gebhart/patrepeval huggingface dataset:
Nota crítica de los índices de Livi sobre el coste de vida del turista (2023)

El autor después de un análisis profundo llega a la conclusión de que puede considerarse que los índices elaborados por Livi (datos sobre el incremento del índice del coste de la vida) unen a fuertes dificultades teóricas, un método incorrecto, unos elevados errores de muestreo y sobre todo un desconocimiento de los datos de origen que hace que la validez de los índices se sustenten totalmente en la buena fe del profesor Livi, y puede ponerse en razonable duda. Puede observarse que las cifras dadas para España son excesivas, tanto en el año 1964, como, aún más, en el año 1965. Especial peso y significación tienen los costes en alojamientos y comidas.
PCR Detection and Microbiological Isolation of <i>Salmonella</i> spp. from Fresh Beef and Cantaloupes (2008)

Species belonging to the genus Salmonella are an important cause of enteric fevers, gastroenteritis, and septicemia, and the pathogens are commonly transmitted through contaminated food. In this study, polymerase chain reaction (PCR) amplification of a 287-bp region of the invA gene was compared to a microbiological technique to determine the presence of Salmonella in retail beef and in cantaloupe rinse samples. Both methods showed the same level of sensitivity, detecting 1 CFU/25 g of meat after enrichment for 24 h at 42 degrees C. The presence of Salmonella was determined in 50 commercial top sirloin beef samples that were not artificially inoculated. Three samples were positive by the microbiological method, and these samples and an additional sample were positive by the PCR. Both methods were also used to test surface rinses of cantaloupes collected from 4 farms in Nayarit, Mexico. Salmonella was detected by the microbiological method in 9 of 20 samples (45%), whereas the pathogen was detected by the PCR in 11 samples (55%). This study demonstrates the utility of the PCR targeting the invA gene to determine the presence of Salmonella spp. in beef and cantaloupe samples.
Deep classifier (2008)

Organizing Web search results into hierarchical categories facilitates users' browsing through Web search results, especially for ambiguous queries where the potential results are mixed together. Previous methods on search result classification are usually based on pre-training a classification model on some fixed and shallow hierarchical categories, where only the top-two-level categories of a Web taxonomy is used. Such classification methods may be too coarse for users to browse, since most search results would be classified into only two or three shallow categories. Instead, a deep hierarchical classifier must provide many more categories. However, the performance of such classifiers is usually limited because their classification effectiveness can deteriorate rapidly at the third or fourth level of a hierarchy. In this paper, we propose a novel algorithm known as Deep Classifier to classify the search results into detailed hierarchical categories with higher effectiveness than previous approaches. Given the search results in response to a query, the algorithm first prunes a wide-ranged hierarchy into a narrow one with the help of some Web directories. Different strategies are proposed to select the training data by utilizing the hierarchical structures. Finally, a discriminative naíve Bayesian classifier is developed to perform efficient and effective classification. As a result, the algorithm can provide more meaningful and specific class labels for search result browsing than shallow style of classification. We conduct experiments to show that the Deep Classifier can achieve significant improvement over state-of-the-art algorithms. In addition, with sufficient off-line preparation, the efficiency of the proposed algorithm is suitable for online application
Robust RNA-based in situ mutation detection delineates colorectal cancer subclonal evolution (2017)

Intra-tumor heterogeneity (ITH) is a major underlying cause of therapy resistance and disease recurrence, and is a read-out of tumor growth. Current genetic ITH analysis methods do not preserve spatial context and may not detect rare subclones. Here, we address these shortfalls by developing and validating BaseScope-a novel mutation-specific RNA in situ hybridization assay. We target common point mutations in the BRAF, KRAS and PIK3CA oncogenes in archival colorectal cancer samples to precisely map the spatial and morphological context of mutant subclones. Computational modeling suggests that subclones must arise sufficiently early, or carry a considerable fitness advantage, to form large or spatially disparate subclones. Examples of putative treatment-resistant cells isolated in small topographical areas are observed. The BaseScope assay represents a significant technical advance for in situ mutation detection that provides new insight into tumor evolution, and could have ramifications for selecting patients for treatment.
Selective vinyl cationic polymerization of monomers with two cationically polymerizable groups. III. 2‐vinyloxyethyl glycidyl ether: An epoxy‐functionalized vinyl ether (1987)

