gebhart/patrepeval huggingface dataset:
Comparative peptidomics of the endocrine pancreas: islet hormones from the clawed frog Xenopus laevis and the red-bellied newt Cynops pyrrhogaster (2002)

Electrospray mass spectrometry coupled with reverse-phase HPLC was used to identify peptides in the molecular mass range 3000-6000 Da in extracts of the pancreata of the clawed frog Xenopus laevis (Anura: Pipidae) and the red-bellied newt Cynops pyrrhogaster (Caudata: Salamandridae). Amino acid sequences of insulins, peptides derived from the post-translational processing of proglucagons and pancreatic polypeptide were determined by automated Edman degradation. Three molecular forms of insulin were isolated from the tetraploid organism X. laevis that represent insulin-1 and insulin-2, as deduced from the nucleotide sequences of previously characterized cDNAs, and a third form which differed from insulin-2 by the single amino acid substitution Asp(21)-->Glu in the B-chain. The amino acid sequence of Xenopus preproglucagons (genes 1 and 2 ) may be deduced from the nucleotide sequences of cDNAs but the pathways of post-translation processing of the precursors are not known. Two molecular forms of glucagon with 36 amino acids, derived from genes 1 and 2 and representing glucagon-29 extended from its C terminus by different heptapeptides, and five molecular forms of glucagon-like peptide 1 (GLP-1) were isolated. The GLPs represent proglucagon-(77-113), -(122-158) and -(160-191) from gene 1, and proglucagon-(77-113) and -(160-191) from gene 2. A single molecular form of insulin, glucagon-36, a C-terminally alpha-amidated GLP-1 with 30 amino acid residues, a 33 amino acid residue GLP-2 and pancreatic polypeptide were isolated from the pancreatic extract of the diploid organism C. pyrrhogaster. This study has illustrated the power of electrospray mass spectrometry for the rapid and reliable identification of peptides in chromatographic fractions without the need to use radioimmunoassay, radioreceptor assay or bioassay.
Conduction mechanism in highly doped β-Ga<sub>2</sub>O<sub>3</sub> single crystals grown by edge-defined film-fed growth method and their Schottky barrier diodes (2016)

Abstract Edge-defined fed-grown β-Ga 2 O 3 single crystals with high electron concentration of 3.9 × 10 18 cm −3 at 300 K were characterized by Hall effect measurement, and Schottky barrier diodes have been demonstrated. Electron mobility was as high as 74 cm 2 /(V·s) at 300 K regardless of the high doping concentration. The electron concentration did not change substantially in the low temperature below 160 K. This properties can be explained by the two-band model due to the inter-band conduction. On the Schottky barrier diodes, the rectification characteristics were clearly observed, and the current density of 96.8 A/cm 2 at the forward voltage of 1.6 V was obtained.
Evaluating Visual Information Provided by Audio Description (1996)

Short segments of two TV programs without audio description (AD) were presented to 25 subjects with low vision and 24 subjects with normal vision, and 29 additional subjects heard only the standard audio portions. The subjects then answered questions based on the visual information contained in the AD of the programs. The subjects with normal vision performed the best, followed by those with low vision and those who heard only the audio portion; all performed at better than chance levels. The results indicate that although AD may provide information on visual details to visually impaired audiences, some of the information in the AD may be obtained from the standard audio portion.
Cultural Familiarity, Foreign Language Speaking Skill, and Foreign Language Anxiety: The Case of Indonesian EFL Learners (2022)

Since cultural aspects of language learning are not incorporated in some EFL materials and textbooks, this study can be important to investigate the impacts of using cultural-based instruction on developing the speaking skill of Indonesian EFL learners. In addition, this research tried to examine the effects of using cultural-based instruction on foreign language anxiety of Indonesian EFL learners. To reach these aims, the Oxford Quick Placement Test (OQPT) was given to 83 EFL learners, and 50 of them were selected for the target sample of the current investigation. The selected respondents were then randomly divided into two equal experimental and control groups. Afterward, the Foreign Language Classroom Anxiety Scale (FLCAS) questionnaire was given to both groups. After providing the stated questionnaire, both groups were pretested by a speaking test. After that, the experimental group was instructed by applying a cultural-based instruction, while the control group received a noncultural-based education. After the instruction ended, the FLCAS questionnaire and the posttest of speaking were given to the respondents to determine the effects of the cultural-based instruction on their speaking skills and foreign language anxiety. The collected data were analyzed by using independent samples and paired sample <a:math xmlns:a="http://www.w3.org/1998/Math/MathML" id="M1"> <a:mi>t</a:mi> </a:math> -tests. The results revealed that the experimental group had better performances than the control group on the speaking and anxiety posttests. Finally, based on the results, some conclusions and implications were suggested.
Cardiac rhythm during temporal lobe seizures (1992)

