gebhart/patrepeval huggingface dataset:
Expression and purification of active form of HIV-1 protease from E.coli. (1995)

We have subcloned an N-terminal extended protease gene of human immunodeficiency virus (HIV) type 1 that is encoded in the protease domain of the pol open reading frame into expression vector pGEX-KG. A relatively high level of expression of recombinant HIV-1 protease (PR) was achieved with isopropyl beta-D-thiogalactoside (IPTG) induction and glucose supplement. An isolation method consisting of denaturation of protein and followed by refolding was developed for releasing this recombinant HIV-1 PR into the soluble phase since most of the expressed protease was initially present in insoluble inclusion bodies. High purity of this recombinant HIV-1 PR was obtained by sequential purification using Sephadex G-50 gel filtration and CM-23 cellulose cation exchange chromatography, yielding the protease more than 1 mg per liter culture. N-terminal amino acid sequence analysis showed that the recombinant HIV-1 PR underwent autocleavage from the fusion protein during expression. SDS-PAGE indicated that the molecular weight of this recombinant HIV-1 PR is 11 kDa. This recombinant HIV-1 PR showed proteolytic activity for the synthetic peptide substrates corresponding to the sequence at the Gag MA/CA and Pol p6*/PR junctions. The purified enzyme whose specific activity for the heptapeptide SQNYPIV was 848.7 nmol*min-1*mg protease-1 also processed recombinant polyprotein Gag41 as its substrate.
Photoeffect in porous silicon (1997)

A new contactless technique makes possible the measurement of spectral dependences of the photoeffect and the photoeffect time relaxations in porous silicon. From these photoeffect measurements the following results have been obtained. The onset of excess carrier generation at about 1.35 eV was found. The photoeffect time relaxation constant ranges from 0.15 to 10 ms. The dominant components of the measured photoeffect were produced in the depletion space charge regions near both surfaces. Strong trapping of excess carriers near the sample surfaces was observed.
Biochemically controlled thermal phase transition of gels (1991)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTBiochemically controlled thermal phase transition of gelsEtsuo Kokufuta and Toyoichi TanakaCite this: Macromolecules 1991, 24, 7, 1605–1607Publication Date (Print):April 1, 1991Publication History Published online1 May 2002Published inissue 1 April 1991https://pubs.acs.org/doi/10.1021/ma00007a024https://doi.org/10.1021/ma00007a024research-articleACS PublicationsRequest reuse permissionsArticle Views182Altmetric-Citations60LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Identification of NKp80, a novel triggering molecule expressed by human NK cells (2001)

The ability of NK cells to kill a wide range of tumor or virally infected target cells as well as normal allogeneic T cell blasts appears to depend upon the concerted action of multiple triggering NK receptors. In this study, using two specific monoclonal antibodies [(mAb) MA152 and LAP171], we identified a triggering NK receptor expressed at the cell surface as a dimer of approximately 80 kDa (NKp80). NKp80 is expressed by virtually all fresh or activated NK cells and by a minor subset of T cells characterized by the CD56 surface antigen. NKp80 surface expression was also detected in all CD3- and in 6 / 10 CD3+ large granular lymphocyte expansions derived from patients with lymphoproliferative disease of granular lymphocytes. In polyclonal NK cells, mAb-mediated cross-linking of NKp80 resulted in induction of cytolytic activity and Ca2+ mobilization. A marked heterogeneity existed in the magnitude of the cytolytic responses of different NK cell clones to anti-NKp80 mAb. This heterogeneity correlated with the surface density of NKp46 molecules expressed by different NK clones. The mAb-mediated masking of NKp80 led to a partial inhibition of the NK-mediated lysis of appropriate allogeneic phytohemagglutinin-induced T cell blasts, while it had no effect on the lysis of different tumor target cells, including T cell leukemia cells. These data suggest that NKp80 recognizes a ligand on normal T cells that may be down-regulated during tumor transformation. Molecular cloning of the cDNA coding for NKp80 revealed a type II transmembrane molecule of 231 amino acids identical to the putative protein encoded by a recently identified cDNA termed KLRF1.
Handbook of Chemical and Biological Warfare Agents (1999)

Extensively revised and updated, this second edition of the bestselling Handbook of Chemical and Biological Warfare Agents goes well beyond thedirty thirty commonly discussed agents and provides rapid access to a wide range of agents that can be used as weapons. This edition incorporates additional classes of agents, expands existing clas
Current status of intravenous tissue plasminogen activator dosage for acute ischaemic stroke: an updated systematic review (2018)

