gebhart/patrepeval huggingface dataset:
Mechanisms of Botulinum Toxin Type A Action on Pain (2019)

Already a well-established treatment for different autonomic and movement disorders, the use of botulinum toxin type A (BoNT/A) in pain conditions is now continuously expanding. Currently, the only approved use of BoNT/A in relation to pain is the treatment of chronic migraines. However, controlled clinical studies show promising results in neuropathic and other chronic pain disorders. In comparison with other conventional and non-conventional analgesic drugs, the greatest advantages of BoNT/A use are its sustained effect after a single application and its safety. Its efficacy in certain therapy-resistant pain conditions is of special importance. Novel results in recent years has led to a better understanding of its actions, although further experimental and clinical research is warranted. Here, we summarize the effects contributing to these advantageous properties of BoNT/A in pain therapy, specific actions along the nociceptive pathway, consequences of its central activities, the molecular mechanisms of actions in neurons, and general pharmacokinetic parameters.
HIV-1 Vif inhibits G to A hypermutations catalyzed by virus-encapsidated APOBEC3G to maintain HIV-1 infectivity (2014)

HIV-1 viral infectivity factor (Vif) is an essential accessory protein for HIV-1 replication. The predominant function of Vif is to counteract Apolipoprotein B mRNA-editing enzyme-catalytic polypeptide-like 3G (APOBEC3G, A3G), a potent host restriction factor that inhibits HIV-1 replication. Vif mediates the proteasomal degradation of A3G and inhibits A3G translation, thus diminishing the pool of A3G that is available to be packaged into budding virion. Although Vif is robust in degrading A3G, the protection provided against A3G is not absolute. Clinical and laboratory evidence have shown that A3G is not completely excluded from HIV-1 viral particles during HIV-1 replication. It remains unclear why the viral samples are still infectious when A3G has been packaged into the virions. In this study, we provide evidence that Vif continues to protect HIV-1 from the deleterious effects of A3G, even after packaging of A3G has occurred. When equal amounts of A3G were packaged into budding virions, the virus expressing functional Vif was more infectious and incurred fewer G to A hypermutations in the second round of infection compared to Vif-deficient virus. A Vif mutant with a defect in viral packaging showed a reduced ability to protect the HIV-1 genome from G to A hypermutations. Our data suggest that even packaged A3G is still under the tyranny of Vif. Our work brings to light an additional caveat for any therapy that hopes to exploit the Vif-A3G axis. The ideal strategy would not only enhance A3G viral packaging, but also reduce HIV-1 Vif viral encapsidation.
In different organisms, the mode of interaction between two signaling proteins is not necessarily conserved (2004)

Although interfaces mediating protein-protein interactions are thought to be under strong evolutionary constraints, binding of the chemotaxis histidine kinase CheA to its phosphorylation target CheY suggests otherwise. The structure of Thermotoga maritima CheA domain P2 in complex with CheY reveals a different association than that observed for the same Escherichia coli proteins. Similar regions of CheY bind CheA P2 in the two systems, but the CheA P2 domains differ by an approximately 90 degrees rotation. CheA binds CheY with identical affinity in T. maritima and E. coli at the vastly different temperatures where the respective organisms live. Distinct sets of P2 residues mediate CheY binding in the two complexes; conservation patterns of these residues in CheA and compensations in CheY delineate two families of prokaryotic chemotaxis systems. A protein complex that has the same components and general function in different organisms, but an altered structure, indicates unanticipated complexity in the evolution of protein-protein interactions and cautions against extrapolating structural data from homologs.
Large area efficient interface layer free monolithic perovskite/homo-junction-silicon tandem solar cell with over 20% efficiency (2018)

A simple and scalable interface-layer free monolithic perovskite/silicon tandem has been demonstrated achieving over 20% efficiency on a large area.
Genome Sequence of the Self-Flocculating Strain Saccharomyces cerevisiae SPSC01 (2018)

The self-flocculation of yeast cells presents advantages for continuous ethanol fermentation such as their self-immobilization within fermenters for high density to improve ethanol productivity and cost-effective biomass recovery by gravity sedimentation. We sequenced and analyzed the genome of the self-flocculating <i>Saccharomyces cerevisiae</i> SPSC01 for the industrial production of fuel ethanol.
Direct methods for visual scene reconstruction (2002)

