gebhart/patrepeval huggingface dataset:
741: A Very Small Proportion of Patients with Localized Prostate Cancer Shed Circulating Prostate Cells Using Real-Time RT-PCR for KLK2 and KLK3 Mrnas, which Enables Reliable CPC Detection in Metastatic Disease (2007)

You have accessJournal of UrologyPodium 25, Monday, May 21, 2007, 8:00 - 10:00 am1 Apr 2007741: A Very Small Proportion of Patients with Localized Prostate Cancer Shed Circulating Prostate Cells Using Real-Time RT-PCR for KLK2 and KLK3 Mrnas, which Enables Reliable CPC Detection in Metastatic Disease Pauliina Helo, Angel M. Serio, Maria Rissanen, Kim Pettersson, Daniel C. Danila, Martin Fleisher, Howard I. Scher, Peter T. Scardino, James A. Eastham, and Hans G. Lilja Pauliina HeloPauliina Helo More articles by this author , Angel M. SerioAngel M. Serio More articles by this author , Maria RissanenMaria Rissanen More articles by this author , Kim PetterssonKim Pettersson More articles by this author , Daniel C. DanilaDaniel C. Danila More articles by this author , Martin FleisherMartin Fleisher More articles by this author , Howard I. ScherHoward I. Scher More articles by this author , Peter T. ScardinoPeter T. Scardino More articles by this author , James A. EasthamJames A. Eastham More articles by this author , and Hans G. LiljaHans G. Lilja More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(18)30981-9AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail "741: A Very Small Proportion of Patients with Localized Prostate Cancer Shed Circulating Prostate Cells Using Real-Time RT-PCR for KLK2 and KLK3 Mrnas, which Enables Reliable CPC Detection in Metastatic Disease." The Journal of Urology, 177(4S), p. 249 © 2016 by American Urological AssociationFiguresReferencesRelatedDetails Volume 177Issue 4SApril 2007Page: 249 Advertisement Copyright & Permissions© 2016 by American Urological AssociationMetricsAuthor Information Pauliina Helo More articles by this author Angel M. Serio More articles by this author Maria Rissanen More articles by this author Kim Pettersson More articles by this author Daniel C. Danila More articles by this author Martin Fleisher More articles by this author Howard I. Scher More articles by this author Peter T. Scardino More articles by this author James A. Eastham More articles by this author Hans G. Lilja More articles by this author Expand All Advertisement PDF DownloadLoading ...
Double fiber probe with a single fiber Bragg grating based on the capillary-driven self-assembly fabrication method for dimensional measurement of micro parts (2015)

Focusing on the ultra-precision dimensional measurement of parts with micro-scale dimensions and high aspect ratios, a two-dimensional double fiber probe with a single fiber Bragg grating (DS-FBG probe) is investigated in detail in this paper. The theoretical analysis of the sensing principle is verified by spectrum simulations of the DS-FBG probe with a modified transfer matrix method using the strain distribution within the DS-FBG probe. The fabrication process and physical principle of the capillary-driven self-assembly of double fibers in the UV adhesive with a low viscosity are demonstrated. Experimental results indicate that resolutions of 30 nm in radial direction and 15 nm in axial direction can be achieved, and the short-term displacement drifts within 90 seconds are 28.0 nm in radial direction and 7.9 nm in axial direction, and the long-term displacement drifts within 1 hour are 61.3 nm in radial direction and 17.3 nm in axial direction. The repeatability of the probing system can reach 60 nm and the measurement result of a standard nozzle is 300.49 μm with a standard deviation of 20 nm.
Pathogenic <i>Leptospira</i> species express surface‐exposed proteins belonging to the bacterial immunoglobulin superfamily (2003)

