gebhart/patrepeval huggingface dataset:
Electrosensitization assists cell ablation by nanosecond pulsed electric field in 3D cultures (2016)

Previous studies reported a delayed increase of sensitivity to electroporation (termed "electrosensitization") in mammalian cells that had been subjected to electroporation. Electrosensitization facilitated membrane permeabilization and reduced survival in cell suspensions when the electric pulse treatments were split in fractions. The present study was aimed to visualize the effect of sensitization and establish its utility for cell ablation. We used KLN 205 squamous carcinoma cells embedded in an agarose gel and cell spheroids in Matrigel. A local ablation was created by a train of 200 to 600 of 300-ns pulses (50 Hz, 300-600 V) delivered by a two-needle probe with 1-mm inter-electrode distance. In order to facilitate ablation by engaging electrosensitization, the train was split in two identical fractions applied with a 2- to 480-s interval. At 400-600 V (2.9-4.3 kV/cm), the split-dose treatments increased the ablation volume and cell death up to 2-3-fold compared to single-train treatments. Under the conditions tested, the maximum enhancement of ablation was achieved when two fractions were separated by 100 s. The results suggest that engaging electrosensitization may assist in vivo cancer ablation by reducing the voltage or number of pulses required, or by enabling larger inter-electrode distances without losing the ablation efficiency.
Contour reconstruction in 3-D X-ray CT (1993)

The authors derives an algorithm for reconstructing contours of an object from 3D cone beam X-ray data. By reducing the amount of the searched-for information, contours, or density jumps instead of the densities themselves, the authors are able to develop fast algorithms for data incomplete with respect to both the movement of the X-ray source and the detector reading. The method is related to local or Lambda tomography. Numerical simulations show the efficiency of the algorithm.
Analysis of the Involvement of an Inducible <i>Arabidopsis</i> RNA-Dependent RNA Polymerase in Antiviral Defense (2003)

RNA-dependent RNA polymerases (RdRPs) have been implicated in posttranscriptional gene silencing (PTGS) and antiviral defense. An Arabidopsis RdRP (SDE1/SGS2) has been previously shown to be required for transgene-induced PTGS but has no general role in antiviral defense. On the other hand, we have recently shown that transgenic tobacco deficient in an inducible RdRP (NtRdRP1) activity became more susceptible to both Tobacco mosaic virus and Potato virus X. Thus, different RdRPs may have distinct roles in closely related PTGS and antiviral defense. In the present study, we analyzed roles of a newly identified Arabidopsis RdRP gene (AtRdRP1) in plant antiviral defense. AtRdRP1 encodes an RdRP closely related structurally to NtRdRP1 and is also induced by salicylic acid treatment and virus infection. A T-DNA insertion mutant for AtRdRP1 has been isolated and analyzed for possible alterations in response to viral infection. When infected by a tobamovirus and a tobravirus, the knockout mutant accumulated higher and more persistent levels of viral RNAs in both the lower, inoculated and in upper, systemically infected leaves than did wild-type plants. These results suggest that the inducible AtRdRP1 is the Arabidopsis ortholog of NtRdRP1 and plays a role in antiviral defense. Examination of short viral RNAs and silencing studies using a viral vector harboring an endogenous plant gene suggest that, while not required for virus-induced PTGS, AtRdRP1 can apparently promote turnover of viral RNAs in infected plants.
Cerebrospinal Fluid and Neuroimaging Biomarker Abnormalities Suggest Early Neurological Injury in a Subset of Individuals During Primary HIV Infection (2013)

Elevated neurofilament light chain and its correlation with MRS-based metabolites suggest early neuronal injury in a subset of participants with primary HIV infection through mechanisms involving central nervous system inflammation.
Superhydrophobic Porous Surfaces: Dissolved Oxygen Sensing (2015)

