gebhart/patrepeval huggingface dataset:
On the mechanism of wing size determination in fly development (2007)

A fundamental and unresolved problem in animal development is the question of how a growing tissue knows when it has achieved its correct final size. A widely held view suggests that this process is controlled by morphogen gradients, which adapt to tissue size and become flatter as tissue grows, leading eventually to growth arrest. Here, we present evidence that the decapentaplegic (Dpp) morphogen distribution in the developing Drosophila wing imaginal disk does not adapt to disk size. We measure the distribution of a functional Dpp-GFP transgene and the Dpp signal transduced by phospho-Mad and show that the characteristic length scale of the Dpp profile remains approximately constant during growth. This finding suggests an alternative scenario of size determination, where disk size is determined relative to the fixed morphogen distribution by a certain threshold level of morphogen required for growth. We propose that when disk boundary reaches the threshold the arrest of cell proliferation throughout the disk is induced by mechanical stress in the tissue. Mechanical stress is expected to arise from the nonuniformity of morphogen distribution that drives growth. This stress, through a negative feedback on growth, can compensate for the nonuniformity of morphogen, achieving uniform growth with the rate that vanishes when disk boundary reaches the threshold. The mechanism is demonstrated through computer simulations of a tissue growth model that identifies the key assumptions and testable predictions. This analysis provides an alternative hypothesis for the size determination process. Novel experimental approaches will be needed to test this model.
Cross-talk in the A1-ATPase from Methanosarcina mazei Gö1 Due to Nucleotide Binding (2002)

Changes in the A(3)B(3)CDF-complex of the Methanosarcina mazei Gö1 A(1)-ATPase in response to ligand binding have been studied by small-angle x-ray scattering, protease digestion, fluorescence spectroscopy, matrix-assisted laser desorption ionization time-of-flight mass spectrometry, and CuCl(2)-induced disulfide formation. The value of the radius of gyration, R(g), increases slightly when MgATP, MgADP, or MgADP + P(i) (but not MgAMP-PNP) is present. The nucleotide-binding subunits A and B were reacted with N-4[4-[7-(dimethylamino)-4-methyl]coumarin-3-yl]maleimide, and spectral shifts and changes in fluorescence intensity were detected upon addition of MgAMP-PNP, MgATP, MgADP + P(i), or MgADP. Trypsin treatment of A(1) resulted in cleavage of the stalk subunits C and F, which was rapid in the presence of MgAMP-PNP but slow when MgATP or MgADP were added to the enzyme. When A(1) was supplemented with CuCl(2) a clear nucleotide dependence of an A-A-D cross-linking product was generated in the presence of MgADP and MgATP but not when MgAMP-PNP or MgADP + P(i) was added. The site of cross-link formation was located in the region of the N and C termini of subunit D. The data suggest that the stalk subunits C, D, and F in A(1) undergo conformational changes during ATP hydrolysis.
μTrench phase-change memory cell engineering and optimization (2005)

Phase-change memory (PCM) cell is the most promising technology as post-flash nonvolatile memory (NVM). Among the different proposed cell structures, /spl mu/trench allows to simultaneously achieve low programming currents, small cell size, easy tunability and good dimensional control. Aim of this paper is to investigate the optimization and fine tuning capability of this cell architecture. Based on theoretical analysis and experimental results, the dependence of the PCM electrical parameters on the cell structure and on the geometric dimensions is assessed. An optimized /spl mu/trench cell with a programming current of 450 /spl mu/A at 1.5 V and a set resistance of 5 k/spl Omega/ is finally presented.
Synthesis of network reduced graphene oxide in polystyrene matrix by a two-step reduction method for superior conductivity of the composite (2012)

Polymer/graphene composites have attracted much attention due to their unique organic–inorganic hybrid structure and exceptional properties. In this paper, we report the synthesis of polystyrene/reduced graphene oxide (PS/r-GO) composites by a two-step in situ reduction technique, which consists of a hydrazine hydrate reduction and a subsequent thermal reduction at 200 °C for 12 h. The structure and micromorphology of PS/r-GO composites were characterized by scanning electron microscopy, transmission electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, Raman spectroscopy, X-ray photoelectron spectroscopy and thermogravimetric analysis. The results show that the GO can be efficiently reduced by the two-step in situ reduction method, and the r-GO sheets are well dispersed and ultimately form a continuous network structure in the polymer matrix. PS/r-GO composite films (5 wt% GO) are prepared by the hot press molding method, possessing a conductivity as high as 22.68 S m−1. The superior conductivity arises from the high reduction degree of GO and its high dispersion and the formation of a network structure in the polymer matrix. These polymer/r-GO composites are expected to be applied in multiple electric devices. The techniques for preparing polymer/r-GO composite films could be further extended to other similar systems.
Single intrathecal administration of the transcription factor decoy AYX1 prevents acute and chronic pain after incisional, inflammatory, or neuropathic injury (2013)

