gebhart/patrepeval huggingface dataset:
Isolation and characterization of the two major apoproteins in human lipoprotein [a]. (1986)

Human Lp[a] was isolated in preparative amounts from two donors; the native lipoprotein and its constituent apoproteins, apo[a] and apoB, were characterized extensively. Based on differences in apparent molecular weight, four different isoforms of apo[a], a1-a4, were observed between the two donors. The number and relative distribution of these isoforms varied between donors but were constant for each donor. Each apo[a] isoform was shown to be derived from a discrete apo[a]-B100 disulfide-linked complex present before reduction. Complete delipidation of Lp[a] was followed by solubilization, reduction, and carboxamidomethylation of the constituent apoproteins. These apoproteins were then separated by immunoaffinity chromatography using anti-apo[a]- or anti-apoB-Sepharose; their purity and structural integrity were demonstrated by Western blot analysis. ApoB isolated by this procedure was essentially identical to apoB from autologous LDL with respect to molecular weight, secondary structure, amino acid composition, and sialic acid content. However, apo[a] differed from apoB in that it exhibited: a much less alpha-helical, less beta, but much more disordered structure; a lower proportion of aspartate, isoleucine, leucine, phenylalanine, and lysine, but a higher proportion of proline, glycine, and threonine; and a much higher content of sialic acid. These results indicate that apo[a] is not a superglycosylated form of apoB but is distinctly different in its composition and structure.
Human DNA topoisomerase II: biochemistry and role in chemotherapy resistance (review). (1996)

Recently, it has been discovered that DNA topoisomerases are the target of many anti-cancer drugs which were already widely used in the clinic. Using the latest molecular biological techniques much has been learned about the function of DNA topoisomerases in normal cells and in cancer cells, and about how anticancer drugs inhibit these enzymes. In this review we present an overview of the function of human type II DNA topoisomerases, how they are inhibited by certain drugs and on how cancer cells may become resistant to these drugs.
The 1974 origins of VoIP (2005)

This paper brings light on the digital signal processing (DSP) roots of a modern concept, voice over IP (VoIP). An example is also provided in which developments in DSP - speech coding, in particular - had a profound impact on the early development of the ARPANET, the ancestor of the Internet. The author shows how packet speech, recently rediscovered and made popular as VoIP, was first successfully demonstrated in 1974 on the ARPANET and how the Internet protocol (IP) emerged largely as a result of that effort.
The effects of male age on sperm DNA damage in healthy non-smokers (2006)

Our findings indicate that (i) older men have increased sperm DNA damage associated with alkali-labile sites or single-strand DNA breaks and (ii) independent of age, men with substantial daily caffeine consumption have increased sperm DNA damage associated with double-strand DNA breaks. DNA damage in sperm can be converted to chromosomal aberrations and gene mutations after fertilization, increasing the risks of developmental defects and genetic diseases among offspring.
Improving Design using Autonomous Spatial and Structural Generators (2005)

During a building design process, a structural designer transforms a spatial design into a structural design and this structural system can be improved by optimisation methods or expert views of other structural designers. The improved structural system allows the architect a new spatial design, which can be transformed or altered again by the architect. This design cycle can be repeated several times and is defined as interaction of spatial and structural design. Case studies are used to demonstrate that this interaction occurs in practice and is needed to improve building quality. This paper presents a program with more or less autonomous spatial and structural generators. Each generator will facilitate one direction in the interaction process. Then using both consecutively leads to a design method that provides interaction between spatial and structural design. For the spatial generator, named Âroom positioning with structural constraintsÂ a space allocation technique is used including constraints that handle structural boundary conditions. A zone generator based on pattern recognition and shape grammars handle the structural design. A Prolog-2 program was developed to demonstrate the application of the two proposed generators. ÂZone generationÂ is performed per building storey and thus represents a horizontal two-dimensional procedure. Similarly Âroom positioning with structural constraintsÂ is a planar vertical operation. In future these procedures can be made three-dimensional.
Tyrphostin AG 556 improves survival and reduces multiorgan failure in canine Escherichia coli peritonitis. (1997)

