gebhart/patrepeval huggingface dataset:
OXYRASE<sup>TM</sup> ENZYME AND MOTILITY ENRICHMENT FUNG‐YU TUBE FOR RAPID DETECTION OF <i>LISTERIA MONOCYTOGENES</i> AND <i>LISTERIA</i> SPECIES<sup>1</sup> (1990)

ABSTRACT A rapid and simple method using a U‐shaped glass apparatus (Fung‐Yu tube) for early determination of the presence of Listeria monocytogenes and Listeria species in mixed cultures and inoculated meat samples has been developed. This system utilizes unique biochemical and physical properties of Listeria for selective enrichment. Fraser broth was used as a selective enrichment broth especially for observation of esculin hydrolysis (blackening of broth), and semisolid Modified Oxford agar was used for selective detection of motility of Listeria. When Fung‐Yu tubes containing 0.1 unit/mL of Oxyrase TM ( membrane fractions of Escherichia coli) were inoculated with L. monocytogenes, an enhanced early growth of L. monocytogenes occurred. A presumptive positive result for low numbers of L. monocytogenes ( 1–100 CFU/g) in the presence of large numbers of competitive microflora in pre‐enriched (24 h) ground beef samples using the Fung‐Yu tube method with the aid of Oxyrase TM was obtainable within 10 h. Using this system, isolation of Listeria in the presence of mixed bacterial flora (44 species), such as Bacillus, Escherichia, Klebsiella, Proteus, Salmonella, Shigella, Staphylococcus, and Streptococcus, and in inoculated ground beef was successful in 24–48 h. The Fung‐Yu tube procedure is a highly sensitive, selective, and easy‐to‐use method to separate and isolate L. monocytogenes and other Listeria spp. from other contaminating microorganisms in meats .
[O1‐04‐05]: Subgroups of Alzheimer disease based on cerebrospinal fluid levels of molecular markers (2005)

Alzheimer disease, the most common cause of dementia, is both polyetiological and heterogeneous, and its clinical diagnosis remains probable, to date. We postulated that more than one disease mechanism might be involved in producing the Alzheimer histopathology and that various subgroups of the disease might be identified based on the CSF levels of the proteins associated with senile (neuritic) plaques and neurofibrillary tangles, the two histopathological hallmarks of the disease. We immunoassayed the levels of tau, ubiquitin and Aβ1–42 in retrospectively collected CSFs of 468 patients clinically diagnosed as Alzheimer disease (353 CSFs) and as non-Alzheimer neurological or non-neurological cases (115 CSFs), and based on the levels of these molecular markers, all subjects were subjected to latent profile analysis to determine the assignment of each subject to a particular cluster. We found that Alzheimer disease subdivided into at least five subgroups based on the CSF levels of Aβ1–42, tau and ubiquitin, and that each subgroup presented a different clinical profile. Alzheimer disease appears to be a family of closely related disorders which subdivides into at least five subgroups. Each of these subgroups, which can be identified by the CSF analysis, might benefit differently from different therapeutic drugs.
Probiotic<i>Lactobacillus</i>dose required to restore and maintain a normal vaginal flora (2001)

Forty-two healthy women were randomized to receive one of three encapsulated Lactobacillus rhamnosus GR-1 plus Lactobacillus fermentum RC-14 probiotic dosage regimens or L. rhamnosus GG by mouth each day for 28 days. However, the vaginal flora, assessed by Nugent scoring, was only normal in 40% of the cases, and 14 patients had asymptomatic bacterial vaginosis. Treatment with L. rhamnosus GR-1/L. fermentum RC-14 once and twice daily correlated with a healthy vaginal flora in up to 90% of patients, and 7/11 patients with bacterial vaginosis converted to normal or intermediate scores within 1 month. Ingestion of L. rhamnosus GG failed to have an effect. This study confirms the potential efficacy of orally administered lactobacilli as a non-chemotherapeutic means to restore and maintain a normal urogenital flora, and shows that over 10(8) viable organisms per day is the required dose.
Layered Nanocomposites from Gold Nanoparticles for Neural Prosthetic Devices (2012)

