gebhart/patrepeval huggingface dataset:
Liquid-liquid phase separation of light-inducible transcription factors increases transcription activation in mammalian cells and mice (2021)

Light-inducible gene switches represent a key strategy for the precise manipulation of cellular events in fundamental and applied research. However, the performance of widely used gene switches is limited due to low tissue penetrance and possible phototoxicity of the light stimulus. To overcome these limitations, we engineer optogenetic synthetic transcription factors to undergo liquid-liquid phase separation in close spatial proximity to promoters. Phase separation of constitutive and optogenetic synthetic transcription factors was achieved by incorporation of intrinsically disordered regions. Supported by a quantitative mathematical model, we demonstrate that engineered transcription factor droplets form at target promoters and increase gene expression up to fivefold. This increase in performance was observed in multiple mammalian cells lines as well as in mice following in situ transfection. The results of this work suggest that the introduction of intrinsically disordered domains is a simple yet effective means to boost synthetic transcription factor activity.
An active mail system (1984)

Conventional electronic mail is passive text that is created, sent and read. Any further actions must be initiated by the recipient. By contrast, an "intelligent message" (imessage) is an active program that carries on a dialogue with the recipient. The imessage may subsequently route itself to other users as a result of the responses it receives, and it eventually returns these responses to the original sender.This paper describes a prototype intelligent mail system and discusses some of the problems involved in implementing such a system in a distributed environment.
High-efficiency, flexible CdTe solar cells on ultra-thin glass substrates (2015)

Flexible, high-efficiency, low-cost solar cells can enable applications that take advantage of high specific power, flexible form factors, lower installation and transportation costs. Here, we report a certified record efficiency of 16.4% for a flexible CdTe solar cell that is a marked improvement over the previous standard (14.05%). The improvement was achieved by replacing chemical-bath-deposited CdS with sputtered CdS:O and also replacing the high-temperature sputtered ZnTe:Cu back contact layer with co-evaporated and rapidly annealed ZnTe:Cu. We use quantum efficiency and capacitance-voltage measurements combined with device simulations to identify the reasons for the increase in efficiency. Both device simulations and experimental results show that higher carrier density can quantitatively account for the increased open circuit voltage (VOC) and Fill Factor (FF), and likewise, the increase in short circuit current density (JSC) can be attributed to the more transparent CdS:O.
KETAMINE FOR TREATMENT-RESISTANT UNIPOLAR AND BIPOLAR MAJOR DEPRESSION: CRITICAL REVIEW AND IMPLICATIONS FOR CLINICAL PRACTICE (2016)

There is an urgent need for more rapidly effective pharmacotherapies for major depressive disorder and bipolar disorder (BP) that are efficacious and tolerable for depressed patients who respond poorly to conventional treatments. Multiple controlled trials have now demonstrated a rapid, nonsustained antidepressive response to a single intravenous infusion of ketamine. Early controlled studies of intranasal or serial infusion therapy appear promising. The effective dose for depression is lower than the typical anesthetic doses, and side-effects are generally mild and transient. The data investigating the adjunctive use of concurrent ketamine in the course of electroconvulsive therapy (ECT) for depression do not suggest efficacy or tolerability. The therapeutic potential of ketamine has stimulated considerable excitement among clinicians, patients, and industry, and has led to the increasing use of ketamine as an off-label substitute for ECT and other antidepressive treatments. This clinical review of ketamine will assess the evidence-based use of ketamine and initial clinical implications of further development of a potentially novel treatment for rapid reduction of symptoms in depressed patients.
Evidence for the Identity of Murine Gamma Interferon and Macrophage Activating Factor (1982)

