gebhart/patrepeval huggingface dataset:
Streptogramin- and tetracycline-responsive dual regulated expression of p27Kip1 sense and antisense enables positive and negative growth control of Chinese hamster ovary cells (2001)

We constructed a dual regulated expression vector cassette (pDuoRex) whereby two heterologous genes can be independently regulated via streptogramin- and tetracycline-responsive promoters. Two different constructs containing growth-promoting and growth-inhibiting genes were stably transfected in recombinant Chinese hamster ovary (CHO) cells that express the streptogramin- and tetracycline-dependent transactivators in a dicistronic configuration. An optimally balanced heterologous growth control scenario was achieved by reciprocal expression of the growth-inhibiting human cyclin-dependent kinase inhibitor p27(Kip1) in sense (p27(Kip1)S) and antisense (p27(Kip1)AS) orientation. Exclusive expression of p27(Kip1)S resulted in complete G(1)-phase-specific growth arrest, while expression of only p27(Kip1)AS showed significantly increased proliferation compared to control cultures (both antibiotics present), presumably by decreasing host cell p27(Kip1) expression. In a second system, a derivative of pDuoRex encoding streptogramin-responsive expression of the growth-promoting SV40 small T antigen (sT) and tetracycline-regulated expression of p27(Kip1) was stably transfected into CHO cells. Expression of sT alone resulted in an increase in cell proliferation, but the expression of p27(Kip1) failed to provide the expected G(1)-specific growth arrest despite having demonstrated expression of the protein. This illustrates the difficulty in balancing the complex pathways underlying cell proliferation control through the expression of two functionally distinct genes involved in those pathways, and how a single-gene sense/antisense approach using pDuoRex can overcome this barrier to complete metabolic engineering control.
Sensitivity and frequency limitations of biquadratic active filters (1975)

A comparative study of single-amplifier filters, fifteen different two-amplifier circuits, and the state-variable three-amplifier configuration is given. The figures-of-merit used are the passive sensitivities, the <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">\omega_0</tex> gain-sensitivity products, the <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">Q</tex> gain-sensitivity products, and the <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">\omega_0</tex> and <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">Q</tex> deviations due to the amplifiers' limited bandwidth. In addition, practical matters such as input impedance and component spread are considered. Among the circuits considered a particular twoamplifier resonator emerges as the "best" biquadratic section. Some of the interesting results include a unified expression, applicable to a large class of low-sensitivity circuits, for the <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">\omega_0</tex> deviation due to the amplifier's limited frequency response. In this expression, which applies for all active filters which have low <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">\omega_0</tex> active sensitivities, the only configurationdependent quantities are the <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">Q</tex> gain-sensitivity products.
Intrinsic Charge Carrier Mobility in Single-Layer Black Phosphorus (2016)

We present a theory for single- and two-phonon charge carrier scattering in anisotropic two-dimensional semiconductors applied to single-layer black phosphorus (BP). We show that in contrast to graphene, where two-phonon processes due to the scattering by flexural phonons dominate at any practically relevant temperatures and are independent of the carrier concentration n, two-phonon scattering in BP is less important and can be considered negligible at n≳10^{13} cm^{-2}. At smaller n, however, phonons enter in the essentially anharmonic regime. Compared to the hole mobility, which does not exhibit strong anisotropy between the principal directions of BP (μ_{xx}/μ_{yy}∼1.4 at n=10^{13} cm^{-2} and T=300 K), the electron mobility is found to be significantly more anisotropic (μ_{xx}/μ_{yy}∼6.2). Absolute values of μ_{xx} do not exceed 250 (700) cm^{2} V^{-1} s^{-1} for holes (electrons), which can be considered as an upper limit for the mobility in BP at room temperature.
Vanishing points detection and line grouping for complex building facade identification (2010)

