gebhart/patrepeval huggingface dataset:
Molecular cloning and analysis of cDNA encoding a plant tryptophan decarboxylase: comparison with animal dopa decarboxylases. (1989)

The sequence of a cDNA clone that includes the complete coding region of tryptophan decarboxylase (EC 4.1.1.28, formerly EC 4.1.1.27) from periwinkle (Catharanthus roseus) is reported. The cDNA clone (1747 base pairs) was isolated by antibody screening of a cDNA expression library produced from poly(A)+ RNA found in developing seedlings of C. roseus. The clone hybridized to a 1.8-kilobase mRNA from developing seedlings and from young leaves of mature plants. The identity of the clone was confirmed when extracts of transformed Escherichia coli expressed a protein containing tryptophan decarboxylase enzyme activity. The tryptophan decarboxylase cDNA clone encodes a protein of 500 amino acids with a calculated molecular mass of 56,142 Da. The amino acid sequence shows a high degree of similarity with the aromatic L-amino acid decarboxylase (dopa decarboxylase) and the alpha-methyldopa-hypersensitive protein of Drosophila melanogaster. The tryptophan decarboxylase sequence also showed significant similarity to feline glutamate decarboxylase and mouse ornithine decarboxylase, suggesting a possible evolutionary link between these amino acid decarboxylases.
<title>MMIC: a key technology for future communications satellite antennas</title> (1991)

The results of ongoing development programs in the area of advanced satellite antenna technology currently being pursued at COMSAT Laboratories, under the sponsorship of the World Systems Division of COMSAT Corporation, are described. These programs are exploiting the promise of monolithic microwave integrated circuits (MMICs) as they apply to active phased arrays. Performance data on two phased-array programs are presented. The first program involves the development of a 64-element array in which MMICs are used to optimize and reconfigure the radiation pattern performance. The array is capable of generating a single beam and accurately synthesizing a specified radiation performance. The second array program, building on knowledge gained in the first program, addresses the problems of generating multiple beams, as well as the effect of power amplifiers on multicarrier performance. MMICs are employed in the beam-forming matrix and in the distributed 2-W solid-state power amplifiers. This design is capable of forming four simultaneous, independently steerable beams while allowing for flexible power sharing among the beams.© (1991) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
A method for on-line reorganization of a database (1997)

Any database management system may need some type of reorganization. However, reorganization typically requires taking a database off line, which can be unacceptable for a very large or highly available (24-hour) database. A solution is to reorganize on line (concurrently with users' reading and writing of data in the database). This paper describes a method for performing one type of reorganization on line. The type of reorganization distributes free space evenly, removes overflow, and clusters data. The method for on-line reorganization copies data while arranging the data in the new copy in reorganized form. The method then applies the database log to bring the new copy up to date (to reflect users' writing of the old copy). The method maintains a table that maps between old and new record identifiers, to match log entries with data records in the new copy.
Large‐scale purification and characterization of non‐glycosylated Gc globulin (vitamin D‐binding protein) from plasma fraction IV (2006)

Gc globulin, also called vitamin D-binding protein, is a plasma protein involved in the extracellular actin-scavenger system. Low levels of Gc globulin have been found to correlate with multiple organ failure and nonsurvival of patients with fulminant hepatic failure and trauma. Therefore substitution therapy with Gc globulin might be beneficial for such patients, increasing their chance of survival. In the present study, we describe a large-scale purification process for the production of a virus-safe human plasma-derived Gc globulin from Cohn fraction IV paste. The process includes three ion-exchange-chromatography steps, followed by a gel filtration, and two virus-reduction steps are implemented. The Gc globulin product was characterized with respect to purity, functional activity, glycosylation and, finally, with respect to content of endotoxin. From the results, it can be concluded that human Gc globulin purified from Cohn fraction IV is non-glycosylated. The purified Gc globulin is able to mask the presence of endotoxin by 20%.
In Vivo Bioluminescence Imaging as a Tool for Drug Development (2014)

