gebhart/patrepeval huggingface dataset:
Tuberculin Skin Testing Compared with T-Cell Responses to <i>Mycobacterium tuberculosis</i> -Specific and Nonspecific Antigens for Detection of Latent Infection in Persons with Recent Tuberculosis Contact (2001)

The tuberculin skin test (TST) is used for the identification of latent tuberculosis (TB) infection (LTBI) but lacks specificity in Mycobacterium bovis BCG-vaccinated individuals, who constitute an increasing proportion of TB patients and their contacts from regions where TB is endemic. In previous studies, T-cell responses to ESAT-6 and CFP-10, M. tuberculosis-specific antigens that are absent from BCG, were sensitive and specific for detection of active TB. We studied 44 close contacts of a patient with smear-positive pulmonary TB and compared the standard screening procedure for LTBI by TST or chest radiographs with T-cell responses to M. tuberculosis-specific and nonspecific antigens. Peripheral blood mononuclear cells were cocultured with ESAT-6, CFP-10, TB10.4 (each as recombinant antigen and as a mixture of overlapping synthetic peptides), M. tuberculosis sonicate, purified protein derivative (PPD), and short-term culture filtrate, using gamma interferon production as the response measure. LTBI screening was by TST in 36 participants and by chest radiographs in 8 persons. Nineteen contacts were categorized as TST negative, 12 were categorized as TST positive, and 5 had indeterminate TST results. Recombinant antigens and peptide mixtures gave similar results. Responses to TB10.4 were neither sensitive nor specific for LTBI. T-cell responses to ESAT-6 and CFP-10 were less sensitive for detection of LTBI than those to PPD (67 versus 100%) but considerably more specific (100 versus 72%). The specificity of the TST or in vitro responses to PPD will be even less when the proportion of BCG-vaccinated persons among TB contacts evaluated for LTBI increases.
Self-powered sensing and time-stamping of rare events using CMOS Fowler-Nordheim tunneling timers (2016)

This paper explores the use of continuous-time Fowler-Nordheim (FN) tunneling for implementing self-powered CMOS sensors that can be used to simultaneously measure and time-stamp occurrences of rare signal events. At the core of the proposed design is a floating-gate device where the FN based electron tunneling is induced through the thin gate-oxide and the control gate is used to couple the signal that is being measured and time-stamped. The signal then modulates the shape of the FN tunneling barrier which is captured by the tunneling rate of electrons being stored on the floating-gate. Thus the sensor continuously operates without the need for any external powering and the data from the sensor can be retrieved to reconstruct the occurrence of events offline. The proof-of-concept has been validated using prototypes fabricated in a standard 0.5-μm CMOS process and the measurement results show that less than 100 fJ of sensing energy is required for event recording and time-stamping.
Wavelength dependence of light propagation in long fibre links (1978)

A model for the analysis of the bandwidth along an optical fibre route has been extended to include the variations of the bandwidth with wavelength. It is concluded that alternately jointing under and over compensated fibres has the advantage not only of producing high bandwidth but also stabilizing it with wavelength.
Clustering algorithms for wireless ad hoc networks (2000)

Efficient clustering algorithms play a very important role in the fast connection establishment of ad hoc networks. In this paper, we describe a communication model that is derived directly from that of Bluetooth, an emerging technology for pervasive computing; this technology is expected to play a major role in future personal area network applications. We further propose two new distributed algorithms for clustering in wireless ad hoc networks. The existing algorithms often become infeasible because they use models where the discovering devices broadcast their Ids and exchange substantial information in the initial stages of the algorithm.
Enhancement of Viewing Performance of New Transflective In-Plane Switching Liquid Crystal Displays Using In-Cell Retarder(s) (2008)

High-viewing-performance transflective in-plane switching (IPS) liquid crystal displays (LCDs) with in-cell retarders have been developed. Transflective LCDs have pixels with transmissive and reflective display portions; the transmissive display portion is designed to have the same structure as the transmissive IPS-LCDs, and the reflective display portion is formed by patterning the in-cell retarder. The latter is designed to give a normally close mode characteristic, which has a brightness–voltage (B–V) curve similar to that of the former. The reflective display portions, which work as quarter-wave plates at dark states, can be classified into five structures according to the number of in-cell retarder(s) and the stacking order of the liquid crystal layer. In this study, on the basis of the five possible structures, the viewing performances of the transflective IPS-LCDs are investigated by simulation, and suitable solutions are also discussed.
Predicting a local recurrence after breast-conserving therapy by gene expression profiling (2006)

