gebhart/patrepeval huggingface dataset:
The oncolytic effect of recombinant vesicular stomatitis virus is enhanced by expression of the fusion cytosine deaminase/uracil phosphoribosyltransferase suicide gene. (2003)

Vesicular stomatitis virus (VSV) has recently been demonstrated to exhibit significant oncolytic capabilities against a wide variety of tumor models in vitro and in vivo. To potentially enhance the oncolytic effect, we generated a novel recombinant VSV (rVSV) that expressed the fusion suicide gene Escherichia coli cytosine deaminase (CD)/uracil phosphoribosyltransferase (UPRT). rVSV encoding the CD/UPRT fusion gene (VSV-C:U) exhibited normal growth properties and generated high levels of biologically active CD/UPRT that could catalyze the modification of 5-fluorocytosine into chemotherapeutic 5-fluorouracil (5-FU), which exhibited considerable bystander effect. Intratumoral inoculation of VSV-C:U in the presence of the systemically administered prodrug 5-fluorocytosine produced statistically significant reductions in the malignant growth of syngeneic lymphoma (A20) or mammary carcinoma (TSA) in BALB/c mice compared with rVSV treatments or with control 5-FU alone. Aside from detecting prolonged therapeutic levels of 5-FU in VSV-C:U-treated animals harboring TSA tumors and enhancing bystander killing of tumor cells, we demonstrated marked activation of IFN-gamma-secreting cytotoxic T cells by enzyme-linked immunospot analysis that may have also facilitated tumor killing. In conclusion, the insertion of the fusion CD/UPRT suicide gene potentiates the oncolytic efficiency of VSV by generating a strong bystander effect and by contributing to the activation of the immune system against the tumor without detrimentally altering the kinetics of virus-mediated oncolysis and may be useful in the treatment of malignant disease.
Erythema Chronicum Migrans and Lyme Arthritis: Cryoimmunoglobulins and Clinical Activity of Skin and Joints (1977)

We report the presence of serum cryoimmunoglobulins in patients with attacks of a newly described epidemic arthritis--Lyme arthritis--and in some patients with a characteristic skin lesion--erythema chronicum migrans--that sometimes precedes the onset of the arthritis. Seven patients who had cryoimmunoglobulins at the time of the skin lesion have developed arthritis; four patients without them have not. The cryoglobulins in patients with the skin lesion consisted primarily of immunoglobulin M (IgM); those in patients with arthritis often included both IgM and IgG. These findings support the hypothesis that a common origin exists for the skin and joint lesions and suggest that circulating immune complexes may have a pathogenetic role in Lyme arthritis.
A Nuclear Receptor Corepressor–Dependent Pathway Mediates Suppression of Cytokine-Induced C-Reactive Protein Gene Expression by Liver X Receptor (2006)

C-reactive protein (CRP), the prototypical human acute phase protein, is an independent risk predictor of future cardiovascular events, both in healthy individuals and in patients with known cardiovascular disease. In addition, previous studies indicate that CRP might have direct proatherogenic properties. Ligand activation of the liver X receptor (LXR), a member of the nuclear hormone receptor superfamily, inhibits inflammatory gene expression in macrophages and attenuates the development of atherosclerosis in various animal models. We demonstrate herein that 2 synthetic LXR ligands, T0901317 and GW3965, inhibit interleukin-1beta/interleukin-6-induced CRP mRNA and protein expression in human hepatocytes. Knockdown of LXRalpha/beta by short interfering RNAs completely abolished the inhibitory effect of the LXR agonist T0901317 on cytokine-induced CRP gene transcription. Transient transfection experiments with 5'-deletion CRP promoter constructs identified a region from -125 to -256 relative to the initiation site that mediated the inhibitory effect of LXR ligands on CRP gene transcription. Depletion of the nuclear receptor corepressor by specific short interfering RNA increased cytokine-inducible CRP mRNA expression and promoter activity and reversed LXR ligand-mediated repression of CRP gene transcription. Chromatin immunoprecipitation assays indicated that nuclear receptor corepressor is present on the endogenous CRP promoter under basal conditions. Cytokine-induced clearance of nuclear receptor corepressor complexes was inhibited by LXR ligand treatment, maintaining the CRP gene in a repressed state. Finally, treatment of C57Bl6/J mice with LXR ligands attenuated lipopolysaccharide-induced mouse CRP and serum amyloid P component gene expression in the liver, whereas no effect was observed in LXRalphabeta knockout mice. Our observations identify a novel mechanism of inflammatory gene regulation by LXR ligands. Thus, inhibition of CRP expression by LXR agonists may provide a promising approach to impact initiation and progression of atherosclerosis.
Proton magnetic resonance spectroscopy in the brain: Report of AAPM MR Task Group #9 (2002)

