gebhart/patrepeval huggingface dataset:
PmVRP15, a Novel Viral Responsive Protein from the Black Tiger Shrimp, Penaeus monodon, Promoted White Spot Syndrome Virus Replication (2014)

Suppression subtractive hybridization of Penaeus monodon hemocytes challenged with white spot syndrome virus (WSSV) has identified the viral responsive gene, PmVRP15, as the highest up-regulated gene ever reported in shrimps. Expression analysis by quantitative real time RT-PCR revealed 9410-fold up-regulated level at 48 h post WSSV injection. Tissue distribution analysis showed that PmVRP15 transcript was mainly expressed in the hemocytes of shrimp. The full-length cDNA of PmVRP15 transcript was obtained and showed no significant similarity to any known gene in the GenBank database. The predicted open reading frame of PmVRP15 encodes for a deduced 137 amino acid protein containing a putative transmembrane helix. Immunofluorescent localization of the PmVRP15 protein revealed it accumulated around the nuclear membrane in all three types of shrimp hemocytes and that the protein was highly up-regulated in WSSV-infected shrimps. Double-stranded RNA interference-mediated gene silencing of PmVRP15 in P. monodon significantly decreased WSSV propagation compared to the control shrimps (injected with GFP dsRNA). The significant decrease in cumulative mortality rate of WSSV-infected shrimp following PmVRP15 knockdown was observed. These results suggest that PmVRP15 is likely to be a nuclear membrane protein and that it acts as a part of WSSV propagation pathway.
Case Tool for Developing Process Control Specifications in Dairy Manufacturing (1994)

Abstract: A computer aided software engineering (CASE) tool designed to assist the task of developing the specification of control software for programmable logic controllers for dairy process manufacturing is described. The CASE tool, called the specification assistant, is a part of a larger development environment that may ultimately include automatic generation of PLC code from the specification. The specification assistant includes a set of rule bases and associated rule-base editors that permit customization of the tool to particular manufacturing sites. It aims to standardize control code specifications, support reuse of specifications, allow inspection of specifications through simulation and reports, and facilitate the long term maintenance of specifications. The specification assistant was developed to support the domain of dairy process manufacturing in New Zealand. A domain analysis was performed to identify a specification language
The solid-state architecture of a metallosupramolecular polyelectrolyte (2006)

Self-assembly of Fe(II) and the ditopic ligand 1,4-bis(2,2':6',2''-terpyridine-4'-yl)benzene results in equilibrium structures in solutions, so-called metallosupramolecular coordination polyelectrolytes (MEPEs). It is exceedingly difficult to characterize such macromolecular assemblies, because of the dynamic nature. Therefore, hardly any structural information is available for this type of material. Here, we show that from dilute solutions, where small aggregates predominate, it is possible to grow nanoscopic crystals at an interface. A near atomic resolution structure of MEPE is obtained by investigating the nanoscopic crystals with electron diffraction in combination with molecular modeling. The analysis reveals a primitive monoclinic unit cell (P2(1)/c space group, a = 10.4 A, b = 10.7 A, c = 34.0 A, alpha = gamma = 90 degrees , beta = 95 degrees , rho = 1.26 g/cm(3), and Z = 4). The MEPE forms linear rods, which are organized into sheets. Four sheets intersect the unit cell, while adjacent sheets are rotated by 90 degrees with respect to each other. The pseudooctahedral coordination geometry of the Fe(II) centers is confirmed by Mössbauer spectroscopy. The combination of diffraction and molecular modeling presented here may be of general utility to address problems in structural materials science.
Short wavelength x-ray laser research at the Lawrence Livermore National Laboratory (1992)

Laboratory x-ray lasers are currently being studied by researchers worldwide. This paper reviews some of the recent work carried out at Lawrence Livermore National Laboratory. Laser action has been demonstrated at wavelengths as short as 35.6 Å while saturation of the small signal gain has been observed with longer wavelength schemes. Some of the most successful schemes to date have been collisionally pumped x-ray lasers that use the thermal electron distribution within a laser-produced plasma to excite electrons from closed shells in neon- and nickel-like ions to metastable levels in the next shell. Attempts to quantify and improve the longitudinal and transverse coherence of collisionally pumped x-ray lasers are motivated by the desire to produce sources for specific applications. Toward this goal there is a large effort underway to enhance the power output of the Ni-like Ta x-ray laser at 44.83 Å as a source for x-ray imaging of live cells. Improving the efficiency of x-ray lasers in order to produce saturated output with smaller pump lasers is also a goal of this work.
Bimetallic Au–Ni Nanoparticles Embedded in SiO<sub>2</sub> Nanospheres: Synergetic Catalysis in Hydrolytic Dehydrogenation of Ammonia Borane (2010)

