gebhart/patrepeval huggingface dataset:
Memristor logic gates (2018)

There are numerous approaches to implementing logic with memristors. The most popularized approach is the IMPLY operation, most commonly known from HP Labs' groundbreaking study. However, since this study, numerous other approaches have been proposed, which offer differing properties and trade-offs. Some of these approaches include memristors-as-drivers (MAD) gates, hybridCMOS gates, MAGIC gates, threshold gates, Zhang, and others. This chapter will explore two of these approaches in detail: the IMPLY and MAD operations because they have the widest applicability and the most research in their use. The remaining approaches will be discussed briefly for completion.
Evaluation of a fluorescence feedback system for guidance of laser angioplasty (1995)

By discriminating atherosclerotic from normal tissue and by confirming catheter-tissue contact and saline hemodilution, fluorescence feedback should minimize irradiation of normal tissue and/or blood and enhance the safety and efficacy of laser angioplasty.
Improving Escherichia coli Alkaline Phosphatase Efficacy by Additional Mutations inside and outside the Catalytic Pocket (2001)

We describe a strategy that allowed us to confer on a bacterial (E. coli) alkaline phosphatase (AP) the high catalytic activity of the mammalian enzyme while maintaining its high thermostability. First, we identified mutations, at positions other than those occupied by essential catalytic residues, which inactivate the bacterial enzyme without destroying its overall conformation. We transferred concomitantly into the bacterial enzyme four residues of the mammalian enzyme, two being in the catalytic pocket and two being outside. Second, the gene encoding the inactive mutant was submitted to random mutagenesis. Enzyme activity was restored upon the single mutation D330N, at a position that is 12 A away from the center of the catalytic pocket. Third, this mutation was combined with other mutations previously reported to increase AP activity slightly in the presence of magnesium. As a result, at pH 10.0 the phosphatase activity of both mutants D330N/D153H and D330N/D153G was 17-fold higher than that of the wild-type AP. Strikingly, although the two individual mutations D153H and D153G destabilize the enzyme, the double mutant D330N/D153G remained highly stable (T(m)=87 degrees C). Moreover, when combining the phosphatase and transferase activities, the catalytic activity of the mutant D330N/D153G increased 40-fold (k(cat)=3200 s-1) relative to that of the wild-type enzyme (k(cat)=80 s-1). Due to the simultaneous increase in K(m), the resulting k(cat)/K(m) value was only increased by a factor of two. Therefore, a single mutation occurring outside a catalytic pocket can dramatically control not only the activity of an enzyme, but also its thermostability. Preliminary crystallographic data of a covalent D330N/D153G enzyme-phosphate complex show that the phosphate group has significantly moved away from the catalytic pocket, relative to its position in the structure of another mutant previously reported.
On Broadcast Disk Paging (2000)

Broadcast disks are an emerging paradigm for massive data dissemination. In a broadcast disk, data is divided into n equal-sized pages, and pages are broadcast in a round-robin fashion by a server. Broadcast disks are effective because many clients can simultaneously retrieve any transmitted data. Paging is used by the clients to improve performance, much as in virtual memory systems. However, paging on broadcast disks differs from virtual memory paging in at least two fundamental aspects: A page fault in the broadcast disk model has a variable cost that depends on the requested page as well as the current state of the broadcast. Prefetching is both natural and a provably essential mechanism for achieving significantly better competitive ratios in broadcast disk paging. In this paper, we design a deterministic algorithm that uses prefetching to achieve an O(n log k) competitive ratio for the broadcast disk paging problem, where k denotes the size of the client's cache. We also show a matching lower bound of $\Omega(n\log k)$ that applies even when the adversary is not allowed to use prefetching. In contrast, we show that when prefetching is not allowed, no deterministic online algorithm can achieve a competitive ratio better than $\Omega(nk)$. Moreover, we show a lower bound of $\Omega(n \log k)$ on the competitive ratio achievable by any nonprefetching randomized algorithm against an oblivious adversary. These lower bounds are trivially matched from above by known results about deterministic and randomized marking algorithms for paging. An interpretation of our results is that in the broadcast disk paging, prefetching is a perfect substitute for randomization.
Regulation of the glucocorticoid receptor via a BET-dependent enhancer drives antiandrogen resistance in prostate cancer (2017)

