gebhart/patrepeval huggingface dataset:
Expression of the novel galanin receptor subtype GALR2 in the adult rat CNS: Distinct distribution from GALR1 (1999)

Recent molecular cloning studies by our laboratory and others have identified the existence of a novel rat galanin receptor subtype, GALR2. In the present study, we examined the regional and cellular distribution of GALR2 mRNA in the rat central nervous system (CNS) by in situ hybridization. For comparative purposes, adjacent sections were probed for GALR1 mRNA expression. Our findings indicate that dorsal root ganglia express by far the highest levels of GALR2 mRNA in the rat CNS. Hybridization signal is mainly concentrated over small and intermediate primary sensory neurons. In spinal cord, the large alpha motoneurons of the ventral horn are moderately labeled and several small, but less intensely labeled, cells are scattered throughout the gray matter. In brain sections, the highest levels of GALR2 mRNA are detected in granule cells of the dentate gyrus, in the mammillary nuclei, and in the cerebellar cortex. Moderate levels of GALR2 mRNA are observed in the olfactory bulb, olfactory tubercle, piriform and retrospinal cortices, hypothalamus (namely the preoptic area, arcuate nucleus, and dorsal hypothalamic area), substantia nigra pars compacta, and sensory trigeminal nucleus. Moderate to weak hybridization signal is also present in several other hypothalamic nuclei, specific layers of the neocortex, periaqueductal gray, and several nuclei within the pons and medulla, including locus coeruleus, lateral parabrachial, motor trigeminal, pontine reticular, hypoglossal, vestibular complex, ambiguus, and facial and lateral reticular nuclei. This novel pattern of GALR2 distribution within the rat CNS differs considerably from that of GALR1, suggesting that specific physiologic effects of galanin may be ascribed to the GALR2 galanin receptor subtype.
The Endocannabinoid Reuptake Inhibitor WOBE437 Is Orally Bioavailable and Exerts Indirect Polypharmacological Effects via Different Endocannabinoid Receptors (2018)

Different anandamide (AEA) transport inhibitors show antinociceptive and antiinflammatory effects <i>in vivo</i>, but due to their concomitant inhibition of fatty acid amide hydrolase (FAAH) and overall poor bioavailability, they cannot be used unequivocally to study the particular role of endocannabinoid (EC) transport in pathophysiological conditions <i>in vivo</i>. Here, the potent and selective endocannabinoid reuptake inhibitor WOBE437, which inhibits AEA and 2-arachidonoylglycerol (2-AG) transport, was tested for its oral bioavailability to the brain. WOBE437 is assumed to locally increase EC levels in tissues in which facilitated EC reuptake intermediates subsequent hydrolysis. Given the marked polypharmacology of ECs, we hypothesized to see differential effects on distinct EC receptors in animal models of acute and chronic pain/inflammation. In C57BL6/J male mice, WOBE437 was orally bioavailable with an estimated <i>t</i><sub>max</sub> value of ≤20 min in plasma (C<sub>max</sub> ∼ 2000 pmol/mL after 50 mg/kg, p.o.) and brain (C<sub>max</sub> ∼ 500 pmol/g after 50 mg/kg, p.o.). WOBE437 was cleared from the brain after approximately 180 min. In addition, in BALB/c male mice, acute oral administration of WOBE437 (50 mg/kg) exhibited similar brain concentrations after 60 min and inhibited analgesia in the hot plate test in a cannabinoid CB1 receptor-dependent manner, without inducing catalepsy or affecting locomotion. WOBE437 significantly elevated AEA in the somatosensory cortex, while showing dose-dependent biphasic effects on 2-AG levels in plasma but no significant changes in <i>N</i>-acylethanolamines other than AEA in any of the tissues. In order to explore the presumed polypharmacology mediated via elevated EC levels, we tested this EC reuptake inhibitor in complete Freud's adjuvant induced monoarthritis in BALB/c mice as a model of chronic inflammation. Repetitive doses of WOBE437 (10 mg/kg, i.p.) attenuated allodynia and edema via cannabinoid CB2, CB1, and PPARγ receptors. The allodynia inhibition of WOBE437 treatment for 3 days was fully reversed by antagonists of any of the receptors. In the single dose treatment the CB2 and TRPV1 antagonists significantly blocked the effect of WOBE437. Overall, our results show the broad utility of WOBE437 for animal experimentation for both p.o. and i.p. administrations. Furthermore, the data indicate the possible involvement of EC reuptake/transport in pathophysiological processes related to pain and inflammation.
Progress toward the development of universal influenza vaccines. (2014)

