gebhart/patrepeval huggingface dataset:
Purification of a Large Protein Using Ion-Exchange Membranes (2002)

Anion-exchange membranes were evaluated for the capture of a small protein (α-lactalbumin, 3.5-nm diameter) and a large protein (thyroglobulin, 20-nm diameter). The static binding capacity equaled the dynamic binding capacity and increased with increasing protein size. This result was in agreement with calculations based on monolayer coverage on the membrane surface and an absence of mass-transfer limitations. In contrast, for anion-exchange beads, the static capacity was the same for both proteins, and the dynamic capacity decreased strikingly with increasing protein size. These observations were attributed to very slow intrapore diffusion for large proteins in the beads, resulting in surface binding only. This work has important applications in the selection of chromatography media for the purification of viruses and plasmid DNA. Specifically, membranes with a high capacity for large biomolecules (20−300 nm) and a low capacity for small host-cell proteins and endotoxin contaminants are preferable to beads for the purification of such biomolecules.
Risk of Dental Disease Non-Battle Injuries and Severity of Dental Disease in Deployed U.S. Army Personnel (2015)

Dental Disease and Non-Battle Injuries (D-DNBI) continue to be a problem among U.S. Army active duty (AD), U.S. Army National Guard (ARNG), and U.S. Army Reserve (USAR) deployed soldiers to Operation Iraqi Freedom/Operation New Dawn in Iraq and Operation Enduring Freedom in Afghanistan. A previous study reported the annual rates to be 136 D-DNBI per 1,000 personnel for AD, 152 for ARNG, and 184 for USAR. The objectives of this study were to describe D-DNBI incidence and to determine risk factors for dental encounters and high severity diagnoses for deployed soldiers. The 78 diagnoses were classified into three categories based on severity. Poisson regression was used to compare D-DNBI rates and logistic regression was used to analyze the risk of high severity D-DNBI. In both campaigns, Reserve had a higher risk of D-DNBI than active duty. For Afghanistan, ARNG and USAR demonstrated over 50% increased risk of D-DNBI compared to AD. In Iraq, USAR had a 17% increased risk over AD. Females had a higher risk of D-DNBI (>50%) compared to males in both campaigns. High severity D-DNBI made up 2.77% of all diagnoses. Within Afghanistan, there was a 4.6% increased risk of high severity D-DNBI for each additional deployment month.
SWIPT in MISO Multicasting Systems (2014)

This letter considers simultaneous wireless information and power transfer (SWIPT) in multiple-input single-output (MISO) multicasting systems where each receiver is equipped with a power splitting device and can receive both information and energy from the base station (BS) continuously at the same time. We investigate the joint multicast transmit beamforming and receive power splitting problem for minimizing the transmit power of the BS subject to signal-to-noise ratio (SNR) and energy harvesting constraints at each receiver. Both scenarios of perfect and imperfect channel state information (CSI) at the BS are studied. Due to non convexity of the problems, we use semidefinite relaxation (SDR) technique to solve the problems. Interestingly, we show that the SDR is in fact tight in certain scenarios.
Fully Stereocontrolled Total Syntheses of (−)‐Cylindricine C and (−)‐2‐Epicylindricine C: A Departure in Sulfonamide Chemistry (2004)

A common intermediate was used in the total syntheses of (−)-cylindricine C and (−)-2-epicylindricine C (see picture) in a process that involves the initial oxidative spirocyclization of a phenolic sulfonamide. The remainder of the molecules is constructed through interesting sulfonamide chemistry. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2004/z460178_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Composition and thermal properties of Rhea oil and its fractions (2005)

