gebhart/patrepeval huggingface dataset:
Small Molecule Inhibitors of BAF; A Promising Family of Compounds in HIV-1 Latency Reversal (2015)

Persistence of latently infected cells in presence of Anti-Retroviral Therapy presents the main obstacle to HIV-1 eradication. Much effort is thus placed on identification of compounds capable of HIV-1 latency reversal in order to render infected cells susceptible to viral cytopathic effects and immune clearance. We identified the BAF chromatin remodeling complex as a key player required for maintenance of HIV-1 latency, highlighting its potential as a molecular target for inhibition in latency reversal. Here, we screened a recently identified panel of small molecule inhibitors of BAF (BAFi's) for potential to activate latent HIV-1. Latency reversal was strongly induced by BAFi's Caffeic Acid Phenethyl Ester and Pyrimethamine, two molecules previously characterized for clinical application. BAFi's reversed HIV-1 latency in cell line based latency models, in two ex vivo infected primary cell models of latency, as well as in HIV-1 infected patient's CD4 + T cells, without inducing T cell proliferation or activation. BAFi-induced HIV-1 latency reversal was synergistically enhanced upon PKC pathway activation and HDAC-inhibition. Therefore BAFi's constitute a promising family of molecules for inclusion in therapeutic combinatorial HIV-1 latency reversal.
Elimination of potassium channel expression by antisense oligonucleotides in a pituitary cell line. (1995)

The clonal rat pituitary cell line GH4C1 expresses the genes for several voltage-dependent potassium channels including Kv1.5 and Kv1.4. Dexamethasone, a glucocorticoid agonist, induces a slowly inactivating potassium current in these cells but does not alter the amplitude of a rapidly inactivating component of potassium current. We have found that the induction of the slowly inactivating current can be blocked by an antisense phosphorothioate deoxyoligonucleotide to the Kv1.5 mRNA sequence. In contrast, antisense deoxyoligonucleotides against Kv1.4 mRNA specifically decrease the expression of the dexamethasone-insensitive rapidly inactivating current. These results demonstrate the usefulness of antisense oligonucleotides in correlating potassium currents with specific potassium channel proteins in the cell types in which they are naturally expressed.
Deodorization and finished oil handling (1981)

Abstract Deodorization is the final processing step in the production of edible oil products. Chemical/physical aspects of deodorization and mechanical design of deodorizers are explained, including chemical analysis of feedstock, finished product and by‐products. This includes normal operating standards and means of correcting off specification operation. Operating procedures, labor requirements and maintenance are reviewed. Finished oil handling includes use of antioxidants, inert gas addition, pipelines and storage facilities. Emphasis is on the practical operations of deodorization and finished soybean oil handling.
Super-charged NK cells inhibit growth and progression of stem-like/poorly differentiated oral tumors<i>in vivo</i>in humanized BLT mice; effect on tumor differentiation and response to chemotherapeutic drugs (2018)

Therapeutic role of NK cells in solid tumors was challenged previously even though their role in hematological malignancies has clearly been established. Furthermore, functions and numbers of NK cells are greatly suppressed in oral cancer patients necessitating effective future NK immunotherapeutic strategies to aid in the control of disease. The humanized-BLT (hu-BLT) mice were used to implant stem-like/undifferentiated oral tumors to study the role of super-charged NK cells with and without feeding with AJ2 probiotic bacteria. Implanted CSC/undifferentiated tumors resected from NK-injected mice exhibited differentiated phenotype, grew slowly, and did not cause weight loss, whereas those from tumor-bearing mice without NK-injection remained relatively more stem-like/poorly-differentiated, grew faster, and caused significant weight loss. Moreover, <i>in vitro</i> NK-differentiated tumors were sensitive to chemotherapeutic drugs, and when implanted in the oral-cavity grew no or very small tumors in mice. When NK-mediated differentiation of tumors was blocked by IFN-γ and TNF-α antibodies before implantation, tumors grew rapidly, remained stem-like/poorly-differentiated and became resistant to chemotherapeutic drugs. Loss of NK cytotoxicity and decreased IFN-γ secretion in tumor-bearing mice in PBMCs, splenocytes, bone marrow derived immune cells and enriched NK cells was restored by the injection of super-charged NK cells with or without feeding with AJ2. Much greater infiltration of CD45<sup>+</sup> and T cells were observed in tumors resected from the mice, along with the restored secretion of IFN-γ from purified T cells from splenocytes in NK-injected tumor-bearing mice fed with AJ2 probiotic bacteria. Thus, super-charged NK cells prevent tumor growth by restoring effector function resulting in differentiation of CSCs/undifferentiated-tumors in hu-BLT mice.
MicroRNA-182 downregulates metastasis suppressor 1 and contributes to metastasis of hepatocellular carcinoma (2012)

miR-182 could promote metastasis of HCC and inhibit the expression of MTSS1. miR-182 and MTSS1 are potential prognostic markers and/or therapeutic targets in HCC.
The histogram feature - a resource-efficient Weak Classifier (2008)

