gebhart/patrepeval huggingface dataset:
Development of a Strain Transferring Sensor Housing for a Lumbar Spinal Fusion Detection System (2006)

Lumbar arthrodesis or spinal fusion is usually performed to relieve back pain, and regain functionality from degenerative disc disease, trauma, etc. Fusion is determined from radiographic images (X-ray) or computed tomography scans, yet these inspection procedures are subjective methods of review. As a result, exploratory surgery is performed if the presence of fusion cannot be confirmed. Therefore, a need exists to provide objective data to determine the presence of fusion that could avoid the cost, pain, and risk of exploratory surgery. One method to achieve this objective is to observe bending strain from spinal rods implanted during surgery. A system has been developed that will attach to the spinal instrumentation rods, transmit strain information wirelessly, and without the use of batteries. Major components of the system include a strain transferring sensor housing, a microelectromechanical (MEMS)-based strain sensor, telemetry circuitry, and antennae. Only discussed herein are the design, testing, and results of the housing without a cover and its ability to transfer strain from the rod to an internal surface where a foil strain gage is attached to characterize strain transfer efficiency. Strain gauges rather than the MEMS sensor were employed for housing characterization due cost and limited availability. Design constraints for the housing are long-term implantation, small size, greater than 95% transfer of bending strain from the spinal rods to the internal strain sensor, and ease of installation. ABAQUS finite element modeling software was employed to develop a working model that was fabricated using polyetheretherkeytone. The housing underwent cycle testing in a material testing system to simulate long-term implantation along with static testing to determine if creep was present. Both series of tests showed that the housing’s response did not degrade over a period of time and there was no indication of creep. The experimental results also validated the results of the ABAQUS finite element model.
Recording characteristics of magneto-optical media with auxiliary magnetic layer. (1991)

The purpose of this study is to investigate the leakage field from an auxiliary layer to a recording layer in an exchange-coupled multilayered magnetooptical medium. In order to estimate the leakage field, test media, in which an auxiliary layer was formed on a recording layer through a non-magnetic layer, were prepared. The leakage field was determined as the difference in the threshold external fields between two different initial magnetization directions in the recording test. The experimental result was in good agreement with the numerical calculation, in which a single domain nucleation was assumed. The leakage field was found to be proportional to the change in magnetization at the heated spot. It was also found that a leakage field of about 200 Oe was generated from the auxiliary layer with a magnetization change of 150 emu/cc.
Room temperature stable helical blue phase enabled by a photo-polymerizable bent-shaped material (2016)

A designed polymerizable achiral bent-shaped material was synthesized and mixed with a chiral nematic liquid crystal to form a stable and hysteresis-free blue phase.
Synthesis and antimicrobial evaluation of acyl derivatives of 16-membered macrolide antibiotics related to tylosin. (1986)

A large number and wide variety of acyl derivatives of the tylosin-related macrolides 23-demycinosyltylosin (DMT), 23-demycinosyloxytylosin (DMOT) and 5-O-mycaminosyltylonolide (OMT) were synthesized and evaluated. This encompassed conversion of the hydroxyl groups at 2',4' and 23 of the appropriate macrolides to the corresponding esters, in which a variety of different substitution patterns were examined. A wide range of acyl substituents was investigated, particularly for 23-O-acyl derivatives of OMT, since these were substantially more active in vitro than OMT itself. However, the acyl derivatives which were prepared demonstrated no substantial improvement in oral efficacy or bioavailability over the parent macrolides.
SC‐GRAPPA: Self‐constraint noniterative GRAPPA reconstruction with closed‐form solution (2012)

SC-GRAPPA offers improved pMRI reconstruction, and is expected to benefit clinical imaging applications in the future.
On-chip determination of spermatozoa concentration using electrical impedance measurements (2010)

