gebhart/patrepeval huggingface dataset:
Pernicious anaemia as a risk factor for osteoporosis (1992)

1. If gastric acid is necessary for the absorption of dietary calcium, the total absence of gastric acid secretion that occurs in pernicious anaemia could result in bone loss. To investigate this, we measured calcium absorption and bone density in 21 postmenopausal women (ages 51-76 years) with pernicious anaemia and in 24 normal postmenopausal women (ages 51-79 years). 2. Relative to the normal women, in the women with pernicious anaemia the bone mineral density of the lumbar spine was decreased by 16% (P less than 0.001). 3. After adjustment for age and body weight, lumbar spine bone mineral density correlated with the serum concentration of group 1 pepsinogens (a group of proteins produced by the gastric fundus) (r = 0.61, P less than 0.01). 4. Despite achlorhydria, the women with pernicious anaemia had normal true fractional calcium absorption and normal serum levels of parathyroid hormone and 1,25-dihydroxyvitamin D. 5. We conclude that gastric acid is not required for the absorption of dietary calcium. Thus, the loss of cancellous bone must be caused by some mechanism yet to be identified.
Distributed model of peritoneal fluid absorption (2006)

The process of water reabsorption from the peritoneal cavity into the surrounding tissue substantially decreases the net ultrafiltration in patients on peritoneal dialysis. The goal of this study was to propose a mathematical model based on data from clinical studies and animal experiments to describe the changes in absorption rate, interstitial hydrostatic pressure, and tissue hydration caused by increased intraperitoneal pressure after the initiation of peritoneal dialysis. The model describes water transport through a deformable, porous tissue after infusion of isotonic solution into the peritoneal cavity. Blood capillary and lymphatic vessels are assumed to be uniformly distributed within the tissue. Starling's law is applied for a description of fluid transport through the capillary wall, and the transport within the interstitium is modeled by Darcy's law. Transport parameters such as interstitial fluid volume ratio, tissue hydraulic conductance, and lymphatic absorption in the tissue are dependent on local interstitial pressure. Numerical simulations show the strong dependence of fluid absorption and tissue hydration on the values of intraperitoneal pressure. Our results predict that in the steady state only approximately 20-40% of the fluid that flows into the tissue from the peritoneal cavity is absorbed by the lymphatics situated in the tissue, whereas the larger (60-80%) part of the fluid is absorbed by the blood capillaries.
Intrathecal Administration of Mesenchymal Stem Cells Reduces the Reactive Oxygen Species and Pain Behavior in Neuropathic Rats (2014)

These results suggest that rMSCs may modulate neuropathic pain generation through ROS expression after spinal nerve ligation.
Simple and Efficient Microwave-Assisted Hydrogenation Reactions at Moderate Temperature and Pressure (2006)

A generally applicable method for the introduction of gaseous hydrogen into a sealed reaction system to perform hydrogenation reactions under microwave irradiation has been developed. Several different types of substrates are easily reduced in short reaction times with moderate temperatures between 80 °C and 100 °C with 50 psi of hydrogen. The use of simultaneous cooling is also applied to the hydrogenation of more difficult substrates.
Synthesis of Y 2 O 3 -MgO nanopowder and infrared transmission of the sintered nanocomposite (2008)

A sol-gel method was successfully used to synthesize Y<sub>2</sub>O<sub>3</sub>-MgO composite powder with a grain size below 30nm. Results from X-ray diffraction revealed how the microstructure evolved with increasing heat treating temperature. Taking advantage of high heating rate, spark plasma sintering (SPS) technique was used to sinter the nanopowder, resulting in a fully dense nanocomposite with grain size below 100nm. The transmittance can be enhanced by optimizing sintering parameters as well as appropriate post-sinter annealing. The fully consolidated nanocomposite exhibits an optical transmittance between 75% and 84% over infrared wavelength range between 3μm and 6μm. This kind of nanocomposite has a great potential to be used as infrared transparent material.
Combining Protein-Based IMAC, Peptide-Based IMAC, and MudPIT for Efficient Phosphoproteomic Analysis (2008)

