gebhart/patrepeval huggingface dataset:
Abstract 16349: A Novel Class of Inhibitors That Are Specific to Human NPP1 Prevent Pathologic Mineralization of Valve Interstitial Cells (2014)

BACKGROUND: Ectonucleotide pyrophosphatase/phosphodiesterase 1 (NPP1) is an ectoenzyme, which plays a role into several disorders including calcific aortic valve disease (CAVD). So far, compounds that have been developed as inhibitors of NPP1 lack of potency and specificity. Quinazolin-4-piperidin-4-methyl sulfamide (QPS) derivatives have been described as potent inhibitors of NPP1. However, their mode of inhibition as well as their selectivity and capacity to modify biological processes have not been investigated. METHOD: We have investigated the potency and selectivity of QPS derivatives in inhibiting NPP1 by enzymatic activities. The biological effect of QPS derivatives was documented on the mineralization of valve interstitial cell cultures, apoptosis assay, as well as quantitative polymerase chain reaction. RESULTS: We documented that QPS1 derivative is a potent (67.9 ±5.3 nM) and selective non-competitive inhibitor of human NPP1. Moreover, QPS1 also significantly inhibited the K121Q NPP1 gene variant (ki 51.9±9.8 nM), which is prevalent in the general population. QPS1 did not significantly alter the activity of other nucleotide metabolising enzymes expressed at the cell surface, namely NPP3, NTPDases (1-3), ecto-5’-nucleotidase and ALP. Importantly, QPS1 in the low micromolar range (≤10μM) prevented phosphate-induced mineralization of VICs and lowered the rise of osteogenic genes as expected for NPP1 inhibition. CONCLUSION: We provide evidence that QPS1 is a potent and selective non-competitive inhibitor of NPP1 that prevents pathologic mineralization in a cellular model.
Enzymatic production of ribavirin from purine nucleosides by Brevibacterium acetylicum ATCC 954. (1988)

Ribavirin (l-β-D-ribofuranosyl-l, 2, 4-triazole-3-carboxamide; Virazole®), which is known as a potent antiviral agent, was synthesized directly from purine nucleosides and l, 2, 4-triazole-3-carboxamide (TCA) by a newly selected bacterium, Brevibacterium acetylicum ATCC 954. Among the purine nucleosides tested, guanosine and xanthosine were selected as the best substrates as to ribavirin production. The highest amount of ribavirin produced was 229 mM from 300 mM guanosine and 300 mM TCA on 96 hr reaction at 60°C. In this system involving ATCC 954, ribavirin was effectively even produced at a limited concentration of phosphate (about 10mM). ATCC 954 could also produce ribavirin directly from inosine and TCA in a high yield, but could hardly produce it directly from adenosine and TCA. In contrast, a bacterium belonging to the genus, Bacillus, could produce ribarivin directly from adenosine and TCA in a high yield.
[DLys<sup>6</sup>]‐luteinizing hormone releasing hormone–curcumin conjugate inhibits pancreatic cancer cell growth <i>in vitro</i> and <i>in vivo</i> (2011)

Pancreatic ductal adenocarcinomas are invariably lethal, and developing effective treatments that have minimal side effects is a challenge. Previous studies from our laboratory have shown that conjugates of cell membrane disrupting lytic peptides and luteinizing hormone releasing hormone (LHRH) target and destroy human prostate and breast cancer cells in xenografts in the nude mouse model (Hansel et al., Mol Cell Endocrinol 2007;260-262:183-9; Hansel et al., Mol Cell Endocrinol 2007;269:26-33), which express LHRH receptors. The objectives of our study were to synthesize a bioconjugate of LHRH analog ([DLys(6)]-LHRH) and a dietary microchemical (curcumin) and test the hypothesis that [DLys(6)]-LHRH-curcumin targets and inhibits pancreatic cancer cell growth in vitro and in vivo. In in vitro studies, we determined by confocal microscopy, flow cytometry analysis and reverse transcriptase-polymerase chain reaction that MIAPaCa-2, Panc-1 and BxPC-3 pancreatic cancer cell lines express LHRH receptors. [DLys(6)]-LHRH-curcumin inhibited cell proliferation of pancreatic cancer cell lines and induced apoptotic cell death (p < 0.05). Apoptosis was induced by cleavage of polyadenosine-5'-diphosphate-ribose-polymerase and caspase-3. The activity of [DLys(6)]-LHRH-curcumin was equal to free curcumin at equimolar concentrations in vitro. Unlike curcumin itself, the [DLys(6)]-LHRH-curcumin conjugate is water soluble which allows its intravenous administration. In two in vivo studies, [DLys(6)]-LHRH-curcumin given intravenously caused a significant (p < 0.01) reduction in tumor weights and volumes, and free curcumin given by gavage at an equal dose failed to cause a significant reduction in tumor weights and volumes in the nude mouse pancreatic cancer model. [DLys(6)]-LHRH-curcumin treatment enhanced apoptosis compared to [DLys(6)]-LHRH and vehicle-treated controls in tumor tissue. In conclusion, [DLys(6)]-LHRH-curcumin may be useful in treating pancreatic cancer.
Structured Low-Density Parity-Check Code Design for Next Generation Digital Video Broadcast (2006)