We describe a new method for automatically detecting vanishing points and group lines associated with building\nfacades from a single uncalibrated image. Accurate vanishing points detection for buildings is of importance for\nbuilding façade rectification and 3D scene reconstruction. The challenges arise from confusing scene clutter,\nocclusions, unusual building shapes and non-Manhattan street layout. Buildings usually possess many straightline\nfeatures, e.g., window and door openings, as well as their overall outline. We exploited these features and\npropose a robust line grouping technique. The method was evaluated on images from the Zubud-Zurich building\ndatabase. The experiment shows the proposed method works well with varying building structures and image\nconditions, as well as filtering out “non-building” vanishing points (e.g. vanishing directions detected from road\nboundaries).
Wafer level packaging of MEMS (2008)

Wafer level packaging plays many important roles for MEMS (micro electro mechanical systems), including cost, yield and reliability. MEMS structures on silicon chips are encapsulated between bonded wafers or by surface micromachining, and electrical interconnections are made from the cavity. Bonding at the interface, such as glass–Si anodic bonding and metal-to-metal bonding, requires electrical interconnection through the lid vias in many cases. On the other hand, lateral electrical interconnections on the surface of the chip are used for bonding with intermediate melting materials, such as low melting point glass and solder. The cavity formed by surface micromachining is made using sacrificial etching, and the openings needed for the sacrificial etching are plugged using deposition sealing methods. Vacuum packaging methods and the structures for electrical feedthrough for the interconnection are discussed in this review.
An ultrapotent synthetic nanobody neutralizes SARS-CoV-2 by stabilizing inactive Spike (2020)

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus enters host cells via an interaction between its Spike protein and the host cell receptor angiotensin-converting enzyme 2 (ACE2). By screening a yeast surface-displayed library of synthetic nanobody sequences, we developed nanobodies that disrupt the interaction between Spike and ACE2. Cryo-electron microscopy (cryo-EM) revealed that one nanobody, Nb6, binds Spike in a fully inactive conformation with its receptor binding domains locked into their inaccessible down state, incapable of binding ACE2. Affinity maturation and structure-guided design of multivalency yielded a trivalent nanobody, mNb6-tri, with femtomolar affinity for Spike and picomolar neutralization of SARS-CoV-2 infection. mNb6-tri retains function after aerosolization, lyophilization, and heat treatment, which enables aerosol-mediated delivery of this potent neutralizer directly to the airway epithelia.
The Essential Role of Research in Community Pediatrics (2005)

Several recommendations in the American Academy of Pediatrics policy statement "The Pediatrician's Role in Community Pediatrics" underscore the essential role of research as an agent of change to promote the health and well-being of children. This article provides (1) a framework for thinking about research in community pediatrics, (2) special considerations important in conducting community-level research, (3) an example of community-level research that has significantly decreased mortality in children (prevention of sudden infant death syndrome), (4) an example of a current issue illustrating the importance of community pediatrics research (promotion of school readiness), and (5) a discussion of future directions for research. Many of the leading health problems facing the United States as outlined in Healthy People 2010 are problems that affect children or have their roots in childhood and are likely to be addressed by community-level research and interventions. It seems clear that pediatricians should be learning to participate in, advocate for, and conduct more community pediatrics research.
STATE POWER AND PERSONALITY IN BYZANTINE EMPIRE: BASIC FEATURES OF THE STATUS AND LEGAL RELATIONS (2020)

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A crawling robot driven by multi-stable origami (2017)