Porous polymer films are necessary for dissolved gas sensor applications that combine high sensitivity with selectivity. This report describes a greatly enhanced dissolved oxygen sensor system consisting of amphiphilic acrylamide-based polymers: poly(N-(1H, 1H-pentadecafluorooctyl)-methacrylamide) (pC7F15MAA) and poly(N-dodecylacrylamide-co-5- [4-(2-methacryloyloxyethoxy-carbonyl)phenyl]-10,15,20-triphenylporphinato platinum(II)) (p(DDA/PtTPP)). The nanoparticle formation capability ensures both superhydrophobicity with a water contact angle greater than 160° and gas permeability so that molecular oxygen enters the film from water. The film was prepared by casting a mixed solution of pC7F15MAA and p(DDA/PtTPP) with AK-225 and acetic acid onto a solid substrate. The film has a porous structure comprising nanoparticle assemblies with diameters of several hundred nanometers. The film shows exceptional performance as the oxygen sensitivity reaches 126: the intensity ratio at two oxygen concentrations (I0/I40) respectively corresponding to dissolved oxygen concentration 0 and 40 (mg L(-1)). Understanding and controlling porous nanostructures are expected to provide opportunities for making selective penetration/separation of molecules occurring at the superhydrophobic surface.
Advanced Web Metrics with Google Analytics (2008)

Get the latest information about using the #1 web analytics tool from this fully updated guide Google Analytics is the free tool used by millions of web site owners to assess the effectiveness of their efforts. Its revised interface and new features will offer even more ways to increase the value of your web site, and this book will teach you how to use each one to best advantage. Featuring new content based on reader and client requests, the book helps you implement new methods and concepts, track social and mobile visitors, use the new multichannel funnel reporting features, understand which
TRPC1 and TRPC6 Channels Cooperate with TRPV4 to Mediate Mechanical Hyperalgesia and Nociceptor Sensitization (2009)

The transient receptor potential vanilloid 4 (TRPV4) contributes to mechanical hyperalgesia of diverse etiologies, presumably as part of a mechanoreceptor signaling complex (Alessandri-Haber et al., 2008). To investigate the hypothesis that a functional interaction between TRPV4 and stretch-activated ion channels (SACs) is involved in this mechanical transduction mechanism, we used a selective SACs inhibitor, GsMTx-4. Intradermal injection of GsMTx-4 in the rat hindpaw reversed the mechanical hyperalgesia induced by intradermal injection of inflammatory mediators. In vivo single fiber recordings showed that GsMTx-4 reversed inflammatory mediator-induced decrease in mechanical threshold in half of sensitized C-fibers. Furthermore, GsMTx-4 reduced hyperalgesia to both mechanical and hypotonic stimuli in different models of inflammatory and neuropathic pain, although it had no effect on baseline mechanical nociceptive thresholds. TRPC1 and TRPC6, two GsMTx-4-sensitive SACs, are expressed in dorsal root ganglion (DRG) neurons. Single-cell reverse transcription-PCR showed that messenger RNAs for TRPV4, TRPC1, and TRPC6 are frequently coexpressed in DRG neurons. Spinal intrathecal administration of oligodeoxynucleotides antisense to TRPC1 and TRPC6, like that to TRPV4, reversed the hyperalgesia to mechanical and hypotonic stimuli induced by inflammatory mediators without affecting baseline mechanical nociceptive threshold. However, antisense to TRPC6, but not to TRPC1, reversed the mechanical hyperalgesia induced by a thermal injury or the TRPV4-selective agonist 4alpha-PDD (4 alpha-phorbol 12,13-didecanoate). We conclude that TRPC1 and TRPC6 channels cooperate with TRPV4 channels to mediate mechanical hyperalgesia and primary afferent nociceptor sensitization, although they may have distinctive roles.
The five D's of botulinum toxin: Doses, dilution, diffusion, duration and dogma (2008)

The purpose of this review is to update cosmetic dermatologists and surgeons on the latest information about botulinum toxin injections for the treatment of the face and neck and to provide a practical guide to effective and safe technique. We review indications, recommended doses and dilutions, storage recommendations and injection techniques.
Experimental study on channel crosstalk due to fiber four-wave mixing around the zero-dispersion wavelength (1994)

Channel crosstalk due to fiber four-wave mixing (FWM) in multichannel systems operated around the zero-dispersion wavelength is experimentally studied. After determining the wavelength at which FWM light is most efficiently generated, the FWM efficiency is measured for possible frequency combinations which generate FWM light at that wavelength. Using these data, FWM crosstalk in multiwavelength systems is evaluated. The results show that actual crosstalk, is less than the value estimated by the theoretical model assuming the uniformly distributed chromatic dispersion for 80-km-long fibers. It is concluded that the theoretical model can be applied to system design dealing with the worst condition.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Time-Resolved 3-Dimensional Velocity Mapping in the Thoracic Aorta (2004)

