gebhart/patrepeval huggingface dataset:
Immunogenicity of poliovirus B and T cell epitopes presented by hybrid porcine parvovirus particles (1995)

We have analysed the potential capacity of hybrid porcine parvovirus (PPV) capsids to present foreign epitopes to the immune system. Foreign sequences were introduced into the N and C termini of PPV VP2, which was previously shown to assemble spontaneously into parvovirus-like particles. The integrity of the C terminus was shown to be essential for preserving the structure of the capsid and therefore could not be used for epitope fusion. In contrast, insertion of sequences corresponding to T and B cell poliovirus epitopes in the N terminus did not alter the formation of particles. Moreover, the chimeric capsids containing the C3:T epitope were able to induce a T cell response in vivo. However, hybrid particles containing the C3:B epitope fused to the N terminus did not induce any peptide-specific antibody response, suggesting that the inserted B cell epitope was not exposed at the surface of the particles. These results show that the N terminus in PPV empty capsids is not an adequate site for insertion of B cell epitopes, but may be useful for T cell epitope presentation and suggest that the N terminus is located in an internal position.
Low-energy broad area electron beam for etching microelectronic materials (1987)

We have generated beams with electron current densities of more than 10 mA/cm2 at energies between 150 and 900 eV using a plasma source. This electron source can be operated at background pressures between 0.01 and 0.1 Torr in helium and with the addition of various gases, producing electron beams up to 3.8 cm in diameter. The broad area beam can be used to induce gas phase and surface reactions and has been successfully used to achieve anisotropic etching of SiO2 films in the reactant gas CF4.
An autozeroing floating-gate amplifier (2001)

We have developed a bandpass floating-gate amplifier that uses tunneling and pFET hot-electron injection to set its dc operating point adaptively. Because the hot-electron injection is an inherent part of the pFET's behavior, we obtain this adaptation with no additional circuitry. Because the gate currents are small, the circuit exhibits a high-pass characteristic with a cutoff frequency less than 1 Hz. The high-frequency cutoff is controlled electronically, as is done in continuous-time filters. We have derived analytical models that completely characterize the amplifier and that are in good agreement with experimental data for a wide range of operating conditions and input waveforms. This autozeroing floating-gate amplifier demonstrates how to use continuous-time floating-gate adaptation in amplifier design.
A self-synchronized RF-interconnect for 3-dimensional integrated circuits (2004)

A self-synchronized RF-interconnect technology (SSRFI), based on capacitor coupling and peak signal detection, is presented in this paper. It can be easily implemented in 3-dimensional ICs (3D-ICs) with small coupling capacitors (60 fF) to interconnect vertical active layers. The demonstrated SSRFI system, including both transmitter and receiver, has been designed, fabricated and verified in UMC 0.18 /spl mu/m CMOS with a verified PRBS (Pseudo Random Binary Sequence) data rate of 3 Gbit/s and a BER (Bit Error Rate) of 1.2/spl times/10/sup -10/. The core circuit burns about 4 mW from a 1.8 V supply and occupies 0.02 mm/sup 2/ chip area.
Micromachined capacitive ultrasonic immersion transducer for medical imaging (2002)

Piezoceramics have been the dominant transducer technology in ultrasound medical imaging for several decades. Recent progress in surface micromachined capacitive ultrasonic immersion transducers makes them an alternative transducer technology, especially in highly integrated two-dimensional arrays. This paper demonstrates that the surface micromachined capacitive ultrasonic immersion transducer performs at a level competitive enough to challenge the established piezoelectric transducers. Single element transducers and a variety of array transducers are fabricated with CMOS compatible micromachining technology. The transducers are observed to operate from 2 MHz to 15 MHz in immersion operation. Better than 100 dB dynamic range is evident around 4.5 MHz for a single device with only 6 dB of unknown return loss. Similar performance is observed in a pulse echo experiment. The transducer's beam pattern indicates that the device behaves as a uniform piston transducer. Theoretical analysis shows that it is feasible to build an ideal immersion transducer with 100% of bandwidth and 3 dB insertion loss in wide frequency ranges. The study in this paper concludes that micromachined ultrasonic transducers are an attractive alternative to piezoelectric transducers in ultrasound medical imaging.
Organosilicon Polymers as Precursors for Silicon Containing Ceramics: Recent Developments. (1987)

