gebhart/patrepeval huggingface dataset:
Multichannel power electronics and magnetic nanoparticles for selective thermal magnetogenetics (2022)

<i>Objective.</i>We present a combination of a power electronics system and magnetic nanoparticles that enable frequency-multiplexed magnetothermal-neurostimulation with rapid channel switching between three independent channels spanning a wide frequency range.<i>Approach.</i>The electronics system generates alternating magnetic field spanning 50 kHz to 5 MHz in the same coil by combining silicon (Si) and gallium-nitride (GaN) transistors to resolve the high spread of coil impedance and current required throughout the wide bandwidth. The system drives a liquid-cooled field coil via capacitor banks, forming three series resonance channels which are multiplexed using high-voltage contactors. We characterized the system by the output channels' frequencies, field strength, and switching time, as well as the system's overall operation stability. Using different frequency-amplitude combinations of the magnetic field to target specific magnetic nanoparticles with different coercivity, we demonstrate actuation of iron oxide nanoparticles in all three channels, including a novel nanoparticle composition responding to magnetic fields in the megahertz range.<i>Main results.</i>The system achieved the desired target field strengths for three frequency channels, with switching speed between channels on the order of milliseconds. Specific absorption rate measurements and infrared thermal imaging performed with three types of magnetic nanoparticles demonstrated selective heating and validated the system's intended use.<i>Significance.</i>The system uses a hybrid of Si and GaN transistors in bridge configuration instead of conventional amplifier circuit concepts to drive the magnetic field coil and contactors for fast switching between different capacitor banks. Series-resonance circuits ensure a high output quality while keeping the system efficient. This approach could significantly improve the speed and flexibility of frequency-multiplexed nanoparticle actuation, such as magnetogenetic neurostimulation, and thus provide the technical means for selective stimulation below the magnetic field's fundamental spatial focality limits.
[Free radicals and respiratory pathology]. (1994)

The lung is particularly exposed to various inhaled toxic products whose toxicity can be at least partly mediated by the generation of free radicals. Oxidants burden can also result from lung metabolism of xenobiotics or from activation of phagocytes. Free radicals are mainly derived from an univalent sequential reduction of molecular oxygen. Mitochondria is the main location of intracellular production which may also result from auto-oxidation of small molecules or function of some enzymes. To prevent the deleterious effects of free radicals produced by normal metabolism, cells are equipped with an antioxidant system composed of enzymes (superoxide dismutase, catalase, glutathione peroxidase) and non enzymatic substances such as glutathione, iron chelators, vitamin E and C, ceruleoplsamin). Targets of free radicals toxicity are phospholipids by initiation of lipid peroxidation, proteins which may be activated or inactivated via oxidation of sulfhydryl residues. Another target is DNA with possible strand breaks or mutation. Transcription activities can be also altered and it has been recently reported that some transcription factors such as NF-kB can be activated by oxidants. Under these circumstances free radicals may be considered as second messengers. Lung oxygen toxicity has been largely studied. Oxygen-induced lung lesions are non specific. It is possible to induce a resistance to 100% O2 by the pre-exposure of animals to 85% O2. This tolerance phenomenon is associated with an increased lung content in antioxidant substances. The mechanisms of gene regulation of antioxidant enzymes are still poorly understood in eukaryotes. Overproduction of free radicals in the lung is also involved in various clinical settings such as ischemia-reperfusion, exposure to ozone or NO2, acute respiratory distress syndrome, drug induced lung toxicity, pathogenesis of COPD, asthma, cancer and ageing. The precise role of free radicals among other mechanisms of lung injury is still unclear. A better knowledge of free radicals mechanisms of toxicity and of antioxidant regulation is needed to develop antioxidant therapeutic strategies.
Mutational Analysis of Centromere DNA from Chromosome VI of <i>Saccharomyces cerevisiae</i> (1988)

