gebhart/patrepeval huggingface dataset:
Selection of Peptide Ligands Specific for Baculovirus DNA‐Binding Protein from the Flitrx™ Random Peptide Display Library (2006)

Abstract Autographa californica nucleopolyhedrovirus (AcNPV) is a baculovirus that is widely employed as a vector for the expression of foreign genes and pest control. Although baculoviruses, including AcNPV, efficiently replicate in the nuclei of arthropod cells, the dynamics and mechanism of DNA replication within the infected cell are still poorly understood. It has been found that the DNA‐binding protein (DBP) is an early gene product and appears to be crucial for viral DNA replication. Presented here is the selection of peptide ligands that specifically bind to DBP for AcNPV from the FliTrx™ random peptide display library; this entails the amplification, cloning of the DNA‐binding protein (DBP) gene from AcNPV and the construction of the expression plasmid for DBP, and the expression and purification of the recombinant His.Tag AcNPV DBP that was used as a target molecule for the selection of the peptide ligands specific for AcNPV DBP. The affinity and efficiency of such peptide ligands were then measured by ELISA procedures. The beneficial aspect of this research is the monospecificity quality of the peptide ligands specific for AcNPV DBP. They could be used for the study of the dynamics of the viral genome and its replication within the infected cell, for the development of a quantitative method for the determination of the presence of baculovirus in various samples, for the development of a peptide ligandbased assay for the determination of baculovirus titers; or they could be immobilized on a chromatographic support for an improved affinity purification of AcNPV DBP.
Characterization of a femtosecond kHz amplifier chain by spectral shearing interferometry (1998)

Summary form only given. In a chirped-pulse amplification laser chain, as the spectral phase added by the stretcher and the group-velocity dispersion in all the materials is imperfectly compensated by the compressor, the output pulse is distorted by the residual phase, and its duration and contrast are not optimal. There is thus a need for an efficient characterization of the pulses in order to optimize the system or control a programmable spectral phase modulator. Spectral shearing interferometry is demonstrated as a powerful technique for measuring the amplitude and phase of ultrashort optical pulses. Its implementation is simple, and the phase retrieval from the data is straightforward.
Characterization of Human Palladin, a Microfilament-associated Protein (2001)

Actin-containing microfilaments control cell shape, adhesion, and contraction. In striated muscle, alpha-actinin and other Z-disk proteins coordinate the organization and functions of actin filaments. In smooth muscle and nonmuscle cells, periodic structures termed dense bodies and dense regions, respectively, are thought to serve functions analogous to Z-discs. We describe here identification and characterization of human palladin, a protein expressed mainly in smooth muscle and nonmuscle and distributed along microfilaments in a periodic manner consistent with dense regions/bodies. Palladin contains three Ig-domains most homologous to the sarcomeric Z-disk protein myotilin. The N terminus includes an FPPPP motif recognized by the Ena-Vasp homology domain 1 domain in Ena/vasodilatator-stimulated phosphoprotein (VASP)/Wiscott-Aldrich syndrome protein (WASP) protein family. Cytoskeletal proteins with FPPPP motif target Ena/VASP/WASP proteins to sites of actin modulation. We identified palladin in a yeast two-hybrid search as an ezrin-associated protein. An interaction between palladin and ezrin was further verified by affinity precipitation and blot overlay assays. The interaction was mediated by the alpha-helical domain of ezrin and by Ig-domains 2-3 of palladin. Ezrin is typically a component of the cortical cytoskeleton, but in smooth muscle cells it is localized along microfilaments. These cells express palladin abundantly and thus palladin may be involved in the microfilament localization of ezrin. Palladin expression was up-regulated in differentiating dendritic cells (DCs), coinciding with major cytoskeletal and morphological alterations. In immature DCs, palladin localized in actin-containing podosomes and in mature DCs along actin filaments. The regulated expression and localization suggest a role for palladin in the assembly of DC cytoskeleton.
Liposome-Encapsulated Hemoglobin: A Red Blood Cell Substitute (1993)

A safe and effective red blood cell (RBC) substitute would have broad implications in the practice of emergency medicine, trauma management, surgery, and several other areas of medicine. Several hemoglobin-based RBC substitutes have been developed that can deliver oxygen to peripheral tissues. However, although these RBC substitutes have desirable biophysical properties, their in vivo efficacy is limited by their significant toxicity. In view of the very high doses of blood substitute that are likely to be used clinically, toxicity as well as other safety issues that include hemostasis and thrombosis are critical considerations for the development and ultimate application of RBC surrogates.Recent work conducted in our laboratories has demonstrated that administration of liposome-encapsulated hemoglobin (LEH) in rats was efficacious. Also our results have demonstrated that the replacement of more conventional lipids with the sterically-stabilizing lipid polyethyleneglycol distearoylphosphatidyl-ethanolamine in the LEH results in a significant decrease in LEH immunotoxicity, as measured by host resistance to infectious insult.
Laser-raman monitoring of ambient sulfate aerosols (1977)

