gebhart/patrepeval huggingface dataset:
Bistable Injection Lasers (1983)

Semiconductor lasers with inhomogeneous current injection have been proposed nearly twenty years ago [1] as highly compact and efficient bistable devices. Recently, we demonstrated that a semiconductor laser with a segmented contact, as shown in Fig. 1, displays bistability without pulsations. The key to the proper design of such a bistable laser is the electrical isolation between the two segments which requires that the parasitic resistance (R p in Fig. 2) between the two contacts be as large as possible. This can be achieved by doping the top cladding layer only slightly p-type.
Effects of Hydrostatic Pressure and Transforming Growth Factor- <i>β</i> 3 on Adult Human Mesenchymal Stem Cell Chondrogenesis <i>In Vitro</i> (2006)

This study examined the effects of intermittent hydrostatic pressure (IHP) and transforming growth factor-beta 3 on chondrogenesis of adult human mesenchymal stem cells (hMSCs) in vitro. Chondrogenic gene expression was determined by quantifying mRNA signal levels for SOX9, a transcription factor critical for cartilage development and the cartilage matrix proteins, aggrecan and type II collagen. Extracellular matrix production was determined by weight and histology. IHP was applied to hMSCs in pellet culture at a level of 10 MPa and a frequency of 1 Hz for 4 h per day for periods of 3, 7, and 14 days. hMSCs responded to addition of TGF-beta 3 (10 ng/mL) with a greater than 10-fold increase (p < 0.01) in mRNA levels for each, SOX9, type II collagen, and aggrecan during a 14-day culture period. Applying IHP in the presence of TGF-beta 3 further increased the mRNA levels for these proteins by 1.9-, 3.3-, and 1.6-fold, respectively, by day 14. Chondrogenic mRNA levels were increased with just exposure to IHP. Extracellular matrix deposition of type II collagen and aggrecan increased in the pellets as a function of treatment conditions and time of culture. This study demonstrated adjunctive effects of IHP on TGF-beta 3-induced chondrogenesis and suggests that mechanical loading can facilitate articular cartilage tissue engineering.
Generation of Nucleic Acid Aptamer Candidates against a Novel Calicivirus Protein Target (2021)

Human norovirus is the leading cause of foodborne illness globally. One of the challenges in detecting noroviruses is the identification of a completely broadly reactive ligand; however, all detection ligands generated to date target the viral capsid, the outermost of which is the most variable region of the genome. The VPg is a protein covalently linked to the viral genome that is necessary for replication but hitherto remains underexplored as a target for detection or therapeutics. The purpose of this work was to generate nucleic acid aptamers against human norovirus (Norwalk) and cultivable surrogate (Tulane) VPgs for future use in detection and therapeutics. Eight rounds of positive-SELEX and two rounds of counter-SELEX were performed. Five and eight unique aptamer sequences were identified for Norwalk and Tulane VPg, respectively, all of which were predicted to be stable (∆G < -5.0) and one of which occurred in both pools. All candidates displayed binding to both Tulane and Norwalk VPg (positive:negative > 5.0), and all but two of the candidates displayed very strong binding (positive:negative > 10.0), significantly higher than binding to the negative control protein (<i>p</i> < 0.05). Overall, this work reports a number of aptamer candidates found to be broadly reactive and specific for in vitro-expressed VPgs across genus that could be used for future application in detection or therapeutics. Future work characterizing binding of the aptamer candidates against native VPgs and in therapeutic applications is needed to further evaluate their application.
Vaccine blunts fentanyl potency in male rhesus monkeys (2019)

