gebhart/patrepeval huggingface dataset:
Influences of Transporter Associated with Antigen Processing (TAP) on the Repertoire of Peptides Associated with the Endoplasmic Reticulum–resident Stress Protein gp96 (1997)

The endoplasmic reticulum (ER)-resident stress protein gp96 induces protective immunity and specific cytotoxic T lymphocyte (CTL) responses against antigens expressed in those cells it has been isolated from. This ability is based on peptides associated with gp96. Because gp96 is located inside the ER, our experiments address the question whether or not the repertoire of peptides associated with gp96 is influenced by the transporter associated with antigen processing (TAP). For this purpose, gp96 was isolated from cells with and without a TAP defect and used for immunization of mice. We found that for some antigens the association of peptides with gp96 required functional TAP molecules, whereas the association of peptides from other antigens was TAP independent. In the case of a TAP-dependent association of peptides with gp96, our results prove that peptide binding by gp96 in vivo occurs inside the ER and is not an artifact induced by cell lysis during the gp96 purification. The finding that some antigens can also associate with gp96 in the absence of functional TAP molecules indicates that the repertoire of peptides bound by gp96 truly reflects the entire repertoire of peptides present inside the ER and not only those peptides transported by TAP. These results, together with the earlier finding that the gp96 peptide repertoire is independent of the major histocompatibility complex molecules expressed by the cell gp96 is isolated from, give the theoretical foundation for the ability of gp96 to induce CTL responses against all kinds of intracellular antigens.
SNOMED CT coding variation and grouping for "other findings" in a longitudinal study on urea cycle disorders. (2008)

Semantic interoperability requires consistent use of controlled terminologies. However, non-terminology experts (although perhaps experts in a particular domain) are prone to produce variant coding. We examine this problem by investigating SNOMED CT coding variation for other findings reported on case report forms from a clinical research study on urea cycle disorders. The natural language findings from the forms were normalized, and the associated SNOMED CT concept descriptions were compared. The subset of normalized strings associated with two different concept descriptions were further compared to determine the relationship among the associated SNOMED CT concepts. We found 45% of the concept description pairs were associated with two hierarchically related concepts or with the same concept, while 55% were associated with two unrelated concepts. Clearer guidelines for use of SNOMED CT in particular contexts, or structured data entry tools tailored to the needs of non-expert coders, are needed to better manage coding variation.
Altering Metabolic Profiles of Drugs by Precision Deuteration 2: Discovery of a Deuterated Analog of Ivacaftor with Differentiated Pharmacokinetics for Clinical Development (2017)

Ivacaftor is currently used for the treatment of cystic fibrosis as both monotherapy (Kalydeco; Vertex Pharmaceuticals, Boston, MA) and combination therapy with lumacaftor (Orkambi; Vertex Pharmaceuticals). Each therapy targets specific patient populations: Kalydeco treats patients carrying one of nine gating mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) protein, whereas Orkambi treats patients homozygous for the F508del CFTR mutation. In this study, we explored the pharmacological and metabolic effects of precision deuteration chemistry on ivacaftor by synthesizing two novel deuterated ivacaftor analogs, CTP-656 (<i>d</i><sub>9</sub>-ivacaftor) and <i>d</i><sub>18</sub>-ivacaftor. Ivacaftor is administered twice daily and is extensively converted in humans to major metabolites M1 and M6; therefore, the corresponding deuterated metabolites were also prepared. Both CTP-656 and <i>d</i><sub>18</sub>-ivacaftor showed in vitro pharmacologic potency similar to that in ivacaftor, and the deuterated M1 and M6 metabolites showed pharmacology equivalent to that in the corresponding metabolites of ivacaftor, which is consistent with the findings of previous studies of deuterated compounds. However, CTP-656 exhibited markedly enhanced stability when tested in vitro. The deuterium isotope effects for CTP-656 metabolism (<sup>D</sup>V = 3.8, <sup>D</sup>V/K = 2.2) were notably large for a cytochrome P450-mediated oxidation. The pharmacokinetic (PK) profile of CTP-656 and <i>d</i><sub>18</sub>-ivacaftor were assessed in six healthy volunteers in a single-dose crossover study, which provided the basis for advancing CTP-656 in development. The overall PK profile, including the 15.9-hour half-life for CTP-656, suggests that CTP-656 may be dosed once daily, thereby enhancing patient adherence. Together, these data continue to validate deuterium substitution as a viable approach for creating novel therapeutic agents with properties potentially differentiated from existing drugs.
A combined interval and floating-point divider (2002)

