gebhart/patrepeval huggingface dataset:
Bond Coat Development for Thermal Barrier Coatings (1989)

The use of thermal barrier coatings on high pressure turbine components can improve gas turbine efficiency through reduction of cooling airflow. However, to reduce cooling airflow, a highly reliable thermal barrier coating is required. This increased reliability will be achievable through several complimentary approaches: material and process development, life prediction method development and engine service experience. The results of bond coat material development which has increased the thermal cycle life of plasma spray thermal barrier coatings are presented. Improvements were achieved by two methods; 1) use of creep resistant bond coat compositions and 2) overaluminiding of the bond coat.
<title>Lasing in Ne-like S and other new developments in capillary discharge ultrashort wavelength lasers</title> (1997)

We report our most recent progress in the development of capillary discharge soft x-ray lasers. This includes the first observation of discharge-pumped ultrashort wavelength lasing in a material that is solid at room temperature (S), and preliminary results of discharges in Ca. Excitation by a capillary discharge of S vapor generated by discharge ablation of a solid target resulted in amplification in Ne-like S at 60.8 nm with a gain-length product of 7.5. Overheating of the electron temperature respect to steady-state ionization conditions and transient population effects significantly increased the gain above the steady state-value. The results of two-dimensional near-field and far-field imaging of a saturated table-top Ne-like Ar laser and the measurement of its spatial coherence as a function of amplifier length are also reported and compared with model calculations. The generation of a capillary discharge plasma waveguide is to be used in combination with ultrashort pulse laser excitation for the generation of a new kind of efficient collisional soft x- ray laser is discussed.
Theory of quasioptical gyrotrons and gyroklystrons operating at higher harmonics of the cyclotron frequency (1983)

A multimode, non-linear, time-dependent formulation of the theory of quasioptical electron cyclotron masers operating at high gyrofrequency harmonics is presented. Linear calculations of the starting current and numerical analyses of the efficiency of the devices are performed. It is shown that with suitable contouring of the DC magnetic field, a stable single mode operation at the second harmonic is possible. To improve the efficiency, an alternative configuration, the quasioptical gyroklystron, is also analysed using single mode and multimode dynamics simulations. The gyroklystron operating at the second harmonic is shown to lead to a significant increase in efficiency (approximately 30%).
Advanced Glycation End Products of DNA: Quantification of <i>N</i><sup>2</sup>-(1-Carboxyethyl)-2′-deoxyguanosine in Biological Samples by Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry (2008)

Methylglyoxal (MG) and related alpha-oxoaldehydes react with proteins, lipids, and DNA to give rise to covalent adducts known as advanced glycation end products (AGEs). Elevated levels of AGEs have been implicated in the pathological complications of diabetes, uremia, Alzheimer's disease, and possibly cancer. There is therefore widespread interest in developing sensitive methods for the in vivo measurement of AGEs as prognostic biomarkers and for treatment monitoring. The two diastereomeric MG-DNA adducts of N(2)-(1-carboxyethyl)-2'-deoxyguanosine (CEdG) are the primary glycation products formed in DNA; however, accurate assessment of their distribution in vivo has not been possible since there is no readily available quantitative method for CEdG determination in biological samples. To address these issues, we have developed a sensitive and quantitative liquid chromatography electrospray ionization tandem mass spectrometry assay using the stable isotope dilution method with an (15)N(5)-CEdG standard. Methods for CEdG determination in urine or tissue extracted DNA are described. Changes in urinary CEdG in diabetic rats in response to oral administration of the AGE inhibitor LR-90 are used to demonstrate the potential utility of the method for treatment monitoring. Both stereoisomeric CEdG adducts were detected in a human breast tumor and normal adjacent tissue at levels of 3-12 adducts/10(7) dG, suggesting that this lesion may be widely distributed in vivo. Strategies for dealing with artifactual adduct formation due to oxoaldehyde generation during DNA isolation and enzymatic workup procedures are described.
Image registration and perfusion imaging: Application to dynamic circular cardiac CT (2010)