Abstract One proposed factor contributing to the increased opioid overdose deaths is the increased frequency of synthetic opioids, including fentanyl and fentanyl analogs. A treatment strategy currently under development to address the ongoing opioid crisis is immunopharmacotherapies or opioid-targeted vaccines. The present study determined the effectiveness and selectivity of a fentanyl-tetanus toxoid conjugate vaccine to alter the behavioral effects of fentanyl and a structurally dissimilar mu-opioid agonist oxycodone in male rhesus monkeys (n=3-4). Fentanyl and oxycodone produced dose-dependent suppression of behavior in an assay of schedule-controlled responding and antinociception in an assay of thermal nociception (50°C). Acute naltrexone (0.032 mg/kg) produced an approximate 10-fold potency shift for fentanyl to decrease operant responding. The fentanyl vaccine was administered at weeks 0, 2, 4, 9, 19, and 44 and fentanyl or oxycodone potencies in both behavioral assays were redetermined over the course of 49 weeks. The vaccine significantly and selectively shifted fentanyl potency at least 10-fold in both assays at several time points over the entire experimental period. Mid-point titer levels were significantly correlated with fentanyl antinociceptive potency shifts. Antibody affinity for fentanyl as measured by a competitive binding assay increased over time to around 3-4 nM. The fentanyl vaccine also significantly increased fentanyl plasma levels approximately 6-fold consistent with the hypothesis that the vaccine sequesters fentanyl in the blood. Overall, these results support the continued development and evaluation of this fentanyl vaccine to address the ongoing opioid crisis. Highlights Vaccine blunted fentanyl rate-suppression potency ∼ 10-fold Vaccine blunted fentanyl antinociceptive potency ∼25-fold Fentanyl vaccine was as effective as acute 0.032 mg/kg naltrexone Vaccine was selective for fentanyl and not oxycodone Antibody immune response ∼ 3 nM affinity for fentanyl
Abstract 16013: Annexin-A1 Interacts With NF-KappaB Inhibiting Its Activation: Cardioprotection in vivo and in vitro (2011)

Background: Annexin-A1 (ANXA1), a mediator of the anti-inflammatory action of glucocorticoids, is cardioprotective in multiple preclinical models of myocardial ischemia-reperfusion (I/R), but under...
An inactivated multivalent influenza A virus vaccine is broadly protective in mice and ferrets (2022)

Influenza A viruses (IAVs) present major public health threats from annual seasonal epidemics and pandemics and from viruses adapted to a variety of animals including poultry, pigs, and horses. Vaccines that broadly protect against all such IAVs, so-called "universal" influenza vaccines, do not currently exist but are urgently needed. Here, we demonstrated that an inactivated, multivalent whole-virus vaccine, delivered intramuscularly or intranasally, was broadly protective against challenges with multiple IAV hemagglutinin and neuraminidase subtypes in both mice and ferrets. The vaccine is composed of four β-propiolactone-inactivated low-pathogenicity avian IAV subtypes of H1N9, H3N8, H5N1, and H7N3. Vaccinated mice and ferrets demonstrated substantial protection against a variety of IAVs, including the 1918 H1N1 strain, the highly pathogenic avian H5N8 strain, and H7N9. We also observed protection against challenge with antigenically variable and heterosubtypic avian, swine, and human viruses. Compared to control animals, vaccinated mice and ferrets demonstrated marked reductions in viral titers, lung pathology, and host inflammatory responses. This vaccine approach indicates the feasibility of eliciting broad, heterosubtypic IAV protection and identifies a promising candidate for influenza vaccine clinical development.
Lateral mode constrictions for broad-ridge quantum cascade lasers (2017)

We develop a method for analyzing the lateral modes of broad-ridge quantum cascade lasers (QCLs) that incorporate an intracavity lateral constriction. The calculations provide results that are in good agreement with the recent data taken on broad-ridge QCLs that incorporate such lateral constrictions. We conclude by providing design principles for broad-ridge QCLs that implement an intracavity lateral constriction.
Precision micromachining with pulsed green lasers (1998)

We have developed high-precision machining based on high-power pulsed green lasers (30–40 ns pulse width) at multi-kHz repetition rate. Dynamics of material removal has been investigated using a copper vapor laser. We found that noticeable surface evaporation starts to appear as laser intensity exceeds 107 W/cm2. Material removal is then dominated by ablation at higher laser intensities. However, strong plasma absorption starts to appear as laser intensity exceeds 2 GW/cm2. This prolongs material heating by hot plasma via electron conduction, resulting in noticeable melt formation and expulsion. Maintaining laser radiance below the plasma-ignition threshold minimizes this melt formation. The optimum rate of ablation on metals was found to be ∼1 μm/pulse with a laser fluence of 50 J/cm2. Higher material removal rate can be achieved at higher fluence, but is mostly accompanied with unwanted melt formation and ejection. By keeping laser intensity within a few GW/cm2, we have demonstrated high-aspect-ratio machining with micron-scale accuracy and negligible heat affected zone. High-quality percussion drilling, trepanning, grooving, and slotting were demonstrated on metals and ceramics with a negligible heat affected zone. Straight holes with sizes varying from 500 to less than 25 μm were consistently drilled with a height-to-diameter ratio up to 40. The high quality machining with scalable machining speed promises expanded use of pulsed green lasers in micromachining.
菲涅耳非相干关联全息图(综述) (2009)

