gebhart/patrepeval huggingface dataset:
A Trichomonas vaginalis cDNA with partial protein sequence homology with a Plasmodium falciparum excreted protein ABRA. (1993)

We have previously isolated two extracellular cysteine proteases (60 kDa, 30 kDa) from the cell filtrate of an isolate of Trichomonas vaginalis. All isolates tested produced a 60 kDa protease as demonstrated by immunoblot with cross-reacting rabbit serum. A T. vaginalis cDNA library was constructed using lambda gt11. A 760 bp T. vaginalis specific cDNA was sequenced and demonstrates partial protein sequence homology with an extracellular cysteine protease of Plasmodium falciparum. These results are consistent with the hypothesis that this protease may be important in the pathogenesis of T. vaginalis infection.
Antisense‐inhibition of ADP‐glucose pyrophosphorylase in developing seeds of <i>Vicia narbonensis</i> moderately decreases starch but increases protein content and affects seed maturation (2000)

The small subunit of a Vicia faba ADP-glucose pyrophosphorylase (AGP) cDNA was expressed in antisense orientation in Vicia narbonensis under the control of the seed-specific legumin B4 promoter. From several independent transgenic lines both ADP-glucose pyrophosphorylase AGP-mRNA and AGP enzyme activity were reduced by up to 95% in the cotyledons during the mid- to late-maturation phase. Starch was moderately decreased and sucrose was increased. In two of three lines, transcripts encoding the large subunit of AGP and the storage protein vicilin were increased, whereas legumin B-mRNA was decreased. Transcripts of other storage-associated genes were not altered. The cotyledons contained more protein and total nitrogen. Despite the reduction in starch, total carbon was not decreased and dry weight was unchanged. Compared to the wild type, transgenic seeds contained more water and accumulated dry weight during a longer period, and therefore had a prolonged seed-filling period. Transgenic cotyledon cells of comparable age to the wild type were more highly vacuolated and contained smaller starch grains, indicating a delay in cellular differentiation. We conclude that a specific alteration in carbon metabolism can have pleiotropic effects on water and nitrogen content and induces temporal changes in seed development.
Pretiree lifestyles in relation to musculoskeletal health: cross‐sectional data from the Geelong Osteoporosis Study (2018)

Abstract Aims What happens in the early‐elderly ‘pretiree’ period potentially influences the divergent paths of healthy or unhealthy ageing. In this cross‐sectional study, we aimed to profile musculoskeletal health and lifestyle behaviours for men and women in their late‐fifties and sixties. Methods and results For 482 participants from the Geelong Osteoporosis Study, we measured DXA‐derived relative appendicular lean mass (rALM), bone mineral density (BMD) at the femoral neck and percentage body fat mass (%BF). Low‐rALM and low‐BMD referred to sex‐specific T‐scores<‐1.0. Associations between exposures and low‐rALM and/or low‐BMD were explored using multivariable logistic regression. Three‐quarters of participants had high %BF, 98(20.3%) had low‐rALM, 202(41.9%) had low‐BMD and 63(13.1%) had both low‐rALM and low‐BMD. Eight‐two (17.0%) were very active and one‐third participated in sports/recreational activities. Most [n=416(87.8%)] met the recommended daily intake (RDI) for protein; only 119(25.1%) met the RDI for calcium. Less than 10% smoked and one‐third exceeded recommended alcohol intakes. Independent of age, weight and sex, greater %BF and sedentary behaviour increased the likelihood of low‐rALM; high‐alcohol consumption increased the likelihood of low‐BMD; and greater %BF increased the likelihood of low‐rALM and low‐BMD combined. Conclusions One‐half of participants had rALM and BMD in the normal range. Only a few were involved in resistance‐training or weight‐bearing exercise, despite having the capacity to be physically active. As sedentary lifestyles, excessive adiposity and high alcohol use were associated with low‐rALM and/or low‐BMD, we propose that these adverse factors be potential targets among pretirees to minimise their risk of entering old age with poor musculoskeletal health.
Real-Time NMR Studies of Electrochemical Double-Layer Capacitors (2011)