The persistence of pain after surgery increases the recovery interval from surgery to a normal quality of life. AYX1 is a DNA-decoy drug candidate designed to prevent post-surgical pain following a single intrathecal injection. Tissue injury causes a transient activation of the transcription factor EGR1 in the dorsal root ganglia-dorsal horn network, which then triggers changes in gene expression that induce neuronal hypersensitivity. AYX1 is a potent, specific inhibitor of EGR1 activity that mimics the genomic EGR1-binding sequence. Administered in the peri-operative period, AYX1 dose dependently prevents mechanical hypersensitivity in models of acute incisional (plantar), inflammatory (CFA), and chronic neuropathic pain (SNI) in rats. Furthermore, in a knee surgery model evaluating functional measures of postoperative pain, AYX1 improved weight-bearing incapacitance and spontaneous rearing compared to control. These data illustrate the potential clinical therapeutic benefits of AYX1 for preventing the transition of acute to chronic post-surgical pain.
An online cost sensitive decision-making method in crowdsourcing systems (2013)

Crowdsourcing has created a variety of opportunities for many challenging problems by leveraging human intelligence. For example, applications such as image tagging, natural language processing, and semantic-based information retrieval can exploit crowd-based human computation to supplement existing computational algorithms. Naturally, human workers in crowdsourcing solve problems based on their knowledge, experience, and perception. It is therefore not clear which problems can be better solved by crowdsourcing than solving solely using traditional machine-based methods. Therefore, a cost sensitive quantitative analysis method is needed.
Air-Breathing System Using Reed Valve for Pulse Detonation Microwave Rocket (2016)

An air-breathing system using reed valves was developed for Microwave Rocket. No stopper plate was used to avoid interference between the reed valve tip and high-power microwave beams in the thruster tube. Titanium alloy was used for the reed petal because of its high strength. The petal's width and thickness were designed to be tapered to prevent the petal from yielding, and its fundamental frequency, opening area, and the maximum stress on the petal were computed using static analysis. Estimated fundamental frequency agreed well with the measurement, though the opening area and the maximum stress were underestimated because of the inertial force. The experiment was conducted using a high-power microwave generator (gyrotron) to evaluate the air-breathing performance. In the experiment, no plastic deformation was observed on the reed petal and the partial filling rate was deduced as 0.32-0.44 for two reed valve stages (16 reed valves).
Lateral optical confinement of channeled-substrate-planar lasers with GaAs/AlGaAs substrates (1988)

A physical explanation of the lateral guiding mechanism in channeled-substrate-planar (CSP) lasers based on the amount of wavefront tilt of the transverse field outside the channel region is presented. Because of this inherent wavefront tilt, all CSP lasers will have a very slight asymmetry in their transverse far-field pattern. The nature of the guiding mechanism does not require light absorption by the substrate. Design curves showing the complex lateral effective index step as a function of n-clad thickness with the active layer as a parameter are also presented. Depending on the specific layer compositions and thicknesses, the CSP guiding mechanism can provide a positive lateral index step for substrates with mole fractions of AlAs ranging from 0 to higher than 0.2.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
An immunohistochemical study of TIMP-3 expression in oesophageal squamous cell carcinoma (2004)

Tissue inhibitor of metalloproteinase-3 (TIMP-3) inhibits the activity of matrix metalloproteinase, which may play an important role in carcinoma invasion and metastasis. We have investigated the relationship between TIMP-3 reduction and clinicopathological factors in oesophageal squamous cell carcinoma (ESCC). We examined tissue specimens that had been removed from 90 patients with thoracic oesophageal cancer who had undergone surgery between 1983 and 2001. Immunohistochemical staining was performed by the standard streptavidin-biotin method. Immunostaining of TIMP-3 was seen in the cytoplasm of cancer cells and normal oesophageal epithelial cells, particularly in cells located in shallow areas of the tumour. TIMP-3 preserved (+), moderate (+/-), and reduced (-) cases accounted for 30, 27, and 33 of the 90 patients, respectively (33, 30, 37%). Significant correlations were observed between TIMP-3 expression and depth of tumour invasion (P=0.001), number of lymph node metastases (P=0.003), infiltrative growth pattern (P=0.003), and disease stage (P=0.005). The survival rates of patients with TIMP-3 (-) cancer were significantly lower than those of patients with TIMP-3 (+) and TIMP-3 (+/-) cancer (P=0.0003). The mean 5-year survival rates of patients with TIMP-3 (+), (+/-), and (-) were 50, 58, and 21%, respectively. In conclusion, decreased expression of TIMP-3 protein correlates with invasive activity and metastasis. This makes the prognosis for patients with cancer that has lost TIMP-3 significantly less favourable than that for patients with cancer that has maintained TIMP-3.
Photorefractive composites with high-band-gap second-order nonlinear optical chromophores (1995)

