gebhart/patrepeval huggingface dataset:
Molecular Cloning and Characterization of a Novel Dual Specificity Phosphatase, MKP-5 (1999)

A group of dual specificity protein phosphatases negatively regulates members of the mitogen-activated protein kinase (MAPK) superfamily, which consists of three major subfamilies, MAPK/extracellular signal-regulated kinase (ERK), stress-activated protein kinase (SAPK)/c-Jun N-terminal kinase (JNK), and p38. Nine members of this group of dual specificity phosphatases have previously been cloned. They show distinct substrate specificities for MAPKs, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. Here we have cloned and characterized a novel dual specificity phosphatase, which we have designated MKP-5. MKP-5 is a protein of 482 amino acids with a calculated molecular mass of 52.6 kDa and consists of 150 N-terminal amino acids of unknown function, two Cdc25 homology 2 regions in the middle, and a C-terminal catalytic domain. MKP-5 binds to p38 and SAPK/JNK, but not to MAPK/ERK, and inactivates p38 and SAPK/JNK, but not MAPK/ERK. p38 is a preferred substrate. The subcellular localization of MKP-5 is unique; it is present evenly in both the cytoplasm and the nucleus. MKP-5 mRNA is widely expressed in various tissues and organs, and its expression in cultured cells is elevated by stress stimuli. These results suggest that MKP-5 is a novel type of dual specificity phosphatase specific for p38 and SAPK/JNK.
Design of FRET-TaqMan probes for multiplex real-time PCR using an internal positive control (2009)

The multiplexing capabilities with different fluorescent dyes are limited in real-time PCR instruments equipped with one excitation source. Considering this limitation, a design was developed to create a triple-labeled probe as an internal positive control (IPC) that utilizes a combination of the fluorescence resonance energy transfer (FRET) and TaqMan techniques. The IPC probe, labeled with FAM and Cy5.5 fluorophores at the 5' end and Black Hole Quencher (BHQ) at the 3' end, enabled Cy5.5 emission through energy transfer from the FAM fluorophore. The second, target-specific TaqMan assay in the multiplex used a FAM- and BHQ1-labeled probe at the 5' and 3' ends, respectively. Thus, one excitation source was used to generate two different fluorescence emissions (FAM and Cy5.5) that were measured in two separate channels by the real-time PCR instrument. This method can facilitate the development of a low-cost portable handheld real-time PCR instrument capable of multiplex real-time PCR assays using a single excitation source.
Segmentation from a box (2011)

Drawing a box around an intended segmentation target has become both a popular user interface and a common output for learning-driven detection algorithms. Despite the ubiquity of using a box to define a segmentation target, it is unclear in the literature whether a box is sufficient to define a unique segmentation or whether segmentation from a box is ill-posed without higher-level (semantic) knowledge of the intended target. We examine this issue by conducting a study of 14 subjects who are asked to segment a boxed target in a set of 50 real images for which they have no semantic attachment. We find that the subjects do indeed perceive and trace almost the same segmentations as each other, despite the inhomogeneity of the image intensities, irregular shapes of the segmentation targets and weakness of the target boundaries. Since the subjects produce the same segmentation, we conclude that the problem is well-posed and then provide a new segmentation algorithm from a box which achieves results close to the perceived target.
Emerging wireless broadband networks (1998)

The rapid evolution of mobile wireless access networks toward multimedia support with QoS provision forces the development of advanced wireless broadband systems with high reliability and high data rate. To achieve this goal, new system design concepts with increased system capacity will be required. In that context, ATM is becoming a major infrastructure, receiving a lot of attention for telecommunication systems since ATM networks can most effectively support wireless access systems. Wireless ATM systems have low transmission cost, flexible functionality, mobile ATM protocol, and radio access layer protocols. This article overviews the various wireless broadband systems studied at 5, 19.37, 40, and 60 GHz by European Union funded projects within the ACTS program. Moreover, related standardization activities and network evolution are also addressed.
Abuse-Deterrent Formulations and the Prescription Opioid Abuse Epidemic in the United States (2015)

