gebhart/patrepeval huggingface dataset:
Bridging the Gap Between Detection and Tracking: A Unified Approach (2019)

Object detection models have been a source of inspiration for many tracking-by-detection algorithms over the past decade. Recent deep trackers borrow designs or modules from the latest object detection methods, such as bounding box regression, RPN and ROI pooling, and can deliver impressive performance. In this paper, instead of redesigning a new tracking-by-detection algorithm, we aim to explore a general framework for building trackers directly upon almost any advanced object detector. To achieve this, three key gaps must be bridged: (1) Object detectors are class-specific, while trackers are class-agnostic. (2) Object detectors do not differentiate intra-class instances, while this is a critical capability of a tracker. (3) Temporal cues are important for stable long-term tracking while they are not considered in still-image detectors. To address the above issues, we first present a simple target-guidance module for guiding the detector to locate target-relevant objects. Then a meta-learner is adopted for the detector to fast learn and adapt a target-distractor classifier online. We further introduce an anchored updating strategy to alleviate the problem of overfitting. The framework is instantiated on SSD and FasterRCNN, the typical one- and two-stage detectors, respectively. Experiments on OTB, UAV123 and NfS have verified our framework and show that our trackers can benefit from deeper backbone networks, as opposed to many recent trackers.
A topographic study on the distribution of cisplatin in xenografted tumors on nude mice (1996)

The intratumoral distribution of cisplatin was studied in terms of platinum concentrations in tissue pieces and immunohistochemically detected cisplatin-DNA adducts in xenografted tumors on nude mice. Heterogeneities in drug distribution were calculated as standard deviations and coefficients of variation. A three-dimensional image of adduct distribution was produced which showed regions with low adduct levels to be topographically connected also in three dimensions. A model was presented for investigating the potential influence of vascularization and cell proliferation on intratumoral adduct distribution by using different immunohistochemical stainings of parallel tissue sections. A weak but significant correlation was found between cisplatin-DNA adducts and proliferation, which might indicate that the drug uptake and adduct formation is increased in proliferating cells.
Large Area Flexible Electronics Fabricated Using Self-Aligned Imprint Lithography (2007)

This paper presents Self-Aligned Imprint Lithography (SAIL), a technology for producing submicron layer-to-layer alignments on large, dimensionally variable flexible plastic structures. The roll-to-roll compatible process is used to make 1 μm channel length transistors and active matrix arrays using a-Si and zinc-tin-oxide (ZTO) with electrical characteristics that nearly match those obtained using photolithography. The remaining issues for commercialization are discussed
Identification of Functional Domains on gClQ-R, a Cell Surface Protein That Binds to the Globular "Heads" of C1Q, Using Monoclonal Antibodies and Synthetic Peptides (1996)

A membrane protein (33 kDa) that binds to the globular "heads" of C1q (gC1q-R) has been recently described. The full length cDNA encoding gC1q-R has been cloned, expressed in E. coli and using the purified recombinant protein (rgC1q-R) as an immunogen, a panel of IgG monoclonal antibodies (MAb) has been produced by fusion of spleen cells from hyperimmunized BALB/c mice with NSO mouse myeloma partners. From this fusion, 60 anti-gC1q-R hybridomas were selected and evaluated for their ability to (1) discriminate between the mature form (MF) of gC1q-R (residues 74-282) and a truncated form (TF) lacking residues 74-95, which contains a major C1q binding site, (2) recognize two functionally defined synthetic peptides derived from the NH2-(XN18) and COOH-(XC15) terminus of gC1q-R, and (3) bind to microtiter well fixed intact Raji cells. Several clones were identified: MAbs 46.23 and 60.11 (IgG1 kappa), reacted strongly with ELISA plate-fixed intact Raji and K562 cells, MF, and the XN18 peptide, but had poor or no reactivity with TF; MAbs 74.5.2 > 25.15 (IgG1 kappa) recognized both MF and TF and are directed against epitopes in the XC15 peptide that contains a binding site for high-molecular-weight kininogen and Factor XII.
Total Internal Reflection Transient Absorption Microscopy: An Online Detection Method for Microfluidics (2020)