Abstract : A review of the dehydrocyclodimerization route to preceramic polysilazanes starting from (CH3SiHNH)n, the ammonolysis product of CH3SiHC12, as well as similar processing of coammonolysis products and the application of the living polymer intermediates involved in this chemistry in the preparation of novel hybrid preceramic polymers. Keywords: Silicon nitride, Polysilazanes, Silicon carbide, Ceramics.
New Approach for Gas Phase Synthesis and Growth Mechanism of MoS2 Fullerene-like Nanoparticles (2002)

Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter Facebook Reddit LinkedIn Tools Icon Tools Reprints and Permissions Cite Icon Cite Search Site Citation Alla Zak, Yishay Feldman, Vladimir Alperovich, Rita Rosentsveig, Reshef Tenne; New Approach for Gas Phase Synthesis and Growth Mechanism of MoS2 Fullerene‐like Nanoparticles. AIP Conf. Proc. 14 October 2002; 633 (1): 207–210. https://doi.org/10.1063/1.1514107 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAIP Publishing PortfolioAIP Conference Proceedings Search Advanced Search |Citation Search
FlatStore: An Efficient Log-Structured Key-Value Storage Engine for Persistent Memory (2020)

Emerging hardware like persistent memory (PM) and high-speed NICs are promising to build efficient key-value stores. However, we observe that the small-sized access pattern in key-value stores doesn't match with the persistence granularity in PMs, leaving the PM bandwidth underutilized. This paper proposes an efficient PM-based key-value storage engine named FlatStore. Specifically, it decouples the role of a KV store into a persistent log structure for efficient storage and a volatile index for fast indexing. Upon it, FlatStore further incorporates two techniques: 1) compacted log format to maximize the batching opportunity in the log; 2) pipelined horizontal batching to steal log entries from other cores when creating a batch, thus delivering low-latency and high-throughput performance. We implement FlatStore with the volatile index of both a hash table and Masstree. We deploy FlatStore on Optane DC Persistent Memory, and our experiments show that FlatStore achieves up to 35 Mops/s with a single server node, 2.5 - 6.3 times faster than existing systems.
UWB Array Design Using Variable Zo Technology and Central Force Optimization (2011)

This note applies Variable Zo technology to the design of an Ultra Wideband (UWB) Yagi-Uda array optimized using Central Force Optimization. Variable Zo is a novel and proprietary approach to antenna design and optimization that treats the feed system characteristic impedance, Zo, as a design variable instead of a fixed design parameter as is traditionally done. Variable Zo is applicable to any antenna design or optimization methodology, and using it will generally produce better antenna designs across any user-specified set of performance objectives.
Pulmonary Adenocarcinomas with Mutant Epidermal Growth Factor Receptors (2005)

To the Editor: Activating mutations of the DNA sequence encoding the catalytic kinase domain of the epidermal growth factor receptor (EGFR) have been shown to underlie responsiveness to gefitinib i...
Performance of combined OCT/MFI microendoscope for ovarian cancer detection (2018)

To improve early detection of ovarian cancer, we have designed and built a microendoscope that combines optical coherence tomography (OCT) and multispectral fluorescence imaging (MFI) into a 0.7 mm diameter package. An endoscope of this size allows access to the ovaries through the fallopian tubes creating a minimally invasive procedure. We characterize the falloposcope’s imaging behavior and show that this system provides contrast on ex vivo surgical samples of ovary and fallopian tube. In addition, we show the mechanical performance of the endoscope in an anatomically correct model of the female reproductive treatment.
Towards Generation of mJ-Level Ultrashort THz Pulses by Optical Rectification (2012)

The so far highest THz pulse energy (125 µJ) and efficiency (0.25%) were measured by optical rectification of 1.3 ps pulses in LiNbO3. The generation of mJ-level THz pulses is predicted by calculations.
Peptides Containing Membrane-transiting Motifs Inhibit Virus Entry (2002)

