gebhart/patrepeval huggingface dataset:
<title>Single-analyte to multianalyte fluorescence sensors</title> (1999)

The rational design of small molecules for the selective complexation of analytes has reached a level of sophistication such that there exists a high degree of prediction. An effective strategy for transforming these hosts into sensors involves covalently attaching a fluorophore to the receptor which displays some fluorescence modulation when analyte is bound. Competition methods, such as those used with antibodies, are also amenable to these synthetic receptors, yet there are few examples. In our laboratories, the use of common dyes in competition assays with small molecules has proven very effective. For example, an assay for citrate in beverages and an assay for the secondary messenger IP<SUB>3</SUB> in cells have been developed. Another approach we have explored focuses on multi-analyte sensor arrays with attempt to mimic the mammalian sense of taste. Our system utilizes polymer resin beads with the desired sensors covalently attached. These functionalized microspheres are then immobilized into micromachined wells on a silicon chip thereby creating our taste buds. Exposure of the resin to analyte causes a change in the transmittance of the bead. This change can be fluorescent or colorimetric. Optical interrogation of the microspheres, by illuminating from one side of the wafer and collecting the signal on the other, results in an image. These data streams are collected using a CCD camera which creates red, green and blue (RGB) patterns that are distinct and reproducible for their environments. Analysis of this data can identify and quantify the analytes present.
Controlling plate vibrations using piezoelectric actuators (1994)

Launch vehicles often experience severe vibration problems during the ascent phase due to intense acoustic fields and mechanically transmitted disturbances. These disturbances are characterized by a large number of resonances over a wide-band frequency spectrum. The payloads inside the launch vehicle must be designed strong enough to withstand these structural vibrations. Passive isolators are used to isolate the payloads from the vibrating structure but this type of isolation has no effect on the acoustic excitations. This study focuses on methods for achieving active damping on plate structures by means of piezoelectric actuators (PZTs). An experiment was conducted using PZT actuators and accelerometers mounted on a rectangular plate with an active damping controller implemented on a 486 computer. Two control design methods were evaluated for this application: the H/sub /spl infin method and rate feedback.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Stored luminescence computed tomography (2014)

Phosphor nanoparticles made of doped semiconductors and pre-excited by x-ray radiation were recently reported for their luminescence emission in the range of 650-770 nm upon near-infrared (NIR) light stimulation. These nanophosphors can be functionalized as optical probes for molecular imaging. In this paper, we present stored luminescence computed tomography to reconstruct a nanophosphor distribution in an object. The propagation of x rays in a biological object allows significantly better localization and deeper penetration. Moreover, the nanophosphors, which are pre-excited with collimated x-ray beams or focused x-ray waves, can be successively stimulated for stored luminescence emissions by variable NIR stimulation patterns. The sequentially detected luminescence signals provide more information of a nanophosphor spatial distribution for more accurate image reconstruction and higher image resolution. A realistic numerical study is performed to demonstrate the feasibility and merits of the proposed approach.
A permanent-magnet rotor containing an electrical winding to improve detection of rotor angular position (1999)

Sensorless detection of rotor angular position is desirable for decreasing the cost and increasing the reliability of permanent magnet AC motor drive systems. This paper describes tests performed on a rotor that has surface-mounted magnets and a conducting winding that links only the d axis of the rotor. The rotor winding increases the variation of the stator winding inductance that occurs as the rotor rotates and improves the accuracy of rotor angular position estimates.
Multiaxis Three-Dimensional Flat Woven Preforms - Tube Carrier Weaving (2009)

A multiaxis three-dimensional (3D) flat woven preform and a weaving method have been developed. The structure has five yarn sets, which are bias(+) and bias(—), warp, filling and Z-yarns. The weaving method has been introduced to form the structure. An experimental prototype was constructed to trial the method. In this technique, called “tube-carrier weaving”, bias(+) and bias(—) yarns are closely laid all over the surface. The preliminary study shows that the structure and weaving method seem feasible under the test conditions.
Does internet media traffic really follow Zipf-like distribution? (2007)

No abstract available.
Fiber reinforced piezoelectric bender transducer (1983)

Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter Facebook Reddit LinkedIn Tools Icon Tools Reprints and Permissions Cite Icon Cite Search Site Citation Tomosaburo Kitamura, Kosuke Miyahara, Yoichi Kodera; Fiber reinforced piezoelectric bender transducer. J. Acoust. Soc. Am. 1 July 1983; 74 (1): 388. https://doi.org/10.1121/1.389610 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAcoustical Society of AmericaThe Journal of the Acoustical Society of America Search Advanced Search |Citation Search
The Kinetics of Sulphur Sensitization: Sulphide Migration During Digestion (1972)

The sensitization and latent fogging of a photoemulsion with several sulphur sensitizers was followed. The kinetic law of sulphur sensitization observed was 1/t =cQm where t is digestion time, Q is sensitizer quantity, and c and m are constants depending on the sensitizer used and the emulsion conditions. These results, in conjunction with the known kinetics of sulphide formation are not consistent, with a single reaction step to give a sulphide sensitivity speck. A two-step reaction where the second step is not first order may be involved, and this may be the production of adsorbed sulphide atoms followed by their rearrangement into specks. A convenient experimental technique is described for following the photographic sensitometric properties of a digesting silver halide photoemulsion by taking a large number of Samples.
Whole‐cell biocatalytic and de novo production of alkanes from free fatty acids in <i>Saccharomyces cerevisiae</i> (2015)

Rapid global industrialization in the past decades has led to extensive utilization of fossil fuels, which resulted in pressing environmental problems due to excessive carbon emission. This prompted increasing interest in developing advanced biofuels with higher energy density to substitute fossil fuels and bio-alkane has gained attention as an ideal drop-in fuel candidate. Production of alkanes in bacteria has been widely studied but studies on the utilization of the robust yeast host, Saccharomyces cerevisiae, for alkane biosynthesis have been lacking. In this proof-of-principle study, we present the unprecedented engineering of S. cerevisiae for conversion of free fatty acids to alkanes. A fatty acid α-dioxygenase from Oryza sativa (rice) was expressed in S. cerevisiae to transform C<sub>12-18</sub> free fatty acids to C<sub>11-17</sub> aldehydes. Co-expression of a cyanobacterial aldehyde deformylating oxygenase converted the aldehydes to the desired alkanes. We demonstrated the versatility of the pathway by performing whole-cell biocatalytic conversion of exogenous free fatty acid feedstocks into alkanes as well as introducing the pathway into a free fatty acid overproducer for de novo production of alkanes from simple sugar. The results from this work are anticipated to advance the development of yeast hosts for alkane production. Biotechnol. Bioeng. 2017;114: 232-237. © 2016 The Authors. Biotechnology and Bioengineering Published by Wiley Periodicals, Inc.
Steady-State Investigation of Vapor Deposited Micro Heat Pipe Arrays (1995)

An experimental investigation of vapor deposited micro heat pipe arrays was conducted using arrays of 34 and 66 micro heat pipes occupying 0.75 and 1.45 percent of the cross-sectional area, respectively. The performance of wafers containing the arrays was compared with that of a plain silicon wafer. All of the wafers had 8 × 8 mm thermofoil heaters located on the bottom surface to simulate the active devices in an actual application. The temperature distributions across the wafers were obtained using a Hughes Probeye TVS Infrared Thermal Imaging System and a standard VHS video recorder. For wafers containing arrays of 34 vapor deposited micro heat pipes, the steady-state experimental data indicated a reduction in the maximum surface temperature and temperature gradients of 24.4 and 27.4 percent, respectively, coupled with an improvement in the effective thermal conductivity of 41.7 percent. For wafers containing arrays of 66 vapor deposited micro heat pipes, the corresponding reductions in the surface temperature and temperature gradients were 29.0 and 41.7 percent, respectively, and the effective thermal conductivity increased 47.1 percent, for input heat fluxes of 4.70 W/cm2. The experimental results were compared with the results of a previously developed numerical model, which was shown to predict the temperature distribution with a high degree of accuracy, for wafers both with and without the heat pipe arrays.
Broadband Rydberg atom based self-calibrating RF E-field probe (2014)