Gold-nickel nanoparticles (NPs) of 3-4 nm diameter embedded in silica nanospheres of around 15 nm have been prepared by using [Au(en)(2)Cl(3)] and [Ni(NH(3))(6)Cl(2)] as precursors in a NP-5/cyclohexane reversed-micelle system, and by in situ reduction in an aqueous solution of NaBH(4)/NH(3)BH(3). Compared with monometallic Au@SiO(2) and Ni@SiO(2), the as-synthesized Au-Ni@SiO(2) catalyst shows higher catalytic activity and better durability in the hydrolysis of ammonia borane, generating a nearly stoichiometric amount of hydrogen. During the generation of H(2), the synergy effect between gold and nickel is apparent: The nickel species stabilizes the gold NPs and the existence of gold helps to improve the catalytic activity and durability of the nickel NPs.
Trace elemental composition of curry by inductively coupled plasma optical emission spectrometry (ICP-OES) (2008)

A methodology based on inductively coupled plasma optical emission spectrometry (ICP-OES) after microwave-assisted acid digestion was developed to determine the content of traces elements in curry samples from the Spanish market. The methodology was validated in terms of accuracy by the analysis of citrus and tomato leaf reference materials achieving comparable results with the certified values. The trace metal content of curry samples was compared with data available from previously published reports concerning Indian samples, especially in terms of heavy metal composition, in order to guarantee the quality of the commercially available spices in the European countries. Values found for the analysis of arsenic, lead and cadmium were significantly lower than the maximum limit allowed by European Union statutory limits for heavy metals and lower than those obtained for Indian curry leaves reported by Indian research teams by using neutron activation and γ-ray analysis.
Numerical study on bearing behavior of pile considering sand particle crushing (2013)

The bearing mechanism of pile during installation and loading process which controls the deformation and distribution of strain and stress in the soil surrounding pile tip is complex and full of much uncertainty. It is pointed out that particle crushing occurs in significant stress concentrated region such as the area surrounding pile tip. The solution to this problem requires the understanding and modeling of the mechanical behavior of granular soil under high pressures. This study aims to investigate the sand behavior around pile tip considering the characteristics of sand crushing. The numerical analysis of model pile loading test under different surcharge pressure with constitutive model for sand crushing is presented. This constitutive model is capable of predicting the dilatancy of soil from negative to positive under low confining pressure and only negative dilatancy under high confining pressure. The predicted relationships between the normalized bearing stress and normalized displacement are agreeable with the experimental results during the entire loading process. It is estimated from numerical results that the vertical stress beneath pile tip is up to 20 MPa which is large enough to cause sand to be crushed. The predicted distribution area of volumetric strain represents that the distributed area shaped wedge for volumetric contraction is beneath pile tip and distributed area for volumetric expansion is near the pile shaft. It is demonstrated that the finite element formulation incorporating a constitutive model for sand with crushing is capable of producing reasonable results for the pile loading problem.
Design and Implementation of Automatic Attendance Check System Using BLE Beacon (2015)

As the use of smart devices is being activated in the education sector the introduction of smart education using smart devices is coming into full swing. For universities in 2012 the Ministry of Education, Science and Technology is establishing a smart campus establishment plan and promoting it so that students can use electronic devices including smart devices to learn anywhere and anytime. For it is the study designed and implemented a convenient and practical attendance management system. The suggested system automatically uses the Bluetooth 4.0 communication of the students’ smart phones when the student enters the lecture hall of the course to check the location of the Beacon and automatically acknowledges attendance if it is valid location. It is characterized by the fact that there is no action to be taken on the part of the student or professor for checking attendance. Also the system recognizes the student and professor modes using the smart phones’ number and registers students and professors. Thus it can be used more conveniently because there are no other actions required.
Reactive sucrose derivatives (1990)