Abstract Cationic polymerization of 2‐vinyloxyethyl glycidyl ether (VEGE), a vinyl ether with an epoxy group, was conducted with various initiators in CH 2 Cl 2 in the temperature range from +15 to −78°C, and the possibility of its selective vinyl polymerization was investigated. BF 3 OEt 2 polymerized both vinyl and epoxy groups of VEGE to yield polymers partially insoluble in organic solvents. HI/I 2 , iodine, and CF 3 SO 3 H gave soluble, low‐molecular‐weight oligomers with epoxy pendants. 1 H‐NMR structural analysis of the oligomeric products showed that the epoxy/vinyl ratio of the pendants decreases in the order: 100% epoxy ∼ CF 3 SO 3 H > HI/I 2 ∼ I 2 ≫ BF 3 OEt 2 . Although HI/I 2 or iodine mainly polymerized the vinyl group, the reaction of the vinyl ether‐type growing end with an epoxy group of VEGE took place during the polymerization, so that the monomer conversion leveled off at about 40%.
Catalytic Decomposition of Hydrazine over α-Mo<sub>2</sub>C/γ-Al<sub>2</sub>O<sub>3</sub>Catalysts (2004)

A series of α-Mo2C/γ-Al2O3 catalysts with different Mo loadings were prepared by temperature-programmed reaction and characterized by X-ray diffraction, BET specific surface area determination, and NH3 temperature-programmed desorption. Their ability to decompose hydrazine was tested using a thruster and a microreactor. The catalytic activity for hydrazine decomposition of the fresh α-Mo2C/γ-Al2O3 catalyst is higher than that of the passivated α-Mo2C/γ-Al2O3 catalyst. The catalytic activity also decreased with the reaction time at 303 K, and the deactivation is mainly due to strongly adsorbed NHx species. Flushing with N2 at 773 K recovers the activity of the catalyst. It was shown that hydrazine decomposes to NH3 and N2 at low temperatures, and the produced NH3 dissociates into N2 and H2 at elevated temperatures.
Cardiovascular risk score in Rheumatoid Arthritis (2016)

QRISK-2 calculator is more appropriate as it takes RA, ethnicity, CKD, and Atrial fibrillation as factors in risk assessment score.
Structural elucidation of the Brucella melitensis M antigen by high-resolution NMR at 500 MHz (1987)

The Brucella M antigen from the species type strain Brucella melitensis 16M has been identified as a component of the cell wall lipopolysaccharide (LPS). O polysaccharide liberated from this LPS by mild acid hydrolysis exhibited M activity in serological tests and was shown to be a homopolymer of 4-formamido-4,6-dideoxy-alpha-D-mannopyranosyl residues arranged in an oligosaccharide repeating unit as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the native lipopolysaccharide. Structural analysis of the O polysaccharide by NMR methods was difficult due to apparent microheterogeneity of the repeating unit, which was in fact caused by the presence of rotational isomers of the N-formyl moiety. This problem was resolved by chemical modification of the polysaccharide to its amino and N-acetyl derivatives, the 500-MHz 1H and 125-MHz 13C NMR spectra of which could be analyzed in terms of a unique structure through application of pH-dependent beta-shifts and two-dimensional techniques that included COSY, relayed COSY, and NOESY experiments together with heteronuclear C/H shift correlation spectroscopy. On the basis of these experiments and supported by methylation and periodate oxidation data, the structure of the M polysaccharide was determined as a linear polymer of unbranched pentasaccharide repeating units consisting of four 1,2-linked and one 1,3-linked 4,6-dideoxy-4-formamido-alpha-D-mannopyranosyl residues. The marked structural similarity of the M antigen and the A antigen, which is known to be a 1,2-linked homopolysaccharide of 4,6-dideoxy-4-formamido-alpha-D-mannopyranosyl units, accounts for cross-serological reactions of the two and the long-standing confusion surrounding the nature of their antigenic determinants. Structural and serological considerations in conjuction with the sodium dodecyl sulfate banding pattern of Brucella A LPS suggest that its biosynthesis differs appreciably from that of the M antigen, which appears to be synthesized by regulated assembly of preformed oligosaccharide repeating units. Temperature, lysogenic phage may be responsible for such biosynthetic and structural variations.
Imaging and modelling of digestion in the stomach and the duodenum (2006)

