gebhart/patrepeval huggingface dataset:
Component Monitoring of OSGi-Based Software (2010)

OSGi is a widely used service-oriented platform which provides support for the development and execution of component-based e-business applications. The component monitoring of OSGi-based software is important to the software reliability analysis and abnormality diagnosis. However, current approaches not only bring significant overhead, but also lack interaction behavior analysis. This paper focuses on monitoring components from resource utilization and interaction behavior perspectives. We propose an approach to trace thread execution for updating the relationship between threads and bundles, so that the CPU and memory utilization of bundles are calculated, and the service invocation graph is updated at runtime. A prototype tool is implemented and applied in an application server OnceAS based on OSGi. The experiments using TPC-W benchmark validate our approach without significant resource and performance overhead. Furthermore, simulation results show that our approach is of high accuracy for monitoring CPU utilization of bundles.
Improved spectral response of an InAs QD RC-SACM-APD with Ta2O5/SiO2 DBRs (2007)

We report the improvement in the spectral response of an InAs QD resonant-cavity separate absorption, charge, and multiplication avalanche photodetector (RC-SACM-APD) by increasing the quantum efficiency (QE) using dielectric Ta <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> O <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">5</sub> /SiO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> top distributed Bragg reflectors (DBRs). The reflectivities of the top and bottom DBRs were numerically designed to be 70 % and 99.1 %, respectively, in order to maximize the QE. The spectral response characteristics of the InAs QD RC-SACM-APD with enhanced top DBRs was remarkably improved at 1098 nm, which is close to the target wavelength of 1100 nm, and its full width at half-maximum (FWHM) was 19 nm. The photoluminescence (PL) spectra revealed an intense and narrow single-mode peak at 1101 nm. The FWHM of the PL peak was as narrow as 8 nm and the difference in wavelength between the spectrum and the PL peak was as small as 3 nm.
Vitamin D Receptor in Endometrial Carcinoma and the Differentiation‐Inducing Effect of 1,25‐Dihydroxyvitamin D<sub>3</sub> on Endometrial Carcinoma Cell Lines (1996)

In view of the potential of 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] as a cell-differentiation-inducing agent in endometrial cancer, the localization of the vitamin D receptor (VDR) was examined immunohistochemically in 21 endometrial adenocarcinoma specimens, and the effect of 1,25(OH)2D3 on cell growth, as well as the phenotypic changes for cell maturation after treatment with 1,25(OH)2D3, was investigated in 2 endometrial carcinoma cell lines (AMEC-1, RL95-2). The VDR was detected in 14 of the 21 endometrial carcinoma specimens. The growth of RL95-2 cells expressing VDR was inhibited to 44% when cultured with 50 nM 1,25(OH)2D3 for 6 days. In contrast, the growth of AMEC-1 cells not expressing VDR was completely uninhibited even when cultured with 100 nM 1,25(OH)2D3 for 6 days. The RL95-2 cells exposed to 50 nM 1,25(OH)2D3 for 6 days had an increasing expression for 52.5 kD or 45 kD cytokeratin polypeptide, and they became columnar with pronounced polarity and formed gland-like structures when cultured in collagen gel. These results suggest that endometrial adenocarcinoma is a target for 1,25(OH)2D3, which appears to function as a cell-differentiation-inducing agent for the treatment of endometrial cancer.
On the stability of constrained MPC without terminal constraint (2006)

The usual way to guarantee stability of model predictive control (MPC) strategies is based on a terminal cost function and a terminal constraint region. This note analyzes the stability of MPC when the terminal constraint is removed. This is particularly interesting when the system is unconstrained on the state. In this case, the computational burden of the optimization problem does not have to be increased by introducing terminal state constraints due to stabilizing reasons. A region in which the terminal constraint can be removed from the optimization problem is characterized depending on some of the design parameters of MPC. This region is a domain of attraction of the MPC without terminal constraint. Based on this result, it is proved that weighting the terminal cost, this domain of attraction of the MPC controller without terminal constraint is enlarged reaching (practically) the same domain of attraction of the MPC with terminal constraint; moreover, a practical procedure to calculate the stabilizing weighting factor for a given initial state is shown. Finally, these results are extended to the case of suboptimal solutions and an asymptotically stabilizing suboptimal controller without terminal constraint is presented.
G-Protein–Coupled Receptor Kinase Interacting Protein-1 Is Required for Pulmonary Vascular Development (2009)

