gebhart/patrepeval huggingface dataset:
Identification of CD19 and CD20 Peptides for Induction of Antigen-Specific CTLs against B-Cell Malignancies (2005)

The purpose of these studies was to develop immunogenic peptides derived from the CD19 and CD20 self-antigens for the induction of antigen-specific CTLs against B-cell malignancies. A total of seven peptides were designed and examined for their HLA-A2.1 affinity and immunogenicity. Of these peptides, we identified two highly immunogenic HLA-A2.1-specific peptides, CD19(150-158) (KLMSPKLYV) and CD20(188-196) (SLFLGILSV), which were capable of inducing peptide-specific CTLs. The CTLs displayed HLA-A2.1-restricted and antigen-specific cytotoxicity against Burkitt's lymphoma, chronic B cell leukemia, and multiple myeloma cell lines. The CD19 or CD20 peptide-specific CTL cytotoxicity was confirmed using HLA-A2.1(+) T2 cells presenting the appropriate peptide. No cytotoxic activity was observed against T2 cells presenting the irrelevant MAGE-3 peptide or T2 cells alone. In addition, the CTLs displayed a significant (P < 0.05) increase in cell proliferation and IFN-gamma secretion (>830 ng/mL) following restimulation with HLA-A2.1(+)/CD19(+)/CD20(+) tumor cells. The CTLs also displayed a distinct phenotype consisting of a high percentage of CD69(+)/CD45RO(+) and a low percentage of CD45RA(+)/CCR7(+) CD4(+) or CD8(+) T cells characteristic of effector memory cell population. Cyclic guanosine 3',5'-monophosphate culture conditions using serum-free AIM-V medium containing human AB serum, recombinant human interleukin 2 (Proleukin) and CD3/CD28 Dynabeads were developed resulting in a 35-fold expansion of CD20 peptide-specific CTLs. The expanded CD20-CTLs retained their cytotoxic activity (28-49%) against the Burkitt's lymphoma cell line. In conclusion, we report here on the identification of novel immunogenic CD19(150-158) (KLMSPKLYV) and CD20(188-196) (SLFLGILSV) peptides that have immunotherapeutic potentials as peptide vaccines or targeted T-cell therapies for treating B-cell malignancies.
Cytokinesis in Animal Cells (2012)

Cytokinesis, the final step in cell division, partitions the contents of a single cell into two. In animal cells, cytokinesis occurs through cortical remodeling orchestrated by the anaphase spindle. Cytokinesis relies on a tight interplay between signaling and cellular mechanics and has attracted the attention of both biologists and physicists for more than a century. In this review, we provide an overview of four topics in animal cell cytokinesis: (a) signaling between the anaphase spindle and cortex, (b) the mechanics of cortical remodeling, (c) abscission, and (d) regulation of cytokinesis by the cell cycle machinery. We report on recent progress in these areas and highlight some of the outstanding questions that these findings bring into focus.
The Limitations of Energy Density for Electrochemical Capacitors (1997)

Electrochemical capacitors can be divided into two types depending on whether the salt concentration in the electrolyte changes during charging and discharging. In the first type of capacitor, such as double‐layer capacitors, the salt concentration in the electrolyte reduces during the charging of the capacitor. The maximum energy density of this type of capacitor will depend not only on the specific capacitance and the operating voltage, but also on the salt concentration of the electrolyte. In this paper, a formula describing the dependence of energy density on specific capacitance, operating voltage, and salt concentration is given based on the optimized weight (or volume) ratio of the electrode material and the electrolyte. It shows that for electrochemical capacitors using nonaqueous electrolytes, the maximum energy density of the capacitor will be limited mainly by the low salt concentrations of the electrolyte. The relationship between the energy density and the mass density of the electrode is also given. The optimum mass density of the electrode can be obtained based on the value of the theoretical energy density for capacitors with different electrolytes. In the second type of capacitor, such as pseudocapacitors with metal oxide electrodes, the salt concentration in the electrolyte remains constant during charging and discharging. The maximum energy density of this type of capacitor will be limited mainly by specific capacitance and operating voltage.
Land Registry on Blockchain (2020)

