gebhart/patrepeval huggingface dataset:
Structural remodelling during chronic atrial fibrillation: act of programmed cell survival (2001)

Atrial fibrillation is the most common cardiac arrhythmia with an overall prevalence of almost 1%. Increasing prevalence and associated risks such as stroke and mortality have increased the need for better and more reliable therapeutic treatment. This has stimulated research to elucidate the pathophysiological mechanisms underlying atrial fibrillation. Atrial fibrillation is primarily characterised by electrical remodelling and functional deterioration. Both phenomena are reversible but after prolonged duration of atrial fibrillation, a discrepancy occurs between rapid electrical remodelling and slow recovery of contractile function. Recent studies have indicated that morphological remodelling might underlie this incongruity. In experimental models of lone atrial fibrillation, the remodelling involves cellular changes that are reminiscent of dedifferentiation and are characterised by cellular volume increase, myolysis, glycogen accumulation, mitochondrial changes and chromatin redistribution. The absence of clear signs of degeneration in these models points towards cardiomyocyte adaptation or a mechanism of programmed cell survival. In patients with atrial fibrillation cardiomyocyte degeneration does occur along with dedifferentiation which might be the result of underlying cardiac pathologies or longer duration of atrial fibrillation. In this review we focus on structural remodelling during atrial fibrillation. The different aspects of histological and ultrastructural changes as well as their role in atrial dysfunction and cardiomyocyte survival are discussed. We briefly describe the underlying molecular remodelling. and possible mechanisms responsible for remodelling involving calcium overload and stretch are presented.
A Low-Voltage Low-Power CMOS Weak Inversion Log Domain Auto Gain Control Module (2007)

This paper presents a novel scheme for low voltage low power (LVLP) log domain auto gain control (AGC) system. The AGC principle is based on proportionally biasing a CMOS weak inversion log compressor with the input signal strength. Due to the log domain processing, this AGC achieves a large dynamic range reduction (DRR) under low voltage and low power. Simulated results at 1-V supply voltage for 0.18-mum CMOS process validate the principle. The total quiescent drain current of the AGC core is 0.3 muA. The AGC bandwidth is 1 KHz. It is suitable for LVLP bio-medical application such as electrocardiogram (ECG) signal adapting.
Gallium and indium imaging agents. 2. Complexes of sulfonated catechoylamide sequestering agents (1982)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTGallium and indium imaging agents. 2. Complexes of sulfonated catechoylamide sequestering agentsVincent L. Pecoraro, Geoffrey B. Wong, and Kenneth N. RaymondCite this: Inorg. Chem. 1982, 21, 6, 2209–2215Publication Date (Print):June 1, 1982Publication History Published online1 May 2002Published inissue 1 June 1982https://doi.org/10.1021/ic00136a016RIGHTS & PERMISSIONSArticle Views139Altmetric-Citations17LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (815 KB) Get e-Alerts Get e-Alerts
Studies of the cellulolytic system of the filamentous fungus <i>Trichoderma reesei</i> QM 9414. Substrate specificity and transfer activity of endoglucanase I (1990)

Endoglucanase I from the filamentous fungus Trichoderma reesei catalyses hydrolysis and glycosyl-transfer reactions of cello-oligosaccharides. Initial bond-cleaving frequencies determined with 1-3H-labelled cello-oligosaccharides proved to be substrate-concentration-dependent. Using chromophoric glycosides and analysing the reaction products by h.p.l.c., kinetic data are obtained and, as typical for an endo-type depolymerase, apparent hydrolytic parameters (kcat., kcat./Km) increase steadily as a function of the number of glucose residues. At high substrate concentrations, and for both free cellodextrins and their aromatic glycosides, complex patterns (transfer reactions) are, however, evident. In contrast with the corresponding lactosides and 1-thiocellobiosides, and in conflict with the expected specificity, aromatic 1-O-beta-cellobiosides are apparently hydrolysed at both scissile bonds, yielding the glucoside as one of the main reaction products. Its formation rate is clearly non-hyperbolically related to the substrate concentration and, since the rate of D-glucose formation is substantially lower, strong indications for dismutation reactions (self-transfer) are again obtained. Evidence for transfer reactions catalysed by endoglucanase I further results from experiments using different acceptor and donor substrates. A main transfer product accumulating in a digest containing a chromophoric 1-thioxyloside was isolated and its structure elucidated by proton n.m.r. spectrometry (500 MHz). The beta 1-4 configuration of the newly formed bond was proved.
Building the Timber Frame House: The Revival of a Forgotten Craft (1980)