The optimal dose of recombinant tissue plasminogen activator (rtPA) for acute ischaemic stroke (AIS) remains controversial, especially in Asian countries. We aimed to update the evidence regarding the use of low-dose versus standard-dose rtPA. We performed a systematic literature search across MEDLINE, Embase, Cochrane Central Register of Controlled Trials (CENTRAL), PsycINFO and Cumulative Index to Nursing and Allied Health Literature (CINAHL) from inception to 22 August 2016 to identify all related studies. The outcomes were death or disability (defined by modified Rankin Scale 2-6), death, and symptomatic intracerebral haemorrhage (sICH). Where possible, data were pooled for meta-analysis with ORs and corresponding 95% CIs by means of random-effects or fixed-effects meta-analysis. We included 26 observational studies and 1 randomised controlled trial with a total of 23 210 patients. Variable doses of rtPA were used for thrombolysis of AIS in Asia. Meta-analysis shows that low-dose rtPA was not associated with increased risk of death or disability (OR 1.13, 95% CI 0.95 to 1.33), or death (OR 0.86, 95% CI 0.74 to 1.01), or decreased risk of sICH (OR 1.06, 95% CI 0.65 to 1.72). The results remained consistent when sensitivity analyses were performed including only low-dose and standard-dose rtPA or only Asian studies. Our review shows small difference between the outcomes or the risk profile in the studies using low-dose and/or standard-dose rtPA for AIS. Low-dose rtPA was not associated with lower risk of death or disability, death alone, or sICH.
Quality assessment tools add value. (1996)

The rapid evolution of the health care marketplace can be expected to continue as we move closer to the 21st Century. Externally-imposed pressures for cost reduction will increasingly be accompanied by pressure within health care organizations as risk-sharing reimbursement arrangements become more commonplace. Competitive advantage will be available to those organizations that can demonstrate objective value as defined by the cost-quality equation. The tools an organization chooses to perform quality assessment will be an important factor in its ability to demonstrate such value. Traditional quality assurance will in all likelihood continue, but the extent to which quality improvement activities are adopted by the culture of an organization may determine its ability to provide objective evidence of better health status outcomes.
The clinical and immunological characteristics related to salivary gland enlargement in primary Sjögren’s syndrome: a retrospective cross-sectional study (2023)

SGE is highly consistent with increased immunological indicators, reflecting disease activity. pSS patients with SGE were younger than those without. Special attention should be paid to the changes in the anti-Ro52 level since it is an independent factor related to SGE in pSS.
Antibody Catalysis of Multistep Reactions: An Aldol Addition Followed by a Disfavored Elimination (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAntibody Catalysis of Multistep Reactions: An Aldol Addition Followed by a Disfavored EliminationThomas Koch, Jean-Louis Reymond, and Richard A. LernerCite this: J. Am. Chem. Soc. 1995, 117, 37, 9383–9387Publication Date (Print):September 1, 1995Publication History Published online1 May 2002Published inissue 1 September 1995https://pubs.acs.org/doi/10.1021/ja00142a001https://doi.org/10.1021/ja00142a001research-articleACS PublicationsRequest reuse permissionsArticle Views186Altmetric-Citations28LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (2)»Supporting Information Supporting Information Get e-Alerts
Weight gain in response to high-fat feeding in CD-1 male mice (2010)

The purpose of this study was to compare weight gain and food intake during high-fat feeding in outbred CD-1 male mice while considering several different experimental designs. This study was completed using data from three separate experiments and was designed to address different experimental design issues. Experiment 1 compared mice housed in groups or singly. Experiment 2 compared adolescent and young adult mice. Experiment 3 examined mice that had been previously exercise-trained prior to diet-induced weight gain. Data from each experiment were analysed using repeated measures analysis of variance and linear regression. While housing and age did not significantly affect weight gain, mice that were previously exercise-trained consumed significantly more kilocalories than sedentary mice while maintaining comparable body weights. We generated a linear prediction model using data from Experiments 1 and 2 that will allow investigators to calculate the weeks of high-fat feeding needed to reach a target body weight. Our key findings characterize the issues related to and affecting experimental design when utilizing an outbred mouse diet-induced weight gain model and will serve as a guide for future researchers.
Rapid, metal‐free room temperature vulcanization produces silicone elastomers (2012)

Abstract Silicone room temperature vulcanization elastomers are usually formed through either a platinum‐catalyzed hydrosilylation or tin‐catalyzed moisture cure. In this article, we show that it is possible to create robust, transparent silicone elastomers without the need for metal catalysts. Hydrogen‐terminated silicone polymers are crosslinked by tri‐ or tetraalkoxysilane crosslinkers in a condensation process catalyzed by the presence of trispentafluorophenylborane catalyst to give elastomers and alkane by‐products. This procedure allows for very fast cure times (< 30 s to a tack free state): the process is more conveniently controlled with the addition of a small amount of solvent. Physical and mechanical properties are readily modified by control of the chain length of the starting polymer, the functionality and nature of the alkoxy group on the crosslinker. Organofunctional groups, useful for further polymer modification, can optionally be incorporated by judicious choice of readily available starting materials. © 2012 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2013
LETTERS TO THE EDITOR (1999)