We describe a novel design for low-density parity-check (LDPC) codes that eliminates the routing problem associated with LDPC decoder implementation resulting in a small silicon area in application specific integrated circuits (ASICs). Unlike previous solutions that require a large number of small and hence area inefficient memory blocks, this design allows collision-free memory access on a single memory block by imposing certain restrictions on the parity check matrix of the codes. Therefore, it leads to substantially smaller circuits. Furthermore the resulting codes have outstanding error rate performance very close to Shannon limit for a wide range of throughputs from 0.5 bits/symbol up to 4 bits/symbol. As a result LDPC codes designed with this method have been standardized for the next generation digital video broadcasting (DVB).
<title>Method for embedding optical fibers and optical fiber sensors in metal parts and structures</title> (1991)

A technique for embedding one or more optical fibers in a cast metal part or structure while maintaining optical transmission through the fiber is presented. This technique provides nondestructive monitor of internal perturbations of the structure. Application of the method to embedded fiber optic sensors in metallic structures and to fiber-embedded metal feedthrough are reported and the performances of temperature and ultrasound fiber sensor embedded in a cast aluminum block are demonstrated.
Capture immunoassay for the diagnosis of bovine mastitis using a monoclonal antibody to polymorphonuclear granulocytes (1992)

A direct capture enzyme-linked immunosorbent assay (ELISA) was developed to measure elevated polymorphonuclear granulocyte (PMN) antigens using horseradish peroxidase (EC 1.11.1.7) conjugated rabbit polyclonal anti-PMN antisera and a monoclonal antibody specific for PMN cells. Optical densities obtained in the ELISA were used to predict the cell counts of milk samples. Predicted counts were not significantly different from actual somatic cell counts (SCC). In a total of 156 bovine milk samples the correlation coefficient between somatic cell counting, taking greater than 500,000 cells/ml as being indicative of mastitis, and the assay was 0.94, yielding an assay sensitivity of 95.2% and a specificity of 97.3%. In further trials the ELISA could detect elevated PMN antigens in milk with SCC as low as 100,000 cells/ml. The results indicate that the monoclonal antibody based direct ELISA has excellent potential in the detection and determination of bovine mastitis.
Macular Pigment Lutein Is Antiinflammatory in Preventing Choroidal Neovascularization (2007)

Lutein treatment led to significant suppression of CNV development together with inflammatory processes including NF-kappaB activation and subsequent upregulation of inflammatory molecules, providing molecular evidence of potential validity of lutein supplementation as a therapeutic strategy to suppress CNV.
Capacity, delay and mobility in wireless ad-hoc networks (2003)

Network throughput and packet delay are two important parameters in the design and the evaluation of routing protocols for ad-hoc networks. While mobility has been shown to increase the capacity of a network, it is not clear whether the delay can be kept low without trading off the throughput. We consider a theoretical framework and propose a routing algorithm which exploits the patterns in the mobility of nodes to provide guarantees on the delay. Moreover, the throughput achieved by the algorithm is only a poly-logarithmic factor off from the optimal. The algorithm itself is fairly simple. In order to analyze its feasibility and the performance guarantee, we used various techniques of probabilistic analysis of algorithms. The approach taken in this paper could be applied to the analyses of some other routing algorithms for mobile ad hoc networks proposed in the literature.
Blocking Entry of Hepatitis B and D Viruses to Hepatocytes as a Novel Immunotherapy for Treating Chronic Infections (2020)

