gebhart/patrepeval huggingface dataset:
Generalized Divergence-Free Carrier Smoothing with Applications to Dual Frequency Differential GPS (2009)

This paper develops a “Generalized Divergence-Free Smoothing” (GDFS) framework for carrier phase smoothing of Global Positioning System (GPS) pseudorange measurements. This framework encompasses widely-known dual frequency smoothing techniques and shows there is a continuum of possible smoothing configurations. When incorporated into Differential GPS (DGPS) architectures, this GDFS approach can be exploited to achieve specific objectives, such as minimum pseudorange errors, ionosphere-free measurements, or integer ambiguity resolution. A flexible DGPS architecture based on GDFS processing is presented. The GDFS framework also provides a methodology for evaluating the performance of signal combinations for modernized Global Navigation Satellite Systems.
DRUG SENSITIVITY TEST FOR PRIMARY CULTURE OF HUMAN CANCER-CELLS USING COLLAGEN GEL EMBEDDED CULTURE AND IMAGE-ANALYSIS (1993)

A new drug sensitivity assay method using the collagen gel embedded culture and image analysis was developed. Human cancer cells cultured in collagen gels showed extremely high cloning efficiency and in vivo-like drug response. In addition, image analysis was used successfully for the first time to automatically discriminate cancer colonies from contaminating fibroblastic cells by taking advantage of the difference in shape between them after cultivation to determine the drug response of cultured cancer cells accurately and easily. A new approach for the practical use of the drug sensitivity test on human cancer cells is suggested.
Monitoring of Anoxic/Oxic Process for Nitrogen and Chemical Oxygen Demand Removal Using Fuzzy Neural Networks (2009)

In this paper, a software sensor based on a fuzzy neural network (FNN) approach was proposed for the real-time estimation of nutrient concentrations and overcoming the problem of delayed measurements. To improve the FNN performance, fuzzy subtractive clustering was used to identify the model's architecture and optimize the fuzzy rule; meanwhile, a split network structure, applied separately for anaerobic and aerobic conditions, was used with dynamic modeling methods, such as an auto-regressive model with exogenous inputs. The proposed methodology was applied to a bench-scale anoxic/oxic process for biological nitrogen removal. It was possible to partially overcome the extrapolation problem of FNNs with the aid of multi-way principal component analysis, because it has the ability to detect abnormal situations, which could generate extrapolation. Real-time estimation of chemical oxygen demand, nitrate, and ammonium concentrations based on the model was successfully carried out with the simple online information of the anoxic/oxic system.
Profiling the temperature distribution in AlGaN/GaN HEMTs with nanocrystalline diamond heat spreading layers (2012)

Reduced performance in Gallium Nitride (GaN) based high electron mobility transistors (HEMTs) as a result of self-heating has been well-documented. A new approach, termed “diamond-before-gate" is shown to improve the thermal budget of the deposition process and enables large area diamond without degrading the gate metal NCD capped devices had a 20% lower channel temperature at equivalent power dissipation.
Generalized twin-nonlinear two-box digital predistorter for GaN based LTE Doherty power amplifiers with strong memory effects (2013)

In this paper, a generalized twin-nonlinear two-box predistorter is proposed for the linearization of highly nonlinear Doherty power amplifiers exhibiting strong memory effects. The proposed predistorter is made of the cascade of a memoryless look-up table followed by a generalized memory polynomial function and thus can be seen as a two-box implementation of the generalized memory polynomial model. The generalized twin-nonlinear two-box predistorter is experimentally benchmarked against the generalized memory polynomial model. The linearization performances of both models when applied on a GaN based Doherty power amplifier driven by a 20MHz LTE signal, demonstrate the superiority of the proposed predistorter which achieves better linearity performance while requiring a lower number of coefficients. Indeed, an extra 5dB is obtained in the ACLR while the number of predistorter coefficients is reduced by more than 60%.
High Level of Spinosad Production in the Heterologous Host Saccharopolyspora erythraea (2016)