Saccharomyces cerevisiae centromeres have a characteristic 120-base-pair region consisting of three distinct centromere DNA sequence elements (CDEI, CDEII, and CDEIII). We have generated a series of 26 CEN mutations in vitro (including 22 point mutations, 3 insertions, and 1 deletion) and tested their effects on mitotic chromosome segregation by using a new vector system. The yeast transformation vector pYCF5 was constructed to introduce wild-type and mutant CEN DNAs onto large, linear chromosome fragments which are mitotically stable and nonessential. Six point mutations in CDEI show increased rates of chromosome loss events per cell division of 2- to 10-fold. Twenty mutations in CDEIII exhibit chromosome loss rates that vary from wild type (10(-4)) to nonfunctional (greater than 10(-1)). These results directly identify nucleotides within CDEI and CDEIII that are required for the specification of a functional centromere and show that the degree of conservation of an individual base does not necessarily reflect its importance in mitotic CEN function.
Axial polarization switching in ferroelectric BaTiO<sub>3</sub>nanowire (2007)

We report the study of direct ferroelectric polarization switching of a single crystal BaTiO3 nanowire along its length axis. Polarization switching under axial dc bias, monitored with lateral mode piezoresponse force microscopy, was shown to be non-remnant. The result suggested the existence of a preferred polarization orientation in BaTiO3 nanowire, i.e., a polarization imprint, and was explained with a core–shell polarization structure model. The polarization shell thickness was estimated to be ∼10 nm for a BaTiO3 nanowire having a thickness of ∼120 nm.
Places Clustering of Full-Length Film Key-Frames Using Latent Aspect Modeling Over SIFT Matches (2009)

An improved unsupervised classification method to extract and link places features and cluster recurrent physical locations (key-places) within a movie is presented. Our approach finds links between key frames of a common key-place based on the use of a probabilistic latent space model over the possible local matches between the key frames image set. This allows the extraction of significant groups of local matching descriptors that may represent characteristic elements of a key-place. An exhaustive evaluation of our approach was conducted on in-house and public image datasets, as well as on full-length movies. Results revealed that our method is very efficient for near-duplicate object/background detection with weak overlap. Performance measurements on full-length movies indicate a recognition rate of about 75% on the key-places clustering with a false alarm rate (FAR) of approximately 2%.
Processes of nonconsumable electrode welding with welding current modulation (review) part iii. modeling of the processes of TIG welding by modulated current (2020)

Automatic Welding, 2020, №01. Journal «AVTOMATICHESKAYA SVARKA» Monthly scientific-technical and production journal. ISSN: 0005-111X, Since 1948. Russian version of «The PATON Welding Journal» Text in Russian, contents and summaries in English. The Journal presents a wide range of scientific-technical and promotion information in the field of technologies, equipment and materials for welding, surfacing, brazing, and deposition of protective coatings.
[{Cp<sup>*</sup>Cr(μ<sub>3</sub>‐H)}<sub>4</sub>]—a Paramagnetic Chromium Hydride with a Cubane Structure (1992)

For the exploration of the organometallic chemistry of divalent chromium, the readily synthesized CrII complex 1 is a simple starting material. The chloro bridges can be exchanged for alkyl groups (R = Me, nBu, CH2SiMe3), and the resulting complexes 2 react with H2 to give the tetranuclear hydrido complex 3 with a cubane structure. Unexpectedly, 3 shows almost ideal Curie paramagnetism according to magnetic measurements.
Theoretical Analysis of Osmotic Agents in Peritoneal Dialysis. What Size is An Ideal Osmotic Agent? (1996)

