gebhart/patrepeval huggingface dataset:
The High-Risk Human Papillomavirus Type 16 E6 Counters the GAP Function of E6TP1 toward Small Rap G Proteins (2002)

We have recently identified E6TP1 (E6-targeted protein 1) as a novel high-risk human papillomavirus type 16 (HPV16) E6-binding protein. Importantly, mutational analysis of E6 revealed a strong correlation between the transforming activity and its abilities to bind and target E6TP1 for ubiquitin-mediated degradation. As a region within E6TP1 has high homology with GAP domains of known and putative Rap GTPase-activating proteins (GAPs), these results raised the possibility that HPV E6 may alter the Rap small-G-protein signaling pathway. Using two different approaches, we now demonstrate that human E6TP1 exhibits GAP activity for Rap1 and Rap2, confirming recent findings that a closely related rat homologue exhibits Rap-specific GAP activity. Using mutational analysis, we localize the GAP activity to residues 240 to 945 of E6TP1. Significantly, we demonstrate that coexpression of HPV16 E6, by promoting the degradation of E6TP1, enhances the GTP loading of Rap. These results support a role of Rap small-G-protein pathway in E6-mediated oncogenesis.
POST-REDUCTION TREATMENT OF LIQUID PHASE METHANOL SYNTHESIS CATALYST USING CARBON DIOXIDE (1991)

ABSTRACT This work focuses on the investigation of the catalyst post-treatment in the liquid phase methanol synthesis process. The novel post-treatment process, using carbon dioxide, has been developed and experimentally proven to be effective not only in maintaining the initial catalytic activity over a long period of usage but also in improving the mechanical and chemical strength of the catalyst. It was also found that the role of ZnO in the catalytic reaction, if any, can be nicely replaced by ZnCO3.
<title>Embedded triboluminescent structural damage sensors</title> (2000)

Triboluminescent materials offer a viable route to real-time structural damage sensing. The sensors can be externally attached to the surface of metals or composites, or embedded within composites. In-situ monitoring of structural damage in composites is particularly relevant since severe internal damage can exist with little indication of this damage on the composite surface. The main issue related to embedding triboluminescent sensors within composites is how to access efficiently the optical signal generated upon structural damage and how to guide efficiently this optical damage signal to a remote detector. Earlier work relied on side-coupling of the triboluminescent light into a curved conventional silica fibre and/or end-coupling into the silica fibre, if the damage to the host structure had also broken the fibre. Both these light collection methods are, of course, inefficient and resulted in an optical damage signal with a very poor S/N ratio. By using novel photoluminescent polymeric and silica fibres, we have shown that it is possible to efficiently capture and guide the optical damage signal from an embedded triboluminescent sensor to a remote detector. These fibres resulted in a signal with a S/N ratio that was orders of magnitude larger than that achieved using the conventional silica fibre. Futhermore, we have shown that the light collection/guiding technique can be used effectively with triboluminescent sensors embedded within black CFRP composites. Finally, we have demonstrated that for resins and GFRP composites, triboluminescent sensors act as truly global damage sensors, wehreas for CFRP composites, the damage sensing is localised to areas close to the embedded photoluminescent fibre.
Multi-scale study of the deformation mechanisms of thermoelectric p-type half-Heusler Hf0.44Zr0.44Ti0.12CoSb0.8Sn0.2 (2018)

