gebhart/patrepeval huggingface dataset:
Adenoviral Gene Transfer of Stromal Cell–Derived Factor-1 to Murine Tumors Induces the Accumulation of Dendritic Cells and Suppresses Tumor Growth (2006)

The human CXC chemokine, stromal cell-derived factor 1 (SDF-1alpha), is known to function in vitro as a chemotactic factor for lymphocytes, monocytes, and dendritic cells. In the context that dendritic cells are powerful antigen-presenting cells, we hypothesized that adenoviral gene transfer of SDF-1alpha to tumors might inhibit growth of preexisting tumors through attracting dendritic cells to the tumor. AdSDF-1alpha mediated the expression of SDF-1alpha mRNA and protein in A549 cells in vitro, and the supernatant of the AdSDF-1alpha-infected A549 cells showed chemotactic activity for dendritic cells. When syngeneic murine CT26 colon carcinoma tumors (BALB/c) and B16 melanoma and Lewis lung cell carcinoma (C57Bl/6) were injected with AdSDF-1alpha (5 x 10(8) plaque-forming units), there was an accumulation of dendritic cells and CD8(+) cells within the tumor and significant inhibition of tumor growth compared with tumors injected with PBS or AdNull (control vector). The injection of AdSDF-1alpha into tumors induced the inflammatory enlargement and the accumulation of dendritic cells in the draining lymph node. Intratumoral AdSDF-1alpha administration elicited tumor-specific CTLs and adoptive transfer of splenocytes from AdSDF-1alpha-treated mice resulted in the elongation of survival after tumor challenge. Interestingly, in wild-type and CD4(-/-) mice but not in CD8(-/-) mice, AdSDF-1alpha inhibited the growth of the tumor. These observations suggest that adenoviral gene transfer of SDF-1alpha may be a useful strategy to accumulate dendritic cells in tumors and evoke antitumor immune responses to inhibit tumor growth.
The N-Terminal Extracellular Domain 23−60 of the Calcitonin Receptor-Like Receptor in Chimeras with the Parathyroid Hormone Receptor Mediates Association with Receptor Activity-Modifying Protein 1 (2005)

The calcitonin receptor-like receptor (CLR) requires the associated receptor activity-modifying protein (RAMP)1 to reveal a calcitonin gene-related peptide (CGRP) receptor. Here, the subdomain of the CLR that associates with RAMP1 has been identified in chimeras between the CLR and the parathyroid hormone (PTH) receptor 1 (PTHR). The PTHR alone does not interact with RAMP1. RAMP1 requires the CLR for its transport to the cell surface. Thus, receptor-dependent RAMP1 delivery to the plasma membrane and coimmunoprecipitation from the cell surface were used as measures for receptor/RAMP1 interaction. Several chimeric CLR-PTHR included the N-terminal amino acids 23-60 of the CLR transported RAMP1 to the surface of COS-7 cells much like the intact CLR. Moreover, RAMP1 coimmunoprecipitated with these receptors from the cell surface. A CLR deletion mutant, consisting of the N-terminal extracellular domain, the first transmembrane domain, and the C-terminal intracellular region, revealed the same results. Cyclic AMP was stimulated by CGRP in CLR/RAMP1 expressing cells (58 +/- 19-fold, EC(50) = 0.12 +/- 0.03 nM) and by PTH-related protein in cells expressing the PTHR (50 +/- 10-fold, EC(50) = 0.25 +/- 0.03 nM) or a PTHR with the N-terminal amino acids 23-60 of the CLR (23 +/- 5-fold, EC(50) > 1000 nM). Other chimeric CLR-PTHR were inactive. In conclusion, structural elements in the extreme N-terminus of the CLR between amino acids 23-60 are required and sufficient for CLR/RAMP1 cotransport to the plasma membrane and heterodimerization.
The RNA-Binding Protein Rrm4 is Essential for Efficient Secretion of Endochitinase Cts1 (2011)

