gebhart/patrepeval huggingface dataset:
Rapamycin Enhances the Anti-Cancer Effect of Dasatinib by Suppressing Src/PI3K/mTOR Pathway in NSCLC Cells (2015)

Src and the mammalian target of rapamycin (mTOR) signaling are commonly activated in non-small cell lung cancer (NSCLC) and hence potential targets for chemotherapy. Although the combined use of Src inhibitor Dasatinib with other chemotherapeutic agents has shown superior efficacy for cancer treatment, the mechanisms that lead to enhanced sensitivity of Dasatinib are not completely understood. In this study, we found that Rapamycin dramatically enhanced Dasatinib-induced cell growth inhibition and cell cycle G1 arrest in human lung adenocarcinoma A549 cells without affecting apoptosis. The synergistic effects were consistently correlated with the up-regulation of cyclin-dependent kinases inhibitor proteins, including p16, p19, p21, and p27, as well as the repression of Cdk4 expression and nuclear translocation. Mechanistic investigations demonstrated that FoxO1/FoxO3a and p70S6K/4E-BP1, the molecules at downstream of Src-PI3K-Akt and mTOR signaling, were significantly suppressed by the combined use of Dasatinib and Rapamycin. Restraining Src and mTOR with small interfering RNA in A549 cells further confirmed that the Src/PI3K/mTOR Pathway played a crucial role in enhancing the anticancer effect of Dasatinib. In addition, this finding was also validated by a series of assays using another two NSCLC cell lines, NCI-H1706 and NCI-H460. Conclusively, our results suggested that the combinatory application of Src and mTOR inhibitors might be a promising therapeutic strategy for NSCLC treatment.
Mirando El Cielo (1968-1969) (2016)

Este artículo se propone estudiar los tres números publicados de la revista El Cielo (1968-1969), dirigida conjuntamente por César Aira y Arturo Carrera, y presentar algunos rasgos que la ponen en diálogo con otros proyectos culturales y editoriales contemporáneos. La revista incluyó algunas colaboraciones literarias inéditas, traducciones, trabajos de crítica literaria y plástica, bajo un principio poético antes que comunicacional. En ese movimiento, El Cielo eligió ubicarse entre dos vanguardias, el surrealismo y la cultura pop. Esta elección se verifica también en las relaciones de afiliación estética que propone la revista. Todos estos elementos se desplegaron más tarde en los proyectos literarios que desarrollarían tanto Aira como Carrera.
Molecular Design of Light-Responsive Hydrogels, For in Situ Generation of Fast and Reversible Valves for Microfluidic Applications (2015)

Reversible light-responsive hydrogel valves with response characteristics compatible for microfluidics have been obtained by optimization of molecular design of spiropyran photoswitches and gel composition. Self-protonating gel formulations were exploited, wherein acrylic acid was copolymerized in the hydrogel network as an internal proton donor, to achieve a swollen state of the hydrogel in water at neutral pH. Light-responsive properties were endowed upon the hydrogels by copolymerization of spiropyran chromophores, using electron withdrawing and donating groups to tune the gel-swelling and shrinkage behavior. In all cases, the shrinkage was determined by the water diffusion rate, while for the swelling the isomerization kinetics is the rate-determining step. For one hydrogel, reversible and reproducible volume changes were observed. Finally, gel-valves integrated within microfluidic channels were fabricated, allowing reversible and repeatable operation, with opening and closing of the valve in minutes.
Rinderpest virus infection of bovine peripheral blood monocytes (1995)