In this article the difference between osmotic fluid flow (ultrafiltration) as driven by osmotic pressure and diffusion through thin leaky membranes is discussed. It is pointed out that water transport induced by osmosis is fundamentally different from the process of water diffusion. Applying modern hydrodynamic pore theory, the molar solute concentration and the solute concentration in grams per 100 mL, exerting the same initial transmembrane osmotic pressure as a 1% glucose solution, was investigated as a function of solute molecular weight (MW). It was then assumed, base on experimental data, that the major pathway responsible for the peritoneal osmotic barrier characteristics is represented by pores of radius approximately 47 A. With increasing solute radius, the osmotic reflection coefficient (sigma) and, hence, the osmotic efficiency per mole of solute will increase. However, simultaneously, the molar concentration per unit solute weight will decrease. The balance point between these two events apparently occurs at a solute MW of approximately 1 kDa. An additional advantage of using solutes of high MW as osmotic agents during peritoneal dialysis (PD), rather than increased osmotic efficiency per se, lies in the fact that large solutes, due to their low peritoneal diffusion capacity, will maintain a sustained rate of ultrafiltration (osmosis) over a prolonged period. To illustrate this, we have performed computer simulations of peritoneal fluid transport according to the three-pore model of peritoneal permselectivity. According to these simulations, 4% of an 800 Da polymer solution (+50 mmol/L above isotonicity) will produce the same cumulative amount of intraperitoneal fluid volume ultrafiltered (UF) during 360-400 minutes as 4% of a 2 kDa polymer solution (+20 mmol/L) or 6.5% of a 10 kDa polymer solution (+6.5 mmol/L) having the same electrolyte concentration as dialysis solutions conventionally used for PD. Similar cumulative UF volumes (during 400 minutes) can be obtained by a 2.5% glycerol (+272 mmol/L) or a 3.2% glucose-containing dialysis solution (+177 mmol/L) with conventional electrolyte composition.
[Analysis of APC gene mutation in a familial adenomatous polyposis pedigree]. (2014)

The c.2891T>G (L964X) of the APC gene probably underlies the familial adenomatous polyposis in this pedigree. The combined DNA sequencing and PCR-RFLP method is efficient and accurate for the diagnosis.
HEIGHT ESTIMATION FROM A SINGLE CAMERA VIEW (2012)

This paper presents a novel technique for the estimation of the height of an object using a single camera view. In the proposed method, the only information required is the knowledge about the pose of the camera with respect to the world (i.e., height and pitch angle of the camera with respect to the ground) and a vanishing point. In the developed theory, the focal length may also be known, but in the proposed experiments it has not been employed: an approximation for small pitch angles has been taken into account and the consequent committed error has been then analysed. The presented method gives accurate results for any object placed in unstructured environments, regardless of the relative distance from the camera. The method has been tested in a series of outdoor and indoor environments, and the experimental results are presented in this paper.
Scuid^Sim: A Platform for Smart Card User Interface Research, Development and Testing (2014)

The latest advances in the field of smart card technologies allow modern cards to be more than just simple security tokens. Recent developments facilitate the use of interactive components like buttons, displays or even touch-sensors within the cards body thus conquering whole new areas of application. With interactive functionalities the usability aspect becomes the most important one for designing secure and popularly accepted products. Unfortunately the usability can only be tested fully with completely integrated hence expensive smart card prototypes. This restricts application specific research, case studies of new smart card user interfaces, concerning applications and the performance of useability tests in smart card development. Rapid development and simulation of smart card interfaces and applications can help to avoid this restriction. This paper presents SCUIDtextsuperscript{Sim} a tool for rapid user-centric development of new smart card interfaces and applications based on common smartphone technology.
Role of Enteroviral RNA-Dependent RNA Polymerase in Regulation of MDA5-Mediated Beta Interferon Activation (2019)

