gebhart/patrepeval huggingface dataset:
An Analysis of the Relationship between Weather and Aggressive Crime in Cleveland, Ohio (2010)

Abstract This study investigated the relationship between weather and aggressive crime for the period from 1999 through 2004 for the city of Cleveland, Ohio. The majority of the analysis focused on meteorological summer (June–August), because this is the time when the most oppressive conditions occur. Citywide analysis (nonspatial) was performed for many temporal variations, which accounted for season, time of day, and day of week (weekend or weekday). The linear regression model explored the relationship between apparent temperature and aggressive crime counts. Results show that summer has the highest aggressive crime counts, while winter has the lowest crime counts. Aggressive crime generally increases linearly as apparent temperature increases, with nonaggravated assaults and domestic violence assaults having the largest response as the weather becomes hotter. The midday and early night hours (i.e., 0300–1200 LT) have the greatest significant findings relating apparent temperature to aggressive crime. Further analysis was performed at the subcity level. A threshold of mean apparent temperature of 24°C was used in order to investigate spatial patterns of aggressive crime when it is “hot” compared to when it is “cold.” Overall, the spatial patterns of crime counts are minimally influenced by hotter weather. Despite the numerous different spatial analyses that were performed, there was no significant evidence suggesting that spatial patterns of aggressive crime are greatly affected by hotter weather. Rather, it appears that warmer weather brings relatively similar percentage increases in aggressive crime activity citywide. Further exploration and analysis of the weather–crime relationship could be of significant benefit to law enforcement officials and emergency response personnel, who increasingly use geographic information system (GIS)-based tools in their work to assist in determining where and when intervention is most beneficial.
The pharmacology of N-carbamoyl-2-(2,6-dichlorophenyl)acetamidine hydrochloride (LON-954) a new tremorogenic agent. (1977)

The tremorogenic properties of a series of benzylimidoylurea derivatives are described. The most potent member, N-carbamoyl-2-(2,6-dichlorophenyl) acetamidine hydrochloride (LON-954), produces a reproducible, dose-dependent rest tremor in the mouse with oral doses of 5-100 mg/kg which is also seen in other species (rat, cat, dog, rabbit). The tremor is of constant frequency, rapid onset and short duration. It is not accompanied by akinesia, muscle ridigity, antinociceptive activity, parasympathomimetic effects or marked hypothermia and in these respects differs from tremor produced by oxotremorine. Pretreatment with a microsomal enzyme inhibitor had no effect on the tremor. An LD50 of 165 mg/kg p.o. was calculated in the mouse. After repeated administration both acute and chronic tolerance developed to the tremorogenic effects of LON-954. Evidence for a central site of action is presented, since the tremor could be reproduced following injection of small quantities (50-100 microgram) into the cerebral ventricles of the mouse. Furthermore, the use of spinal, decorticate and and decerebrate rats indicated that although tremor is not of cortical origin, it arises in an area rostral to the inferior colliculi. The mechanism underlying the tremor appears to involve dopaminergic pathways, since the action of LON-954 was antagonised by L-dopa and apomorphine and potentiated by pimozide. Atropine and carbachol were without effect. It is suggested that LON-954 could be used as an alternative to oxotremorine for the detection of anti-Parkinson drugs, particularly those exerting their effects through dopaminergic mechanisms.
Localized Amyloidosis and Alzheimer's Disease: The Rationale for Weekly Long-Term Low Dose Amyloid-Based Fractionated Radiotherapy (2008)