Treatments of neurological diseases, diagnostics of brain malfunctions, and the realization of brain-computer interfaces require ultrasmall electrodes that are "invisible" to resident immune cells. Functional electrodes smaller than 50 μm are impossible to produce with traditional materials due to high interfacial impedance at the characteristic frequency of neural activity and insufficient charge storage capacity. The problem can be resolved by using gold nanoparticle nanocomposites. Careful comparison indicates that layer-by-layer assembled films from Au NPs provide more than 3-fold improvement in interfacial impedance and 1 order of magnitude increase in charge storage capacity. Prototypes of microelectrodes could be made using traditional photolithography. Integration of unique nanocomposite materials with microfabrication techniques opens the door for practical realization of the ultrasmall implantable electrodes. Further improvement of electrical properties is expected when using special shapes of gold nanoparticles.
A Reduced Order Model for Transient Analysis of Bladed Disk Forced Response (2005)

A method for predicting the vibratory response of bladed disks under high engine acceleration rates is developed. The method is based on the Fundamental Mistuning Model, an existing reduced order model for predicting the steady-state vibratory response. In addition, a criterion is developed for a critical engine acceleration rate, above which transient effects play a large role in the response. It is shown that military engines operate at acceleration rates above this critical value and therefore transient effects are important in practice.
Formation and Activity of Template-Assembled Receptor Signaling Complexes (2007)

Problems in membrane biology require methods to recreate the interactions between receptors and cytoplasmic signaling proteins at the membrane surface. Here, unilamellar vesicles composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine and a nickel-chelating lipid were used as templates to direct the assembly of proteins from the Escherichia coli chemotaxis signaling pathway. The bacterial chemoreceptors are known to form clusters, which promote the binding of the adaptor protein (CheW) and the kinase (CheA). When CheA was incubated with vesicles, CheW, and a histidine-tagged cytoplasmic domain fragment of the aspartate chemoreceptor (CF), the kinase activity was stimulated approximately 300-fold. Activity and pull-down assays were used with dynamic light scattering and electron microscopy to characterize the protein-vesicle compositions that were correlated with the high levels of activity, which demonstrated that CF-CheW-CheA complexes on the vesicle surface were the active entities. Assembly and stimulation occurred with vesicles of different sizes and CFs in different extents of glutamine substitution (in place of glutamate) at physiologically relevant sites. An exception was the combination of sonicated vesicles with the unsubstituted CF, which displayed lower CheA activity. The lower activity was attributed to the high curvature of the sonicated vesicles and a weaker tendency of the unsubstituted CF to self-assemble. Electron micrographs of the vesicle-protein assemblies revealed that protein binding induced pronounced changes in vesicle shape, which was consistent with the introduction of positive curvature in the outer leaflet of the bilayer. Overall, vesicle-mediated template-directed assembly is shown to be an effective way to form functional complexes of membrane-associated proteins and suggests that significant changes in membrane shape can be involved in the process of transmembrane signaling.
GeSn Nanobeam Light-Emitting Diode as a GHz-Modulated Light Source (2017)

Designs and theoretical analysis are presented for a room temperature resonant-cavity-enhanced GeSn LED whose emission peaks at the 2 <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><tex-math notation="LaTeX">$\mu$</tex-math></inline-formula> m wavelength. The Ge/GeSn/Ge PIN hetero-diode of length 1 <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><tex-math notation="LaTeX">$\mu$</tex-math></inline-formula> m is embedded in a rib-type Ge-on-Si nanobeam having either 24 or 36 air holes. The maximum LED modulation bandwidth <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><tex-math notation="LaTeX">$f_{3\text{dB}}$</tex-math></inline-formula> is proportional to the Purcell factor and is inversely proportional to <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><tex-math notation="LaTeX">$\tau _{s\,p0}$</tex-math> </inline-formula> the GeSn bulk spontaneous emission lifetime. For an emission linewidth of 200 nm and <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$\tau _{s\,p0}$</tex-math></inline-formula> of 10 ns, an <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$f_{3\text{dB}}$</tex-math></inline-formula> of 1.6 GHz is predicted.
A 75-ch SQUID Biomagnetometer System for Human Cervical Spinal Cord Evoked Field (2007)

