gebhart/patrepeval huggingface dataset:
Valproic acid assisted reprogramming of fibroblasts for generation of pluripotent stem cells in buffalo (Bubalus bubalis) (2016)

Generation of pluripotent stem cells by reprogramming somatic cells of quality animals has numerous potential applications in agricultural and biomedical sciences. Unfortunately, till now, reprogramming of buffalo fetal fibroblast cells (bFFs) has been very ineffient despite intensive efforts. Here, we attempted to enhance reprogramming efficiency by using the HDAC inhibitor valproic acid (VPA) in bFFs transfected with pLentG-KOSM pseudo virus carrying mouse specific pluripotent genes. FACS analysis revealed that VPA treatment significantly increased (p < 0.05) GFP<sup>+</sup> cells in comparison to VPA untreated control. Further, among different concentrations, 1.5 mM VPA was found to be optimal, increasing about 5 fold GFP<sup>+</sup> cells and 2.5-fold GFP+ colonies with significantly (P < 0.05) larger size as compared to control. These colonies were further propagated and characterised. The colonies displayed embryonic stem cell (ESC)-like morphology, normal karyotype, and were positive for alkaline phosphatase staining as well as immune-positive for the ESC specific markers Oct4, Nanog, SSEA1, TRA-1-60 and TRA-1-81. The primary colonies revealed significantly higher (P < 0.05) expression of pluripotent genes than control, which declined gradually on subsequent passages. The reprogrammed cells readily formed embryoid bodies in vitro and cells of all three germ layers. These results indicated that VPA treatment of viral transducted cells can improve the generation of induced pluripotent stem cells and help their long term maintenance in buffalo.
The Zinc-Finger Protein Zat12 Plays a Central Role in Reactive Oxygen and Abiotic Stress Signaling in Arabidopsis  (2005)

Plant acclimation to environmental stress is controlled by a complex network of regulatory genes that compose distinct stress-response regulons. In contrast to many signaling and regulatory genes that are stress specific, the zinc-finger protein Zat12 responds to a large number of biotic and abiotic stresses. Zat12 is thought to be involved in cold and oxidative stress signaling in Arabidopsis (Arabidopsis thaliana); however, its mode of action and regulation are largely unknown. Using a fusion between the Zat12 promoter and the reporter gene luciferase, we demonstrate that Zat12 expression is activated at the transcriptional level during different abiotic stresses and in response to a wound-induced systemic signal. Using Zat12 gain- and loss-of-function lines, we assign a function for Zat12 during oxidative, osmotic, salinity, high light, and heat stresses. Transcriptional profiling of Zat12-overexpressing plants and wild-type plants subjected to H(2)O(2) stress revealed that constitutive expression of Zat12 in Arabidopsis results in the enhanced expression of oxidative- and light stress-response transcripts. Under specific growth conditions, Zat12 may therefore regulate a collection of transcripts involved in the response of Arabidopsis to high light and oxidative stress. Our results suggest that Zat12 plays a central role in reactive oxygen and abiotic stress signaling in Arabidopsis.
A polyelectrolyte bearing metal ion receptors and electrostatic functionality for layer‐by‐layer self‐assembly (2004)

Abstract A polyelectrolyte (BiPE) containing bipyridine ligands as metal ion receptors and quaternary ammonium groups is described, which can be assembled via electrostatic interactions or metal ion coordination. Electrostatic layer‐by‐layer self‐assembly of BiPE with sodium poly(styrene sulfonate) (PSS) as oppositely charged component results in striated multilayers. The BiPE/PSS multilayers can reversibly bind and release transition metal ions including Fe(II), Ni(II), and Zn(II). Formation of 2‐D arrays of metallo‐units is achieved by μ‐contact stamping transition metal salts onto the BiPE/PSS interface. Also, multilayers of BiPE are readily assembled through metal ion coordination. Due to the reversible nature of metal ion coordination, exposure of the multilayers to EDTA causes instant disassembly of the layer, a property needed to implement stimulus triggered release functions. The importance of metal ion coordination for multilayer formation is demonstrated by force‐distance curves measured with AFM.
Optimal quantum control for conditional rotation of exciton qubits in semiconductor quantum dots (2011)

