gebhart/patrepeval huggingface dataset:
Characterization of the binding of [<sup>3</sup>H]‐clobenpropit to histamine H<sub>3</sub>‐receptors in guinea‐pig cerebral cortex membranes (1999)

1 We have investigated the binding of a novel histamine H3-receptor antagonist radioligand, [3H]- clobenpropit ([3H]-VUF9153), to guinea-pig cerebral cortex membranes. 2 Saturation isotherms for [3H]-clobenpropit appeared biphasic. Scatchard plots were curvilinear and Hill plot slopes were significantly less than unity (0.63+/-0.03; n = 12+/-s.e.mean). The radioligand appeared to label two sites in guinea-pig cerebral cortex membranes with apparent affinities (pKD') of 10.91+/-0.12 (Bmax = 5.34+/-0.85 fmol mg(-1) original wet weight) and 9.17+/-0.16 (Bmax = 23.20+/-6.70 fmol mg(-1)). 3 In the presence of metyrapone (3 mM) or sodium chloride (100 mM), [3H]-clobenpropit appeared to label a homogeneous receptor population (Bmax=3.41+/-0.46 fmol mg-1 and 3.49+/-0.44 fmol mg(-1), pKD' = 10.59+/-0.17 and 10.77+/-0.02, respectively). Scatchard plots were linear and Hill slopes were not significantly different from unity (0.91+/-0.04 and 0.99+/-0.02, respectively). Granisetron (1 microM), rilmenidine (3 microM), idazoxan (0.3 microM), pentazocine (3 microM) and 1,3-di-(2-tolyl)guanidine (0.3 microM) had no effect on the binding of [3H]-clobenpropit. 4 The specific binding of [3H]-clobenpropit appeared to reach equilibrium after 25 min at 21+/-3 degrees C and remained constant for >180 min. The estimated pKD' (10.27+/-0.27; n = 3+/-s.e.mean) was not significantly different from that estimated by saturation analysis in the presence of metyrapone. 5 A series of histamine H3-receptor ligands expressed affinity values for sites labelled with [3H]-clobenpropit which were not significantly different from those estimated when [3H]-R-alpha-MH was used to label histamine H3-receptors in guinea-pig cerebral cortex membranes.
Secure smartphone-based registration and key deployment for vehicle-to-cloud communications (2013)

Intelligent Transportation Systems that rely on Vehicle-to-Cloud communications are emerging. Of particular interest to us is the question how drivers can securely register their vehicle with cloud services and deploy keys for securing vehicular communications. We therefore present a secure smartphone-based registration and key deployment process for these applications. We combine an adapted OAuth flow for Smartphone-to-Cloud communications with a novel key deployment mechanism for Public Key Infrastructures in vehicular networks. The primary contributions of the proposed key deployment process are a high degree of independence from central authorities and feasible security audits due to an open protocol design. As an instance of future Intelligent Transportation System applications that facilitate Vehicle-to-Cloud communications, we utilize the V-Charge project as a running example to discuss potential attack scenarios and their mitigations.
Citrate alterations in primary and metastatic human prostatic adenocarcinomas: <sup>1</sup>H magnetic resonance spectroscopy and biochemical study (1993)

The objectives of this study were to quantitatively verify the low levels of citrate previously observed in primary human prostatic adenocarcinomas and to determine whether citrate was further reduced in metastatic prostatic cancer. This was accomplished by comparison of citrate concentrations of DU 145 xenografts (a poorly differentiated human prostatic adenocarcinoma cell line grown in nude mice) with concentrations in primary human adenocarcinomas. Following in vivo 1H NMR studies of DU 145 xenografts, citrate concentrations of DU 145 xenografts and surgically removed primary prostatic adenocarcinoma tissue were determined by quantitative high resolution 1H NMR and enzymatic assay. The most significant findings of this study were that citrate concentrations in primary human adenocarcinomas (3.74 +/- 0.19 mumol/g wet weight) were significantly lower than those observed for normal and benign hyperplastic (BPH) prostatic tissues. Furthermore there was a further ten-fold reduction of citrate associated with DU 145 xenografts (0.31 +/- 0.028 mumole/g wet weight) compared with primary prostatic cancer. DU 145 xenografts also exhibited higher levels of uridine diphosphosugars and choline containing metabolites relative to primary prostatic adenocarcinomas. These findings support the hypothesis that citrate is low in primary prostatic cancer and further reduced in metastatic disease.
Using data clustering to improve cleaning performance for flash memory (1999)

