gebhart/patrepeval huggingface dataset:
Formulation and evaluation of wound healing activity of sophorolipid-sericin gel in wistar rats (2019)

Sericin is a useful by-product of silk processing and is resistant to oxidation and ultraviolet and can absorb and release moisture easily. Sericin has biological activities such as antibacterial, antioxidant, and tyrosinase inhibition. Sophorolipids are microbial extracellular glycolipids produced by resting cells of Candida bombicola. Sophorolipids show excellent skin compatibility and also have antibacterial property. In this study, we have developed a novel formulation consisting of sericin and sophorolipid with calcium alginate as a binding agent. Since both the ingredients are biocompatible and biodegradable, the formulation was tested for wound healing in Wistar rats. A commercial ointment povidone was used as control. The animal group, treated with sericin and sophorolipid cream, showed fast contraction, rapid closure, and healing when compared with control and commercial ointment. These observations were validated with histopathological studies where more fibroblast proliferation, angiogenesis, and keratinization were observed. This is a green, cost-effective formulation for fast wound healing.
Recombinant tumor necrosis factors mediate platelet cytotoxicity to <i>Schistosoma mansoni</i> larvae. (1988)

The involvement of platelets in the immunity directed toward the parasite Schistosoma mansoni has been demonstrated in vitro and in vivo. The killing properties of platelets were shown previously to involve specific IgE after their binding to a membrane receptor. More recently, we have demonstrated that lymphokines released by S. mansoni Ag- or lectin-stimulated CD4+/CD8-T cells were able to induce the platelet cytotoxicity. The isoelectric focusing of the T lymphocyte supernatants showed that two factors were able to stimulate the platelet killing functions. One of them was clearly identified as being IFN-gamma. The present work demonstrates that the second lymphokine was TNF. Indeed the rTNF-beta and, to a lesser extent, rTNF-alpha, induce normal platelets into killer cells for the young larvae of schistosome. Moreover, an additive effect of the TNF-alpha and IFN-gamma has been observed.
Native Human Monoclonal Antibodies with Potent Cross-Lineage Neutralization of Influenza B Viruses (2018)

Although antibodies that effectively neutralize a broad set of influenza viruses exist in the human antibody repertoire, they are rare. We used a single-cell screening technology to identify rare monoclonal antibodies (MAbs) that recognized a broad set of influenza B viruses (IBV). The screen yielded 23 MAbs with diverse germ line origins that recognized hemagglutinins (HAs) derived from influenza strains of both the Yamagata and Victoria lineages of IBV. Of the 23 MAbs, 3 exhibited low expression in a transient-transfection system, 4 were neutralizers that bound to the HA head region, 11 were stalk-binding nonneutralizers, and 5 were stalk-binding neutralizers, with 4 of these 5 having unique antibody sequences. Of these four unique stalk-binding neutralizing MAbs, all were broadly reactive and neutralizing against a panel of multiple strains spanning both IBV lineages as well as highly effective in treating lethal IBV infections in mice at both 24 and 72 h postinfection. The MAbs in this group were thermostable and bound different epitopes in the highly conserved HA stalk region. These characteristics suggest that these MAbs are suitable for consideration as candidates for clinical studies to address their effectiveness in the treatment of IBV-infected patients.
GPCR-Based Bioactive Peptide Screening Using Phage-Displayed Peptides and an Insect Cell System for Insecticide Discovery (2021)

