gebhart/patrepeval huggingface dataset:
Real-time measurements of the gas-phase adsorption of n-alkylthiol mono- and multilayers on gold (1991)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTReal-time measurements of the gas-phase adsorption of n-alkylthiol mono- and multilayers on goldRoss C. Thomas, Li Sun, Richard M. Crooks, and Antonio J. RiccoCite this: Langmuir 1991, 7, 4, 620–622Publication Date (Print):April 1, 1991Publication History Published online1 May 2002Published inissue 1 April 1991https://pubs.acs.org/doi/10.1021/la00052a004https://doi.org/10.1021/la00052a004research-articleACS PublicationsRequest reuse permissionsArticle Views520Altmetric-Citations124LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Direct Attachment of Oligonucleotides to Quantum Dot Interfaces (2011)

A straightforward functionalization strategy for the direct attachment of single-stranded oligonucleotides (ssDNA) to quantum dot (qdot) interfaces is described. The approach takes advantage of a histidine-mediated phase transfer protocol that results in qdots with high colloidal stability in aqueous buffers. The weakly bound histidine encapsulation facilitates monolayer exchanged with both thiolated ssDNA and polyhistidine-tagged proteins. The successful biomodification at the qdot interface was probed by FRET analysis. The modest FRET efficiencies measured suggest the DNA to be in an extended conformation that is the result of high surface coverage that the direct attachment provides.
Angiogenic sprouting is regulated by endothelial cell expression of Slug (Snai2) (2014)

The Snail family of zinc-finger transcription factors are evolutionarily conserved proteins that control processes requiring cell movement. Specifically, they regulate epithelial-to-mesenchymal transitions (EMT) where an epithelial cell severs intercellular junctions, degrades basement membrane and becomes a migratory, mesenchymal-like cell. Interestingly, Slug expression has been observed in angiogenic endothelial cells (EC) in vivo, suggesting that angiogenic sprouting may share common attributes with EMT. Here, we demonstrate that sprouting EC in vitro express both Slug and Snail, and that siRNA-mediated knockdown of either inhibits sprouting and migration in multiple in vitro angiogenesis assays. We find that expression of MT1-MMP, but not of VE-Cadherin, is regulated by Slug and that loss of sprouting as a consequence of reduced Slug expression can be reversed by lentiviral-mediated re-expression of MT1-MMP. Activity of MMP2 and MMP9 are also affected by Slug expression, likely through MT1-MMP. Importantly, we find enhanced expression of Slug in EC in human colorectal cancer samples compared with normal colon tissue, suggesting a role for Slug in pathological angiogenesis. In summary, these data implicate Slug as an important regulator of sprouting angiogenesis, particularly in pathological settings.
The<i>O</i>-Carbamoyl-Transferase Alb15 Is Responsible for the Modification of Albicidin (2016)

Albicidin is a potent antibiotic and phytotoxin produced by Xanthomonas albilineans which targets the plant and bacterial DNA gyrase. We now report on a new albicidin derivative which is carbamoylated at the N-terminal coumaric acid by the action of the ATP-dependent O-carbamoyltransferase Alb15, present in the albicidin (alb) gene cluster. Carbamoyl-albicidin was characterized by tandem mass spectrometry from cultures of a Xanthomonas overproducer strain and the gene function confirmed by gene inactivation of alb15 in X. albilineans. Expression of alb15 in Escherichia coli and in vitro reconstitution of the carbamoyltransferase activity confirmed albicidin as the substrate. The chemical synthesis of carbamoyl-albicidin finally enabled us to assess its bioactivity by means of in vitro gyrase inhibition and antibacterial assays. Compared to albicidin, carbamoyl-albicidin showed a significantly higher inhibitory efficiency against bacterial gyrase (∼8 vs 49 nM), which identifies the carbamoyl group as an important structural feature of albicidin maturation.
Radioimmunoassay for the determination of pholedrine. (1997)

A radioimmunoassay for the determination of free pholedrine at the lower nanogram level was developed. The sulfate conjugate cross-reacts and has to be separated before the assay procedure in plasma.
Recycling processes and products in nylon 6 fibre industry (1990)

During the manufacture of nylon 6 polymer, about 8-10% of the caprolactam and its oligomers get converted into extractibles in the chips and during the manufacture of nylon 6 fibre, about 5-1 2% wastes are generated in solid form. It is essential that to make the production process es economically more vi­ able, both th e extractibles and the solid waste are converted into reusable products. This paper address es this problem and describes the recycling processes and the products obtained therefrom.
Protein design using model synthetic peptides. (1992)