In this article we describe the development of a microfluidic chip to determine the concentration of spermatozoa in semen, which is a main quality parameter for the fertility of a man. A microfluidic glass-glass chip is used, consisting of a microchannel with a planar electrode pair that allows the detection of spermatozoa passing the electrodes using electrical impedance measurements. Cells other than spermatozoa in semen also cause a change in impedance when passing the electrodes, interfering with the spermatozoa count. We demonstrate that the change in electrical impedance is related to the size of cells passing the electrodes, allowing to distinguish between spermatozoa and HL-60 cells suspended in washing medium. In the same way we are able to distinguish between polystyrene beads and spermatozoa. Thus, by adding a known concentration of polystyrene beads to a boar semen sample, the spermatozoa concentrations of seven mixtures are measured and show a good correlation with the actual concentration (R(2)-value = 0.97). To our knowledge this is the first time that the concentration of spermatozoa has been determined on chip using electrical impedance measurements without a need to know the actual flow speed. The proposed method to determine the concentration can be easily applied to other cells. The described on-chip determination of the spermatozoa concentration is a first step towards a microfluidic system for a complete quality analysis of semen.
Bioconversion of m-hydroxybenzoate to 2,3-dihydroxybenzoate by mutants of Pseudomonas testosteroni (1980)

Mutans of Pseudomonas testosteroni were isolated for their inability to grow on m-hydroxybenzoate as sole carbon source. These mutants hydroxylated m-hydroxybenzoate for form 2,3-dihydroxybenzoate in high yeilds. The bioconversion described in this report represents the first reported example of 3-hydroxybenzoate 2-hydroxylase activity.
Carbon Nanotubes: Ultrabreathable and Protective Membranes with Sub‐5 nm Carbon Nanotube Pores (Adv. Mater. 28/2016) (2016)

A flexible membrane with sub-5 nm single-walled carbon nanotube (SWNT) pores is developed by F. Fornasiero and co-workers, as described on page 5871, for application as a key component of protective, yet breathable fabrics. The SWNTs are shown to enable exceptionally fast transport of water vapor under a concentration driving force. Thus, membranes having SWNTs as moisture-conductive pores feature outstanding breathability and provide a high degree of protection from biological threats by size exclusion.
The sialyltransferase ST3GAL6 influences homing and survival in multiple myeloma (2014)

Glycosylation is a stepwise procedure of covalent attachment of oligosaccharide chains to proteins or lipids, and alterations in this process, especially increased sialylation, have been associated with malignant transformation and metastasis. The role of altered sialylation in multiple myeloma (MM) cell trafficking has not been previously investigated. In the present study we identified high expression of β-galactoside α-2,3-sialyltransferase, ST3GAL6, in MM cell lines and patients. This gene plays a key role in selectin ligand synthesis in humans through the generation of functional sialyl Lewis X. In MRC IX patients, high expression of this gene is associated with inferior overall survival. In this study we demonstrate that knockdown of ST3GAL6 results in a significant reduction in levels of α-2,3-linked sialic acid on the surface of MM cells with an associated significant reduction in adhesion to MM bone marrow stromal cells and fibronectin along with reduced transendothelial migration in vitro. In support of our in vitro findings, we demonstrate significantly reduced homing and engraftment of ST3GAL6 knockdown MM cells to the bone marrow niche in vivo, along with decreased tumor burden and prolonged survival. This study points to the importance of altered glycosylation, particularly sialylation, in MM cell adhesion and migration.
Structure-based inhibitors halt prion-like seeding by Alzheimer’s disease–and tauopathy–derived brain tissue samples (2019)

In Alzheimer's disease (AD) and tauopathies, tau aggregation accompanies progressive neurodegeneration. Aggregated tau appears to spread between adjacent neurons and adjacent brain regions by prion-like seeding. Hence, inhibitors of this seeding offer a possible route to managing tauopathies. Here, we report the 1.0 Å resolution micro-electron diffraction structure of an aggregation-prone segment of tau with the sequence SVQIVY, present in the cores of patient-derived fibrils from AD and tauopathies. This structure illuminates how distinct interfaces of the parent segment, containing the sequence VQIVYK, foster the formation of distinct structures. Peptide-based fibril-capping inhibitors designed to target the two VQIVYK interfaces blocked proteopathic seeding by patient-derived fibrils. These VQIVYK inhibitors add to a panel of tau-capping inhibitors that targets specific polymorphs of recombinant and patient-derived tau fibrils. Inhibition of seeding initiated by brain tissue extracts differed among donors with different tauopathies, suggesting that particular fibril polymorphs of tau are associated with certain tauopathies. Donors with progressive supranuclear palsy exhibited more variation in inhibitor sensitivity, suggesting that fibrils from these donors were more polymorphic and potentially vary within individual donor brains. Our results suggest that a subset of inhibitors from our panel could be specific for particular disease-associated polymorphs, whereas inhibitors that blocked seeding by extracts from all of the tauopathies tested could be used to broadly inhibit seeding by multiple disease-specific tau polymorphs. Moreover, we show that tau-capping inhibitors can be transiently expressed in HEK293 tau biosensor cells, indicating that nucleic acid-based vectors can be used for inhibitor delivery.
Tree Notification to Improve Multicast Fast Reroute (2014)