The commercial real estate market is a significant part of the global economy, currently dominated by a small set of firms and organizations that lack transparency. The process of property transfers also requires third party intervention which is expensive. In many countries, the process of title transfers is problematic. We are still in the initial steps of digitization, due to the improvement required in terms of use of technology to represent assets in digital forms. Increase in liquidity of investments and purchases, proper management, documentation as well as ease of access is the future of real estate. Blockchain technologies have the potential to drive these changes as explained in Chapter 1. Blockchain technologies like Ethereum[1] include asset tokenization, and act as immutable and decentralized transaction ledgers. Tokens on the ledger can represent the real estate assets. Particulary, the non-fungible tokens on Ethereum can serve as a representation of transfer of resources. Ethereum grants trusted and distributed smart contracts for token operations. My project is a system for real estate cadastral record keeping and title transfers that uses the ERC-721 specification[2] related to the non-fungible tokens. Testing of the implementation is done using Government records from District of Columbia[3]. Ethereum is a natural choice for this project due to its evolution, an active development community, and many supporting languages and tools that facilitate use of smart contracts. This paper gives the background, implementation details and significance of such a system. Some cost-related and defensive mechanisms offered by the system are discussed later in the report.
Increased rho kinase activity in temporal artery biopsies from patients with giant cell arteritis (2014)

Subjects with GCA had more intense pERM staining in TAB specimens compared with age- and sex-matched controls, regardless of whether TAB was positive or negative by routine histopathology, suggesting increased ROCK activity in GCA. The ROCK pathway warrants further investigation in GCA, as it may have diagnostic significance in enhancing the sensitivity of TAB.
Mycolactone reveals the substrate-driven complexity of Sec61-dependent transmembrane protein biogenesis (2017)

Mycolactone is the exotoxin virulence factor produced by <i>Mycobacterium ulcerans</i>, the pathogen responsible for Buruli ulcer. The skin lesions and immunosuppression that are characteristic of this disease result from the action of mycolactone, which targets the Sec61 complex and inhibits the co-translational translocation of secretory proteins into the endoplasmic reticulum. In this study, we investigate the effect of mycolactone on the Sec61-dependent biogenesis of different classes of transmembrane protein (TMP). Our data suggest that the effect of mycolactone on TMP biogenesis depends on how the nascent chain initially engages the Sec61 complex. For example, the translocation of TMP lumenal domains driven by an N-terminal cleavable signal sequence is efficiently inhibited by mycolactone. In contrast, the effect of mycolactone on protein translocation that is driven solely by a non-cleavable signal anchor/transmembrane domain depends on which flanking region is translocated. For example, while translocation of the region N-terminal to a signal anchor/transmembrane domain is refractive to mycolactone, C-terminal translocation is efficiently inhibited. Our findings highlight the diversity of Sec61-dependent translocation and provide a molecular basis for understanding the effect of mycolactone on the biogenesis of different TMPs.
Low-Power Flip-Flop Using Internal Clock Gating And Adaptive Body Bias (2006)