The ability of rinderpest virus (RPV) to replicate in vitro in adherent peripheral blood monocytes and monocyte-derived macrophages under non-stimulation conditions was investigated. When flow cytometry analysis on bovine peripheral blood mononuclear cells (PBMC) was performed, monocytic cells were seen to be targets for infection by the cell culture-attenuated RBOK vaccine strain of RPV. Viral glycoprotein (H) and nucleoprotein (N) expression in adherent blood monocytes and monocyte-derived macrophages was compared with the infection in Vero cells, in which a productive infection typical of morbilliviruses is obtained. In both cell types, the infection was m.o.i.-dependent, but the rate of viral protein accumulation was slower in monocytes/macrophages. Double-labelling experiments with monoclonal antibodies against RPV and the myeloid marker CD14 confirmed that the infected blood adherent cells were monocytes and macrophages. Productive infection of monocytes was confirmed by progeny virus titration. Permissiveness to infection was not dependent on macrophage differentiation: in vitro maturation of monocytes to macrophages before infection, did not increase the susceptibility of these cells to RPV infection. With the virulent Saudi RPV isolate, similar results were obtained, although the Saudi virus apparently had a higher rate of replication compared to the attenuated virus. These observations demonstrate clearly that bovine blood monocytes and monocyte-derived macrophages serve as hosts for a relatively slow but productive infection by rinderpest virus.
Operating system support for execution time budgets for thread groups (2007)

The recent Ada 2005 standard introduced a number of new real-time services, with the capability of creating and managing execution time budgets for groups of tasks. This capability has many practical applications in real-time systems in general, and therefore it is also interesting for real-time operating systems. In this paper we present an implementation of thread group budgets inside a POSIX real-operating system, which can be used to implement the new Ada 2005 services. The architecture and details of the implementation are shown, as they may be useful to other implementers of this functionality defined in the new standard.
On the possibilities and limitations of rational protein design to expand the specificity of restriction enzymes: a case study employing EcoRV as the target (2000)

The restriction endonuclease EcoRV has been characterized in structural and functional terms in great detail. Based on this detailed information we employed a structure-guided approach to engineer variants of EcoRV that should be able to discriminate between differently flanked EcoRV recognition sites. In crystal structures of EcoRV complexed with d(CGGGATATCCC)(2) and d(AAAGATATCTT)(2), Lys104 and Ala181 closely approach the two base pairs flanking the GATATC recognition site and thus were proposed to be a reasonable starting point for the rational extension of site specificity in EcoRV [Horton,N.C. and Perona,J.J. (1998) J. Biol. Chem., 273, 21721-21729]. To test this proposal, several single (K104R, A181E, A181K) and double mutants of EcoRV (K104R/A181E, K104R/A181K) were generated. A detailed characterization of all variants examined shows that only the substitution of Ala181 by Glu leads to a considerably altered selectivity with both oligodeoxynucleotide and macromolecular DNA substrates, but not the predicted one, as these variants prefer cleavage of a TA flanked site over all other sites, under all conditions tested. The substitution of Lys104 by Arg, in contrast, which appeared to be very promising on the basis of the crystallographic analysis, does not lead to variants which differ very much from the EcoRV wild-type enzyme with respect to the flanking sequence preferences. The K104R/A181E and K104R/A181K double mutants show nearly the same preferences as the A181E and A181K single mutants. We conclude that even for the very well characterized restriction enzyme EcoRV, properties that determine specificity and selectivity are difficult to model on the basis of the available structural information.
SDN-Enabled Cross-Layer Flexibility in 5G Fronthaul Networks (2018)

We propose a software-defined resource allocation framework for mobile fronthaul (MFH) applications, combining secure and scalable modulation order and code rate adaptation and dynamic processing resource sharing. Successful transmission within a 34 km cover range is experimentally demonstrated.
Potential-field inversion: Choosing the appropriate technique to solve a geologic problem (2001)