A series of photorefractive composite materials has been developed in which an inert polymer has been doped with second-order nonlinear optical chromophores, charge transporting agents, and photocharge generation sensitizers. The composites show high photoconductivity, large photorefractive responses, and optical transparency at many wavelengths. Photorefractivity has been demonstrated at wavelengths of 633, 514.5, and 488 nm, with net two-beam coupling gains.
High-Frequency Gate-All-Around Vertical InAs Nanowire MOSFETs on Si Substrates (2014)

We demonstrate a vertical InAs nanowire MOSFET integrated on Si substrate with an extrinsic peak cut-off frequency of 103 GHz and a maximum oscillation frequency of 155 GHz. The transistor has a transconductance of 730 mS/mm and is based on arrays of nanowires with gate-all-around and high-κ gate dielectric. Furthermore, small-signal modeling shows a ~80% reduction of the total parasitic gate capacitance when the metal pad overlap in the transistors is reduced through additional patterning.
Efficient MgBr2.OEt2 - catalyzed Knoevenagel condensation (2006)

An efficient room-temperature procedure was developed for Knoevenagel condensation of various aldehydes with malononitrile and ethyl cyanoacetate in the presence of triethylamine and catalytic amounts of magnesium bromide diethyl etherate.
Angiogenesis Mediated by Toll-Like Receptor 4 in Ischemic Neural Tissue (2012)

Our results suggest that the release of high-mobility group box-1 in ischemic neural tissue initiates TLR4-dependent responses that contribute to neovascularization. These findings represented a previously unrecognized effect of TLR4 on angiogenesis in association with the activation of glia in ischemic neural tissue.
Día del periodista: de premios y antipremios (2019)

A partir de la entrega de los Premios Nacionales de Periodismo, tradicion que se ha mantenido desde que se promulgo el Dia Nacional del Periodista, la articulista nos comenta como se han tergiversado los reconocimientos, a lo largo de estos veinte anos del proceso, por razones politicas e ideologicas. Ahora se premia la fidelidad al regimen, la mentira y la manipulacion informativa.
Elastic property measurement using Rayleigh-Lamb waves (1995)

Abstract A nondestructive technique is described for the measurement of elastic constants of isotropic plates using ultrasonic Rayleigh-Lamb waves. The experimental method employs continuous harmonic waves and a pair of variable-angle contact transducers in pitch-catch mode. The phase velocity of the R-L waves at a particular frequency is determined from the phase shift over a measured path length. This simple experimental technique can measure phase velocity over the range 1–10 mm/μs with an error of less than 0.5% over a frequency range of 50 kHz-2 MHz. Individual symmetric and antisymmetric modes can be generated through the selection of transducer angle and frequency. Young's modulus and Poisson's ratio for the material are calculated from measurements of frequency and phase velocity by a nonlinear least squares solution to the dispersion equations. The sensitivity of the nonlinear least squares function to the measurement region of the dispersion curve is investigated. It was found that estimations of material properties are more accurate and less sensitive to small experimental errors when only selected frequencies and R-L modes are used in the least squares calculation. This technique is demonstrated with several isotropic materials and with both thick (6 mm) and thin (0.8 mm) plates. Values for elastic constants determined by the contact transducer Lamb wave technique compare favorably with values measured using the pulse-echo-overlap method. The uncertainty in measurements of Young's modulus and Poisson's ratio was less than 1% and 2%, respectively. The technique has advantages over more traditional methods for measuring elastic properties when it is desirable to use wavelengths greater than the plate thickness, when properties may vary with frequency, or when it is necessary to measure in-plane elastic properties of thin plate structures.
Kinetics of esmolol, an ultra-short-acting beta blocker, and of its major metabolite (1983)

Esmolol is an ultra-short-acting beta blocker. Its kinetics was studied in eight healthy subjects after continuous intravenous infusion of 400 micrograms/kg/min over 2 hr. The concentrations of esmolol and its major metabolite, 3-[4-(2-hydroxy-3-[isopropylamino]propoxy)phenyl]propionic acid, in blood and urine were determined by gas chromatographic-mass spectrometric assay and HPLC. The distribution and elimination t1/2s of esmolol averaged 2.03 and 9.19 min. The apparent volume of distribution of esmolol averaged 3.43 l/kg and was four times the volume of the central compartment. The total clearance of esmolol averaged 285 ml/min/kg, indicating that nonhepatic routes play a predominant role in its clearance. The t1/2s of formation and elimination of the metabolite averaged 2.82 min and 3.72 hr. The ratio of the metabolite formation and elimination rate constants of the parent drug (kf/k10) averaged 0.829, suggesting that 82.9% of esmolol was converted to the metabolite (which is consistent with the urinary recovery of 71% of the dose as unconjugated metabolite). The volume of distribution and total clearance of the metabolite averaged 0.411 l/kg and 1.28 ml/min/kg. Esmolol was followed by a significant reduction of isoproterenol-induced increase in heart rate and systolic blood pressure at doses of 50, 150, and 400 micrograms/kg/min.(ABSTRACT TRUNCATED AT 250 WORDS)
Component-Based Face Recognition with 3D Morphable Models (2005)