Holographic imaging offers a reliable and fast method to capture the complete three-dimensional (3D) information of the scene from a single perspective. We review our recently proposed single-channel optical system for generating digital Fresnel holograms of 3D real-existing objects illuminated by incoherent light. In this motionless holographic technique, light is reflected, or emitted from a 3D object, propagates through a spatial light modulator (SLM), and is recorded by a digital camera. The SLM is used as a beamsplitter of the single-channel incoherent interferometer, such that each spherical beam originated from each object point is split into two spherical beams with two different curve radii. Incoherent sum of the entire interferences between all the couples of spherical beams creates the Fresnel hologram of the observed 3D object. When this hologram is reconstructed in the computer, the 3D properties of the object are revealed.
Construction of Recombinant Sindbis-Based Expression Vectors for the Study of HCV Genes and Their Products (2003)

There are currently no methods for propogating hepatitis C virus (HCV) in culture useful for the analysis of viral proteins. Therefore, we have utilized Sindbis virus-based vectors to express and study HCV genes and their products.
Efficacy of hepatitis B virus ribonuclease H inhibitors, a new class of replication antagonists, in FRG human liver chimeric mice (2017)

Chronic hepatitis B virus infection cannot be cured by current therapies, so new treatments are urgently needed. We recently identified novel inhibitors of the hepatitis B virus ribonuclease H that suppress viral replication in cell culture. Here, we employed immunodeficient FRG KO mice whose livers had been engrafted with primary human hepatocytes to ask whether ribonuclease H inhibitors can suppress hepatitis B virus replication in vivo. Humanized FRG KO mice infected with hepatitis B virus were treated for two weeks with the ribonuclease H inhibitors #110, an α-hydroxytropolone, and #208, an N-hydroxypyridinedione. Hepatitis B virus viral titers and S and e antigen plasma levels were measured. Treatment with #110 and #208 caused significant reductions in plasma viremia without affecting hepatitis B virus S or e antigen levels, and viral titers rebounded following treatment cessation. This is the expected pattern for inhibitors of viral DNA synthesis. Compound #208 suppressed viral titers of both hepatitis B virus genotype A and C isolates. These data indicate that Hepatitis B virus replication can be suppressed during infection in an animal by inhibiting the viral ribonuclease H, validating the ribonuclease H as a novel target for antiviral drug development.
Synthesis and characterization of graft copolymers from lignin and 2‐propenamide (1984)

Abstract Free radical polymerization of 2‐propenamide in the presence of lignin, anhydrous calcium chloride, and cericion in photolyzed dioxane solvent produces a reaction product containing lignin‐(1‐amidoethylene) graft copolymer. Up to 49 weight percent of the product is poly(1‐amidoethylene) homopolymer. Photolysis products of dioxane and the presence of anhydrous calcium chloride are critical to the reaction. A maximum yield of polymer is obtained when the dioxane solvent is irradiated for 3 h in a Pyrex vessel and the reaction mixture contains 2.0 weight percent calcium chloride. Aqueous size exclusion chromatography of reaction products shows that the molecular size of the lignin‐(2‐propenamide) reaction product is significantly greater than that of unreacted lignin, that the side chain and the lignin backbone migrate as one unit through the column, and that mixtures of lignin and poly(1‐amidoethylene) are easily separated by the chromatographic column.
Morphology-controlled copper nanowire synthesis and magnetic field assisted self-assembly (2018)

