gebhart/patrepeval huggingface dataset:
Vasoactive Intestinal Peptide Potentiates Sexual Behavior: Inhibition by Novel Antagonist* (1989)

Vasoactive intestinal peptide (VIP) has been suggested as a neurotransmitter mediating penile erection. We now show that VIP can stimulate sexual behavior in rats with reduced masculine potential due to pituitary grafting or castration. This effect was attenuated in the presence of a novel VIP antagonist, devised by a hybrid peptide strategy. Thus, we have synthesized a molecule combining a portion of VIP with a portion of neurotensin, peptides of opposite pharmacological action on cAMP formation and smooth muscle relaxation. The hybrid peptide markedly inhibited VIP's effect on sexual behavior. This inhibition was manifested by a significant increase in the mean interval between copulatory events (greater than 3-fold change) coupled with a blockade of VIP-stimulated ejaculation. Other putative VIP antagonists were not as effective in blocking these activities. Thus, our results imply that VIP is not only associated with penile erection, but is involved in sexual behavior as well. Furthermore, the hybrid antagonist was shown to inhibit VIP binding in glial cell cultures. The availability of highly potent VIP antagonists may offer a route to study the possible multiple VIP receptors as well as help delineate other biological activities attributable to VIP.
Viewpoint-Aware Attentive Multi-view Inference for Vehicle Re-identification (2018)

Vehicle re-identification (re-ID) has the huge potential to contribute to the intelligent video surveillance. However, it suffers from challenges that different vehicle identities with a similar appearance have little inter-instance discrepancy while one vehicle usually has large intra-instance differences under viewpoint and illumination variations. Previous methods address vehicle re-ID by simply using visual features from originally captured views and usually exploit the spatial-temporal information of the vehicles to refine the results. In this paper, we propose a Viewpoint-aware Attentive Multi-view Inference (VAMI) model that only requires visual information to solve the multi-view vehicle reID problem. Given vehicle images of arbitrary viewpoints, the VAMI extracts the single-view feature for each input image and aims to transform the features into a global multiview feature representation so that pairwise distance metric learning can be better optimized in such a viewpointinvariant feature space. The VAMI adopts a viewpoint-aware attention model to select core regions at different viewpoints and implement effective multi-view feature inference by an adversarial training architecture. Extensive experiments validate the effectiveness of each proposed component and illustrate that our approach achieves consistent improvements over state-of-the-art vehicle re-ID methods on two public datasets: VeRi and VehicleID.
Congenital Central Hypoventilation Syndrome (2006)

These data suggest that nonpolyalanine repeat mutations produce more severe disruption of PHOX2B function. Patients carrying these mutations should be evaluated for HSCR and neural crest tumors. Because incomplete penetrance can occur in families of CCHS probands with PHOX2B mutations, genetic screening of appropriate family members is indicated to evaluate reproductive risk and because asymptomatic mutation carriers may be at risk for developing alveolar hypoventilation.
Mutant HSP70 Reverses Autoimmune Depigmentation in Vitiligo (2013)

Vitiligo is an autoimmune disease characterized by destruction of melanocytes, leaving 0.5% of the population with progressive depigmentation. Current treatments offer limited efficacy. We report that modified inducible heat shock protein 70 (HSP70i) prevents T cell-mediated depigmentation. HSP70i is the molecular link between stress and the resultant immune response. We previously showed that HSP70i induces an inflammatory dendritic cell (DC) phenotype and is necessary for depigmentation in vitiligo mouse models. Here, we observed a similar DC inflammatory phenotype in vitiligo patients. In a mouse model of depigmentation, DNA vaccination with a melanocyte antigen and the carboxyl terminus of HSP70i was sufficient to drive autoimmunity. Mutational analysis of the HSP70i substrate-binding domain established the peptide QPGVLIQVYEG as invaluable for DC activation, and mutant HSP70i could not induce depigmentation. Moreover, mutant HSP70iQ435A bound human DCs and reduced their activation, as well as induced a shift from inflammatory to tolerogenic DCs in mice. HSP70iQ435A-encoding DNA applied months before spontaneous depigmentation prevented vitiligo in mice expressing a transgenic, melanocyte-reactive T cell receptor. Furthermore, use of HSP70iQ435A therapeutically in a different, rapidly depigmenting model after loss of differentiated melanocytes resulted in 76% recovery of pigmentation. Treatment also prevented relevant T cells from populating mouse skin. In addition, ex vivo treatment of human skin averted the disease-related shift from quiescent to effector T cell phenotype. Thus, HSP70iQ435A DNA delivery may offer potent treatment opportunities for vitiligo.
Operation of a separated-type x-ray interferometer for phase-contrast x-ray imaging (1999)