We present a system for pose and illumination invariant face recognition that combines two recent advances in the computer vision field: 3D morphable models and component-based recognition. A 3D morphable model is used to compute 3D face models from three input images of each subject in the training database. The 3D models are rendered under varying pose and illumination conditions to build a large set of synthetic images. These images are then used for training a component-based face recognition system. The face recognition module is preceded by a fast hierarchical face detector resulting in a system that can detect and identify faces in video images at about 4 Hz. The system achieved a recognition rate of 88% on a database of 2000 real images of ten people, which is significantly better than a comparable global face recognition system. The results clearly show the potential of the combination of morphable models and component-based recognition towards pose and illumination invariant face recognition.
Mobility and handoff management in vehicular networks: a survey (2009)

Abstract Mobility management is one of the most challenging research issues for vehicular networks to support a variety of intelligent transportation system (ITS) applications. The traditional mobility management schemes for Internet and mobile ad hoc network (MANET) cannot meet the requirements of vehicular networks, and the performance degrades severely due to the unique characteristics of vehicular networks (e.g., high mobility). Therefore, mobility management solutions developed specifically for vehicular networks would be required. This paper presents a comprehensive survey on mobility management for vehicular networks. First, the requirements of mobility management for vehicular networks are identified. Then, classified based on two communication scenarios in vehicular networks, namely, vehicle‐to‐vehicle (V2V) and vehicle‐to‐infrastructure (V2I) communications, the existing mobility management schemes are reviewed. The differences between host‐based and network‐based mobility management are discussed. To this end, several open research issues in mobility management for vehicular networks are outlined. Copyright © 2009 John Wiley & Sons, Ltd.
mTOR's role in ageing: protein synthesis or autophagy? (2009)

The molecular and cellular mechanisms that regulate ageing are currently under scrutiny because ageing is linked to many human diseases. The nutrient sensing TOR pathway is emerging as a key regulator of ageing. TOR signaling is complex affecting several crucial cellular functions and two such functions, which show clear effects on ageing, are protein synthesis and autophagy. In this article we discuss the relative importance of both these processes in ageing, identify how TOR regulates translation and autophagy and speculate on links between the TOR signaling network and ageing pathways.
Boric acid reversibly inhibits the second step of pre‐mRNA splicing (2003)

Several approaches have been used to identify the factors involved in mRNA splicing. None of them, however, comprises a straightforward reversible method for inhibiting the second step of splicing using an external reagent other than a chelator. This investigation demonstrates that the addition of boric acid to an in vitro pre-mRNA splicing reaction causes a dose-dependent reversible inhibition effect on the second step of splicing. The mechanism of action does not involve chelation of several metal ions; hindrance of 3' splice-site; or binding to hSlu7. This study presents a novel method for specific reversible inhibition of the second step of pre-mRNA splicing.
Establishing glucose- and ABA-regulated transcription networks in <i>Arabidopsis</i> by microarray analysis and promoter classification using a Relevance Vector Machine (2006)

Establishing transcriptional regulatory networks by analysis of gene expression data and promoter sequences shows great promise. We developed a novel promoter classification method using a Relevance Vector Machine (RVM) and Bayesian statistical principles to identify discriminatory features in the promoter sequences of genes that can correctly classify transcriptional responses. The method was applied to microarray data obtained from Arabidopsis seedlings treated with glucose or abscisic acid (ABA). Of those genes showing >2.5-fold changes in expression level, approximately 70% were correctly predicted as being up- or down-regulated (under 10-fold cross-validation), based on the presence or absence of a small set of discriminative promoter motifs. Many of these motifs have known regulatory functions in sugar- and ABA-mediated gene expression. One promoter motif that was not known to be involved in glucose-responsive gene expression was identified as the strongest classifier of glucose-up-regulated gene expression. We show it confers glucose-responsive gene expression in conjunction with another promoter motif, thus validating the classification method. We were able to establish a detailed model of glucose and ABA transcriptional regulatory networks and their interactions, which will help us to understand the mechanisms linking metabolism with growth in Arabidopsis. This study shows that machine learning strategies coupled to Bayesian statistical methods hold significant promise for identifying functionally significant promoter sequences.