The assessment of the mobility and bioavailability of heavy metals in sediment samples requires the use of sequential extraction procedures. Results for sediments taken from an unmanaged motorway detention pond showed heavy metals to be associated with the carbonate and residual fractions. In particular, nickel and cadmium showed a high percentage association, compared with other geochemical fractions, such as carbonates and residual fractions. Copper and zinc were shown to be associated with carbonates and iron-manganese oxides, whereas lead showed the strongest association (72%) with the Fe-Mn oxides. When compared to a groundwater-fed lake on the same site, all heavy metals contained within this sediment were found to be at much lower concentrations and strongly associated with the residual fraction (>50%) and are therefore strongly bound within the sediment. Following discharge via a wet detention pond, stormwater then flows through a series of interconnecting lake systems. Heavy metal fractionation of these interconnecting lakes is discussed and shows changes in fractionation through the lake system for copper, nickel, lead and zinc. The main concern in these lakes is the continued use of calcium carbonate to maintain freshwater pH for coarse fishing. This practice, used until 1999, may present an enhanced risk to the freshwater environment, causing greater mobility of these metals.
PRODIAG: Combined expert system/neural network for process fault diagnosis. Volume 1, Theory (1995)

The function of the PRODIAG code is to diagnose on-line the root cause of a thermal-hydraulic (T-H) system transient with trace back to the identification of the malfunctioning component using the T-H instrumentation signals exclusively. The code methodology is based on the Al techniques of automated reasoning/expert systems (ES) and artificial neural networks (ANN). The research and development objective is to develop a generic code methodology which would be plant- and T-H-system-independent. For the ES part the only plant or T-H system specific code requirements would be implemented through input only and at that only through a Piping and Instrumentation Diagram (PID) database. For the ANN part the only plant or T-H system specific code requirements would be through the ANN training data for normal component characteristics and the same PID database information. PRODIAG would, therefore, be generic and portable from T-H system to T-H system and from plant to plant without requiring any code-related modifications except for the PID database and the ANN training with the normal component characteristics. This would give PRODIAG the generic feature which numerical simulation plant codes such as TRAC or RELAP5 have. As the code is applied to different plants and different T-H systems, only the connectivity information, the operating conditions and the normal component characteristics are changed, and the changes are made entirely through input. Verification and validation of PRODIAG would, be T-H system independent and would be performed only ``once``.
Photonic digital-to-analog conversion and digitally driven integrated optics modulator (2011)

A novel integrated optical modulator based on a multi-electrode Mach-Zehnder modulator has been developed and analyzed. The device has superior performance in terms of linearity, dynamic range and implementation complexity. The improvements are achieved by utilizing a unique mapping method between the analog input and the digital sequence applied to the device, and by an optimized sectioning method for the electrodes. Further improvement in linearity is attained by allowing the number of electrodes to be larger than the number of digital input bits. The device can serve as a photonic digital-to-analog converter, accepting electrical digital signal and converting it into an optical (or electrical) analog signal. The device can also serve as an M-ary modulator for generating optical multi-level signals for coherent optical communication.
A Multivalent Approach to the Design and Discovery of Orally Efficacious 5-HT<sub>4</sub>Receptor Agonists (2009)

5-HT(4) receptor agonists such as tegaserod have demonstrated efficacy in the treatment of constipation predominant irritable bowel syndrome (IBS-C), a highly prevalent disorder characterized by chronic constipation and impairment of intestinal propulsion, abdominal bloating, and pain. The 5-HT(4) receptor binding site can accommodate functionally and sterically diverse groups attached to the amine nitrogen atom of common ligands, occupying what may be termed a "secondary" binding site. Using a multivalent approach to lead discovery, we have investigated how varying the position and nature of the secondary binding group can be used as a strategy to achieve the desired 5-HT(4) agonist pharmacological profile. During this study, we discovered the ability of amine-based secondary binding groups to impart exceptional gains in the binding affinity, selectivity, and functional potency of 5-HT(4) agonists. Optimization of the leads generated by this approach afforded compound 26, a selective, orally efficacious 5-HT(4) agonist for the potential treatment of gastrointestinal motility-related disorders.
Electronic see-through head mounted display with minimal peripheral obscuration (2020)