Tyrosine kinase-dependent cell signaling is postulated to be a pivotal control point in inflammatory responses initiated by bacterial products and TNF. Using a canine model of gram-negative septic shock, we investigated the effect of tyrosine kinase inhibitors (tyrphostins) on survival. Animals were infected intraperitoneally with Escherichia coli 0111: B4, and then, in a randomized, blinded fashion, were treated immediately with one of two tyrphostins, AG 556 (n = 40) or AG 126 (n = 10), or with control (n = 50), and followed for 28 d or until death. All animals received supplemental oxygen, fluids, and antibiotics. Tyrphostin AG 556 improved survival times when compared to controls (P = 0.05). During the first 48 h after infection, AG 556 also improved mean arterial pressure, left ventricular ejection fraction, cardiac output, oxygen delivery, and alveolar-arterial oxygen gradient compared to controls (all P < or = 0.05). These improvements in organ injury were significantly predictive of survival. Treatment with AG 556 had no effect on clearance of endotoxin or bacteria from the blood (both P = NS); however, AG 556 did significantly lower serum TNF levels (P = 0.03). These data are consistent with the conclusion that AG 556 prevented cytokine-induced multiorgan failure and death during septic shock by inhibiting cell-signaling pathways without impairing host defenses as determined by clearance of bacteria and endotoxin.
Predicting odor perceptual similarity from odor structure (2014)

not submitted for publication. Published: 16 April 2014 doi:10.1186/2044-7248-3-S1-O2 Cite this article as: Snitz et al.: Predicting odor perceptual similarity from odor structure. Flavour 2014 3(Suppl 1):O2. Submit your next manuscript to BioMed Central and take full advantage of: • Convenient online submission • Thorough peer review • No space constraints or color figure charges • Immediate publication on acceptance • Inclusion in PubMed, CAS, Scopus and Google Scholar • Research which is freely available for redistribution Submit your manuscript at www.biomedcentral.com/submit Department of Neurobiology, Weizmann Institute of Science, Rehovot 76100, Israel Snitz et al. Flavour 2014, 3(Suppl 1):O2 http://www.flavourjournal.com/content/3/S1/O2 © 2014 Snitz et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Physiological and Molecular Assessment of Altered Expression of <i>Hsc70-1</i> in Arabidopsis. Evidence for Pleiotropic Consequences (2003)

Hsp70s function as molecular chaperones. The protective chaperone activities of hsp70 help to confer tolerance to heat, glucose deprivation, and drought. Overexpression of hsp70s in many organisms correlates with enhanced thermotolerance, altered growth, and development. To better understand the roles of hsp70 proteins in Arabidopsis, the molecular and physiological consequences of altered expression of the major heat shock cognate, Hsc70-1, were analyzed. Extensive efforts to achieve underexpression of Hsc70-1 mRNA using a full-length antisense cDNA resulted in no viable transgenic plants, suggesting that reduced expression is lethal. Constitutive overexpression of Hsc70-1 also appeared to be deleterious to viability, growth, and development because fewer transformants were recovered, and most were dwarfed with altered root systems. Despite being dwarfed, the overexpression plants progressed normally through four selected developmental stages. Heat treatment revealed that Hsc70-1 overexpression plants were more tolerant to heat shock (44 degrees C for 10 min). The elevated basal levels of HSC70-1 in transgenic plants led to delayed heat shock response of several heat shock genes. The data in this study suggest that tight regulation of Hsc70-1 expression is critical for the viability of Arabidopsis and that the functions of HSC70-1 contribute to optimum growth, development, thermotolerance, and regulation of the heat shock response.
Optical Detection of Dangerous Road Conditions (2019)

We demonstrated an optical method to evaluate the state of asphalt due to the presence of atmospheric agents using the measurement of the polarization/depolarization state of near infrared radiation. Different sensing geometries were studied to determine the most efficient ones in terms of performance, reliability and compactness. Our results showed that we could distinguish between a safe surface and three different dangerous surfaces, demonstrating the reliability and selectivity of the proposed approach and its suitability for implementing a sensor.
Polymer aids for settling and filtration of oil sands tailings (2010)

