gebhart/patrepeval huggingface dataset:
Magnetic Cell Separation for Research and Clinical Applications (2006)

This chapter contains sections titled: Introduction MACS® Technology The Concept Magnetic Separation Strategies Magnetic Labeling Strategies and Reagents Superparamagnetic MicroBeads Column Technology and Research Separators CliniMACS®Plus Instrument, and Accessories Magnetic Cell Sorting for Clinical Applications Stem Cell Enrichment for Graft Engineering in Hematological Disorders NK Cells: CD56 and CD3 T-Cell Subset Graft Engineering Strategies Antigen-Specific T Cells: Cytokine Capture System Dendritic Cells (DC): CD14-derived DC, BDCA-1, BDCA-4 Into the Future: Cardiac Regeneration Using CD133+ Stem Cells
Generation of Radially Polarised Beams from Optical Fibers (2008)

We generate radially-polarised beams, for the first time with a novel, phase-engineered fiber that possesses these spatial patterns as stable eigenmodes. With conversion-efficiencies >99.7%, this is an attractive alternative to complex free-space techniques used currently.
Deaggregation of Nanodiamond Powders Using Salt- and Sugar-Assisted Milling (2010)

Diamond particles of 5-10 nm in size can be produced in large quantities by denonating oxygen-lean explosives in a closed chamber. They have numerous useful properties and are used in applications ranging from lubricants to drug delivery. Aggregation of diamond nanoparticles is limiting wider use of this important carbon nanomaterial because most applications require single separated particles. We demonstrate that dry media assisted attrition milling is a simple, inexpensive, and efficient alternative to the current ways of deaggregating of nanodiamond. This technique uses water-soluble nontoxic and noncontaminating crystalline compounds, such as sodium chloride or sucrose. When milling is complete, the media can be easily removed from the product by water rinsing, which provides an advantage when compared to milling with ceramic microbeads. Using the dry media assisted milling with subsequent pH adjustment, it is possible to produce stable aqueous nanodiamond colloidal solutions with particles <10 nm in diameter, which corresponds to 1-2 primary nanodiamond particles. The study of milling kinetics and the characterization of the produced nanodiamond colloids led us to conclude that aggregates of less than 200 nm in diameter, observed at the tail of the pore size distribution of milled nanodiamond, are loosely bonded and rather dynamic in nature. Color change observed in ND colloids upon shifting their pH toward the basic end allowed us to demonstrate that the coloration comes from the light interaction with colloidal particles and not from an increase in nondiamond carbon content.
Ganglioside GD2 identifies breast cancer stem cells and promotes tumorigenesis (2012)

Cancer stem cells (CSCs) are a small subpopulation of cancer cells that have increased resistance to conventional therapies and are capable of establishing metastasis. However, only a few biomarkers of CSCs have been identified. Here, we report that ganglioside GD2 (a glycosphingolipid) identifies a small fraction of cells in human breast cancer cell lines and patient samples that are capable of forming mammospheres and initiating tumors with as few as 10 GD2+ cells. In addition, the majority of GD2+ cells are also CD44hiCD24lo, the previously established CSC-associated cell surface phenotype. Gene expression analysis revealed that GD3 synthase (GD3S) is highly expressed in GD2+ as well as in CD44hiCD24lo cells and that interference with GD3S expression, either by shRNA or using a pharmacological inhibitor, reduced the CSC population and CSC-associated properties. GD3S knockdown completely abrogated tumor formation in vivo. Also, induction of epithelial-mesenchymal transition (EMT) in transformed human mammary epithelial cells (HMLER cells) dramatically increased GD2 as well as GD3S expression in these cells, suggesting a role of EMT in the origin of GD2+ breast CSCs. In summary, we identified GD2 as a new CSC-specific cell surface marker and GD3S as a potential therapeutic target for CSCs, with the possibility of improving survival and cure rates in patients with breast cancer.
The design of a vibration transducer to monitor the integrity of dental implants (1998)

