gebhart/patrepeval huggingface dataset:
A First for Human Vaccinology: GMP Compliant Radiation Attenuation of Plasmodium falciparum Sporozoites for Production of a Vaccine Against Malaria (2022)

Ionizing radiation (UV, X-ray and ɣ) administered at an appropriate dose to pathogenic organisms can prevent replication while preserving metabolic activity. We have established the GMP process for attenuation by ionizing radiation of the <i>Plasmodium falciparum</i> (Pf) sporozoites (SPZ) in Sanaria<sup>®</sup> PfSPZ Vaccine, a protective vaccine against malaria. Mosquitoes raised and infected aseptically with Pf were transferred into infected mosquito transport containers (IMTC) and ɣ-irradiated using a <sup>60</sup>Co source. PfSPZ were then extracted, purified, vialed, and cryopreserved. To establish the appropriate radiation conditions, the irradiation field inside the IMTCs was mapped using radiochromic film and alanine transfer dosimeters. Dosimeters were irradiated for times calculated to provide 120-170 Gy at the minimum dose location inside the IMTC and regression analysis was used to determine the time required to achieve a lower 95% confidence interval for 150 Gy. A formula incorporating the half-life of <sup>60</sup>Co was then used to construct tables of irradiation times for each calendar day. From the mapping studies, formulae were derived to estimate the minimum and maximum doses of irradiation received inside the IMTC from a reference dosimeter mounted on the outside wall. For PfSPZ Vaccine manufacture a dose of 150 Gy was targeted for each irradiation event, a dose known to completely attenuate PfSPZ. The reference dosimeters were processed by the National Institute of Standards and Technology. There have been 587 irradiation events to produce PfSPZ Vaccine during 13 years which generated multiple lots released for pre-clinical studies and clinical trials. The estimated doses at the minimum dose location (mean 154.3 ± 1.77 Gy; range 150.0-159.3 Gy), and maximum dose location (mean 166.3 ± 3.65 Gy, range 155.7 to 175.3 Gy), in IMTCs were normally distributed. Overall dose uniformity was 1.078 ± 0.012. There was no siginifcant change in measured dose over 13 years. As of January 2022, 21 clinical trials of PfSPZ Vaccine have been conducted, with 1,740 volunteers aged 5 months to 61 years receiving 5,648 doses of PfSPZ Vaccine totalling >5.3 billion PfSPZ administered. There have been no breakthrough infections, confirming the consistency and robustness of the radiation attenuation process.
Stoffwechselprodukte von Mikroorganismen, 182. Aufklärung der Struktur des Nucleosidantibiotikums Nikkomycin X (1979)

Abstract Neben dem bereits früher beschriebenen Nikkomycin Z, dessen Struktur bestätigt werden konnte, wurde aus dem Kulturfiltrat von Streptomyces tendae eine weitere die Chitinsynthase hemmende Komponente, Nikkomycin X, isoliert, die mit Nikkomycin Z strukturisomer ist, aber anstelle von Uracil 4‐Formyl‐4‐imidazolin‐2‐on enthält. Die Struktur wurde mit spektroskopischen Methoden, durch chemischen Abbau und durch Teilsynthesen bewiesen. Die biologischen Aktivitäten und die Wirkungsspektren der beiden Nikkomycine sind ähnlich.
A new modal power distribution measurement for high-speed laser transmitters (2002)

We propose and demonstrate a new technique for measuring the modal power distribution of light launched into a multimode fiber. When applied to high-speed lasers our method is much simpler than existing ones.
Enhanced Immunity to Breast Cancer in Mice Immunized With Fibroblasts Transfected With a Complementary DNA Expression Library From Breast Cancer Cells (2006)