Plasmons are quanta associated with longitudinal waves propagating in matter through the collective motion of large numbers of electrons. e origin of the enormous Raman enhancement is produced by at least two main mechanisms that contribute to the surface-enhanced Raman scattering (SERS) eect: (1) an electromagnetic (EM) eect occurring near metal surface structures associated with large local elds caused by EM resonances, oen referred to as surface plasmons, and (2) a chemical eect involving a scattering process associated with chemical interactions between the molecule and the metal surface. When a metallic nanostructured surface is irradiated by an incident EM eld (e.g., a laser beam), conduction electrons are displaced into frequency oscillations equal to those of the incident light. ese oscillating electrons, called surface plasmons, produce a secondary electric eld, which adds to the incident eld. ese elds can be quite large (106-to 107-fold, or even up to 1015-fold enhancement at hot spots). When these oscillating electrons become spatially conned, as is the case for isolated metallic nanospheres or otherwise roughened metallic surfaces (nanostructures), there is a characteristic frequency (the plasmon frequency) at which there is a resonant response of the collective oscillations to the incident eld. is condition yields intense localized elds that can interact with molecules in contact with or near the metal surface [1-4]. In an eect analogous to a lightning rod eect, secondary elds can become concentrated at high curvature points on the roughened metal surface.
KlebSeq: A Diagnostic Tool for Healthcare Surveillance and Antimicrobial Resistance Monitoring of <i>Klebsiella pneumoniae</i> (2016)

ABSTRACT Healthcare-acquired infections (HAIs) kill tens of thousands of people each year and add significantly to healthcare costs. Multidrug resistant and epidemic strains are a large proportion of HAI agents, and multidrug resistant strains of Klebsiella pneumoniae , a leading HAI agent, have become an urgent public health crisis. In the healthcare environment, patient colonization of K. pneumoniae precedes infection, and transmission via colonization leads to outbreaks. Periodic patient screening for K. pneumoniae colonization has cost-effective and life-saving potential. In this study, we describe the design and validation of KlebSeq, a highly informative screening tool that detects Klebsiella species and identifies clinically important strains and characteristics using highly multiplexed amplicon sequencing without a live culturing step. We demonstrate the utility of this tool on several complex specimen types including urine, wound swabs and tissue, several types of respiratory, and fecal, showing K. pneumoniae species and clonal group identification and antimicrobial resistance and virulence profiling, including capsule typing. Use of this amplicon sequencing tool can be used to screen patients for K. pneumoniae carriage to assess risk of infection and outbreak potential, and the expansion of this tool can be used for several other HAI agents or applications.
Detecting similar documents using salient terms (2002)

We describe a system for rapidly determining document similarity among a set of documents obtained from an information retrieval (IR) system. We obtain a ranked list of the most important terms in each document using a rapid phrase recognizer system. We store these in a database and compute document similarity using a simple database query. If the number of terms found to not be contained in both documents is less than some predetermined threshold compared to the total number of terms in the document, these documents are determined to be very similar. We compare this to the shingles approach.
Fatigue strength improvement of box welds using low transformation temperature welding material. Tripled fatigue strength by post weld heat treatment (2002)

The fatigue strength of box welds was tripled by using low transformation temperature welding wire and postweld heat treatment (PWHT). The improved fatigue strength was obtained due to the stress ratio effect resulting from compressive residual stress. The low transformation temperature welding material which contains 10 mass % Cr and 10 mass % Ni expands during the final cooling process of welding. This expansion of weld metal induced the compressive residual stress around the welded part. The PWHT at 720°C for 1 hour and subsequent cooling in air induced the transformation from austenite to martensite at the same time for three passes. That is, the volume expansion on three passes by PWHT became large compare with that on final pass in as-welded condition, and the compressive residual stress could be induced efficiently.
Real-Time Three-Dimensional Microwave Monitoring of Interstitial Thermal Therapy (2017)

