gebhart/patrepeval huggingface dataset:
Comparison of PCR-Based Methods for Typing <i>Staphylococcus aureus</i> Isolates (2006)

In this study, we compared the potentials of (i) a multiplex PCR-based multilocus variable-number tandem repeat (VNTR) assay; (ii) a triplex PCR coamplifying fragments of spa, coa, and the hypervariable region adjacent to the mecA gene; (iii) restriction profile analysis of the STAR repetitive element; (iv) randomly amplified polymorphic DNA analysis; (v) inter-IS256 PCR; and (vi) rep-MP3 PCR. Multilocus VNTR typing and triplex PCR (coa, spa, and hypervariable region) approaches showed excellent reproducibility and high discriminatory power; however, only multilocus VNTR typing could distinguish all pulsed-field gel electrophoresis and spa types. Multilocus VNTR typing appears to be the most useful PCR-based method for the rapid genotyping of Staphylococcus aureus strains.
DIMENSÕES E FUNÇÕES DO CONHECIMENTO PEDAGÓGICO-MUSICAL (2000)

O artigo discute o campo epistemologico da educacao musical abordando questoes sobre o objetivo de estudo da area, a natureza do conhecimento pedagogico- musical e suas inter-relacoes com outras areas do conhecimento. Nesta abordagem o texto procura revelar as funcoes e as particularidades do conhecimento pedagogico-musical em suas dimensoes filosoficas, antropologicas, pedagogicas, sociologicas, historicas, esteticas, psicologicas e musicologicas. Apresenta-se um modelo estrutural da concepcao e da acao pedagogico-musical que mostra a sua diferente perspectiva do conhecimento e com isso o entrelacamento com outras disciplinas cientificas, tornando transparente a associacao da pratica social e estetica, bem como as tarefas da producao do conhecimento.
Fast algorithms for approximate semidefinite programming using the multiplicative weights update method (2005)

Semidefinite programming (SDP) relaxations appear in many recent approximation algorithms but the only general technique for solving such SDP relaxations is via interior point methods. We use a Lagrangian-relaxation based technique (modified from the papers of Plotkin, Shmoys, and Tardos (PST), and Klein and Lu) to derive faster algorithms for approximately solving several families of SDP relaxations. The algorithms are based upon some improvements to the PST ideas - which lead to new results even for their framework - as well as improvements in approximate eigenvalue computations by using random sampling.
Normalization of CD4 <sup>+</sup> T cell metabolism reverses lupus (2015)

Systemic lupus erythematosus (SLE) is an autoimmune disease in which autoreactive CD4(+) T cells play an essential role. CD4(+) T cells rely on glycolysis for inflammatory effector functions, but recent studies have shown that mitochondrial metabolism supports their chronic activation. How these processes contribute to lupus is unclear. We show that both glycolysis and mitochondrial oxidative metabolism are elevated in CD4(+) T cells from lupus-prone B6.Sle1.Sle2.Sle3 (TC) mice as compared to non-autoimmune controls. In vitro, both the mitochondrial metabolism inhibitor metformin and the glucose metabolism inhibitor 2-deoxy-d-glucose (2DG) reduced interferon-γ (IFN-γ) production, although at different stages of activation. Metformin also restored the defective interleukin-2 (IL-2) production by TC CD4(+) T cells. In vivo, treatment of TC mice and other lupus models with a combination of metformin and 2DG normalized T cell metabolism and reversed disease biomarkers. Further, CD4(+) T cells from SLE patients also exhibited enhanced glycolysis and mitochondrial metabolism that correlated with their activation status, and their excessive IFN-γ production was significantly reduced by metformin in vitro. These results suggest that normalization of T cell metabolism through the dual inhibition of glycolysis and mitochondrial metabolism is a promising therapeutic venue for SLE.
UTILIZATION OF SOME FRUITS AND VEGETABLES WASTES AS A SOURCE OF DIETARY FIBERS IN CAKE MAKING (2013)