Large area detector computed tomography systems with fast rotating gantries enable volumetric dynamic cardiac perfusion studies. Prospectively ECG-triggered acquisitions limit the data acquisition to a predefined cardiac phase and thereby reduce X-ray dose and limit motion artifacts. Even in the case of highly accurate prospective triggering and stable heart rate, spatial misalignment of the cardiac volumes acquired and reconstructed per cardiac cycle may occur due to small motion pattern variations from cycle to cycle. These misalignments reduce the accuracy of the quantitative analysis of myocardial perfusion parameters on a per voxel basis. An image based solution to this problem is elastic 3D image registration of dynamic volume sequences with variable contrast, as it is introduced in this contribution. After circular cone-beam CT reconstruction of cardiac volumes covering large areas of the myocardial tissue, the data set with maximum contrast enhancement is selected as reference data set. The complete series is aligned with respect to this reference volume. The results are evaluated on pig data comparing perfusion measures using the non-registered versus the registered data set. The reduced spatial misalignment leads to an improved characterization of myocardial perfusion confirming the potential of this method.
Structural investigations and biological activity of inositol sphingophospholipids from <i>Phytophthora capsici</i> (1990)

Inositol sphingophospholipids that protect pepper (Capsicum annuum c.v. Yolo Wonder) against pathogen have been isolated by chromatographic methods from the mycelium of Phytophthora capsici. The structure of the major compound was determined by chemical methods and mass spectrometry. Phosphodiester bond cleavage of the phospholipid by mild alkaline hydrolysis liberated a ceramide which contained a C16-sphingosine. This long-chain base was identified by gas chromatography and mass spectrometry of its trimethylsilyl derivative. One of the amide-linked fatty acids was found to be 4-hydroxy-2 docosenoic acid. Fast-atom-bombardment mass spectrometry and fast-atom-bombardment collison-induced tandem mass spectrometry were used to characterize the ceramide as N(4-hydroxy-2-docosenoyl)C16-sphingosine. These sphingolipids have a protective effect on cotyledons of young peppers against necrotic lesions induced by the pathogen P. capsici.
8.4: Blue Emitting Barium Thioaluminate Phosphors by Sputtering of Composite Targets (2005)

Abstract A process is developed for fabrication of thin film Ba‐Al‐S: Eu phosphors for inorganic electroluminescent FPD. It includes sputter‐deposition from a single composite target and in‐situ anneal of the phosphor films. The phosphors exhibit EL‐luminance up to 900 cd/m 2 and lifetime sufficient for TV applications.
Association of porcine circovirus 2 with reproductive failure in pigs: a retrospective study, 1995-1998. (2001)

In order to determine if vertically transmitted porcine circovirus (PCV) has played a role in reproductive failure in pigs in areas of endemic infection, archival fixed tissues were examined by polymerase chain reaction (PCR) and immunohistochemistry. Tissues tested were from routine cases of abortion or reproductive failure submitted between 1995 and 1998 to the diagnostic laboratory at the Western College of Veterinary Medicine, Saskatoon. They originated from 29 high-health herds in the provinces of Alberta and Saskatchewan and comprised a total of 36 individual submissions. Porcine circovirus type 1 (PCV1) was not detected by PCR in any submitted tissues. Porcine circovirus type 2 (PCV2) was not detected by PCR or immunohistochemistry in any of the submitted tissue. The effect of extended formalin fixation on the detection of PCV2 by PCR was assessed and fixation for up to one week had no gross effect on sensitivity of detection using this PCR technique. Failure to detect porcine circoviruses in cases of reproductive failure prior to 1999 in areas of endemic infections, suggests that reproductive disease may be a new clinical manifestation of PCV2 infection, and that vertical transmission may not have been the primary mechanism of initial dissemination of the virus in the pig population.
Cross-talk between Integrin α6β4 and Insulin-like Growth Factor-1 Receptor (IGF1R) through Direct α6β4 Binding to IGF1 and Subsequent α6β4-IGF1-IGF1R Ternary Complex Formation in Anchorage-independent Conditions (2012)