Increasing the figure of merit ZT of thermoelectric (TE) alloys is a challenge that is currently attempted through various metallurgy methods, including nanostructuring and dislocation engineering. Microstructures with such a level of complexity raise questions about the mechanical reliability of these new materials. Indeed, despite the values of hardness and elastic modulus known for the clear majority of TE materials, the data on deformation mechanisms are still rare. Focusing on the nanostructured p-type half-Heusler Hf0.44Zr0.44Ti0.12CoSb0.8Sn0.2, our multi-scale study aims to analyze the deformation mechanisms. Experiments conducted at macro-, meso-, and micro-scale are designed to trigger and assess plasticity mechanisms. Compression testing on bulk samples subject to a confining pressure environment and temperature leads to an exclusive brittle failure. The mixed-mode failure mechanisms involve switching between intra- and inter-granular crack propagation, depending on the grain size met by the crack tip. Cube-corner nanoindentation at meso-scale generates cracks and enables fracture toughness estimation, while TEM analysis of the crack tip area confirms no dislocation activity and 3D-Electron Back Scattered Diffraction technique confirms the mixed crack propagation behavior. At micro-scale, micro-pillar compression stress-strain curves and failure mechanisms are comparable with bulk samples testing analysis. These results can be used to provide design guidelines for more crack-resistant TE alloys.
Development of a pulsed backscatter-absorption gas-imaging system and its application to the visualization of natural gas leaks (1998)

The design and evaluation of a backscatter-absorption gas-imaging sensor that operates in a pulsed mode is described. It is capable of video visualization of natural gas leaks. Its development was motivated by the need for a methane imaging system to operate at ranges and sensitivities useful to the natural gas industry. The imager employs pulsed laser illumination at a repetition rate of 30 Hz and an average power of ~150 mW to image gas at standoff ranges of as long as 100 m, using a backscatter target with a reflectivity of 0.016 sr(-1). This is a tenfold improvement over an earlier raster-scanned imager. Natural gas leaks as small as 1.6 x 10(-4) standard liters/s [equal to 0.02 standard cubic feet per hour (scfh)] were imaged at short ranges; leaks as low as 7.9 x 10(-4) standard liters/s (0.1 scfh) were observed at long ranges. Data are compared with model predictions, and potential extensions to a fieldable prototype are discussed. The optimization of a direct-injection focal-plane array for detecting short (nanosecond) laser pulses is described.
Human cytidine deaminase: purification of enzyme, cloning, and expression of its complementary DNA. (1994)

Cytidine (CR) deaminase was purified 47,000-fold to homogeneity from human placenta. The molecular mass of CR deaminase was estimated to be 48.7 kDa by gel filtration and 16.1 kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis, suggesting that it contains three or four identical subunits. We determined the amino acid sequence of several peptide fragments and designed 5'-primers to amplify, by the polymerase chain reaction, a specific 364-base pair DNA fragment using human liver complementary DNA (cDNA) as the template. This DNA fragment, which contains the codons of one peptide, was used as a probe to screen a cDNA library from human liver. We isolated and sequenced a cDNA clone of 910 base pairs that contained a 5' nontranslated region, a 438-base pair coding region, and a 3' nontranslated region with a polyadenylated tail. The translated region of the clone contained a deduced sequence of 146 amino acids, with a predicted molecular mass of 16.2 kDa and the sequences of our peptides. The cDNA was ligated in pGEX vector and expressed in Escherichia coli. The expressed protein had a high CR deaminase activity and molecular mass of 16.3 kDa. These data demonstrate clearly that the open reading frame of our cDNA clone codes for a functional human CR deaminase. Polymerase chain reaction amplifications of gene-specific DNA fragments from human/rodent hybrid cells indicate the localization of CR deaminase gene to human chromosome 1. The cDNA for CR deaminase will be a useful molecular probe to investigate the importance of this enzyme in chemotherapy.
A hybrid electronically scanned pressure module for cryogenic environments (1995)

Pressure is one of the most important parameters measured when testing models in wind tunnels. For models tested in the cryogenic environment of the National Transonic Facility at NASA Langley Research Center, the technique of utilizing commercially available multichannel pressure modules inside the models is difficult due to the small internal volume of the models and the requirement of keeping the pressure transducer modules within an acceptable temperature range well above the -173 degrees C tunnel temperature. A prototype multichannel pressure transducer module has been designed and fabricated with stable, repeatable sensors and materials optimized for reliable performance in the cryogenic environment. The module has 16 single crystal silicon piezoresistive pressure sensors electrostatically bonded to a metalized Pyrex substrate for sensing the wind tunnel model pressures. An integral temperature sensor mounted on each silicon micromachined pressure sensor senses real-time temperature fluctuations to within 0.1 degrees C to correct for thermally induced non-random sensor drift. The data presented here are from a prototype sensor module tested in the 0.3 M cryogenic tunnel and thermal equilibrium conditions in an environmental chamber which approximates the thermal environment (-173 degrees C to +60 degrees C) of the National Transonic Facility.
Complementation of apolipoprotein B mRNA editing by human liver accompanied by secretion of apolipoprotein B48. (1994)