In this study, feasibility of using orange waste (OW), carrot pomace (CP), potato peels (PP) and green pea peels (GPP) wastes from food industry, as a starting raw material to produce dietary fibre powders and the feasibility of producing cakes intended for people suffering from obesity or over weight and diabetes. The physicochemical properties of dietary fiber powder were first evaluated. The results showed that the fibre contents, compositions and hydration properties (water and oil holding capacity and swelling capacity) of the fiber powder. Some food processing wastes {(OW), (CP), (PP) and (GPP)}, are a rich sources of fibres. The OW, CP, PP and GPP wastes were replaced with wheat flour (72%) at 5, 10, 15 and 20% levels and studied for rheological characteristics. Water absorption increased significantly with increasing wastes from 0 to 20%. Dough stability and dough development were increased. Resistance to extension values significantly increased, whereas extensibility values decreased. The produced fiber substituted cakes achieved a reduction in calories. Cakes were prepared from blends of wheat flour (72%) with 5, 10, 15 and 20% fruits and vegetables wastes. The volume of cakes decreased with increase in fruits and vegetables wastes content from 0 to 20%. Cakes prepared from 20% of wastes OW, CP, PP and GPP had a higher ratio of dietary fibres. Sensory evaluation showed that all high fiber substituted cake samples were significantly lower than control cake sample in all sensory characteristics, except cake samples prepared with 5 and 10% of orange waste and carrot pomace had no significant differences (P>0.05) with control cake. However, there were significant differences between cake samples containing the same type of fiber source at 5, 10, 15 and 20% replacement levels. The highest and lowest scores in the same type of fiber source for all attributes were that achieved by cake samples with fiber source at 5 and 20% replacement levels, respectively. Texture properties of cake were affected by replacement levels of fiber source. The results indicated that OW, CP, PP and GPP wastes can serve as a good source of dietary fibres.
Data Temperature-Aware Bloom Filters for Flash-Based Storage (2019)

Hot data identification techniques for flash memory have attracted a plethora of research interests because of its crucial impact on performance and life span of SSD. Our proposed technique, Data Temperature-Aware Bloom Filters (DTABF), is suitable for the requirements of both the Buffer Management and the Flash Translation Layer in SSD. DTABF divides the access period of LPAs into n cycles. Thus, DTABF can record the access frequency of LPAs with a round-robin fashion in each cycle by combining one Counting Bloom filter and n Bloom filters. The access pattern of each LPA, indicated by the data access frequency, recency, and access frequency changes over time, is presented with n different data temperatures in n cycles. Specifically, we employ a bijective function to characterize the data temperature of each LPA. Based on the data temperature identified by DTABF, the data stored in LPAs, which presenting the similar access pattern, can be gathered in the same flash block in SSD. Meanwhile, those data occasionally becoming cold in the buffer can be effectively identified to avoid being meaninglessly replaced from the buffer. Analytical and experimental results show that DTABF can help to improve hit rate, write performance and alleviate write amplification, while achieving lower memory cost and computational complexity.
Flexible and Highly Scalable V<sub>2</sub>O<sub>5</sub>‐rGO Electrodes in an Organic Electrolyte for Supercapacitor Devices (2014)

Vanadium pentoxide–reduced graphene oxide (rGO) free‐standing electrodes are used as electrodes for supercapacitor applications, eliminating the need for current collectors or additives and reducing resistance (sheet resistance 29.1 Ω □ −1 ). The effective exfoliation of rGO allows improved electrolyte ions interaction, achieving high areal capacitance (511.7 mF cm −2 ) coupled with high mass loadings. A fabricated asymmetric flexible device based on rGO/V 2 O 5 ‐rGO (VGO) consists of approximately 20 mg of active mass and still delivers a low equivalent series resistance (ESR) of 3.36 Ω with excellent cycling stability. A prototype unit of the assembled device with organic electrolyte is shown to light up eight commercial light‐emitting diode bulbs.
VEGF may contribute to macrophage recruitment and M2 polarization in the decidua (2018)

