gebhart/patrepeval huggingface dataset:
Single-step affinity purification of toxic and non-toxic proteins on a fluidics platform (2005)

Single-step fusion-based affinity purification of proteins with pH-controllable linkers was carried out in a fluidic device. The linkers were previously derived from self-splicing protein elements called inteins. Two different linkers were generated to solve two distinct separation problems: one for rapid single-step affinity purification of a wide range of proteins, and the other specifically for the purification of cytotoxic proteins. Scale-down factors of 185 resulted in separations in a 27 microl bed-volume. A rotating CD format was chosen because of its simplicity in effecting fluid movement through centrifugal force without the complications associated with electro-osmosis and other pumping methods. The design and fabrication of the fluidic device and the protein purification process are described. This work, which demonstrates the purification of active proteins by two distinct fluidic separations, is widely applicable to small-scale massively parallel proteomic separations.
진해만 해수의 부영양화와 클로로필 분포 (1975)

1) 클로로필a 量은 馬山灣이 4.10-25.16㎎/㎥ 범위에 平均 13.27 ㎎/㎥로 가장 높은 分布를 보였으며 鎭海化事工場이 位置한 行岩灣은 0.52-8.27㎎/㎥에 平均 4.29 ㎎/㎥로 인근 洛東江 浦口의 3.12-5.73 ㎎/㎥에 平均4.46㎎/㎥보다 낮았으며 굴양식장이 많은 巨濟灣이 0.65-5.00㎎/㎥에 平均 2.23㎎/㎥로 가장 낮은 分布를 보였다.
2) 營養鹽類의 N/P값이 12以上되는 馬山灣과 洛東江 河口에서는 營養鹽量의 增加와 클로로필a量의 增加는 比例하고 있었으나 鎭海化學 工場廢水의 영향이로 N/P값이 2 以下로 낮은 行岩灣 및 鎭海灣 內海域에서는 營養鹽類量의 增加와 클로로필a量과는 一定한 相關關係가 없었고 燐酸鹽量이 過度하게 많은 行岩灣에서는 오히려 클로로필a量이 더 적은 結果을 보였다.
EchoWear: Smartwatch Technology for Voice and Speech Treatments of Patients with Parkinson's Disease (2015)

About 90 percent of people with Parkinson's disease (PD) experience decreased functional communication due to the presence of voice and speech disorders associated with dysarthria that can be characterized by monotony of pitch (or fundamental frequency), reduced loudness, irregular rate of speech, imprecise consonants, and changes in voice quality. Speech-language pathologists (SLPs) work with patients with PD to improve speech intelligibility using various intensive in-clinic speech treatments. SLPs also prescribe home exercises to enhance generalization of speech strategies outside of the treatment room. Even though speech therapies are found to be highly effective in improving vocal loudness and speech quality, patients with PD find it difficult to follow the prescribed exercise regimes outside the clinic and to continue exercises once the treatment is completed. SLPs need techniques to monitor compliance and accuracy of their patients exercises at home and in ecologically valid communication situations. We have designed EchoWear, a smartwatch-based system, to remotely monitor speech and voice exercises as prescribed by SLPs. We conducted a study of 6 individuals; three with PD and three healthy controls. To assess the performance of EchoWear technology compared with high quality audio equipment obtained in a speech laboratory. Our preliminary analysis shows promising outcomes for using EchoWear in speech therapies for people with PD. Keywords: Dysarthria; knowledge-based speech processing; Parkinson's disease; smartwatch; speech therapy; wearable system.
Phase III Study to Evaluate Temsirolimus Compared With Investigator's Choice Therapy for the Treatment of Relapsed or Refractory Mantle Cell Lymphoma (2009)

Temsirolimus 175 mg weekly for 3 weeks followed by 75 mg weekly significantly improved PFS and objective response rate compared with investigator's choice therapy in patients with relapsed or refractory MCL.
Phase <scp>III</scp> results of Boceprevir in treatment naïve patients with chronic hepatitis C genotype 1 (2011)

