gebhart/patrepeval huggingface dataset:
Design analysis for refolding monomeric protein (1997)

Abstract Renaturation of protein expressed as inclusion bodies within Escherichia coli is a key step in many bioprocesses. Operating conditions for the refolding step dramatically affect the amount of protein product recoverd, and hence profoundly influence the process economics. The first systematic comparison of refolding conducted in batch, fed‐batch and continuous stirred‐tank reactors is provided. Refolding is modeled as kinetic competition between first‐order refolding (equilibrium reaction) and irreversible aggregation (second‐order). Simulations presented allow direct comparison between different flowsheets and refolding schemes using a dimensionless economic objective. As expected from examination of the reaction kinetics, batch operation is the most inefficient mode. For the base process considered, the overall cost of fed‐batch and continuous refolding is virtually identical (less than half than of the batch process). Reactor selection and optimization of refolding using overall economics are demonstrated to be vitally important.
Experimental results of an inexpensive short baseline acoustic positioning system for AUV navigation (2002)

The Ocean Engineering Department at Florida Atlantic University (FAU) and the Naval Postgraduate have implemented AUV acoustic positioning systems based on the Desert Star Dive Tracker short base line (SBL) system. The motivation for these implementation efforts was to develop a short range, inexpensive, but relatively high precision navigation system for each institution's autonomous underwater vehicles (AUVs). In the NPS case the SBL system is to support navigation of their Phoenix vehicle in mine reconnaissance type missions where the vehicle needs to more precisely locate obstacles and mine like objects. In the FAU case the SBL system is to support guidance of the Ocean Explorer (OEX) vehicle in rendezvous and docking maneuvers. The Dive Tracker SBL can produce supposed positioning accuracies on the order of 6 rms. FAU has build a portable easily deployed collapsible frame for supporting 3 SBL transducers or beacons. The frame is arranged in star that is anchored to the bottom. The baseline between transducers is approximately 20 ft. The OEX carries a transducer and electronics that compute its position relative to the baseline. A docking target placed near the baseline and anchored to the bottom. The location of the target is then determined by manually positioning the AUV transducer at the target and taking SBL fixes, A series of at-sea tests of this system have been conducted. This paper presents results of the at sea tests of this positioning system off the coast of Boca Raton Florida. The analysis will comment on the efficacy of this type of system for guidance of small AUVs in docking maneuvers.
A provenance-aware virtual sensor system using the Open Provenance Model (2010)

Sensor web applications such as real-time environmental decision support systems require the use of sensors from multiple heterogeneous sources for purposes beyond the scope of the original sensor design and deployment. In such cyberenvironments, provenance plays a critical role as it enables users to understand, verify, reproduce, and ascertain the quality of derived data products. Such capabilities are yet to be developed in many sensor web enablement (SWE) applications. This paper develops a provenance-aware “Virtual Sensor” system, where a new persistent live “virtual” sensor is re-published in realtime after some model-based computational transformations of the raw sensor data streams. We describe the underlying OPM (Open Provenance Model) API's (Application Programming Interfaces), architecture for provenance capture, creation of the provenance graph and publishing of the provenance-aware virtual sensor where the new virtual sensor time-series data is augmented with OPM-compliant provenance information. A case study on creating real-time provenance-aware virtual rainfall sensors is illustrated. Such a provenance-aware virtual sensor system allows digital preservation and verification of the new virtual sensors.
Patient Characteristics Compete with Dose as Predictors of Acute Treatment Toxicity in Early Phase Clinical Trials (2004)

Independent of dose, certain pretreatment characteristics, including measures of organ function that are in the normal range, were found to be predictors of treatment toxicity. Because of its sensitivity to differences in overall toxicity, the TI should prove to be a useful tool for identifying predictors of chemotherapy-related toxicity.
직업재활시설 슈퍼비전에 관한 질적 사례연구 - M직업재활센터 사례를 중심으로 - (2018)

