gebhart/patrepeval huggingface dataset:
Macrophage migration inhibitory factor (MIF) regulates host responses to endotoxin through modulation of Toll-like receptor 4 (TLR4) (2003)

The cytokine macrophage migration inhibitory factor (MIF) has emerged recently as an important mediator of inflammation and innate immunity. MIF is rapidly released by macrophages after stimulation with microbial products and pro-inflammatory cytokines and, in turn, stimulates the production of pro-inflammatory mediators by immune cells. Immunoneutralization of MIF or deletion of the Mif gene was shown to protect animals from lethal endotoxemia, staphylococcal toxic shock and septic shock in experimental models of bacterial peritonitis. To investigate the function of MIF in innate immunity, we studied the response of macrophages expressing reduced levels of MIF to microbial products. These cells were generated by transduction of an antisense MIF adenovirus or by stable transfection with an antisense MIF plasmid or were obtained from MIFknockout mice. MIF-deficient macrophages were shown to be hyporesponsive to stimulation with LPS and Gram-negative bacteria. The defect was associated with a down-regulation of Toll-like receptor 4 (TLR4), the signal transducing molecule of the LPS receptor complex. Immunoneutralization of extracellular MIF decreased TLR4 expression and responses of macrophages to LPS, indicating that MIF may exert autocrine effects. These findings identify an important role for MIF in innate immunity and provide a rationale for the development of anti-MIF strategy for the treatment of patients with Gram-negative septic shock.
In search of a super oral rehydration solution: can optimum use of organic solute-mediated sodium absorption lead to the development of an absorption promoting drug? (1983)

A hypothesis is proposed that may lead to the development of a oral rehydration solution (ORS). Available literature was critically reviewed. Suggested were possible ways of further enhancing the absorptions of sodium and water from the small intestine in acute diarrhea through water soluble organic solute-linked transport without imposing an osmotic penalty due to unabsorbed organic compounds. These ways are: use of suitable polymers such as polysaccharides and proteins; use of mixtures of rapidly absorbed organic molecules such as d-hexoses and amino acids; and use of complex and variable mixtures of polysaccharides proteins protein hydrolysates oligo- and monosaccharides di- and tripeptides and amino acids. Based on these principles optimum concentrations of organic compounds on ORS and use of compounds other than glucose (including sucrose glucose polymer and rice powder) are discussed. Through clinical trials it already has been shown that addition of the amino acid glycine to standard glucose-containing ORS enhanced absorption of sodium and water without imposing an osmotic penalty. The explore the possibility of further enhancing the absorption of ORS clinical trials were conducted in infants and children who had moderate-to-severe dehydration due to diarrhea caused by rotavirus Vibrio cholerae enterotoxigenic Escherichia coli and others. The control group received standard WHO recommended ORS with glucose while 1 study group received the same solution with added glycine and the other received ORS in which 20 g glucose was replaced by 50 g of popped rice powder for each liter of fluid. Compared to the controls in the groups treated with rice ORS and glycine-glucose ORS respectively there was a significant reduction of 3 things: diarrheal stool output by 49% and by 50%; mean duration of diarrhea by 30% in each group; and volume of ORS needed to treat them by 36% and 43% respectively. In addition to replacing diarrheal losses these 2 study solutions led to reduced magnitude and duration of diarrhea. Thus the 2 solutions assumed the role of highly effective absorption-promoting drugs. The results were superior to the effects of antisecretory drugs so far being tested in human studies. The future prospect of the hypothesis is discussed. A series of in vivo animal and human studies is in progress to define the optimum composition of a super ORS. (authors modified)
Recycling of polyethylene terephthalate (PET) plastic bottle wastes in bituminous asphaltic concrete (2016)