Abuse-deterrent formulations can have the intended purpose of curtailing abuse, but the extent of their effectiveness has clear limits, resulting in a significant level of residual abuse. Consequently, although drug abuse policy should focus on limiting supplies of prescription analgesics for abuse, including ADF technology, efforts to reduce supply alone will not mitigate the opioid abuse problem in this country.
Roadmap for densification in cold sintering: Chemical pathways (2020)

The cold sintering process (CSP) is a technique that enables densification of ceramics and composites at extremely low temperatures (T ​< ​400 ​°C) with the application of a uniaxial pressure and a transient solvent. At the laboratory scale, comparing direct sintering methods relative to CSP evidences that the latter could enable a sustainable eco-manufacturing path with considerable energy and emission savings, as well as fast processing times. Thus far, cold sintering has been applied to different materials, compounds, solid solutions, and functional composites, pointing to multiple future opportunities. This paper summarizes some of the transient phase selections, different chemical approaches and subtle chemical reactions with the powders which can be used to drive the cold sintering processes in many important ceramics and composites. In doing so, this paper highlights some of the reasons why the fundamental understanding of CSP mechanisms remains challenging, but classification of the pathways as outlined here should aid progress towards a more comprehensive understanding.
Co‐assessment of cytoplasmic and nuclear androgen receptor location in prostate specimens: potential implications for prostate cancer development and prognosis (2008)

We propose that increased nuclear AR expression might be a precursor to PC and that cytoplasmic AR could contribute to the AIPC phenotype. The predictive ability of the AR might be closely linked to clinicopathological features.
SpectreGuard (2019)

Speculative execution is an essential performance enhancing technique in modern processors, but it has been shown to be insecure. In this paper, we propose SpectreGuard, a novel defense mechanism against Spectre attacks. In our approach, sensitive memory blocks (e.g., secret keys) are marked using simple OS/library API, which are then selectively protected by hardware from Spectre attacks via low-cost micro-architecture extension. This technique allows microprocessors to maintain high performance, while restoring the control to software developers to make security and performance trade-offs.
Rodents: soft tissue surgery (2009)

Surgical magnification is very important because of the small patient size. Loss of relatively small volumes of blood can have serious consequences, including death. It is often not feasible to intubate them for anaesthesia and vital signs routinely monitored in larger patients (such as blood pressure and oxygen tension) often cannot be evaluated accurately in these small pets. Common conditions and techniques are detailed, such as Gonadectomy/neutering; Uterine diseases; Mammary glands; Enucleation and exenteration; Cystotomy; Tail degloving; and Everted cheek pouch.
Underdevelopment of the postural control system in autism (2004)

The evidence from this and studies of the motor system suggests more general involvement of neural circuitry beyond the neural systems for social behavior, communication, and reasoning, all of which share a high demand on neural integration of information.
Structural Performance Analysis of New Type CFTA Girder Bridge (2011)

본 논문에서는 아치 형상을 갖는 강재 거더에 콘크리트가 충진되고 외부에 긴장력을 도입하여 구조적 효율성을 높인 CFTA 거더(Concrete-Filled and Tied Steel Tubular Arch Girder)의 아치효과와 구조적 성능을 검증하기 위해 정적하중재하실험을 수행하였고, 실험결과의 타당성을 검증하기 위해 유한요소해석을 수행하였다. CFTA 거더는 강재, 콘크리트, PS강재 등을 이용하여 각 재료의 역학적 장점을 최대한 발휘하도록 형상화하였다. 본 연구에서는 프레임해석프로그램으로 지간장 12m인 축소모형을 설계하였으며, 해석결과에 근거하여 정적재하실험이 수행되었다. 정적재하실험 결과, CFTA 거더의 구조적 성능과 안전성이 검증되었으며, 아치 효과에 의해 타 구조형식과는 다른 처짐 형상이 나타났다. 또한 ABAQUS 프로그램을 이용한 유한요소해석을 통해 차량 충돌 안정성과 정적재하실험결과의 타당성을 입증하였다. In this research, static load test is performed to verify the arch effect and structural performance of CFTA(Concrete-Filled and Tied steel tubular Arch) girder, and FE(Finite Element) analysis is performed to investigate validity of the test result. CFTA girder is designed to maximize the benefit of each material, such as steel plate, filled concrete and PS tendon. Static load test is performed based on the frame-analysis result of 12m sample miniature model. The result of static load test is that structural performance and safety of CFTA girder are confirmed and there is different deflection mode with other structural form result from arch effect. FE analysis with ABAQUS is also performed to show the validity of the truck collision safety and static load test.
Visualization of the Boron Neutron Capture Therapy treatment planning process (2015)

