gebhart/patrepeval huggingface dataset:
Three tumor-suppressor regions on chromosome 11p identified by high-resolution deletion mapping in human non-small-cell lung cancer. (1994)

Non-small-cell lung cancer is the leading cause of cancer death for men and women in the industrialized nations. Identification of regions for genes involved in its pathogenesis has been difficult. Data presented here show three distinct regions identified on chromosome 11p. Two regions on 11p13 distal to the Wilms tumor gene WT1 and on 11p15.5 between the markers HBB and D11S860 are described. The third region on the telomere of 11p15.5 has been previously described and is further delineated in this communication. By high-resolution mapping the size of each of these regions was estimated to be 2-3 megabases. The frequency of somatic loss of genetic information in these regions (57%, 71%, and 45%, respectively) was comparable to that seen in heritable tumors such as Wilms tumor (55%) and retinoblastoma (70%) and suggests their involvement in pathogenesis of non-small-cell lung cancer. Gene dosage analyses revealed duplication of the remaining allele in the majority of cases in the 11p13 and the proximal 11p15.5 region but rarely in the distal 11p15.5 region. In tumors with loss of heterozygosity in all three regions any combination of duplication or simple deletion was observed, suggesting that loss of heterozygosity occurs independently and perhaps at different points in time. These results provide a basis for studies directed at cloning potential tumor-suppressor genes in these regions and for assessing their biological and clinical significance in non-small-cell lung cancer.
<title>Panchromatic band sharpening of multispectral image data to improve machine exploitation accuracy</title> (1994)

Due to bandwidth limitations, many remote sensing systems have strengths in either spatial or spectral resolution, an inevitable tradeoff. It is therefore important to have capabilities to merge data and take advantage of the strengths of each. In this paper, we describe a method for enhancing the spatial resolution of multispectral images using a higher resolution panchromatic image. Our method uses the local correlation between low resolution multispectral and corresponding panchromatic radiances to generate sharpened products. The resultant sharpened multispectral images not only have improved visual interpretability but also preserve radiometric fidelity for accurate machine exploitation. This is substantiated by evaluation of the quality of our band sharpening products in terms of machine exploitation accuracy. Results of a sensitivity analysis of the sharpening algorithm to noise are also presented.
Intradialytic creatine supplementation: A scientific rationale for improving the health and quality of life of dialysis patients (2016)

The CK/PCr-system, with creatine (Cr) as an energy precursor, plays a crucial role in cellular physiology. In the kidney, as in other organs and cells with high and fluctuating energy requirements, energy-charged phospho-creatine (PCr) acts as an immediate high-energy source and energy buffer, and as an intracellular energy transport vehicle. A maximally filled total Cr (Cr plus PCr) pool is a prerequisite for optimal functioning of the body and its organs, and health. Skeletal- and cardiac muscles of dialysis patients with chronic kidney disease (CKD) are depleted of Cr in parallel with the duration of dialysis. The accompanying accumulation of cellular damage seen in CKD patients lead to a deterioration of musculo-skeletal and neurological functioning and poor quality of life (QOL). Therefore, to counteract Cr depletion, it is proposed to supplement CKD patients with Cr. The anticipated benefits include previously documented improvements in the musculo-skeletal system, brain and peripheral nervous system, as well as improvements in the common comorbidities of CKD patients (see below). Thus, with a relatively simple, safe and inexpensive Cr supplementation marked improvements in quality of life (QOL) and life span are likely reached. To avoid Cr and fluid overload by oral Cr administration, we propose intradialytic Cr supplementation, whereby a relatively small amount of Cr is added to the large volume of dialysis solution to a final concentration of 1-10mM. From there, Cr enters the patient's circulation by back diffusion during dialysis. Because of the high affinity of the Cr transporter (CRT) for Cr affinity for Cr (Vmax of CRT for Cr=20-40μM Cr), Cr is actively transported from the blood stream into the target cells and organs, including skeletal and cardiac muscle, brain, proximal tubules of kidney epithelial cells, neurons, and leukocytes and erythrocytes, which all express CRT and depend on the CK/PCr system. By this intradialytic strategy, only as much Cr is taken up by the body as is needed to fill the tissue Cr pools and no excess Cr has to be excreted, as is the case with oral Cr. Because aqueous solutions of Cr are not very stable, Cr must be added immediately before dialysis either as solid Cr powder or from a frozen Cr stock solution to the dialysate, or alternatively, Cr could become an additional component of a novel dry dialysate mixture in a cartridge device.
Loss of the common "A" determinant of hepatitis B surface antigen by a vaccine-induced escape mutant. (1992)

