gebhart/patrepeval huggingface dataset:
Development of Cytoplasmic-Nuclear Male Sterility, Its Inheritance, and Potential Use in Hybrid Pigeonpea Breeding (2010)

Pigeonpea [Cajanus cajan (L.) Millsp.] is a unique food legume because of its partial (20-30%) outcrossing nature, which provides an opportunity to breed commercial hybrids. To achieve this, it is essential to have a stable male-sterility system. This paper reports the selection of a cytoplasmic-nuclear male-sterility (CMS) system derived from an interspecific cross between a wild relative of pigeonpea (Cajanus sericeus Benth. ex. Bak.) and a cultivar. This male-sterility source was used to breed agronomically superior CMS lines in early (ICPA 2068), medium (ICPA 2032), and late (ICPA 2030) maturity durations. Twenty-three fertility restorers and 30 male-sterility maintainers were selected to develop genetically diverse hybrid combinations. Histological studies revealed that vacuolation of growing tetrads and persistence of tetrad wall were primary causes of the manifestation of male sterility. Genetic studies showed that 2 dominant genes, of which one had inhibitory gene action, controlled fertility restoration in the hybrids. The experimental hybrids such as TK 030003 and TK 030009 in early, ICPH 2307 and TK 030625 in medium, and TK 030861 and TK 030851 in late maturity groups exhibited 30-88% standard heterosis in multilocation trials.
Iron Oxide with Facilitated O<sup>2–</sup> Transport for Facile Fuel Oxidation and CO<sub>2</sub> Capture in a Chemical Looping Scheme (2013)

The chemical looping strategy offers a potentially viable option for efficient carbonaceous fuel conversion with a reduced carbon footprint. In the chemical looping process, an oxygen carrier is reduced and oxidized in a cyclic manner to convert a carbonaceous fuel into separate streams of concentrated carbon dioxide and carbon-free products such as electricity and/or hydrogen. The reactivity and chemical and physical stability of the oxygen carrier are of pivotal importance to chemical looping processes. A typical oxygen carrier is composed of a multi-valence transition metal oxide supported on an “inert” support. Although the support does not get reduced or oxidized at any significant extent, numerous studies have indicated that certain supports such as TiO2 and Al2O3 can improve oxygen carrier stability and/or reactivity. This study reports the use of mixed ionic–electronic conductive support in iron-based oxygen carriers. By incorporating a perovskite-based mixed conductive support such as lanthanum strontium ferrite (LSF), the reactivity of the oxygen carrier is enhanced by 5–70 times when compared to oxygen carriers with conventional TiO2-, Al2O3-, or yttria-stabilized zirconia (YSZ) support. The mixed conductivity enhanced oxygen carrier also shows good stability and coke resistance. Characterization studies indicate that the enhanced oxygen carrier is composed of intermixed nanoscale (<100 nm) crystallites of iron oxide and support. The mixed conductive support enables facile O2– transport to and from the iron oxide nanocrystallites to participate in the surface redox reactions. The support also allows counter-current or concurrent diffusion of electrons or holes to maintain charge balance within the oxygen carrier. With iron oxide as the nanoscale oxygen source and mixed conductive support as the oxygen/electron conductor, the mixed conductivity enhanced oxygen carrier particle can be considered as an ensemble of nanoscale mixed conductive membrane reactors that possess excellent redox activity.
Milk proteins: Physicochemical and functional properties (1984)

Abstract Because of the growing trend toward widespread use of protein ingredients in food formulation and fabrication, an understanding of the relationships between the physical properties of proteins and their behavior in food systems is desirable. A range of milk‐derived protein preparations, i.e., dry milk, milk proteins, caseins, whey proteins, and lactalbumin, are used in a range of food products for their specific functional attributes. In this paper some of the apparent relationships between the properties of the protein components and specific functional properties are discussed. Thus, the roles of milk proteins in determining some important physical characteristics (i.e., color, bulk density, sinkability, dispersibility) of milk powders and their involvement in a range of functional properties (water holding, solubility, Theological behavior, gelation, film formation, emulsification, and foaming) are reviewed. Because of the various methods and conditions used in determining functional properties and the variability in composition of preparations it is difficult to compare data and/or reconcile differences in published information. The desirability of developing standard methods is emphasized.
A new brain‐specific 14‐kDa protein is a phosphoprotein (1993)