We studied the neuroanatomic correlates of ictal tachycardia in 27 seizures from five patients with unilateral temporal lobe epilepsy being evaluated with bilateral temporal lobe depth electrodes and orbitofrontal subdural electrodes. There were 11 complex partial seizures, three simple partial seizures, and 13 subclinical seizures. For all seizures, heart rate (HR) increased in a graded fashion as new cortical regions anywhere in the brain were recruited into the seizure. HR plateaued at the new level despite EEG frequency changes until the next region became involved. Increases in HR did not correlate with increased duration of seizures but rather with volume of brain involved. Restricted amygdaloid seizure activity was generally insufficient to alter HR. We conclude that the amygdala has a limited role in modulating HR during seizures, and ictal tachycardia depends principally on the volume of cerebral structures recruited into a seizure.
Influences of Non-fullerene Acceptor Fluorination on Three-Dimensional Morphology and Photovoltaic Properties of Organic Solar Cells (2019)

Fluorination of conjugated molecules has been established as an effective structural modification strategy to influence properties and has attracted extensive attention in organic solar cells (OSCs). Here, we have investigated optoelectronic and photovoltaic property changes of OSCs made of polymer donors with the non-fullerene acceptors (NFAs) ITIC and IEICO and their fluorinated counterparts IT-4F and IEICO-4F. Device studies show that fluorinated NFAs lead to reduced <i>V</i><sub>oc</sub> but increased <i>J</i><sub>sc</sub> and fill-factor (FF), and therefore, the ultimate influence to efficiency depends on the compensation of <i>V</i><sub>oc</sub> loss and gains of <i>J</i><sub>sc</sub> and FF. Fluorination lowers energy levels of NFAs, reduces their electronic band gaps, and red-shifts the absorption spectra. The impact of fluorination on the molecular order depends on the specific NFA, and the conversion of ITIC to IT-4F reduces the structural order, which can be reversed after blending with the donor PBDB-T. Contrastingly, IEICO-4F presents stronger π-π stacking after fluorination from IEICO, and this is further strengthened after blending with the donor PTB7-Th. The photovoltaic blends universally present a donor-rich surface region which can promote charge transport and collection toward the anode in inverted OSCs. The fluorination of NFAs, however, reduces the fraction of donors in this donor-rich region, consequently encouraging the intermixing of donor/acceptor for efficient charge generation.
Endocrinology: Ovarian reserve test with the gonadotrophin-releasing hormone agonist buserelin: correlation with in-vitro fertilization outcome (1996)

A gonadotrophin-releasing hormone agonist stimulation test determination of follicle stimulating hormone (FSH) concentrations before and 2 h after buserelin injection was carried out in 78 in-vitro fertilization cycles, and compared with basal FSH concentrations to predict ovarian response. Ovarian response was quantified by the ratio of peak oestradiol concentration divided by the total dose of human menopausal gonadotrophin (HMG) administered, the most reproducible parameter in 11 patients who underwent two treatment cycles. Stimulation outcome was highly related to the buserelin test, the best prognostic indicator being the sum of FSH concentrations. However, basal FSH concentration achieved similar correlations, even in those patients aged > 35 years. Sensitivity, specificity, positive and negative predictive values of basal FSH concentration and sum of FSH concentrations were similar. Low basal concentration and sum of FSH concentrations were both associated with a better ovarian response. Construction of receiver operator characteristic curves demonstrated that basal FSH concentration was more informative than the sum of FSH concentrations. Finally, the sum of FSH concentrations did not increase the prediction of ovarian response variability. We conclude that the buserelin test is strongly predictive of stimulation outcome, but is no more informative than the usual screening. We suggest that the performance of other stimulation tests should be clearly compared with that of basal FSH concentration.
A New Synthesis of Some Non-Steroidal Anti-Inflammatory Agents via Cyanophosphates<sup>1</sup>) (1984)