Immobilized metal affinity chromatography (IMAC) is a common strategy used for the enrichment of phosphopeptides from digested protein mixtures. However, this strategy by itself is inefficient when analyzing complex protein mixtures. Here, we assess the effectiveness of using protein-based IMAC as a pre-enrichment step prior to peptide-based IMAC. Ultimately, we couple the two IMAC-based enrichments and MudPIT in a quantitative phosphoproteomic analysis of the epidermal growth factor pathway in mammalian cells identifying 4470 unique phosphopeptides containing 4729 phosphorylation sites.
Localization of varicella-zoster virus nucleic acids and proteins in human skin (1995)

The pathogenic mechanisms involved in varicella-zoster virus (VZV) infections remain elusive. The pattern of cutaneous distribution of the IE63 protein and of the gpI (gE) and gpII glycoproteins with their corresponding genome sequences during VZV infections was studied by immunohistochemistry and in situ hybridization. Skin biopsy specimens were obtained from immunocompetent and immunocompromised patients with varicella, herpes zoster, or atypical VZV lesions. The first evidence for VZV infection consisted of the presence of IE63 in keratinocytes. In the vesicles and pustules, the viral transcripts gpI, gpII, and IE63 and the corresponding nucleic acids for gpI and gpII were identified in keratinocytes, sebocytes, Langerhans cells, dermal dendrocytes, monocytes/macrophages, and endothelial cells. The gpI and gpII glycorpoteins were essentially located on the cellular membranes while IE63 expression was generally restricted to the nuclei. In three biopsies of early herpes zoster, viral proteins were disclosed in dermal nerves and in perineurial type I dendrocytes. This was never encountered in varicella. Vasculitic changes and endothelial cell involvement were more prominent in varicella than in herpes zoster. It is concluded that the secondary viremia in varicella that affects the dermal endothelial cells is followed by a cell-to-cell spread to keratinocytes. In herpes zoster, the viral progression through cutaneous nerves primarily extends to the pilosebaceous units with a secondary involvement of epidermal keratinocytes, followed by a further spread to dermal cells.
Varactor-loaded transmission-line phase shifter at C -band using lumped elements (2003)

The design of varactor-loaded transmission-line phase shifters using lumped elements is discussed in this paper. A monolithic-microwave integrated-circuit (MMIC) phase shifter is fabricated to verify the proposed topology. Only one control voltage is required for phase control. Within a continuously adjustable phase-control range of 360/spl deg/ and a frequency range from 5 to 6 GHz, a low transmission loss of 4 dB/spl plusmn/1.7 dB is measured. The phase shifter is realized with a commercial 0.6-/spl mu/m GaAs MESFET process and requires a chip area of only 0.8 mm/sup 2/. To the knowledge of the authors, the best results reported to date are reached for a continuously adjustable passive phase shifter with comparable circuit size. The presented circuit is well suited to wireless adaptive antenna transceivers, operating in accordance with the 802.11a, high-performance radio local-area-network and high-speed wireless-access-network type-a standard.
Oxidative Nitration of Alkenes with <i>tert</i>‐Butyl Nitrite and Oxygen (2011)

Abstract A method for the oxidative nitration of alkenes using a combination of tert ‐butyl nitrite and molecular oxygen to give β‐nitro alcohols and their nitrate derivatives has been developed. The present reaction provides a practical method for the synthesis of nitro compounds because of the mild reaction conditions, the use of inexpensive reagents and a simple experimental procedure.
AutoCITE (2005)

These results demonstrate that AutoCITE training with greatly reduced supervision from a therapist is as effective as standard one-on-one CI therapy.
miR-124 and miR-203 are epigenetically silenced tumor-suppressive microRNAs in hepatocellular carcinoma (2009)

