gebhart/patrepeval huggingface dataset:
Cometabolism of a Nongrowth Substrate:<scp>l</scp>-Serine Utilization by<i>Corynebacterium glutamicum</i> (2004)

Despite its key position in central metabolism, L-serine does not support the growth of Corynebacterium glutamicum. Nevertheless, during growth on glucose, L-serine is consumed at rates up to 19.4 +/- 4.0 nmol min(-1) (mg [dry weight])(-1), resulting in the complete consumption of 100 mM L-serine in the presence of 100 mM glucose and an increased growth yield of about 20%. Use of 13C-labeled L-serine and analysis of cellularly derived metabolites by nuclear magnetic resonance spectroscopy revealed that the carbon skeleton of L-serine is mainly converted to pyruvate-derived metabolites such as L-alanine. The sdaA gene was identified in the genome of C. glutamicum, and overexpression of sdaA resulted in (i) functional L-serine dehydratase (L-SerDH) activity, and therefore conversion of L-serine to pyruvate, and (ii) growth of the recombinant strain on L-serine as the single substrate. In contrast, deletion of sdaA decreased the L-serine cometabolism rate with glucose by 47% but still resulted in degradation of L-serine to pyruvate. Cystathionine beta-lyase was additionally found to convert L-serine to pyruvate, and the respective metC gene was induced 2.4-fold under high internal L-serine concentrations. Upon sdaA overexpression, the growth rate on glucose is reduced 36% from that of the wild type, illustrating that even with glucose as a single substrate, intracellular L-serine conversion to pyruvate might occur, although probably the weak affinity of L-SerDH (apparent Km, 11 mM) prevents substantial L-serine degradation.
Mixed-metal substrates for applications in metal-enhanced fluorescence (2011)

Over the last decade Metal-Enhanced Fluorescence (MEF) has emerged as the next generation of fluorescence spectroscopy, i.e. near-field fluorescence. However, in contrast to our collective knowledge and understanding of classical far-field fluorescence, we know relatively little. MEF is a consequence of the near-field interactions of fluorophores (dipoles) with the surface plasmons generated in plasmon supporting materials, where the optical properties of the metal afford for a wavelength dependence of MEF. In this paper we show that we are not limited to the properties of the individual metals for MEF, but in fact, surface deposits of mixed metals can create new dephased plasmon resonance bands, not present in the individual metals themselves. Subsequently, mixed metal substrates (MMS) offer significant opportunities for the multifarious and forever growing applications of MEF.
Nocturnal overdrive pacing reduces circulation delay (2005)

Sleep apnea affects 24% of men and 9% of women. Circulation delay (CD) is the time from end of an apnea ro oxygen saturation nadir. To evaluafe efleects of cardiac pacing on sleep apnea, I9 patients were enrolled in a WO center, single blind, randomized crossover study comparing Nocturnal Overdrive Pacing (NOP, 7.5 bpm lower night rate) to Control (45 bpm lower night rate). Patients underwent two sleep studies measuring Apnea- Hypupnea Index (AHI, [# (apneas+hypopneus)/hour of sleep] and finger oxygen saturation. CD WAS measured with an adaptive window in 14 patients. NUP improved AHI 210 eventshour in 4 {Responders), and not in 10 (Non-responders). NUP decreased CD in all patients. CD was 25.633.5 sec, Control, and 20.9H.7 sec, NUP, (p=0.03). Changes in CD were not different in Responders YS. Non-responders (4.8G.O sec vs. 4.6G.9 sec, p=0.9). NUP reduced CD, however, CD did not predict relative changes in severity of sleep apnea as measured by AHI.
Field Efficacy of<i>Dactylaria higginsii</i>as a Bioherbicide for the Control of Purple Nutsedge (<i>Cyperus rotundus</i>)<sup>1</sup> (2000)

