gebhart/patrepeval huggingface dataset:
Human Fusobacterium necrophorum strains have a leukotoxin gene and exhibit leukotoxic activity (2008)

Fusobacterium necrophorum, a Gram-negative anaerobe, causes a variety of necrotic infections in humans and animals. There are two subspecies: subsp. necrophorum and subsp. funduliforme. In cattle, subsp. necrophorum is more prevalent and production of leukotoxin is a major virulence factor. The leukotoxin operon (lktBAC) consists of three genes, lktB, lktA and lktC, of which lktA is the structural toxin gene. The subspecies identity of human F. necrophorum is less certain and it is not known whether human strains possess the leukotoxin gene or leukotoxin activity. Therefore, the objective of this study was to identify the subspecies status of four human clinical strains of F. necrophorum and determine whether they have the leukotoxin gene or leukotoxin activity. Phenotypic and genotypic characteristics suggested that the four strains belonged to subsp. funduliforme, which was confirmed by sequencing the 16S rRNA gene. Analysis of the four strains by PCR revealed the presence of the leukotoxin operon. Partial DNA sequencing identified one human strain with full-length lktA, whereas the others exhibited considerable heterogeneity in size. All strains had a leukotoxin operon promoter-containing intergenic region similar to that of bovine subsp. funduliforme strains, which was confirmed by DNA sequencing and Southern blotting. Despite variations in the lktA gene, all strains secreted leukotoxin as demonstrated by Western blotting. Flow cytometry assays revealed that the leukotoxin was toxic to human white blood cells. In conclusion, the human strains examined contained a leukotoxin gene whose gene product was biologically active. The importance of leukotoxin as a virulence factor in human fusobacterial infections needs further evaluation.
The regulation of nicotinamide adenine dinucleotide biosynthesis by Nampt/PBEF/visfatin in mammals (2007)

We propose that the presumed multiple effects of Nampt/PBEF/visfatin may be entirely explained by its role as an intra and extracellular NAD biosynthetic enzyme. We also propose a new model of Namp/PBEF/visfatin-mediated systemic NAD biosynthesis and its possible physiological significance. Our model provides an important insight into developing preventive/therapeutic interventions for metabolic complications, such as obesity and diabetes.
Active control of fan noise from a turbofan engine (1994)

A three-channel active control system is applied to an operational turbofan engine to reduce tonal noise produced by both the fan and the high-pressure compressor. The control approach is the feedforward filtered-x least-mean-square algorithm implemented on a digital signal processing board. Reference transducers mounted on the engine case provide blade passing and harmonics frequency information to the controller. Error information is provided by large area microphones placed in the acoustic far field. To minimize the error signal, the controller actuates loudspeakers mounted on the inlet to produce destructive interference. The sound pressure level of the fundamental tone of the fan was reduced using the three-channel controller by up to 16 dB over a +/- 30-deg angle about the engine axis. A single-channel controller could produce reduction over a +/- 15-deg angle. The experimental results show the control to be robust. Outside of the areas contolled, the levels of the tone actually increased due to the generation of radial modes by the control sources. Simultaneous control of two tones is achieved with parallel controllers. The fundamental and the first harmonic tones of the fan were controlled simultaneously with reductions of 12 and 5 dBA, respectively, measured on the engine axis. Simultaneous control was also demonstrated for the fan fundamental and the high-pressure compressor fundamental tones.
A new pulse resonant MOSFET gate driver with efficient energy recovery (2006)

To reduce gate driving losses caused by high switching frequency operation, a new pulse resonant gate driver is presented in this paper. The rise/fall time is online controllable, which can be adjusted by the phase angle between driving switches and auxiliary switches (phase shift control), especially suitable for the applications with digital control. To operate more efficiently, an initialized resonant current is generated before turning on/off, which could effectively reduce RMS resonant current with the same rise/fall time. Theoretical and simulation results prove operation and driving loss saving efficiency of the proposed resonant gate drive circuit.
Selective complexometric determination of glyphosate and related compounds (1993)