There has been a lot of activity recently surrounding the reconstruction of photorealistic 3D scenes and high-resolution images from video sequences. We present some of our recent work in this area, which is based on the registration of multiple images (views) in a projective framework. Unlike most other techniques, we do not rely on special features to form a projective basis. Instead, we directly solve a least-squares estimation problem in the unknown structure and motion parameters, which leads to statistically optimal estimates. We discuss algorithms for both constructing planar and panoramic mosaics, and for projective depth recovery. We also speculate about the ultimate usefulness of projective approaches to visual scene reconstruction.
Final Report: Mobile Surveillance and Wireless Communication Systems Field Operational Test Volume 2: FOT Objectives, Organization, System Design, Results, Conclusions and Recommendations (1999)

The Mobile Surveillance and Wireless Communication Systems Field Operational Test (FOT) evaluated the performance of wireless traffic detection and communications systems in areas where permanent detectors, electrical power, and landline communications were not available. The FOT partners designed and built six surveillance and three ramp meter trailers, a video and data retransmission or relay site, and video and data reception facilities at the Caltrans District 12 and Anaheim Traffic Management Center (TMCs) and the University of California at Irvine Institute of Transportation Studies(UCI-ITS) Laboratory.&nbsp; The system was evaluated in two different types of tests.&nbsp; The Anaheim Special Event Test assessed the surveillance trailers in an application that transmitted video imagery in support of arterial traffic control during a special event. The Interstate-5 (I-5) Test examined the use of the mobile surveillance and ramp meter trailers to transmit video imagery and data in support of freeway ramp metering.
Monoclonal antibody-aided characterization of cellular p220 in uninfected and poliovirus-infected HeLa cells: subcellular distribution and identification of conformers (1987)

A monoclonal antibody directed against the Mr-220,000 subunit (p220) of the mRNA cap-binding complex has been prepared and used to analyze the sucrose gradient sedimentation and subcellular location of p220 and its poliovirus-induced cleavage products. The antibody reacted with p220 on immunoblots of cell lysates from uninfected cells, but only with several smaller polypeptides, the p220 cleavage products, in cell lysates from poliovirus-infected cells. The sedimentation of p220 antigens from uninfected or infected cells was analyzed by immunoblot and by enzyme-linked immunosorbent assay (ELISA) of sucrose gradient fractions. The results indicate that antibody reactivity was partially influenced by antigen conformation. Major forms of intact p220 and cleaved p220 were identified by immunoblot, and these had similar sedimentation properties. ELISA analysis of the same gradient fractions detected only uncleaved p220; p220 cleavage products were not recognized. Furthermore, the antibody recognized two forms of native uncleaved p220, one of which appeared to bind antibody with greater affinity. This result suggested the existence of conformational variants of p220. The differential reactivity of the antibody for cleaved versus uncleaved p220 served as a useful control during indirect immunofluorescence analysis to determine the subcellular distribution of p220 antigens. The distribution of p220 in uninfected cells was mainly cytoplasmic, but some nuclear antigens were also apparent. After poliovirus infection only the nuclear pattern remained. Disappearance of the cytoplasmic pattern confirmed the inability of the antibody to react with native p220 cleavage products. The cytoplasmic pattern also disappeared after human rhinovirus 14 infection, but not after mengovirus infection, results which correlated with the ability of human rhinovirus 14 and the inability of mengovirus to induce the cleavage of p220. The results demonstrate that p220 is not likely to be associated with the cytoskeleton and hint at the possibility of a partially nuclear location.
Distinct metabolite classes in root exudates are indicative for field- or hydroponically-grown cover crops (2023)

We found that hydroponically cultivated plants released a larger amount of total carbon, but that the recovery of total carbon was not indicative for the diversity of metabolites in root exudates. In the field, root exudates from phacelia and clover contained 2.4 to 3.8 times more secondary metabolites, whereas carbon exudation in hydroponics was 5- to 4-fold higher. The composition of the set of metabolites identified using the untargeted approach was much more distinct among all species and growth conditions than that of quantified primary metabolites. Among secondary metabolite classes, the presence of lipids and lipid-like molecules was highly indicative for field samples, while the release of a large amount of phenylpropanoids, organoheterocyclic compounds or benzenoids was characteristic for clover, mustard or oat, respectively, irrespective of the cultivation condition. However, at the compound level the bulk of released metabolites was specific for cultivation conditions in every species, which implies that hydroponically sampled root exudates poorly reflect the metabolic complexity of root exudates recovered from field-grown plants.
Mechanism of the Conversion of a Pulmonary Vein Tachycardia to Atrial Fibrillation in Normal Canine Hearts: Role of Autonomic Nerve Stimulation (2007)

