gebhart/patrepeval huggingface dataset:
Studies of the plasma membrane H+-ATPase of yeast and plants (1992)

Conference Article| August 01 1992 Studies of the plasma membrane H+-ATPase of yeast and plants Ramon Serrano; Ramon Serrano * 1European Molecular Biology Laboratory, Meyerhoffstrasse 1, 6900 Heidelberg, Germany Search for other works by this author on: This Site PubMed Google Scholar Jose M. Villalba; Jose M. Villalba 1European Molecular Biology Laboratory, Meyerhoffstrasse 1, 6900 Heidelberg, Germany Search for other works by this author on: This Site PubMed Google Scholar Michael G. Palmgren; Michael G. Palmgren 1European Molecular Biology Laboratory, Meyerhoffstrasse 1, 6900 Heidelberg, Germany Search for other works by this author on: This Site PubMed Google Scholar Francisco Portillo; Francisco Portillo 1European Molecular Biology Laboratory, Meyerhoffstrasse 1, 6900 Heidelberg, Germany Search for other works by this author on: This Site PubMed Google Scholar Antonio Parets-Soler; Antonio Parets-Soler 1European Molecular Biology Laboratory, Meyerhoffstrasse 1, 6900 Heidelberg, Germany Search for other works by this author on: This Site PubMed Google Scholar Marta Roldan; Marta Roldan 1European Molecular Biology Laboratory, Meyerhoffstrasse 1, 6900 Heidelberg, Germany Search for other works by this author on: This Site PubMed Google Scholar Charles Ferguson; Charles Ferguson 1European Molecular Biology Laboratory, Meyerhoffstrasse 1, 6900 Heidelberg, Germany Search for other works by this author on: This Site PubMed Google Scholar Consuelo Montesinos Consuelo Montesinos 1European Molecular Biology Laboratory, Meyerhoffstrasse 1, 6900 Heidelberg, Germany Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1992) 20 (3): 562–566. https://doi.org/10.1042/bst0200562 Article history Received: April 24 1992 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn MailTo Cite Icon Cite Get Permissions Citation Ramon Serrano, Jose M. Villalba, Michael G. Palmgren, Francisco Portillo, Antonio Parets-Soler, Marta Roldan, Charles Ferguson, Consuelo Montesinos; Studies of the plasma membrane H+-ATPase of yeast and plants. Biochem Soc Trans 1 August 1992; 20 (3): 562–566. doi: https://doi.org/10.1042/bst0200562 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search This content is only available as a PDF. © 1992 Biochemical Society1992 Article PDF first page preview Close Modal You do not currently have access to this content.
Hyperspectral imaging for presumptive identification of bacterial colonies on solid chromogenic culture media (2016)

BioMérieux is automating the microbiology laboratory in order to reduce cost (less manpower and consumables), to improve performance (increased sensitivity, machine algorithms) and to gain traceability through optimization of the clinical laboratory workflow. In this study, we evaluate the potential of Hyperspectral imaging (HSI) as a substitute to human visual observation when performing the task of microbiological culture interpretation. Microbial colonies from 19 strains subcategorized in 6 chromogenic classes were analyzed after a 24h-growth on a chromogenic culture medium (chromID® CPS Elite, bioMérieux, France). The HSI analysis was performed in the VNIR region (400-900 nm) using a linescan configuration. Using algorithms relying on Linear Spectral Unmixing, and using exclusively Diffuse Reflectance Spectra (DRS) as input data, we report interclass classification accuracies of 100% using a fully automatable approach and no use of morphological information. In order to eventually simplify the instrument, the performance of degraded DRS was also evaluated using only the most discriminant 14 spectral channels (a model for a multispectral approach) or 3 channels (model of a RGB image). The overall classification performance remains unchanged for our multispectral model but is degraded for the predicted RGB model, hints that a multispectral solution might bring the answer for an improved colony recognition.
A viral aphrodisiac in the cricket <i>Gryllus texensis</i> (2014)

