gebhart/patrepeval huggingface dataset:
Distinct local immunogenic stimuli dictate differential requirements for CD4<sup>+</sup>and CD8<sup>+</sup>T cell subsets in the pathogenesis of spontaneous autoimmune diabetes (2007)

The strong MHC class II association in human as well as murine Type 1 diabetes (T1D) suggests a central role for CD4+T cells in the disease pathogenesis. Nonetheless, CD8+T cells also play a role in the pathogenic process. We describe how CD4+ or CD8+T cells can contribute differentially to the pathogenesis of T1D using the HLA-DQ8 transgenic mouse models. HLA-DQ8 transgenic mice expressing the costimulatory molecule, B7.1 (RIP.B7.1), or the proinflammatory cytokine, TNF-alpha (RIP.TNF) or both (RIP.B7.RIP.TNF) under the control of rat insulin promoter (RIP) were used. Our observations indicate that in the RIP-B7 model, CD4+T cells were absolutely required for diabetes to occur. However, when CD8+ T cells were also present, the incidence of diabetes increased. On the other hand, in the RIP-TNF model, CD8+T cells were absolutely required for diabetes to occur. Interestingly, when CD4+T cells were also present, the incidence of diabetes decreased. In the RIP-B7.RIP-TNF double transgenic mouse model, either CD4+ or CD8+T cells were sufficient to precipitate diabetes in 100% of the animals. Thus, the relative roles of CD4+ or CD8+T cells in the pathogenesis of T1D are possibly determined by the local inflammatory stimuli.
Factorizing personalized Markov chains for next-basket recommendation (2010)

Recommender systems are an important component of many websites. Two of the most popular approaches are based on matrix factorization (MF) and Markov chains (MC). MF methods learn the general taste of a user by factorizing the matrix over observed user-item preferences. On the other hand, MC methods model sequential behavior by learning a transition graph over items that is used to predict the next action based on the recent actions of a user. In this paper, we present a method bringing both approaches together. Our method is based on personalized transition graphs over underlying Markov chains. That means for each user an own transition matrix is learned - thus in total the method uses a transition cube. As the observations for estimating the transitions are usually very limited, our method factorizes the transition cube with a pairwise interaction model which is a special case of the Tucker Decomposition. We show that our factorized personalized MC (FPMC) model subsumes both a common Markov chain and the normal matrix factorization model. For learning the model parameters, we introduce an adaption of the Bayesian Personalized Ranking (BPR) framework for sequential basket data. Empirically, we show that our FPMC model outperforms both the common matrix factorization and the unpersonalized MC model both learned with and without factorization.
Serum Amyloid A Regulates Granulomatous Inflammation in Sarcoidosis through Toll-like Receptor-2 (2009)

Serum amyloid A is a constituent and innate regulator of granulomatous inflammation in sarcoidosis through Toll-like receptor-2, providing a mechanism for chronic disease and new therapeutic targets.
Functional size measurements of the ubiquinol oxidase activity of the cytochrome <i>o</i> terminal oxidase complex of <i>Escherichia coli</i> (1991)

The functional molecular mass for ubiquinol-1 oxidation by the Escherichia coli terminal oxidase, cytochrome o, was determined by radiation-inactivation analysis of membranes from a cytochrome d-deficient mutant. The functional molecular mass for ubiquinol-l oxidase activity was found to be 38.3 +/- 2.65 kDa.
Formation of Tl-Ca-Ba-Cu-O films by diffusion of Tl into rf-sputtered Ca-Ba-Cu-O (1988)

Using a rf sputtering method with a single Ca-Ba-Cu-O target (Ca:Ba:Cu=2:2:3), thin films with a thickness of about 1 μm have been deposited on ZrO2 and Si substrates. Diffusion experiments showed that the resistivity can be largely reduced by treating the films in an environment containing O2 and Tl. Treatment experiments at different temperatures further showed that the resistivity was affected by the temperature. By treating at 730 °C for about 1 h, a film on ZrO2 with a transition temperature of about 110 K has been obtained. Zero resistance was achieved at 75 K.
STM observation of metal-nonmetal granular thin films (1999)

