gebhart/patrepeval huggingface dataset:
IMMORTALIZED HUMAN HEPATOCYTES (2001)

Acetaminophen (APAP) overdose, whether deliberate or accidental is a common cause of acute liver failure. In recent years, porcine hepatocyte-based extracorporeal liver support has been used successfully to treat APAP induced liver failure, allowing complete patient recovery without requiring liver transplantation. However, porcine hepatocytes may cause immunogenic complications and raise concerns relative to zoonotic disease transmission, e.g., porcine endogenous retroviruses. As an altemative, we have developed immortalized human hepatocyte cell lines, which express high levels of APAP detoxification enzymes. These cell lines were derived by liposome-mediated immortalization of primary hepatocytes from a 12-year-old female donor liver with the SV40 T antigen and have been maintained in proprietary media through passages ranging from 15 to 36. They express cytokeratins 8 and 18, liver specific transcription factors as well as albumin. Certain cytochrome p450 isozymes have been detected by RT-PCR analysis along with P450 dealkylase activity. APAP metabolites were detected by HPLC after 24 hours incubation with the substrate. Both early and late passage cells actively metabolized APAP, typically with production of the sulfate conjugate >glucuronide >mercapturate.TableComparative analysis revealed that total APAP metabolite production by these cell lines is similar to or higher than production by primary human heoatocytes. In summary, the ability to continuously propagate and cryopreserve immortalized human hepatocytes that reproducibly metabolize APAP, suggests that these cells offer a commercially viable altemative to the use of porcine hepatocytes for extracorporeal liver support. This work was funded in part by NIST-ATP award # 70NANB7H3070 and MultiCell Associates, Inc., Warwick, Rl.
High affinity zoledronate-based metal complex nanocrystals to potentially treat osteolytic metastases (2022)

Formation of nano -Ca@ZOLE with viable properties in terms of structure, dissolution, stability, binding, and cytotoxicity, to render it suitable for osteolytic metastasis therapy.
Sensing atmospheric water vapor with the global positioning system (1993)

Global Positioning System (GPS) receivers, water vapor radiometers (WVRs), and surface meteorological equipment were operated at both ends of a 50‐km baseline in Colorado to measure the precipitable water vapor (PWV) and wet delay in the line‐of‐sight to GPS satellites. Using high precision orbits, WVR‐measured and GPS‐inferred PWV differences between the two sites usually agreed to better than 1 mm. Using less precise on‐line broadcast orbits increased the discrepancy by 30%. Data simulations show that GPS measurements can provide mm‐level separate PWV estimates for the two sites, as opposed to just their difference, if baselines exceed 500 km and the highest accuracy GPS orbits are used.
Anti-tumor Polysaccharide from<i>Lactobacillus</i>sp. (1983)

Journal Article Anti-tumor Polysaccharide from Lactobacillus sp. Get access Munehiro Oda, Munehiro Oda Research Institute of Meiji Milk Products Co. Ltd., 1–21–3 Sakae-cho, Higashimurayama-shi, Tokyo 189, Japan Search for other works by this author on: Oxford Academic Google Scholar Hideo Hasegawa, Hideo Hasegawa Research Institute of Meiji Milk Products Co. Ltd., 1–21–3 Sakae-cho, Higashimurayama-shi, Tokyo 189, Japan Search for other works by this author on: Oxford Academic Google Scholar Sumiko Komatsu, Sumiko Komatsu Research Institute of Meiji Milk Products Co. Ltd., 1–21–3 Sakae-cho, Higashimurayama-shi, Tokyo 189, Japan Search for other works by this author on: Oxford Academic Google Scholar Michio Kambe, Michio Kambe Research Institute of Meiji Milk Products Co. Ltd., 1–21–3 Sakae-cho, Higashimurayama-shi, Tokyo 189, Japan Search for other works by this author on: Oxford Academic Google Scholar Fumiyasu Tsuchiya Fumiyasu Tsuchiya Research Institute of Meiji Milk Products Co. Ltd., 1–21–3 Sakae-cho, Higashimurayama-shi, Tokyo 189, Japan Search for other works by this author on: Oxford Academic Google Scholar Agricultural and Biological Chemistry, Volume 47, Issue 7, 1 July 1983, Pages 1623–1625, https://doi.org/10.1080/00021369.1983.10865826 Published: 01 July 1983 Article history Received: 10 June 1982 Published: 01 July 1983
Analysis and improvement of the vector quantization in SELP (Stochastically Excited Linear Prediction) (1988)