The discovery of new insecticides improves integrated pest management (IPM), but is usually a long high-risk process with a low probability of success. For over two decades, insect neuropeptides (NPs) and their G-protein coupled receptors (GPCRs) have been considered as biological targets for insect pest control, because they are involved in almost all physiological processes associated with insect life stages. A key roadblock to success has been the question of how large volume chemical libraries can be efficiently screened for active compounds. New genomic and proteomic tools have advanced and facilitated the development of new approaches to insecticide discovery. In this study, we report a novel GPCR-based screening technology that uses millions of short peptides randomly generated by bacteriophages, and a method using an insect Sf9 cell expression system. The fire ant is a good model system, since bioactive peptides have been identified for a specific GPCR. The novel small peptides could interfere with the target GPCR-ligand functions. Therefore, we refer to this new mechanism as "receptor interference" (RECEPTORi). The GPCR-based bioactive peptide screening method offers multiple advantages. Libraries of phage-displayed peptides (~10<sup>9</sup> peptides) are inexpensive. An insect cell-based screening system rapidly leads to target specific GPCR agonists or antagonists in weeks. Delivery of bioactive peptides to target pests can be flexible, such as topical, ingestion, and plant-incorporated protectants. A variety of GPCR targets are available, thus minimizing the development of potential insecticide resistance. This report provides the first proof-of-concept for the development of novel arthropod pest management strategies using neuropeptides, and GPCRs.
Cloning of the<i>Alcaligenes latus</i>Polyhydroxyalkanoate Biosynthesis Genes and Use of These Genes for Enhanced Production of Poly(3-hydroxybutyrate) in<i>Escherichia coli</i> (1998)

Polyhydroxyalkanoates (PHAs) are microbial polyesters that can be used as completely biodegradable polymers, but the high production cost prevents their use in a wide range of applications. Recombinant Escherichia coli strains harboring the Ralstonia eutropha PHA biosynthesis genes have been reported to have several advantages as PHA producers compared with wild-type PHA-producing bacteria. However, the PHA productivity (amount of PHA produced per unit volume per unit time) obtained with these recombinant E. coli strains has been lower than that obtained with the wild-type bacterium Alcaligenes latus. To endow the potentially superior PHA biosynthetic machinery to E. coli, we cloned the PHA biosynthesis genes from A. latus. The three PHA biosynthesis genes formed an operon with the order PHA synthase, beta-ketothiolase, and reductase genes and were constitutively expressed from the natural promoter in E. coli. Recombinant E. coli strains harboring the A. latus PHA biosynthesis genes accumulated poly(3-hydroxybutyrate) (PHB), a model PHA product, more efficiently than those harboring the R. eutropha genes. With a pH-stat fed-batch culture of recombinant E. coli harboring a stable plasmid containing the A. latus PHA biosynthesis genes, final cell and PHB concentrations of 194.1 and 141.6 g/liter, respectively, were obtained, resulting in a high productivity of 4.63 g of PHB/liter/h. This improvement should allow recombinant E. coli to be used for the production of PHB with a high level of economic competitiveness.
Improving Gated Cardiac Scanning Using Limited-Angle Reconstruction Technique (1983)

Selected phases of the cardiac cycle can be imaged by the method of gated cardiac scanning using whole body scanners. However, since there is no synchronization between the random heart beats and the CT machine, the output will have gaps in the angular coverage of each scan. The missing projections in these gaps result in artifacts in the reconstructed images. In this paper the technique of limited-angle reconstruction is employed to fill in the missing projections to remove the artifacts. The object is transformed back and forth between the object space and the projection space, being corrected in each step by the constraints of the finite spatial extent and of the upper and lower bounds of the object in the object space, and the known projections in the projection space. It is found that by using the proposed algorithm the rms errors of the limited-angle images are reduced to a level comparable to those of the complete-angle images. The same iteration algorithm can also be used to reduce the fan beam scanning angle from 360 degree to 180 degree without sacrificing much image quality.
Dual-Stage Cascaded Frequency Offset Estimation for Digital Coherent Receivers (2010)

Frequency offset estimation is a key function in carrier phase recovery to enable digital coherent reception. We propose a novel, feed-forward, dual-stage frequency offset estimator, and demonstrate its performance through both simulation and experiment. Our simulation results show that the estimation range can be as large as ± 9 GHz for a 10.7-GBaud polarization-multiplexed (PolMux) quadrature phase-shift keying (QPSK) system. In addition, the widest range of ±0.5 times the system symbol rate is achieved for the first time in an experimental 42.8-Gb/s coherent PolMux QPSK receiver.
A high efficiency cloning and expression system for proteomic analysis (2006)

