gebhart/patrepeval huggingface dataset:
P-selectin expression in a metastatic pancreatic tumor cell line (SUIT-2). (1997)

The human pancreatic tumor cell line SUIT-2 was derived from a metastatic lesion in the liver of a patient with pancreatic adenocarcinoma. SUIT-2 and clonal cell lines derived from it show spontaneous metastasis to lung and regional lymph nodes from s.c. nude mouse xenografts and were found to express P-selectin mRNA and protein. Surface expression of P-selectin protein was increased by exposure of the pancreatic tumor cells to thrombin, oxygen radicals, and trypsin, suggesting that common cellular mechanisms for regulating P-selectin surface expression exist among platelets, endothelial cells, and these pancreatic tumor cells. The finding that P-selectin is expressed by metastatic pancreatic tumor cells demonstrates that the range of cell types that express these adhesion molecules is broader than believed previously.
Direct inscription of Bragg gratings in coated fibers by an infrared femtosecond laser (2006)

Inscription of fiber Bragg gratings through the coating of a nonphotosensitized standard telecommunication fiber is demonstrated for what is believed to be the first time. Highly reflective gratings were produced by direct point-by-point writing with an infrared femtosecond laser. The length of the gratings presented ranged from 5 to 26 mm. The technique does not require a special coating, as standard coatings are transparent to infrared radiation. Inscription through the coating improves the mechanical strength of the processed segment of fiber.
The Inositol Phosphate/Diacylglycerol Pathway in MA-10 Leydig Tumor Cells (1989)

It is now well established that mouse epidermal growth factor (mEGF) modulates the hormonal responsiveness of MA-10 Leydig tumor cells but does not affect cell multiplication. The studies presented herein are the first in a series of experiments designed to characterize the intracellular signaling systems activated by mEGF and their possible roles in mediating the diverse biological actions of this growth factor in MA-10 cells. We show that (i) MA-10 cells express a hormone-sensitive inositol phosphate/diacylglycerol pathway that can be stimulated with arginine vasopressin (AVP), (ii) mEGF does not activate this pathway, and (iii) activation of this pathway with arginine vasopressin does not mimic the biological actions of mEGF. Other data presented show that lutropin/choriogonadotropin, the principal endocrine regulators of Leydig cell function, also do not stimulate the inositol phosphate/diacylglycerol pathway in MA-10 cells.
Effects of Mechanical Site Preparation on Growth of Oaks Planted on Former Agricultural Fields (2011)

Mechanical site preparation is frequently proposed to alleviate problematic soil conditions when afforesting retired agricultural fields. Without management of soil problems, any seedlings planted in these areas may exhibit poor growth and survival. While mechanical site preparation methods currently employed in hardwood afforestation are proven, there is a substantial void in research comparing subsoiling, bedding, and combination plowing treatments. A total of 4,320 bare-root Nuttall oak (Quercus texana Buckley), Shumard oak (Quercus shumardii Buckley), and swamp chestnut oak (Quercus michauxii Nutt.) seedlings were planted in February 2008 on three Mississippi sites. All sites were of comparable soils and received above average precipitation throughout the three-year duration of the study. Four site preparation treatments were replicated at each site, with 480 seedlings planted in each of nine replications, and a total of 1,440 seedlings per species planted across all sites. Mechanical treatments were installed using 3.1 m row centers, with treatments as follows: control, subsoiling, bedding, and combination plowing. Treatment effects on seedling height, groundline diameter (GLD), and survival were analyzed. Seedlings exhibited greater height in bedded and combination plowed areas (79.7 cm to 102.7 cm and 82.6 cm to 100.1 cm, respectively) compared to subsoiled or control areas (70.4 cm to 84.6 cm and 71.4 cm to 86.9 cm, respectively). Greater GLD was observed in bedded and combination plowed areas (11.9 mm to 18.4 mm and 12.2 mm to 18.3 mm, respectively) compared to subsoiled or control areas (10.2 mm to 14.6 mm and 10.5 mm to 15.6 mm, respectively). Survival was high for this study (94.%), and no differences were detected among treatments.
σ<sup>E</sup>‐dependent small RNAs of <i>Salmonella</i> respond to membrane stress by accelerating global <i>omp</i> mRNA decay (2006)