Aiming at large-area phase-contrast x-ray imaging, a separated-type x-ray interferometer system was designed and developed to produce 25×20 mm interference patterns. The skew-symmetric optical system was adopted because of the feasibility of alignment. The rotation between the separated crystal blocks was controlled within a drift of 0.06 nrad using a feedback positioning system. This interferometer generated a 25×15 mm interference pattern with 0.07 nm synchrotron x-rays. A slice of a rabbit’s kidney was observed, and its tubular structure could be revealed in a measured phase map.
Free-form sketching with variational implicit surfaces (2002)

With the advent of sketch-based methods for shape construction, there's a new degree of power available in the rapid creation of approximate shapes. Sketch [Zeleznik, 1996] showed how a gesture-based modeler could be used to simplify conventional CSG-like shape creation. Teddy [Igarashi, 1999] extended this to more free-form models, getting much of its power from its ``inflation'' operation (which converted a simple closed curve in the plane into a 3D shape whose silhouette, from the current point of view, was that curve on the view plane) and from an elegant collection of gestures for attaching additional parts to a shape, cutting a shape, and deforming it. But despite the powerful collection of tools in Teddy, the underlying polygonal representation of shapes intrudes on the results in many places. In this paper, we discuss our preliminary efforts at using variational implicit surfaces [Turk, 2000] as a representation in a free-form modeler. We also discuss the implementation of several operations within this context, and a collection of user-interaction elements that work well together to make modeling interesting hierarchies simple. These include ``stroke inflation'' via implicit functions, blob-merging, automatic hierarchy construction, and local surface modification via silhouette oversketching. We demonstrate our results by creating several models. Categories and Subject Descriptors (according to ACM CCS): I.3.5 [Computer Graphics]: Modeling packages I.3.6 [Computer Graphics]: Interaction techniques
Palmitoylated PrRP analog decreases body weight in DIO rats but not in ZDF rats (2016)

Anorexigenic neuropeptides produced and acting in the brain have the potential to decrease food intake and ameliorate obesity, but are ineffective after peripheral application, owing to a limited ability to cross the blood-brain barrier. We have designed lipidized analogs of prolactin-releasing peptide (PrRP), which is involved in energy balance regulation as demonstrated by obesity phenotypes of both Prrp-knockout and Prrp receptor-knockout mice. The aim of this study was to characterize the subchronic effect of a palmitoylated PrRP analog in two rat models of obesity and diabetes: diet-induced obese Sprague-Dawley rats and leptin receptor-deficient Zucker diabetic (ZDF) rats. In the rats with diet-induced obesity (DIO), a two-week intraperitoneal treatment with palmitoylated PrRP lowered food intake by 24% and body weight by 8%. This treatment also improved glucose tolerance and tended to decrease leptin levels and adipose tissue masses in a dose-dependent manner. In contrast, in ZDF rats, the same treatment with palmitoylated PrRP lowered food intake but did not significantly affect body weight or glucose tolerance, probably in consequence of severe leptin resistance due to a nonfunctional leptin receptor. Our data indicate a good efficacy of lipidized PrRP in DIO rats. Thus, the strong anorexigenic, body weight-reducing, and glucose tolerance-improving effects make palmitoylated PrRP an attractive candidate for anti-obesity treatment.
Tuning Hydrogel Properties to Promote the Assembly of Salivary Gland Spheroids in 3D (2016)