Several exceptional peptides have been identified that can cross plasma membranes and deliver various covalently linked moieties into cells. We report the surprising observation that each of four structurally distinct transiting peptides tested displayed antiviral activity and inhibited herpes simplex virus entry into cells. All four peptides inhibited infection at concentrations in the low micromolar range. Some of the peptides selectively and reversibly blocked entry without inactivating virions in a persistent manner. For other peptides, the effects on virus entry were not readily distinguishable from virus inactivation. High concentrations of nearly all peptides lead to irreversible inactivation of virions. By various criteria, the peptides differed in their ability to inactivate virions and in the temperature dependence of inactivation. Testing of peptides with modifications known to disrupt transport revealed that, in some instances, transport activity did not correlate with antiviral activity. These results identify inhibition of viral entry as another common property of membrane-transiting peptides in addition to their ability to cross membranes and transport materials into cells. These or related peptides may be useful as agents to prevent infection and to study the process of viral entry.
A Three-Arm Scaffold Carrying Affinity Molecules for Multiplex Recognition Imaging by Atomic Force Microscopy: The Synthesis, Attachment to Silicon Tips, and Detection of Proteins (2015)

We have developed a multiplex imaging method for detection of proteins using atomic force microscopy (AFM), which we call multiplex recognition imaging (mRI). AFM has been harnessed to identify protein using a tip functionalized with an affinity molecule at a single molecule level. However, many events in biochemistry require identification of colocated factors simultaneously, and this is not possible with only one type of affinity molecule on an AFM tip. To enable AFM detection of multiple analytes, we designed a recognition head made from conjugating two different affinity molecules to a three-arm linker. When it is attached to an AFM tip, the recognition head would allow the affinity molecules to function in concert. In the present study, we synthesized two recognition heads: one was composed of two nucleic acid aptamers, and the other one composed of an aptamer and a cyclic peptide. They were attached to AFM tips through a catalyst-free click reaction. Our imaging results show that each affinity unit in the recognition head can recognize its respective cognate in an AFM scanning process independently and specifically. The AFM method was sensitive, only requiring 2 to 3 μL of protein solution with a concentration of ∼2 ng/mL for the detection with our current setup. When a mixed sample was deposited on a surface, the ratio of proteins could be determined by counting numbers of the analytes. Thus, this mRI approach has the potential to be used as a label-free system for detection of low-abundance protein biomarkers.
Efficient compensation of inter-channel nonlinear effects via digital backward propagation in WDM optical transmission (2010)

An advanced split-step method is employed for the digital backward-propagation (DBP) method using the coupled nonlinear Schrodinger equations for the compensation of inter-channel nonlinearities. Compared to the conventional DBP, cross-phase modulation (XPM) can be efficiently compensated by including the effect of the inter-channel walk-off in the nonlinear step of the split-step method (SSM). While self-phase modulation (SPM) compensation is inefficient in WDM systems, XPM compensation is able to increase the transmission reach by a factor of 2.5 for 16-QAM-modulated signals. The advanced SSM significantly relaxes the step size requirements resulting in a factor of 4 reduction in computational load.
Multifunctional porous aramids (aerogels) by efficient reaction of carboxylic acids and isocyanates (2011)

Polymerization of trifunctional polyaromatic carboxylic acids and isocyanates in dilute DMF solutions using the rather underutilized reaction of the carboxylic acid group (–COOH) with isocyanates (–NCO) towards amides (–NH(CO)–) induces early phase separation of surface-active aramid nanoparticles that form a solvent-filled 3D network stabilized against collapse by the chemical energy of the interparticle covalent bridges (crosslinks). Those wet-gels can be dried with liquid CO2 taken out at the end as a supercritical fluid into lightweight (bulk density ∼0.3 g cm−3) highly porous (77% v/v) multifunctional materials classified as aerogels with high specific energy absorption (37 J g−1), open-air speed of sound (338 m s−1) and Styrofoam-like thermal conductivity (0.028 W m−1 K−1).
An efficient Hadoop data replication method design for heterogeneous clusters (2016)