It is increasingly evident that cytokines and growth factors produced in the decidua play a pivotal role in the regulation of the local immune microenvironment and the establishment of pregnancy. One of the major growth factors produced in the decidua is vascular endothelial growth factor (VEGF), which acts not only on endothelial cells, but also on multiple other cell types, including macrophages. We sought to determine whether decidua-derived VEGF affects macrophage recruitment and polarization using human endometrial/decidual tissue samples, primary human endometrial stromal cells (ESCs), and the human monocyte cell line THP1. In situ hybridization was used for assessment of local VEGF expression and immunohistochemistry was used for identification and localization of CD68-positive endometrial macrophages. Macrophage migration in culture was assessed using a transwell migration assay, and the various M1/M2 phenotypic markers and VEGF expression were assessed using quantitative real-time PCR (qRT-PCR). We found dramatic increases in both VEGF levels and macrophage numbers in the decidua during early pregnancy compared to the secretory phase endometrium (non-pregnant), with a significant increase in M2 macrophage markers, suggesting that M2 is the predominant macrophage phenotype in the decidua. However, decidual samples from preeclamptic pregnancies showed a significant shift in macrophage phenotype markers, with upregulation of M1 and downregulation of M2 markers. In THP1 cultures, VEGF treatment significantly enhanced macrophage migration and induced M1 macrophages to shift to an M2 phenotype. Moreover, treatment with conditioned media from decidualized ESCs induced changes in macrophage migration and polarization similar to that of VEGF treatment. These effects were abrogated by the addition of a potent VEGF inhibitor. Together these results suggest that decidual VEGF plays a significant role in macrophage recruitment and M2 polarization, and that inhibition of VEGF signaling may contribute to the shift in macrophage polarity observed in different pregnancy disorders, including preeclampsia.
Carnitine does not improve weight loss outcomes in valproate‐treated bipolar patients consuming an energy‐restricted, low‐fat diet (2006)

At the dose prescribed in this study carnitine supplementation did not improve weight loss outcomes in valproate-treated bipolar patients consuming an energy-restricted, low-fat diet.
An efficient synthesis of some 5-substituted-3-methyl-2-cyclohexen-1-ones using microwaves (2008)

3-Methyl-2-cyclohexenone and its 5-substituted-derivatives are prepared in high yields and short duration of time, using the microwave irradiation technique by the condensation of ethyl acetoacetate with eight different aldehydes and piperazine.
Energy-Efficient Wake-Up Scheduling for Data Collection and Aggregation (2009)

A sensor in wireless sensor networks (WSNs) periodically produces data as it monitors its vicinity. The basic operation in such a network is the systematic gathering (with or without in-network aggregation) and transmitting of sensed data to a base station for further processing. A key challenging question in WSNs is to schedule nodes' activities to reduce energy consumption. In this paper, we focus on designing energy-efficient protocols for low-data-rate WSNs, where sensors consume different energy in different radio states (transmitting, receiving, listening, sleeping, and being idle) and also consume energy for state transition. We use TDMA as the MAC layer protocol and schedule the sensor nodes with consecutive time slots at different radio states while reducing the number of state transitions. We prove that the energy consumption by our scheduling for homogeneous network is at most twice of the optimum and the timespan of our scheduling is at most a constant times of the optimum. The energy consumption by our scheduling for heterogeneous network is at most ¿ (log R <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">max</sub> /R <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">min</sub> ) times of the optimum. We also propose effective algorithms to construct data gathering tree such that the energy consumption and the network throughput is within a constant factor of the optimum. Extensive simulation studies show that our algorithms do considerably reduce energy consumption.
Spectrum optimization in DVB-H single frequency networks with local service areas (2008)

In single frequency networks (SFN) all transmitter make use of the same frequency broadcasting the same data simultaneously within the whole coverage area. Compared to a multi-frequency network (MFN) approach, SFNs offer advantages in terms of coverage and required radio spectrum. However, due to the transmission of equal data, local services are not possible anymore. Using the time slicing technology of digital video broadcasting - handheld (DVB-H), local service distribution can be applied also for SFNs. This approach enables the transmission of global services comprising all SFN advantages, and local service areas (LSAs) with different content by using only one frequency layer. The sizes of the LSAs are limited due to interference and are dependent on the power of the interfering transmitter. This paper deals with the adaption of the transmitting power in order to optimize the sizes of the LSAs. An heuristic optimization approach using simulated annealing (SA) has been proposed. Furthermore, impact of additional frequency layers on LSA and global service area (GSA) coverage will be analyzed. The simulation and the achieved results are based on a realistic scenario.
Periorbital area rejuvenation using carbon dioxide therapy (2012)