Breast cancer cells express an array of weakly immunogenic tumor-associated antigens (TAAs). Under appropriate circumstances, immunity to breast cancer can be induced, with potential benefits for patients with the disease. Here, we report a new cell-based vaccination strategy resulting in enhanced immunity to breast cancer in tumor-bearing mice. The strategy was designed to enrich the vaccine for highly immunogenic cells. The vaccine was prepared by transfer of unfractionated complementary DNA (cDNA) derived from a highly malignant breast neoplasm that arose spontaneously in a C3H/He mouse (SB5b) into an immunogenic fibroblast cell line. As the transferred cDNA spontaneously integrates into the genome of the recipient cells and is replicated as the cells divide, sufficient DNA to prepare the vaccine could be obtained from as few as 10(7) cells (4-mm tumor). Because only a small proportion of the transfected cell population was expected to have incorporated genes responsible for inducing immunity to the breast cancer, we devised a novel approach designed to enrich the transfected cell population for cells that induced immunity to the neoplasm. Aliquots of the transfected population were divided into small pools (initial inoculum = 4 x 10). Afterward, the cell number from each pool was allowed to expand in vitro. Pools containing greater numbers of immunogenic cells (identified by 2 independent assays) were subdivided for additional rounds of immune selection. Enhanced immunity to the neoplasm was detected in tumor-bearing mice treated solely by immunization with the enriched cell population. The immunity, mediated by CD8+ T cells, was sufficient to prolong the survival of mice with established breast cancer.
Analysis of Pulsating Flow Measurement of Engine Exhaust by Pitot Tube Flow Meter (2004)

Pitot tube flow metering technique has been used to measure pulsating flow from a vehicles engine exhaust. In general, flow metering techniques that utilize differential pressure measurements based on the Bernoulli's theory likely to show erroneous reading when measuring an average flow rate of pulsating flow. The primary reason for this is the non-linear relationship between the differential pressure and the flow rate, i.e. the flow rate is proportional to the square root of the differential pressure. Thus, an average of the differential pressure does not give an average of pulsating flow. In the present study, fast response pressure transducers have been used to measure the pulsating pressure as raw as possible. Then, the pulsating differential pressure is converted to flow rate keeping the pulsation not averaged. An average of the flow rate is then calculated in flow rate domain in order to maintain the linearity of before and after the averaging. The peak amplitude of a pulsation measured here was about 1800 LPM at the average flow rate of 70 LPM when an engine was idling. This measurement has been confirmed by measuring the pulsation with a gas analyzer. The result here implies large amount of back and forth gas movement in the exhaust tube. Such amount of pulsation can cause maximum five times higher erroneous result with the pressure domain averaging when compared to a flow rate domain averaging.
Optical polarization characteristics of<i>m</i>-oriented InGaN/GaN light-emitting diodes with various indium compositions in single-quantum-well structure (2008)

Spontaneously polarized light emission from m-plane InGaN/GaN light-emitting diodes was studied as a function of In composition in the InGaN single quantum-well layer. Emission wavelength was varied between 394 and 472 nm. A strong correlation was confirmed between optical polarization and In composition; the higher the In composition, the stronger the optical polarization. The photon-energy difference between the emission spectra associated with the two polarizations, ΔM, was evaluated as a function of current. ΔM exhibited a negative monotonic current dependence for the 394 nm emitting sample and the dependence was changed to positive monotonic as the wavelength became longer towards 472 nm. This change was tentatively attributed to the valence band mixing and the crystal momentum conservation that became relevant with the band filling. ΔM and optical polarization exhibited only a moderate correlation; the Fermi–Dirac function has been used to explain the weakened optical polarization under increased current injection.
An electron gun for a sheet beam klystron (2003)

Calabazas Creek Research, Inc.(CCR) is developing a rectangular, gridded, thermionic, dispenser-cathode gun for sheet beam devices. We present the preliminary design of a 415 kV, 250 A gridded gun for a sheet beam klystron, giving results from 2D and 3D simulations.
Effect of humidity treatments on porosity and mechanical integrity of mesoporous silica films (2003)

The beneficial effects humidity treatments have on molecularly templated mesoporous silica films with respect to elastic modulus, with minimal detrimental effects on porosity and dielectric constant, were identified.
Mutational Analysis of the Repeated Open Reading Frames, ORFs 63 and 70 and ORFs 64 and 69, of Varicella-Zoster Virus (2001)