Suzuki, Kensuke MD; Takano, Shingo MD; Nose, Tadao MD; Doi, Mikio MD; Ohashi, Noriyoshi MD Author Information
Understanding IC lithography (1992)

Factors such as minimum resolution overlay tolerances and maximum topography are key elements of IC fabrication. Circuit designers must understand the techniques lithographers use in manufacturing ICs. An overview of the resist work performed at SEMATECH, including resist characterization, process control, and modeling, and a look at future lithography trends are presented. Swing curve, exposure/focus latitude, contrast, reflective notching, development rate, and plasma and thermal resistance measurement procedures for performing resist evaluations are discussed.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Cytokine and sex hormone effects on zidovudine- and lamivudine-triphosphate concentrations in vitro (2008)

We found evidence that sex hormones and cytokines influence zidovudine-triphosphate and lamivudine-triphosphate slightly in PBMCs and adipocytes in vitro. These findings provide insight and scientific direction to address inflammation-dependent and sex-dependent phosphorylation and responses in patients.
Immune evasion mediated by tumor-derived lactate dehydrogenase induction of NKG2D ligands on myeloid cells in glioblastoma patients (2014)

Myeloid cells are key regulators of the tumor microenvironment, governing local immune responses. Here we report that tumor-infiltrating myeloid cells and circulating monocytes in patients with glioblastoma multiforme (GBM) express ligands for activating the Natural killer group 2, member D (NKG2D) receptor, which cause down-regulation of NKG2D on natural killer (NK) cells. Tumor-infiltrating NK cells isolated from GBM patients fail to lyse NKG2D ligand-expressing tumor cells. We demonstrate that lactate dehydrogenase (LDH) isoform 5 secreted by glioblastoma cells induces NKG2D ligands on monocytes isolated from healthy individuals. Furthermore, sera from GBM patients contain elevated amounts of LDH, which correlate with expression of NKG2D ligands on their autologous circulating monocytes. NKG2D ligands also are present on circulating monocytes isolated from patients with breast, prostate, and hepatitis C virus-induced hepatocellular carcinomas. Together, these findings reveal a previously unidentified immune evasion strategy whereby tumors produce soluble factors that induce NKG2D ligands on myeloid cells, subverting antitumor immune responses.
Progress Toward the Structure and Therapeutic Use of the Hairpin Ribozyme (1997)

The hairpin ribozyme is one of a number of small catalytic RNAs that are excellent paradigms for RNA structure-function analysis and have potential also as therapeutic agents. This review outlines current understanding of the structure of the hairpin ribozyme and its basis for catalytic activity. Included also is a discussion of the functional group requirements for cleavage and the first steps being taken to understanding its folding. Finally, recent developments are highlighted in engineering the hairpin ribozyme for intracellular use as a potential gene therapy agent.
Cell stretching devices as research tools: engineering and biological considerations (2016)

Cells within the human body are subjected to continuous, cyclic mechanical strain caused by various organ functions, movement, and growth. Cells are well known to have the ability to sense and respond to mechanical stimuli. This process is referred to as mechanotransduction. A better understanding of mechanotransduction is of great interest to clinicians and scientists alike to improve clinical diagnosis and understanding of medical pathology. However, the complexity involved in in vivo biological systems creates a need for better in vitro technologies, which can closely mimic the cells' microenvironment using induced mechanical strain. This technology gap motivates the development of cell stretching devices for better understanding of the cell response to mechanical stimuli. This review focuses on the engineering and biological considerations for the development of such cell stretching devices. The paper discusses different types of stretching concepts, major design consideration and biological aspects of cell stretching and provides a perspective for future development in this research area.
효과적인 성인지도를 위한 실천적 전략―대학부설 평생교육원 성인학습자의 관점에서― (2004)

이 논문에서 필자는 효과적인 성인지도에 필요한 실천적 전략을 구안할 목적으로 수행된 탐색적 연구를 소개한다. 성인지도의 효과성에 대한 학습자의 인지 정도를 드러내기 위하여 교수 활동 시 성인 교수자가 실천해야 할 사항 (67문항)과 삼가야 할 사항 (47문항)에 관한 문항이 개발되었고, 각각의 문항은 성인학습자에 의해 그 중요도가 5점 척도로 평가되었다....
Quantitative Detection of Protein Arrays (2003)