Influenza remains a major problem causing significant morbidity and mortality annually and periodic pandemics with the potential for 10-100 fold increased mortality. Conventional vaccines can be highly effective if generated each year to match currently circulating viruses. Ongoing research focuses on producing cross-protective vaccines that induce T cell and/ or antibody responses specific for highly conserved viral epitopes. The Saint Louis University Center for Vaccine Development (SLUCVD) is highly engaged in multiple efforts to generate universally relevant influenza vaccines.
Chemochromic Hydrogen Leak Detectors (2009)

At NASA, hydrogen safety is a key concern for space shuttle processing. Leaks of any level must be quickly recognized and addressed due to hydrogen s lower explosion limit. Chemo - chromic devices have been developed to detect hydrogen gas in several embodiments. Because hydrogen is odorless and colorless and poses an explosion hazard, there is an emerging need for sensors to quickly and accurately detect low levels of leaking hydrogen in fuel cells and other advanced energy- generating systems in which hydrogen is used as fuel. The device incorporates a chemo - chromic pigment into a base polymer. The article can reversibly or irreversibly change color upon exposure to hydrogen. The irreversible pigment changes color from a light beige to a dark gray. The sensitivity of the pigment can be tailored to its application by altering its exposure to gas through the incorporation of one or more additives or polymer matrix. Furthermore, through the incorporation of insulating additives, the chemochromic sensor can operate at cryogenic temperatures as low as 78 K. A chemochromic detector of this type can be manufactured into any feasible polymer part including injection molded plastic parts, fiber-spun textiles, or extruded tapes. The detectors are simple, inexpensive, portable, and do not require an external power source. The chemochromic detectors were installed and removed easily at the KSC launch pad without need for special expertise. These detectors may require an external monitor such as the human eye, camera, or electronic detector; however, they could be left in place, unmonitored, and examined later for color change to determine whether there had been exposure to hydrogen. In one type of envisioned application, chemochromic detectors would be fabricated as outer layers (e.g., casings or coatings) on high-pressure hydrogen storage tanks and other components of hydrogen-handling systems to provide visible indications of hydrogen leaks caused by fatigue failures or other failures in those systems. In another type of envisioned application, chemochromic detectors of this type could be optoelectronically instrumented for monitoring to provide measured digital indications of color changes indicative of the presence of hydrogen.
Protection of Pneumococcal Infection by Maternal Intranasal Immunization with Pneumococcal Surface Protein A (2011)

Streptococcus pneumoniae is one of the important causative pathogens for both upper and lower respiratory tract infections during childhood. The current study was designed to evaluate the protection against fatal pneumococcal infections during the infant period by maternal immunization with pneumococcal surface protein A (PspA). Four-week-old females BALB/c mice were immunized with PspA and cholera toxin B (CTB) intranasally twice a week for 3 weeks. After mating, the 10-day-old offspring of these mice were intraperitoneally (i.p.) infected with S. pneumoniae to evaluate survival. Anti-PspA-specific IgG antibody was induced in the sera of mother and offspring. The survival times to death after systemic fatal pneumococcal infections were significantly extended among offspring delivered from PspA-immunized mothers than the controls. Current findings suggest that maternal intranasal immunization with PspA is an attractive procedure against pneumococcal infections in early childhood.
Reducing sarcolipin expression mitigates Duchenne muscular dystrophy and associated cardiomyopathy in mice (2017)

Sarcolipin (SLN) is an inhibitor of the sarco/endoplasmic reticulum (SR) Ca<sup>2+</sup> ATPase (SERCA) and is abnormally elevated in the muscle of Duchenne muscular dystrophy (DMD) patients and animal models. Here we show that reducing SLN levels ameliorates dystrophic pathology in the severe dystrophin/utrophin double mutant (mdx:utr <sup>-/-</sup>) mouse model of DMD. Germline inactivation of one allele of the SLN gene normalizes SLN expression, restores SERCA function, mitigates skeletal muscle and cardiac pathology, improves muscle regeneration, and extends the lifespan. To translate our findings into a therapeutic strategy, we knock down SLN expression in 1-month old mdx:utr <sup>-/-</sup> mice via adeno-associated virus (AAV) 9-mediated RNA interference. The AAV treatment markedly reduces SLN expression, attenuates muscle pathology and improves diaphragm, skeletal muscle and cardiac function. Taken together, our findings suggest that SLN reduction is a promising therapeutic approach for DMD.
Generic and automatic address configuration for data center networks (2010)