Interval arithmetic provides an efficient method for monitoring and controlling errors in numerical calculations. However existing software packages for interval arithmetic are often too slow for numerically intensive calculations. This paper presents the design of a divider that performs either interval or floating-point division. This divider requires only slightly more area than a conventional floating point divider and provides a significant performance improvement over software implementations of interval division.
Gestão e motivação na percepção dos funcionários da escola: um estudo de 2012 a 2022 (2023)

Gratidão a toda a equipe da "Escola Girassol", vocês foram fundamentais para o sucesso dessa pesquisa!Quanto aprendizado vivenciei com vocês!Por fim
EFFECT OF PORCINE ENDOTHELIAL TISSUE FACTOR PATHWAY INHIBITOR ON HUMAN COAGULATION FACTORS1 (1997)

Lack of appropriate human factor Xa inhibition by porcine EC during hyperacute rejection and loss of porcine EC TFPI during DXR could promote the development of a procoagulant environment leading to xenograft rejection.
Research and Implementation of Database Optimization and Integration for Power Enterprises Information System (2013)

In order to ensure security and stability of information systems in central China subsection of State Grid, and effectively support the construction of Three Intensive Management and Five Large-scale Movements management system, this paper analyzes the situations and problems of information system databases, and discusses the methods for optimizing and integrating the database using the existing software and hardware resources. After sufficiently surveying and testing the mainstream Oracle database migration technology and framework, this paper proposes an optimal integration technique solution and concludes its practical effects.
Dilated Cardiomyopathy Associated With Hepatitis C Virus Infection (1995)

The results suggest that hepatitis C virus infection is frequently found in patients with dilated cardiomyopathy and that hepatitis C virus is an important causal agent in the pathogenesis of the disease. Antiviral therapy against hepatitis C virus may be indicated in these patients.
Pyrosequencing Analysis of the Microbial Diversity of Airag, Khoormog and Tarag, Traditional Fermented Dairy Products of Mongolia (2014)

Here, we used pyrosequencing to obtain a detailed analysis of the microbial diversities of traditional fermented dairy products of Mongolia. From 22 Airag (fermented mare's milk), 5 Khoormog (fermented camel's milk) and 26 Tarag (fermented milk of cows, goats and yaks) samples collected in the Mongolian provinces of Arhangai, Bulgan, Dundgobi, Tov, Uburhangai and Umnugobi, we obtained a total of 81 operational taxonomic units, which were assigned to 15 families, 21 genera and 41 species in 3 phyla. The genus Lactobacillus is a core bacterial component of Mongolian fermented milks, and Lactobacillus helveticus, Lactobacillus kefiranofaciens and Lactobacillus delbrueckii were the predominant species of lactic acid bacteria (LAB) in the Airag, Khoormog and Tarag samples, respectively. By using this pyrosequencing approach, we successfully detected most LAB species that have been isolated as well as seven LAB species that have not been found in our previous culture-based study. A subsequent analysis of the principal components of the samples revealed that L. delbrueckii, L. helveticus, L. kefiranofaciens and Streptococcus thermophilus were the main factors influencing the microbial diversity of these Mongolian traditional fermented dairy products and that this diversity correlated with the animal species from which the milk was sourced.
Clinical effects of Garcinia kola in knee osteoarthritis (2008)

Garcinia kola appeared to have clinically significant analgesic/anti-inflammatory effects in knee osteoarthritis patients. Garcinia kola is a potential osteoarthritis disease activity modifier with good mid term outcome. Further studies are required for standardization of dosages and to determine long-term effects.
Spectrum reconstruction for filter-array spectrum sensor from sparse template selection (2011)

In recent years, miniature spectrometers have been found to be useful in many applications to resolve spectrum signatures of materials. In this paper, algorithms are proposed to realize a miniature spectrometer using a low-cost filter-array spectrum sensor. Conventionally, the filter-array spectrum sensor can be modeled as an overdetermined problem, and the spectrum can be reconstructed by solving a set of linear equations. In this paper, we instead model the spectrum reconstruction process as an underdetermined problem, and bring up the concept of template-selection by sparse representation. l1 − norm minimization is introduced to achieve a high reconstruction resolution. Both simulation and experimental results show that a superior quality of spectrum reconstruction can be made possible from the presented underdetermined approach.
Experimental Investigation of High Step-Up DC-DC Converter for Fuel Cell System (2012)

This work aims to present experimental investigation of a high step-up DC-DC converter for fuel cell system. The proposed converter comprises of an input-current doubler, an output-voltage doubler, and an active-clamp circuit. The input-current doubler and the output-voltage doubler both provide much higher voltage conversion ratio by using a low turn ratio transformer. The low turn ratio increases the overall efficiency. A series resonant circuit of the output voltage doubler removes the reverse recovery problem of the rectifying diodes. The active-clamp circuit clamps the surge voltage of switches and recycles the energy stored in the leakage inductance of the transformer. Simulation has been carried out for the proposed converter with the help of OrCAD software. Experimental results show that the better performance of the converter is achieved at an input voltage of 30V.
3-D Active Antenna for contact sensing (2002)

This paper discusses a 3-D Active Antenna that can detect the contact location between an insensitive flexible beam and a 3-D environment through the measurement of the rotational compliance of the beam in contact with the environment. The lateral slip, which possibly occurs for the 3-D Active Antenna, overestimates the rotational compliance, and as a result, brings a large sensing error for the localizing contact point. The goal of this paper is to find the contact point under such conditions. In the first step, we push the antenna to the environment. If no lateral slip is detected during this motion, the contact point can be obtained through a similar method used for the 2-D model. If a lateral slip is confirmed, the pushing direction is changed continuously until development of any lateral slip is finally avoided. The authors explore how to detect a lateral slip and how to determine the new pushing direction to avoid it. The authors show an algorithm which can search not only the contact distance but also the normal direction of the environment's surface, even under the appearance of a lateral slip during the first step. The basic idea is also verified by experiments.
Cryogenic Qubit Integration for Quantum Computing (2018)

We have demonstrated superconducting interconnect technologies that enable a variety of flip-chip 3D integrated structures and packages compatible with high-coherence superconducting qubits. Superconducting indium micro-bumps and underbump metal (UBM) were used to join superconducting qubit chips to superconducting readout and control modules while maintaining high qubit coherence (T <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">1</sub> , T <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> , <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">echo</sub> > 20 μs) in the presence of capacitive and inductive coupling between the chips. Scanning electron microscope, X-ray, infrared and confocal microscopy were used to investigate the micro-structure, alignment accuracy, and parallelism of flip-chip qubits. The superconducting readout and control modules can accommodate both niobium and aluminum-based circuit and amplifier fabrication processes, including shadow-evaporated aluminum or Nb/Al-AlOx/Nb trilayer Josephson junctions (JJs). We present results for up to 16 active superconducting chips having trilayer junctions bonded to a passive superconducting module. The I-V characteristics and switching behavior were measured for flip-chip-connected JJ arrays with 40-20,000 JJs. Our approach maintained chip-level junction critical current and qubit coherence, demonstrating it to be a viable approach to building larger quantum computing systems. This paper also discusses packaging approaches to developing a quantum-to-classical interface in a cryogenic environment with multiple temperature stages.
Multimedia streaming using multiple TCP connections (2005)

As broadband Internet becomes widely available, multimedia applications over the Internet become increasingly popular. However, packet loss, delay, and time-varying bandwidth of the Internet have remained the major problems for multimedia streaming applications. As such, a number of approaches, including network infrastructure and protocol, source and channel coding have been proposed to either overcome or alleviate these drawbacks of the Internet. In this paper, we propose the MultiTCP system, a receiver-driven, TCP-based system for multimedia streaming over the Internet. Our proposed algorithm aims at providing resilience against SHORT TERM insufficient bandwidth by using MULTIPLE TCP connections for the same application. Furthermore, our proposed system enables the application to achieve and control the desired sending rate during congested periods, which cannot be achieved using traditional TCP. Finally, our proposed system is implemented at the application layer, and hence, no kernel modification to TCP is necessary. We analyze the proposed system, and present simulation results to demonstrate its advantages over the traditional single TCP based approach.
The role of attorney mental models of law in case relevance determinations: An exploratory analysis (1994)

This article examines the information seeking and evaluative behavior of attorneys as they search the corpus of law for primary authority in order to solve context sensitive legal issues. First, the dynamic mental models attorneys construct of the law as expressed in its published artifacts is explored. The relevance judgment of cases is then explicated in terms of these models. The conclusion reached is that relevance judgments shift along a knowledge continuum depending on the status of the attorney's mental model, and that the factors underlying these judgments are complex, multidimensional, and knowable. Current empirical research into the retrieval effectiveness of two full-text legal databases is evaluated in light of the behavioral theory and mental models developed. The implications of attorney information seeking behavior for future information retrieval system design for this domain are also explored. © 1994 John Wiley & Sons, Inc.
Abstract 14000: ApoB-100 Related Peptide Vaccine Reduces Hypertension and Mortality in a Model of Angiotensin II-Induced Aortic Aneurysm in Apo E (-/-) Mice: A Novel Therapeutic Paradigm (2011)

Background: Aortic aneurysms (AA) are characterized by increased oxidative stress with chronic immune-mediated inflammation of the aortic wall. We recently demonstrated that immunization with apoB-...
Global Gene Expression in <i>Staphylococcus aureus</i> Biofilms (2004)

We previously demonstrated that mutation of the staphylococcal accessory regulator (sarA) in a clinical isolate of Staphylococcus aureus (UAMS-1) results in an impaired capacity to form a biofilm in vitro (K. E. Beenken, J. S. Blevins, and M. S. Smeltzer, Infect. Immun. 71:4206-4211, 2003). In this report, we used a murine model of catheter-based biofilm formation to demonstrate that a UAMS-1 sarA mutant also has a reduced capacity to form a biofilm in vivo. Surprisingly, mutation of the UAMS-1 ica locus had little impact on biofilm formation in vitro or in vivo. In an effort to identify additional loci that might be relevant to biofilm formation and/or the adaptive response required for persistence of S. aureus within a biofilm, we isolated total cellular RNA from UAMS-1 harvested from a biofilm grown in a flow cell and compared the transcriptional profile of this RNA to RNA isolated from both exponential- and stationary-phase planktonic cultures. Comparisons were done using a custom-made Affymetrix GeneChip representing the genomic complement of six strains of S. aureus (COL, N315, Mu50, NCTC 8325, EMRSA-16 [strain 252], and MSSA-476). The results confirm that the sessile lifestyle associated with persistence within a biofilm is distinct by comparison to the lifestyles of both the exponential and postexponential phases of planktonic culture. Indeed, we identified 48 genes in which expression was induced at least twofold in biofilms over expression under both planktonic conditions. Similarly, we identified 84 genes in which expression was repressed by a factor of at least 2 compared to expression under both planktonic conditions. A primary theme that emerged from the analysis of these genes is that persistence within a biofilm requires an adaptive response that limits the deleterious effects of the reduced pH associated with anaerobic growth conditions.
Effect of Carbon Content on the Mechanical Properties of Medium Carbon Steels (2010)

The mechanical properties of medium-carbon steels with a carbon content ranging from 0.30 to 0.55 wt.% were investigated by tensile and microhardness tests at room temperature. It was observed that the higher carbon content results in an increase in yield stress and ultimate tensile stress, while the elongation remains essentially constant. The results were explained by the hindering of dislocation motion associated with solid solution hardening
An antislipping fuzzy logic controller for a railway traction system (2002)