This work provides an innovative and promising way to improve the industrial production of spinosad. At the same time, it also describes a successful method of heterologous expression for target metabolites of interest by replacing large gene clusters.
Effect of Flow Parameter Variations on Full Coverage Film-Cooling Effectiveness for a Gas Turbine Blade (2007)

The effect of film cooling holes placed along the span of a fully-cooled high pressure turbine blade in a stationary, linear cascade on film cooling effectiveness is studied using the Pressure Sensitive Paint (PSP) technique. Effect of showerhead injection at the leading edge and the presence of compound angled, diffusing holes on the pressure and suction side are also examined. Six rows of compound angled shaped film cooling holes are provided on the pressure side while four such rows are provided on the suction side of the blade. The holes have a laidback and fan-shaped diffusing cross-section. Another three rows of cylindrical holes are drilled at a typical angle on the leading edge to capture the effect of showerhead film coolant injection. The film cooling hole arrangement simulates a typical film cooled blade design used in stage 1 rotor blades for gas turbines used for power generation. A typical blowing ratio is defined for each film hole row and tests are performed for 100%, 150% and 200% of this typical value. Tests are performed for inlet Mach numbers of 0.36 and 0.45 with corresponding exit Mach numbers of 0.51 and 0.68 respectively. The flow remains subsonic in the throat region for both Mach numbers. The corresponding free stream Reynolds number, based on the axial chord length and the exit velocity, are 1.3 million and 1.74 million respectively. Freestream turbulence intensity level at the cascade inlet is 6%. Results show that varying blowing ratios can have a significant impact on film-cooling effectiveness distribution. Large spanwise variations in effectiveness distributions are also observed. Similar distributions were observed for both Mach numbers.
Optical low coherence reflectometry with range extension>150 m (1995)

An extended range optical low coherence reflectometer using a resonant fibre cavity and a novel technique for alias identification has achieved a measurement range >150 m combined with a two-point spatial resolution of <200 µm.
Graphene oxide as surfactant sheets (2010)

Graphite oxide sheet, now referred to as graphene oxide (GO), is the product of chemical oxidation and exfoliation of graphite powders that was first synthesized over a century ago. Interest in this old material has resurged in recent years, especially after the discovery of graphene, as GO is considered a promising precursor for the bulk production of graphene-based materials. GO sheets are single atomic layers that can readily extend up to tens of microns in lateral dimension. Therefore, their structure bridges the typical length scales of both chemistry and materials science. GO can be viewed as an unconventional type of soft material as it carries the characteristics of polymers, colloids, membranes, and as highlighted in this review, amphiphiles. GO has long been considered hydrophilic due to its excellent water dispersity, however, our recent work revealed that GO sheets are actually amphiphilic with an edge-to-center distribution of hydrophilic and hydrophobic domains. Thus, GO can adhere to interfaces and lower interfacial energy, acting as surfactant. This new property insight helps to better understand GO’s solution properties which can inspire novel material assembly and processing methods such as for fabricating thin films with controllable microstructures and separating GO sheets of different sizes. In addition, GO can be used as a surfactant sheet to emulsify organic solvents with water and disperse insoluble materials such as graphite and carbon nanotubes (CNTs) in water, which opens up opportunities for creating functional hybrid materials of graphene and other π-conjugated systems.
Inflammation increases cells expressing ZSCAN4 and progenitor cell markers in the adult pancreas (2013)

