gebhart/patrepeval huggingface dataset:
Cloning, sequencing, and expression of the N-acyl-D-mannosamine dehydrogenase gene from Flavobacterium sp. strain 141-8 in Escherichia coli (1991)

The gene coding for N-acyl-D-mannosamine dehydrogenase (NAM-DH) from Flavobacterium sp. strain 141-8 was cloned and expressed under the control of a lac promoter in Escherichia coli JM109. The DNA sequence of the gene was determined, and an open reading frame encoding a polypeptide composed of 272 amino acid residues (Mr, 27,473) was identified. The E. coli transformants which showed over 200-fold higher NAM-DH activity than did the Flavobacterium strain produced the enzyme as a protein fused with beta-galactosidase. Despite being a fusion, NAM-DH produced by E. coli transformants appeared unchanged in pH optimum, Km, and substrate specificity from Flavobacterium sp. strain 141-8. This newly recombinant enzyme may be applicable to the quantitative determination of sialic acid in serum.
Development and evaluation of a navigation grade resonator fiber optic gyroscope (2015)

This paper will discuss the development and evaluation of a resonator fiber optic gyroscope (RFOG) for precision navigation applications. This RFOG is based on an optical resonator comprised of hollow core optical fiber, a novel multi-frequency laser source and highly integrated free space and guided wave optical subsystems. Performance evaluation was completed and the results are presented.
Coating with a Modular Bone Morphogenetic Peptide Promotes Healing of a Bone-Implant Gap in an Ovine Model (2012)

Despite the potential for growth factor delivery strategies to promote orthopedic implant healing, there is a need for growth factor delivery methods that are controllable and amenable to clinical translation. We have developed a modular bone growth factor, herein termed "modular bone morphogenetic peptide (mBMP)", which was designed to efficiently bind to the surface of orthopedic implants and also stimulate new bone formation. The purpose of this study was to coat a hydroxyapatite-titanium implant with mBMP and evaluate bone healing across a bone-implant gap in the sheep femoral condyle. The mBMP molecules efficiently bound to a hydroxyapatite-titanium implant and 64% of the initially bound mBMP molecules were released in a sustained manner over 28 days. The results demonstrated that the mBMP-coated implant group had significantly more mineralized bone filling in the implant-bone gap than the control group in C-arm computed tomography (DynaCT) scanning (25% more), histological (35% more) and microradiographic images (50% more). Push-out stiffness of the mBMP group was nearly 40% greater than that of control group whereas peak force did not show a significant difference. The results of this study demonstrated that mBMP coated on a hydroxyapatite-titanium implant stimulates new bone formation and may be useful to improve implant fixation in total joint arthroplasty applications.
Generation of hexagonal close‐packed ring‐shaped structures using an optical vortex (2021)

An optical vortex possesses a ring-shaped spatial profile and orbital angular momentum (OAM) owing to its helical wavefront. This form of structured light has garnered significant attention in recent years, and it has enabled new investigations in fundamental physics and applications. One such exciting application is laser-based material transfer for nano-/micro-fabrication. In this work, we demonstrate the application of a single-pulse optical vortex laser beam for direct printing of ring-shaped structures composed of hexagonal close-packed, mono-/multi-layered nanoparticles which exhibit 'structural color'. We compare and contrast the interaction of the vortex beam with both dielectric and metallic nanoparticles and offer physical insight into how the OAM of vortex beams interacts with matter. The demonstrated technique holds promise for not only photonic-based nano-/micro-fabrication, but also as a means of sorting particles on the nanoscale, a technology which we term 'optical vortex nanoparticle sorting'.
Interferon Induction and Macrophage Activation by a Mycovirus Double-Stranded RNA/ Tobramycin Complex Following Treatment with Human Serum (1982)

Double-stranded RNA (dsRNA) molecules, generally effective inducers of interferon (IFN), have a weak potency in man apparently because of the presence of a serum RNAase which quickly inactivates extracellular dsRNA. We have discovered that tobramycin, an aminoglycoside, protects Penicillium chrysogenum mycovirus dsRNA (PCMdsRNA) from the degradative action of the nuclease. Exposure of the dsRNA/tobramycin complex to human serum results in some degradation, but still permits the production of significantly high titers of IFN upon injection into mice. Intraperitoneal (i.p.) treatment of CFI mice with both dsRNA and dsRNA/tobramycin complex activated macrophages to inhibit the growth of P815 mastocytoma target cells. Following in vitro treatment with human serum, free dsRNA failed to activate peritoneal macrophages in vivo under conditions where this activity of dsRNA/tobramycin complex was retained. By varying the ratio of moles of tobramycin (as free base) to the moles of RNA phosphorous, toxicity can be minimized, while retaining the biologic activities of dsRNA.
Adding shadows to a texture-based volume renderer (1998)