The performance of each datanode in a heterogeneous Hadoop cluster differs, and the number of slots that can be numbered to simultaneously execute tasks differs. For this reason, Hadoop is susceptible to replica placement problems and data replication problems. Because of this, replication problems and allocation problems occur. These problems can deteriorate the performance of Hadoop. In this paper, we summarize existing research to improve data locality, and design a data replication method to solve replication and allocation problems.
A closed-form method for finding source locations from microphone-array time-decay estimates (2002)

The linear intersection (LI) estimator, a closed-form method for the localization of source positions given only the sensor array time-delay estimate information, is presented. The array is constrained to be composed of 4-element sub-arrays configured in 2 centered orthogonal pairs. A bearing line in 3-space is estimated from each sub-array and potential source locations are found via closest intersection of bearing line pairs. The final location estimate is determined by a probabilistic weighting of these potential locations. The LI estimator is shown to be robust and accurate, to closely model the ML estimator, and to outperform a representative algorithm. The computational complexity of the LI estimator is suitable for use in real-time microphone-array applications.
Optimum waveforms for differential ion mobility spectrometry (FAIMS) (2008)

Differential mobility spectrometry or field asymmetric waveform ion mobility spectrometry (FAIMS) is a new tool for separation and identification of gas-phase ions, particularly in conjunction with mass spectrometry. In FAIMS, ions are filtered by the difference between mobilities in gases (K) at high and low electric field intensity (E) using asymmetric waveforms. An infinite number of possible waveform profiles make maximizing the performance within engineering constraints a major issue for FAIMS technology refinement. Earlier optimizations assumed the non-constant component of mobility to scale as E(2), producing the same result for all ions. Here we show that the optimum profiles are defined by the full series expansion of K(E) that includes terms beyond the first that is proportional to E(2). For many ion/gas pairs, the first two terms have different signs, and the optimum profiles at sufficiently high E in FAIMS may differ substantially from those previously reported, improving the resolving power by up to 2.2 times. This situation arises for some ions in all FAIMS systems, but becomes more common in recent miniaturized devices that employ higher E. With realistic K(E) dependences, the maximum waveform amplitude is not necessarily optimum, and reducing it by up to approximately 20% to 30% is beneficial in some cases. The present findings are particularly relevant to targeted analyses where separation depends on the difference between K(E) functions for specific ions.
Internal paralleled active neutral point clamped converter with logic-based flying capacitor voltage balancing (2017)

An internal paralleled active neutral point clamped converter (IP-ANPC) is presented in this paper. Advantages of the IP-ANPC converter include modularity, reliability and efficiency improvement, capability of interleaving, and better utilization of wide band-gap (WBG) devices. To simplify the operation of the IP-ANPC converter and provide an easy interleaving solution, a logic-based flying capacitor voltage balancing scheme is proposed. The method is efficient and adaptive to all kinds of PWM strategies. Simulation results are presented to validate the proposed topology and balancing scheme.
5′–3′-UTR interactions regulate p53 mRNA translation and provide a target for modulating p53 induction after DNA damage (2010)

Optimal induction of p53 protein after DNA damage requires RPL26-mediated increases in p53 mRNA translation. We report here the existence of a dsRNA region containing complementary sequences of the 5'- and 3'-untranslated regions (UTRs) of human p53 mRNA that is critical for its translational regulation by RPL26. Mutating as few as 3 bases in either of the two complementary UTR sequences abrogates the ability of RPL26 to bind to p53 mRNA and stimulate p53 translation, while compensatory mutations restore this binding and regulation. Short, single-strand oligonucleotides that target this 5'-3'-UTR base-pairing region blunt the binding of RPL26 to p53 mRNA in cells and reduce p53 induction and p53-mediated cell death after several different types of DNA damage and cellular stress. The ability to reduce stress induction of p53 with oligonucleotides or other small molecules has numerous potential therapeutic uses.
Abstract A3: Enhanced effector responses in CAR-redirected CD8+ T cells deficient in diacylglycerol kinases. (2013)