Triple therapy of Boceprevir in combination with PEG-IFN 2b/RBV is more effective than SOC alone. RGT is possible without reducing the SVR rates. Management of anaemia has to be considered.
A category-level 3-D object dataset: Putting the Kinect to work (2011)

Recent proliferation of a cheap but quality depth sensor, the Microsoft Kinect, has brought the need for a challenging category-level 3D object detection dataset to the fore. We review current 3D datasets and find them lacking in variation of scenes, categories, instances, and viewpoints. Here we present our dataset of color and depth image pairs, gathered in real domestic and office environments. It currently includes over 50 classes, with more images added continuously by a crowd-sourced collection effort. We establish baseline performance in a PASCAL VOC-style detection task, and suggest two ways that inferred world size of the object may be used to improve detection. The dataset and annotations can be downloaded at http://www.kinectdata.com.
Going Viral: The Epidemiological Strategy of Referral Marketing (2018)

By now, Internet word-of-mouth marketing has established its importance for almost every kind of products. When word-of-mouth spread of a marketing campaign goes viral, the success of its strategy has been proved to be path-breaking for the firms. The current study is designed to extend knowledge about customer response to these campaigns, and is focused on the motivations that lead them to different responses. Primary goal of this study is to investigate the reasons that drive this dynamics and to generate a justified theoretical framework of diffusion dynamics of viral marketing campaigns. A rigorous analysis of data obtained through a questionnaire-based survey helped us to understand how people, who are from different age group, sex and locations, define and evaluate the encounter with a referral campaign online, in similar and unique logical ways. The key finding was a conceptual framework of customer motivation which in turn dictates the dynamics of the campaign. We have identified important themes related to customer motives, like significance of: incentives and ease, inherent forgetting, reminders from peers compared to company's remarketing mails, trust and brand value etc. Drawing an analogy with differential equation-based models of infectious disease spread, this paper further provides some initial evidence that participation in viral marketing campaigns has several consumer related dynamical factors which can be incorporated in an epidemiological model for mathematical treatment, to indicate key operational factors in ensuring an effective spread of the marketing campaign.
Modification of flexographic printing plate’s surface properties by variation of UVC finishing (2013)

Flexography is a printing technique mostly used in packaging printing. In the beginning, used mostly for printing on the corrugated board, this technique has developed and is applied today in printing on various packaging materials and printed electronics. Many parameters influence the quality of the final product in flexography: photopolymer material used for the printing plate production, quality of the file adjustment, type of the anilox roller, type of the base placed under the printing plate to regulate the pressure, properties of the printing ink and printing substrate, and a set of parameters associated with the printing plate production. The printing plate making process includes three exposures to UV light, and finishing with exposure to UVC wavelength. Finishing has to be performed in order to terminate the photopolymeriazation process. During the transfer of the printing ink from the printing plate to the printing substrate, surface properties of the printing plate highly influence the quality of the imprint. The aim of this paper was to determine the influence of flexographic CtP printing plate’s UVC finishing on its surface properties by means of contact angle, surface free energy and EDS analysis. Standard UVC finishing for this type of printing plate equals 4 minutes of irradiation, with central wavelength of 256 nm, 18 mW/cm2. In order to define the influence of different duration of UVC finishing on the surface characteristics of the printing plate, samples used in this research varied from UVC finishing duration of 1 minute to the extreme of 14 minutes. Contact angle was measured by means of Sessile drop method, and surface free energy was calculated applying OWRK method. Results have indicated that UVC finishing influences printing plate’s physico-chemical surface characteristics by changing the contact angle and the surface free energy parameters. This could result with negative influence on the process of the printing ink transfer from the printing plate to the printing substrate. Measurements indicated that prolonged UVC radiation results with lower contact angle, primarily of the polar probe liquid, with final outcome of increase of the total surface free energy. EDS analysis showed that the changes in contact angles of probe liquids and surface free energy could be caused by the increase of oxygen concentration in the surface layer of the printing plate. Therefore, duration and intensity of UVC finishing must be strictly adjusted and regularly monitored in order to avoid unwanted changes of the flexographic printing plate‘s surface properties, consequently its behaviour in the printing process.
Scientific Opinion on the safety and efficacy of the use of amino acids (chemical group 34) when used as flavourings for all animal species (2014)

