gebhart/patrepeval huggingface dataset:
The Amino-terminal Domain of the E Subunit of Vacuolar H+-ATPase (V-ATPase) Interacts with the H Subunit and Is Required for V-ATPase Function (2002)

Vacuolar H(+)-ATPases (V-ATPases) are highly conserved proton pumps that couple hydrolysis of cytosolic ATP to proton transport out of the cytosol. Although it is generally believed that V-ATPases transport protons by a rotary catalytic mechanism analogous to that used by F(1)F(0)-ATPases, the structure and subunit composition of the central or peripheral stalk of the multisubunit complex are not well understood. We searched for proteins that bind to the E subunit of V-ATPase using the yeast two-hybrid assay and identified the H subunit as an interacting partner. Physical association between the E and H subunits of V-ATPase was confirmed in vitro by precipitation assays. Deletion mapping analysis revealed that a 78-amino acid fragment at the amino terminus of the E subunit was sufficient for binding to the H subunit. Expression of the amino-terminal fragments of the E subunits from human and yeast as dominant-negative mutants resulted in dramatic decreases in bafilomycin A(1)-sensitive ATP hydrolysis and proton transport activities of V-ATPase. Our data demonstrate the physiological significance of the interaction between the E and H subunits of V-ATPase and extend previous studies on the arrangement of subunits on the peripheral stalk of V-ATPase.
All-optical AND/NAND logic gates using semiconductor microresonators (2003)

We demonstrate all-optical AND and NAND logic gate operations by nonlinear absorption in a single critically coupled semiconductor micro-racetrack resonator. The device is pumped with two counter-propagating data streams tuned to one of its resonance wavelengths and probed at the next higher resonance wavelength. We investigate and compare the performance of both InP and GaAs devices in terms of switching energy and speed. The GaAs gate demonstrates operation up to 30 Gb/s.
PPARγ helix 12 exhibits an antagonist conformation (2016)

Although the peroxisome proliferator-activated receptor γ (PPARγ) is one of the most studied nuclear receptors (NR), it is still unknown whether its activation helix (helix 12, H12) could exhibit an antagonist conformation as previously demonstrated for most of the NRs. The high H12 flexibility in the apo PPARγ form and the lack of appropriate antagonist ligands complicate the structural and dynamics description by most of the experimental techniques. Based on intensive (≈12 μs) accelerated molecular dynamics (aMD) simulations together with metadynamics and conventional MD runs, we reveal that H12 could exist in an antagonist conformation. This H12 state and the well-known agonist configuration have virtually identical free energy. Notably, significant deviations in the H12 conformations are detected in a homodimer. In chain A the activation helix is stabilized only in a full agonist conformation whereas in chain B, due to agonist to antagonist state exchanges, H12 is oriented toward helix 4. In summary, the results provide an explanation of the observed asymmetry in most of the PPARγ homodimer crystal structures. They also suggest selection guidance for protein moieties and structure candidates that would best serve as potential ligand binding sites to achieve a stable antagonist form of the receptor.
Wave Mixing In Photorefractive Bismuth Silicon Oxide Crystals And Its Applications (1985)

We review the conditions of high gain in wave-mixing experiments with photorefractive Bit 2Si020 crystals. These interactions are based on the recording of a moving interference pattern in the volume of the crystal ("drift" recording mode with an externally applied electric field). This technique has recently resulted in large values of the exponential gain coefficient r = 8 to 12 cm-1 for an optimized grating velocity and grating spacing. Applications to image amplification, laser beam steering, amplified phase conjugation, and self-induced optical cavities are presented.
Long-Term Respiratory and Neurological Sequelae of COVID-19 (2020)