Varicella-zoster virus (VZV) open reading frame 63 (ORF63), located between nucleotides 110581 and 111417 in the internal repeat region, encodes a nuclear phosphoprotein which is homologous to herpes simplex virus type 1 (HSV-1) ICP22 and is duplicated in the terminal repeat region as ORF70 (nucleotides 118480 to 119316). We evaluated the role of ORFs 63 and 70 in VZV replication, using recombinant VZV cosmids and PCR-based mutagenesis to make single and dual deletions of these ORFs. VZV was recovered within 8 to 10 days when cosmids with single deletions were transfected into melanoma cells along with the three intact VZV cosmids. In contrast, VZV was not detected in transfections carried out with a dual deletion cosmid. Infectious virus was recovered when ORF63 was cloned into a nonnative AvrII site in this cosmid, confirming that failure to generate virus was due to the dual ORF63/70 deletion and that replication required at least one gene copy. This requirement may be related to our observation that ORF63 interacts directly with ORF62, the major immediate-early transactivating protein of VZV. ORF64 is located within the inverted repeat region between nucleotides 111565 and 112107; it has some homology to the HSV-1 Us10 gene and is duplicated as ORF69 (nucleotides 117790 to 118332). ORF64 and ORF69 were deleted individually or simultaneously using the VZV cosmid system. Single deletions of ORF64 or ORF69 yielded viral plaques with the same kinetics and morphology as viruses generated with the parental cosmids. The dual deletion of ORF64 and ORF69 was associated with an abnormal plaque phenotype characterized by very large, multinucleated syncytia. Finally, all of the deletion mutants that yielded recombinants retained infectivity for human T cells in vitro and replicated efficiently in human skin in the SCIDhu mouse model of VZV pathogenesis.
Physics and Technology of Transparent Ceramic Armor: Sintered Al2O3 vs Cubic Materials (2006)

Sintered sub-micrometer alumina (alpha-Al2O3) is the hardest transparent armor. However, its trigonal structure gives rise to a strong thickness effect that makes thicker components translucent. Cubic ceramics (no birefringence!), on the other hand, are less hard but may exhibit an improved transmission. Known cubic armor ceramics are manufactured in a way resulting in coarse microstructures with low hardness. Therefore, a cubic sub-micrometer spinel was developed here that associates a high hardness > 14 GPa with an in-line transmission approaching the theoretical limit - with the consequence that the thickness effect becomes very small, and transparent components can be manufactured with several mm thickness without loss of transmissivity. Additionally, weight benefits at high protective strength are expected because the hard components can be designed thinner than using known spinel or AlON grades.
Analytical properties of time-of-flight PET data (2008)

We investigate the analytical properties of time-of-flight (TOF) positron emission tomography (PET) sinograms, where the data are modeled as line integrals weighted by a spatially invariant TOF kernel. First, we investigate the Fourier transform properties of 2D TOF data and extend the 'bow-tie' property of the 2D Radon transform to the time-of-flight case. Second, we describe a new exact Fourier rebinning method, TOF-FOREX, based on the Fourier transform in the time-of-flight variable. We then combine TOF-FOREX rebinning with a direct extension of the projection slice theorem to TOF data, to perform fast 3D TOF PET image reconstruction. Finally, we illustrate these properties using simulated data.
Estimation of Galileo Uncalibrated Hardware Delays for Ambiguity-Fixed Precise Point Positioning (2016)

Ambiguity fixing in Precise Point Positioning (PPP) has been extensively studied in recent years. The provision of uncalibrated hardware delays (UHDs) to the PPP algorithm, on top of precise orbits and clocks, allows the recovery of the integer values of the carrier-phase ambiguities. Experimental results show that integer ambiguity resolution increases the accuracy in the position domain. Most of the research so far has been done on GPS, where the wide- and narrow-lane approach for ambiguity resolution has proven successful. In the context of multi-GNSS PPP, the aim of this study is to extend the method to Galileo. Initial results for UHD estimation for Galileo satellites are presented. The contribution of ambiguity-fixing to GPS + Galileo PPP is assessed. Copyright © 2016 Institute of Navigation Copyright © 2016 Institute of Navigation
Visualizing Thermally Activated Memristive Switching in Percolating Networks of Solution‐Processed 2D Semiconductors (2021)