Texture-breed volume rendering is a technique to efficiently vistiti volumetric data using texture mapping hardware. k this paper we present nn rdgorithm, that extends this approach to render shadows for the voltuna The rdgonthrn takes advantage of&t framebuffer opemdons modem graphics hardware offers, but does not depend on zIy spdd purpose hardware.
Property Evaluation and Damage Evolution of Environmental Barrier Coatings and Environmental Barrier Coated SiC/SiC Ceramic Matrix Composite Sub-Elements (2014)

This paper describes recent development of environmental barrier coatings on SiC/SiC ceramic matrix composites. The creep and fatigue behavior at aggressive long-term high temperature conditions have been evaluated and highlighted. Thermal conductivity and high thermal gradient cyclic durability of environmental barrier coatings have been evaluated. The damage accumulation and complex stress-strain behavior environmental barrier coatings on SiCSiC ceramic matrix composite turbine airfoil subelements during the thermal cyclic and fatigue testing of have been also reported.
Developments of a Sewage Treatment Systems Using Superconducting Magnetic Separation (2005)

We have developed a sewage treatment system using a superconducting magnetic separation that combines high gradient magnetic separation using a continuously rotating magnetic filter with a superconducting magnet. We tested the magnetic separator to remove ferromagnetic floc, when ferromagnetic powder and coagulant are added to the influent water. The experiment showed that the magnetic filter can be practically used in this treatment system and that when the separator operates at a high speed (i.e. about 50 times faster than normal speed of conventional technology), its performance improved 90%.
Synthesis and biological evaluation of antagonists of growth hormone-releasing hormone with high and protracted <i>in vivo</i> activities (1999)

Some antagonists of human growth hormone-releasing hormone (hGH-RH) synthesized previously were shown to inhibit in vivo proliferation of various human cancers in nude mice. However, the activity of these analogs requires an increase to assure clinical efficacy. In an attempt to prepare hGH-RH antagonists with a high and protracted activity, we synthesized and biologically tested 22 antagonistic analogs of hGH-RH(1-29)NH2. The ability of the antagonists to inhibit hGH-RH-induced GH release was evaluated in vitro in a superfused rat pituitary system, as well as in vivo after i.v. injection into rats. The binding affinity of the peptides to GH-RH receptors also was determined. All antagonistic analogs had the common core sequence [PhAc-Tyr1,D-Arg2, Phe(4-Cl)6 (para-chlorophenylalanine), Abu15 (alpha-aminobutyric acid), Nle27]hGH-RH(1-29)NH2 and contained Arg, D-Arg, homoarginine (Har), norleucine (Nle), and other substitutions. The following analogs were determined to have a high and/or protracted antagonistic activity: [PhAc-Tyr1,D-Arg2,Phe(4-Cl)6,Arg9,Abu15,Nle27, D-Arg29]hGH-RH(1-29)NH2 (JV-1-10), [PhAc-Tyr1,D-Arg2,Phe(4-Cl)6, Abu15,Nle27,D-Arg28,Har29]hGH-RH(1-29)NH2 (MZ-6-55), [PhAc-Tyr1, D-Arg2,Phe(4-Cl)6,Arg9,Abu15,Nle27,D-Arg28,Har29 ]hGH-RH(1-29)NH2 (JV-1-36), and [PhAc-Tyr1,D-Arg2,Phe(4-Cl)6,Har9,Tyr(Me)10,Abu15, Nle27,D-Arg28,Har29]hGH-RH(1-29)NH2 (JV-1-38). Among the peptides tested, analog JV-1-36 showed the highest GH-RH antagonistic activity in vitro and also induced a strong and prolonged inhibition of GH release in vivo for at least 30 min. The antagonist JV-1-38 was slightly less potent than JV-1-36 both in vitro and in vivo but proved to be very long-acting in vivo, suppressing the GH-RH-induced GH release even after 60 min. High and protracted in vivo activities of these antagonists indicate an improvement over earlier GH-RH analogs. Some of these hGH-RH antagonists could find clinical applications in the treatment of cancers dependent on insulin-like growth factors I and II.
Ultrahigh-resolution multicolor colocalization of single fluorescent nanocrystals. (2001)