Since the initial reports of coronavirus disease 2019 (COVID-19) in China in late 2019, infections from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have spread rapidly, resulting in a global pandemic that has caused millions of deaths. Initially, the large number of infected people required the direction of global healthcare resources to provide supportive care for the acutely ill population in an attempt to reduce mortality. While clinical trials for safe and effective antiviral agents are ongoing, and vaccine development programs are being accelerated, long-term sequelae of SARS-CoV-2 infection have become increasingly recognized and concerning. Although the upper and lower respiratory tracts are the main sites of entry of SARS-CoV-2 into the body, resulting in COVID-19 pneumonia as the most common presentation, acute lung damage may be followed by pulmonary fibrosis and chronic impairment of lung function, with impaired quality of life. Also, increasing reports have shown that SARS-CoV-2 infection involves the central nervous system (CNS) and the peripheral nervous system (PNS) and directly or indirectly damages neurons, leading to long-term neurological sequelae. This review aims to provide an update on the mechanisms involved in the development of the long-term sequelae of SARS-CoV-2 infection in the 3 main areas of lung injury, neuronal injury, and neurodegenerative diseases, including Alzheimer disease, Parkinson disease, and multiple sclerosis, and highlights the need for patient monitoring following the acute stage of infection with SARS-CoV-2 to provide a rationale for the prevention, diagnosis, and management of these potential long-term sequelae.
Effects of aromatic spacers on the properties of organic field effect transistors based on π-extended tetrathiafulvalene derivatives (2009)

OFETs built from extended TTF derivatives containing N-methylpyrrole, furan, thiophene and benzene with meta or para linkages as spacers are presented. The aromatic spacer plays a crucial role in the characteristics and stability of the OFETs. The less conjugated derivative with a meta-benzene moiety as spacer has the best mobility, with a higher on/off ratio and strongly enhanced stability in air.
Pleiotrophic inhibition of pericellular urokinase-type plasminogen activator system by endogenous tumor suppressive maspin. (2001)

Maspin is a novel serine protease inhibitor with tumor suppressive activity, inhibiting tumor invasion and metastasis. To date, the underlying molecular mechanism of maspin remains elusive. Recombinant maspin has been shown to specifically inhibit cell surface-associated urokinase-type plasminogen activator (uPA) and fibrinogen-bound tissue-type plasminogen activator. However, the role of endogenous maspin in plasminogen activation is totally unknown. To address this issue, we generated stable maspin-expressing transfectants using prostate carcinoma cells DU145 as the parental cell line. We report here that endogenous maspin exerts pleiotropic inhibitory effects on the pericellular uPA system. Maspin expression led to a significantly reduced level of cell surface-bound uPA and uPA receptor proteins without altering the steady-state levels of the respective mRNAs. Treatment with receptor-associated protein (RAP), a specific inhibitor of low-density lipoprotein receptor-related protein, lead to a significantly increased level of secreted uPA and cell surface uPAR in maspin transfectants but not in the mock control cells. A combination of enzymatic and molecular analyses revealed that maspin inhibits the cell surface-mediated plasminogen activation by forming an SDS-resistant complex with cell surface-bound uPA. In addition, maspin expression led to a dramatic reduction in the release of active uPA, both high molecular weight and the low molecular weight, into the conditioned culture medium. Consistently, the conditioned medium of maspin transfectant clones had a significantly reduced activity in converting plasminogen to plasmin. The inhibitory effect of maspin on pericellular uPA correlates with significantly decreased cell invasion potential and motility in vitro. The maspin-neutralizing antibody (Abs4A) reversed the subdued invasive potential of maspin transfectant cells in a dose-dependent manner. In summary, this study provides the first evidence that endogenous maspin is a potent inhibitor of pericellular uPA. Furthermore, our results support a current hypothesis that maspin blocks tumor invasion and motility by inhibiting localized pericellular proteolysis.
Laser Optical Sensor, a Label-Free On-Plate Salmonella enterica Colony Detection Tool (2014)