De nouveaux esters et ethers du sucrose avec des groupes fonctionnels presentant des groupes hydroxiles de reactivites differentes sont etudies: les esters methacryliques de sucrose; les gels hydrophiles de methacrylate de sucrose pour l'immobilisation des reactifs; les derives du sucrose avec des groupes amides de l'acide carbonique ou des groupes amides N-methyloles; des derives du sucrose avec des groupes photoactifs pour la preparation de composes photoresistants; des derives du sucrose avec des groupes primaires amines et les amides derivees de leurs acides gras; des derives du sucrose hydrosolubles avec des groupes biologiquement actifs
Recognizing Human-Object Interactions in Still Images by Modeling the Mutual Context of Objects and Human Poses (2012)

Detecting objects in cluttered scenes and estimating articulated human body parts from 2D images are two challenging problems in computer vision. The difficulty is particularly pronounced in activities involving human-object interactions (e.g., playing tennis), where the relevant objects tend to be small or only partially visible and the human body parts are often self-occluded. We observe, however, that objects and human poses can serve as mutual context to each other-recognizing one facilitates the recognition of the other. In this paper, we propose a mutual context model to jointly model objects and human poses in human-object interaction activities. In our approach, object detection provides a strong prior for better human pose estimation, while human pose estimation improves the accuracy of detecting the objects that interact with the human. On a six-class sports data set and a 24-class people interacting with musical instruments data set, we show that our mutual context model outperforms state of the art in detecting very difficult objects and estimating human poses, as well as classifying human-object interaction activities.
High-power narrow-linewidth quasi-CW diode-pumped TEM<sub>00</sub>1064 nm Nd:YAG ring laser (2012)

We demonstrated a high average power, narrow-linewidth, quasi-CW diode-pumped Nd:YAG 1064 nm laser with near-diffraction-limited beam quality. A symmetrical three-mirror ring cavity with unidirectional operation elements and an etalon was employed to realize the narrow-linewidth laser output. Two highly efficient laser modules and a 90° quartz rotator for birefringence compensation were used for the high output power. The maximum average output power of 62.5 W with the beam quality factor M(2) of 1.15 was achieved under a pump power of 216 W at a repetition rate of 500 Hz, corresponding to the optical-to-optical conversion efficiency of 28.9%. The linewidth of the laser at the maximum output power was measured to be less than 0.2 GHz.
CO<sub>2</sub> and Formate Complexes of Phosphine/Borane Frustrated Lewis Pairs (2011)

The reaction of a solution of B(C6F4H)3 and either iPr3P or tBu3P with CO2 afforded the species R3P(CO2)B(C6F4H)3 (R=iPr (1), tBu (2)). In a similar fashion the boranes, RB(C6F5)2 (R=hexyl, cyclohexyl (Cy), norbornyl), ClB(C6F5)2, or PhB(C6F5)2 were combined with tBu3P and CO2 to give the species tBu3P(CO2)BR(C6F5)2 (R=hexyl (3), Cy (4), norbornyl (5), Cl (6), Ph (7)). Similarly, the compounds [tBu3PH][RBH(C6F5)2] (R= hexyl (8), Cy (9), norbornyl (10)) were prepared by reaction of the precursor frustrated Lewis pair (FLP) with H2. Subsequent reactions of 9 and 10 with CO2 afforded the species [((C6F5)2BR)2(μ-HCO2)][tBu3PH] (R= Cy (11), norbornyl (12)). In related chemistry, combinations of the boranes RBG(C6F5)2 (R=hexyl, Cy, norbornyl) with tBu3P treated with an equivalent of formic acid gave [(C6F5)2BR(HCO2)][tBu3PH] (R=hexyl (13), Cy (14), norbornyl (15)). Subsequent addition of an additional equivalent of borane provides a second synthetic route to 11 and 12. Crystallographic studies of compounds 2-6 and 8-14 are reported and discussed. Further understanding of the FLP complexation and activation of CO2 is provided by computational studies.
Survival of Mycobacterium paratuberculosis and preservation of immunoglobulin G in bovine colostrum under experimental conditions simulating pasteurization (1996)

Colostrum is macrophage rich and may serve as a source of M paratuberculosis infection to calves. Pasteurization of colostrum may lessen the risk of infection, but will not totally eliminate M paratuberculosis.
Underglycosylation of IgA1 Hinge Plays a Certain Role for Its Glomerular Deposition in IgA Nephropathy (1999)