A new method for <i>in vitro</i> and possibly <i>in vivo</i> ultrahigh-resolution colocalization and distance measurement between biomolecules is described, based on semiconductor nanocrystal probes. This ruler bridges the gap between FRET and far-field (or near-field scanning optical microscope) imaging and has a dynamic range from few nanometers to tens of micrometers. The ruler is based on a stage-scanning confocal microscope that allows the simultaneous excitation and localization of the excitation point-spread-function (PSF) of various colors nanocrystals while maintaining perfect registry between the channels. Fit of the observed diffraction and photophysics-limited images of the PSFs with a two-dimensional Gaussian allows one to determine their position with nanometer accuracy. This new high-resolution tool opens new windows in various molecular, cell biology and biotechnology applications.
Strategies for the Synthesis of N‐(Azacycloalkyl)bisindolylmaleimides: Selective Inhibitors of PKCβ. (2003)

Abstract For Abstract see ChemInform Abstract in Full Text.
Cardiovascular autonomic nervous system dysfunction of chagasic etiology: Proposal for a new approach to treatment (1994)

The authors review the evidence supporting the therapeutic use of gangliosides in autonomic neuropathies, with particular reference to those occurring in Chagas' disease.
Generation of word graphs in statistical machine translation (2002)

Statistical machine translation systems usually compute the single sentence that has the highest probability according to the models that are trained on data. We describe a method for constructing a word graph to represent alternative hypotheses in an efficient way. The advantage is that these hypotheses can be rescored using a refined language or translation model. Results are presented on the German-English Verbmobil corpus.
Hyperplane Culling for Stochastic Rasterization (2012)

We present two novel culling tests for rasterization of simultaneous depth of field and motion blur. These tests efficiently reduce the set of xyuvt samples that need to be coverage tested within a screen space tile. The first test finds linear bounds in ut- and vt-space using a separating line algorithm. We also derive a hyperplane in xyuvtspace for each triangle edge, and all samples outside of these planes are culled in our second test. Based on these tests, we present an efficient stochastic rasterizer, which has substantially higher sample test efficiency and lower arithmetic cost than previous tile-based stochastic rasterizers.
The Mycoplasma arthritidis superantigen MAM: purification and identification of an active peptide (1994)

The prototypical superantigen MAM is an extracellular T-cell mitogen produced by Mycoplasma arthritidis, an organism which causes chronic proliferative arthritis of rodents. We here describe purification of MAM to homogeneity. Pure MAM exhibits all of the major properties previously described for partially purified MAM, including preference for H-2E molecules in presention to T cells, V beta T-cell receptor specificity for T-cell activation, and in vivo inhibition of T-cell functions but enhancement of B-cell activity as mediated by the superantigen bridge. Edman degradation of pure MAM gave a 54-residue partial amino-terminal sequence. The oligopeptide MAM15-31-C, synthesized according to the Edman sequence, blocked mitogenicity of MAM and supported assignment of the amino acid sequence.
Mechanism of Resist Pattern Collapse (1993)

In this paper, the mechanism of resist pattern collapse during the resist development process is investigated by the use of atomic force microscope measurement of a pattern in the rinse liquid. The resist pattern collapse occurs during the rinse liquid is dried off, and the cause of collapse is the capillary force of the rinse liquid.
Studies on Disease-Modifying Antirheumatic Drugs. III. Bone Resorption Inhibitory Effects of Ethyl 4-(3,4-Dimethoxyphenyl)-6,7-dimethoxy-2-(1,2,4-triazol-1-ylmethyl)quinoline-3-carboxylate (TAK-603) and Related Compounds. (1999)

In the course of our studies aimed at obtaining new drugs for treatment of bone and joint diseases, chemical modification of the potent bone resorption inhibitors justicidins, was performed and various naphthalene lactones, quinoline lactones and quinoline derivatives bearing an azole moiety at the side chain were prepared. Their inhibitory effects on bone resorption were evaluated by Raisz's method, and several compounds, including ethyl 4-(3,4-dimethoxyphenyl)-6,7-dimethoxy-2-(1,2,4-triazol-1-ylmeth yl)quinoline -3-carboxylate (6c, TAK-603), were found to have activities comparable with or superior to the justicidins. The 4-(3-isopropoxy-4-methoxy)-phenyl derivative (6d), in particular, displayed a marked increase in potency. TAK-603 and compound 6d were very effective in preventing osteoclast formation and bone resorption by mature osteoclasts. Further, TAK-603 was shown to be effective in preventing bone loss in ovariectomized mice.
Data from Circulating miRNAs as Surrogate Markers for Circulating Tumor Cells and Prognostic Markers in Metastatic Breast Cancer (2023)