Abstract Commercial Magnafloc 1011 (Percol 727) polymer and in‐house synthesised Al‐PAM polymer were used to flocculate oil sands tailings that were derived from low and high fines oil sands ores. Fines are defined as mineral solids less than 44 µm. The performance of polymers was evaluated in terms of tailings settling, filtration rate, and final moisture content of tilter cakes. Both polymers were shown to effectively flocculate the derived oil sands tailings and hence to enhance tailings settling. Al‐PAM performed very well as a filtration aid. The final moisture content of the filter cake obtained from tailings derived from the low fines ore was 6.6 ± 1.2 wt.% and that from the high fines ore was 16.9 ± 0.8 wt.%. This class of polymer can provide an alternative approach for oil sands tailings disposal that has the potential to eliminate tailings ponds. However, the commercial Magnafloc 1011 polymer was found ineffective as a filtration aid for the two tailings tested in this study.
Hyaluronic acid, an important factor in the wound healing properties of amniotic fluid:<i>In vitro</i>studies of re-epithelialisation in human skin wounds (2013)

Foetal wounds are unique in their ability to heal rapidly without forming scars. The amniotic fluid, rich in nutrients, growth factors, and hyaluronic acid, surrounds the foetus and is essential to foetal wound healing. The wound healing properties of foetal wounds may be the result of high concentrations of hyaluronic acid. This study aimed to verify that amniotic fluid induces re-epithelialisation in human skin wounds in vitro and to study whether this ability is dependent on hyaluronic acid. Standard deep dermal wounds were produced in vitro in human skin. The skin samples, with a central wound, were incubated in different culture media. Varying concentrations of amniotic fluid and amniotic fluid with added hyaluronidase were tested, and re-epithelialisation was assessed at 3, 7, and 12 days using light microscopy, after staining with haematoxylin and eosin. Amniotic fluid 50% resulted in a significantly higher (p < 0.05) grade of re-epithelialisation than Dulbecco's modified Eagle's medium and 10% amniotic fluid at all time points. When 50% amniotic fluid was compared with 10% foetal calf serum, no significant difference was found in grades of re-epithelialisation on days 3 and 12 and significantly higher grades of re-epithelialisation on day 7 (p < 0.05). Degradation of hyaluronic acid in the medium that contained 50% amniotic fluid gave significantly impaired re-epithelialisation (p < 0.05) on culture days 3 and 7. In conclusion, amniotic fluid promotes accelerated re-epithelialisation and hyaluronic acid is an important ingredient.
Foamy slag fundamentals and their practical application to electric furnace steelmaking (1999)

Using a mass-balance approach and isothermal saturation diagrams, steelmakers not only can monitor their existing slag practices, but design foamy slags to better suit their operations.
Effects of High-Flux Hemodialysis on Clinical Outcomes (2003)