Gastroduodenal physiology is traditionally understood in terms of motor-secretory functions and their electrical, neural and hormonal controls. In contrast, the fluid-mechanical functions that retain and disperse particles, expose substrate to enzymes, or replenish the epithelial boundary with nutrients are little studied. Current ultrasound and magnetic resonance imaging allows to visualize processes critical to digestion like mixing, dilution, swelling, dispersion and elution. Methodological advances in fluid mechanics allow to numerically analyse the forces promoting digestion. Pressure and flow fields, the shear stresses dispersing particles or the effectiveness of bolus mixing can be computed using information on boundary movements and on the luminal contents. These technological advances promise many additional insights into the mechanical processes that promote digestion and absorption.
miR-223-3p Inhibits Human Osteosarcoma Metastasis and Progression by Directly Targeting CDH6 (2018)

Cadherin-6 (CDH6) is aberrantly expressed in cancer and closely associated with tumor progression. However, the functions of CDH6 in human osteosarcoma and the molecular mechanisms underlying CDH6 in osteosarcoma oncogenesis remain poorly understood. In this work, we assessed the role of CDH6 in human osteosarcoma and identified that the expression of CDH6 was closely related with the overall survival and poor prognosis of osteosarcoma patients. MicroRNAs (miRNAs) have been implicated as important epigenetic regulators during the progression of osteosarcoma. Using dual-luciferase reporter assays, we showed that miR-223-3p suppresses CDH6 expression by directly binding to the 3' UTR of CDH6. miR-223-3p overexpression significantly inhibited cell invasion, migration, growth, and proliferation by suppressing the CDH6 expression in vivo and in vitro. Besides, CDH6 overexpression in the miR-223-3p-transfected osteosarcoma cells effectively rescued the inhibition of cell invasion, migration, growth, and proliferation mediated by miR-223-3p. Additionally, Kaplan-Meier analysis suggests that the expression of miR-223-3p predicts favorable clinical outcomes for osteosarcoma patients. Moreover, the expression of miR-223-3p was downregulated in osteosarcoma patients and was negatively associated with the expression of CDH6. Collectively, these data highlight that miR-223-3p/CDH6 axis is an important novel pleiotropic regulator and could early predict the metastatic potential in human osteosarcoma treatments.
Inhibition of proteolysis and cell cycle progression in a multiubiquitination-deficient yeast mutant. (1994)

The degradation of many proteins requires their prior attachment to ubiquitin. Proteolytic substrates are characteristically multiubiquitinated through the formation of ubiquitin-ubiquitin linkages. Lys-48 of ubiquitin can serve as a linkage site in the formation of such chains and is required for the degradation of some substrates of this pathway in vitro. We have characterized the recessive and dominant effects of a Lys-48-to-Arg mutant of ubiquitin (UbK48R) in Saccharomyces cerevisiae. Although UbK48R is expected to terminate the growth of Lys-48 multiubiquitin chains and thus to exert a dominant negative effect on protein turnover, overproduction of UbK48R in wild-type cells results in only a weak inhibition of protein turnover, apparently because the mutant ubiquitin can be removed from multiubiquitin chains. Surprisingly, expression of UbK48R complements several phenotypes of polyubiquitin gene (UB14) deletion mutants. However, UbK48R cannot serve as a sole source of ubiquitin in S. cerevisiae, as evidenced by its inability to rescue the growth of ubi1 ubi2 ubi3 ubi4 quadruple mutants. When provided solely with UbK48R, cells undergo cell cycle arrest with a terminal phenotype characterized by replicated DNA, mitotic spindles, and two-lobed nuclei. Under these conditions, degradation of amino acid analog-containing proteins is severely inhibited. Thus, multiubiquitin chains containing Lys-48 linkages play a critical role in protein degradation in vivo.
Modeling Of Tow Wrinkling In Automated Fiber Placement Based On Geometrical Considerations (2017)