(11)B NMR spectroscopy has been used to investigate the sorption of BF(4)(-) anions on a highly porous, high surface area carbon, and different binding sites have been identified. By implementing in situ NMR approaches, the migration of ions between the electrodes of the supercapacitors and changes in the nature of ion binding to the surface have been observed in real time.
A Solution of Electronic Authentication Services Based on PKI for Enabling e-Business (2009)

As the basic application of PKI (Public Key Infrastructure) theories and technologies, electronic authentication services (thereinafter referred to as EAS) is defined as to provide authentic identities and identity verification services for participants involved in network-based activities. Due to its complexity of constructing PKI, most application systems including e-business platforms need to integrate EAS provided by a third party into their own business processes. Based on exploration on the requirements of EAS for e-business together with careful study and engineering practice in this area, this paper presents a complete solution of EAS based on PKI for enabling e-business. Architecture of EAS and its functionality components are brought forth and illustrated first. Then we brought forward a cross-domain trust model and method of managing different trust sources to achieve cross-domain trust and configurable trust-policy. To integrate EAS with e-business systems agilely, we put forward an agile integrating solution utilizing technologies such as Web service and remote scripting.
Evaluation of a Hemostasis Model for Teaching Basic Surgical Skills (1996)

The need for alternative methods of teaching veterinary medicine and surgery has increased in recent years because of increasing costs and changing public opinion. For these reasons a hemostasis model was developed that mimics the arteries and veins of the peripheral vascular system, and can be used to teach the basic skills involved in blood vessel ligation and division. This study evaluated the effectiveness of the fluid hemostasis model compared with using live animals for teaching these skills. Forty sophomore veterinary students participated in the study. Two groups of 20 students each received identical instruction in the basic techniques required for vessel ligation and division. The students then completed various exercises using inanimate models to objectively evaluate their psychomotor skills. Both groups then practiced the techniques for equal time periods; one group used the hemostasis model and the other performed a splenectomy on live dogs. After the practice session, the students were videotaped (for later evaluation), as they performed vessel ligations and divisions. The students then repeated the exercises using the inanimate models for evaluation of skills improvement. Questionnaire responses before and after the project were obtained to determine the students' views on the need for inanimate models for teaching purposes. Results of this study indicate that the hemostasis model was as effective as live animals for teaching the basic skills involved in blood vessel ligation. The students' opinions regarding the use of properly designed inanimate models for teaching these skills were dramatically changed.
Negative-bias-temperature-instability and hot carrier effects in nanowire junctionless p-channel multigate transistors (2012)

Negative-bias-temperature-instability (NBTI) and hot-carrier induced device degradation have been experimentally compared between accumulation mode (AM) p-channel multigate transistors (pMuGFETs) and junctionless (JL) pMuGFET. NBTI degradation is less significant in junctionless pMuGFETs than AM pMuGFETs. The threshold voltage shift is less significant in junctionless transistors than AM transistors. The device simulation shows that the peak of lateral electric field and the impact ionization rate of AM device are larger than those of junctionless devices.
Characterization of Three-Dimensional Retinal Tissue Derived from Human Embryonic Stem Cells in Adherent Monolayer Cultures (2015)

Stem cell-based therapy of retinal degenerative conditions is a promising modality to treat blindness, but requires new strategies to improve the number of functionally integrating cells. Grafting semidifferentiated retinal tissue rather than progenitors allows preservation of tissue structure and connectivity in retinal grafts, mandatory for vision restoration. Using human embryonic stem cells (hESCs), we derived retinal tissue growing in adherent conditions consisting of conjoined neural retina and retinal pigment epithelial (RPE) cells and evaluated cell fate determination and maturation in this tissue. We found that deriving such tissue in adherent conditions robustly induces all eye field genes (RX, PAX6, LHX2, SIX3, SIX6) and produces four layers of pure populations of retinal cells: RPE (expressing NHERF1, EZRIN, RPE65, DCT, TYR, TYRP, MITF, PMEL), early photoreceptors (PRs) (coexpressing CRX and RCVRN), inner nuclear layer neurons (expressing CALB2), and retinal ganglion cells [RGCs, expressing BRN3B and Neurofilament (NF) 200]. Furthermore, we found that retinal progenitors divide at the apical side of the hESC-derived retinal tissue (next to the RPE layer) and then migrate toward the basal side, similar to that found during embryonic retinogenesis. We detected synaptogenesis in hESC-derived retinal tissue, and found neurons containing many synaptophysin-positive boutons within the RGC and PR layers. We also observed long NF200-positive axons projected by RGCs toward the apical side. Whole-cell recordings demonstrated that putative amacrine and/or ganglion cells exhibited electrophysiological responses reminiscent of those in normal retinal neurons. These responses included voltage-gated Na(+) and K(+) currents, depolarization-induced spiking, and responses to neurotransmitter receptor agonists. Differentiation in adherent conditions allows generation of long and flexible pieces of 3D retinal tissue suitable for isolating transplantable slices of tissue for retinal replacement therapies.
Determination of graphene work function and graphene-insulator-semiconductor band alignment by internal photoemission spectroscopy (2012)