The focus of this thesis is the development of a visualization module (Sera3d) for the Simulated Environment for Radiotherapy Applications (SERA) software package, under development at Montana State University and the Idaho National Engineering and Environmental Laboratories. In 1997, a highly efficient particle tracking method was developed to speed the Monte Carlo transport calculations, which simulate the neutron/boron interactions in the anatomical geometry. The new particle tracking method required a uniform volume element (univel) description of the geometry. The uniformity allowed for fast tracking through highly efficient indexing schemes and integer-based arithmetic operations. The adoption of the univel descriptions resulted in the restructuring of the software package. With the restructuring of the older software package into SERA, and the advances in computer graphics hardware, the addition of a visualization module was undertaken to provide a visual understanding of all aspects of the proposed treatment plan. The three dimensional rendering in Sera3d is built on the OpenGL graphics library, while all of the user interfaces and components were developed using the Motif toolkit in an X11 window environment.
Oxidation of an NiTi alloy (1990)

Abstract In this study, the oxidation of the surface of a 50/50 (at.%) NiTi alloy was studied by XPS, AES and SEM. Samples of the NiTi alloy were oxidized at 23°C and high temperatures at an oxygen pressure of 10 −4 Torr and in atmosphere. At 10 −4 Torr of O 2 and 23°C, titanium was oxidized preferentially while nickel remained in the metallic form even after 40 min of exposure. At the same oxygen partial pressure and 400°C, a mixture of TiO x , Ti 2 O 3 and TiO 2 was formed, and nickel remained metallic. Eventually, after 40 min of O 2 exposure, the whole surface was covered with a layer of TiO 2 . In atmosphere and at 23°C, titanium again was oxidized preferentially, but NiO was detected on the surface after the first minute of air exposure. At a higher temperature of 450°C, areas comprising a mixture of NiO and TiO 2 , and areas consisting of TiO 2 only were found. Below the top surface layer, a complete layer of TiO 2 was detected. Underneath the TiO 2 layer, a nickel‐rich layer was present.
Robust channel identification scheme: Solving permutation indeterminacy of ICA for artifacts removal from ECG (2010)

In this paper we propose a novel channel identification scheme for solving permutation indeterminacy introduced by Independent Component Analysis (ICA) for artifacts removal from recorded three channel ECG signals within the remote health monitoring environment. The proposed scheme does not depend on the definition of any specific artifact which is the case with the existing approach and therefore leads to more robust and generic solution to this problem. The proposed scheme has been validated using nine practical case studies and its robustness has been proved by comparison with the existing approach. Simulation results show that the proposed scheme works successfully for all the nine cases whereas the existing approach fails to identify the correct channel for four cases.
Molten salt lithium cells (2023)