SA Photonics and SL Process have developed a new type of electronic see-through augmented reality head mounted display. Our display utilizes a very compact freeform prism eyepiece that has almost no peripheral obscuration. This provides the wearer with the advantages of an electronic see-through system (occlusion, operation in high ambient environments, high contrast imagery, image enhancement, image export) but in a system that also allows natural vision of the real world outside the field of view of the eyepiece. As such, the user sees a seamless view of the entire real world, with augmentation in the central 62-degree diagonal field of view. The cameras for the see-through imagery are “hidden” behind the eyepieces, so they do not block the view of the real world, providing less than a 10% obscuration of the total visual field, much less than any other electronic see-through system and even less than many optical see-through systems. In addition to providing the see-through imagery, these cameras and SL Process’ innovative video processing architecture provide six degrees of freedom head tracking as well as hand tracking.
Curve-Based Shape Matching: Supporting Designers' Hierarchies through Parametric Shape Recognition of Arbitrary Geometry (2006)

In this paper we introduce a subshape matching technique for shapes composed of curved lines that lie in a plane. An interpreter with this ability allows for the practical implementation of general shape grammars as well as opening the door for new applications of shape grammars. This method for matching shapes consisting of curved lines is outlined and examples are shown. The emergent properties of shape grammars are also explored and considered using the new method for shape interpretation.
In Vivo Detection of Clonally Derived Methicillin-Resistant/Methicillin-Susceptible <i>Staphylococcus aureus</i> Strains Is Not a Rare Event (2008)

Daskalaki and colleagues ([3][1]) recently reported the identification of mixed clonally derived Staphylococcus aureus isolates that were responsible for recurrent bacteremia and showed important genetic rearrangements during the time frame of an infection. At our institution, in addition to
Reconfigurable Doherty Power Amplifier for Multifrequency Wireless Radio Systems (2013)

This paper proposes a frequency-agile Doherty amplifier capable of efficiently amplifying multiband wireless signals located at widely spaced frequencies. Starting with the study of the load modulation concept in the Doherty technique and the various frequency-dependent components involved in its realization, this paper suggests a simplified and effective design methodology for the development of frequency-agile and reconfigurable Doherty amplifiers using a small number of electronically tunable devices. As a proof of concept, a reconfigurable Doherty power amplifier (PA) prototype is designed and fabricated to operate at 1.9, 2.14, and 2.6 GHz. The measurement results obtained using a continuous-wave signal revealed drain efficiencies of approximately 70% and 60% at the peak power and 6 dB back-off power, respectively, for the three targeted operating frequencies. In addition, the reconfigurable Doherty amplifier was successfully linearized when driven with 20-MHz wavelength code-division multiple access and longterm evolution signals, using a Volterra-based digital predistrtion algorithm which exploits a pruned Volterra series.
Biochemical processing of geothermal brines and sludges (1995)

RNs in ambulatory practice must expand their role to assist primary care physicians meet the growing demands of the aging American population. This article explores the benefits of RNs performing annual wellness visits (AWVs) for Medicare patients. Having RNs conduct AWVs can decrease the workload of primary care providers, improve patient outcomes, contribute to the revenue of the practice, and elevate nursing standards in the ambulatory setting.
A review on composite materials based on recycled thermoplastics and glass fibres (2011)

The purpose of this paper is to review recent work on composite materials based on recycled thermoplastics and glass fibres (GFs). The high collection and separation cost of plastics waste, and the legislative push to increase recycling rates, require the inclusion of increasing proportions of low‐quality plastic waste into recycled products. A robust method for upgrading mixed plastics recyclates is the incorporation of fillers and reinforcements. In particular, addition of chopped GF can lead to material systems with more favourable and consistent sets of mechanical properties. Provided a good interfacial adhesion is achieved, the key structural properties of the composite (stiffness and strength) are mainly dictated by the reinforcement. Therefore, a wide range of polymers, including blends, are accessible for recycling into semistructural products. Glass fibres are one of the most cost‐effective ways of reinforcing recycled polymers, as testified by several patents and commercial products which appeared in the last decade.
Targeted inversion and reversion of the blood coagulation factor 8 gene in human iPS cells using TALENs (2014)

Hemophilia A, one of the most common genetic bleeding disorders, is caused by various mutations in the blood coagulation factor VIII (F8) gene. Among the genotypes that result in hemophilia A, two different types of chromosomal inversions that involve a portion of the F8 gene are most frequent, accounting for almost half of all severe hemophilia A cases. In this study, we used a transcription activator-like effector nuclease (TALEN) pair to invert a 140-kbp chromosomal segment that spans the portion of the F8 gene in human induced pluripotent stem cells (iPSCs) to create a hemophilia A model cell line. In addition, we reverted the inverted segment back to its normal orientation in the hemophilia model iPSCs using the same TALEN pair. Importantly, we detected the F8 mRNA in cells derived from the reverted iPSCs lines, but not in those derived from the clones with the inverted segment. Thus, we showed that TALENs can be used both for creating disease models associated with chromosomal rearrangements in iPSCs and for correcting genetic defects caused by chromosomal inversions. This strategy provides an iPSC-based novel therapeutic option for the treatment of hemophilia A and other genetic diseases caused by chromosomal inversions.
Grasping Without Squeezing: Design and Modeling of Shear-Activated Grippers (2017)