Flash memory offers attractive features for storage of data, such as non-volatility, shock resistance, fast access speed, and low power consumption. However, it requires erasing before it can be overwritten. The erase operations are slow and consume comparatively a great deal of power. Furthermore, flash memory can only be erased a limited number of times. To overcome hardware limitations, we use the non-in-place update mechanism that requires a cleaner to reclaim space occupied by obsolete data. To improve cleaning performance and prolong flash memory lifetime, we propose a new data reorganization method. By this method, data in flash memory are dynamically classified and clustered together according to their accessing frequencies. Experimental results show that this clustering technique significantly improved the cleaning performance for a variety of cleaning algorithms. The number of erase operations performed is greatly reduced and flash memory lifetime is prolonged. Even wearing is ensured as well. Copyright © 1999 John Wiley & Sons, Ltd.
Depth map estimation from single-view image using object classification based on Bayesian learning (2010)

Generation of three-dimensional (3D) scenes from two-dimensional (2D) images is an important step for a successful introduction to 3D multimedia services. Among the relevant problems, depth estimation from a single-view image is probably the most difficult and challenging task. In this paper, we propose a new depth estimation method using object classification based on the Bayesian learning algorithm. Using training data of six attributes, we categorize objects in the single-view image into four different types. According to the type, we assign a relative depth value to each object and generate a simple 3D model. Experimental results show that the proposed method estimates depth information properly and generates a good 3D model.
We Can Track You If You Take the Metro: Tracking Metro Riders Using Accelerometers on Smartphones (2015)

Motion sensors (e.g., accelerometers) on smartphones have been demonstrated to be a powerful side channel for attackers to spy on users' inputs on touchscreen. In this paper, we reveal another motion accelerometer-based attack which is particularly serious: when a person takes the metro, a malicious application on her smartphone can easily use accelerator readings to trace her. We first propose a basic attack that can automatically extract metro-related data from a large amount of mixed accelerator readings, and then use an ensemble interval classier built from supervised learning to infer the riding intervals of the user. While this attack is very effective, the supervised learning part requires the attacker to collect labeled training data for each station interval, which is a significant amount of effort. To improve the efficiency of our attack, we further propose a semi-supervised learning approach, which only requires the attacker to collect labeled data for a very small number of station intervals with obvious characteristics. We conduct real experiments on a metro line in a major city. The results show that the inferring accuracy could reach 89\% and 92\% if the user takes the metro for 4 and 6 stations, respectively.
Predicting origami-inspired programmable self-folding of hydrogel trilayers (2016)

Imitating origami principles in active or programmable materials opens the door for development of origami-inspired self-folding structures for not only aesthetic but also functional purposes. A variety of programmable materials enabled self-folding structures have been demonstrated across various fields and scales. These folding structures have finite thickness and the mechanical properties of the active materials dictate the folding process. Yet formalizing the use of origami rules for use in computer modeling has been challenging, owing to the zero-thickness theory and the exclusion of mechanical properties in current models. Here, we describe a physics-based finite element simulation scheme to predict programmable self-folding of temperature-sensitive hydrogel trilayers. Patterning crease and assigning mountain or valley folds are highlighted for complex origami such as folding of the Randlett's flapping bird and the crane. Our efforts enhance the understanding and facilitate the design of origami-inspired self-folding structures, broadening the realization and application of reconfigurable structures.
Effect of Planting Date and Nitrogen Fertilization Rates on No-till Pumpkins (2008)

Vegetable growers in the Mountain region of North Carolina are faced with increased land prices resulting from urbanization and reduced farm income from low-commodity prices. Local consumer use of pumpkin ( Cucurbita pepo ) for jack-o-lanterns and baking provides a fall market for growers to increase production and profitability on-farm. Most soils in these regions are highly erodible and susceptible to drought during the growing season. Little information is available on cultural practices for no-till pumpkin production in this region. Field studies were established to evaluate the yield response of no-till pumpkin to planting date and nitrogen (N) fertilization. Experiments were conducted at the Mountain (MRS), Upper Mountain (UMRS), and the Mountain Horticultural Crops Research Stations (MHCRS) in Summer 2003 and 2004 using no-till cultural practices. Three planting dates were established at 2-week intervals and 0, 40, 80, and 120 kg·ha −1 N treatments were applied at each planting date in a randomized complete block design. The 80 and 120 kg·ha −1 N fertilization rates produced greater yields and larger fruit size than the 0 and 40 kg·ha −1 N rates. Pumpkins planted earliest produced the greatest marketable and total yields for all N rates at all three locations. The latest planting date (9 July) and highest N rate yielded more cull fruit compared with marketable pumpkins with the earlier planting date at the Upper Mountain Research Station. This location has a shorter growing season and cooler summer temperatures than the two other locations. Although the third planting date was late for pumpkin planting, higher N rate treatments at that timing produced marketable yields comparable to earlier planting dates at the two warmer summer locations (MRS and MHCRS). In these experiments, the highest rate applied (120 kg·ha −1 N) maximized pumpkin yield. This observation would indicate that higher yields might be possible with even greater N rates.
Development of a Practical Model of Partnering for Construction Projects (2004)