MicroRNAs (miRNAs) are a class of small non-coding RNAs that, in general, negatively regulate gene expression. They have been identified in various tumor types, showing that different sets of miRNAs are usually deregulated in different cancers. Some miRNA genes harboring CpG islands undergo methylation-mediated silencing, a characteristic of many tumor suppressor genes. To identify such miRNAs in hepatocellular carcinoma (HCC), we first examined the methylation status of 43 loci containing CpG islands around 39 mature miRNA genes in a panel of HCC cell lines and non-cancerous liver tissues as controls. Among 11 miRNA genes frequently methylated in HCC cell lines but not in non-cancerous liver tissues, three miRNA genes, i.e. miR-124, miR-203 and miR-375, were selected as silenced miRNAs through CpG-island methylation by comparing methylation and expression status and evaluating restored expression after treatment with 5-aza-2'-deoxycytidine. In primary tumors of HCC with paired non-tumorous liver tissues, only miR-124 and miR-203 showed frequent tumor-specific methylation, and their expression status was inversely correlated with methylation status. Ectopic expression of miR-124 or miR-203 in HCC cells lacking their expression inhibited cell growth, with direct downregulation of possible targets, cyclin-dependent kinase 6 (CDK6), vimentin (VIM), SET and MYND domain containing 3 (SMYD3) and IQ motif containing GTPase activating protein 1 (IQGAP1) or ATP-binding cassette, subfamily E, member 1 (ABCE1), respectively. Our results suggest that miR-124 and miR-203 are novel tumor-suppressive miRNAs for HCC epigenetically silenced and activating multiple targets during hepatocarcinogenesis.
NCMIR methods for 3D EM: a new protocol for preparation of biological specimens for serial block face scanning electron microscopy (2010)

Note: This protocol was designed to enhance signal for backscatter electron imaging of epoxy embedded mammalian tissue at low accelerating voltages (1-3 keV). However, it can easily be adapted for use with tissues from other species, tissue culture cells, plants and microbial cells by adjusting the buffer strength and the duration of relevant steps. This combinatorial heavy metal staining protocol employs a battery of contrasting steps after primary aldehyde fixation including: ferrocyanide reduced osmium tetroxide postfixation, thiocarbohydrazide-osmium liganding (OTO) and subsequent uranyl acetate and en bloc lead aspartate staining. Calcium chloride is included in a number of steps to enhance membrane preservation and staining. This protocol was designed primarily to emphasize the contrast of membranes. Many other contrasting agents may be included to increase staining of other cellular and extracellular constituents.
DNA Triple Helix Formation at Oligopurine Sites Containing Multiple Contiguous Pyrimidines (1997)

We have used DNase I footprinting to assess the formation of triple helices at 15mer oligopurine target sites which are interrupted by several (up to four) adjacent central pyrimidine residues. Third strand oligonucleotides were designed to generate complexes containing central (X.TA)nor (X.CG)n triplets (X = each base in turn) surrounded by C+.GC and T.AT triplets. It has previously been shown that G.TA and T.CG are the most stable triplets for recognition of single TA and CG interruptions. We show that these triplets are the most useful for recognizing consecutive pyrimidine interruptions and find that addition of each pyrimidine residue leads to a 30-fold decrease in third strand affinity. The addition of 10 microM naphthylquinoline triplex-binding ligand stabilizes each complex so that all the oligonucleotides produce footprints at similar concentrations (0.3 microM). Targets containing two pyrimidines are only bound by oligonucleotides generating (G.TA)2 and (T.CG)2 with a further 30-fold decrease in affinity. (G.TA)2 is slightly more stable than (T.CG)2. In the presence of the triplex-binding ligand the order of stability is (G.TA)2 > (C.TA)2 > (T.TA)2 > (A.TA)2 and (T.CG)2 > (C.CG)2 > (G.CG)2 = (A.CG)2. No oligonucleotide footprints are generated at target sites containing three consecutive pyrimidines, though addition of 10 microM triplex-binding ligand produces stable complexes with oligonucleotides generating (G.TA)3, (T.CG)3 and (C.CG)3, with a further 30-fold reduction in affinity. No footprints are generated at targets containing four Ts, though the ligand induces a weak interaction with the oligonucleotide generating (T.CG)4.
Ultra long high resolution beam by multi-zone rotationally symmetrical complex pupil filter (2007)

An ultra long high resolution beam with extension of depth of focus (DoF) in the axial direction as well as high resolution in the transverse direction has been demonstrated by a seven-zone rotationally symmetrical complex pupil filter imposed at the aperture of a focusing lens. Both amplitude and phase of the transmitted light are modulated in different zones. The scalar diffraction theory is used to optimize the zone parameters. Simulation results show that extended DoF of the beam is increased by 16 times while the spot size at the beam waist is reduced to 0.7 times.
The Effects of Adhesive Thickness on Polymerization Contraction Stress of Composite (2000)