This research sheds light on the concept of eco-friendly road construction which comprises eco-design, eco-extraction, eco-manufacturing, eco-construction,\neco-rehabilitation, eco-maintenance, eco-demolition, and socioeconomic empowerment. It also revealed the challenges being faced in its adoption and the benefits derivable from its application. Furthermore, the effects of recycling PET plastic bottle wastes produced in North Central Nigeria in bituminous asphaltic concrete (BAC) used in flexible pavement construction were also evaluated. The mix design consists of 60/70 penetration-grade asphaltic concrete (5%), 68% coarse aggregate, 6% fine aggregate, and 21% filler using the dry process at 170°C. The optimum bitumen\ncontent (OBC) for conventional BAC was obtained as 4% by weight of total aggregates and filler. Polymer-coated aggregate (PCA)-modified BAC seems preferable because it has the potential to utilize more plastic wastes with a higher optimum plastic content (OPC) of 16.7% by weight of total aggregates and filler compared to that of 9% by weight of OBC achieved by PMB-BAC. For both PMB- and PCA-modified BAC, an increase in air void, void in mineral aggregate, and Marshall stability were observed. Eco-friendly road construction which recycles PET wastes should be encouraged by government considering its potential environmental and economic\nbenefits.
Identification and characterization of TMEM16H gene in silico (2005)

TMEM16A gene is located within the CCND1-ORAOV1-FGF19-FGF4-FGF3-TMEM16A-FADD-PPFIA1-CTTN (cortactin or EMS1) amplicon at human chromosome 11q13.3. TMEM16A, TMEM16B, TMEM16C, TMEM16D, TMEM16E (GDD1), TMEM16F, TMEM16G and TP53I5 are TMEM16 family proteins with TM16H1, TM16H2 and TM16H3 domains. Here, we identified and characterized TMEM16H gene by using bioinformatics. Human TMEM16H gene, consisting of 18 exons, was located at human chromosome 19p13.11. AB046843.2 cDNA corresponded to TMEM16H isoform 1 without intron 17, while CR616031.1 cDNA corresponded to TMEM16H isoform 2 with retained intron 17. TMEM16H isoforms were eight-transmembrane proteins with TM16H3 domain. The last exon of isoforms 1 and 2 corresponded to C-terminal 122 and 227 aa, respectively. Human TMEM16H isoform 1 was the major transcript, while isoform 2 was the minor transcript prone to non-sense mediated mRNA decay (NMD). TMEM16H mRNA was expressed in embryonic stem (ES) cells, fetal brain, and neural tissues. Mouse Tmem16h representative cDNA was determined by assembling BC027735.1 and BC059855.1 cDNAs. Mouse and rat Tmem16h genes encoded Tmem16h proteins similar to human TMEM16H isoform 2. The regions corresponding to exons 17 and 18 of human TMEM16H gene were not separated by intron within mouse and rat Tmem16h genes. Insertion of intron 17 into human TMEM16H gene during evolution resulted in the generation of TMEM16H isoform 1. This is the first report on identification and characterization of the TMEM16H gene as well as on the molecular evolution of TMEM16H.
Regulation of transcription in vitro from herpes simplex virus genes (1986)

In vitro transcription assays were carried out by using as templates DNAs cut from the herpes simplex virus early glycoprotein D gene, the late glycoprotein C gene, the late VP5 gene, and the immediate-early ICP22 gene. Nuclear extracts from suspension cultures of uninfected HeLa cells effectively synthesized RNAs from genes of the immediate-early and delayed-early classes. To a lesser extent, the extracts also used DNAs cut from the late genes as templates. Transcription from the immediate-early gene was inhibited in extracts prepared from infected cells. Analysis of the proteins in infected-cell extracts by gel electrophoresis, transfer to nitrocellulose, and probing with specific antibody demonstrated the presence of the viral regulatory protein ICP4. Chromatographic fractionation of nuclear extract from infected cells yielded a mixture of proteins (fraction VIII) enriched in ICP4 (S.W. Faber and K.W. Wilcox, Nucleic Acids Res., 14:6067-6083, 1986). Addition of fraction VIII to the in vitro assay affected transcription. Depending on the DNA in the assay, an inhibitory or stimulatory effect was observed. Inhibition of RNA synthesis was found when DNA from the immediate-early gene was used as a template, and stimulation was found when DNA from the early or late gene was used.
Epigallocatechin-3-gallate (EGCG), A Green Tea Polyphenol, Suppresses Hepatic Gluconeogenesis through 5′-AMP-activated Protein Kinase (2007)