Amyloidosis, a plasma cell dyschrasia, is characterized by accumulation in the intercellular spaces of fibrillar proteins with a typical beta-sheet pattern. Vascular-cerebral amyloidosis is the hallmark of Alzheimer's disease and spongiform encephalopathy (Creutzfeldt-Jacob and the like). Current treatment of primary systemic amyloidosis is neither free from complications nor - in some presentations - a mortality rate. Localized tracheo-bronchial amyloidosis (TBA) has been successfully treated with high energy beams of radiation (20 Gy in 10×200 cGy in two weeks). The CT response to radiation takes several months after completion of treatment. As 20 Gy in two weeks are followed by inflammatory reactions, this dosage cannot be suggested in the treatment of amyloidotic radiosensitive organs (e.g. kidneys, liver), or in the hypothetical treatment of Alzheimer's disease. On the basis of the following points: 1) plasma cells in amyloid deposits are not numerous; 2) plasma cells are radioresistant, both in vitro and in vivo (radiotherapy of solitary plasmocytoma); 3) the effects of radiotherapy (20 Gy/2 w) on TBA localizations cannot be exclusively due to plasma cell killing, this study postulates a biophysical mechanism of radiation-induced H-bond breaks in the beta-sheet structure of amyloid, together with depolymerization of glucosaminoglycans, very radiosensitive molecules invariably associated with amyloid fibrils. As both biophysical effects are DNA-independent, the adoption of a definite time/dose ratio (e.g. 20 Gy/2 w) loses much of its importance. Therefore an innovative alternative might be a weekly long-term low-dose fractionated radiotherapy, matching the very slow response of amyloid to radiation. Before being applied to Alzheimer's disease, the proposed radiotherapy (RT) schedule should be tried in TBA patients to compare the new results of long-term fractionated RT with the old results of 20 Gy/2 w. Should long-term fractionated RT prove equally (or almost equally) effective, but certainly much less toxic than 20 Gy/2 w, its application to Alzheimer patients might become an effective and safe treatment, provided clinical and objective control by means of current imaging techniques (MRI, PET) can be assured.
A role for CD45RB<sup>low</sup> CD38<sup>+</sup> T cells and costimulatory pathways of T‐cell activation in protection of non‐obese diabetic (NOD) mice from diabetes (1999)

Non-obese diabetic (NOD) mice spontaneously develop autoimmune insulin-dependent diabetes mellitus (IDDM). Infection of the animals with mycobacteria, or immunization with mycobacteria-containing adjuvant, results in permanent protection of NOD mice from diabetes and we have recently reported that the phenomenon is associated with increased numbers of interferon-gamma-producing T cells, possessing increased cytotoxic activity, and also with augmented numbers of activated immunoglobulin M-positive (IgM+) B cells. Here, we have investigated whether protection of NOD mice from IDDM was associated with changes on costimulatory pathways of T and B cells, namely CD28/CTLA-4-B7 and CD40-CD40 ligand (CD40L) and we also further characterized protective T helper (Th) cells with regards to the expression of the differentiation markers CD45RB and CD38. We report that Th cells involved in diabetes vaccination of NOD mice by mycobacterial infection seem to belong to CD45RBlo CD38+ phenotype. The protective effect of Mycobacterium avium infection is also associated with increased CD40L and CTLA-4- expressing Th cells and with the generation of a CD40- IgG+ B cells. Our data are consistent with induction by mycobacterial infection of regulatory CD45RBlo CD38+ Th cells with the ability to trigger deletion or anergy of peripheral self-reactive lymphocytes, with shutting down of IgG+ B-cell response. They also implicate a role for IgG+ B cells in the autoimmune aggression of the endocrine pancreas of NOD mice.
Investigation of the phase diagrams of chiral praziquantel (2006)

In the present work, thermal properties of praziquantel, including melting point phase diagram and solubility, were determined for the purpose of exploration on an integrated enantioseparation process of chromatography and crystallization. The solubility of racemic praziquantel in methanol and 2-propanol was measured in the temperature range between 0 and 40 degrees C. In agreement with previous conclusions, the resulting phase diagram reveals the racemic compound behavior of praziquantel arising from the existence of eutectics. A ternary phase diagram of praziquantel enantiomers and the methanol system was also determined. Based on the information provided by the ternary solubility phase diagram, an optimized integrated enantioseparation process was suggested. Pure praziquantel crystals were recovered, and the crystal structure was solved by X-ray crystallography.
Continuous wave phase detection for probing nonlinear elastic wave interactions in rocks (1991)

A new method that uses nonlinear elastic wave generation to produce a continuous wave (cw) phase measurement from which dimensions or velocities of a body can be obtained is described. Like the technique of standing wave resonance for obtaining sound velocities, this method takes advantage of the high accuracy characteristic of frequency measurements. In the experiment, two intersecting, high-frequency primary signals f1 and f2 are mixed inside a sample, creating a directional beam at the difference frequency Δf=f1−f2. An externally generated, low-pass-filtered Δf signal is electronically mixed with the signal obtained from the sample. As either primary frequency is swept, the dc component from the mixer varies between relative maximum and minimum values at characteristic frequency intervals depending on the phase differences. The resulting interference signal can be used to calculate the distance from the mixing volume in the sample to the detector and to the two primary signal transmitters, providing that a single characteristic distance and wave velocities are known. The reverse experiment is determining velocities from known dimensions.
Joint Attention in Autonomous Driving (JAAD) (2016)