Abstract Memristive systems present a low‐power alternative to silicon‐based electronics for neuromorphic and in‐memory computation. 2D materials have been increasingly explored for memristive applications due to their novel biomimetic functions, ultrathin geometry for ultimate scaling limits, and potential for fabricating large‐area, flexible, and printed neuromorphic devices. While the switching mechanism in memristors based on single 2D nanosheets is similar to conventional oxide memristors, the switching mechanism in nanosheet composite films is complicated by the interplay of multiple physical processes and the inaccessibility of the active area in a two‐terminal vertical geometry. Here, the authors report thermally activated memristors fabricated from percolating networks of diverse solution‐processed 2D semiconductors including MoS 2 , ReS 2 , WS 2 , and InSe. The mechanisms underlying threshold switching and negative differential resistance are elucidated by designing large‐area lateral memristors that allow the direct observation of filament and dendrite formation using in situ spatially resolved optical, chemical, and thermal analyses. The high switching ratios (up to 10 3 ) that are achieved at low fields (≈4 kV cm −1 ) are explained by thermally assisted electrical discharge that preferentially occurs at the sharp edges of 2D nanosheets. Overall, this work establishes percolating networks of solution‐processed 2D semiconductors as a platform for neuromorphic architectures.
Investigation of performance of fire-tube boilers integrated with ion transport membrane for oxy-fuel combustion (2016)

The performance of a carbon free fire-tube boiler utilizing two-pass oxygen transport reactors was numerically investigated. The influences of the oxygen transport reactors wall temperature on the reaction rate, oxygen permeation and heat flux were quantified. The performance of the reactors has been investigated at elevated temperature. It is observed that both heat transfer and combustion characteristics can be optimized at an elevated temperature of 1373 K. Increasing the mass fraction of methane in this reactor to 6% results in improvement of the heat transfer and combustion characteristics in the reactor. Further increase in CH4% did not lead to any significant improvement. The fuel flow rate variation did not have any significant impact on the reactor performance. It is indicated that the membrane temperature has significant effect on the reaction rates and oxygen flux in the upstream region in particular. Copyright © 2016 John Wiley & Sons, Ltd.
Fiber-Optic Probes for in Vivo Raman Spectroscopy in the High-Wavenumber Region (2005)

In vivo Raman spectroscopy, using fiber-optic probes is hindered by the intense background signal, which is generated in the fused-silica fibers, in the fingerprint region of the Raman spectrum (approximately 0-2000 cm(-1)). Optical filtering is necessary to obtain tissue spectra of sufficient quality. The complexity of fiber-optic probes for fingerprint Raman spectroscopy, in combination with size constraints and flexibility requirements for in vivo use have been a major obstacle in the development of in vivo diagnostic tools based on Raman spectroscopy. A setup for remote Raman spectroscopic tissue characterization in the high-wavenumber region ( approximately 2400-3800 cm(-1)) is presented. It makes use of a single, unfiltered, optical fiber for guiding laser light to the sample and for collecting scattered light and guiding it back to a spectrometer. Such a simple configuration is possible because the fused-silica core and cladding of the fiber present almost no Raman background signal at these wavenumbers. Several commercially available optical fibers were tested with respect to Raman signal background, to determine their suitability for in vivo Raman spectroscopy measurements in the high-wavenumber region. Different fiber core, cladding, and coating materials were tested. Silica core-silica clad fibers, with an acrylate coating and a black nylon jacket, proved to be one of the best candidates. In vitro measurements on brain tissue of a 6-month-old pig were obtained with a remote high-wavenumber Raman setup. They illustrate the low background signal generated in the setup and the signal quality obtained with a collection time of 1 s.
Design trade-offs in signal component separators for outphasing power amplifiers (2013)

Implementation design space of piece-wise linear outphasing signal component separator is explored by utilizing the changes in micro-architecture, choice of storage elements and aggressive back-end leakage power optimization techniques. With combination of these techniques, ~2× energy and area savings are achieved, resulting in record energy-efficiency of 32pJ/sample for asymmetric multilevel outphasing and 22pJ/sample for linear amplification with nonlinear components, at throughput of 400MSample/s and areas of 0.2-0.4mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> in 45nm SOI process.
Supported ionic liquid catalysis investigated for hydrogenation reactions (2002)

The concept of supported ionic liquid catalysis (silc) has successfully been applied to hydrogenation reactions, which significantly reduced the required amounts of ionic liquid phase and enabled the usage of fixed-bed technology; the resulting catalysts exhibited high activity and outstanding stability.
Quantitative computerized analysis of diffuse lung disease in high‐resolution computed tomography (2003)