A 75-ch SQUID biomagnetometer system for the measurement of the cervical spinal cord evoked magnetic field (SCEF) was developed for the purpose of the noninvasive functional diagnosis of the spinal cord. The sensor array has 25 SQUID vector sensors arranged along the cylindrical surface to fit to the shape of the subject's neck. The magnetic fields, not only in the direction radial to the subject's body surface but also in the tangential direction, are observed in the area of 80 mm times 90 mm at one time. The dewar has a unique shape with a cylindrical main body and a protrusion from its side surface. The sensor array is installed in the protruded part. This design is optimized to detect magnetic signals at the back of the neck of the subject sitting in a reclining position. We applied the developed SQUID system to the cervical SCEF measurement of normal subjects who were given electric pulse stimulation to their median nerves at the wrists. The evoked magnetic signals were successfully detected at the cervixes of all subjects. A characteristic pattern of transition of the SCEF distribution was observed as a reproducible result and the signal components propagating along the spinal cord were found in the time varying SCEF distribution. We expect that the investigation of the propagating signal components would help to establish a noninvasive functional diagnosis of the spinal cord.
A Compact Single-Layer Four-Port Orthogonally Polarized Yagi-Like MIMO Antenna System (2020)

A wideband compact Yagi-like directional multiple-input- multiple-output (MIMO) antenna system is presented based on a slot loop excitation. Four orthogonally polarized beams are generated by orthogonally feeding the slot loop elements using microstrip lines. The proposed design is based on a single-layer geometry with an overall size of 35 × 90 × 0.76 mm3, which is suitable for compact wireless handheld devices. The conventional vertically polarized omnidirectional beams from each slot loop element are made directional and horizontally polarized by using two passive complementary slot reflector (CSR) elements. The antenna system has a wide measured bandwidth of 620 MHz (3.26-3.88 GHz) targeting the 3.6 GHz WiMAX band. It has a maximum realized gain of 4 dBi and front to back ratio (FBR) of more than 7 dB within the band of operation. The proposed antenna system shows good MIMO performance with a minimum measured isolation of 10 dB between the adjacent ports and more than 20 dB between other ports. The orthogonal beams of the antenna system help in achieving low envelope correlation coefficient (ECC) values of less than 0.1. The measured total efficiency of the antenna system is more than 75% for all the ports of the antenna system.
Identification and Functional Characterization of Brainstem Cannabinoid CB<sub>2</sub>Receptors (2005)

The presence and function of CB2 receptors in central nervous system (CNS) neurons are controversial. We report the expression of CB2 receptor messenger RNA and protein localization on brainstem neurons. These functional CB2 receptors in the brainstem were activated by a CB2 receptor agonist, 2-arachidonoylglycerol, and by elevated endogenous levels of endocannabinoids, which also act at CB1 receptors. CB2 receptors represent an alternative site of action of endocannabinoids that opens the possibility of nonpsychotropic therapeutic interventions using enhanced endocannabinoid levels in localized brain areas.
Control of video processing algorithms based on measured perceptual quality characteristics (2012)

Most consumer electronic devices include a suite of video post-processing algorithms that process the output of the video decoder to drive the video display. In this paper, we propose the use of non-reference video quality metrics to improve the Quality of Experience (QoE) of the user on-the-fly by controlling the parameters of the video post-processing pipeline. We utilize non-reference and real-time measurements of perceptual contrast, sharpness and noise to control denoising and contrast enhancement algorithms in the video post-processing pipeline. We propose methods to ensure consistency of the post-processing across the different frames of the video to avoid creation of flickering artifacts due to variable post-processing. We show that video quality can be improved by identifying the specific quality characteristics that are altered by a video post-processing method and using measurements of these characteristics to improve overall user QoE.
Trace norm regularization and faster inference for embedded speech recognition RNNs (2017)