Mammalian small intestine secretes a truncated apolipoprotein B (apoB48) species as a result of tissue-specific post-transcriptional RNA editing. The human liver, by contrast, contains only unedited apoB mRNA and secretes only apoB100. We have recently isolated a cDNA clone from rat small intestine which encodes an apoB mRNA editing protein, REPR (Teng, B., Burant, C.F., and Davidson, N.O. (1993) Science 260, 1816-1819). The current study demonstrates that homogenates of Xenopus oocytes expressing REPR confer editing ability upon S100 extracts prepared from human liver when tested on a synthetic apoB RNA template in vitro. Transfection of REPR into HepG2 cells resulted in editing of endogenous apoB mRNA and the appearance of an apoB48-like protein in the media. Extracts prepared from these transfected cells edit mammalian apoB RNA templates when incubated alone and with enhanced efficiency in the presence of chicken intestinal S100 extracts. The results suggest that human liver expresses factor(s) which are critical to apoB mRNA editing and which allow functional complementation of REPR in vivo.
Using labeled paths for loop-free on-demand routing in ad hoc networks (2004)

We present the Feasible Label Routing (FLR) protocol for mobilead hoc networks, which uses path information to establish routes to destinations on demand. FLR enables loop-free incremental(hop-by-hop) routing of data packets using only the addresses of their destinations. Like the dynamic source routing (DSR) protocol, FLR avoids the need for any time-stamps or sequence numbers by the use of path vectors exchanged when routes are established or repaired. Instantaneous loop freedom is attained by using path information for a destination as labels with which routers are ordered lexicographically with respect to the destination, i.e., FLR ensures that the labels of routers for a given destination become "smaller" the closer they are to the destination. Simulation experiments in Qualnet show that the performance of FLR is far better than the performance of the ad-hoc on-demand distance vector (AODV) protocol, the dynamic source routing (DSR) protocol, and the optimized link state routing (OLSR) protocol, in terms of the packet delivery ratio and average delivery latencies achieved, as well as the overhead incurred in the network.
Microbial Production of Optically Active 1,3-Butanediol from 4-Hydroxy-2-butanone (1993)

Journal Article Microbial Production of Optically Active 1,3-Butanediol from 4-Hydroxy-2-butanone Get access Akinobu Matsuyama, Akinobu Matsuyama Daicel Chemical Industries Ltd., Arai Plant, 230 Arai, Arai-shi, Niigata 944, Japan Search for other works by this author on: Oxford Academic Google Scholar Yoshinori Kobayashi, Yoshinori Kobayashi Daicel Chemical Industries Ltd., Arai Plant, 230 Arai, Arai-shi, Niigata 944, Japan Search for other works by this author on: Oxford Academic Google Scholar Hajime Ohnishi Hajime Ohnishi Daicel Chemical Industries Ltd., Arai Plant, 230 Arai, Arai-shi, Niigata 944, Japan Search for other works by this author on: Oxford Academic Google Scholar Bioscience, Biotechnology, and Biochemistry, Volume 57, Issue 2, 1 January 1993, Pages 348–349, https://doi.org/10.1271/bbb.57.348 Published: 01 January 1993 Article history Received: 20 August 1992 Published: 01 January 1993
6.1 W cw power, Al-free active region diode lasers at 0.805 μm (2002)

100 /spl mu/m-wide, 1 mm-long, 0.805 /spl mu/m-emitting, Al-free active region diode lasers with 10%/90% AR/HR facet coatings provide 6.1 W cw power at a heat sink temperature of T=10/spl deg/C. The corresponding internal power density at COMD, P~/sub COMD/, is /spl sim/15 MW/cm/sup 2/; almost twice the P~/sub COMD/ value, /spl sim/8 MW/cm/sup 2/, for similarly designed AlGaAs based devices.
Leveraging mmWave Imaging and Communications for Simultaneous\n Localization and Mapping (2018)