An automated computerized scheme has been developed for the detection and characterization of diffuse lung diseases on high-resolution computed tomography (HRCT) images. Our database consisted of 315 HRCT images selected from 105 patients, which included normal and abnormal slices related to six different patterns, i.e., ground-glass opacities, reticular and linear opacities, nodular opacities, honeycombing, emphysematous change, and consolidation. The areas that included specific diffuse patterns in 315 HRCT images were marked by three radiologists independently on the CRT monitor in the same manner as they commonly describe in their radiologic reports. The areas with a specific pattern, which three radiologists marked independently and consistently as the same patterns, were used as "gold standard" for specific abnormal opacities in this study. The lungs were first segmented from the background in each slice by use of a morphological filter and a thresholding technique, and then divided into many contiguous regions of interest (ROIs) with a 32x32 matrix. Six physical measures which were determined in each ROI included the mean and the standard deviation of the CT value, air density components, nodular components, line components, and multilocular components. Artificial neural networks (ANNs) were employed for distinguishing between seven different patterns which included normals and six patterns associated with diffuse lung disease. The sensitivity of this computerized method for a detection of the six abnormal patterns in each ROI was 99.2% (122/123) for ground-glass opacities, 100% (15/15) for reticular and linear opacities, 88.0% (132/150) for nodular opacities, 100% (98/98) for honeycombing, 95.8% (369/385) for emphysematous change, and 100% (43/43) for consolidation. The specificity in detecting a normal ROI was 88.1% (940/1067). This computerized method may be useful in assisting radiologists in their assessment of diffuse lung disease in HRCT images.
Identification of a Novel Phosphorylation Site in Protein Phosphatase Inhibitor-1 as a Negative Regulator of Cardiac Function (2006)

Human and experimental heart failure is characterized by increases in type-1 protein phosphatase activity, which may be partially attributed to inactivation of its endogenous regulator, protein phosphatase inhibitor-1. Inhibitor-1 represents a nodal integrator of two major second messenger pathways, adenosine 3',5'-cyclic monophosphate (cAMP) and calcium, which mediate its phosphorylation at threonine 35 and serine 67, respectively. Here, using recombinant inhibitor-1 wild-type and mutated proteins, we identified a novel phosphorylation site in inhibitor-1, threonine 75. This phosphoamino acid was phosphorylated in vitro by protein kinase Calpha independently and to the same extent as serine 67, the previous protein kinase Calpha-identified site. Generation of specific antibodies for the phosphorylated and dephosphorylated threonine 75 revealed that this site is phosphorylated in rat and dog hearts. Adenoviral-mediated expression of the constitutively phosphorylated threonine 75 inhibitor-1 in isolated myocytes was associated with specific stimulation of type-1 protein phosphatase activity and marked inhibition of the sarcoplasmic calcium pump affinity for calcium, resulting in depressed contractility. Thus, phosphorylation of inhibitor-1 at threonine 75 represents a new mechanism of cardiac contractility regulation, partially through the alteration of sarcoplasmic reticulum calcium transport activity.
Clinical Courses of Seven Survivors of Clostridium septicum Infection and Their Immunologic Responses to   Toxin (1994)

Clostridium septicum bacteremia typically portends a fulminant disease associated with high mortality. We describe the clinical courses of seven survivors of C. septicum infection and their antibody responses to the alpha toxin produced by C. septicum. Three patients had clinical syndromes ranging from uncomplicated bacteremia to early typhlitis, and three patients had syndromes ranging from abscess to myonecrosis and septic shock. In addition, an AIDS patient who developed septic shock and who had extensive gas in the retroperitoneal musculature did not undergo surgery but survived after receiving antimicrobial therapy and intensive supportive care. Both immunocompetent patients with myonecrosis had detectable IgG to alpha toxin by immunoblot analysis. IgG to alpha toxin was not detected in the four immunocompetent patients who had C. septicum bacteremia but who did not have myonecrosis or in the AIDS patient with myonecrosis. Therefore, humoral responses to alpha toxin during C. septicum infection may be related to the host's clinical syndrome and immune status.
Calixpyrroles (1998)

The calix[4]pyrroles are a class of old but new heterocalixarene analogues that show interesting anion and neutral substrate binding properties. Calix[4]pyrroles are easy to make and functionalize. As such, they have been employed in the production of separation media for anionic and neutral species. Calixpyrroles also provide useful precursors for the generation of novel calixpyridinopyrroles and calixpyridines.