Chemical group (CG) 34 comprises amino acids, of which 20 are currently authorised for use as flavours in food. The FEEDAP Panel was unable to perform an assessment of D,L-valine because of the lack of data on its purity. The Panel cannot conclude on the use of these compounds in water for drinking. In the absence of any information on the microbial strains or substrates used for the production of the additives, and with little information on the manufacturing process, the Panel cannot ascertain whether the manufacturing process introduces any safety concerns. The conclusions on target animals and consumer safety apply only to the compounds per se, and can be extrapolated only to feed additives containing these compounds when obtained by chemical synthesis or protein hydrolysis and to those produced by fermentation which have already undergone a strain-specific safety assessment by EFSA. The 19 compounds under assessment in CG 34 are safe at the proposed use level of 25 mg/kg feed for all animal species. The FEEDAP Panel considers the use of glycine at 20 g/kg in food for cats and dogs as safe. The safety of the proposed use of beta-alanine in pet food at 20 g/kg was not substantiated. Use of these compounds does not give rise to concern for consumer safety. The Panel considers it prudent to treat all 19 compounds as irritant to skin and eyes, skin sensitisers and hazardous by inhalation. They do not give rise to any concern for the safety of the environment. No further demonstration of their efficacy is necessary when used at concentrations up to 25 mg/kg complete feed. There is some evidence that high concentrations (20 g/kg in cat and dog food) of glycine may influence the food preference of cats and dogs. Comparable evidence for beta-alanine is not available.
Differentiation of PC12 cells in response to a cAMP analogue is accompanied by sustained activation of mitogen‐activated protein kinase (1994)

It has been proposed previously that the sustained activation of mitogen-activated protein kinase may be necessary for the differentiation of PC12 cells. Differentiation of PC12 cells is induced by many extracellular agonists including nerve growth factor (NGF) and cyclicAMP analogues, but not epidermal growth factor (EGF), insulin or phorbol esters. Our results demonstrate that: (i) 8-(4-chlorophenylthio)-cyclicAMP (CPT-cAMP) activates MAP kinase; this raises the possibility that the MAP kinase pathway may be activated by agents that act through adenylate cyclase; (ii) NGF and CPT-cAMP as well as phorbol esters promote sustained activation of MAP kinase. This suggests that while sustained MAP kinase activation may be associated with differentiation it may not be sufficient, and that other as yet unidentified parallel pathways may be involved.
Thin-shell textile-reinforced concrete floors for sustainable buildings (2017)

Steel-reinforced concrete, cast in flat prismatic forms, dominates multi-storey building construction around the world. Despite the fluidity of the material, opportunities to create efficient geometries through manipulation of form are habitually overlooked, resulting in inefficient cracked sections, high steel requirements and large carbon footprints. This project brings together modern developments in computational design, materials and construction to propose a novel thin-shell concrete flooring system for multi-storey buildings, creating a low embodied energy and lightweight alternative to traditional reinforced concrete flat slabs. In this investigation, the performance of various shell geometries are compared using finite element analysis. A functional design is produced and found to offer reductions of 62% in embodied energy and 64% in weight compared to an equivalent flat slab.
Regular Texture Analysis as Statistical Model Selection (2008)

An approach to the analysis of images of regular texture is proposed in which lattice hypotheses are used to define statistical models. These models are then compared in terms of their ability to explain the image. A method based on this approach is described in which lattice hypotheses are generated using analysis of peaks in the image autocorrelation function, statistical models are based on Gaussian or Gaussian mixture clusters, and model comparison is performed using the marginal likelihood as approximated by the Bayes Information Criterion (BIC). Experiments on public domain regular texture images and a commercial textile image archive demonstrate substantially improved accuracy compared to two competing methods. The method is also used for classification of texture images as regular or irregular. An application to thumbnail image extraction is discussed.
Subsequent Evolution of Fertility after Surgical Treatment of Delivery Haemorrhage using the B-Lynch Technique at the Chumefje in Libreville (Gabon) (2023)