We present a significantly new approach for an electric (E) field probe design. The probe is based on the interaction of RF-fields with Rydberg atoms, where alkali atoms are excited optically to Rydberg states and the applied RF-field alters the resonant state of the atoms. For this probe, the Rydberg atoms are excited in a glass vapor cell. The Rydberg atoms act like an RF-to-optical transducer, converting an RF E-field to an optical-frequency response. The probe utilizes the concept of Electromagnetically Induced Transparency (EIT). The RF transition in the four-level atomic system causes a split of the EIT transmission spectrum for the probe laser. This splitting is easily measured and is directly proportional to the applied RF field amplitude. Therefore, by measuring this splitting we get a direct measurement of the RF E-field strength. The significant dipole response of Rydberg atoms over the GHz regime enables this technique to make traceable measurements over a large frequency band including 1-500 GHz. We will show that, with one probe, measurements can be made over a very large frequency range. This is a truly broadband probe/sensor. In this paper, we report on our results in the development of this probe.
Passive state preparation in continuous-variable quantum key distribution (2018)

We propose a passive continuous-variable quantum key distribution scheme, where Alice splits the output of a thermal source into two spatial modes, measures one locally and transmits the other mode to Bob after applying attenuation.
Molecular Modeling and Site-directed Mutagenesis Define the Catalytic Motif in Human γ-Glutamyl Hydrolase (2000)

Human gamma-glutamyl hydrolase (hGH) is a central enzyme in folyl and antifolylpoly-gamma-glutamate metabolism, which functions by catalyzing the cleavage of the gamma-glutamyl chain of substrates. We previously reported that Cys-110 is essential for activity. Using the sequence of hGH as a query, alignment searches of protein data bases were made using the SSearch and TPROBE programs. Significant similarity was found between hGH and the glutamine amidotransferase type I domain of Escherichia coli carbamoyl phosphate synthetase. The resulting hypothesis is that the catalytic fold of hGH is similar to the folding of this domain in carbamoyl phosphate synthetase. This model predicts that Cys-110 of hGH is the active site nucleophile and forms a catalytic triad with residues His-220 and Glu-222. The hGH mutants C110A, H220A, and E222A were prepared. Consistent with the model, mutants C110A and H220A were inactive. However, the V(max) of the E222A hGH mutant was reduced only 6-fold relative to the wild-type enzyme. The model also predicted that His-171 in hGH may be involved in substrate binding. The H171N hGH mutant was found to have a 250-fold reduced V(max). These studies to determine the catalytic mechanism begin to define the three dimensional interactions of hGH with poly-gamma-glutamate substrates.
Invited Article: A compact optically coherent fiber frequency comb (2015)

We describe the design, fabrication, and performance of a self-referenced, optically coherent frequency comb. The system robustness is derived from a combination of an optics package based on polarization-maintaining fiber, saturable absorbers for mode-locking, high signal-to-noise ratio (SNR) detection of the control signals, and digital feedback control for frequency stabilization. The output is phase-coherent over a 1-2 μm octave-spanning spectrum with a pulse repetition rate of ∼200 MHz and a residual pulse-to-pulse timing jitter <3 fs well within the requirements of most frequency-comb applications. Digital control enables phase coherent operation for over 90 h, critical for phase-sensitive applications such as timekeeping. We show that this phase-slip free operation follows the fundamental limit set by the SNR of the control signals. Performance metrics from three nearly identical combs are presented. This laptop-sized comb should enable a wide-range of applications beyond the laboratory.
Detection of Diverse Variants of Human Immunodeficiency Virus–1 Groups M, N, and O and Simian Immunodeficiency Viruses from Chimpanzees by Using Generic<i>pol</i>and<i>env</i>Primer Pairs (2000)