An isolate of the fungus Dactylaria higginsii obtained from purple nutsedge in Florida was highly pathogenic to Cyperus spp. The potential of this isolate as a bioherbicide was field tested in natural populations of purple nutsedge in Gainesville and Jay, FL. The fungus was applied in 0.5% Metamucil® as a carrier, and the treatments were: carrier only, 105 conidia/ml carrier, and 106 conidia/ml carrier. Treatments were applied as single, double, or triple postemergence (POST) sprays at biweekly intervals. The disease and secondary infections developed in about 5 and 15 d after inoculation, respectively, killing most of the infected leaves. All weed growth parameters and disease progress rates were affected by inoculum dosage and inoculation frequencies. Three inoculations, each at 106 conidia/ml, provided effective control of purple nutsedge compared to a single inoculation, as measured by shoot dry weight, tuber numbers, and tuber dry weight. Higher rates of disease progress and disease levels, defined by the area under the disease progress curve (AUDPC), occurred with three inoculations at 106 conidia/ml. Disease progress was slower and the level of weed control was lower at 105 conidia/ml compared to the higher inoculum level. Three applications of 106 conidia/ml provided >90% nutsedge control. Dactylaria higginsii appears to be an effective bioherbicide candidate deserving further development for commercial use.Nomenclature: Purple nutsedge, Cyperus rotundus L., #3 CYPRO, Dactylaria, D. higginsii (Luttrell) M. B. Ellis.Additional index words: Mycoherbicide, biological control of weeds, fungal pathogen.Abbreviations: AUDPC, area under the disease progress curve; PDA, potato dextrose agar; POST, postemergence; rG, disease progress.
Microstructure-fiber-based ultrafast optical parametric oscillators (2007)

We report on the generation ultrafast pulses using a microstructure-fiber-based optical parametric oscillators. This approach provides new opportunities for extending the functionality of mode-locked fiber lasers.
Spar concrete monolithic design for offshore wind turbines (2016)

Significant research efforts are now being directed at floating offshore wind turbines. The main challenge posed by floating wind turbines is the high construction and installation costs of the substructure, which make it too expensive for commercial exploitation in the current energy market. With the aim of achieving a cost-effective floating platform for offshore wind turbines, a new concept of a monolithic floating spar buoy is hereby presented. The monolithic concrete structure includes both the tower and the floater, built as a continuous single piece. This new concept offers a significant cost reduction during the construction phase and also while in operation, because the platform becomes almost free of maintenance during its lifetime. The main dimensions and the hydrostatic and hydrodynamic properties are presented, including a basic structural assessment of the platform to ensure its structural integrity. The construction and installation processes are presented, taking into account the special requirements of the monolithic design. Finally, a cost comparison between a steel and an equivalent concrete platform design has been performed, showing a material cost reduction larger than 60% in the case of the concrete design.
Optical properties of animal tissue as diabetes progresses (2006)

DPDW methodology in the near infrared was used to calculate absorption and scattering in wounds of healthy and diabetic rats. Differences observed as diabetes progresses can be correlated to the delayed healing observed in diabetics.
Adenovirus-Mediated Utrophin Gene Transfer Mitigates the Dystrophic Phenotype of mdx Mouse Muscles (1999)

Utrophin is a close homolog of dystrophin, the protein whose mutations cause Duchenne muscular dystrophy (DMD). Utrophin is present at low levels in normal and dystrophic muscle, whereas dystrophin is largely absent in DMD. In such cases, the replacement of dystrophin using a utrophin gene transfer strategy could be more advantageous because utrophin would not be a neoantigen. To establish if adenovirus (AV)-mediated utrophin gene transfer is a possible option for the treatment of DMD, an AV vector expressing a shortened version of utrophin (AdCMV-Utr) was constructed. The effect of utrophin overexpression was investigated following intramuscular injection of this AV into mdx mice, the mouse model of DMD. When the tibialis anterior (TA) muscles of 3- to 5-day-old animals were injected with 5 microl of AdCMV-Utr (7.0 x 10(11) virus/ml), an average of 32% of fibers were transduced and the transduction level remained stable for at least 60 days. The presence of utrophin restored the normal histochemical pattern of the dystrophin-associated protein complex at the cell surface and resulted in a reduction in the number of centrally nucleated fibers. The transduced fibers were largely impermeable to the tracer dye Evans blue, suggesting that utrophin protects the surface membrane from breakage. In vitro measurements of the force decline in response to high-stress eccentric contractions demonstrated that the muscles overexpressing utrophin were more resistant to mechanical stress-induced injury. Taken together, these data indicate that AV-mediated utrophin gene transfer can correct various aspects of the dystrophic phenotype. However, a progressive reduction in the number of transduced fibers was observed when the TA muscles of 30- to 45-day-old mice were injected with 25 microl of AdCMV-Utr. This reduction coincides with a humoral response to the AV and transgene, which consists of a hybrid mouse-human cDNA.
LpMab-23: A Cancer-Specific Monoclonal Antibody Against Human Podoplanin (2017)