Pulse-shaping protocols for subpicosecond optically controlled quantum gates in semiconductor quantum dots are reported. Our emphasis is the development of shaping schemes for either amplitude or phase control of the pulse that are easily implemented using commercial pulse shapers and femtosecond laser systems. We illustrate the efficacy of our approach through simulations of a controlled-rotation gate in a realistic In(Ga)As quantum dot with electronic structure calculated using eight-band, strain-dependent $\mathbit{k}\ifmmode\cdot\else\textperiodcentered\fi{}\mathbit{p}$ theory. Our results show that amplitude- and phase-shaping protocols both lead to substantial improvements in fidelity when compared with transform-limited pulses with equivalent gate times. Dephasing was found to have a minimal effect on the gate fidelities due to the ultrafast time scale of the quantum operations.
Pattern Matching in LZW Compressed Files (2005)

Compressed pattern matching is an emerging research area that addresses the following problem: Given a text file in compressed format and a pattern, report the occurrence(s) of the pattern in the file with minimal (or no) decompression. In this paper, we report our work on compressed pattern matching in LZW compressed files. The work includes an extension of Amir et al.'s well-known "almost-optimal" algorithm. The original Amir et al.'s algorithm has been improved to search not only the first occurrence of the pattern but also all other occurrences. A faster implementation for so-called "simple patterns" is also proposed. The work also includes a novel multiple-pattern matching algorithm using the Aho-Corasick algorithm. The algorithm takes O(mt+n+r) time with O(mt) extra space, where n is the size of the compressed file, m is the total length of all patterns, t is the size of the LZW trie, and r is the number of occurrences of the patterns. Extensive experiments have been conducted to test the performance of our algorithms and to compare with other well-known compressed pattern matching algorithms, particularly the BWT-based algorithms and another similar multiple-pattern matching algorithm by Kida et al. that also uses the Aho-Corasick algorithm on the LZW compressed data. The results showed that our multiple-pattern matching algorithm is competitive among the best compressed pattern-matching algorithms and is practically the fastest among all approaches when the number of patterns is not very large. Therefore, our algorithm is preferable for general string matching applications. The proposed algorithm is efficient for large files and it is particularly efficient when being applied on archive search if the archives are compressed with a common LZW trie. LZW is one of the most efficient and popular compression algorithms used extensively and our method requires no modification on the compression algorithm. The work reported in this paper, therefore, has great economic and market potential.
Calcium regulation of actin filament capping and monomer binding by macrophage capping protein. (1994)

Macrophage capping protein (MCP) is a unique member of the gelsolin-villin family of calcium-activated barbed end capping proteins which in micromolar Ca2+ also binds actin monomers and nucleates actin assembly. Unlike gelsolin, MCP cannot sever actin filaments, and its Ca(2+)-dependent interaction with actin is completely reversible. The Ca2+ binding properties of MCP and its Ca(2+)-dependent functions were studied quantitatively. MCP undergoes a Ca(2+)-induced conformational change as evidenced by different chymotryptic digest patterns in 0.2 mM CaCl2 compared with 2 mM EGTA. MCP has a single low affinity Ca2+ binding site (KD = 37 microM). Binding of MCP to actin monomers requires a similar Ca2+ concentration ([Ca2+]0.5 = 62 microM) suggesting that MCP.Ca2+ complex formation promotes monomer binding. In contrast, filament capping by MCP requires 1/60th of the Ca2+ concentration required for monomer binding, half-maximal capping occurring at 1 microM Ca2+. The marked difference in the Ca2+ sensitivity of these two functions indicate that MCP's primary actin regulatory role in the macrophage is likely to be capping of the barbed ends of actin filaments.
Ovulation rate, follicle population and FSH levels in cyclic rats after administration of an inhibin-neutralizing antiserum (1991)