Lithium-based cells are promising for applications such as electric vehicles and load-leveling for power plants since lithium is very electropositive and light weight. One type of lithium-based cell utilizes a molten salt electrolyte and is operated in the temperature range of about 400.degree.-500.degree. C. Such high temperature operation accelerates corrosion problems and a substantial amount of energy is lost through heat transfer. The present invention provides an electrochemical cell (10) which may be operated at temperatures between about 100.degree.-170.degree. C. Cell (10) comprises an electrolyte (16), which preferably includes lithium nitrate, and a lithium or lithium alloy electrode (12).
Synthesis of new exceedingly strong non-ionic bases: RN:P(MeNCH2CH2)3N (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of new exceedingly strong non-ionic bases: RN:P(MeNCH2CH2)3NJiansheng Tang, Joel Dopke, and John G. VerkadeCite this: J. Am. Chem. Soc. 1993, 115, 12, 5015–5020Publication Date (Print):June 1, 1993Publication History Published online1 May 2002Published inissue 1 June 1993https://pubs.acs.org/doi/10.1021/ja00065a009https://doi.org/10.1021/ja00065a009research-articleACS PublicationsRequest reuse permissionsArticle Views817Altmetric-Citations91LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (2)»Supporting Information Supporting Information Get e-Alerts
CCL2 Is a Negative Regulator of AMP-Activated Protein Kinase to Sustain mTOR Complex-1 Activation, Survivin Expression, and Cell Survival in Human Prostate Cancer PC3 Cells (2009)

CCL2 is a cytokine prevalent in the prostate cancer tumor microenvironment. Recently, we reported that CCL2 induces the mammalian target of rapamycin (mTOR) pathway to promote prostate cancer PC3 cell survival; however, the mechanism used by CCL2 to maintain mTOR complex-1 (mTORC1) activation requires clarification. This study demonstrates that upon serum starvation, CCL2 functions as a negative regulator of AMP-activated protein kinase (AMPK) by decreasing phosphorylation at its major regulatory site (Thr(172)) in PC3, DU145, and C4-2B prostate cancer cells. The CCL2-mediated AMPK regulation decreased raptor phosphorylation (Ser(792)) resulting in hyperactivation of mTORC1. D942, a pharmacological activator of AMPK, stunted CCL2-induced mTORC1 activity, survivin expression, and cell survival without significantly affecting Akt activity. CCL2, however, conferred some resistance to the lethal effect of D942 compared with untreated cells. By using Akt-specific inhibitor X, it was shown that Akt inactivation did not cause an increase in AMPK phosphorylation in CCL2-stimulated cells, suggesting that CCL2-mediated negative regulation of AMPK is independent of Akt. Furthermore, bisindolylmaleimide-V, a specific inhibitor of p70(S6K), stunted survivin expression and induced cell death in CCL2-treated PC3. Altogether, these findings suggest that CCL2 hyperactivates mTORC1 through simultaneous regulation of both AMPK and Akt pathways and reveals a new network that promotes prostate cancer: CCL2-AMPK-mTORC1-survivin.
Catalytic Dehydrogenation of Light Alkanes on Metals and Metal Oxides (2014)

A study is conducted to demonstrate catalytic dehydrogenation of light alkanes on metals and metal oxides. The study provides a complete overview of the materials used to catalyze this reaction, as dehydrogenation for the production of light olefins has become extremely relevant. Relevant factors, such as the specific nature of the active sites, as well as the effect of support, promoters, and reaction feed on catalyst performance and lifetime, are discussed for each catalytic Material. The study compares different catalysts in terms of the reaction mechanism and deactivation pathways and catalytic performance. The duration of the dehydrogenation step depends on the heat content of the catalyst bed, which decreases rapidly due to the endothermic nature of the reaction. Part of the heat required for the reaction is introduced to the reactors by preheating the reaction feed, additional heat being provided by adjacent reactors that are regenerating the coked catalysts.
Design of Location-based Directory Facilitator in Context-Aware Environment (2008)

In ubiquitous computing environments, the basic information such as types or locations of services should be provided in advance to use distributed services and devices efficiently. Using a combination of developed agent technology and service discovery to efficiently and flexibly manage distributed services, devices, and users is a trend. In this paper, we analyze the DF's searching method and designed ubiquitous location-based service in context-aware environment, because context-aware environment provides a suitable method to gather requested information in an appropriate way.
Heavy-Metal and Benzalkonium Chloride Resistance of <i>Listeria monocytogenes</i> Isolates from the Environment of Turkey-Processing Plants (2008)