본 연구는 직업재활시설 슈퍼비전의 체계 정립에 대한 제언을 목적으로, 문헌연구를 통해 직업재활시설이 성공적인 기능과 역할을 수행하기 위해 어떻게 슈퍼비전의 체계를 정립해 나가야 할 것인지 살펴보았다. 이를 위해 직업재활시설 중에서 슈퍼비전의 체계를 마련하고 실천하고 있는 M직업재활센터의 2016년부터 2018년까지의 슈퍼비전 기록을 분석하고 종사자 4명을 대상으로 한 1대 1 인터뷰를 분석하였다. 그 결과, 직업재활시설은 생산성과 복지성이라는 두 마리 토끼를 잡아야하는 독특한 특성을 갖는 복지기관으로서 인력, 재정, 제도적 상황에서 무형식 슈퍼비전을 수행할 수 밖에 없으며, 내용에 있어서도 교육, 고용, 복지라는 세 영역을 통합적으로 질서정연하게 결합할 수 있도록 종사자들의 역량을 강화할 수 있는 슈퍼비전이 필요함을 확인하였다. 그러나 현실적으로 재정적, 인력적, 제도적 문제로 인해 슈퍼바이저와 슈퍼바이지 모두 소진되어 있는 것으로 나타났다. 그럼에도 불구하고, 지지적 슈퍼비전은 직무만족도를 높이고 역량강화에 도움을 주는 것으로 나타났다. 본 연구는 직업재활시설 슈퍼비전에 대한 연구가 전무한 상황에서 사례를 통해 직업재활시설의 슈퍼비전 체계 정립의 필요성을 제시하였다는 점에서 의의가 있다.
ChemInform Abstract: CYCLODIENONES. 9. REACTION OF 4‐HALO‐2,4,6‐TRI‐TERT‐BUTYL‐2,5‐CYCLOHEXADIEN‐1‐ONES WITH PYRAZOLES AND PREPARATION OF 1‐(2‐HYDROXYPHENYL)‐ AND 1‐(4‐HYDROXYPHENYL)PYRAZOLES (1982)

Abstract Aus den Halogencyclohexadienonen (I) erhält man mit den Pyrazolen (II) Produktgemische aus den Cyclohexadienonen (III) und (X), Phenolen (IV) bis (VIII) und demChinon (IX).
Naked-Eye Sensitive Detection of Immunoglubulin G by Enlargement of Au Nanoparticles In Vitro This research was financed by grants from the National Natural Science Foundation of China (NSFC) and Tsinghua University. (2002)

Wachsendes Gold: Nach Vergrößerung von Au-Nanopartikeln in vitro mit HAuCl4/NH2OH⋅HCl und damit verbundener Immobilisierung auf Nitrocellulosefiltern lässt sich humanes Immunglobulin G (h-IgG) mit bloßem Auge erkennen (siehe Schema; •=Au-Nanopartikel, ⧫=anti-h-IgG, Y=h-IgG). Bei einer Detektionsgrenze von etwa 10 pg mL−1 nimmt die Methode es mit Verfahren auf, die auf Fluoreszenz, Radioaktivität oder enzymatischen Farbreaktionen basieren.
Reconstitution of dinucleosomes on restriction fragments (1983)

Conference Article| August 01 1983 Reconstitution of dinucleosomes on restriction fragments KEITH J. JOHNSON; KEITH J. JOHNSON 1Department of Biochemistry, St. Mary's Hospital Medical School, Praed Street, Paddington, London W2 1PG, U.K. Search for other works by this author on: This Site PubMed Google Scholar ALAN D. B. MALCOLM ALAN D. B. MALCOLM 2Department of Biochemistry, St. Mary's Hospital Medical School, Praed Street, Paddington, London W2 1PG, U.K. Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1983) 11 (4): 370. https://doi.org/10.1042/bst0110370 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter LinkedIn Cite Icon Cite Get Permissions Citation KEITH J. JOHNSON, ALAN D. B. MALCOLM; Reconstitution of dinucleosomes on restriction fragments. Biochem Soc Trans 1 August 1983; 11 (4): 370. doi: https://doi.org/10.1042/bst0110370 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search This content is only available as a PDF. © 1983 Biochemical Society1983 Article PDF first page preview Close Modal You do not currently have access to this content.
Lessons from Fragile X Regarding Neurobiology, Autism, and Neurodegeneration (2006)

The fragile X mental retardation 1 gene (FMR1) mutation causes two disorders: fragile X syndrome (FXS) in those with the full mutation and the fragile X-associated tremor/ataxia syndrome (FXTAS) in some older individuals with the premutation. FXS is caused by a deficiency of the FMR1 protein (FMRP) leading to dysregulation of many genes that create a phenotype with ADHD, anxiety, and autism. FXTAS is caused by the elevation of FMR1-mRNA to levels 2 to 8 times normal in the premutation. This causes an RNA gain of function toxicity leading to brain atrophy, white matter disease, neuronal and astrocytic inclusion formation, and subsequent ataxia, intention tremor, peripheral neuropathy, and cognitive decline. The neurobiology and pathophysiology of FXS and FXTAS are described in detail.
Microstructural analysis of oxide layer formation in ferritic stainless steel interconnects (2011)