The rhea (Rhea americana) is a large running bird of the ratite family, native to South America. Oil extracted from rhea fat tissue is used in cosmetic manufacture. Here, the thermal behaviour and the fatty acid and triacylglycerol composition of Uruguayan rhea oil are studied. The results are compared with those obtained from two commercial samples of emu oil. The fatty acid profiles of emu and rhea oils are similar. Small variations are reflected in the non-identical thermal behaviour of the oils. The solid content of both oils is fairly similar at room temperature. Thus, emu oil and rhea oil may replace one another in certain formulations, without resulting in important changes in physicochemical behaviour. The semisolid rhea oil was fractionated in two successive stages: an olein was obtained at 15 °C, which was refractionated at 10 °C. The thermogram of the olein obtained by cooling at 15 °C does not have the peak found at 34 °C in the thermogram of the original oil and is a softer product than the original oil. A further stage of fractionation of this olein produced a new liquid phase of slightly different thermal behaviour from that of the original olein. This product has a solid fat index around 7% at 15 °C and has melted completely at 20 °C. This second olein has more appropriate physical characteristics than those of the olein obtained from the first fractionation when used in liquid cosmetic formulations.
Exploiting Blockchain Data to Detect Smart Ponzi Schemes on Ethereum (2019)

Blockchain technology becomes increasingly popular. It also attracts scams, for example, a Ponzi scheme, a classic fraud, has been found making a notable amount of money on Blockchain, which has a very negative impact. To help to deal with this issue and to provide reusable research data sets for future research, this paper collects real-world samples and proposes an approach to detect Ponzi schemes implemented as smart contracts (i.e., smart Ponzi schemes) on the blockchain. First, 200 smart Ponzi schemes are obtained by manually checking more than 3,000 open source smart contracts on the Ethereum platform. Then, two kinds of features are extracted from the transaction history and operation codes of the smart contracts. Finally, a classification model is presented to detect smart Ponzi schemes. The extensive experiments show that the proposed model performs better than many traditional classification models and can achieve high accuracy for practical use. By using the proposed approach, we estimate that there are more than 500 smart Ponzi schemes running on Ethereum. Based on these results, we propose to build a uniform platform to evaluate and monitor every created smart contract for early warning of scams.
The Metabolic Topography of Parkinsonism (1994)

We used [18F]fluorodeoxyglucose/positron emission tomography (18F-FDG/PET) and a statistical model of regional covariation to study brain topographic organization in parkinsonism. We studied 22 patients with Parkinson's disease (PD), 20 age-matched normal volunteers, and 10 age- and severity-matched patients with presumed striatonigral degeneration (SND). We used FDG/PET to calculate global, regional, and normalized metabolic rates for glucose (GMR, rCMRglc, rCMRglc/GMR). Metabolic parameters in the three groups were compared using an analysis of variance, with a correction for multiple comparisons, and discriminant analysis. The scaled subprofile model (SSM) was applied to the combined rCMRglc dataset to identify topographic covariance profiles that distinguish PD patients from SND patients and normals. GMR, rCMRglc, and rCMRglc/GMR were normal in PD; caudate and lentiform rCMRglc/GMR was reduced in the SND group (p < 0.01). SSM analysis of the combined group of patients and normals revealed a significant topographic profile characterized by increased metabolic activity in the lentiform nucleus and thalamus associated with decreased activity in the lateral frontal, paracentral, inferior parietal, and parietooccipital areas. Individual subject scores for this profile were significantly elevated in PD patients compared with normals and SND patients (p < 0.001) and discriminated the three groups. In the PD group, subject scores for this factor correlated with individual subject Hoehn and Yahr (H & Y) scores (p < 0.02), and with quantitative rigidity (p < 0.01) and bradykinesia (p < 0.03) ratings, but not with tremor ratings. SSM analysis of right-left metabolic asymmetries yielded a topographic contrast profile that accurately discriminated mildly affected PD patients (H & Y Stage I) from normals. Our findings demonstrate that abnormal topographic covariance profiles exist in parkinsonism. These profiles have potential clinical application as neuroimaging markers in parkinsonism.
Copper pillar bump technology progress overview (2011)