This study was performed to isolate and investigate the IgA1 that could accumulate in glomeruli (glomerulophilic IgA1). IgA1 was fractionated by the electric charge and the reactivity to Jacalin. Serum IgA1 of IgA nephropathy patients was separated and fractionated using a Jacalin column and subsequent ion-exchange chromatography. The fractions were divided into three groups of relatively cationic (C), neutral (N), and anionic (A). IgA1 was also divided into Jacalin low (L), intermediate (I), and high (H) affinity fractions by serial elution using 25, 100, and 800 mM galactose. The left kidneys of Wistar rats were perfused with 2, 5, or 10 mg of each group of IgA1. The rats were sacrificed 15 min, 30 min, 3 h, or 24 h after the perfusion. The accumulation of each IgA1 in the glomeruli was then observed by immunofluorescence. The IgA1 of the fractions N and H separated by the two methods was definitely accumulated in the rat glomeruli with a similar pattern. The electrophoresis revealed that the macromolecular IgA1 was increased in fraction H compared with other fractions. Therefore, Jacalin high-affinity IgA1(fraction H) was applied on a diethylaminoethyl column and divided into electrically cationic (HC), neutral (HN), and anionic (HA). Only the asialo-Galbeta1,3GalNAc chain was identified in the fraction HN IgA1 by gas-phase hydrazinolysis. Furthermore, the IgA1 fraction was strongly recognized by peanut agglutinin, Vicia Villosa lectins, and antisynthetic hinge peptide antibody. These results indicated that the IgA1 molecules having the underglycosylated hinge glycopeptide played a certain role in the glomerular accumulation of IgA1 in IgA nephropathy.
Safe Exploration in Finite Markov Decision Processes with Gaussian Processes (2016)

In classical reinforcement learning agents accept arbitrary short term loss for long term gain when exploring their environment. This is infeasible for safety critical applications such as robotics, where even a single unsafe action may cause system failure or harm the environment. In this paper, we address the problem of safely exploring finite Markov decision processes (MDP). We define safety in terms of an a priori unknown safety constraint that depends on states and actions and satisfies certain regularity conditions expressed via a Gaussian process prior. We develop a novel algorithm, SAFEMDP, for this task and prove that it completely explores the safely reachable part of the MDP without violating the safety constraint. To achieve this, it cautiously explores safe states and actions in order to gain statistical confidence about the safety of unvisited state-action pairs from noisy observations collected while navigating the environment. Moreover, the algorithm explicitly considers reachability when exploring the MDP, ensuring that it does not get stuck in any state with no safe way out. We demonstrate our method on digital terrain models for the task of exploring an unknown map with a rover.
Purification and properties of alpha-amylase from Aspergillus oryzae ATCC 76080. (1995)

An alpha-amylase was purified from the solid cultural extract of Aspergillus oryzae ATCC 76080 by sequential steps of amylopectin affinity adsorption, DEAE-Sepharose ion-exchange chromatography and Sephacryl S-200 HR gel filtration. By these steps, the purity of the enzyme increased by 16 fold and recovery of the enzyme activity was 45%. The purified enzyme had an optimal pH between 4 to 5, optimal temperature at 50 degrees C and a Km value of 0.22% for hydrolysis of starch. About 80% of the enzyme activity was lost after incubation at 50 degrees C for 30 min. The heat denaturation constant at 50 degrees C was 0.024 min-1. The molecular weight was 52 kDa as determined by gel filtration. Mercuric ion (0.3 mM), DNFB# (6 mM), NBSI (6 mM) and NAI (6 mM) inhibited the activity of the enzyme. The main products for hydrolysis of maltoheptaose by the enzyme were maltotriose and maltotetraose.
HDAC6 Brain Mapping with [<sup>18</sup>F]Bavarostat Enabled by a Ru-Mediated Deoxyfluorination (2017)