Introduction: B-lynch suture is effective in controlling delivery haemorrhage due to uterine atony that is resistant to medical and obstetric measures. However, there are still concerns about the potential complications resulting from poor uterotubal perfusion. Patients and Methods: This was a retrospective descriptive study of patients who underwent a B-Lynch technique at the Centre Hospitalier Universitaire Mère-Enfant Fondation Jeanne Ebori (CHUMEFJE) from January 1, 2019 to June 30, 2021. Results: The mean age was 28.9 ± 3.7 years. The B-Lynch technique stopped haemorrhage in 20 (83.3%) patients. Immediate or short-term post-operative complications were dominated by haemorrhagic shock in 5 (20.8%) patients. No deaths were recorded. Return of childbirth was observed in 18 (90%) patients. Eight (40%) patients became pregnant spontaneously. None presented major complications during pregnancy. Conclusion: The B-lynch technique is effective in cases of delivery haemorrhage caused by uterine atony that is resistant to medical and obstetric measures. It reduces maternal mortality while generally preserving subsequent fertility
DOT1L safeguards cartilage homeostasis and protects against osteoarthritis (2017)

Abstract Osteoarthritis is the most prevalent and crippling joint disease, and lacks curative treatment, as the underlying molecular basis is unclear. Here, we show that DOT1L, an enzyme involved in histone methylation, is a master protector of cartilage health. Loss of DOT1L disrupts the molecular signature of healthy chondrocytes in vitro and causes osteoarthritis in mice. Mechanistically, the protective function of DOT1L is attributable to inhibition of Wnt signalling, a pathway that when hyper-activated can lead to joint disease. Unexpectedly, DOT1L suppresses Wnt signalling by inhibiting the activity of sirtuin-1 (SIRT1), an important regulator of gene transcription. Inhibition of SIRT1 protects against osteoarthritis triggered by loss of DOT1L activity. Modulating the DOT1L network might therefore be a therapeutic approach to protect the cartilage against osteoarthritis.
Baicalein, an α-Glucosidase Inhibitor from <i>Scutellaria baicalensis</i> (1998)

Methanol extracts of Scutellaria baicalensis, Rheum officinale, and Paeonia suffruticosa showed potent inhibitory activity against rat intestinal sucrase. The active principles were identified as baicalein from the first and methyl gallate from the last two plants. In addition to its activity against the rat enzyme, baicalein also inhibited human intestinal sucrase expressed in Caco-2 cells.
Carbon sequestration via wood burial (2008)

To mitigate global climate change, a portfolio of strategies will be needed to keep the atmospheric CO2 concentration below a dangerous level. Here a carbon sequestration strategy is proposed in which certain dead or live trees are harvested via collection or selective cutting, then buried in trenches or stowed away in above-ground shelters. The largely anaerobic condition under a sufficiently thick layer of soil will prevent the decomposition of the buried wood. Because a large flux of CO2 is constantly being assimilated into the world's forests via photosynthesis, cutting off its return pathway to the atmosphere forms an effective carbon sink.It is estimated that a sustainable long-term carbon sequestration potential for wood burial is 10 +/- 5 GtC y-1, and currently about 65 GtC is on the world's forest floors in the form of coarse woody debris suitable for burial. The potential is largest in tropical forests (4.2 GtC y-1), followed by temperate (3.7 GtC y-1) and boreal forests (2.1 GtC y-1). Burying wood has other benefits including minimizing CO2 source from deforestation, extending the lifetime of reforestation carbon sink, and reducing fire danger. There are possible environmental impacts such as nutrient lock-up which nevertheless appears manageable, but other concerns and factors will likely set a limit so that only part of the full potential can be realized.Based on data from North American logging industry, the cost for wood burial is estimated to be $14/tCO2($50/tC), lower than the typical cost for power plant CO2 capture with geological storage. The cost for carbon sequestration with wood burial is low because CO2 is removed from the atmosphere by the natural process of photosynthesis at little cost. The technique is low tech, distributed, easy to monitor, safe, and reversible, thus an attractive option for large-scale implementation in a world-wide carbon market.
Rheometric Study of the Gelation of Chitosan in Aqueous Solution without Cross-Linking Agent (2005)