Fine-pitch copper pillar bump (CPB) for flip chip assembly is expanding in package applications for mobile electronic devices due to the need for smaller form factor, thin thickness, and the demand for better performance and lower power consumption. This paper updates the recent technology progress in fine-pitch copper pillar bump design, structure, fabrication, and applications in flip chip and wafer level package assemblies. With the advent of chip scale packages (CSP) and wafer level packages (WLP), the need for very fine-pitch micro bumps became apparent when Intel first started to use copper bumps in its microprocessor packages. There are many variants in IP-protected copper pillar bump designs, including IBM's MPS-C2 (metal post solder chip connection), Advanpack (APS), and Intel coated copper bumps. Wafer level processing steps to fabricate electrodeposited CPB are discussed here. Experimental studies to verify the advanced, fine-pitch CPB design using customer-designed daisy chain test vehicle wafers are presented. Lastly, some examples of applications of the current CPB technology for use in flip chip packages and 3D IC package integration using TSV (through silicon via) are described. Recent reliability findings on the fine-pitch CPB are also included.
Effect of Void Volume and Silver Loading on Strain Response of Electrical Resistance in Silver Flakes/Polyurethane Composite for Stretchable Conductors (2012)

Silver flakes/polyurethane composites have been reported as promising materials for stretchable conductors that can be easily fabricated by wet spinning or printing and yet have low electrical resistance. However, it is also important to control the effects of strain on electrical resistance: for example, stable electrical resistance over a range of strains would be useful for flexible displays or solar cells, whereas a positive or negative correlation between electrical resistance and strain would be useful for pressure or strain sensors. Here, we show that by controlling the void volume and silver loading, the electrical resistance is kept stable at a low value under deformation or is made to increase or decrease with strain. Cyclic tensile tests confirmed that these three behaviors were repeatable. This simple method for realizing three types of electrical behaviors could be very useful in stretchable electronics.
HIV broadly neutralizing antibodies and immunological tolerance (VAC7P.988) (2014)

Abstract It is generally thought that many human antibodies (Ab) that neutralize multiple clades of HIV-1 (bnAbs) are self-reactive. We previously identified kynureninase as the primary self-antigen recognized by 2F5, suggesting that generation of Ab to the 2F5 epitope of HIV-1 may be proscribed by immune tolerance, a notion supported by impaired B-cell development in mice expressing the 2F5 VH and VL regions. However, there is a lack of quantitative and systemic assessment of polyreactivity among HIV bnAbs. In addition, it is unknown whether other classes of HIV-1 bnAbs and non-neutralizing antibodies are also influenced by immunological tolerance. Here, we used protein microarrays to assess bnAb binding to over 9,600 human proteins and compared bnAb binding profiles to HIV non-neutralizing antibodies. We found that bnAbs as a class are more autoreactive and more polyreactive than non-neutralizers. Interestingly, 4 of 7 CD4bs bnAbs screened from 2 distinct lineages (VRC01, VRC02, CH103 and CH106) bind human protein ubiquitin ligase E3A (UBE3A). We confirmed this crossreactivity in ELISA, and demonstrated that UBE3A competitively inhibits gp120 binding to VRC01. Our results demonstrate that HIV-1 bnAbs are significantly more polyreactive and self-reactive than non-neutralizers, which may subject them to immunological tolerance control in vivo. Our identification of UBE3A as a self-antigen of bnAbs provides a mechanism for the rarity and delayed development of certain CD4bs bnAbs.
(13C)-Substituted erythronucleosides: synthesis and conformational analysis by proton and carbon-13 NMR spectroscopy (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXT(13C)-Substituted erythronucleosides: synthesis and conformational analysis by proton and carbon-13 NMR spectroscopyPaul C. Kline and Anthony S. SerianniCite this: J. Org. Chem. 1992, 57, 6, 1772–1777Publication Date (Print):March 1, 1992Publication History Published online1 May 2002Published inissue 1 March 1992https://pubs.acs.org/doi/10.1021/jo00032a032https://doi.org/10.1021/jo00032a032research-articleACS PublicationsRequest reuse permissionsArticle Views275Altmetric-Citations31LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Performance and environmental effects of forage production on sandy soils. V. Impact of grass understorey, slurry application and mineral N fertilizer on nitrate leaching under maize for silage (2006)