The resistance of Listeria monocytogenes to cadmium and arsenic has been used extensively for strain subtyping. However, limited information is available on the prevalence of such resistance among isolates from the environment of food-processing plants. In addition, it is not known whether the resistance of such isolates to heavy metals may correlate with resistance to quaternary ammonium compounds extensively used as disinfectants in the food-processing industry. In this study, we characterized 192 L. monocytogenes isolates (123 putative strains) from the environment of turkey-processing plants in the United States for resistance to cadmium and arsenic and to the quaternary ammonium disinfectant benzalkonium chloride (BC). Resistance to cadmium was significantly more prevalent among strains of serotypes 1/2a (or 3a) and 1/2b (or 3b) (83% and 74%, respectively) than among strains of the serotype 4b complex (19%). Resistance to BC was encountered among 60% and 51% of the serotype 1/2a (or 3a) and 1/2b (or 3b) strains, respectively, and among 7% of the strains of the serotype 4b complex. All BC-resistant strains were also resistant to cadmium, although the reverse was not always the case. In contrast, no correlation was found between BC resistance and resistance to arsenic, which overall was low (6%). Our findings suggest that the processing environment of turkey-processing plants may constitute a reservoir for L. monocytogenes harboring resistance to cadmium and to BC and raise the possibility of common genetic elements or mechanisms mediating resistance to quaternary ammonium disinfectants and to cadmium in L. monocytogenes.
Hemodynamics and the vascular endothelial cytoskeleton. (1987)

Although there is considerable evidence to suggest that hemodynamics play an important role in vascular disease processes, the exact mechanisms are unknown. With this in mind, we have designed a pulsatile perfusion apparatus which reproducibly delivers pulsatile hemodynamics upon freshly excised canine carotid arteries in vitro. Quantifiable simulations included normotension with normal or lowered flow rates (120/80 mmHg, 120 and 40 ml/min), normotension with lowered or elevated transmural pressures (40-170 mmHg), and elevated pulse pressure (120 and 80 mmHg) with normal (150 ml/min) or elevated rates of flow (300 and 270 ml/min). Arterial biomechanical stresses and cellular behaviors were characterized biochemically and morphologically under all these stimulations which continued for 2-24 h. We found that increased pulse pressure alone had little effect on the total amount of radiolabeled [4-14C]cholesterol present within the medial compartment. However, normotension when coupled with altered transmural pressure yielded a three- to fourfold increase. Combinations of increased pulse pressure and flow potentiated cholesterol uptake by a factor of 10 when compared with normotension control values. Simulations that enhanced carotid arterial cholesterol uptake also influenced the endothelial cytoskeletal array of actin. Stress fibers were not present within the carotid endothelial cells of either the sham controls or the normotension and increased pulse pressure (normal flow) simulations. Endothelial cells lining carotids exposed to elevations in flow or those present within vessels perfused as per simulation b above assembled stress fibers (x = 4 and 10 per cell, respectively) within the time course of these studies. When endothelial cells were subjected to hemodynamic conditions that potentiated maximally cholesterol transport, no diffuse or stress fiber staining could be seen, but the cortical array of actin was intact. These results suggest that those biomechanical stresses that alter endothelial permeability and intimal integrity may do so via cytoskeletal actin signaling.
Local Saline Infusion into Ischemic Territory Induces Regional Brain Cooling and Neuroprotection in Rats with Transient Middle Cerebral Artery Occlusion (2004)

Local prereperfusion infusion effectively induced hypothermia and ameliorated brain injury from stroke. Clinically, this procedure could be used in acute stroke treatment, possibly in combination with intra-arterial thrombolysis or mechanical disruption of clot by means of a microcatheter.
Non-invasive rapid and efficient firmware update for wireless sensor networks (2014)

To maintain software of sensor nodes in wireless sensor networks efficiently, it is necessary to minimize the size of transferred data in firmware update. We propose a non-invasive rapid and efficient incremental firmware update algorithm called MoRE. In MoRE algorithm, the host transfers only delta, which is the information of different parts between old and new firmware image, to reduce the size of transferred data. The sensor node makes new binary image from its current image and the transferred messages. The MoRE shows comparable performance to previous works without invasive methods. Unlike the previous works, MoRE does not require extra memory for metadata in sensor nodes and does not need to use relocatable code.