This draft proposes dataplane triggered Tree Notifications to support
multicast fast reroute for PIM and mLDP. These Tree Notifications are
initiated by a node detecting the failure to a Repair Node downstream.
A Repair Node is a node that has a pre-built backup path that can
circumvent the failure. Using this mechanism, a Repair Node has the
ability to learn about non-local failures quickly without having to
wait for the IGP to convergence. This draft also covers an optional
method to avoid bandwidth usage on the pre-built backup path.
Development of Looper Shape Meter and Verification Test Results in Hot Rolling (2014)

The harder and thinner strips, such as high strength steel and so on, have been needed to be rolled. When the rolled strip becomes thinner and harder, the unstable state rolling and tail end pinching might occur and so the strip shape measurement during rolling is strongly required under the condition that tension is applied to the strip. Visual-type shape meter, which has been already used, could not measure the strip shape when tension is applied to the strip. Looper Shape Meter, hereinafter referred as LSM, has been developed which could measure the strip shape under the tension. LSM is installed between mill stands and consists of 7 segment rolls along the width direction and a segment roll has two torque meters. LSM could accurately measure the tension distribution along the width direction without hysteresis and is protected with purge air and internal cooling system against severe environment in hot rolling. In order to verify the performance of LSM, the verification test was carried out in laboratory and production line. As a result, enough durability and good results of measured strip shape are obtained. LSM is easily replaced with existing conventional looper and it is expected that LSM contributes to the stable rolling and improve the productivity of rolling.
Speed Sensorless Estimation of AC Induction Motors Using the Fast Orthogonal Search Algorithm (2006)

This paper presents a method of estimating the speed of an induction motor using a measurement of the stator current. Speed-induced current harmonics are identified in the stator current using the fast orthogonal search (FOS) algorithm. The frequencies of these estimated harmonics are in turn used to estimate the speed of the motor given the number of rotor slots in the motor. Several optimizations of the FOS algorithm are presented to allow for real-time performance on an embedded digital signal processor. Experimental results of speed estimates on a 1/4 horsepower motor are presented to verify this approach.
Iron control of erythroid development by a novel aconitase-associated regulatory pathway (2010)

Human red cell differentiation requires the action of erythropoietin on committed progenitor cells. In iron deficiency, committed erythroid progenitors lose responsiveness to erythropoietin, resulting in hypoplastic anemia. To address the basis for iron regulation of erythropoiesis, we established primary hematopoietic cultures with transferrin saturation levels that restricted erythropoiesis but permitted granulopoiesis and megakaryopoiesis. Experiments in this system identified as a critical regulatory element the aconitases, multifunctional iron-sulfur cluster proteins that metabolize citrate to isocitrate. Iron restriction suppressed mitochondrial and cytosolic aconitase activity in erythroid but not granulocytic or megakaryocytic progenitors. An active site aconitase inhibitor, fluorocitrate, blocked erythroid differentiation in a manner similar to iron deprivation. Exogenous isocitrate abrogated the erythroid iron restriction response in vitro and reversed anemia progression in iron-deprived mice. The mechanism for aconitase regulation of erythropoiesis most probably involves both production of metabolic intermediates and modulation of erythropoietin signaling. One relevant signaling pathway appeared to involve protein kinase Calpha/beta, or possibly protein kinase Cdelta, whose activities were regulated by iron, isocitrate, and erythropoietin.
Transition aware global signaling (TAGS) (2003)

We propose a new receiver to reduce the number of repeaters used in global wiring. The receiver stores the next state of the line while quiet. Upon detection of a transition at the end of the line the output is temporarily driven by the stored next state. Transitions at the output of the receiver are much faster than at the end of the line since they are generated locally. Using the TAGS receiver we can run a 15 mm line (180 nm node) at 800 MHz with no repeaters. The same line requires three repeaters with a traditional receiver and consumed more power and area. The TAGS receiver also outperforms a standard inverter at the 70 nm technology node. A noise analysis at the two technology nodes shows that the receiver maintains good functional noise immunity.
Fluorescence ratio imaging of dynamic intracellular ionic signals (1988)