Abstract A field experiment with maize for silage was conducted to assess the effects of mineral nitrogen (N) fertilizer application rates (0, 50, 100, 150 kg ha −1 ), slurry application rates (0, 20, 40 m 3 ha −1 ) and the use of an understorey with perennial ryegrass on nitrate (NO 3 )‐leaching losses. Leachate was collected using ceramic suction cups. Soil mineral N (SMN) was determined to a depth of 90 cm at the end of the growing season. Higher levels of N supply with mineral fertilizer or slurry resulted in higher leaching losses. The grass understorey significantly reduced the losses. The amount of N lost to the groundwater was positively related to SMN at the end of the growing season, with leaching losses representing less than 0·45 of SMN on average. Leaching losses were positively related to the N surplus, which was calculated from the difference between N input (N from fertilizer, slurry and atmospheric deposition) and N output (N removed with maize herbage mass and bound in the understorey biomass in spring). In view of the large variation in weather conditions between the experimental years, it is suggested that for the sandy soils in this experiment N‐leaching losses under maize can be estimated satisfactorily from SMN and the calculated N surplus.
Guinea-pig kidney <i>β</i>-<i>N</i>-acetylgalactosaminyltransferase towards Tamm-Horsfall glycoprotein. Requirement of sialic acid in the acceptor for transferase activity (1983)

A beta-N-acetylgalactosaminyltransferase that preferentially transferred N-acetylgalactosamine to Sd(a-) Tamm-Horsfall glycoprotein was found in guinea-pig kidney microsomal preparations. This enzyme was kidney-specific and was able to transfer the sugar to other glycoproteins, such as fetuin and alpha 1-acidic glycoprotein. The presence of sialic acid in the acceptors was essential for the transferase activity when either glycoproteins or their Pronase glycopeptides were used as acceptors. Two glycopeptides (Tamm-Horsfall glycopeptides I and II) with a different carbohydrate composition were separated by DEAE-Sephacel chromatography from Pronase-digested Tamm-Horsfall glycoprotein. The amount of N-acetylgalactosamine transferred to glycopeptides by the enzyme correlated with their degree of sialylation. Enzymic digestion of N-[14C]acetylgalactosamine-labelled Tamm-Horsfall glycopeptide II showed that the transferred sugar was susceptible to beta-N-hexosaminidase. The amount of sugar cleaved by beta-hexosaminidase was strongly increased when the labelled Tamm-Horsfall glycopeptide II was pretreated with mild acid hydrolysis, a procedure that removed the sialic acid residues. Alkaline borohydride treatment of the labelled Tamm-Horsfall glycopeptide II did not release radioactivity, thus indicating that enzymic glycosylation took place at the N-asparagine-linked oligosaccharide units of Tamm-Horsfall glycoprotein.
WIF-B cells: an in vitro model for studies of hepatocyte polarity. (1993)