This dissertation presents a new systematic approach to flip-flop design using Internal Clock Gating, (ICG), and Adaptive Body-Bias, (ABB), in order to reduce power consumption. The process requires careful transistor resizing in order to maintain signal integrity and the functionality of the flip-flop at the target frequency.A novel flip-flop architecture, based on the Transmission Gate Flip-Flop, (TGFF), which incorporated ICG and ABB techniques, was designed. This architecture was simulated intensively in order to determine under what conditions its use is appropriate. In addition, it was necessary to establish a methodology for creating a standard testbench and environment setup for the required Hspice simulations. Software tools were written in C++ and Perl in order to facilitate the interface between Cadence Design Tools and Hspice.The new flip-flop, which was named the Low-Power Flip-Flop, (LPFF), was compared to the Transmission-Gate Flip-Flop, (TGFF), and to the Transmission-Gate with Clock-Gating Flip-Flop, (TGCGFF). Comprehensive Hspice simulations of the three flip-flop designs, implemented with Bsim3v3 transistor models for TSMC 180 nm technology, were used as the means of comparison.Simulations demonstrated that the new flip-flop is appropriate for applications that require low switching activity. In such a situation the LPFF consumes 7.8% to 95.7% less power than the TGFF and 0.8% to 23.7% less power than the TGCGFF. Power savings obtained by the LPFF increase as the length of the period with no switching activity increases, especially when the input data is all zeros. The trade-off is an increase in the D-to-Q delays and in the flip-flop area. The LPFF presented D-to-Q delays of 60% to 69% longer than the delays of the TGFF and 9% to 11% longer than the delays of the TGCGFF. The LPFF cells require an area that is 15% to 34% larger than the TGFF cells and 6% to 17% larger than the TGCGFF cells.
Monitoring the influence of light intensity on the growth and mortality of duckweed (Lemna minor) through digital images processing (2013)

The growth of duckweed (Lemna minor), depending on biotic and abiotic factors (i.e. temperature, light intensity, photoperiod, pH, nutrients), is an important compartment of the treatment process in wastewater floating macrophytes ponds. Excess or shortage of this biomass might be responsible of the dysfunction of such ponds. Modeling these duckweed ponds through mass balances based on Petersen’s matrix should help in an optimal management of such facilities. This article focused on (i) the influence of light intensity on the growth, and (ii) the mortality of Lemna minor under a constant temperature. Experiments were carried out in a growth chamber using a pilot consisting of transparent cubic tanks, with an initial fresh Lemna minor biomass. In order to monitor Lemna minor biomass, digital image processing was achieved in addition to fresh weight and dry weight measurements methods. The results showed that Lemna minor reached a maximum growth rate (0.19 d) for light intensities ranging between 250 μmol.m2.s and 300 μmol.m2. s. Light intensities from 300 μmol.m 2.s to 400 μmol.m2.s induced a slight growth inhibition. This inhibition was strongly marked at 450 μmol.m2.s. As for mortality, very low levels (< 0.05 d) were determined. These results thus provide a contribution in the modeling of duckweed waste stabilization ponds.
Những nhận thức mới về chủ nghĩa xã hội và xây dựng chủ nghĩa xã hội ở Việt Nam (2012)

Sau hơn 20 nam đổi mới dưới sự lanh đạo của Đảng Cộng sản Việt Nam, đất nước ta đa đạt được những thanh tựu to lớn, co ý nghĩa lịch sử đối với vận mệnh của dân tộc. Thanh tựu của cong cuộc đổi mới đa va đang tạo nen thế va lực mới để Đảng va nhân dân ta thực hiện hệ mục tieu: dân giau, nước mạnh, dân chủ, cong bằng, van minh. Lam ro them những nhận thức mới của Đảng về chủ nghĩa xa hội va xây dựng chủ nghĩa xa hội trong hơn 25 nam đổi mới va thực hiện Cương lĩnh xây dựng đất nước trong thời kỳ qua độ len chủ nghĩa xa hội (1991), từ đo neu len một số định hướng cơ bản va giải phap chủ yếu tiếp tục bổ sung, phat triển lý luận về chủ nghĩa xa hội, xây dựng chủ nghĩa xa hội ở Việt Nam la những nội dung chinh của cuốn sach. Hải Thanh giới thiệu
Signal Transducer and Activator of Transcription 6 Is Essential in the Induction of Contact Hypersensitivity (2000)

