gebhart/patrepeval huggingface dataset:
Rapid Ex Vivo Expansion of CMV Specific CTL Suitable for Clinical Immunotherapy Using CpG-DNA Matured PBMC Infected with Recombinant MVA. (2004)

Abstract Adoptive transfer of ex vivo expanded CMV-specific T cells is an effective approach, and an attractive alternative to using anti-virals to manage CMV infection for HSCT recipients. We recently published a robust approach to expanding CMV-specific CTL based on infection of autologous EBV-LCL with the attenuated poxvirus, Modified Vaccinia Ankara (MVA), expressing CMV pp65, pp150, and IE1 proteins. This approach causes vigorous, up to 500fold expansions in as little as 12–14 days of memory CD8+ T cells specific for these immunodominant antigens. In order to improve the specificity of the expanded T cells, a method was sought to derive effective antigen presenting cells (APC) that avoided the use of EBV-LCL. Of equal importance is to develop an expansion approach that avoids the need to involve virally infected APC in developing a clinical product. Our preliminary observation is that rMVA can infect PBMC in vitro, causing high levels of expression of recombinant CMV antigens. To be permissible for high level expression from rMVA, fresh PBMC were treated with different combinations of single-stranded CpG-containing phosphorothioate backbone oligonucleotides (ODN). A three-day incubation with a combination of two ODN (ODN # 2006 and 2216) which are known to stimulate both plasmacytoid dendritic and B-cells were found to reproducibly generate a highly rMVA infectable population of PBMC. In all five healthy CMV-positive donors tested, CpG ODN treated autologous PBMC, infected with recombinant rMVA, elicited a 20-fold average expansion of CMV-specific CD8+ T cells, in 10 days. Several different rMVA expressing CMV genes were evaluated, including a novel vector expressing the UL44 gene product, an immunodominant target of the host cellular immune response. The expanded T cell populations showed minimal alloreactivity, and exhibited high levels of CMV-specific MHC Class I tetramer binding, epitope-specific cytokine production, and cytotoxic activity. The availability of a source of autologous professional APC that can be used after only 3 days of priming, enhances the attractiveness of using rMVA for adoptive immunotherapy for HSCT recipients or donor vaccination.
Greystar: fast and accurate detection of SMS spam numbers in large cellular networks using grey phone space (2013)

In this paper, we present the design of Greystar, an innovative defense system for combating the growing SMS spam traffic in cellular networks. By exploiting the fact that most SMS spammers select targets randomly from the finite phone number space, Greystar monitors phone numbers from the grey phone space (which are associated with data only devices like laptop data cards and machine-to-machine communication devices like electricity meters) and employs a novel statistical model to detect spam numbers based on their footprints on the grey phone space. Evaluation using five month SMS call detail records from a large US cellular carrier shows that Greystar can detect thousands of spam numbers each month with very few false alarms and 15% of the detected spam numbers have never been reported by spam recipients. Moreover, Greystar is much faster in detecting SMS spam than existing victim spam reports, reducing spam traffic by 75% during peak hours.
3708 Study of a Motion Simulator for Artificial Knee Joint Evaluation (2008)

A purpose of this study is to examine the effect that quadriceps femoris force gives to joint reaction force and rotation angle of total knee prosthesis during deep knee flexion such as an unique sitting style called "seiza" in Japanese. For the evaluation, we developed the knee motion simulator which could bend to 180 degrees continually simulating the passive flexion motion performed by clinicians. A new type of total knee prosthesis which could bend to 180 degrees is inserted into bone model, and pulled by quadriceps femoris in three kinds of force vector. The results obtained in this study were almost similar to those of the cadaveric experiment. It is suggested that the joint reaction force and rotation angle of total knee prosthesis are affected by shape of prosthesis, a vector of quadriceps femoris force, and bony aliments during deep knee flexion.
Sigma-delta techniques for integrated test and measurement (2002)