Epigallocatechin-3-gallate (EGCG), a main catechin of green tea, has been suggested to inhibit hepatic gluconeogenesis. However, the exact role and related mechanism have not been established. In this study, we examined the role of EGCG in hepatic gluconeogenesis at concentrations that are reachable by ingestion of pure EGCG or green tea, and are not toxic to hepatocytes. Our results show in isolated hepatocytes that EGCG at relatively low concentrations (<or=1 microm) inhibited glucose production via gluconeogenesis and expression of key gluconeogenic genes. EGCG was not toxic at these concentrations while demonstrating significant cytotoxicity at 10 mum and higher concentrations. EGCG at 1 mum or lower concentrations effective in suppressing hepatic gluconeogenesis did not activate the insulin signaling pathway, but activated 5'-AMP-activated protein kinase (AMPK). The EGCG suppression of hepatic gluconeogenesis was prevented by blockade of AMPK activity. In defining the mechanism by which EGCG activates AMPK, we found that the EGCG activation of AMPK was mediated by the Ca(2+)/calmodulin-dependent protein kinase kinase (CaMKK). Furthermore, our results show that the EGCG activation of AMPK and EGCG suppression of hepatic gluconeogenesis were both dependent on production of reactive oxygen species (ROS), which was a known activator of CaMKK. Together, our results demonstrate an inhibitory role for EGCG in hepatic gluconeogenesis and shed new light on the mechanism by which EGCG suppresses gluconeogenesis.
A Standardized Approach to Pediatric Parenteral Medication Delivery (2004)

Delivering medications safely to hospitalized pediatric patients presents a significant challenge. Current JCAHO standards require safe medication practices, including the standardization of parenteral medications and solutions. Our institution convened a pediatric process improvement team (consisting of pharmacists, nurses, physicians, and administrators) to address deficiencies in our medication use process. This article describes the standardized parenteral medication processes that this committee ultimately endorsed. The new system was designed to control, simplify, and standardize each major step in the medication use process, from prescribing through administration. The system involved defining and standardizing prescribing practices, which then allowed for reduced variability and the preplanning of drug preparation and administration processes. To ease transition and simplify implementation, three phases of the process were introduced in a step-by-step manner: phase 1, continuous infusions; phase 2, intermittent IV doses; and phase 3, bulk IV fluids. Time to implementation included 6 months of preparation prior to phases 1 and 2 and approximately another year to phase 3. The standardized pediatric parenteral medication process has resulted in improved patient safety and operational efficiency. It has also eliminated the “Rule of six” method of drug preparation and administration.
Monobloc and Facial Bipartition Distraction with Internal Devices (1999)

Distraction osteogenesis (DO) permits gradual lengthening of the craniofacial skeleton. With the advent of new internal devices, monobloc (M) and facial bipartition (FB) DO are feasible. The rationale behind M and FB distraction is (1) gradual advancement of the M segment is not associated with a substantial retrofrontal dead space; (2) because 5 to 7 days elapse prior to distraction, the nasofrontal opening, in theory, is allowed to remucosalize; (3) gradual expansion of the soft tissues takes advantage of skin creep, potentially limiting relapse; (4) the procedure appears to be less invasive with decreased blood loss and operative time, enabling its use in infants; (5) overdistraction may eliminate or reduce the frequency of subsequent procedures; and (6) the procedure may be combined with FB and skull vault remodeling to provide excellent results in more complex craniofacial dysostosis problems. Five children underwent M advancement (N = 3) and M with FB (N = 2) at 9 months to 5 years of age to correct functional abnormalities such as corneal exposure, increased intracranial pressure, and apnea, as well as severe craniofacial disfigurement. Each patient underwent from 22 to 30 mm of distraction with the Modular Internal Distraction (MID) system, developed by the first author (SRC). There was one infection late in the series along the DO cable track. There were no cases of epidural abscess. In conclusion, MDO, with and without FB, appears to be a safe and effective technique for transcranial frontofacial advancement. The morbidity of the procedure appears to be less than that of conventional M advancement.
An Effective Mixing for Lithium Ion Battery Slurries (2014)