<div>Abstract<p><b>Purpose:</b> The use of circulating tumor cells (CTC) as a prognostic marker in metastatic breast cancer (MBC) has been well established. However, their efficacy and accuracy are still under scrutiny mainly because of methods of their enrichment and identification. We hypothesized that circulating miRNAs can predict the CTC status of patients with MBC, and tested for the same. Furthermore, we aimed at establishing a panel of circulating miRNAs capable of differentiating MBC cases from healthy controls.</p><p><b>Experimental Design:</b> Circulating miRNAs from plasma of CTC-positive and CTC-negative patients with MBC, and healthy controls, were profiled by TaqMan Human MicroRNA arrays. Candidates from the initial screen were validated in an extended cohort of 269 individuals (61 CTC-positive, 72 CTC-negative, 60 CTC-low MBC cases, and 76 controls).</p><p><b>Results:</b> CTC-positive had significantly higher levels of miR-141, miR-200a, miR-200b, miR-200c, miR-203, miR-210, miR-375, and miR-801 than CTC-negative MBC and controls (<i>P</i> < 0.00001), whereas miR-768-3p was present in lower amounts in MBC cases (<i>P</i> < 0.05). miR-200b was singled out as the best marker for distinguishing CTC-positive from CTC-negative patients (AUC 0.88). We identified combinations of miRNAs for differentiating MBC cases from controls (AUC 0.95 for CTC-positive; AUC 0.78 for CTC-negative). Combinations of miRNAs and miR-200b alone were found to be promising prognostic marker for progression-free and overall survival.</p><p><b>Conclusion:</b> This is the first study to document the capacity of circulating miRNAs to indicate CTC status and their potential as prognostic markers in patients with MBC. <i>Clin Cancer Res; 18(21); 5972–82. ©2012 AACR</i>.</p></div>
Identification of new and common mutations in the <i>EPM2A gene in Lafora disease</i> (2000)

Lafora disease is a teenage onset progressive myoclonus epilepsy caused by mutations in the EPM2A gene. In this report, we describe new mutations within EPM2A, review the known mutations to date to identify the most common, and describe three simple tests for prenatal and carrier screening.
A novel switched interferometric quantum key distribution system (2007)

A novel system for ultra-long-distance quantum key distribution in optical fiber, incorporating ultra-low-noise transition-edge sensor (TES) photodetectors, is described. Integration of the TES detectors into the system was facilitated with a unique optically switched interferometer design. The performance of the system over 101 km of dark, single-mode fiber at 1550 nm and a clock rate of 1 MHz is described. Secret-key bits were produced after error correction and privacy amplification when using mean photon numbers of 0.01, 0.0148, 0.02, 0.0304, and 0.2 photons/pulse at the output of the transmitter. At a mean photon number of 0.1 photons per pulse at the transmitter, a transmission line loss of 29.92 dB, roughly equivalent to 150 km of optical fiber, could be tolerated and secret bits extracted from the transmitted key.
Amino acids and peptides. Part 19. Synthesis of β-1- and β-2-adamantyl aspartates and their evaluation for peptide synthesis (1988)

β-1- and β-2-Adamantyl aspartates [H-Asp(O-1-Ada)-OH and H-Asp(O-2-Ada)-OH] have been synthesized and their properties examined. Although the 1-Ada group is labile to TFA, the 2-Ada group is unaffected during TFA treatment, but easily removable by methanesulphonic acid (MSA) at room temperature within 5 min. Both groups are unaffected by treatment with 55% piperidine under conditions which easily cleave the fluoren-9-ylmethoxycarbonyl (Fmoc) group from an α-amino group. Both groups can suppress aspartimide formation as a side reaction under acidic and basic conditions during the synthesis of aspartyl peptides. β-1-or β-2-Adamantyl aspartates may be applicable to solidphase peptide synthesis in combination with Fmoc or Boc as an Nα-protecting group, respectively. Some properties of the aspartimide moiety are described.
Radon and Progeny Detection Using Tensioned Metastable Fluid Detectors (2019)