Automated manufacturing of fiber reinforced composite structures via numerically controlled hardware yields parts with increased accuracy and repeatability as compared to hand-layup parts. Automated fiber placement (AFP) is one such process in which structures or parts are built by adding bands of prescribed number of tows or slit-tape with prescribed width using robotic machine heads over 3D surfaces following prescribed paths. Despite the improved accuracy, different types of defects or manufacturing features arise during fabrication. These defects can be due to geometrical features, materials, and process planning parameters and are detected in the form of wrinkling, tow twist, tow folding, overlap, gaps and several others. This thesis presents a thorough investigation of wrinkling within a path on a general surface for a composite tow constructed using the AFP process. Governing equations and assumptions for the presented model are derived based on geometric considerations only, neglecting the elastic properties of the material, and formulated for an arbitrary curve on a general three-dimensional surface. A simple form of the wrinkled shape is assumed and applied to the inner edge of the tow path. A numerical solution is implemented within Mathematica to visualize the curved paths and to indicate potential regions for wrinkling on the surface. Several examples are presented to demonstrate the model, including constant angle paths on a double-curved surface and curved paths on a flat surface. The obtained deformed patterns are compared with actual data from digital image correlation (DIC) of several towpaths.
Total Synthesis of (−)‐Virginiamycin M<sub>2</sub> (2010)

Has a nice ring to it: A concise and modular total synthesis of the naturally occurring antibiotic virginiamycin M2 is described. A Barbier-type cyclization was used to close the 23-membered macrocycle and deliver virginiamycin M2 in 19 steps from a chiral organosilane.
Characterization of a New Arabidopsis Mutant Exhibiting Enhanced Disease Resistance (1999)

In many plant-pathogen interactions, resistance is associated with the synthesis and accumulation of salicylic acid (SA) and pathogenesis-related (PR) proteins. At least two general classes of mutants with altered resistance to pathogen attack have been identified in Arabidopsis. One class exhibits increased susceptibility to pathogen infection; the other class exhibits enhanced resistance to pathogens. In an attempt to identify mutations in resistance-associated loci, we screened a population of T-DNA tagged Arabidopsis thaliana ecotype Wassilewskija (Ws) for mutants showing constitutive expression of the PR-1 gene (cep). A mutant was isolated and shown to constitutively express PR-1, PR-2, and PR-5 genes. This constitutive phenotype segregated as a single recessive trait in the Ws genetic background. The mutant also had elevated levels of SA, which are responsible for the cep phenotype. The cep mutant spontaneously formed hypersensitive response (HR)-like lesions on the leaves and cotyledons and also exhibited enhanced resistance to virulent bacterial and fungal pathogens. Genetic analyses of segregating progeny from outcrosses to other ecotypes unexpectedly revealed that alterations in more than one gene condition the constitutive expression of PR genes in the original mutant. One of the mutations, designated cpr20, maps to the lower arm of chromosome 4 and is required for the cep phenotype. Another mutation, which has been termed cpr21, maps to chromosome 1 and is often, but not always, associated with this phenotype. The recessive nature of the cep trait suggests that the CPR20 and CPR21 proteins may act as negative regulators in the disease resistance signal transduction pathway.
4. Age dependence of the multiplicity of Plasmodium falciparum infections and of other malariological indices in an area of high endemicity (1999)

The relationship between age and various malariological indices in the Kilombero valley of Tanzania were examined by compiling data from 6 different community studies carried out between 1989 and 1996. The rate of acquisition of Plasmodium falciparum infection was highest in children 1-5 years of age, while recovery rates were lowest between the first birthday and early adolescence. As a result, peak prevalence was reached in 3-5 years old children. However, the prevalence of clinical malaria (estimated from the excess risk of axillary temperatures > or = 37.5 degrees C attributable to parasitaemia) was highest in children under one year of age. The peak in multiplicity of infection (identified by polymerase chain reaction-restriction fragment length polymorphism of the msp2 locus) occurred in 3-7 years old children. There was a significant correlation between parasite density and multiplicity of infection in infants and young children (1-2 years of age) but not in older individuals.
Mass Spectrometry Provides Accurate Characterization of two Genetic Marker Types in <i>Bacillus Anthracis</i> (2004)