We report a method for real-time three-dimensional monitoring of thermal therapy through the use of noncontact microwave imaging. This method is predicated on using microwaves to image changes in the dielectric properties of tissue with changing temperature. Instead of the precomputed linear Born approximation that was used in prior work to speed up the frame-to-frame inversions, here we use the nonlinear distorted Born iterative method (DBIM) to solve the electric volume integral equation (VIE) to image the temperature change. This is made possible by using a recently developed graphic processing unit accelerated conformal finite difference time domain method to solve the forward problem and update the electric field in the monitored region in each DBIM iteration. Compared to our previous work, this approach provides a far superior approximation of the electric field within the VIE, and thus yields a more accurate reconstruction of tissue temperature change. The proposed method is validated using a realistic numerical model of interstitial thermal therapy for a deep-seated brain lesion. With the new DBIM, we reduced the average estimation error of the mean temperature within the region of interest from 2.5 to 1.0 for the noise-free case, and from 2.9 to 1.7 for the 2% background noise case.
WT1 as a Novel Target Antigen for Cancer Immunotherapy (2002)

Wild-type Wilms' tumor gene WT1 is expressed at high levels not only in most of acute myelocytic, acute lymphocytic, and chronic myelocytic leukemia, but also in various types of solid tumors including lung cancer. We tested the ability of the gene product (WT1) to serve as a target antigen for tumor specific immunotherapy both in human in vitro system and mouse in vivo system. In the latter, we can evaluate the efficacy and the side effects of WT1 vaccination in vivo. In the human in vitro system, two WT1 peptides that contain HLA-A2.1 binding anchor motifs were determined to bind to HLA-A2.1 molecules. Peripheral blood mononuclear cells (PBMC) from an HLA-A2.1-psitive donor were repeatedly stimulated in vitro with TAP-deficient T2 cells pulsed with each of these two peptides, and CD8-positive cytotoxic T lymphocytes (CTLs) that specifically lyse WT1-expressing, HLA-A2.1-positive tumor cells were induced. Other groups also have succeeded in generating CTLs which specifically lyse WT1-expressing leukemia cells, and which do not inhibit colony-formation of normal hematopoietic cells that express WT1 at physiological levels. In the mouse in vivo system, immunization of C57BL/6 mice with one WT1 peptide with relatively high binding affinity for H-2D(b) molecules, which contain H-2D(b) binding anchor motifs, induced CTLs, which specifically lysed WT1-expressing tumor cells in an H-2D(b)-restricted manner. Furthermore, mice immunized with the WT1 peptide (peptide vaccination) or WT1 cDNA (DNA vaccination) rejected challenges by WT1-expressing tumor cells and survived with no signs of auto-aggression to WT1-expressing normal organs by the induced CTLs. The WT1 protein has been identified as a novel tumor antigen and recent investigations provide a rationale for developing WT1-based adoptive T cell therapy and vaccination against various kinds of malignant neoplasms.
A continuous folding process for sheet materials (2004)

In this paper, we present a new and innovative sheet material folding technology and the associated advances in folding different patterns using continuous manufacturing techniques. A novel approach is developed for the continuous folding process where sheet material is progressively folded in two dimensions, through a set of rollers, followed by a configured roller for the final folding in the third dimension. The final roller can be designed for longitudinal folding, cross-folding and angular folding to produce the desired folded pattern. This process is more economical than the traditional forming processes. An application of this process to the production of impact energy absorption structures is presented.
3D graphics for everyday communication (2004)

It is still difficult for casual users to author 3D models from scratch. Our goal is to design easy-to-use 3D graphics authoring tools so that people can use 3D graphics as an everyday communication tool such as word processors and emails. This paper introduces some of our projects toward the goal. To be specific, we introduce a sketch-based modeling system, a 3D painting system, a 3D sketching system with automatic suggestions, and a clothing manipulation technique.
Enabling Droplet Functionality on Anisotropic Ratchet Conveyors (2017)