Human podoplanin (hPDPN), the ligand of C-type lectin-like receptor-2, is involved in cancer metastasis. Until now, many monoclonal antibodies (mAbs) have been established against hPDPN. However, it is still difficult to develop a cancer-specific mAb (CasMab) against hPDPN because the protein sequence of hPDPN expressed in cancer cells is the same as that in normal cells. Herein, we report LpMab-23 of the mouse IgG<sub>1</sub> subclass, a novel CasMab against hPDPN. In an immunohistochemical analysis, LpMab-23 reacted with tumor cells of human oral cancer, but did not react with normal cells such as lymphatic endothelial cells (LECs). In contrast, LpMab-17, another anti-hPDPN mAb, reacted with both tumor cells and LECs. Furthermore, flow cytometric analysis revealed that LpMab-23 reacted with hPDPN-expressing cancer cell lines (LN319, RERF-LC-AI/hPDPN, Y-MESO-14/hPDPN, and HSC3/hPDPN) but showed little reaction with normal cells (LECs and HEK-293T), although another anti-hPDPN mAb, LpMab-7, reacted with both hPDPN-expressing cancer cells and normal cells, indicating that LpMab-23 is a CasMab against hPDPN.
Magnetically driven plasmid DNA delivery with biodegradable polymeric nanoparticles (2007)

Targeting gene therapy remains a challenge. The use of magnetic force to achieve this was investigated in the present study. It was hypothesized that nanoparticles with both controllable particle size and magnetic properties would enable magnetically driven gene delivery. We investigated this hypothesis by creating a family of novel biodegradable polymeric superparamagnetic nanoparticle (MNP) formulations. Polylactide MNP were formulated using a modified emulsification-solvent evaporation methodology with both the incorporation of oleate-coated iron oxide and a polyethylenimine (PEI) oleate ion-pair surface modification for DNA binding. MNP size could be controlled by varying the proportion of the tetrahydrofuran cosolvent. Magnetically driven MNP-mediated gene transfer was studied using a green fluorescent protein reporter plasmid in cultured arterial smooth muscle cells and endothelial cells. MNP-DNA internalization and trafficking were examined by confocal microscopy. Cell growth inhibition after MNP-mediated adiponectin plasmid transfection was studied as an example of a therapeutic end point. MNP-DNA complexes protected DNA from degradation and efficiently transfected quiescent cells under both low and high serum conditions after a 15 min exposure to a magnetic field (500 G). There was negligible transfection with MNP in the absence of a magnetic field. Larger sized MNP (375 nm diameter) exhibited higher transfection rates compared with 185 nm- and 240 nm-sized MNP. Internalized larger sized MNP escaped lysosomal localization and released DNA in the perinuclear zone. Adiponectin plasmid DNA delivery using MNP resulted in a dose-dependent growth inhibition of cultured arterial smooth muscle cells. It is concluded that magnetically driven plasmid DNA delivery can be achieved using biodegradable MNP containing oleate-coated magnetite and surface modified with PEI oleate ion-pair complexes that enable DNA binding.
Energetic ion mass spectrometer (1982)

A new type of spectrometer for space application has been developed and tested in the laboratory which measures separately, mass/charge and the three-dimensional velocity distribution of incident ions. The instrument has relatively high resolution (m/Δm = 600 amu/m) and allows rapid measurement of the four-dimensional set: mass/charge, speed, elevation angle, and azimuth angle. The basic components include: (1) a sector magnet which serves as a momentum/charge filter with a very wide angular acceptance, (2) an electrostatic deflector which spreads the momentum-analyzed ion beam according to energy/charge (or mass/charge), (3) microchannel plate detectors with position sensitive readout, and (4) a set of grids with variable applied voltage to accelerate or decelerate the incident ions before they enter the analysis part of the instrument. For application in a comet environment an electrostatic mirror would be used to reflect ions into the instrument while preventing dust from entering the field of view.
Fine mapping of a male sterility gene ms-3 in a novel cucumber (Cucumis sativus L.) mutant (2017)

