gebhart/patrepeval huggingface dataset:
Antimicrobial Resistance among Gram-Negative Bacilli Causing Infections in Intensive Care Unit Patients in the United States between 1993 and 2004 (2007)

During the 12-year period from 1993 to 2004, antimicrobial susceptibility profiles of 74,394 gram-negative bacillus isolates recovered from intensive care unit (ICU) patients in United States hospitals were determined by participating hospitals and collected in a central location. MICs for 12 different agents were determined using a standardized broth microdilution method. The 11 organisms most frequently isolated were Pseudomonas aeruginosa (22.2%), Escherichia coli (18.8%), Klebsiella pneumoniae (14.2%), Enterobacter cloacae (9.1%), Acinetobacter spp. (6.2%), Serratia marcescens (5.5%), Enterobacter aerogenes (4.4%), Stenotrophomonas maltophilia (4.3%), Proteus mirabilis (4.0%), Klebsiella oxytoca (2.7%), and Citrobacter freundii (2.0%). Specimen sources included the lower respiratory tract (52.1%), urine (17.3%), and blood (14.2%). Rates of resistance to many of the antibiotics tested remained stable during the 12-year study period. Carbapenems were the most active drugs tested against most of the bacterial species. E. coli and P. mirabilis remained susceptible to most of the drugs tested. Mean rates of resistance to 9 of the 12 drugs tested increased with Acinetobacter spp. Rates of resistance to ciprofloxacin increased over the study period for most species. Ceftazidime was the only agent to which a number of species (Acinetobacter spp., C. freundii, E. aerogenes, K. pneumoniae, P. aeruginosa, and S. marcescens) became more susceptible. The prevalence of multidrug resistance, defined as resistance to at least one extended-spectrum cephalosporin, one aminoglycoside, and ciprofloxacin, increased substantially among ICU isolates of Acinetobacter spp., P. aeruginosa, K. pneumoniae, and E. cloacae.
Growth of nanotubes and chemical sensor applications (2004)

We have used a number of methods to grow long aligned single-walled carbon nanotubes. Geometries include individual long tubes, dense parallel arrays, and long freely suspended nanotubes. We have fabricated a variety of devices for applications such as multiprobe resistance measurement and high-current field effect transistors. In addition, we have measured conductance of single-walled semiconducting carbon nanotubes in field-effect transistor geometry and investigated the device response to water and alcoholic vapors. We observe significant changes in FET drain current when the device is exposed to various kinds of different solvent. These responses are reversible and reproducible over many cycles of vapor exposure. Our experiments demonstrate that carbon nanotube FETs are sensitive to a wide range of solvent vapors at concentrations in the ppm range.
High‐efficiency genome editing using a <i>dmc1</i> promoter‐controlled <scp>CRISPR</scp>/Cas9 system in maize (2018)

Previous studies revealed that the promoters for driving both Cas9 and sgRNAs are quite important for efficient genome editing by CRISPR/Cas9 in plants. Here, we report our results of targeted genome editing using the maize dmc1 gene promoter combined with the U3 promoter for Cas9 and sgRNA, respectively. Three loci in the maize genome were selected for targeting. The T0 plants regenerated were highly efficiently edited at the target sites with homozygous or bi-allelic mutants accounting for about 66%. The mutations in T0 plants could be stably transmitted to the T1 generation, and new mutations could be generated in gametes or zygotes. Whole-genome resequencing indicated that no off-target mutations could be detected in the predicted loci with sequence similarity to the targeted site. Our results show that the dmc1 promoter-controlled (DPC) CRISPR/Cas9 system is highly efficient in maize and provide further evidence that the optimization of the promoters used for the CRISPR/Cas9 system is important for enhancing the efficiency of targeted genome editing in plants. The evolutionary conservation of the dmc1 gene suggests its potential for use in other plant species.
Effect of Feed Additives on Productivity and Campylobacter spp. Loads in Broilers Reared under Free Range Conditions (2017)