The Stochastically Excited Linear Prediction (SELP) algorithm is described as a speech coding method employing a two-stage vector quantization. The first stage uses an adaptive codebook which efficiently encodes the periodicity of voiced speech, and the second stage uses a stochastic codebook to encode the remainder of the excitation signal. The adaptive codebook performs well when the pitch period of the speech signal is larger than the frame size. An extension is introduced, which increases its performance for the case that the frame size is longer than the pitch period. The performance of the stochastic stage, which improves with frame length, is shown to be best in those sections of the speech signal where a high level of short-term correlations is present. It can be concluded that the SELP algorithm performs best during voiced speech where the pitch period is longer than the frame length.
Development of a Functional Monoclonal Single-Chain Variable Fragment Antibody Against Venezuelan Equine Encephalitis Virus (1999)

We have generated a single-chain variable fragment (ScFv) antibody, from a previously well-characterized monoclonal antibody (MAb) to Venezuelan equine encephalitis (VEE) virus, 5B4D-6. The variable regions of the heavy (V(H)) and light (V(L)) chain antibody genes, were connected by a DNA linker and cloned in the phagemid vector pCANTAB5E. The ScFv clone in Escherichia coli strain TG-1, 5B4D-6-6, was expressed as a approximately 30 kDa ScFv protein and higher molecular weight fusion products which were functional in recognizing VEE virus by enzyme-linked immunosorbent assay (ELISA). Results were reproduced in Escherichia coli strain HB2151, where clone D66 was expressed mainly as soluble periplasmic protein. The D66 ScFv antibody bound VEE virus strongly as determined by ELISA. Nucleotide sequence analysis of 5B4D-6-6 ScFv indicated that the Vkappa gene belonged to family XVI, subgroup V, while the V(H) gene was unique in its sequence, though its amino acid sequence could be subgrouped as IA. The deduced protein sequence of D66 was highly homologous to published murine ScFv protein sequences. This work demonstrates, for the first time, cloning of a functional ScFv antibody against VEE virus.
Proton Exchange Membranes for Fuel Cell Applications (2006)

This paper presents an overview of the key requirements for the proton exchange membranes (PEM) used in fuel cell applications, along with a description of the membrane materials currently being used and their ability to meet these requirements. Also discussed are some of the new materials, technologies, and research directions being pursued to try to meet the demanding performance and durability needs of the PEM fuel cell industry.
Digitally assisted equalization of third-order intermodulation products in wideband direct conversion receivers (2009)

An effective linearization technique capable of equalizing IM3 products resulting from an arbitrary out-of-band blocking scenario in a wideband direct conversion receiver is presented. IM3 products are regenerated in the RF analog domain of a low-power mixed-signal feedforward path and are used to cancel analogous signal terms in the original receiver at digital baseband via adaptive equalization. The composite SAW-less receiver achieves an improvement in effective IIP3 from −7.1 to +5.3 dBm under worst-case UMTS Region 1 blocking when the feedforward path is active.
USING MILITARY NAVIGATION RADIOS TO ENHANCE TRANSIT DISPATCHING SYSTEMS (1995)

Via the federal Technology Reinvestment Program, an existing military integrated position-location and communication system based on networked spread-spectrum radios is being adapted to satisfy safety-critical operational requirements for train location and control in railroad and transit applications. Radios installed on trains, at wayside locations, and at control stations, participate in a synchronous time-slotted network, enabling location determination in conjunction with reliable transfer of control information.
Psychophysics, reliability, and norm values for temporal contrast sensitivity implemented on the two alternative forced choice C-Quant device (2011)