Human immunodeficiency virus type 1 (HIV-1) infection of humans is the result of independent cross-species transmissions of simian immunodeficiency viruses (SIVcpz) from naturally infected chimpanzees (Pan troglodytes troglodytes) to man. To develop a polymerase chain reaction-based assay capable of detecting members of all major phylogenetic SIVcpz and HIV-1 lineages (groups M, N, and O), primer pairs in conserved pol and env regions were designed. Both primer sets amplified </=10 copies of selected group M reference clones (subtypes A-H), proviral DNA or RNA of group N (YBF30), and group O of HIV-1 and also amplified divergent SIVcpz from cultured isolates (SIVcpzGAB1 and SIVcpzANT), uncultured spleen tissue (SIVcpzUS), and plasma (SIVcpzANT and SIVcpzUS). Sequences of the 2 amplicons (445 bp for gp41 and 261 bp for integrase) are of sufficient length for phylogenetic analyses, allowing both group and subtype classifications of the human viruses. Finally, both primer pairs are highly sensitive (>99%) in amplifying viral sequences from plasma taken from patients infected with HIV-1 group M (n=226) and O (n=17) viruses.
Development of the innovative GMA wire improving the flow direction of molten pool (2009)

As sulfur content increases in weld metals, hot cracking is likely to occur. Therefore, to prevent hot cracking, the sulfur content of the welding consumables is controlled to be as low as possible in general. On the other hand, sulfur can considerably reduce the surface tension of the molten iron. Utilizing the latter characteristics of sulfur, the authors have succeeded in the development of an innovative gas metal arc (GMA) welding wire that contains a higher amount of sulfur as compared with conventional wires and possesses the flowing characteristics of the molten pool more suitable for thin steel plate welding. It has also been found through this research that the hot crack resistance of the weld metal degraded by increasing sulfur can be canceled by decreasing the carbon content of the wire to a substantially low level. The newly developed wire offers the following advantages over conventional solid wires: (1) wider and more consistent bead shape, (2) less undercut at higher welding speeds, (3) easier setting of the welding conditions due to wider tolerability to voltage fluctuations, and (4) excellent paint adhesiveness due to less slag generation.
A GPU-based method in recovering the full 3D deformation field using multiple 2D fluoroscopic views in lung navigation (2016)

In this work, we present a GPU implementation for 2D-3D deformable registration to fully recover the 3D lung-deformation-field using multiple 2D fluoroscopic views. Intensive computational requirement for 2D-3D deformable registration prevents its clinical usage. To make our method clinically applicable, we developed a GPU kernel system to reduce the computation time from hours to seconds. The proposed kernel system employed a total of six GPU kernels. These six kernels serve the basis of two kernel pipelines: cost calculation and gradient calculation, the outputs of which are fed into a CPU-based optimizer to find the optimal deformation field. The evaluation was performed on both synthetic and real cases. The accuracy, evaluated using feature points, was 2.03mm on an average. A speedup factor of 690 could be reached over pure CPU-based implementation.
ChemInform Abstract: ANTIHYPERTENSIVE PYRROLO(1,2‐C)QUINAZOLINES AND PYRROLO(1,2‐C)QUINAZOLINONES (1982)

Abstract Die Methylchinazoline (I) kondensieren mit Brombrenztraubensäureethylester (II) zu den Pyrrolochinazolinen (III).
A 9-GS/s 1.125-GHz BW Oversampling Continuous-Time Pipeline ADC Achieving −164-dBFS/Hz NSD (2017)

An oversampled continuous-time (CT) pipeline ADC clocked at 9 GHz achieving 1.125-GHz bandwidth and -164 dBFS/Hz average small-signal noise density is presented. In contrast to traditional discrete-time (DT) pipeline ADCs, the system processes the signals in CT form throughout all the pipeline stages and thus sampling-induced artifacts such as aliasing and high-peak ADC driving current are mitigated. Despite the oversampled nature of the ADC, its digitization bandwidth is on par with that of traditional non-interleaved DT pipeline ADCs since CT signal processing is not constrained by settling time requirements. The ADC was fabricated in a 28-nm CMOS process technology and consumes 2.3 W.
Neuroleptic agents of the benzocycloheptapyridoisoquinoline series. 1. Syntheses and stereochemical and structural requirements for activity of butaclamol and related compounds (1975)

The syntheses and the structural and stereochemical requirements for antagonism of (+)-amphetamine-induced sterotypy are described for a series of benzocycloheptapyridoisoquinoline derivatives. One of these compounds, (plus or minus)-(4a,13b-trans)[3(OH),13b(H)-trans[-3-tert-butyl-2,3,4,4a,8,9,13b,14-octahydro-1H-benzo[6,7]cyclohepta[1,2,3-de]pyrido[2,1-a]isoquinolin-3ol hydrochloride (butaclamol hydrochloride, USAN), is currently being studied in man. The relationship between structure and antiamphetamine activity in this class of compounds is discussed.
Fabrication and Performance of Integrated Fluxgate for Current Sensing Applications (2017)