Our findings indicate that gene expression profiling can identify subgroups of patients at increased risk of developing a local recurrence after breast-conserving therapy.
Bronchodilator and antiulcer phenoxypyrimidinones (1980)

Series of 5-phenoxy-2(1H)-pyrimidinones, 5-phenoxy-4(3H)-pyrimidinones, and related compounds were prepared in a follow-up of a lead prepared as a potential cyclic nucleotide regulating agent. Compounds were evaluated for bronchodilator activity in histamine-challenged guinea pigs and for anticulcer activity in a cold-restraint, stressed rat ulcer model. Bronchodilator activity comparable to, or greater than, that of theophylline was found in both the 2(1H)- and 4(3H)-pyrimidinone series and was most prominent in analogues containing either an electron-withdrawing or -donating substituent in the para position of the phenoxy ring. Significant antiulcer activity was observed only in the 2(1H)-pyrimidinone series among three closely related analogues. One of these, 5-(m-methylphenoxy)-2(1H)-pyrimidinone (3), exhibited more potent antiulcer effects than the clinically useful antiulcer agent carbenoxolone, without demonstrating bronchodilator activity.
Autonomous reconstruction of unknown indoor scenes guided by time-varying tensor fields (2017)

Autonomous reconstruction of unknown scenes by a mobile robot inherently poses the question of balancing between exploration efficacy and reconstruction quality. We present a navigation-by-reconstruction approach to address this question, where moving paths of the robot are planned to account for both global efficiency for fast exploration and local smoothness to obtain high-quality scans. An RGB-D camera, attached to the robot arm, is dictated by the desired reconstruction quality as well as the movement of the robot itself. Our key idea is to harness a time-varying tensor field to guide robot movement, and then solve for 3D camera control under the constraint of the 2D robot moving path. The tensor field is updated in real time, conforming to the progressively reconstructed scene. We show that tensor fields are well suited for guiding autonomous scanning for two reasons: first, they contain sparse and controllable singularities that allow generating a locally smooth robot path, and second, their topological structure can be used for globally efficient path routing within a partially reconstructed scene. We have conducted numerous tests with a mobile robot, and demonstrate that our method leads to a smooth exploration and high-quality reconstruction of unknown indoor scenes.
Right Atrial Septal Electrode for Reducing the Atrial Defibrillation Threshold (2001)

The ADFT of the standard RAA-->CS configuration may be markedly reduced with an additional electrode situated at the RSP.
Endo‐Porter: A Novel Reagent for Safe, Effective Delivery of Substances into Cells (2005)

Delivering large molecules into the cytosol of animal cells without damaging the cells has been one of the toughest challenges in biology. Endo-Porter is a weak-base amphiphilic peptide that was designed to deliver morpholino antisense oligomers and other non-ionic substances into the cytosol/nuclear compartment of cells by an endocytosis-mediated process that avoids damaging the plasma membrane of the cell. This prevents the loss of vital cell contents and the attendant high cell toxicity typical of most delivery systems. To deliver a substance into cells simply add that substance to the medium covering the cells, followed by addition of the pre-formulated Endo-Porter solution and swirl to mix. While most delivery reagents begin to show serious toxicity after just a few hours and are relatively ineffective in the presence of serum, delivery with Endo-Porter may be continued for 24 hours, or even 48 hours, without undue toxicity, and Endo-Porter is particularly effective in the presence of the 10% serum commonly used with cultured cells. The mechanism of delivery, entails rapid adsorption of Endo-Porter to cell surfaces, but this adsorption to the cell surface does not damage the plasma membrane. The membrane-bound Endo-Porter is rapidly endocytosed, along with any substances present in the medium (ie., the cargo one wishes to deliver). Upon subsequent acidification of the endosome (a natural process) the Endo-Porter contained within that endosome is converted to its poly-cationic form, which acts to permeabilize the endosomal membrane. This acid-induced permeabilization of the endosomal membrane allows any co-endocytosed cargo to pass from the endosome into the cytosol of the cell. This paper describes the basic design strategy used to develop Endo-Porter, test systems used to guide that development, and the effects of various structural parameters, including size and composition of the lipophilic face, size and composition of the weak-base face, and the relationship between peptide length and delivery efficiency in the presence of serum.
Optimal wiring topology for electromigration avoidance considering multiple layers and obstacles (2010)