We identified the insect iridovirus IIV-6/CrIV as a pathogen of the cricket Gryllus texensis using electron microscopy (EM) and polymerase chain reaction (PCR) analysis. EM showed that the virus attacks the fat body, an organ important for protein production, immune function and lipid storage. During infection the fat body hypertrophied, but egg production withered, leaving the lateral oviducts empty of eggs; the females were effectively sterile. EM of the testis of infected males suggests that the testis was not invaded by the virus, although sperm taken from the spermatophores of infected males showed little or no motility. Nevertheless, males and females continued to mate when infected. In fact, infected males were quicker to court females than uninfected controls. The virus benefits from the continued sexual behaviour of its host; transmission studies show that the virus can be spread through sexual contact. Sickness behaviour, the adaptive reduction of feeding and sexual behaviour that is induced by an activated immune system, was absent in infected crickets. Total haemolymph protein was reduced, as was phenoloxidase activity, suggesting a reduction in immune protein production by the fat body. The evidence suggests that during IIV-6/CrIV infection, the immune signal(s) that induces sickness behaviour is absent. Curtailment of a host's sickness behaviour may be necessary for any pathogen that is spread by host sexual behaviour.
The geomicrobiology of CO2 geosequestration: a focused review on prokaryotic community responses to field-scale CO2 injection (2015)

Our primary research paper (Mu et al., 2014) demonstrated selective changes to a deep subsurface prokaryotic community as a result of CO2 stress. Analyzing geochemical and microbial 16S rRNA gene profiles, we evaluated how in situ prokaryotic communities responded to increased CO2 and the presence of trace organic compounds, and related temporal shifts in phylogeny to changes in metabolic potential. In this focused review, we extend upon our previous discussion to present analysis of taxonomic unit co-occurrence profiles from the same field experiment, to attempt to describe dynamic community behavior within the deep subsurface. Understanding the physiology of the subsurface microbial biosphere, including how key functional groups integrate into the community, will be critical to determining the fate of injected CO2. For example, community-wide network analyses may provide insights to whether microbes cooperatively produce biofilm biomass, and/or biomineralize the CO2, and hence, induce changes to formation porosity or changes in electron flow. Furthermore, we discuss potential impacts to the feasibility of subsurface CO2 storage of selectively enriching for particular metabolic functions (e.g., methanogenesis) as a result of CO2 injection.
Glucose and nonmaintained pH decrease expression of the accessory gene regulator (agr) in Staphylococcus aureus (1992)

The effect of glucose on accessory gene regulator (agr) expression in Staphylococcus aureus was examined. agr is a global regulator that affects the expression of numerous genes, including those for some factors implicated in virulence, such as toxic shock syndrome toxin 1, alpha-hemolysin, and protein A. The agr locus determines two divergent transcripts, designated RNAII and RNAIII. RNAII contains four open reading frames (agrABCD), and RNAIII encodes delta-hemolysin. The mechanisms responsible for agr-mediated regulation are not well understood, but it appears that the RNAIII transcript plays a central role in the regulation of a number of target genes, including those for alpha-hemolysin (hla), beta-hemolysin (hlb), protein A (spa), and staphylococcal enterotoxin B (seb+). In this study, S. aureus cultures were grown either in a shake flask system with a complex medium or in a fermentor system with a completely defined medium in which the pH and glucose concentration were maintained. Northern (RNA) blot analysis revealed that a dramatic reduction in agr expression was apparent only when the cultures contained glucose and when the pH was 5.5 or was not maintained. The effect of glucose on two agr target genes, sec+ and hla, was also studied. Glucose-containing cultures produced less sec+ and hla mRNAs at maintained pH (6.5). In addition, the glucose effect on sec+ and hla was enhanced under conditions that inhibited agr expression (i.e., pH 5.5 or a nonmaintained pH).
Improving Object Detection With One Line of Code. (2017)

Non-maximum suppression is an integral part of the object detection pipeline.
First, it sorts all detection boxes on the basis of their scores. The detection
box M with the maximum score is selected and all other detection boxes with a
significant overlap (using a pre-defined threshold) with M are suppressed. This
process is recursively applied on the remaining boxes. As per the design of the
algorithm, if an object lies within the predefined overlap threshold, it leads
to a miss. To this end, we propose Soft-NMS, an algorithm which decays the
detection scores of all other objects as a continuous function of their overlap
with M. Hence, no object is eliminated in this process. Soft-NMS obtains
consistent improvements for the coco-style mAP metric on standard datasets like
PASCAL VOC 2007 (1.7\% for both R-FCN and Faster-RCNN) and MS-COCO (1.3\% for
R-FCN and 1.1\% for Faster-RCNN) by just changing the NMS algorithm without any
additional hyper-parameters. Further, the computational complexity of Soft-NMS
is the same as traditional NMS and hence it can be efficiently implemented.
Since Soft-NMS does not require any extra training and is simple to implement,
it can be easily integrated into any object detection pipeline. Code for
Soft-NMS is publicly available on GitHub \url{http://bit.ly/2nJLNMu}.
Face recognition in the wild. (2013)