This paper presents a practical model of partnering, which integrates the processes and associated components for partnering espousal. It intends to use the concept of modeling to integrate the aspects of system and process to form a model for practicing partnering. Therefore, a system-process model that is called the procedural mapping model (PMM) is developed, which adopts a more systematic approach to modeling construction partnering, including the identification of the core practices and activities. The PMM forms the basis for designing the key elements of the practical model of partnering that involves three major establishments including interactive process description, success factors’ monitoring, and goals’ assessment matrices. Individual components of these establishments have also been described.
Stability of PRV Resistance in Transgenic Papaya (1995)

Transgenic papaya lines carrying the coat protein gene (CP) of papaya ringspot virus (PRV) strain HA 5-1 display PRV reactions ranging from complete susceptibility (39-3 & 39-4), to slight delay in onset of symptoms (39-1) and attenuation of symptoms (60-3), to high-level resistance (55-1, 63-1). Normal Mendelian segregation of transgene expression was lost in R1 of 39-3 and 39-4, and inbred R1 60-3 gave an aberrant 1:1 ratio. R0 55-1 plants were resistant in the field (Hawaii) for 2 years following manual and/or aphid inoculation, and the high-level resistance remained stable in the R1 after repeated manual inoculations in the greenhouse and graft inoculation for up to 1 year (Cornell). However, inoculation with PRV HA-Oahu strain produced symptoms in some plants at Cornell (9% after 6 weeks) and in Hawaii (50% after 1 year). Two 55-1 and one 60-3 plant subsequently underwent remission of symptoms and became ELISA-negative (Hawaii). Transmission of PRV isolates from symptomatic 55-1 plants to other CP+ 55-1 bioassay plants was unsuccessful.
Third-generation mobile telecommunications and virtual home environment: A prioritization analysis (2002)

The International Telecommunication Union (ITU) is currently setting standards for the third-generation International Mobile Telecommunication 2000 (IMT-2000) system. This system offers its users mobile multimedia services with a global roaming capability. This capability allows IMT-2000 users to invoke the services they have subscribed to with their home network service provider anywhere around the globe in a manner transparent to them. The requirements for global roaming relate to three distinct functional capabilities of the network: mobility management, call/connection control, and service transportability. In this paper we address the role service transportability plays in offering the virtual home environment (VHE) to IMT-2000 users. To achieve the IMT-2000 global roaming capability, service transportability combines the concept of the Intelligent Network's distributed functional plane (DFP) across different IMT-2000 systems with a number of VHE implementation scenarios. We describe the IMT-2000 system and its various VHE implementation scenarios, evaluate those scenarios, and prioritize their deployment with respect to criteria such as location of service control, location of service database, point of service invocation, and required standardization effort.
Synthesis of Stereodefined Polysubstituted Olefins. 1. Sequential Intermolecular Reactions Involving Selective, Stepwise Insertion of Pd(0) into Allylic and Vinylic Halide Bonds. The Stereoselective Synthesis of Disubstituted Olefins<sup>1</sup> (2000)

Palladium-catalyzed allylic substitution and cross-coupling reactions have been combined into a sequential procedure to provide a range of disubstituted olefin products starting from two-, three-, and four-carbon common olefin templates. Diverse application of this template strategy is demonstrated in a variety of model studies and in a parallel synthesis (combinatorial) approach to prepare an allylic amine molecular library. An approach toward the preparation of astaxanthin beta-D-diglucoside, an interesting antioxidant whose total synthesis has yet to be reported, using the olefin-template approach is also discussed.
Emphasized speech synthesis based on hidden Markov models (2009)

This paper presents a statistical approach to synthesizing emphasized speech based on hidden Markov models (HMMs). Context-dependent HMMs are trained using emphasized speech data uttered by intentionally emphasizing an arbitrary accentual phrase in a sentence. To model acoustic characteristics of emphasized speech, new contextual factors describing an emphasized accentual phrase are additionally considered in model training. Moreover, to build HMMs for synthesizing both normal speech and emphasized speech, we investigate two training methods; one is training of individual models for normal and emphasized speech using each of these two types of speech data separately; and the other is training of a mixed model using both of them simultaneously. The experimental results demonstrate that 1) HMM-based speech synthesis is effective for synthesizing emphasized speech and 2) the mixed model allows a more compact HMM set generating more naturally sounding but slightly less emphasized speech compared with the individual models.
Modification of the Gouda Frame to Allow Stereotactic Biopsy of the Brain Using the GE 8800 Computed Tomographic Scanner (1983)