Microreactors have garnered widespread attention for their tunability and precise control of synthetic parameters to efficiently produce target species. Despite associated advances, a lack of online detection and optimization methods has stalled the progression of microfluidic reactors. Here we employ and characterize a total internal reflection transient absorption microscopy (TIRTAM) instrument to image excited state dynamics on a continuous flow device. The experiments presented demonstrate the capability to discriminate between different chromophores as well as in differentiating the effects of local chemical environments that a chromophore experiences. This work presents the first such online transient absorption measurements and provides a new direction for the advancement and optimization of chemical reactions in microfluidic devices.
Modelling and control of a double articulated vehicle with four steerable axles (1999)

This paper describes the modelling of a double articulated bus and the controller design for lateral guidance of these vehicles. The bus is assumed to have all wheel steering and independent drives on each wheel of the semi-trailer and the rear-wheels of the tractor. These independent drives together with the steering angles yield 7 possible control inputs. For lateral guidance, 4 inputs are enough. It is shown that the steering angles are the most beneficial inputs. An H/sub /spl infin// controller has been designed to guide the bus along a bus lane. This controller shows good performance under varying road conditions.
Enantioselective Palladium‐Catalyzed [3+2] Cycloaddition of Trimethylenemethane and Fluorinated Ketones (2018)

A nitrile-substituted trimethylenemethane (TMM) donor undergoes palladium-catalyzed [3+2] cycloadditions with fluorinated ketones to generate tetrasubstituted trifluoromethylated centers in high enantioselectivity under mild conditions. The generation of the palladium-TMM complex was achieved by a self-deprotonation strategy, which shows remarkable improvements in regiocontrol, efficiency, and atom economy of asymmetric [3+2] cycloadditions. Moreover, the versatility of the nitrile group provides direct access to a variety of synthetically useful intermediates, including amides, aldehydes, and esters. The developed reaction conditions allow for the synthesis of a wide variety of aromatic, heteroaromatic, and aliphatic fluorinated dihydrofurans in excellent regio- and enantioselectivities.
Laser Refrigeration of Ytterbium‐Doped Sodium–Yttrium–Fluoride Nanowires (2016)

Sodium yttrium fluoride (β-NaYF<sub>4</sub> ) nanowires (NWs) with a hexagonal crystal structure are synthesized using a low-cost hydrothermal process and are shown to undergo laser refrigeration based on an upconversion process leading to anti-Stokes (blueshifted) photoluminescence. Single-beam laser trapping combined with forward light scattering is used to investigate cryophotonic laser refrigeration of individual NWs through analysis of their local Brownian dynamics.
Synthesis of maltosyl(.ALPHA.1.RAR.6)cyclodextrins through the reverse reaction of thermostable Bacillus acidopullulyticus pullulanase. (1989)

Maltosyl(α1→6)α-, β- or γ-cyclodextrin was synthesized from maltose and α-, β- or γ-cyclodextrin, respectively, using Bacillus acidopullulyticus pullulanase (EC 3.2.1.41). More than 40% of each cyclodextrin substrate was converted to the corresponding maltosyl(α1→6)cyclodextrin under the conditions given below; the combined concentration of maltose and cyclodextrin was 70-75% (w/w), the molar ratio of maltose to cyclodextrin was 9-18, and the amount of pullulanase was 100-200units/g of cyclodextrin. The optimum pH and temperature for the formation of maltosyl(α1→6)cyclodextrins were 4.0-4.5 and 60-70°C, respectively. Each maltosyl(α1→6)cyclodextrin produced was separated from noncyclic saccharides, maltose and branched tetraose, by methanol and ethanol precipitations. The maltosyl(α1→6)cyclodextrins were further purified by gel filtration on a Toyopearl HW 40 S column and crystallization from aqueous (for maltosyl(α1→6)α- cyclodextrin) or methanol (for maltosyl(α1→6)β-cyclodextrin) solution. From 10g each of the corresponding cyclodextrin, the yields of the purified maltosyl(α1→6)α-, β- and γ-cylcodextrins were 3.0-3.6g, 2.5-2.8g and 2.2-2.5g, respectively. Identification of the maltosyl(α1→6)cyclodextrins was performed by means of hydrolysis with Klebsiella pneumoniae pullulanase, methylation analysis and 13C-NMR analysis.
Integrated cytokine and metabolite analysis reveals immunometabolic reprogramming in COVID-19 patients with therapeutic implications (2021)