Contact hypersensitivity (CHS) is thought to be mainly associated with the activation of T helper type 1 (Th1) cells. However, there is also evidence that Th2 cells or Th2 cytokines play a role in the development of CHS. To analyze the functional contribution of Th2 cytokines interleukin (IL)-4 and IL-13, signal transducer and activator of transcription 6 (STAT6)-deficient (STAT6(-/)-) and wild-type (wt) control C57BL/6 mice were contact sensitized with 5% 2,4,6-trinitrochlorobenzene (TNCB), 0.5% 2,4-dinitrofluorobenzene, or 5% 4-ethoxyl methylene-2-phenyl-2-oxazolin-5-one, and any skin reactions were examined. Ear swelling was significantly reduced with a delayed peak response in STAT6(-/)- mice compared with wt mice.A histological analysis revealed that the infiltration of both eosinophils and neutrophils in the skin challenged after 24 h in STAT6(-/)- mice decreased substantially compared with that in wt mice. The expression of Th2 cytokines (IL-4, IL-5) in TNCB-challenged skin tissues and the supernatants from T cells stimulated by 2,4,6-trinitrobenzene sulfonate-modified spleen cells, as well as the immunoglobulin (Ig)E and IgG1 response after challenge, were also profoundly reduced in STAT6(-/)- mice, whereas the expression of interferon gamma was the same in STAT6(-/)- and wt mice after challenge. Furthermore, adoptive transfer experiments revealed that STAT6(-/)- mice induced CHS after injection of lymph node cells obtained from sensitized wt mice. Our data suggest that the STAT6 signal plays a critical role in the induction phase of CHS.
New Series of Antiprion Compounds:  Pyrazolone Derivatives Have the Potent Activity of Inhibiting Protease-Resistant Prion Protein Accumulation (2007)

To find effective antiprion compounds, we synthesized and evaluated various pyrazolone derivatives. Seven of 19 compounds showed inhibition of PrP-res accumulation and the remarkably active compound 13 showed an IC50 value of 3 nM in both ScN2a and F3 cell lines. Findings from studies on physicochemical and biochemical properties suggest that the action mechanism of these compounds does not correlate with any antioxidant activities, any of hydroxyl radical scavenging activities, or any SOD-like activities.
Creating single-atom Pt-ceria catalysts by surface step decoration (2016)

Single-atom catalysts maximize the utilization of supported precious metals by exposing every single metal atom to reactants. To avoid sintering and deactivation at realistic reaction conditions, single metal atoms are stabilized by specific adsorption sites on catalyst substrates. Here we show by combining photoelectron spectroscopy, scanning tunnelling microscopy and density functional theory calculations that Pt single atoms on ceria are stabilized by the most ubiquitous defects on solid surfaces--monoatomic step edges. Pt segregation at steps leads to stable dispersions of single Pt(2+) ions in planar PtO4 moieties incorporating excess O atoms and contributing to oxygen storage capacity of ceria. We experimentally control the step density on our samples, to maximize the coverage of monodispersed Pt(2+) and demonstrate that step engineering and step decoration represent effective strategies for understanding and design of new single-atom catalysts.
SKU assignment to unidirectional picking lines using correlations (2015)

A real life order picking system consisting of a set of unidirectional picking lines is investigated. Batches of stock keeping units (SKUs) are processed in waves defined as a set of SKUs and their corresponding store requirements. Each wave is processed independently on one of the parallel picking lines as pickers walk in a clockwise direction picking stock. Once all the orders for a wave are completed a new mutually exclusive set of SKUs are brought to the picking line for a new wave. SKUs which differ only in size classification, for example small, medium and large shirts, are grouped together into distributions (DBNs) and must be picked in the same wave. The assignment of DBNs to available picking lines for a single day of picking is considered in this paper.Â Different assignments of DBNs to picking lines are evaluated using three measures, namely total walking distance, the number of resulting small cartons and work balance. Several approaches to assign DBNs to picking lines have been investigated in literature. All of these approaches seek to minimise walking distance only and include mathematical formulations and greedy heuristics. Four different correlation measure are introduced in this paper to reduce the number of small cartons produced and reduce walking distance simultaneously. These correlation measures are used in a greedy insertion algorithm.Â The correlation measures were compared to historical assignments as well as a greedy approach which is known toÂ address walking distances effectively. Using correlation measures to assign DBNs to picking lines reduces the total walking distance of pickers by 20% compared to the historical assignments. This is similar to the greedy approach which only considers walking distance as an objective, however, using correlations reduced the number of small cartons produced by the greedy approach.
Bioanalytical Methods for Food Allergy Diagnosis, Allergen Detection and New Allergen Discovery (2015)