Integrin αvβ3 plays a role in insulin-like growth factor-1 (IGF1) signaling (integrin-IGF1 receptor (IGF1R) cross-talk). The specifics of the cross-talk are, however, unclear. In a current model, "ligand occupancy" of αvβ3 (i.e. the binding of extracellular matrix proteins) enhances signaling induced by IGF1 binding to IGF1R. We recently reported that IGF1 directly binds to αvβ3 and induces αvβ3-IGF1-IGF1R ternary complex formation. Consistently, the integrin binding-defective IGF1 mutant (R36E/R37E) is defective in inducing ternary complex formation and IGF signaling, but it still binds to IGF1R. Like αvβ3, integrin α6β4 is overexpressed in many cancers and is implicated in cancer progression. Here, we discovered that α6β4 directly bound to IGF1, but not to R36E/R37E. Grafting the β4 sequence WPNSDP (residues 167-172), which corresponds to the specificity loop of β3, to integrin β1 markedly enhanced IGF1 binding to β1, suggesting that the WPNSDP sequence is involved in IGF1 recognition. WT IGF1 induced α6β4-IGF1-IGF1R ternary complex formation, whereas R36E/R37E did not. When cells were attached to matrix, exogenous IGF1 or α6β4 expression had little or no effect on intracellular signaling. When cell-matrix adhesion was reduced (in poly(2-hydroxyethyl methacrylate-coated plates), IGF1 induced intracellular signaling and enhanced cell survival in an α6β4-dependent manner. Also IGF1 enhanced colony formation in soft agar in an α6β4-dependent manner. These results suggest that IGF binding to α6β4 plays a major role in IGF signaling in anchorage-independent conditions, which mimic the in vivo environment, and is a novel therapeutic target.
Five-Hour Diagnosis of Dermatophyte Nail Infections with Specific Detection of<i>Trichophyton rubrum</i> (2007)

A rapid two-step DNA extraction method and a multiplex PCR for the detection of dermatophytes in general and Trichophyton rubrum specifically were developed and evaluated with DNA extracted from pure cultures and from clinically diseased nails. DNA from the following dermatophytes was used: Epidermophyton floccosum, Microsporum audouinii, Microsporum canis, Microsporum gypseum, Microsporum nanum, Trichophyton mentagrophytes, Trichophyton rubrum, Trichophyton schoenleinii, Trichophyton soudanense, Trichophyton terrestre, Trichophyton tonsurans, Trichophyton verrucosum, and Trichophyton violaceum. Human DNA and DNA from the following nondermatophyte fungi were included as controls: Alternaria, Aspergillus niger, Candida albicans, Candida glabrata, Candida krusei, Malassezia furfur, Saccharomyces cerevisiae, and Scopulariopsis brevicaulis. A total of 118 nail samples received for routine microscopy and culture for dermatophytes were subsequently tested by the two PCRs separately and in a multiplex format. Using DNA extracted from pure cultures and the pan-dermatophyte PCR, the T. rubrum-specific PCR sequentially and in a multiplex format correctly detected all dermatophytes and additionally correctly identified T. rubrum. Comparison of the traditional diagnostic evaluation (microscopy and culture) of nail samples with PCR on DNA directly extracted from the nails showed excellent agreement between PCR and microscopy, but the number of samples with dermatophyte species identification was increased considerably from 22.9% to 41.5%, mainly due to the identification of T. rubrum by PCR in microscopy-positive but culture-negative samples. In conclusion, this 5-hour diagnostic test was shown to increase not only the speed but also the sensitivity of investigation for nail dermatophytosis.
Stand-off detection of trace explosives via resonant infrared photothermal imaging (2008)