Ovulation rate, follicle growth, serum FSH and oestradiol concentrations were studied after a single intraperitoneal injection of inhibin antiserum in 5-day-cyclic rats. Control rats received (non-immune) serum from castrated sheep or saline. Rats were injected at 10.00 h on dioestrus-1 (D1), i.e. the day following the day of oestrus, or at 17.00 h on dioestrus-2 (D2). The ovaries were excised at necropsy 48 h after injection, or at first or second oestrus after injection. After routine histology fresh corpora lutea were counted and/or differential follicle counts were made. Results from rats injected with either (non-immune) serum from castrated sheep or with saline were not different and were therefore combined to form the control group. The activity of inhibin-neutralizing antibodies in the circulation of antiserum-treated rats was reduced by approximately 39% between 8 h and second oestrus after injection, as determined by the binding of purified bioactive radioiodinated 31 kDa bovine inhibin. Rats were injected on D1 and killed at first oestrus. The number of fresh corpora lutea was significantly higher in antiserum-treated rats than in controls (13.9 +/- 0.4 vs 11.8 +/- 0.4; P less than 0.05). Other rats injected on D1 were killed either 48 h or at the second oestrus after injection. Blood was collected 8, 16, 24 and 48 h and at first and second oestrus after injection.(ABSTRACT TRUNCATED AT 250 WORDS)
Correlation between the macroscopic ferroelectric material properties of Si:HfO <sub>2</sub> and the statistics of 28 nm FeFET memory arrays (2016)

With the discovery of ferroelectric hafnium oxide (FE-HfO2), the ferroelectric field effect transistor (FeFET), a long-term contender for non-volatile data storage, has finally managed to scale to the 2× nm technology node. Here for the first time, we correlate the thickness dependent ferroelectric properties of Si:HfO2 with the memory characteristics of small (56 bit) FeFET arrays. First, an electrical and structural analysis of metal-ferroelectric-metal capacitors is given. Even though possessing room-temperature deposited top electrodes, TiN / Si:HfO2 (20 nm) / TiN capacitors are showing deteriorated polarization characteristics as compared to their 10 nm Si:HfO2 counterparts. This could be attributed to an increased monoclinic phase fraction, as indicated by small-signal capacitance voltage and grazing incidence X-ray diffraction measurements. Identical Si:HfO2 thin films with thicknesses of 10 nm and 20 nm respectively, were utilized in a 28 nm high-k metal-gate CMOS flow to form small FeFET memory arrays of AND architecture. After extracting the most suitable operating conditions from erase matrix, single cell evaluation was performed by standard VP/3 program and a novel VP/3 positive-source drain erase scheme. Array cells incorporating 10 nm Si:HfO2 films showed a maximum memory window of 1.03 V whereas cells incorporating 20 nm Si:HfO2 films could reach up to 1.57 V. Moreover, in accordance to the basic material properties, the previously observed increased monoclinic phase fraction in 20 nm Si:HfO2 thin films correlate well with a reduced number of functional FeFET cells.
Hot Carrier Electroluminescence from a Single Carbon Nanotube (2004)

We measure the spectrum and efficiency of the infrared light emission from ambipolar carbon nanotube field-effect transistors. The width of the emission peak is strongly device-structure dependent. Long devices (∼50 μm) show narrow spectral peaks that we attribute to relaxed carrier recombination, while short devices (∼500 nm) show broad peaks due to hot carrier recombination. The hot carrier distribution is limited to energies below the energies of the optical/zone boundary phonons near 180 meV. The efficiency of the radiative recombination is between 10-6 and 10-7 photons/electron−hole pair, and the possible quenching mechanisms are discussed.
Cross-domain sparse coding (2013)

Sparse coding has shown its power as an effective data representation method. However, up to now, all the sparse coding approaches are limited within the single domain learning problem. In this paper, we extend the sparse coding to cross domain learning problem, which tries to learn from a source domain to a target domain with significant different distribution. We impose the Maximum Mean Discrepancy (MMD) criterion to reduce the cross-domain distribution difference of sparse codes, and also regularize the sparse codes by the class labels of the samples from both domains to increase the discriminative ability. The encouraging experiment results of the proposed cross-domain sparse coding algorithm on two challenging tasks --- image classification of photograph and oil painting domains, and multiple user spam detection --- show the advantage of the proposed method over other cross-domain data representation methods.
Optlang: An algebraic modeling language for mathematical optimization (2017)

Optlang is a Python package implementing a modeling language for solving mathematical optimization problems, i.e., maximizing or minimizing an objective function over a set of variables subject to a number of constraints. It provides a common native Python interface to a series of optimization tools, so different solver backends can be used and changed in a transparent way. Optlang’s object-oriented API takes advantage of the symbolic math library SymPy (Team 2016) to allow objective functions and constraints to be easily formulated algebraically from symbolic expressions of variables. Optlang targets scientists who can thus focus on formulating optimization problems based on mathematical equations derived from domain knowledge. Solver interfaces can be added by subclassing the four main classes of the optlang API (Variable, Constraint, Objective, and Model) and implementing the relevant API functions.
Optical and Scintillation Properties of Inorganic Scintillators in High Energy Physics (2008)

