gebhart/patrepeval huggingface dataset:
Purification and characterization of M-177, a 177 kDa allergen, from the house dust mite Dermatophagoides farinae (1999)

A high molecular weight allergen, M-177, was recently discovered in the house dust mite, Dermatophagoides farinae (D. farinae). The aims of this study were to develop a conventional purification procedure for M-177 and then to analyze some of the immunochemical properties of M-177. Mite extracts obtained from purified mite bodies were a suitable material for preparing M-177, because the purified mite extract contained large amounts of M-177. The purification of this allergen from the extract was achieved by ethanol fractionation, anion exchange chromatography, and gel filtration chromatography. The purified antigen was immunochemically equivalent to that of a preparation obtained by a previous affinity method using an anti-Mag 3-immobilized column. The yield of this purification procedure was 36.8% of the initial amount of M-177 in the extract, 40-fold greater than that of the previous immunoaffinity method. Our purification method was useful for preparing this allergen. The purified M-177 reacted in skin tests in 11 of 16 miteallergic patients, compared to 10 of 16 with Der f 2. The amount of M-177 in the purified mite extract determined by enzyme-linked immunosorbent assay inhibition was as much as 0.95% of the total protein, which was higher than the amounts of Der f 1 (0.52%) and Der f 2 (0.32%). The potent allergenic activity and large amount of M-177 in the mites indicate that it is an important mite allergen.
Enhanced Aspect Ratio of Focused Ion Beam Nanopatterning Technique in Semiconductors (2007)

We demonstrate a more than 10 aspect-ratio FIB semiconductor nanopatterning technique. The undesired semiconductor material decomposition by the beam-tail ions is prevented by a protective Ti layer acting as a mask for the semiconductor.
One‐Component Catalysts for Thermal and Photoinduced Ring Opening Metathesis Polymerization (1997)

[Ru(p-cymene)Cl2(PCy3)] was identified as a latent, very tolerant, one-component catalyst for the bulk polymerization of dicyclopentadiene (DCPD). The robustness of this catalyst in combination with the interesting properties of filled or unfilled poly(DCPD) allows the preparation of novel thermosets (structure shown on the right).
Characterization of the cDNA of a broadly reactive neutralizing human anti-gp120 monoclonal antibody. (1992)

The F105 mAb, identified in an HIV-1-infected individual, binds to a discontinuous epitope on the HIV-1 gp120 envelope glycoprotein, blocks the binding of gp120 to the CD4 viral receptor, and neutralizes a broad range of HIV-1 isolates. This study reports the primary nucleotide and deduced amino acid sequences of the rearranged heavy and light chains of the mAb F105. This IgG1k mAb uses a VH gene member of the VH4 gene family (V71-4) and is productively rearranged with a D-D fusion product of the dlr4 and da4 germline DH genes and the JH5 gene. This rearranged heavy chain gene expresses the VH4-HV2a idiotope, which is seen in human monoclonal IgM cold agglutinins. The F105 Vk appears to be derived from the Humvk325 germline gene and is rearranged with a Jk2 gene. For both chains, the mutational pattern in the rearranged VH and VL genes is indicative of an antigen-driven process. These studies show that production of a broadly neutralizing anti-HIV-1 antibody that recognizes determinants within the CD4 recognition site of the envelope glycoprotein is achieved by rearrangement of the V71-4 and Humvk325 germline variable region genes along with selected individual point mutations in the rearranged genes.
NF-κB, p38 MAPK, ERK1/2, mTOR, STAT3 and increased glycolysis regulate stability of paricalcitol/dexamethasone-generated tolerogenic dendritic cells in the inflammatory environment (2015)

