gebhart/patrepeval huggingface dataset:
Larval metamorphosis and morphological characteristic analysis of triploid shrimp Fenneropenaeus chinensis (Osbeck, 1765) (2006)

Both MI and MII triploids were successfully produced by heat shock in Chinese shrimp Fenneropenaeus chinensis. The inducing conditions for MI and MII triploids were optimized. The highest inducing rate obtained for MI triploids reached more than 90%, and that for MII triploids reached nearly 100% at the nauplius stage as evaluated using flow cytometry. Comparisons of survival rates at larval stages between triploids and diploids or diploids experiencing treatment and diploids without treatment were performed. At larval stage from nauplii to postlarvae, heat shocks lowered survival at larval stages even if the ploidy was not changed. Ploidy did not affect shrimp larvae survival, and no significant difference was found in the survival of shrimp larvae between MI and MII triploids. Highly significant differences were observed in the morphology of triploids and diploids, and no apparent difference was found in the morphology of MI and MII triploids at the grow-out stages. Discriminating formulae for triploid and diploid shrimp at grow-out stage were developed and could be used to distinguish triploids from diploids based on morphological parameters. MI and MII triploids of shrimp have the potential to be used in aquaculture.
Ferroelectric Space Charge Non-Destructive Read-Out memory (1994)

Abstract The Ferroelectric Space Charge (FESC) Memory is a technique to achieve Non-Destructive Read-Out (NDRO) of a ferroelectric memory that employs standard metal-ferroelectric-metal memory capacitors. The NDRO technique allows the polarization state of the capacitor to be read without altering the polarization. This provides the advantage that the memory capacitor is not subjected to the destructive read/rewrite cycle commonly employed in ferroelectric memories, so the lifetime of the device is limited only by the number of times the memory is written. In addition, the NDRO avoids the period of data volatility in the time interval between the read pulse and the subsequent rewrite of the data. The FESC NDRO approach utilizes changes in the dielectric response of the ferroelectric due to interactions between the internal space charges, the spontaneous polarization, and internal and external electrical potentials. The NDRO is implemented entirely in circuitry and does not require specialized ferroelectric devices. It has been proven in working breadboard circuits and hybrid memory modules. The FESC memory concept is the subject of several patents awarded to Draper Lab.
Chemiluminescence Detector with a Serpentine Flow Cell (2008)

We present a new chemiluminescence detector, with solution channels that have been machined into a Teflon disk and sealed with a sapphire window. The configuration of the flow cell can be conveniently modified by replacing the Teflon disk. A comparison of some existing and novel designs, using the chemiluminescence reaction of morphine with acidic potassium permanganate and the bioluminescence reaction of ATP with the commercially available "BacTiter-Glo" reagent, has revealed that a serpentine channel allows greater quantities of light to be captured than a spiral channel, due to more efficient mixing of the analyte and reagent solutions within the cell.
Traversing probe system. [for gas pressure measurements in gaseous diffusion plants] (1977)

The management of velopharyngeal insufficiency (VPI) in patients with 22q11.2 deletion syndrome (22q11DS) poses a significant clinical challenge due to presence of a large velopharyngeal gap and a relatively high rate of internal carotid artery (ICA) medialization. To our knowledge, we are the first group to have successfully managed VPI in a series of seven pediatric patients with 22q11DS with medialized ICAs via a novel surgical technique involving carotid artery mobilization followed by pharyngeal flap insertion. Thus far, we have found this technique to be reliably safe with no significant morbidity and caregivers have reported postoperative improvement in speech, swallowing and nasal regurgitation symptoms. Herein, we provide a detailed description of our novel surgical approach, including an instructional video, for correction of VPI in patients with medialized ICAs, who have previously had limited management options.
Ergostatrien-3β-ol from<i>Antrodia camphorata</i>Inhibits Diabetes and Hyperlipidemia in High-Fat-Diet Treated Mice via Regulation of Hepatic Related Genes, Glucose Transporter 4, and AMP-Activated Protein Kinase Phosphorylation (2015)