Bone-anchored titanium implants are being used increasingly to provide support for prostheses replacing missing teeth in edentulous and partially dentate patients. A technique is required to monitor bone formation at the implant-tissue interface during healing, and also to check whether there has been bone loss from around the top of the implant. One possible method is to screw a beam into the implanted fixture and to measure the first flexural resonance frequency of the resulting system. This resonance frequency is affected by both the exposed length of fixture and the stiffness of the interface between the implant and the bone. This paper describes the design of a beam-like transducer for clinical trials of the technique. The sensitivity of the transducer resonance frequency to the changes of interest is dependent on the thickness and length of the beam element. However, the choice of these dimensions is constrained by the need to avoid closely spaced resonances. The performance of different transducer shapes and the influence of the thickness and length of the beam element in the transducer has been studied. The results have been used to finalize a transducer design for the clinical trials.
Frequency-Hopped Single-Sideband Modulation for Mobile Radio (1982)

A frequency-hopped single-sideband (SSB) modulation with pilot tone (FH-SSB-PT) transmission scheme is described for mobile radio. The single-sideband signal changes its carrier frequency every τ seconds, and the sequence of carrier frequencies is controlled by a suitable scrambling code. Co-channel cells using the same frequency band in the mobile radio system use different scrambling codes with the result that co-channel interference is altered from cross-talk to random noise if τ is small. In the presence of Rayleigh fading, frequency hopping improves reception for stationary vehicles, and does not degrade the performance for moving vehicles. Further, the technique of frequency hopping may offer communications privacy. Expressions for s/n are derived for SSB, SSB plus pilot tone, and FH-SSB-PT in the presence of Rayleigh fading. Co-channel interference effects are also considered.
Measurement of Access Flow During Hemodialysis Using the Constant Infusion Approach (1998)

With reversed placement of blood lines and with a peripheral arteriovenous access, hemodialysis recirculation (R) consists of a local access component, and a central cardiopulmonary component that must be separated for the calculation of access flow (Qac) using indicator dilution principles. With indicator injections that follow constant infusion principles Qac = (1 - Rx)/(Rx(1 - CPR)) x (Qb,x - UFR), where Qb is the extracorporeal blood flow, where UFR is the ultrafiltration rate, and where the index x indicates reversed placement of blood lines. CPR, the amount of cardiopulmonary recirculation (CPR = Qac/CO) is determined from two recirculation measurements with correct (index n) and with reversed (index x) placement of blood lines CPR = Rn(1 - Rx)/Rx(1 - Rn) x (Qb,x - UFR)/Qb,n. Qac was measured in 11 hemodialysis (HD) patients using a thermodilution device tested in an in vitro set-up based on constant infusion principles. Mean Qac was 1.135 L/min and 1.054 L/min for measurements done early and late in dialysis. The coefficient of variation was +/-7.3% and +/-8.6%, respectively. Repeated measurements of access flow in HD patients showed good reproducibility (Qac.1 = 1.01*Qac.0, r2 = 0.98), with the regression line not different from the line of identity; however, in vivo results remain to be validated by an independent technique.
A review of progress on nano-aperture VCSEL (2008)

This paper reviews the progress on nano-aperture vertical-cavity surface-emitting lasers (VCSELs). The design, fabrication, and polarization control of nano-aperture VCSELs are reviewed. With the nanoaperture evolving from conventional circular and square aperture to unique C-shaped, H-shaped, I-shaped, and bowtie-shaped aperture, both the near-field intensity and near-field beam confinement from nanoaperture VCSELs are significantly improved. As a high-intensity compact light source with sub-100nm spot size, nano-aperture VCSELs are promising to realize many new near-field optical systems and applications.
Acetylcholine does not play a major role in mediating the endocrine responses to ghrelin, a natural ligand of the GH secretagogue receptor, in humans (2003)

The endocrine activities of ghrelin are not affected significantly by cholinergic enhancement and muscarinic blockade. Thus, acetylcholine does not play a major role in the endocrine actions of ghrelin. Moreover, as the cholinergic system influences GH secretion via modulation of somatostatin release, the present data agree with the assumption that ghrelin is partially refractory to the influence of somatostatin.
MicroRNA Expression Profiling in Human Ovarian Cancer: <i>miR-214</i> Induces Cell Survival and Cisplatin Resistance by Targeting <i>PTEN</i> (2008)

