2130 ROBUST ERG ONCOPROTEIN EXPRESSION IN PROSTATE CANCER: POTENTIAL FOR ERG BASED STRATIFICATION (2010)

You have accessJournal of UrologyProstate Cancer: Markers II1 Apr 20102130 ROBUST ERG ONCOPROTEIN EXPRESSION IN PROSTATE CANCER: POTENTIAL FOR ERG BASED STRATIFICATION Bungo Furusato, Shyh-Han Tan, Denise Young, Albert Dobi, Chen Sun, Ahmed Mohamed, Rajesh Thangapazham, Yongmei Chen, Gary McMaster, Taduru Sreenath, Gyorgy Petrovics, David G. McLeod, Shiv Srivastava, and Isabell Sesterhenn Bungo FurusatoBungo Furusato Rockville, MD More articles by this author , Shyh-Han TanShyh-Han Tan Rockville, MD More articles by this author , Denise YoungDenise Young Washington, DC More articles by this author , Albert DobiAlbert Dobi Rockville, MD More articles by this author , Chen SunChen Sun Rockville, MD More articles by this author , Ahmed MohamedAhmed Mohamed Rockville, MD More articles by this author , Rajesh ThangapazhamRajesh Thangapazham Rockville, MD More articles by this author , Yongmei ChenYongmei Chen Rockville, MD More articles by this author , Gary McMasterGary McMaster Fremont, CA More articles by this author , Taduru SreenathTaduru Sreenath Rockville, MD More articles by this author , Gyorgy PetrovicsGyorgy Petrovics Rockville, MD More articles by this author , David G. McLeodDavid G. McLeod Washington, DC More articles by this author , Shiv SrivastavaShiv Srivastava Rockville, MD More articles by this author , and Isabell SesterhennIsabell Sesterhenn Washington, DC More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2010.02.2218AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Gene fusions prevalent in prostate cancer lead to the elevated expression of the ERG proto-oncogene. Despite extensive efforts to evaluate gene fusions at the genomic and transcript levels, protein products of gene fusions remain to be defined in prostate tumors. The objective of this study was to obtain a global view of the ERG protein expression in the context of multifocal prostate cancer, using an anti-ERG monoclonal antibody (ERG-MAb) developed by our group. METHODS ERG-MAb was rigorously characterized for its specificity using VCaP and LNCaP prostate cancer cells lines and ectopic expression of ERG in HEK 293 cells. One representative whole mount cross section containing one to four tumor foci was selected from 132 radical prostatectomy (RP) specimens. Chi square test, Fisher exact test, analysis of variances (ANOVA) or Kruskal-Wallis test were used to examine the association of ERG positive tumor cells with clinico-pathological features. RESULTS Through exhaustive evaluations of 132 whole-mount prostates (227 tumor foci and over 200,000 benign glands), we demonstrate over 99.9 % specificity of the anti-ERG in detecting prostate carcinoma with positive frequencies of 50% (114 out of 227 specimens) and 61% (80 out of 132 patients). Fifty three percent of the sections (37 out of 71) with single focus tumors were ERG+, whereas 69% of sections (42 of 61) with multi-focal tumors were ERG+. Unequivocal relationship of ERG+ prostatic intraepithelial neoplasia (PIN) with ERG+ carcinoma (80 out of 80 patients) suggested for clonal progression of ERG+ pre-invasive lesions. CONCLUSIONS Homogeneous and strong ERG oncoprotein expression (99.9% specificity) as revealed in individual tumors of whole mount RP specimens may provide a potential breakthrough in ERG based stratification of prostate cancers. The concordance between all cases with focally ERG positive PIN and carcinoma strongly supports the biological role of ERG in clonal progression. © 2010 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 183Issue 4SApril 2010Page: e827-e828 Advertisement Copyright & Permissions© 2010 by American Urological Association Education and Research, Inc.MetricsAuthor Information Bungo Furusato Rockville, MD More articles by this author Shyh-Han Tan Rockville, MD More articles by this author Denise Young Washington, DC More articles by this author Albert Dobi Rockville, MD More articles by this author Chen Sun Rockville, MD More articles by this author Ahmed Mohamed Rockville, MD More articles by this author Rajesh Thangapazham Rockville, MD More articles by this author Yongmei Chen Rockville, MD More articles by this author Gary McMaster Fremont, CA More articles by this author Taduru Sreenath Rockville, MD More articles by this author Gyorgy Petrovics Rockville, MD More articles by this author David G. McLeod Washington, DC More articles by this author Shiv Srivastava Rockville, MD More articles by this author Isabell Sesterhenn Washington, DC More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...