Proteins with bacterial immunoglobulin-like (Big) domains, such as the Yersinia pseudotuberculosis invasin and Escherichia coli intimin, are surface-expressed proteins that mediate host mammalian cell invasion or attachment. Here, we report the identification and characterization of a new family of Big domain proteins, referred to as Lig (leptospiral Ig-like) proteins, in pathogenic Leptospira. Screening of L. interrogans and L. kirschneri expression libraries with sera from leptospirosis patients identified 13 lambda phage clones that encode tandem repeats of the 90 amino acid Big domain. Two lig genes, designated ligA and ligB, and one pseudogene, ligC, were identified. The ligA and ligB genes encode amino-terminal lipoprotein signal peptides followed by 10 or 11 Big domain repeats and, in the case of ligB, a unique carboxy-terminal non-repeat domain. The organization of ligC is similar to that of ligB but contains mutations that disrupt the reading frame. The lig sequences are present in pathogenic but not saprophytic Leptospira species. LigA and LigB are expressed by a variety of virulent leptospiral strains. Loss of Lig protein and RNA transcript expression is correlated with the observed loss of virulence during culture attenuation of pathogenic strains. High-pressure freeze substitution followed by immunocytochemical electron microscopy confirmed that the Lig proteins were localized to the bacterial surface. Immunoblot studies with patient sera found that the Lig proteins are a major antigen recognized during the acute host infection. These observations demonstrate that the Lig proteins are a newly identified surface protein of pathogenic Leptospira, which by analogy to other bacterial immunoglobulin superfamily virulence factors, may play a role in host cell attachment and invasion during leptospiral pathogenesis.
High‐activity enzyme‐polyurethane coatings (2002)

The synthesis of water-borne polyurethane coatings in the presence of diisopropylfluorophosphatase (DFPase, E.C. 3.8.2.1) enabled the irreversible attachment of the enzyme to the polymeric matrix. The distribution of immobilized DFPase as well as activity retention are homogeneous within the coating. The resulting enzyme-containing coating (ECC) film hydrolyzes diisopropylfluorophosphate (DFP) in buffered media at high rates, retaining approximately 39% intrinsic activity. Decreasing ECC hydrophilicity, via the use of a less hydrophilic polyisocyanate during polymerization, significantly enhanced the intrinsic activity of the ECC. DFPase-ECC has biphasic deactivation kinetics, where the initial rapid deactivation of DFPase-ECC leads to the formation of a hyperstable and active form of enzyme.
Flash hydrogenation of coal. [3 claims; 2 drawings; fluidized bed] (1976)

A process is described for the hydrogenation of coal comprising the contacting of powdered coal with hydrogen in a rotating fluidized bed reactor. A rotating fluidized bed reactor suitable for use in this process is also disclosed. The coal residence time in the reactor is limited to less than 5 seconds while the hydrogen contact time is not in excess of 0.2 seconds. 3 claims, 2 drawing figures.
Stiffness-matched segmented metallic guidewire for interventional cardiovascular MRI (2015)

Background Conductive guidewires and intravascular catheters are at risk of RF-induced heating under MRI [1]. Heating is found predominantly at the tip of conductive wires [2], and is modulated by wire diameter, length and insulation thickness [3]. Non-conductive materials, such as polymer, impart unsatisfactory mechanical properties on guidewires in terms of flexibility, stiffness, and torquability, for navigating tortuous cardiovascular structures and for safely delivering catheter devices.
Novel T cell therapy using spherical nucleic acids (2018)

Abstract Immune stimulating Spherical Nucleic Acids (SNAs) are nanoparticles comprised of a spherical, hollow, lipid core surrounded by an oligonucleotide shell, allowing for the simultaneous delivery of tumor antigens and immune stimulating oligonucleotides into immune cells. Interestingly, we have found that SNAs can readily enter primary nonphagocytes such as T lymphocytes. The uptake of SNAs increases expression of co-stimulatory molecules and presentation of antigen via MHC class I molecules by T cells. SNA treated T cells are endowed with the ability to prime tumor antigen specific CD8+T cells in a long lasting and durable manner compared to "free" oligonucleotide and antigen admix-treated T cells. Furthermore, SNA treated T cells are able to act as cellular chaperones and transfer both SNA-derived oligonucleotide and antigen to other cells. In an amplifying cascade manner, these "bystander" cells, which receive SNA material, then gain the ability to prime antigen-specific CD8+ T cells and achieve stronger antitumor effect. The cellular chaperone cascade only occurs when cells are treated with SNAs compared to the "free" material. The unique structural properties of SNAs thus confer a set of key biological properties that make SNAs particularly compelling for the efficient and specific delivery of tumor antigen and adjuvant in adoptive T cell therapy.
A Multi-Path Gated Ring Oscillator TDC With First-Order Noise Shaping (2009)

An 11-bit, 50-MS/s time-to-digital converter (TDC) using a multipath gated ring oscillator with 6 ps of effective delay per stage demonstrates 1st-order noise shaping. At frequencies below 1 MHz, the TDC error integrates to 80 fs (rms) for a dynamic range of 95 dB with no calibration required. The 157 times 258 mum TDC is realized in 0.13 mum CMOS and, depending on the time difference between input edges, consumes 2.2 to 21 mA from a 1.5 V supply.
Antireflective surface structures on optics for high energy lasers (2014)