Abstract A complexometric method has been developed for the selective determination of glyphosate and related compounds. The method is based upon the different pH‐dependence of tridentate and tetradentate ligand‐metal complex stabilities. Glyphosate, the tridentate ligand forms a stable copper complex at pH ≥ 8 only, whereas N ‐carboxymethyl‐ N ‐phosphonomethylglycine and N,N ‐bis(phosphonomethyl)glycine, the tetradentate ligands, form sufficiently stable bismuth complexes even at low pH. The method, therefore, consists of aliquot titrations in basic and acidic media, using metal ion titrant solutions. The first aliquot containing N ‐(phosphonomethyl)glycine plus N ‐carboxymethyl‐ N ‐phosphonomethylglycine or N,N ,‐bis(phosphonomethyl)glycine is titrated with bismuth volumetric solution at pH 1.8–2–5 in the presence of methylthymol blue indicator. Quantities of the tetradentate ligands can be calculated from the bismuth consumption. The second aliquot is titrated with copper volumetric solution at pH 8–10, in the presence of murexide indicator. The content of glyphosate can be calculated from the difference between the copper and bismuth consumptions. Efficacy of the method is verified by analyses of standard mixtures and industrial samples.
Gepirone in the Treatment of Major Depression (1990)

Gepirone is a serotonin (5-hydroxytryptamine, 5-HT) type1A receptor agonist and a pharmacologic analogue of buspirone. Two double-blind, placebo-controlled studies show the efficacy of gepirone in the treatment of major depression. Study 1 demonstrates gepirone's superiority over placebo in an 8-week acute treatment of patients with major depression, including the melancholic subtype. Gepirone's antidepressant dose range is tentatively established at 5-30 mg/day. Study 2 reveals the benefit of gepirone compared with placebo in 4-week continuation therapy of patients with major depression who initially responded to 6 weeks of open therapy with gepirone. Analysis of Hamilton Rating Scale for Depression item scores show gepirone especially improves scores on items of core depression.
A balloon-borne measurement of high latitude atmospheric neutrons using a licaf neutron detector (2013)

PoGOLino is a scintillator-based neutron detector. Its main purpose is to provide data on the neutron flux in the upper stratosphere at high latitudes at thermal and non-thermal energies for the PoGOLite instrument. PoGOLite is a balloon borne hard X-ray polarimeter for which the main source of background stems from high energy neutrons. No measurements of the neutron environment for the planned flight latitude and altitude exist. Furthermore this neutron environment changes with altitude, latitude and solar activity, three variables that will vary throughout the PoGOLite flight. PoGOLino was developed to study the neutron environment and the influences from these three variables upon it. PoGOLino consists of two Europium doped Lithium Calcium Aluminium Fluoride (Eu:LiCAF) scintillators, each of which is sandwiched between 2 Bismuth Germanium Oxide (BGO) scintillating crystals, which serve to veto signals produced by gamma-rays and charged particles. This allows the neutron flux to be measured even in high radiation environments. Measurements of neutrons in two separate energy bands are achieved by placing one LiCAF detector inside a moderating polyethylene shield while the second detector remains unshielded. The PoGOLino instrument was launched on March 20th 2013 from the Esrange Space Center in Northern Sweden to an altitude of 30.9 km. A description of the detector design and read-out system is presented. A detailed set of simulations of the atmospheric neutron environment performed using both PLANETOCOSMICS and Geant4 will also be described. The comparison of the neutron flux measured during flight to predictions based on these simulations will be presented and the consequences for the PoGOLite background will be discussed.
Experimental Investigation of Variable Damping Properties for Viscous Damper Utilizing a Magnetic Responsive Fluid (2005)

A basic experimental study was carried out to investigate the parameters in a vibration equation of a viscous damper utilizing a magnetic responsive fluid in order to design a semi-active viscous damper. We used the magnetic piston which has made with alternating layers of magnet and non-magnetic material disks for particles of the fluids not to sediment. To clarify the damping effect, we measured a frequency response and a relationship between inertia force and displacement. The results of the dynamic characteristic of the damper can be explained by the cluster of particles aggregated like a chain in the fluid. We also clarify that MCF is suitable for the damper. The damping effect is strongly influenced by an apparent density around the piston of the damper.
Extending Timescales and Narrowing Linewidths in NMR (2011)