We have recently identified the zinc finger and SCAN domain containing 4 (Zscan4), which is transiently expressed and regulates telomere elongation and genome stability in mouse embryonic stem (ES) cells. The aim of this study was to examine the expression of ZSCAN4 in the adult pancreas and elucidate the role of ZSCAN4 in tissue inflammation and subsequent regeneration. The expression of ZSCAN4 and other progenitor or differentiated cell markers in the human pancreas was immunohistochemically examined. Pancreas sections of alcoholic or autoimmune pancreatitis patients before and under maintenance corticosteroid treatment were used in this study. In the adult human pancreas a small number of ZSCAN4-positive (ZSCAN4⁺) cells are present among cells located in the islets of Langerhans, acini, ducts, and oval-shaped cells. These cells not only express differentiated cell markers for each compartment of the pancreas but also express other tissue stem/progenitor cell markers. Furthermore, the number of ZSCAN4⁺ cells dramatically increased in patients with chronic pancreatitis, especially in the pancreatic tissues of autoimmune pancreatitis actively regenerating under corticosteroid treatment. Interestingly, a number of ZSCAN4⁺ cells in the pancreas of autoimmune pancreatitis returned to the basal level after 1 yr of maintenance corticosteroid treatment. In conclusion, coexpression of progenitor cell markers and differentiated cell markers with ZSCAN4 in each compartment of the pancreas may indicate the presence of facultative progenitors for both exocrine and endocrine cells in the adult pancreas.
Arene ruthenium(II) Complexes: The Promising Chemotherapeutic Agent in Inhibiting the Proliferation, Migration and Invasion (2015)

Arene ruthenium(II) complexes have been widely investigated as one of the most promising candidates in chemotherapy because of their low toxicity and high inhibiting activity against the proliferation, invasion, migration, and angiogenesis of various tumors in vitro and in vivo. This review highlights the recent developments in different chemical types of arene Ru(II) complexes, as well as their biological activity and underlying mechanisms. The sustained efforts in this aspect of arene Ru(II) complexes would be essential in developing novel anti-tumor agents in the near future.
High-Resolution Differential Ion Mobility Separations Using Helium-Rich Gases (2010)

Analyses of complex mixtures and characterization of ions increasingly involve gas-phase separations by ion mobility spectrometry (IMS) and particularly differential or field asymmetric waveform IMS (FAIMS) based on the difference of ion mobility in strong and weak electric fields. The key advantage of FAIMS is substantial orthogonality to mass spectrometry (MS), which makes FAIMS/MS hybrid a powerful analytical platform of broad utility. However, the potential of FAIMS has been constrained by limited resolution. Here, we report that the use of gas mixtures comprising up to 75% He dramatically increases the FAIMS separation capability, with the resolving power for peptides and peak capacity for protein digests reaching and exceeding 100. The resolution gains extend to small molecules, where previously unresolved isomers can now be separated. These performance levels open major new applications of FAIMS in proteomic and other biomolecular analyses.
Improving the Affinity and Selectivity of a Nonpeptide Series of Cholecystokinin-B/Gastrin Receptor Antagonists Based on the Dibenzobicyclo[2.2.2]octane Skeleton (1995)

We have recently described a novel series of nonpeptidic cholecystokinin-B (CCKB)/gastrin receptor antagonists based on a dibenzobicyclo[2.2.2]octane skeleton. We wish now to report on compounds arising out of our earlier work which have substantially greater affinity as antagonists for the CCKB/gastrin receptor system and which maintain, or improve on, the already high selectivity with respect to CCKA receptors. Thus, cis-7-[[[(1S)-[[3,5-dicarboxy-phenyl)amino]carbonyl]-2- phenylethyl]amino]carbonyl]-8-[[(1-adamantylmethyl)amino]- carbonyl]-2,3:5,6-dibenzobicyclo[2.2.2]octane expressed a pKi of 8.80 in mouse cortical membranes at CCKB/gastrin receptors. The selectivity for these receptors over CCKA receptors was in the order of 1000-fold.
Abasic Site-Containing DNAzyme and Aptamer for Label-Free Fluorescent Detection of Pb<sup>2+</sup> and Adenosine with High Sensitivity, Selectivity, and Tunable Dynamic Range (2009)

An abasic site called dSpacer has been introduced into duplex regions of the 8-17 DNAzyme and adenosine aptamer for label-free fluorescent detection of Pb(2+) and adenosine, respectively. The dSpacer can bind an extrinsic fluorescent compound, 2-amino-5,6,7-trimethyl-1,8-naphthyridine (ATMND), and quench its fluorescence. Addition of Pb(2+) enables the DNAzyme to cleave its substrate and release ATMND from DNA duplex, recovering the fluorescence of ATMND. Similarly, the presence of adenosine induces structural switching of the aptamer, resulting in the release of ATMND from the DNA duplex and a subsequent fluorescence enhancement. Under optimized conditions, this label-free method exhibits detection limits of 4 nM for Pb(2+) and 3.4 muM for adenosine, which are even lower than those of the corresponding labeled-DNAzyme and aptamer sensors. These low detection limits have been obtained without compromising any of the selectivity of the sensors. Finally, the dynamic range of the adenosine sensor has been tuned by varying the number of hybridized base-pairs in the aptamer duplex. The method demonstrated here can be applied for label-free detection and quantification of a broad range of analytes using other DNAzymes and aptamers.
Microbiota's Influence on Immunity (2013)