Cytokine release syndrome (CRS) is a major cause of the multi-organ injury and fatal outcome induced by SARS-CoV-2 infection in severe COVID-19 patients. Metabolism can modulate the immune responses against infectious diseases, yet our understanding remains limited on how host metabolism correlates with inflammatory responses and affects cytokine release in COVID-19 patients. Here we perform both metabolomics and cytokine/chemokine profiling on serum samples from healthy controls, mild and severe COVID-19 patients, and delineate their global metabolic and immune response landscape. Correlation analyses show tight associations between metabolites and proinflammatory cytokines/chemokines, such as IL-6, M-CSF, IL-1α, IL-1β, and imply a potential regulatory crosstalk between arginine, tryptophan, purine metabolism and hyperinflammation. Importantly, we also demonstrate that targeting metabolism markedly modulates the proinflammatory cytokines release by peripheral blood mononuclear cells isolated from SARS-CoV-2-infected rhesus macaques ex vivo, hinting that exploiting metabolic alterations may be a potential strategy for treating fatal CRS in COVID-19.
Pro-norm and anti-norm deviance within and between groups. (2000)

Participants evaluated other individuals who deviated in either an anti- or pro-normative direction relative to normative members. In Study 1, in-group gender-normative members were rated more positively than deviant members. The pro-norm deviant was viewed as more attractive than the anti-norm deviant. In Study 2 anti-norm in-group deviants were evaluated more negatively than anti-norm out-group deviants even though both held identical attitudes. In both studies, despite objective equivalence, pro-norm deviance was perceived as less "atypical" than anti-norm deviance. Judgments and reactions to deviance depend on group membership and the direction of deviance, not just its magnitude. Evaluations of deviants are also related to perceivers' identification with their own group. These findings are consistent with our model of subjective group dynamics.
Evolutionary Implications of Horizontal Gene Transfer (2012)

The flow of genes between different species represents a form of genetic variation whose implications have not been fully appreciated. Here I examine some key findings on the extent of horizontal gene transfer (HGT) revealed by comparative genome analysis and their theoretical implications. In theoretical terms, HGT affects ideas pertaining to the tree of life, the notion of a last universal common ancestor, and the biological unities, as well as the rules of taxonomic nomenclature. This review discusses the emergence of the eukaryotic cell and the occurrence of HGT among metazoan phyla involving both transposable elements and structural genes for normal housekeeping functions. I also discuss the bacterial pangenome, which provides an important case study on the permeability of species boundaries. An interesting observation about bdelloid rotifers and their reversion to asexual reproduction as it pertains to HGT is included.
Acute Helicobacter pylori infection: clinical features, local and systemic immune response, gastric mucosal histology, and gastric juice ascorbic acid concentrations. (1991)

The symptomatology of a case of acute infection with Helicobacter pylori is described, together with the accompanying changes in gastric mucosal histology, local and systemic humoral immune response, and gastric ascorbic acid concentration. The patient was an endoscopist, previously negative for the carbon-14 urea breath test, who had a week of epigastric pain and then became asymptomatic. H pylori was detected by culture of antral biopsy specimens and was still present after 74 days. Five days after infection the histological findings showed acute neutrophilic gastritis; by day 74 changes of chronic gastritis were evident. The patient seroconverted by IgG enzyme linked immunosorbent assay by day 74, but a mucosal IgM and IgA response was evident as early as day 14. Infection was accompanied by a transient hypochlorhydria but a sustained fall in gastric juice ascorbic acid concentration.
Rationale and plans for developing a non-replicating, metabolically active, radiation-attenuated<i>Plasmodium falciparum</i>sporozoite vaccine (2003)