We propose and evaluate new techniques for compressing and speeding up dense matrix multiplications as found in the fully connected and recurrent layers of neural networks for embedded large vocabulary continuous speech recognition (LVCSR). For compression, we introduce and study a trace norm regularization technique for training low rank factored versions of matrix multiplications. Compared to standard low rank training, we show that our method leads to good accuracy versus number of parameter trade-offs and can be used to speed up training of large models. For speedup, we enable faster inference on ARM processors through new open sourced kernels optimized for small batch sizes, resulting in 3x to 7x speed ups over the widely used gemmlowp library. Beyond LVCSR, we expect our techniques and kernels to be more generally applicable to embedded neural networks with large fully connected or recurrent layers.
Insights from Zebrafish and Mouse Models on the Activity and Safety of Ar-Turmerone as a Potential Drug Candidate for the Treatment of Epilepsy (2013)

In a previous study, we uncovered the anticonvulsant properties of turmeric oil and its sesquiterpenoids (ar-turmerone, α-, β-turmerone and α-atlantone) in both zebrafish and mouse models of chemically-induced seizures using pentylenetetrazole (PTZ). In this follow-up study, we aimed at evaluating the anticonvulsant activity of ar-turmerone further. A more in-depth anticonvulsant evaluation of ar-turmerone was therefore carried out in the i.v. PTZ and 6-Hz mouse models. The potential toxic effects of ar-turmerone were evaluated using the beam walking test to assess mouse motor function and balance. In addition, determination of the concentration-time profile of ar-turmerone was carried out for a more extended evaluation of its bioavailability in the mouse brain. Ar-turmerone displayed anticonvulsant properties in both acute seizure models in mice and modulated the expression patterns of two seizure-related genes (c-fos and brain-derived neurotrophic factor [bdnf]) in zebrafish. Importantly, no effects on motor function and balance were observed in mice after treatment with ar-turmerone even after administering a dose 500-fold higher than the effective dose in the 6-Hz model. In addition, quantification of its concentration in mouse brains revealed rapid absorption after i.p. administration, capacity to cross the BBB and long-term brain residence. Hence, our results provide additional information on the anticonvulsant properties of ar-turmerone and support further evaluation towards elucidating its mechanism of action, bioavailability, toxicity and potential clinical application.
CMOS-Compatible Protonic Programmable Resistor Based on Phosphosilicate Glass Electrolyte for Analog Deep Learning (2021)

Ion intercalation based programmable resistors have emerged as a potential next-generation technology for analog deep-learning applications. Proton, being the smallest ion, is a very promising candidate to enable devices with high modulation speed, low energy consumption, and enhanced endurance. In this work, we report on the first back-end CMOS-compatible nonvolatile protonic programmable resistor enabled by the integration of phosphosilicate glass (PSG) as the proton solid electrolyte layer. PSG is an outstanding solid electrolyte material that displays both excellent protonic conduction and electronic insulation characteristics. Moreover, it is a well-known material within conventional Si fabrication, which enables precise deposition control and scalability. Our scaled all-solid-state three-terminal devices show desirable modulation characteristics in terms of symmetry, retention, endurance, and energy efficiency. Protonic programmable resistors based on phosphosilicate glass, therefore, represent promising candidates to realize nanoscale analog crossbar processors for monolithic CMOS integration.
Abstract 2267: Natural product-inspired drug discovery: Chemistry and biology of oridonin analogs with unique scaffolds and enhanced anticancer profiles . (2013)