Long-distance transport of mRNAs is crucial in determining spatio-temporal gene expression in eukaryotes. The RNA-binding protein Rrm4 constitutes a key component of microtubule-dependent mRNA transport in filaments of Ustilago maydis. Although a number of potential target mRNAs could be identified, cellular processes that depend on Rrm4-mediated transport remain largely unknown. Here, we used differential proteomics to show that ribosomal, mitochondrial, and cell wall-remodeling proteins, including the bacterial-type endochitinase Cts1, are differentially regulated in rrm4Δ filaments. In vivo UV crosslinking and immunoprecipitation and fluorescence in situ hybridization revealed that cts1 mRNA represents a direct target of Rrm4. Filaments of cts1Δ mutants aggregate in liquid culture suggesting an altered cell surface. In wild type cells Cts1 localizes predominantly at the growth cone, whereas it accumulates at both poles in rrm4Δ filaments. The endochitinase is secreted and associates most likely with the cell wall of filaments. Secretion is drastically impaired in filaments lacking Rrm4 or conventional kinesin Kin1 as well as in filaments with disrupted microtubules. Thus, Rrm4-mediated mRNA transport appears to be essential for efficient export of active Cts1, uncovering a novel molecular link between mRNA transport and the mechanism of secretion.
PERF join (1995)

Article Free Access Share on PERF join: an alternative to two-way semijoin and bloomjoin Authors: Zhe Li Department of Computer Science, Columbia University, New York, NY Department of Computer Science, Columbia University, New York, NYView Profile , Kenneth A. Ross Department of Computer Science, Columbia University, New York, NY Department of Computer Science, Columbia University, New York, NYView Profile Authors Info & Claims CIKM '95: Proceedings of the fourth international conference on Information and knowledge managementDecember 1995 Pages 137–144https://doi.org/10.1145/221270.221360Published:02 December 1995Publication History 26citation554DownloadsMetricsTotal Citations26Total Downloads554Last 12 Months45Last 6 weeks5 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
Hostages to fortune (2012)

Drawing upon a growing corpus of knowledge, this article examines the extent and impact of violence on health and social care staff. Whilst individual and collective strategies for preventing and avoiding violence are explored, the article also examines themes which emerge from the research literature and considers some of the complexities surrounding this area. Current policies with respect to violence against health and social care staff are the subject of reflection as are future possibilities for research.
Architectural Adaptation as Praxis (2022)

Since industrialization, modern architecture has appropriated the notion of adaptation. Defined as the adjustment of a building to the environment and its users, architectural adaptation has been mainly carried out via a narrow technological approach. Thus, digitalization has emerged as the latest ‘smart’ update. The limits of technological adaptation become especially evident with architecture in aiming to solve an ecological and social crisis on both a global and local level. In this paper, we argue for reconceptualizing adaptivity in architecture to (re)integrate processual, social, and aesthetic dimensions. We propose a new architectural understanding of adaptivity that includes currently excluded agents and involves them in communication and adaptation processes. As we focus on the intertwining of technical developments and cultural practices, that is, the interactions of human and non-human agents in architecture, we seek to describe architectural adaptation as an inclusive spatial praxis. This may aid in inventing new ways of life built upon sustainable nature-culture-technology relationships within society.
Hepatocyte Nuclear Factor-4α P2 Promoter Haplotypes Are Associated With Type 2 Diabetes in the Japanese Population (2006)

Hepatocyte nuclear factor (HNF)-4alpha is a transcription factor known as a key molecule in the development and functions of the beta-cells. In a previously performed genome-wide scan of Japanese type 2 diabetic sibpairs, we observed linkage of type 2 diabetes to chromosome 20q12-q13, a region in which the HNF4A gene is located. Recent studies have reported associations between type 2 diabetes and polymorphisms in the P2 promoter region specific to beta-cells. In this study, we attempted to assess whether the HNF4A gene plays a role in the genetic susceptibility to type 2 diabetes in the Japanese population by analyzing polymorphisms and haplotypes of the HNF4A gene. Linkage disequilibrium across the P2 promoter region was preserved in the Japanese population, consistent with previous reports. Although none of the individual polymorphisms examined showed any significant association with type 2 diabetes, we found very strong evidence of the association between type 2 diabetes and the haplotype consisting of two polymorphisms in the P2 promoter region of the HNF4A gene (P = 3.82 x 10(-4)). In contrast, there was no association between type 2 diabetes and haplotypes consisting of polymorphisms not located in the P2 promoter region, suggesting that the type 2 diabetes susceptibility loci are localized in the P2 promoter region of the HNF4A gene. The association was replicated using two additional cohorts (P = 1.51 x 10(-4) and 0.019, respectively). The results of the present analysis revealed that the HNF4A gene might be a type 2 diabetes susceptibility gene common to different ethnic groups. The study also suggested the possible existence of an as-yet-unidentified but functional polymorphism in the P2 promoter region of the HNF4A gene that directly influences susceptibility to type 2 diabetes.
Area Selective Deposition of Metals from the Electrical Resistivity of the Substrate (2021)