The recent description of the complete genomes of the two most pathogenic species of Brucella opens the way for genome-based analysis of the antigenicity of their proteins. In the present report, we describe a bench-level high-efficiency cloning and expression system (HECES) that allow expression of large numbers of Brucella proteins based on genomic sequence information. Purified proteins are produced with high efficiency in a microarray format conducive to analysis of their sero-reactivity against serum from immunized animals. This method is applicable at either small or large scale of protein processing. While it does not require robotics, the format is amenable to robotic implementation for all aspects of the process and subsequent analysis of protein characteristics. This method will allow selection of new reagents for diagnosis of brucellosis and development of vaccine against Brucella, an important zoonotic disease and biothreat agent.
Oxidation–reduction assisted exfoliation of LiCoO<sub>2</sub> into nanosheets and reassembly into functional Li-ion battery cathodes (2016)

A new method to exfoliate LiCoO<sub>2</sub> into nanosheets is presented and the structural and electrochemical properties of particles obtained from reassembled nanosheets are investigated.
A method for patient-specific absorbed dose estimation for internal beta emitters. (1999)

The purpose of the study was to determine a technique for estimating patient-specific absorbed radiation doses in radioimmunotherapy and other internal emitter therapies. Beta Radiation sources were considered, with 90Y being the radionuclide of primary interest. Organ uptake of activity was determined using a merged set of computed tomography and planar nuclear images. Estimation of local absorbed dose was accomplished using a voxel source kernel. Voxel size was 0.2 x 0.2 x 0.5 cm; dimensions were from the digital resolution of the nuclear and computed tomography data sets. Dose-volume histograms were also obtained due to the voxel nature of the estimations. Organ dose estimates were made for two patients receiving the chimeric anticarcinoembryonic antigen antibody cT84.66. Considerable variation was observed when comparing the voxel kernel results with medical internal radiation dosimetry values obtained via the MIRDOSE3 program. Primary uncertainty in the organ dose estimates was determined to be due to the variation in organ mass. By correcting the S values in that program by the organ mass ratio, we found generally good agreement between our method and MIRDOSE3. We conclude that patient-specific absorbed doses can be estimated for 90Y-labeled antibodies.
A Nd^3+-doped cw fiber laser using all-fiber reflectors (1987)

We demonstrate a novel all-fiber resonant optical cavity which uses two-fiber reflectors, each formed by a single loop of fiber between the output ports of a fiber directional coupler. The reflectivities of the fiber mirrors are each determined by the coupling ratio and the insertion loss of the fused couplers. When the cavity is formed in this way using a continuous length of Nd(3+)-doped fiber and pumped using a GaAs laser diode, lasing occurs at a wavelength of 1064 nm. Both theoretical and practical descriptions of the device are given.
Isolation of a replication region of a large lactococcal plasmid and use in cloning of a nisin resistance determinant (1990)

The replication region of a 28-kilobase-pair (kbp) cryptic plasmid from Lactococcus lactis subsp. lactis biovar diacetylactis SSD207 was cloned in L. lactis subsp. lactis MG1614 by using the chloramphenicol resistance gene from the streptococcal plasmid pGB301 as a selectable marker. The resulting 8.1-kbp plasmid, designated pVS34, was characterized further with respect to host range, potential cloning sites, and location of replication gene(s). In addition to lactococci, pVS34 transformed Lactobacillus plantarum and, at a very low frequency, Staphylococcus aureus but not Escherichia coli or Bacillus subtilis. The 4.1-kbp ClaI fragment representing lactococcal DNA in pVS34 contained unique restriction sites for HindIII, EcoRI, XhoII, and HpaII, of which the last three could be used for molecular cloning. A region necessary for replication was located within a 2.5-kbp fragment flanked by the EcoRI and ClaI restriction sites. A 3.8-kbp EcoRI fragment derived from a nisin resistance plasmid, pSF01, was cloned into the EcoRI site of pVS34 to obtain a nisin-chloramphenicol double-resistance plasmid, pVS39. From this plasmid, the streptococcal chloramphenicol resistance region was subsequently eliminated. The resulting plasmid, pVS40, contains only lactococcal DNA. Potential uses for this type of a nisin resistance plasmid are discussed.
MicroRNA-455-3p as a potential peripheral biomarker for Alzheimer’s disease (2017)