Using origami folding to construct and actuate mechanisms and machines offers attractive opportunities from small, scalable, and cheap robots to deployable adaptive structures. This paper presents the design of a bio-inspired origami crawling robot constructed by folding sheets of paper. The origami building block structure is based on the Kresling crease pattern (CP), a chiral tower with a polygonal base, which expands and contracts through coupled longitudinal and rotational motion similar to a screw. We design the origami to have multi-stable structural equilibria which can be tuned by changing the folding CP. Kinematic analysis of these structures based on rigid-plates and hinges at fold lines precludes the shape transformation associated with the bistability of the physical models. To capture the kinematics of the bi-stable origami, the panels' deformation behavior is modeled utilizing principles of virtual folds. Virtual folds approximate material bending by hinged, rigid panels, which facilitates the development of a kinematic solution via rigid-plate rotation analysis. As such, the kinetics and stability of folded structures are investigated by assigning suitable torsional spring constants to the fold lines. The results presented demonstrate the effect of fold-pattern geometries on the snapping behavior of the bi-stable origami structure based on the Kresling pattern. The crawling robot is presented as a case study for the use of this origami structure to mimic crawling locomotion. The robot is comprised of two origami towers nested inside a paper bellow, and connected by 3D printed end plates. DC motors are used to actuate the expansion and contraction of the internal origami structures to achieve forward locomotion and steering. Beyond locomotion, this simple design can find applications in manipulators, booms, and active structures.
Color Gamut Mapping (2008)

Gamut mapping algorithms, implemented by color management systems, are an integral part of the color reproduction process. By adjusting the colors with appropriate algorithms, gamut mapping enables original colors to fit inside differently shaped color gamuts and authentically transfers images across a range of media. This book illustrates the range of possible gamut mapping strategies for cross-media color reproduction, evaluates the performance of various options and advises on designing new, improved solutions. Starting with overviews of color science, reproduction and management, the text includes: a detailed survey of 90+ gamut mapping algorithms covering color-by-color reduction and expansion, spatial reduction, spectral reduction and gamut mapping for niche applications; a step-by-step example of a colors journey from original to reproduction, via a digital workflow; a detailed analysis of color gamut computation, including a comparison of alternative techniques and an illustration of the gamuts of salient color sets and media; a presentation of both measurement-based and psychovisual evaluation of individual color reproductions; an overview of alternative approaches to gamut mapping proposed by the ISO and the CIE including an analysis of the building blocks of gamut mapping algorithms and the factors affecting their performance. Color Gamut Mapping is a comprehensive resource for practicing color and imaging engineers, scientists and researchers working in the development of imaging devices, software and solutions. It is also a valuable reference for students of color and imaging science, as well as photographers, graphic designers and artists.
Fuzzy c-Means Algorithms for Very Large Data (2012)

Very large (VL) data or big data are any data that you cannot load into your computer's working memory. This is not an objective definition, but a definition that is easy to understand and one that is practical, because there is a dataset too big for any computer you might use; hence, this is VL data for you. Clustering is one of the primary tasks used in the pattern recognition and data mining communities to search VL databases (including VL images) in various applications, and so, clustering algorithms that scale well to VL data are important and useful. This paper compares the efficacy of three different implementations of techniques aimed to extend fuzzy c-means (FCM) clustering to VL data. Specifically, we compare methods that are based on 1) sampling followed by noniterative extension; 2) incremental techniques that make one sequential pass through subsets of the data; and 3) kernelized versions of FCM that provide approximations based on sampling, including three proposed algorithms. We use both loadable and VL datasets to conduct the numerical experiments that facilitate comparisons based on time and space complexity, speed, quality of approximations to batch FCM (for loadable data), and assessment of matches between partitions and ground truth. Empirical results show that random sampling plus extension FCM, bit-reduced FCM, and approximate kernel FCM are good choices to approximate FCM for VL data. We conclude by demonstrating the VL algorithms on a dataset with 5 billion objects and presenting a set of recommendations regarding the use of different VL FCM clustering schemes.
Direct contact heat transfer – a better way to high efficiency and compactness (1984)

Abstract A new concept in direct contact heat transfer is proposed which consists of carrying out combustion, and maintaining the resulting flame, directly within a flowing stream of water. The concept has been experimentally demonstrated in a prototype designed to handle 1200 kg/h of water. The system comes to steady state within 30 seconds. The process yields thermal efficiencies up to 97.5% and heat transfer coefficients up to 14 times those found in industrial spray tower contactors. The novel process possesses a great potential for industrial applications, such as process hot water supply, enhanced oil recovery using hot water, and compact steam generators.
Computer Vision Annotation Tool (2019)