Our data support a critical role for GIT1 in pulmonary vascular development by regulating VEGF-induced PLCgamma and ERK1/2 activation.
Light hypernuclei and hyperon-nucleon interaction (1990)

Light Hypernuclei are a vital testing ground for our understanding of the Hyperon-Nucleon interaction. We have performed microscopic calculations of four and five-body hypernuclei using the Nijmegen nucleon-nucleon and hyperon-nucleon interactions. Our calculations include explicit Sigma degrees of freedom. These degrees of freedom are quite important since, in contrast to the {Delta} {minus} N mass difference of {approx} 300 MeV, the {Sigma} resonance is only about 80 MeV above the {Lambda}. In addition, although there is no one-pion-exchange in the {Lambda}N diagonal channel, this longest-range term does contribute to the transition {Lambda}N {minus} {Sigma}N interaction. Our variational calculations show that the A=4 spin 0 ground state binding energy is well reproduced by the Nijmegen HN integration, a one-boson exchange model fit to the available scattering data. The spin 1 excited state and the A=5 ground state are strongly underbound, however. We demonstrate the importance of the strong tensor terms of the Nijmegen model, particularly those in the transition channel, in obtaining this result. The limited data currently available for hyperon-nucleon scattering must be greatly improved in order to place reasonable constraints on the interaction. 12 refs., 3 figs., 3 tabs.
Plasma GFAP is an early marker of amyloid-β but not tau pathology in Alzheimer’s disease (2021)

Although recent clinical trials targeting amyloid-β in Alzheimer's disease have shown promising results, there is increasing evidence suggesting that understanding alternative disease pathways that interact with amyloid-β metabolism and amyloid pathology might be important to halt the clinical deterioration. In particular, there is evidence supporting a critical role of astroglial activation and astrocytosis in Alzheimer's disease. However, so far, no studies have assessed whether astrocytosis is independently related to either amyloid-β or tau pathology in vivo. To address this question, we determined the levels of the astrocytic marker GFAP in plasma and CSF of 217 amyloid-β-negative cognitively unimpaired individuals, 71 amyloid-β-positive cognitively unimpaired individuals, 78 amyloid-β-positive cognitively impaired individuals, 63 amyloid-β-negative cognitively impaired individuals and 75 patients with a non-Alzheimer's disease neurodegenerative disorder from the Swedish BioFINDER-2 study. Participants underwent longitudinal amyloid-β (18F-flutemetamol) and tau (18F-RO948) PET as well as cognitive testing. We found that plasma GFAP concentration was significantly increased in all amyloid-β-positive groups compared with participants without amyloid-β pathology (P < 0.01). In addition, there were significant associations between plasma GFAP with higher amyloid-β-PET signal in all amyloid-β-positive groups, but also in cognitively normal individuals with normal amyloid-β values (P < 0.001), which remained significant after controlling for tau-PET signal. Furthermore, plasma GFAP could predict amyloid-β-PET positivity with an area under the curve of 0.76, which was greater than the performance achieved by CSF GFAP (0.69) and other glial markers (CSF YKL-40: 0.64, soluble TREM2: 0.71). Although correlations were also observed between tau-PET and plasma GFAP, these were no longer significant after controlling for amyloid-β-PET. In contrast to plasma GFAP, CSF GFAP concentration was significantly increased in non-Alzheimer's disease patients compared to other groups (P < 0.05) and correlated with amyloid-β-PET only in amyloid-β-positive cognitively impaired individuals (P = 0.005). Finally, plasma GFAP was associated with both longitudinal amyloid-β-PET and cognitive decline, and mediated the effect of amyloid-β-PET on tau-PET burden, suggesting that astrocytosis secondary to amyloid-β aggregation might promote tau accumulation. Altogether, these findings indicate that plasma GFAP is an early marker associated with brain amyloid-β pathology but not tau aggregation, even in cognitively normal individuals with a normal amyloid-β status. This suggests that plasma GFAP should be incorporated in current hypothetical models of Alzheimer's disease pathogenesis and be used as a non-invasive and accessible tool to detect early astrocytosis secondary to amyloid-β pathology.
Strong diameter-dependence of nanowire emission coupled to waveguide modes (2016)

