gebhart/patrepeval huggingface dataset:
Salivary receptors for recombinant fimbrillin of Porphyromonas gingivalis (1994)

Fimbriae are considered important in the adherence and colonization of Porphyromonas gingivalis in the oral cavity. It has been demonstrated that purified fimbriae bind to whole human saliva adsorbed to hydroxyapatite (HAP) beads, and the binding appears to be mediated by specific protein-protein interactions. Recently, we expressed the recombinant fimbrillin protein (r-Fim) of P. gingivalis corresponding to amino acid residues 10 to 337 of the native fimbrillin (A. Sharma, H.T. Sojar, J.-Y. Lee, and R.J. Genco, Infect. Immun. 61:3570-3573, 1993). We examined the ability of individual salivary components to promote the direct attachment of r-Fim to HAP beads. Purified r-Fim was radiolabeled with 125I and incubated with HAP beads which were coated with saliva or purified individual salivary components. Whole, parotid, and submandibular-sublingual salivas increased the binding of 125I-r-Fim to HAP beads. Submandibular-sublingual saliva was most effective in increasing the binding of 125I-r-Fim to HAP beads (1.8 times greater than that to uncoated HAP beads). The binding of 125I-r-Fim to HAP beads coated with acidic proline-rich protein 1 (PRP1) or statherin was four and two times greater, respectively, than that to uncoated HAP beads. PRP1 and statherin molecules were also found to bind 125I-r-Fim in an overlay assay. The binding of intact P. gingivalis cells to HAP beads coated with PRP1 or statherin was also enhanced, by 5.4 and 4.3 times, respectively, over that to uncoated HAP beads. The interactions of PRP1 and statherin with 125I-r-Fim were not inhibited by the addition of carbohydrates or amino acids. PRP1 and statherin in solution did not show inhibitory activity on 125I-r-Fim binding to HAP beads coated with PRP1 or statherin. These results suggest that P. gingivalis fimbriae bind strongly through protein-protein interactions to acidic proline-rich protein and statherin molecules which coat surfaces.
Micromachined piezoelectric spirals and ultra-compliant packaging for blood pressure energy harvesters powering medical implants (2013)

This paper introduces a novel energy harvesting technique and an associated packaged device aimed at powering intracardiac active medical devices by scavenging energy from ordinary blood pressure variations. We present the innovative concept of a micro-bellows packaged implant that deforms due to cyclic blood pressure variations in the cardiac cavities. This bellows transmits stresses on a spiral-shaped piezoelectric transducer that converts mechanical deformations into electric energy. The particular shapes of the different components (bellows and spiral) are designed to dramatically increase the mechanical energy that is harvested. The miniature bellows design, fabrication through electrodeposition for high compliance and hermeticity, and experimental characterization are presented. The specific challenges that arise in spiral shapes piezoelectric harvesters working in bending mode are discussed and we present a method to predict the non-trivial optimal electrode placements for maximum efficiency. This design process is completed by an associated microfabrication method and three types of piezoelectric bimorph spiral samples with doubled-sided microstructured electrode patterns are presented. The experimental performances of these prototypes are confronted to numerical simulation. A power of 4.15 μW/cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sup> at 1.5 Hz has been obtained for the best design and we predict that tens of micro-Watts per cubic centimeter could be obtained using similar design process and material.
Cryogen Spray Cooling during Nd:YAG Laser Treatment of Hemangiomas A Preliminary Animal Model Study (1997)

The results demonstrate that CSC is effective in protecting the epidermis and papillary dermis, while achieving deep tissue photocoagulation during Nd:YAG laser irradiation. Further pilot studies in humans appear warranted.
Remote and dynamic assessment of reliability in electromechanical actuator systems (2018)

This paper describes a method for assessing dynamic reliability of electromechanical actuator systems using limited external sensor measurements, with the overall goal of providing optimized maintenance scheduling for a fleet of deployed systems. To account for special cause failures in the actuators, nominal reliability is adjusted using an accumulated special hazard function. The paper illustrates a method for obtaining statistical control thresholds using Monte Carlo analysis coupled with a detailed electromechanical model describing the actuator system. Numerical simulation studies demonstrate the approach.
A new method for measuring properties of liquid by using a single quartz crystal microbalance (2017)