A controller has been designed to avoid the wheel-rail slipping in a scale model of a train. This scale model is being employed because of the difficulties in testing this nonlinear phenomenon in a real locomotive. The controller uses rules based on the knowledge about the slipping behavior of a skilled human operator; it has been developed using a fuzzy logic approach. Different simulations have shown that this fuzzy antislipping controller works satisfactorily decreasing the torque to be applied to the electrical drive when the slipping situation has been detected, and reducing both the slipping value and the time spent in recovery adherence.
Higher Pericyte Content and Secretory Activity of Microfragmented Human Adipose Tissue Compared to Enzymatically Derived Stromal Vascular Fraction (2018)

Autologous adipose tissue is used for tissue repletion and/or regeneration as an intact lipoaspirate or as enzymatically derived stromal vascular fraction (SVF), which may be first cultured into mesenchymal stem cells (MSCs). Alternatively, transplant of autologous adipose tissue mechanically fragmented into submillimeter clusters has recently showed remarkable efficacy in diverse therapeutic indications. To document the biologic basis of the regenerative potential of microfragmented adipose tissue, we first analyzed the distribution of perivascular presumptive MSCs in adipose tissue processed with the Lipogems technology, observing a significant enrichment in pericytes, at the expense of adventitial cells, as compared to isogenic enzymatically processed lipoaspirates. The importance of MSCs as trophic and immunomodulatory cells, due to the secretion of specific factors, has been described. Therefore, we investigated protein secretion by cultured adipose tissue clusters or enzymatically derived SVF using secretome arrays. In culture, microfragmented adipose tissue releases many more growth factors and cytokines involved in tissue repair and regeneration, noticeably via angiogenesis, compared to isogenic SVF. Therefore, we suggest that the efficient tissue repair/regeneration observed after transplantation of microfragmented adipose tissue is due to the secretory ability of the intact perivascular niche. Stem Cells Translational Medicine 2018;7:876-886.
Glucose-Coated Gold Nanoparticles Transfer across Human Brain Endothelium and Enter Astrocytes In Vitro (2013)

The blood-brain barrier prevents the entry of many therapeutic agents into the brain. Various nanocarriers have been developed to help agents to cross this barrier, but they all have limitations, with regard to tissue-selectivity and their ability to cross the endothelium. This study investigated the potential for 4 nm coated gold nanoparticles to act as selective carriers across human brain endothelium and subsequently to enter astrocytes. The transfer rate of glucose-coated gold nanoparticles across primary human brain endothelium was at least three times faster than across non-brain endothelia. Movement of these nanoparticles occurred across the apical and basal plasma membranes via the cytosol with relatively little vesicular or paracellular migration; antibiotics that interfere with vesicular transport did not block migration. The transfer rate was also dependent on the surface coating of the nanoparticle and incubation temperature. Using a novel 3-dimensional co-culture system, which includes primary human astrocytes and a brain endothelial cell line hCMEC/D3, we demonstrated that the glucose-coated nanoparticles traverse the endothelium, move through the extracellular matrix and localize in astrocytes. The movement of the nanoparticles through the matrix was >10 µm/hour and they appeared in the nuclei of the astrocytes in considerable numbers. These nanoparticles have the correct properties for efficient and selective carriers of therapeutic agents across the blood-brain barrier.
Analysis of Drug Interaction between Intrathecal Clonidine and MK-801 in Peripheral Neuropathic Pain Rat Model  (1995)

The neuropathic pain is mediated by low-threshold mechanoreceptors, sympathetically dependent, and sensitive to both alpha 2 agonists and N-methyl-D-aspartate antagonists. Intrathecal clonidine may act to diminish sympathetic outflow, whereas MK-801 blocks the N-methyl-D-aspartate receptor that is associated with other spinal systems related to pain transmission mechanism. The two separate mechanisms may account for the powerful synergy observed in this study. Such combinations might be useful in neuropathic pain states to potentiate the antihyperpathic effects and to reduce the side effects of each agents.