For effective monitoring and prevention of the food allergy, one of the emerging health problems nowadays, existing diagnostic procedures and allergen detection techniques are constantly improved. Meanwhile, new methods are also developed, and more and more putative allergens are discovered. This review describes traditional methods and summarizes recent advances in the fast evolving field of the in vitro food allergy diagnosis, allergen detection in food products and discovery of the new allergenic molecules. A special attention is paid to the new diagnostic methods under laboratory development like various immuno- and aptamer-based assays, including immunoaffinity capillary electrophoresis. The latter technique shows the importance of MS application not only for the allergen detection but also for the allergy diagnosis.
A novel approach to feedforward higher-harmonic control (1994)

A novel adaptive filter structure is proposed for the control of systems characterized by higher harmonic response. The control approach has been designated the higher harmonic least-mean squares (HLMS) algorithm to differentiate it from the standard multifrequency filtered-x version of the least-mean squares approach. In the HLMS algorithm, a single frequency reference at that of the fundamental is all that is required to implement the controller. The remaining harmonics are generated internally based upon simple trigonometric relationships. The filtered-x LMS algorithm is implemented in parallel for each frequency to be controlled, minimizing or totally eliminating the contribution of the time-varying terms during the convergence process and increasing the rate of convergence for the higher harmonic control application. Results from the simulation demonstrate that the HLMS approach is far superior to the standard multifrequency, filtered-x LMS algorithm in adaptive, feedforward, higher-harmonic control.
Synthesis of cytosine radiolysis products: cis- and trans-1-carbamoyl-4,5-dihydroxyimidazolidin-2-one (1976)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of cytosine radiolysis products: cis- and trans-1-carbamoyl-4,5-dihydroxyimidazolidin-2-oneNelson J. Leonard and David F. WiemerCite this: J. Am. Chem. Soc. 1976, 98, 25, 8218–8221Publication Date (Print):December 1, 1976Publication History Published online1 May 2002Published inissue 1 December 1976https://pubs.acs.org/doi/10.1021/ja00441a055https://doi.org/10.1021/ja00441a055research-articleACS PublicationsRequest reuse permissionsArticle Views155Altmetric-Citations7LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Comparison of Commercially Available and Laboratory-Developed Assays for <i>In Vitro</i> Detection of SARS-CoV-2 in Clinical Laboratories (2020)

Multiple laboratory-developed tests (LDTs) and commercially available assays have emerged to meet diagnostic needs related to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. To date, there is limited comparison data for these different testing platforms. We compared the analytical performance of a LDT developed in our clinical laboratory based on CDC primer sets and four commercially available, FDA emergency use authorized assays for SARS-CoV-2 (Cepheid, DiaSorin, Hologic Panther, and Roche Cobas) on a total of 169 nasopharyngeal swabs. The LDT and Cepheid Xpert Xpress SARS-CoV-2 assays were the most sensitive assays for SARS-CoV-2 with 100% agreement across specimens. The Hologic Panther Fusion, DiaSorin Simplexa, and Roche Cobas 6800 failed to detect positive specimens only near the limit of detection of our CDC-based LDT assay. All assays were 100% specific, using our CDC-based LDT as the gold standard. Our results provide initial test performance characteristics for SARS-CoV-2 reverse transcription-PCR (RT-PCR) and highlight the importance of having multiple viral detection testing platforms available in a public health emergency.
Ovarian hyperstimulation syndrome in the 21st century (2015)

GnRHa trigger is currently the best tool we have to prevent OHSS and at the same time maintain good reproductive outcomes. Future research will explore the safety and efficacy of kisspeptin trigger.