The current paper describes the design and population testing of a flicker sensitivity assessment technique corresponding to the psychophysical approach for straylight measurement. The purpose is twofold: to check the subjects' capability to perform the straylight test and as a test for retinal integrity for other purposes. The test was implemented in the Oculus C-Quant straylight meter, using homemade software (MATLAB). The geometry of the visual field lay-out was identical, as was the subjects' 2AFC task. A comparable reliability criterion ("unc") was developed. Outcome measure was logTCS (temporal contrast sensitivity). The population test was performed in science fair settings on about 400 subjects. Moreover, 2 subjects underwent extensive tests to check whether optical defects, mimicked with trial lenses and scatter filters, affected the TCS outcome. Repeated measures standard deviation was 0.11 log units for the reference population. Normal values for logTCS were around 2 (threshold 1%) with some dependence on age (range 6 to 85 years). The test outcome did not change upon a tenfold (optical) deterioration in visual acuity or straylight. The test has adequate precision for checking a subject's capability to perform straylight assessment. The unc reliability criterion ensures sufficient precision, also for assessment of retinal sensitivity loss.
Metalloboranes from first-principles calculations: A candidate for high-density hydrogen storage (2015)

Using first principles calculations, we show the high hydrogen storage capacity of a new class of compounds, metalloboranes. Metalloboranes are transition metal (TM) and borane compounds that obey a novel-bonding scheme. We have found that the transition metal atoms can bind up to 10 H2 molecules.
Very long wavelength In<i>x</i>Ga1−<i>x</i>As/GaAs quantum well infrared photodetectors (1994)

We demonstrate a long wavelength (λc=20 μm) quantum well infrared photodetector using nonlattice matched InxGa1−xAs/GaAs materials system. High optical gains (low capture probabilities) were achieved by using GaAs as a barrier material in this system. A detectivity of D*=9.7×1010 cm√Hz/W at T=10 K has been achieved.
Synthesis Of B, B', B"-Trichloroborazine (1988)

Simplified, relatively safe, and economical synthesis of B, B', B-trichloroborazine easily practiced in standard organic-chemistry laboratory. Yield is 20 to 30 percent, fairly acceptable value in view of inherent difficulty of synthesizing borazines. New synthesis has potential use in industry.
Small Molecule Agonists for the Neurotensin 1 Receptor (NTR1 Agonists) (2013)

A non-high throughput screening (HTS) scaffold-hop program identified the probe candidate ML301 and associated analogs. ML301 met probe nomination criteria, exhibiting full agonist behavior (79 – 93%) with an half maximal effective concentration (EC50) of 2.0 – 4.1 μM against neurotensin 1 receptor (NTR1) in the primary assay. This probe satisfied our secondary assay requirements; namely, the Ca2+ mobilization Fluorescence Imaging Plate Reader (FLIPR) assay (93% efficacy at 298 nM) and good selectivity relative to NTR2 and G protein-coupled receptor (GPR)35. In further profiling, ML301 showed low potential for promiscuity and improved pharmacological data when compared to existing art. A scaffold hop program was initiated in parallel with the originally planned high throughput screening based approach. This approach identified new scaffolds of which the most promising was a quinazoline derivative represented by the singleton MLS-0233108. Medicinal chemistry optimization of MLS-0233108 led to ML314, the most potent molecule in this second series that exhibited full agonist behavior (100 %) on NTR1 (EC50 = 1.9 μM). ML314 showed good selectivity against NTR2 and GPR35, but did not stimulate Ca2+ mobilization. ML314 is potentially a biased agonist operating via the β-arrestin pathway rather than the traditional Gq coupled pathway. Signaling mediated by β-arrestin has distinct biochemical and functional consequences that may lead to physiological advantages as described below. This probe report describes the discovery and properties of ML301 and summarizes the HTS and follow-up campaign, which identified ML314.
Signature based diagnosis for logic BIST (2007)

This paper presents a new approach for performing logic BIST diagnosis exclusively using MISR signatures. Unlike conventional logic BIST diagnosis approaches which require either huge test time or complicated logic BIST design and ATE flow, signature based diagnosis does not require dynamically changing MISR operations for each failing device. Our experimental data shows that signature based diagnosis can achieve similar diagnosis resolution with manageable diagnosis run time while eliminating most of the complexity associated with the traditional approach to logic BIST diagnostics.
Ultrasonic Doppler Technique for Imaging Blood Vessels (1972)

Present ultrasonic Doppler flow detectors that use the Doppler effect on waves scattered from moving blood have provided useful information when directed by hand to trace the circulation of animals and man. By scanning with a highly directive flow detector, the areas of flow can be localized. Images can be formed of the interior of blood vessels. These images have the appearance of arteriograms and venograms made by dye contrast radiography, but have none of its hazards. The resolution appears adequate for useful images.
Novel Method to Enhance Sternal Healing After Harvesting Bilateral Internal Thoracic Arteries With Use of Basic Fibroblast Growth Factor (2000)