This study was designed to explore the effects and mechanism of ergostatrien-3β-ol (EK100) from the submerged whole broth of Antrodia camphorata on diabetes and dyslipidemia in high fat diet (HFD)-fed mice for 12 weeks. The C57BL/6J mouse fed with a high fat diet (HFD) could induce insulin resistance and hyperlipidemia. After 8 week of induction, mice were receiving EK100 (at three dosages) or fenofibrate (Feno) or rosiglitazone (Rosi) or vehicle by oral gavage 4 weeks afterward. HFD-fed mice display increased blood glucose, glycated hemoglobin (HbA1c), total cholesterol (TC), triglyceride (TG), insulin, and leptin levels. These blood markers were significantly lower in EK100-treated mice, and finally ameliorated insulin resistance. EK100 treatment exhibited reduced hepatic ballooning degeneration and size of visceral adipocytes. Glucose transporter 4 (GLUT4) proteins and phosphorylation of Akt in skeletal muscle were significantly increased in EK100- and Rosi-treated mice. EK100, Feno, and Rosi treatment led to significant increases in phosphorylation of AMP-activated protein kinase (phospho-AMPK) protein in both skeletal muscle and liver. Moreover, EK100 caused a decrease in hepatic expressions of phosphenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6 Pase), and decreased glucose production. EK100 lowered blood TG level by inhibition of hepatic fatty acid synthesis by dampening sterol response element binding protein-1c (SREBP-1c) but increasing expression of peroxisome proliferator activated receptor α (PPARα). Moreover, EK100-treated mice reduced blood TC levels by decreased hepatic expressions of SREBP2, which plays a major role in the regulation of cholesterol synthesis. EK100 increased high-density lipoprotein cholesterol (HDL-C) concentrations by increasing expressions of apolipoprotein A-I (apo A-I) in liver tissue. Our findings manifest that EK100 may have therapeutic potential in treating type 2 diabetes associated with hyperlipidemia in HFD-fed mice by regulation of GLUT4, PEPCK, G6 Pase, SREBP1c, SREBP2, apo A-I, and AMPK phosphorylation.
Discovery and SAR exploration of N-aryl-N-(3-aryl-1,2,4-oxadiazol-5-yl)amines as potential therapeutic agents for prostate cancer (2010)

A new chemical series of antiproliferative compounds was identified via high-throughput screening on DU-145 human prostate carcinoma cell line (hit compound potency - 5.7 microM). Exploration of the two peripheral diversity vectors of the hit molecule in a hit-targeted library and testing of the resulting compounds led to SAR generalizations and identification of the 'best' pharmacophoric moieties. The latter were merged in a single compound that exhibited a 200-fold better potency than the original hit compound. Specific cancer cell cytotoxicity was confirmed for the most potent compounds.
Quantifying abnormal QRS peaks using a novel time-domain peak detection algorithm: Application in patients with cardiomyopathy at risk of sudden death (2015)

Abnormal components in the QRS complex on the surface electrocardiogram have been used to predict sudden cardiac death in patients with heart disease. We propose a novel method to automate detection of abnormal peaks within the QRS complex. The approach involves identification of such peaks from consecutive unfiltered 10-beat QRS averages. A simulation using synthetic QRS peaks is conducted to assess the methods robustness to noise. The performance of the method is tested using high-resolution precordial lead electrocardiograms recorded from normal subjects and patients with cardiomyopathy. The 10-beat average performance is compared to a 100-beat average, as is commonly used in other state-of-the-art QRS component algorithms, and shown to be more sensitive in detecting abnormal QRS peaks. The clinical performance is tested amongst the cardiomyopathy patients and the method is shown to discriminate those at risk of sudden cardiac death with high sensitivity and specificity.
A Self-Referencing Biosensor Based upon a Dual-Mode External Cavity Laser (2013)

We demonstrated a dual-mode external cavity laser biosensor, and developed a self-referencing technique utilizing one of the two lasing modes as reference signal. This system achieves high-Q resonance, high sensitivity label-free detection and eliminates common-mode sources of sensor noise.
Therapeutic Potential of Oridonin and Its Analogs: From Anticancer and Antiinflammation to Neuroprotection (2018)