We here describe a novel immunotherapy for chronic HBV/HDV that targets viral entry to complement NAs and coming therapies inhibiting viral maturation.
Perovskite superlattices with efficient carrier dynamics (2021)

Abstract Compared with their three-dimensional counterparts, low-dimensional metal halide perovskites with periodic inorganic/organic structures have shown promising stability and hysteresis-free electrical performance, which paves the way for next-generation optoelectronic devices. However, when integrated in devices, they have relatively limited efficiencies because devices usually require carrier transport through the film thickness direction. In conventionally grown single crystals, the carrier transport in the thickness direction is hindered by the insulating organic spacers. In addition, the strong quantum confinement from the organic spacers limits the generation and transport of free carriers. The carrier dynamics is further compromised by the presence of grain boundaries in polycrystals. Here, we report a low-dimensional metal halide perovskite superlattice with efficient carrier transport in three dimensions by epitaxial growth. Epitaxy on a slightly lattice-mismatched substrate compresses the organic spacers in the superlattice, which weakens the quantum confinement and further improves carrier dynamics. The performance of a low-dimensional perovskite superlattice solar cell has been certified under the quasi-steady state for the first time. Moreover, the device shows an unusually high open-circuit voltage, due to a unique intra-band exciton relaxation mechanism.
New fatty monoesters of erythromycin A. (1990)

New fatty polyenic (linoleic, linolenic, arachidonic, linoelaidic) mono esters of erythromycin A have been synthesized by using various reagents such as acyl chloride, carboxylic acid anhydride, and mixed carbonic anhydride. These different ways of activating the fatty acid allowed a regioselectivity of esterification at position 2' of the desosamine ring or position 4" of the cladinose ring of erythromycin A. The in vitro antibacterial properties of these new esters against members of the resident flora of the human skin were determined and compared with those of erythromycin A. The number and the stereochemistry of the double bonds seem to play a crucial role in the expression of the in vitro antibacterial activity.
Monoclonal Antibodies Recognizing the Non-Tandem Repeat Regions of the Human Mucin MUC4 in Pancreatic Cancer (2011)

The MUC4 mucin is a high molecular weight, membrane-bound, and highly glycosylated protein. It is a multi-domain protein that is putatively cleaved into a large mucin-like subunit (MUC4α) and a C-terminal growth-factor like subunit (MUC4β). MUC4 plays critical roles in physiological and pathological conditions and is aberrantly overexpressed in several cancers, including those of the pancreas, cervix, breast and lung. It is also a potential biomarker for the diagnosis, prognosis and progression of several malignancies. Further, MUC4 plays diverse functional roles in cancer initiation and progression as evident from its involvement in oncogenic transformation, proliferation, inhibition of apoptosis, motility and invasion, and resistance to chemotherapy in human cancer cells. We have previously generated a monoclonal antibody 8G7, which is directed against the TR region of MUC4, and has been extensively used to study the expression of MUC4 in several malignancies. Here, we describe the generation of anti-MUC4 antibodies directed against the non-TR regions of MUC4. Recombinant glutathione-S-transferase (GST)-fused MUC4α fragments, both upstream (MUC4α-N-Ter) and downstream (MUC4α-C-Ter) of the TR domain, were used as immunogens to immunize BALB/c mice. Following cell fusion, hybridomas were screened using the aforementioned recombinant proteins ad lysates from human pancreatic cell lines. Three anti MUC4α-N-Ter and one anti-MUC4α-C-Ter antibodies were characterized by several inmmunoassays including enzyme-linked immunosorbent assay (ELISA), immunoblotting, immunofluorescene, flow cytometry and immunoprecipitation using MUC4 expressing human pancreatic cancer cell lines. The antibodies also reacted with the MUC4 in human pancreatic tumor sections in immunohistochemical analysis. The new domain-specific anti-MUC4 antibodies will serve as important reagents to study the structure-function relationship of MUC4 domains and for the development of MUC4-based diagnostics and therapeutics.
Nanomechanical Torsional Resonators for Frequency-Shift Infrared Thermal Sensing (2013)