The bacterial envelope stress response (ESR) is triggered by the accumulation of misfolded outer membrane proteins (OMPs) upon envelope damage or excessive OMP synthesis, and is mediated by the alternative sigma factor, sigmaE. Activation of the GE pathway causes a rapid downregulation of major omp mRNAs, which prevents further build-up of unassembled OMPs and liberates the translocation and folding apparatus under conditions that require envelope remodelling. The factors that facilitate the rapid removal of the unusually stable omp mRNAs in the ESR were previously unknown. We report that in Salmonella the ESR relies upon two highly conserved, sigmaE-controlled small non-coding RNAs, RybB and MicA. By using a transcriptomic approach and kinetic analyses of target mRNA decay in vivo, RybB was identified as the factor that selectively accelerates the decay of multiple major omp mRNAs upon induction of the ESR, while MicA is proposed to facilitate rapid decay of the single ompA mRNA. In unstressed bacterial cells, the two oE-dependent small RNAs function within a surveillance loop to maintain envelope homeostasis and to achieve autoregulation of oE.
Intramolecular formal [4+2] cycloaddition of 3-ethoxycyclobutanones and alkenes (2010)

Intramolecular formal [4+2] cycloaddition between 3-ethoxycyclobutanones and a carbon-carbon double bond to the corresponding bicyclo[4.n.0]alkan-2-one derivatives proceeded effectively by using ethylaluminium dichloride.
Long-lasting breaches in the bladder epithelium lead to storage dysfunction with increase in bladder PGE<sub>2</sub> levels in the rat (2008)

Increase in bladder mucosal permeability can be reproduced by intravesical administration of protamine sulfate (PS); however, the influence of PS once administered into the bladder disappears within several days. We developed a chronic animal model of urothelial injury using PS. Insertion of a polyethylene catheter through the bladder dome was performed in female Wistar rats. The other end of the catheter was connected to an osmotic pump for continuous delivery of PS or vehicle for 2 wk. Urinary frequency (UF) and voided volume (VV) were measured in the metabolic cage. The fifth group of rats received a high dose of PS (10 mg/ml) for 2 wk and were followed for a further 2 wk without PS. The sixth group received a high dose of PS for 2 wk and loxoprofen (0.1 mg.kg(-1).day(-1)) for 4 wk. UF was increased, and VV was reduced in rats treated with a high dose of PS but not changed in rats treated with a vehicle or a low dose of PS (1 mg/ml). UF was further increased in the fifth group, while unchanged in the sixth group. Histological sections in rats treated with a high dose of PS demonstrated a loss of the upper layer of urothelial cells and an increased number of mast cells. PGE2 level in the bladder was significantly elevated in the fifth group. These results indicate that chronic urotherial injury leads to an increase in UF and a decrease in VV. Increased PGE2 level in the bladder is likely to be associated with long-lasting storage dysfunction.
Synthesis of Fe Nanoparticles Functionalized with Oleic Acid Synthesized by Inert Gas Condensation (2014)

In this work, we study the synthesis of monodispersed Fe nanoparticles (Fe‐NPs) in situ functionalized with oleic acid. The nanoparticles were self‐assembled by inert gas condensation (IGC) technique by using magnetron‐sputtering process. Structural characterization of Fe‐NPs was performed by transmission electron microscopy (TEM). Particle size control was carried out through the following parameters: (i) condensation zone length, (ii) magnetron power, and (iii) gas flow (Ar and He). Typically the nanoparticles generated by IGC showed diameters which ranged from ~0.7 to 20 nm. Mass spectroscopy of Fe‐NPs in the deposition system allowed the study of in situ nanoparticle formation, through a quadrupole mass filter (QMF) that one can use together with a mass filter. When the deposition system works without quadrupole mass filter, the particle diameter distribution is around +/−20%. When the quadrupole is in line, then the distribution can be reduced to around +/−2%.
The emergence of new strains of SARS-CoV-2. What does it mean for COVID-19 vaccines? (2021)

A novel strain of coronavirus, SARS-CoV-2, the causative agent of coronavirus disease (COVID-19) was first associated with severe acute respiratory disease in late 2019 and has triggered an ongoing...
In concert: artistic approaches to teacher education (2014)