Time-resolved 3D velocity mapping was successfully applied in a study of 10 healthy volunteers and 4 patients with documented aortic pathologic findings and has proven to be a reliable tool for analysis and visualization of normal characteristic as well as pathologic flow features within the entire thoracic aorta.
Design of a P-wave seismic vibrator with advanced performance (2008)

ABSTRACT For optimal seismic imaging, the vibroseis method requires the vibrator to generate synchronous, repeatable sweeps over a broad frequency range and output the ground-force energy with minimum harmonic distortion. This requires re-evaluating each element of the vibrator system to ensure that it contributes to the success of the method. Key factors that cause the vibrator to suffer from severe harmonic distortion are fluctuations in the hydraulic power supply pressure, flexing of the baseplate, coupling or loading between the baseplate and the ground, nonlinear servo-valve flow-pressure characteristics, and servo-valve characteristics near null. This paper examines these factors and describes the design of a new P-wave vibrator by ION (previously I-O) to improve seismic resolution. Experimental results demonstrate that the newly designed vibrator dramatically reduces harmonic distortion in the ground-force signal under various coupling conditions, particularly on hard and uneven ground. With the high-frequency controller and Pelton DR valve, a broad-band sweep frequency is achieved. Cavitations in the supply pressure, a long-standing problem in vibrator mechanics, are almost completely eliminated.
Spatial Distribution of QT Intervals: An Alternative Approach to QT Dispersion (1996)

QT dispersion (QTd) describes the heterogeneity of ventricular repolarization on the basis of the temporal range of QT intervals as measured in the 12-lead ECG. We examined the spatial distribution of QTd using multichannel magnetocardiograms (MCGs), which noninvasively register changes in magnetic field strength at 37 sites over the heart. As in ECG, the MCG signal in each channel may be used to measure QT interval. By calculating QT deviation from QTmin at each site, one can reconstruct the spatial distribution of QTd. Analysis of spatial QTd in ten healthy subjects and ten patients after acute myocardial infarction (MI) showed clear differences in spatial distribution. The healthy subjects generally displayed shorter QT intervals along a line corresponding to the approximate position of the septum with longer intervals in plateaus in the upper right and lower left. Spatial QTd of the post-MI patients deviated from this pattern, often displaying a sharp rise in QT duration over specific areas, which could be related to functional and morphological disturbances. The quantification of local irregularities as well as the overall pattern on the basis of a smoothness index allowed better discrimination between healthy subjects and post-MI patients than QTd. Distribution patterns of QTd which reflect local repolarization alterations may thus represent a more differentiated marker for pathology and risk.
Chloroalanyl antibiotic peptides: antagonism of their antimicrobial effects by L-alanine and L-alanyl peptides in gram-negative bacteria (1986)

A large number of structurally diverse di- and tripeptides containing the alanine racemase inactivator beta-chloro-L-alanine (beta-Cl-LAla) have been synthesized, and their antibacterial properties in vitro have been evaluated. The dipeptides 1, 3-6, and 8-17 and the tripeptide 20 are all broad-spectrum antibacterial agents with considerable potency against both Gram-positive and Gram-negative species, but none of these peptides improves dramatically on the antibiotic efficacy of the previously described beta-Cl-LAla-beta-Cl-LAla, 9 (Cheung, K. S.; Wasserman, S. A.; Dudek, E.; Lerner, S. A.; Johnston, M. J. Med. Chem. 1983, 26, 1733). Gram-negative microorganisms, such as Escherichia coli, Hemophilus influenzae, Shigella flexneri, and Enterobacter species are consistently resistant to any haloalanyl peptide containing an alanyl residue, such as the dipeptide LAla-beta-Cl-LAla (2) and the tripeptides LMet-LAla-beta-Cl-LAla (7), LAla-LAla-beta-Cl-LAla (18), and LVal-LAla-beta-Cl-LAla (19). Correspondingly, these same organisms are protected from the bactericidal effects of 9 by supplementation of the growth medium with LAla or LAla-LAla. Escherichia coli JSR-O exposed to 9, but protected from lysis by sucrose stabilization, has only about 10% the normal level of intracellular alanine racemase activity. But when these cells are cultured in the presence of 9 with LAla supplementation, or in the presence of 2 with no supplementation, the alanine racemase levels are only about 20-30% below control values. These findings suggest that the resistance of Gram-negative species to chloroalanyl peptides containing alanyl units arises from the ability of LAla to protect the targeted racemase from inactivation by beta-Cl-LAla in vivo, an event which otherwise leads to cell death and lysis. Inactivation of alanine racemase in Gram-positive organisms appears not to be the cellular event that confers sensitivity of these species to a haloalanyl peptide.
Demonstration of HCCI Using a Single Cylinder Four-stroke SI Engine with Modified Valve Timing (2000)