Radon and other alpha-emitting nuclides in air can be a significant risk for health hazards in the workplace and in dwellings. Radon gas in US homes causes over 21,000 deaths from lung cancer annually, according to the US Environmental Protection Agency (US EPA). Alpha-emitting radionuclides from airborne contamination in the nuclear industry also constitute a known safety and security-safeguards related issue. Purdue University, along with Sagamore Adams Laboratories LLC, have developed the tensioned metastable fluid detector (TMFD) sensor technology for general-purpose alpha-neutron-fission. For radon detection, radon gas in air is sparged through the TMFD detection fluid, after which this radon-bearing fluid is placed into a tensioned metastable state. In this metastable state, an audible-visible cavitation (alpha radiation) detection event is created and electronically recorded. Tailoring the level of tension enables spectroscopic information through energy discrimination of radon and its progeny; This is also applicable to other alpha emitting isotopes (Pu, Cm, U…). TMFDs boast high intrinsic detection efficiencies (>95%) for alpha spectroscopy while remaining blind to background beta and gamma radiation even in ~10 R h fields. The immunity to beta and gammas allows for reusability of the detection fluid through immunity to beta buildup from thoron and radon progeny. TMFD technology was used to measure the concentration of radon and radon progeny in air for concentrations from 74 Bq m to 740 Bq m. The system measures radon concentration between these levels within 24 h with an intrinsic relative error (IRE) of ± 15% according to the standards set forth by the American Association of Radon Scientists and Technicians-National Radon Proficiency Program (AARST-NRPP) Device Evaluation Program (DEP). Precision evaluation defined using the relative standard deviation set by the AARST-NRPP measured a deviation of <5%, which exceeds the requirement of <25%. Temperature effects from 10°C and 27°C were assessed and corrected for dynamically. A radon decay correction method was introduced to correct for the radon activity decrease in the detection fluid over the counting period due to the ~3.8 d half-life. Blind testing was also successfully performed* with standards from the ; within 6 h, the TMFD-based technology accurately measured the concentration of radon to within 20% of the reference standards. Non-condensing relative humidity levels up to 95% per AARST-NRPP were assessed for and were found to have no impact on radon detection. This work improves upon the previous work by splitting the calibration curve into two distinct regions and adds additional information pertaining to the effects of relative humidity on the system.
The Differential Diagnosis of Hairy Cell Leukemia with a Panel of Monoclonal Antibodies (1985)

A panel of monoclonal antibodies reactive with normal human lymphoid cells and with hairy cells has been applied to the immunocytochemical analysis of hairy cell leukemia. Staining was performed by immunoenzymatic methods on frozen sections of bone marrow trephines and extramedullary tissues and on cell smears. Hairy cells reacted with antibodies against HLA-DR, leukocyte common antigen, B-cell antigens (antibodies To15 and B1) and with three anti-hairy cell monoclonal antibodies (S-HCL3, HC1, and HC2). Neoplastic cells in other B-cell lymphoproliferative disorders also expressed HLA-DR, leukocyte common, and B-cell antigens but were consistently negative for the antigen detected by monoclonal antibody S-HCL3. Furthermore, hairy cells differed from other neoplastic B-cells in that they were unreactive with monoclonal antibodies against C3b receptors, anti-Leu-1, Tü1, Tü33, and lacked a meshwork of dendritic reticulum cells. These findings establish a distinctive antigenic phenotype for hairy cell leukemia and indicate that it may be diagnosed reliably by immunoenzymatic labeling of tissue sections or cell smears.
Superplasticity of strip cast aluminum alloys. (1985)

Eutectic Al-12%Si, Al-13%Mg2Si and Al-6%Ni alloys, Al-Mg-Mn-Cr, Al-Mn and Al-Mg-Si-Mn-Cr alloys were directly rolled from the liquid state between rotating twin chill rolls into plates 6mm in thickness and were cold rolled into sheets about 1mm in thickness. Bulge forming superplasticity tests were carried out. Eutectic particles are uniformly refined and transition elements effective to obstruct recrystallization are supersaturated to a greater degree in the sheets solidified at high cooling rates 300 to 600K/s. Such sheets are preferable in superplasticity to the usual sheets. Refined eutectic particles in the alloys having low volume fraction of eutectic particles play an important role in obstructing coarsening of recrystallizaed grains similarly as precipitates. The sheets containing such particles stable at elevated temperatures as Mg2Si have excellent superplasticity even at higher temperatures. Delayed transformation of the particles at elevated temperatures is effective to keep the superplastic condition.