"Teaching is an art, and teachers are artists. By examining the key phases in the growth of an artist—practice, technique and theory, and inspiration—we can learn how to better structure teacher education. The article follows the author’s experience and emphasizes experiential learning and structures such as apprenticeship and mentorship. It explores the philosophy of the El Sistema orchestra program in Venezuela and encourages a multi-faceted definition of artists and teachers."
Active guidance of a handheld micromanipulator using visual servoing (2009)

In microsurgery, a surgeon often deals with anatomical structures of sizes that are close to the limit of the human hand accuracy. Robotic assistants can help to push beyond the current state of practice by integrating imaging and robot-assisted tools. This paper demonstrates control of a handheld tremor reduction micromanipulator with visual servo techniques, aiding the operator by providing three behaviors: snap-to, motion-scaling, and standoff-regulation. A stereo camera setup viewing the workspace under high magnification tracks the tip of the micromanipulator and the desired target object being manipulated. Individual behaviors activate in task-specific situations when the micromanipulator tip is in the vicinity of the target. We show that the snap-to behavior can reach and maintain a position at a target with an accuracy of 17.5 ± 0.4μm Root Mean Squared Error (RMSE) distance between the tip and target. Scaling the operator's motions and preventing unwanted contact with non-target objects also provides a larger margin of safety.
Probing a protein–protein interaction by <i>in vitro</i> evolution (2006)

In this study, we used in vitro protein evolution with ribosome and phage display to optimize the affinity of a human IL-13-neutralizing antibody, a therapeutic candidate for the treatment of asthma, >150-fold to 81 pM by using affinity-driven stringency selections. Simultaneously, the antibody potency to inhibit IL-13-dependent proliferation in a cell-based functional assay increased 345-fold to an IC50 of 229 pM. The panoply of different optimized sequences resulting from complementarity-determining region-targeted mutagenesis and error-prone PCR using ribosome display was contrasted with that of complementarity-determining region-targeted mutagenesis alone using phage display. The data highlight the advantage of the ribosome-display approach in identifying beneficial mutations across the entire sequence space. A comparison of mutation hotspots from in vitro protein evolution to knockout mutations from alanine scanning demonstrated that in vitro evolution selects the most appropriate positions for improvements in potency without mutating any of the key residues within the functional paratope.
Renal Manifestations of Sarcoidosis (1981)

Sarcoidosis may involve the kidneys in several ways. Most commonly, aberrations of calcium metabolism, including hypercalcemia, hypercalciuria, and nephrocalcinosis, are responsible for the renal manifestations of sarcoidosis. Granulomatous infiltration of the renal interstitium may also produce severe derangements of renal function. Glomerulonephritis can occur with sarcoidosis, although the pathogenesis remains unclear. Besides renal insufficiency and frank renal failure, nephrotic syndrome, nephrolithiasis, hypertension, and a variety of tubular defects may complicate sarcoidosis. The sensitivity of "sarcoid nephropathy" to corticosteroids usually warrants therapeutic trial.
Estimation of thyroxin, triiodothyronine, thyrotropin, free thyroxin, and triiodothyronine uptake by use of magnetic-particle solid phases. (1985)

We evaluated solid-phase radioassays involving small, uniform magnetic particles (MAGIC, Corning Medical), obtaining data on total serum thyroxin (T4), triiodothyronine (T3), thyrotropin (TSH), free thyroxin (FT4), and T3 uptake for a total of 301 serum samples from euthyroid patients; patients receiving replacement thyroxin; patients receiving estrogen, or who were pregnant; hyperthyroid, hypothyroid, and nonthyroidally ill patients; and patients receiving salicylates, phenytoin, or heparin. We found each procedure to be technically simple and precise (between-assay CVs for T4 2.8-8.2%, T3 9-7.4%, TSH 2.9-4.5%, FT4 3.6-11%, T3 uptake 2.2-3.7%). The magnetic separations are rapid (2 to 5 min), reproducible, and complete. Each MAGIC assay produced clinically appropriate results. These assay systems offer the convenience associated with noncentrifugation assays and excellent analytical performance.
4th Microbiome R&D and Business Collaboration Forum, and Probiotics Congress, April 3–4, 2017, Amsterdam, The Netherlands (2017)