A previous study (Carman, W. F., A. R. Zanetti, P. Karayiannis, J. A. Waters, G. Manzillo, E. Tanzi, A. J. Zuckerman, and H. C. Thomas. 1990. Lancet. 336:325-329) demonstrated a variant hepatitis B surface antigen (HBsAg) in a vaccinated child born to a hepatitis B virus-infected mother. A substitution of arginine for glycine at amino acid 145 in HBsAg was observed. In this study the effect of this substitution on the common "a" determinant of this protein, against which protective immunity is directed, is investigated. Using recombinant HBsAg with and without the amino acid substitution, the binding of monoclonal antibodies that recognize different epitopes of the "a" determinant, was shown to be destroyed by the presence of arginine at amino acid 145. In convalescent and vaccinee sera, antibody binding to HBsAg was not inhibited by the variant HBsAg. Immunization with the variant HBsAg, although eliciting a high titer antibody that recognized the variant, produced a low titer of antibody recognizing the native protein. Studies in mice demonstrate that the immunogenicity of the variant protein is also substantially altered. The data presented here demonstrate that this variant evades the known protective anti-HBs response and lends support to the suggestion that this mutation arose as the result of immune pressure.
Abstract A12: Vicoryx: A phase I/IIa clinical trial using a p16INK4a derived peptide as vaccine in patients with advanced human papillomavirus-associated cancer (2013)

Abstract Objectives: The tumor suppressor p16INK4a is strongly overexpressed in HPV-associated neoplasia, whereas in normal tissues barely any p16INK4a expression is detectable. Targeting this HPV type-independent antigen by vaccination could represent an interesting complementary therapeutic approach to recently developed HPV-E6/E7-based therapeutic or secondary preventive vaccines. We performed a phase I/IIa peptide vaccination trial to monitor toxicity and immunogenicity of p16INK4a vaccination in patients with advanced HPV-associated cancers. Patients and Methods: 21 patients with p16INK4a-overexpressing, HPV DNA-positive advanced cancers (16 cervical, 5 head and neck) were included. The protocol comprised 12 applications of a synthetic 27mer p16INK4a peptide mixed with Montanide® ISA-51 VG over a six months period. Objectives of the study were clinical safety and changes of humoral and cellular immune responses against the p16INK4a peptide. T cell responses were monitored by interferon-gamma ELISpot and antibodies by ELISA. Conclusions: No vaccine-related toxicity was observed in any of the patients. One patient (head and neck cancer) completed the entire study protocol with stable disease for now 18 months after the last vaccination.11 patients had progressing disease and were excluded from the study after 4 to 12 weeks. 9 patients continue to be vaccinated. While at baseline only one patient had pre-existing T cell responses (CD4) against the p16INK4a peptide, p16INK4a-reactive T cells (CD4) were successfully induced in at least four patients after 4 to 12 vaccine doses. None of the patients had pre-existing p16INK4a antibodies, but 3 patients developed increasing p16INK4a peptide-specific antibody titers after the 5th dose. This is the first study demonstrating that p16INK4a peptide vaccination is safe and well tolerated. The results show that spontaneous immune responses against p16INK4a are rare, but can be induced by p16INK4a peptide vaccination. Further studies are needed to assess clinical efficacy of the approach. Please refer to the protocol of this trial under http://clinicaltrials.gov/show/NCT01462838. Citation Format: Miriam Reuschenbach, Julia Karbach, Franziska Faulstich, Madeleine Sauer, Matthias Kloor, Mohammad-Reza Rafiyan, Claudia Pauligk, Salah AlBatran, Elke Jaeger, Magnus von Knebel Doeberitz. Vicoryx: A phase I/IIa clinical trial using a p16INK4a derived peptide as vaccine in patients with advanced human papillomavirus-associated cancer. [abstract]. In: Proceedings of the Twelfth Annual AACR International Conference on Frontiers in Cancer Prevention Research; 2013 Oct 27-30; National Harbor, MD. Philadelphia (PA): AACR; Can Prev Res 2013;6(11 Suppl): Abstract nr A12.
Comparison of Stromal/Stem Cells Isolated from Human Omental and Subcutaneous Adipose Depots: Differentiation and Immunophenotypic Characterization (2014)