We have observed scanning tunneling microscopy (STM) topographic images for structural characterization and measured current-voltage (I-V) spectra (scanning tunneling spectroscopy: STS) for the investigation of local transport properties of Co-Al-O granular thin films exhibiting tunnel-type giant magnetoresistance (GMR). STM topographic images indicated round-shaped bright regions and dark channels, which are associated with Co granules and Al-oxide, respectively. From the I-V characteristics the electronic charging effect, i. e., the effect of Coulomb blockade, was confirmed. In addition, the tunneling current varied stepwise as a function of the voltage, suggesting the appearance of a Coulomb staircase.
Interleukin-2-secreting mouse fibroblasts transfected with genomic DNA from murine neoplasms induce tumor-specific immune responses that prolong the lives of tumor-bearing mice. (1995)

Genetic alterations are a common feature of the malignant phenotype. Among other properties, altered genes may be responsible for invasion and metastasis, as well as for resistance to chemotherapeutic agents. Under appropriate circumstances, the products of other altered genes expressed by malignant cells may act as tumor-associated T-cell epitopes, capable of provoking antitumor immune responses. As a novel means of augmenting the immunogenicity of the gene products, unfractionated, sheared genomic DNA from various tumor cell lines (B16F1 melanoma, B16F10 melanoma, MOPC-315 plasmacytoma, C1498 lymphoma, or J558 myeloma), or from non-neoplastic liver cells of tumor-free mice, was transfected into LM cells, a mouse fibroblast cell-line (H-2k) that had been modified previously by retroviral gene transfer to secrete interleukin-2 (IL-2). The IL-2-secreting transfected cell populations were then tested for their immunogenic properties toward B16F1 (H-2b) or C1498 (H-2b) cells in syngeneic C57BL/6 mice. The antitumor responses were specific for the type of tumor from which the DNA was obtained. The survival of C57BL/6 mice injected with a mixture of viable B16F1 cells and IL-2-secreting LM cells transfected with DNA from B16F1 cells was significantly prolonged. In a similar manner, the survival of C57BL/6 mice injected with a mixture of C1498 cells and IL-2-secreting LM cells transfected with DNA from C1498 cells was prolonged as well. The immunity was mediated predominantly by CD8+ and natural killer/lymphokine-activated killer (NK/LAK) cells. These data raise the possibility that a cell line altered previously for cytokine secretion may be readily modified to provide immunologic specificity for the neoplasms of individual cancer patients.
Streamline-Based History Matching: A Review (2007)

In recent years, several methods for streamline-based history matching have been developed. These methods have proved to be efficient for three reasons: First, streamlines delineate flow patterns and can therefore be used to define reduced inverse models. Second, streamline methods provide fast forward simulation. Third, streamline-based sensitivities can be evaluated directly based on one flow simulation for different reservoir responses. We here give a literature review of streamline-based sensitivities and streamline methods used for history matching.\nOppdragsgiver: SINTEF
Bridging the Gap: Ways to Help Children Understand the Elderly (1985)

The following is a miscellaneous collection of ideas for learning activities which can enhance, create, encourage, and develop a "communication bridge" between the elderly and today's youth.
Sclerostin Antibody Therapy for the Treatment of Osteoporosis: Clinical Prospects and Challenges (2016)

It is estimated that over 200 million adults worldwide have osteoporosis, a disease that has increasing socioeconomic impact reflected by unsustainable costs associated with disability, fracture management, hospital stays, and treatment. Existing therapeutic treatments for osteoporosis are associated with a variety of issues relating to use, clinical predictability, and health risks. Consequently, additional novel therapeutic targets are increasingly sought. A promising therapeutic candidate is sclerostin, a Wnt pathway antagonist and, as such, a negative regulator of bone formation. Sclerostin antibody treatment has demonstrated efficacy and superiority compared to other anabolic treatments for increasing bone formation in both preclinical and clinical settings. Accordingly, it has been suggested that sclerostin antibody treatment is set to achieve market approval by 2017 and aggressively compete as the gold standard for osteoporotic treatment by 2021. In anticipation of phase III trial results which may potentially signify a significant step in achieving market approval here, we review the preclinical and clinical emergence of sclerostin antibody therapies for both osteoporosis and alternative applications. Potential clinical challenges are also explored as well as ongoing developments that may impact on the eventual clinical application of sclerostin antibodies as an effective treatment of osteoporosis.
Studies of effects of isovalent dopants on spectral-kinetic and scintillation characteristics of zinc selenide (2002)