Annually, malaria causes >300 million clinical cases and 1 million deaths, is responsible for the loss of >1% of gross domestic product (GDP) in Africa and is a serious concern for travelers. An effective vaccine could have a dramatic impact on the disease. For 20 years, scientists have tried to develop modern, recombinant 'subunit' malaria vaccines. This has been difficult. In fact, there is only one recombinant protein vaccine on the market for any disease, and no vaccines based on synthetic peptides, recombinant viruses, recombinant bacteria or DNA plasmids. Most vaccines are based on attenuated or inactivated whole pathogens or material derived directly from the infectious agent. It is in that context that our recent report summarizing the protection of humans with attenuated Plasmodium falciparum (Pf) sporozoites produced at four different sites over 25 years is important. In studies utilizing live mosquitoes as the vaccine delivery mechanism, there was complete protection against malaria in 93% of volunteers (13/14) and 94% of challenges (33/35). Sanaria's goal is to develop and commercialize a non-replicating, metabolically active Pf sporozoite vaccine. Three practical questions must be addressed before manufacturing for clinical trials: (1) can one administer the vaccine by a route that is clinically practical; (2) can one produce adequate quantities of sporozoites; and (3) can sporozoites be produced with the physical characteristics that meet the regulatory, potency and safety requirements of regulatory authorities? Once these questions have been answered, Sanaria will demonstrate that the vaccine protects >90% of human recipients against experimental challenge with Pf sporozoites, can be produced with an efficiency that makes it economically feasible, and protects >90% of African infants and children from infection, and has severe morbidity and mortality. By producing a vaccine for travelers, Sanaria will provide the infrastructure, regulatory foundation and funds necessary to speed licensure, manufacturing and deployment of the vaccine for the infants and children who need it most.
Fast facet edge detection in image sequences using vector quantization (1992)

An application that uses vector quantization (VQ) to speed up the process of gradient magnitude edge detection for image sequences is presented. Because image VQ and this type of edge detection operate on block-based neighborhoods, it is possible to use VQ to perform the edge detection. The image is encoded with a VQ for which the edge/no edge decision has already been made for each block. The process of edge detection becomes a simple lookup of this information. The algorithm behaves as a trainable edge detector which has the advantage of having lower computational complexity than the facet edge detector. For a VQ with an average rate of 6 bits per vector, the method requires 55% of the multiplications and 62% of the additions of the conventional facet edge detector. It also enhances the quality of the output by rejecting low-contrast, high-frequency texture edges.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Flattening and parallelizing irregular, recurrent loop nests (1995)

Irregular loop nests in which the loop bounds are determined dynamically by indexed arrays are difficult to compile into expressive parallel constructs, such as segmented scans and reductions. In this paper, we describe a suite of transformations to automatically parallelize such irregular loop nests, even in the presence of recurrences. We describe a simple, general loop flattening transformation, along with new optimizations which make it a viable compiler transformation. A robust recurrence parallelization technique is coupled to the loop flattening transformation, allowing parallelization of segmented reductions, scans, and combining-sends over arbitrary associative operators. We discuss the implementation and performance results of the transformations in a parallelizing Fortran 77 compiler for the Cray C90 supercomputer. In particular, we focus on important sparse matrix-vector multiplication kernels, for one of which we are able to automatically derive an algorithm used by one of the fastest library routines available.
Anti-Gr-1 antibody depletion fails to eliminate hepatic myeloid-derived suppressor cells in tumor-bearing mice (2012)

Recent studies show that the liver is a preferred organ for the accumulation of MDSC. In this study, we examined the effect of systemic RB6-8C5 treatment on hepatic MDSC in tumor-bearing mice. EL4 tumor-bearing mice were injected i.p. with RB6-8C5, and hepatic, splenic, and blood MDSCs were analyzed by flow cytometry. Unexpectedly, hepatic MDSC remained in the liver, although RB6-8C5 completely eliminated them from the spleen and peripheral blood 24 h after treatment. Secondary antibody staining confirmed the presence of RB6-8C5-bound MDSC in the liver of mice with s.c. tumors. Similar observations were made in two other (colon and melanoma) tumor models. Whereas RB6-8C5 injection induced cell death of hepatic MDSC, as shown by Annexin V/7-AAD staining, these cells were replaced immediately, leading to a constant, increased frequency of hepatic MDSC. Adoptively transferred MDSC migrated preferentially to the liver after RB6-8C5 treatment, suggesting that hepatic MDSCs are reconstituted rapidly after depletion. Finally, hepatic MDSC remained immunosuppressive despite RB6-8C5 injection. Our study demonstrates that RB6-8C5 is not suitable for depletion of hepatic MDSCs and analysis of their function.
The association between oxidative stress and obstructive lung impairment in patients with COPD (2007)