Macrophages activating factor (MAF) in mouse lymphokine preparations was quantitated using a tumor cell cytotoxicity assay. MAF activity was compared with gamma interferon (IFN-gamma) activity, and the lymphokine mixture subjected to a variety of protein fractionation procedures. No significant difference in the ratio of MAF activity to IFN activity was observed following any of the fractionation steps, even after MAF had been purified to a specific activity of 1 X 10(6) u/mg protein. Gel permeation using high pressure liquid chromatography showed a coincident peak of MAF and IFN activity at approximately 55 kD. Both activities were reduced at similar rates following heating at 56 degrees C or incubation at 4 degrees C in pH 2 buffer. Finally, induction of lymphokines using different inducers (mitogens or antigens) or cell populations always resulted in similar ratios of MAF activity to IFN activity. These results support the hypothesis that MAF and IFN-gamma are identical proteins.
Sphenopalatine (nasal) ganglion: remote effects including "psychosomatic" symptoms, rage reaction, pain, and spasm. (1979)

Many articles implicate the nasal ganglion in the production of remote symptoms and discuss treatment. Symptoms are primarily spastic, involving both visceral and voluntary muscles including muscle spasm in the neck, shoulder, and low back; asthma, hypertension, intestinal spasm; diarrhea, angina pectoris, uterine spasm; intractable hiccup, and many others. All these symptoms appear to have 2 common denominators. They are mediated by the autonomic nervous system and at least in some instances can be "psychosomatic." The sphenopalatine ganglion (SPG) is a major autonomic ganglion located superficially in the pterygopalatine fossa, with major afferent distribution to the entire nasopharynx and important connections with the trigeminal nerve, facial nerve, internal carotid artery plexus of the sympathetic nervous system and, as shown in the rat, direct connection with the anterior pituitary gland. This paper presents arguments supporting the following hypotheses: 1. The SPG probably has a crucial role in lower animals in declenching the reflex responses known collectively as the rage reaction. 2. The SPG is a major point of entry to the autonomic system exposed to pathologic influences and readily accessible for therapeutic influences and readily accessible for therapeutic intervention. 3. A wide variety of symptoms are produced or maintained by alteration in autonomic system tonus and some of these may be affected by intervention on the SPG. 4. The possible relationship of some symptoms and "psychosomatic" conditions to the autonomic nervous system and the rage reaction must be considered.20
Stability of quercetin derivatives in vacuum impregnated apple slices after drying (microwave vacuum drying, air drying, freeze drying) and storage (2014)

Microwave vacuum drying (MVD) was investigated for apple slices enriched with quercetin derivatives by vacuum impregnation (VI). Additional freeze drying (FD) and air drying (AD) were conducted. Compared to native apples, the impregnated tissue resulted in higher moisture content, elevation of weight and significant browning, due to the incorporated VI solution. The total quercetin content and quercetin glycoside composition were not affected by MVD and FD. The vacuum conditions protect the polyphenols from oxygen dependent degradation and browning reactions. AD resulted in an average quercetin glycoside loss of 44% and undesirable changes, particularly discoloration. The degradation is caused by both non-enzymatic and enzymatic reactions. The pulsed microwave energy intake improved the drying result in structure and led to a faster drying process of 130 min. The bulk density of MVD apple chips (0.69 g/ml) ranged between 0.33 g/ml for FD and 0.75 g/ml for AD. The final moisture content was the lowest after FD (6.8 g/100 g), followed by 9.0 g/100 g after MVD and 12.7 g/100 g after AD. The shelf life was significantly influenced by storage temperature and time. After 12 month at 20 °C, the total quercetin content decreased by 21%.
Ultralow-cost deformable mirror based on thermal expansion (2003)

Low-cost adaptive optics is applied in lasers, scientific instrumentation, ultrafast sciences and ophthalmology. These applications demand the deformable mirrors to be simple, inexpensive, reliable and efficient. We report on a novel type of ultra-low-cost deformable mirror with thermal actuators. The device has response time of ~5 s, actuator stroke of about 6 μm, temporal stability of about λ/10 <i>rms</i> in the visible range and can be used for correction of rather large aberrations with slow changing amplitude.
Tetramer Bence Jones Protein in the Immunoproliferative Diseases: Angioimmunoblastic Lymphadenopathy, Primary Amyloidosis, and Multiple Myeloma (1989)