Due to excessive current densities, electromigration may trigger a permanent open- or short-circuit failure in signal wires or power networks in analog or mixed-signal circuits. As the feature size keeps shrinking, this effect becomes a key reliability concern. Hence, in this paper, we focus on wiring topology generation for avoiding electromigration at the routing stage. Prior works tended towards heuristics; on the contrary, we first claim this problem belongs to class P instead of class NP-hard. Our breakthrough is, via the proof of the greedy-choice property, we successfully model this problem on a multi-source multi-sink flow network and then solve it by a strongly polynomial time algorithm. Experimental results prove the effectiveness and efficiency of our algorithm.
Optoacoustic monitoring of total hemoglobin concentration: In vitro and in vivo studies (2004)

We performed in vitro and in vivo tests of optoacoustic monitoring of total hemoglobin concentration. The results of the in vitro measurements in blood and pilot in vivo studies in volunteers suggest that the optoacoustic technique may be used for noninvasive and continuous monitoring of total hemoglobin concentration.
Norovirus Protease Structure and Antivirals Development (2021)

Human norovirus (HuNoV) infection is a global health and economic burden. Currently, there are no licensed HuNoV vaccines or antiviral drugs available. The protease encoded by the HuNoV genome plays a critical role in virus replication by cleaving the polyprotein and is an excellent target for developing small-molecule inhibitors. The current strategy for developing HuNoV protease inhibitors is by targeting the enzyme's active site and designing inhibitors that bind to the substrate-binding pockets located near the active site. However, subtle differential conformational flexibility in response to the different substrates in the polyprotein and structural differences in the active site and substrate-binding pockets across different genogroups, hamper the development of effective broad-spectrum inhibitors. A comparative analysis of the available HuNoV protease structures may provide valuable insight for identifying novel strategies for the design and development of such inhibitors. The goal of this review is to provide such analysis together with an overview of the current status of the design and development of HuNoV protease inhibitors.
X-Linked Alport Dogs Demonstrate Mesangial Filopodial Invasion of the Capillary Tuft as an Early Event in Glomerular Damage (2016)

In a large animal model, the induction of mesangial filopodial invasion of the glomerular capillary loop leading to the irregular deposition of laminin 211 is an early initiating event in Alport glomerular pathology.
Insecticidal, Miticidal, and Ovicidal Activity of 5,6-Dihydro-4H-1,3,4-Oxadiazines (1993)

Journal Article Insecticidal, Miticidal, and Ovicidal Activity of 5,6-Dihydro-4H-1,3,4-Oxadiazines Get access Mark A. Dekeyser, Mark A. Dekeyser Search for other works by this author on: Oxford Academic PubMed Google Scholar Paul T. McDonald, Paul T. McDonald Search for other works by this author on: Oxford Academic PubMed Google Scholar Gilbert W. Angle, Jr., Gilbert W. Angle, Jr. Search for other works by this author on: Oxford Academic PubMed Google Scholar David M. Borth, David M. Borth Search for other works by this author on: Oxford Academic PubMed Google Scholar Roger G. H. Downer Roger G. H. Downer Search for other works by this author on: Oxford Academic PubMed Google Scholar Journal of Economic Entomology, Volume 86, Issue 5, 1 October 1993, Pages 1339–1343, https://doi.org/10.1093/jee/86.5.1339 Published: 01 October 1993 Article history Received: 14 September 1992 Accepted: 23 April 1993 Published: 01 October 1993
The immune system in ultraviolet carcinogenesis. (1996)