We investigate use of nanomechanical torsional resonators for frequency-shift-based infrared (IR) thermal sensing. Nanoscale torsion rods, ~1 μm long and 50-100 nm in diameter, provide both extraordinary thermal isolation and excellent angular displacement and torque sensitivities, of order ~10(-7) rad·Hz(-1/2) and ~10(-22) (N·m) Hz(-1/2), respectively. Furthermore, these nanorods act as linear torsional springs, yielding a maximum angular displacement of 3.6° and a dynamic range of over 100 dB; this exceeds the performance of flexural modes by as much as 5 orders of magnitude. These attributes lead to superior noise performance for torsional-mode sensing. We demonstrate the operational principles of torsional-mode IR detection, attaining an uncooled noise equivalent temperature difference (NETD) of 390 mK. By modeling the fundamental noise processes, we project that further reduction of device size can significantly improve thermal responsivity; a room-temperature NETD below 10 mK appears feasible.
Hybrid multi-objective motion planning of Parallel Kinematic Machines (2010)

In this paper we consider the problem of multi-objective trajectory planning to Parallel Kinematic Machines (PKMs). A two stage system is developed. In a first stage is an offline planning based on robot kinematics and dynamics, including actuators, is performed to generate a large dataset of trajectories, these trajectory cover mostly of the robot workspace and minimize time and energy, while avoiding singularities and limits on joint angles, rates, accelerations and torques. An augmented Lagrangian decoupling to solve the resulting non-linear constrained optimal control problem. The offline-planning outcomes are then used to build a data-driven neuro-fuzzy inference system to learn and capture the desired dynamic behavior of the PKM. Once this system is trained, it is used to achieve near-optimal online planning with a reasonable time complexity. Simulations proving the effectiveness of this approach on a 2-degrees of freedom planar PKM are given and discussed.
Efficient pnp characteristics of pMOS transistors in sub-0.13µm ESD protection circuits (2002)

An analysis of pMOS transistors in snapback conduction mode for a sub-0.13µm technology will be presented. The pMOS n-well confined behavior vs. nMOS will be discussed. It will be shown that n-well resistance control under ESD conditions is the key factor to maintaining a sufficient ballast resistance in fully silicided technologies. The snapback and breakdown behavior will be discussed with respect to both physical mechanisms and process features. New reliability issues arising from a highly performing pMOS pull up transistor will be described. Possible solutions at the process, device and circuit level will be presented to minimize these reliability concerns.
Estimating the Breast Surface Using UWB Microwave Monostatic Backscatter Measurements (2007)

This paper presents an algorithm for estimating the location of the breast surface from scattered ultrawideband (UWB) microwave signals recorded across an antenna array. Knowing the location of the breast surface can improve imaging performance if incorporated as a priori information into recently proposed microwave imaging algorithms. These techniques transmit low-power microwaves into the breast using an antenna array, which in turn measures the scattered microwave signals for the purpose of detecting anomalies or changes in the dielectric properties of breast tissue. Our proposed surface identification algorithm consists of three procedures, the first of which estimates M points on the breast surface given M channels of measured microwave backscatter data. The second procedure applies interpolation and extrapolation to these M points to generate N > M points that are approximately uniformly distributed over the breast surface, while the third procedure uses these N points to generate a 3-D estimated breast surface. Numerical as well as experimental tests indicate that the maximum absolute error in the estimated surface generated by the algorithm is on the order of several millimeters. An error analysis conducted for a basic microwave radar imaging algorithm (least-squares narrowband beamforming) indicates that this level of error is acceptable. A key advantage of the algorithm is that it uses the same measured signals that are used for UWB microwave imaging, thereby minimizing patient scan time and avoiding the need for additional hardware.
Lead‐Free Piezoceramics. (2004)

Abstract For Abstract see ChemInform Abstract in Full Text.
Controlled/“Living” Radical Polymerization Applied to Water-Borne Systems (2000)

Atom transfer radical polymerization (ATRP), a controlled/“living” radical polymerization, has been extended to water-borne polymerization systems. In order to obtain a controlled ATRP reaction in a water-borne system, various criteria must be met, which are not necessary when conducted in organic solvents. The effect of surfactant, monomer, catalyst and initiator employed will be discussed, as each had a profound effect on the success of the ATRP reaction.
A Novel Tilt and Acceleration Measurement System Based on Hall-Effect Sensors Using Neural Networks (2022)

