such as for assessing bone mineral density or coronary artery calcification, are well established. However, the strong dependence of CT numbers on x-ray beam spectra limits quantitative applications and standardization from achieving robust widespread success. This article reviews several quantitative applications of CT and the challenges they face, and describes the benefits brought by photon-counting detector (PCD) CT technology. The discussed benefits of PCD-CT include that it is inherently multienergy, expands material decomposition capabilities, and improves spatial resolution and geometric quantification. Further, the utility of virtual monoenergetic images to standardize CT numbers is discussed, as virtual monoenergetic images can be the default image type in PCD-CT due to the full-time spectral nature of the technology.
Design for a Fourier-transform holographic microscope incorporating digital image reconstruction (1988)

A design for a Fourier-transform x-ray holographic microscope to operate in and above the water window is presented. The use of an x-ray laser light source for microscopic imaging offers several advantages. Primarily, imaging of live hydrated biological specimens would be possible with resolution in three dimensions. The beam illuminates both a spherical reference scatterer and the specimen, which can be simultaneously dropped into the path of the beam or statically mounted on a very thin foil. Charge-coupled devices are used for recording, their output being digitized and linked directly to a computer. It is shown that such a configuration is optimum, given the expected properties of a practical x-ray laser source.
Anchored periplasmic expression, a versatile technology for the isolation of high-affinity antibodies from <i>Escherichia coli</i> -expressed libraries (2004)

Anchored periplasmic expression (APEx) is a technology for the isolation of ligand-binding proteins from combinatorial libraries anchored on the periplasmic face of the inner membrane of Escherichia coli. After disruption of the outer membrane by Tris-EDTA-lysozyme, the inner-membrane-anchored proteins readily bind fluorescently labeled ligands as large as 240 kDa. Fluorescently labeled cells are isolated by flow cytometry, and the DNA of isolated clones is rescued by PCR. By using two rounds of APEx, the affinity of a neutralizing antibody to the Bacillus anthracis protective antigen was improved >200-fold, exhibiting a final K(D) of 21 pM. This approach has several technical advantages compared with previous library screening technologies, including the unique ability to screen for ligand-binding proteins that bind endogenously expressed ligands fused to a short-lived GFP. Further, APEx is able to display proteins either as an N-terminal fusion to a six-residue sequence derived from the native E. coli lipoprotein NlpA, or as a C-terminal fusion to the phage gene three minor coat protein of M13. The latter fusions allow hybrid phage display/APEx strategies without the need for further subcloning.
Patterned Optoelectronic Tweezers: A New Scheme for Selecting, Moving, and Storing Dielectric Particles and Cells (2018)

Optical micromanipulation has become popular for a wide range of applications. In this work, a new type of optical micromanipulation platform, patterned optoelectronic tweezers (p-OET), is introduced. In p-OET devices, the photoconductive layer (that is continuous in a conventional OET device) is patterned, forming regions in which the electrode layer is locally exposed. It is demonstrated that micropatterns in the photoconductive layer are useful for repelling unwanted particles/cells, and also for keeping selected particles/cells in place after turning off the light source, minimizing light-induced heating. To clarify the physical mechanism behind these effects, systematic simulations are carried out, which indicate the existence of strong nonuniform electric fields at the boundary of micropatterns. The simulations are consistent with experimental observations, which are explored for a wide variety of geometries and conditions. It is proposed that the new technique may be useful for myriad applications in the rapidly growing area of optical micromanipulation.
Abstract 38: A Decoy Peptide Selectively Inhibiting Dephosphorylation of Phospholamban (2012)

Cardiac sarcoplasmic reticulum Ca2+ ATPase (SERCA2a) plays a crucial role in Ca2+ handling in cardiomyocytes. Phospholamban (PLB) is an endogenous inhibitor of SERCA2a and its inhibitory activity is enhanced by dephosphorylation by protein phosphatase 1 (PP1). Therefore, blocking PP1-mediated dephosphorylation of PLB would be an efficient strategy for restoration of the reduced SERCA2a activity in failing hearts. We sought to develop a decoy peptide that mimics the phosphorylated PLB and thus competitively inhibits the PP1-mediated dephosphorylation of PLB. The phosphorylation sites, Ser16 and Thr17, are located within the flexible extra-membrane loop (amino acids 14-22) of PLB. We therefore synthesized a 9-mer pseudo-phosphorylated peptide derived from this region with a replacement of Ser16 with Glu (ψ-PLB-SE). Two other 9-mer peptides with wild type PLB sequence (ψ-PLB) or with a replacement of Ser16 with Ala (ψ-PLB-SA) were also synthesized. These peptides were coupled to a cell-permeable peptide TAT to facilitate cellular uptake. Treatment of adult rat cardiomyocytes with TAT-ψ-PLB-SE, but not with TAT-ψ-PLB or TAT-ψ-PLB-SA, significantly elevated the phosphorylation level of PLB, concomitant with an increase in contractile parameters in vitro. Perfusion of isolated rat hearts with TAT-ψ-PLB-SE significantly restored the left ventricular developed pressure that was suppressed by ischemia-reperfusion (Fig. 1). These data indicate that ψ-PLB-SE prevented dephosphorylation of PLB by acting as a decoy for PP1 and it would provide effective modality to regulate SERCA2a activity in failing hearts.
Cherry-MP: Correctly Integrating Checkpointed Early Resource Recycling in Chip Multiprocessors (2006)