In prostate cancer, resistance to the antiandrogen enzalutamide (Enz) can occur through bypass of androgen receptor (AR) blockade by the glucocorticoid receptor (GR). In contrast to fixed genomic alterations, here we show that GR-mediated antiandrogen resistance is adaptive and reversible due to regulation of GR expression by a tissue-specific enhancer. GR expression is silenced in prostate cancer by a combination of AR binding and EZH2-mediated repression at the GR locus, but is restored in advanced prostate cancers upon reversion of both repressive signals. Remarkably, BET bromodomain inhibition resensitizes drug-resistant tumors to Enz by selectively impairing the GR signaling axis via this enhancer. In addition to revealing an underlying molecular mechanism of GR-driven drug resistance, these data suggest that inhibitors of broadly active chromatin-readers could have utility in nuanced clinical contexts of acquired drug resistance with a more favorable therapeutic index.
Activation of the LRP (lung resistance-related protein) gene by short-term exposure of human leukemia cells to phorbol ester and cytarabine. (1998)

Treatment-induced secondary drug resistance of tumor cells is a major cause of relapsed disease and therapeutic failure in cancer patients. It has been shown that the expression of the multidrug resistance MDR1/P-glycoprotein gene could be induced by short-term in vitro exposure of cells to protein kinase C (PKC) agonists or different chemotherapeutic drugs. We studied whether other genes involved in drug resistance are regulated by similar signaling pathways. Transient (up to 24 h) treatment of HL-60 or K562 leukemia cells with phorbol 12-myristate 13-acetate (TPA) resulted in increased steady-state level of LRP (lung resistance-related protein) mRNA and protein. Among conventional chemotherapeutic drugs tested, only cytarabine (Ara C) induced the LRP mRNA expression though no increase in LRP protein was detected. LRP gene activation was not detectable in either H9 T-cell leukemia or in solid carcinoma cell lines (BT-20, ZR-75-1, and SW 1573). None of the agents influenced the levels of MRP (multidrug resistance-associated protein) mRNA in any cell line tested. In HL-60 cells, the LRP activation by TPA or Ara C was sustained for at least 23 days after withdrawal of inducing agents. bis-Indolylmaleimide I, a potent PKC inhibitor, attenuated TPA-induced LRP activation. In contrast, the inhibitor had no effect on the LRP induction by Ara C. These data indicate that the LRP gene can be activated by different mechanisms, some of which involve PKC.
Nanocaged enzymes with enhanced catalytic activity and increased stability against protease digestion (2016)

Cells routinely compartmentalize enzymes for enhanced efficiency of their metabolic pathways. Here we report a general approach to construct DNA nanocaged enzymes for enhancing catalytic activity and stability. Nanocaged enzymes are realized by self-assembly into DNA nanocages with well-controlled stoichiometry and architecture that enabled a systematic study of the impact of both encapsulation and proximal polyanionic surfaces on a set of common metabolic enzymes. Activity assays at both bulk and single-molecule levels demonstrate increased substrate turnover numbers for DNA nanocage-encapsulated enzymes. Unexpectedly, we observe a significant inverse correlation between the size of a protein and its activity enhancement. This effect is consistent with a model wherein distal polyanionic surfaces of the nanocage enhance the stability of active enzyme conformations through the action of a strongly bound hydration layer. We further show that DNA nanocages protect encapsulated enzymes against proteases, demonstrating their practical utility in functional biomaterials and biotechnology.
Asexual Embryogenesis in Theobroma cacao L.1 (1979)

Abstract Cultured immature sexual embryos of Theobroma cacao proliferated asexual embryos, but embryogenesis was not observed from leaf, pericarp, or ovule tissues. Cotyledons from immature, unpigmented embryos (2.5 to 10 mm long) were highly embryognie, while larger more mature, pigmented cotyledons initiated roots as their only form of organized development. Embryogenesis occurred in light and darkness when a basal medium was supplemented with coconut water and 1.5 mg/liter NAA. Proliferated embryos recultured in a liquid medium continued development.
Nano size cerium hydroxide slurry for scratch-free CMP process (2014)

We report recent progress in a novel cerium hydroxide polishing slurry with particle size of about 5 nm nano size cerium hydroxide (NSC) abrasive. NSC has succeeded in omitting particles over 1 μm, and in reducing scratches to 1/30 of those with calcined ceria slurry. Even though, NSC maintains oxide removal rate similar to conventional ceria. Mixing NSC with the specific additives brought out tunable polishing of SiO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> , SiN, and poly-Si.
Human Retinal Progenitor Cell Transplantation Preserves Vision (2014)