Abstract To produce a unique and stable solution in potential-field interpretation, an inversion method must introduce particular constraints. These constraints will inevitably restrict the type of geological setting where the method may be applied. We present a nonmathematical overview of most stabilizing constraints used in inversion methods. Our purpose is to demonstrate that the inversion results are valuable only if the mathematical stabilizing constraints are translated from the geological setting. We identify five basic types of constraints: (1) lower and upper bounds of parameter estimates; (2) proximity of a parameter estimate to a specified value; (3) proximity between pairs of parameter estimates; (4) concentration of the anomalous source about a geometrical element such as an axis; and (5) source compactness. In practice, if used in isolation, constraints (1), (2), (4), and (5) will not produce geologically meaningful results, regardless of the geological setting of the interpretation area. Constraint (3) may produce geologically meaningful results if the anomalous source has a spatially smooth attribute such as the physical property. We illustrate that constraints 1–4, if used in isolation, cannot delineate the geometry of a simulated sill intruded into a sedimentary basin. The basic constraints may (and should) be combined in inversion to produce geologically meaningful results. We present two examples of effective constraint combination: (1) proximity to a specific value and mass concentration about an axis (used to delineate the thickness variation of a sill intruded in a sedimentary basin) and (2) inequality, proximity of a parameter estimate to a specified value, and proximity between pairs of parameter estimates (used to map a discontinuous basement relief). Usually, the stabilizing constraints are too restrictive to hold at all points of a given geological environment. In this case, we use different constraints in different subareas. Each constraint is based on its compatibility with the actual geology of the subarea.
Hy+ (1993)

article Free Access Share on Hy+: a Hygraph-based query and visualization system Authors: Mariano Consens Computer Systems Research Institute, University of Toronto, Toronto, Canada M5S 1A1 Computer Systems Research Institute, University of Toronto, Toronto, Canada M5S 1A1View Profile , Alberto Mendelzon Computer Systems Research Institute, University of Toronto, Toronto, Canada M5S 1A1 Computer Systems Research Institute, University of Toronto, Toronto, Canada M5S 1A1View Profile Authors Info & Claims ACM SIGMOD RecordVolume 22Issue 2June 1, 1993 pp 511–516https://doi.org/10.1145/170036.171537Online:01 June 1993Publication History 72citation610DownloadsMetricsTotal Citations72Total Downloads610Last 12 Months22Last 6 weeks4 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
Clostridium Collagenase Impact on Zone of Stasis Stabilization and Transition to Healthy Tissue in Burns (2021)

Clostridium collagenase has provided superior clinical results in achieving digestion of immediate and accumulating devitalized collagen tissue. Recent studies suggest that debridement via Clostridium collagenase modulates a cellular response to foster an anti-inflammatory microenvironment milieu, allowing for a more coordinated healing response. In an effort to better understand its role in burn wounds, we evaluated Clostridium collagenase's ability to effectively minimize burn progression using the classic burn comb model in pigs. Following burn injury, wounds were treated with Clostridium collagenase or control vehicle daily and biopsied at various time points. Biopsies were evaluated for factors associated with progressing necrosis as well as inflammatory response associated with treatment. Data presented herein showed that Clostridium collagenase treatment prevented destruction of dermal collagen. Additionally, treatment with collagenase reduced necrosis (HMGB1) and apoptosis (CC3a) early in burn injuries, allowing for increased infiltration of cells and protecting tissue from conversion. Furthermore, early epidermal separation and epidermal loss with a clearly defined basement membrane was observed in the treated wounds. We also show that collagenase treatment provided an early and improved inflammatory response followed by faster resolution in neutrophils. In assessing the inflammatory response, collagenase-treated wounds exhibited significantly greater neutrophil influx at day 1, with macrophage recruitment throughout days 2 and 4. In further evaluation, macrophage polarization to MHC II and vascular network maintenance were significantly increased in collagenase-treated wounds, indicative of a pro-resolving macrophage environment. Taken together, these data validate the impact of clostridial collagenases in the pathophysiology of burn wounds and that they complement patient outcomes in the clinical scenario.
Hardware Design of In-loop Filter for High Performance HEVC Encoder (2016)