The purpose of our study was to identify microRNAs (miRNAs) as early detectable peripheral biomarkers in Alzheimer's disease (AD). To achieve our objective, we assessed miRNAs in serum samples from AD patients and Mild cognitive impairment (MCI) subjects relative to healthy controls. We used Affymetrix microarray analysis and validated differentially expressed miRNAs using qRT-PCR. We further validated miRNA data using AD postmortem brains, amyloid precursor protein transgenic mice and AD cell lines. We identified a gradual upregulation of four miRNAs: miR-455-3p, miR-4668-5p, miR-3613-3p and miR-4674. A fifth miRNA, mir-6722, was down-regulated in persons with AD and mild cognitive impairment compared with controls. Validation analysis by qRT-PCR showed significant upregulation of only miR-455-3p (P = 0.007) and miR-4668-5p (P = 0.016) in AD patients compared with healthy controls. Furthermore, qRT-PCR analysis of the AD postmortem brains with different Braak stages also showed upregulation of miR-455-3p (P = 0.016). However, receiver operating characteristic curves (ROC) curve analysis revealed a significant area under curve (AUC) value only for miR-455-3p in the serum (AUROC = 0.79; P = 0.015) and brains (AUROC = 0.86; P = 0.016) of AD patients. Expression analysis of amyloid precursor protein transgenic mice also revealed high level of mmu-miR-455-3p (P = 0.004) in the cerebral cortex (AD-affected) region of brain and low in the non-affected area, i.e. cerebellum. Furthermore, human and mouse neuroblastoma cells treated with the amyloid-β(1-42) peptide also showed a similarly higher expression of miR-455-3p. Functional analysis of differentially expressed miRNAs via the miR-path indicated that miR-455-3p was associated in the regulation of several biological pathways. Genes associated with these pathways were found to have a crucial role in AD pathogenesis. An increase in miR-455-3p expression found in AD patients and Aβ pathologies unveiled its biomarker characteristics and a precise role in AD pathogenesis.
A power flow transfer optimization strategy for important transmission channels based on lightning early warning (2021)

With the substantial increase in global thunderstorm activities, the hazards of thunder and lightning to the security of the power grid are increasingly not to be ignored. Due to the large scale of the power grid, the small probability event that the trip of inter-provincial tie line of the same tower due to lightning strikes may still trigger the accident cascade effect of the power grid, which seriously threatens the power supply reliability of the power grid. This paper proposes an active lightning protection measure based on lightning warning which adjusts the power flow of transmission lines that are threatened by lightning strikes by controlling the output of key power sources. And it is different from the traditional static lightning protection measures such as erecting lightning shield lines. This paper also establishes a power flow transfer optimization model that takes into account system constraints such as generator output. Finally, taking the actual large-scale system of Central China Power Grid as an example, the implementation process of the proposed lightning protection strategy is simulated and analyzed, and the research results have reference value for the lightning protection of important power transmission channels.
Quantum Computing with Majorana Kramers Pairs (2018)

We propose a universal gate set acting on a qubit formed by the degenerate ground states of a Coulomb-blockaded time-reversal invariant topological superconductor island with spatially separated Majorana Kramers pairs: the "Majorana Kramers Qubit". All gate operations are implemented by coupling the Majorana Kramers pairs to conventional superconducting leads. Interestingly, in such an all-superconducting device, the energy gap of the leads provides another layer of protection from quasiparticle poisoning independent of the island charging energy. Moreover, the absence of strong magnetic fields - which typically reduce the superconducting gap size of the island - suggests a unique robustness of our qubit to quasiparticle poisoning due to thermal excitations. Consequently, the Majorana Kramers Qubit should benefit from prolonged coherence times and may provide an alternative route to a Majorana-based quantum computer.
1-(Fluorobenzyl)-4-amino-1H-1,2,3-triazolo[4,5-c]pyridines: Synthesis and Anticonvulsant Activity (1995)

A series of (fluorobenzyl)triazolo[4,5-c]pyridines was synthesized and tested for activity against maximal electroshock-induced seizures in rodents. The most promising compound, 14 (BW 534U87), which is a carbon-nitrogen isoster of a purine anticonvulsant, has a profile in rodents that suggests 14 will be free of emesis and useful in the treatment of seizure disorders for which phenytoin is presently indicated.
Partially Coherent Phase Recovery by Kalman Filtering (2013)