The emerging field of regenerative medicine has identified adipose tissue as an abundant source of stromal/stem cells for tissue engineering applications. Therefore, we have compared the differentiation and immunophenotypic features of adipose-derived stromal/stem cells (ASC) isolated from either omental or subcutaneous adipose depots. Human tissue samples were obtained from bariatric and plastic surgical practices at a university-affiliated teaching hospital and a private practice, respectively, with informed patient consent. Primary cultures of human ASC were isolated from adipose specimens within 24 h of surgery and culture expanded in vitro. The passaged ASC were induced to undergo adipogenic or osteogenic differentiation as assessed by histochemical methods or evaluated for surface antigen expression profiles by flow cytometry. ASC yields per unit weight of tissue were comparable between omental and subcutaneous depots. At passage 0, the immunophenotype of omental and subcutaneous ASC were not significantly different with the exception of CD105 and endoglin, a component of the transforming growth factor β receptor. The adipogenic differentiation of omental ASC was less robust than that of subcutaneous ASC based on in vitro histochemical and PCR assays. Although the yield and immunophenotype of ASC from omental adipose depots resembled that of subcutaneous ASC, omental ASC displayed significantly reduced adipogenic differentiation capacity following chemical induction. Further studies are necessary to evaluate and optimize the differentiation function of omental ASC in vitro and in vivo. Pending such analyses, omental ASC should not be used interchangeably with subcutaneous ASC for regenerative medical applications.
Template Free and Binderless NiO Nanowire Foam for Li-ion Battery Anodes with Long Cycle Life and Ultrahigh Rate Capability (2016)

Herein, NiO-decorated Ni nanowires with diameters ca. 30-150 nm derived from Ni wire backbone (ca. 2 μm in diameter) is directly synthesized on commercially available Ni foam as a renovated anode for Li-ion batteries. Excellent stability with capacity 680 mAh g(-1) at 0.5C (1C = 718 mA g(-1)) is achieved after 1000 cycles. Superior rate capability is exhibited by cycling at extremely high current rates, such as 20C and 50C with capacities ca. 164 and 75 mAh g(-1), respectively. The capacity can be recovered back to ca. 430 mAh g(-1) in 2 cycles when lowered to 0.2C and stably cycled for 430 times with capacity 460 mAh g(-1). The NiO nanowire foam anode possesses low equivalent series resistance ca. 3.5 Ω, resulting in superior power performance and low resistive losses. The NiO nanowire foam can be manufactured with bio-friendly chemicals and low temperature processes without any templates, binders and conductive additives, which possesses the potential transferring from lab scale to industrial production.
Preconditioned iterative inter-carrier interference cancellation for OFDM reception in rapidly varying channels (2010)

The attractiveness of OFDM decreases with the rising of inter-carrier interference in quickly time-varying channels. Classical OFDM low-complex detection is impaired and more elaborated techniques are required to mitigate the need for full matrix equalization. We present here a fresh approach to this subject, introducing novel fast-converging iterative techniques based on preconditioning. Moreover, we interpret windowing under a new perspective in association with the Basis Expansion Modeling of the time-varying channel. We discuss the complexity of the proposed methods, showing that they are still linear to the OFDM block size. We conclude by illustrating their competitive performance by means of numerical simulations.
AMPEROMETRIC GAS SENSOR USING SOLID STATE PROTON CONDUCTOR SENSITIVE TO HYDROGEN IN AIR AT ROOM TEMPERATURE (1984)

Abstract A new type of amperometric gas sensor using a proton conductor (antimonic acid) and its working mechanisms are proposed for detecting small amounts of H2 in air at room temperature. The short circuit current of the sensor cell was found to be proportional to the concentration of H2. It is also shown that the sensor could be modified into a simpler construction where the reference gas (air) is no longer necessary.
A New Nerve Growth Factor-Mimetic Peptide Active on Neuropathic Pain in Rats (2008)