<div class="htmlview paragraph">A standard port fuel injected, unthrottled single cylinder four-stroke SI engine, with a compression ratio of 10.3:1, and using standard gasoline fuel, has been adapted to operate in the homogeneous charge compression ignition (HCCI) mode, by modifying the valve timing. It has been found that over a speed range of between 1300 and 2000 rpm, and lambda values of between 0.95 and 1.1, stable operation is achieved without spark ignition. The internal EGR rate was estimated to be about 60%, and emissions of NO<sub>X</sub> were typically 0.25 g/kWh.</div> <div class="htmlview paragraph">Practical implementation of this HCCI concept will require variable valve timing, which will also enable reversion to standard SI operation for maximum power.</div>
Development of a novel rationally designed antibiotic to inhibit a nontraditional bacterial target (2017)

The search for new nontraditional targets is a high priority in antibiotic design today. Bacterial membrane energetics based on sodium ion circulation offers potential alternative targets. The present work identifies the Na<sup>+</sup>-translocating NADH:ubiquinone oxidoreductase (Na<sup>+</sup>-NQR), a key respiratory enzyme in many microbial pathogens, as indispensible for the Chlamydia trachomatis infectious process. Infection by Chlamydia trachomatis significantly increased first H<sup>+</sup> and then Na<sup>+</sup> levels within the host mammalian cell. A newly designed furanone Na<sup>+</sup>-NQR inhibitor, PEG-2S, blocked the changes in both H<sup>+</sup> and Na<sup>+</sup> levels induced by Chlamydia trachomatis infection. It also inhibited intracellular proliferation of Chlamydia trachomatis with a half-minimal inhibitory concentration in the submicromolar range but did not affect the viability of mammalian cells or bacterial species representing benign intestinal microflora. At low nanomolar concentrations (IC<sub>50</sub> value = 1.76 nmol/L), PEG-2S inhibited the Na<sup>+</sup>-NQR activity in sub-bacterial membrane vesicles isolated from Vibrio cholerae. Taken together, these results show, for the first time, that Na<sup>+</sup>-NQR is critical for the bacterial infectious process and is susceptible to a precisely targeted bactericidal compound in situ. The obtained data have immediate relevance for many different diseases caused by pathogenic bacteria that rely on Na<sup>+</sup>-NQR activity for growth, including sexually transmitted, pulmonary, oral, gum, and ocular infections.
Electrostatic Effects in Proteins (1985)

Many bacterial clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated (Cas) systems employ the dual RNA–guided DNA endonuclease Cas9 to defend against invading phages and conjugative plasmids by introducing site-specific ...Read More
Species Differentiation of a Diverse Suite of <i>Bacillus</i> Spores by Mass Spectrometry-Based Protein Profiling (2004)