The poultry reservoir, especially broiler meat, is generally recognized as one of the most-important sources for human Campylobacteriosis. The measures to control <i>Campylobacter</i> targeted essentially the primary production level. The aim of this work was to evaluate the effectiveness of different treatments against natural <i>Campylobacter</i> colonization in a French experimental farm of free-range broilers during the whole rearing period. Five commercial products and a combination of two of them were tested and all the products were added to feed or to water at the dose recommended by the suppliers. <i>Campylobacter</i> loads in caeca and on carcasses of broilers at the slaughter were determined by culture methods. Natural contamination of the flock occurred at the end of the indoor rearing period between day 35 and day 42. At day 42, the multispecies probiotic added to the feed reduced the contamination of 0.55 log<sub>10</sub> CFU/g (<i>p</i> = 0.02) but was not significant (<i>p</i> > 0.05) at the end of rearing at day 78. However, another treatment, a combination of a cation exchange clay-based product in feed and an organic acid mixture (formic acid, sodium formate, lactic acid, propionic acid) in water, led to a slight but significant reduction of 0.82 ± 0.25 log<sub>10</sub> CFU/g (<i>p</i> = 0.02) compared to the control group at day 78. Testing this combination in field conditions in several flocks is needed to determine if it is biologically relevant and if it could be a valuable measure to reduce <i>Campylobacter</i> in broiler flocks.
Denial of Service Resistant Intrusion Detection Architecture (2000)

As the capabilities of intrusion detection systems (IDSs) advance, attackers may disable organizations&apos; IDSs before attempting to penetrate more valuable targets. To counter this threat, we present an IDS architecture that is resistant to denial-of-service (DOS) attacks. The architecture frustrates attackers by making IDS components invisible to attackers&apos; normal means of &quot;seeing&quot; in a network. Upon a successful attack, the architecture allows IDS components to relocate from attacked hosts to operational hosts thereby mitigating the attack. These capabilities are obtained by using mobile agent technology, utilizing network topology features, and by restricting the communication allowed between different types of IDS components.
Robust adaptive Kalman filtering-based speech enhancement algorithm (2004)

The paper deals with the problem of speech enhancement when only a corrupted speech signal is available for processing. Kalman filtering is known as an effective speech enhancement technique, in which the speech signal is usually modeled as an autoregressive (AR) model and represented in the state-space domain. Various approaches based on the Kalman filter have been presented in the literature. They usually operate in two steps: first, additive noise and driving process statistics and speech model parameters are estimated and second, the speech signal is estimated by using Kalman filtering. In the paper, sequential estimators are used for suboptimal adaptive estimation of the unknown a priori driving process and of additive noise statistics simultaneously with the system state. The estimation of time-varying AR signal model is based on a robust recursive least-square algorithm with variable forgetting factor. The proposed algorithm provides improved state estimates at little computational expense.
Rate and Concentration Dependence of Parathyroid Hormone Dynamics during Stepwise Changes in Serum Ionized Calcium in Normal Humans* (1990)

The relationship between circulating levels of PTH and the concentration and rate of change of ionized calcium (CaI) was studied in normal humans by measuring intact PTH during stepwise changes in CaI. Six normal subjects received two different citrate infusion protocols that produced stepwise decreases in CaI; one infusion produced a rapid decrement in calcium, and a second infusion produced a slower approach to the same (approximately 0.05 mmol/L) decline in calcium for each of four steps. The rapid decline in CaI resulted in a more marked increase in levels of PTH, which subsequently fell to levels similar to those with the slower infusion. For similar absolute changes in calcium, the mean maximal increment in PTH levels was significantly greater with the rapid infusion (36.4 +/- 3.1 ng/L) than with the slower infusion (19.4 +/- 2.1 ng/L; P = 0.001). Six additional subjects received infusions of citrate and calcium in a stepwise manner to induce either decreases or increases in CaI, followed by a return to baseline. During induced hypocalcemia, when calcium was changing slowly or not at all (i.e. at the plateau of each calcium change) PTH levels were not affected by the direction of change in calcium and appeared to be dependent upon the calcium concentration per se. At elevated levels of CaI, the PTH response to a stepwise decrease in calcium was blunted over that seen when CaI declined to or below baseline. Thus, the relationship between CaI and levels of PTH is dependent not only on the concentration but also on the rate of change in calcium, particularly during induced hypocalcemia; different rates of change in calcium result in different inverse sigmoidal relationships between PTH and CaI. When calcium is changing slowly or not at all, however, PTH levels appear to be dependent on the calcium concentration per se and are not affected by the previous direction or rate of change. Therefore, the role of the extracellular calcium concentration in the control of PTH secretion is part of more complex and dynamic regulatory mechanisms.
3DCLIMBER: A climbing robot for inspection of 3D human made structures (2008)