Analysis of the structure of nerve growth factor (NGF)-tyrosine kinase receptor A (TrkA) complex, site-directed mutagenesis studies and results from chemical modification of amino acid residues have identified loop 1, loop 4, and the N-terminal region of the NGF molecule as the most relevant for its biological activity. We synthesized several peptides mimicking the two loops (1 and 4) linked together with an appropriate spacer, with or without the N-terminal region. Two peptides named NL1L4 and L1L4 demonstrated good NGF agonist activity at a concentration as low as 3 mum. They induced differentiation of chick dorsal root ganglia and stimulated tyrosine phosphorylation of TrkA, but not TrkB, receptor. In addition L1L4 was able to induce differentiation of PC12 cells. More interestingly, the peptide with the highest "in vitro" activity (L1L4) was shown to reduce neuropathic behavior and restore neuronal function in a rat model of peripheral neuropathic pain, thereby suggesting a potential therapeutic role for this NGF-mimetic peptide.
686 PB 230 RESEARCH AND DEVELOPMENT OF A MINIATURE PAN EVAPORIMETER FOR GAUGING THE DIFFERENCE BETWEEN EVAPOTRANSPIRATION AND EFFECTIVE RAINFALL (1994)

Knowledge of the current irrigation requirement of well-watered grass provides the basis for efficient scheduling of turf and landscape irrigation. A portable, miniature pan evaporimeter has been developed to conveniently provide this information for localized micro-climates. The underlying equation for the instrument is: IRnet = (Kpan • Epan - Kpan • R) where IRnet is the net irrigation requirement of healthy, non-stressed grass; Kpan is the pan coefficient for the instrument; Epan is accumulative pan evaporation; Kpan • Epan is “reference evapotranspiration”; and Kpan • R is a measure of effective rainfall received. This equation was established using turfgrass sites located throughout the Pacific Northwest over a 3-year period. The sites were in proximity to U.S. Class “A” pan evaporimeters, and were automatically irrigated using moisture sensors. Tests of the miniature evaporimeter against automated meteorological stations have determined the factors that influence its pan coefficient, and therefore its ultimate design.
High‐performance capillary electrophoresis to determine intact keratan sulfate and hyaluronic acid in animal origin chondroitin sulfate samples and food supplements (2020)

Chondroitin sulfate is extracted from animal cartilaginous tissues and is commercialized as active principle against osteoarthritis. Its biological activity depends on its purity grade and could be altered by the presence of other glycosaminoglycans like keratan sulfate that could be contemporarily extracted from animal tissues or like hyaluronic acid that, instead, is added on purpose in food supplements. Although numerous methods are reported in literature for quality control analyses of chondroitin sulfate, few of them are able to detect other glycosaminoglycans. In this paper, for the first time, a new high-performance CE method was set up to quantify the chondroitin sulfate, the eventual keratan sulfate, and hyaluronic acid as intact chains: five chondroitin sulfate standards and 13 animal origin samples or food supplements from six different suppliers were analyzed. The new method was able to determine keratan sulfate similarly to a previously reported high-performance anion-exchange chromatography method, but in addition it showed the advantage to determine also the hyaluronic acid as never reported before.
A SEX ATTRACTANT FOR ADULT MALES OF THE PALE WESTERN CUTWORM, <i>AGROTIS ORTHOGONIA</i> (LEPIDOPTERA: NOCTUIDAE) (1978)

Abstract Field testing of a large number of synthetic compounds and many combinations of them revealed that male moths of the pale western cutworm, Agrotis orthogonia Morrison, were attracted to combinations of Z -5-dodecen-1-yl acetate and Z -7-dodecen-1-yl acetate. The most satisfactory attractants were combinations of these two compounds in ratios of 1:2 to 1:5 at 10 to 25 μg/septum dispenser. The effect of the attractant was strongly inhibited by the addition of 21% Z -7-dodecen-1-ol.
Biocompatible artificial DNA linker that is read through by DNA polymerases and is functional in <i>Escherichia coli</i> (2011)

A triazole mimic of a DNA phosphodiester linkage has been produced by templated chemical ligation of oligonucleotides functionalized with 5'-azide and 3'-alkyne. The individual azide and alkyne oligonucleotides were synthesized by standard phosphoramidite methods and assembled using a straightforward ligation procedure. This highly efficient chemical equivalent of enzymatic DNA ligation has been used to assemble a 300-mer from three 100-mer oligonucleotides, demonstrating the total chemical synthesis of very long oligonucleotides. The base sequences of the DNA strands containing this artificial linkage were copied during PCR with high fidelity and a gene containing the triazole linker was functional in Escherichia coli.
Isomeric phenylthioimidazo[1,2-a]pyridines as anthelmintics (1981)