The immune system plays an important role in ultraviolet (UV) carcinogenesis by contributing to host resistance against skin cancer development. UV radiation, however, circumvents immune surveillance against skin cancers by modulating the immune response in a way that favors tumor development. Studies addressing the cellular mechanisms by which UV radiation modifies immune function are summarized. These studies demonstrate that UV irradiation of the skin produces both local, nonspecific immune suppression that inhibits immune effector functions within irradiated skin, as well as systemic, specific immune suppression against antigens introduced at a critical time after exposure to UV radiation. Evidence is presented suggesting that the production of cytokines by epidermal cells in response to UV-induced DNA damage is an important component of UV-induced specific immune suppression.
Corneal Endothelial Expansion Promoted by Human Bone Marrow Mesenchymal Stem Cell-Derived Conditioned Medium (2013)

Healthy corneal endothelium is essential for maintaining corneal clarity, as the damage of corneal endothelial cells and loss of cell count causes severe visual impairment. Corneal transplantation is currently the only therapy for severe corneal disorders. The greatly limited proliferative ability of human corneal endothelial cells (HCECs), even in vitro, has challenged researchers to establish efficient techniques for the cultivating HCECs, a pivotal issue for clinical applications. The aim of this study was to evaluate conditioned medium (CM) obtained from human bone marrow-derived mesenchymal stem cells (MSCs) (MSC-CM) for use as a consistent expansion protocol of HCECs. When HCECs were maintained in the presence of MSC-CM, cell morphology assumed a hexagonal shape similar to corneal endothelial cells in vivo, as opposed to the irregular cell shape observed in control cultures in the absence of MSC-CM. They also maintained the functional protein phenotypes; ZO-1 and Na(+)/K(+)-ATPase were localized at the intercellular adherent junctions and pump proteins of corneal endothelium were accordingly expressed. In comparison to the proliferative potential observed in the control cultures, HCECs maintained in MSC-CM were found to have more than twice as many Ki67-positive cells and a greatly increased incorporation of BrdU into DNA. MSC-CM further facilitated the cell migration of HCECs. Lastly, the mechanism of cell proliferation mediated by MSC-CM was investigated, and phosphorylation of Akt and ERK1/2 was observed in HCECs after exposure to MSC-CM. The inhibitor to PI 3-kinase maintained the level of p27(Kip1) for up to 24 hours and greatly blocked the expression of cyclin D1 and D3 during the early G1 phase, leading to the reduction of cell density. These findings indicate that MSC-CM not only stimulates the proliferation of HCECs by regulating the G1 proteins of the cell cycle but also maintains the characteristic differentiated phenotypes necessary for the endothelial functions.
THE HERSHEY HALO JIG (1981)

The Hershey Halo Jig is a device for simplifying the application of the cranial halo ring. Secured by the patient's own body weight, the jig safely supports both the patient's head and the halo ring, leaving the surgeon's hands free for skull pin insertion. The head support is adjustable allowing cervical spine flexion or extension according to stability requirements. The halo jig significantly increases the speed, accuracy, and safety of halo application.
Inhibition of <i>Clostridium perfringens</i> by a Novel Strain of <i>Bacillus subtilis</i> Isolated from the Gastrointestinal Tracts of Healthy Chickens (2005)

The objectives of this study were to isolate beneficial strains of microorganisms from the gastrointestinal tracts of healthy chickens and to screen them against Clostridium perfringens, a causative agent of necrotic enteritis in poultry. One of the bacteria isolated, a strain of Bacillus subtilis, was found to possess an anticlostridial factor that could inhibit the C. perfringens ATCC 13124 used in this study. The anticlostridial factor produced by B. subtilis PB6 was found to be fully or partially inactivated in the presence of pronase, trypsin, and pepsin. In contrast, the antimicrobial activity of the anticlostridial factor was not affected by treatment at 100 or 121 degrees C or by treatment with any of the organic solvents used in the study. The optimum growth temperature and optimum pH for production of the anticlostridial factor were 37 degrees C and 6.20, respectively. Using the mass spectroscopy-mass spectroscopy technique, the apparent molecular mass of the anticlostridial factor was estimated to be in the range from 960 to 983 Da. In terms of the antimicrobial spectrum, the anticlostridial factor was inhibitory toward various strains of C. perfringens implicated in necrotic enteritis in poultry, Clostridium difficile, Streptococcus pneumoniae, Campylobacter jejuni, and Campylobacter coli.
Lagos bat virus virulence in mice inoculated by the peripheral route (2009)