Cell transplantation is a potential therapeutic strategy for retinal degenerative diseases involving the loss of photoreceptors. However, it faces challenges to clinical translation due to safety concerns and a limited supply of cells. Human retinal progenitor cells (hRPCs) from fetal neural retina are expandable in vitro and maintain an undifferentiated state. This study aimed to investigate the therapeutic potential of hRPCs transplanted into a Royal College of Surgeons (RCS) rat model of retinal degeneration. At 12 weeks, optokinetic response showed that hRPC-grafted eyes had significantly superior visual acuity compared with vehicle-treated eyes. Histological evaluation of outer nuclear layer (ONL) characteristics such as ONL thickness, spread distance, and cell count demonstrated a significantly greater preservation of the ONL in hRPC-treated eyes compared with both vehicle-treated and control eyes. The transplanted hRPCs arrested visual decline over time in the RCS rat and rescued retinal morphology, demonstrating their potential as a therapy for retinal diseases. We suggest that the preservation of visual acuity was likely achieved through host photoreceptor rescue. We found that hRPC transplantation into the subretinal space of RCS rats was well tolerated, with no adverse effects such as tumor formation noted at 12 weeks after treatment.
On collisionless energy absorption in plasmas: Theory and experiment in spherical geometry (2006)

An investigation of the rf impedance characteristics of a small spherical probe immersed in a laboratory plasma is ongoing in the large Space Physics Simulation Chamber [D. N. Walker et al., Rev. Sci. Instrum. 65, 661 (1994)] at the Naval Research Laboratory. The data taken are from network analyzer measurements of the reflection coefficient obtained when applying a low level rf signal to the probe which is either near floating potential or negatively dc biased in a low pressure plasma. As is well known, sheaths form around objects placed inside plasmas. The electron density is smaller inside the sheath, and the reduction in density alters the plasma impedance. Surprisingly, the impedance becomes “resistive,” even though the plasma is effectively collisionless, at frequencies below the bulk plasma frequency, thus leading to collisionless energy absorption. This behavior comes directly from Maxwell's equations along with the cold fluid equations. The solutions obtained indicate that this form of plasma resistance is inversely proportional to the plasma density gradient evaluated at the location where the plasma frequency is equal to the applied frequency. The sphere for this work is typically near plasma potential or biased more negatively into the ion collection regime. Applying a supplemental, negative dc bias to the sphere thickens the sheath and tends to raise its resistance as the density gradient is softened. Much of the earlier work in the area of collisionless resistance concentrated primarily on planar probes as opposed to the present work which is concerned with spheres. Interpreting the results is simpler for a sphere and the results obtained agree well with theory as described. For comparison to the theory we use only the S11 parameter outputs (or reflection coefficients) of the network analyzer in the experimental series. Significant energy absorption is observed at frequencies generally near one-half the plasma frequency. One result of this work is that the most efficient transfer of power to the plasma occurs not unexpectedly when there is impedance matching between input impedance and output (collisionless) impedance. This paper is an exposition of these ideas along with data results and a comparison to theory for the spherical probe which has not been published in this form.
Ultrahigh strain response of field-actuated elastomeric polymers (2000)

Extremely large strains were achieved with elastomeric polymer films that are subject to high electric fields. The films were coated on both sides with complaint electrode material. When voltage was applied, the film compressed in thickness and expanded in area. The strain response is dominated by the electrostatic forces produced by the charges on the compliant electrodes. Actuated strains up to 117% were demonstrated with silicone elastomers, and up to 215% with acrylic elastomers. A key to achieving these large strains is to introduce a high prestrain to the film. Specific energy densities were much greater than those of other field-actuated materials. Because the response is electrostatic in nature, the actuation mechanism is predicted to be fast. Response speeds in excess of 2000 Hz have ben demonstrated in silicones. Acrylic response speeds are more than an order of magnitude slower, although the reason for this difference is not yet known. Measurement of material viscoelastic and electrical properties predicts that high efficiencies (> 80%) may be achieved with efficient driver circuits. A variety of actuators, including electrooptical devices, diaphragm pumps, and muscle like linear actuators, have been demonstrated with these materials, suggesting that this technology is well suited to small-scale electromechanical devices and robots.
Distributed Brillouin sensor system based on offset locking of two distributed feedback lasers (2008)