The Gouda stereotactic frame has been modified to interface with the GE 8800 scanner. The patient, attached to the frame, is scanned and then moved to the operating room for a stereotactic biopsy. This is a safe, accurate method of obtaining biopsy specimens of otherwise inaccessible tumors of the brain.
Anchorage of Carbon Fiber Reinforced Polymers to Reinforced Concrete in Shear Applications (2023)

Within the past few decades a new technology has emerged using Fiber Reinforced Polymers (FRP) to rehabilitate and retrofit reinforced concrete (RC) structures. In FRP shear strengthening applications it is largely recognized that debonding is the prevailing failure mode. FRP debonding typically occurs prematurely as a brittle failure mode that limits the efficiency of the strengthening technique. No systematic tests have been conducted to investigate the capacity gained by anchoring FRP laminates to RC elements in shear applications. The objective of this research program was to study the effects of anchoring FRP laminates to RC members with FRP anchors thereby delaying or potentially eliminating debonding of FRP sheets from the concrete surface. FRP anchors used in this research were made from fibers used as part of FRP sheets that get bundled into a roll with a fanned upper end of the anchor allowing the fibers to be splayed over the FRP sheet. A single shear pull test experiment was developed to study the effects of anchoring FRP laminates using FRP anchors with varying anchor diameters, lengths, and patterning. The results of the experimental portion of this research project were used in combination with finite element analyses to develop models for anchored FRP sheets that can be used in design of shear strengthening applications.
In vivo effects of interleukin‐1 on articular cartilage. Prolongation of proteoglycan metabolic disturbances in old mice (1991)

We investigated the effects of intraarticular injections of interleukin-1 alpha (IL-1 alpha) into the knee joints of young (3-month-old) and old (18-month-old) C57Bl/10 mice. In this in vivo study, 35S-sulfate incorporation and release were used to compare the effects of IL-1 on patellar cartilage proteoglycan metabolism. IL-1-induced stimulation of proteoglycan degradation was confined to the first 24 hours after injection in both young and old animals, and was only slightly lower in old cartilage than in young. In old patellar cartilage, IL-1-induced suppression of proteoglycan synthesis appeared to be more prolonged. Also, the amount of time needed to restore the cartilage matrix, characterized by proteoglycan synthesis above normal levels, was longer in old animals. Histologic analysis confirmed the retarded recovery in the cartilage of old mice. Autoradiography showed that the chondrocytes of the medial side of the femorotibial area were most vulnerable to IL-1-induced suppression of proteoglycan synthesis, especially the medial tibial plateau. As with the patellar cartilage, IL-1-induced suppression of proteoglycan synthesis in this area of articular cartilage was more prolonged in old animals. Our data indicated that the impact of IL-1 on articular cartilage is higher in old mice and that, consistent with certain loci found to be at risk in experimental osteoarthritis, there are sites at which IL-1 is more likely to act.
Automated selection and harvesting of pluripotent stem cell colonies (2012)

The ability of pluripotent stem cells to differentiate into specialized cells of all three germ layers, their capability to self-renew, and their amenability to genetic modification provide fascinating prospects for the generation of cell lines for biomedical applications. Therefore, stem cells must increasingly suffice in terms of industrial standards, and automation of critical or time-consuming steps becomes a fundamental prerequisite for their routine application. Cumbersome manual picking of individual stem cell colonies still represents the most frequently used method for passaging or derivation of clonal stem cell lines. Here, we explore an automated harvesting system (CellCelector™) for detection, isolation, and propagation of human embryonic stem cells (hESCs) and murine induced pluripotent stem cells (iPSCs). Automatically transferred hESC colonies maintained their specific biological characteristics even after repeated passaging. We also selected and harvested primary iPSCs derived from mouse embryonic fibroblasts expressing the green fluorescent protein (GFP) under the control of the Oct4 promotor using either morphological criteria or GFP fluorescence. About 80% of the selected and harvested primary iPSC colonies gave rise to homogenously GFP-expressing iPSC lines. To validate the iPSC lines, we analyzed the expression of pluripotency-associated markers and multi-germ layer differentiation potential in vitro. Our data indicate that the CellCelector™ technology enables efficient identification and isolation of pluripotent stem cell colonies at the phase contrast or fluorescence level.