As an ultra-sensitive mass sensing device, quartz crystal microbalance (QCM) can also be used to detect a variety of analytes in liquid environment. Previous works reported separate density and viscosity measurements of liquid by using dual QCMs. In this work, we present a novel theoretical model and a new method to separate density and viscosity measurements of liquid only using a single QCM, based on the frequency response analysis. Experimental results demonstrate the merit of the single QCM setup in determining the physical properties of liquid is an exciting new solvent.
Abstract 12649: A Faster and Safe Way to Initiate Sotalol Therapy for Patients With Atrial Fibrillation or Flutter (2020)

Sotalol drug therapy is frequently employed to prevent the recurrence of highly symptomatic atrial fibrillation or flutter (AF, AFb). Sotalol can cause QTc prolongation that is proportional to blood concentration. Excessive concentration can cause Torsade de Pointes ventricular tachycardia (TdP). Because the risk of TdP, initiation of oral sotalol therapy is mandated by FDA to be in-hospital for a minimum of three days under ECG monitoring with facilities and personnel able to provide cardiac resuscitation. With oral administration (bid), 3 days are needed to reach maximal steady-state blood concentrations (Cmax ss) and thus maximal QTc prolongation. Three hospital days in a telemetry bed is costly and considerable expenditure of time and resources. Availability of IV sotalol makes it possible to reduce the loading time from 3 days to 1 day. The IV to oral loading regimen has been developed by model informed drug development. Serum sotalol concentrations and corresponding QTc were obtained from a previously performed bioequivalence study in healthy volunteers who received a single dose of oral and IV sotalol. NONMEM software package was used for population pharmacokinetic modeling and simulation. First Order Conditional Estimation method with Interaction (FOCE-INTER) was used for computation. We chose to administer IV sotalol over 1 hour to obtain Cmax ss target in a timeframe convenient for medical staff to supervise. The simulation for loading to the Cmax target produced by 80 mg PO bid is shown in the Figure; 60 mg IV sotalol is administered over 1 hour followed by 80 mg oral sotalol at 5 hours from start of infusion end then a second oral dose of 80 mg at 12 hours. One can target 120 mg maintenance dose by loading 90 mg IV over 1 hr followed by 120 mg oral dosing. Sotalol concentration will peak in 2-4 hours following each oral administration, thus in 21 hour there will be 3 sotalol peak concentrations. This permits evaluation of QTc response and risk of TdP all within a 1 day admission.
A diphenylethynyl-terminated imide oligomer and its cured polymer (1995)

A novel amine end-capper containing two phenylethynyl groups was used to terminate an imide oligomer based on 4,4'-oxydiphthalic anhydride and 3,4'-oxydianiline. The properties of this cured di(phenylethynyl)-terminated imide oligomer (DPETI) were compared with the corresponding cured phenylethynyl-terminated imide oligomrner (DPETI). The initial results indicate that mechanical properties of these two systems are very similar. The cured DPETI displays a slightly higher T g and better solvent resistance in methyl ethyl ketone than the cured PETI-1.
HEVC deblocking filtering and decisions (2012)

The emerging High Efficiency Video Coding (HEVC) standard uses a block-based coding scheme, which may cause blocking artifacts, especially at lower bitrates. An adaptive in-loop deblocking filter is used in the standard to reduce visible artifacts at block boundaries. The deblocking filter detects artifacts at the block boundaries and attenuates them by applying a selected filter. This paper will present deblocking decisions and filtering operations that are used in HEVC.
Parasitics nonlinearity cancellation technique for split DAC architecture by using capacitive charge-pump (2010)

A voltage feedback charge-cancellation technique is proposed which prevents the conversion nonlinearity due to the parasitic effect of split DAC architecture in Successive Approximation Register (SAR) ADCs. A voltage feedback network operating as a capacitive charge-pump can efficiently detect and compensate the voltage error in each bit cycling, thus the conversion accuracy can be significantly improved. A 10b 80MS/s SAR ADC was demonstrated in 65nm CMOS technology. Simulation results show that the proposed charge-cancellation technique can improve the Effective Number of Bits (ENOB) from 8.5b to 9.6b and decrease the maximum DNL and INL from 3LSB to 0.5LSB and 1.65LSB to 0.74LSB, respectively, with only 100 μW power dissipation.
Control of unknown systems via deconvolution (1987)