Current treatments for chronic xerostomia, or "dry mouth", do not offer long-term therapeutic benefits for head and neck cancer survivors previously treated with curative radiation. Towards the goal of creating tissue-engineered constructs for the restoration of salivary gland functions, we developed new hyaluronic acid (HA)-based hydrogels using thiolated HA (HA-SH) and acrylated HA (HA-AES) with a significant molecular weight mismatch. Four hydrogel formulations with varying HA concentration, (1-2.4 wt%) and thiol/acrylate ratios (2/1 to 36/1) and elastic moduli (G': 35 to 1897 Pa, 2 h post-mixing) were investigated. In our system, thiol/acrylate reaction was initiated rapidly upon mixing of HA-SH/HA-AES to establish thioether crosslinks with neighboring ester groups, and spontaneous sulfhydryl oxidation occurred slowly over several days to install a secondary network. The concurrent reactions cooperatively create a cell-permissive network to allow for cell expansion and aggregation. Multicellular spheroids formed readily from a robust ductal epithelial cell line (Madin-Darby Canine Kidney, MDCK cells) in all hydrogel formulations investigated. Primary salivary human stem/progenitor cells (hS/PCs), on the other hand, are sensitive to the synthetic extracellular environment, and organized acini-like structures with an average diameter of 50 µm were obtained only in gels with G' ≤ 216 Pa and a thiol/acrylate ratio ≥18. The spheroid size and size distribution were dependent on the HA content in the hydrogel. Cells in hS/PC spheroids formed tight junctions (occludin), remained viable and proliferative, secreted structural proteins (collagen IV and laminin) found in the basement membrane and maintained key stem/progenitor markers. We conclude that incorporation of time-dependent, dynamic features into a covalently crosslinked HA network produces an adaptable hydrogel framework that promotes hS/PC assembly and supports early aspects of salivary morphogenesis, key to reconstitution of a fully functional implantable salivary gland.
CD4+ T cells containMycobacterium tuberculosis infection in the absence of CD8+ T cells in mice vaccinated with DNA encoding Ag85A (2000)

The contribution of CD8+ and CD4+ T cell-mediated effector functions against Mycobacterium tuberculosis infection elicited by i.m. vaccination with plasmid DNA encoding the immunodominant Ag85A antigen of M. tuberculosis was studied. Ag85A DNA-vaccinated beta2-microglobulin gene-deficient (beta2m-/-) mice, which lack CD8+ T cells, produced Ag85-specific antibodies and Th1 type cytokines similar to wild-type mice. Although beta2m-/- mice were more susceptible to M. tuberculosis infection, following vaccination they efficiently controlled bacterial replication in spleen and lungs 4 weeks post-infection. In contrast, mice lacking CD4+ T cells were neither sensitized by the Ag85A DNA vaccine to produce Ag85-specific antibodies or Th1 type cytokines nor did they contain a M. tuberculosis challenge infection. In addition, Ag85A DNA-vaccinated IFN-gamma gene knockout mice produced Ag85-specific antibodies and IL-2 but died rapidly following a M. tuberculosis challenge infection. Collectively, these data support the view that IFN-gamma-producing CD4+ T cells, independently of CD8+ T cells, may mediate the protective effect of the Ag85A DNA vaccine.
Polyimide Cellulose Nanocrystal Composite Aerogels (2014)

Cellulose nanocrystals derived from\ntunicates (<i>t</i>-CNC) were used as a reinforcing nanofiller\nfor polyimide aerogels.\nTwo sets of polyimide aerogels, containing either 2,2′-dimethyl­benzidine\n(DMBZ) or 4,4′-oxydianiline (ODA) and 3,3′,4,4′-biphenyl­tetracarboxylic\nacid dianhydride (BPDA) cross-linked with 1,3,5-tris­(4-amino­phenoxy)­benzene\n(TAB), were studied. Total solids composition of the aerogels were\nkept constant at 7.5 wt %, with 0–13.33 wt % of the total solids\nbeing the carboxylic acid-functionalized <i>t</i>-CNC (<i>t</i>-CNC–COOH) filler. The incorporation of the <i>t</i>-CNC–COOH, with carboxylic acid content of either\n560 or 920 mmol/kg, in the polyimide aerogel networks improved both\nphysical and mechanical properties of the final materials. Isothermal\naging of <i>t</i>-CNC–COOH aerogel composites was\nalso conducted at 150 and 200 °C for 24 h. Higher content <i>t</i>-CNC–COOH/polyimide aerogels showed less change\nin their density and reduced shrinkage during aging, which further\nemphasized the effect of the <i>t</i>-CNC–COOH reinforcement\nin retaining the structural integrity of the aerogel.
Enhanced production of enveloped viruses in BST‐2‐deficient cell lines (2017)

Despite all the advantages that cell-cultured influenza vaccines have over egg-based influenza vaccines, the inferior productivity of cell-culture systems is a major drawback that must be addressed. BST-2 (tetherin) is a host restriction factor which inhibits budding-out of various enveloped viruses from infected host cells. We developed BST-2-deficient MDCK and Vero cell lines to increase influenza virus release in cell culture. BST-2 gene knock-out resulted in increased release of viral particles into the culture medium, by at least 2-fold and up to 50-fold compared to release from wild-type counterpart cells depending on cell line and virus type. The effect was not influenza virus/MDCK/Vero-specific, but was also present in a broad range of host cells and virus families; we observed similar results in murine, human, canine, and monkey cell lines with viruses including MHV-68 (Herpesviridae), influenza A virus (Orthomyxoviridae), porcine epidemic diarrhea virus (Coronaviridae), and vaccinia virus (Poxviridae). Our results suggest that the elimination of BST-2 expression in virus-producing cell lines can enhance the production of viral vaccines. Biotechnol. Bioeng.2017;114: 2289-2297. © 2017 Wiley Periodicals, Inc.
The Fe-B-C ternary amorphous alloys (1980)

The properties of amorphous Fe-B-C alloys have been studied. The replacement of boron by carbon reduces the thermal stability of alloys containing more than approximately 80-percent iron, increases the Curie temperature slightly, and decreases the slope of the magnetization-temperature curve. Thus, although the low-temperature saturation magnetization decreases on replacement of B by C, as expected, the room-temperature magnetization exhibits a broad ridge of constant saturation magnetization per gram extending from approximately Fe <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">80</inf> B <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">20</inf> to approximately Fe <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">83</inf> B <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">11</inf> C <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">6</inf> . This ridge follows one of the low-temperature magnetization contours. The density of the alloys in the series Fe <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">84</inf> B <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">16-x</inf> C <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">x</inf> was measured and compared to calculated densities. The increase in density agrees with the calculated values and results in a slight increase in saturation magnetization per unit volume with increase in carbon. The coercivity, both as-cast and after stress relief annealing, increases on replacement of B by C. This is not understood.
METHOD FOR CALIBRATING THE IMAGE FROM A MIXEL CAMERA BASED SOLELY ON THE ACQUIRED HYPERSPECTRAL DATA (2013)

The mixel camera combines a new type of hardware component – an array of light mixing chambers – with a mathematical method that restores captured hyperspectral data with large keystone to its keystone-free form. When it is no longer necessary to correct keystone in hardware, the requirements to the optical design become much less stringent, and the mixel camera can therefore collect about four times more light than most traditional high-resolution cameras. However, for the mathematical data restoring method to function correctly, the geometry of the camera – such as the relative position of the image sensor and the slit – should be known with a small fraction of a pixel precision. Due to quite small sensor pixel size, it may be very challenging to make the camera so rigid mechanically that previously obtained calibration data remain valid for a long enough period. We will in this paper show how the captured hyperspectral data from the scene of interest, i.e., an unknown natural scene, can be used to give sufficiently precise calibration.
Supporting Information for: A highly efficient form of the selenocysteine insertion sequence element in protozoan parasites and its use in mammalian cells (2007)

Selenoproteins are an elite group of proteins containing a rare amino acid, selenocysteine (Sec), encoded by the codon, UGA. In eukaryotes, incorporation of Sec requires a Sec insertion sequence (SECIS) element, a stem–loop structure located in the 3'-untranslated regions of selenoprotein mRNAs. Here we report identification of a noncanonical form of SECIS element in Toxoplasma gondii and Neospora canine, single-celled apicomplexan parasites of humans and domestic animals. This SECIS has a GGGA sequence in the SBP2-binding site in place of AUGA previously considered invariant. Using a combination of computational and molecular techniques, we show that Toxoplasma and Neospora possess both canonical and noncanonical SECIS elements. The GGGA-type SECIS element supported Sec insertion in mammalian HEK 293 and NIH 3T3 cells and did so more efficiently than the natural mammalian SECIS elements tested. In addition, mammalian type I and type II SECIS elements mutated into the GGGA forms were functional but manifested decreased Sec insertion efficiency. We carried out computational searches for both AUGA and GGGA forms of SECIS elements in Toxoplasma and detected five selenoprotein genes, including one coding for a previously undescribed selenoprotein, designated SelQ, and two containing the GGGA form of the SECIS element. In contrast, the GGGA-type SECIS elements were not detected in mammals and nematodes. As a practical outcome of the study, we developed pSelExpress1, a vector for convenient expression of selenoproteins in mammalian cells. It contains an SBP2 gene and the most efficient tested SECIS element: an AUGA mutant of the GGGA-type Toxoplasma SelT structure.
Physical compensation of phase curvature in digital holographic microscopy by use of programmable liquid lens (2015)

Quantitative phase measurements obtained with digital holographic microscopes are strongly dependent on the optical arrangement of the imaging system. The nontelecentric operation provides phase measurements affected by a parabolic phase factor and requires numerical postprocessing, which does not always remove all the perturbation. Accurate phase measurements are achieved by using the imaging system in telecentric mode. Unfortunately, this condition is not accomplished when a commercial microscope is used as the imaging system. In this paper, we present an approach for obtaining accurate phase measurements in nontelecentric imaging systems without the need for numerical postprocessing. The method uses an electrically tunable liquid lens to illuminate the sample so that the perturbing parabolic wavefront is cancelled out. Experimental holograms of a Fresnel lens and a section of the thorax of a Drosophila melanogaster fly are captured to verify the proposed method.
A rapid means of separating A14-/sup 125/I-insulin from heterogeneously labeled insulin molecules for biologic studies (1982)

We have used two methods for the preparation of a highly homogeneous insulin with high specific activity. After iodination with chloramine T, the labeled peptides were retained on a disposable Sep Pak cartridge and subsequently eluted. The eluted labeled insulins were further purified by either DEAE cellulose or high performance liquid chromatography (HPLC) to separate A14-/sup 125/I- from A19-/sup 125/I-insulin. Both methods of chromatography were effective, but HPLC offered the advantage of better resolution in less time and higher yields of A14-/sup 125/I-insulin, which is suitable for biologic studies in various target tissues.
Microalgae Filtration Using an Electrochemically Reactive Ceramic Membrane: Filtration Performances, Fouling Kinetics, and Foulant Layer Characteristics (2020)

Electrochemical membrane filtration has proven to be successful for microbial removal and separation from water. In addition, membrane fouling could be mitigated by electrochemical reactions and electrostatic repulsion on a reactive membrane surface. This study assessed the filtration performances and fouling characteristics of electrochemically reactive ceramic membranes (a Magneli phase suboxide of TiO<sub>2</sub>) when filtering algal suspension under different dc currents to achieve anodic or cathodic polarization. The critical flux results indicate that when applying positive or negative dc currents (e.g., 1.25-2.5 mA·cm<sup>-2</sup>) to the membrane, both significantly mitigated membrane fouling and thus maintained higher critical fluxes (up to 14.6 × 10<sup>-5</sup>·m<sup>3</sup>·m<sup>-2</sup>·s<sup>-1</sup> or 526 LMH) compared to the critical flux without dc currents. Moreover, applying dc currents also enhanced membrane defouling processes and recovered high permeate flux better than hydraulic and chemical backwash methods. Moreover, fouling kinetics and the cake layer formation were further analyzed with a resistance-in-series model that revealed many important but underexamined parameters (e.g., cake layer resistance and cake layer thickness). The cake layer structures (e.g., compressibility) were shown to vary with the electrochemical activity, which provide new insight into the biofouling mechanisms. Finally, the algogenic odor, geosmin, was shown to be effectively removed by this reactive membrane under positive dc currents (2.5 mA·cm<sup>-2</sup>), which highlights the multifunctional capabilities of electrochemically reactive membrane filtration in biomass separation, fouling prevention, and pollutant degradation.