Infection by enteroviruses can cause severe neurological complications in humans. The interactions between the enteroviral and host proteins may facilitate the virus replication and be involved in the pathogenicity of infected individuals. It has been shown that human enteroviruses possess various mechanisms to suppress host innate immune responses in infected cells. Previous studies showed that infection by enterovirus 71 (EV71) causes the degradation of MDA5, which is a critical cytoplasmic pathogen sensor in the recognition of picornaviruses for initiating transcription of type I interferons. In the present study, we demonstrated that the RNA-dependent RNA polymerase (RdRP; also denoted 3D<sup>pol</sup>) encoded by EV71 interacts with the caspase activation and recruitment domains (CARDs) of MDA5 and plays a role in the inhibition of MDA5-mediated beta interferon (IFN-β) promoter activation and mRNA expression. In addition, we found that the 3D<sup>pol</sup> protein encoded by coxsackievirus B3 also interacted with MDA5 and downregulated the antiviral signaling initiated by MDA5. These findings indicate that enteroviral RdRP may function as an antagonist against the host antiviral innate immune response.<b>IMPORTANCE</b> Infection by enteroviruses causes severe neurological complications in humans. Human enteroviruses possess various mechanisms to suppress the host type I interferon (IFN) response in infected cells to establish viral replication. In the present study, we found that the enteroviral 3D<sup>pol</sup> protein (or RdRP), which is a viral RNA-dependent RNA polymerase for replicating viral RNA, plays a role in the inhibition of MDA5-mediated beta interferon (IFN-β) promoter activation. We further demonstrated that enteroviral 3D<sup>pol</sup> protein interacts with the caspase activation and recruitment domains (CARDs) of MDA5. These findings indicate that enteroviral RdRP functions as an antagonist against the host antiviral response.
Identification of the 37-kDa Antigen in IDDM as a Tyrosine Phosphatase-Like Protein (Phogrin) Related to IA-2 (1996)

Antibodies to islet cell proteins detected as 37,000 and 40,000 M(r), tryptic fragments (37- and 40-kDa antigens) are strongly associated with progression to IDDM. The 40-kDa antigen has recently been identified as the tyrosine phosphatase-like protein IA-2 (ICA512) whereas the 37-kDa antigen has been suggested to be a different protein that has structural similarity to IA-2. A protein, phogrin, that has 80% amino acid sequence identity to IA-2 in the cytoplasmic domain, has recently been cloned from an insulinoma cell cDNA library. In this study, we have investigated possible relationships between the 37-kDa antigen and phogrin. Antibodies to phogrin were detected in sera from patients with IDDM, and these antibodies were strongly correlated with the presence of antibodies to the 37-kDa antigen. Trypsin treatment of immunoprecipitated phogrin generated a 37,000 M(r) fragment. Recombinant phogrin was able to block autoantibody binding to the 37-kDa antigen but not to the 40-kDa antigen, and rabbit antibodies raised to different regions of phogrin depleted insulinoma cell extracts specifically of the 37-kDa antigen. These results demonstrate that the 37-kDa antigen in IDDM is indistinguishable from phogrin and show that two distinct tyrosine phosphatase-related proteins are major targets of the autoimmune response in the disease.
Interactions between lymphocytes and myeloid cells regulate pro- versus anti-tumor immunity (2010)

Tumor-associated myeloid cells have been implicated in regulating many of the "hallmarks of cancer" and thus fostering solid tumor development and metastasis. However, the same innate leukocytes also participate in anti-tumor immunity and restraint of malignant disease. While many factors regulate the propensity of myeloid cells to promote or repress cancerous growths, polarized adaptive immune responses by B and T lymphocytes have been identified as regulators of many aspects of myeloid cell biology by specifically regulating their functional capabilities. Here, we detail the diversity of heterogeneous B and T lymphocyte populations and their impacts on solid tumor development through their abilities to regulate myeloid cell function in solid tumors.
The ECO data set and the performance of non-intrusive load monitoring algorithms (2014)

Non-intrusive load monitoring (NILM) is a popular approach to estimate appliance-level electricity consumption from aggregate consumption data of households. Assessing the suitability of NILM algorithms to be used in real scenarios is however still cumbersome, mainly because there exists no standardized evaluation procedure for NILM algorithms and the availability of comprehensive electricity consumption data sets on which to run such a procedure is still limited. This paper contributes to the solution of this problem by: (1) outlining the key dimensions of the design space of NILM algorithms; (2) presenting a novel, comprehensive data set to evaluate the performance of NILM algorithms; (3) describing the design and implementation of a framework that significantly eases the evaluation of NILM algorithms using different data sets and parameter configurations; (4) demonstrating the use of the presented framework and data set through an extensive performance evaluation of four selected NILM algorithms. Both the presented data set and the evaluation framework are made publicly available.
Polyglutamine aggregation nucleation: Thermodynamics of a highly unfavorable protein folding reaction (2005)