MicroRNAs (miRNA) represent a novel class of genes that function as negative regulators of gene expression. Recently, miRNAs have been implicated in several cancers. However, aberrant miRNA expression and its clinicopathologic significance in human ovarian cancer have not been well documented. Here, we show that several miRNAs are altered in human ovarian cancer, with the most significantly deregulated miRNAs being miR-214, miR-199a*, miR-200a, miR-100, miR-125b, and let-7 cluster. Further, we show the frequent deregulation of miR-214, miR-199a*, miR-200a, and miR-100 in ovarian cancers. Significantly, miR-214 induces cell survival and cisplatin resistance through targeting the 3'-untranslated region (UTR) of the PTEN, which leads to down-regulation of PTEN protein and activation of Akt pathway. Inhibition of Akt using Akt inhibitor, API-2/triciribine, or introduction of PTEN cDNA lacking 3'-UTR largely abrogates miR-214-induced cell survival. These findings indicate that deregulation of miRNAs is a recurrent event in human ovarian cancer and that miR-214 induces cell survival and cisplatin resistance primarily through targeting the PTEN/Akt pathway.
Synthesis of Methanol in a Methanol-Rich Liquid Phase (2024)

A novel method for synthesizing methanol from CO and H<sub>2</sub> in a slurry reactor is described and experimental results are presented. At the temperatures and pressures used (100-160°C, 50 atm) the methanol product is liquid and constitutes the bulk of the liquid phase. Small amounts of methyl formate (4 to 5%) and other products (less than 1% total) are formed. A heterogeneous catalyst (copper chromite) and a homogeneous catalyst (an alkali salt) are used in a single reactor. It appears likely that the reaction proceeds in two steps--carbonylation of methanol to methyl formate and then hydrogenation of the formate to two moles of methanol. Experiments were carried out in a 3 00 ml autoclave with continuous gas feed and intermittent liquid withdrawal. In situ reduction of the copper chromite was found to be as effective as external reduction and was used in all runs. A barium-promoted copper chromite showed substantially less deactivation than a manganese promoted one. Of six homogeneous catalysts tested the most effective was potassium methoxide, although some reaction occurred with all the homogeneous catalysts used. The rate of reaction was found to vary with gas feed rate--an unexpected result. It is proposed that this is due to the presence of small amounts of an inhibiting agent which accumulates in the liquid at low flow rates.
Robust tumor morphometry in multispectral fluorescence microscopy (2009)

Morphological and architectural characteristics of primary tissue compartments, such as epithelial nuclei (EN) and cytoplasm, provide important cues for cancer diagnosis, prognosis, and therapeutic response prediction. We propose two feature sets for the robust quantification of these characteristics in multiplex immunofluorescence (IF) microscopy images of prostate biopsy specimens. To enable feature extraction, EN and cytoplasm regions were first segmented from the IF images. Then, feature sets consisting of the characteristics of the minimum spanning tree (MST) connecting the EN and the fractal dimension (FD) of gland boundaries were obtained from the segmented compartments. We demonstrated the utility of the proposed features in prostate cancer recurrence prediction on a multi-institution cohort of 1027 patients. Univariate analysis revealed that both FD and one of the MST features were highly effective for predicting cancer recurrence (p ≤ 0.0001). In multivariate analysis, an MST feature was selected for a model incorporating clinical and image features. The model achieved a concordance index (CI) of 0.73 on the validation set, which was significantly higher than the CI of 0.69 for the standard multivariate model based solely on clinical features currently used in clinical practice (p < 0.0001). The contributions of this work are twofold. First, it is the first demonstration of the utility of the proposed features in morphometric analysis of IF images. Second, this is the largest scale study of the efficacy and robustness of the proposed features in prostate cancer prognosis.
Receptor-Binding Domain of Severe Acute Respiratory Syndrome Coronavirus Spike Protein Contains Multiple Conformation-Dependent Epitopes that Induce Highly Potent Neutralizing Antibodies (2005)