In this study, we demonstrate the versatility of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOFMS) protein profiling for the species differentiation of a diverse suite of Bacillus spores. MALDI-TOFMS protein profiles of 11 different strains of Bacillus spores, encompassing nine different species, were evaluated. Bacillus species selected for MALDI-TOFMS analysis represented the spore-forming bacterial diversity of typical class 100K clean room spacecraft assembly facilities. A one-step sample treatment and MALDI-TOFMS preparation were used to minimize the sample preparation time. A library of MALDI-TOFMS spectra was created from these nine Bacillus species, the most diverse protein profiling study of the genus reported to date. Linear correlation analysis was used to successfully differentiate the MALDI-TOFMS protein profiles from all strains evaluated in this study. The MALDI-TOFMS protein profiles were compared with 16S rDNA sequences for their bacterial systematics and molecular phylogenetic affiliations. The MALDI-TOFMS profiles were found to be complementary to the 16S rDNA analysis. Proteomic studies of Bacillus subtilis 168 were pursued to identify proteins represented by the biomarker peaks in the MALDI-TOFMS spectrum. Four small, acid-soluble proteins (A, B, C, and D), one DNA binding protein, hypothetical protein ymf J, and four proteins associated with the spore coat and spore coat formation (coat JB, coat F, coat T, and spoIVA) were identified. The ability to visualize higher-molecular-mass coat proteins (10 to 25 kDa) as well as smaller proteins (<10 kDa) with MALDI-TOFMS profiling is critical for the complete and effective species differentiation of the Bacillus genus.
Remote microscope for inspection of integrated circuits (1995)

Thesis (M.S.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 1995.
Fast Mosaic of High-Resolution Multiple-View Images of Surfaces from Architectural and Heritage Structures (2007)

This paper presents an automatic and fast method for creating seamless mosaics from images captured by a special aerial imaging system when imaging architectural and heritage structures. The whole scheme was made following three principles: speediness, robustness and the adaptability to the aerial image acquisition system in our project. We first extract keypoints and match them using the newest SURF algorithm, which can generate large sets of corresponding points fast and robustly. Then, a simple least-squares algorithm can estimate the projective transform parameters. In addition, to increase the robustness, lens distortion correction and global optimization strategy are also adopted. Finally, the aligned set of registered images is processed by an image fusion technique to produce effectively seamless composite images.
Early history of cardiac pacing and defibrillation. (2002)

The Electricity and the Heart website1 is intended to facilitate the collection, cataloging and presentation of historical information about technical and scientific advances in cardiac devices. Over the course of the past century as the fields of cardiac pacing and electrophysiology have evolved, the technological devices used by physicians and researchers has been a fascinating and rapidly changing portion of the history of the fields. NASPE's Oral History Project houses hundreds of devices collected over the years that illustrate the evolution from crude and simple machines to the sophisticated and advanced technological wonders that are used in the field today. The photos and descriptions of many of these devices show just how far we have come in the advancement of treatment and patient care.
Uptake of water from soils by plant roots (2007)

Uptake of water by plant roots can be considered at two different Darcian scales, referred to as the mesoscopic and macroscopic scales. At the mesoscopic scale, uptake of water is represented by a flux at the soil–root interface, while at the macroscopic scale it is represented by a sink term in the volumetric mass balance. At the mesoscopic scale, uptake of water by individual plant roots can be described by a diffusion equation, describing the flow of water from soil to plant root, and appropriate initial and boundary conditions. The model involves at least two characteristic lengths describing the root–soil geometry and two characteristic times, one describing the capillary flow of water from soil to plant roots and another the ratio of supply of water in the soil and uptake by plant roots. Generally, at a certain critical time, uptake will switch from demand-driven to supply-dependent. In this paper, the solutions of some of the resulting mesoscopic linear and nonlinear problems are reviewed. The resulting expressions for the evolution of the average water content can be used as a basis for upscaling from the mesoscopic to the macroscopic scale. It will be seen that demand-driven and supply-dependent uptake also emerge at the macroscopic scale. Information about root systems needed to operationalize macroscopic models will be reviewed briefly.
Brownian aggregation rate of colloid particles with several active sites (2014)

We theoretically analyze the aggregation kinetics of colloid particles with several active sites. Such particles (so-called "patchy particles") are well known as chemically anisotropic reactants, but the corresponding rate constant of their aggregation has not yet been established in a convenient analytical form. Using kinematic approximation for the diffusion problem, we derived an analytical formula for the diffusion-controlled reaction rate constant between two colloid particles (or clusters) with several small active sites under the following assumptions: the relative translational motion is Brownian diffusion, and the isotropic stochastic reorientation of each particle is Markovian and arbitrarily correlated. This formula was shown to produce accurate results in comparison with more sophisticated approaches. Also, to account for the case of a low number of active sites per particle we used Monte Carlo stochastic algorithm based on Gillespie method. Simulations showed that such discrete model is required when this number is less than 10. Finally, we applied the developed approach to the simulation of immunoagglutination, assuming that the formed clusters have fractal structure.