Abstract A simple and convenient procedure for the syntheses of some nonsteroidal anti-inflammatory agents, ibuprofen, rac-naproxen, clidanac and loxoprofen, is described.
Target-Responsive “Sweet” Hydrogel with Glucometer Readout for Portable and Quantitative Detection of Non-Glucose Targets (2013)

Portable devices with the advantages of rapid, on-site, user-friendly, and cost-effective assessment are widely applied in daily life. However, only a limited number of quantitative portable devices are commercially available, among which the personal glucose meter (PGM) is the most successful example and has been the most widely used. However, PGMs can detect only blood glucose as the unique target. Here we describe a novel design that combines a glucoamylase-trapped aptamer-cross-linked hydrogel with a PGM for portable and quantitative detection of non-glucose targets. Upon target introduction, the hydrogel collapses to release glucoamylase, which catalyzes the hydrolysis of amylose to produce a large amount of glucose for quantitative readout by the PGM. With the advantages of low cost, rapidity, portability, and ease of use, the method reported here has the potential to be used by the public for portable and quantitative detection of a wide range of non-glucose targets.
DNA cytometry and cytology by quantitative fluorescence image analysis in symptomatic bladder cancer patients (1987)

A semi-automated quantitative fluorescence image analysis (QFIA) technique was developed with the Leitz TAS-Plus to detect bladder cancer using hyperploidy in urinary cells. Absolute nuclear fluorescence intensity (ANFI) (emission at 540 nm with excitation at 436 nm) of individual acridine-orange-stained cells was quantitated using (1) QFIA and (2) simple filter microspectrofluorophotometry (SFM). Both methods employed an internal phosphor particle standard which, when once calibrated against the DNA content of normal cells, obviates the necessity of routinely calibrating against normal cells in each sample. Results of SFM and QFIA were compared with routine Papanicolaou (Pap) cytopathology, using histopathology as the diagnostic standard in 272 samples from 67 symptomatic patients. The sensitivities for detecting low-grade transitional-cell carcinoma were 86% for SFM, 76% for QFIA, and 33% for Pap cytology. QFIA and SFM were significantly more sensitive at detecting bladder cancer than was Pap (0.01 greater than p greater than 0.001). Comparison of sensitivity obtained with bladder washings and urine samples showed that noninvasively obtained urines can be used. ANFI also detected recurrent and precancerous bladder lesions and kidney, ureter, and prostate lesions. This approach may prove generally useful in quantifying biochemical and immunological probes and should be broadly applicable as a research tool for studying the relationship of biochemical markers in the pathogenesis of disease and as a test for cancer control.
Evolutionary dynamics of the severe acute respiratory syndrome coronavirus 2 genomes (2022)

The coronavirus disease 2019 (COVID-19) pandemic has caused immense losses in human lives and the global economy and posed significant challenges for global public health. As severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of COVID-19, has evolved, thousands of single nucleotide variants (SNVs) have been identified across the viral genome. The roles of individual SNVs in the zoonotic origin, evolution, and transmission of SARS-CoV-2 have become the focus of many studies. This review summarizes recent comparative genomic analyses of SARS-CoV-2 and related coronaviruses (SC2r-CoVs) found in non-human animals, including delineation of SARS-CoV-2 lineages based on characteristic SNVs. We also discuss the current understanding of receptor-binding domain (RBD) evolution and characteristic mutations in variants of concern (VOCs) of SARS-CoV-2, as well as possible co-evolution between RBD and its receptor, angiotensin-converting enzyme 2 (ACE2). We propose that the interplay between SARS-CoV-2 and host RNA editing mechanisms might have partially resulted in the bias in nucleotide changes during SARS-CoV-2 evolution. Finally, we outline some current challenges, including difficulty in deciphering the complicated relationship between viral pathogenicity and infectivity of different variants, and monitoring transmission of SARS-CoV-2 between humans and animals as the pandemic progresses.
Magnetization dynamics, Bennett clocking and associated energy dissipation in multiferroic logic (2011)