An oxidant/antioxidant imbalance is thought to play an important role in the pathogenesis of chronic obstructive pulmonary disease (COPD). We hypothesized that antioxidant capacity reflected by erythrocyte glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (CAT) activities, and serum levels of the lipid peroxidation product malondialdehyde (MDA), may be related to the severity of obstructive lung impairment in patients with COPD. Erythrocyte GPx, SOD and CAT activities, and serum levels of MDA were measured in 79 consecutive patients with stable COPD. Pulmonary functional tests were assessed by body plethysmography. Moderate COPD (FEV1 50-80%) was present in 23, and severe COPD (FEV1 < 50%) in 56 patients. Erythrocyte GPx activity was significantly lower, and serum MDA levels were significantly higher in patients with severe COPD compared to patients with moderate COPD (GPx: 43.1+/-1.5 vs. 47.7+/-2.9 U/gHb, p<0.05, MDA: 2.4+/-0.1 vs. 2.1+/-0.1 nmol/ml, p<0.05). Linear regression analysis revealed a significant direct relationship between FEV1 and erythrocyte GPx activity (r = 0.234, p<0.05), and a significant inverse relationship between FEV1 and serum MDA levels (r = -0.239, p<0.05). However, no differences were observed in the erythrocyte SOD and CAT activities between the two groups of patients with different severity of COPD. Findings of the present study suggest that antioxidant capacity reflected by erythrocyte GPx activity and serum levels of the lipid peroxidation product MDA are linked to the severity of COPD.
Identification of factors associated with hospital readmission and development of a predictive model. (1992)

Multiple hospital admissions, especially those related to the chronically ill, represent a particular challenge to both the acute and long-term care sectors to identify effective methods of resource management. This study analyzes the multiple admission patterns associated with a cohort of 4,219 adult medical-surgical patients discharged alive from a community teaching hospital in Michigan. The sample was divided into two groups: 3,818 patients who survived and 392 who expired during the one-year follow-up period. For the surviving subsample, the characteristics found to be directly associated with the likelihood of readmission included increased age, advanced stage of disease, greater index-episode length of stay, discharge by an internist rather than a surgeon, Medicare as expected source of payment, decreased physician age, discharge to a community setting, and increased number of prior hospital episodes. For the subsample who died, only one explanatory variable was significantly associated with an increased likelihood of readmission-discharge to a community setting (with or without home care) rather than a nursing home. The article includes illustrates of the importance of decisions regarding posthospital, long-term care services on the likelihood of rehospitalization.
Sensitivity limits for voltage control of P2Y receptor‐evoked Ca<sup>2+</sup> mobilization in the rat megakaryocyte (2003)

G-protein-coupled receptor signalling has been suggested to be voltage dependent in a number of cell types; however, the limits of sensitivity of this potentially important phenomenon are unknown. Using the non-excitable rat megakaryocyte as a model system, we now show that P2Y receptor-evoked Ca2+ mobilization is controlled by membrane voltage in a graded and bipolar manner without evidence for a discrete threshold potential. Throughout the range of potentials studied, the peak increase in intracellular Ca2+ concentration ([Ca2+]i) in response to depolarization was always larger than the maximal reduction in [Ca2+]i following an equivalent amplitude hyperpolarization. Significant [Ca2+]i increases were observed in response to small amplitude (< 5 mV, 5 s duration) or short duration (25 ms, 135 mV) depolarizations. Individual cardiac action potential waveforms were also able to repeatedly potentiate P2Y receptor-evoked Ca2+ release and the response to trains of normally paced stimuli fused to generate prolonged [Ca2+]i increases. Furthermore, elevation of the temperature to physiological levels (36 degrees C) resulted in a more sustained depolarization-evoked Ca2+ increase compared with more transient or oscillatory responses at 20-24 degrees C. The ability of signalling via a G-protein-coupled receptor to be potentiated by action potential waveforms and small amplitude depolarizations has broad implications in excitable and non-excitable tissues.