The oxide layer formation of ferritic stainless steel (Crofer22APU) oxidized in an air furnace at 800°C was analysed by transmission electron microscopy (TEM) and electron back scattered diffraction (EBSD).EBSD analysis revealed that crystallographic orientation affects the oxidation behaviour of Crofer22APU. On {110} grains, most oxide granules had the same orientation but on {111} grains, they had random orientation.TEM analysis revealed that the oxide layer consisted of spinel- chromia-spinel multi layered structure on the {110} matrix grain but no sub spinel oxide layer on the {111} matrix grain. On the {110} closed packed plane of matrix, the chromia oxide formation with the <110> direction of rhombohedral structure on the {001} closed packed plane parallels the <001> direction on the {110} plane of BCC matrix was observed, which is known as Pitsch-Schrader orientation relationship. On the {111} plane grain, there was no specific orientation relationship. Energy dispersive spectroscopy (EDS) analysis in TEM showed that an Mn rich region was developed on the {110} grain beneath chromia oxide and the conversion of this region to (Cr,Mn)3O4 spinel oxide layer causes multi layered oxide structures on the {110} matrix grain.
Engagement of ICAM-3 Provides a Costimulatory Signal for Human Immunodeficiency Virus Type 1 Replication in both Activated and Quiescent CD4<sup>+</sup>T Lymphocytes: Implications for Virus Pathogenesis (2004)

Human immunodeficiency virus type 1 (HIV-1) replication is regulated by several extracellular signals. We demonstrate that intercellular adhesion molecule 3 (ICAM-3) acts as a costimulating molecule to increase HIV-1 transcription and viral production, a process allowing productive infection of quiescent CD4+ T lymphocytes. The present work suggests an important role for ICAM-3 in HIV-1 replication.
Host Amplification in a Dithioacetal-Based Dynamic Covalent Library (2017)

Molecular amplification in a dithioacetal-based dynamic library is described for the first time. The homatropine induced selection, amplification, and isolation of one cyclophane host demonstrates the utility of dithioacetal exchange for preparing responsive dynamic libraries. Nuclear magnetic resonance and isothermal titration calorimetry analysis suggest that the amplified macrocycle forms a 1:1 complex with the template. This is the first report about a host/guest system involving a dithioacetal cyclophane.
A magnetic microchip for controlled transport of attomole levels of proteins (2009)

A novel method of controlled transport of proteins immobilized on micrometre-sized magnetic beads in a lab-on-a-chip environment is presented. Bead motion is controlled by lithographically made magnetic elements forming transportation lines in combination with an applied in-plane rotating magnetic field. In this way, transport of attomole amounts of proteins is controlled with micrometre precision. Also, the activity of proteins immobilized on the beads is demonstrated by injecting antibodies into the system. A critical step in developing the method was to reduce sticking forces between beads and substrate during transportation of proteins. Charge interaction was found to be of minor importance compared to hydrophobic forces. To achieve a reliable transport of biologically active proteins, both substrate and beads were coated with polyethylene glycol (PEG) and the protein covered beads were suspended in buffer with surfactants. The described system fulfils all the important unit operations of a microfluidic platform and, as a further advantage, presents less need for microchannels and electric wiring.
Challenges of heterotopic ossification—Molecular background and current treatment strategies (2018)

Heterotopic ossification (HO) is an abnormal formation of mature lamellar bone within extraskeletal soft tissues, such as muscles, tendons, and ligaments. This process is thought to be induced by inflammation associated with tissue injuries. HO is classified using two subtypes: resulting from injury or genetically inherited. HO formation is associated with polytrauma patients with traumatic brain injuries and spinal cord injuries. Moreover, HO is also considered to be a post-operative risk factor in some orthopaedic procedures. In this review, we summarize our current understanding of the pathology of different types of HO and discuss its current and future therapies. Thus far, research has revealed cellular and molecular pathways leading to HO formation and proposed several possible mechanisms leading to HO and conserved signalling pathways common in the different HO subtypes. Non-steroidal anti-inflammatory drug treatment and localized low-dose irradiation are currently the only available prophylactic treatments for HO. However, they are not always effective and do not target the osteogenic processes directly. New therapeutic strategies targeting the pathological processes of HO, such as bone morphogenetic protein (BMP) inhibitors like noggin, BMP type 1 receptor inhibitor, and nuclear retinoid acid receptor-gamma (RARγ) agonists, are currently being investigated. In-depth understanding of the HO pathological process could help to develop effective therapeutic strategies.
Combined microcrystal and amorphous silicon cells (1982)