Abstract The use of chimeric antigen receptor (CAR)-redirected CD8+ T cells in the treatment of malignancy is a novel strategy that has shown tremendous promise; however, it remains likely this strategy could be enhanced when combined with additional approaches. We hypothesized that deletion of signaling mediators that negatively regulate TCR signal transduction might enhance CAR signaling and function, since CARs utilize the same machinery that transduces TCR signals. We tested CAR activity and function in T cells that lacked one or both lymphocyte-specific isoforms of diacylglycerol kinase (dgk), proteins that metabolize the second messenger DAG and limit Ras/ERK activation. We found that primary murine T cells transduced with CARs specific for the human tumor antigen mesothelin demonstrated greatly enhanced cytokine production and cytotoxicity when co-cultured with a murine mesothelioma line that stably expresses mesothelin. Additionally, we found that dgk-deficient CAR transduced T cells were more effective in limiting the growth of implanted tumors, both concurrent with and after establishment of tumor. These results suggest that deletion of negative regulators of TCR signaling enhances the activity and function of CAR-expressing T cells, and identify dgks as a potential target for improving the clinical potential of CARs. Citation Format: Matthew Riese, Liang-Chuan Wang, Anjana Ranganathan, Gary Koretzky, Steven Albelda. Enhanced effector responses in CAR-redirected CD8+ T cells deficient in diacylglycerol kinases. [abstract]. In: Proceedings of the AACR Special Conference on Tumor Immunology: Multidisciplinary Science Driving Basic and Clinical Advances; Dec 2-5, 2012; Miami, FL. Philadelphia (PA): AACR; Cancer Res 2013;73(1 Suppl):Abstract nr A3.
A Defined O-Antigen Polysaccharide Mutant of<i>Francisella tularensis</i>Live Vaccine Strain Has Attenuated Virulence while Retaining Its Protective Capacity (2007)

Francisella tularensis, the causative agent of tularemia, has been designated a CDC category A select agent because of its low infective dose (<10 CFU), its ready transmission by aerosol, and its ability to produce severe morbidity and high mortality. The identification and characterization of this organism's virulence determinants will facilitate the development of a safe and effective vaccine. We report that inactivation of the wbtA-encoded dehydratase of the O-antigen polysaccharide (O-PS) locus of the still-unlicensed live vaccine strain of F. tularensis (LVS) results in a mutant (the LVS wbtA mutant) with remarkably attenuated virulence. Western blot analysis and immune electron microscopy studies associate this loss of virulence with a complete lack of surface O-PS expression. A likely mechanism for attenuation is shown to be the transformation from serum resistance in the wild-type strain to serum sensitivity in the mutant. Despite this significant attenuation in virulence, the LVS wbtA mutant remains immunogenic and confers protective immunity on mice against challenge with an otherwise lethal dose of either F. tularensis LVS or a fully virulent clinical isolate of F. tularensis type B. Recognition and characterization of the pivotal role of O-PS in the virulence of this intracellular bacterial pathogen may have broad implications for the creation of a safe and efficacious vaccine.
Induction of Cross-reactive Humoral Immune Response by Immunization with Mimotopes of the Hypervariable Region 1 of the Hepatitis C Virus (2001)

Hepatitis C Virus (HCV) is a major cause of chronic hepatitis, liver cirrhosis and hepatocellular carcinoma, worldwide, and the development of an effective vaccine represents a high priority goal. The Hyper Variable Region 1 (HVR1) of the second Envelope protein (E2) of HCV contains a principal neutralizing determinant, but it is highly variable among different isolates and it is involved in the escape from host immune response. Thus, to be effective, a vaccine should elicit a cross-reacting humoral response against the majority of viral variants. We show that it is possible to achieve a broadly cross-reactive immune response in rabbits by immunization with mimotopes of the HVR1. selected from a specialized phage library using HCV patients' sera. At least some of the cross-reacting anti-mimotope antibodies, elicited in rabbits, recognize discontinuous epitopes in a manner similar to those induced by the virus in infected patients.