The recently developed Fluxgate technology from Texas Instruments has enabled the production of the industry's first current sensing integrated circuit with a fully integrated fluxgate device and a compensation coil driver. This paper presents an overview of the Fluxgate technology, focusing on the fabrication and performance of the integrated magnetic field sensing device.
Growth of 2 Inches 6H-SiC Single Crystals by Sublimation Method: The Comparison of α- and ß-SiC Powders (2005)

We examined the formation of poly-crystals and polytypes under the point of view applying various powder phases. 6H-SiC single crystal was easily grown by using the green (α-SiC) powder, while the poly-crystals were generated when β-SiC powder was utilized. The method of mixed β-SiC and carbon powder and of graphite pipe inserted in β-SiC powder were applied to overcome the generation of poly-crystals, respectively. It was confirmed that the occurrence of poly-crystals in 6H-SiC crystal was successfully suppressed by C-rich environment.
Abstract 953: Mechanistic rationale for inhibition of Poly(ADP-Ribose) Polymerase in ETS gene fusion positive prostate cancer (2011)

Abstract Recurrent fusions of ETS genes are considered driving mutations in a diverse array of cancers including Ewing's sarcoma, acute myeloid leukemia, and epithelial tumors such as prostate cancer. However, transcription factors like the ETS genes have been notoriously difficult to target therapeutically. In fact, while approximately 50% of all prostate cancers harbor ETS gene fusions, the most common variant fuses an androgen regulated promoter and the 5’-UTR of TMPRSS2 to the second exon of ERG resulting in the pathogenic overexpression of a slightly truncated ERG transcription factor. Here, we use IP-mass spectrometry to characterize the ETS protein interactome in prostate cancer. We show that the TMPRSS2:ERG gene fusion product interacts with the enzymes poly(ADP-ribose)polymerase 1 (PARP1) and the catalytic subunit of DNA protein kinase (DNA-PKcs) in a DNA-independent manner in both prostate cancer cells and tissues. ETS gene fusion-mediated transcription of several target genes including the invasion associated gene EZH2 requires both PARP1 and DNA-PKcs expression and activity. Likewise, cell invasion driven by ETS gene overexpression is inhibited by small molecule inhibitors or siRNA against these enzymes in matrigel coated transwell invasion assays (in vitro) as well as chicken chorioallantoic membrane intravasation and metastasis assays (in vivo). Importantly, pharmacological inhibition of PARP1 selectively inhibited the growth of 4 ETS positive, but not 5 ETS negative, prostate cancer cell xenografts. This analysis includes several prostate cancer cell lines, an isogenic model of hormone refractory prostate cancer and primary human tumors that were serially grown in mice. Finally, we find that TMPRSS2:ERG gene fusion overexpression leads to increased DNA double strand breaks as assessed by gamma-H2A.X staining and COMET assays. This DNA damage is then potentiated by PARP1 inhibition in a manner similar to that of BRCA1/2-deficiency.Thus, we propose that the ETS:PARP1 interaction axis may represent a novel target for therapeutic intervention in cancers with ETS gene fusions and that future clinical trials will help determine if this subgroup of patients preferentially benefits from the addition of PARP inhibitor therapy. Moreover, our study suggests that inhibition of co-factors necessary for function may represent a new paradigm of treatment for malignancies driven by oncogenic transcription factors. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 953. doi:10.1158/1538-7445.AM2011-953
The Prismatic Array Antenna a non Dispersive Travelling Wave Array Antenna (1980)

The prismatic array antenna is a new array antenna, the maximum gain direction of which is almost stationnary in the frequency band. A first scattering array acts as a feed for a second array tilted relative to the first one at some specific angle. Between the two arrays, propagation may be either a guided-space one (between parallel plates for example) or a free-space one (in case of two-dimensional arrays). This structure defines a non dispersive antenna, the "prismatic array antenna" in a frequency band of about 10%.