Anisotropic ratchet conveyors (ARCs) are a recently developed microfluidic platform that transports liquid droplets through a passive, microfabricated surface pattern and applied orthogonal vibrations. In this work, three new functionalities are presented for controlling droplet transport on the ARC system. These devices can pause droplet transport (ARC gate), decide between two pathways of droplet transport (ARC switch), and pass droplets between transport tracks (ARC delivery junction). All devices function solely through the modification of pinning forces acting on the transported droplet and are the first reported devices that can selectively control droplet timing and directionality without active (e.g., thermal, electrical, or magnetic) surface components.
Chemoselective Sequential “Click” Ligation Using Unsymmetrical Bisazides (2012)

Unsymmetrical bisazides containing chelating and nonchelating azido groups undergo chemoselective three-component copper(I)-catalyzed azide-alkyne conjugation reactions with two different alkyne molecules. In conjunction with the reactivity gap between aromatic and aliphatic alkynes, a bistriazole molecule can be generated with excellent regioselectivity by mixing two alkynes and a bisazide in a single reaction container. This method is applicable in aqueous solutions at neutral pH, which may lend utilities in bioconjugation applications.
FPGA implementation of neighborhood-of-four cellular automata random number generators (2002)

Random number generators (RNGs) based upon neighborhood-of-four cellular automata (CA) with asymmetrical, non-local connections are explored. A number of RNGs that pass Marsaglia's rigorous Diehard suite of random number tests have been discovered. A neighborhood size of four allows a single CA cell to be implemented with a four-input lookup table and a one-bit register which are common building blocks in popular field programmable gate arrays (FPGAs). The investigated networks all had periodic (wrap around) boundary conditions with either 1-d, 2-d, or 3-d interconnection topologies. Trial designs of 64-bit networks using a Xilinx XCV1000-6 FPGA predict a maximum clock rate of 214 MHz to 230 MHz depending upon interconnection topology.
Business Process Simulation with HP Process Manager (2001)

workflow, simulation Business processes present business logic of enterprises and or web services. In current Internet based dynamic business environment, business logic and resource allocation may have to change more frequently than before to match various business conditions in time. Business process deployment is a very costly procedure. It is desirable that a business process reengineering action could be verified before it is deployed. In this paper, we present an innovative business process simulation system that can use historical execution information of business processes when it is available as reference to set up simulation conditions. Integrated with a process modeling facility, this simulation system allows business processes to be simulated before they are deployed.
Improving the selective cancer killing ability of ZnO nanoparticles using Fe doping (2011)

This work reports a new method to improve our recent demonstration of zinc oxide (ZnO) nanoparticles (NPs) selectively killing certain human cancer cells, achieved by incorporating Fe ions into the NPs. Thoroughly characterized cationic ZnO NPs (∼6 nm) doped with Fe ions (Zn(1-x )Fe (x) O, x = 0-0.15) were used in this work, applied at a concentration of 24 μg/ml. Cytotoxicity studies using flow cytometry on Jurkat leukemic cancer cells show cell viability drops from about 43% for undoped ZnO NPs to 15% for ZnO NPs doped with 7.5% Fe. However, the trend reverses and cell viability increases with higher Fe concentrations. The non-immortalized human T cells are markedly more resistant to Fe-doped ZnO NPs than cancerous T cells, confirming that Fe-doped samples still maintain selective toxicity to cancer cells. Pure iron oxide samples displayed no appreciable toxicity. Reactive oxygen species generated with NP introduction to cells increased with increasing Fe up to 7.5% and decreased for >7.5% doping.
Characterization of overactive bladder in women in a primary care setting (2011)

OAB was evident in 44% of all female patients surveyed, which is much higher than previously reported estimates. In addition, overweight women were more likely to have OAB. Increased awareness of OAB in the primary care setting should be considered for women's general health.
Clutter-Suppressed, Subsurface Interferometric SAR Imaging of Target Under Slightly Rough Surface (2018)