It has been recently shown that the magnetization of a multiferroic nanomagnet, consisting of a magnetostrictive layer elastically coupled to a piezoelectric layer, can be rotated by a large angle if a tiny voltage of few tens of mV is applied to the piezoelectric layer.The potential generates stress in the magnetostrictive layer and rotates its magnetization by ~ 90 0 to implement Bennett clocking in nanomagnetic logic chains.Because of the small voltage needed, this clocking method is far more energy-efficient than those that would employ spin transfer torque or magnetic fields to rotate the magnetization.In order to assess if such a clocking scheme can be also reasonably fast, we have studied the magnetization dynamics of a multiferroic logic chain with nearest neighbor dipole coupling using the Landau-Lifshitz-Gilbert (LLG) equation.We find that clock rates of ~ 2 GHz are feasible while still maintaining the exceptionally high energy-efficiency.For this clock rate, the energy dissipated per clock cycle per bit flip is ~52,000 kT at room temperature in the clocking circuit for properly designed nanomagnets.Had we used spin transfer torque to clock at the same rate, the
G-protein based ELISA as a potency test for rabies vaccines (2017)

The NIH test is currently used to assess the potency of rabies vaccine, a key criterion for vaccine release. This test is based on mice immunization followed by intracerebral viral challenge. As part of global efforts to reduce animal experimentation and in the framework of the development of Sanofi Pasteur next generation, highly-purified vaccine, produced without any material of human or animal origin, we developed an ELISA as an alternative to the NIH test. This ELISA is based on monoclonal antibodies recognizing specifically the native form of the viral G-protein, the major antigen that induces neutralizing antibody response to rabies virus. We show here that our ELISA is able to distinguish between potent and different types of sub-potent vaccine lots. Satisfactory agreement was observed between the ELISA and the NIH test in the determination of the vaccine titer and their capacity to discern conform from non-conform batches. Our ELISA meets the criteria for a stability-indicating assay and has been successfully used to develop the new generation of rabies vaccine candidates. After an EPAA international pre-collaborative study, this ELISA was selected as the assay of choice for the EDQM collaborative study aimed at replacing the rabies vaccine NIH in vivo potency test.
Multiuser MIMO Indoor Visible Light Communication System Using Spatial Multiplexing (2017)

Visible light communications is an energy efficient and cost-effective solution for indoor wireless access. In this paper, we propose a multiple input multiple output system using centralized or decentralized transmitted power allocation algorithms with multiple LEDs and photodetectors. The proposed system uses an optical code division multiple-access technique to support multiple users. Time-space minimum mean squared error filters at the receivers are designed to diminish the effect of multiple-access interference. In the centralized power allocation algorithm, all the LED lamps in the room are coordinated and controlled by a central controller; each LED lamp supports all the users within the indoor area. The decentralized power allocation algorithms we propose have similar bit error rate performance yet less computational burden compared to the centralized algorithm. In our decentralized algorithms, users are supported by a subset of the LEDs, and so the optimization problem size can be reduced by as much as 93%. For each receiver, multiple photodetectors with different orientations are employed to improve the signal to interference plus noise ratio. In addition, some practical considerations such as shadowing effects, illumination requirements, dimming control, and transmitted power quantization are taken into account.
Gigantic Montages with a Fully Automated FE-SEM (Serial Sections of a Mouse Brain Tissue) (2010)

Extended abstract of a paper presented at Microscopy and Microanalysis 2010 in Portland, Oregon, USA, August 1 – August 5, 2010.
Test-Time Fast Adaptation for Dynamic Scene Deblurring via Meta-Auxiliary Learning (2021)