We determined the band alignment of a graphene-insulator-semiconductor structure using internal photoemission spectroscopy. From the flatband voltage and Dirac voltage, we infer a 4.6× 1011cm−2 negative extrinsic charge present on the graphene surface. Also, we extract the graphene work function to be 4.56 eV, in excellent agreement with theoretical and experimental values in literature. Electron and hole injection from heavily doped p-type silicon (Si) are both observed. The barrier height from the top of the valence band of Si to the bottom of the conduction band of silicon dioxide (SiO2) is found to be 4.3 eV. The small optical absorption in graphene makes it a good transparent contact to enable the direct observation of hole injection from Si to graphene. The barrier height for holes escaping from the bottom of Si conduction band to the top of SiO2 valence band is found to be 4.6 eV.
Ring modulator small-signal response analysis based on pole-zero representation (2016)

We present a closed-form expression for the small-signal response of a depletion-mode ring modulator and verify it by measurement results. Both electrical and optical behavior of micro-ring modulator as well as the loss variation due to the index modulation is considered in the derivation. This expression suggests that a ring modulator is a third-order system with one real pole, one zero and a pair of complex-conjugate poles. The exact positions of the poles/zero are given and shown to be dependent upon parameters such as electrical bandwidth, coupling condition, optical loss, and sign/value of laser detunings. We show that the location of zero is different for positive and negative detuning, and therefore, the ring modulator frequency response is asymmetric. We use the gain-bandwidth product as a figure of merit and calculate it for various pole/zero locations. We show that gain-bandwidth for the over-coupled ring modulator is superior compared to other coupling conditions. Also, we show that the gain-bandwidth product can be increased to a limit by increasing the electrical bandwidth.
Preparation of Omega‐3 PUFA Concentrates from Fish Oils via Urea Complexation (1988)

Abstract The effect of weight ratio of urea to fatty acids and the urea‐fatty acid adduct crystallization temperature on the enrichment of eicosapentaenoic acid from marine oil fatty acids was studied. The optimum ratio of urea to fatty acids was found to be 3 : 1 for laboratory scale preparations and the optimum temperature for the formation of urea‐fatty acid adduct was 1°C. At very low temperatures (−12, −18, −35°C) the recovery efficiency for EPA was reduced. Using these optimum values, enrichment of EPA and other n‐3 polyunsaturated fatty acids via urea complexation was carried out on a pilot plant scale in a variety of North Atlantic and North Pacific fist oils and a seal oil. Irrespective of hte type of starting oil, all the oils gave a concentrate with 69–85% total n‐3 PUFA with an overall yield of 17–20%. Menhaden is clearly an ideal oil for preparation of EPA concentrate, as the starting oil usually has a higher proportion of EPA to DHA than most of the other commercial fish oils.
Radiolabelled Peptide Ergot Alkaloids. 83. Mitteilung über Mutterkornalkaloide (1976)