For centuries, post-and-beam construction has proved to be one of the most durable building techniques. It is being enthusiastically revived today not only for its sturdiness but because it can be easily insulated, it is attractive, and it offers the builder the unique satisfaction of working with timbers. Building the Timber Frame House is the most comprehensive manual available on the technique. In it you will find a short history, of timber framing and a fully illustrated discussion of the different kinds of joinery, assembly of timbers, and raising of the frame. There are also detailed sections on present-day design and materials, house plans, site development, foundation laying, insulation, tools, and methods.
Relevance of regulatory T cell promotion of donor-specific tolerance in solid organ transplantation (2012)

Current clinical strategies to control the alloimmune response after transplantation do not fully prevent induction of the immunological processes which lead to acute and chronic immune-mediated graft rejection, and as such the survival of a solid organ allograft is limited. Experimental research on naturally occurring CD4(+)CD25(high)FoxP3(+) Regulatory T cells (Tregs) has indicated their potential to establish stable long-term graft acceptance, with the promise of providing a more effective therapy for transplant recipients. Current approaches for clinical use are based on the infusion of freshly isolated or ex vivo polyclonally expanded Tregs into graft recipients with an aim to redress the in vivo balance of T effector cells to Tregs. However mounting evidence suggests that regulation of donor-specific immunity may be central to achieving immunological tolerance. Therefore, the next stages in optimizing translation of Tregs to organ transplantation will be through the refinement and development of donor alloantigen-specific Treg therapy. The altering kinetics and intensity of alloantigen presentation pathways and alloimmune priming following transplantation may indeed influence the specificity of the Treg required and the timing or frequency at which it needs to be administered. Here we review and discuss the relevance of antigen-specific regulation of alloreactivity by Tregs in experimental and clinical studies of tolerance and explore the concept of delivering an optimal Treg for the induction and maintenance phases of achieving transplantation tolerance.
Point pattern matching using convex hull edges (1985)

Algorithms for matching two sets of points in a plane are given. These algorithms search in the parameter space and find the transformation parameters that can match the most points in two sets. Since an exhaustive search for the best parameters is not affordable as the number of points in the sets become large, a subset selection method is given in order to reduce the search domain. Subsets are chosen as points on the boundary of the convex hulls of the sets. The algorithms are tested on generated and real data, and their performance is compared.
The Apolipoprotein E E4 Allele and Sex-Specific Risk of Alzheimer's Disease (1995)

<h3>To the Editor.</h3> —Recently, Dr Payami and colleagues proposed a difference in Alzheimer's disease (AD) risk between men and women who have one copy of the apolipoprotein E (apo E) E4 allele.<sup>1</sup>Specifically, they suggested that men with no or one copy of<i>E4</i>(the<i>E2/E2, E2/E3, E3/E3, E2/E4</i>, and<i>E3/E4</i>genotypes) have a decreased risk of AD compared with men with two copies (the<i>E4/E4</i>genotype), while women with no copy of<i>E4</i>have a decreased risk of AD compared with those with one or two copies. Our studies do not suggest this sex-specific pattern of risk.<sup>2,3</sup>We find instead that risk of AD, both familial and sporadic (ie, no family history), increases with the number of<i>E4</i>alleles in men and women. The<i>E4</i>gene dose effect was initially found in 234 members of 42 AD families older than 60 years and can be seen when
Fracture strength of polysilicon at stress concentrations (2003)