A new process of formation of chitosan physical hydrogels in aqueous solution, without any organic solvent or cross-linking additive, was studied. The three conditions required for the physical gelation were an initial polymer concentration over C*, a critical value of the balance between hydrophilic and hydrophobic interactions, and a physicochemical perturbation responsible for a bidimensional percolating mechanism. The time necessary to reach the gel point was determined by rheometry, and gelations were compared according to different initial conditions. Thus, we investigated the influence of the polymer concentration and the degree of acetylation (DA) of chitosan on gelation. The number of junctions per unit volume at the gel point varied with the initial polymer concentration, i.e., the initial number of chain entanglements per unit volume or the number of gel precursors. The time to reach the gel point decreased with both higher DAs and concentrations. For a chitosan of DA = 36.7%, a second critical initial concentration close to 1.8% (w/w) was observed. Above this concentration, the decrease of the time to reach the gel point was higher and fewer additional junctions had to be formed to induce gelation. To optimize these physical hydrogels, to be used for cartilage regeneration, their final rheological properties were studied as a function of their degree of acetylation and their polymer concentration. Our results allowed us to define the most appropriate gel for the targeted application corresponding to a final concentration of chitosan in the gel of near 1.5% (w/w) and a DA close to 40%.
Mass Spectrometric Analysis of Neutral and Anionic N-Glycans from a <i>Dictyostelium discoideum</i> Model for Human Congenital Disorder of Glycosylation CDG IL (2013)

The HL241 mutant strain of the cellular slime mold Dictyostelium discoideum is a potential model for human congenital disorder of glycosylation type IL (ALG9-CDG) and has been previously predicted to possess a lower degree of modification of its N-glycans with anionic moieties than the parental wild-type. In this study, we first showed that this strain has a premature stop codon in its alg9 mannosyltransferase gene compatible with the occurrence of truncated N-glycans. These were subject to an optimized analytical workflow, considering that the mass spectrometry of acidic glycans often presents challenges due to neutral loss and suppression effects. Therefore, the protein-bound N-glycans were first fractionated, after serial enzymatic release, by solid phase extraction. Then primarily single glycan species were isolated by mixed hydrophilic-interaction/anion-exchange or reversed-phase HPLC and analyzed using chemical and enzymatic treatments and MS/MS. We show that protein-linked N-glycans of the mutant are of reduced size as compared to those of wild-type AX3, but still contain core α1,3-fucose, intersecting N-acetylglucosamine, bisecting N-acetylglucosamine, methylphosphate, phosphate, and sulfate residues. We observe that a single N-glycan can carry up to four of these six possible modifications. Due to the improved analytical procedures, we reveal fuller details regarding the N-glycomic potential of this fascinating model organism.
Depletion of white adipocyte progenitors induces beige adipocyte differentiation and suppresses obesity development (2014)

Overgrowth of white adipose tissue (WAT) in obesity occurs as a result of adipocyte hypertrophy and hyperplasia. Expansion and renewal of adipocytes relies on proliferation and differentiation of white adipocyte progenitors (WAP); however, the requirement of WAP for obesity development has not been proven. Here, we investigate whether depletion of WAP can be used to prevent WAT expansion. We test this approach by using a hunter-killer peptide designed to induce apoptosis selectively in WAP. We show that targeted WAP cytoablation results in a long-term WAT growth suppression despite increased caloric intake in a mouse diet-induced obesity model. Our data indicate that WAP depletion results in a compensatory population of adipose tissue with beige adipocytes. Consistent with reported thermogenic capacity of beige adipose tissue, WAP-depleted mice display increased energy expenditure. We conclude that targeting of white adipocyte progenitors could be developed as a strategy to sustained modulation of WAT metabolic activity.