Solar cells have been constructed by depositing a microcrystalline p-silicon layer on an amorphous i-n structure. Here p and n denote the type of dopant and i the intrinsic. The silicon layers are formed by electron beam evaporation and doped through the coevaporation of boron for p type and antimony for n type. After hydrogenation by plasma annealing, solar cells are obtained with an open circuit voltage of 540 mV and short circuit current of 5 mA/cm2 under AMl simulated light. The fill factor is 46%.
Highly Sensitive and Specific Detection of Rare Variants in Mixed Viral Populations from Massively Parallel Sequence Data (2012)

Viruses diversify over time within hosts, often undercutting the effectiveness of host defenses and therapeutic interventions. To design successful vaccines and therapeutics, it is critical to better understand viral diversification, including comprehensively characterizing the genetic variants in viral intra-host populations and modeling changes from transmission through the course of infection. Massively parallel sequencing technologies can overcome the cost constraints of older sequencing methods and obtain the high sequence coverage needed to detect rare genetic variants (< 1%) within an infected host, and to assay variants without prior knowledge. Critical to interpreting deep sequence data sets is the ability to distinguish biological variants from process errors with high sensitivity and specificity. To address this challenge, we describe V-Phaser, an algorithm able to recognize rare biological variants in mixed populations. V-Phaser uses covariation (i.e. phasing) between observed variants to increase sensitivity and an expectation maximization algorithm that iteratively recalibrates base quality scores to increase specificity. Overall, V-Phaser achieved > 97% sensitivity and > 97% specificity on control read sets. On data derived from a patient after four years of HIV-1 infection, V-Phaser detected 2,015 variants across the -10 kb genome, including 603 rare variants (< 1% frequency) detected only using phase information. V-Phaser identified variants at frequencies down to 0.2%, comparable to the detection threshold of allele-specific PCR, a method that requires prior knowledge of the variants. The high sensitivity and specificity of V-Phaser enables identifying and tracking changes in low frequency variants in mixed populations such as RNA viruses.
Dual-gate m.e.s.f.e.t. self-oscillating X-band mixers (1979)

GaAs dual-gate m.e.s.f.e.t.s have been successfully used as self-oscillating down-convertors in X-band. A single device replaces the preamplifier, mixer and local oscillator. The best conversion gain achieved by mixing from 10 GHz down to 1 GHz was 12 dB. The input (gate 1) to output (drain) port isolation amounted to 16 dB. Slug-tuner and ‘disc’-resonator circuits were tested and showed comparable gain and noise performance. Best d.s.b. noise figures of 5.5 dB could be realised at an i.f. of 1 GHz with an associated conversion gain of 4 dB.
Cyclic guanidines. VII. Structure-activity relationships of hypoglycemic cyclic guanidines. (1979)

The hypoglycemic activity and physico-chemical properties (pKa, log P) of cyclic guanidines were measured. Qualitative structure-activity relationships were investigated in various mono-, bi-, and tricyclic guanidines and it became apparent that compounds which have a bulky group and a pKa value between 8-11 showed potent activity. It was possible to correlate hypoglycemic activity with the pKa values and partition coefficients of cyclic guanidines.
Dual-element, unshielded hall head (1984)

A new Hall-effect read head, consisting of either one or two thin InSb films perpendicular to the medium surface and incorporating no ferromagnetic materials, is proposed. The predicted performance of this head, obtained by numerical modeling, is better than that of inductive or magnetoresistive film heads, as well as that of ferrite heads. Because of the absence of ferromagnetic materials, this head generates no Barkhausen noise. Using the best presently available materials, its output voltage, signal-to-noise ratio and off-track performance are such that very high track densities - 100 tracks/mm (2500 tpi) and higher - are achievable.
Massenspektrometrische und röntgenstrukturanalytische Untersuchungen an Komplexen des Typs (COD)PtX<sub>2</sub> (X = Cl, Br, I, CH<sub>3</sub>, CH<sub>2</sub>CMe<sub>3</sub>, CH<sub>2</sub>SiMe<sub>3</sub>) (1997)