In this paper we present a novel dataset for a critical aspect of autonomous driving, the joint attention that must occur between drivers and of pedestrians, cyclists or other drivers. This dataset is produced with the intention of demonstrating the behavioral variability of traffic participants. We also show how visual complexity of the behaviors and scene understanding is affected by various factors such as different weather conditions, geographical locations, traffic and demographics of the people involved. The ground truth data conveys information regarding the location of participants (bounding boxes), the physical conditions (e.g. lighting and speed) and the behavior of the parties involved.
Model studies of the overall 5-endo-trig iodocyclization of homoallylic alcohols (1999)

Overall 5-endo-trig iodocyclizations of homoallylic alcohols, with a range of substitution patterns, leading to β-iodotetrahydrofurans are usually highly efficient and stereoselective when carried out in anhydrous acetonitrile in the presence of sodium hydrogen carbonate. Such cyclizations, which are not exceptions to Baldwin’s rules as they are electrophile-driven, appear to proceed via a well-ordered chair-like transition state. The iodine can be replaced by hydroxy, acetoxy and azide groups.
Tonoplast-localized Abc2 Transporter Mediates Phytochelatin Accumulation in Vacuoles and Confers Cadmium Tolerance (2010)

Phytochelatins mediate tolerance to heavy metals in plants and some fungi by sequestering phytochelatin-metal complexes into vacuoles. To date, only Schizosaccharomyces pombe Hmt1 has been described as a phytochelatin transporter and attempts to identify orthologous phytochelatin transporters in plants and other organisms have failed. Furthermore, recent data indicate that the hmt1 mutant accumulates significant phytochelatin levels in vacuoles, suggesting that unidentified phytochelatin transporters exist in fungi. Here, we show that deletion of all vacuolar ABC transporters abolishes phytochelatin accumulation in S. pombe vacuoles and abrogates (35)S-PC(2) uptake into S. pombe microsomal vesicles. Systematic analysis of the entire S. pombe ABC transporter family identified Abc2 as a full-size ABC transporter (ABCC-type) that mediates phytochelatin transport into vacuoles. The S. pombe abc1 abc2 abc3 abc4 hmt1 quintuple and abc2 hmt1 double mutant show no detectable phytochelatins in vacuoles. Abc2 expression restores phytochelatin accumulation into vacuoles and suppresses the cadmium sensitivity of the abc quintuple mutant. A novel, unexpected, function of Hmt1 in GS-conjugate transport is also shown. In contrast to Hmt1, Abc2 orthologs are widely distributed among kingdoms and are proposed as the long-sought vacuolar phytochelatin transporters in plants and other organisms.
Effect of Water Electrolysis Catalysts on Carbon Corrosion in Polymer Electrolyte Membrane Fuel Cells (2010)

A new approach to preventing electrochemical carbon corrosion in the cathode of polymer electrolyte membrane fuel cells (PEMFCs) was developed. The addition of 2 wt % IrO(2) (0.016 mg cm(-2)) to the catalyst layer of the cathode was demonstrated to reduce the electrochemical corrosion of carbon by 76% at 1.6 V(NHE) and 70 °C compared with a commercial Pt/C catalyst of the same Pt loading of 0.4 mg cm(-2) and under the same test conditions. The IrO(2) was shown to behave as a catalyst for water electrolysis, thereby removing water from the catalyst layer, which promoted electrochemical carbon corrosion.
Effects of changing glutamate 487 to lysine in rat and human liver mitochondrial aldehyde dehydrogenase. A model to study human (Oriental type) class 2 aldehyde dehydrogenase. (1994)