We previously reported a new brain-specific protein with a molecular mass of 14 kDa, specifically present in synapses around neurons but not in glial cells [Nakajo, S., Omata, K., Aiuchi, T., Shibayama, T., Okahashi, I., Ochiai, H., Nakai, Y., Nakaya, K. & Nakamura, Y. (1990) J. Neurochem. 55, 2031-2038]. In the present study, we determined the primary structure of this protein, found that it is phosphorylated in vitro and in vivo, and designated it phosphoneuroprotein 14 (PNP 14). The protein is a single polypeptide with 134 amino acid residues (molecular mass = 14122 Da), and it contains a hydrophobic region at the center of the molecule. The carboxy-terminal region has all seven proline residues, and is rich in glutamic acid, which contribute to the acidic property of the protein. The amino-terminal region possesses four unique repetitive motifs, Glu(Ser)-Lys-Thr-Lys-Glu(Gln)-Gly(Gln)-Val(Ala). When a cytosolic fraction prepared from rat cerebral cortex was incubated with [gamma-32P]ATP, 32P was incorporated into PNP 14. Phosphorylated PNP 14 was immunoprecipitated from rat brain synaptosomes labeled metabolically with [32P]orthophosphate. Injection of [32P]orthophosphate into the third ventricle of rat brain resulted in incorporation of radioactive phosphate into PNP 14. We have also found that Ca2+, calmodulin-dependent protein kinase II phosphorylates serine residue(s) of PNP 14 in vitro. The results suggest that PNP 14 may be important to neuronal cells, but not to glial cells, and that its physiological functions may be controlled by the phosphorylation reaction.
Human-centric intelligent systems for exploration and knowledge discovery (2004)

This speculative article discusses research and development relating to computational intelligence (CI) technologies comprising powerful machine-based search and exploration techniques that can generate, extract, process and present high-quality information from complex, poorly understood biotechnology domains. The integration and capture of user experiential knowledge within such CI systems in order to support and stimulate knowledge discovery and increase scientific and technological understanding is of particular interest. The manner in which appropriate user interaction can overcome problems relating to poor problem representation within systems utilising evolutionary computation (EC), machine-learning and software agent technologies is investigated. The objective is the development of user-centric intelligent systems that support an improving knowledge-base founded upon gradual problem re-definition and reformulation. Such an approach can overcome initial lack of understanding and associated uncertainty.
Two different tools for three-dimensional mapping: DE-based scan matching and feature-based loop detection (2013)

SUMMARY An autonomous robot must obtain information about its surroundings to accomplish multiple tasks that are greatly improved when this information is efficiently incorporated into a map. Some examples are navigation, manipulation, localization, etc. This mapping problem has been an important research area in mobile robotics during last decades. It does not have a unique solution and can be divided into multiple sub-problems. Two different aspects of the mobile robot mapping problem are addressed in this work. First, we have developed a Differential Evolution-based scan matching algorithm that operates with high accuracy in three-dimensional environments. The map obtained by an autonomous robot must be consistent after registration. It is basic to detect when the robot is navigating around a previously visited place in order to minimize the accumulated error. This phase, which is called loop detection, is the second aspect studied here. We have developed an algorithm that extracts the most important features from two different three-dimensional laser scans in order to obtain a loop indicator that is used to detect when the robot is visiting a known place. This approach allows the introduction of very different characteristics in the descriptor. First, the surface features include the geometric forms of the scan (lines, planes, and spheres). Second, the numerical features are values that describe several numerical properties of the measurements: volume, average range, curvature, etc. Both algorithms have been tested with real data to demonstrate that these are efficient tools to be used in mapping tasks.
Nonlinear state space modeling and simulation of a SOFC fuel cell (2006)