Oridonin, a diterpenoid natural product commonly used in East Asian herbal medicine, is garnering increased attention in the biomedical community due to its extensive biological activities that include antitumor, anti-inflammatory, antimicrobial, hepatic fibrosis prevention, and neurological effects. Over the past decade, significant progress has been made in structure activity relationship and mechanism of action studies of oridonin for the treatment of cancer and other diseases. This review provides a brief summary on oridonin and its analogs in cancer drug discovery and antiinflammation and highlights its emerging therapeutic potential in neuroprotection applications.
THY1 is a conserved marker of undifferentiated spermatogonia in the pre-pubertal bull testis (2010)

The undifferentiated spermatogonial population consists of stem and progenitor germ cells which function to provide the foundation for spermatogenesis. The stem cell component, termed spermatogonial stem cells (SSCs), is capable of self-renewal and differentiation. These unique attributes have made them a target for novel technologies to enhance reproductive function in males. With bulls, culture and transplantation of SSCs have the potential to enhance efficiency of cattle production and provide a novel avenue to generate transgenic animals. Isolation of SSCs is an essential component for the development of these techniques. In rodents and non-human primates, undifferentiated spermatogonia and SSCs express the surface marker THY1. The hypothesis tested in this study was that THY1 is a conserved marker of the undifferentiated spermatogonial population in bulls. Flow cytometric analyses showed that the THY1+ cell fraction comprises a rare sub-population in testes of pre-pubertal bulls. Immunocytochemical analyses of the isolated THY1+ fraction for expression of VASA showed that this cell population is comprised mostly of germ cells. Additionally, expression of the undifferentiated spermatogonial specific transcription factor promyelocytic leukemia zinc finger (PLZF, ZBTB16) protein was found to be enriched in the isolated THY1+ testis cell fraction. Lastly, xenogeneic transplantation of bull testis cells into seminiferous tubules of immunodeficient mice resulted in greater than sixfold more colonies from isolated THY1+ cells compared to the unselected total testis cell population indicating SSC enrichment. Collectively, these results demonstrate that THY1 is a marker of undifferentiated spermatogonia in testes of pre-pubertal bulls, and isolation of THY1+ cells results in their enrichment from the total testis cell population.
Effect of Keratinocyte Seeding of Collagen-Glycosaminoglycan Membranes on the Regeneration of Skin in a Porcine Model (1998)

A collagen-glycosaminoglycan matrix, impregnated with autologous keratinocytes, was applied as island grafts onto full-thickness porcine wounds to determine whether complete epidermal coverage could be achieved in a single grafting procedure. Twenty-four grafts with seeding densities ranging from 0 to 3,000,000 cells/cm2 were used to determine the kinetics of epidermal coverage. The time sequence of epidermal formation was then studied between days 14 and 28 using four additional grafts, each seeded with a density of 500,000 cells/cm2. Autologous keratinocytes proliferated as the collagen-glycosaminoglycan matrix was vascularized to form a confluent epidermis by 2 weeks in matrices seeded with at least 100,000 cells/cm2. The epidermal thickness and the number of keratinocyte cysts observed in the neodermis at 2 weeks increased linearly with the logarithm of the seeding density. Sequential analysis of neoepidermis showed the nascent epidermis to be hyperplastic, parakeratotic, and focally lacking in granular layer differentiation at 2 weeks. After 2 weeks, it underwent normal maturation and differentiation. Irrespective of seeding density at 2 weeks the collagen-glycosaminoglycan matrix was well vascularized, contained a dense cellular infiltrate, and was almost completely degraded. These studies demonstrate that seeded keratinocytes proliferate and differentiate to form a confluent epidermis by 2 weeks in matrices seeded with at least 100,000 cells/cm2.
Total Ionizing Dose Effects on Long-Term Data Retention Characteristics of Commercial 3-D NAND Memories (2021)

This article evaluates the data retention characteristics of irradiated multilevel-cell (MLC) 3-D NAND flash memories. We irradiated the memory chips by a Co-60 gamma-ray source for up to 50 krad(Si) and then wrote a random data pattern on the irradiated chips to find their retention characteristics. The experimental results show that the data retention property of the irradiated chips is significantly degraded when compared to the un-irradiated ones. We evaluated two independent strategies to improve the data retention characteristics of the irradiated chips. The first method involves high-temperature annealing of the irradiated chips, while the second method suggests preprogramming the memory modules before deploying them into radiation-prone environments.
Synchronization of a picosecond mode-locked dye laser oscillator–amplifier with a streak camera system (1984)