This paper presents a Weak Classifier that is extremely fast to compute, yet highly discriminant. This Weak Classifier may be used in, for example, a boosting framework and is the result of a novel way of organizing and evaluating Histograms of Oriented Gradients. The method requires only one access to main memory to evaluate each feature, in comparison with the more well-known Haar features which require somewhere between six and nine memory accesses to evaluate each feature. This low memory bandwidth makes the Weak Classifier especially ideal for use in small systems with little or no memory cache available. The presented Weak Classifier has been extensively tested in a boosted framework on data sets consisting of pedestrians and various road signs. The classifier yields detection results that are far superior than the results obtained from Haar features when tested on road signs and similar structures, whereas the detection results are comparable to those of Haar features when tested on pedestrians. In addition, the computational resources necessary for these results have been shown to be considerably smaller for the new weak classifier.
Silibinin Regulates Lipid Metabolism and Differentiation in Functional Human Adipocytes (2016)

Silibinin, a natural plant flavonolignan is the main active constituent found in milk thistle (Silybum marianum). It is known to have hepatoprotective, anti-neoplastic effect, and suppresses lipid accumulation in adipocytes. Objective of this study was to investigate the effect of silibinin on adipogenic differentiation and thermogenic capacity of human adipose tissue derived mesenchymal stem cells. Silibinin (10 μM) treatment, either at the beginning or at the end of adipogenic differentiation, resulted in an increase of SIRT-1, PPARα, Pgc-1α, and UCPs gene expression. Moreover, silibinin administration resulted in a decrease of PPARγ, FABP4, FAS, and MEST/PEG1 gene expression during the differentiation, confirming that this compound is able to reduce fatty acid accumulation and adipocyte size. Our data showed that silibinin regulated adipocyte lipid metabolism, inducing thermogenesis and promoting a brown remodeling in adipocyte. Taken together, our findings suggest that silibinin increases UCPs expression by stimulation of SIRT1, PPARα, and Pgc-1α, improved metabolic parameters, decreased lipid mass leading to the formation of functional adipocytes.
Eye-Gaze and Augmented Reality Framework for Driver Assistance (2013)

Driver inattention or cognitive overload is among the leading contributor to road accidents. Driver behavioral cues could be employed in advanced driver assistance systems (ADASs) to alleviate such accidents. Facial and eye gaze are among the most import behavioral cues to reflect driver active state within the vehicle. With the intent to improve the human error related accidental controls in mind, a simple and smart ADAS is proposed that could assist the driver based on continues monitoring of facial and eye gaze information. The system measures driver eye gaze within near frontal facial positions and project future position of vehicle on windscreen based on the vehicle parameters and driver's active eye gaze estimates, assuming the movement of vehicle in a straight line. The projections adapt to shift in driver's perspective. The projection over the wind screen give visualization as if the lines are physically drawn over road according to the width of the vehicle. The system also warns the driver when there is constant shift in head or eye gaze from normal forward facing positions, beyond some threshold period. The system is based on live video input from low-end webcam. The system will reinforce driver ability in effective estimation of future positions of vehicle and leverage better control while driving. In addition it can also be employed to assist novice drivers to keep track of the width of vehicle during the training sessions.
Probing the Multidomain Structure of the Type I Regulatory Subunit of cAMP-Dependent Protein Kinase Using Mutational Analysis:  Role and Environment of Endogenous Tryptophans (2000)

The regulatory R-subunit of cAMP-dependent protein kinase (cAPK) is a thermostable multidomain protein. It contains a dimerization domain at the N-terminus followed by an inhibitor site that binds the catalytic C-subunit and two tandem cAMP-binding domains (A and B). Two of the three tryptophans in the RIalpha subunit, Trp188 and Trp222, lie in cAMP-binding domain A while Trp260 lies at the junction between domains A and B. The unfolding of wild-type RIalpha (wt-RI), monitored by intrinsic fluorescence, was described previously [Leon, D. A., Dostmann, W. R. G., and Taylor, S. S. (1991) Biochemistry 30, 3035 (1)]. To determine the environment of each tryptophan and the role of the adjacent domain in folding and stabilization of domain A, three point mutations, W188Y, W222Y, and W260Y, were introduced. The secondary structure of wt-RI and the point mutants has been studied by far-UV circular dichroism spectropolarimetry (CD). The CD spectra of wt-RI and the three point mutants are practically identical, and the thermal unfolding behavior is very similar. Intrinsic fluorescence and iodide quenching in the presence of increasing urea established that: (a) Trp222 is the most buried, whereas Trp188 is the most exposed to solvent; (b) Trp260 accounts for the quenching of fluorescence when cAMP is bound; and (c) Trp222 contributes most to the intrinsic fluorescence of the wt-RI-subunit, while Trp188 contributes least. For wt-RI, rR(W188Y), and rR(W260Y), removal of cAMP causes a destabilization, while excess cAMP stabilizes these three proteins. In contrast, rR(W222Y) was not stabilized by excess cAMP.
A run-time deadlock detector for concurrent Java programs (2005)