AAPM Magnetic Resonance Task Group #9 on proton magnetic resonance spectroscopy (MRS) in the brain was formed to provide a reference document for acquiring and processing proton (1H) MRS acquired from brain tissue. MRS is becoming a common adjunct to magnetic resonance imaging (MRI), especially for the differential diagnosis of tumors in the brain. Even though MR imaging is an offshoot of MR spectroscopy, clinical medical physicists familiar with MRI may not be familiar with many of the common practical issues regarding MRS. Numerous research laboratories perform in vivo MRS on other magnetic nuclei, such as 31P, 13C, and 19F. However, most commercial MR scanners are generally only capable of spectroscopy using the signals from protons. Therefore this paper is of limited scope, giving an overview of technical issues that are important to clinical proton MRS, discussing some common clinical MRS problems, and suggesting how they might be resolved. Some fundamental issues covered in this paper are common to many forms of magnetic resonance spectroscopy and are written as an introduction for the reader to these methods. These topics include shimming, eddy currents, spatial localization, solvent saturation, and post-processing methods. The document also provides an extensive review of the literature to guide the practicing medical physicist to resources that may be useful for dealing with issues not covered in the current article.
A formal characterization of epsilon serializability (1995)

Epsilon serializability (ESR) is a generalization of classic serializability (SR). In this paper, we provide a precise characterization of ESR when queries that may view inconsistent data run concurrently with consistent update transactions. Our first goal is to understand the behavior of queries in the presence of conflicts and to show how ESR in fact is a generalization of SR. So, using the ACTA framework, we formally express the intertransaction conflicts that are recognized by ESR and through that define ESR, analogous to the manner in which conflict-based serializability is defined. Secondly, expressions are derived for the amount of inconsistency (in a data item) viewed by a query and its effects on the results of a query. These inconsistencies arise from concurrent updates allowed by ESR. Thirdly, in order to maintain the inconsistencies within bounds associated with each query, the expressions are used to determine the preconditions that operations have to satisfy. The results of a query, and the errors in it, depend on what a query does with the (possibly inconsistent) data viewed by it. One of the important byproducts of this work is the identification of different types of queries which lend themselves to an analysis of the effects of data inconsistency on the results of the query.
Rapid Antimicrobial Susceptibility Testing on Clinical Urine Samples by Video-Based Object Scattering Intensity Detection (2021)