Our understanding of the complex innate immune response is increasing rapidly. Its role in the protection against viral or bacterial pathogens is essential for the survival of an organism. However, it is equally important to avoid unregulated inflammation because innate immune responses can cause or promote chronic autoinflammatory diseases such as gout, atherosclerosis, type 2 diabetes or certain aspects of the metabolic syndrome. In this book leading international experts in the field of innate immunity share their findings, define the 'state of the art' in this field and evaluate how insight into the molecular basis of these diseases could help in the design of new therapies. A tremendous amount of work on the innate immune response has been done over the last fifteen years, culminating in the 2011 Nobel Prize in Physiology/Medicine awarded for the discoveries of Toll genes in immunity in flies, membrane-bound Toll-like receptors in mammals, and dendritic cells as initiators of adaptive immunity.
SARS-CoV-2 D614G variant exhibits efficient replication ex vivo and transmission in vivo (2020)

The spike aspartic acid-614 to glycine (D614G) substitution is prevalent in global severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) strains, but its effects on viral pathogenesis and transmissibility remain unclear. We engineered a SARS-CoV-2 variant containing this substitution. The variant exhibits more efficient infection, replication, and competitive fitness in primary human airway epithelial cells but maintains similar morphology and in vitro neutralization properties, compared with the ancestral wild-type virus. Infection of human angiotensin-converting enzyme 2 (ACE2) transgenic mice and Syrian hamsters with both viruses resulted in similar viral titers in respiratory tissues and pulmonary disease. However, the D614G variant transmits significantly faster and displayed increased competitive fitness than the wild-type virus in hamsters. These data show that the D614G substitution enhances SARS-CoV-2 infectivity, competitive fitness, and transmission in primary human cells and animal models.
Automated white‐matter tractography using a probabilistic diffusion tensor atlas: Application to temporal lobe epilepsy (2008)

Diffusion-weighted magnetic resonance imaging allows researchers and clinicians to identify individual white matter fiber tracts and map their trajectories. The reliability and interpretability of fiber-tracking procedures is improved when a priori anatomical information is used as a guide. We have developed an automated method for labeling white matter fiber tracts in individual subjects based on a probabilistic atlas of fiber tract locations and orientations. The probabilistic fiber atlas contains 23 fiber tracts and was constructed by manually identifying fiber tracts in 21 healthy controls and 21 patients with temporal lobe epilepsy (TLE). The manual tract identification method required approximately 40 h of manual editing by a trained image analyst using multiple regions of interest to select or exclude streamline fibers. Identification of fiber tracts with the atlas does not require human intervention, but nonetheless benefits from the a priori anatomical information that was used to manually identify the tracts included in the atlas. We applied this method to compare fractional anisotropy--thought to be a measure of white matter integrity--in individual fiber tracts between control subjects and patients with TLE. We found that the atlas-based and manual fiber selection methods produced a similar pattern of results. However, the between-group effect sizes using the atlas-derived fibers were generally as large or larger than those obtained with manually selected fiber tracks.
Purification and Characterization of a Marine Bacterial  -Galactoside  2,6-Sialyltransferase from Photobacterium damsela JTO16O (1996)