An offset locking technique, which uses an external optical delay line to tune the distributed feedback (DFB) laser frequency and a proportional-integral-derivative (PID) controller to lock the tuned frequency, is proposed for the first time, to the best of our knowledge, in the distributed Brillouin sensor system. This method provides large tuning range (greater than 1 GHz), high tuning speed (less than 100 mus per frequency step), and frequency tuning is independent of the laser frequency and power. The two DFB lasers are phase locked at the Brillouin frequency using a hardware PID controller. Using this offset locking with optical delay line, we demonstrated a high signal-to-noise ratio of 32 dB, which allows 1 m spatial resolution and better than 0.6 MHz frequency measurement accuracy (equivalent to 0.5 degrees C temperature resolution or 8 microepsilon strain resolution) over kilometers sensing length. The bias of the electro-optic modulator is controlled by a lock-in amplifier to provide high temperature or strain measurement accuracy.
Automated Scoring of Picture-based Story Narration (2015)

This work investigates linguistically motivated features for automatically scoring a spoken picture-based narration task. Specifically, we build scoring models with features for story development, language use and task relevance of the response. Results show that combinations of these features outperform a baseline system that uses state of the art speechbased features, and that best results are obtained by combining the linguistic and speech features.
Recent advances in homogeneous nickel catalysis (2014)

Some of the most recent and significant developments in homogeneous nickel catalysis are reviewed, including nickel-mediated cross-coupling reactions and carbon–hydrogen bond activation reactions.
Dispersion-compensating fibers (2005)

This paper reviews properties and use of conventional single-mode dispersion-compensating fibers (DCFs). The quality of the dispersion compensation expressed as residual dispersion after compensation is treated. Properties of actual DCFs for discrete compensation including loss and nonlinear effects are discussed. Fiber design with special emphasis on design tradeoffs is considered. The Raman properties of DCFs and their use as a discrete Raman amplifier are also discussed. Finally, DCF for use in dispersion-managed cables (DMCs) is addressed, including optimum fiber design and comparison of different configurations.
System Maximum Power Tracking among distributed power sources (2014)

Presented herein is Uniform Input Voltage Distribution (UIVD) control for Distributed-Input Series-Output (DISO) converter power systems. The primary control objective of UIVD for DISO converters is to achieve grouped maximum power throughput from non-identical renewable power sources. Secondly, this paper features a revised Maximum Power Tracking (MPT) controller design developed for DISO configurations that facilitate simultaneous processing of distributed power flows. In earlier research, the distributed source peak powers are individually tracked by converters controlled by independent MPT controllers without UIVD. However, when distributed power sources have similar peak power voltages with an achievable tracking efficiency of greater than 96%, such independent MPT controllers are not necessary. By utilizing UIVD control, near-maximum use of available power is achieved using a single MPT controller. The resulting system and control architectures offer near-maximum power transfer with a lower parts count. Two DISO power converter bus architectures are described herein: one having a battery-dominated output voltage and the other with a regulated output voltage. Through computer simulation, both power architectures are validated for fault-tolerant grouped UIVD control.
Shadow Vision Enhancement Using Polarization Imaging (2006)

Shadow is omnipresent in natural scenes. Conventional imaging methodologies are less effective in detecting features in shadow regions. Polarization imaging exploits another dimension of light that increases detection sensitivity in shadow and reveals hidden features.
A Lentiviral Strategy for Highly Efficient Retrograde Gene Transfer by Pseudotyping with Fusion Envelope Glycoprotein (2010)

The lentiviral vector system based on human immunodeficiency virus type 1 (HIV-1) is used extensively in gene therapy trials of neurological and neurodegenerative diseases. Retrograde axonal transport of viral vectors offers a great advantage to the delivery of genes into neuronal cell bodies that are situated in regions distant from the injection site. Pseudotyping of HIV-1-based vectors with selective variants of rabies virus glycoprotein (RV-G) increases gene transfer via retrograde transport into the central nervous system. Because large-scale application for gene therapy trials requires high titer stocks of the vector, pseudotyping of a lentiviral vector that produces more efficient retrograde transport is needed. In the present study, we developed a novel vector system for highly efficient retrograde gene transfer by pseudotyping an HIV-1 vector with a fusion envelope glycoprotein (termed FuG-B) in which the cytoplasmic domain of RV-G was substituted by the corresponding part of vesicular stomatitis virus glycoprotein. The FuG-B pseudotype shifted the transducing property of the lentiviral vector and enhanced the retrograde transport-mediated gene transfer into different brain regions innervating the striatum with greater efficiency than that of the RV-G pseudotype in mice. In addition, injection of the FuG-B-pseudotyped vector into monkey striatum (caudate and putamen) allowed for highly efficient gene delivery into the nigrostriatal dopamine system, which is a major target for gene therapy of Parkinson's disease. Our strategy provides a powerful tool for the treatment of certain neurological and neurodegenerative diseases by promoting retrograde gene delivery via a lentiviral vector.