Among the different fields of research in nuclear magnetic resonance (NMR) which are currently investigated in the Laboratory of Biomolecular Magnetic Resonance (LRMB), two subjects that are closely related to each other are presented in this article. On the one hand, we show how to populate long-lived states (LLS) that have long lifetimes T(LLS) which allow one to go beyond the usual limits imposed by the longitudinal relaxation time T1. This makes it possible to extend NMR experiments to longer time-scales. As an application, we demonstrate the extension of the timescale of diffusion measurements by NMR spectroscopy. On the other hand, we review our work on long-lived coherences (LLC), a particular type of coherence between two spin states that oscillates with the frequency of the scalar coupling constant J(IS) and decays with a time constant T(LLC). Again, this time constant T(LLC) can be much longer than the transverse relaxation time T2. By extending the coherence lifetimes, we can narrow the linewidths to an unprecedented extent. J-couplings and residual dipolar couplings (RDCs) in weakly-oriented phases can be measured with the highest precision.
A new reconstruction algorithm for Radon data (2006)

A new reconstruction algorithm for Radon data is introduced. We call the new algorithm OPED as it is based on Orthogonal Polynomial Expansion on the Disk. OPED is fundamentally different from the filtered back projection (FBP) method. It allows one to use fan beam geometry directly without any additional procedures such as interpolation or rebinning. It reconstructs high degree polynomials exactly and works for smooth functions without the assumption that functions are band- limited. Our initial tests indicate that the algorithm is stable, provides high resolution images, and has a small global error. Working with the geometry specified by the algorithm and a new mask, OPED could also lead to a reconstruction method that works with reduced x-ray dose (see the paper by Tischenko et al in these proceedings).
Cannabinoid CB2 Receptor Potentiates Obesity-Associated Inflammation, Insulin Resistance and Hepatic Steatosis (2009)

These data unravel a previously unrecognized contribution of CB2 receptors to obesity-associated inflammation, insulin resistance and non-alcoholic fatty liver disease, and suggest that CB2 receptor antagonists may open a new therapeutic approach for the management of obesity-associated metabolic disorders.
New design for compact liquid-gas partial separation: Downhole and surface installations for artificial lift applications (1995)

In many applications, complete separation of liquid and gas is not required, and it is sufficient to remove only a portion of the gas. One example is separation of gas from one well for raw gas lift of other wells. This can be done either on the surface or downhole. The auger separator is a new device that was initially developed to fit inside production tubing, and so is an ultra-compact separator design. It differs from other separators in that it requires no complex controls or power to operate, and, for most applications, has a diameter of only a few inches. The multiphase fluid enters an auger section where the pitch of the stationary auger blade causes the flow to rotate. Centrifugal forces created by this rotation cause the liquid to flow along the outer wall with the gas flowing in the center. A portion of the gas is ported through a crossover to the annulus in downhole applications or to a gas line in surface applications. The downhole separator is placed by wireline in the tubing. The surface version is placed as a spool piece in the production line. Equations were developed to predict performance and were incorporated into a spreadsheet for ease of design. Laboratory tests were performed to validate the predictions and to explore various design details. Following successful lab testing, the prototype separator, which was 3-1/2 inches in diameter, was placed downhole inside 4-1/2 tubing. The separated gas was ported to the annulus through a gas lift mandrel. This test showed that the equipment could be placed in the well with wireline, that downhole separation was feasible, and that the theoretical pressure drop calculations were accurate. Following this test, an 8-inch diameter auger separator was designed for separation of gas on the surface, as a full scale pilot test. This was placed in the production line from a well, and the separated gas was used to provide raw lift gas for other wells on the drillsite. These two field tests showed that partial separation was feasible with this device, and that the equations used for design were reasonable. The surface auger separator was left in place after the test, and continues to provide lift gas. This paper presents the concept, design equations, lab test results, and field test results of both a downhole and surface application. Other potential uses are also discussed.
Generation of an Adenovirus-Parvovirus Chimera with Enhanced Oncolytic Potential (2012)