Among the 1846 patients in the HEMO Study, chronic high-flux dialysis did not significantly affect the primary outcome of the all-cause mortality (ACM) rate or the main secondary composite outcomes, including the rates of first cardiac hospitalization or ACM, first infectious hospitalization or ACM, first 15% decrease in serum albumin levels or ACM, or all non-vascular access-related hospitalizations. The high-flux intervention, however, seemed to be associated with reduced risks of specific cardiac-related events. The relative risks (RR) for the high-flux arm, compared with the low-flux arm, were 0.80 [95% confidence interval (CI), 0.65 to 0.99] for cardiac death and 0.87 (95% CI, 0.76 to 1.00) for the composite of first cardiac hospitalization or cardiac death. Also, the effect of high-flux dialysis on ACM seemed to vary, depending on the duration of prior dialysis. This report presents secondary analyses to further explore the relationship between the flux intervention and the duration of dialysis with respect to various outcomes. The patients were stratified into a short-duration group and a long-duration group, on the basis of the mean duration of dialysis of 3.7 yr before randomization. In the subgroup that had been on dialysis for >3.7 yr, randomization to high-flux dialysis was associated with lower risks of ACM (RR, 0.68; 95% CI, 0.53 to 0.86; P = 0.001), the composite of first albumin level decrease or ACM (RR, 0.74; 95% CI, 0.60 to 0.91; P = 0.005), and cardiac deaths (RR, 0.63; 95% CI, 0.43 to 0.92; P = 0.016), compared with low-flux dialysis. No significant differences were observed in outcomes related to infection for either duration subgroup, however, and the trends for beneficial effects of high-flux dialysis on ACM rates were considerably weakened when the years of dialysis during the follow-up phase were combined with the prestudy years of dialysis in the analysis. For the subgroup of patients with <3.7 yr of dialysis before the study, assignment to high-flux dialysis had no significant effect on any of the examined clinical outcomes. These data suggest that high-flux dialysis might have a beneficial effect on cardiac outcomes. Because these results are derived from multiple statistical comparisons, however, they must be interpreted with caution. The subgroup results that demonstrate that patients with different durations of dialysis are affected differently by high-flux dialysis are interesting and require further study for confirmation.
Generation of Retinal Organoids with Mature Rods and Cones from Urine-Derived Human Induced Pluripotent Stem Cells (2018)

Urine cells, a body trash, have been successfully reprogrammed into human induced pluripotent stem cells (U-hiPSCs) which hold a huge promise in regenerative medicine. However, it is unknown whether or to what extent U-hiPSCs can generate retinal cells so far. With a modified retinal differentiation protocol without addition of retinoic acid (RA), our study revealed that U-hiPSCs were able to differentiate towards retinal fates and form 3D retinal organoids containing laminated neural retina with all retinal cell types located in proper layer as in vivo. More importantly, U-hiPSCs generated highly mature photoreceptors with all subtypes, even red/green cone-rich photoreceptors. Our data indicated that a supplement of RA to culture medium was not necessary for maturation and specification of U-hiPSC-derived photoreceptors at least in the niche of retinal organoids. The success of retinal differentiation with U-hiPSCs provides many opportunities in cell therapy, disease modeling, and drug screening, especially in personalized medicine of retinal diseases since urine cells can be noninvasively collected from patients and their relatives.
Plasma surface treatment on nylon fabrics by fluorocarbon compounds (1990)

Abstract Nylon fabrics were treated by low temperature fluorocarbon plasmas. All of the fluorocarbon plasmas applied altered the surface of nylon fabrics to be hydrophobic and water‐repellent. The durability of hydrophobicity and water repellency was examined by measurements of the water contact angle, the water droplet rolling‐off angle, and the breakthrough water pressure after plasma‐treated fabrics were washed. It was found that the structure of starting fluorocarbon and the plasma energy input were important factors in the durability. After washing, surface dynamics were investigated on different drying conditions. It was found that chemical composition and water repellency were dependent on drying conditions because of rotation of hydrophobic segments on the surface of fabrics.
Genome Sequence of Lactobacillus johnsonii PF01, Isolated from Piglet Feces (2011)

Lactobacillus johnsonii PF01, an autochthonous bacterium of the gastrointestinal tract, was isolated from a fecal sample from a piglet. The strain adhered specifically to the duodenal and jejunal epithelial cells of the piglet and had high bile resistance activity. Here we report the genomic sequence of L. johnsonii PF01.
Theoretical x-ray Bragg reflection widths and reflectivities of II-VI semiconductors (1992)

X-ray Bragg reflection widths and reflectivities are calculated by the dynamical scattering theory. Results on the binary II-VI semiconductors CdTe, HgTe, and the ternary alloys CdHgTe and CdZnTe are reported. For comparison, results on several III-V compounds are also reported. The dependence of the reflection widths and reflectivities on alloy composition, wavelength, layer thickness, and material properties are discussed. Especially in alloys, interesting effects, such as drastic changes of the reflectivity depending on the alloy composition, appear. The behavior of the widths and reflectivities can be understood by the examination of the competing processes of absorption and extinction. The results from calculated intrinsic reflex profiles are compared with approximation formulas.
On Color-, Infrared-, and Multimodal-Stereo Approaches to Pedestrian Detection (2007)