We report a scheme for synchronizing a mode-locked oscillator–amplifier dye laser with a streak camera detection system. A detailed description of the electronics is given. Operational constraints imposed by existing subcomponents of the apparatus are discussed and directions for future refinement outlined.
Abatement of NO/sub x/ in exhaust gases (1988)

A method for pretreating prior to discharge to the atmosphere an industrial exhaust gas contaminated with NO/sub x/, which method comprises: forming at a temperature of at least about 200C a mixture of the exhaust gas and added ammonia, the ammonia being added in an amount to satisfy approximately the stoichiometric requirement for reduction of the NO/sub x/ by NH/sub 3/; and, contacting the mixture at 200C to about 600C and at a gas hourly space velocity effective to substantially reduce the NO/sub x/ content with a commercial, spent organic conversion catalyst, the catalyst comprising a crystalline zeolite having a silica to alumina ratio of at least about 50 and a Constraint Index of 1 to 12.
Time-varying stiffness model of spur gear considering the effect of surface morphology characteristics (2018)

The nonuniform cantilever beam and Hertzian contact model have been widely used to derive the mesh stiffness of spur gear assuming that the contact surface is absolutely frictionless. However, studies have confirmed that machined surfaces are rough in microscale and can be simulated by the Weierstrass–Mandelbort function. In order to get a reasonable and precise mesh stiffness model, the M-B contact model and finite element method are combined to express the local contact stiffness K h . Through the simulation and comparison, the analytical finite element method is proved to be consistent with the traditional models and introduces the roughness parameters of machined tooth surface into the meshing process. Furthermore, the results also show that it is advantageous to improve the total mesh stiffness by increasing the fractal dimension D and input torque T as well as decreasing the roughness parameter G. In this paper, a relationship is built between the total mesh stiffness of gear sets with tooth surface characters and input torque, which can be a guidance in the design of the tooth surface parameters and the choice of the processing method in the future.
Normalization of MicroRNA Quantitative RT-PCR Data in Reduced Scale Experimental Designs (2010)

Proper normalization of quantitative RT-PCR (qRT-PCR) data is a crucial component of gene -expression analysis. Although arbitrarily selected housekeeping genes have been used to normalize many published mRNA RT-PCR datasets, there is a growing awareness that such normalizers should be first validated empirically. The use of stable reference genes is particularly needed for qRT-PCR of microRNA (miRNA), which represent a novel class of biological regulators whose aberrant expression is associated with a range of disorders. Changes in miRNA levels can be modest, and yet have profound cellular consequences. As a result, precise measurements of miRNA expression are critically important. This chapter describes a detailed workflow for the selection of endogenous normalizers using the NormFinder algorithm, -resulting in more accurate miRNA expression profiling results. This approach is particularly well suited to smaller scale miRNA qRT-PCR experimental designs.
Regulation and Role of the Arabidopsis<i>Abscisic Acid-Insensitive 5</i>Gene in Abscisic Acid, Sugar, and Stress Response (2002)

Abscisic acid (ABA) and stress response from late embryonic growth through early seedling development is regulated by a signaling network that includes the Arabidopsis ABA-insensitive (ABI)5 gene, which encodes a basic leucine zipper transcription factor. We have characterized genetic, developmental, and environmental regulation of ABI5 expression. Although expressed most strongly in seeds, the ABI5 promoter is also active in vegetative and floral tissue. Vegetative expression is strongly induced by ABA, and weakly by stress treatments during a limited developmental window up to approximately 2 d post-stratification, but ABA and some stresses can induce expression in specific tissues at later stages. ABI5 expression is autoregulated in transgenic plants and yeast (Saccharomyces cerevisiae), and stress response appears to involve ABI5-dependent and -independent mechanisms. To determine whether ABI5 is necessary and/or sufficient for ABA or stress response, we assayed the effects of increased ABI5 expression on growth and gene expression. Although overexpression of ABI5 confers hypersensitivity to ABA and sugar, as previously described for ABI4 and ABI3 overexpression lines, it has relatively limited effects on enhancing ABA-responsive gene expression. Comparison of expression of eight ABI5-homologous genes shows overlapping regulation by ABI3, ABI4, and ABI5, suggestive of a combinatorial network involving positive and negative regulatory interactions.
Lung IMRT planning with automatic determination of beam angle configurations (2018)