The microbial composition of the gut changes gradually with aging and is influenced by habitual diet and health status. In one cohort studied, frail elderly had a microbiota profile characterized by an increase in Bacteroidetes and a low microbiota diversity compared to healthy elderly. Paul W. O'Toole and colleagues (Cork, Ireland) developed a bacterial artificial consortium that mimics the healthy gut microbiota composition and potentially rectifies the gut microbiota of the frail elderly. More than 700 strains belonging to 90 bacterial species and one archaeon species were isolated in ore culture from the fecal samples of 7 healthy donors.
Adhesion of anaerobic microorganisms to solid surfaces and the effect of sequential attachment on adhesion characteristics (2003)

The attachment of three anaerobic microorganisms, Desulfomonile tiedjei, Syntrophomonas wolfei, and Desulfovibrio sp. strain G11, was investigated to determine if the presence of one species could influence the adhesion of another species to glass surfaces. The results indicated that the numbers and distribution of attached cells of one species could be influenced considerably by the presence of another species and the order in which the test species were exposed to the surface. D. tiedjei was found to detach readily from surfaces when it was not the primary colonizer. The attachment of Desulfovibrio G11 as the primary colonizer appeared to be stabilized by exposure to another test species. Under certain experimental conditions the test organisms formed close associations with each other on the surfaces. These findings demonstrate that the characteristics of anaerobic community biofilms can be determined by both the adhesion characteristics of the individual species and the interactions among those microorganisms.
One Hong Kong, Two Histories: 'History' and 'Chinese history' in the Hong Kong school curriculum (2002)

Hong Kong's school history curriculum is unique worldwide in that it consists of two entirely separate subjects--'History' and 'Chinese History'--which differ not only in content, but also in terms of their pedagogy and their assumptions concerning the nature of history as a discipline. The distinct subject of 'Chinese History' was first created in the 1950s, largely in response to the colonial government's desire to limit the politicisation of local schools. However, there was subsequently little interference by the government in the development of curricula for the 'two histories'. The pattern of curriculum development for history in Hong Kong over the past few decades does not support conventional theories concerning the impact of colonialism on education. On the contrary, it suggests that the relationship of colonialism to curriculum development may in Hong Kong's case be better understood in terms of a mutually convenient collaboration between the government and local educational elites. </br>[Copyright of Comparative Education is the property of Routledge. Full article may be available at the publisher's website: </br>http://dx.doi.org/10.1080/03050060120103865]
Selective inhibition of constitutive nitric oxide synthase by L-NG-nitroarginine (1993)

L-NG-Nitroarginine (NA) inhibited both the L-arginine oxidation and the L-arginine-independent NADPH oxidation reactions catalyzed by the calcium/calmodulin-dependent constitutive nitric oxide synthase (cNOS) from bovine brain. NA binding did not require calmodulin, calcium, or NADPH. The onset of inhibition was slow with a second-order association rate constant (k(on) of 4.4 x 10(4) M-1 s-1. The dissociation rate constant (k(off) was 6.5 x 10(-4) s-1. The Kd value (k(off)/k(on)) of bovine brain cNOS for NA was 15 nM. L-Arginine was a competitive inhibitor of NA binding with a Ks value of 0.8 microM. The Km for L-arginine in the cNOS reaction was 1.2 microM. The NA binding sites of cNOS were titrated with NA, which enabled a kcat of 0.7 s-1, for the oxidation of L-arginine, to be calculated. Finally, a brain cNOS-(3H)NA complex was isolated. In contrast to the potent and slow onset of NA inhibition of brain cNOS, NA inhibition of inducible mouse macrophage NOS (iNOS) was weaker (Ki = 4.4 microM) and rapidly reversible. Thus, NA was a 300-fold more potent inhibitor of bovine brain cNOS than mouse macrophage iNOS.
The clinical relevance of omega-3 fatty acids in the management of hypertriglyceridemia (2016)