Background —Poor healing of the sternum often limits the use of bilateral internal thoracic arteries (BITAs) in coronary bypass surgery, especially for diabetic patients. We have reported that basic fibroblast growth factor (bFGF) enhanced regeneration of the skull. This study was designed to evaluate the effects of topical use of bFGF on sternal healing after removing the BITAs. Methods and Results —Forty-five Wistar rats were subjected to median sternotomy and were divided into 3 groups: 15 had the BITAs removed and had a bFGF sheet applied on the posterior table of the sternum (group A), 15 had just the BITAs removed (group B), and 15 had intact BITAs (group C). Five and 10 rats were euthanized 2 and 4 weeks after surgery, respectively, in all 3 groups. Peristernal blood flow, measured with use of a noncontact laser flowmeter, decreased after removal of the BITAs ( P <0.001). Four weeks after the surgery, PBF markedly increased only in group A (9.7±1.2, 6.5±0.6, and 8.2±0.5 mL · min −1 · 100 g −1 for groups A, B, and C, respectively; P <0.01 by ANOVA). Four weeks after surgery, the following findings were obtained only in group A: (1) nearly completely healed sternum filled with regenerated bone tissue, (2) marked angiogenesis around the sternum, and (3) osteoblasts in an active form around the edge of the sternum. Conclusions —The results suggest that use of the bFGF sheet offset the sternal ischemia and accelerated sternal healing. This method may help to decrease sternal necrosis in high-risk patients or allow extended use of BITAs in coronary bypass surgery.
High Performance Zn-Based Batteries Using 3D Monolithic Sponge Anodes (2013)

Abstract not Available.
Visualisations compactes (2002)

We introduce a descriptive model that allows the definition of a large class of information visualization algorithms with a small number of parameters. Compact visualizations, which we conjecture is equivalent to the class of visualizations that can be rendered in a time directly proportional to the size of the input data, are defined by a fixed dataflow architecture: clustering and subclustering of input data, sort, graphic primitives and graphic attributes generation. At each step, the parameters are expressions of the host programming language, which include input attribute names and local variable names. Local variables are a specific concept that allows us to extend the expressiveness of the dataflow architecture. We introduce our model formally, and then show its expressiveness with a few examples.
Exploring the design space of multiscale 3D orientation (2010)

Recently, research in 3D computer graphics and interaction has started to move beyond the narrow domain of single object authoring and inspection, and has begun to consider complex multiscale objects and environments. This generalization of problem scope calls for more general solutions, which are more akin to information visualization techniques than traditional computer graphics approaches.
Metal–organic frameworks—prospective industrial applications (2005)

The generation of metal–organic framework (MOF) coordination polymers enables the tailoring of novel solids with regular porosity from the micro to nanopore scale. Since the discovery of this new family of nanoporous materials and the concept of so called ‘reticular design’, nowadays several hundred different types of MOF are known. The self assembly of metal ions, which act as coordination centres, linked together with a variety of polyatomic organic bridging ligands, results in tailorable nanoporous host materials as robust solids with high thermal and mechanical stability.
Functionally diverse heteromeric traps for ligands of the transforming growth factor-β superfamily (2021)

Abstract Ligands of the transforming growth factor-β (TGF-β) superfamily are important targets for therapeutic intervention but present challenges because they signal combinatorially and exhibit overlapping activities in vivo. To obtain agents capable of sequestering multiple TGF-β superfamily ligands with novel selectivity, we generated soluble, heterodimeric ligand traps by pairing the extracellular domain (ECD) of the native activin receptor type IIB (ActRIIB) alternately with the ECDs of native type I receptors activin receptor-like kinase 4 (ALK4), ALK7, or ALK3. Systematic analysis of these heterodimeric constructs by surface plasmon resonance, and comparison with their homodimeric counterparts, revealed that each type I receptor partner confers a distinct ligand-binding profile to the heterodimeric construct. Additional characterization in cell-based reporter gene assays confirmed that the heterodimeric constructs possessed different profiles of signaling inhibition in vitro, which translated into altered patterns of pharmacological activity when constructs were administered systemically to wild-type mice. Our results detail a versatile platform for the modular recombination of naturally occurring receptor domains, giving rise to inhibitory ligand traps that could aid in defining the physiological roles of TGF-β ligand sets or be directed therapeutically to human diseases arising from dysregulated TGF-β superfamily signaling.
Design and Synthesis of Thiazolo[5,4-f]quinazolines as DYRK1A Inhibitors, Part I (2014)