Area selective deposition (ASD) of films only on desired areas of the substrate opens for less complex fabrication of nanoscaled electronics. We show that a newly developed CVD method, where plasma electrons are used as the reducing agent in deposition of metallic thin films, is inherently area selective from the electrical resistivity of the substrate surface. When depositing iron with the new CVD method, no film is deposited on high-resistivity SiO<sub>2</sub> surfaces whereas several hundred nanometers thick iron films are deposited on areas with low resistivity, obtained by adding a thin layer of silver on the SiO<sub>2</sub> surface. On the basis of such a scheme, we show how to use the electric resistivity of the substrate surface as an extension of the ASD toolbox for metal-on-metal deposition.
A Simple Mechanistic Way to Increase the Survival of Mammalian Cells During Processing for Dry Storage (2010)

Recently, there has been considerable interest in developing processing methods that enable storage of cells in a dry state. Most of these studies describe cell viability following processing as a function of the final moisture content reached or the duration of drying. Recently, a cumulative osmotic stress model has been proposed, which takes both final moisture content and duration of drying into consideration in an effort to account for the effects of cumulative processing stresses. The present study demonstrates the applicability of this approach and elucidates a simple mechanistic technique to reduce cumulative osmotic stress during processing. Mouse macrophage cells (J774) were exposed to increasing concentrations of trehalose-containing 0.33× phosphate-buffered saline (PBS) by step-changing the extracellular solution in 2 increments of 0.7 Osm, using only trehalose as the additive solute. Three minutes was provided for equilibration prior to drying in a traditional low-humidity chamber. The data were compared with that of cells dried directly in isotonic 0.2 M trehalose in 0.33× PBS. Following dehydration, cells were rehydrated and viability was assessed 45 min postrehydration using a combination of trypan blue staining for membrane integrity of detached cells and calcein AM-propidium iodide fluorescence assay for live-dead staining of the attached cells. Cells that were preprocessed to higher trehalose concentrations in step changes prior to drying had higher viability scores at comparable final moisture levels when compared with cells dried in iso-osmotic solution, up to a limit of ∼8 Osm, at which point cells processed by both methods approached zero viability.
Pose estimation using both points and lines for geo-localization (2011)

This paper identifies and fills the probably last two missing items in minimal pose estimation algorithms using points and lines. Pose estimation refers to the problem of recovering the pose of a calibrated camera given known features (points or lines) in the world and their projections on the image. There are four minimal configurations using point and line features: 3 points, 2 points and 1 line, 1 point and 2 lines, 3 lines. The first and the last scenarios that depend solely on either points or lines have been studied a few decades earlier. However the mixed scenarios, which are more common in practice, have not been solved yet. In this paper we show that it is indeed possible to develop a general technique that can solve all four scenarios using the same approach and that the solutions involve computing the roots of either a 4th degree or an 8th degree equation. The centerpiece of our method is a simple and generic method that uses collinearity and coplanarity constraints for solving the pose. In addition to validating the performance of these algorithms in simulations, we also show a compelling application for geo-localization using image sequences and coarse (plane-based) 3D models of GPS challenged urban canyons.
Dynamics of gas flow in hollow core photonic bandgap fibers (2008)

The dynamics of gas flow in a hollow core photonic bandgap fiber is studied over four decades of pressure covering free molecular flow as well as hydrodynamic flow. Expressions are derived that allow for determination of the pressure inside the fiber as a function of time and position in the limits of Knudsen number Kn>>1 and Kn<<1. The expressions, which are validated by using absorption lines of acetylene as probes of the pressure inside the fiber, provide a straightforward way of predicting the temporal response for gas sensors of any fiber geometry.
Glycoprotein Hypersecretion Alters the Cell Wall in <i>Trichoderma reesei</i> Strains Expressing the <i>Saccharomyces cerevisiae</i> Dolichylphosphate Mannose Synthase Gene (2006)