Recent technical advances now enable the continuous imaging of important ionic signals inside individual living cells with micron spatial resolution and subsecond time resolution. This methodology relies on the molecular engineering of indicator dyes whose fluorescence is strong and highly sensitive to ions such as Ca 2+ , H + , or Na + , or Mg 2+ . The Ca 2+ indicators, exemplified by fura-2 and indo-1, derive their high affinity (K d near 200 nM) and selectivity for Ca 2+ to a versatile tetracarboxylate binding site 3 modeled on and isosteric with the well known chelator EGTA. The most commonly used pH indicators are fluorescein dyes (such as BCECF) modified to adjust their pK a 's and improve their retention inside cells. Na + indicators are crown ethers with cavity sizes chosen to select Na + over K + : Mg 2+ indicators use tricarboxylate binding sites truncated from those of the Ca 2+ chelators, resulting in a more compact arrangement of carboxylates to suit the smaller ion.
Bulk PZT Actuator for Parallel out-of-plane motion: The Superiority of Torsion Deformation over Bending Deformation (2016)

We present a new method for achieving a parallel out-of-plane (piston) motion, by using torsion actuation of a bulk, unimorph, piezoelectric actuator. Three piezoelectric spiral arms support a central stage, the arms are both poled and driven by interdigitated electrodes (IDEs) deposited directly on the top side of the structure. We show that when the IDEs are oriented at 90° relative to the spiral tangent, and thus only an internal moment is induced, the supported stage is only slightly displaced. In contrast, we show that when the IDEs are oriented at 45° relative to the spiral tangent, a torsional deformation is induced in the spiral arms, which results in a much larger (×20) displacement of the central stage. This demonstrates the superiority of torsion deformation in driving curved piezoelectric unimorph arms.
.kappa. Opioid Receptor Selective Affinity Labels: Electrophilic Benzeneacetamides as .kappa.-Selective Opioid Antagonists (1994)

2-(3,4-Dichlorophenyl)-N-methyl-N-[1-(3- or 4-substituted phenyl)-2-(1-pyrrolidinyl)ethyl]-acetamides 3-6 were synthesized as kappa-selective affinity labels and evaluated for opioid activity. In smooth muscle preparations, the non-electrophilic parent compound (+)-S-2 and the affinity labels 3-6 behaved as kappa agonists in that they were potently antagonized by norbinaltorphimine (norBNI). In addition to the high binding affinity and selectivity of the 3-isothiocyanate 3 (DIPPA) to kappa opioid receptors, wash studies have suggested that this involves covalent binding. In the mouse tail-flick assay, the 3- and 4-substituted isomers (3 and 5, respectively) produced long-lasting antagonism of the antinociceptive effect of the kappa opioid agonist, (+/-)-trans-2-(3,4-dichlorophenyl)-N-methyl-N-[2-(1-pyrrolidinyl) cyclohexyl]acetamide ((+/-)-U50,488). In contrast, the non-electrophilic parent compound (+)-S-2 and the fumaramate derivative 4 were devoid of antagonist activity in the tail-flick assay. At substantially different doses, DIPPA (3) and the 4-isothiocyanate 5 also produced antinociception in the mouse abdominal stretch assay. In addition, DIPPA and the 3-fumaramate methyl ester 4 had improved in vivo kappa-selectivities compared to the unsubstituted parent compound (+)-S-2 and the para-substituted derivative 5. The improved kappa-selectivities of 3 and 4 and the different agonist and antagonist potencies of 3 and 5 may be explained respectively by the existence of multiple kappa agonist binding sites and distinct agonist and antagonist binding sites. In view of the antagonist selectivity and the apparent irreversible binding of DIPPA to kappa receptors, it may serve as a useful pharmacologic or biochemical tool to investigate kappa opioid receptors.
Pump-to-Stokes transfer of relative intensity noise in Brillouin fiber ring lasers (2006)