Many Oriental people possess a liver mitochondrial aldehyde dehydrogenase where glutamate at position 487 has been replaced by a lysine, and they have very low levels of mitochondrial aldehyde dehydrogenase activity. To investigate the cause of the lack of activity of this aldehyde dehydrogenase, we mutated residue 487 of rat and human liver mitochondrial aldehyde dehydrogenase to a lysine and expressed the mutant and native enzyme forms in Escherichia coli. Both rat and human recombinant aldehyde dehydrogenases showed the same molecular and kinetic properties as the enzyme isolated from liver mitochondria. The E487K mutants were found to be active but possessed altered kinetic properties when compared to the glutamate enzyme. The Km for NAD+ at pH 7.4 increased more than 150-fold, whereas kcat decreased 2-10-fold with respect to the recombinant native enzymes. Detailed steady-state kinetic analysis showed that the binding of NAD+ to the mutant enzyme was impaired, and it could be calculated that this resulted in a decreased nucleophilicity of the active site cysteine residue. The rate-limiting step for the rat E487K mutant was also different from that of the recombinant rat liver aldehyde dehydrogenase in that no pre-steady-state burst of NADH formation was found with the mutant enzyme. Both the rat native enzyme and the E487K mutant oxidized chloroacetaldehyde twice as fast as acetaldehyde, indicating that the rate-limiting step was not hydride transfer or coenzyme dissociation but depended upon nucleophilic attack. Each enzyme form showed a 2-fold activation upon the addition of Mg2+ ions. Substituting a glutamine for the glutamate did not grossly affect the properties of the enzyme. Glutamate 487 may interact directly with the positive nicotinamide ring of NAD+ for the Ki of NADH was the same in the lysine enzyme as it was in the glutamate form. Because of the altered NAD+ binding properties and kcat of the E487K variant, it is assumed that people possessing this form will not have a functional mitochondrial aldehyde dehydrogenase.
Serial fault emulation (1996)

A hardware emulator based approach has been developed to perform test evaluation on large sequential circuits (at least tens of thousands of gates). This approach relies both on the flexibility and on the reconfigurability of hardware emulators based on dedicated reprogrammable circuits. A Serial Fault Emulation (SFE) method in which each faulty circuit is emulated separately has been applied to gate-level circuits for Single Stuck Faults (SSFs).
Deconstructing (and reconstructing) cell migration (1998)

An overriding objective in cell biology is to be able to relate properties of particular molecular components to cell behavioral functions and even physiology. In the "traditional" mode of molecular cell biology, this objective has been tackled on a molecule-by-molecule basis, and in the "future" mode sometimes termed "functional genomics," it might be attacked in a high-throughput, parallel manner. Regardless of the manner of approach, the relationship between molecular-level properties and cell-level function is exceedingly difficult to elucidate because of the large number of relevant components involved, their high degree of interconnectedness, and the inescapable fact that they operate as physico-chemical entities-according to the laws of kinetics and mechanics-in space and time within the cell. Cell migration is a prominent representative example of such a cell behavioral function that requires increased understanding for both scientific and technological advance. This article presents a framework, derived from an engineering perspective regarding complex systems, intended to aid in developing improved understanding of how properties of molecular components influence the function of cell migration. That is, cell population migration behavior can be deconstructed as follows: first in terms of a mathematical model comprising cell population parameters (random motility, chemotaxis/haptotaxis, and chemokinesis/haptokinesis coefficients), which in turn depend on characteristics of individual cell paths that can be analyzed in terms of a mathematical model comprising individual cell parameters (translocation speed, directional persistence time, chemotactic/haptotactic index), which in turn depend on cell-level physical processes underlying motility (membrane extension and retraction, cell/substratum adhesion, cell contractile force, front-vs.-rear asymmetry), which in turn depend on molecular-level properties of the plethora of components involved in governance and regulation of these processes. Hence, the influence of any molecular component on cell population migration can be understood by reconstructing these relationships from the molecular level to the physical process level to the individual cell path level to the cell population distribution level. This approach requires combining experimental, theoretical, and computational methodologies from molecular biology, biochemistry, biophysics, and bioengineering.
The Antibody Targeting the E314 Peptide of Human Kv1.3 Pore Region Serves as a Novel, Potent and Specific Channel Blocker (2012)