CVAT is free, online, interactive video and image annotation tool for computer vision. It is being used by our team to annotate million of objects with different properties. Many UI and UX decisions are based on feedbacks from professional data annotation team.
Electronic states in magnetic nanostructures (1998)

This paper provides a survey of electronic states in magnetic nanostructures, how they differ from bulk states, and how these changes are related to interesting magnetic phenomena such as oscillatory coupling and giant magnetoresistance (GMR). After explaining the role of quantum well states and spin-dependent electron reflectivity in magnetic multilayers, we turn our attention to lower-dimensional structures such as stripes and dots. Fabrication methods are described that use a stepped surface as template. For monitoring the growth mode, it is critical to distinguish and identify different metals at a surface by scanning tunneling microscopy (STM). This is achieved via resonant tunneling through metal-specific surface and image states.
Synthesis of New Crown Ethers Containing Appended Pyridine, 10-hydroxybenzoquinoline, 8-hydroxyquinoline and 2-amino-1-hydroxybiphenyl Sidearms (2002)

Twelve crown ethers containing one or two arms were synthesized. Two methods were used to attach arms to the azacrown ethers. Ligands 4 - 12 were prepared by a nucleophilic substitution of secondary macrocyclic amine functions on RX ( X=bromide or tosylate groups). Ligands 13 - 15 were obtained via a Mannich reaction of secondary macrocyclic amines with 5-chloro-8-hydroxyquinoline or a substituted-phenol. Diaza-18-crown-6 was treated with 2-bromomethyl-9-methyl-1,10-phenanthroline at the same conditions in which 4 - 8 and 10 - 12 were prepared. In this case, the main product was the diazacrown ether containing one arm. Twelve new aza-crown ethers bearing aromatic and aliphatic side arms were prepared by nucleophilic substitution by secondary macrocyclic amine functions on halide or tosylates or via a Mannich reaction of the macrocyclic secondary amines with phenolic compounds. R=derivatives of pyridine, 8-hydroxyquiniline, and 1-hydroxybiphenyl. Crown ether include aza-15-crown-5 diaza-18-crown-6 diazatrithia-15 (and 16)-crown-6 and diaza-21-crown-7
HPLC/circular dichroism detection using a split‐type flow cell for polarized photometric detector (1997)

HPLC/circular dichroism detection using a conventional spectrophotometric detector has been established. A split-type flow cell developed for a polarized photometric detector, which is a non-modulated polarimeter, was inserted into this detector. When two rays of circularly polarized light with opposite rotational senses to each other were incident in both cells by combining a polarizer and a 1/4 wave retarder in front of the cell assembly, the output from this spectrophotometric detector became solely the signal based on circular dichroism. Selective detection of a chiral analyte, in which the ratio of the difference in the absorbance between two rays mentioned above and the absorbance by normal rays is over 1/1000, is feasible using the proposed instrument. © 1997 John Wiley & Sons, Ltd.
Relationship of C5a receptor modulation to the functional responsiveness of human polymorphonuclear leukocytes to C5a (1993)