본 논문에서는 고성능 HEVC(High Efficiency Video Coding) 부호기를 위한 루프 내 필터의 효율적인 하드웨어구조를 제안한다. HEVC는 양자화 에러가 발생하는 복원 영상에서 화질을 향상시키기 위해 디블록킹 필터와 SAO(Sample Adaptive Offset)으로 구성된 루프 내 필터를 사용한다. 그러나 루프 내 필터는 추가적인 연산으로 인하여 부호기와 복호기의 복잡도가 증가되는 원인이 된다. 제안하는 루프 내 필터 하드웨어 구조는 수행 사이클 감소를 위해 디블록킹 필터와 SAO를 3단 파이프라인으로 구현되었다. 또한 제안하는 디블록킹 필터는 6단 파이프라인 구조로 구현되었으며, 효율적인 참조 메모리 구조를 위해 새로운 필터링 순서로 수행된다. 제안하는 SAO는 화소들의 처리를 간소화하며 수행 사이클을 감소시키기 위해 한번에 6개의 화소를 병렬 처리된다. 제안하는 루프 내 필터 하드웨어 구조는 Verilog HDL로 설계되었으며, TSMC 0.13㎛ CMOS 표준 셀 라이브러리를 사용하여 합성한 결과 약 131K개의 게이트로 구현되었다. 또한 164㎒의 동작 주파수에서 4K@60fps의 실시간 처리가 가능하며, 최대 동작 주파수는 416㎒이다.
A novel one-step lens cleaning device using air and water flow for endoscopic surgery (2018)

In a surgical operation requiring endoscopy, it is essential to obtain a clear endoscopic view. However, it is often disturbed by the contamination on the lens during the surgery. No device can clean the lens surface simply and completely. Many surgeons are hampered by the impaired view and the distraction by the repeated cleaning of the lens. Therefore, we developed a novel endoscope cleaning device to address this problem. The device was made of 3D-printed rubber-like plastic. It contains a syringe filled with saline and an aspiration system. It would be used intraoperatively to wash the lens surface in a few seconds with rapid flow of water and air. The cleaning ability of the device was evaluated using mayonnaise with adenosine triphosphate (ATP) as a model contaminant. The gauze-wiping maneuver was selected as control. After each maneuver, the clarity of the endoscopic view was evaluated, and residual contaminants were assessed quantitatively with ATP assay. The cleaning device obtained a crisp and clear view and eliminated the contaminant on the lens every time after a single cleaning maneuver. The gauze-wiping maneuver required for the lens to be wiped at least three times to obtain a clear view, and even then, some contaminants remained. Repeated contamination and cleaning using gauze led to accumulation of contaminants on the lens, which resulted in difficulty in cleaning the lens as the operation proceeded. The cleaning device did not show such accumulation. Our novel cleaning device with air and water flow has been shown to wash out the lens contaminants completely and immediately in a simple manner. It is expected to improve the safety and cost-effectiveness of endoscopic surgery.
Trustworthiness of Medical Devices and Body Area Networks (2014)

Implantable and wearable medical devices (IWMDs) are commonly used for diagnosing, monitoring, and treating various medical conditions. A general trend in these medical devices is toward increased functional complexity, software programmability, and connectivity to body area networks (BANs). However, as IWMDs become more “intelligent,” they also become less trustworthy-less reliable and more prone to attacks. Various shortcomings-hardware failures, software errors, wireless attacks, malware and software exploits, and side-channel attacks-could undermine the trustworthiness of IWMDs and BANs. While these concerns have been recognized for some time, recent demonstrations of security attacks on commercial products, e.g., pacemakers and insulin pumps, have elevated medical device security from the realm of theoretical possibility to an immediate concern. The trustworthiness of IWMDs must be addressed aggressively and proactively due to the potential for catastrophic consequences. Conventional fault tolerance and information security solutions, e.g., redundancy and cryptography, that have been employed in general-purpose and embedded computing systems cannot be applied to many IWMDs due to their extreme size and power constraints and unique usage models. While several recent efforts address defense of IWMDs against specific security attacks, a holistic strategy that considers all concerns and types of threats is required. This paper discusses trustworthiness concerns in IWMDs and BANs through a comprehensive identification and analysis of potential threats and, for each threat, provides a discussion of the merits and inadequacies of current solutions.
Designing effective transfer functions for volume rendering from photographic volumes (2002)