Selective blockade of Kv1.3 channels in effector memory T (T(EM)) cells was validated to ameliorate autoimmune or autoimmune-associated diseases. We generated the antibody directed against one peptide of human Kv1.3 (hKv1.3) extracellular loop as a novel and possible Kv1.3 blocker. One peptide of hKv1.3 extracellular loop E3 containing 14 amino acids (E314) was chosen as an antigenic determinant to generate the E314 antibody. The E314 antibody specifically recognized 63.8KD protein stably expressed in hKv1.3-HEK 293 cell lines, whereas it did not recognize or cross-react to human Kv1.1(hKv1.1), Kv1.2(hKv1.2), Kv1.4(hKv1.4), Kv1.5(hKv1.5), KCa3.1(hKCa3.1), HERG, hKCNQ1/hKCNE1, Nav1.5 and Cav1.2 proteins stably expressed in HEK 293 cell lines or in human atrial or ventricular myocytes by Western blotting analysis and immunostaining detection. By the technique of whole-cell patch clamp, the E314 antibody was shown to have a directly inhibitory effect on hKv1.3 currents expressed in HEK 293 or Jurkat T cells and the inhibition showed a concentration-dependence. However, it exerted no significant difference on hKv1.1, hKv1.2, hKv1.4, hKv1.5, hKCa3.1, HERG, hKCNQ1/hKCNE1, L-type Ca(2+) or voltage-gated Na(+) currents. The present study demonstrates that the antibody targeting the E314 peptide of hKv1.3 pore region could be a novel, potent and specific hKv1.3 blocker without affecting a variety of closely related K(v)1 channels, KCa3.1 channels and functional cardiac ion channels underlying central nervous system (CNS) disorders or drug-acquired arrhythmias, which is required as a safe clinic-promising channel blocker.
High density magnetic recording on protein-derived nanoparticles (2003)

Self-organized Co/Pt nanoparticulate arrays offer a novel approach to fabricating magnetic recording media with the potential for supporting Terabit/in.2 recording densities. Protein-derived Co/Pt nanoparticles are prepared within apoferritin from aqueous reactants, with synthesis conditions controlling grain size, structure, and composition. Smooth films on glass disk substrates are produced by either spin coating or dip coating from aqueous dispersions of the precursor material. Films are typically annealed at 590 °C for 60 min with a 19 kPa (190 mBar) partial pressure of H2 to form the L10 phase. By varying the annealing conditions we are able to produce coercivities in the range of 500–8000 Oe. Electrical testing of Co/Pt nanoparticulate media using a contact test recorder shows that these nanoparticle films are capable of sustaining recording densities of more than 12.6 Gbits/in.2 (143.6 kfci, kilo flux changes per inch). In this article we will present results from finished films with regard to film structure, magnetic properties, and recording capabilities.
Nikkomycin Z against Disseminated Coccidioidomycosis in a Murine Model of Sustained-Release Dosing (2021)

Nikkomycin Z (nikZ) is a chitin synthase inhibitor. Efficacy against <i>Coccidioides</i> has been demonstrated in animal models of pulmonary or brain infection. Its short half-life in mice and in humans would necessitate divided daily dosing. We assayed nikZ efficacy in disseminated coccidioidomycosis (in a reduction of CFU design) and whether sustained release might be useful. Mice were challenged intravenously with low or high arthroconidial inocula. Fluconazole, clinically the most commonly used anticoccidioidal drug, was compared (gavage) at high dose to a dose range of nikZ administered intraperitoneally or, to mimic sustained release, administered continuously in drinking water. Therapy was given for 5 days. <i>In vitro</i>, both fluconazole and nikZ inhibited the isolate studied; nikZ was fungicidal. Oral nikZ therapy gave similar results to intraperitoneal nikZ and sterilized infection in most animals after low-inoculum challenge. In both challenges, oral nikZ produced greater reduction of CFU in organs (lung, liver, and spleen) than fluconazole. Oral nikZ doses of ≥200 mg/kg of body weight/day were particularly effective in all organs and were well tolerated. This efficacy occurred even though, after severe challenge, mice had reduced water intake, resulting in ingesting less than the desired dose, particularly initially after infection. This study shows, for the first time, efficacy of nikZ against disseminated coccidioidomycosis. Efficacy was shown after challenges producing different levels of severity of disease. This study also suggests the likely benefits of developing an extended release formulation supplying continuous systemic concentrations of nikZ.