To combat the ongoing public health threat of antibiotic-resistant infections, a technology that can quickly identify infecting bacterial pathogens and concurrently perform antimicrobial susceptibility testing (AST) in point-of-care settings is needed. Here, we develop a technology for point-of-care AST with a low-magnification solution scattering imaging system and a real-time video-based object scattering intensity detection method. The low magnification (1-2×) optics provides sufficient volume for direct imaging of bacteria in urine samples, avoiding the time-consuming process of culture-based bacterial isolation and enrichment. Scattering intensity from moving bacteria and particles in the sample is obtained by subtracting both spatial and temporal background from a short video. The time profile of scattering intensity is correlated with the bacterial growth rate and bacterial response to antibiotic exposure. Compared to the image-based bacterial tracking and counting method we previously developed, this simple image processing algorithm accommodates a wider range of bacterial concentrations, simplifies sample preparation, and greatly reduces the computational cost of signal processing. Furthermore, development of this simplified processing algorithm eases implementation of multiplexed detection and allows real-time signal readout, which are essential for point-of-care AST applications. To establish the method, 130 clinical urine samples were tested, and the results demonstrated an accuracy of ∼92% within 60-90 min for UTI diagnosis. Rapid AST of 55 positive clinical samples revealed 98% categorical agreement with both the clinical culture results and the on-site parallel AST validation results. This technology provides opportunities for prompt infection diagnosis and accurate antibiotic prescriptions in point-of-care settings.
Bivalent binding on cells varies between anti-CD20 antibodies and is dose-dependent (2020)

Based on their mechanism of action, two types of anti-CD20 antibodies are distinguished: Type I, which efficiently mediate complement-dependent cytotoxicity, and Type II, which instead are more efficient in inducing direct cell death. Several molecular characteristics of these antibodies have been suggested to underlie these different biological functions, one of these being the manner of binding to CD20 expressed on malignant B cells. However, the exact binding model on cells is unclear. In this study, the binding mechanism of the Type I therapeutic antibodies rituximab (RTX) and ofatumumab (OFA) and the Type II antibody obinutuzumab (OBI) were established by real-time interaction analysis on live cells. It was found that the degree of bivalent stabilization differed for the antibodies: OFA was stabilized the most, followed by RTX and then OBI, which had the least amount of bivalent stabilization. Bivalency inversely correlated with binding dynamics for the antibodies, with OBI displaying the most dynamic binding pattern, followed by RTX and OFA. For RTX and OBI, bivalency and binding dynamics were concentration dependent; at higher concentrations the interactions were more dynamic, whereas the percentage of antibodies that bound bivalent was less, resulting in concentration-dependent apparent affinities. This was barely noticeable for OFA, as almost all molecules bound bivalently at the tested concentrations. We conclude that the degree of bivalent binding positively correlates with the complement recruiting capacity of the investigated CD20 antibodies.
Sleep disorders, epilepsy, and autism (2004)

The purpose of this review article is to describe the clinical data linking autism with sleep and epilepsy and to discuss the impact of treating sleep disorders in children with autism either with or without coexisting epileptic seizures. Studies are presented to support the view that sleep is abnormal in individuals with autistic spectrum disorders. Epilepsy and sleep have reciprocal relationships, with sleep facilitating seizures and seizures adversely affecting sleep architecture. The hypothesis put forth is that identifying and treating sleep disorders, which are potentially caused by or contributed to by autism, may impact favorably on seizure control and on daytime behavior. The article concludes with some practical suggestions for the evaluation and treatment of sleep disorders in this population of children with autism.
Fast video transcoding architectures for networked multimedia applications (2002)

In various networked multimedia applications, it is often necessary to change the bit-rate and format of a pre-encoded bit-stream. This can be achieved using a cascaded pixel-domain transcoder, which fully decodes an incoming bit-stream and then re-encodes the decoded pictures with the desired bit-rate or format. However, the cascaded pixel-domain transcoder is computationally expensive. To reduce the computations, several fast architectures have been proposed in the literature. However, these fast transcoder architectures introduce new limitations and are not drift-free. In this paper, we propose new techniques to implement a fast cascaded pixel-domain transcoder. We further discuss the limitation and speed of the different transcoder architectures. We also discuss the methods for adding watermark or company logo, using the different transcoder architectures.
Activated N-ras contributes to the chemoresistance of human melanoma in severe combined immunodeficiency (SCID) mice by blocking apoptosis. (1997)