Beam angle configuration is a major planning decision in intensity modulated radiation treatment (IMRT) that has a significant impact on dose distributions and thus quality of treatment, especially in complex planning cases such as those for lung cancer treatment. We propose a novel method to automatically determine beam configurations that incorporates noncoplanar beams. We then present a completely automated IMRT planning algorithm that combines the proposed method with a previously reported OAR DVH prediction model. Finally, we validate this completely automatic planning algorithm using a set of challenging lung IMRT cases. A beam efficiency index map is constructed to guide the selection of beam angles. This index takes into account both the dose contributions from individual beams and the combined effect of multiple beams by introducing a beam-spread term. The effect of the beam-spread term on plan quality was studied systematically and the weight of the term to balance PTV dose conformity against OAR avoidance was determined. For validation, complex lung cases with clinical IMRT plans that required the use of one or more noncoplanar beams were re-planned with the proposed automatic planning algorithm. Important dose metrics for the PTV and OARs in the automatic plans were compared with those of the clinical plans. The results are very encouraging. The PTV dose conformity and homogeneity in the automatic plans improved significantly. And all the dose metrics of the automatic plans, except the lung V<sub>5 Gy</sub>, were statistically better than or comparable with those of the clinical plans. In conclusion, the automatic planning algorithm can incorporate non-coplanar beam configurations in challenging lung cases and can generate plans efficiently with quality closely approximating that of clinical plans.
Magnesium Alloy Development for Automotive Applications (2012)

This paper summarizes the development of new cast and wrought magnesium alloys using computational thermodynamics tools and experimental approach. The Mg-Al-Ca alloys show excellent creep resistance due to the formation of high-temperature (Mg,Al) 2 Ca phase. The Mg-Al-Sn alloys are designed for mechanical properties and corrosion resistance through the optimization of Mg 17 Al 12 and Mg 2 Sn phases in the microstructure. In the Mg-Zn-Ce system, Zn provides strength through solid solution strengthening while Ce increases the ductility via improved texture. Mg-Nd-Zn is a heat-treatable alloy system based on the precipitation hardening of Mg 12 Nd phase.
Cardiorespiratory Sleep Stage Detection Using Conditional Random Fields (2016)

This paper explores the probabilistic properties of sleep stage sequences and transitions to improve the performance of sleep stage detection using cardiorespiratory features. A new classifier, based on conditional random fields, is used in different sleep stage detection tasks (N3, NREM, REM, and wake) in night-time recordings of electrocardiogram and respiratory inductance plethysmography of healthy subjects. Using a dataset of 342 polysomnographic recordings of healthy subjects, among which 135 with regular sleep architecture, it outperforms hidden Markov models and Bayesian linear discriminants in all tasks, achieving an average accuracy of 87.38% and kappa of 0.41 (87.27% and 0.49 for regular subjects) for N3 detection, 78.71% and 0.55 (80.34% and 0.56 for regular subjects) for NREM detection, 88.49% and 0.51 (87.35% and 0.57 for regular subjects) for REM, and 85.69% and 0.51 (90.42% and 0.52 for regular subjects) for wake. In comparison with the state of the art, and having been tested on a much larger dataset, the classifier was found to outperform most of the work reported in the literature for some of the tasks, in particular for subjects with regular sleep architecture. It achieves a comparable accuracy for N3, higher accuracy and kappa for REM, and higher accuracy and comparable kappa for NREM than the best performing classifiers described in the literature.
Computer-controllable wedged-plate optical variable attenuator (1980)

A computer-controllable variable optical attenuator has been developed and tested that has a wide dynamic range, wide spectral range, and is suitable for applications with high peak and average power laser sources. The device is based on Fresnel transmission through two pairs of wedged plates. A 35-dB dynamic range, an insertion loss of 1%, a precision of better than 1% and beam offset and deflection of <0.5 mm and 0.5 mrad, respectively, are demonstrated.