We introduce a quantitative method that utilizes scanning electron microscopy for the analysis of protein chips (SEMPC). SEMPC is based upon counting target-coated gold particles interacting specifically with ligands or proteins arrayed on a derivative microscope glass slide by utilizing backscattering electron detection. As model systems, we quantified the interactions of biotin and streptavidin and of an antibody with its cognate hapten. Our method gives quantitative molecule-counting capabilities with an excellent signal-to-noise ratio and demonstrates a broad dynamic range while retaining easy sample preparation and realistic automation capability. Increased sensitivity and dynamic range are achieved in comparison to currently used array detection methods such as fluorescence, with no signal bleaching, affording high reproducibility and compatibility with miniaturization. Thus, our approach facilitates the determination of the absolute number of molecules bound to the chip rather than their relative amounts, as well as the use of smaller samples.
RNA Therapeutics Directed to the Non Coding Regions of APP mRNA, In Vivo Anti-Amyloid Efficacy of Paroxetine, Erythromycin, and N-acetyl cysteine (2006)

Lead compounds directed to the 5' leader of the Amyloid Precursor Protein transcript (i.e., paroxetine (SSRI), N-acetyl cysteine (antioxidant), and erythromycin (macrolide antibiotic)) were employed in a pilot study to evaluate their anti-amyloid efficacy in the TgCRND8 transgenic mouse model for Alzheimer's Disease (AD). The relative levels of Abeta peptide were reduced after exposure of mice to paroxetine (N=5), NAC (N=7), and erythromycin (N=7) relative to matched placebo counterparts. Paroxetine limited the levels of APP holoprotein and total Abeta peptide levels (measurements of Abeta were performed at two separate sites by quantitative western blotting and ELISA assay). The paroxetine data provided proof-of-concept for our strategy for further screening the APP 5'UTR target to identify novel drugs that exhibit anti-amyloid efficacy in vivo. Erythromycin and azithromycin were macrolide antibiotics that markedly changed the cleavage of the APP C-Terminal Fragment (CTF) in SH-SY5Y cells. Erythromycin provided orally to TgCRND8 mice consistently (100%) reduced brain Abeta(1-42) levels. These data demonstrated a highly statistically significant anti-amyloid trend for paroxetine, NAC and erythromycin. The potential for conducting further studies with these compounds using larger cohorts of TgCRND8 mice is discussed, particularly since erythromycin has recently been exposed to mice for a further 6 months (N=6). It will be possible to employ the chemical structures of paroxetine and erythromycin as starting points for drug design and development for AD therapeutics.
Revisiting boustrophedon coverage path planning as a generalized traveling salesman problem (2021)

ISSN:2511-1256
A Study on Power Side Channels on Mobile Devices (2015)

Power side channel is a very important category of side channels, which can be exploited to steal confidential information from a computing system by analyzing its power consumption. In this paper, we demonstrate the existence of various power side channels on popular mobile devices such as smartphones. Based on unprivileged power consumption traces, we present a list of real-world attacks that can be initiated to identify running apps, infer sensitive UIs, guess password lengths, and estimate geo-locations. These attack examples demonstrate that power consumption traces can be used as a practical side channel to gain various confidential information of mobile apps running on smartphones. Based on these power side channels, we discuss possible exploitations and present a general approach to exploit a power side channel on an Android smartphone, which demonstrates that power side channels pose imminent threats to the security and privacy of mobile users. We also discuss possible countermeasures to mitigate the threats of power side channels.
Altered Patterns of Growth Hormone Secretion in Somatostatin Knockout Mice (2006)

In summary, we have examined the physiological role of SST in the regulation of GH secretion by a gene knockout approach. The absence of SST results in elevated nadir and median GH levels in both sexes and a feminized pattern of GH-dependent gene expression in the liver of male mice. Despite the magnitude of these changes, SST−/− mice do not exhibit increased IGF-1 levels or gigantism and they retain a normal sexual dimorphism in postnatal somatic growth. A comparison of the distribution of a large set of randomly obtained GH values from mice in our study with GH data from formal pulse-analysis studies suggests that the pattern of GH secretion in both sexes of SST−/− mice is most similar to female rats. Measurement of pulsatile GH from cannulated SST-deficient mice will be necessary to confirm this hypothesis. We conclude that SST has an essential physiological role to determine the normal secretory profile of GH in both sexes of mice and specifically to masculinize GH secretion, however our data challenge the concept that a typical masculinized profile of GH pulsatility or masculinized liver is necessary for the greater somatic growth characteristics of male compared to female animals after puberty.