Carbon dioxide therapy results as an effective noninvasive modality for the rejuvenation of the periorbital area.
Separation and Recovery of Gallium and Indium from Simulated Zinc Refinery Residue by Liquid−Liquid Extraction (1999)

The separation and recovery of gallium and indium from zinc refinery residue, using liquid−liquid extraction, has been investigated. The major components with the exception of zinc can be removed by extraction with tri-n-butyl phosphate at low aqueous acidity, and gallium and indium can be separated from the remaining major component, zinc, by extraction with bis(2-ethylhexyl)phosphoric acid (D2EHPA) using the correct conditions, at which effective separation may be feasible. Extraction equilibrium formulations for the ternary system containing gallium, indium, and zinc have been established up to high conversion conditions of the extractant to metal complexes, when D2EHPA is employed as the extractant and kerosene as the diluent. The scrubbing behavior for the process can also be expressed by the proposed extraction scheme. Simulation work based on the equilibrium studies shows that indium may be recovered effectively from the mixture of gallium and zinc with 98.9% overall fractional recovery and 100% purity when using 5.0 × 10-2 mol/L D2EHPA. Gallium may then be recovered from zinc with 87.9% overall fractional recovery and 99.1% purity using 1.0 × 10-2 mol/L D2EHPA.
A tunable quantum dissipator for active resonator reset in circuit QED (2018)

Abstract We propose a method for fast, deterministic resonator reset based on tunable dissipative modes. The dissipator is based on a Josephson junction with relatively low quality factor. When the dissipator is tuned into resonance with a high quality microwave resonator, resonator photons are absorbed by the dissipator at a rate orders of magnitude faster than the resonator relaxation rate. We determine the optimal parameters for realization of the tunable dissipator, and examine application of the dissipator to removing spurious photon population in the qubit readout resonator in circuit quantum electrodynamics. We show that even in the nonlinear large photon occupation regime, this enhanced resonator decay rate can be attained by appropriate modulation of the dissipator frequency.
Electroluminescence emission from polariton states in GaAs-based semiconductor microcavities (2008)

The authors report the observation of electroluminescence from GaAs-based semiconductor microcavities in the strong coupling regime. At low current densities, the emission consists of two peaks, which exhibit anticrossing behavior as a function of detection angle and thus originate from polariton states. With increasing carrier injection, we observe a progressive transition from strong to weak coupling due to screening of the exciton resonance by free carriers. The demonstration that polariton emission can be excited by electrical injection is encouraging for future development of polariton lasers.
Single spin states in a defect center resolved by optical spectroscopy (2002)

Individual paramagnetic defect centers in diamond nanocrystals have been investigated by low-temperature high-resolution optical spectroscopy. Narrow fluorescence excitation spectral lines have been found, indicating transitions between individual spin sublevels. Spectral diffusion is explained by cross relaxation among spin sublevels and by the presence of excited electrons in the conduction band of diamond. The relaxation times are in the millisecond range. The system may be useful for quantum information processing with individual electron spins.
Host Chemokines Bind to Staphylococcus aureus and Stimulate Protein A Release (2010)

There are few examples of host signals that are beneficial to bacteria during infection. Here we found that 31 out of 42 host immunoregulatory chemokines were able to induce release of the virulence factor protein A (SPA) from a strain of community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA). Detailed study of chemokine CXCL9 revealed that SPA release occurred through a post-translational mechanism and was inversely proportional to bacterial density. CXCL9 bound specifically to the cell membrane of CA-MRSA, and the related SPA-releasing chemokine CXCL10 bound to both cell wall and cell membrane. Clinical samples from patients infected with S. aureus and samples from a mouse model of CA-MRSA skin abscess all contained extracellular SPA. Further, SPA-releasing chemokines were present in mouse skin lesions infected with CA-MRSA. Our data identify a potential new mode of immune evasion, in which the pathogen exploits a host defense factor to release a virulence factor; moreover, chemokine binding may serve a scavenging function in immune evasion by S. aureus.
THEORY OF CONSTRAINTS SOFTWARE FOR LEAN PRODUCTIONIMPLEMENTATIONS: AN INNOVATION (2008)