Deadlock is one of the most serious and complex problems concerning the reliability of concurrent Java programs. In this paper, we discuss how to dynamically detect deadlocks in concurrent Java programs at run-time, and propose a representation of synchronization waiting state in an execution of a Java program, which is named the Java thread-wait-for graph. We explicitly define all types of deadlocks in Java with this representation and present an algorithm to detect the deadlocks. Two implementation methods are also presented. We succeeded in detecting a deadlock from a concurrent program, including all types of synchronization waiting relations, using our tool.
Genome Engineering in Cyanobacteria: Where We Are and Where We Need To Go (2015)

Genome engineering of cyanobacteria is a promising area of development in order to produce fuels, feedstocks, and value-added chemicals in a sustainable way. Unfortunately, the current state of genome engineering tools for cyanobacteria lags far behind those of model organisms such as Escherichia coli and Saccharomyces cerevisiae. In this review, we present the current state of synthetic biology tools for genome engineering efforts in the most widely used cyanobacteria strains and areas that need concerted research efforts to improve tool development. Cyanobacteria pose unique challenges to genome engineering efforts because their cellular biology differs significantly from other eubacteria; therefore, tools developed for other genera are not directly transferrable. Standardized parts, such as promoters and ribosome binding sites, which control gene expression, require characterization in cyanobacteria in order to have fully predictable results. The application of these tools to genome engineering efforts is also discussed; the ability to do genome-wide searching and to introduce multiple mutations simultaneously is an area that needs additional research in order to enable fast and efficient strain engineering.
Tracking in Anatomic Pathology (2013)

Bar code-based tracking solutions, long present in clinical pathology laboratories, have recently made an appearance in anatomic pathology (AP) laboratories. Tracking of AP "assets" (specimens, blocks, slides) can enhance laboratory efficiency, promote patient safety, and improve patient care. Routing of excess clinical material into research laboratories and biorepositories are other avenues that can benefit from tracking of AP assets. Implementing tracking is not as simple as installing software and turning it on. Not all tracking solutions are alike. Careful analysis of laboratory workflow is needed before implementing tracking to assure that this solution will meet the needs of the laboratory. Such analysis will likely uncover practices that may need to be modified before a tracking system can be deployed. Costs that go beyond simply that of purchasing software will be incurred and need to be considered in the budgeting process. Finally, people, not technology, are the key to assuring quality. Tracking will require significant changes in workflow and an overall change in the culture of the laboratory. Preparation, training, buy-in, and accountability of the people involved are crucial to the success of this process. This article reviews the benefits, available technology, underlying principles, and implementation of tracking solutions for the AP and research laboratory.
Biodiesel preparation from Jatropha oil catalyzed by KF/Red mud catalyst (2016)

Biodiesel preparation from Jatropha oil catalyzed by KF/Red mud (KF/RM) was studied. The optimum values of parameters for preparation of Jatropha oil biodiesel were obtained. The conversion rate of transesterification reached 92.2% under the optimum conditions, and the used KF/RM could be regenerated. Catalyst characterization showed that KOH and KFeF4 were produced in KF/RM catalyst, which was crucial for the transesterification of Jatropha oil with methanol. Red mud was a good support to prepare KF-loaded catalyst, and prepared KF/RM was an excellent catalyst for biodiesel synthesis from Jatropha oil via transesterification reaction.
Inhibiton of browning by sulfur amino acids. 2. Fruit juices and protein-containing foods (1990)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTInhibiton of browning by sulfur amino acids. 2. Fruit juices and protein-containing foodsIbolya Molnar-Perl and Mendel FriedmanCite this: J. Agric. Food Chem. 1990, 38, 8, 1648–1651Publication Date (Print):August 1, 1990Publication History Published online1 May 2002Published inissue 1 August 1990https://pubs.acs.org/doi/10.1021/jf00098a005https://doi.org/10.1021/jf00098a005research-articleACS PublicationsRequest reuse permissionsArticle Views495Altmetric-Citations48LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Manuel Moreno Fraginals<A NAME="top1"></A>: uma historiografia marxista contra o dogmatismo (1989)

DOAJ is a unique and extensive index of diverse open access journals from around the world, driven by a growing community, committed to ensuring quality content is freely available online for everyone.
A Semiotics Analysis of the Web Series ‘DARK’ [Season:1 (2017)] (2023)