Hypertriglyceridemia (triglycerides > 150 mg/dL) affects ~25 % of the United States (US) population and is associated with increased cardiovascular risk. Severe hypertriglyceridemia (≥ 500 mg/dL) is also a risk factor for pancreatitis. Three omega-3 fatty acid (OM3FA) prescription formulations are approved in the US for the treatment of adults with severe hypertriglyceridemia: (1) OM3FA ethyl esters (OM3EE), a mixture of OM3FA ethyl esters, primarily eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) (Lovaza®, Omtryg™, and generics); (2) icosapent ethyl (IPE), EPA ethyl esters (Vascepa®); and (3) omega-3 carboxylic acids (OM3CA), a mixture of OM3FAs in free fatty acid form, primarily EPA, DHA, and docosapentaenoic acid (Epanova®). At approved doses, all formulations substantially reduce triglyceride and very-low-density lipoprotein levels. DHA-containing formulations may also increase low-density lipoprotein cholesterol. However, this is not accompanied by increased non-high-density lipoprotein cholesterol, which is thought to provide a better indication of cardiovascular risk in this patient population. Proposed mechanisms of action of OM3FAs include inhibition of diacylglycerol acyltransferase, increased plasma lipoprotein lipase activity, decreased hepatic lipogenesis, and increased hepatic β-oxidation. OM3CA bioavailability (area under the plasma concentration-time curve from zero to the last measurable concentration) is up to 4-fold greater than that of OM3FA ethyl esters, and unlike ethyl esters, the absorption of OM3CA is not dependent on pancreatic lipase hydrolysis. All three formulations are well tolerated (the most common adverse events are gastrointestinal) and demonstrate a lack of drug-drug interactions with other lipid-lowering drugs, such as statins and fibrates. OM3FAs appear to be an effective treatment option for patients with severe hypertriglyceridemia.
Low frequency radar targets 3-dimensional imaging using ramp response signatures (2009)

Low frequency imaging in radar domain can have applications for stealthy or buried targets. We consider a technique using transient scattering response from a ramp waveform, which is proportional to the profile function of the target, i.e. its transverse cross-sectional area along the line-of-sight, and consequently, gives information about target size, orientation and geometrical shape. Usual 3-dimensional image generation from ramp responses uses approximate limiting surfaces and is limited to single convex objects. We propose here a new algorithm to reconstruct the global shape of any perfectly conducting target from its ramp response signatures.
Analysis of the association of chronic spontaneous urticaria with interlekin-4, -10, transforming growth factor-β1, interferon-γ, interleukin-17A and -23 by autologous serum skin test (2017)

These results, when considered together with current reports in the literature, indicate that immune dysregulation occurs in the pathogenesis of CSU, causing cytokine imbalance.
SELOVS: Brain MRSI localization based on highly selective <i>T</i><sub>1</sub>‐ and <i>B</i><sub>1</sub>‐insensitive outer‐volume suppression at 3T (2007)

In vivo, high-field MR spectroscopic imaging (MRSI) profits from signal-to-noise ratio (SNR) gain and increased spectral resolution. However, bandwidth limitations of slice-selective excitation and refocusing pulses lead to strong chemical-shift displacement at high field strength when using conventional MRSI localization based on PRESS. Consequential metabolic information, particularly of border regions such as cortical brain tissue, is distorted. In addition, lipid contamination remains a major confound. To address these problems it is proposed to abandon PRESS selection and rely on a novel scheme of highly selective T(1)- and B(1)-insensitive outer-volume suppression in combination with slice-selective spin-echo acquisition for brain MRSI. Multiple cycles of overlapping suppression slabs are applied with flip angles optimized to account for tissue-dependent T(1) relaxation times and band crossings. Broadband frequency modulated saturation pulses with polynomial phase-response are utilized in order to minimize chemical-shift displacement. Efficacy of the outer-volume suppression sequence was simulated and evaluated in vitro and in vivo. Brain MRSI localization at 3T was significantly improved and reliable suppression of short-range lipid contamination enabled, leading to substantial enhancement of spectral quality, particularly in cortical tissue. Hence, the new method holds potential to expand the applicability of high-field MRSI to the entire brain.
Coupled Marchenko equations for electromagnetic Green’s function retrieval and imaging (2013)

Recently a new theory has been developed to retrieve a wavefield generated by a source on the surface and recorded at a point in the subsurface without the need for a receiver at that subsurface location. The scheme is presented for three-dimensional wavefields. It decomposes the electromagnetic field in up- and downgoing electric fields and in TE- and TM-modes. Each mode can be treated separately to construct the Green’s function. We derive two coupled Marchenko equations from which the up- and downgoing Green’s functions can be obtained. These two directional Green’s functions have applications in true-amplitude subsurface imaging without effects from internal multiple reflections.