In this study, our goal was to generate a chimeric adenovirus-parvovirus (Ad-PV) vector that combines the high-titer and efficient gene transfer of adenovirus with the anticancer potential of rodent parvovirus. To this end, the entire oncolytic PV genome was inserted into a replication-defective E1- and E3-deleted Ad5 vector genome. As we found that parvoviral NS expression inhibited Ad-PV chimera production, we engineered the parvoviral P4 early promoter, which governs NS expression, by inserting into its sequence tetracycline operator elements. As a result of these modifications, P4-driven expression was blocked in the packaging T-REx-293 cells, which constitutively express the tetracycline repressor, allowing high-yield chimera production. The chimera effectively delivered the PV genome into cancer cells, from which fully infectious replication-competent parvovirus particles were generated. Remarkably, the Ad-PV chimera exerted stronger cytotoxic activities against various cancer cell lines, compared with the PV and Ad parental viruses, while being still innocuous to a panel of tested healthy primary human cells. This Ad-PV chimera represents a novel versatile anticancer agent which can be subjected to further genetic manipulations in order to reinforce its enhanced oncolytic capacity through arming with transgenes or retargeting into tumor cells.
Functionalized congeners of adenosine: preparation of analogs with high affinity for A1-adenosine receptors (1985)

A series of functionalized congeners of adenosine based on N6-phenyladenosine, a potent A1-adenosine receptor against, was synthesized. Derivatives of the various congeners should be useful as receptor and histochemical probes and for the preparation of radioligands and affinity columns or as targeted drugs. N6-[4-(Carboxymethyl)phenyl]adenosine served as the starting point for synthesis of the methyl ester, the methyl amide, the ethyl glycinate, and various substituted anilides. One of the latter, N6-[4-[[[4-(carbomethoxymethyl)anilino]carbonyl]methyl]phenyl] adenosine, served as the starting point for the synthesis of another series of congeners including the methyl amide, the hydrazide, and the aminoethyl amide. The terminal amino function of the last congener was acylated to provide further analogues. The various congeners were potent competitive antagonists of binding of N6-[3H]cyclohexyladenosine to A1-adenosine receptors in rat cerebral cortical membranes. The affinity of the congener for the A1 receptor was highly dependent on the nature of the spacer group and the terminal moiety with Ki values ranging 1-100 nM. A biotinylated analogue had a Ki value of 11 nM. A conjugate derived from the Bolton-Hunter reagent had a Ki value of 4.5 nM. The most potent congener contained a terminal [(aminoethyl)amino]carbonyl function and had a Ki value of less than 1 nM.
A novel multipurpose tree and path matching algorithm with application to airway trees (2006)

Tree matching methods have numerous applications in medical imaging, including registration, anatomical labeling, segmentation, and navigation of structures such as vessels and airway trees. Typical methods for tree matching rely on conventional graph matching techniques and therefore suffer potential limitations such as sensitivity to the accuracy of the extracted tree structures, as well as dependence on the initial alignment. We present a novel path-based tree matching framework independent of graph matching. It is based on a point-by-point feature comparison of complete paths rather than branch points, and consequently is relatively unaffected by spurious airways and/or missing branches. A matching matrix is used to enforce one-to-one matching. Moreover our method can reliably match irregular tree structures, resulting from imperfect segmentation and centerline extraction. Also reflecting the nature of these features, our method does not require a precise alignment or registration of tree structures. To test our method we used two thoracic CT scans from each of ten patients, with a median inter-scan interval of 3 months (range 0.5 to 10 months). The bronchial tree structure was automatically extracted from each scan and a ground truth of matching paths was established between each pair of tree structures. Overall 87% of 702 airway paths (average 35.1 per patient matched both ways) were correctly matched using this technique. Based on this success we also present preliminary results of airway-to-artery matching using our proposed methodology.
Low Band Gap Benzimidazole COF Supported Ni<sub>3</sub>N as Highly Active OER Catalyst (2016)