Expression of the Saccharomyces cerevisiae DPM1 gene (coding for dolichylphosphate mannose synthase) in Trichoderma reesei (Hypocrea jecorina) increases the intensity of protein glycosylation and secretion and causes ultrastructural changes in the fungal cell wall. In the present work, we undertook further biochemical and morphological characterization of the DPM1-expressing T. reesei strains. We established that the carbohydrate composition of the fungal cell wall was altered with an increased amount of N-acetylglucosamine, suggesting an increase in chitin content. Calcofluor white staining followed by fluorescence microscopy indicated changes in chitin distribution. Moreover, we also observed a decreased concentration of mannose and alkali-soluble beta-(1,6) glucan. A comparison of protein secretion from protoplasts with that from mycelia showed that the cell wall created a barrier for secretion in the DPM1 transformants. We also discuss the relationships between the observed changes in the cell wall, increased protein glycosylation, and the greater secretory capacity of T. reesei strains expressing the yeast DPM1 gene.
A microwave beacon and guiding signals for airports and their approaches (2003)

A novel circular array of 90 equally spaced vertical dipoles is described. It is driven by just one element. When the spacing, length, and radius of the identical elements are properly chosen, the entire array is resonant with large currents in each element. These alternate 180/spl deg/ in phase and set up a far field that consists of 90 sharp spikes separated by 90 nulls. When dimensioned for a microwave frequency of 3 GHz, the array generates a field pattern that can be applied to provide a microwave beacon and guiding signals for aircraft approaching an airport. These would supplement the normal central high-intensity light and the approach lights, and provide a potentially useful guidance system for coastal airports embedded in dense fog that obscures the lights.
Mining a Cathepsin Inhibitor Library for New Antiparasitic Drug Leads (2011)

The targeting of parasite cysteine proteases with small molecules is emerging as a possible approach to treat tropical parasitic diseases such as sleeping sickness, Chagas' disease, and malaria. The homology of parasite cysteine proteases to the human cathepsins suggests that inhibitors originally developed for the latter may be a source of promising lead compounds for the former. We describe here the screening of a unique ∼ 2,100-member cathepsin inhibitor library against five parasite cysteine proteases thought to be relevant in tropical parasitic diseases. Compounds active against parasite enzymes were subsequently screened against cultured Plasmodium falciparum, Trypanosoma brucei brucei and/or Trypanosoma cruzi parasites and evaluated for cytotoxicity to mammalian cells. The end products of this effort include the identification of sub-micromolar cell-active leads as well as the elucidation of structure-activity trends that can guide further optimization efforts.
Color‐Tunable Luminescent Materials Based on Functional Polysiloxane and Lanthanide Ions (2011)

Abstract Color‐tunable luminescent materials based on α, ω‐diethyl malonate terminated polydimethylsiloxane (EP) and lanthanide ions are synthesized. The EP is synthesized from α, ω‐hydride terminated polysiloxane (HP) and diethyl allyl malonate via hydrosilylation reaction. The FT‐IR spectra, 1 H NMR, 13 C NMR, and GPC are used to characterize the structures of HP and EP. The obtained materials, which were synthesized by incorporating Eu 3+ (or Tb 3+ ) ions into EP, exhibit excellent narrow‐width red (or green) emissions. Moreover, when integrating heterometallic Eu 3+ /Tb 3+ ions into EP, the luminescence spectra of the complexes are composed of green and red emission. Color‐tunable luminescent materials are obtained by tuning the proportions of Eu 3+ and Tb 3+ ions. magnified image
Implementation of a telemonitoring system for the control of an EEG-based brain-computer interface (2003)