The cucumber male sterility gene ms - 3 was fine mapped in a 76 kb region harboring an MMD1 -like gene Csa3M006660 that may be responsible for the male sterile in cucumber. A cucumber (Cucumis sativus L.) male sterile mutant (ms-3) in an advanced-generation inbred line was identified, and genetic analysis revealed that the male sterility trait was controlled by a recessive nuclear gene, ms-3, which was stably inherited. Histological studies suggested that the main cause of the male sterility was defective microsporogenesis, resulting in no tetrad or microspores being formed. Bulked segregant analysis (BSA) and genotyping of an F<sub>2</sub> population of 2553 individuals were employed used to fine map ms-3, which was delimited to a 76 Kb region. In this region, a single non-synonymous SNP was found in the Csa3M006660 gene locus, which was predicted to result in an amino acid change. Quantitative RT-PCR analysis of Csa3M006660 was consistent with the fact that it plays a role in the early development of cucumber pollen. The protein encoded by Csa3M006660 is predicted to be homeodomain (PHD) finger protein, and the high degree of sequence conservation with homologs from a range of plant species further suggested the importance of the ms-3 non-synonymous mutation. The data presented here provide support for Csa3M006660 as the most likely candidate gene for Ms-3.
Multi‐arm star polyisobutylenes: 3. New insight into the chemistry of star formation: Star‐star coupling and core contraction (1996)

Abstract Details of the chemistry of linking living polyisobutylene (PIB⊕) pre‐arms with divinylbenzene (DVB) to multi‐arm PIB stars have been studied by triple‐detector size‐exclusion chromatography (SEC). Computer‐aided deconvolution of multimodal differential refractive index (RI), laser light scattering (LLS), and ultraviolet (UV) traces obtained by SEC indicates the formation of complex product mixtures. The observations were interpreted in terms of inter‐and intramolecular reactions of initially‐formed active stars. Thus intermolecular or star‐star couplings are proposed to lead to higher order (secondary, tertiary, etc.) stars, whereas intramolecular reactions result in core‐contraction. The mechanism of these reactions has been outlined. The effect of PIB⊕ pre‐arm length on star‐star coupling was analyzed.
Photoelectrochemical cells - Conversion of intense optical energy (1976)

Conversion of optical energy to chemical energy and/or electrical energy using wet photoelectrochemical cells is described. Emphasis is on (1) the photoelectrolysis of H2O to H2 and O2 using cells having n-type semiconductor photoelectrodes fabricated from TiO2, SnO2, SrTiO3, KTaO3, and KTa(0.77)Nb(0.23)O3, and (2) the conversion of light to electrical energy using CdSe- and CdS-based cells with polysulfide electrolytes.
Enhanced ferroelectric properties in laser-ablated SrBi2Nb2O9 thin films on platinized silicon substrate (2002)

Non-c-axis-oriented SrBi2Nb2O9 (SBN) thin films were grown on Pt/TiO2/SiO2/Si substrates using the pulsed-laser-deposition technique. X-ray diffraction results confirmed the textured growth of SBN thin films along (115) and (200) orientations. The increase in the value of the dielectric permittivity and decrease in the tangential loss of the films with an increase in annealing temperature were attributed to grain size dependence. SBN thin films annealed at 750 °C exhibited a maximum value of the dielectric constant of ∼346 with a dissipation factor of 0.02. Thin films with certain deposition parameters exhibited the highest remanent polarization (Pr) and coercive field, 25.7 μC/cm2 and 198 kV/cm, respectively. There was minimal (&lt;20%) degradation in the switchable polarization (P*−P∧) after 1010 switching cycles. At lower field, the leakage current follows ohmic behavior, and at higher field, up to 100 kV/cm, the leakage current density was about 5×10−7 A/cm2.
Time Delay Estimation Via Wasserstein Distance Minimization (2019)

Time delay estimation between signals propagating through nonlinear media is an important problem with application to radar, underwater acoustics, damage detection, and communications (to name a few). Here, we describe a simple approach for determining the time delay between two such signals via minimization of the one-dimensional Wasserstein distance. The solution can be computed efficiently digitally in O(N), or in linear time in circuitry. We demonstrate the approach in the estimation of time delay between acoustic (Lamb) waves generated in an aluminum plate, and compare to alternative approaches.
Improving Solubility and Pharmacokinetics of Meloxicam via Multiple-Component Crystal Formation (2012)