This paper presents a comparative study of optical and scintillation properties for various inorganic crystal scintillators, which are used, or actively pursued, by the high energy physics community for experiments. Transmittance, excitation and photo-luminescence spectra were measured for samples with a dimension of 1.5 radiation length. The transmittance data are compared to the theoretical limit calculated by using refractive index, assuming no internal absorption. Refractive index of lutetium oxyorthosilicate and lutetium-yttrium oxyorthosilicate was measured by using a V-prism. Light output was measured for these samples with Tyvek paper wrapping, and the result is presented with the quantum efficiency of the readout devices taken out. Temperature coefficient of the light output was also measured. The result of these measurements will be used in the summary table of the inorganic scintillator section for the 2008 edition of the particle data book.
Complex and dynamic redistribution of NF-κB signaling intermediates in response to T cell receptor stimulation (2004)

The central zone of the supramolecular activation cluster (c-SMAC) is a zone of T cell receptor (TCR) enrichment that forms at a T cell/antigen-presenting cell (APC) junction in response to antigen stimulation. We demonstrate that there is a surprisingly complex relocalization process that brings PKC and Bcl10, two intermediates in TCR activation of NF-kappaB, to the cytoplasmic face of the c-SMAC. TCR activation causes enrichment of PKC at the c-SMAC, followed by Bcl10 relocalization to punctate cytoplasmic structures, often at sites distant from the c-SMAC. These Bcl10 structures then undergo further relocalization, becoming enriched at the c-SMAC. TCR activation of NF-kappaB therefore involves the dynamic relocalization of multiple signaling intermediates, with distinct phases proximal to and distant from the c-SMAC.
10 W high-efficiency high-brightness tapered diode lasers at 976 nm (2008)

Tapered diode lasers combine high output power and a beam quality near to the diffraction limit resulting in very high brightness. Therefore, they are finding use in a wide range of applications today, such as pumping of rare-earth-doped fibre amplifiers, tunable frequency doubling of diode lasers for blue-green outputs, and non linear spectroscopy. Due to increasing brightness and lifetime tapered lasers even become attractive for material processing and for telecom applications like pumping of Er-doped fiber amplifiers or raman amplifiers. In order to further enhance the brightness of tapered diode lasers the output power has to be increased while simultaneously the beam quality has to be kept near the diffraction limit. For this purpose we have grown low modal gain, single quantum well InGaAs/AlGaAs devices emitting at 976 nm by molecular beam epitaxy. The lateral design of the investigated laser diodes consists of a tapered section and a ridge-waveguide section. Since it has been shown by previous simulations and experiments that longer tapered sections allow higher output power with unchanged beam quality, we use tapered section lengths of 2000 μm, 3000 μm and 4000 μm. The beam quality parameter M<sup>2</sup> and output powers as well as the nearfields of the different structures were carefully investigated. For longer devices we reach an optical output power of more than 10 W per single emitter in continuous wave mode (cw) without any distinct thermal rollover.
An All-Plastic and Flexible Electrochromic Device Based on Elastomeric Blends (1998)

All-plastic and flexible electrochromic devices will facilitate new technological applications, such as information display and storage, in the automotive industry (rear-view mirrors) and in architecture (smart windows). Here the authors describe an all-plastic and flexible solid-state electrochromic device using two optically complementary conductive polymer blends deposited on ITO-PET (indium tin oxide–poly(ethyleneterephthalate)), and a polymeric electrolyte. The device was constructed under atmospheric conditions and showed good optical characteristics.
Algemene voorwaarden en het Europese gelijkheidsbeginsel : De verhouding tussen de artikelen 6:235 lid 1 en 6:247 lid 2 BW bezien in het licht van artikel 18 VWEU (2018)