We have experimentally investigated pump-to-Stokes intensity noise transfer in both the frequency domain and the time domain in all-fiber single-frequency Brillouin ring lasers. In the high-frequency region (>1MHz), the pump-to-Stokes noise transfer function can be much smaller than unity, indicating that the Brillouin fiber lasers act as an efficient low-pass filter. The maximum noise reduction of 40-60 dB was observed at antiresonant frequencies that are multiples of half the cavity free spectral range. This is the first experimental demonstration, to the authors' knowledge, of intensity noise reduction in Brillouin fiber lasers.
High-level production and purification of a fully active recombinant dextransucrase from<i>Leuconostoc mesenteroides</i>NRRL B-512F (2006)

Recombinant expression of the dextransucrase dsrS gene by Escherichia coli was optimized to produce 5850 U L(-1) (culture) of DSR-S, corresponding to a 30-fold increase compared with previous studies. Rational deletions of the signal peptide, the beginning of the variable region and the last four repeats of the C-terminal end caused no loss of activity. This new variant successfully purified was remarkably stable. With a k(cat) of 584 s(-1), it is the most efficient recombinant glucansucrase described to date. The synthesized polymer possesses more than 95% of alpha-1,6 links, like the dextran produced by the native enzyme, and innovative gel properties were obtained.
Stimulatory and Inhibitory Effects of Organohalides on the Dehalogenating Activities of PCB-Dechlorinating Bacterium <i>o</i>-17 (2006)

Bacterium o-17, a microorganism capable of the ortho dechlorination of 2,3,5,6-polychlorinated biphenyl (PCB), is a member of a sediment-free, nonmethanogenic mixed culture. The culture was examined for the ability to dechlorinate 26 PCB congeners, 12 chlorobenzenes (CBZs), and 6 chlorinated ethenes (CEs). Eight of the PCBs and 4 of the CBZs were dechlorinated including single-flanked ortho PCB chlorines, but double-flanked chlorines of PCBs and CBZs were preferentially dechlorinated. The dechlorination of three of the PCBs (2,3,4,5,6-, 2,3,4,6-, and 2,3,5,6-PCB), three of the CBZs (hexa-, penta-, and 1,2,3-CBZ), and PCE could be sustained for three or more sequential transfers of the bacterial community. Two PCBs (2,3,4- and 2,3,5-PCB), two CBZs (1,2,3,5- and 1,2,4,5-CBZ), and trichloroethene were dechlorinated only when a more extensively chlorinated parent compound was present. Aroclor 1260 and 2,4,6-PCB, not dechlorinated by the culture, inhibited the dechlorination of 2,3,5,6-PCB. Within the culture only bacterium o-17 was linked to dechlorination by PCR-DGGE analysis, confirming that this dehalogenating species was the catalyst for the dechlorination of the compounds tested. The microorganism is capable of dechlorinating several different congeners of PCBs, CBZs, and CEs, and it remains a rare example of an ortho-PCB dechlorinator. However, its limited ability to dechlorinate more extensively chlorinated congeners and Aroclor plus the inhibitory effects of some PCB congeners upon the bacterium is consistent with the observed infrequency of this reaction in the environment. An assessment of bioremediation potential of this microorganism in situ will require a greater understanding of the synergistic, cometabolic and competitive interactions of PCB dechlorinating microbial communities.
One-Dimensional Optoelectronic Nanostructures Derived from the Aqueous Self-Assembly of π-Conjugated Oligopeptides (2008)

The aqueous self-assembly of oligopeptide-flanked pi-conjugated molecules into discrete one-dimensional nanostructures is described. Unique to these molecules is the fact that the pi-conjugated unit has been directly embedded within the peptide backbone by way of a synthetic amino acid with pi-functionality that is compatible with standard Fmoc-based peptide synthesis. The peptide-based molecular design enforces intimate pi-pi communication within the aggregate after charge-screening and self-assembly, making these nanostructures attractive for optical or electronic applications in biological environments. The synthesis and assembly are reported along with spectroscopic and morphological characterization of the new nanomaterials.
In‐frame triplet deletions in<i>RHD</i>alter the D antigen phenotype (2006)

Partial D may be caused by a single-amino-acid deletion in RhD. The altered RhD protein segments in DVL types are adjacent to the extracellular loop 4, which constitutes one of the most immunogenic parts of the D antigen. These RhD protein segments are also altered in all DV, which may explain the similarity in phenotype. At the nucleotide level, the triplet deletions may have resulted from replication slippage. A total of nine amino acid positions in an Rhesus protein may be affected by this mechanism.