The emission from nanowires can couple to waveguide modes supported by the nanowire geometry, thus governing the far-field angular pattern. To investigate the geometry-induced coupling of the emission to waveguide modes, we acquire Fourier microscopy images of the photoluminescence of nanowires with diameters ranging from 143 to 208 nm. From the investigated diameter range, we conclude that a few nanometers difference in diameter can abruptly change the coupling of the emission to a specific mode. Moreover, we observe a diameter-dependent width of the Gaussian-shaped angular pattern in the far-field emission. This dependence is understood in terms of interference of the guided modes, which emit at the end facets of the nanowire. Our results are important for the design of quantum emitters, solid state lighting, and photovoltaic devices based on nanowires.
Conducting-Interlayer SiO<sub><i>x</i></sub> Memory Devices on Rigid and Flexible Substrates (2014)

SiOx memory devices that offer significant improvement in switching performance were fabricated at room temperature with conducting interlayers such as Pd, Ti, carbon, or multilayer graphene. In particular, the Pd-interlayer SiOx memory devices exhibited improvements in lowering the electroforming voltages and threshold voltages as the number of inserted Pd layers was increased, as compared to a pure SiOx memory structure. In addition, we demonstrated that the Pd-interlayer SiOx junction fabricated on a flexible substrate maintained low electroforming voltage and mechanically stable switching properties. From these observations, a possible switching mechanism is discussed based on the formation of individual conducting paths at the weakest edge regions of each SiOx film, where the normalized bond-breaking probability of SiOx is influenced by the voltage and the thickness of SiOx. This fabrication approach offers a useful structural platform for next-generation memory applications for enhancement of the switching properties while maintaining a low-temperature fabrication method that is even amenable with flexible substrates.
Laser curing of thermosetting powder coatings: A detailed investigation (1999)

Curing of thermosetting powder coating is conventionally done in a convection or infrared oven. Powders requiring 10–15 min at cure temperature in a convection oven reportedly cure within 2–4 min in an IR oven. It has been shown that it is possible to achieve a much faster curing by using a cw CO2 laser. Laser curing has been studied as a function of laser intensity, laser beam size, and interaction time on different substrates. A comparison of laser curing with convection oven curing has been done by studying the results of time–temperature profile, ac impedance, differential scanning calorimetry, and other characterization techniques. The mechanism of laser beam interaction with the powder layer has also been discussed.
Zusammenfassung Der Ergebnisse (2004)

Diese Zusammenfassung fokussiert auf einer Auswahl der bedeutsamsten Resultate und wird in größtmöglicher Komprimierung vorgenommen.
Improved Single-Cell Proteome Coverage Using Narrow-Bore Packed NanoLC Columns and Ultrasensitive Mass Spectrometry (2020)

Single-cell proteomics can provide unique insights into biological processes by resolving heterogeneity that is obscured by bulk measurements. Gains in the overall sensitivity and proteome coverage through improvements in sample processing and analysis increase the information content obtained from each cell, particularly for less abundant proteins. Here we report on improved single-cell proteome coverage through the combination of the previously developed nanoPOTS platform with further miniaturization of liquid chromatography (LC) separations and implementation of an ultrasensitive latest generation mass spectrometer. Following nanoPOTS sample preparation, protein digests from single cells were separated using a 20 μm i.d. in-house-packed nanoLC column. Separated peptides were ionized using an etched fused-silica emitter capable of stable operation at the ∼20 nL/min flow rate provided by the LC separation. Ultrasensitive LC-MS analysis was achieved using the Orbitrap Eclipse Tribrid mass spectrometer. An average of 362 protein groups were identified by tandem mass spectrometry (MS/MS) from single HeLa cells, and 874 protein groups were identified using the Match Between Runs feature of MaxQuant. This represents an >70% increase in label-free proteome coverage for single cells relative to previous efforts using larger bore (30 μm i.d.) LC columns coupled to a previous-generation Orbitrap Fusion Lumos mass spectrometer.
Novel PPAR Pan Agonist, ZBH Ameliorates Hyperlipidemia and Insulin Resistance in High Fat Diet Induced Hyperlipidemic Hamster (2014)