Morphology, dimensions, crystalline structure and compositions of nanomaterials are very critical in determining their unique characteristics. Here we report how the reducing agent concentration affects the surface morphology of copper nanowires and establish the optimized reaction conditions to synthesize high aspect ratio nanowires with a smooth surface. Also, reported is the magnetic field assisted technique to control the orientational and positional ordering of cupronickel nanowires (Cu/Ni NWs) on a large-scale area. A combination of magnetic field, surface derivatization and photolithographic techniques allowed self-assembly of Cu/Ni NWs into channels. The channel resistance as a function of the applied magnetic field during fabrication shows an anomalous decrease owing to the positional end-to-end alignment of NWs. Magnetic field and areal NW density dependence of NW sheet resistance in channels is presented and analyzed using scaling theoretical models.
Recognition of Multiple Classes of Hepatitis C Antibodies Increases Detection Sensitivity in Oral Fluid (2001)

Paired serum-oral fluid samples from 127 hepatitis C virus (HCV)-positive and 31 HCV-negative patients were tested for the presence of anti-HCV using the Ortho HCV 3.0 ELISA. Using the immunoglobulin G (IgG)-specific detection antibody provided with the HCV 3.0 ELISA we attained 100% sensitivity and specificity with serum samples; however, sensitivity in oral fluid samples was only 81%. By modifying the HCV 3.0 ELISA to utilize a secondary antibody cocktail that recognizes not only IgG but IgA and IgM as well, we attained 100% specificity and sensitivity with oral fluid samples.
Supervised label transfer for semantic segmentation of street scenes (2010)

In this paper, we propose a robust supervised label transfer method for the semantic segmentation of street scenes. Given an input image of street scene, we first find multiple image sets from the training database consisting of images with annotation, each of which can cover all semantic categories in the input image. Then, we establish dense correspondence between the input image and each found image sets with a proposed KNN-MRF matching scheme. It is followed by a matching correspondences classification that tries to reduce the number of semantically incorrect correspondences with trained matching correspondences classification models for different categories. With those matching correspondences classified as semantically correct correspondences, we infer the confidence values of each super pixel belonging to different semantic categories, and integrate them and spatial smoothness constraint in a markov random field to segment the input image. Experiments on three datasets show our method outperforms the traditional learning based methods and the previous nonparametric label transfer method, for the semantic segmentation of street scenes.
Small, Fast, and Under Control: Wireless Resonant Magnetic Micro-agents (2009)

Primary challenges in the building of untethered submillimeter sized robots include propulsion methods, power supply, and control. We present a novel type of microrobot called MagMite that utilizes a new class of wireless resonant magnetic micro-actuator that accomplishes all three tasks. The term MagMite is derived from Magnetic Mite —a tribute to the underlying magnetic propulsion principle and the micro-scale dimensions of the robot. The device harvests magnetic energy from the environment and effectively transforms it into inertia-and impact-driven mechanical force while being fully controllable. It can be powered and controlled with oscillating fields in the kilohertz range and strengths as low as 2 mT, which is only roughly 50 times the average Earth magnetic field. These microrobotic agents with dimensions less than 300 μm × 300 μm × 70 μm and a total mass of 30—50 μg are capable of moving forward, backward and turning in place while reaching controllable speeds in excess of 12.5 mm s —1 or 42 times the robot’s body length per second. The robots produce enough force to push micro-objects of similar sizes and can be visually servoed through a maze in a fully automated fashion. The prototype devices exhibit an overall degree of flexibility, controllability, and performance unmatched by other microrobots reported in the literature. The robustness of the MagMites leads to high experimental repeatability, which in turn enabled us to successfully compete in the RoboCup 2007 and 2009 Nanogram competitions. In this work it is demonstrated how the robots exhibit a plethora of driving behaviors, how they can operate on a host of unstructured surfaces under both dry and wet conditions, and how they can accomplish fully automated micromanipulation tasks. Various micro-objects ranging from beads to biological entities have been successfully manipulated. To the same end, multi-agent studies have shown great promise to be used in cooperative tasks.
Methods to array photonic crystal microcavities for high throughput high sensitivity biosensing on a silicon-chip based platform (2012)