An area efficient and robust integrated test core for analog and mixed-signal circuits is described. The core consists of two signal generators that approximate the output of a sigma-delta modulator using finite repetitious bit patterns. When these structures are coupled with simple filtering techniques and using minimal analog components, the resulting system is capable of performing many measurement functions typical of spectrum analyzers, multimeters, and oscilloscopes.
Development of a double-clad photonic-crystal-fiber-based scanning microscope (2005)

Despite the fact that laser scanning confocal microscopy (LSCM) has become an important tool in modern biological laboratories, it is bulky, inflexible and has limited field of view, thus limiting its applications. To overcome these drawbacks, we report the development of a compact dual-clad photonic-crystal-fiber (DCPCF) based multiphoton scanning microscope. In this novel microscope, beam-scanning is achieved by directly scanning an optical fiber, in contrast to conventional beam scanning achieved by varying the incident angle of a laser beam at an objective entrance pupil. The fiber delivers femtosecond laser pulses for two-photon excitation and collects fluorescence back through the same fiber. Conventional fibers, either single-mode fiber (SMF) or multimode fiber (MMF), are not suitable for this detection configuration because of the low collection efficiency for a SMF and low excitation rate for a MMF. Our newly invented DCPCF allows one to optimize collection and excitation efficiency at the same time. In addition, when a gradient-index (GRIN) lens is used to focus the fiber output to a tight spot, the fluorescence signal collected back through the GRIN lens forms a large spot at the fiber tip because of the chromatic aberrations of the GRIN lens. This problem prevents a standard fiber from being applicable, but is completely overcome by the DCPCF. We demonstrate that this next generation scanning confocal microscope has an extremely simple structure and a number of unique features owing to its fundamentally different scanning mechanism: high flexibility, arbitrarily large scan range, aberration-free imaging, and low cost.
Simultaneous Diagnosis of Severity and Features of Diabetic Retinopathy in Fundus Photography Using Deep Learning (2020)

Deep learning methods for diabetic retinopathy (DR) diagnosis are usually criticized as being lack of interpretability in the diagnostic result, thus limiting their application in clinic. Simultaneous prediction of DR related features during the DR severity diagnosis is able to resolve this issue by providing supporting evidence (i.e. DR related features) for the diagnostic result (i.e. DR severity). In this study, we propose a hierarchical multi-task deep learning framework for simultaneous diagnosis of DR severity and DR related features in fundus images. A hierarchical structure is introduced to incorporate the casual relationship between DR related features and DR severity levels. In the experiments, the proposed approach was evaluated on two independent testing sets using quadratic weighted Cohen's kappa coefficient, receiver operating characteristic analysis, and precision-recall analysis. A grader study was also conducted to compare the performance of the proposed approach with those of general ophthalmologists with different levels of experience. The results demonstrate that the proposed approach could improve the performance for both DR severity diagnosis and DR related feature detection when comparing with the traditional deep learning-based methods. It achieves performance close to general ophthalmologists with five years of experience when diagnosing DR severity levels, and general ophthalmologists with ten years of experience for referable DR detection.
Delayed symptom onset and increased life expectancy in Sandhoff disease mice treated with <i>N</i> -butyldeoxynojirimycin (1999)

Sandhoff disease is a neurodegenerative disorder resulting from the autosomal recessive inheritance of mutations in the HEXB gene, which encodes the beta-subunit of beta-hexosaminidase. GM2 ganglioside fails to be degraded and accumulates within lysosomes in cells of the periphery and the central nervous system (CNS). There are currently no therapies for the glycosphingolipid lysosomal storage diseases that involve CNS pathology, including the GM2 gangliosidoses. One strategy for treating this and related diseases is substrate deprivation. This would utilize an inhibitor of glycosphingolipid biosynthesis to balance synthesis with the impaired rate of catabolism, thus preventing storage. One such inhibitor is N-butyldeoxynojirimycin, which currently is in clinical trials for the potential treatment of type 1 Gaucher disease, a related disease that involves glycosphingolipid storage in peripheral tissues, but not in the CNS. In this study, we have evaluated whether this drug also could be applied to the treatment of diseases with CNS storage and pathology. We therefore have treated a mouse model of Sandhoff disease with the inhibitor N-butyldeoxynojirimycin. The treated mice have delayed symptom onset, reduced storage in the brain and peripheral tissues, and increased life expectancy. Substrate deprivation therefore offers a potentially general therapy for this family of lysosomal storage diseases, including those with CNS disease.
HyComp (2015)