We have designed and synthesized a small, unique protein molecule with defined secondary, tertiary and quaternary structure. This 35-residue peptide, containing a cysteine residue at its N-terminal end, was oxidized to form a 70-residue disulfide-linked two-stranded alpha-helical coiled-coil with the two alpha-helices parallel and in-register. The major contribution to the formation and stabilization of the alpha-helical coiled-coil is hydrophobic interactions between positions 2 and 5 of the heptapeptide repeat (Lys-Leu-Glu-Ala-Leu-Glu-Gly). The protein (L-protein) contains nine leucine-leucine hydrophobic interactions between the alpha-helices of the coiled-coil. Circular dichroism studies demonstrated that this protein in its reduced ([L (r)] or oxidized (L (o)] state was essentially 100% alpha-helical ([theta]220 = -34,050 and -32,000 degrees respectively) at pH 2 (0.1% aqueous trifluoroacetic acid). Our objective was to modify systematically the structure of L to delineate the contribution that various amino acid side chains make to the formation and stabilization of its three-dimensional structure. A-protein, which contains alanine instead of leucine at positions 16 and 19 of the hydrophobic repeat in each chain of the coiled-coil, was compared to the L-protein. At pH 2, the oxidized form of the A-protein [A (o)] was essentially 100% helical. However, the protein was much less stable to temperature denaturation compared to the L-protein. The replacement of two leucine-leucine interactions by two alanine-alanine interactions has a dramatic effect on the formation and stability of the two-stranded alpha-helical coiled-coil structure. The results of this study clearly demonstrate the validity of this synthetic model protein approach to understanding the molecular aspects responsible for the folding and stabilization of protein molecules.
A Novel Technique for Robust and Fast Segmentation of Corneal Layer Interfaces Based on Spectral-Domain Optical Coherence Tomography Imaging (2017)

A novel approach to segment corneal layer interfaces using optical coherence tomography images is presented. In this paper, we performed customized edge detection for initial location of interfaces, fitting the initial interfaces to circles via customized Hough transform, and refining interfaces by employing Kalman filtering to model the horizontal projectile motion of interface boundaries. Validation based on 20 B-scan images from 60 volumes shows that three layer interfaces in each image can be segmented within 0.52 s with an average absolute layer interface error below <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$5.4~\mu \text{m}$ </tex-math></inline-formula> . Compared with an existing method, we are able to yield significantly better or similar accuracy at a higher speed with inferior software environment. From the validation experiments based on images from normal human subjects, images with keratoconus and images with laser <italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">in situ</i> keratomileusis flap, we showed that the proposed customized Hough transform for circles can represent the corneal layer interfaces more accurately. On the other hand, Kalman filtering can handle the heavy noise exhibited in the image, and can be adapted to shape variation in order to be closer to the real-layer interfaces. In conclusion, our approach can be a potential tool to quantify corneal layer interfaces in a clinical environment with lower computational expenses while maintaining high effectiveness.
Paper microfluidic extraction and direct smartphone-based identification of pathogenic nucleic acids from field and clinical samples (2014)

A rapid, paper microfluidic- and smartphone-based protocol was developed for the extraction and direct fluorescent identification of the nucleic acids of Salmonella Typhimurium from field and clinical samples. Initially, liquid samples (10% diluted) from fresh poultry packaging were loaded on the paper chips and were lysed with Tris–EDTA (TE) buffer. Nucleic acids from the lysed samples were eluted through the paper channel with TE buffer and the paper channel was excised into three pieces for the further polymerase chain reaction (PCR) assay. The extraction efficiency was determined by measuring fluorescence reflectance with either a benchtop optical detection system (consisting of an LED light source, a pair of optical fibers, and a miniature spectrophotometer, all built on micro-positioning stages) or a smartphone-based fluorescent microscope (in-house fabricated). The limit of detection of Salmonella Typhimurium in 10% poultry packaging liquid with cellulose paper was 103 CFU mL−1, while that extracted with nitrocellulose paper was 104 CFU mL−1 (as determined by both PCR and fluorescence reflectance). Cellulose channels proved more appropriate for measuring low and very high concentrations of pathogen DNA, while nitrocellulose proved better for analysing the mid-range concentrations. We observed that DNA migrated through nitrocellulose at a faster rate and further than through cellulose due to charge–charge repulsion between nitrocellulose and DNA (both negatively charged), thus contributing to consistent and efficient extraction. We tested the efficiency of Salmonella extraction from 10% poultry packaging liquid, 10% whole blood, and 10% fecal samples, and obtained comparable extraction efficiency, as confirmed by smartphone-based direct fluorescent detection. This protocol is suitable for the direct detection of total bacteria count in a dirty sample (when specificity is not necessary) as well as determining extraction efficiency. This protocol is compatible with PCR, to provide specific information about the type of pathogen present in sample.
SmartNode: achieving 802.11 MAC interoperability in power-efficient ad hoc networks with dynamic range adjustments (2004)