Meloxicam is a nonsteroidal anti-inflammatory drug prescribed for rheumatoid arthritis, osteoarthritis, postoperative pain and fever. Meloxicam exhibits low solubility in acidic aqueous media and a slow onset of action in biological subjects. An oral dosage form of meloxicam with enhanced aqueous solubility is desired to enable a faster onset of action and its use for mild-to-medium-level acute pain relief. With this in mind, we examine the solubility and pharmacokinetics of 12 meloxicam cocrystals with carboxylic acids. Dissolution studies of meloxicam and its cocrystals were performed in pH 6.5 phosphate buffer solutions at 37 °C. In addition, pharmacokinetic profiles over four hours were acquired after oral administration of a 10 mg/kg (meloxicam equivalent) solid suspension in rats. The majority of meloxicam cocrystals were found to achieve higher meloxicam concentrations in dissolution media and enhanced oral absorption compared to that of pure meloxicam. All meloxicam cocrystals were converted to meloxicam form I when the slurry reached equilibrium. To better understand how cocrystallization impacts the absorption of meloxicam after oral administration, correlations between the in vitro and in vivo data were explored. The results suggest that the meloxicam cocrystals with a faster dissolution rate would exhibit increased oral absorption and an earlier onset of action.
Guided Target-Oriented 3D VSP Survey Design (2011)

In many situations, 3D VSP data are acquired to improve subsurface interpretation based on surface seismic data. However, this surface seismic prior information is often underutilized when designing the 3D VSP surveys to acquire. A Bayesian methodology is described for designing experiments or surveys that will optimally complement all previously available information. This methodology uses strong prior information to linearize the problem, and to guide the design toward maximally reducing forecast uncertainties in the interpretation of the future experiment. The prior information could possibly be correlated among model parameters or the observation noise. With no prior information this approach reduces to the fast recursive implementation of the D-optimality criterion. Synthetic geophysical tomography examples are used to illustrate the benefits of this approach.
MicroRNA‐328 is associated with (non‐small) cell lung cancer (NSCLC) brain metastasis and mediates NSCLC migration (2011)

Brain metastasis (BM) can affect ∼ 25% of nonsmall cell lung cancer (NSCLC) patients during their lifetime. Efforts to characterize patients that will develop BM have been disappointing. microRNAs (miRNAs) regulate the expression of target mRNAs. miRNAs play a role in regulating a variety of targets and, consequently, multiple pathways, which make them a powerful tool for early detection of disease, risk assessment, and prognosis. We investigated miRNAs that may serve as biomarkers to differentiate between NSCLC patients with and without BM. miRNA microarray profiling was performed on samples from clinically matched NSCLC from seven patients with BM (BM+) and six without BM (BM-). Using t-test and further qRT-PCR validation, eight miRNAs were confirmed to be significantly differentially expressed. Of these, expression of miR-328 and miR-330-3p were able to correctly classify BM+ vs. BM- patients. This classifier was used on a validation cohort (n = 15), and it correctly classified 12/15 patients. Gene expression analysis comparing A549 parental and A549 cells stably transfected to over-express miR-328 (A549-328) identified several significantly differentially expressed genes. PRKCA was one of the genes over-expressed in A549-328 cells. Additionally, A549-328 cells had significantly increased cell migration compared to A549 cells, which was significantly reduced upon PRKCA knockdown. In summary, miR-328 has a role in conferring migratory potential to NSCLC cells working in part through PRKCA and with further corroboration in additional independent cohorts, these miRNAs may be incorporated into clinical treatment decision making to stratify NSCLC patients at higher risk for developing BM.
Dynamic Power Optimization of Active Filters With Application to Zero-IF Receivers (2006)

This paper describes the use of dynamic impedance scaling and dynamic biasing to dynamically optimize the power dissipation of filters depending on the inband signal level. Transients due to the dynamical changes inside the filter are prevented thanks to the implementation of appropriate switching algorithms. To illustrate the concept, a fifth-order Chebychev low-pass active-RC filter with 100-kHz cutoff frequency, designed for partial channel selection in a zero-IF GSM receiver, is presented. The filter is fabricated in a standard 0.18-/spl mu/m CMOS process and uses a 1.2-V power supply. It consumes 2.22-mA supply current during the presence of the minimum receivable signal. The power dissipation decreases by a factor of 2 as the desired signal strength increases by 3 dB, until it reaches its minimum possible value of 0.46 mW. There are a total of five power settings in the filter.