Histone deacetylase 6 (HDAC6) function and dysregulation have been implicated in the etiology of certain cancers and more recently in central nervous system (CNS) disorders including Rett syndrome, Alzheimer's and Parkinson's diseases, and major depressive disorder. HDAC6-selective inhibitors have therapeutic potential, but in the CNS drug space the development of highly brain penetrant HDAC inhibitors has been a persistent challenge. Moreover, no tool exists to directly characterize HDAC6 and its related biology in the living human brain. Here, we report a highly brain penetrant HDAC6 inhibitor, Bavarostat, that exhibits excellent HDAC6 selectivity (>80-fold over all other Zn-containing HDAC paralogues), modulates tubulin acetylation selectively over histone acetylation, and has excellent brain penetrance. We further demonstrate that Bavarostat can be radiolabeled with <sup>18</sup>F by deoxyfluorination through <i>in situ</i> formation of a ruthenium π-complex of the corresponding phenol precursor: the only method currently suitable for synthesis of [<sup>18</sup>F]Bavarostat. Finally, by using [<sup>18</sup>F]Bavarostat in a series of rodent and nonhuman primate imaging experiments, we demonstrate its utility for mapping HDAC6 in the living brain, which sets the stage for first-in-human neurochemical imaging of this important target.
Hydrogen reduction of ilmenite: Towards an in situ resource utilization demonstration on the surface of the Moon (2019)

Water is one of the most vital resources required for future space exploration. By obtaining water from lunar regolith, humans are one step closer to being independent of Earth's resources enabling longer term exploration missions. Hydrogen reduction of ilmenite(FeTiO3) is often proposed as a technique for producing water on the Moon. ProSPA, a miniature analytical laboratory, will perform reduction of lunar soils as an In-Situ Resource Utilization (ISRU) demonstration on the lunar surface. The technique used by ProSPA will be useful for prospecting payloads with limited mass and power resources. This work considers the development and optimization of an ilmenite reduction procedure for use with the ProSPA instrument. It is shown that the reaction can be performed in a static (non-flowing) system, by utilizing a cold finger to collect the water produced from the reaction. Among the investigated parameters an initial H2:FeTiO3 ratio of 1, in this case equating to a hydrogen pressure of 418 mbar, proved to be best for providing maximum yields over 4 h when operating at 1000 °C. Results indicate that a maximum yield of 3.40 ± 0.17 wt % O2 can be obtained at 1000 °C (with a maximum possible yield of 10.5 wt % O2). When operating at higher temperatures of 1100 °C the ilmenite grains undergo a subsolidus reaction resulting in the formation of ferropseudobrookite and higher yields of 4.42 ± 0.18 wt % O2 can be obtained.
Monoclonal Antibodies to Bovine Coronavirus Glycoproteins E2 and E3: Demonstration of in vivo Virus-neutralizing Activity (1989)

Six monoclonal antibodies (MAbs) to bovine coronavirus (BCV, Quebec isolate) E2 and E3 glycoproteins which were found previously to be neutralizing in vitro were examined for virus-neutralizing activity in vivo. Surgically ligated intestinal loops of newborn colostrum-deprived calves were virus-inoculated, mock-infected or inoculated with a mixture of virus and antibody. Of the six BCV-specific MAbs, four were found to be protective against a virulent field isolate of BCV, as indicated by a reduction in villous atrophy. These MAbs were specific to antigenic domain A and antigenic domains A1 and A2 on the E2 and E3 glycoproteins respectively. MAbs to antigenic domains B and C on the E2 and E3 glycoproteins, respectively, were not protective.
SDN controller for context-aware data delivery in dynamic service chaining (2015)

Recent advances in network control and management technologies, such as software defined networking (SDN) and network function virtualization (NFV), are expected to make the network effectively able to cope with application service requirements in a more flexible and timely manner. We argue that SDN and NFV capabilities can be exploited in Next Generation Service Overlay Networks (NGSONs) for addressing contex-aware end-to-end data delivery services with adaptive composition of middlebox services when dynamically provisioned as virtual functions. In this paper we present a Service-Oriented SDN controller that allows for the programmable provision of data delivery paths through a dynamically established sequence of virtual middlebox functions, thereby accomplishing the deployment of adaptive network service chains in NGSON. In this way, an extended set of QoS requirements can be addressed that include specifications on processing functions to be traversed by service data while taking advantages from context-awareness and self-adaptation capabilities of NGSON. A use case analyis have been carried out on an experimental testbed about the effectiveness of the proposed approach in differentiating service data processing while optimizing the use of forwarding tables in the switches.