We demonstrate a Kalman filtering method to recover the phase of a thin object illuminated by partially coherent light. Our method is fast, efficient, robust to noise, and able to handle arbitrary source shapes when used in a microscope with Kohler illumination.
Submicron Si trench profiling with an electron-beam fabricated atomic force microscope tip (1991)

The atomic force microscope (AFM) has the potential for profiling on a nanometer scale. However, for the AFM to be useful as a reliable and accurate metrology tool, the atomic force sensor used (i.e., cantilever and sensor tip) should be highly reproducible in the fabrication process and the tip shape used should be known with high accuracy. Moreover, for profiling and critical dimension measurement of device trenches with submicron openings and trench depth of 1 μm or more, nanometer-scale tip shapes with high aspect ratio will be required. In order to meet the above requirement, a novel high-resolution direct electron-beam deposition process has been developed in which a sharp tip is grown onto an existing Si cantilever structure. In the example to be discussed, sharp tips have been grown directly on the integrated tip of a micromachined Si cantilever by decomposition of a dimethyl-gold organometallic complex. The direct e-beam deposition process provides a unique capability for the controllable fabrication of high aspect ratio, nanometer-scale tip structures. Typical dimensions of the AFM tips achieved are 0.1 μm in column diameter, 1–4 μm high, and 15 nm in tip radius. Recent feasibility experiments of using these e-beam tips in the laser force microscope have demonstrated for the first time that this nano-scale tip can be used for mapping 1-μm-deep Si trenches. Submicron trenches with openings as small as 0.36 μm have been successfully profiled. In this paper, various issues on tip fabrication, deep trench profiling and comparision of AFM and corresponding scanning electron microscopy measurements will be discussed.
PD-L1 Expression and Survival among Patients with Advanced Non–Small Cell Lung Cancer Treated with Chemotherapy (2016)

In concordance with previous studies, we found PD-L1 measured by immunohistochemistry to be frequently expressed in patients with advanced NSCLC. However, PD-L1 expression is not a strong prognostic marker in patients with advanced NSCLC treated with chemotherapy.
Simultaneous covalent and noncovalent hybrid polymerizations (2016)

Covalent and supramolecular polymers are two distinct forms of soft matter, composed of long chains of covalently and noncovalently linked structural units, respectively. We report a hybrid system formed by simultaneous covalent and supramolecular polymerizations of monomers. The process yields cylindrical fibers of uniform diameter that contain covalent and supramolecular compartments, a morphology not observed when the two polymers are formed independently. The covalent polymer has a rigid aromatic imine backbone with helicoidal conformation, and its alkylated peptide side chains are structurally identical to the monomer molecules of supramolecular polymers. In the hybrid system, covalent chains grow to higher average molar mass relative to chains formed via the same polymerization in the absence of a supramolecular compartment. The supramolecular compartments can be reversibly removed and re-formed to reconstitute the hybrid structure, suggesting soft materials with novel delivery or repair functions.
Linear polarization difference imaging and its potential applications (2009)

We demonstrate a novel linear polarization imaging technique and its potential application in dermatology. This technique records a series of images corresponding to different combinations of illumination and detection polarization and calculates intensity differences between orthogonal detection polarizations pixel by pixel. Fitting the polarization difference data to an analytical expression of the incident and detection polarization angles results in two new parameters, G and (phi3)/2. It is shown that G is strongly correlated to the order of alignment of the fibrous structure in the sample, and (phi3)/2 represents the angle of orientation of the fibers. Preliminary clinical testing implies that this method may be applied for medical diagnosis of skin diseases.
Hybrid distributed acoustic-temperature sensing using a few-mode fiber (2020)

Optical fiber distributed acoustic sensor (DAS) and distributed temperature sensor (DTS) are considerably desirable for many important applications including oil and gas industry. Simultaneous measurements of vibration and temperature will exclude the need for two separate DAS and DTS systems, reduce overall cost, and ensure continuous real-time monitoring of these two important sensing parameters. We here devise a hybrid DAS-DTS system using a few-mode fiber (FMF). Although the system requirements for DAS and DTS are quite different, FMF is considered an ideal compromise to satisfy the requirements of the two systems.