The convenient synthesis of a library of novel 6,6,5-tricyclic thiazolo[5,4-f] quinazolines (forty molecules) was achieved mainly under microwave irradiation. Dimroth rearrangement and 4,5-dichloro-1,2,3,-dithiazolium chloride (Appel salt) chemistry were associated for the synthesis of a novel 6-aminobenzo[d]thiazole-2,7-dicarbonitrile (16) a versatile molecular platform for the synthesis of various bioactive derivatives. Kinase inhibition of the final compounds was evaluated on a panel of four Ser/Thr kinases (DYRK1A, CDK5, CK1 and GSK3) chosen for their strong implications in various regulation processes, especially Alzheimer’s disease (AD). In view of the results of this preliminary screening, thiazolo[5,4-f]quinazoline scaffolds constitutes a promising source of inspiration for the synthesis of novel bioactive molecules. Among the compounds of this novel chemolibrary, 7i, 8i and 9i inhibited DYRK1A with IC50 values ranging in the double-digit nanomolar range (40, 47 and 50 nM, respectively).
Optical component: nonreciprocal three-way divider based on magneto-optical resonator (2013)

We suggest and analyze a new compact nonreciprocal optical component based on a magneto-optical (MO) resonator. This component fulfills simultaneously two functions, namely, equal division of the input signal between three output ports and isolation of the input port from output ones. Using group theory, we analyze the scattering matrix of this symmetrical component. Our numerical results for one of the possible schemes of the divider based on 2D photonic crystal with MO material demonstrate that, at the central frequency, the division of the signal between the three output ports is about -6.4 dB. The variation of the division levels in the output ports in this band is (-6.4±0.4) dB. For two of the output ports, the calculated bandwidth for the level -20 dB of the isolation is around 219 GHz at the wavelength 1.55 μm. For the third output port, the isolation at the central frequency is about -6 dB.
Nonlinear propagation delay and pulse distortion resulting from dual frequency band transmit pulse complexes (2011)

A method of acoustic imaging is discussed that potentially can improve the diagnostic capabilities of medical ultrasound. The method, given the name second order ultrasound field imaging, is achieved by the processing of the received signals from transmitted dual frequency band pulse complexes with at least partly overlapping high frequency (HF) and low frequency (LF) pulses. The transmitted HF pulses are used for image reconstruction whereas the transmitted LF pulses are used to manipulate the elastic properties of the medium observed by the HF imaging pulses. In the present paper, nonlinear propagation effects observed by a HF imaging pulse due to the presence of a LF manipulation pulse is discussed. When using dual frequency band transmit pulse complexes with a large separation in center frequency (e.g., 1:10), these nonlinear propagation effects are manifested as a nonlinear HF propagation delay and a HF pulse distortion different from conventional harmonic distortion. In addition, with different transmit foci for the HF and LF pulses, nonlinear aberration will occur.
Sequencing of Configuration Operations for IP Networks (2000)

When configuring an IP network, changes may need to be made to several devices as part of one configuration operation. Given an in-band access to the devices where data and control packets flow on the same network, it becomes imperative to sequence the changes intelligently to retain connectivity to the devices that need to be reconfigured. In this paper, we present techniques to sequence configuration operations for IP networks. We describe our experiences with implementation of the techniques, and experimentally evaluate the usefulness of the proposed techniques. We demonstrate that automatic sequencing is valuable in IP network configuration.
The proteolytic activation of interleukin-1 beta. (1991)

Interleukin-1 beta (IL-1 beta) is released from an inactive precursor by a proteolytic cleavage. A monocytic protease has been identified that appears to be involved in the physiological activation of this cytokine. Two situations have been found in which precursor IL-1 beta exists without the monocytic processing enzyme, and in these cases other proteases, such as neutrophil elastase, cathepsin G and cathepsin L, may be involved in generating the active cytokine.
Novel chemistry of taxol. Retrosynthetic and synthetic studies (1994)

K. C. Nicolaou, P. G. Nantermet, H. Ueno and R. K. Guy, J. Chem. Soc., Chem. Commun., 1994, 295 DOI: 10.1039/C39940000295