Tolerogenic dendritic cells (tDCs) may offer an intervention therapy in autoimmune diseases or transplantation. Stable immaturity and tolerogenic function of tDCs after encountering inflammatory environment are prerequisite for positive outcome of immunotherapy. However, the signaling pathways regulating their stable tolerogenic properties are largely unknown. In this study, we demonstrated that human monocyte-derived tDCs established by using paricalcitol (analogue of vitamin D2), dexamethasone and monophosphoryl lipid A exposed for 24h to LPS, cytokine cocktail, polyI:C or CD40L preserved reduced expression of co-stimulatory molecules, increased levels of inhibitory molecules ILT-3, PDL-1 and TIM-3, increased TLR-2, increased secretion of IL-10 and TGF-β, reduced IL-12 and TNF-α secretion and reduced T cell stimulatory capacity. tDCs further induced IL-10-producing T regulatory cells that suppressed the proliferation of responder T cells. In the inflammatory environment, tDCs maintained up-regulated indoleamine 2, 3 dioxygenase but abrogated IκB-α phosphorylation and reduced transcriptional activity of p65/RelA, RelB and c-Rel NF-κB subunits except p50. Mechanistically, p38 MAPK, ERK1/2, mTOR, STAT3 and mTOR-dependent glycolysis regulated expression of ILT-3, PDL-1 and CD86, secretion of IL-10 and T cell stimulatory capacity of tDCs in the inflammatory environment. Stability of tDCs in the inflammatory environment is thus regulated by multiple signaling pathways.
New Strategies for Activation of Phosphonates/Phosphates to Forge Functional Phosphorus Compounds (2019)

Organophosphonate analogues are important structural motifs that are present in bioactive natural products, pharmaceuticals, and agrochemicals. Because they are useful in a broad range of applications, much effort has focused on developing efficient synthetic methods that enable access to organophosphonates and their derivatives. However, currently available synthetic procedures rely on harsh reaction conditions and are limited to a narrow substrate scope. Our lab has recently made important advances in leveraging inert phosphonates/phosphates into functional phosphorus compounds such as mixed phosphonates, phosphates, and mixed aryl phosphate derivatives. Presented herein is an overview of recent achievements in the synthesis of phosphonate/phosphate derivatives and a summary of our recent accomplishments in Tf2O-promoted activating strategy of phosphonate analogues. 1 Introduction 2 Direct Activating Strategy of Phosphonate Analogues 3 Late-stage Phosphonylation of Natural Compounds 4 Potential Applications 5 Summary
Acoustic emission transducer and mounting adapted for monitoring metalcutting tools (1991)

First Page
Ground vehicle doppler geolocation (2014)

This paper reports on the performance of a recently developed particle filter technique to locate a stationary push-to-talk (PTT) radio using the Doppler-shifted signal as observed by a single moving ground vehicle. The primary difficulty for single-sensor Doppler-based localization is that the solution is strongly dependent on the target's unknown original carrier frequency. This uncertainty allows many possible location estimates to correspond with the observations. Furthermore, the PTT carrier is observed to drift significantly over short time periods. This creates a dynamic, multimodal objective function and motivates the application of the particle filter solution. The technique's performance is demonstrated on real data collected by a slow-moving (10-40 mph) ground vehicle over a relatively short 0.5 km range. The location accuracies achieved are compared to the theoretical frequency of arrival (FOA) Cramer-Rao Lower Bound (CRLB) and with the more common two sensor frequency difference of arrival (FDOA) solution.
When cloud virtual machine images need to be updated (2013)

Updates to cloud images typically come in the form of patches and either correct bugs and security vulnerabilities or introduce new functionality. The complexity and effort required to patch an image is much higher than what is required to patch an instance. This is due to the risk of incorrectly modifying configurations, breaking the cloud provisioning for the image or preventing the correct operation of the management stack. In a managed cloud, if a patch is not applied to an image it must be applied to each instance of the image. This process results in wastage of compute resources and causes the customer to receive an initial instance that has not been tested by the cloud provider. This paper proposes an algorithm to identify when an image should be updated based on the frequency of instantiation requests and the outstanding patches as actually experienced in a production data centre.
In Vitro Development of Ovarian Follicles (2011)