Effective and safe pharmacological interventions for hyperlipidemia remains badly needed. By incorporating the key pharmacophore of fibrates into the natural scaffold of resveratrol, a novel structural compound ZBH was constructed. In present study, we found ZBH reserved approximately one third of the sirtuin 1 (SIRT1) activation produced by resveratrol at in-vitro enzyme activity assay, directly bound to and activated all three peroxisome proliferator-activated receptor (PPAR) subtypes respectively in PPAR binding and transactivation assays. Moreover, ZBH (EC₅₀, 1.75 µM) activate PPARα 21 fold more efficiently than the well-known PPAR pan agonist bezafibrate (EC₅₀ 37.37 µM) in the cellular transactivation assays. In the high fat diet induced hyperlipidemic hamsters, 5-week treatment with ZBH significantly lowered serum triglyceride, total cholesterol, LDL-C, FFA, hyperinsulinemia, and improved insulin sensitivity more potently than bezafibrate. Meanwhile, serum transaminases, creatine phosphokinase and CREA levels were found not altered by ZBH intervention. Mechanism study indicated ZBH promoted the expression of PPARα target genes and SIRT1 mRNA. Hepatic lipogenesis was markedly decreased via down-regulation of lipogenic genes, and fatty acid uptake and oxidation was simultaneously increased in the liver and skeletal muscle via up-regulation of lipolysis genes. Glucose uptake and utilization was also significantly promoted in skeletal muscle. These results suggested that ZBH significantly lowered hyperlipidemia and ameliorated insulin resistance more efficiently than bezafibrate in the hyperlipidemic hamsters primarily by activating of PPARα, and SIRT1 promotion and activation. ZBH thus presents a potential new agent to combat hyperlipidemia.
Opcode encoding for low-power instruction fetch (1999)

A method for encoding opcodes for low-power instruction fetching is described. To reduce the switching activity from opcode changes in the instruction fetch logic, opcodes are assigned so that more frequently consecutive instruction pairs have a smaller Hamming distance between their opcodes. The experimental result shows that a switching activity reduction of 37.4 – 66.7% is achievable over a naive encoding method.
Defense Responses to Tetrapyrrole-Induced Oxidative Stress in Transgenic Plants with Reduced Uroporphyrinogen Decarboxylase or Coproporphyrinogen Oxidase Activity1 (1998)

Abstract We analyzed the antioxidative defense responses of transgenic tobacco (Nicotiana tabacum) plants expressing antisense RNA for uroporphyrinogen decarboxylase or coproporphyrinogen oxidase. These plants are characterized by necrotic leaf lesions resulting from the accumulation of potentially photosensitizing tetrapyrroles. Compared with control plants, the transformants had increased levels of antioxidant mRNAs, particularly those encoding superoxide dismutase (SOD), catalase, and glutathione peroxidase. These elevated transcript levels correlated with increased activities of cytosolic Cu/Zn-SOD and mitochondrial Mn-SOD. Total catalase activity decreased in the older leaves of the transformants to levels lower than in the wild-type plants, reflecting an enhanced turnover of this photosensitive enzyme. Most of the enzymes of the Halliwell-Asada pathway displayed increased activities in transgenic plants. Despite the elevated enzyme activities, the limited capacity of the antioxidative system was apparent from decreased levels of ascorbate and glutathione, as well as from necrotic leaf lesions and growth retardation. Our data demonstrate the induction of the enzymatic detoxifying defense system in several compartments, suggesting a photosensitization of the entire cell. It is proposed that the tetrapyrroles that initially accumulate in the plastids leak out into other cellular compartments, thereby necessitating the local detoxification of reactive oxygen species.
+Your circles (2012)

Users are sharing and consuming enormous amounts of information through online social network interaction every day. Yet, many users struggle to control what they share to their overlapping social spheres. Google+ introduces circles, a mechanism that enables users to group friends and use these groups to control their social network feeds and posts. We present the results of a qualitative interview study on the sharing perceptions and behavior of 27 Google+ users. These results indicate that many users have a clear understanding of circles, using them to target information to those most interested in it. Yet, despite these positive perceptions, there is only moderate use of circles to control information flow. We explore reasons and risks associated with these behaviors and provide insight on the impact and open questions of this privacy mechanism.
Many Routes to an Antibody Heavy-Chain CDR3: Necessary, Yet Insufficient, for Specific Binding (2018)