Activation of the N-ras gene by point mutations occurs in about 15 % of all human melanomas. Using recently established melanoma severe combined immunodeficiency-human mouse xenotransplantation models, here we further investigate the biological significance of these mutations. We demonstrate that activated N-ras significantly contributes to the chemoresistance of human melanoma both in vitro and in vivo by blocking apoptosis. Overexpression of wild-type N-ras had no such effects. With antisense oligonucleotides and farnesyltransferase inhibitors, tools capable of blocking Ras function on the therapeutic horizon, our observation that activated N-ras is not a bystander but a factor worth targeting to improve therapeutic outcome in melanoma gains additional importance.
Two regions of the<i>Escherichia cqli</i>16S ribosomal RNA are important for decoding stop signals in polypeptide chain termination (1993)

Two regions of the 16S rRNA, helix 34, and the aminoacyl site component of the decoding site at the base of helix 44, have been implicated in decoding of translational stop signals during the termination of protein synthesis. Antibiotics specific for these regions have been tested to see how they discriminate the decoding of UAA, UAG, and UGA by the two polypeptide chain release factors (RF-1 and RF-2). Spectinomycin, which interacts with helix 34, stimulated RF-1 dependent binding to the ribosome and termination. It also stimulated UGA dependent RF-2 termination at micromolar concentrations but inhibited UGA dependent RF-2 binding at higher concentrations. Alterations at position C1192 of helix 34, known to confer spectinomycin resistance, reduced the binding of f[3H]Met-tRNA to the peptidyl-tRNA site. They also impaired termination in vitro, with both factors and all three stop codons, although the effect was greater with RF-2 mediated reactions. These alterations had previously been shown to inhibit EF-G mediated translocation. As perturbations in helix 34 effect both termination and elongation reactions, these results indicate that helix 34 is close to the decoding site on the bacterial ribosome. Several antibiotics, hygromycin, neomycin and tetracycline, specific for the aminoacyl site, were shown to inhibit the binding and function of both RFs in termination with all three stop codons in vitro. These studies indicate that decoding of all stop signals is likely to occur at a similar site on the ribosome to the decoding of sense codons, the aminoacyl site, and are consistent with a location for helix 34 near this site.
Immunological Properties of Donkeys Milk: Its Potential Use in the Prevention of Atherosclerosis (2007)

Donkey's milk is the best substitute of human milk for its content in lactose, proteins, minerals, and omega-3 fatty acids. Here, we have evaluated the effects of colostrum and milk from donkeys (Martina Franca breed) on the function of human peripheral blood mononuclear cells (PBMCs) at different intervals from lactation. Colostrum induced more IgA responses, while milk induced predominantly more IgG responses. Both milk and colostrum induced expression of CD25 and CD69 on PBMCs. The ability to induce release of interleukins (IL) (IL-12, IL-1 beta and IL-10) and tumor necrosis factor-alpha was confined only to milk, while colostrum was devoid of this capacity. Finally, both colostrum and milk induced nitric oxide (NO) release from PBMCs but milk exhibited a greater capacity than colostrum in NO generation. Taken together, these immunological activities exerted by both colostrum and milk from donkeys may be useful in the treatment of human immune-related diseases. In particular, NO induction by donkey's milk may be very useful in the prevention of atherosclerosis, being a strong vasodilator and an effective antimicrobial agent since pathogens and/or their products may play a proatherogenic role.
Balancing Power Signature in Secure Systems (2008)

Abstract—Dual-rail code, return-to-spacer protocol and hazard-free logic is used to make power consumption of synchronous circuits independent from data processed. A new compact dual-rail flip-flop is designed, whose power consumption is also data-independent. A method for negative gate optimisation of dual-rail logic is described, which results in faster and smaller circuits. A tool for dual-rail circuit optimisation is developed. The tool is interfaced to the Cadence CAD system. Dual-rail and single-rail benchmarks are simulated and compared. I.
A Surgical Assistant Workstation (SAW) Application for Teleoperated Surgical Robot System (2009)