We experimentally demonstrate a method to create large-scale chip-integrated photonic crystal sensor microarrays by combining the optical power splitting characteristics of multi-mode interference (MMI) power splitters and transmission drop resonance characteristics of multiple photonic crystal microcavities arrayed along the length of the same photonic crystal waveguide. L13 photonic crystal microcavities are employed which show high Q values (~9300) in the bio-ambient phosphate buffered saline (PBS) as well as high sensitivity, experimentally demonstrated to ~98 atto-grams. Two different probe antibodies were specifically detected simultaneously with a control sample, in the same experiment.
Coformulation of a Novel Human α -Galactosidase A With the Pharmacological Chaperone AT1001 Leads to Improved Substrate Reduction in Fabry Mice (2015)

Fabry disease is an X-linked lysosomal storage disorder caused by mutations in the gene that encodes α-galactosidase A and is characterized by pathological accumulation of globotriaosylceramide and globotriaosylsphingosine. Earlier, the authors demonstrated that oral coadministration of the pharmacological chaperone AT1001 (migalastat HCl; 1-deoxygalactonojirimycin HCl) prior to intravenous administration of enzyme replacement therapy improved the pharmacological properties of the enzyme. In this study, the authors investigated the effects of coformulating AT1001 with a proprietary recombinant human α-galactosidase A (ATB100) into a single intravenous formulation. AT1001 increased the physical stability and reduced aggregation of ATB100 at neutral pH in vitro, and increased the potency for ATB100-mediated globotriaosylceramide reduction in cultured Fabry fibroblasts. In Fabry mice, AT1001 coformulation increased the total exposure of active enzyme, and increased ATB100 levels in cardiomyocytes, cardiac vascular endothelial cells, renal distal tubular epithelial cells, and glomerular cells, cell types that do not show substantial uptake with enzyme replacement therapy alone. Notably, AT1001 coformulation also leads to greater tissue globotriaosylceramide reduction when compared with ATB100 alone, which was positively correlated with reductions in plasma globotriaosylsphingosine. Collectively, these data indicate that intravenous administration of ATB100 coformulated with AT1001 may provide an improved therapy for Fabry disease and thus warrants further investigation.
An Empirical Evaluation of a WYSIWYT Testing Methodology for Spreadsheets (1999)

Spreadsheets languages, which include commercial spreadsheet systems as well as various research systems, are perhaps the most widely-used type of domain-specific programming language. They are unusual programming languages in that they are aimed primarily at a non-traditional audience, namely end users, although other classes of users write programs (spreadsheets) in them as well. Despite the large amount of software created in these languages, the issue of how to test spreadsheets has been largely ignored. In earlier work, we presented a methodology for testing spreadsheets. In this paper, we investigate whether spreadsheet programmers assisted by the methodology are more effective at testing spreadsheets than those who perform only ad hoc testing.
Continuous Flow Total Artificial Heart: Modeling and Feedback Control in a Mock Circulatory System (2008)

We developed a mock circulatory loop and used mathematical modeling to test the in vitro performance of a physiologic flow control system for a total artificial heart (TAH). The TAH was constructed from two continuous flow pumps. The objective of the control system was to maintain loop flow constant in response to changes in outflow resistance of either pump. Baseline outflow resistances of the right (pulmonary vascular resistance) and the left (systemic vascular resistance) pumps were set at 2 and 18 Wood units, respectively. The corresponding circuit flow was 4 L/min. The control system consisted of two digital integral controllers, each regulating the voltage, hence, the rotational speed of one of the pumps. The in vitro performance of the flow control system was validated by increasing systemic and pulmonary vascular resistances in the mock loop by 4 and 8 Wood units (simulating systemic and pulmonary hypertension conditions), respectively. For these simulated hypertensive states, the flow controllers regulated circuit flow back to 4 L/min within seconds by automatically adjusting the rotational speed of either or both pumps. We conclude that this multivariable feedback mechanism may constitute an adequate supplement to the inherent pressure sensitivity of rotary blood pumps for the automatic flow control and left-right flow balance of a dual continuous flow pump TAH system.
Optical Time Slice Switching (OTSS): An All-Optical Sub-Wavelength Solution Based on Time Synchronization (2013)

We propose an all optical time slice switching (OTSS) architecture. With time synchronization and repetitive time slices structure, OTSS can obtain global available time slots information and realize contentionless sub-wavelength switching without optical buffers.