Using the Bridgman technique zinc selenide crystals have been grown spectral-kinetic and scintillation characteristics of zinc selenide crystals containing isovalent dopants have been studied. It is established that on the base of ZnSe crystals, efficient scintillator-Si-PIN-photodiode (S-Si-PIN-PD) detectors of charge particle can be developed.
Highly fluorinated hydrotris(indazolyl)borate calcium complexes: the structure and reactivity heavily depend on the ligand's electronic properties (2014)

Two series of highly fluorinated 3-substituted hydrotris(indazolyl)borate (Fn-Tp4Bo,3R; R = Ph, n = 12; R = CF3, n = 21) complexes of calcium were synthesized by salt metathesis reactions. CaI2 reacts with 2 and 1 equiv. of Tl(F12-Tp4Bo,3Ph) in THF to form the homo- and heteroleptic complexes [Ca(F12-Tp(4Bo,3Ph)*)2] (1) and [(F12-Tp4Bo,3Ph)CaI(THF)] (2), respectively. 1 has C2h symmetry due to a 1,2-borotropic shift. The reaction of CaI2 with 2 equiv. of the more electron poor Tl(F21-Tp4Bo,3CF3) in THF, on the other hand, leads to the salt [Ca(THF)6][F21-Tp4Bo,3CF3]2 (3), with [F21-Tp4Bo,3CF3] acting as a counter-ion. This emphasizes the tuning ability of Fn-Tp4Bo,3R ligands and the consequences on their ability to bind hard electrophilic Ca2+ centers.
Neue Hochschule in Fellbach in Gründung – Grundständig studieren: Es tut sich was (2010)

Nach Bochum, Heidelberg und Bamberg können Abiturienten bald auch in Fellbach bei Stuttgart Physiotherapie ohne vorherige Physiotherapieausbildung studieren. Der Berufsverband VPT wird eigens dafür eine private Hochschule für Gesundheitswissenschaften gründen. Ein weiterer Meilenstein, um den Berufsstand voranzubringen.
Developmental functions of the<i>Distal-less</i>/Dlx homeobox genes (2002)

Distal-less is the earliest known gene specifically expressed in developing insect limbs; its expression is maintained throughout limb development. The homeodomain transcription factor encoded by Distal-less is required for the elaboration of proximodistal pattern elements in Drosophila limbs and can initiate proximodistal axis formation when expressed ectopically. Distal-less homologs, the Dlx genes, are expressed in developing appendages in at least six phyla, including chordates, consistent with requirements for Dlx function in normal appendage development across the animal kingdom. Recent work implicates the Dlx genes of vertebrates in a variety of other developmental processes ranging from neurogenesis to hematopoiesis. We review what is known about the invertebrate and vertebrate Dll/Dlx genes and their varied roles during development. We propose revising the vertebrate nomenclature to reflect phylogenetic relationships among the Dlx genes.
Differential regulation of mouse and human Mu opioid receptor gene depends on the single stranded DNA structure of its promoter and α-complex protein 1 (2017)

The Mu opioid receptor (MOR) mediates various functions of opioid-induced analgesia, euphoria and respiratory depression, and is a major target of opioid analgesics. Understanding of MOR gene expression among species is important for understanding its analgesic function in humans. In the current study, the polypyrimidine/polypurine (PPy/u) region, a key element of MOR gene expression, was compared in humans and mice. The mouse PPy/u element is highly homologous to its human element (84%), and the mouse MOR (mMOR) reporter drives luciferase activity 35-fold more effectively than the human MOR (hMOR) reporter. The structural study of reporter plasmids using S1 nuclease indicates that the mouse PPy/u element has a particular conformational structure, namely a single-stranded DNA (ssDNA) region that promotes strong promoter activity. DNA electrophoretic mobility shift assays demonstrated that the recombinant α-complex protein 1 (α-CP1) is capable of binding to a single-stranded mouse PPy/u sequence. Furthermore, plasmid-expressing α-CP1 activated the expression of a luciferase reporter when cotransfected with a single-stranded (p336/306) construct. In addition, the α-CP1 gene induced the mMOR gene in mouse neuronal cells and did not induce the human neuronal MOR gene. The current study demonstrates that α-CP1 functions as a transcriptional activator in the mMOR gene, but does not function in the hMOR gene due to species-specific structural differences. The differences in human and mouse MOR gene expression are based on α-CP1 and the ssDNA structure of the MOR promoter. The MOR gene is species-specifically regulated, as the PPy/u element adopts a unique species-specific conformation and α-CP1 recruitment.
Myostatin expression is increased by food deprivation in a muscle-specific manner and contributes to muscle atrophy during prolonged food deprivation in mice (2010)

During food deprivation (FD), skeletal muscle protein is broken down to produce amino acids for hepatic gluconeogenesis to maintain blood glucose levels. However, it is unclear what role, if any, the secreted antigrowth factor myostatin (MSTN) plays in the muscle atrophy induced by FD. We therefore examined expression and function of MSTN in FD in mice. Two days of FD significantly decreased muscle mass and protein content and increased mRNA levels of ubiquitin ligases MuRF-1 and atrogin-1 in fast-twitch tibialis anterior (TA) muscle but not slow-twitch soleus (Sol) muscle, while 2 days of refeeding returned these to fed values in TA. MSTN mRNA levels were significantly increased approximately threefold by 2 days, but not 1 day, of FD and returned to fed levels with 2 days of refeeding in TA but were not significantly affected by FD or refeeding in Sol. TA mass decreased to a similar amount after 1 day of FD in wild-type mice and mice null for the MSTN gene but was decreased to a greater amount in wild-type than MSTN-null mice by 2 days of FD. In addition, blood glucose levels decreased and corticosterone levels increased to a greater extent in MSTN-null mice after 2 days of FD, but surprisingly muscle MuRF-1 and atrogin-1 mRNA levels were not affected by the lack of MSTN during FD. Similarly, changes in hepatic enzyme expression in response to FD were identical between wild-type and MSTN-null mice. Our data are consistent with the hypothesis that MSTN is dispensable for the initial atrophy occurring in response to FD but attenuates the decrease in fast-twitch muscle mass during prolonged FD.
Magnetic eye tracking in mice (2017)

Eye movements provide insights about a wide range of brain functions, from sensorimotor integration to cognition; hence, the measurement of eye movements is an important tool in neuroscience research. We describe a method, based on magnetic sensing, for measuring eye movements in head-fixed and freely moving mice. A small magnet was surgically implanted on the eye, and changes in the magnet angle as the eye rotated were detected by a magnetic field sensor. Systematic testing demonstrated high resolution measurements of eye position of <0.1°. Magnetic eye tracking offers several advantages over the well-established eye coil and video-oculography methods. Most notably, it provides the first method for reliable, high-resolution measurement of eye movements in freely moving mice, revealing increased eye movements and altered binocular coordination compared to head-fixed mice. Overall, magnetic eye tracking provides a lightweight, inexpensive, easily implemented, and high-resolution method suitable for a wide range of applications.
Cell type specific applicability of 5‐ethynyl‐2′‐deoxyuridine (EdU) for dynamic proliferation assessment in flow cytometry (2009)

Using the nucleoside analogue EdU (5-ethynyl-2'-deoxyuridine) for thymidine substitution instead of BrdU (5-bromo-2'-deoxyuridine) in cell proliferation assays has recently been proposed. However, the effect of EdU on cell viability, DNA synthesis, and cell cycle progression and consequently its usability for dynamic cell proliferation analysis in vitro has not been explored. We compared the effect of EdU and BrdU incorporation into SK-BR-3 and BT474 breast cancer cells and the impact on cell cycle kinetics, cell viability, and DNA damage. We found that EdU can be used not only for pulse but also for continuous cell labeling and henceforth in high resolution EdU/Hoechst quenching assays. BrdU and EdU proliferation assays based on click chemistry revealed comparable results. However, cell viability of SK-BR-3 breast cancer cells was highly affected by long term exposure to EdU. Both SK-BR-3 as well as BT474 cells show cell cycle arrests upon long term EdU treatment whereas only SK-BR-3 cells were driven into necrotic cell death by long term exposure to EdU. In contrast BT474 cells appeared essentially unharmed by EdU treatment in terms of viability. Consequently using EdU enables highly sensitive and quantitative detection of proliferating cells and facilitates even continuous cell cycle assessment. Nevertheless, potential cellular susceptibility needs to be individually evaluated.
Intersubband plasmons in the quantum limit in gated and aligned carbon nanotubes (2018)

Confined electrons collectively oscillate in response to light, resulting in a plasmon resonance whose frequency is determined by the electron density and the size and shape of the confinement structure. Plasmons in metallic particles typically occur in the classical regime where the characteristic quantum level spacing is negligibly small compared to the plasma frequency. In doped semiconductor quantum wells, quantum plasmon excitations can be observed, where the quantization energy exceeds the plasma frequency. Such intersubband plasmons occur in the mid- and far-infrared ranges and exhibit a variety of dynamic many-body effects. Here, we report the observation of intersubband plasmons in carbon nanotubes, where both the quantization and plasma frequencies are larger than those of typical quantum wells by three orders of magnitude. As a result, we observed a pronounced absorption peak in the near-infrared. Specifically, we observed the near-infrared plasmon peak in gated films of aligned single-wall carbon nanotubes only for probe light polarized perpendicular to the nanotube axis and only when carriers are present either in the conduction or valence band. Both the intensity and frequency of the peak were found to increase with the carrier density, consistent with the plasmonic nature of the resonance. Our observation of gate-controlled quantum plasmons in aligned carbon nanotubes will not only pave the way for the development of carbon-based near-infrared optoelectronic devices but also allow us to study the collective dynamic response of interacting electrons in one dimension.
Orthogonally polarized single sideband modulator (2007)

A modulator design capable of producing a new constant intensity modulation format is proposed. The modulator consists of a Mach-Zehnder interferometer containing two polarization modulators. By shifting the relative phase of the electrical drive signal applied to each polarization modulator by 90 degrees a modulation consisting of a carrier and a single sideband, polarized orthogonal to the carrier, is produced.
AUTOMATIC TARGET MIXING USING LEAST-SQUARES OPTIMIZATION OF GAINS AND EQUALIZATION SETTINGS (2009)

The proposed automatic target mixing algorithm determines the gains and the equalization settings for the mixing of a multi-track recording using a least-squares optimization. These parameters are estimated using a single channel target mix, that is a signal which contains the same audio tracks as the multi-track recording, but that has been previously mixed using some unknown settings. Several tests have been done in order to evaluate the performances of two different approaches to the optimization, namely the sub-band estimator and the FIR filters estimator. The results show that, using the latter technique, the proposed algorithm is able to retrieve the parameters originally applied to the target mix. This achievement can be useful for re-mastering applications, where both the original recording sessions and the final mix are available, but there is the need to retrieve the mixing parameters originally applied to the various audio tracks.
Carrier charge imbalance and optical properties of separate confinement heterostructure quantum well lasers (2000)

Carrier charge imbalance and self-consistent potential effects are systematically studied regarding the optical properties of separate confinement quantum well lasers. Numerical modeling of the typical InP-based laser structure shows that violating the quasineutrality in the vicinity of quantum wells results in a dramatic change in a fraction of carriers trapped in the lasing subbands, and a corresponding modification of laser waveguide properties.
Influence of Lactic Acid Bacteria on Longevity of<i>Caenorhabditis elegans</i>and Host Defense against<i>Salmonella enterica</i>Serovar Enteritidis (2007)

This study aimed to develop a convenient model to investigate the senescence of host defenses and the influence of food and nutrition. A small soil nematode, Caenorhabditis elegans, was grown for 3 days from hatching on a lawn of Escherichia coli OP50 as the normal food source, and subsequently some of the nematodes were fed lactic acid bacteria (LAB). The life spans of worms fed LAB were significantly longer than the life spans of those fed OP50. To investigate the effect of age on host defenses, 3- to 7-day-old worms fed OP50 were transferred onto a lawn of Salmonella enterica serovar Enteritidis for infection. The nematodes died over the course of several days, and the accumulation of salmonella in the intestinal lumen suggested that the worms were infected. The 7-day-old worms showed a higher death rate during the 5 days after infection than nematodes infected at the age of 3 days; no clear difference was observed when the worms were exposed to OP50. We then investigated whether the LAB could exert probiotic effects on the worms' host defenses and improve life span. Seven-day-old nematodes fed LAB from the age of 3 days were more resistant to salmonella than worms fed OP50 until they were infected with salmonella. This study clearly showed that LAB can enhance the host defense of C. elegans and prolong life span. The nematode appears to be an appropriate model for screening useful probiotic strains or dietetic antiaging substances.