The controller is a logical network which, when interfaced with a mechanical system or an electromechanical system, will cause the output variable of the system to closely follow any command signal received. It comprises shift registers, memory banks, accumulators, arithmetic units and preferably no software. Universal Controller keeps the error between the output of the model and the output of the control system small, regardless of the transfer function of the controlled system by adjusting a number of gains in its internal feedback loop in accordance with that error. It therefore can be applied to an arbitrary electromechanical system without prior knowledge of its transfer function.
Tight real-time synchronization of a microwave clock to an optical clock across a turbulent air path (2016)

The ability to distribute the precise time and frequency from an optical clock to remote platforms could enable future precise navigation and sensing systems. Here we demonstrate tight, real-time synchronization of a remote microwave clock to a master optical clock over a turbulent 4-km open air path via optical two-way time-frequency transfer. Once synchronized, the 10-GHz frequency signals generated at each site agree to 10<sup>-14</sup> at one second and below 10<sup>-17</sup> at 1000 seconds. In addition, the two clock times are synchronized to ±13 fs over an 8-hour period. The ability to phase-synchronize 10-GHz signals across platforms supports future distributed coherent sensing, while the ability to time-synchronize multiple microwave-based clocks to a high-performance master optical clock supports future precision navigation/timing systems.
BLV-CoCoMo-qPCR: comparison of other detection methods for BLV infection and kinetics analysis in experimental transmission of BLV in cattle (2011)

Bovine leukemia virus (BLV) infects cattle worldwide, imposing a severe economic impact on the dairy cattle industry. BLV is the etiological agent of enzootic bovine leukosis. Recently we developed a new quantitative real-time PCR method using Coordination of Common Motifs (CoCoMo) primers to measure the proviral load of known and novel BLV variants in clinical animals [1]. In this study, we analyzed a kinetic of the provirus and relevance of the BLV antibody titer.

First, we experimentally compared the sensitivity of our methods with previously reported for BLV provirus detection real-time PCR system, and determined the high sensitivity of our developed BLV-CoCoMo-qPCR.

Next, we estimated the sensitivities of the antibody detection methods such as ELISA, PHA and AGID and the provirus load estimated by BLV-CoCoMo-qPCR, using a total of 391 cattle. In three methods, high false-negative rate were observed at the range (100 copies/105cells) of low provirus copy number cattle. Meanwhile, a number of cattle with high antibody titer cannot be detected provirus. To investigate the reasons for the results, two cattle were experimentally infected with BLV and followed-up the titer of serum antibody and proviral load. We detected that proviral load were suppressed at the high antibody titer stage and it increased at the low antibody titer stage.

In this study, we clearly detected the inverse proportion between antibody titer and proviral load for the first time in natural host.

It suggested that quantification of proviral load is very important to halt the spread of BLV.
A compressed domain scheme for classifying block edge patterns (2005)

Detecting and classifying edge components in images find a wide range of applications from perceptually improved coding schemes to content-based searches. In this paper, a fast and systematic scheme is proposed to classify the edge orientation of each block in discrete cosine transform (DCT)-compressed images. Derived from a simple, but acceptable, pixel-domain algorithm, the proposed scheme performs directly on DCT coefficient domain and, thus, saves the arithmetic operations by a considerable amount. The performance of the proposed method is validated by the experiments against various natural images.
EFFECTS OF A MODIFIED VITRECTOMY PROBE IN SMALL-GAUGE VITRECTOMY (2016)

The modified probe could be useful in vitreoretinal surgery. It allows for faster vitreous removal while minimizing tractional forces on the retina. Moreover, any available probe can be modified by creating a hole in the inner duct.
A holographic sensor based on a rationally designed synthetic polymer (1998)