Mechanical design of MEMS requires the ability to predict the strength of load-carrying components with stress concentrations. The majority of these microdevices are made of brittle materials such as polysilicon, which exhibit higher fracture strengths when smaller volumes or areas are involved. A review of the literature shows that the fracture strength of polysilicon increases as tensile specimens get smaller. Very limited results show that fracture strengths at stress concentrations are larger. This paper examines the capability of Weibull statistics to predict such localized strengths and proposes a methodology for design. Fracture loads were measured for three shapes of polysilicon tensile specimens - with uniform cross-section, with a central hole, and with symmetric double notches. All specimens were 3.5 /spl mu/m thick with gross widths of either 20 or 50 /spl mu/m. A total of 226 measurements were made to generate statistically significant information. Local stresses were computed at the stress concentrations, and the fracture strengths there were approximately 90% larger than would be predicted if there were no size effect (2600 MPa versus 1400 MPa). Predictions based on mean values are inadequate, but Weibull statistics are quite successful. One can predict the fracture strength of the four shapes with stress concentrations to within /spl plusmn/10% from the fracture strengths of the smooth uniaxial specimens. The specimens and test methods are described and the Weibull approach is reviewed and summarized. The CARES/Life probabilistic reliability program developed by NASA and a finite element analysis of the stress concentrations are required for complete analysis. Incorporating all this into a design methodology shows that one can take "baseline" material properties from uniaxial tensile tests and predict the overall strength of complicated components. This is commensurate with traditional mechanical design, but with the addition of Weibull statistics.
FP tethering: a screening technique to rapidly identify compounds that disrupt protein–protein interactions (2014)

Fluorescence polarization (fp) tethering: a rapid and inexpensive strategy for fragment ligand discovery.
Recombinant Porcine Zona Pellucida Glycoproteins Expressed in Sf9 Cells Bind to Bovine Sperm but Not to Porcine Sperm (2005)

The zona pellucida, which surrounds the mammalian oocyte, consists of the ZPA, ZPB, and ZPC glycoproteins and plays roles in species-selective sperm-egg interactions via its carbohydrate moieties. In the pig, this activity is conferred by tri- and tetraantennary complex type chains; in cattle, it is conferred by a chain of 5 mannose residues. In this study, porcine zona glycoproteins were expressed as secreted forms, using the baculovirus-Sf9 insect cell system. The sperm binding activities of the recombinant proteins were examined in three different assays. The assays clearly demonstrated that recombinant ZPB bound bovine sperm weakly but did not bind porcine sperm; when recombinant ZPC was also present, bovine sperm binding activity was greatly increased, but porcine sperm still was not bound. The major sugar chains of ZPB were pauci and high mannose type chains that were similar in structure to the major neutral N-linked chain of the bovine zona. In fact, the nonreducing terminal alpha-mannose residues were necessary for the sperm binding activity. These results show that the carbohydrate moieties of zona glycoproteins, but not the polypeptide moieties, play an essential role in species-selective recognition of porcine and bovine sperm. Moreover, Asn to Asp mutations at either of two of the N-glycosylation sites of ZPB, residue 203 or 220, significantly reduced the sperm binding activity of the ZPB/ZPC mixture, whereas a similar mutation at the third N-glycosylation site, Asn-333, had no effect on binding. These results suggest that the N-glycans located in the N-terminal half of the ZP domain of porcine ZPB are involved in sperm-zona binding.
A Comparative Study of Styrene Polymerization in Deproteinized and Undeproteinized Natural Rubber Latex (2002)

Abstract An investigation on in situ polymerization of styrene in deproteinized natural rubber (DPNR) latex and high ammonia natural rubber (HANR) latex was carried out. The ratio of styrene to dry rubber was fixed at 25 : 75 by weight. It was observed that, at reaction temperature of 60 °C and reaction time of 10 hours, the styrene-DPNR system could attain a high conversion of 97% without adding surfactant. The degree of chemical bonding estimated for the resultant polystyrene-DPNR dried material was about 80%. The styrene-HANR system, however, required the addition of surfactant to complete the reaction time; but the conversion was found to be relatively low, in the region of 66%. This low conversion could be attributed to the role played by the protein/lipid layer, which is virtually absent in the styrene-DPNR system.
The blood‐brain barrier: Morphology, molecules, and neurothelin (1993)