Abstract ABSTRACT: Oridonin, a complex ent-kaurane diterpenoid isolated from Chinese traditional herb Rabdosia rubescens (Chinese name "Donglingcao"), has demonstrated great potential in the treatment of various human cancers due to its unique, safe and remarkable anticancer pharmacological profile. Nevertheless, the clinical development of oridonin for cancer therapy has been hampered by its relatively moderate potency, limited aqueous solubility and bioavailability. Up to now, the oridonin-based structural modifications and relevant structure-activity relationship remain sparse partially because of the challenging synthesis and structural complexity. To develop novel oridonin derivatives with enhanced anticancer potency and solubility, a sizable compound library of oridonin analogs with unique scaffolds is being established in our laboratories through diversity-orientated synthesis. Our successful synthetic strategies include heterocycle-fused ring A construction, and ring A-based α,β-unsaturated enone formation, as well as other stereoselective and regioselective approaches. A number of novel nitrogen-enriched heterocyclic oridonin derivatives and dienone analogs such as CYD0554, CYD0618, CYD0628, CYD0630, CYD0686, and CYD0692 have been identified with potent anti-proliferation effects against breast, pancreatic, and prostate cancer cells with low micromolar to nanomolar IC50 values. Moreover, some of them also exhibit significantly improved aqueous solubility and enhanced anticancer profiles for the treatment of estrogen receptor (ER)-negative including triple-negative breast cancer in comparison with oridonin. Our success in the chemical design, synthesis and biological characterization of these new molecules opens new avenues to develop promising natural product-like drug candidates for cancer therapy. Key Words: Natural products, Oridonin analogs, Diversity-orientated synthesis, Anticancer agents, Drug discovery Citation Format: Chunyong Ding, Yusong Zhang, Haijun Chen, Zhengduo Yang, Lili Chu, Christopher Wild, Mark A. White, Qiang Shen, Jia Zhou. Natural product-inspired drug discovery: Chemistry and biology of oridonin analogs with unique scaffolds and enhanced anticancer profiles . [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 2267. doi:10.1158/1538-7445.AM2013-2267
Manganese peroxidase from the white‐rot fungus <i>Phanerochaete chrysosporium</i> is enzymatically active and accumulates to high levels in transgenic maize seed (2005)

Manganese peroxidase (MnP) has been implicated in lignin degradation and thus has potential applications in pulp and paper bleaching, enzymatic remediation and the textile industry. Transgenic plants are an emerging protein expression platform that offer many advantages over traditional systems, in particular their potential for large-scale industrial enzyme production. Several plant expression vectors were created to evaluate the accumulation of MnP from the wood-rot fungus Phanerochaete chrysosporium in maize seed. We showed that cell wall targeting yielded full-length MnP, whereas cytoplasmic localization resulted in multiple truncated peroxidase polypeptides as detected by immunoblot analysis. In addition, the use of a seed-preferred promoter dramatically increased the expression levels and reduced the negative effects on plant health. Multiple independent transgenic lines were backcrossed with elite inbred corn lines for several generations with the maintenance of high-level expression, indicating genetic stability of the transgene.
Systolic super summation with reduced hardware (1992)

A systolic super summer is a cellular apparatus for summing floating-point numbers. The apparatus receives floating-point summands, converting them to fixed-point within a sieve-like cellular array. The emerging fixed-point numbers are summed in a pipelined array of long accumulators. An improved design is presented for the summer's sieve. Although the new sieve is structurally simpler and uses less hardware, the throughput per unit area is the same as that for the previously designed sieve. The new sieve's architectural regularity makes it ideal for implementation in VLSI circuit technology.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Anticorrosion properties of CeO2-modified aluminosilicate nanotubes (2013)

Ceria nanoparticles 4–15 nm in size have been obtained on halloysite nanotubes (HNTs) in solution by reacting cerium nitrate and sodium hydroxide. The HNT/CeO2 composites were characterized by transmission electron microscopy, scanning electron microscopy, X-ray diffraction, and infrared spectroscopy. Modified HNTs containing about 5 wt % ceria showed high anticorrosion performance on 2024-T3 air-craft-grade aluminum alloy.
A digital quadrature demodulation system (1996)

Existing digital quadrature demodulation techniques sample the input at either 2B Hz or 4B Hz, select the even samples as the in-phase (I), and interpolate the odd samples to give the quadrature (Q), output. The signal bandwidth is B. We propose a demodulation system to produce I and Q samples at arbitrary sampling rate greater than 2B Hz. The system eliminates the IF downconversion step with a special sampling scheme. The even samples correspond to the I component, while the Q components are the filtered output. The filter can be a lowpass or least squares (LS) filter. The lowpass filter design is based on trade-offs between the filter length and the degree of oversampling. It produces similar results as previous work when the sampling rate is 2B Hz or 4B Hz. Unlike existing methods which assume the input is white, a LS filter, on the other hand, can make use of input signal characteristics to achieve a better result. The higher the correlation in the input the larger the improvement. The cost for LS filtering is a coefficient update step if the input is time varying. A scheme to cancel dc offset from analog to digital (A/D) converters is also given.