ABSTRACT We investigated the application capabilities of a laser optical sensor, BARDOT ( b a cterial r apid d etection using o ptical scatter t echnology) to generate differentiating scatter patterns for the 20 most frequently reported serovars of Salmonella enterica . Initially, the study tested the classification ability of BARDOT by using six Salmonella serovars grown on brain heart infusion, brilliant green, xylose lysine deoxycholate, and xylose lysine tergitol 4 (XLT4) agar plates. Highly accurate discrimination (95.9%) was obtained by using scatter signatures collected from colonies grown on XLT4. Further verification used a total of 36 serovars (the top 20 plus 16) comprising 123 strains with classification precision levels of 88 to 100%. The similarities between the optical phenotypes of strains analyzed by BARDOT were in general agreement with the genotypes analyzed by pulsed-field gel electrophoresis (PFGE). BARDOT was evaluated for the real-time detection and identification of Salmonella colonies grown from inoculated (1.2 × 10 2 CFU/30 g) peanut butter, chicken breast, and spinach or from naturally contaminated meat. After a sequential enrichment in buffered peptone water and modified Rappaport Vassiliadis broth for 4 h each, followed by growth on XLT4 (~16 h), BARDOT detected S . Typhimurium with 84% accuracy in 24 h, returning results comparable to those of the USDA Food Safety and Inspection Service method, which requires ~72 h. BARDOT also detected Salmonella (90 to 100% accuracy) in the presence of background microbiota from naturally contaminated meat, verified by 16S rRNA sequencing and PFGE. Prolonged residence (28 days) of Salmonella in peanut butter did not affect the bacterial ability to form colonies with consistent optical phenotypes. This study shows BARDOT’s potential for nondestructive and high-throughput detection of Salmonella in food samples. IMPORTANCE High-throughput screening of food products for pathogens would have a significant impact on the reduction of food-borne hazards. A laser optical sensor was developed to screen pathogen colonies on an agar plate instantly without damaging the colonies; this method aids in early pathogen detection by the classical microbiological culture-based method. Here we demonstrate that this sensor was able to detect the 36 Salmonella serovars tested, including the top 20 serovars, and to identify isolates of the top 8 Salmonella serovars. Furthermore, it can detect Salmonella in food samples in the presence of background microbiota in 24 h, whereas the standard USDA Food Safety and Inspection Service method requires about 72 h.
ISL1 overexpression enhances the survival of transplanted human mesenchymal stem cells in a murine myocardial infarction model (2018)

These results suggest that overexpression of ISL1 in hMSCs promotes cell survival in a model of MI and enhances their paracrine function to protect cardiomyocytes, which may be mediated through IGFBP3. ISL1 overexpression in hMSCs may represent a novel strategy for enhancing the effectiveness of stem cell therapy after MI.
Changes in Renal Function and Oxidative Status Associated with the Hypotensive Effects of Oleanolic Acid and Related Synthetic Derivatives in Experimental Animals (2015)

The results of this study are novel in that they show 1) a correlation between blood pressure reduction and increased urinary Na+ excretion by OA, 2) a more marked MAP reduction in hypertensive animals and 3) a drug-induced decrease in proximal tubule Na+ reabsorption. The results may also be clinically relevant because OA is effective via oral administration.
Improved synthesis of 1,4,5,8,9,12-hexaazatriphenylene (1986)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTImproved synthesis of 1,4,5,8,9,12-hexaazatriphenyleneDonald Z. RogersCite this: J. Org. Chem. 1986, 51, 20, 3904–3905Publication Date (Print):October 1, 1986Publication History Published online1 May 2002Published inissue 1 October 1986https://pubs.acs.org/doi/10.1021/jo00370a032https://doi.org/10.1021/jo00370a032research-articleACS PublicationsRequest reuse permissionsArticle Views2159Altmetric-Citations56LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
On-chip diamond Raman laser (2015)

Synthetic single-crystal diamond has recently emerged as a promising platform for Raman lasers at exotic wavelengths due to its giant Raman shift, large transparency window and excellent thermal properties yielding a greatly enhanced figure-of-merit compared to conventional materials. To date, diamond Raman lasers have been realized using bulk plates placed inside macroscopic cavities, requiring careful alignment and resulting in high threshold powers (~W-kW). Here we demonstrate an on-chip Raman laser based on fully-integrated, high quality-factor, diamond racetrack micro-resonators embedded in silica. Pumping at telecom wavelengths, we show Stokes output discretely tunable over a ~100nm bandwidth around 2-{\mu}m with output powers >250 {\mu}W, extending the functionality of diamond Raman lasers to an interesting wavelength range at the edge of the mid-infrared spectrum. Continuous-wave operation with only ~85 mW pump threshold power in the feeding waveguide is demonstrated along with continuous, mode-hop-free tuning over ~7.5 GHz in a compact, integrated-optics platform.
Modeling scientific theories. On some notions of structuralism (1998)