Tissue banking of ovarian material is being increasingly offered to a variety of patients as a means of fertility preservation. This tissue comprises thin cortical surface biopsies that contain predominantly primordial follicles, and currently the only option to restore fertility is by transplantation. However, this is not a viable option for all patients. The potential of this tissue could be realized by the development of in vitro systems to support complete growth from the early primordial stages through to maturity. This technology would have many therapeutic applications including the production of competent oocytes for assisted reproduction technologies, determination of toxicological effects on germ cell development, assessment of cryopreserved ovarian tissue before transplantation for fertility preservation as well as providing an experimental model to address basic scientific questions concerning human oocyte development. Complete oocyte development in vitro from the primordial stage has been achieved in mice, but the larger size and longer growth period of human follicles has made the interspecies translation of these techniques difficult. Recently progress has been made in defining conditions that support different stages of follicle development in vitro that make a complete in vitro system from primordial to maturation a possible reality. This article deals with our current understanding of in vitro development.
Implication des composés de surface dans la modulation de l’inflammation intestinale par [i]Propionibacterium freudenreichii.[/i] (2013)

Propionibacterium freudenreichii est une bacterie de l’ordre des Actinomycetales qui beneficie des statuts GRAS et QPS. Consommee en grande quantite (1010 CFU/jour/personne en France) dans les fromages de type Emmental, elle est egalement commercialisee en tant que probiotique. Cette etude vise a mettre en evidence les proprietes immunomodulatrices d’une telle consommation, dans un contexte ou les maladies intestinales inflammatoires constituent un probleme de sante publique croissant. Sujets / materiels et methodes : Les proprietes immunomodulatrices de 20 souches de P. freudenreichii ont ete comparees in vitro dans un modele de PBMC humains, puis in vivo dans deux modeles de colite experimentale chez la souris. Un lait fermente exclusivement par P. freudenreichii a ete mis au point et teste dans un modele de porcelet sain. Une approche multidisciplinaire (purification, mutation, expression heterologue, evaluation in vitro et in vivo) precise les mecanismes mis en jeux. Resultats principaux : Nous avons mis en evidence des proprietes immunomodulatrices souche-dependantes pour cette bacterie, en terme de stimulation de cytokines et de protection vis-a-vis de colite experimentale chez la souris. Le lait fermente mis au point a eu un effet promoteur de sante chez le porcelet et delivre P. freudenreichii vivant et metaboliquement active dans le colon. Sa consommation reprime la production de la cytokine pro-inflammatoire IL-8 au niveau de la muqueuse intestinale porcine. Une approche mecanistique a revele les roles contrastes de differents composes de surface tels que les exopolysaccharides et les proteines de surface, dans l’immunomodulation. Certaines proteines sont impliquees dans la modulation du systeme immunitaire, alors que la capsule exopolysaccharidique annule cette modulation. Conclusions : Le role de chaque molecule est actuellement aborde dans un projet ANR (Sarter SURFace against INflammation of the Gut) par une approche multidisciplinaire mobilisant 7 partenaires. L’elucidation des mecanismes cellulaires et moleculaires induits, ainsi que les effets observes in vivo, ouvriront des perspectives pour l’utilisation de produits laitiers fermentes par P. freudenreichii repondant aux besoins specifiques de populations ciblees.
Memristor crossbar based multicore neuromorphic processors (2014)

This paper describes memristor-based neuromorphic circuits for non-linear separable pattern recognition. We initially describe a memristor based neuron circuit and then show how multilayer neural networks can be constructed based on this neuron circuit. By applying neural network learning algorithms to these circuits, we demonstrate the learning of both linearly and non-linearly separable logic functions. The simulations are carried out in SPICE using a detailed memristor model so that the crossbar is simulated as accurately as possible. We also examine the system level performance of multicore memristor crossbar based neuromorphic processors. We consider the impact on on-chip routing, calculate the chip areas, and evaluate the timing of the systems in the study. The results indicate that such architectures can enable over 300,000 times energy efficiencies over traditional high performance computing architectures when processing large neural networks.
A technique for identifying microseismic multiplets and application to the Valhall field, North Sea (2006)