Research in face recognition deals with problems related to Age, Pose, Illumination and Expression (A-PIE), and seeks approaches that are invariant to these factors. Video images add a temporal aspect to the image acquisition process. Another degree of complexity, above and beyond A-PIE recognition, occurs when multiple pieces of information are known about people, which may be distorted, partially occluded, or disguised, and when the imaging conditions are totally unorthodox! A-PIE recognition in these circumstances becomes really “wild” and therefore, Face Recognition in the Wild has emerged as a field of research in the past few years. Its main purpose is to challenge constrained approaches of automatic face recognition, emulating some of the virtues of the Human Visual System (HVS) which is very tolerant to age, occlusion and distortions in the imaging process. HVS also integrates information about individuals and adds contexts together to recognize people within an activity or behavior. Machine vision has a very long road to emulate HVS, but face recognition in the wild, using the computer, is a road to perform face recognition in that path. In this thesis, Face Recognition in the Wild is defined as unconstrained face recognition under A-PIE+; the (+) connotes any alterations to the design scenario of the face recognition system. This thesis evaluates the Biometric Optical Surveillance System (BOSS) developed at the CVIP Lab, using low resolution imaging sensors. Specifically, the thesis tests the BOSS using cell phone cameras, and examines the potential of facial biometrics on smart portable devices like iPhone, iPads, and Tablets. For quantitative evaluation, the thesis focused on a specific testing scenario of BOSS software using iPhone 4 cell phones and a laptop. Testing was carried out indoor, at the CVIP Lab, using 21 subjects at distances of 5, 10 and 15 feet, with three poses, two expressions and two illumination levels. The three steps (detection, representation and matching) of the BOSS system were tested in this imaging scenario. False positives in facial detection increased with distances and with pose angles above ± 15°. The overall identification rate (face detection at confidence levels above 80%) also degraded with distances, pose, and expressions. The indoor lighting added challenges also, by inducing shadows which affected the image quality and the overall performance of the system. While this limited number of subjects and somewhat constrained imaging environment does not fully support a “wild” imaging scenario, it did provide a
Structure-based virtual screening of novel, high-affinity BRD4 inhibitors (2014)

Bromodomains (BRDs) are a diverse family of evolutionarily conserved protein-interaction modules. Among various members of the bromodomain and extra terminal domain family, BRD4 is found to be an important target for many diseases such as cancer, acute myeloid leukemia, multiple myeloma, Burkitt's lymphoma, etc. Therefore, in this study an attempt has been made to screen compounds from NCI Diversity, Drug Bank and Toslab Databases targeting the Kac binding site of BRD4 using molecular docking, molecular dynamics simulations, MM-PB/GBSA binding free energy calculations and steered molecular dynamics simulations. Using virtual screening and docking, we have identified 11 inhibitors. These new inhibitors exhibit binding energy values higher than that of the (+)JQ1 inhibitor which is effective against BRD4. However, due to the toxicity of (+)JQ1, the designing of new inhibitors becomes significantly important. Thus, these new 11 ligands were systematically analyzed using other computational investigations. Results reveal that the compounds ZINC01411240, ZINC19632618 and ZINC04818522 could be potential drug candidates for targeting BRD4. It can also be seen from the results that there is a linear relationship between the results obtained from the SMD simulation and free energy obtained from the MM-PBSA/GBSA approach. This study clearly illustrates that the steered molecular dynamics can be effectively used for the design of new inhibitors.
KarMMa-4: Idecabtagene vicleucel (ide-cel, bb2121), a BCMA-directed CAR T-cell therapy in high-risk newly diagnosed multiple myeloma. (2021)