The blood-brain barrier (BBB) is a complex structure formed by vascular endothelial cells, which serve to stabilize the homeostasic processes that are essential for neural functioning. The barrier relies on tight junctions between neighboring endothelial cells and a highly restricted passage of blood-borne components through the endothelial lining. Selective transport mechanisms guarantee the essential import and export of metabolites through the BBB into and out of the neural microenvironment. The dual functions of barrier and carrier depend on distinct proteins, some of which have been characterized in detail.
Novel data processing techniques for dispersive white light interferometer (2003)

White light interferometry has been used in the sensing area for many years. A novel data processing method for demodulating the information from the interference spectrum of a white light system is presented. Compared with traditional algorithms, both high-resolution and large dynamic range have been achieved with a relatively low-cost system. Details of this arithmetic are discussed. A compact white light interferometric system employing this algorithm has been developed, combined with fiber Fabry-Perot sensors. A60.5-nm stability over 48 hours with a dynamic range on the order of tens of microns has been achieved with this system. The temperature dependence of this system has been analyzed, and a self-compensating data processing approach is adopted. Experimental results demonstrated a 61.5-nm shift in the temperature range of 10 to 45°C. © 2003 Society of Photo-Optical Instrumentation Engineers. (DOI: 10.1117/1.1613958)
Conjunctivitis (1997)

In the first in a new series on common complaints presenting to the practice nurse, Wendy and Stephen Johnson look at the examination and treatment of conjunctivitis.
Comparison of Gen-Probe Transcription-Mediated Amplification, Abbott PCR, and Roche PCR Assays for Detection of Wild-Type and Mutant Plasmid Strains of <i>Chlamydia trachomatis</i> in Sweden (2008)

The clinical performance of two nucleic acid amplification assays targeting the cryptic plasmid and two assays targeting rRNA molecules in Chlamydia trachomatis was examined. First-catch urine samples from Malmoe, Sweden, were tested for C. trachomatis with the Abbott real-time PCR assay m2000 and an in-house PCR for the new variant strain of C. trachomatis with a deletion in the cryptic plasmid. Aliquots of the urine samples were sent to Aarhus, Denmark, and further examined with the Roche COBAS Amplicor CT (RCA) PCR, the Gen-Probe Aptima Combo 2 assay (AC2) targeting the C. trachomatis 23S rRNA, and the Aptima C. trachomatis assay (ACT) targeting the 16S rRNA molecule. A positive prevalence of 9% (163/1,808 urine samples examined) was detected according to the combined reference standard. The clinical sensitivity and specificity of the four assays were as follows: for ACT, 100% (163/163) and 99.9% (1,643/1,645), respectively; for AC2, 100% (163/163) and 99.6% (1,640/1,645); for m2000, 68.7% (112/163) and 99.9% (1,644/1,645); for RCA, 63.8% (104/163) and 99.9% (1,643/1,645). The two Gen-Probe assays detected all mutant strains characterized by the in-house PCR as having the deletion in the cryptic plasmid, whereas the Roche and the Abbott PCRs targeting the plasmid were both unable to detect the plasmid mutant. The difference in clinical sensitivity between the plasmid PCR assays m2000 and RCA, on the one hand, and the rRNA assays AC2 and ACT, on the other, could be attributed almost exclusively to the presence of the plasmid mutant in about one-quarter of the Chlamydia-positive samples examined.
Oracle Complexity Classes and Local Measurements on Physical Hamiltonians (2019)

