gebhart/patrepeval huggingface dataset:
Stripe stabilization in vertical Bloch line memory (1991)

Partially grooved, long, rectangular grooves on the garnet surface were used to stabilize minor loop stripes in a vertical Bloch line memory. These stripes reside beneath the stripe confinement groove. The test chip contained 10 or 20 minor loop grooves, 10 read/write (r/w) gate grooves, and a major line groove. The film thickness was 4.76 μm, and the groove was 0.5 μm deep. Each minor loop groove was 500 μm long, four widths were tried: 3.5, 4, 4.5, and 5 μm. Two groove periods were tried: 10 and 20 μm. For stripe initialization, a bubble was nucleated in every minor loop groove by a single current pulse at a 90 Oe bias field. As the bias field was decreased slowly, bubbles began to stripe out at 80 Oe. At 75 Oe, all 10 stripes were stretched to the full groove length. These stripes were stable at bias fields between 70 and 80 Oe. Stripes decreased in length at higher fields, and escaped from the confinement groove at low fields. When stripes escaped from the groove, it always occured from the end not facing a r/w gate groove. These bias field values and the stripe-escape asymmetry are confirmed by computer simulation.
Efficient Constitutive Production of Human Fibroblast Interferon by Hamster Cells Transformed with the IFN-β <sub>1</sub> Gene Fused to An SV40 Early Promoter (1984)

The coding sequence of the human interferon (IFN)-beta 1 gene, fused 60 bp downstream from the RNA start site of the SV40 early gene, was transfected into dihydrofolate reductase (DHFR)-deficient Chinese hamster ovary (CHO) cells together with a selectable DHFR gene. Most transformants continuously secreted IFN-beta 1 into the medium. Induction did not stimulate expression of the fused SV40-IFN-beta 1 gene. The role of the SV40 promoter was verified by transforming cells with the unmodified human IFN-beta 1 gene, or by the IFN-beta 1 coding region fused to another poly(rI):(rC)-inducible gene. In these cases, the transformants showed strictly inducible (not constitutive) IFN secretion. By selection for methotrexate resistance, CHO clones with a 10-20-fold amplification of the SV40-IFN-beta 1 DNA were obtained. Such clones constitutively produce up to 350,000 units IFN/ml per 10(6) cells/24 hr, i.e., over 10 times more than fully induced human fibroblasts. In continuous culture with daily changes of medium, accumulation of IFN-beta 1 is constant at a rate of 300,000 molecules per cell/hr. Batches of up to 16 mg of IFN-beta 1 produced by the transformed CHO cells were purified to homogeneity by affinity chromatography on monoclonal antibodies. This IFN appears identical in size, activity, and immunospecificity to the native human IFN-beta 1 glycoprotein.
Robust image hashing (2002)

The proliferation of digital images creates problems for managing large image databases, indexing individual images, and protecting intellectual property. This paper introduces a novel image indexing technique that may be called an image hash function. The algorithm uses randomized signal processing strategies for a non-reversible compression of images into random binary strings, and is shown to be robust against image changes due to compression, geometric distortions, and other attacks. This algorithm brings to images a direct analog of message authentication codes (MACs) from cryptography, in which a main goal is to make hash values on a set of distinct inputs pairwise independent. This minimizes the probability that two hash values collide, even, when inputs are generated by an adversary.
Accurate Depth Map Estimation from Small Motions (2017)

With the growing use of digital lightweight cameras, generating 3D information has become an important challenge in computer vision. Despite several attempts presented in the literature to solve this challenge, it remains an open problem when it comes to the structural accuracy of the depth map and the required baseline (distance between the first and the last frames) to capture a sequence of images. In this paper, a novel approach is proposed to compute a high quality dense depth map together with a semi-dense/dense 3D structure from a sequence of images captured on a narrow baseline. Computing the depth information from small motions has been a challenge for decades because of the uncertain calculation of depth values when using a small baseline - up to 12mm. The proposed method can, in fact, perform on a much wider range of baselines from 8 mm up to 400 mm while respecting the structure of the reference frame. The evaluation has been done on more than 10 sets of recorded small motion clips and for the wider baseline, on 7 sets of stereo images from Middlebury benchmark. Preliminary results indicate that the proposed method has a better performance in terms of structural accuracy in comparison with the current state of the art methods. Also, the performance of the proposed method remains stable even when only a low number of frames are available for processing.
Polymorphism of human platelet membrane glycoprotein IIb associated with the Baka/Bakb alloantigen system (1990)