3DCLIMBER is a running project in the University of Coimbra for developing a climbing robot with the capability of manipulating over 3D human-made structures. This paper mainly discuss the conceptual and detailed design and development of a Pole Climbing robot with minimum degrees of freedom which can climb over 3D structures with bends and branches followed by Preliminary test results of the robot performance. Electronics architecture and control algorithms are briefly described. The paper finishes with discussion of the current results and identifies some future works.
Characterisation of the capsid protein gene from a nodavirus strain affecting the Atlantic halibut Hippoglossus hippoglossus and design of an optimal reverse-transcriptase polymerase chain reaction (RT-PCR) detection assay (2000)

A 1349 nucleotide fragment of the RNA2 from a nodavirus affecting Atlantic halibut Hippoglossus hippoglossus was characterised and the nuclotide sequence (accession no. AJ245641) was employed to develop an optimal reverse-transcriptase polymerase chain reaction (RT-PCR) detection assay. The sequenced part of the RNA2 of Atlantic halibut nodavirus (strain AH95NorA) was highly similar in organisation to that of the RNA2 of striped jack nervous necrosis virus (SJNNV), and comprised features common to all nodaviruses. These characteristics confirmed that the virus that causes viral encephalopathy and retinopathy (VER) in Atlantic halibut is a nodavirus. The nucleotide sequence of the 1349 nucleotide fragment of Atlantic halibut nodavirus RNA2 was 80% identical to the RNA2 of SJNNV. The T2 region (830 nucleotides) of the RNA2 of Atlantic halibut nodavirus shared 98% of the nucleotide sequence when compared with the homologous region of barfin flounder nervous necrosis virus (BFNNV), while the nucleotide sequence identity to SJNNV in this region was 76%. Phylogenetic analysis based on the nucleotide sequences of the T4 region (421 nucleotides) of Atlantic halibut nodavirus and of other fish nodaviruses revealed a close relationship to the nodaviruses of the barfin flounder clad that have been found in other cold-water species (Pacific cod Gadus macrocephalus and barfin founder Verasper moseri). The nucleotide sequence of the RNA2 of Atlantic halibut nodavirus included some features that differ from that of SJNNV. The ORF of the RNA2 of Atlantic halibut nodavirus lacked 6 nucleotides through a single deletion and a 5-nucleotide deletion, separated by 4 nucleotides. The 3'-non-encoding region contained a 21 nucleotide insert and a 3 nucleotide deletion when compared with SJNNV. In comparison with the RNA2 of SJNNV, the 3'-non-encoding region showed a nucleotide sequence identity of 84.5%. A primer set based on the Atlantic halibut nodavirus nucleotide sequence was employed in order to design an optimal RT-PCR. The detection limit of the PCR was 10 to 100 copies of plasmid, while the detection limit of the RT-PCR assay was 100 to 1000 copies of in vitro transcribed viral RNA.
DESENSITIZATION OF G PROTEIN–COUPLED RECEPTORS AND NEURONAL FUNCTIONS (2004)

G protein-coupled receptors (GPCRs) have proven to be the most highly favorable class of drug targets in modern pharmacology. Over 90% of nonsensory GPCRs are expressed in the brain, where they play important roles in numerous neuronal functions. GPCRs can be desensitized following activation by agonists by becoming phosphorylated by members of the family of G protein-coupled receptor kinases (GRKs). Phosphorylated receptors are then bound by arrestins, which prevent further stimulation of G proteins and downstream signaling pathways. Discussed in this review are recent progress in understanding basics of GPCR desensitization, novel functional roles, patterns of brain expression, and receptor specificity of GRKs and beta arrestins in major brain functions. In particular, screening of genetically modified mice lacking individual GRKs or beta arrestins for alterations in behavioral and biochemical responses to cocaine and morphine has revealed a functional specificity in dopamine and mu-opioid receptor regulation of locomotion and analgesia. An important and specific role of GRKs and beta arrestins in regulating physiological responsiveness to psychostimulants and morphine suggests potential involvement of these molecules in certain brain disorders, such as addiction, Parkinson's disease, mood disorders, and schizophrenia. Furthermore, the utility of a pharmacological strategy aimed at targeting this GPCR desensitization machinery to regulate brain functions can be envisaged.
Mobility Spectrometer Studies on Hydrazine and Ammonia Detection (2011)