Photographic volumes present a unique, interesting challenge for volume rendering. In photographic volumes, the voxel color is pre-determined, making color selection through transfer functions unnecessary. However, photographic data does not contain a clear mapping from the multi-valued color values to a scalar density or opacity, making projection and compositing much more difficult than with traditional volumes. Moreover, because of the nonlinear nature of color spaces, there is no meaningful norm for the multi-valued voxels. Thus, the individual color channels of photographic data must be treated as incomparable data tuples rather than as vector values. Traditional differential geometric tools, such as intensity gradients, density and Laplacians, are distorted by the nonlinear non-orthonormal color spaces that are the domain of the voxel values. We have developed different techniques for managing these issues while directly rendering volumes from photographic data. We present and justify the normalization of color values by mapping RGB values to the CIE L*u*v* color space. We explore and compare different opacity transfer functions that map three-channel color values to opacity. We apply these many-to-one mappings to the original RGB values as well as to the voxels after conversion to L*u*v* space. Direct rendering using transfer functions allows us to explore photographic volumes without having to commit to an a-priori segmentation that might mask fine variations of interest. We empirically compare the combined effects of each of the two color spaces with our opacity transfer functions using source data from the Visible Human project.
Reflection of an open-ended coaxial line and application to nondestructive measurement of materials (1981)

Reflection measurement techniques require a way to correlate measured reflection factors with the permittivity of the materials. This relationship is derived for the open-ended coaxial line propagating the TEM mode.
High rate and compact single sludge pre-denitrification process for retrofit (1994)

For the sewage treatment plants near rivers and closed water bodies in urbanized areas in Japan and European countries, there is a growing demand for introduction of advanced treatment processes for nitrogen and phosphorus from the viewpoints of water quality conservation and environmental protection. In order to remove nitrogen by the conventional biological treatment techniques, it is necessary to make a substantial expansion of the facility as compared with the conventional activated sludge process. In such urbanized districts, it is difficult to secure a site and much capital is required to expand the existing treatment plant. To solve these problems, a compact single sludge pre-denitrification process using immobilized nitrifiers was developed. Dosing the pellets, which are suitable for nitrifiers growth and physically durable, into the nitrification tank of single sludge pre-denitrification process made it possible to perform simultaneous removal of BOD and nitrogen in a retention time equal to that in the conventional activated sludge process even at the low water temperature of about 10 °C. The 3,000 m3/d full-scale conventional activated sludge plant was retrofitted and has been successfully operated.
Ground Station Network Optimization for Space-to-Ground Optical Communication Links (2015)

Space-to-ground optical data links enable higher data rates, require less electrical power, and allow more compact system designs than their corresponding RF counterparts. They may be applied to e.g. downlink earth observation data from low Earth orbit satellites, or as so-called feeder links for data transmission to geostationary telecommunication or multimedia satellites. However, optical space-to-ground links suffer from limited availability due to cloud blockage. The application of optical ground station (OGS) diversity and thus a network of OGS is required to overcome this limitation.
Study on Modification of Polymer Properties by the Cold Drawing Process (2015)

Polymers have a wide diversity of applications, ranging from therapeutics delivery to tissue engineering. While advances in polymer chemistry have facilitated synthesis and development of new polymers, increasing efforts have also been directed to engineer properties of existing polymers. One of the common approaches to modify polymer properties is cold drawing, which can align polymer chains and orient the chains in a crystalline manner. Regarding the industrial significance of cold drawing in polymer engineering, this study used semi-crystalline high density polyethylene (HDPE) as a model to examine the effect of cold drawing on the anisotropic mechanical properties of polymers. During cold drawing, the yield strength of the polymer was shown to be in a positive relationship with the strain rate, and the hardness of the cold-drawn region was demonstrated to be significantly enhanced. Our results confirmed the feasibility of engineering the properties of polymers by applying tension for plastic deformation, and highlighted the importance of precise control of the strain rate in the treatment.