In this work, we propose a novel approach for high accuracy user localization\nby merging tools from both millimeter wave (mmWave) imaging and communications.\nThe key idea of the proposed solution is to leverage mmWave imaging to\nconstruct a high-resolution 3D image of the line-of-sight (LOS) and\nnon-line-of-sight (NLOS) objects in the environment at one antenna array. Then,\nuplink pilot signaling with the user is used to estimate the angle-of-arrival\nand time-of-arrival of the dominant channel paths. By projecting the AoA and\nToA information on the 3D mmWave images of the environment, the proposed\nsolution can locate the user with a sub-centimeter accuracy. This approach has\nseveral gains. First, it allows accurate simultaneous localization and mapping\n(SLAM) from a single standpoint, i.e., using only one antenna array. Second, it\ndoes not require any prior knowledge of the surrounding environment. Third, it\ncan locate NLOS users, even if their signals experience more than one\nreflection and without requiring an antenna array at the user. The approach is\nevaluated using a hardware setup and its ability to provide sub-centimeter\nlocalization accuracy is shown.\n
Fast Approximate $K$-Means via Cluster Closures (2013)

$K$-means, a simple and effective clustering algorithm, is one of the most widely used algorithms in multimedia and computer vision community. Traditional $k$-means is an iterative algorithm---in each iteration new cluster centers are computed and each data point is re-assigned to its nearest center. The cluster re-assignment step becomes prohibitively expensive when the number of data points and cluster centers are large. In this paper, we propose a novel approximate $k$-means algorithm to greatly reduce the computational complexity in the assignment step. Our approach is motivated by the observation that most active points changing their cluster assignments at each iteration are located on or near cluster boundaries. The idea is to efficiently identify those active points by pre-assembling the data into groups of neighboring points using multiple random spatial partition trees, and to use the neighborhood information to construct a closure for each cluster, in such a way only a small number of cluster candidates need to be considered when assigning a data point to its nearest cluster. Using complexity analysis, image data clustering, and applications to image retrieval, we show that our approach out-performs state-of-the-art approximate $k$-means algorithms in terms of clustering quality and efficiency.
ERCC1 gene polymorphism as a predictor for clinical outcome in advanced colorectal cancer patients treated with platinum-based chemotherapy. (2003)

Excision repair cross-complementation group 1 (ERCC1) is a highly conserved protein and an essential member of the nucleotide excision repair pathway. DNA repair is an important mechanism for resistance to platinum-based chemotherapy. Previous studies have shown that ERCC1 gene expression is associated with clinical outcome to platinum chemotherapy in a variety of cancers. Recently, 2 common polymorphisms in the ERCC1 gene have been described. The first is a single nucleotide polymorphism at codon 188 of the ERCC1 gene that causes a C-->T change but codes for the same amino acid, asparagine. This polymorphism may be associated with differential ERCC1 gene expression. The second is also a signal nucleotide polymorphism in the 3'-untranslated region and may affect MRNA stability. We hypothesized that these 2 polymorphisms may be associated with clinical outcome to platinum-based chemotherapy. We assessed the relationship between these ERCC1 gene polymorphism and clinical outcome to platinum-based chemotherapy in 106 patients with advanced refractory colorectal cancer. We found a significant associated between the ERCC1 codon 118 polymorphism and clinical outcome. Patients with the C/C genotype had a median survival of 15.3 months (95% CI, 6.0-12.1) and 11.1 months (95% CI, 5.8-16.2) for those with C/T and T/T genotypes, respectively. The ERCC1 codon 118 polymorphism may be a useful predictor of clinical outcome in advanced colorectal cancer patients treated with platinum-based chemotherapy.
<title>Liquid crystal polymers and networks for display applications</title> (2000)