A tilt sensor is a device used to measure the tilt on many axes of a reference point. Tilt sensors measure the bending position according to gravity and are used in many applications. Slope sensors allow easy detection of direction or slope in the air. These tilt gauges have become increasingly popular and are being adapted for a growing number of high-end applications. As an example of practical application, the tilt sensor provides valuable information about an aircraft’s vertical and horizontal tilt. This information also helps the pilot understand how to deal with obstacles during flight. In this paper, Hall-effect effective inclination and acceleration sensor design, which makes a real-time measurement, have been realized. 6 Hall-effect sensors with analog output (UGN-3503) have been used in the sensor structure. These sensors are placed in a machine, and the hall sensor outputs are continuously read according to the movement speed and direction of the sphere magnet placed in the assembly. Hall sensor outputs produce 0–5 Volt analog voltage according to the position of the magnet sphere to the sensor. It is clear that the sphere magnet moves according to the inclination of the mechanism when the mechanism is moved angularly, and the speed of movement from one point to the other changes according to the movement speed. Here, the sphere magnet moves between the hall sensors in the setup according to the ambient inclination and motion acceleration. Each sensor produces analog output values in the range of 0–5 V instantaneous according to the position of the spheroid. Generally defined, according to the sphere magnet position and movement speed, the data received from the hall sensors by the microcontroller have been sent to the computer or microcomputer unit as UART. In the next stage, the actual sensor has been removed. The angle and acceleration values have been continuously produced according to the mechanism’s movement and output as UART. Thanks to the fact that the magnet is not left idle and is fixed with springs, problems such as vibration noises and wrong movements and the magnet leaning to the very edge and being out of position even at a slight inclination are prevented. In addition, the Hall-effect sensor outputs are given to an artificial neural network (ANN), and the slope and acceleration information is estimated in the ANN by training with the data obtained from the real-time slope and accelerometer sensor.
Effect of Bottom Boundary on VIV for Energy Harnessing at 8×103<Re<1.5×105 (2009)

The concept of extracting energy from ocean/river currents using vortex induced vibration was introduced at the OMAE2006 Conference. The vortex induced vibration aquatic clean energy (VIVACE) converter, implementing this concept, was designed and model tested; VIV amplitudes of two diameters were achieved for Reynolds numbers around 105 even for currents as slow as 1.6 kn. To harness energy using VIV, high damping was added. VIV amplitude of 1.3 diameters was maintained while extracting energy at a rate of PVIVACE=0.22×0.5×pU3DL at 1.6 kn. Strong dependence of VIV on Reynolds number was proven for the first time due to the range of Reynolds numbers achieved at the Low-Turbulence Free Surface Water (LTFSW) Channel of the University of Michigan. In this paper, proximity of VIVACE cylinders in VIV to a bottom boundary is studied in consideration of its impact on VIV, potential loss of harnessable energy, and effect on soft sediments. VIV tests are performed in the LTFSW Channel spanning the following ranges of parameters: Re∊[8×103–1.5×105], m∗∊[1.0–3.14], U∊[0.35–1.15 m/s], L/D∊[6–36], closest distance to bottom boundary (G/D)∊[4−0.1], and m∗ζ∊[0.14–0.26]. Test results show strong impact for gap to diameter ratio of G/D<3 on VIV, amplitude of VIV, range of synchronization, onset of synchronization, frequency of oscillation, hysteresis at the onset of synchronization, and hysteresis at the end of synchronization.
Inverted repeat sequences flank a Bacillus thuringiensis crystal protein gene (1984)

Two sets of inverted repeat DNA sequences, IR2150 and IR1750, were discovered flanking the crystal protein gene on the 75-kilobase plasmid of Bacillus thuringiensis subsp. kurstaki HD73. A restriction map of ca. 40 kilobases around the crystal protein gene was constructed, and the positions of the copies of IR2150 and IR1750 were determined. Three copies of IR2150 were found flanking the crystal protein gene in an inverted orientation, and one partial and three intact copies of IR1750 were found in both inverted and direct orientations around the gene. Hybridization experiments with fragments from within IR2150 and IR1750 demonstrated the presence of multiple copies of these sequences on the chromosome of B. thuringiensis subsp. kurstaki HD73 and also revealed a strong correlation between the presence of these sequences and the presence of the crystal protein gene on plasmids from 14 strains of B. thuringiensis.