MP63-14 EIGHT GENE EXPRESSION CLASSIFIER FOR NON INVASIVE BLADDER CANCER SURVEILLANCE (2019)

You have accessJournal of UrologyBladder Cancer: Basic Research & Pathophysiology III (MP63)1 Apr 2019MP63-14 EIGHT GENE EXPRESSION CLASSIFIER FOR NON INVASIVE BLADDER CANCER SURVEILLANCE Ruth Montalbo*, Juan Jose Lozano, Laura Izquierdo, Mercedes Ingelmo-Torres, Maria Pilar Oria, Juan Palou, Antoine G. van der Heijden, Rafael Medina, Joerg Schmidbauer, Aleix Prat, Maria J. Ribal, Antonio Alcaraz, and Lourdes Mengual Ruth Montalbo*Ruth Montalbo* More articles by this author , Juan Jose LozanoJuan Jose Lozano More articles by this author , Laura IzquierdoLaura Izquierdo More articles by this author , Mercedes Ingelmo-TorresMercedes Ingelmo-Torres More articles by this author , Maria Pilar OriaMaria Pilar Oria More articles by this author , Juan PalouJuan Palou More articles by this author , Antoine G. van der HeijdenAntoine G. van der Heijden More articles by this author , Rafael MedinaRafael Medina More articles by this author , Joerg SchmidbauerJoerg Schmidbauer More articles by this author , Aleix PratAleix Prat More articles by this author , Maria J. RibalMaria J. Ribal More articles by this author , Antonio AlcarazAntonio Alcaraz More articles by this author , and Lourdes MengualLourdes Mengual More articles by this author View All Author Informationhttps://doi.org/10.1097/01.JU.0000556857.84811.caAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVES: To develop a high accuracy non-invasive bladder cancer (BC) surveillance test focusing on the detection of non high-risk non-muscle invasive BC (NMIBC) tumours. METHODS: A total of 597 urines from BC patients, controls and patients in follow up for BC (PFBC) were included. The study was divided into three phases. In the urinary biomarker discovery phase, 84 urines from BC and control patients were retrospectively included and analyzed by RNA sequencing. In the classifier development phase, a total of 132 selected genes from previous phase were evaluated by nCounter® in 214 prospectively collected urines from PFBC (98 with tumour). Then, a diagnostic classifier was generated by logistic regression. Finally, in the classifier validation phase, a multicentric and international cohort of 248 urine samples (134 BC and 114 non-recurrent PFBC) was used to validate classifier performance. RESULTS: A total of 521 genes were found differentially expressed between non high-risk NMIBC samples and all other groups (p<0.05). An 8-gene diagnostic classifier with an AUC of 0.893 was developed. Validation of the 8-gene classifier in an independent cohort of PFBC achieved an overall sensitivity (SN) and a negative predictive value (NPV) of 96% and 97%, respectively (AUC=0.823). Of note this accuracy was maintained in non high-risk NMIBC group (SN=94%; NPV=98%). CONCLUSIONS: This 8-gene expression classifier has high SN and NPV in a real clinical scenario (PFBC). The use of this classifier can reduce the number of follow up cystoscopies in PFBC, although assessing its final place in clinical setting is necessary. Source of Funding: none Barcelona, Spain; Nijmegen, Netherlands; Sevilla, Spain; Vienna, Austria; Barcelona, Spain© 2019 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 201Issue Supplement 4April 2019Page: e902-e902 Advertisement Copyright & Permissions© 2019 by American Urological Association Education and Research, Inc.MetricsAuthor Information Ruth Montalbo* More articles by this author Juan Jose Lozano More articles by this author Laura Izquierdo More articles by this author Mercedes Ingelmo-Torres More articles by this author Maria Pilar Oria More articles by this author Juan Palou More articles by this author Antoine G. van der Heijden More articles by this author Rafael Medina More articles by this author Joerg Schmidbauer More articles by this author Aleix Prat More articles by this author Maria J. Ribal More articles by this author Antonio Alcaraz More articles by this author Lourdes Mengual More articles by this author Expand All Advertisement PDF downloadLoading ...