Abstract Paspalic acid ( 11 ), labelled at different positions with 3 H or 14 C and at specific activities up to 1 Ci/mmol ( 3 H) and 2mCi/mmol ( 14 C), has been prepared biosynthetically in a scale of 2–5 mmol in submerged cultures of a selected strain of Claviceps paspali by incorporation of DL ‐[5‐ 3 H]‐tryptophan, DL ‐[6‐ 3 H]‐tryptophan, DL ‐[alanine‐2,3‐ 3 H]‐tryptophan, or DL ‐[alanine‐3‐ 14 C]‐tryptophan. Radioactive lysergic acid ( 12 ) was obtained from paspalic acid by base catalysed rearrangement. The procedures for labelling the precursors at high specific activity arc described. The ergolene carboxylic acids 11 and 12 were used as key intermediates for tlie chemical synthesis of radiolabelled peptide ergot alkaloids 14 required for pharinacokinetic and metabolic studies. Linking of the aminocyclols 13 (peptide parts) of the ergotarnine (R 1 = methyl), ergoxine (R 1 = ethyl) and ergotoxine (R 1 =isopropyl) series with a reactive derivative of either lysergic acid or its mixture with paspalic acid was accomplished by standard procedures. l‐Mctliyl‐[13‐ 3 H]‐ergotarnine (MY 25) ( 16 ) and 2‐brotno‐α‐ergocryptine (bromocriptine, C73 154) ( 17 ), labelled with 3 H at position 12 and with 14 C in position 4, were obtained by alkylating 3 H‐ergolamine and by ljrominating appropriately labelled α‐ergocryptines. Radioactive peptide ergot alltaloitls labelled by tlic present nietliotl proved suitable for the use in biological tracer studies.
TRAF6 ubiquitinates TGFβ type I receptor to promote its cleavage and nuclear translocation in cancer (2011)

Transforming growth factor β (TGFβ) is a pluripotent cytokine promoting epithelial cell plasticity during morphogenesis and tumour progression. TGFβ binding to type II and type I serine/threonine kinase receptors (TβRII and TβRI) causes activation of different intracellular signaling pathways. TβRI is associated with the ubiquitin ligase tumor necrosis factor receptor (TNFR)-associated factor 6 (TRAF6). Here we show that TGFβ, via TRAF6, causes Lys63-linked polyubiquitination of TβRI, promoting cleavage of TβRI by TNF-alpha converting enzyme (TACE), in a PKCζ-dependent manner. The liberated intracellular domain (ICD) of TβRI associates with the transcriptional regulator p300 to activate genes involved in tumour cell invasiveness, such as Snail and MMP2. Moreover, TGFβ-induced invasion of cancer cells is TACE- and PKCζ- dependent and the TβRI ICD is localized in the nuclei of different kinds of tumour cells in tissue sections. Thus, our data reveal a specific role for TβRI in TGFβ mediated tumour invasion.
Web-based performance visualization of distributed discrete event simulation (1999)

Article Free Access Share on Web-based performance visualization of distributed discrete event simulation Authors: Adel S. Elmaghraby Speed School, Computer Engineering and Computer Science Dept., University of Louisville, Louisville, KY Speed School, Computer Engineering and Computer Science Dept., University of Louisville, Louisville, KYView Profile , Sherif A. Elfayoumy Speed School, Computer Engineering and Computer Science Dept., University of Louisville, Louisville, KY Speed School, Computer Engineering and Computer Science Dept., University of Louisville, Louisville, KYView Profile , Irfan S. Karachiwala Speed School, Computer Engineering and Computer Science Dept., University of Louisville, Louisville, KY Speed School, Computer Engineering and Computer Science Dept., University of Louisville, Louisville, KYView Profile , James H. Graham Speed School, Computer Engineering and Computer Science Dept., University of Louisville, Louisville, KY Speed School, Computer Engineering and Computer Science Dept., University of Louisville, Louisville, KYView Profile , Ahmed Z. Emam Speed School, Computer Engineering and Computer Science Dept., University of Louisville, Louisville, KY Speed School, Computer Engineering and Computer Science Dept., University of Louisville, Louisville, KYView Profile , AlaaEldin Sleem Speed School, Computer Engineering and Computer Science Dept., University of Louisville, Louisville, KY Speed School, Computer Engineering and Computer Science Dept., University of Louisville, Louisville, KYView Profile Authors Info & Claims WSC '99: Proceedings of the 31st conference on Winter simulation: Simulation---a bridge to the future - Volume 2December 1999 Pages 1618–1623https://doi.org/10.1145/324898.325346Published:01 December 1999Publication History 1citation271DownloadsMetricsTotal Citations1Total Downloads271Last 12 Months7Last 6 weeks2 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
Analysis of the phenotypic abnormalities in lymphoedema-distichiasis syndrome in 74 patients with <i>FOXC2</i> mutations or linkage to 16q24 (2002)

