gebhart/patrepeval huggingface dataset:
Cloning of dapD, aroD and asd of Leptospira interrogans serovar icterohaemorrhagiae, and nucleotide sequence of the asd gene (1992)

Metabolites such as diaminopimelate and some aromatic derivatives, not synthesized in mammalian cells, are essential for growth of bacteria. As a first step towards the design of a new human live vaccine that uses attenuated strains of Leptospira interrogans, the asd, aroD and dapD genes, encoding aspartate beta-semialdehyde dehydrogenase, 3-dehydroquinase and tetrahydrodipicolinate N-succinyltransferase, respectively, were cloned by complementation of Escherichia coli mutants. The complete nucleotide sequence of the asd gene was determined and found to contain an open reading frame capable of encoding a protein of 349 amino acids with a calculated Mr of 38,007. Comparison of this deduced L. interrogans aspartate beta-semialdehyde dehydrogenase amino acid sequence with those of the same enzyme from Saccharomyces cerevisiae and Corynebacterium glutamicum revealed 46% and 36% identity, respectively. By contrast, the identity between the L. interrogans enzyme and the Streptococcus mutans or E. coli enzymes was less than 31%. Highly conserved sequences within aspartate semialdehyde dehydrogenase from the five organisms were observed at the amino and carboxyl termini, and around the cysteine of the active site.
First Fifty Years of Chemoresistive Gas Sensors (2015)

The first fifty years of chemoresistive sensors for gas detection are here reviewed, focusing on the main scientific and technological innovations that have occurred in the field over the course of these years. A look at advances made in fundamental and applied research and leading to the development of actual high performance chemoresistive devices is presented. The approaches devoted to the synthesis of novel semiconducting materials with unprecedented nanostructure and gas-sensing properties have been also presented. Perspectives on new technologies and future applications of chemoresistive gas sensors have also been highlighted.
The position 4 nucleotide at the 3' end of the influenza virus neuraminidase vRNA is involved in temporal regulation of transcription and replication of neuraminidase RNAs and affects the repertoire of influenza virus surface antigens. (1998)

Within the sequence motif conserved at the extreme ends of the influenza virus vRNAs, a unique natural variation, U or C, is observed at position 4 of the 3' end. To test the role of this nucleotide, two isogenic A/WSN/33 viruses, carrying either C4 or U4 nucleotide at the 3' end of the neuraminidase (NA) gene, were generated. Compared with the C4 virus, the U4 virus exhibited delayed synthesis of vRNA and stimulation of mRNA synthesis with prolonged accumulation in influenza virus-infected cells. The mRNA/ vRNA ratio was increased up to 20-fold by the C4 --> U4 substitution suggesting that the U4 nucleotide greatly stimulated transcription of the vRNA template. In isolated virion, the U4 virus had higher NA activity than the C4 virus. In MDBK cells, the U4 virus grew to lower haemagglutination (HA) titres but with higher infectivity than the C4 virus, with a corresponding increase in the ratio of p.f.u./HA units of about 10- to 40-fold. Western blot analysis of isolated virion showed that the ratio of two surface proteins, HA/NA, was greatly decreased in the U4 virus. This suggests that the position 4 nucleotide is a genetic determinant for the repertoire of surface antigens and their ratio could be changed without detrimental effects on virus growth. Results could be used to design genetically engineered influenza virus for vaccination. The observed down-regulation of transcription by C4 nucleotide is consistent with its potential role in segment-specific regulation of influenza virus gene expression, especially PB1, PB2 and PA proteins, during virus infection.
Loop shaping H/sub ∞/ design applied to the steam generator level control in EDF nuclear power plants (2002)

The objective of this paper is to design a controller for a pressurized water reactor steam generator using model based control techniques. The loop-shaping design procedure of McFarlane and Glover (1992) is used together with the gap metric of Vinnicombe (1992), the latter allowing one to a priori insure the robust stability of several linearized models. Namely the gap metric is used to select the nominal model of a family P of models and to select the design parameters of the loop-shaping procedure. Robust stability against parametric uncertainties are investigated with real /spl mu/-analysis. The classical approach of frequency gridding is compared with a method of elimination of frequency search proposed by Sideris and Helmersson. The proposed controller is finally validated on a nonlinear PWR model of a nuclear power plant.
Iontoforese no transporte ocular de drogas (2004)