The spike (S) protein of severe acute respiratory syndrome associated coronavirus (SARS-CoV) is a major antigenic determinant capable of inducing protective immunity. Recently, a small fragment on the SARS-CoV S protein (residues 318-510) was characterized as a minimal receptor-binding domain (RBD), which mediates virus binding to angiotensin-converting enzyme 2, the functional receptor on susceptible cells. In this study, we demonstrated that a fusion protein containing RBD linked to human IgG1 Fc fragment (designated RBD-Fc) induced high titer of RBD-specific Abs in the immunized mice. The mouse antisera effectively neutralized infection by both SARS-CoV and SARS pseudovirus with mean 50% neutralization titers of 1/15,360 and 1/24,737, respectively. The neutralization determinants on the RBD of S protein were characterized by a panel of 27 mAbs isolated from the immunized mice. Six groups of conformation-dependent epitopes, designated as Conf I-VI, and two adjacent linear epitopes were identified by ELISA and binding competition assays. The Conf IV and Conf V mAbs significantly blocked RBD-Fc binding to angiotensin-converting enzyme 2, suggesting that their epitopes overlap with the receptor-binding sites in the S protein. Most of the mAbs (23 of 25) that recognized the conformational epitopes possessed potent neutralizing activities against SARS pseudovirus with 50% neutralizing dose ranging from 0.005 to 6.569 microg/ml. Therefore, the RBD of SARS S protein contains multiple conformational epitopes capable of inducing potent neutralizing Ab responses, and is an important target site for developing vaccines and immunotherapeutics.
Synthesis and Characterization of Lipooligosaccharide-Based Conjugates as Vaccine Candidates for <i>Moraxella</i> ( <i>Branhamella</i> ) <i>catarrhalis</i> (1998)

Moraxella (Branhamella) catarrhalis is an important cause of otitis media and sinusitis in children and of lower respiratory tract infections in adults. Lipooligosaccharide (LOS) is a major surface antigen of the bacterium and elicits bactericidal antibodies. Treatment of the LOS from strain ATCC 25238 with anhydrous hydrazine reduced its toxicity 20,000-fold, as assayed in the Limulus amebocyte lysate (LAL) test. The detoxified LOS (dLOS) was coupled to tetanus toxoid (TT) or high-molecular-weight proteins (HMP) from nontypeable Haemophilus influenzae through a linker of adipic acid dihydrazide to form dLOS-TT or dLOS-HMP. The molar ratios of dLOS to TT and HMP conjugates were 19:1 and 31:1, respectively. The antigenicity of the two conjugates was similar to that of the LOS, as determined by double immunodiffusion. Subcutaneous or intramuscular injection of both conjugates elicited a 50- to 100-fold rise in the geometric mean of immunoglobulin G (IgG) to the homologous LOS in mice after three injections and a 350- to 700-fold rise of anti-LOS IgG in rabbits after two injections. The immunogenicity of the conjugate was enhanced by formulation with monophosphoryl lipid A plus trehalose dimycolate. In rabbits, conjugate-induced antisera had complement-mediated bactericidal activity against the homologous strain and heterologous strains of M. catarrhalis. These results indicate that a detoxified LOS-protein conjugate is a candidate for immunization against M. catarrhalis diseases.
ClothFlow: A Flow-Based Model for Clothed Person Generation (2019)

We present ClothFlow, an appearance-flow-based generative model to synthesize clothed person for posed-guided person image generation and virtual try-on. By estimating a dense flow between source and target clothing regions, ClothFlow effectively models the geometric changes and naturally transfers the appearance to synthesize novel images as shown in Figure 1. We achieve this with a three-stage framework: 1) Conditioned on a target pose, we first estimate a person semantic layout to provide richer guidance to the generation process. 2) Built on two feature pyramid networks, a cascaded flow estimation network then accurately estimates the appearance matching between corresponding clothing regions. The resulting dense flow warps the source image to flexibly account for deformations. 3) Finally, a generative network takes the warped clothing regions as inputs and renders the target view. We conduct extensive experiments on the DeepFashion dataset for pose-guided person image generation and on the VITON dataset for the virtual try-on task. Strong qualitative and quantitative results validate the effectiveness of our method.
Ultrasonic technology for nonintrusive level measurement on commercial aircraft (2001)

Aircraft efficiency is improved by implementing more accurate fluid level measurement solutions. Reducing the amount of fuel or water needed during a flight reduces the weight and increases the aircraft efficiency. Airlines know how much water or fuel is necessary for a specific flight/aircraft combination. Sensors with improved accuracy allow the required level to be added, dependent on the passenger loading and flight plan. The development of a nonintrusive level sensor based on ultrasonic technology is discussed. Benefits of the sensor are high reliability, high accuracy, broad measurement range, light weight, low cost, and ease of installation.
Real-time monitoring of laser beam welding using infrared weld emissions (1998)