Defendant la these d'une reference indirecte a la realite pour les theories definies comme modeles d'un point de vue non-affirmatif (non-statement view), l'A. examine la notion structuraliste de theorie-reseau (theory-net) dans le cadre de la theorie des ensembles consideree comme theorie des categories. Definissant la notion de theorie en termes de systeme de modeles et la theoricite en termes d'application pragmatique, d'une part, et distinguant le cas des mondes possibles du monde actuel, d'autre part, l'A. met en evidence l'insuffisance des modeles potentiels au regard des contraintes qu'il a etablies au niveau de la structure conceptuelle de la theorie
Toward Topology Prediction in Zr-Based Microporous Coordination Polymers: The Role of Linker Geometry and Flexibility (2016)

A design strategy based on geometric analysis of linker shape and flexibility is developed in the context of achieving predictable topology of tetratopic-linker based Zr microporous coordination polymers (MCPs). Tetratopic linkers are categorized into tetrahedral, planar square, or planar rectangular groups based on symmetry with an emphasis on linker flexibility. A prediction framework solely based on linker shape and cluster connectivity derived from this strategy is applied to all 18 reported tetratopic-linker based Zr MCPs, and their determined topologies fit well into the scheme. Two new Zr MCPs (UMCM-312 and UMCM-313) are produced using designed linkers based on the strategy to test the robustness of prediction. UMCM-312 contains a biphenyl-core based tetratopic linker to target tetrahedral shape, and UMCM-313 takes advantage of a perylene based tetratopic linker to achieve rectangular shape. The experimentally determined topologies confirm predictions. It is thus demonstrated that the uncertainty of targeting topologies in tetratopic-linker based Zr MCPs can be reduced by accounting for linker shape and flexibility.
[Investigation of a norovirus outbreak through contaminated centralized water supply system]. (2009)

The epidemic was a diarrhea outbreak that might be caused by norovirus through drinking the contaminated tap water A.
Argentivorous Molecules Bearing Two Aromatic Side-Arms: Ag<sup>+</sup>–π and CH−π Interactions in the Solid State and in Solution (2013)

Seven double-armed cyclens bearing two aromatic side-arms, at the 1- and 7-positions of the cyclens, were prepared via three steps from dimethyl 2,2'-iminodiacetate. The X-ray structures of the Ag(+) complexes and Ag(+)-ion-induced (1)H NMR spectral changes suggest that the two aromatic side-arms cover the Ag(+) ions incorporated in the ligand cavities, as if the aromatic ring "petals" catch the Ag(+) ions in the way a real insectivorous plant (Venus flytrap) catches insects, using two leaves. It is also reported that the CH-π interactions between the aromatic side-arms, as well as the Ag(+)-π interactions, are crucial for double- and tetra-armed cyclens to work as argentivorous molecules.
A high-speed digital neural network chip with low-power chain-reaction architecture (1992)

A high-speed digital neural network chip adopts a polyhedric discrimination neuron (PDN) model and low-power chain-reaction (LCR) architecture that can reduce the power dissipation to one-fiftieth or less. The chip contains 832 fully implemented digital synapse units that form 13 neurons on a 10.3-mm*14.1-mm die using 0.8- mu m CMOS technology. The synapse weights are updated using an external computer. A computational speed of 8 billion connections per second (GCPS) is achieved with low 54-mW power dissipation. The forward propagation time is 104 ns. These features make it possible to implement large-scale neural network chips and systems.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
A Low Cost 3D Tracker for Parallax Barrier Display (2007)