An airborne vapor analyzer for detecting sub- to low- parts-per-million (ppm) hydrazine in the presence of higher concentration levels of ammonia has been under development for the Orion program. The detector is based on ambient pressure ionization and ion mobility characterization. The detector encompasses: 1) a membrane inlet to exclude particulate and aerosols from the analyzer inlet; 2) a method to separate hydrazine from ammonia which would otherwise lead to loss of calibration and quantitative accuracy for the hydrazine determination; and 3) response and quantitative determinations for both hydrazine and ammonia. Laboratory studies were made to explore some of these features including mobility measurements mindful of power, size, and weight issues. The study recommended the use of a mobility spectrometer of traditional design with a reagent gas and equipped with an inlet transfer line of bonded phase fused silica tube. The inlet transfer line provided gas phase separation of neutrals of ammonia from hydrazine at 50 C simplifying significantly the ionization chemistry that underlies response in a mobility spectrometer. Performance of the analyzer was acceptable between ranges of 30 to 80 C for both the pre-fractionation column and the drift tube. An inlet comprised of a combined membrane with valve-less injector allowed high speed quantitative determination of ammonia and hydrazine without cross reactivity from common metabolites such as alcohols, esters, and aldehydes. Preliminary test results and some of the design features are discussed.
Toward global instantaneous decimeter-level positioning using tightly coupled multi-constellation and multi-frequency GNSS (2018)

Autonomous driving represents one of the emerging applications that require both high-precision positions and highly critical timeliness to reach stringent safety standards. We develop a method to potentially achieve global instantaneous decimeter-level positioning by virtue of tightly coupled multi-GNSS triple-frequency observations contributing to precise point positioning (PPP). Inter-system phase biases (ISPBs) for two wide-lane observables are first computed for each station to form inter-GNSS resolvable ambiguities, and then correspondingly two wide-lane fractional-cycle biases (FCBs) are computed for each satellite to recover the integer property of single-station ambiguities. With both ISPB and FCB products, we can accomplish tightly coupled multi-GNSS PPP wide-lane ambiguity resolution (PPP-WAR) using only a single epoch of triple-frequency observations on a global scale. To verify this method, we used 1 month of GPS/BeiDou/Galileo/QZSS data from 107 globally distributed stations and 1 h of such multi-GNSS data collected on a vehicle moving in an urban area. We found that both ISPB and FCB products could be estimated every 24 h with high precisions of around or below 0.1 cycles; 83–98% of their day-to-day variations fell within 0.1 cycles, facilitating their precise predictions for real-time applications. Using these corrections, we achieved both instantly and reliably ambiguity-fixed solutions at 91.2% of all epochs at the 107 stations on average; the resultant single-epoch positions reached a mean accuracy of 0.22 m, 0.18 m and 0.63 m for the east, north and up components, respectively, in case of abundant triple-frequency observations from over 15 satellites. Similarly, for the vehicle-borne test, we obtained instantaneous PPP-WAR solutions at 99.31% of all epochs and achieved a positioning accuracy of 0.29, 0.35 and 0.77 m for the east, north and up components, respectively, which improved significantly the identification of road lanes as opposed to other single-epoch solutions. Finally, we expect that the prospect of instantaneous PPP-WAR in aiding driverless vehicles can be more promising if, whenever possible, integrated with inertial sensors and/or smoothed through multi-epoch data.
How to monitor the protein cross-linking by formaldehyde, glutaraldehyde or glyoxal in cotton-seed protein protein-based films? (short communication) (1998)