We report results for antireflective surface structures (ARSS) fabricated directly into the surface of optics and lenses which are important as high energy (multi-kW) laser components, including fused silica windows and lenses, YAG crystals and ceramics and spinel ceramics. Very low reflection losses as well as high laser damage thresholds have been measured for optics with ARSS. Progress to scale up the process for large size windows will also be presented..
Ghrelin, a Natural GH Secretagogue Produced by the Stomach, Induces Hyperglycemia and Reduces Insulin Secretion in Humans (2001)

Ghrelin, a 28 amino acid gastric hormone is a natural ligand of the GH Secretagogue (GHS) receptor (GHS-R) and strongly stimulates GH secretion though, like synthetic GHS, it shows other endocrine and non-endocrine activities. Aim of the present study was to clarify whether ghrelin administration influences insulin and glucose levels in humans. To this goal, we compared the effects of ghrelin, hexarelin, a synthetic GHS, or placebo on insulin and glucose as well as on GH levels in 11 normal young volunteers (age [mean +/- SEM]: 28.5 +/- 3.1 yr; BMI: 22.2 +/- 0.9 Kg/m(2)). Ghrelin induced very marked increase in GH secretion (DeltaAUC(0-180): 5777.1 +/- 812.6 microg/l/h; p < 0.01) which was not modified by placebo. Placebo administration did not modify insulin and glucose levels. On the other hand, ghrelin administration induced a prompt increase in glucose levels (DeltaAUC(0-180): 1343.1 +/- 443.5 mg/dl/h; p < 0.01 vs. saline). Absolute glucose levels at +15' were already higher than those at baseline (93.9 +/- 7.1 mg/dl; p < 0.01) and persisted elevated up to 165' (90.3 +/- 5.8 mg/dl; p < 0.01 vs. 0'). Ghrelin administration was also followed by a decrease in serum insulin levels (DeltaAUC(0-180): -207.1 +/- 70.5 mU/l/h; p < 0.05 vs. saline). Absolute insulin levels were significantly reduced from 30' (11.4 +/- 0.9 mU/l, p < 0.1 vs. 0'), showed the nadir at +45' (10.0 +/- 0.6 mU/l, p < 0.01 vs. 0') and then persisted lower (p < 0.01) than baseline up to +105'. Hexarelin administration did not modify glucose and insulin levels despite its marked GH-releasing effect (DeltaAUC(0-180): 4156.8 +/- 1180.3 microg/l/h; p < 0.01 vs. saline) that was slightly lower (p < 0.05) than that of ghrelin. In conclusion, these findings show that, besides stimulating GH secretion, ghrelin is a gastric hormone possessing metabolic actions such as hyperglycemic effect and lowering effect on insulin secretion in humans, at least after acute administration.
Retinal Imaging by Spectral Optical Coherence Tomography (2007)

SOCT detects small disturbances of the retinal structure and helps to precisely determine layers involved in different pathologies.
Peg-Free Hand Shape Verification Using High Order Zernike Moments (2006)

Hand-based verification is a key biometric technology with a wide range of potential applications both in industry and government. The focus of this work is on improving the efficiency, accuracy, and robustness of hand-based verification. In particular, we propose using high-order Zernike moments to represent hand geometry, avoiding the more difficult and prone to errors process of hand-landmark extraction (e.g., finding finger joints). The proposed system operates on 2D hand silhouette images acquired by placing the hand on a planar lighting table without any guidance pegs, increasing the ease of use compared to conventional systems. Zernike moments are powerful translation, rotation, and scale invariant shape descriptors. To deal with several practical issues related to the computation of highorder Zernike moments including computational cost and lack of accuracy due to numerical errors, we have employed an efficient algorithm that uses arbitrary precision arithmetic, a look-up table, and avoids recomputing the same terms multiple times [2]. The proposed hand-based authentication system has been tested on a database of 40 subjects illustrating promising results. Qualitative comparisons with state of the art systems illustrate comparable of better performance.
Adipose-Derived Cell Construct Stabilizes Heart Function and Increases Microvascular Perfusion in an Established Infarct (2013)