Polyglutamine (polyGln) aggregation is implicated in the disease progression of Huntington's disease and other expanded CAG repeat diseases. PolyGln aggregation in vitro follows a simple nucleated growth polymerization pathway without apparent complications such as populated intermediates, alternative assembly pathways, or secondary nucleation phenomena. Previous analysis of the aggregation of simple polyGln peptides revealed that the critical nucleus (the number of monomeric units involved in the formation of an energetically unfavorable aggregation nucleus) is equal to one, suggesting that polyGln nucleation can be viewed as an unfavorable protein folding reaction. We provide here a method for experimentally determining the number of elongation growth sites per unit weight for any polyGln aggregate preparation, a key parameter required for completion of the nucleation kinetics analysis and determination of the thermodynamics of nucleation. We find that, for the polyGln peptide Q(47), the second-order rate constant for fibril elongation is 11,400 liters/mol per s, whereas K(n*)), the equilibrium constant for nucleation of aggregation, is remarkably small, equal to 2.6 x 10(-9). The latter value corresponds to a free energy of nucleus formation of +12.2 kcal/mol, a value consistent with a highly unfavorable folding reaction. The methods introduced here should allow further analysis of the energetics of polyGln nucleus formation and accurate comparisons of the seeding capabilities of different fibril preparations, a task of increasing importance in the amyloid field.
Integrated frameworks for multi-level and multi-formalism modeling (2003)

There have been significant advances in methods for specifying and solving models that aim to predict the performance and dependability of computer systems and networks. At the same time, however, there have been dramatic increases in the complexity of the systems whose performance and dependability must be evaluated, and considerable increases in the expectations of analysts that use performance/dependability evaluation tools. This paper briefly reviews the progress that has been made in the development of performance/dependability evaluation tools, and argues that the next important step is the creation of modeling frameworks and software environments that support multi-level, multi-formalism modeling and multiple solution methods within a single integrated framework. In addition, this paper presents an overview of the Mobius project, which aims to provide a modeling framework and software environment that support multiple modeling formalisms, methods for model composition and connection, and a way to integrate multiple analytical/numerical- and simulation-based model solution methods. Finally, it suggests research that must take place to make this aim a reality, and thus facilitate the performance and dependability evaluation of complex computer systems and networks.
Solvophobically Driven Folding of Nonbiological Oligomers (1997)

In solution, biopolymers commonly fold into well-defined three-dimensional structures, but only recently has analogous behavior been explored in synthetic chain molecules. An aromatic hydrocarbon backbone is described that spontaneously acquires a stable helical conformation having a large cavity. The chain does not form intramolecular hydrogen bonds, and solvophobic interactions drive the folding transition, which is sensitive to chain length, solvent quality, and temperature.
Bioprosthetic replacement of the ascending thoracic aorta: what are the options? (2008)

Increasing patient age and improved durability of latest generation bioprostheses have stimulated the use of bioprosthetic devices in the setting of ascending aortic replacement as an alternative to mechanical valved conduits or aortic valve-sparing procedures. We performed an English literature review to assess different surgical options that have been described for bioprosthetic replacement of the ascending aorta. Reported options include: (1) composite valved conduits using a stented bioprosthesis; (2) composite valved conduits using a stentless bioprosthesis; (3) total xenopericardial valved conduits. Composite valved grafts using stented bioprostheses offer a safe and durable option for bioprosthetic replacement of the ascending aorta. Other options are of more recent use and await medium-term results.
Hepatitis non-A, non-B. (1980)

Evidence for the existence of hepatitis non-A, non-B includes epidemiologic data and results of transmission studies indicating the presence of hepatitis that could not be explained by known causative agents. The diagnosis is suggested in patients who have multiple episodes of acute hepatitis or who contract hepatitis after transfusion, hemodialysis or drug abuse. Sporadic cases are common. Three such cases are described to illustrate that the disease is clinically indistinguishable from hepatitis A or B. The diagnosis is based on the absence of serologic markers of hepatitis A and B and of infection by Epstein-Barr virus and cytomegalovirus, or on serologic evidence of previous infection with hepatitis A and B.