Coating slurries for making anodes and cathodes of lithium batteries contain a large percentage of solid particles of different chemicals, sizes and shapes in highly viscous media. A thorough mixing of these slurries poses a major challenge in the battery manufacturing process. Several types of mixing devices and mixing methods were examined. The conventional turbine stirrers or ball mill mixers could be adequately used for the preparation of anode slurries, but not suitable for cathode slurries. In this study, a newly three-dimensional mixer, in conjunction with a multi-stage mixing sequence was proposed. The mixing effectiveness was examined by means of rheological measurements and flow visualization techniques. Preliminary electrical performance results indicated that the battery obtained using the 3D mixing device with a multi-stage mixing sequence was more efficient to those obtained from conventional methods.
Lectins Identify Glycan Biomarkers on Glioblastoma-Derived Cancer Stem Cells (2012)

Glioblastoma (GBM) is a highly aggressive primary brain tumor with a poor prognosis. Despite aggressive therapy with surgery, radiotherapy, and chemotherapy, nearly all patients succumb to disease within 2 years. Several studies have supported the presence of stem-like cells in brain tumor cultures that are CD133-positive, are capable of self-renewal, and give rise to all cell types found within the tumor, potentially perpetuating growth. CD133 is a widely accepted marker for glioma-derived cancer stem cells; however, its reliability has been questioned, creating a need for other identifiers of this biologically important subpopulation. We used a panel of 20 lectins to identify differences in glycan expression found in the glycocalyx of undifferentiated glioma-derived stem cells and differentiated cells that arise from them. Fluorescently labeled lectins that specifically recognize α-N-acetylgalactosamine (GalNAc) and α-N-acetylglucosamine (GlcNAc) differentially bound to the cell surface based on the state of cellular differentiation. GalNAc and GlcNAc were highly expressed on the surface of undifferentiated cells and showed markedly reduced expression over a 12-day duration of differentiation. Additionally, the GalNAc-recognizing lectin Dolichos biflorus agglutinin was capable of specifically selecting and sorting glioma-derived stem cell populations from an unsorted tumor stock and this subpopulation had proliferative properties similar to CD133(+) cells in vitro and also had tumor-forming capability in vivo. Our preliminary results on a single cerebellar GBM suggest that GalNAc and GlcNAc are novel biomarkers for identifying glioma-derived stem cells and can be used to isolate cancer stem cells from unsorted cell populations, thereby creating new cell lines for research or clinical testing.
Enhanced and Stable Field Emission from in Situ Nitrogen-Doped Few-Layered Graphene Nanoflakes (2011)

Vertically aligned few-layered graphene (FLG) nanoflakes were synthesized on bare silicon (Si) substrates by a microwave plasma enhanced chemical vapor deposition method. In situ nitrogen (N2) plasma treatment was carried out using electron cyclotron resonance plasma, resulting in various nitrogen functionalities being grafted to the FLG surface. Compared with pristine FLGs, the N2 plasma-treated FLGs showed significant improvement in field emission characteristics by lowering the turn-on field (defined at 10 μA/cm2) from 1.94 to 1.0 V/μm. Accordingly, the field emission current increased from 17 μA/cm2 at 2.16 V/μm for pristine FLGs to about 103 μA/cm2 at 1.45 V/μm for N-doped FLGs. Furthermore, N-doped FLG samples retained 94% of the starting current over a period of 10 000 s, during which the fluctuations were of the order of ±10.7% only. The field emission behavior of pristine and N2 plasma-treated FLGs is explained in terms of change in the effective microstructure as well as a reduction in the work function as probed by X-ray photoelectron valence band spectroscopy.
Pancreatic arteriovenous malformation treated by transcatheter embolization: report of a case with hepatocellular carcinoma. (1991)

Arteriovenous malformations (AVMs) of the pancreas are rare and frequently complicated by gastrointestinal bleeding. We present a case of AVM of the pancreas accompanied by arterioportal shunt (AP shunt) in a patient with hepatocellular carcinoma and cirrhosis. Therapeutic embolization of AVM was successfully carried out. The clinical significance of this intervention is also discussed.
Comparative studies on anti-inflammatory activity of Coriandrum sativum, Datura stramonium and Azadirachta indica. (2010)

Coriandrum sativum, Datura stramonium and Azadirachta indica are traditionally used in treatment of inflammation. Ethanolic extracts of fruits of Coriandrum sativum, leaves of Datura stramonium and Azadirachta indica were subjected to preliminary screening for anti-inflammatory activity in albino rats. All ethanolic extracts exhibited significant anti-inflammatory activity comparable to the standard drug Diclofenac sodium against carrageenan induced rat paw edema method. Among these plant Azadirachta indica showed maximum anti-inflammatory activity every hour.
Direct Visualization of Cytokine-Producing Recall Antigen-Specific CD4 Memory T Cells in Healthy Individuals and HIV Patients (2000)