The authors report the clinical features and the results of immunochemical studies of patients with angioimmunoblastic lymphadenopathy, primary amyloidosis, and multiple myeloma, each of whom had in the serum and urine multiple forms of Bence Jones protein (BJP). The BJPs were isolated and purified and were shown by electrophoretic, gel filtration, and ultracentrifugal analyses to exist as tetramers and dimers. The components in two cases were kappa type and in one lambda type. The kappa tetramers consisted of two covalent dimers and the lambda tetramer two noncovalently bound dimers present in both serum and urine.
Application and performance of downlink beamforming techniques in umts (2003)

The opportunities and constraints for application of beamforming techniques in UMTS are reviewed and compared by means of extensive dynamic network-level simulations. We start by reviewing the physical layer specifications for UMTS in order to clarify which channels are allowed to use beamforming. Second, radio resource management for cells with beamforming capabilities are addressed from a standardization and algorithmic point of view. The exchange of beamforming specific measurement reports between the base station (Node-B) and the radio network controller is discussed as well as beam switching mechanisms, directional power-based admission control, and more. In addition to implementation of beamforming within logical cells, options for exploiting the antenna array to synthesize independent sector beams are also investigated (i.e., higher order sectorization via beamforming). The presented results show that beamforming within logical cells is an effective enabler for higher system capacity, while the use of beamforming for creation of many logical cells is less attractive due to significantly larger soft handover overhead and somewhat reduced capacity gain.
Automatic updating method based on Maven (2014)

Traditional software updating and maintenance are generally free to the software development process. Based on Maven, a sophisticated software build tool, this paper presents an automatic update method: using POM model of Maven in user terminal management and update all dependent modules of the software, thus completing the entire software updates. This method makes the software update be incorporated into the Maven project management processes, enabling seamless continuous, automatic software build and update from source code changes to the terminal user. Experiments show that this method is feasible, effective.
Bi-Sr-Ca-Cu-oxide superconducting thin films deposited by dc magnetron sputtering (1988)

Thin films of the high-temperature superconductor Bi-Sr-Ca-Cu-oxide were deposited by dc magnetron sputtering onto various substrates. Films deposited on (100) MgO substrates achieved zero resistance between 78 and 82.5 K depending upon the annealing conditions. The best films were produced with a quick post-anneal in oxygen at 870 °C followed by a slower anneal in air at the same temperature. The composition of the sputtered films was uniform within ±3% over an area 40 mm in radius, and films deposited at 15 and 40 mm from the center of deposition both exhibited high Tc transitions.
Study of Semi-supervised Approaches to Improving English-Mandarin Code-Switching Speech Recognition (2018)

In this paper, we present our overall efforts to improve the performance of a code-switching speech recognition system using semi-supervised training methods from lexicon learning to acoustic modeling, on the South East Asian Mandarin-English (SEAME) data. We first investigate semi-supervised lexicon learning approach to adapt the canonical lexicon, which is meant to alleviate the heavily accented pronunciation issue within the code-switching conversation of the local area. As a result, the learned lexicon yields improved performance. Furthermore, we attempt to use semi-supervised training to deal with those transcriptions that are highly mismatched between human transcribers and ASR system. Specifically, we conduct semi-supervised training assuming those poorly transcribed data as unsupervised data. We found the semi-supervised acoustic modeling can lead to improved results. Finally, to make up for the limitation of the conventional n-gram language models due to data sparsity issue, we perform lattice rescoring using neural network language models, and significant WER reduction is obtained.
p53‐independent Induction of p21 (WAF1/CIP1), Reduction of Cyclin B1 and G2/M Arrest by the Isoflavone Genistein in Human Prostate Carcinoma Cells (2000)