TPS8053 Background: High-risk (R-ISS stage III) newly diagnosed multiple myeloma (NDMM) has a poor prognosis (median PFS, 29 mo), highlighting the need for novel disease-targeting approaches (Palumbo A, et al. J Clin Oncol 2015;33:2863-2869). Ide-cel, a BCMA-directed CAR T cell therapy, demonstrated deep, durable responses in heavily pretreated patients (pts) with relapsed and refractory MM (RRMM; Raje N, et al. N Engl J Med 2019;380:1726-1737; Munshi NC, et al. J Clin Oncol 2020;38[suppl 15]. Abstract 8503), including those with high-risk (R-ISS stage III) RRMM. In this population, earlier use of ide cel—when there may be more bone marrow reserve, more healthy peripheral blood mononuclear cells, a less compromised immune status, and less extensive disease to debulk before cell therapy—may result in improved outcomes vs standard therapies and offer an opportunity to replace transplant with CAR T cell therapy. Methods: KarMMa-4 (NCT04196491), a multicenter, open-label, phase 1, single-arm study, is currently evaluating ide-cel in pts with high-risk NDMM, defined as R-ISS stage III (ISS stage III [serum ß 2 microglobulin ≥ 5.5 mg/L] and cytogenetic abnormalities t(4;14), del(17p), and/or t(14;16) by interphase FISH; or ISS stage III and serum LDH > ULN). Pts must have received ≤ 3 cycles of the induction regimens listed below, be aged ≥ 18 years, and have ECOG PS 0-1. Nonsecretory MM and CNS involvement are exclusion criteria. Pts can enroll between induction cycles 1 and 3. Permitted cycle 1 regimens are carfilzomib + lenalidomide (LEN) + dexamethasone (DEX) ± daratumumab (DARA; KRd ± DARA), LEN + bortezomib (BOR) + DEX ± DARA (RVd ± DARA), or cyclophosphamide + BOR + DEX (CyBorD). Induction cycles 2-4 are limited to KRd or RVd, with DEX omitted during cycle 3. Pts will undergo T cell collection via leukapheresis after cycle 3, and ide-cel will be manufactured during cycle 4. Stem cell collection for future use may be conducted after cycle 3 (following leukapheresis) or 4 (before lymphodepletion). Ide-cel is infused after 2 days of rest following lymphodepletion with 3 days of fludarabine 30 mg/m 2 + cyclophosphamide 300 mg/m 2 . LEN-based maintenance may be provided upon bone marrow recovery or 90 days after ide-cel infusion, whichever is later. Dose-limiting toxicity and safety are the primary endpoints. Secondary endpoints include complete response (CR) rate and overall response rate, duration of response, time to CR, time to start of maintenance, feasibility of initiating maintenance, PFS, overall survival, and pharmacokinetics. Exploratory endpoints include LEN maintenance safety, minimal residual disease, immunogenicity and biomarkers. The starting ide-cel target dose is 450 × 10 6 CAR+ T cells, with dose escalation/de-escalation (150, 300, and 800 × 10 6 CAR+ T cells). Upon determination of optimal target dose, 12 pts will be enrolled in the dose-expansion phase. Clinical trial information: NCT04196491.
Electrically stimulated cell membrane breakdown in human placenta TL and lung cancer cell A549 in 3D trap arrays on Si substrate (2004)

We have integrated the human placenta TL and lung cancer A549 cells on Si substrate using 3D trenched trap arrays and investigated the cell membranes breakdown behavior under electric field for bio-medical application.
Research on the charge mode of series-connected batteries (2008)

Traditional charge mode based on terminal voltage of batteries results in serious overcharge since status of battery cell is not known, it seriously shortens batteriespsila cycle life, even results in hidden trouble of safety. A new type of charge mode combining BMS (Battery Management Systems) and charger is presented in this paper. By data sharing between BMS and charger, the charger can known very well information about each batterypsilas voltage, temperature and failures, according to this, charger can adjust charging current. Therefore, it ensures no overcharge of any battery occurs and reduces influences that charging have on batteries, and increases safety of charging process. In this paper, principles of model parameter identification under the charge mode and an even more scientific consistency evaluation based on capacity and internal resistance are also elaborated.
Neutron-driven gamma-ray laser (2023)