O método mais comum de administração de drogas no olho é por meio de colírios. Entretanto, por este método, não é possível atingir a concentração terapêutica nos fluidos e tecidos posteriores do olho. A administração sistêmica apresenta reduzido acesso ao segmento posterior do olho devido à presença das barreiras oculares. Injeções subconjuntivais e retrobulbares não são capazes de proporcionar níveis adequados da droga, e a injeção intravítrea é método invasivo, inconveniente e que apre-senta riscos de perfuração do bulbo ocular ou descolamento da retina. A iontoforese, no entanto, apresenta-se como alternativa para o transporte de doses terapêuticas de drogas para o segmento posterior do olho. A iontoforese é uma técnica que consiste na administração de drogas para o organismo através dos tecidos, utilizando um campo elétrico. O eletrodo ativo, que se encontra em contato com a droga, é colocado no local a ser tratado, e um segundo eletrodo, com a finalidade de fechar o circuito elétrico, é colocado em outro local do organismo. O campo elétrico facilita o transporte da droga, que deve se encontrar, preferencialmente, na forma ionizada. A iontoforese pode ser considerada como um método seguro e não invasivo de transporte de drogas para locais específicos do olho. Aplicada experimentalmente para o tratamento de doenças oculares, esta técnica tem evoluído muito nos últimos anos e, atualmente, testes clínicos de fase III encontram-se em andamento.
Supply chain resilience: a systematic literature review and typological framework (2018)

Purpose The study of supply chain resilience (SCRES) continues to gain interest in the academic and practitioner communities. The purpose of this paper is to present a focused review of the SCRES literature by investigating supply chain (SC) capabilities, their relationship to SCRES outcomes and the underpinning theoretical mechanisms of this relationship. Design/methodology/approach The paper uses the systematic literature review approach to examine 383 articles published between 2000 and 2017, ultimately down selecting to the most relevant 228 peer-reviewed studies. Context-interventions-mechanisms-outcomes (CIMO) logic is applied to organize and synthesize these peer-reviewed studies. A typological framework is developed from the CIMO-based classification of the SCRES literature. Findings The findings of this study outline the gaps in the SCRES literature and present an agenda for future research. Research limitations/implications This paper presents an exploratory research; therefore, the typological model presented is just one of the possible perspectives. Practical implications The typology of SCRES literature can help practitioners to understand SCRES and to measure and assess the resilience of SCs. Originality/value The paper provides clear definitions of SCRES constructs, develops a typological framework to further understand SCRES and identifies SCRES measures and assessment techniques.
Taking Nanocasting One Step Further: Replicating CMK-3 as a Silica Material (2002)

Casting silica nets: By repeated “nanocasting” it is possible to first obtain a negative of the SBA-15 structure, known as CMK-3, which is then used to produce another nanocast by impregnation with tetraethoxysilane and condensation. After calcination to remove the carbon, an ordered mesoporous silica is obtained, called NCS-1 (see scheme). This pathway could be generalized to allow also the synthesis of other ordered oxides.
Radiation Hardness of Scintillation Detectors Based on Organic Plastic Scintillators and Optical Fibers (2019)

Scintillation detectors (SD) based on organic plastic scintillators and optical fibers are basic detectors at all modern accelerators and in astrophysics and neutrino physics experiments. In recent years, interest in SD has significantly increased due to the upcoming major upgrade of the LHC and the construction of new accelerators NICA, FAIR, FCC, etc. At the same time, requirements on the stability and reliability of SD operation under new conditions have become stricter, and their fulfillment largely depends on radiation hardness of scintillators, optical fibers, and photodetectors. The review presents the results of the radiation hardness investigations of various scintillators, optical fibers (scintillating, as well as wavelength-shifting and clear), and optical glues used to increase light collection from scintillators by fibers. The influence of various factors (dose, dose rate, type of radiation, scintillator materials, and fluors) on light output, light collection, and transmittance of the irradiated materials and their recovery is considered. Aging of scintillators caused by environmental effects (temperature, humidity) irrespective of radiation is also briefly considered.
Nomenclature of the tourmaline-supergroup minerals (2011)