The standard CSMA/CA based IEEE 802.11 protocol assumes that each node uses a certain fixed (or maximum) transmission power for the transmission of each packet. However, a MAC protocol with power adjustments can have significant benefits towards better power conservation and higher system throughput through better spatial reuse of spectrum. In this work, we propose a power efficient MAC layer algorithm, SmartNode, that is compatible with the basic RTS-CTS-DATA-ACK MAC protocol defined in IEEE 802.11. Our algorithm uses the minimum required power level for the transmission of data packets, which requires special handling for the exchange of control packets. Compared with previously proposed power-controlled MAC protocols, our algorithm does not require multiple data channels at the physical layer so that it is able to inter-operate with regular nodes running the existing IEEE 802.11 MAC protocol. Through extensive performance evaluations, we have demonstrated that our proposed algorithm is effective in a power-controlled ad hoc network - it is able to increase system throughput while conserving power with dynamic power adjustments.
A hypercube algorithm for GVT computation and its application in optimistic parallel simulation (2002)

We present an algorithm for computing the global virtual time (GVT) in an optimistic parallel discrete event simulation, on the distributed memory hypercube architecture. Our algorithm uses only 3N messages and runs in O(log N) time where N is the number of logical processors (LP's) representing components of the simulation system. It is based on the construction of a spanning binomial tree in the hypercube. In most simulation systems, there is an LP designated for GVT computation, called the GVT manager. Failure of the physical processor running this LP causes the simulation process to stop, and in such a case reorganization of LPs is necessary so that another logical processor take the roll of the GVT manager. In our algorithm, any LP in the system can elect itself to be the GVT manager and hence such reorganization is not necessary. We show how our algorithm can be used for memory management and hierarchical load balancing in a hypercube machine, and suggest a new technique to handle transient messages.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
A Critical Review of Polybenzimidazoles (1997)

Abstract About 8 years ago, Brinker and Robinson [l] invented the first aliphatic polybenzimidazoles. Two years later, aromatic polybenzimidazoles with impressive thermal properties were synthesized by Vogel and Marvel [2, 3] at the University of Illinois and later at Du Pont. Since then, high performance polybenzimidazoles received a great deal of attention by academia, U. S. government, and industry. Various new polybenzimidazoles have been invented, as reviewed in Refs. 4–12. Hoechst Celanese commercialized polybenzimidazole fiber for thermal protective clothing and fireblocking applications in 1983. In the mid 1980s, both Hoechst Celanese and Alpha Performance Company developed the technology to mold polyben- zimidazole parts (under the trade name of Celazole®). Alpha took over Celazole® technology completely in 1995 and continued marketing polybenzimidazole molded parts as sealing elements in high-temperature corrosive environments. Poly (2,2′-(m-phenylene)-5,5′ bibenzimidazole) is their product composition and is referred to as PBI.
Fast generation of realistic virtual humans (2017)

In this paper we present a complete pipeline to create ready-to-animate virtual humans by fitting a template character to a point set obtained by scanning a real person using multi-view stereo reconstruction. Our virtual humans are built upon a holistic character model and feature a detailed skeleton, fingers, eyes, teeth, and a rich set of facial blendshapes. Furthermore, due to the careful selection of techniques and technology, our reconstructed humans are quite realistic in terms of both geometry and texture. Since we represent our models as single-layer triangle meshes and animate them through standard skeleton-based skinning and facial blendshapes, our characters can be used in standard VR engines out of the box. By optimizing for computation time and minimizing manual intervention, our reconstruction pipeline is capable of processing whole characters in less than ten minutes.
Estimation of cobalt coating layer thickness on acicular Fe<sub>3</sub>O<sub>4</sub>powder using HR-TEM (2001)

The thicknesses of cobalt coating layers grown on four different types of acicular Fe 3 O 4 particles were estimated from the analysis of lattice images and from the determination of the Co depth profile using high-resolution transmission electron microscopy (HR-TEM) coupled with an energy dispersive X-ray (EDX) analysis system. It was observed that the coating thickness might vary with the crystallite size resulting from the growth mechanism of the coating layer. In this study, two different layer thicknesses, 40 Å and less than 10 Å, were evidenced for crystallite sizes of 390 Å and 350 Å, respectively.
Methanol steam‐reforming in a catalytic fixed bed reactor (1997)

Abstract Designing an appropriate methanol steam reformer requires detailed knowledge about the processes within such a reactor. Thus, the axial temperature and concentration gradients and catalyst ageing were investigated. It was found that for a fresh catalyst load, the catalyst located in the reactor entrance was most active during the experiment. The activity of this part of the catalyst bed decreased after some time of operation due to ageing. With further operation, the most active zone moved through the catalyst bed. From the results concerning hydrogen production and catalyst degradation, the necessary amount of catalyst for a mobile PEMFC‐system can be estimated.
Role of Bound Water on the Viscosity of Nanometric Alumina Suspensions (2005)