A layer of an unfilled adhesive resin placed between the tooth and composite restoration has been shown to absorb some of the stress generated in the composite during polymerization and to reduce interfacial leakage. The objectives of this study were to measure the change in polymerization contraction stress of bonded composite as the thickness of the resin adhesive was systematically varied, and to correlate the effects of the adhesive thickness and reduced stress on marginal leakage in class V cavities. The maximum contraction force of composite (Herculite XRV) was measured in a tensilometer as the thickness of the adhesive bonding agent (Scotchbond MP) was varied from 20 to 300 microm. Composite was placed in Class V cavities prepared on the labial surfaces of bovine teeth to which different thicknesses of adhesive had been applied by layering, and a marginal leakage test was performed by means of staining with silver nitrate. Contraction stress decreased significantly as the adhesive thickness was increased. This result was supported by a theoretical examination of the data. In class V cavities, additional adhesive layering in the marginal area reduced the overall degree of microleakage. The contraction stress generated during the placement of a composite restoration contributes significantly to early marginal leakage, and this stress was significantly absorbed and relieved by the application of an increasing thickness of low-stiffness adhesive.
Crystallization Behavior of Poly(ethylene oxide) in Vertically Aligned Carbon Nanotube Array (2018)

We investigate the effect of the presence of vertically aligned multiwalled carbon nanotubes (CNTs) on the orientation of poly(ethylene oxide) (PEO) lamellae and PEO crystallinity. The high alignment of carbon nanotubes acting as templates probably governs the orientation of PEO lamellae. This templating effect might result in the lamella planes of PEO crystals oriented along a direction parallel to the long axis of the nanotubes. The presence of aligned carbon nanotubes also gives rise to the decreases in PEO crystallinity, crystallization temperature, and melting temperature due to the perturbation of carbon nanotubes to the crystallization of PEO. These effects have significant implications for controlling the orientation of PEO lamellae and decreasing the crystallinity of PEO and thickness of PEO lamellae, which have significant impacts on ion transport in PEO/CNT composite and the capacitive performance of PEO/CNT composite. Both the decreased PEO crystallinity and the orientation of PEO lamellae along the long axes of vertically aligned CNTs give rise to the decrease in the charge transfer resistance, which is associated with the improvements in the ion transport and capacitive performance of PEO/CNT composite.
Perception of Chemotherapy Side Effects Cancer versus Noncancer Patients (1999)

Noncancer, chemotherapy-naive patients perceived most chemotherapy-associated side effects as having greater impact on the quality of life than did cancer patients who had received chemotherapy. These findings can be used to direct patient education, education of the public, specific materials concerning cancer chemotherapy. The expertise of various members of the healthcare team can maximize the patient's comprehension of the adverse effects of the treatment options. The physician's knowledge of the overall treatment plan can assist in patient understanding; oncology pharmacists nurses are in a unique position to educate patients their families regarding potential chemotherapy side effects.
The Spinal Tap: A New Look at an Old Test (1986)

The spinal tap, or lumbar puncture, has indisputable value; opinions differ, however, on the amount of that value. The procedure has variable utility depending on the clinical indications and the results of tests on the cerebrospinal fluid. Its greatest value is in the evaluation of infectious or malignant meningitis; for most other diseases, it provides additional, but not essential, information. Because of the potential risk of the spinal tap, decisions about when to do the procedure must be made carefully. A probability analysis is provided to elucidate the usefulness of data from cerebrospinal fluid tests.
Plasmid-associated bacteriocin production by a strain of Carnobacterium piscicola from meat (1990)

Carnobacterium piscicola LV17 isolated from vacuum-packed meat produces bacteriocin(s) that is active against closely related lactic acid bacteria, Enterococcus spp., and a strain of Listeria monocytogenes but not against gram-negative bacteria. The bacteriocin has a bactericidal mode of action, is heat resistant, and is stable over a wide range of pH but is inactivated by proteolytic enzymes. Sensitive and resistant cells were shown to adsorb the bacteriocin, but cell death depended on contact of the bacteriocin with the cell membrane. Bacteriocin production is detected early in the growth cycle of the organism in APT broth, but it is not produced in APT broth adjusted to pH 5.5. Bacteriocin production and resistance to the bacteriocin produced are associated with two plasmids of 40 and 49 megadaltons. The possibility that two bacteriocins are produced is indicated because the inhibitory substances of the mutant strains containing either the 40- or 49-megadalton plasmids have different antimicrobial spectra.
Non-Hertzian Approach to Analyzing Mechanical Properties of Endothelial Cells Probed by Atomic Force Microscopy (2005)