Data center networks encode locality and topology information into their server and switch addresses for performance and routing purposes. For this reason, the traditional address configuration protocols such as DHCP require huge amount of manual input, leaving them error-prone.In this paper, we present DAC, a generic and automatic Data center Address Configuration system. With an automatically generated blueprint which defines the connections of servers and switches labeled by logical IDs, e.g., IP addresses, DAC first learns the physical topology labeled by device IDs, e.g., MAC addresses. Then at the core of DAC is its device-to-logical ID mapping and malfunction detection. DAC makes an innovation in abstracting the device-to-logical ID mapping to the graph isomorphism problem, and solves it with low time-complexity by leveraging the attributes of data center network topologies. Its malfunction detection scheme detects errors such as device and link failures and miswirings, including the most difficult case where miswirings do not cause any node degree change.We have evaluated DAC via simulation, implementation and experiments. Our simulation results show that DAC can accurately find all the hardest-to-detect malfunctions and can autoconfigure a large data center with 3.8 million devices in 46 seconds. In our implementation, we successfully autoconfigure a small 64-server BCube network within 300 milliseconds and show that DAC is a viable solution for data center autoconfiguration.
Single image fog removal using anisotropic diffusion (2012)

In this study, a novel and efficient fog removal algorithm is proposed. Fog formation is because of attenuation and airlight. Attenuation reduces the contrast and airlight increases the whiteness in the scene. Proposed algorithm uses anisotropic diffusion to recover scene contrast. Simulation results demonstrate that proposed algorithm outperforms prior state-of-the-art algorithms in terms of contrast gain, percentage of number of saturated pixels and computation time. Proposed algorithm is independent of the density of fog and does not require user intervention. It can handle colour as well as grey images. Along with the RGB (red, blue and green) colour model, proposed algorithm can work for HSI (hue, saturation and intensity) model which further reduces the computation. Proposed algorithm has a wide application in tracking and navigation, consumer electronics and entertainment industries.
Cobalt Electrofill for Future Generations of Contacts and Interconnects (2019)

In traditional copper damascene interconnect electroplating baths, three organic additives (accelerator, suppressor, leveler) are used to produce void-free superconformal fill. Here, a different mechanism for electroplating void-free cobalt interconnects is described. Superconformal void-free cobalt fill is generated using a sacrificial oxidant (H <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">+</sup> ) and the addition of a single suppressor-type additive. A differential plating rate on the field and feature bottom can be obtained through process tuning involving waveform, mass transport conditions, pH, and suppressor concentration.
Aflatoxin Contamination in Corn Differs Among Inoculation Techniques (2010)

Aflatoxin research in corn (Zea mays L.) usually requires application of inoculum of Aspergillus flavus to soil or plant ears. The pin-bar vs. side-needle or spray vs. solid material inoculations using A. flavus isolate F3W4 (NRRL 30798) were compared in 2004, 2006, and 2007 using three hybrids in two irrigated experiments each year at Stoneville, MS. Both were planted on a silty clay soil in randomized complete block designs with four replications of treatments. Mature ears inoculated by the pin-bar, side-needle, or spray methods were analyzed for aflatoxin. Ears from controls and solid material inoculum treatments were sampled for analysis at plot harvest. Pin-bar inoculation had more aflatoxin in 2004 (551.9 ng/g) and 2006 (305.8 ng/g) than side-needle inoculation (342.2 ng/g and 151.1 ng/g for 2004 and 2006, respectively), which was greater than controls (76.8 ng/g and 21.6 ng/g for 2004 and 2006, respectively). Solid material inoculation did not differ in aflatoxin from controls. Spraying produced the most aflatoxin (344.1 ng/g) only in 2004. Aflatoxin was low in 2007 when timely rainfall, irrigation, and no temperatures ≥ 35°C resulted in only the pin-bar (20.8 ng/g) and solid material (20.6 ng/g) treatments having > 2.0 ng/g of aflatoxin. Accepted for publication 26 March 2010. Published 1 June 2010.
Spatial and temporal gradients of paired Gabor filters predict movement discrimination (1987)