The Surgical Assistant Workstation (SAW) modular software framework provides integrated support for robotic devices, imaging sensors, and a visualization pipeline for rapid prototyping of telesurgical research systems. Subsystems of a telesurgical system, such as the master and slave robots and the visualization engine, form a distributed environment that requires an efficient inter-process communication (IPC) mechanism. This paper describes the extension of the component-based framework provided by the cisst libraries, on which the SAW depends, from a multi-threaded (local) to a multi-process (networked) environment. The current implementation uses the Internet Communication Engine (ICE) middleware package, but the design does not depend on ICE and can accomodate other middleware choices. A telesurgical robot system based on the da Vinci hardware platform is used as a test case, with a research application that requires coordination between the left and right robot arms (a bimanual knot tying task). Performance measurements on this platform indicate minimal overhead due to the networking.
Solution Growth and Electrodeposited CuInSe2Thin Films (1983)

Description de la croissance des couches par les deux techniques en vue de l'application aux dispositifs photovoltaiques
The Relationship between Total Cloud Lightning Behavior and Radar-Derived Thunderstorm Structure (2012)

Abstract Total lightning detection systems have been in development since the mid-1980s and deployed in several areas around the world. Previous studies on total lightning found intra- and intercloud lightning (IC) activity tends to fluctuate significantly during the lifetime of thunderstorms and have indicated that lightning jumps or rapid changes in lightning flash rates are closely linked to changes in the vertical integrated liquid (VIL) reading on the National Weather Service’s Weather Surveillance Radar-1988 Doppler (WSR-88D) systems. This study examines the total lightning and its relationship to WSR-88D signatures used operationally to determine thunderstorm severity to highlight the potential benefit of a combined forecast approach. Lightning and thunderstorm data from the Dallas–Fort Worth, Texas, and Tucson, Arizona, areas from 2006 to 2009, were used to relate total lightning behavior and radar interrogation techniques. The results indicate that lightning jumps can be classified into severe wind, hail, or mixed-type jumps based on the behavior of various radar-based parameters. In 25 of 34 hail-type jumps and in 18 of 20 wind-type jumps, a characteristic change in cloud-to-ground (CG) versus IC lightning flash rates occurred prior to the report of severe weather. For hail-type jumps, IC flash rates increased, while CG flash rates were steady or decreased. For wind-type jumps, CG flash rates increased, while IC flash rates either increased (12 of 18) or were steady or decreased (6 of 18). Although not every lightning jump resulted in a severe weather report, the characteristic behavior in flash rates adds information to radar-based approaches for nowcasting the severe weather type.
Mortality in Celiac Disease, Intestinal Inflammation, and Gluten Sensitivity (2009)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Supporting software engineering education with a local Web site (1996)

article Free Access Share on Supporting software engineering education with a local Web site Authors: V. E. Veraart Murdoch University, Australia Murdoch University, AustraliaView Profile , S. L. Wright Murdoch University, Australia Murdoch University, AustraliaView Profile Authors Info & Claims ACM SIGCSE BulletinVolume 28Issue 1March 1996 pp 275–279https://doi.org/10.1145/236462.236555Online:01 March 1996Publication History 6citation164DownloadsMetricsTotal Citations6Total Downloads164Last 12 Months0Last 6 weeks0 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
Thermal degradation of methyl and ethyl cyanoacrylate oligomers (1981)

Abstract Thermal degradation of methyl and ethyl cyanoacrylate polymers of number‐average molecular weights 370–1350 is shown to proceed by depolymerisation at temperatures in the range 140°–180°C. Under the conditions of the thermogravimetric analysis technique employed, weight loss kinetics appear to be governed by diffusion of monomer vapour through the liquid sample. A mathematical model of this process is postulated.
Factors affecting the impact properties of mineral filled polypropylene (1990)

The impact strength depends on both the size and the shape of filler and is also affected by micromorphology. Poor impact strength is correlated with a high capacity for nucleation of cristallisation.