A subsurface interferometric synthetic aperture radar (InSAR) imaging technique is proposed for the detection and localization of underground facilities in the presence of a rough ground surface. The new approach consists of a two-step procedure: first, surface clutter suppression is performed with a polarimetric difference operation that does not alter the propagation phase of the target scattered signal; then a subsurface interferometric algorithm is applied to infer target depth by correlating the clutter-suppressed images obtained at two observation angles. The performance of the overall sensing algorithm for scene depth profile mapping from an airborne system is demonstrated with full-wave simulation data.
Cation-radical catalyzed Diels-Alder reaction (1981)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCation-radical catalyzed Diels-Alder reactionDennis J. Bellville, David W. Wirth, and Nathan L. BauldCite this: J. Am. Chem. Soc. 1981, 103, 3, 718–720Publication Date (Print):February 1, 1981Publication History Published online1 May 2002Published inissue 1 February 1981https://pubs.acs.org/doi/10.1021/ja00393a061https://doi.org/10.1021/ja00393a061research-articleACS PublicationsRequest reuse permissionsArticle Views2116Altmetric-Citations201LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Smart Cement Piezoresistivity Characterization with Sodium Metasilicate under Temperature and Curing Environments for Oil Well–Cementing (2016)

The smart cement behavior with 0.3% sodium metasilicate (SMS) at 80°C (176°F) under two different environments was investigated in this study. The smart cement was made using the class H oil well cement and 0.1% conductive filler to make it a bulk chemo-piezoresistive material with highly sensing property. The smart cement was cured in air and also submerged in water-saturated sand at 80°C up to 28 days. The smart cement initial resistivity (ρo) decreased from 0.97 to 0.87 Ω·m with 0.3% SMS, a 10% decrease. Similarly the minimum resistivity (ρmin) decreased from 0.81 to 0.72 Ω·m with 0.3% SMS, an 11% decrease, which is an indication of the chemo-resistivity of the smart cement. The resistivity changes were higher than the unit weight changes in the smart material. The resistivity of the smart material oven cured in saturated sand at 80°C was 55 to 75% less than the resistivity of the material cured at 80°C after curing for one, seven, and 28 days. The material resistivity with 0.3% SMS cured in both conditions were about 25–55% less than the smart cement only. The resistivity with curing time under different curing conditions was modeled using a nonlinear curing model, and the prediction agreed well with experimental results. Also, a nonlinear power relationship was used to relate the resistivity to the weight change in the cement. The smart cement cured at high temperature (80°C) showed piezoresistive response under applied stress. For the smart cement the piezoresistivity at compressive strength changed from 245 to 475%. With 0.3% SMS the chemo-piezoresistivity at compressive strength varied from 160 to 345% based on the curing time and curing environment, which is an indication of the chemo-piezoresistivity of the smart cement. The smart cement cured in saturated sand showed higher piezoresistivity, about 15–25% more compared to the dry curing. Since the failure strain for the smart cement was only 0.2%, the piezoresistivity at peak compressive stress of the smart cement has been enhanced by more than 900 times, making it highly sensing. The compressive stress resistivity constitutive model predicated the material behavior very well. Also, a hyperbolic model was used to predict correlation between materials properties and also changes with curing time investigated in this study.
Synthesis of Chiral Tetrasubstituted Azetidines from Donor–Acceptor Azetines via Asymmetric Copper(I)‐Catalyzed Imido‐Ylide [3+1]‐Cycloaddition with Metallo‐Enolcarbenes (2019)

Abstract The all‐ cis stereoisomers of tetrasubstituted azetidine‐2‐carboxylic acids and derivatives that possess three chiral centers have been prepared in high yield and stereocontrol from silyl‐protected Z‐γ‐substituted enoldiazoacetates and imido‐sulfur ylides by asymmetric [3+1]‐cycloaddition using chiral sabox copper(I) catalysis followed by Pd/C catalytic hydrogenation. Hydrogenation of the chiral p ‐methoxybenzyl azetine‐2‐carboxylates occurs with both hydrogen addition to the C=C bond and hydrogenolysis of the ester.