These findings indicate that the vagal effects affecting the PVT and atria facilitate the onset and maintenance of PV-triggered AF in normal canine hearts.
Polyphenolics in Grape Seeds—Biochemistry and Functionality (2003)

Grape seeds are waste products of the winery and grape juice industry. These seeds contain lipid, protein, carbohydrates, and 5-8% polyphenols depending on the variety. Polyphenols in grape seeds are mainly flavonoids, including gallic acid, the monomeric flavan-3-ols catechin, epicatechin, gallocatechin, epigallocatechin, and epicatechin 3-O-gallate, and procyanidin dimers, trimers, and more highly polymerized procyanidins. Grape seed extract is known as a powerful antioxidant that protects the body from premature aging, disease, and decay. Grape seeds contains mainly phenols such as proanthocyanidins (oligomeric proanthocyanidins). Scientific studies have shown that the antioxidant power of proanthocyanidins is 20 times greater than vitamin E and 50 times greater than vitamin C. Extensive research suggests that grape seed extract is beneficial in many areas of health because of its antioxidant effect to bond with collagen, promoting youthful skin, cell health, elasticity, and flexibility. Other studies have shown that proanthocyanidins help to protect the body from sun damage, to improve vision, to improve flexibility in joints, arteries, and body tissues such as the heart, and to improve blood circulation by strengthening capillaries, arteries, and veins. The most abundant phenolic compounds isolated from grape seed are catechins, epicatechin, procyanidin, and some dimers and trimers.
Grain Oriented Silicon Steel Sheet with Ceramic Films Characterized by Ultra-Low Iron Loss (1992)

In order to investigate the possibility of improving the iron loss in grain oriented silicon steel sheet, ceramic coatings such as TiN, CrN and TiC were produced by ion plating on polished silicon steel sheets. The results obtained are summarized as follows.(1) The magnetic flux density B8(T) of silicon steel sheets with the ceramic films increases moderately to 0.004∼0.015 T, while the iron loss W17⁄50(W/kg) improves dramatically to 0.12∼0.20 W/kg. The improvement in the iron loss is remarkable in silicon steel sheets with higher magnetic flux densities. For example, in the silicon steel sheet products with B8=1.93 T, W17⁄50=0.88 W/kg, the final magnetic properties containing magnetic domain refinements manifest an ultra-low iron loss of 0.55 W/kg, which corresponds to the improvement in iron loss of about 40%.(2) Silicon steel sheets with TiN or CrN films are improved dramatically in magnetic properties, while those with TiC, SiC, SiO2, or Al2O3 films show comparatively a little improvement.(3) The attainment of ultra-low iron loss is due to improvement of hysteresis loss arising from the use of a polished steel surface which is clean and free from impurities and to the reduced eddy current loss arising from the strong tension of the ceramic films.(4) Silicon steel sheets with ceramic films show no deterioration in magnetic properties after strain relief annealing, superior compressive stress dependence of magnetstriction and good lamination performance.(5) It is considered that the ceramic coatings on the polished silicon steel sheet produced by physical techniques such as the PVD and CVD methods are the best method of improving the iron loss in silicon steel sheet.
The East Flanders Prospective Twin Survey (Belgium): a population–based register (1998)