A nonintrusive, solid-state device has been developed to monitor in real time the infrared emissions during laser welding. The weld monitor output is an analog signal (100–1000 mV) that depends on the beam power and weld characteristics. The dc level of this signal is related to weld penetration, while ac portions of the output can be correlated with surface irregularities and part misalignment or contamination. Changes in dc behavior are also noted for both full and deep penetration welds. Full penetration welds are signified by an abrupt reduction in the weld monitor output. Bead-on-plate welds were made on steel, aluminum, and magnesium with both a cw CO2 laser and a pulsed Nd:YAG laser to explore the relationships between the weld characteristics and the weld monitor output.
Heterogeneity of cancer-initiating cells within glioblastoma (2012)

Malignant gliomas, particularly glioblastoma multiforme (GBM), account for the majority of brain tumors. Their incidence is increasing world wide and they are incurable. Although a transient response to therapy is observed, tumor recurrence is inevitable and occurs within tissue that has received cytotoxic therapy. This suggests that a subpopulation of resistant cells is responsible for tumor regrowth. The treatment of GBMs represents a daunting challenge to clinicians due principally to the lack of effective therapeutic options. One explanation for this is the marked cellular and genetic heterogeneity within and across these types of tumors. Unravelling the cellular composition of gliomas and describing cell lineage relationships are essential for therapeutic breakthroughs. The recent proposal that a small percentage of cells with stem cells characteristics are responsible for tumor initiation and growth has sparked an interest in applying approaches used to study somatic stem cells toward an understanding of the cellular elements responsible for cancer progression and recurrence. To outline the relevance of these findings is the purpose of this review.
Preventing neonatal herpes: Protection after maternal mRNA-lipid nanoparticle vaccination equals or exceeds that from prior maternal genital infection in murine models (2022)

Abstract Neonates are vulnerable to poor outcomes from herpes simplex virus (HSV), but maternal infection eliciting non-sterilizing immunity prior to delivery provides some protection. We are therefore pursuing birthing parent vaccination with nucleoside-modified mRNA-lipid nanoparticles (mRNA-LNPs) to prevent neonatal herpes. Our trivalent vaccine encodes HSV type 2 (HSV-2) proteins involved in attachment and immune evasion (gC2, gD2, and gE2). We have shown that this vaccine protects against genital HSV and neonatal HSV-2 in mice. Here, we tested how well murine dam vaccination prior to pregnancy protects pups against HSV type 1 (HSV-1) challenge compared to prior maternal intravaginal HSV-1 infection. Dams were immunized intramuscularly with the HSV-2 trivalent mRNA-LNP vaccine or a control immunogen (PolyC RNA-LNP), or were infected intravaginally with HSV-1 (HSV-1 ivag). Pups were challenged intranasally with HSV-1 on the 3rd day of life. Pups born to dams previously immunized or infected survived through 28 days (mRNA 95% vs. HSV-1 ivag 96%, N.S.; vs. PolyC 13%, p&lt;0.0001). Likewise, they were protected from HSV-1 dissemination to the brain, lung, liver, spleen, and kidney by plaque assay (mRNA 2/45 organs affected vs. HSV-1 ivag 6/20, p=0.0083; vs. PolyC 69/80, p&lt;0.0001). Additionally, fewer organs showed necrosis by histopathology of brain, lung, and liver, though not statistically significant, and fewer mRNA and HSV-1 ivag pups had detectable HSV-1 DNA in the trigeminal ganglia by qPCR (mRNA 6/30 vs. HSV-1 ivag 7/26, N.S.; vs. PolyC 17/18, p&lt;0.0001). These studies demonstrate that the mRNA vaccine provides comparable to improved protection against neonatal HSV-1 challenge versus prior maternal HSV-1 intravaginal infection. AD was supported by NIH NIAID T32 AI118684. HMF, SA, GHC, and DW were supported by NIH NIAID R01 AI139618 and an unrestricted grant from BioNTech SE. KPE was supported by NIH T32 NS007180. The funders had no input or influence on the content of this abstract.