A new silver halide-containing holographic recording material has been designed and developed specifically for holographic chemical sensors. The hologram enables very small volume changes to be measured in a polymer layer throughout which the hologram is located. The holographic film is based on a fine-grain silver bromide emulsion suspended in a poly(vinyl alcohol) matrix crosslinked with Cr(III) ions. Cross-linking gives the material sufficient spatial integrity to allow a holographic image to be recorded, while maintaining adequate porosity and elasticity of the polymer matrix for sensing applications. The new material has been characterized with respect to its response to pH and compared with a traditional gelatin holographic film. The response to some ions and small molecules typically found in analytical samples has also been measured. Functional groups introduced covalently into the poly(vinyl alcohol) matrix transform the base matrix into a pH-responsive polymer with predictable swelling properties and which can be further derivatized to incorporate specific ligands. A rationally designed holographic sensor for trypsin has been developed from chemically synthesized artificial polymers. A trypsin substrate, the poly(amino acid) poly(L-lysine), was incorporated into poly(vinyl alcohol) holograms to create a 'designed' holographic material which was degraded in a concentration-dependent manner by trypsin. Extensions of this approach to other hydrolytic enzymes are briefly discussed.
Cost Evaluation of The Improvement of Twisted Ate Pairing That Uses Integer Variable X of Small Hamming Weight (2009)

Barreto–Naehrig (BN) curve has been introduced as an efficient pairing-friendly elliptic curve over prime field F(p) whose embedding degree is 12. The characteristic and Frobenius trace are given as polynomials of integer variable X. The authors proposed an improvement of Miller's algorithm of twisted Ate pairing with BN curve by applying X of small hamming weight in ITC–CSCC2008; however, its cost evaluation has not been explicitly shown. This paper shows the detail of the cost evaluation.
Human Cytotoxic CD4+ T Cells Recognize HLA-DR1-Restricted Epitopes on Vaccinia Virus Proteins A24R and D1R Conserved among Poxviruses (2007)

We previously demonstrated that vaccinia virus (VV)-specific CD4(+) cytolytic T cells can persist for >50 years after immunization against smallpox in the absence of re-exposure to VV. Nevertheless, there have been few studies focusing on CD4(+) T cell responses to smallpox vaccination. To ensure successful vaccination, a candidate vaccine should contain immunodominant CD4(+) T cell epitopes as well as CD8(+) T and B cell epitopes. In the present study, we established cytotoxic CD4(+) T cell lines from VV-immune donors, which recognize epitopes in VV proteins D1R and A24R in association with HLA-DR1 Ags. Comparisons of sequences between different members of the poxvirus family show that both epitopes are completely conserved among VV, variola viruses, and most mammalian poxviruses, including monkeypox, cowpox, and ectromelia. The CD4(+) T cell lines lysed VV-infected, Ag- and peptide-pulsed targets, and the lysis was inhibited by concanamycin A. We also detected these peptide-specific cytolytic and IFN-gamma-producing CD4(+) T cells in short-term bulk cultures of PBMC from each of the three VV-immune donors tested. These are the first VV-specific CD4(+) T cell epitopes identified in humans restricted by one of the most common MHC class II molecules, HLA-DR1, and this information may be useful in analyzing CD4(+) T cell responses to pre-existing or new generation VV vaccines against smallpox.
Efficient Transformation System for <i>Propionibacterium freudenreichii</i> Based on a Novel Vector (2001)

A 3.6-kb endogenous plasmid was isolated from a Propionibacterium freudenreichii strain and sequenced completely. Based on homologies with plasmids from other bacteria, notably a plasmid from Mycobacterium, a region harboring putative replicative functions was defined. Outside this region two restriction enzyme recognition sites were used for insertion of an Escherichia coli-specific replicon and an erythromycin resistance gene for selection in Propionibacterium. Hybrid vectors obtained in this way replicated in both E. coli and P. freudenreichii. Whereas electroporation of P. freudenreichii with vector DNA isolated from an E. coli transformant yielded 10 to 30 colonies per microg of DNA, use of vector DNA reisolated from a Propionibacterium transformant dramatically increased the efficiency of transformation (> or =10(8) colonies per microg of DNA). It could be shown that restriction-modification was responsible for this effect. The high efficiency of the system described here permitted successful transformation of Propionibacterium with DNA ligation mixtures.
An Inverse Heat Transfer Approach to Mitigating Sources of Experimental Error in Transient Heat Transfer Experiments (2013)