Abstract We have previously shown that myocardial infarction (MI) immediately treated with an epicardial construct containing stromal vascular fraction (SVF) from adipose tissue preserved microvascular function and left ventricle contractile mechanisms. In order to evaluate a more clinically relevant condition, we investigated the cardiac recovery potential of an SVF construct implanted onto an established infarct. SVF cells were isolated from rat adipose tissue, plated on Vicryl, and cultured for 14 days. Fischer-344 rats were separated into MI groups: (a) 6-week MI (MI), (b) 6-week MI treated with an SVF construct at 2 weeks (MI SVF), (c) 6-week MI with Vicryl construct at 2 weeks (MI Vicryl), and (d) MI 2wk (time point of intervention). Emax, an indicator of systolic performance and contractile function, was lower in the MI and MI Vicryl versus MI SVF. Positron emission tomography imaging (18F-fluorodeoxyglucose) revealed a decreased percentage of relative infarct volume in the MI SVF versus MI and MI Vicryl. Total vessel count and percentage of perfusion assessed via immunohistochemistry were both increased in the infarct region of MI SVF versus MI and MI Vicryl. Overall cardiac function, percentage of relative infarct, and percentage of perfusion were similar between MI SVF and MI 2wk; however, total vessel count increased after SVF treatment. These data suggest that SVF treatment of an established infarct stabilizes the heart at the time point of intervention by preventing a worsening of cardiac performance and infarcted volume, and is associated with increased microvessel perfusion in the area of established infarct.
Zika Virus: Diagnostics for an Emerging Pandemic Threat (2016)

Zika virus (ZIKV) is anAedesmosquito-borne flavivirus that emerged in Brazil in 2015 and then rapidly spread throughout the tropical and subtropical Americas. Based on clinical criteria alone, ZIKV cannot be reliably distinguished from infections with other pathogens that cause an undifferentiated systemic febrile illness, including infections with two common arboviruses, dengue virus and chikungunya virus. This minireview details the methods that are available to diagnose ZIKV infection.
Sequence analysis of hepatitis C virus genotypes 1 to 5 reveals multiple novel subtypes in the Benelux countries (1995)

Hepatitis C virus (HCV) isolates from a cohort of 315 patients from the Benelux countries (Belgium, The Netherlands, Luxembourg) were genotyped by means of reverse hybridization Inno-LiPA (line probe assay). Genotypes 1a, 1b, 2a, 2b, 3a, 4a and 5a were detected. From the cohort, isolates representing all types and those showing an aberrant LiPA pattern were further analysed by sequencing parts of the 5' UTR, core (nt 1 to 326; aa residues 1 to 108) and core/E1 (nt 477 to 924; aa residues 159 to 308) regions. Molecular evolutionary analysis of the core and core/E1 regions allowed discrimination between known and additional subtypes, especially within types 2 and 4. The core region is not suitable for classification of new subtypes because of the relatively high level of conservation. The core/E1 region displays a higher level of sequence variation and allows much more distinct discrimination between subtypes. Genotypes 2 and 4 are particularly heterogeneous, with at least 7 and 10 subtypes, respectively. In contrast to previous reports from Europe, HCV isolates from the cohort constituted a highly heterogeneous population of virus variants, especially within genotypes 2 and 4.
Balanced-Rotating-Spray Tank-And-Pipe-Cleaning System (1995)

Spray head translates and rotates to clean entire inner surface of tank or pipe. Cleansing effected by three laterally balanced gas/liquid jets from spray head that rotates about longitudinal axis. Uses much less liquid. Cleaning process in system relies on mechanical action of jets instead of contaminant dissolution. Eliminates very difficult machining needed to make multiple converging/diverging nozzles within one spray head. Makes nozzle much smaller. Basic two-phase-flow, supersonic-nozzle design applied to other spray systems for interior or exterior cleaning.
FPGA PLB Architecture Evaluation and Area Optimization Techniques Using Boolean Satisfiability (2007)

This paper presents a field-programmable gate array (FPGA) logic synthesis technique based upon Boolean satisfiability. This paper shows how to map any Boolean function into an arbitrary programmable logic block (PLB) architecture without any custom decomposition techniques. The authors illustrate several useful applications of this technique by showing how this technique can be used for architecture evaluation and area optimization. When evaluating the FPGA architecture, the authors focus on the basic building block of the FPGA, which they refer to as PLB. In order to illustrate the flexibility of their evaluation framework, several unrelated PLB architectures are evaluated in an automated fashion. Furthermore, the authors show that using their technique is able to reduce FPGA resource usage by 27% on average in common subcircuits found in digital design.
Proinflammatory Proteases Liberate a Discrete High-Affinity Functional FPRL1 (CCR12) Ligand from CCL23 (2007)