Epidemiological and forensic analyses of bioterrorism events involving Bacillus anthracis could be improved if both variable number tandem repeats (VNTRs) and single nucleotide polymorphisms (SNPs) could be combined on a single analysis platform. Here we present the use of electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI-FTICR-MS) to characterize 24 alleles from 6 VNTR loci and 11 alleles from 7 SNP loci in B. anthracis. The results obtained with ESI-FTICR-MS were consistent with independent results obtained from traditional approaches using electrophoretic detection of fluorescent products. However, ESI-FTICR-MS improves on the traditional approaches because it does not require fluorescent labeling of PCR products, minimizes post-PCR processing, obviates electrophoresis, and provides unambiguous base composition of both SNP and VNTR PCR products. In addition, ESI-FTICR-MS allows both marker types to be examined simultaneously and at a rate of approximately 1 sample per min. This technology represents a significant advance in our ability to rapidly characterize B. anthracis isolates using VNTR and SNP loci.
Advanced methods in scanning x-ray microscopy (2009)

New developments in X-ray instrumentation and analysis have facilitated the development and improvement of various scanning X-ray microscopy techniques. In this contribution, we offer an overview of recent scanning hard X-ray microscopy measurements performed at the Swiss Light Source. We discuss scanning transmission X-ray microscopy in its transmission, phase contrast, and dark-field imaging modalities. We demonstrate how small-angle X-ray scattering analysis techniques can be used to yield additional information. If the illumination is coherent, coherent diffraction imaging techniques can be brought to bear. We discuss how, from scanning microscopy measurements, detailed measurements of the X-ray scattering distributions can be used to extract high-resolution images. These microscopy techniques with their respective imaging power can easily be combined to multimodal X-ray microscopy.
Aspartate 313 in the Streptomyces plicatusHexosaminidase Plays a Critical Role in Substrate-assisted Catalysis by Orienting the 2-Acetamido Group and Stabilizing the Transition State (2002)

SpHex, a retaining family 20 glycosidase from Streptomyces plicatus, catalyzes the hydrolysis of N-acetyl-beta-hexosaminides. Accumulating evidence suggests that the hydrolytic mechanism involves substrate-assisted catalysis wherein the 2-acetamido substituent acts as a nucleophile to form an oxazolinium ion intermediate. The role of a conserved aspartate residue (D313) in the active site of SpHex was investigated through kinetic and structural analyses of two variant enzymes, D313A and D313N. Three-dimensional structures of the wild-type and variant enzymes in product complexes with N-acetyl-d-glucosamine revealed substantial differences. In the D313A variant the 2-acetamido group was found in two conformations of which only one is able to aid in catalysis through anchimeric assistance. The mutation D313N results in a steric clash in the active site between Asn-313 and the 2-acetamido group preventing the 2-acetamido group from providing anchimeric assistance, consistent with the large reduction in catalytic efficiency and the insensitivity of this variant to chemical rescue. By comparison, the D313A mutation results in a shift in a shift in the pH optimum and a modest decrease in activity that can be rescued by using azide as an exogenous nucleophile. These structural and kinetic data provide evidence that Asp-313 stabilizes the transition states flanking the oxazoline intermediate and also assists to correctly orient the 2-acetamido group for catalysis. Based on analogous conserved residues in the family 18 chitinases and family 56 hyaluronidases, the roles played by the Asp-313 residue is likely general for all hexosaminidases using a mechanism involving substrate-assisted catalysis.
Dopamine and Migraine: A Review of Pharmacological, Biochemical, Neurophysiological, and Therapeutic Data (1998)

The dopamine theory of migraine pathogenesis, first proposed by F. Sicuteri in 1977, has attracted renewed interest after an increased frequency of the dopamine D2 receptor (DRD2) gene allele NcoI C was found in patients with migraine with aura. Therefore we reviewed the relevant literature. The most compelling argument favoring an interictal hypersensitivity of dopamine receptors in migraineurs stems from pharmacologic studies of the gastric and autonomic effects of dopaminergic agents such as apomorphine, but none of these studies was blinded and placebo-controlled. Various DRD2 antagonists abort migraine attacks after parenteral administration, while there is circumstantial evidence that dopamine agonists may be useful for prophylaxis. Most drugs used in these trials, however, lack selectivity for dopamine receptors. Both in pharmacological and therapeutic studies most patients had migraine without aura. We conclude that data suggesting a primary role for the dopaminergic system in migraine pathogenesis are unconvincing. Based on well established interactions between central amines, a reduced release of serotonin between attacks could lower dopamine release which would lead to receptor hypersensitivity.