This study found that both global and local contrast differences are used to discriminate left-right movement. Movement discrimination was examined by measuring an observer’s ability to discriminate shifts in the position of contrast differences over time for a large range of background contrasts, spatial frequencies, and exposure durations. The contrasts needed to discriminate between two test patterns separated in time by a short interval and shifted in position optimally relative to a multifrequency background were measured using two alternative forced-choice procedures. Multifrequency backgrounds determined the window of visibility used to discriminate movement. A neural network model implemented by the weighted difference and sum of the output of paired odd- and even-symmetric simple and complex cells (the spatial and temporal gradients of paired Gabor filters) pooled across the window of visibility predicts horizontal movement discrimination.
Serodiagnosis of swine pleuropneumonia due to Actinobacillus pleuropneumoniae serotypes 7 and 4 using long-chain lipopolysaccharides. (1997)

A saline boiled extract (SBE), capsular polysaccharides (CPS) and long-chain lipopolysaccharides (LC-LPS) of Actinobacillus pleuropneumoniae serotype 7 have been evaluated in ELISA for the serodiagnosis of swine pleuropneumonia caused by this serotype. Mean optical densities (ODs) obtained with the 3 antigens using sera from negative herds as well as from animals experimentally and naturally exposed to A. pleuropneumoniae serotypes 7 or 4 were not statistically different. The positive ELISA reaction with anti-serotype 4 sera was unexpected with the CPS, which are supposed to be serotype-specific; LPS traces present in the CPS appeared to be responsible for this reaction. In addition, sera from animals exposed to A. pleuropneumoniae serotypes 5 or 10 presented cross-reactions with the SBE and the CPS, but not with the LC-LPS. Cross-reactions were mainly due to rough LPS, as shown by immunoblotting. The LC-LPS is easily obtainable and can be used for the detection of antibodies in animals infected with A. pleuropneumoniae serotypes 7 and 4.
Automatic segmentation of pathological lung using incremental nonnegative matrix factorization (2015)

Accurate segmentation of pathological lungs from large-size chest computed tomographic images is crucial for computer-assisted lung cancer diagnostics. In this paper, a new framework for automatic pathological lung segmentation is proposed. The proposed INMF-based segmentation approach has the ability to handle the in-homogeneities caused by the arteries, veins, bronchi, and possible pathologies that may exist in the lung tissues, and to detect the number of clusters in the image in an automated manner. The proposed INMF-based segmentation framework is quantitatively validated on simulated realistic lung phantoms that mimic different lung pathologies (7 datasets), in vivo data sets for 17 subjects, and for lung disease with severe pathologies. Three metrics are used: the Dice coefficient, modified Hausdorff distance, and absolute lung volume difference. Results show that the proposed approach outperforms existing lung segmentation techniques and can handle in-homogenities caused by different pathologies.
Quasi-soliton propagation in dispersion-managed optical fibers (1997)

Combination of programmed chirp and dispersion profiles produced a solitonlike nonlinear pulse with a stationary structure (quasi-soliton). The quasi-soliton has more attractive properties than those of a soliton because of its reduced interaction and smaller peak power than a soliton and allows ~100-Gbit / s transmission over a 125-dispersion distance with reasonable power.
Abstract 1214: RET fusion gene: translation to the therapy of lung adenocarcinoma. (2013)