Abstract A fast, fully automatic technique to identify microseismic multiplets in borehole seismic data is developed. The technique may be applied in real time to either continuous data or detected-event data for a number of three-component receivers and does not require prior information such as P- or S-wave time picks. Peak crosscorrelation coefficients, evaluated in the frequency domain, are used as the basis for identifying microseismic doublets. The peak crosscorrelation coefficient at each receiver is evaluated with a weighted arithmetic average of the normalized correlation coefficients of each component. Each component is weighted by the maximum amplitude of the signal for that component to reduce the effect of noise on the calculations. The weighted average correlations are averaged over all receivers in a time window centered on a fixed lag time. The size of the time window is determined from the dominant period in the signal, and the lag time is the time that maximizes the average correlation coefficient. The technique is applied to a three-component passive seismic data set recorded at the Valhall field, North Sea. A large number of microseismic doublets are identified that can be grouped into multiplets, reducing the total number of absolute event locations by a factor of two. Seven large multiplets reflect the repeated multiple rerupturing (up to 30 times on a single fault) and significant stress release. Two major faults dominate the seismic activity, causing at least one-fourth of the observed events.
The Role of Pressure in the Study of Organic Superconductors (1990)

The impact of high pressure in the study of organic superconductivity is reviewed. Studies of the pressure dependence of the superconducting transition temperature of the (ET){sub n}X{sub m} family using solid He pressure techniques and high quality single crystal samples are discussed in detail. 28 refs., 2 figs.
Will your facebook post be engaging? (2013)

Social media has become the ideal platform for promotional activities of organizations. However, due to the volatility of social media, the wrong message posted at the wrong time can result in significant damage to hard-built brand image. This calls for a mechanism to gauge the reactions a post will evoke from a given social community. The community can vary from customers of a particular brand to brand loyalists interacting through its social pages (for example, on Facebook). In this paper, we focus on learning the community's reaction from past posts and providing a predictive model for gauging the reaction of the community before the post is published. This helps the marketer take better-informed decisions. Short-text posts in social media leads to a sparse feature space, we propose additional meta-features that improve reaction modeling. Given the feature representation, we discuss the possibility of casting the underlying problem under different paradigms - classification, regression and learning to rank. We study the performances of each of these paradigms on real data from Facebook. We will discuss the challenges involved, and ways to mitigate them, in addition to our observations, results and insights.
OSPF-TE Extensions for General Network Element Constraints (2015)

Generalized Multiprotocol Label Switching (GMPLS) can be used to control a wide variety of technologies including packet switching (e.g., MPLS), time division (e.g., Synchronous Optical Network / Synchronous Digital Hierarchy (SONET/SDH) and Optical Transport Network (OTN)), wavelength (lambdas), and spatial switching (e.g., incoming port or fiber to outgoing port or fiber). In some of these technologies, network elements and links may impose additional routing constraints such as asymmetric switch connectivity, nonlocal label assignment, and label range limitations on links. This document describes Open Shortest Path First (OSPF) routing protocol extensions to support these kinds of constraints under the control of GMPLS.
Low-delay code-excited linear-predictive coding of wideband speech at 32 kbps (1991)

The authors report on the use of the codebook-excited linear-predictive (CELP) algorithm for 32 kb/s low-delay (LD-CELP) coding of wideband speech. The main problem associated with wideband coding, namely, spectral noise weighting, is discussed. The authors propose an enhanced noise weighting technique and demonstrate its efficiency via subjective listening tests. In these tests, involving 20 listeners and 8 test sentences, the average rating for the proposed 32 kb/s LD-CELP was essentially equal to that of the 65 kb/s standard (G.722) CCITT wideband coder.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Dispersed Mobile-Phase Countercurrent Chromatography (2016)