This paper presents an analysis of color-, infrared-, and multimodal-stereo approaches to pedestrian detection. We design a four-camera experimental testbed consisting of two color and two infrared cameras for capturing and analyzing various configuration permutations for pedestrian detection. We incorporate this four-camera system in a test vehicle and conduct comparative experiments of stereo-based approaches to obstacle detection using unimodal color and infrared imageries. A detailed analysis of the color and infrared features used to classify detected obstacles into pedestrian regions is used to motivate the development of a multimodal solution to pedestrian detection. We propose a multimodal trifocal framework consisting of a stereo pair of color cameras coupled with an infrared camera. We use this framework to combine multimodal-image features for pedestrian detection and to demonstrate that the detection performance is significantly higher when color, disparity, and infrared features are used together. This result motivates experiments and discussion toward achieving multimodal-feature combination using a single color and a single infrared camera arranged in a cross-spectral stereo pair. We demonstrate an approach to registering multiple objects across modalities and provide an experimental analysis that highlights issues and challenges of pursuing the cross-spectral approach to multimodal and multiperspective pedestrian analysis.
New lessons: classic treatments, expanding options in ulcerative colitis (2006)

Ulcerative colitis (UC) of moderate severity is a common presentation in gastroenterological practice and a number of treatment options exist to rapidly and effectively induce remission. This review highlights how novel formulations and dosing regimens can ensure treatment success at a greater convenience for the patient with no increased risk of adverse effects. 5-aminosalicyclic acid (5-ASA) is well tolerated with a low incidence of adverse effects, and has a significant role in the management of UC. Different formulations of oral 5-ASA are now available and allow targeted treatment to inflammatory areas of the small bowel and ascending colon. 5-ASA is often initiated at a low dose for patients with mild to moderately active UC and with increasing doses for those who fail or have a poor response. 5-ASA at high doses is increasingly being used as induction therapy for active UC, particularly in patients with recurrent and/or extensive disease. The recent ASCEND studies show that an induction dose of 5-ASA of 4.8 g daily in patients with moderate UC is significantly more effective and resolves symptoms faster compared with a daily dose of 2.4 g. The evidence provided by the ASCEND studies support the rationale for a 'top-down' dosing strategy for UC where more potent therapies are introduced at an earlier stage of moderately severe disease.
<title>End-to-end QoS support for adaptive applications over the Internet</title> (1998)

In current end systems, multiple flexible, complex and distributed applications concurrently share and compete both end system resource and transmission bandwidth of heterogeneous multi-protocol networks, especially the Internet. Our objective is to enable adaptation awareness in these applications to fully cope with the dynamics in resource availability over the heterogeneous Internet, as well as fluctuations in QoS requirements of the applications themselves. In this paper, we present the theoretical and practical aspects of a Task Control Model implemented in the middleware layer, which applies control theoretical approaches to utilize measurement-based samples monitored in the network traffic, as well as resource and QoS demand dynamics observed in the end systems.
Patient dose map indications on interventional X-ray systems and validation with Gafchromic XR-RV3 film (2014)

To help avoiding secondary effects of interventional procedures like skin damage, a dose map method has been developed to provide an indication of the local dose on a surface representative of individual patient shapes. To minimise user interactions, patient envelope shapes are automatically determined depending on simple patient data information. Local doses are calculated in 1-cm² areas depending on the estimated air kerma, table and gantry positions and system settings, taking into account the table and mattress attenuations and estimated backscatter from the patient. These local doses are cumulated for each location of the patient envelope during the clinical procedure. To assess the accuracy of the method, Gafchromic XR-RV3 films have been used in several operating configurations. Good visual agreements on cumulated dose localisation were obtained within the 1-cm² precision of the map and the dose values agreed within 24.9 % accuracy. The resulting dose map method has been integrated into GE Healthcare X-Ray angiographic systems and should help in the management of the dose by the users during the procedure.