Abstract A considerable proportion of lung adenocarcinomas, the most common histological type of lung cancer that comprises 40% of the total cases, develops through activation of oncogenes, for example, somatic mutations in EGFR or KRAS or fusion of ALK, in a mutually exclusive manner. Tyrosine kinase inhibitors targeting the EGFR and ALK proteins are effective in the treatment of lung adenocarcinomas that carry EGFR mutations and ALK fusions, respectively. We performed whole-transcriptome sequencing (RNA sequencing) of 30 lung adenocarcinoma specimens from Japanese individuals to identify new chimeric fusion transcripts that could be targets for therapy. Then, we identified KIF5B (kinesin family 5B)-RET fusion gene, as another druggable oncogene that is present in 2% (6/319) of lung adenocarcinomas (Kohno et al., Nat Med, 2012). The KIF5B-RET fusion leads to aberrant activation of RET kinase and is considered to be a new driver mutation segregating from mutations or fusions in EGFR, KRAS, HER2 and ALK. The RET fusion has preferentially been detected in well or moderately differentiated tumors of never smokers. The RET fusion in lung adenocarcinoma was also identified by three other groups: Dr. Ju et al of Seoul National University, Dr. Takeuchi et al of Japanese Foundation for Cancer Research, and Dr. Lipson et al of Foundation Medicine, USA. A clinical trial to address the therapeutic efficacy of a RET tyrosine kinase inhibitor, vandetanib, will be started in 2013 by Dr. Koichi Gotoh (kgoto@east.ncc.go.jp) of National Cancer Center Hospital East, Japan. Citation Format: Takashi Kohno, Hitoshi Ichikawa, Koji Tsuta, Tatsuji Mizukami, Yoko Shimada, Mamoru Kato, Hiromi Sakamoto, Katsuya Tsuchihara, Shun-ichi Watanabe, Hiroshi Nokihara, Koichi Goto, Jun Yokota, Teruhiko Yoshida, Tatsuhiro Shibata. RET fusion gene: translation to the therapy of lung adenocarcinoma. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 1214. doi:10.1158/1538-7445.AM2013-1214
Performance of Two Novel Fast Affine Projection Adaptation Algorithms (2005)

This paper studies the performance of two recently proposed stable and robust fast affine projection (FAP) adaptation algorithms - LDL/sup T/ FAP and RATCHET FAP. They can, respectively, provide an exact solution at a moderate complexity and a good approximation at a complexity lower than that of any other FAP algorithms. These patent pending algorithms are expected to be practical in telecommunication and other adaptive filtering applications.
Cocaine and 3.beta.-(4'-Substituted phenyl)tropane-2.beta.-carboxylic Acid Ester and Amide Analogs. New High-Affinity and Selective Compounds for the Dopamine Transporter (1995)

Several 2 beta-carboxylic acid ester and amide analogues of cocaine and of 3 beta-(4'-substituted phenyl)tropane-2 beta-carboxylic acid were prepared. The binding affinities of these compounds, and of some previously prepared analogues, at the dopamine (DA), norepinephrine (NE), and serotonin (5-HT) transporters were determined. The phenyl esters of 3 beta-(4'-methylphenyl)- and 3 beta-(4'-chlorophenyl)tropane-2 beta-carboxylic acid are highly potent and highly selective for the DA transporter. The isopropyl esters of 3 beta-(4'-chlorophenyl)- and 3 beta-(4'-iodophenyl)tropane-2 beta-carboxylic acid also possess high DA affinity and show significant DA transporter selectivity. Similarly, the phenyl and isopropyl ester analogues of cocaine are much more selective for the DA transporter than cocaine. Tertiary amide analogues of cocaine and of 3 beta-(4'-substituted phenyl)tropane-2 beta-carboxylic acids are more potent inhibitors of radioligand binding at the DA transporter than the primary and secondary amide analogues. In particular, 3 beta-(4'-chlorophenyl)tropane-2 beta-N-morpholinocarboxamide as well as the 3 beta-(4'-chlorophenyl)- and 3 beta-(4'-iodophenyl)tropane-2 beta-N- pyrrolidinocarboxamides possess high affinity and selectivity for the DA transporter. The N,N-dimethylamide cocaine analogue is the most selective cocaine amide derivative for the DA transporter. High correlation between the inhibition of radioligand binding and inhibition of uptake at the DA, NE, and 5-HT transporter was found for a selected group of analogues. Within this group, one compound, the isopropyl ester of 3 beta-(4'-iodophenyl)-tropane-2 beta-carboxylic acid, was found to be more potent in the inhibition of radioligand binding than in the inhibition of DA uptake. Taken together with its high potency and selectivity at the DA transporter, this suggests that this compound may be a lead in the development of a cocaine antagonist.
A New Method for Cardiac Computed Tomography Regional Function Assessment (2012)

We present a new quantitative method for measuring regional cardiac function from high-resolution volumetric CT images, which can be acquired during angiography and myocardial perfusion scans. Quantified measures of regional cardiac mechanics in normal and abnormally contracting regions in infarcted hearts were shown to correspond well with noninfarcted and infarcted regions as detected by delayed enhancement cardiovascular magnetic resonance images.