A bacterial sialyltransferase, named sialyltransferase 0160, was purified from a marine bacterium that had been isolated from seawater from Sagami Bay, Kanagawa. This strain has been identified as Photobacterium damsela, and named P. damsela JT0160. Sialyltransferase 0160 was purified 688-fold to homogeneity from the crude extract of the cells with a yield of 19% using a combination of anion exchange chromatography, hydroxyapatite chromatography, gel filtration chromatography, and affinity chromatography. The purified enzyme migrated as a single band (61 kDa) on sodium dodecyl sulfate-polyacrylamide gel. This sialyltransferase was found to be a beta-galactoside alpha 2,6-sialyltransferase [EC 2.4.99.1] which catalyzes the incorporation of NeuAc from CMP-NeuAc into the galactose residue of the carbohydrate chain at position 6 on the basis of an analysis of the enzymatic reaction products with HPLC, 1H-, 13C-NMR spectroscopy, and fast atom bombardment mass spectroscopy.
Super non-linear RRAM with ultra-low power for 3D vertical nano-crossbar arrays (2016)

Vertical crossbar arrays provide a cost-effective approach for high density three-dimensional (3D) integration of resistive random access memory. However, an individual selector device is not allowed to be integrated with the memory cell separately. The development of V-RRAM has impeded the lack of satisfactory self-selective cells. In this study, we have developed a high performance bilayer self-selective device using HfO2 as the memory switching layer and a mixed ionic and electron conductor as the selective layer. The device exhibits high non-linearity (>10(3)) and ultra-low half-select leakage (<0.1 pA). A four layer vertical crossbar array was successfully demonstrated based on the developed self-selective device. High uniformity, ultra-low leakage, sub-nA operation, self-compliance, and excellent read/write disturbance immunity were achieved. The robust array level performance shows attractive potential for low power and high density 3D data storage applications.
A double recombinant herpes virus of turkeys for the protection of chickens against Newcastle, infectious laryngotracheitis and Marek’s diseases (2018)

A double recombinant strain of herpes virus of turkeys (HVT) was constructed that contains the fusion (F) gene from Newcastle disease virus (NDV) and the gD plus gI genes from infectious laryngotracheitis virus (ILTV) inserted into a non-essential region of the HVT genome. Expression of the F protein was controlled by a human cytomegalovirus promoter, whereas expression of gD plus gI was driven by an ILTV promoter. The double recombinant vaccine virus (HVT-NDV-ILT) was fully stable genetically and phenotypically following extended passage in cell culture and infection of chickens. Safety of the vaccine virus was confirmed by overdose and backpassage studies in specific-pathogen-free chickens. Chickens vaccinated with a single dose of HVT-NDV-ILT administered by the in ovo route were highly protected from challenge with the velogenic NDV (GB Texas), ILTV (LT 96-3) and Marek's disease virus (GA 5) strains (97%, 94% and 97%, respectively). Similarly, chickens vaccinated with a single dose by subcutaneous (SC) route at 1 day of age were highly protected from challenge with the same three viruses (100%, 100%, and 88%, respectively). The protection level of a single dose given by in ovo or SC route against challenge with a virulent Marek's disease virus strain demonstrates that insertion of multiple genes from two different pathogens within the HVT genome had no adverse effect on the capacity of HVT to protect against Marek's disease. These results demonstrate that HVT-NDV-ILT is a safe and efficacious vaccine for simultaneous control of NDV, ILTV and Marek's diseases.
Fault-tolerant distributed simulation (1998)

In traditional distributed simulation schemes, the entire simulation needs to be restarted if any of the participating logical processes (LPs) crash. This is highly undesirable for long running simulations. Some form of fault tolerance is required to minimize the wasted computation. A rollback based optimistic fault tolerance scheme is integrated with an optimistic distributed simulation scheme. In rollback recovery schemes, checkpoints are periodically saved on stable storage. After a crash, these saved checkpoints are used to restart the computation. We make use of the novel insight that a failure can be modeled as a straggler event with the receive time equal to the virtual time of the last checkpoint saved on stable storage. This results in saving of implementation efforts, as well as reduced overheads. We define stable global virtual time (SGVT), as the virtual time such that no state with a lower timestamp will ever be rolled back despite crash failures. A simple change is made in existing GVT algorithms to compute SGVT. Our use of transitive dependency tracking eliminates antimessages. LPs are clubbed in clusters to minimize stable storage access time.