Most chemokines have been found to bind to and signal through single or highly related chemokine receptors. However, a single chemokine protein, a processed form of the alternatively spliced CCL23 (CKbeta8/MPIF-1) gene product, potently engages both the "classical" chemokine receptor CCR1, as well as FPRL1, a type of pattern recognition receptor on innate immune cells. However, the mechanism by which the alternative form of CCL23 is processed is unknown. In this study, we show that proteases associated with inflammation cleave CCL23 immediately N-terminal to the 18-residue domain encoded by the alternatively spliced nucleotides, resulting in potent CCR1 and FPRL1 activity. The proteases also cleave CCL23 immediately C-terminal to the inserted domain, producing a typical CC chemokine "body" containing even further-increased CCR1 potency and a released approximately 18-aa peptide with full FPRL1 activity but no activity for CCR1. This peptide, which we term SHAAGtide, is by itself an attractant of monocytes and neutrophils in vitro, recruits leukocytes in vivo, and is 50- to 100-fold more potent than all other natural agents posited to act on FPRL1. The appearance of SHAAGtide appears to be transient, however, as the proinflammatory proteases subsequently cleave within the peptide, abolishing its activity for FPRL1. The sequential activation of a transient FPRL1 ligand and a longer-lived CCR1 ligand within a single chemokine may have important consequences for the development of inflammation or the link between innate and adaptive immunity.
Development of panel structure for a high-resolution 21-in-diagonal full-color surface-discharge plasma display panel (2000)

Plasma display panel (PDP) structure has been investigated to develop a 21-in-diagonal color plasma display with a high-resolution of 0.22-mm subpixel pitch. To realize a high-resolution display, a stripe alignment of three primary color elements such as red, blue, and green, was introduced. Four kinds of panel structures are compared and finally stripe rib and stripe phosphor structures were introduced for the three electrode surface discharge plasma display. The 21-in-plasma display developed with the structure has firstly been put into the market with sufficient performance, such as a wide operating voltage margin, a high luminance, and a wide viewing angle of more than 60 V, 200 cd/m/sup 2/, and 160/spl deg/, respectively.
Data from Spatial Architecture and Arrangement of Tumor-Infiltrating Lymphocytes for Predicting Likelihood of Recurrence in Early-Stage Non–Small Cell Lung Cancer (2023)

<div>AbstractPurpose:<p>The presence of a high degree of tumor-infiltrating lymphocytes (TIL) has been proven to be associated with outcome in patients with non–small cell lung cancer (NSCLC). However, recent evidence indicates that tissue architecture is also prognostic of disease-specific survival and recurrence. We show a set of descriptors (spatial TIL, SpaTIL) that capture density, and spatial colocalization of TILs and tumor cells across digital images that can predict likelihood of recurrence in early-stage NSCLC.</p>Experimental Design:<p>The association between recurrence in early-stage NSCLC and SpaTIL features was explored on 301 patients across four different cohorts. Cohort D<sub>1</sub> (<i>n</i> = 70) was used to identify the most prognostic SpaTIL features and to train a classifier to predict the likelihood of recurrence. The classifier performance was evaluated in cohorts D<sub>2</sub> (<i>n</i> = 119), D<sub>3</sub> (<i>n</i> = 112), and D<sub>4</sub> (<i>n</i> = 112). Two pathologists graded each sample of D<sub>1</sub> and D<sub>2</sub>; intraobserver agreement and association between manual grading and likelihood of recurrence were analyzed.</p>Results:<p>SpaTIL was associated with likelihood of recurrence in all test sets (log-rank <i>P</i> < 0.02). A multivariate Cox proportional hazards analysis revealed an HR of 3.08 (95% confidence interval, 2.1–4.5, <i>P</i> = 7.3 × 10<sup>−5</sup>). In contrast, agreement among expert pathologists using tumor grade was moderate (Kappa = 0.5), and the manual TIL grading was only prognostic for one reader in D<sub>2</sub> (<i>P</i> = 8.0 × 10<sup>−3</sup>).</p>Conclusions:<p>A set of features related to density and spatial architecture of TILs was found to be associated with a likelihood of recurrence of early-stage NSCLC. This information could potentially be used for helping in treatment planning and management of early-stage NSCLC.</p><p><i>See related commentary by Peled et al., p. 1449</i></p></div>
Haplotype analysis in autosomal dominant polycystic kidney disease. (1995)

Haplotype analysis was performed in 35 autosomal dominant polycystic kidney disease (ADPKD) families typed with 13 markers close to the PKD1 locus. The identification of recombinants close to the PKD1 gene on chromosome 16p indicates that PKD1 lies between CMM65 distally and 26-6 proximally. In addition, three unlinked (PKD2) families and two families with potential new mutation were identified.