We describe a technique for rapid stand-off detection of trace explosives and other analytes of interest. An infrared (IR) laser is directed to a surface of interest, which is viewed using a thermal imager. Resonant absorption by the analyte at specific IR wavelengths selectively heats the analyte, providing a thermal contrast with the substrate. The concept is demonstrated using trinitrotoluene and cyclotrimethylenetrinitramine on transparent, absorbing, and reflecting substrates. Trace explosives have been detected from particles as small as 10 μm.
Compact, frequency-agile, absorptive bandstop filters (2005)

Two absorptive bandstop filter circuit topologies are introduced. The preferred topology is analyzed and its advantages over traditional approaches quantified. A corresponding varactor-tuned microstrip absorptive bandstop filter is described and its unique ability to maintain its attenuation while tuning across a broad frequency range using only resonant frequency tuning is demonstrated.
Test-Time Detection of Backdoor Triggers for Poisoned Deep Neural Networks (2022)

Backdoor (Trojan) attacks are emerging threats against deep neural networks (DNN). A DNN being attacked will predict to an attacker-desired target class whenever a test sample from any source class is embedded with a backdoor pattern, while correctly classifying clean (attack-free) test samples. Existing backdoor defenses have shown success in detecting whether a DNN is attacked and in reverse-engineering the backdoor pattern in a "post-training" scenario: the defender has access to the DNN to be inspected and a small, clean dataset collected independently, but has no access to the (possibly poisoned) training set of the DNN. However, these defenses neither catch culprits in the act of triggering the backdoor mapping, nor mitigate the backdoor attack at test-time. In this paper, we propose an "in-flight" unsupervised defense against backdoor attacks on image classification that 1) detects use of a backdoor trigger at test-time; and 2) infers the class of origin (source class) for a detected trigger example. The effectiveness of our defense is demonstrated experimentally for a wide variety of DNN architectures, datasets, and backdoor attack configurations.
Identification and In Vitro Reactivity of Celiac Immunoactive Peptides in an Apparent Gluten-Free Beer (2014)

Gluten content from barley, rye, wheat and in certain oat varieties, must be avoid in individuals with celiac disease. In most of the Western countries, the level of gluten content in food to be considered as gluten-free products is below 20 parts per million measured by ELISA based on specific anti-gluten peptide antibody. However, in beverages or food suffering complex hydrolytic processes as beers, the relative proportion of reactive peptides for celiac patients and the analytical techniques may differ, because of the diversity of the resulting peptide populations after fermentations. A beer below 20 parts per million of gluten but yet detectable levels of gluten peptides by anti-gliadin 33-mer antibodies (G12 and A1) was analyzed. We identified and characterized the relevant peptides for either antibody recognition or immunoactivity in celiac patients. The beer was fractionated by HPLC. The relative reactivity of the different HPLC fractions to the G12/A1 antibodies correlated to the reactivity of peripheral blood mononuclear cells isolated from 14 celiac individuals. Peptides from representative fractions classified according to the relative reactivity to G12/A1 antibodies were identified by mass spectrometry. The beer peptides containing sequences with similarity to those of previously described G12 and A1 epitopes were synthesized and confirmed significant reactivity for the antibodies. The most reactive peptides for G12/A1 also confirmed the highest immunogenicity by peripheral blood mononuclear cell activation and interferon γ production from celiac patients. We concluded that preparative HPLC combined with anti-gliadin 33-mer G12/A1 antibodies were very sensitive and specific methods to analyze the relevant immunogenic peptides in hydrolyzed gluten.
Automatic Detection of Conducted Premature Atrial Contractions to Predict Atrial Fibrillation in Patients after Cardiac Surgery (2004)