Grasping objects that are too large to envelop is traditionally achieved using friction that is activated by squeezing. We present a family of shear-activated grippers that can grasp such objects without the need to squeeze. When a shear force is applied to the gecko-inspired material in our grippers, adhesion is turned on; this adhesion in turn results in adhesion-controlled friction, a friction force that depends on adhesion rather than a squeezing normal force. Removal of the shear force eliminates adhesion, allowing easy release of an object. A compliant shear-activated gripper without active sensing and control can use the same light touch to lift objects that are soft, brittle, fragile, light, or very heavy. We present three grippers, the first two designed for curved objects, and the third for nearly any shape. Simple models describe the grasping process, and empirical results verify the models. The grippers are demonstrated on objects with a variety of shapes, materials, sizes, and weights.
Advances in terahertz quantum-cascade lasers using metal-metal waveguides (2005)

We report the progress of terahertz quantum cascade lasers that use resonant-phonon depopulation, including the fabrication of metal-metal waveguides using Cu-Cu thermocompression wafer bonding to extend cw operation up to a temperature of 97 K.
TrustedDB: A Trusted Hardware-Based Database with Privacy and Data Confidentiality (2013)

Traditionally, as soon as confidentiality becomes a concern, data are encrypted before outsourcing to a service provider. Any software-based cryptographic constructs then deployed, for server-side query processing on the encrypted data, inherently limit query expressiveness. Here, we introduce TrustedDB, an outsourced database prototype that allows clients to execute SQL queries with privacy and under regulatory compliance constraints by leveraging server-hosted, tamper-proof trusted hardware in critical query processing stages, thereby removing any limitations on the type of supported queries. Despite the cost overhead and performance limitations of trusted hardware, we show that the costs per query are orders of magnitude lower than any (existing or) potential future software-only mechanisms. TrustedDB is built and runs on actual hardware, and its performance and costs are evaluated here.
Post translational activation of latent transforming growth factor beta (L-TGF-beta): clinical implications. (2001)

Transforming growth factor-betas (TGF-betas) are multifunctional cytokines that exist in 3 isoforms in mammals. The TGF-betas are ubiquitously expressed and all isoforms are secreted as biologically inactive precursors called latent TGF-beta (L-TGF-beta). L-TGF-betas are generally not effective molecules because they are unable to interact with their receptors. However, the removal of or conformational change of the precursor protein called the latency associated peptide (LAP) results in the generation of biologically active TGF-beta. In vitro active TGF-beta has many biological effects but from a clinical point of view one of the most recognized associations of aberrant TGF-beta production is with diseases characterized by enhanced connective tissue synthesis. Recently a number of observations in the context of fibrotic disorders suggest mechanisms of activation of L-TGF-beta1 in vivo. The recognition of mechanisms that activate L-TGF-beta1 in vivo offers the possibility of interfering with the activation of L-TGF-beta1 for therapeutic purposes.
A Recurrent and Compositional Model for Personality Trait Recognition from Short Texts. (2016)

Many methods have been used to recognise author personality traits from text, typically combining linguistic feature engineering with shallow learning models, e.g. linear regression or Support Vector Machines. This work uses deep-learning-based models and atomic features of text, the characters, to build hierarchical, vectorial word and sentence representations for trait inference. This method, applied to a corpus of tweets, shows state-of-the-art performance across five traits compared with prior work. The results, supported by preliminary visualisation work, are encouraging for the ability to detect complex human traits.
Isolation and Purification of Ascorbate Oxidase from<i>Acremonium</i>sp. HI-25 (1992)

A screening test was undertaken to isolate microorganisms that produced ascorbate oxidase. The enzyme activity was found in a culture filtrate of a fungal strain (HI-25), newly isolated from a soil sample. Based on the morphological characteristics, this isolate was identified as Acremonium sp. From the examinations of cultural conditions, optimum conditions for enzyme production were found; strain HI-25 was aerobically cultured by a jar fermenter at 25°C in a medium containing 5% glycerol, 2% defatted soybeans, 0.1% monosodium L-glutamate, 0.1% KH2PO4, 0.02% MgSO4 ·7H2O, and 0.01% KCl, pH 6.0. After cultivation, an ascorbate oxidase was purified from the culture filtrate by an ammonium sulfate fractionation, column chromatographies on DEAE-cellulose and Butyl-Toyopearl, and gel filtration twice on Sephadex G-100. The purification was 850-fold with an activity yield of 8.8%. The purified enzyme gave a single band on SDS polyacrylamide gel electrophoresis, and had a molecular weight of 80,000 by SDS polyacrylamide gel electrophoresis and 76,000 by native gel filtration. This enzyme was most active at pH 4.0, 45°C, and was most stable between pH 6.0-10.0 and at temperatures below 60°C.