Rheological properties of aqueous nanometric alumina suspensions with fructose as dispersant were investigated. Differential scanning calorimetry and thermogravimetry indicated that a significant fraction of the water is immobilized by the nanometric particles in the suspension. This in turn increased the viscosity of the suspensions. It was also shown that the addition of monosaccharides, fructose in particular, reduced the viscosity of the suspensions dramatically. Nuclear magnetic resonance experiments indicated that a monolayer of fructose was adsorbed on the particle surfaces. The reduction in viscosity could be attributed to release of water imposed by fructose adsorption on the alumina particles present.
Protein kinase A-dependent binding of a nuclear factor to the 21-base-pair repeat of the human T-cell leukemia virus type I long terminal repeat (1990)

The long terminal repeat (LTR) of the human T-cell leukemia virus type I (HTLV-I) contains an imperfect repeat of 21 nucleotides which governs the response to the virus trans-activator protein tax and to cyclic AMP. In a murine thymocyte cell line defective in the catalytic subunit of protein kinase A, the response of the HTLV-I LTR to cyclic AMP is abolished and the response to tax is substantially diminished. This report shows that a factor present in nuclear extracts of wild-type cells binds to the HTLV-I 21-nucleotide sequence and that this binding activity is missing from the extracts of protein kinase A-defective cells. Treatment of nuclear extracts of protein kinase A-defective cells with the bovine protein kinase A catalytic subunit restores the binding activity, whereas treatment of wild-type nuclear extracts with a protein phosphatase destroys the binding activity. The binding factor is referred to as protein kinase A-dependent factor (PKAF). These results indicate that in murine thymocytes the response of the HTLV-I LTR to cyclic AMP depends upon the binding of a phosphorylated protein to the 21-nucleotide repeat sequence and that the response to tax is partially dependent upon binding of the phosphorylated protein. The results suggest a model in which the phosphorylation of a transcription factor by protein kinase A regulates HTLV-I gene expression.
Development of a Variable Emittance Radiator Based on a Perovskite Manganese Oxide (2003)

Two types of thermal control materials developed for space use are described. These new materials use a variable emittance radiator called the smart radiation device (SRD). The SRDs are based on La 0.82 Sr 0.175 MnO 3 and La 0.7 Ca 0.3 MnO 3 and comprise a thin and light ceramic tile. The materials undergo a metal-insulator transition near room temperature, and this allows the infrared emissivity of the device to change from low to high as the temperature is increased from 173 to 375 K. This is beneficial for thermal control applications on spacecraft. For example, bonded only to the external surface of the spacecraft, the SRD controls the heat radiated to deep space without electrical instruments or mechanical parts. Its function is similar to the thermal louver, but the SRD is lighter. This new device reduces the energy consumption of the electrical heater for thermal control and decreases the weight and the cost of the thermal control system. Optical properties, such as the total hemispherical emittance and the solar absorptance, have been measured. Space environmental simulation tests were performed with independent irradiation by protons, electrons, and UV on the ground. Degradation of the materials' optical properties is discussed.
Targeting Protein-Protein Interactions with Trimeric Ligands: High Affinity Inhibitors of the MAGUK Protein Family (2015)

PDZ domains in general, and those of PSD-95 in particular, are emerging as promising drug targets for diseases such as ischemic stroke. We have previously shown that dimeric ligands that simultaneously target PDZ1 and PDZ2 of PSD-95 are highly potent inhibitors of PSD-95. However, PSD-95 and the related MAGUK proteins contain three consecutive PDZ domains, hence we envisioned that targeting all three PDZ domains simultaneously would lead to more potent and potentially more specific interactions with the MAGUK proteins. Here we describe the design, synthesis and characterization of a series of trimeric ligands targeting all three PDZ domains of PSD-95 and the related MAGUK proteins, PSD-93, SAP-97 and SAP-102. Using our dimeric ligands targeting the PDZ1-2 tandem as starting point, we designed novel trimeric ligands by introducing a PDZ3-binding peptide moiety via a cysteine-derivatized NPEG linker. The trimeric ligands generally displayed increased affinities compared to the dimeric ligands in fluorescence polarization binding experiments and optimized trimeric ligands showed low nanomolar inhibition towards the four MAGUK proteins, thus being the most potent inhibitors described. Kinetic experiments using stopped-flow spectrometry showed that the increase in affinity is caused by a decrease in the dissociation rate of the trimeric ligand as compared to the dimeric ligands, likely reflecting the lower probability of simultaneous dissociation of all three PDZ ligands. Thus, we have provided novel inhibitors of the MAGUK proteins with exceptionally high affinity, which can be used to further elucidate the therapeutic potential of these proteins.