Proposed cache compression schemes make design-time assumptions on value locality to reduce decompression latency. For example, some schemes assume that common values are spatially close whereas other schemes assume that null blocks are common. Most schemes, however, assume that value locality is best exploited by fixed-size data types (e.g., 32-bit integers). This assumption falls short when other data types, such as floating-point numbers, are common. This paper makes two contributions. First, HyComp -- a hybrid cache compression scheme -- selects the best-performing compression scheme, based on heuristics that predict data types. Data types considered are pointers, integers, floating-point numbers and the special (and trivial) case of null blocks. Second, this paper contributes with a compression method that exploits value locality in data types with predefined semantic value fields, e.g., as in the exponent and the mantissa in floating-point numbers. We show that HyComp, augmented with the proposed floating-point-number compression method, offers superior performance in comparison with prior art.
Discovery, Engineering and Pre-Clinical Evaluation of an Anti-vWF A1 Domain Aptamer. (2006)

Abstract Following coronary artery damage, von Willebrand Factor (vWF) multimers adhere to exposed collagen via the vWF A3 domain. Platelets passing through damaged vessels under the conditions of high shear force associated with stenosed arteries will adhere to the vWF A1 domain via GPIb receptors. This initial platelet binding event triggers the formation of a thrombus that can limit blood flow to the myocardium and produce the symptoms of acute coronary syndrome (ACS). Through in vitro selection (SELEX) and subsequent lead optimization, we have generated a nuclease-resistant DNA aptamer that binds to the vWF A1 domain with high affinity. The anti-vWF aptamer inhibits both botrocetin-induced platelet aggregation of platelet rich plasma and shear force-induced platelet aggregation in citrated whole blood. Continuous intravenous infusion of the aptamer in cynomolgus macaques is able to effectively inhibit thrombus formation induced by electrical injury to the carotid artery. Modest effects on template bleeding are observed with the vWF-specific aptamer relative to anti-GPIIb/IIIa therapy. Based upon these results, this aptamer is an excellent candidate for treatment of ACS.
Noninvasive Estimation of Blood Alcohol Concentrations: Ethanol Vapor Above the Eye (1988)

The present study describes, in animals, a novel approach to the in vivo, noninvasive determination of alcohol in the body. The concentration of ethanol in vapor above the lacrimal fluid in the eye was analyzed in situ by the use of a fast (1-min) gas sensor method developed previously for biological liquids. After an oral dose of 1 g/kg to 11 animals, eye vapor measurements and blood samples were obtained over 4 hr. The correlation of 61 blood ethanol concentrations obtained by the two methods yielded a correlation coefficient of 0.92 and a slope of 0.99. The metabolic rates of ethanol determined by gas chromatographic analysis of blood and by ethanol eye vapor analysis are virtually identical. The data suggest that ethanol eye vapor analysis may be an attractive, noninvasive method for the determination of ethanol in animals. The method is not subject to false high readings due to alcohol in the buccal cavity and thus might constitute an alternative to breath analysis in the human. In a separate series, ethanol was determined by head space gas chromatography in samples of blood and lacrimal fluid while the animals were under ketamine anesthesia. The correlation of ethanol concentrations in blood and lacrimal fluid (r = 0.99) shows that ethanol is distributed in lacrimal fluid which comprises part of total body water.
A novel reconfigurable origami accordion antenna (2014)