Because of its great potential for diversity, the immunoglobulin heavy-chain complementarity-determining region 3 (HCDR3) is taken as an antibody molecule's most important component in conferring binding activity and specificity. For this reason, HCDR3s have been used as unique identifiers to investigate adaptive immune responses <i>in vivo</i> and to characterize <i>in vitro</i> selection outputs where display systems were employed. Here, we show that many different HCDR3s can be identified within a target-specific antibody population after <i>in vitro</i> selection. For each identified HCDR3, a number of different antibodies bearing differences elsewhere can be found. In such <i>selected</i> populations, all antibodies with the same HCDR3 recognize the target, albeit at different affinities. In contrast, within <i>unselected</i> populations, the majority of antibodies with the same HCDR3 sequence do not bind the target. In one HCDR3 examined in depth, all target-specific antibodies were derived from the same VDJ rearrangement, while non-binding antibodies with the same HCDR3 were derived from many different V and D gene rearrangements. Careful examination of previously published <i>in vivo</i> datasets reveals that HCDR3s shared between, and within, different individuals can also originate from rearrangements of different V and D genes, with up to 26 different rearrangements yielding the same identical HCDR3 sequence. On the basis of these observations, we conclude that the same HCDR3 can be generated by many different rearrangements, but that specific target binding is an outcome of unique rearrangements and VL pairing: the HCDR3 is necessary, albeit insufficient, for specific antibody binding.
Alginate–chitosan micro- and nanoparticles for transmucosal delivery of proteins (2016)

The properties of protein-containing micro- and nanoparticles that were produced from alginate and chitosan using the methods of layer-by-layer polyelectrolyte adsorption and ionotropic gelation have been compared. The encapsulation efficiency of proteins (aprotinin, interferon, and human insulin), the size and ζ-potential of the particles, the mucin binding, and the protein release under physiological conditions have been studied. The prospects for the possible mucosal application of the particles are discussed.
A Myristoylated Cell-Penetrating Peptide Bearing a Transferrin Receptor-Targeting Sequence for Neuro-Targeted siRNA Delivery (2014)

Many neurodegenerative disorders (NDDs) are characterized by aggregation of aberrant proteins and extensive oxidative stress in brain cells. As a treatment option for NDDs, RNA interference (RNAi) is a promising approach to suppress the activation of abnormal genes and negative regulators of antioxidant genes. Efficient neuro-targeted siRNA delivery requires a delicate optimization of nucleic acid carriers, quite distinct from putative pDNA carriers in regard to stable condensation and serum protection of siRNA, blood-brain barrier (BBB) bypass, effective siRNA delivery to brain cells, and functional release of bioactive siRNA at therapeutic levels. Here, we propose that a myristic acid conjugated, cell-penetrating peptide (transportan; TP), equipped with a transferrin receptor-targeting peptide (myr-TP-Tf), will lead to stable encapsulation of siRNA and targeted delivery of siRNA to brain cells overcoming the BBB. Myr-TP-Tf was successfully prepared by solid-phase peptide synthesis with high purity. Myr-TP-Tf-siRNA complexes formulated at a 20:1 (peptide-siRNA) molar ratio provided prolonged siRNA stability against serum and ribonuclease treatment. Fluorescence images clearly indicated that siRNA uptake was successfully achieved by myr-TP-Tf complexes in both a murine brain endothelioma and a human glioma cell line. The luciferase assay and the human placental alkaline phosphatase (hPAP) reporter assay results demonstrated the functional gene silencing effect of myr-TP-Tf-siRNA complexes in a human glioma cell line as well as in primary murine neurons/astrocytes, supportive of successful release of bioactive siRNA into the cytosol. Finally, the transcytosis assay revealed that favorable siRNA transport via receptor-mediated transcytosis was mediated by myr-TP-Tf complexes. In summary, these data suggest that myr-TP-Tf peptides possess promising properties as a vehicle for neuro-targeted siRNA delivery. We will further study this peptide in vitro and in vivo for transport mechanism kinetics and to validate its capability to deliver siRNA to the brain, respectively.