A nomenclature for tourmaline-supergroup minerals is based on chemical systematics using the generalized tourmaline structural formula: XY 3Z6(T6O18)(BO3) 3V3W, where the most common ions (or vacancy) at each site are X = Na1+, Ca2+, K1+, and vacancy; Y = Fe2+, Mg2+, Mn2+, Al3+, Li 1+, Fe3+, and Cr3+; Z = Al3+, Fe3+, Mg2+, and Cr3+; T = Si4+, Al3+, and B3+; B = B3+; V = OH1- and O2-; and W = OH1-, F1-, and O2-. Most compositional variability occurs at the X, Y, Z, W, and V sites. Tourmaline species are defined in accordance with the dominant-valency rule such that in a relevant site the dominant ion of the dominant valence state is used for the basis of nomenclature. Tourmaline can be divided into several groups and subgroups. The primary groups are based on occupancy of the X site, which yields alkali, calcic, or X-vacant groups. Because each of these groups involves cations (or vacancy) with a different charge, coupled substitutions are required to relate the compositions of the groups. Within each group, there are several subgroups related by heterovalent coupled substitutions. If there is more than one tourmaline species within a subgroup, they are related by homovalent substitutions. Additionally, the following considerations are made. (1) In tourmaline-supergroup minerals dominated by either OH1- or F 1- at the W site, the OH1--dominant species is considered the reference root composition for that root name: E.g., dravite. (2) For a tourmaline composition that has most of the chemical characteristics of a root composition, but is dominated by other cations or anions at one or more sites, the mineral species is designated by the root name plus prefix modifiers, e.g., fluor-dravite. (3) If there are multiple prefixes, they should be arranged in the order occurring in the structural formula, e.g., "potassium-fluor- dravite.
Expression of a mannosyl-fucosyl receptor for endocytosis on cultured primary macrophages and their hybrids. (1982)

The presence of a pinocytosis receptor, specific for mannose-fucose terminated glycoproteins, has been established on murine resident peritoneal macrophages, thioglycollate-elicited peritoneal macrophages, and macrophages derived from bone-marrow in culture. Macrophagelike cell lines (J-774 and P338.D1), a myelomonocytic cell line (427E), lymphocytes, polymorphonuclear leukocytes, and fibroblasts were negative. Binding and uptake of 125I-mannose-BSA and 125I-beta-glucuronidase, respectively, into thioglycollate-induced peritoneal macrophages is saturable (Kd 4 degrees C = 5.4 X 10(-9) M; Kuptake 37 degrees C = 7 X 10(-7) M) and sugar specific. Macrophage-macrophage (rat X mouse) hybrids prepared by fusing rat alveolar macrophages with J-774-B10 (HAT-sensitive macrophagelike cell line) expresses the mannose-fucose receptor. Karyotypes of the hybrids confirmed a 1:1 fusion of rat and mouse cells. The rat/mouse hybrids express a variety of rat and mouse antigens including Fc receptors. Fibroblast-macrophage hybrids and melanoma-macrophage hybrids were negative for mannose-fucose receptor activity. The expression of the mannose-fucose receptor by macrophages appears to be regulated independently of other macrophage markers.
A novel virtual machine based approach for hosting NFC services on mobile devices (2010)

NFC is one of the latest wireless communication technologies, making possible variety of business opportunities. At Budapest University of Technology and Economics (BME) a generalized approach for NFC application development has been elaborated, where the Mobile Network Operators (MNOs), as well as service providers can make their own application and run it in the proposed common environment. The developed a virtual machine like application execution framework gives the opportunity of rapid implementation of any kind of business logic and user interface for NFC applications and provides high level of application security. The current paper presents the developed novel NFC application development approach, the applied technical solutions and the implemented script execution environment running on a mobile device.
Effect of Genomic Integration Location on Heterologous Protein Expression and Metabolic Engineering in <i>E. coli</i> (2017)