Genistein, a natural isoflavonoid phytoestrogen, is a strong inhibitor of protein tyrosine kinase and DNA topoisomerase II activities. Genistein has been shown to have anticancer proliferation, differentiation and chemopreventive effects. In the present study, we have addressed the mechanism of action by which genistein suppressed the proliferation of p53-null human prostate carcinoma cells. Genistein significantly inhibited the cell growth, which effect was reversible, and induced dendrite-like structure. The inhibitory effects of genistein on cell growth proliferation were associated with a G2/M arrest in cell cycle progression concomitant with a marked inhibition of cyclin B1 and an induction of Cdk inhibitor p21 (WAF1/CIP1) in a p53-independent manner. Following genistein treatment of cells, an increased binding of p21 with Cdk2 and Cdc2 paralleled a significant decrease in Cdc2 and Cdk2 kinase activity with no change in Cdk2 and Cdc2 expression. Genistein also induced the activation of a p21 promoter reporter construct, utilizing a sequence distinct from the p53-binding site. Analysis of deletion constructs of the p21 promoter indicated that the response to genistein could be localized to the 300 base pairs proximal to the transcription start site. These data suggest that genistein may exert a strong anticarcinogenic effect, and that this effect possibly involves an induction of p21, which inhibits the threshold kinase activities of Cdks and associated cyclins, leading to a G2/M arrest in the cell cycle progression.
(Invited) Multilevel Cell Nonvolatile Memory with Field-effect Transistor Using Crystalline Oxide Semiconductor (2014)

Multilevel cell nonvolatile memory has been developed with a field-effect transistor (FET) containing a crystalline oxide semiconductor (OS), specifically c-axis aligned crystalline In-Ga-Zn-oxide (CAAC-IGZO). The memory is called nonvolatile oxide semiconductor random access memory (NOSRAM). A NOSRAM cell consists of a CAAC-IGZO FET, a p-channel MOS (PMOS) transistor, and a cell capacitor. We have demonstrated that a single 3-bit NOSRAM cell has an endurance of 10 12 cycles and a write time of 5 ns. The 6σ tolerance for cell V th distribution of a test chip including an 18-kbit NOSRAM cell array is 109 mV. The test chip achieves a 3-bit/cell NOSRAM.
Novel method for structured light system calibration (2006)

System calibration, which usually involves complicated and time-consuming procedures, is crucial for any 3-D shape measurement system. In this work, a novel systematic method is proposed for accurate and quick calibration of a 3-D shape measurement system we developed based on a structured light technique. The key concept is to enable the projector to "capture" images like a camera, thus making the calibration of a projector the same as that of a camera. With this new concept, the calibration of structured light systems becomes essentially the same as the calibration of traditional stereovision systems, which is well established. The calibration method is fast, robust, and accurate. It significantly simplifies the calibration and recalibration procedures of structured light systems. This work describes the principle of the proposed method and presents some experimental results that demonstrate its performance.
Cholesterol absorption is mainly regulated by the jejunal and ileal ATP-binding cassette sterol efflux transporters Abcg5 and Abcg8 in mice (2004)

In the present study, we investigated whether intestinal sterol efflux transporters Abcg5 and Abcg8 play a major role in determining variations in cholesterol (Ch) absorption efficiency, and we compared the physiological functions of the duodenal, jejunal, and ileal Abcg5 and Abcg8 on the absorption of Ch and sitostanol in inbred mice challenged with various amounts of Ch, sitostanol, hydrophilic, or hydrophobic bile acids. We found that Abcg5 and Abcg8 in the jejunum and ileum, but not in the duodenum, were main factors in determining, in part, variations in Ch absorption efficiency. The jejunal and ileal Abcg5 and Abcg8 played a major regulatory role in response to high dietary cholesterol and were more sensitive in the regulation of Ch absorption when compared with sitostanol absorption. These results, combined with different sterol uptake rates, suggest that the absorption efficiency of Ch and sitostanol is determined by the net results between influx and efflux of intraluminal Ch and sitostanol molecules crossing the apical membrane of the enterocyte. Hydrophilic and hydrophobic bile acids influenced Ch absorption through mediating Ch solubilization and its physical-chemical state within the small intestinal lumen. We conclude that Ch absorption is mainly regulated by the jejunal and ileal Abcg5 and Abcg8 in mice.
Empathic communities (1998)

article Free Access Share on Empathic communities: reaching out across the Web Author: Jenny Preece Univ. of Maryland, Baltimore Univ. of Maryland, BaltimoreView Profile Authors Info & Claims InteractionsVolume 5Issue 2March/April 1998 pp 32–43https://doi.org/10.1145/274430.274435Published:01 March 1998Publication History 77citation1,537DownloadsMetricsTotal Citations77Total Downloads1,537Last 12 Months64Last 6 weeks7 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my Alerts New Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
The Fundamentals of Electromagnetic Waves (2016)

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Realization of a uniform circular source using a two-dimensional binary filter (1985)

A uniform-intensity circular light source has been realized from a Gaussian laser beam by using an area-modulated binary filter. The technique preserves the wave front, and as a result the uniform circular source can be located at a plane perpendicular to the axis of propagation over a wide area.
Adversarial Attacks on Face Detectors Using Neural Net Based Constrained Optimization (2018)

Adversarial attacks involve adding, small, often imperceptible, perturbations to inputs with the goal of getting a machine learning model to misclassifying them. While many different adversarial attack strategies have been proposed on image classification models, object detection pipelines have been much harder to break. In this paper, we propose a novel strategy to craft adversarial examples by solving a constrained optimization problem using an adversarial generator network. Our approach is fast and scalable, requiring only a forward pass through our trained generator network to craft an adversarial sample. Unlike in many attack strategies we show that the same trained generator is capable of attacking new images without explicitly optimizing on them. We evaluate our attack on a trained Faster R-CNN face detector on the cropped 300-W face dataset where we manage to reduce the number of detected faces to 0.5% of all originally detected faces. In a different experiment, also on 300-W, we demonstrate the robustness of our attack to a JPEG compression based defense typical JPEG compression level of 75% reduces the effectiveness of our attack from only 0.5% of detected faces to a modest 5.0%.
The use of gaze to control drones (2014)

This paper presents an experimental investigation of gaze-based control modes for unmanned aerial vehicles (UAVs or "drones"). Ten participants performed a simple flying task. We gathered empirical measures, including task completion time, and examined the user experience for difficulty, reliability, and fun. Four control modes were tested, with each mode applying a combination of x-y gaze movement and manual (keyboard) input to control speed (pitch), altitude, rotation (yaw), and drafting (roll). Participants had similar task completion times for all four control modes, but one combination was considered significantly more reliable than the others. We discuss design and performance issues for the gaze-plus-manual split of controls when drones are operated using gaze in conjunction with tablets, near-eye displays (glasses), or monitors.
Evidence for autotetraploidy associated with reproductive isolation in <i>Saccharomyces cerevisiae</i>: towards a new domesticated species (2009)

Partial or whole-genome duplications have played a major role in the evolution of new species. We have investigated the variation of ploidy level in a panel of domesticated strains of Saccharomyces cerevisiae coming from different geographical origins. Segregation studies and crosses with tester strains of different ploidy levels showed that part of the strains were well-balanced autotetraploids displaying tetrasomic inheritance. The presence of up to four different alleles for various loci is consistent with a polyploidization mechanism relying on the fusion of two nonreduced meiospores coming from two S. cerevisiae strains. Autotetraploidy was also in accordance with karyotype and flow cytometry analyses. Interestingly, most bakery strains were tetraploids, suggesting a link between ploidy level and human use. The null or drastically reduced fertility of the hybrids between tetraploid and diploid strains indicated that domesticated S. cerevisiae strains are composed of two groups isolated by post-zygotic reproductive barriers.