Abstract Bei massenspektrometrischen Untersuchungen an Komplexen des Typs (COD)PtX 2 X = Cl ( 1 ), Br ( 2 ), I ( 3 ), Me ( 4 ), CH 2 CMe 3 ( 5 ) und CH 2 SiMe 3 ( 6 ), (COD) = 1,5‐Cyclooctadien) werden von allen Verbindungen der Molpeak mit dem charakteristischen Isotopenmuster des Platins bzw. des Platins mit Chlor, Brom oder Silicium gefunden. Die beobachteten Peakgruppen der Verbindungen 1–5 bei 300 m/c deuten auf eine hohe Stabilität des (COD)Pt‐Fragmentes hin. Ein neuer Syntheseweg zur Darstellung von (COD)PtBr 2 ( 2 ) wird beschrieben. Die Verbindungen (COD)Pt(CH 2 CMe 3 ) 2 ( 5 ) und (COD)Pt(CH 2 SiMe 3 ) 2 ( 6 ) wurden röntgenstrukturanalytisch untersucht.
Enantiospecific and stereospecific rhodium(I)-catalyzed carbonylation and ring expansion of aziridines. Asymmetric synthesis of .beta.-lactams and the kinetic resolution of aziridines (1989)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEnantiospecific and stereospecific rhodium(I)-catalyzed carbonylation and ring expansion of aziridines. Asymmetric synthesis of .beta.-lactams and the kinetic resolution of aziridinesSerge Calet, Fabio Urso, and Howard AlperCite this: J. Am. Chem. Soc. 1989, 111, 3, 931–934Publication Date (Print):February 1, 1989Publication History Published online1 May 2002Published inissue 1 February 1989https://pubs.acs.org/doi/10.1021/ja00185a023https://doi.org/10.1021/ja00185a023research-articleACS PublicationsRequest reuse permissionsArticle Views1795Altmetric-Citations166LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Development of a Proline-Based Selection System for Reliable Genetic Engineering in Chinese Hamster Ovary Cells (2020)

Chinese hamster ovary (CHO) cells are the superior host cell culture models used for the bioproduction of therapeutic proteins. One of the prerequisites for bioproduction using CHO cell lines is the need to generate stable CHO cell lines with optimal expression output. Antibiotic selection is commonly employed to isolate and select CHO cell lines with stable expression, despite its potential negative impact on cellular metabolism and expression level. Herein, we present a novel proline-based selection system for the isolation of stable CHO cell lines. The system exploits a dysfunctional proline metabolism pathway in CHO cells by using a pyrroline-5-carboxylate synthase gene as a selection marker, enabling selection to be made using proline-free media. The selection system was demonstrated by expressing green fluorescent protein (GFP) and a monoclonal antibody. When GFP was expressed, more than 90% of stable transfectants were enriched within 2 weeks of the selection period. When a monoclonal antibody was expressed, we achieved comparable titers (3.35 ± 0.47 μg/mL) with G418 and Zeocin-based selections (1.65 ± 0.46 and 2.25 ± 0.07 μg/mL, respectively). We further developed a proline-based coselection by using <i>S. cerevisiae PRO1</i> and <i>PRO2</i> genes as markers, which enables the generation of 99.5% double-transgenic cells. The proline-based selection expands available selection tools and provides an alternative to antibiotic-based selections in CHO cell line development.
Fault current reduction using inverter controlled energy storage for shipboard MVAC power system (2011)

We have shown that an electronically controlled Energy Storage System (ESS), co-located with the main power source in a ship MVAC power system, can be used to absorb part of the fault current so that protective equipment can operate at reduced stress thus prolonging their life and increasing the reliability of the devices. Analysis and simulation results show that the method effectively reduces fault current in a multi-generator power system, but may not be feasible for a shipboard system due to the constraints imposed by cost effectiveness, size, availability of required hardware and energy storage capacity.
Hollow Manganese Oxide Nanoparticles as Multifunctional Agents for Magnetic Resonance Imaging and Drug Delivery (2008)

MRI jack-o'-lanterns? Hollow manganese oxide nanoparticles (HMON) show greatly improved relaxivities and drug-loading capacities compared to those with solid interiors (WMON). Their efficient cellular uptake demonstrates their potential as a bifunctional medical system that combines diagnostic imaging and targeted therapy.
Self-thinning Limits in Two and Three Dimensions (2012)

The principles behind self-thinning laws and stand density management diagrams are examined. Relationships are analyzed based on trajectories of unthinned and thinned stands in a 3-dimensional state space. Limiting self-thinning lines and planes are demonstrated using a dynamic stand growth model for loblolly pine.