An accordion origami structure is presented, which can be expanded and collapsed. Based on this structure, one origami antenna is designed with operating frequency that can be changed based on its height. A prototype antenna model is manufactured to validate the simulation model. The return loss, far-field radiation pattern and peak gain of this antenna are reported using simulations and measurements.
Parallel Algorithm for Single-Source Earliest-Arrival Problem in Temporal Graphs (2017)

Many real-world networks, including online social networks and communication networks, are commonly modeled as temporal graphs. Answering earliest-arrival queries in temporal graphs is one of the most fundamental studies with numerous applications, such as information diffusion and measuring temporal closeness centrality. As graph sizes are growing rapidly, speedup of query execution time becomes even more important.In this paper, we propose a novel edge-centric parallel algorithm for solving single-source earliest-arrival problem in temporal graphs based on a new data structure named Edge-Scan-Dependency Graph (ESD-Graph). We evaluate the proposed parallel algorithm by theoretical analysis as well as by empirical experiments on real-world temporal graphs and synthetic graphs. Empirical results show that the new parallel algorithm outperforms the existing serial algorithm by up to 8.2 and 9.5 times on multi-core processors for real-world data and synthetic data respectively.
THE DEHYDRATION OF THE LOWER FATTY ACIDS BY EXTRACTIVE DISTILLATION (1990)

Abstract The lower fatty acids cannot be readily separated from water by distillation. Formic acid forms a maximum azeotrope with water, acetic acid boils very close to water and propionic acid forms a minimum azeotrope with water. These acids can be easily separated from water by extractive distillation. Effective agents for each dehydration are presented.
Fast approximate answers to aggregate queries on a data cube (2003)

Modern decision support systems require very quick (interactive) responses from the DBMS, but pose complex queries on large volumes of data. In this paper, we present a novel solution to this problem: we precompute concise histogram statistics on the data to answer the queries quickly but approximately. Our hypothesis is that many decision support applications can tolerate small errors in query results in return for large reductions in response times. In particular, we propose the use of multiple histograms to approximate the data cube and answer aggregate queries approximately using this summarized data. We enhance histograms to estimate the quality of the approximate answers. We primarily explore the interaction among various histograms on the data cube in order to minimize the space needed when an upper bound on the errors is given. Our main contribution in this paper is an efficient technique for selecting a provably near-optimal set of histograms on the data cube. Extensive experiments show that our technique results in very accurate and concise statistics. Our technique is general in nature and can also be used for selecting a set of histograms (or other statistics) on a relation for the purpose of selectivity estimation.
Touch Mode Capacitive Pressure Sensor (2003)

Touch Mode Capacitive Pressure Sensor proposed by Ko, et al. has great potentialities for several applications, because of its high sensitivity, good linearity and low temperature depend- ence of electrical performances 1) . We have been developed the Touch Mode Capacitive Pressure Sensor for a tire pressure monitoring system, which can be implanted in tire rubber through its curing process. The sensor needs high burst pressure greater than 3.5MPa, which is the maximum pressure during the process. The purpose of this work is to find the cause of diaphragm breakage in lower applied pressure, and to obtain the sensors that have the required minimum burst pressure for the tire pressure monitoring system. We especially focused on sur- face morphology of diaphragm and found that etch-pits on the diaphragm made the strength weak. The etch-pits were generated during etching process to form the diaphragm. Finally, we could improve the etching process and develop the Touch Mode Capacitive Pressure Sensor, which had a high burst pressure enough to be applied in the tire pressure monitoring system.
MicroRNA-122 antagonism against hepatitis C virus genotypes 1–6 and reduced efficacy by host RNA insertion or mutations in the HCV 5′ UTR (2011)