In order to investigate dynamic behaviors of tubular solid oxide fuel cell (SOFC) and develop a control relevant model for further control studies, a dynamic model based on physical principles is built in the form of nonlinear state-space equation. Dynamics induced by diffusions, inherent impedance, flow, heat exchange and internal reforming/shifting reactions are all considered. Cell temperature, ingredient mole fractions etc. are the state variables and their dynamics are investigated. Dynamic responses of each variable when the external load changes are simulated. Simulation results show that fuel flow, inlet pressure and temperature have significant effects on the dynamics of SOFC. It is further shown that cathode side air inlet temperature has the most direct effect on SOFC performance. Compared with inlet pressures and temperatures, effect of flow velocity is not significant. Simulation also indicates that the transient response of SOFC is mainly controlled by the dynamics of cell temperature owing to its large heat capacity.
Structural Investigations of Vapochromic Behavior. X-ray Single-Crystal and Powder Diffraction Studies of [Pt(CN-<i>iso</i>-C<sub>3</sub>H<sub>7</sub>)<sub>4</sub>][M(CN)<sub>4</sub>] for M = Pt or Pd (1998)

We have synthesized [Pt(CN-iso-C3H7)4][M(CN)4] (M = Pt, Pd) and studied their reversible hydration and sorption properties with UV−vis, FT-IR spectroscopy, and X-ray diffraction. Powder diffraction studies show that anhydrous [Pt(CN-iso-C3H7)4][Pt(CN)4] and [Pt(CN-iso-C3H7)4][Pd(CN)4] crystallize in a tetragonal space group with nearly identical lattice constants. Gravimetric studies reveal that variable guest−host stoichiometries occur when solid [Pt(CN-iso-C3H7)4][Pt(CN)4] sorbs the guest at room temperature from the gas phase [water, 12.1(1) molecules per formula unit, chloroform 6.0(1), methanol 8.0(1), and trifluoroethanol 4.1(1)]; these sorption processes are reversible. The unit cell distances in the tetragonal ab-plane expand dramatically when the solvent guests are sorbed, but changes along the c-axis (the M−M direction) are minimal. Crystallization of [Pt(CN-iso-C3H7)4][Pt(CN)4] from water gives monoclinic crystals of a hexadecahydrate [Pt(CN-iso-C3H7)4][Pt(CN)4]·16H2O. This salt consists of alternating cation/anion chains along b with an average Pt−Pt distance of b/2 = 3.1521(1) Å. The sixteen water molecules per formula weight interlace neighboring chains via H-bonding with each other and the CN- ions of the Pt(CN)42- units. The shifts in the UV−vis and IR spectra that occur when solvent guests are sorbed by the double complex salts are discussed in terms of the lattice expansions that are observed. A mechanism for the lattice expansions that accompany the sorption of guest molecules is proposed.
Glycerol-Based Biorefinery for Fuels and Chemicals (2008)

The rapidly expanding market for bioethanol and biodiesel is remarkably altering the cost and availability of glycerol. In general, approximately 10 pounds of crude glycerol are formed for every 100 pounds of biodiesel produced. Bioethanol process also generates glycerol up to 10% (w/w) of the total sugar consumed as a byproduct. Crude glycerol has thus been widely recognized as an attractive sustainable resource for chemical industries. Glycerol-based biorefinery is the microbial fermentation processes using inexpensive and readily available glycerol as the raw material to produce fuels and chemicals. A major challenge in fermentation of the low-grade crude glycerol is to obtain microbial strains tolerant to undesirable inhibitory components such as salts and organic solvents that present in crude glycerol. There have been several attempts to explore anaerobic microbial assimilation of glycerol using reconstructed microbial systems via microbial screening and metabolic pathway engineering. As a result, fuels as well as some high-value products were found to be produced by microbial fermentation of glycerol. This review describes biological processes using glycerol that produce fuels and chemicals including 1,3-propanediol, ethanol and organic acids.
Metro Ethernet Services - A Technical Overview (2003)

Introduction This white paper provides a comprehensive technical overview of Ethernet services, based on the work (as of April 2003) of the Metro Ethernet Forum (MEF) Technical Committee. The paper is intended to help buyers and users of Ethernet services understand the various types and characteristics of Ethernet services, and to help service providers clearly communicate their service capabilities. Throughout this paper, buyers and users will be collectively referred to as subscribers. This paper will be updated as new work emerges from the MEF Technical Committee.
Enabling “Smart Spaces:” Entity Description and User Interface Generation for a Heterogeneous Component-based Distributed System (1998)