The East Flanders Prospective Twin Survey (EFPTS), started in 1964, is unique among the 17 major European twin registers because it is population based, the twins (and higher order births) are ascertained at birth, basic perinatal data are collected, chorion type is established and, when appropriate, genetic markers including DNA fingerprints, are determined. The total number of sets is 5089 twin, 158 triplet and 14 of higher order. Zygosity has been diagnosed on the basis of sex, placental structure and genetic markers in more than 95% of pairs. The EFPTS is the only large register that includes placental data and allows differentiation of three subtypes of monozygotic twins based on the time of the initial zygotic division: the dichorionic-diamnionic pairs (early), the monochorionic-diamnionic pairs (intermediate), and the monochorionic-monoamnionic pairs (late). Methodology and basic results in twins are considered in this article; detailed studies will be reported later. The sex proportion in dizygotic (DZ) twins is the same as in singletons, whereas monozygotic (MZ) twins number more girls than boys. The difference in perinatal mortality between DZ and MZ twins is limited to the monochorionic MZ subgroup. Birth weight is highest in DZ twins and diminishes stepwise in MZ dichorionic and MZ monochorionic twins. Duration of pregnancy follows the same trend but is limited to a few days. Iatrogenic pregnancies are increasing to the point of representing almost 50% of the twin births in 1997.
ChemInform Abstract: FLUORINATION OF TRICHLORMETHIAZIDE WITH FLUOROXYTRIFLUOROMETHANE (1980)

Abstract Durch Einleiten von Fluoroxytrifluormethan in eine Lösung von Trichlormethiazid (I) wird das 5‐Fluorderivat (II) sowie das Diaziridin (III) und das Benzothiadiazindioxid (IV) erhalten.
Proto-oncogene Sno expression, alternative isoforms and immediate early serum response (1997)

The mouse Sno gene, a Ski proto-oncogene homolog, expresses two isoforms, SnoN and SnoN2 (also called sno -dE3), which differ from each other in a location downstream from the site of alternative splicing previously described in the human SNO gene. SnoN2 is missing a 138 nt coding segment present in mouse SnoN and human SNON . We have cloned and sequenced the human ortholog of mouse SnoN2 , the existence of which was predicted from conservation of the alternative splice donor site that produces the SnoN2 isoform. Mouse SnoN2 and SnoN are expressed throughout embryonic development, in neonatal muscle and in many adult tissues. SnoN2 is the major species in most tissues, but SnoN and SnoN2 are expressed at approximately equal levels in brain. In human tissues, SNON2 is the less abundantly expressed isoform. Expression of mouse SnoN and SnoN2 mRNAs is induced with immediate early kinetics upon serum stimulation of quiescent fibroblasts, even in the presence of the protein synthesis inhibitor cycloheximide, while Ski is not. Interestingly, although both isoforms of Sno are induced, SnoN2 induction is much higher than SnoN . These data are consistent with a role for Sno in the response to proliferation stimuli.
Super ionic conductive glass (2023)

An ionically conducting glass for use as a solid electrolyte in a power or secondary cell containing an alkali metal-containing anode and a cathode separated by an alkali metal ion conducting glass having an ionic transference number of unity and the general formula: A.sub.1+x D.sub.2-x/3 Si.sub.x P.sub.3-x O.sub.12-2x/3, wherein A is a network modifier for the glass and is an alkali metal of the anode, D is an intermediate for the glass and is selected from the class consisting of Zr, Ti, Ge, Al, Sb, Be, and Zn and X is in the range of from 2.25 to 3.0. Of the alkali metals, Na and Li are preferred and of the intermediate, Zr, Ti and Ge are preferred.
2,3-Dihydroxypropane-1-sulfonate degraded by Cupriavidus pinatubonensis JMP134: purification of dihydroxypropanesulfonate 3-dehydrogenase (2010)