Covalent organic frameworks (COFs) have structures and morphologies closely resembling graphenes, whose modular construction permits atomic‐level manipulations. This, combined with their porous structure, makes them excellent catalyst supports. Here, the high electrocatalytic activity of a composite, formed by supporting Ni 3 N nanoparticles on a benzimidazole COF, for oxygen evolution reaction is shown. The composite oxidizes alkaline water with a near‐record low overpotential of 230 mV @ 10 mA cm −2 ( η 10 ). This high activity is attributed to the ability of the COF to confine the Ni 3 N nanoparticles to size regimes otherwise difficult to obtain and to its low band gap character (1.49 eV) arising from the synergy between the conducting Ni 3 N nanoparticles and the π‐conjugated COF. The COF itself, as a metal‐free self‐standing framework, has an oxygen evolution reaction activity with η 10 of 400 mV. The periodic structure of the COF makes it serve as a matrix to disperse the catalytically active Ni 3 N nanoparticles favoring their high accessibility and thereby good charge‐transport within the composite. This is evident from the amount of O 2 evolved (230 mmol h −1 g −1 ), which, to the best of our knowledge, is the highest reported. The work reveals the emergence of COF as supports for electrocatalysts.
Cis D4Z4 repeat duplications associated with facioscapulohumeral muscular dystrophy type 2 (2018)

Facioscapulohumeral muscular dystrophy, known in genetic forms FSHD1 and FSHD2, is associated with D4Z4 repeat array chromatin relaxation and somatic derepression of DUX4 located in D4Z4. A complete copy of DUX4 is present on 4qA chromosomes, but not on the D4Z4-like repeats of chromosomes 4qB or 10. Normally, the D4Z4 repeat varies between 8 and 100 units, while in FSHD1 it is only 1-10 units. In the rare genetic form FSHD2, a combination of a 4qA allele with a D4Z4 repeat size of 8-20 units and heterozygous pathogenic variants in the chromatin modifier SMCHD1 causes DUX4 derepression and disease. In this study, we identified 11/79 (14%) FSHD2 patients with unusually large 4qA alleles of 21-70 D4Z4 units. By a combination of Southern blotting and molecular combing, we show that 8/11 (73%) of these unusually large 4qA alleles represent duplication alleles in which the long D4Z4 repeat arrays are followed by a small FSHD-sized D4Z4 repeat array duplication. We also show that these duplication alleles are associated with DUX4 expression. This duplication allele frequency is significantly higher than in controls (2.9%), FSHD1 patients (1.4%) and in FSHD2 patients with typical 4qA alleles of 8-20 D4Z4 units (1.5%). Segregation analysis shows that, similar to typical 8-20 units FSHD2 alleles, duplication alleles only cause FSHD in combination with a pathogenic variant in SMCHD1. We conclude that cis duplications of D4Z4 repeats explain DUX4 expression and disease presentation in FSHD2 families with unusual long D4Z4 repeats on 4qA chromosomes.
Effect of alloying elements on room temperature tensile ductility in magnesium alloys (2016)

The impact of alloying elements on the room temperature tensile behaviour was investigated for a wide range of strain rates using eight types of extruded Mg-0.3 at.% X (X = Ag, Al, Li, Mn, Pb, Sn, Y and Zn) binary alloys with an average grain size of 2–3 μm. The solid solution alloying element affected not only tensile plasticity but also rate-controlling mechanism for these fine-grained magnesium alloys. Most of the alloys exhibited an elongation-to-failure of 20–50% , while the alloys with a high m-value exhibited large tensile plasticity, such as an elongation-to-failure of 140% in a strain rate of 1 × 10−5 s−1 for the Mg–Mn alloy. This elongation-to-failure is more than two times larger than that for pure magnesium. This is due to the major contribution of grain boundary sliding (GBS) on the deformation. Microstructural observations reveal that grain boundary segregation, which is likely to affect gain boundary energy, plays a role in the prevention or enhancement of GBS. The present results are clearly expected to open doors to the development of magnesium alloys with good secondary formability at room temperature through the control of alloying elements.