By the use of a brain-computer interface (BCI), it is possible for completely paralyzed patients, who have lost their ability to speak, to have a new possibility to communicate with their environment. The training with such a BCI system can be performed at the patient's home, if there is a responsible person present who is familiar with the system. This person has to adjust different parameters and to adapt the training individually to each patient. Since this function is usually taken over by the developers of the system, the number of patients who can be included in regular BCI training is restricted due to geographical distances. This paper describes the implementation of a telemonitoring system, which makes it possible for the developer to control and supervise the BCI training from his or her own place of work. First experiences with a patient living far away from the developer's lab are reported.
A New Subjective Audiovisual & Video Quality Testing Recommendation (2014)

This paper introduces ITU-T Recommendation P.913, a new subjective video quality testing standard approved in January 2014. This Recommendation focuses on the evaluation of flat screens, laptops, and mobile devices. P.913 emphasizes flexibility of environment, rating scale, display technology, and stimulus modality (video, audio, or audiovisual). To balance this flexibility, P.913 includes mandatory reporting requirements.
A distinct type of glycerol-3-phosphate acyltransferase with <i>sn</i> -2 preference and phosphatase activity producing 2-monoacylglycerol (2010)

The first step in assembly of membrane and storage glycerolipids is acylation of glycerol-3-phosphate (G3P). All previously characterized membrane-bound, eukaryotic G3P acyltransferases (GPATs) acylate the sn-1 position to produce lysophosphatidic acid (1-acyl-LPA). Cutin is a glycerolipid with omega-oxidized fatty acids and glycerol as integral components. It occurs as an extracellular polyester on the aerial surface of all plants, provides a barrier to pathogens and resistance to stress, and maintains organ identity. We have determined that Arabidopsis acyltransferases GPAT4 and GPAT6 required for cutin biosynthesis esterify acyl groups predominantly to the sn-2 position of G3P. In addition, these acyltransferases possess a phosphatase domain that results in sn-2 monoacylglycerol (2-MAG) rather than LPA as the major product. Such bifunctional activity has not been previously described in any organism. The possible roles of 2-MAGs as intermediates in cutin synthesis are discussed. GPAT5, which is essential for the accumulation of suberin aliphatics, also exhibits a strong preference for sn-2 acylation. However, phosphatase activity is absent and 2-acyl-LPA is the major product. Clearly, plant GPATs can catalyze more reactions than the sn-1 acylation by which they are currently categorized. Close homologs of GPAT4-6 are present in all land plants, but not in animals, fungi or microorganisms (including algae). Thus, these distinctive acyltransferases may have been important for evolution of extracellular glycerolipid polymers and adaptation of plants to a terrestrial environment. These results provide insight into the biosynthetic assembly of cutin and suberin, the two most abundant glycerolipid polymers in nature.
Cellulose-Derived Oligomers Act as Damage-Associated Molecular Patterns and Trigger Defense-Like Responses (2017)

The plant cell wall, often the site of initial encounters between plants and their microbial pathogens, is composed of a complex mixture of cellulose, hemicellulose, and pectin polysaccharides as well as proteins. The concept of damage-associated molecular patterns (DAMPs) was proposed to describe plant elicitors like oligogalacturonides (OGs), which can be derived by the breakdown of the pectin homogalacturon by pectinases. OGs act via many of the same signaling steps as pathogen- or microbe-associated molecular patterns (PAMPs) to elicit defenses and provide protection against pathogens. Given both the complexity of the plant cell wall and the fact that many pathogens secrete a wide range of cell wall-degrading enzymes, we reasoned that the breakdown products of other cell wall polymers may be similarly biologically active as elicitors and may help to reinforce the perception of danger by plant cells. Our results indicate that oligomers derived from cellulose are perceived as signal molecules in Arabidopsis (<i>Arabidopsis thaliana</i>), triggering a signaling cascade that shares some similarities to responses to well-known elicitors such as chitooligomers and OGs. However, in contrast to other known PAMPs/DAMPs, cellobiose stimulates neither detectable reactive oxygen species production nor callose deposition. Confirming our idea that both PAMPs and DAMPs are likely to cooccur at infection sites, cotreatments of cellobiose with flg22 or chitooligomers led to synergistic increases in gene expression. Thus, the perception of cellulose-derived oligomers may participate in cell wall integrity surveillance and represents an additional layer of signaling following plant cell wall breakdown during cell wall remodeling or pathogen attack.