The Inverse Flux Solver for Arbitrary Waveforms (IFSAW) algorithm is a transient, simultaneous solution of time resolved adiabatic effectiveness, η(t), and heat transfer coefficient, h(t). Numerical simulations showed IFSAW maintained its high accuracy despite two experimental sources of error typically found when using a transient heat transfer method. The traditional transient method involves exposing a film cooled wind tunnel model at uniform temperature to a step change in freestream temperature. The experimental design results in nearly one-dimensional heat transfer and allows the surface to be modeled as semi-infinite. Typically, the surface temperature history is correlated to an analytical solution to the governing heat transfer equation (yielding η and h), but the required temperature step change is impossible to achieve in a laboratory. This paper first analyzed the error introduced by imperfect step changes and evaluated an alternative methodology, IFSAW, requiring only an arbitrary change in freestream temperature occurring at any rate. Secondly, severe error in h (found in locations where η is near unity because the surface temperature changes little from the initial temperature) was shown to be mitigated using IFSAW combined with a gradual change in coolant temperature at any point during measurement. With both complications, IFSAW maintains its ability to determine periodic η(t) and h(t) waveforms. In these ways, IFSAW is shown to be superior to the legacy transient method.
EXCITATION COUPLING OF <font>Au/ZnO</font> BAND-GAP ENERGY FOR ENHANCING THE PERFORMANCE OF SURFACE PLASMON RESONANCE BIOSENSOR (2013)

This paper reports a novel design of a surface plasmon resonance (SPR) device that can be developed into a high-performance biosensor. The design utilizes wurtzite crystal structure semiconductor (zinc oxide, ZnO) layers to enhance the SPR signal quality and to improve the full width at half maximum (FWHM) of the SPR reflectivity curve. There exists a new mechanism when the excitation in the plasmon energy is in resonance with the ZnO band-gap (BG) energy transfer process. The excitation energy between Au and ZnO causes an interactive, coupled phenomenon with the ZnO BG (λ BG = 360–430 nm, 3.1–3.4 eV) when it matches the ZnO–Au interface surface plasmon (400 nm). We have optimized the design of the ZnO and Au thin films thicknesses through analytical comparisons with conventional SPR structures. Upon exposure to ethanol, the ZnO–Au device showed a 2-times decrease in the FWHM and a 4.5-times larger shift in intensity interrogation. The ZnO–Au device exhibits a wider linearity range and very higher sensitivity. This characteristic of the ZnO–Au device is the basis for realizing accurate and specific biomolecular detection.
Grain sorghum response to granular formulations of iron sources and hydrophilic polymers (1992)

Abstract Banded hydrogels containing FeSO4 have been shown to provide available Fe for plants on calcareous, Fe‐deficient soils. Because granular mixtures of gel‐forming polymers and Fe sources would be easier to apply to soil than hydrogels, several such granular products were compared with banded hydrogels in greenhouse pot experiments. Several FeSO4 + gel‐forming polymer (polyacryl‐amide) formulations were granulated in a rotary tableting press and band applied to an Fe‐deficient Epping silt loam soil (Ustic Torriorthent). None of the granular formulations of FeSO4 + polymers banded alone or with other additives (urea, ammominum sulfate, or bentonite clay) was effective in providing available Fe to grain sorghum (Sorghum bicolor L. Moench). However, a granular formulation of FeEDTA + polymer increased sorghum dry matter yields and Fe uptake. Banded hydrated polymer formulations containing either FeEDTA or FeSO4 also were effective in providing available Fe to sorghum. Results of an associated laboratory experiment showed that some gel formation occurred at granule sites of the FeSO4 + polymer formulations within one week after soil application. Apparently, gel formation was inadequate to affect Fe reactions with the soil to form unavailable products. Limited gel formation with granular products may have resulted from insufficient free water in soil, high solute concentrations in the soil solution surrounding the granules, or hard granules. These results suggest that application of granular products of FeSO4 with polymers may not be an effective method of soil application unless other polymers, additives, or ratios of polymers, additives, and FeSO4 can be identified.
Effects of a novel weight‐loss combination product containing orlistat and acarbose on obesity: A randomized, placebo‐controlled trial (2022)

This trial indicates that orlistat and acarbose can be successfully combined in a modified-release formulation to provide efficacious weight loss with no unexpected safety issues. EMP16 may be a promising candidate among other medications for improved weight management.