Checkpointed early resource recycling (Cherry) is a recently-proposed microarchitectural technique that aims at improving critical resource utilization by performing aggressive resource recycling decoupled from instruction retirement, using a checkpoint/rollback mechanism to recover from occasional incorrect execution. In this paper, we explore correctness and performance issues that arise when Cherry-enabled processors are used in chip multiprocessor architectures. We propose mechanisms to address cache coherence, memory consistency, and forward progress issues in such environments. We also provide quantitative insight on the performance impact of the Cherry mechanism on parallel processing.
Point diffraction interferometry based on the use of two pinholes (2015)

Point diffraction interferometer (PDI) has become the high degree of accuracy device. In the optical wavefront testing the measurement accuracy is much higher than 1.0 nm RMS. In the paper there is presented a new version of PDI with two independently controlled beams using a pinhole plate with two pinholes as a beam coupler instead of a single-mode fiber or single-pinhole plate. Theoretical analysis of the pinhole diffraction wavefront and double pinholes diffraction interference is given. The PDI is used to investigate an interferometer reference lens and compare measurement results. The device can test high NA, the interference is obtained in circularly polarized light, and fringe contrast is adjustable to measure surfaces with different reflectance. The measurement repeatability now has been sub-nm RMS (measured NA = 0.33). The experiment result provides guarantee for the measurement in the high degree of accuracy. In the double pinholes PDI, generating two ideal spherical waves through two pinholes, one wave is as the reference wavefront for interference test, another ideal wavefront is reflected to the pinhole plate by the test mirror, and the tested wavefront and reference wavefront bring interference. Advantages of such arrangement of the PDI are: high maximum numerical aperture (NA = 0.55), distinct fringe patterns of high contrast, high accuracy of surface figure testing and wave-front repeatability RMS error 0.3 nm.
A respiratory gas exchange simulator for routine calibration in metabolic studies (1990)

We have developed a method for simulating respiratory gas exchange for on-line calibration of metabolic measurement systems. It utilizes a pump which intakes a mixture of atmospheric air and a known flow of precision-analysed calibration gas (21% CO2, 79% N2). It expels the resulting mixture with flow wave form and profiles of gas concentration which closely resemble those of normal expiration. Control of the calibration mixture's inflow allows the investigator to set any desired metabolic rate regardless of the minute ventilatory rate. This separation of metabolic from ventilatory rates provides a stringent test of the computational performance of the respiratory gas exchange measurement systems. The apparatus can reproduce any range of respiratory and metabolic performance (currently ranging from 0.2-5 l.min-1 O2 uptake and CO2 output) with accuracy +/- 2%.
Data-Centric Resource Management in Edge-Cloud Systems for the IoT (2019)

A major challenge in emergent scenarios such as the Cloud-assisted Internet of Things is efficiently managing the resources involved in the system while meeting requirements of applications. From the acquisition of physical data to its transformation into valuable services or information, several steps must be performed, involving the various players in such a complex ecosystem. Support for decentralized data processing on IoT devices and other devices near the edge of the network, in combination with the benefits of cloud technologies has been identified as a promising approach to reduce communication overhead, thus reducing delay for time sensitive IoT applications. The interplay of IoT, edge and cloud to achieve the final goal of producing useful information and value-added services to end user gives rise to a management problem that needs to be wisely tackled. The goal of this work is to propose a novel resource management framework for edge-cloud systems that supports heterogeneity of both devices and application requirements. The framework aims to promote the efficient usage of the system resources while leveraging the Edge Computing features, to meet the low latency requirements of emergent IoT applications. The proposed framework encompasses (i) a lightweight and data-centric virtualization model for edge devices, (ii) a set of components responsible for the resource management and the provisioning of services from the virtualized edge-cloud resources.
Characterization of Fossil Oil Fly Ash and the Enrichment of the Contained Vanadium as V4C3 by High-Temperature Treatment (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCharacterization of Fossil Oil Fly Ash and the Enrichment of the Contained Vanadium as V4C3 by High-Temperature TreatmentC. Aurisicchio, G. Bardi, A. Colligiani, F. Corigliano, L. D'Alessio, G. DeMaria, D. Ferro, G. Gazzoli, P. Primerano, and L. ScaramuzzaCite this: Chem. Mater. 1995, 7, 5, 865–870Publication Date (Print):May 1, 1995Publication History Published online1 May 2002Published inissue 1 May 1995https://pubs.acs.org/doi/10.1021/cm00053a009https://doi.org/10.1021/cm00053a009research-articleACS PublicationsRequest reuse permissionsArticle Views101Altmetric-Citations3LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts