gebhart/patrepeval huggingface dataset:
Eye tracking off the shelf (2004)

What if eye trackers could be downloaded and used immediately with standard cameras connected to a computer, without the need for an expert to setup the system? This has already the case for head trackers, so why not for eye trackers?Using components off-the-shelf (COTS) for camera-based eye tracking tasks has many advantages, but it certainly introduces several new problems as less assumptions on the system can be made. As a consequence of using COTS the price for eye tracking devices can be reduced while increasing the accessibility of these systems. Eye tracking based on COTS holds potential for a large number of possible applications such as in the games industry and eye typing [Majaranta and Räihä 2002]. Different cameras may be purchased depending on the need and the amount of money the user is willing to spend on the camera. In this framework it is not possible to use IR light sources and other novel engineered devices as they cannot be bought in a common hardware store. Very little control over the cameras and the geometry of the setup can be expected. The methods employed for eye tracking should therefore be able to handle changes in light conditions and image defocusing and scale changes [Hansen and Pece 2003]. On the same token pan-and-tilt cameras cannot be used, thus forcing such systems to be passive. Figure 1 shows a possible setup of a COTS-based eye tracker. When designing systems for the general public, it is unrealistic to assume that people are able to do camera calibration and make accurate setups of camera, monitor and user. Since little is known about the setup, would this then require a vast amount of calibration points needed for gaze estimation? That is, how many calibration points are really needed? Obviously the more calibration points are used the better the chances are to be able to infer the mapping from the image to gaze direction. It would even be possible to sample the entire function space provided sufficiently many calibration points are given. From the point of view of the users, a low number of calibration points is preferred as calibration may be considered as a tedious procedure. Systems that require many calibration points for every session are therefore not likely to succeed. It is also important to know the accuracy in gaze determination when using COTS to determine their applicability for various tasks.
Automated Characterization of Pigment Epithelial Detachment by Optical Coherence Tomography (2011)

Automated classification of PEDs using internal reflectivity characteristics appears to be sensitive for detecting serous and fibrovascular PEDs. Automated classification and quantification of PEDs may be a useful tool in future studies for stratifying PEDs according to risk and possibly predicting the risk of advanced AMD.
Topical administration of nanocarrier miRNA‐210 antisense ameliorates imiquimod‐induced psoriasis‐like dermatitis in mice (2019)

Psoriasis is a chronic, recurrent inflammatory autoimmune skin disease. Although its etiology and pathogenesis are complex and multifarious, it has been proved to be closely related to dysregulation of immune cell function as well as keratinocyte proliferation/differentiation. Our previous study demonstrated that miRNA-210 (miR-210) plays an important role in the formation of skin lesions and immune imbalance in psoriasis. Here, we developed a biomimetic reconstituted high-density lipoprotein (rHDL) nanocarrier gel containing miR-210 antisense (NG-anti-miR-210) to investigate its effect on imiquimod (IMQ)-induced psoriasis-like dermatitis in mice. We found that topical treatment with NG-anti-miR-210 significantly decreased the expression of miR-210 in both the skin lesions and splenic CD4<sup>+</sup> T cells from IMQ-induced psoriasis-like mouse models and ameliorated the dermatitis in terms of the erythema, scales, acanthosis and dermal inflammatory cell infiltration in IMQ-induced mice. In addition, the proportion of T-helper (Th)1 and Th17 cells in dermal and splenic cells of IMQ-treated mice were decreased by application of NG-anti-miR-210, accompanied by decreased interleukin-17A and γ-interferon mRNA levels. Therefore, our data demonstrate that topical inhibition of miR-210 delivered by rHDL nanocarrier effectively alleviates the psoriasis-like inflammation in mice and provides a potentially effective topical drug for psoriasis.
A LysM Receptor-Like Kinase Plays a Critical Role in Chitin Signaling and Fungal Resistance in <i>Arabidopsis</i> (2008)

Chitin, a polymer of N-acetyl-d-glucosamine, is found in fungal cell walls but not in plants. Plant cells can perceive chitin fragments (chitooligosaccharides) leading to gene induction and defense responses. We identified a LysM receptor-like protein (LysM RLK1) required for chitin signaling in Arabidopsis thaliana. The mutation in this gene blocked the induction of almost all chitooligosaccharide-responsive genes and led to more susceptibility to fungal pathogens but had no effect on infection by a bacterial pathogen. Additionally, exogenously applied chitooligosaccharides enhanced resistance against both fungal and bacterial pathogens in the wild-type plants but not in the mutant. Together, our data indicate that LysM RLK1 is essential for chitin signaling in plants (likely as part of the receptor complex) and is involved in chitin-mediated plant innate immunity. The LysM RLK1-mediated chitin signaling pathway is unique, but it may share a conserved downstream pathway with the FLS2/flagellin- and EFR/EF-Tu-mediated signaling pathways. Additionally, our work suggests a possible evolutionary relationship between the chitin and Nod factor perception mechanisms due to the similarities between their potential receptors and between the signal molecules perceived by them.
Heterostructure photonic crystals: theory and applications (2002)

A hybrid structure combining square and hexagonal photonic crystal lattices is presented. This structure, which we refer to as heterostructure, offers the ability to tailor, optimize, and match the band structure of different lattices. The availability of heterostructures in photonic crystals opens a broad range of possibilities for optical device development. In particular, heterostructure photonic crystals are well suited for the application of optical beam splitting (Y coupler) and combining. Numerical experiments performed by use of the finite-difference time-domain method are shown to illustrate the device implemented in both unistructure and heterostructure lattices.
ERCC1 and ERCC2 Expression in Malignant Tissues From Ovarian Cancer Patients (1992)

We conclude that ERCC1 expression levels in human tumor tissue may have a role in clinical resistance to platinum compounds. These data appear to be consistent with the assertion that ERCC1 serves as an excision nuclease, whereas ERCC2 serves as a helicase.
Biophysical activity of an artificial surfactant containing an analogue of surfactant protein (SP)-C and native SP-B (1999)

Natural surfactant preparations containing phospholipids and the hydrophobic surfactant proteins B and C (SP-B and SP-C) are effective in the treatment of respiratory distress syndrome in premature infants. The limited supply, and the risk of infectious agents and immunological reactions have promoted the evaluation of synthetic peptides in surfactant preparations. However, the folding of synthetic SP-C into an α-helix is inefficient and α-helical SP-C analogues with Val → Leu substitutions form oligomers. In order to circumvent these problems we have synthesized an SP-C analogue, named SP-C(LKS), which differs from SP-C mainly by the exchange of most of the Val residues in positions 16–28 with Leu residues to promote an α-helical conformation, and by the introduction of Lys residues at positions 17, 22 and 27 in order to locate positive charges around the helical circumference and thereby avoid self polymerization. CD spectroscopy showed a spectrum typical for α-helical peptides and SDS/PAGE disclosed a single band. The biophysical activity of artificial surfactant preparations containing SP-C(LKS) and phospholipids, with and without native SP-B, was measured using a Wilhelmy balance and a pulsating bubble surfactometer. SP-C(LKS) (3%, w/w) in a mixture of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC)/phosphatidylglycerol/palmitic acid (68:22:9, by wt.) suspended in 150 mM NaCl, showed rapid spreading at the air-liquid interface and produced a surface tension of < 1 mN/m at minimum bubble size (γmin) and 42 mN/m at maximum bubble size (γmax) in the pulsating bubble surfactometer. The addition of 2% (w/w) SP-B to the preparation reduced the maximum surface tension to 33–35 mN/m, i.e. both γmin and γmax values were similar to those of natural surfactant preparations. Optimal in vitro characteristics were also obtained from a preparation containing SP-C(LKS), SP-B, DPPC and phosphatidylglycerol, i.e. when palmitic acid was omitted from the lipid mixture. SP-B containing surfactant preparations made up in Hepes buffer at pH 6.9, instead of in 150 mM NaCl, had similar biophysical activity provided that palmitic acid was omitted, but decreased activity in the presence of palmitic acid.
Augmented B Lymphocyte Response to Antigen in the Absence of Antigen-Induced B Lymphocyte Signaling in an IgG-Transgenic Mouse Line (2010)

IgG-containing B cell antigen receptor (IgG-BCR), the BCR mostly expressed on memory B cells, contains a distinct signaling function from IgM-BCR or IgD-BCR expressed on naïve B cells. Because naïve B cells transgenic for IgG exhibit augmented response to antigens similar to memory B cells, the distinct signaling function of IgG-BCR appears to play a role in augmented antibody responses of memory B cells. However, how IgG-BCR signaling augments B cell responses is not yet well understood. Here we demonstrate that B cells from IgG-transgenic mice are anergic with defect in generation of BCR signaling upon BCR ligation. However, these IgG-transgenic B cells generate markedly augmented antibody response to a T cell-dependent antigen, probably due to hyper-responsiveness to a T cell-derived signal through CD40. Both BCR signaling defect and augmented response to CD40 ligation are partially restored in xid IgG-transgenic mice in which BCR signaling is down-modulated due to a loss-of-function mutation in the tyrosine kinase Btk crucial for BCR signaling. Thus, IgG-BCR induces augmented B cell responses in the absence of antigen-induced BCR signaling probably through high ligand-independent BCR signaling that may "idle" B cells to make them ready to respond to T cell help. This finding strongly suggests a crucial role of ligand-independent signaling in receptor function.
Characteristics of a hybrid type 3-phase stepping motor (1998)

Among hybrid type (HB type) stepping motors, the 2-phase type is widely used, as is the 5-phase type for high-resolution purposes. On the other hand, the 3-phase type is considered to be superior to other types from the viewpoint of cost performance. However, no study has been made of 3-phase HB type stepping motors. In this paper, the performance characteristics of an HB type 3-phase stepping motor are analyzed, and, further, the influence of motor structure and of excitation modes on the motor performance are also investigated. As a result, the relationship between number of stator main poles and number of rotor teeth is formulated. Torque versus angle characteristics, step response characteristics, and pullout torque characteristics are clarified under the various excitation modes in Y- and Δ-winding connections. © 1998 Scripta Technica. Electr Eng Jpn, 123(3): 46–54, 1998
Joint Base-Calling of Two DNA Sequences With (2010)

Automated estimation of DNA base-sequences is an important step in genomics and in many other emerging fields in biological and medical sciences. Current automated sequencers process single strands only. To improve the utility of existing technologies, we propose to mix two independent strands prior to electrophoresis, and base-call jointly by applying the sum-product algorithm on factor graphs. We first present a statistical model for DNA sequencing data and examine the model parameters. A practical heuristic is then proposed to estimate the peaks, which are then separated into two source sequences (Major/Minor) by passing messages on a factor graph. Simulation results show that joint base-calling can provide less accurate but valid results for the minor. The algorithm presented provides a basis for future investigation of joint sequencing techniques.
Syntheses of telechelic oligo‐ and poly(carboxypiperazine)s (1986)

Abstract Poly(carboxypiperazine) is only accessible via low temperature polycondensation methods. The melting point of the polymer is higher than the temperature of its decomposition. Oligomers with piperidine end groups were prepared to determine the melting points of the pure polymer by extrapolation from the melting points of the oligomers. The decomposition temperatures were determined by thermogravimetry. These studies led to the design of meltable telechelics.
[Development for a novel cancer vaccine]. (2004)

Clinical trial using epitope peptides have been intensively performed in the world. From the results, it has been clarified that tumor specific CTLs were induced in the vaccinated cancer patients. In this manuscript, we demonstrated the problems to be clarified from clinical trial and recent advances for cancer vaccine using epitope peptides.
Peripheral nerve regeneration by microbraided poly(L‐lactide‐<i>co</i>‐glycolide) biodegradable polymer fibers (2003)

Tiny tubes with fiber architecture were developed by a novel method of fabrication upon introducing some modification to the microbraiding technique, to function as nerve guide conduit and the feasibility of in vivo nerve regeneration was investigated through several of these conduits. Poly(L-lactide-co-glycolide) (10:90) polymer fibers being biocompatible and biodegradable were used for the fabrication of the conduits. The microbraided nerve guide conduits (MNGCs) were characterized using scanning electron microscopy to study the surface morphology and fiber arrangement. Degradation tests were performed and the micrographs of the conduit showed that the degradation of the conduit is by fiber breakage indicating bulk hydrolysis of the polymer. Biological performances of the conduits were examined in the rat sciatic nerve model with a 12-mm gap. After implantation of the MNGC to the right sciatic nerve of the rat, there was no inflammatory response. One week after implantation, a thin tissue capsule was formed on the outer surface of the conduit, indicating good biological response of the conduit. Fibrin matrix cable formation was seen inside the MNGC after 1 week implantation. One month after implantation, 9 of 10 rats showed successful nerve regeneration. None of the implanted tubes showed tube breakage. The MNGCs were flexible, permeable, and showed no swelling apart from its other advantages. Thus, these new poly(L-lactide-co-glycolide) microbraided conduits can be effective aids for nerve regeneration and repair and may lead to clinical applications.
Refractive-Index-Matched Indium–Tin-Oxide Electrodes for Liquid Crystal Displays (2009)

Refractive-index-matched indium-tin-oxide (ITO) electrode for thin-film transistor liquid crystal displays is presented to reduce optical losses caused by Fresnel reflections. Simulations show a 24% improvement in optical transmittance when the conventional dense ITO is replaced with the refractive-index-matched ITO in a stack of glass/ITO/liquid crystal/ITO/glass, The refractive-index-matched ITO, fabricated by oblique-angle deposition technique, shows higher optical transmittance and smaller dependency on film thickness and wavelength than conventional dense ITO. (c) 2009 The Japan Society of Applied Physics
Disrupted Adenovirus-Based Vaccines Against Small Addictive Molecules Circumvent Anti-Adenovirus Immunity (2012)

Adenovirus (Ad) vaccine vectors have been used for many applications due to the capacity of the Ad capsid proteins to evoke potent immune responses, but these vectors are often ineffective in the context of pre-existing anti-Ad immunity. Leveraging the knowledge that E1(-)E3(-) Ad gene transfer vectors are potent immunogens, we have developed a vaccine platform against small molecules by covalently coupling analogs of small molecules to the capsid proteins of disrupted Ad (dAd5). We hypothesized that the dAd5 platform would maintain immunopotency even in the context of anti-Ad neutralizing antibodies. To test this hypothesis, we coupled cocaine and nicotine analogs, GNE and AM1, to dAd5 capsid proteins to generate dAd5GNE and dAd5AM1, respectively. Mice were pre-immunized with Ad5Null, resulting in high titer anti-Ad5 neutralizing antibodies comparable to those observed in the human population. The dAd5GNE and dAd5AM1 vaccines elicited high anti-cocaine and anti-nicotine antibody titers, respectively, in both naive and Ad5-immune mice, and both functioned to prevent cocaine or nicotine from reaching the brain of anti-Ad immune mice. Thus, disrupted Ad5 evokes potent humoral immunity that is effective in the context of pre-existing neutralizing anti-Ad immunity, overcoming a major limitation for current Ad-based vaccines.
Distributed Reverse DNS Geolocation (2018)

IP geolocation databases map IP addresses to their geographical locations. These databases are used in a variety of online services to serve local content to users. Here we present methods for extracting locations from the reverse DNS hostnames assigned to IP addresses. We summarize a machine learning based approach which, given a hostname, aims to extract and rank potential location candidates, which can then potentially be fused with other geolocation signals. We show that this approach significantly outperforms a state-of-the-art academic baseline, and it is competitive and complementary to commercial baselines. Since extracting locations from more than a billion reverse DNS hostnames at once poses a significant computational challenge, we develop a distributed version of our algorithm. We perform experiments on a cluster of 2,000 machines to demonstrate that our distributed implementation can scale. We show that compared to the single machine version, our distributed approach can achieve a speedup of more than 150×.
Batch production of single-crystal diamond bridges and cantilevers for microelectromechanical systems (2010)

The utilization of single-crystal diamond offers a way to achieve extreme semiconductor and mechanical properties of diamond for microelectromechanical systems (MEMS). However, current diamond MEMS devices are limited to polycrystalline or nanocrystalline films. In this paper, we report on the batch fabrication and mechanical operation of single-crystal diamond bridges/cantilevers. The bending of these bridges/cantilevers is achieved by nanoindentation with atomic force microscopy. The resonant vibration of the cantilevers is demonstrated by using a piezoelectric actuation method with direct optical readout. These bridges/cantilevers exhibit high-quality single crystal. The maximum measured Young's modulus of the diamond bridges/cantilevers is more than 1000 GPa. The air gap between the diamond bridges/cantilevers and the substrate guarantees the application of single-crystal diamond to the MEMS.
A High-Performance Rechargeable Iron Electrode for Large-Scale Battery-Based Energy Storage (2012)

Inexpensive, robust and efficient large-scale electrical energy storage systems are vital to the utilization of electricity generated from solar and wind resources. In this regard, the low cost, robustness, and eco-friendliness of aqueous iron-based rechargeable batteries are particularly attractive and compelling. However, wasteful evolution of hydrogen during charging and the inability to discharge at high rates have limited the deployment of iron-based aqueous batteries. We report here new chemical formulations of the rechargeable iron battery electrode to achieve a ten-fold reduction in the hydrogen evolution rate, an unprecedented charging efficiency of 96%, a high specific capacity of 0.3 Ah/g, and a twenty-fold increase in discharge rate capability. We show that modifying high-purity carbonyl iron by in situ electro-deposition of bismuth leads to substantial inhibition of the kinetics of the hydrogen evolution reaction. The in situ formation of conductive iron sulfides mitigates the passivation by iron hydroxide thereby allowing high discharge rates and high specific capacity to be simultaneously achieved. These major performance improvements are crucial to advancing the prospect of a sustainable large-scale energy storage solution based on aqueous iron-based rechargeable batteries.
Completion of the Sequence of the Genome of the Coronavirus Avian Infectious Bronchitis Virus (1987)

The nucleotide sequence determination of the genome of the Beaudette strain of the coronavirus avian infectious bronchitis virus (IBV) has been completed. The complete sequence has been obtained from 17 overlapping cDNA clones, the 5'-most of which contains the leader sequence (as determined by direct sequencing of the genome) and the 3'-most of which contains the poly(A) tail. Approximately 8 kilobases at the 3' end of this sequence have already been published. These contain the sequences of mRNAs A to E within which are the genes for the spike, the membrane and the nucleocapsid polypeptides: the main structural components of the virion. The remainder of the sequence, equivalent to the 'unique' region of mRNA F, is some 20 kilobases in length and is thought to code for a polymerase or polymerases which are involved in the replication of the genome and the production of the subgenomic messenger RNAs. This sequence contains two large open reading frames, potentially coding for polypeptides of molecular weights 441,000 and 300,000. Unlike other large open reading frames in the virus, the 300,000 open reading frame appears to have no subgenomic RNA associated with it which would allow it to be at the 5' end of an mRNA species. Because of this, and because of the characteristics of the sequence in the region immediately upstream of its start codon, other mechanisms of translation, such as ribosome slippage, must be postulated.
Assessment of camera phone distortion and implications for watermarking (2011)

The paper presents a watermark robustness model based on the mobile phone camera's spatial frequency response and watermark embedding parameters such as density and strength. A new watermark robustness metric based on spatial frequency response is defined. The robustness metric is computed by measuring the area under the spatial frequency response for the range of frequencies covered by the watermark synchronization signal while excluding the interference due to aliasing. By measuring the distortion introduced by a particular camera, the impact on watermark detection can be understood and quantified without having to conduct large-scale experiments. This in turn can provide feedback on adjusting the watermark embedding parameters and finding the right trade-off between watermark visibility and robustness to distortion. In addition, new devices can be quickly qualified for their use in smart image applications. The iPhone 3G, iPhone 3GS, and iPhone 4 camera phones are used as examples in this paper to verify the behavior of the watermark robustness model.