We have evaluated the utility of the hepatoma-derived hybrid cell line, WIF-B, for in vitro studies of polarized hepatocyte functions. The majority (> 70%) of cells in confluent culture formed closed spaces with adjacent cells. These bile canalicular-like spaces (BC) accumulated fluorescein, a property of bile canaliculi in vivo. By indirect immunofluorescence, six plasma membrane (PM) proteins showed polarized distributions similar to rat hepatocytes in situ. Four apical PM proteins were concentrated in the BC membrane of WIF-B cells. Microtubules radiated from the BC (apical) membrane, and actin and foci of gamma-tubulin were concentrated in this region. The tight junction-associated protein ZO-1 was present in belts marking the boundary between apical and basolateral PM domains. We explored the functional properties of this boundary in living cells using fluorescent membrane lipid analogs and soluble tracers. When cells were incubated at 4 degrees C with a fluorescent analog of sphingomyelin, only the basolateral PM was labeled. In contrast, when both PM domains were labeled by de novo synthesis of fluorescent sphingomyelin from ceramide, fluorescent lipid could only be removed from the basolateral domain. These data demonstrate the presence of a barrier to the lateral diffusion of lipids between the PM domains. However, small soluble FITC-dextrans (4,400 mol wt) were able to diffuse into BC, while larger FITC-dextrans were restricted to various degrees depending on their size and incubation temperature. At 4 degrees C, the surface labeling reagent sNHS-LC-biotin (557 mol wt) had access to the entire PM, but streptavidin (60,000 mol wt), which binds to biotinylated molecules, was restricted to only the basolateral domain. Such differential accessibility of well-characterized probes can be used to mark each membrane domain separately. These results show that WIF-B cells are a suitable model to study membrane trafficking and targeting in hepatocytes in vitro.
The endocannabinoid system: a general view and latest additions (2004)

After the discovery, in the early 1990s, of specific G-protein-coupled receptors for marijuana's psychoactive principle Delta(9)-tetrahydrocannabinol, the cannabinoid receptors, and of their endogenous agonists, the endocannabinoids, a decade of investigations has greatly enlarged our understanding of this altogether new signalling system. Yet, while the finding of the endocannabinoids resulted in a new effort to reveal the mechanisms regulating their levels in the brain and peripheral organs under physiological and pathological conditions, more endogenous substances with a similar action, and more molecular targets for the previously discovered endogenous ligands, anandamide and 2-arachidonoylglycerol, or for some of their metabolites, were being proposed. As the scenario becomes subsequently more complicated, and the experimental tasks to be accomplished correspondingly more numerous, we briefly review in this article the latest 'additions' to the endocannabinoid system together with earlier breakthroughs that have contributed to our present knowledge of the biochemistry and pharmacology of the endocannabinoids.
Semiparametric Models for Cumulative Incidence Functions (2004)

In analyses of time-to-failure data with competing risks, cumulative incidence functions may be used to estimate the time-dependent cumulative probability of failure due to specific causes. These functions are commonly estimated using nonparametric methods, but in cases where events due to the cause of primary interest are infrequent relative to other modes of failure, nonparametric methods may result in rather imprecise estimates for the corresponding subdistribution. In such cases, it may be possible to model the cause-specific hazard of primary interest parametrically, while accounting for the other modes of failure using nonparametric estimators. The cumulative incidence estimators so obtained are simple to compute and are considerably more efficient than the usual nonparametric estimator, particularly with regard to interpolation of cumulative incidence at early or intermediate time points within the range of data used to fit the function. More surprisingly, they are often nearly as efficient as fully parametric estimators. We illustrate the utility of this approach in the analysis of patients treated for early stage breast cancer.
How Electrolytes Influence Battery Safety (2012)

Electrolytes have been shown to be a major source of poor safety response of Li ion cells. The physical hazards associated with conventional LiPF6 and carbonate-based electrolytes are well documented and include high volume gas decomposition products at elevated temperature, large combustion enthalpy and flammability of solvent vapor. However, the physical and health hazards of the decompositions products can be often overlooked. Electrolyte additives proposed to reduce gas generation and mitigate flammability have not gained much traction, in general, because of the trade-off in cell performance. To maintain cell performance, quantities of additives are generally introduced in fractions <10% which significantly decreases the efficacy of the additive. New additives need to be developed in order to strike the balance between improving abuse tolerance and maintaining performance.
在生活与幻想间游走专访插画家、概念设计师“天涯寻欢”李涛 (2011)

被古龙小说《多情剑客无情剑》中优雅强悍的“小李飞刀”深深吸引的李涛，随性地把自己的笔名叫做“李寻欢”，现在这个在国际上屡获殊荣，享有盛誉的优秀CG艺术家，又把自己富有戏剧性的人生快车开上了“北京”这条高速路，继续前行。