De regels voor grote ondernemingen en internationale contracten in de algemenevoorwaardenregeling van afdeling 6.5.3 BW zijn al lang onderwerp van kritiek. In deze bijdrage wordt de verhouding tussen de desbetreffende wetsartikelen geëvalueerd op basis van het Europese gelijkheidsbeginsel, dat discriminatie naar nationaliteit verbiedt. Dit leidt tot de – wellicht verrassende – conclusie dat niet artikel 6:247 lid 2 maar artikel 6:235 lid 1 BW de meeste problemen oplevert vanuit nationaal en Europees perspectief.
Design of diplexers with prescribed crossover loss using singly terminated filters (2002)

Two new methods are described to design frequency symmetric diplexers with any realizable prescribed crossover attenuation. The two methods are based on the use of singly terminated low-pass (LP) and high-pass (HP) filter pairs that are readily available or that can be easily synthesized. In these methods, the pass-band corner frequencies of LP and HP filters are scaled to give the desired crossover attenuation. The approach can use filters with maximally flat or equiripple pass-bands and general stopbands, thus enabling one to design diplexers with or without transmission zeros easily. Some examples are designed and realized in suspended stripline structure.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Permeability of Mouse Oocytes and Embryos at Various Developmental Stages to Five Cryoprotectants (2005)

To assess the permeability of mouse oocytes and embryos, matured oocytes and embryos at various stages of development were placed in five cryoprotectant solutions at 25 C for 25 min. From the cross-sectional areas of the oocytes/embryos, the relative change in volume was analyzed. In oocytes, shrinkage was least extensive and recovery was quickest in the propylene glycol solution, showing that propylene glycol permeates the oocytes most rapidly. Dimethyl sulfoxide, acetamide, and ethylene glycol permeated the oocytes slightly more slowly than propylene glycol. The oocytes in glycerol shrunk extensively and then expanded marginally, indicating slow permeation. The volume changes of 1-cell and 2-cell embryos were similar to those of oocytes, showing little change in permeability. In 8-cell embryos, the volume recovered much faster than in the earlier stages especially in glycerol and acetamide. In morulae, the volume recovery was much faster in glycerol and in ethylene glycol; in ethylene glycol, the extent of shrinkage was small and the recovery was fast, indicating an extremely rapid permeation. Although the permeability of oocytes/embryos generally increased as embryo development proceeded, the degree of increase varied greatly among the cryoprotectants. Interestingly, the volume change in propylene glycol was virtually unaffected by the stage of development. Such information will be valuable for determining a suitable protocol for the cryopreservation of oocytes/embryos at different stages of development.
Synthesis and evaluation of non-catechol D-1 and D-2 dopamine receptor agonists: benzimidazol-2-one, benzoxazol-2-one, and the highly potent: benzothiazol-2-one 7-ethylamines (1987)

Our interest in identifying D-1 and D-2 dopamine receptor agonists that are not catechols led us to extend previous studies with oxindoles by investigating analogues of dopamine, N,N-dipropyldopamine, m-tyramine, N,N-dipropyl-m-tyramine, and epinine in which the m-hydroxyl is replaced by the NH portion of a thiazol-2-one, oxazol-2-one, or imidazol-2-one group fused to the 2,3-position. These compounds were evaluated for their affinity and agonist activity at D-1 and D-2 receptors by using in vitro assays. Replacement of the m-hydroxy in N,N-dipropyldopamine with the thiazol-2-one group resulted in a dramatic increase in D-2 receptor affinity and activity compared to that of N,N-dipropyldopamine itself or that of the corresponding oxindole, 1. The resulting compound, 7-hydroxy-4-[2-(di-n-propylamino)ethyl]benzothiazol-2(3H)-one (4), is the most potent D-2 receptor agonist reported to date in the field-stimulated rabbit ear artery (ED50 = 0.028 nM). The benzoxazol-2-one (6), benzimidazol-2-one (5), and isatin (51) analogues showed D-2 receptor agonist potency similar to that of 1. The des-7-hydroxyl analogue of 4 (21) also has enhanced D-2 receptor activity compared to that of the corresponding oxindole, 8. 7-Hydroxy-4-(2-aminoethyl)benzothiazol-2(3H)-one, 27, a non-catechol, has enhanced D-1 and D-2 receptor activity in vitro compared to that of the corresponding oxindole, 7. In vivo, 27 increased renal blood flow and decreased blood pressure in the dog. However, these effects were mediated primarily by D-2 receptor agonist activity. This may be a result of the D-1 partial agonist activity of 27 coupled with its potent D-2 receptor activity.