Lagos bat virus (LBV) constitutes genotype (gt) 2 in the Lyssavirus genus. In contrast to the gt1 lyssavirus, rabies virus (RABV), LBV was reported to have markedly reduced levels of peripheral pathogenicity. However, this opinion was based on a study of one isolate of LBV only and the reduction in pathogenicity was essentially attributed to the amino-acid substitution at position 333 of glycoprotein ectodomain. In the present study we have demonstrated that peripheral pathogenicity of representatives of LBV in a murine model is as high as that of RABV. Comparison of amino-acid substitutions among the viral glycoproteins, demonstrated significant differences within two antigenic sites between different phylogenetic lineages of LBV. Such molecular variability potentially contributes to differences in peripheral pathogenicity of lyssaviruses.
Progress toward a low-cost millimeter-wave silicon radio (2006)

This paper discusses the circuits, packaging, and antennas needed to realize a low-cost millimeter-wave transceiver with integrated antenna in silicon technology. The principal application envisioned is in high-speed 60-GHz wireless networks, but the concepts may be applicable to other products as well, such as 77-GHz radar sensors. Circuit results are presented for both 60 and 77 GHz, including an LNA, a highly-integrated direct downconverter, a mixer for a superheterodyne receiver, and a power amplifier. Packaging issues which arise at millimeter-wave frequencies are discussed, and a packaging approach involving a Si IC and a planar antenna in the same package is described. Measurement results for a planar Vivaldi antenna are presented as an example.
PHYTOCHROME‐REGULATED EXPRESSION OF GENES ENCODING LIGHT‐HARVESTING CHLOROPHYLL a/b‐PROTEIN IN TWO LONG HYPOCOTYL MUTANTS and WILD TYPE PLANTS OF Arabidopsis thaliana* (1990)

The cab genes which encode the light-harvesting chlorophyll a/b-protein (LHCP) are expressed normally with respect to phytochrome regulation in the hy-3 and hy-5 long hypocotyl mutants of Arabidopsis thaliana. In etiolated seedlings of these mutants as well as of the wild type, 1 min of red light elevates cab mRNA levels substantially within 2 h; this increase is reversed if far-red light is given immediately after the red light treatment. We conclude that the genetic defects in these mutants do not affect steps in the signal transduction pathway leading to the regulated expression of cab genes. Additionally, the mRNA from one of the three known A. thaliana cab genes, AB140, is similar in quantity to the mRNAs from the other two, AB165 and AB180, in dark-grown seedlings of hy-3 and hy-5 as well as the parent A. thaliana (Landsberg) after a brief red light treatment. This aspect of cab gene expression differs from the strain Columbia of A. thaliana in which AB140 mRNA is the predominant message. In mature white light-grown plants of the strain Columbia, AB140 as well as a combination of AB165 and AB180 mRNAs are expressed at high levels, suggesting that AB165 and/or AB180 may be developmentally regulated.
A nonconjugated radical polymer glass with high electrical conductivity (2018)

Solid-state conducting polymers usually have highly conjugated macromolecular backbones and require intentional doping in order to achieve high electrical conductivities. Conversely, single-component, charge-neutral macromolecules could be synthetically simpler and have improved processibility and ambient stability. We show that poly(4-glycidyloxy-2,2,6,6-tetramethylpiperidine-1-oxyl), a nonconjugated radical polymer with a subambient glass transition temperature, underwent rapid solid-state charge transfer reactions and had an electrical conductivity of up to 28 siemens per meter over channel lengths up to 0.6 micrometers. The charge transport through the radical polymer film was enabled with thermal annealing at 80°C, which allowed for the formation of a percolating network of open-shell sites in electronic communication with one another. The electrical conductivity was not enhanced by intentional doping, and thin films of this material showed high optical transparency.