A lasing cylinder emits laser radiation at a gamma-ray wavelength of 0.87 .ANG. when subjected to an intense neutron flux of about 400 eV neutrons. A 250 .ANG. thick layer of Be is provided between two layers of 100 .ANG. thick layer of .sup.57 Co and these layers are supported on a foil substrate. The coated foil is coiled to form the lasing cylinder. Under the neutron flux .sup.57 Co becomes .sup.58 Co by neutron absorption. The .sup.58 Co then decays to .sup.57 Fe by 1.6 MeV proton emission. .sup.57 Fe then transitions by mesne decay to a population inversion for lasing action at 14.4 keV. Recoil from the proton emission separates the .sup.57 Fe from the .sup.57 Co and into the Be, where Mossbauer emission occurs at a gamma-ray wavelength.
Glycation as the glucose link to diabetic complications. (2000)

Hyperglycemia is considered a key causal factor in the development of diabetic vascular complications and can mediate its adverse effects through multiple pathways. This was confirmed for microangiopathy in the Diabetes Control and Complications Trial study for type 1 diabetes and corroborated for type 2 diabetes by the United Kingdom Prospective Diabetes Study published in 1998. Prevention of diabetic complications requires at least control of glycemia. This article briefly summarizes the evidence that strongly supports the role of hyperglycemia in vascular complications. After outlining the role of the polyol pathway, protein kinase C, and oxidative stress, the author focuses on one of the key biochemical mechanisms for this pathologic process: the direct deleterious action of glucose and other sugars on proteins, known as glycation or nonenzymatic glycosylation. Results of animal studies and phase III clinical trials reveal that the inhibition of this process attenuates the development of a range of these complications.
SMPTE Tutorial: Gray-Scale Transformations of Digital Film Data for Display, Conversion, and Film Recording (1993)

The following paper has been submitted to the SMPTE Working Group on Digital Pictures This group is charged with the task of developing a recommended practice to enable conversion of film.video, nnd computer-generated inuiges. Since the work is not presently complete, this paper represents work in progress. It is beiirg odfered for its tutorial vulucin presenting consideration and relationships involved with multiniediu digital image e.vchangc.
Burn Resuscitation with Two Doses of 4 mL/kg Hypertonic Saline Dextran Provides Sustained Fluid Sparing: A 48-Hour Prospective Study in Conscious Sheep (2000)

An initial 4 mL x kg(-1) dose of HSD reduces fluid requirements early after burn, and a second dose administered after an appropriate interval may prolong volume sparing through 48 hours. An 8 mL x kg(-1) continuously infused initial dose was without prolonged fluid sparing effect. The volume-sparing effect of HSD is thus dependent on all of the following: dose, dosing interval, and infusion rate.
The activity of topoisomerases is related to the grade and stage in human renal cell carcinoma. (1995)

Human renal cell carcinomas are quite resistant to chemotherapy. There are no good anticancer agents effective for this disease. Recently, many anticancer agents were found to act as topoisomerase inhibitors and this is closely correlated with their anticancer activity. To assess the efficacy of anticancer agents as topoisomerase inhibitors on renal cell carcinomas, we examined the activity of topoisomerase I and topoisomerase II in 60 primary human renal cell carcinomas using DNA relaxation or DNA decatenation. Significant topoisomerase I and topoisomerase II activities were observed in 39(65%) or 22(36.7%) cases respectively. Topoisomerase II positive cases were more frequent in higher grade cancers (p < 0.01). The topoisomerase I positive rate did not differ in all grades. Our results showed that human renal cell carcinomas had significant topoisomerase I or topoisomerase II activities that were targets for some topoisomerase inhibitors. Therefore these agents could be good candidates for the chemotherapy of human renal cell carcinomas.
The effect of ultrasonic irradiation on the skin penetration of a potent humectant, sodium hyaluronate. (1997)

The effects of ultrasonic irradiation on the skin-moisturing and the skin penetration of sodium hyaluronate (HA) were measured in hairless rats. Ultrasound (2.15 MHz) with different power and pulse duty was applied for 10 min on the abdominal site which was pretreated with 1% HA for 3 h. Relative conductance on the skin surface was increased by the ultrasonication. The content of HA in the stratum corneum (measured using fluorescence-labeled HA) was also increased by the treatment. In addition, a good relationship was observed between the two values. The ultrasonic treatment may be utilized as a new tool to increase the HA penetration and the humectant efficacy into and on the skin surface.
Performance Indicators What You Need to Know... (2010)

Business-led, results-orientated maintenance management is driven by KPIs (Key Performance Indicators), also known as measures or metrics. There are two types of indicators - lagging and leading. Success as an engineering manager depends on understanding the basic nature and use of each type - together with a resolute bias for action.