Cotton-seed protein-based films can be formed from an aqueous solution by a casting process. Chemical cross-linking treatments of cotton-seed proteins by dialdehyde increased maximum puncture force and decreased solubility of films in water. Glutaraldehyde, formaldehyde and glyoxal are cross-linking agents capable of reacting with amino acid side chains, particularly with the lysine ENH2 group, to form Schiff bases. As a first approach, simple criteria (maximum puncture force, puncture deformation, and film solubility) were used to assess the efficiency of chemical treatments. In the present study the amount of lysine that did not react with cross-linking agents (reactive lysine) during cotton-seed protein film formation was used to assess the degree of protein cross-linking. We have used a new procedure that provide a quantitative determination of reactive lysine in films made from cotton-seed flour. Subsequently, this methodology was used to investigate the cross-linking of cotton-seed proteins by formaldehyde, glutaraldehyde or glyoxal in films and to analyse the relation between protein cross-linking and film maximum puncture force
Signature of the existence of the positronium molecule (1998)

The positronium molecule $({\mathrm{Ps}}_{2})$ has not been experimentally observed yet because its tiny (4.5 eV) binding energy cannot be detected when the molecule annihilates by emitting two photons with energy of 0.51 MeV each. It is shown in this paper that the electric dipole transition between the recently found $L=1$ excited state and the $L=0$ ground state with its characteristic photon energy of 4.94 eV is a clear signature of the existence of the positronium molecule and the possibility of its experimental observation is realistic. The probability of this transition is about 17% of the total decay rate. Another Coulomb four-body system containing positron HPs (the positronium hydride or hydrogen positride), is also included for comparison.
Fiber-reinforced concrete: an overview after 30 years of development (1997)

This paper presents a rhetorical discussion on the subject of fiber-reinforced concrete, FRC. It is intended as an overview of the types of commercially available FRCs and how they work. It discusses commonly applied terminology and models of mechanical behavior that form a basis for understanding material performance without presenting mathematical details. Historical review is intended to help build a background for what is currently understood about FRC rather than as historical reporting. References from both early and contemporary authors are included as a means of tying the subject together along a time line.
Task 38 - commercial mercury remediation demonstrations: Thermal retorting and physical separation/chemical leaching. Topical report, December 1, 1994--June 30, 1996 (1998)

Results are presented on the demonstration of two commercial technologies for the removal of mercury from soils found at natural gas metering sites. Technologies include a thermal retorting process and a combination of separation, leaching, and electrokinetic separation process.
Delayed treatment with chondroitinase ABC promotes sensorimotor recovery and plasticity after stroke in aged rats (2012)

Stroke is the dominant cause of sensorimotor disability that primarily affects the elderly. We now show that neuroplasticity and functional recovery after stroke is constrained by inhibitory chondroitin sulphates. In two blinded, randomized preclinical trials, degradation of chondroitin sulphate using chondroitinase ABC reactivated neuroplasticity and promoted sensorimotor recovery after stroke in elderly rats. Three days after stroke, chondroitinase ABC was microinjected into the cervical spinal cord to induce localized plasticity of forelimb sensorimotor spinal circuitry. Chondroitinase ABC effectively removed chondroitin sulphate from the extracellular matrix and perineuronal nets. Three different tests of sensorimotor function showed that chondroitinase ABC promoted recovery of forelimb function. Anterograde and retrograde tracing showed that chondroitinase ABC also induced sprouting of the contralesional corticospinal tract in the aged treated hemicord. Chondroitinase ABC did not neuroprotect the peri-infarct region. We show for the first time delayed chondroitinase ABC treatment promotes neuroanatomical and functional recovery after focal ischaemic stroke in an elderly nervous system.
The KCNQ2 potassium channel: splice variants, functional and developmental expression. Brain localization and comparison with KCNQ3 (1998)

Benign familial neonatal convulsions, an autosomal dominant epilepsy of newborns, are linked to mutations affecting two six-transmembrane potassium channels, KCNQ2 and KCNQ3. We isolated four splice variants of KCNQ2 in human brain. Two forms generate, after transient expression in COS cells, a potassium-selective current similar to the KCNQ1 current. L-735,821, a benzodiazepine molecule which inhibits the KCNQ1 channel activity (EC50 = 0.08 microM), also blocks KCNQ2 currents (EC50 = 1.5 microM). Using in situ hybridization, KCNQ2 and KCNQ3 have been localized within the central nervous system, in which they are expressed in the same areas, mainly in the hippocampus, the neocortex and the cerebellar cortex. During brain development, KCNQ3 is expressed later than KCNQ2.