A series of isomeric imidazo[1,2-alpha]pyridine-2-carbamates was prepared for testing as anthelmintics. The analogues were synthesized by reacting the appropriate 2-aminopyridine and methyl chloroacetylcarbamate. Steric hindrance in the 2,6-disubstituted derivative resulted in the formation of the isomeric 3-substituted analogue as the major product. Carbon-13 NMR proved useful in the structural assignments in this series. None of the analogues exhibited the potency of methyl 6-(phenylsulfinyl)imidazo[1,2-alpha]pyridine-2-carbamate when tested against Nematospiroides dubius in mice.
Loss in hollow-core optical fibers: mechanisms, scaling rules, and limits (2022)

Over the past few years, progress in hollow-core optical fiber technology has reduced the attenuation of these fibers to levels comparable to those of all-solid silica-core single-mode fibers. The sustained pace of progress in the field has sparked renewed interest in the technology and created the expectation that it will one day enable realization of the most transparent light-propagating waveguides ever produced, across all spectral regions of interest. In this work we review and analyze the various physical mechanisms that drive attenuation in hollow-core optical fibers. We consider both the somewhat legacy hollow-core photonic bandgap technology as well as the more recent antiresonant hollow-core fibers. As both fiber types exploit different guidance mechanisms from that of conventional solid-core fibers to confine light to the central core, their attenuation is also dominated by a different set of physical processes, which we analyze here in detail. First, we discuss intrinsic loss mechanisms in perfect and idealized fibers. These include leakage loss, absorption, and scattering within the gas filling the core or from the glass microstructure surrounding it, and roughness scattering from the air–glass interfaces within the fibers. The latter contribution is analyzed rigorously, clarifying inaccuracies in the literature that often led to the use of inadequate scaling rules. We then explore the extrinsic contributions to loss and discuss the effect of random microbends as well as that of other perturbations and non-uniformities that may result from imperfections in the fabrication process. These effects impact the loss of the fiber predominantly by scattering light from the fundamental mode into lossier higher-order modes and cladding modes. Although these contributions have often been neglected, their role becomes increasingly important in the context of producing, one day, hollow-core fibers with sub-0.1-dB/km loss and a pure single-mode guidance. Finally, we present general scaling rules for all the loss mechanisms mentioned previously and combine them to examine the performance of recently reported fibers. We lay some general guidelines for the design of low-loss hollow-core fibers operating at different spectral regions and conclude the paper with a brief outlook on the future of this potentially transformative technology.
Fourier transform ion cyclotron resonance mass spectrometry at the true cyclotron frequency (2021)

Ion cyclotron resonance (ICR) cells provide stability and coherence of ion oscillations in crossed electric and magnetic fields over extended periods of time. Using the Fourier transform enables precise measurements of ion oscillation frequencies. These precisely measured frequencies are converted into highly accurate mass-to-charge ratios of the analyte ions by calibration procedures. In terms of resolution and mass accuracy, Fourier transform ICR mass spectrometry (FT-ICR MS) offers the highest performance of any MS technology. This is reflected in its wide range of applications. However, in the most challenging MS application, for example, imaging, enhancements in the mass accuracy of fluctuating ion fluxes are required to continue advancing the field. One approach is to shift the ion signal power into the peak corresponding to the true cyclotron frequency instead of the reduced cyclotron frequency peak. The benefits of measuring the true cyclotron frequency include increased tolerance to electric fields within the ICR cell, which enhances frequency measurement precision. As a result, many attempts to implement this mode of FT-ICR MS operation have occurred. Examples of true cyclotron frequency measurements include detection of magnetron inter-harmonics of the reduced cyclotron frequency (i.e., the sidebands), trapping field-free (i.e., screened) ICR cells, and hyperbolic ICR cells with quadrupolar ion detection. More recently, ICR cells with spatially distributed ion clouds have demonstrated attractive performance characteristics for true cyclotron frequency ion detection. Here, we review the corresponding developments in FT-ICR MS over the past 40 years.