2,3-Dihydroxypropane-1-sulfonate (DHPS) is a widespread intermediate in plant and algal transformations of sulfoquinovose (SQ) from the plant sulfolipid sulfoquinovosyl diacylglycerol. Further, DHPS is recovered quantitatively during bacterial degradation of SQ by Klebsiella sp. strain ABR11. DHPS is also a putative precursor of sulfolactate in e.g. Ruegeria pomeroyi DSS-3. A bioinformatic approach indicated that some 28 organisms with sequenced genomes might degrade DHPS inducibly via sulfolactate, with three different desulfonative enzymes involved in its degradation in different organisms. The hypothesis for Cupriavidus pinatubonensis JMP134 (formerly Ralstonia eutropha) involved a seven-gene cluster (Reut_C6093-C6087) comprising a LacI-type transcriptional regulator, HpsR, a major facilitator superfamily uptake system, HpsU, three NAD(P)(+)-coupled DHPS dehydrogenases, HpsNOP, and (R)-sulfolactate sulfo-lyase (SuyAB) [EC 4.4.1.24]. HpsOP effected a DHPS-racemase activity, and HpsN oxidized (R)-DHPS to (R)-sulfolactate. The hypothesis for Roseovarius nubinhibens ISM was similar, but involved a tripartite ATP-independent transport system for DHPS, HpsKLM, and two different desulfonative enzymes, (S)-cysteate sulfo-lyase [EC 4.4.1.25] and sulfoacetaldehyde acetyltransferase (Xsc) [EC 2.3.3.15]. Representative organisms were found to grow with DHPS and release sulfate. C. pinatubonensis JMP134 was found to express at least one NAD(P)(+)-coupled DHPS dehydrogenase inducibly, and three different peaks of activity were separated by anion-exchange chromatography. Protein bands (SDS-PAGE) were subjected to peptide-mass fingerprinting, which identified the corresponding genes (hpsNOP). Purified HpsN converted DHPS to sulfolactate. Reverse-transcription PCR confirmed that hpsNOUP were transcribed inducibly in strain JMP134, and that hpsKLM and hpsNOP were transcribed in strain ISM. DHPS degradation is widespread and diverse, implying that DHPS is common in marine and terrestrial environments.
Genetic algorithm based efficient positioning error compensation technique with low computational complexity (2009)

To alleviate positioning error using wireless ultra wideband (UWB) is primary concern, and it has been studied how to reduce the positioning error effectively. Thanks to many repeated transmissions of UWB signals, we can have a variety of selections to point out the most precise positioning result. Towards this, scanning method has been preferred to be used due to its simplicity. This exhaustive method firstly fixes the candidate position, and calculates the sum of distances from observed positions. However, it has tremendous number of computations, and the complexity is more serious if the size of two-dimensional range is the larger. To mitigate the large number of computations, this paper proposes the technique employing genetic algorithm. To exploit its superiority, simulations will be conducted to show the reduction of complexity, and the efficiency on positioning capability.
A PDMS-Based Integrated Stretchable Microelectrode Array (isMEA) for Neural and Muscular Surface Interfacing (2013)

Numerous applications in neuroscience research and neural prosthetics, such as electrocorticogram (ECoG) recording and retinal prosthesis, involve electrical interactions with soft excitable tissues using a surface recording and/or stimulation approach. These applications require an interface that is capable of setting up high-throughput communications between the electrical circuit and the excitable tissue and that can dynamically conform to the shape of the soft tissue. Being a compliant material with mechanical impedance close to that of soft tissues, polydimethylsiloxane (PDMS) offers excellent potential as a substrate material for such neural interfaces. This paper describes an integrated technology for fabrication of PDMS-based stretchable microelectrode arrays (MEAs). Specifically, as an integral part of the fabrication process, a stretchable MEA is directly fabricated with a rigid substrate, such as a thin printed circuit board (PCB), through an innovative bonding technology-via-bonding-for integrated packaging. This integrated strategy overcomes the conventional challenge of high-density packaging for this type of stretchable electronics. Combined with a high-density interconnect technology developed previously, this stretchable MEA technology facilitates a high-resolution, high-density integrated system solution for neural and muscular surface interfacing. In this paper, this PDMS-based integrated stretchable MEA (isMEA) technology is demonstrated by an example design that packages a stretchable MEA with a small PCB. The resulting isMEA is assessed for its biocompatibility, surface conformability, electrode impedance spectrum, and capability to record muscle fiber activity when applied epimysially.
Reinforcement learning for trading systems and portfolios (1998)

We propose to train trading systems by optimizing fi-nancial objective functions via reinforcement learning. The performance functions that we consider as value functions are profit or wealth, the Sharpe ratio and our recently proposed differential Sharpe ratio for on-line learning. In Moody &amp; Wu (1997), we presented empirical results in controlled experiments that demon-strated the advantages of reinforcement learning rela-tive to supervised learning. Here we extend our pre-vious work to compare Q-Learning to a reinforcement learning technique based on real-time recurrent learn-ing (RTRL) that maximizes immediate reward. Our simulation results include a spectacular demon-stration of the presence of predictability in the monthly