MicroRNA-122 (miR-122) is believed to stimulate hepatitis C virus (HCV) replication through interaction with two adjacent sites downstream of stem loop I (SLI) within the HCV 5' untranslated region (5' UTR). Recently, it was demonstrated that locked nucleic acid SPC3649-induced miR-122 antagonism suppressed HCV genotype 1a and 1b infection in vivo. However, virus-producing culture systems with 5' UTR of different HCV genotypes have not been available for testing 5' UTR-based treatment approaches. Using JFH1-based Core-NS2 genotype recombinants, we developed 5' UTR-NS2 recombinants of HCV genotypes 1a, 1b, 2a, 2b, 3a, 4a, 5a, and 6a with efficient growth in Huh7.5 cells. Deletion mutagenesis studies demonstrated that the 5' UTR SLI was essential for genotypes 1-6 infection. However, lack of SLI could be compensated for by insertion of other structured HCV or host RNA sequences, including U3 small nucleolar RNA. We demonstrated that SPC3649-induced miR-122 antagonism had a potent antiviral effect against HCV genotypes 1-6 5' UTR-NS2 viruses. Strikingly, HCV recombinant virus with substitution of SLI and miR-122 binding site 1 (S1) by the U3 RNA sequence was not affected by miR-122 antagonism; this was attributed to the lack of an intact S1 by reverse genetics studies. Therefore, we engineered the corresponding U3 RNA sequences into S1 and demonstrated that HCV recombinants with wild-type SLI and single or combined mutations at four of eight nucleotides of S1 were viable in Huh7.5 cells. These mutations reduced the efficacy of SPC3649 treatment, indicating that escape variants to miR-122 antagonism-based HCV therapy could potentially occur.
Implementation of high-dynamic range pixel architecture for SWIR applications (2014)

This paper presents novel unit cell architecture for short wave infrared (SWIR) imaging applications. It has two input stages which are CTIA and SFD covering for both respectively low and high light, levels and automatic input stage selection circuitry that chooses best input stage. User can select 2 modes for FPA manual and automatic mode. In manual mode, user can set CTIA or SFD for all pixels according to user needs. In automatic mode, each pixel selects input stage itself according to light level. Light level threshold can be adjusted with reference voltage. Automatic input stage selection for each pixel brings high SNR level and low noise along with highest possible dynamic range for SWIR imaging applications. CMOS 0.18μm technology is used to realize unit cell. In the architecture of unit cell, circuit level techniques are used to optimize layout size.
Contact Analysis of Non-Gaussian Surfaces for Minimum Static and Kinetic Friction and Wear (1996)

Most statistical contact analyses assume that surface heights and peak (summit) height distributions follow a Gaussian distribution. However, engineering surfaces are frequently non-Gaussian with a degree of non-Gaussian character dependent upon materials and surface finishing processes used. For example, magnetic rigid disk surfaces used in magnetic storage industry are highly non Gaussian. The use of a Gaussian analysis in such cases can lead to erroneous results. This study for the first time presents a method to carry out a statistical analysis of non-Gaussian surfaces. Real area of contact, number of contacts, contact pressure and meniscus force (in wet interfaces) are calculated for probability density functions having different skewness and kurtosis. From these curves, the optimum value of skewness and kurtosis can be predicted for minimum static/kinetic friction. It is found that a range of positive skewness (between 0.3–0.7) and a high kurtosis (greater than five) significantly lower the real area of contact and meniscus contribution implying low friction and wear. Also, sensitivity of film thickness to static friction goes down for a surface with a positive skewness and a high kurtosis.
[Properties of interleukin 8 and its correlation with inflammatory diseases and malignant neoplasia]. (1994)

Inflammation is a vital consequence of tissue injury caused by various reasons including invasion of foreign particles, infection with microorganisms, autoimmune responses, ischemia-reperfusion injury, and malignant neoplasia. In 1987, a major neutrophil chemotactic and activating factor, now called interleukin 8 (IL-8), was purified and molecularly cloned. In this article, general overview of IL-8 was made describing biochemical structure, regulation of production of IL-8, properties of the receptors for IL-8 and pathophysiological roles of IL-8 in inflammation.