In this paper, we tackle the problem of dynamic scene deblurring. Most existing deep end-to-end learning approaches adopt the same generic model for all unseen test images. These solutions are sub-optimal, as they fail to utilize the internal information within a specific image. On the other hand, a self-supervised approach, SelfDeblur, enables internal training within a test image from scratch, but it does not fully take advantage of large external datasets. In this work, we propose a novel self-supervised meta-auxiliary learning to improve the performance of deblurring by integrating both external and internal learning. Concretely, we build a self-supervised auxiliary reconstruction task that shares a portion of the network with the primary deblurring task. The two tasks are jointly trained on an external dataset. Furthermore, we propose a meta-auxiliary training scheme to further optimize the pretrained model as a base learner, which is applicable for fast adaptation at test time. During training, the performance of both tasks is coupled. Therefore, we are able to exploit the internal information at test time via the auxiliary task to enhance the performance of deblurring. Extensive experimental results across evaluation datasets demonstrate the effectiveness of test-time adaptation of the proposed method.
Device Aging in Analog Circuits for Nanoelectronic CMOS Technologies (2012)

The trend in microelectronics to reduce device dimensions leads to increasing and new aging effects in the devices. This requires a continuous optimisation of the fabrication process as well as a degradation aware circuit design, to guarantee a high reliability of the functional elements and maintain the stable function of the system. This work focuses on the very sensitive analog circuits. Previous investigations had presented heterogeneous results. Therefore, a throughout and universal study, investigating the impact of several different aging effects on the analog circuitry, had to be performed. This work identifies highly aging sensitive circuit blocks and demonstrates the impact of device wear-out on their performance characteristics with particularly developed models and experiments. Furthermore, universal methodologies for accelerated stress measurements, aging suppression and design guidelines are proposed and evaluated.
High Density MLB using Additive and Build-up Process (1998)

A new type of printed circuit board with high density MLB (Multi-Layered printe circuit Board) has been developed for requirement of high density routing and high reliability substrates as LSI packages. The new technology has enough photo-resolution capability of Line/Space = 35μm / 35μm and 80μm diameter via hole. A full additive method is applied for the patterning, to attain the multi-layer structure. Used insulator is an unique, and newly developed photo-imagable dielectric resin which has a glass transition ≃ 200°C, peel strength ≃ 1.2kg/cm, and withstands 1000 cycles of thermal cycling (TCB). The insulator is composed of high heat resistant photo-sensitive epoxy and supper engineering plastic. This technology is suitable for direct chip attachment. This paper describes the characteristics of the new MLB.
The 25 amino acid residues at the carboxy terminus of the herpes simplex virus type 1 UL26.5 protein are required for the formation of the capsid shell around the scaffold (1995)

Herpes simplex virus type 1 (HSV-1) polypeptides specified by overlapping genes UL26 and UL26.5 form a scaffold around which the icosahedral capsid shell is assembled. In a series of cleavage events catalysed by the UL26-encoded protease, the full-length UL26 product is processed into capsid proteins VP24 and VP21 and the UL26.5 protein is converted into the capsid protein VP22a by the loss of 25 amino acids from its carboxy terminus. The roles of the UL26 and UL26.5 products were investigated using the baculovirus expression system, focusing on the function of the 25 residues cleaved from the UL26.5 protein. A key conclusion from electron microscopic analysis and protein expression studies is that the 25 amino acids at the carboxy terminus of the full-length UL26.5 protein are required for the interaction of the capsid shell proteins with the scaffold in the formation of intermediate capsids. When cells were multiply infected with baculoviruses expressing a truncated form of the UL26.5 product corresponding to VP22a and the essential components of the capsid shell, no capsids were detected, whereas large numbers of capsids were observed when the full-length UL26.5 product was used as a scaffold. The results are consistent with the proposal that cleavage of the UL26.5 product occurs after capsid assembly or when the UL26.5 protein is in a complex with one or more capsid shell proteins. Expression of VP22a in the absence or presence of capsid shell proteins resulted in the formation of large numbers of 60 nm scaffold-like particles. Since VP22a expressed from baculovirus was unable to participate in capsid assembly, these particles cannot be intermediates in the capsid assembly pathway but may be similar in structure to the protein cores present in HSV-1 immature (B) capsids.
Impaired Temperature Stress Response of a <i>Streptococcus thermophilus deoD</i> Mutant (2003)

An insertional deoD mutant of Streptococcus thermophilus strain SFi39 had a reduced growth rate at 20 degrees C and an enhanced survival capacity to heat shock compared to the wild type, indicating that the deoD product is involved in temperature shock adaptation. We report evidence that ppGpp is implicated in this dual response.