Photopolymerization of liquid-crystalline (LC) monomers produces polymer films with a spatial control over the molecular organization. A powerful tool for creating even more complex molecular architectures than by LC order alone is photo-induced diffusion during polymerization of these monomers. Photo-induced diffusion during polymerization of chiral-nematic monomers yields a cholesteric network in which the helical pitch gradually changes over the cross-section of the film. The polarization selective reflection band can thus be made much wider than those of single pitch materials and may expand the whole visible spectrum. Performing photo- induced diffusion on a length-scale of half the cholesteric pitch by using a liquid-crystalline photoinitiator yields a cholesteric network with a deformed helix. Helix deformation gives higher order reflections and a built-in optical retardation. When the deformed helix is combined with a pitch gradient over the film thickness, the built-in retardation can be used for wide-band cholesteric polarizers that directly generate linearly polarized light without an additional quarter-wave foil.
The Mechanical Properties of Vulcanized Deproteinized Natural Rubber (2013)

Properties of deproteinized natural rubber (DPNR) were investigated in order to apply the DPNR latex as non-allergy vulcanized rubber thin film products. Removal of proteins from natural rubber was made by incubation of high ammonium natural rubber latex (HA-NR) with urea 0.1 wt%, 1 hour in the presence of surfactant at room temperature (DPNR). The nitrogen content of the DPNR was reduced to 0.05 wt%, which was lower than 0.45 wt% of HA-NR under the test condition. The reduction of protein content can be confirmed by observation a chemical structure through FT-IR technique. It is suggesting that the urea treatment is an effective method to remove protein from rubber latex. Then, physical properties of the DPNR latex were investigated. Amount of water soluble protein was observed through modified lowry method and it was found that water soluble protein content after incubation HA-NR with urea was reduced to 15 g/g (DPNR) from 4,245 g/g (HA-NR). It was found that VFA number of DPNR was lower than that of HA-NR due to lower amount of protein presence in rubber latex. The particle size and charge of rubber latex was examined by dynamic light scattering and zeta potential to compare between un-vulcanized latex and vulcanized latex. The mechanical properties were then observed when we applied the DPNR latex to vulcanized rubber glove.
A CMOS Bandpass Filter for Low-IF (2006)

A CMOS fully integrated 12th-order bandpass filter for low interemdiate frequency Bluetooth receivers is presented. The design is optimized to meet the selectivity and dynamic range requirements of Bluetooth while consuming relatively low power. The filter is based on unity gain cells and utilizes linearized MOSFET resistors for tuning. It exhibits a bandwidth of 1 MHz and a programmable center frequency range of 2 to 4 MHz. Experimental results obtained from a standard 0.5- m CMOS chip show that the filter exhibits an in-band dynamic range of 53.3 dB at gain of 0 dB, and 52 dB at gain of 15 dB, while consuming a total current of 1.32 mA. Attenuations of more than 10, 38, and 55 dB, are achieved for blockers one, two, and three, respectively. Index Terms—Bluetooth (BT) receivers, CMOS analog inte- grated circuits (ICs), current mode circuits, intermediate-fre- quency (IF) bandpass filters.
<title>Self-describing schemes for interoperable MPEG-7 multimedia content descriptions</title> (1998)

In this paper, we present the self-describing schemes for interoperable image/video content descriptions, which are being developed as part of our proposal to the MPEG-7 standard. MPEG-7 aims to standardize content descriptions for multimedia data. The objective of this standard is to facilitate content-focused applications like multimedia searching, filtering, browsing, and summarization. To ensure maximum interoperability and flexibility, our descriptions are defined using the eXtensible Markup Language (XML), developed by the World Wide Web Consortium. We demonstrate the feasibility and efficiency of our self-describing schemes in our MPEG-7 testbed. First, we show how our scheme can accommodate image and video descriptions that are generated by a wide variety of systems. Then, we present two systems being developed that are enabled and enhanced by the proposed approach for multimedia content descriptions. The first system is an intelligent search engine with an associated expressive query interface. The second system is a new version of MetaSEEk, a metasearch system for mediation among multiple search engines for audio-visual information.