Detailed measurements of cell material properties are required for understanding how cells respond to their mechanical environment. Atomic force microscopy (AFM) is an increasingly popular measurement technique that uniquely combines subcellular mechanical testing with high-resolution imaging. However, the standard method of analyzing AFM indentation data is based on a simplified "Hertz" theory that requires unrealistic assumptions about cell indentation experiments. The objective of this study was to utilize an alternative "pointwise modulus" approach, that relaxes several of these assumptions, to examine subcellular mechanics of cultured human aortic endothelial cells (HAECs). Data from indentations in 2- to 5-microm square regions of cytoplasm reveal at least two mechanically distinct populations of cellular material. Indentations colocalized with prominent linear structures in AFM images exhibited depth-dependent variation of the apparent pointwise elastic modulus that was not observed at adjacent locations devoid of such structures. The average pointwise modulus at an arbitrary indentation depth of 200 nm was 5.6+/-3.5 kPa and 1.5+/-0.76 kPa (mean+/-SD, n=7) for these two material populations, respectively. The linear structures in AFM images were identified by fluorescence microscopy as bundles of f-actin, or stress fibers. After treatment with 4 microM cytochalasin B, HAECs behaved like a homogeneous linear elastic material with an apparent modulus of 0.89+/-0.46 kPa. These findings reveal complex mechanical behavior specifically associated with actin stress fibers that is not accurately described using the standard Hertz analysis, and may impact how HAECs interact with their mechanical environment.
Global Least-Squares Analysis of Large, Correlated Spectral Data Sets:  Application to Component-Resolved FT-PGSE NMR Spectroscopy (1996)

A new data processing mode for Fourier Transform Pulsed-Gradient Spin-Echo (FT-PGSE) data sets is described. Unlike conventional analysis methods, it uses all of the significant spectral information of a data set of typically 16 or 32 different magnetic field gradient settings for 10−1000 significant frequency channels out of a 1−16K FT-PGSE data set. The procedure is based on a global least-squares minimization approach at two levels: an upper level that optimizes the actual global self-diffusion coefficient data and a lower one that optimizes the amplitude(s) of the component(s) for a particular frequency channel. This approach relies on the intrinsic property of FT-PGSE data sets in that the whole bandshape of a particular component attenuates by exactly the same relative amount upon incrementing the field gradient pulse parameters (Stilbs, P. Anal. Chem. 1981, 53, 2135 which was also shown to provide a pathway for separating the spin-echo bandshapes of the constituents of multicomponent systems. As a consequence of the coupled, global minimization approach of the method, the signal-to-noise ratio (S/N) of the FT-PGSE experiment is enhanced by typically a factor of 10 or more, since all of the available spectral information is utilized (effectively, a few 100 frequency channels/peak are combined). The present (global) optimization procedure (named CORE-NMR, COmponent-REsolved NMR spectroscopy) fundamentally differs from the diffusion-ordered spectroscopy procedure(s) introduced by Johnson et al., but the two approaches can be regarded as complementary. CORE-NMR is expected to find particular use in current studies on aggregation and binding in polymer and surfactant solutions, solving evaluation problems originating from the poor S/N, overlapping bandshapes, and high dynamic range with regard to relative constituent spectral intensities. Typically these difficulties are all present at the same time in such studies. CORE-NMR is equally well applicable to electrophoretic FT-NMR, where the signals of a particular component also vary coherently with an experimental parameter (the electrophoretic current) with regard to intensity and phase. As outlined, the generic CORE approach is of course also applicable to any other type of spectroscopic data, where individual intensities of separated or overlapping component spectral bandshapes decay/evolve in a similarly correlated manner as in, e.g., FT-PGSE NMR.
Insight into the cyclization of 6-octen-1-als with rhodium (I) complex. (1989)

Cyclization of 6-octen-1-als with Rh(I) (Wilkinson) complex was examined. In all cases with methyl substituents at the C2- or C3-position, a mixture of cis- and trans-cyclohexanol derivatives was obtained, but 7-methyl-6-octen-1-al without any substituents instead underwent a decarbonylation reaction. 4-Oxa-analogues also afforded a similar result. In Rh(I)-catalyzed cyclization, the formation of cis-cyclohexanols is in remarkable contrast to Lewis acid-catalyzed cyclization to afford the trans products.