The relationship of C5a receptor expression on human polymorphonuclear leukocytes (PMN) to the functional response of these cells to C5a was studied using flow cytometry. C5a receptor expression was determined with a fluorescein conjugate of C5a and oxidative burst activity was monitored by conversion of dichlorofluorescein to dichlorofluorescein (DCF) as a measure of H2O2 production. These studies showed that after incubation of PMN with increasing concentrations of C5a, and allowing for internalization of bound ligand, more than 40% of the cell surface C5a receptors were internalized before the DCF response to optimal concentrations of C5a was decreased below the levels for untreated control cells. Although C5a responsiveness was lost after preincubation with 10(-8) M C5a, cells remained responsive to formyl peptide. In other studies, cells were preincubated with unlabeled C5a under conditions that provided for internalization of nearly all C5a receptors. PMN were then cultured for up to 90 min and monitored for C5a receptor reexpression and return of cell function. In these studies, the DCF response of PMN to C5a returned to 100% much earlier than the cells regained full expression of C5a receptors. The DCF response to formyl peptide remained intact throughout the period of C5a receptor reexpression. These studies showed that once > 40% of the original population of C5a receptors are reexpressed on the PMN, that these cells regain 100% of their functional responsiveness to C5a in the DCF assay. Evaluation of the affinity and number of C5a receptors using 125I-labeled C5a after receptor reexpression showed that maximal receptor reexpression was approximately 73% of that obtained with control cells and the Kd of reexpressed receptors was 0.60 vs 0.94 nM for control cells. These studies demonstrate that only a portion of the total C5a receptors expressed on PMN are essential to stimulate a 100% functional response in PMN and that the reexpressed receptors are capable of transducing a signal that activates the oxidative burst in these cells.
The GlobeFish and the GlobeMouse (2006)

We introduce two new six degree of freedom desktop input devices based on the key concept of combining forceless isotonic rotational input with force-requiring elastic translational input. The GlobeFish consists of a custom three degrees of freedom trackball which is elastically connected to a frame. The trackball is accessible from the top and bottom and can be moved slightly in all spatial directions by using force. The GlobeMouse device works in a similar way. Here the trackball is placed on top of a movable base, which requires to change the grip on the device to switch between rotating the trackball and moving the base.Our devices are manipulated with the fingertips allowing precise interaction with virtual objects. The elastic translation allows uniform input for all three axes and the isotonic trackball provides a natural mapping for rotations. Our user study revealed that the new devices perform significantly better in a docking task in comparison to the SpaceMouse, an integrated six degrees of freedom controller. Subjective data confirmed these results.
Mobile Handsets as Sensing Nodes in an Auto-Configured Hierarchical Cognitive Radio Network Scheme for Immediate Post-Disaster Communications (2009)

A critical problem after a natural/manmade disaster is to provide immediate post-disaster communication links between the disaster victims and some overlay networks. This paper proposes a novel scheme that uses the surviving Mobile handSets (MS) as sensing nodes to form an auto-configured Hierarchical Cognitive Radio Network (H-CRN). The implementation of this H-CRN is explained through detailed problem scenario statement and step-by-step implementation of automatic identification of emergency situation by the MS nodes. An overview of the cross-layer framework used by the MS nodes is also presented. This novel scheme is tested through some hypothesis along with probability calculations for successful identification of emergency situation, formation of ad hoc group and Emergency Beacon Message (EBM) transmission.
Empirical Correlations between Krafft Temperature and Tail Length for Amidosulfobetaine Surfactants in the Presence of Inorganic Salt (2011)

Long-chain amidosulfobetaine surfactants, 3-(N-fattyamidopropyl-N,N-dimethyl ammonium) propanesulfonates (n-DAS, n > 18), are insoluble in pure water due to their high Krafft temperature (T(K)), while they are soluble when inorganic salt is added to the surfactant solution as the T(K) of these zwitterionic surfactants is decreased. The influence of the salt content and ionic species of the added electrolytes on the T(K) of the series of amidosulfobetaine surfactants was examined by means of UV-vis spectrophometry and visual inspection. It was found that the T(K) of these surfactants depends strongly on not only the hydrophobic alkyl length (n), but also the salinity of the aqueous environment. When the salt concentration is increased from 0 to 100 mM, the T(K) shows a sharp decrease; when the salinity is fixed between 100 and 2000 mM, the T(K) varies linearly with n with a slope of ~7.7 irrespective of the salt species and the salt content. When the salt concentration is further increased above 2000 mM, a linear function is still observed, but the slope increases slightly.