We present a novel head tracker for 3D displays that ensures the viewer has a high degree of movement. The tracker is capable of segmenting the foreground objects (viewer) from background objects using their relative distance from the camera. The tracker is a novel 3D CMOS image sensor based on time of flight (TOF) principle using innovating photon gating techniques. The basic feature incorporates real time depth imaging by capturing the shape of a light-pulse front as it is reflected from a three dimensional object. The basic architecture and main building blocks of a real time depth CMOS pixel are described. For this application, we use a stereoscopic type of display using parallax barrier elements that is described as well.
Zinc inhibition of γ-aminobutyric acid transporter 4 (GAT4) reveals a link between excitatory and inhibitory neurotransmission (2005)

gamma-Aminobutyric acid (GABA) transporters (GATs) play an important role in inhibitory neurotransmission by clearing synaptically released GABA and by maintaining low resting levels of GABA in synaptic and extrasynaptic regions. In certain brain regions, vesicular zinc is colocalized and coreleased with glutamate and modulates the behavior of a number of channels, receptors, and transporters. We examined the effect of zinc on expressed GATs (GAT1, GAT2, GAT3, and GAT4) in Xenopus laevis oocytes by using tracer flux and electrophysiological methods. We show that zinc is a potent inhibitor of GAT4 (K(i) of 3 muM). Immunolocalization of GAT4 in the hippocampus revealed dense localization in the CA1 and CA3 regions of the hippocampus, regions which are known to be heavily populated by zinc-containing glutamatergic neurons. The results suggest a physiological role of synaptically released zinc in the hippocampus, because zinc released from hyperactive glutamatergic neurons may simultaneously bring about elevated GABAergic inhibition. Therefore, this mode of zinc function signifies a link between excitatory and inhibitory neurotransmission and may play a neuroprotective role against glutamate-induced excitotoxicity.
The Pricing and Efficiency of Australian Treasury Bond Futures (2014)

This paper examines the efficiency of the Treasury Bond futures market in Australia. We provide a comprehensive explanation of the method used to price, and evaluate efficiency of the 3 and 10 Year Australian Treasury Bond Futures contracts, against underlying bond baskets. Results indicate that the futures contracts exhibit minimal variation from their theoretical value. The average mispricing equates to 1.96 basis points for 3 Year and 1.19 basis points for 10 Year government bond futures contracts. However, during some periods (including the financial crisis of 2008), the bond futures contracts exhibit greater mispricing. Consistent with prior literature, we find a decreasing pattern of mispricing towards expiry, with the futures contract yields and average forward yields of the underlying bonds converging towards expiry. Further analysis reveals that volatility and time to expiry exhibit a significant positive relationship with the absolute level of mispricing.
Opposition: the Politics of Nostalgia (1994)

The Bonapartist régime claimed to have united France and healed the wounds of a decade of revolutionary discord. The seizure of power in Brumaire, it was argued, was justified by the need to restore order and effective government, and to prevent any further degeneration into factionalism. Bonaparte claimed that he stood above political factions, and popular sovereignty was in theory exercised directly through the First Consul, undivided and undiminished. Were these claims legitimate? The lack of any genuine political opposition during most of the Empire certainly makes it appear that order and reconciliation had been achieved.
Short Fiber Reinforced Geopolymer Composites Manufactured by Extrusion (2005)

Geopolymer composites reinforced with short polyvinyl alcohol (PVA) fibers have been manufactured using the extrusion technique. The extruded products were thin plates with 6mm thickness. It was demonstrated that short fiber reinforced geopolymer composites (SFRGCs) could be extruded without additional rheological modifier. Bending tests have been conducted with the extruded samples to investigate their mechanical properties. The fiber failure patterns in SFRGCs with various formulations were examined by scanning electron microscope and energy dispersive x-ray analysis techniques. The experimental results showed that the addition of PVA fiber could largely increase the ductility of SFRGCs, resulting in fiber failure modes changing from brittle to ductile. The effects of varying the amount of fly ash on the flexural behavior of various SFRGCs were also investigated. The SFRGCs incorporating small percentage of fly ash showed higher flexural strengths but smaller defections, while the SFRGCs incorporating a large percentage of fly ash had lower flexural strengths, but larger deflections. This could be attributed to the change of the bonding between fiber and matrix that led the change of failure mode from fiber fracture to fiber pullout.