Abstract The human Baka/Bakb alloantigen system has been implicated in the pathogenesis of post-transfusion purpura and neonatal alloimmune thrombocytopenic purpura. Human alloantisera specific for either the Baka or Bakb allele have been shown to react exclusively with the heavy chain of membrane glycoprotein (GP) IIb. To investigate the structure of the Bak epitopes, we used the polymerase chain reaction (PCR) to amplify GPIIb cDNA synthesized from platelet RNA samples prepared from individuals of known serologic phenotype. Subsequent DNA sequence analysis of amplified GPIIb cDNAs derived from one Baka homozygous individual and one Bakb homozygous individual revealed a single nucleotide base difference near the 3′ end of the mRNA encoding the GPIIb heavy chain. Short 13 base allele-specific oligonucleotides (ASO) containing the putative phenotype-specific base in the middle were then synthesized, end-labeled with digoxigenin-11-dUTP using terminal transferase, and used as probes in subsequent dot-blot hybridization experiments. Platelet RNA was prepared from a panel made up of four Baka/a, three Bakb/b, and two Baka/b individuals, and the mRNA encoding GPIIb was amplified using PCR and spotted onto nylon membranes. ASO hybridization showed that the nucleotide base difference identified above segregated with Bak phenotype in all nine individuals examined (P = .002). The base pair substitution results in an amino acid polymorphism at residue 843 of the mature heavy chain. The Baka form of GPIIb encodes an isoleucine at this position, whereas the Bakb allele contains a serine. Identification of the polymorphism associated with this clinically important alloantigen system should permit new therapeutic and diagnostic approaches for treating and managing patients with alloimmune thrombocytopenic disorders.
Drones that see through waves – preliminary results from airborne fluid lensing for centimetre‐scale aquatic conservation (2016)

Abstract The use of fluid lensing technology on unmanned aerial vehicles (UAVs, or drones) is presented as a novel means for 3D imaging of aquatic ecosystems from above the water's surface at the centimetre scale. Preliminary results are presented from airborne fluid lensing campaigns conducted over the coral reefs of Ofu Island, American Samoa (2013) and the stromatolite reefs of Shark Bay, Western Australia (2014), covering a combined area of 15 km 2 . These reef ecosystems were revealed with centimetre‐scale 2D resolution, and an accompanying 3D bathymetry model was derived using fluid lensing, Structure from Motion and UAV position data. Data products were validated from in situ survey methods including underwater calibration targets, depth measurements and millimetre‐scale high‐dynamic‐range gigapixel photogrammetry. Fluid lensing is an experimental technology that uses water‐transmitting wavelengths to passively image underwater objects at high‐resolution by exploiting time‐varying optical lensing events caused by surface waves. Fluid lensing data are captured from low‐altitude, cost‐effective electric UAVs to achieve multispectral imagery and bathymetry models at the centimetre scale over regional areas. As a passive system, fluid lensing is presently limited by signal‐to‐noise ratio and water column inherent optical properties to ~10 m depth over visible wavelengths in clear waters. The datasets derived from fluid lensing present the first centimetre‐scale images of a reef acquired from above the ocean surface, without wave distortion. The 3D multispectral data distinguish coral, fish and invertebrates in American Samoa, and reveal previously undocumented, morphologically distinct, stromatolite structures in Shark Bay. These findings suggest fluid lensing and multirotor electric drones represent a promising advance in the remote sensing of aquatic environments at the centimetre scale, or ‘reef scale’ relevant to the conservation of reef ecosystems. Pending further development and validation of fluid lensing methods, these technologies present a solution for large‐scale 3D surveys of shallow aquatic habitats with centimetre‐scale spatial resolution and hourly temporal sampling. Copyright © 2016 John Wiley & Sons, Ltd.
A manganese superoxide dismutase from serratia marcescens. (1983)

Superoxide dismutase was easily isolated from Serratia marcescens ATCC 21074 by ammoniumsulfate fractionation and Dyematrex Gel Green A chromatography. The purified enzymewas a manganese-containing protein with a molecular weight of 4.8×104. Combination of gel electrophoresis in dodecylsulfate and gel filtration showed that it was composed of two equal molecular subunits. The dimeric protein contained 1.5 g atoms of manganese and exhibited molecular extinction coefficients of 9.4×104 M-1cm-1 at 280 nmand 8.9×102 M-1cm-1at 470 nm. The enzyme showed similarities with manganese superoxide dismutases from other prokaryotes.
Two-Dimensional Fractionation Method for Proteome-Wide Cross-Linking Mass Spectrometry Analysis (2022)

Cross-linking mass spectrometry (XL-MS) is an emergent technology for studying protein-protein interactions (PPIs) and elucidating architectures of protein complexes. The development of various MS-cleavable cross-linkers has facilitated the identification of cross-linked peptides, enabling XL-MS studies at the systems level. However, the scope and depth of cellular networks revealed by current XL-MS technologies remain limited. Due to the inherently broad dynamic range and complexity of proteomes, interference from highly abundant proteins impedes the identification of low-abundance cross-linked peptides in complex samples. Thus, peptide enrichment prior to MS analysis is necessary to enhance cross-link identification for proteome-wide studies. Although chromatographic techniques including size exclusion (SEC) and strong cation exchange (SCX) have been successful in isolating cross-linked peptides, new fractionation methods are still needed to further improve the depth of PPI mapping. Here, we present a two-dimensional (2D) separation strategy by integrating peptide SEC with tip-based high pH reverse-phase (HpHt) fractionation to expand the coverage of proteome-wide XL-MS analyses. Combined with the MS-cleavable cross-linker DSSO, we have successfully mapped <i>in vitro</i> PPIs from HEK293 cell lysates with improved identification of cross-linked peptides compared to existing approaches. The method developed here is effective and can be generalized for cross-linking studies of complex samples.
A Quantitative System Pharmacology Computer Model for Cognitive Deficits in Schizophrenia (2013)

Although the positive symptoms of schizophrenia are reasonably well-controlled by current antipsychotics, cognitive impairment remains largely unaddressed. The Matrics initiative lays out a regulatory path forward and a number of targets have been tested in the clinic, so far without much success. To address this translational disconnect, we have developed a mechanism-based humanized computer model of a relevant key cortical brain network with schizophrenia pathology involved with the maintenance aspect of working memory (WM). The model is calibrated using published clinical experiments on N-back WM tests. We further simulate the opposite effect of γ-aminobutyric acid (GABA) modulators lorazepam and flumazenil and of a published augmentation trial of clozapine with risperidone, illustrating the introduction of new targets and the capacity of predicting the effects of polypharmacy. This humanized approach allows for early prospective and quantitative assessment of cognitive outcome in a central nervous system (CNS) research and development project, thereby hopefully increasing the success rate of clinical trials.CPT: Pharmacometrics & Systems Pharmacology (2013) 2, e36; doi:10.1038/psp.2013.12; advance online publication 3 April 2013.
Schmallenberg Virus Recurrence, Germany, 2014 (2015)

Schmallenberg virus (SBV) emerged in Germany in 2011, spread rapidly across Europe, and almost disappeared in 2013. However, since late summer 2014, new cases have occurred in adult cattle. Full-genome analysis revealed some amino acid substitution differences from the first SBV sample. Viremia developed in experimentally infected sheep and cattle for 4-6 days.
Low-Power Network-Packet-Processing Architecture Using Process-Learning Cache for High-End Backbone Router (2005)

A novel cache-based packet-processing-engine (PPE) architecture that achieves low-power consumption and high packet-processing throughput by exploiting the nature of network traffic is proposed. This architecture consists of a processing-unit array and a bit-stream manipulation path called a burst stream path (BSP) that has a special cache mechanism called a process-learning cache (PLC). Network packets, which have the same information in their header, appear repeatedly over a short time. By exploiting that nature, the PLC memorizes the packet-processing method with all results (i.e., table lookups), and applies it to other packets. The PLC enables most packets to skip the execution at the processing-unit array, which consumes high power. As a practical implementation of the cache-based PPE architecture, P-Gear was designed. In particular, P-Gear was compared with a conventional PPE in terms of silicon die size and power consumption. According to this comparison, in the case of current 0.13-μm CMOS process technology, P-Gear can achieve 100-Gbps (gigabit per second) packet-processing throughput with only 36.5% of the die size and 32.8% of the power consumption required by the conventional PPE. Configurations of both architectures for the 1- to 100-Gbps throughput range were also analyzed. In the throughput range of 10-Gbps or more, P-Gear can achieve the target throughput in a smaller die size than the conventional PPE. And for the whole throughput range, P-Gear can achieve a target throughput at lower power than the conventional PPE.
A Dual-Rail Hybrid Analog/Digital Low-Dropout Regulator With Dynamic Current Steering for a Tunable High PSRR and High Efficiency (2020)

A dual-rail hybrid analog/digital low-dropout regulator (DRLDO) targeting heterogeneous integration in a multichip package (MCP) platform is presented. Different from the classic single-input-single-output low-dropout regulator (LDO) topology, which incurs an efficiency penalty due to a large dropout voltage, this DRLDO architecture breaks the tradeoff of power-supply rejection and high efficiency by exploiting two rails available in a typical MCP system on a chip. One rail is the larger dropout ac branch rail which helps with power-supply rejection while the other is the low-dropout dc branch that helps with maximizing efficiency. The hybrid combination of analog and digital branches achieves both high efficiency and high power-supply rejection ratio (PSRR) simultaneously. Moreover, a dynamic current steering mechanism actively regulates the current contribution between the two rails and flexibly tunes the PSRR and power conversion efficiency performances. Measurements on a 22-nm CMOS chip demonstrate up to -46-dB PSRR and 89% efficiency across a 0-80-mA load from 1.8-V HV and 1.05-V LV dual-input rails. It improves the efficiency of the conventional analog LDO (ALDO) in MCP applications up to 32% while still maintaining -20-dB PSRR performance.
HMG‐CoA Reductase Inhibitors from <i>Monascus</i>‐Fermented Rice (2013)

Seven compounds were isolated from Monascus ‐fermented rice by column chromatography with silica gel and semiprep HPLC. Their structures were elucidated by extensive spectroscopic methods. All compounds displayed HMG‐CoA reductase inhibitory potential, among them compound 7 exhibited strong inhibition with IC 50 value comparable with lovastatin. In this study, two compounds ( 1 and 2 ) were obtained from natural source for the first time.
Fetal Heart Rate Monitoring Implemented by Dynamic Adaptation of Transmission Power of a Flexible Ultrasound Transducer Array (2019)

Fetal heart rate (fHR) monitoring using Doppler Ultrasound (US) is a standard method to assess fetal health before and during labor. Typically, an US transducer is positioned on the maternal abdomen and directed towards the fetal heart. Due to fetal movement or displacement of the transducer, the relative fetal heart location (fHL) with respect to the US transducer can change, leading to frequent periods of signal loss. Consequently, frequent repositioning of the US transducer is required, which is a cumbersome task affecting clinical workflow. In this research, a new flexible US transducer array is proposed which allows for measuring the fHR independently of the fHL. In addition, a method for dynamic adaptation of the transmission power of this array is introduced with the aim of reducing the total acoustic dose transmitted to the fetus and the associated power consumption, which is an important requirement for application in an ambulatory setting. The method is evaluated using an in-vitro setup of a beating chicken heart. We demonstrate that the signal quality of the Doppler signal acquired with the proposed method is comparable to that of a standard, clinical US transducer. At the same time, our transducer array is able to measure the fHR for varying fHL while only using 50% of the total transmission power of standard, clinical US transducers.
Comparison of regenerative braking controllers for permanent magnet synchronous machines (2015)

Since most electric machine applications involve accelerating and decelerating mechanical loads, there is a potential to recover stored kinetic energy during braking events. However, this potential cannot be fully realized without proper design of the braking controller, since electric machine braking may be either regenerative or non-regenerative. This paper develops a novel method for designing regenerative braking controllers for permanent-magnet synchronous machines, based on the concept of using speed feedback to apply a fraction of the electromagnetically induced voltage at the machine terminals.
Direct and quantitative broadband absorptance spectroscopy on small objects using Fourier transform infrared spectrometer and bilayer cantilever probes (2013)

A measurement platform is introduced that combines a bilayer cantilever probe with a Fourier transform infrared spectrometer to measure absolute spectral absorptance between wavelengths of 3 μm and 18 μm directly and quantitatively. The enhanced sensitivity provided by the cantilever probe enables the quantitative characterization of micro- and nanometer-sized samples. Validation of the technique is carried out by measuring the absorptance spectrum of a doped silicon thin film with a backside aluminum layer and found to agree well with the theoretical predictions. The presented technique is especially attractive for samples such as individual nanowires or nanoparticles, isolated molecules, powders, and photonic structures.
Fabrication of robust, high-quality two-dimensional colloidal crystals from aqueous suspensions containing water-soluble polymer (2006)

In this letter, we report that two-dimensional (2D) polystyrene colloidal crystals of much higher quality can be fabricated in a single-step confined convective assembly process by introducing water-soluble polymer into the colloidal suspension. The presence of the water-soluble polymer in the aqueous phase enables the colloidal particles to arrange into more hexagonally close-packed single crystalline domains, and thus reduces the defect density. In addition, the physical stability of the final 2D colloidal crystals is improved because each particle is bonded to neighboring particles and to the substrate by polymer bridges.
Oncogenic potential of erbB-2 in human mammary epithelial cells. (1991)

Introduction of the normal erbB-2 gene into immortalized human mammary epithelial cells (184B5) by transfection conferred a growth advantage to these cells both in vitro and in vivo. The 184B5 cells overexpressing erbB-2 formed colonies in semi-solid medium, frequently induced transient nodules in athymic mice and produced progressive tumors in vivo at a low frequency. Those tumors which did arise from erbB-2-transfected cells displayed substantially higher levels of normal gp185erb-2 protein when compared to the original transfectants, consistent with their selection for increased erbB-2 expression. Introduction of genes encoding genetically altered erbB-2 molecules into 184B5 cells increased their colony-forming efficiency and converted the cells to a tumorigenic phenotype at a high frequency. When the biological and biochemical properties of human mammary carcinoma cell lines known to overexpress erbB-2 were compared to the transfected 184B5 lines, they behaved most like those overexpressing the normal erbB-2 protein. Results indicate that overexpression of normal erbB-2 may directly contribute to the transformation of human mammary epithelium if sufficient levels of erbB-2 protein are expressed or if the erbB-2 gene is genetically altered.
Identification of Arabidopsis<i>rat</i>Mutants (2003)

Limited knowledge currently exists regarding the roles of plant genes and proteins in the Agrobacterium tumefaciens-mediated transformation process. To understand the host contribution to transformation, we carried out root-based transformation assays to identify Arabidopsis mutants that are resistant to Agrobacterium transformation (rat mutants). To date, we have identified 126 rat mutants by screening libraries of T-DNA insertion mutants and by using various "reverse genetic" approaches. These mutants disrupt expression of genes of numerous categories, including chromatin structural and remodeling genes, and genes encoding proteins implicated in nuclear targeting, cell wall structure and metabolism, cytoskeleton structure and function, and signal transduction. Here, we present an update on the identification and characterization of these rat mutants.
Syntheses of Functionalized 1,4‐Disubstituted γ‐Carbolines. (2003)

Abstract For Abstract see ChemInform Abstract in Full Text.