A method was developed to automatically detect conducted Premature Atrial Contractions (PACs) from the ECG signal. The method consisted of idenrifying the R waves of the ECG signal, determining the irregular beats that correspond to premature beats and dissociating atrial and ventricular premature beats. The method was developed using the ECG signals from Physionet and Cardio- Vascular Intensive Care Units (CVICUs) at the Cleveland Clinic. The developed method was applied on ECG signals collected from 24 ~ post-cardiovascular surgery patients that developed AFIB. Out of the 24 patients, 21 patients had significant number of PACs before AFIB. Additionally, in these patients either a paltern of sustained cyclical PAC activity or bursts of iniermittenf PAC activity were observed.
Nucleotide sequences and phylogeny of the nucleocapsid gene of Oropouche virus (2000)

The nucleotide sequence of the S RNA segment of the Oropouche (ORO) virus prototype strain TRVL 9760 was determined and found to be 754 nucleotides in length. In the virion-complementary orientation, the RNA contained two overlapping open reading frames of 693 and 273 nucleotides that were predicted to encode proteins of 231 and 91 amino acids, respectively. Subsequently, the nucleotide sequences of the nucleocapsid genes of 27 additional ORO virus strains, representing a 42 year interval and a wide geographical range in South America, were determined. Phylogenetic analyses revealed that all the ORO virus strains formed a monophyletic group that comprised three distinct lineages. Lineage I contained the prototype strain from Trinidad and most of the Brazilian strains, lineage II contained six Peruvian strains isolated between 1992 and 1998, and two strains from western Brazil isolated in 1991, while lineage III comprised four strains isolated in Panama during 1989.
Scanning electrochemical microscopy. Introduction and principles (1989)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTScanning electrochemical microscopy. Introduction and principlesAllen J. Bard, Fu Ren F. Fan, Juhyoun. Kwak, and Ovadia. LevCite this: Anal. Chem. 1989, 61, 2, 132–138Publication Date (Print):January 15, 1989Publication History Published online1 May 2002Published inissue 15 January 1989https://pubs.acs.org/doi/10.1021/ac00177a011https://doi.org/10.1021/ac00177a011research-articleACS PublicationsRequest reuse permissionsArticle Views5838Altmetric-Citations971LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Core Dominated Surface Activity of Core–Shell Nanocatalysts on Methanol Electrooxidation (2012)

The activity of core–shell nanoparticles (NCs) in electrooxidation of methanol (MOR) was found to be dependent on the crystalline structure of the core and the lattice strain at the core–shell interface. Ru-core and Pt-shell NCs delivered 6.1-fold peak MOR current density at −135 mV than Pt NCs, while the Co-core and Pt-shell NCs showed a 1.4-fold peak MOR current density at 280 mV. The current density is improved by the compressive lattice strain of the surface that is caused by the lattice mismatch between the Pt shell and the Ru core. For Co-core NCs, the enhancement results from the ligand effect at surface Pt sites. In addition, the Ru-core NCs maintained a steady current density of 0.11 mA cm–2 at 500 mV in a half-cell system for 2 h, which is 100-fold higher than that of Pt NCs and Co-core NCs. These results provide mechanistic information for the development of fuel cell catalysts along with reduced Pt utilization and programmable electrochemical performance.
Production of high quality and natural-like recombinant allergens needed for high efficiency of allergy treatment (2014)

Background Due to a lack of sufficiently accurate and effective tools, allergy is often badly diagnosed. Moreover, its treatment is expensive, long, uncomfortable and of little effectiveness. In this context, less than a quarter of allergic patients receive a treatment currently. Allergies have therefore become an important public health issue, highlighting the many unmet medical needs. ANGANY Genetics has brought together expertise in protein genomics and in therapeutic protein production in plant expression systems, to produce recombinant allergens of an unprecedented quality, offering the following solutions to allergy sufferers: (1) a precise identification of each allergen involved rather than the current approximate diagnosis, (2) pure and certified recombinant allergens rather than imperfect and incomplete allergen extracts and (3) an effective treatment at the best cost to replace symptomatic-only or low-level curative allergy treatments.