Compared to mass production, Lean Production increased
					competitiveness in the automotive industry, by enabling more
					quality with fewer resources. However, it is difficult to implement
					Lean Production in manufacturing environments with high demand
					variability, big product variety, numerous production down times or
					production capacity constraint. This theoretical paper aims to
					define hypothesis about the feasibility to implement Lean
					Production in complex manufacturing environments, by means of the
					software for production planning and control with Theory of
					Constraints' algorithms. The paper reviews concepts about
					innovation, Lean Production evolution, the challenges of
					implementing Lean manufacturing in complex manufacturing
					environments and the Theory of Constraints algorithms. Then, an
					interview with a production planner is presented, who implemented
					Lean Production with the Theory of Constraints software. Finally,
					hypothesis to be validated in future researches are proposed.
Ultra-Low-Loss Optical Delay Line on a Silicon Chip (2011)

Get PDF Email Share Share with Facebook Tweet This Post on reddit Share with LinkedIn Add to CiteULike Add to Mendeley Add to BibSonomy Get Citation Copy Citation Text T. Chen, H. Lee, J. Li, O. Painter, and K. Vahala, "Ultra-Low-Loss Optical Delay Line on a Silicon Chip," in Frontiers in Optics 2011/Laser Science XXVII, OSA Technical Digest (Optica Publishing Group, 2011), paper FWS1. Export Citation BibTex Endnote (RIS) HTML Plain Text Citation alert Save article
IP Flow Information Export (IPFIX) Mediation: Problem Statement (2010)

Flow-based measurement is a popular method for various network monitoring usages.The sharing of flow-based information for monitoring applications having different requirements raises some open issues in terms of measurement system scalability, flow-based measurement flexibility, and export reliability that IP Flow Information Export (IPFIX) Mediation may help resolve.This document describes some problems related to flow-based measurement that network administrators have been facing, and then it describes IPFIX Mediation applicability examples along with the problems.
[Protein fingerprinting as a method for strain-specific differentiation of technologically useful Lactobacillus strains from clinical isolates]. (1994)

Lactobacilli are often used in products of animal origin because of their technological properties and as protective cultures against pathogenic microorganisms, however they are supposed to be related to human infections as well. Therefore 39 strains of species that are in biotechnological usage, including the type strains, and some clinical isolates were investigated. 9 strains of L. acidophilus, 7 of L. gasseri and 23 strains of L. rhamnosus were tested. Biochemical and physiological properties were proved by classical tests. Analysis of the total soluble cytoplasmatic protein patterns was performed with diamine silver staining, a technique not previously applied to lactobacilli. Classical tests were able to confirm all strains as belonging to one of the three species. Analysis of protein patterns allowed to differentiate between strains of the same species. In case of L. acidophilus biotechnologically used strains and clinical isolates were clustered separately. L. rhamnosus formed three clusters. SDS-PAGE of proteins together with diamine silver staining seems to be helpful to detect intra-species differences. It was stated, that technologically used strains of lactobacilli could clearly be differentiated from another and also from clinical isolates.
A Zero Voltage Transition Boost Converter Employing a Soft Switching Auxiliary Circuit With Reduced Conduction Losses (2004)

This paper presents a zero-voltage-transition (ZVT) boost converter using a soft switching auxiliary circuit for power factor correction (PFC) applications. The improvement over existing topologies lies in the positioning of the auxiliary circuit capacitors and the subsequent reduction in the resonant current and therefore the conduction losses as compared to other similar topologies. The proposed converter operates in two modes - Mode 1 and Mode 2. It is shown in the paper that the converter should be designed using the constraints obtained in Mode 1 to achieve low-loss switching. The converter is analyzed and characteristic curves presented which are then used in a detailed design example. Experimental results from a 250 W, 127 V input laboratory prototype switching at 100 kHz verify the design process and highlight the advantages of the proposed topology. The proposed converter is suitable for single-phase, two stage power factor correction circuits with universal input voltage range and power levels up to 3 kW.