This paper motivates and describes a document-centric framework for component-based distributed systems. In the framework, XML documents are associated with programs that provide either static, immutable interface descriptions as advertisements of functionality at the server-side, or specification of manipulations of these server descriptions to express their usage at clients. We illustrate how the framework allows for 1) remapping of a portion of an existing user interface to a new room control (for example, due to movement of the terminal) 2) viewing of arbitrary subsets and combinations of the functionality available, and 3) mixing dynamically-generated user interfaces with existing user interfaces. <p>The use of a document-centric framework in addition to a conventional object-oriented programming language provides a number of key features. One of the most useful is that it exposes program/UI to referent objects mappings, thereby providing a standard location for manipulation of this indirection
Microstrip circuit analog of a complex diffraction phenomenon (2009)

This letter presents a methodology to reproduce at microwave frequencies the physical behavior of certain diffraction structures that have been investigated in the optics community. The methodology will be exemplified with a microstrip circuit implementation of a transmission line system exhibiting an electromagnetic response that mimics the response of simple and compound diffraction gratings. The identification of such “bridge” circuit analogies could be very fruitful for the understanding and development of design strategies to devise practical components based on phenomena employed in the affine areas of optics and microwave engineering.
ATM-depletion in breast cancer cells confers sensitivity to PARP inhibition (2013)

These data indicate that ATM-depletion can sensitize breast cancer cells to PARP inhibition, suggesting a potential in the treatment of breast cancers low in ATM protein expression/activity, such as those arising in mutant ATM heterozygous carriers.
LL-37 Complexation with Glycosaminoglycans in Cystic Fibrosis Lungs Inhibits Antimicrobial Activity, Which Can Be Restored by Hypertonic Saline (2009)

There is an abundance of antimicrobial peptides in cystic fibrosis (CF) lungs. Despite this, individuals with CF are susceptible to microbial colonization and infection. In this study, we investigated the antimicrobial response within the CF lung, focusing on the human cathelicidin LL-37. We demonstrate the presence of the LL-37 precursor, human cathelicidin precursor protein designated 18-kDa cationic antimicrobial protein, in the CF lung along with evidence that it is processed to active LL-37 by proteinase-3. We demonstrate that despite supranormal levels of LL-37, the lung fluid from CF patients exhibits no demonstrable antimicrobial activity. Furthermore Pseudomonas killing by physiological concentrations of exogenous LL-37 is inhibited by CF bronchoalveolar lavage (BAL) fluid due to proteolytic degradation of LL-37 by neutrophil elastase and cathepsin D. The endogenous LL-37 in CF BAL fluid is protected from this proteolysis by interactions with glycosaminoglycans, but while this protects LL-37 from proteolysis it results in inactivation of LL-37 antimicrobial activity. By digesting glycosaminoglycans in CF BAL fluid, endogenous LL-37 is liberated and the antimicrobial properties of CF BAL fluid restored. High sodium concentrations also liberate LL-37 in CF BAL fluid in vitro. This is also seen in vivo in CF sputum where LL-37 is complexed to glycosaminoglycans but is liberated following nebulized hypertonic saline resulting in increased antimicrobial effect. These data suggest glycosaminoglycan-LL-37 complexes to be potential therapeutic targets. Factors that disrupt glycosaminoglycan-LL-37 aggregates promote the antimicrobial effects of LL-37 with the caveat that concomitant administration of antiproteases may be needed to protect the now liberated LL-37 from proteolytic cleavage.
Carbon Dioxide as a Protecting Group: Highly Efficient and Selective Catalytic Access to Cyclic <i>cis</i>‐Diol Scaffolds (2014)

The efficient and highly selective formation of a wide range of (hetero)cyclic cis-diol scaffolds using aminotriphenolate-based metal catalysts is reported. The key intermediates are cyclic carbonates, which are obtained in high yield and with high levels of diastereo- and chemoselectivity from the parent oxirane precursors and carbon dioxide. Deprotection of the carbonate structures affords synthetically useful cis-diol scaffolds with different ring sizes that incorporate various functional groups. This atom-efficient method allows the simple construction of diol synthons using inexpensive and accessible precursors and green metal catalysts and showcases the use of CO2 as a temporary protecting group.
Noise in AlGaAs/InGaAs/GaAs pseudomorphic HEMTs from 10 Hz to 18 GHz (1993)