The visual arts speak to everyone. Throughout history, artists have used their artwork and paintings to convey stories. Some of the tales are given exactly, whereas others have been illustrated with artwork on walls, fabrics, papers. The realm of art has seen a variety of alterations and transformations during this period. The meaning of web series work in narrative is frequently enhanced by the utilisation of other creative forms. This study will look into the significance of mis en scene in the usage of visual storytelling in web series. This research of semiotic analysis uses the German web series “DARK [season-1 (2017)]- by Baran odar and Jantje Friese’s” as a sample. The German web series DARK [season-1 (2017)] uses the qualitative methodology to analyse the signifier and signified representation semiotics. The science fiction elements employed in the German online series DARK (season 1, 2017) relate to occurrences in real life as well as the sociological and psychological context. The analysis’s findings demonstrated that Saussure’s semiotic theory could be used to interpret these signs’ meanings in the Mise en scene. The results of the semiotic analysis will enable us to comprehend the importance of Mise en scene in web series and how it is utilised in visual storytelling. How to cite this article:Ganesh PNS. A Semiotics Analysis of the Web Series ‘DARK’ [Season:1 (2017)]. J Adv Res Jrnl Mass Comm 2023; 10(1): 1-7. DOI: https://doi.org/10.24321/2395.3810.202301
A Review on Human Body Communication: Signal Propagation Model, Communication Performance, and Experimental Issues (2017)

Human body communication (HBC), which uses the human body tissue as the transmission medium to transmit health informatics, serves as a promising physical layer solution for the body area network (BAN). The human centric nature of HBC offers an innovative method to transfer the healthcare data, whose transmission requires low interference and reliable data link. Therefore, the deployment of HBC system obtaining good communication performance is required. In this regard, a tutorial review on the important issues related to HBC data transmission such as signal propagation model, channel characteristics, communication performance, and experimental considerations is conducted. In this work, the development of HBC and its first attempts are firstly reviewed. Then a survey on the signal propagation models is introduced. Based on these models, the channel characteristics are summarized; the communication performance and selection of transmission parameters are also investigated. Moreover, the experimental issues, such as electrodes and grounding strategies, are also discussed. Finally, the recommended future studies are provided.
Market-based recommendation: Agents that compete for consumer attention (2004)

The amount of attention space available for recommending suppliers to consumers on e-commerce sites is typically limited. We present a competitive distributed recommendation mechanism based on adaptive software agents for efficiently allocating the 'consumer attention space', or banners. In the example of an electronic shopping mall, the task is delegated to the individual shops, each of which evaluates the information that is available about the consumer and his or her interests (e.g. keywords, product queries, and available parts of a profile). Shops make a monetary bid in an auction where a limited amount of 'consumer attention space' for the arriving consumer is sold. Each shop is represented by a software agent that bids for each consumer. This allows shops to rapidly adapt their bidding strategy to focus on consumers interested in their offerings. For various basic and simple models for on-line consumers, shops, and profiles, we demonstrate the feasibility of our system by evolutionary simulations as in the field of agent-based computational economics (ACE). We also develop adaptive software agents that learn bidding strategies, based on neural networks and strategy exploration heuristics. Furthermore, we address the commercial and technological advantages of this distributed market-based approach. The mechanism we describe is not limited to the example of the electronic shopping mall, but can easily be extended to other domains.
The role of mRNA stability and transcription in 0<sup>6</sup>-methylguanine DNA methyltransferase (MGMT) expression in Mer<sup>+</sup> human tumor cells (1995)

We have recently reported that following depletion of O6-methylguanine DNA methyltransferase (MGMT) activity by acute streptozotocin (STZ) treatment to sensitize innately chloroethylnitrosourea (CENU)-resistant HT-29 cells, the eventual repletion of activity occurs with no concommitant alterations in steady-state MGMT mRNA levels. This suggestion of a potentially stable transcript prompted studies to define the relative contributions of MGMT mRNA stability and transcription to cellular MGMT expression. Northern analysis of MGMT mRNA in actinomycin D-treated HT-29, MR-1 and A2182 cells, ranging in relative MGMT expression from high to low respectively, demonstrates relatively long MGMT mRNA half-lives of > 10-12 h. Cell lines with low and moderate levels of MGMT mRNA appear to have longer mRNA half-lives than those with high levels. Run-on transcription in nuclei isolated from cells with low to moderate MGMT mRNA levels demonstrates undetectable basal MGMT transcription rates. Collectively these data suggest that a very low transcription rate, coupled with a stable mRNA molecule, might result in the translation of pre-existing mRNA molecules. This translation may be responsible for the gradual recovery of MGMT and CENU resistance over 24 h following MGMT depletion.