The canonical hard problems for NP and its quantum analogue, Quantum Merlin-Arthur (QMA), are MAX-k-SAT and the k-local Hamiltonian problem (k-LH), the quantum generalization of MAX-k-SAT, respectively. In recent years, however, an arguably even more physically motivated problem than k-LH has been formalized - the problem of simulating local measurements on ground states of local Hamiltonians (APX-SIM). Perhaps surprisingly, [Ambainis, CCC 2014] showed that APX-SIM is likely harder than QMA. Indeed, [Ambainis, CCC 2014] showed that APX-SIM is P^{QMA[log]}-complete, for P^{QMA[log]} the class of languages decidable by a P machine making a logarithmic number of adaptive queries to a QMA oracle. In this work, we show that APX-SIM is P^{QMA[log]}-complete even when restricted to physically motivated Hamiltonians, obtaining as intermediate steps a variety of related complexity-theoretic results. Specifically, we first give a sequence of results which together yield P^{QMA[log]}-hardness for APX-SIM on well-motivated Hamiltonians such as the 2D Heisenberg model: - We show that for NP, StoqMA, and QMA oracles, a logarithmic number of adaptive queries is equivalent to polynomially many parallel queries. Formally, P^{NP[log]}=P^{||NP}, P^{StoqMA[log]}=P^{||StoqMA}, and P^{QMA[log]}=P^{||QMA}. (The result for NP was previously shown using a different proof technique.) These equalities simplify the proofs of our subsequent results. - Next, we show that the hardness of APX-SIM is preserved under Hamiltonian simulations (à la [Cubitt, Montanaro, Piddock, 2017]) by studying a seemingly weaker problem, ∀-APX-SIM. As a byproduct, we obtain a full complexity classification of APX-SIM, showing it is complete for P, P^{||NP},P^{||StoqMA}, or P^{||QMA} depending on the Hamiltonians employed. - Leveraging the above, we show that APX-SIM is P^{QMA[log]}-complete for any family of Hamiltonians which can efficiently simulate spatially sparse Hamiltonians. This implies APX-SIM is P^{QMA[log]}-complete even on physically motivated models such as the 2D Heisenberg model. Our second focus considers 1D systems: We show that APX-SIM remains P^{QMA[log]}-complete even for local Hamiltonians on a 1D line of 8-dimensional qudits. This uses a number of ideas from above, along with replacing the "query Hamiltonian" of [Ambainis, CCC 2014] with a new "sifter" construction.
An Adaptive-Rate Error Correction Scheme for NAND Flash Memory (2009)

ECC has been widely used to enhance flash memory endurance and reliability. In this work, we propose an adaptive-rate ECC scheme with BCH codes that is implemented on the flash memory controller. With this scheme, flash memory can trade storage space for higher error correction capability to keep it usable even when there is a high noise level.
ATF3 Protects against Renal Ischemia-Reperfusion Injury (2008)

Oxidative stress-induced cell death plays a major role in the progression of ischemic acute renal failure. Using microarrays, we sought to identify a stress-induced gene that may be a therapeutic candidate. Human proximal tubule (HK2) cells were treated with hydrogen peroxide (H2O2) and RNA was applied to an Affymetrix gene chip. Five genes were markedly induced in a parallel time-dependent manner by cluster analysis, including activating transcription factor 3 (ATF3), p21(WAF1/CiP1) (p21), CHOP/GADD153, dual-specificity protein phosphatase, and heme oxygenase-1. H2O2 rapidly induced ATF3 approximately 12-fold in HK2 cells and approximately 6.5-fold in a mouse model of renal ischemia-reperfusion injury. Adenovirus-mediated expression of ATF3 protected HK2 cells against H2O2-induced cell death, and this was associated with a decrease of p53 mRNA and an increase of p21 mRNA. Moreover, when ATF3 was overexpressed in mice via adenovirus-mediated gene transfer, ischemia-reperfusion injury was reduced. In conclusion, ATF3 plays a protective role in renal ischemia-reperfusion injury and the mechanism of the protection may involve suppression of p53 and induction of p21.