Noise properties of AlGaAs/InGaAs/GaAs pseudomorphic HEMTs (PHEMTs) have been investigated simultaneously in the low and intermediate frequency range (10 Hz to 150 MHz) and in the microwave range (4 to 18 GHz) and compared to the noise of more classical devices such as MESFETs and GaAlAs/GaAs HEMTs. Unlike the other commercially available devices, PHEMTs exhibit the unique capability of providing simultaneously state-of-the-art microwave noise performance and a reasonable low-frequency excess noise.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Inverse correlation between IL‐7 receptor expression and CD8 T cell exhaustion during persistent antigen stimulation (2005)

Persistence is a hallmark of infection by viruses such as HIV, hepatitis B virus, hepatitis C virus and LCMV. In the case of LCMV, persistence may often be associated with exhaustion of CD8(+) T cells. We demonstrate here that persistent antigen suppressed IL-7Ralpha expression and this correlated with T cell exhaustion and reduced expression of the anti-apoptotic molecule B cell leukemia/lymphoma 2 (Bcl-2). In contrast, exposure to short-lived antigen only temporarily suppressed IL-7Ralpha expression, failed to induce T cell exhaustion, and primed T cells. Persistent antigen also suppressed IL-7Ralpha expression on primed T cells and this correlated with exhaustion of a previously stable primed T cell population. These findings suggest that antigen longevity regulates T cell fate.
Automated mixed-signal SoC BIST synthesis utilizing hardware accelerators (2012)

BIST techniques for analog and mixed-signal circuits have attracted considerable research activity; especially utilizing on-chip ROMs to store high precision sinusoidal stimuli and pre-calculated Delta-Sigma modulated bit-streams. However, usage of ROMs in high-performance circuits has poses substantial challenges, mainly because of its inability to run at-speed tests and high area overhead due to its prohibitively large size. An alternative to ROM utilization is the use of LFSRs. But, the computation time of the LFSR based BIST synthesis for large mixed-signal SoC poses a huge challenge. A high-performance computing (HPC) based automated mixed-signal SoC BIST synthesis technique that can outperform the conventional ROM implementation not only with respect to the computation time needed to generate the test vectors or waveforms, but also the BIST hardware required, is presented in this paper. Furthermore, the versatility of the presented LFSR based BIST test vector generator, that allows itself to be used for embedding deterministic patterns for LBIST and storing sinusoidal stimuli or pre-calculated Delta-Sigma modulated bit-stream for analog BIST, is demonstrated.
Analysis and Preparation of Bartonella Bacilliformis Antigens (1988)

Twenty-four antigens of Bartonella bacilliformis, a bacterium which causes bartonellosis in residents of high altitude valleys of the Andes, were identified by immunoblot and immunoprecipitation using rabbit anti-Bartonella sera as well as sera of patients. The antigens were designated according to their relative molecular mass which ranged from 16 to 160 kDa. Twelve antigens were detected by antibodies in sera of bartonellosis patients using immunoblot, of which six antigens were detected by immunoprecipitation. Antigens 25, 46, 65, 75, 99, and 160 were identified as probable cell wall antigens. Antigens 50, 65, and 75 detected long-persisting antibodies. Crude Bartonella antigen applied to ELISA reacted with anti-Chlamydia psittaci antibody as well as with antibody of unknown identity in human sera, whereas immunoblot and immunoprecipitation with Triton soluble antigens revealed Bartonella-specific results. Seven Bartonella antigens were prepared by high performance liquid chromatography of which one antigen (48 kDa) reacted Bartonella-specific when applied to ELISA. It was concluded that specificity of antibody determination with crude Bartonella antigen should be confirmed by either immunoblot or immunoprecipitation.
Green Chemical Engineering Aspects of Reactive Distillation (2003)

Reactive or catalytic distillation technology combines chemical synthesis steps with separations by distillation. This combination can lead to intensified, high-efficiency process systems with significant green engineering attributes. New applications and understanding have prompted growth in the use of reactive distillation for a variety of chemical syntheses, especially esterifications and etherifications involving oxygenated hydrocarbons. We describe several applications and the potential and tradeoffs for reactive distillation technology in the context of green engineering principles.