Countercurrent distribution based on liquid–liquid partition is a powerful separation method with minimal incurrence of loss of solutes, but its industrial application has been limited by cumbersome shifting of immiscible solvents. Although centrifugation has been employed to facilitate equilibration between phases, process scaling-up remains difficult. In this study, a dispersed mobile-phase countercurrent chromatography (DMCC) method has been developed to adapt the countercurrent distribution principle to a continuous column chromatography format. Continuous solute exchange between two immiscible phases within a series of separation columns is achieved by mechanical dispersion of an influx of mobile phase into an upward stream of small droplets travelling through the columns filled with stationary phase. The diameter, length, and number of columns, and the number of stationary phases employed in the different columns can be varied to match the requisite scale and resolution of operation. Illustrations of DMCC were provided by examples of solute separations where the fractionated solutes could be collected either from the eluate of the series of columns, or from drainage of the stationary phases in the individual columns at the end of a chromatographic run.
Biological substrate modification suppresses ventricular arrhythmias in a porcine model of chronic ischaemic cardiomyopathy (2022)

Biological substrate modification by exosome injection may be worth developing as a non-destructive alternative to conventional ablation for the prevention of recurrent ventricular tachyarrhythmias.
Genomic organization of the human CYP3A locus: identification of a new, inducible CYP3A gene (2001)

Proteins encoded by the human CYP3A genes metabolize every second drug currently in use. The activity of CYP3A gene products in the general population is highly variable and may affect the efficacy and safety of drugs metabolized by these enzymes. The mechanisms underlying this variability are poorly understood, but they include gene induction, protein inhibition and unknown genetic polymorphisms. To better understand the regulation of CYP3A expression and to provide a basis for a screen of genetic polymorphisms, we determined and analysed the sequence of the human CYP3A locus. The 231 kb locus sequence contains the three CYP3A genes described previously (CYP3A4, CYP3A5 and CYP3A7), three pseudogenes as well as a novel CYP3A gene termed CYP3A43. The gene encodes a putative protein with between 71.5% and 75.8% identity to the other CYP3A proteins. The highest expression level of CYP3A43 mRNA is observed in the prostate, an organ with extensive steroid metabolism. CYP3A43 is also expressed in several other tissues including liver, where it can be induced by rifampicin. CYP3A43 transcripts undergo extensive splicing. The identification of a new member of the CYP3A family and the characterization of the full CYP3A locus will aid efforts to identify the genetic variants underlying its variable expression. This, in turn, will lead to a better optimization of therapies involving the numerous substrates of CYP3A proteins.
Quantum Cascade Whispering Gallery Lasers (1997)

Low threshold, single-mode quantum cascade whispering gallery lasers with emission wavelengths from 5.0 to 11.5 micrometer are reported. Their potential for true microcavities is discussed.
Photoluminescence: a surprisingly sensitive lifetime technique (2005)

Photoluminescence (PL) measurements in transient, quasi steady state and in an intermediate mode are used to determine the injection level dependent effective excess carrier lifetime /spl tau//sub eff/ in silicon wafers. The effective lifetime is measured over a wide dynamic range and down to very small injection levels /spl Delta/n < 10/sup 9/ cm/sup -3/, demonstrating that PL is a convenient and also a surprisingly sensitive lifetime technique. We show experimentally that PL measurements are not significantly affected by excess carriers accumulated in space charge regions in contrast to other lifetime techniques such as photoconductance (PC). With PL measurements on bifacial silicon solar cells it is also demonstrated experimentally that simultaneous measurements of PL and of the incident light intensity yield the equivalent of Suns-V/sub oc/ measurements, but in contact less mode and without the need for a solar cell structure.
Comparative EPR and fluorescence conformational studies of fully active spin‐labeled melanotropic peptides (2001)

Similar to melanocyte stimulating hormone (alpha-MSH), its potent and long-acting analogue, [Nle(4), D-Phe(7)]alpha-MSH, when labeled with the paramagnetic amino acid probe 2,2,6,6-tetramethylpiperidine-N-oxyl-4-amino-4-carboxylic acid (Toac), maintains its full biological potency, thus validating any comparative structural investigations between the two labeled peptides. Correlation times, calculated from the electron paramagnetic resonance signal of Toac bound to the peptides, and Toac-Trp distances, estimated from the Toac fluorescence quenching of the Trp residue present in the peptides, indicate a more rigid and folded structure for the potent analogue as compared to the hormone, in aqueous medium.