Chromosomal integration offers a selection-free alternative to DNA plasmids for expression of foreign proteins and metabolic pathways. Episomal plasmid DNA is convenient but has drawbacks including increased metabolic burden and the requirement for selection in the form of antibiotics. E. coli has long been used for the expression of foreign proteins and for the production of valuable metabolites by expression of complete metabolic pathways. The gene encoding the fluorescent reporter protein mCherry was integrated into four genomic loci on the E. coli chromosome to measure protein expression at each site. Expression levels ranged from 25% to 500% compared to the gene expressed on a high-copy plasmid. Modular expression of DNA is one of the most commonly used methods for optimizing metabolite production by metabolic engineering. By combining a recently developed method for integration of large synthetic DNA constructs into the genome, we were able to integrate two foreign pathways into the same four genomic loci. We have demonstrated that only one of the genomic loci resulted in the production of violacein, and that all four loci produced trans-cinnamic acid from the TAL pathway.
Multi-mode controller IC for soft-switched flyback converter with high efficiency over the entire load range (2008)

This paper presents a multi-mode control scheme for a soft-switched flyback converter to achieve high efficiency and excellent load regulation over the entire load range. At heavy load, critical conduction mode with valley switching (CCMVS) is employed to realize soft switching so as to reduce turn-on loss of power switch as well as conducted electromagnetic interference (EMI). At light load, the converter operates in discontinuous conduction mode (DCM) with valley switching and adaptive off-time control (AOT) to limit the switching frequency range and maintain load regulation. At extremely light load or in standby mode, burst mode operation is adopted to provide low power consumption through reducing both switching frequency and static power dissipation of the controller. The multi-mode control is implemented by an oscillator whose pulse duration is adjusted by output feedback. An accurate valley switching control circuit guarantees the minimum turn-on voltage drop of power switch. The prototype of the controller IC was fabricated in a 1.5-μm BiCMOS process and applied to a 310 V/20 V, 90 W flyback DC/DC converter circuitry. Experimental results showed that all expected functions were realized successfully. The flyback converter achieved a high efficiency of over 80% from full load down to 2.5 W, with the maximum reaching 88.8%, while the total power consumption in standby mode was about 300 mW.
Issues Relating to Long-term, Out-of-home Care for Children of Parents with Mental Illness (2004)

This section focuses on the dilemmas that face the child welfare system and the court system in ensuring that when children of mentally ill parents are placed away from their families, the needs of the children and their families can best be met. Emphasis is given to the situation in which the practice principles of 'permanency' planning are central to the philosophy of case-planning.
Reactivity of Lithium toward Propylene Carbonate:  Infrared Reflection Absorption Spectroscopy Studies in Ultrahigh Vacuum (2001)

The vibrational properties of vapor-deposited lithium films exposed to propylene carbonate (PC) vapors at room temperature have been examined by infrared reflection−absorption spectroscopy (IRAS) in ultrahigh vacuum (UHV) using a specially designed chamber. The sharply defined spectral features observed were found to be incompatible with those of either, lithium propoxide, lithium alkyl carbonate, lithium carbonate, or mixtures thereof. Instead, the IRAS spectrum of PC/Li/Ni was found to be identical to that obtained by exposing Li to 1,2-propanediol vapors under otherwise identical conditions and may thus be attributed to the corresponding alkoxide derivative.
SPRINTER: A Discrete Locomotion Robot for Precision Swarm Printing (2019)