We have used computer-assisted cytokine ELISA spot analysis to measure the frequencies, the type of cytokine, and the amount of cytokine produced by individual recall Ag-specific CD4 memory cells in freshly isolated blood. We studied the memory cells specific for tetanus toxoid and purified protein derivative in 18 healthy individuals and in 22 HIV-infected patients on highly active antiretroviral therapy (HAART). In healthy individuals, the frequency, cytokine signature, and cytokine production per cell of these memory cells were stable over time. Although it is presently unclear whether the maintenance of the memory T cell pool depends upon Ag persistence, cross-reactive Ag stimulation, or cytokine-driven bystander stimulations and expansions, our data strongly argue for a stable memory cell pool in healthy individuals. In HIV patients, however, the frequency of these memory cells was a function of the viral load. The decreased numbers of functional memory cells in patients with high viral loads might provide one mechanism behind the immunodeficient state. Although the cytokine output per cell was unaffected in most patients (20 of 24), in some patients (4 of 24) it was >100-fold reduced, which might provide an additional mechanism to account for the reduced immunocompetence of these patients. The ability to visualize directly and quantify the cytokine produced by the low frequency memory cells in freshly isolated blood that have been physiologically stimulated by Ag should aid comprehensive studies of the Ag-specific memory cell pool in vivo, in health and disease.
Bounds, Inequalities, and Monotonicity Properties for Some Specialized Renewal Processes (1980)

Renewal processes with increasing mean residual life and decreasing failure rate interarrival time distributions are investigated. Various two-sided bounds are obtained for $M(t)$, the expected number of renewals in $\lbrack 0, t\rbrack$. It is shown that if the interarrival time distribution has increasing mean residual life with mean $\mu$, then the expected forward recurrence time is increasing in $t \geqslant 0$, as is $M(t) - t/\mu$. If the interarrival time distribution has decreasing failure rate then $M(t)$ is concave, and the forward and backward recurrence time distributions are stochastically increasing in $t \geqslant 0$.
Analysis of FOXP3 protein expression in human CD4+CD25+ regulatory T cells at the single-cell level (2005)

The transcription factor FOXP3 plays a key role in CD4(+)CD25(+) regulatory T cell function and represents a specific marker for these cells. Despite its strong association with regulatory T cell function, in humans little is known about the frequency of CD4(+)CD25(+) cells that express FOXP3 protein nor the distribution of these cells in vivo. Here we report the characterization of seven anti-FOXP3 monoclonal antibodies enabling the detection of endogenous human FOXP3 protein by flow cytometry and immunohistochemistry. Flow-cytometric analysis showed that FOXP3 was expressed by the majority of CD4(+)CD25(high) T cells in peripheral blood. By contrast, less than half of the CD4(+)CD25(int) population were FOXP3(+), providing an explanation for observations in human T cells that regulatory activity is enriched within the CD4(+)CD25(high) pool. Although FOXP3 expression was primarily restricted to CD4(+)CD25(+) cells, it was induced following activation of both CD4(+) and CD8(+) T cell clones. These findings indicate that the frequency of FOXP3(+) cells correlates with the level of expression of CD25 in naturally arising regulatory T cells and that FOXP3 protein is expressed by some activated CD4(+) and CD8(+) T cell clones. These reagents represent valuable research tools to further investigate FOXP3 function and are applicable for routine clinical use.
Rules for ring closure: ring formation by conjugate addition of oxygen nucleophiles (1977)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTRules for ring closure: ring formation by conjugate addition of oxygen nucleophilesJack E. Baldwin, Richard C. Thomas, Lawrence I. Kruse, and Lee SilbermanCite this: J. Org. Chem. 1977, 42, 24, 3846–3852Publication Date (Print):November 1, 1977Publication History Published online1 May 2002Published inissue 1 November 1977https://pubs.acs.org/doi/10.1021/jo00444a011https://doi.org/10.1021/jo00444a011research-articleACS PublicationsRequest reuse permissionsArticle Views4133Altmetric-Citations325LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts