gebhart/patrepeval huggingface dataset:
Production of 10,12-dihydroxy-8( E )-octadecenoic acid, an intermediate in the conversion of ricinoleic acid to 7,10,12-trihydroxy-8( E )-octadecenoic acid by Pseudomonas aeruginosa PR3 (2000)

A bacterial isolate, Pseudomonas aeruginosa (PR3), has been reported to produce a new compound, 7,10,12-trihydroxy-8(E)-octadecenoic acid (TOD), from ricinoleic acid (Kuo TM, LK Manthey and CT Hou. 1998. J Am Oil Chem Soc 75: 875–879). The reaction is unique in that it involves an introduction of two additional hydroxyl groups at carbon 7 and 10 and a rearrangement of the double bond from carbon 9–10 (cis) to 8–9 (trans). In an effort to elucidate the metabolic pathway involved in the formation of TOD from ricinoleic acid by PR3, we have isolated another compound from the reaction mixture using HPLC. The structure of the new compound was determined to be 10, 12-dihydroxy-8(E)-octadecenoic acid (DHOD) by GC/MS, FTIR, and NMR. The structural similarity between DHOD and TOD and the results from the time course study of the above two compounds strongly suggested that DHOD was an intermediate in the bioconversion of ricinoleic acid to TOD by PR3. The optimum pH and temperature for the production of DHOD from ricinoleic acid by PR3 was 6.5 and 25°C, respectively. This is the first report on the production of 10,12-dihydroxy-8(E)-octadecenoic acid from ricinoleic acid by PR3. Journal of Industrial Microbiology & Biotechnology (2000) 24, 167–172.
Ureteroscopic Ultrasound Technology to Size Kidney Stone Fragments: Proof of Principle Using a Miniaturized Probe in a Porcine Model (2010)

A 3.6F ultrasound probe can be used to accurately size stone fragments to within 1 mm in a porcine kidney.
A repetitive process approach to modeling and control for ‘bootstrap’ self‐servowriting in hard disk drives (2007)

Abstract This paper considers modeling and correction signal design for self‐servowriting in hard disk drives. Self‐servowriting is a process whereby the position and timing burst information are written on the disk surface by using the servo information from a previously written track (i.e. ‘bootstrap’) as a reference (seed) for writing the burst information on a subsequent track, and repeating the process. An obstacle to this process is radial error propagation, whereby the distortion in the successive written tracks builds up. In this paper, a reference correction signal is designed which when added to the position error signal prevents the radial error propagation. The design of the correction signal is carried out through a repetitive systems analysis and does not require several revolutions of track following in order to compute it. The analysis is verified by means of simulations, which show the containment of radial error propagation. Copyright © 2007 John Wiley & Sons, Ltd.
Supercritical fluid extraction of lanthanides and actinides from solid materials with a fluorinated .beta.-diketone (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSupercritical fluid extraction of lanthanides and actinides from solid materials with a fluorinated .beta.-diketoneYuehe. Lin, R. D. Brauer, K. E. Laintz, and C. M. WaiCite this: Anal. Chem. 1993, 65, 18, 2549–2551Publication Date (Print):September 15, 1993Publication History Published online1 May 2002Published inissue 15 September 1993https://doi.org/10.1021/ac00066a027RIGHTS & PERMISSIONSArticle Views622Altmetric-Citations151LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (465 KB) Get e-Alerts Get e-Alerts
Immunotherapy for Recalcitrant Warts in Children Using Intralesional Mumps or <i>Candida</i> Antigens (2003)

Intralesional injection of mumps and Candida skin test antigens has been shown to be effective in the treatment of warts. Warts are generally difficult to treat in children. To determine the efficacy of intralesional skin test antigen injection for the treatment of resistant warts in children, we treated 47 pediatric patients with one or more warts with intralesional injection of mumps or Candida skin test antigen into one wart. Twenty-two patients (47%) with resistant warts experienced complete resolution of treated warts. An average of 3.78 treatments were necessary. An additional 34% of children had a greater than 25% improvement in their warts. Sixty-eight percent of subjects with more than one wart also noted at least partial resolution (greater than 25% resolution) of untreated warts at distant sites, with 34% experiencing complete resolution. We concluded that intralesional injection of skin test antigens is an effective therapy for children who have recalcitrant, nongenital, cutaneous warts.
Abstract 200: Methylation of GATA4 and GATA5 transcription factor genes in gastric cancers (2010)

Abstract GATA factors comprise a small family of transcription regulatory proteins containing two conserved zinc finger DNA binding domains for the consensus sequence (WGATAR). Among the six GATA factors, GATA-1/2/3 factors are prominently expressed in the hematopoietic cell lineage, while GATA-4/5/6 factors show a partly overlapping expression pattern in the heart and endoderm-derived organs. We previously reported that GATA4 and GATA5 were silenced by their promoter hypermethylation in gastrointestinal, lung and ovarian cancers. However, it remains unknown the mechanism underlying loss of GATA transcription factors in gastric carcinogenesis. To clarify the role of GATA, we examined GATA4/5 methylation and their downstream target genes in gastric cancer (GC). By using methylation specific PCR, the methylation frequency of GATA5 (30, 39%) was significantly higher than that of GATA4 (10, 13%) in 77 GC tissues (P&lt;0.01). However, methylation of GATA6 was not observed in GCs examined. We transiently over-expressed GATA4 or GATA5 in GC cell lines lacking their expression by aberrant methylation. Both GATA4 and GATA5 suppressed colony formation and cell proliferation in MKN74 cells. After GATA4/5 over-expression in MKN74 and KATO-III cells, enhanced expression of numerous genes including ones indicating tumor-suppressive functions, such as TFF1, disabled-2 and IQGAP2, was observed by microarray and RT-PCR analyses. The target genes of GATA4 and GATA5 were closely similar in MKN74 cells. We also preformed siRNA transfection of GATA4 and GATA5 in AGS and HSC43 cells which basally expressed them. Reduced expression of above predicted GATA target genes was detected in these two cell lines after siRNA transfection. These data suggest that loss of GATA4 and GATA5 expression may contribute to gastric carcinogenesis. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 200.
Optimized design method for holographic optical elements (1992)

A method is developed for analytically determining the holographic optical element phase transfer function. The method is based on the minimization of the weighted mean-squared wave-front aberration. It is shown that the wave-front aberration can be described as the linear function for unknown aspheric wave phase correction coefficients. Thus this method does not require many convergence calculations, and the optimal aspheric phase transfer function is obtained analytically. This method is applied to a holographic straight-line scanner design, and the optimal solution is obtained.
WindFloat: A Floating Foundation for Offshore Wind Turbines—Part III: Structural Analysis (2009)

WindFloat is a floating foundation for large offshore wind turbines. This paper describes the structural engineering that was performed as part of the feasibility study conducted for qualification of the technology. Specifically, the preliminary scantling is described and the strength and fatigue analysis methodologies are explained, focusing on the following aspects: • the coupling between the wind turbine and the hull; • the interface between the hydrodynamic loading and the structural response. Prior to reading this manuscript, the reader is strongly encouraged to read the related paper, which focuses on the design basis for the WindFloat, and explores the requirements that must be addressed by the design teams in this new field. An additional paper in this series describes the hydrodynamic analysis and experimental validations.
Building better fibrin knob mimics: an investigation of synthetic fibrin knob peptide structures in solution and their dynamic binding with fibrinogen/fibrin holes (2010)

Fibrin polymerizes via noncovalent and dynamic association of thrombin-exposed "knobs" with complementary "holes." Synthetic knob peptides have received significant interest as a means for understanding fibrin assembly mechanisms and inhibiting fibrin polymerization. Nevertheless, the inability to crystallize short peptides significantly limits our understanding of knob peptide structural features that regulate dynamic knob:hole interactions. In this study, we used molecular simulations to generate the first predicted structure(s) of synthetic knobs in solution before fibrin hole engagement. Combining surface plasmon resonance (SPR), we explored the role of structural and electrostatic properties of knob "A" mimics in regulating knob:hole binding kinetics. SPR results showed that association rates were most profoundly affected by the presence of both additional prolines as well as charged residues in the sixth to seventh positions. Importantly, analyzing the structural dynamics of the peptides through simulation indicated that the 3Arg side chain orientation and peptide backbone stability each contribute significantly to functional binding. These findings provide insights into early fibrin protofibril assembly dynamics as well as establishing essential design parameters for high-affinity knob mimics that more efficiently compete for hole occupancy, parameters realized here through a novel knob mimic displaying a 10-fold higher association rate than current mimics.
Gain measurements of a metal-metal terahertz quantum cascade laser using an integrated terahertz pulse emitter (2011)

A terahertz pulse emitter is fabricated alongside a quantum cascade laser with a metal metal waveguide. Terahertz pulses are used to measure the gain of the laser ridge, which is clamped above threshold to 18 cm−1.
Metal Dusting Resistant Copper-Based Materials (2012)

Cu and Cu-based alloy coatings suppress carbon transfer to the metal surface from carbon-supersaturated gaseous environments. The metal dusting resistance of such coatings has been investigated in CO-H2 gas mixtures at temperatures ranging from 450°C to 700°C. Conventional plating and cladding methods have been used to deposit Cu-based materials on the surfaces of commercial materials such as 2.25Cr-0.5Mo steel and carbon steel. In addition to the performance of Cu-based materials under laboratory conditions, the effect of alloying elements such as Sn, Zn, Ni, Si, and Al in Cu on the corrosion process and the coating/substrate interface stability are discussed with particular attention to commercial scale applications.
Photonically controlled ultrasonic arrays: scenarios and systems (2002)

Wavelength diversity coupled with fiber-based time delay, optical energy delivery, and ultrasonic energy generation and sensing, are proposed for the first time to form a novel, rapidly tunable (e.g., 1 kHz-100 MHz) and wide instantaneous bandwidth (e.g., >50 % bandwidth at center carrier), ultrasonic probe system. This novel photonic system can be used for a wide variety of ultrasonic applications such as therapy, diagnostics, and non-invasive surgery, particularly for intracavity operation with a typical 100 channel/transducer element count. This ultrasonic system architecture relies on a novel multi-wavelength optical delay line module using two high speed (i.e., <10 microseconds wavelength switching time), bulk acousto-optic tunable filters, that provide collinear, high power, multi-wavelength optical input and output beams. This wavelength selection module also provides high optical throughput (e.g., 85% efficiency), and excellent, e.g., <-30 dB optical or <-60 dB electrical blockage of the unwanted leakage (undiffracted) light, preventing noise propagation when such modules are cascaded to provide switched signal time delays.
<i>Escherichia coli</i> DegP Protease Cleaves between Paired Hydrophobic Residues in a Natural Substrate: the PapA Pilin (2002)

The DegP protein, a multifunctional chaperone and protease, is essential for clearance of denatured or aggregated proteins from the inner-membrane and periplasmic space in Escherichia coli. To date, four natural targets for DegP have been described: colicin A lysis protein, pilin subunits and MalS from E. coli, and high-molecular-weight adherence proteins from Haemophilus influenzae. In vitro, DegP has shown weak protease activity with casein and several other nonnative substrates. We report here the identification of the major pilin subunit of the Pap pilus, PapA, as a natural DegP substrate and demonstrate binding and proteolysis of this substrate in vitro. Using overlapping peptide arrays, we identified three regions in PapA that are preferentially cleaved by DegP. A 7-mer peptide was found to be a suitable substrate for cleavage by DegP in vitro. In vitro proteolysis of model peptide substrates revealed that cleavage is dependent upon the presence of paired hydrophobic amino acids; moreover, cleavage was found to occur between the hydrophobic residues. Finally, we demonstrate that the conserved carboxyl-terminal sequence in pilin subunits, although not a cleavage substrate for DegP, activates the protease and we propose that the activating peptide is recognized by DegP's PDZ domains.
SNR improvement of granular perpendicular recording media (2003)

In this paper, we address important issues on the performance improvement of perpendicular magnetic recording (PMR) media, such as noise reduction by adjusting the soft magnetic underlayer (SUL) properties and recording layer and intermediate layer modifications for optimum signal-to-noise (SNR) performance. The permeability of the SUL plays a significant role in writability and SNR. Proper pinning of the SUL should be considered for reducing spike noise. A systematic comparison of alloy and oxide granular media shows that optimized oxide media can provide both better SNR and good thermal stability and is promising for high-density recording.
Efficacy of a respiratory syncytial virus vaccine candidate in a maternal immunization model (2018)

Respiratory syncytial virus (RSV) is the most common cause of bronchiolitis in infants. Maternal immunization is an option to increase maternal antibody levels and protect infants from infection. Here we assess the efficacy of virus-like particle (VLP) vaccine candidates containing stabilized pre-fusion (pre-F) or post-fusion (post-F) conformations of the RSV F protein and the attachment RSV G protein in a maternal immunization model using cotton rats. VLP vaccines containing RSV F and G proteins strongly boost pre-existing RSV immunity in dams preventing their perinatal drop in immunity. Boosting is stronger for the pre-F VLP than for the post-F VLP or purified subunit F protein vaccines, giving an advantage on mothers' protection. VLP immunization of dams provides significant protection to pups from RSV challenge and reduced pulmonary inflammation. Collectively, our results show that a VLP vaccine with RSV F and G proteins is safe and effective for maternal and adult vaccination.
Discovery of the First N-Substituted 4β-Methyl-5-(3-hydroxyphenyl)morphan To Possess Highly Potent and Selective Opioid δ Receptor Antagonist Activity (2003)

A structurally novel opioid delta receptor selective antagonist has been identified. This compound, (+)-5-(3-hydroxyphenyl)-4-methyl-2-(3-phenylpropyl)-2-azabicyclo[3.3.1]non-7-yl-(1-phenyl-1-cyclopentane)carboxamide [(+)-KF4, (+)-4], showed a K(e) value of 0.15 nM in the [(35)S]GTPgammaS functional assay. (+)-KF4 is also a delta inverse agonist with an IC(50) value of 1.8 nM. To our knowledge, this is the first potent and selective delta opioid receptor antagonist from the 5-phenylmorphan class of opioids.
Optical Fourier processing using photoinduced dichroism in a bacteriorhodopsin film (1996)

The photoinduced dichroism in bacteriorhodopsin films has been investigated in view of its application for optical Fourier processing. A simple optical system for Fourier processing is demonstrated that does not require precise alignment and vibration isolation. The basic principle of operation relies on the intensity dependence of photoinduced dichroism in a bacteriorhodopsin film. Experimental results with Fourier processing are shown for edge enhancement.
Respiratory syncytial virus detection in cells and clinical samples by using three new monoclonal antibodies (2013)

Acute respiratory infections caused by the respiratory syncytial virus (RSV) are important health burdens that affect infants worldwide. RSV is also an important cause of morbidity and disease in adults, which causes enormous economic losses. At the present time, RSV infection is diagnosed by immunofluorescence, test pack and/or PCR, obtaining better results with PCR than with any other technique. The production of new monoclonal antibodies (mAbs) capable of detecting RSV in clinical samples is necessary to generate better and faster diagnosis tools for RSV. In this study, three new mAbs, directed against the RSV N and M2-1 proteins, were evaluated for the detection of RSV in clinical samples. Nasopharyngeal swabs were obtained from: 27 RSV-positive patients; 15 human metapneumovirus (hMPV)-positive patients; and 6 healthy controls. To evaluate RSV presence in these samples, clinical samples and RSV-infected cells were tested by Enzyme-Linked ImmunoSorbent Assay (ELISA), flow cytometry, immunofluorescence, and dot-blot assays. Specificity and sensitivity were determined for each mAb by using purified RSV antigens and antigens from different viruses. Infected cells and clinical samples tested with the three new mAbs resulted positive by immunofluorescence, ELISA, flow cytometry, and dot blot. No false positives were obtained in samples infected with other respiratory virus (hMPV) or from healthy controls. These results suggest that these new anti-RSV mAbs can be considered for the rapid and reliable detection of RSV on infected cells and clinical specimens by multiple immunological approaches.
Mapping of the delayed-type hypersensitivity-inducing epitope of secreted protein MPT64 from Mycobacterium tuberculosis (1995)

The gene encoding the immunogenic protein MPT64 found in culture filtrates of Mycobacterium tuberculosis H37Rv was expressed in Escherichia coli K-12 and purified as a recombinant protein. The purified recombinant MPT64 elicited delayed-type hypersensitivity (DTH) in outbred guinea pigs sensitized with Mycobacterium bovis BCG Tokyo. The skin reactions were comparable to those obtained with native MPT64. No skin reactions were observed when either recombinant MPT64 or native MPT64 was used in guinea pigs sensitized with M. bovis BCG Danish 1331. Amino- and carboxy-terminal deletion mutants of MPT64 were purified as fusion proteins for the mapping of DTH-inducing epitopes on recombinant MPT64 by use of the guinea pig skin test model. The part of the molecule responsible for the biological activity was located at the carboxy-terminal end. Further studies with overlapping synthetic peptides have pinpointed the biological activity at a single DTH-inducing epitope consisting of 15 residues between amino acids Gly-173 and Ala-187. Screening by PCR of 56 clinical isolates of M. tuberculosis from Danish and Tanzanian patients demonstrated the presence of mpt64 in all of the strains. These results point to MPT64 as a possible candidate for a skin test reagent specific for diagnosis of human tuberculosis.
Improvement of crystalline quality of epitaxial Si layers by ion-implantation techniques (1979)

We demonstrate that the crystalline quality of Si layers grown on sapphire substrate (SOS) by the CVD method can be greatly improved through the use of implantation of Si ions and subsequent thermal annealing at relatively low temperatures (∼550 °C). This method utilizes an amorphous layer created by ion implantation near the sapphire/Si interface. Subsequent regrowth of this amorphous layer starting from the relatively perfect Si surface region leads to a much improved Si crystalline layer, as evidenced by MeV 4He+ channeling and TEM measurements. When the implantation-formed amorphous layer is located at the outer portion of the Si layer, thermal annealing leads to only a small reduction in the amount of defects in the regrown layer as compared to the unimplanted sample. In these layers, epitaxial regrowth occurs with the same rate and activation energy observed in self-ion-implanted 〈100〉 Si.