Unlike previous publications, the thorough clinical characterisation of our patients permits more accurate prediction of various phenotypic abnormalities likely to manifest in subjects with FOXC2 mutations.
Lightning Protection of Wind Turbines (2010)

Wind turbines are the largest contributor to renewa<br/>ble energy both in Britain and the<br/>rest of Europe. With a rise in the installed capaci<br/>ty and an increase in offshore wind<br/>energy due to governments green targets by 2020, th<br/>ere has been a large<br/>development in new wind turbines for optimized perf<br/>ormance. The present thesis deals<br/>with the uncertainties in regards to the lightning<br/>phenomenon on wind turbines with<br/>emphasis on the rotor blades. Rotor blades are the<br/>most expensive part to replace in<br/>the event of lightning related damage. The research<br/>presents results based on lightning<br/>data analysis on wind turbines, backed up by finite<br/>element analysis testing of wind<br/>turbine systems. The final chapters include the tes<br/>ting and improving of lightning<br/>protection systems installed on modern day rotor bl<br/>ades.<br/>The first part of the thesis deals with the theoret<br/>ical understanding of the lightning<br/>phenomenon and its effect on wind turbine systems.<br/>The core work of the research<br/>begins with the analysis of lightning data collecte<br/>d over Nysted wind farm and different<br/>wind turbines installed over the world. The data an<br/>alysis helps in identifying the parts of<br/>the wind turbine that are at high risk to lightning<br/>attachment and related damage. The<br/>peak current levels of the lightning strikes seen o<br/>n the wind turbine are compared with<br/>those in modern day lightning standards, and show t<br/>hat historic data in the standards<br/>are not an exact match to the real case scenarios.<br/>The lightning data analysis also<br/>sheds light into the importance of upward initiated<br/>lightning, which will become<br/>important for large wind turbines, especially in th<br/>eir new offshore environment. A full<br/>scale 3D FEA model of a wind turbine, with lightnin<br/>g protection systems installed in its<br/>rotor blades, is subjected to electrical stresses t<br/>o find likely attachment points in<br/>regards to upward initiated lightning, and these re<br/>sults are later compared to those<br/>found in the data analysis.<br/>The second half of the thesis deals with the testin<br/>g of new materials and prototype<br/>blades, to be introduced to reduce their radar cros<br/>s section. The new materials include<br/>a large amount of carbon content which affects the<br/>efficiency of the lightning protection<br/>system. High voltage and high current tests backed<br/>up with finite element analysis<br/>have been performed to find how these new materials<br/>affect the performance of the<br/>lightning protection system. The results indicate t<br/>hat further work needs to be done<br/>before these new materials can be integrated into t<br/>he blade, as they increase the risk<br/>of lightning related damage to the blade.
An anionic organic mixed-valence system with a remarkably well-resolved vibrational structure in its intervalence band (2002)

The reduction of a phenylene-bridged bis(dioxaborine) affords a strongly delocalised organic mixed-valence system; for the first time details of the vibrations coupled to the electron transfer have been extracted from the intervalence band.
Polymer-assisted, multi-step solution phase synthesis and biological screening of histone deacetylase inhibitors (2004)

The polymer-assisted solution phase synthesis (PASP) of an array of histone deacetylase (HDAc) inhibitors is described. HDAc inhibitors have considerable potential as new anti-proliferative agents. Selected compounds were shown to inhibit both human endothelial cell proliferation, and the formation of tubules (neovascularisation) in an in vitro model of angiogenesis.
Materials for organic solar cells: the C<sub>60</sub>/π-conjugated oligomer approach (2004)

This tutorial review surveys recent advances in the field of C60/pi-conjugated oligomer donor-acceptor ensembles. In particular, different synthetic strategies are discussed that were developed to link pi-conjugated oligomers, as versatile photoexcited state electron donors, to C60. We highlight relationships between the nature/structural aspects of pi-conjugated donor systems and a variety of physico-chemical features. Modifications of the oligomeric components are discussed under aspects of tailoring (i) the absorption cross-section of the chromophore in the visible region, (ii) the oxidation potential of the oligomeric donor moiety, (iii) the size, shape, or chemical makeup of the oligomer, and (iv) the stabilization of the charge-separated radical ion pairs. In the final section, the applicability of selected materials for the fabrication of photovoltaic devices is analyzed.