This paper presents SPRINTER, a system and method for multi-robot printing. In this paper, we discuss the design of a quasi-holonomic mobile robot and present a method which uses a group of such robots to distributively print a large graphical image. In the distributive printing method, we introduce the concept of image cellularization for segmenting the graphic into a group of smaller printing tasks. We then discuss a centralized method to allocate these tasks to each robot and execute the printing process. In summary, we present a multi-robot printing system which enhances the printing speed and maximizes the printing area of traditional industrial printers.
Functional Characterization of Novel Faecalibacterium prausnitzii Strains Isolated from Healthy Volunteers: A Step Forward in the Use of F. prausnitzii as a Next-Generation Probiotic (2017)

<i>Faecalibacterium prausnitzii</i> is a major member of the Firmicutes phylum and one of the most abundant bacteria in the healthy human microbiota. <i>F. prausnitzii</i> depletion has been reported in several intestinal disorders, and more consistently in Crohn's disease (CD) patients. Despite its importance in human health, only few microbiological studies have been performed to isolate novel <i>F. prausnitzii</i> strains in order to better understand the biodiversity and physiological diversity of this beneficial commensal species. In this study, we described a protocol to isolate novel <i>F. prausnitzii</i> strains from feces of healthy volunteers as well as a deep molecular and metabolic characterization of these isolated strains. These <i>F. prausnitzii</i> strains were classified in two phylogroups and three clusters according to 16S rRNA sequences and results support that they would belong to two different genomospecies or genomovars as no genome sequencing has been performed in this work. Differences in enzymes production, antibiotic resistance and immunomodulatory properties were found to be strain-dependent. So far, all <i>F. prausnitzii</i> isolates share some characteristic such as (i) the lack of epithelial cells adhesion, plasmids, anti-microbial, and hemolytic activity and (ii) the presence of DNAse activity. Furthermore, Short Chain Fatty Acids (SCFA) production was assessed for the novel isolates as these products influence intestinal homeostasis. Indeed, the butyrate production has been correlated to the capacity to induce IL-10, an anti-inflammatory cytokine, in peripheral blood mononuclear cells (PBMC) but not to the ability to block IL-8 secretion in TNF-α-stimulated HT-29 cells, reinforcing the hypothesis of a complex anti-inflammatory pathway driven by <i>F. prausnitzii</i>. Altogether, our results suggest that some <i>F. prausnitzii</i> strains could represent good candidates as next-generation probiotic.
Applying robot-in-the-loop-simulation to mobile robot systems (2006)

Simulation-based study plays important roles in robotic systems development. This paper describes robot-in-the-loop simulation, which is a novel simulation method that we developed to allow real robots and robot models to work together for system-wide measurement and test. To support robot-in-the-loop simulation, a simulated virtual environment is developed. Several kinds of sensor/actuator interface models are defined to allow real robots to interact with the virtual environment. To demonstrate the capability of robot-in-the-loop simulation, three mobile robot examples were developed and are presented in the paper in an incremental way. These examples illustrate the usages and configurations of robot-in-the-loop simulation under different situations
Implicit and Explicit Concept Relations in Deep Neural Networks for Multi-Label Video/Image Annotation (2018)

In this paper, we propose a deep convolutional neural network (DCNN) architecture that addresses the problem of video/image concept annotation by exploiting concept relations at two different levels. At the first level, we build on ideas from multi-task learning, and propose an approach to learn concept-specific representations that are sparse, linear combinations of representations of latent concepts. By enforcing the sharing of the latent concept representations, we exploit the implicit relations between the target concepts. At a second level, we build on ideas from structured output learning and propose the introduction, at training time, of a new cost term that explicitly models the correlations between the concepts. By doing so, we explicitly model the structure in the output space (i.e., the concept labels). Both of the above are implemented using standard convolutional layers and are incorporated in a single DCNN architecture that can then be trained end-to-end with standard back-propagation. Experiments on four large-scale video and image data sets show that the proposed DCNN improves concept annotation accuracy and outperforms the related state-of-the-art methods.
A New Miniaturized Electronically Tunable Bandpass Filter (2006)

In this paper a new electronically tunable band pass filter using coupled ring resonator is described. This filter can tune frequency ranges from 2 GHz to 4 GHz. In comparison with designed filter by Y.H.SHu the size of this filter reduced about 69% with the same frequency performance.