gebhart/patrepeval huggingface dataset:
New aspects of influenza viruses (1992)

Influenza virus infections continue to cause substantial morbidity and mortality with a worldwide social and economic impact. The past five years have seen dramatic advances in our understanding of viral replication, evolution, and antigenic variation. Genetic analyses have clarified relationships between human and animal influenza virus strains, demonstrating the potential for the appearance of new pandemic reassortants as hemagglutinin and neuraminidase genes are exchanged in an intermediate host. Clinical trials of candidate live attenuated influenza virus vaccines have shown the cold-adapted reassortants to be a promising alternative to the currently available inactivated virus preparations. Modern molecular techniques have allowed serious consideration of new approaches to the development of antiviral agents and vaccines as the functions of the viral genes and proteins are further elucidated. The development of techniques whereby the genes of influenza viruses can be specifically altered to investigate those functions will undoubtedly accelerate the pace at which our knowledge expands.
Protein Digestion, Ultrafiltration, and Size Exclusion Chromatography to Optimize the Isolation of Exosomes from Human Blood Plasma and Serum (2018)

Exosomes, a type of nanovesicle released from all cell types, can be isolated from any bodily fluid. The contents of exosomes, including proteins and RNAs, are unique to the cells from which they are derived and can be used as indicators of disease. Several common enrichment protocols, including ultracentrifugation, yield exosomes laden with soluble protein contaminants. Specifically, we have found that the most abundant proteins within blood often co-purify with exosomes and can confound downstream proteomic studies, thwarting the identification of low abundance biomarker candidates. Of additional concern is irreproducibility of exosome protein quantification due to inconsistent representation of non-exosomal protein levels. The protocol detailed here was developed to remove non-exosomal proteins that co-purify along with exosomes, adding rigor to the exosome purification process. Five methods were compared using paired blood plasma and serum from five donors. Analysis using nanoparticle tracking analysis and micro bicinchoninic acid protein assay revealed that a combined protocol utilizing ultrafiltration and size exclusion chromatography yielded the optimal vesicle enrichment and soluble protein removal. Western blotting was used to verify that the expected abundant blood proteins, including albumin and apolipoproteins, were depleted.
Poly(fumaric-co-sebacic) microspheres as oral drug delivery systems (1996)

The current study focuses on the development of bioadhesive oral delivery systems based on bioerodible polyanhydrides. The polymers were studied and characterized using a novel tensiometer based on a very sensitive electrobalance. The system was designed to mimic in vivo interactions, thus all experiments were conducted with freshly excised tissue immersed in physiological saline at 37 degrees C. Poly(fumaric-co-sebacic) [P(FA:SA)] was found to be the most bioadhesive polymer from a series of different thermoplastic materials evaluated. Correlation with in vivo performance was investigated by determining gastrointestinal (GI) residence time of barium-loaded microspheres. Residence times of 24 to 36 h provided a strong indication that these microspheres were good candidates for bioadhesive drug delivery systems. To evaluate the effect of these materials on bioavailability, the anticoagulant drug, dicumarol, was encapsulated. Systemic blood levels demonstrated increased bioavailability for the encapsulated dicumarol formulation as compared with unencapsulated drug. (c) 1996 John Wiley & Sons, Inc.
Novel α-Amino-3-hydroxy-5-methylisoxazole-4-propionate Receptor Antagonists:  Synthesis and Structure−Activity Relationships of 6-(1<i>H</i>-Imidazol-1-yl)- 7-nitro-2,3(1<i>H</i>,4<i>H</i>)-pyrido[2,3-<i>b</i>]pyrazinedione and Related Compounds (1996)

We have synthesized and evaluated azaquinoxalinediones 3a-c for their activity in inhibiting [3H]AMPA binding from rat whole brain. It was found that the azaquinoxalinedione nucleus functions as a bioisostere for quinoxalinedione in AMPA receptor binding. The detailed structure-activity relationships of 6- and/or 7-substituted 2,3(1H,4H)-pyrido[2,3-b]pyrazinedione derivatives 4, 7-1-, 13, 15 and 16 showed some differences in comparison with those of the corresponding substituted quinoxalinediones, including 6-(1H-imidazol-1-yl)-7-nitro-2,3-(1H,4H)-quinoxalinedione (1) (YM90K). The X-ray study exhibited that conformation of the 7-nitro group of 1.HCl was nearly coplanar with the quinoxaline ring, whereas the 6-imidazol-1-yl group was rotated with respect to the aromatic ring. From the glycine site on NMDA receptor binding study, it is indicated that bulkiness of 6-substituents on pyridopyrazinediones may be responsible for the selectivity against the glycine site. Among the series of azaquinoxalinediones, 6-(1H-imidazol-1-yl)-7-nitro-2,3(1H,4H)-pyrido[2,3-b]pyrazinedione (8c) exhibited a combination of the best affinity to AMPA receptors with a Ki value of 0.14 microM and selectivity against the glycine site (no affinity at 10 microM). In vivo, 8c also protected against sound-induced seizure in DBA/2 mice (minimum effective dose, 10 mg/kg ip).
A new scheme for MC-CDMA systems in the presence of imperfections (2002)

The combination of code division multiple access (CDMA) and multi-carrier (MC) modulation has been proposed for high rate wireless communications. The main idea is to transmit the high data rate stream on a number of parallel lower rate channels. The long symbol duration associated with each subcarrier makes the system more susceptible to various imperfections, such as frequency offset, phase noise and fast fading, that destroy the orthogonality between the subcarriers and degrade performance. In this paper, we propose a novel and simple receiver structure to address the effects of imperfections. The proposed receiver fully compensates for the frequency offset and phase noise effects and, unlike existing designs, exploits temporal channel variations to attain a higher level of diversity, thereby improving system performance over that under slow fading.
Multiplexed Microcolumn-Based Process for Efficient Selection of RNA Aptamers (2013)

We describe a reusable microcolumn and process for the efficient discovery of nucleic acid aptamers for multiple target molecules. The design of our device requires only microliter volumes of affinity chromatography resin-a condition that maximizes the enrichment of target-binding sequences over non-target-binding (i.e., background) sequences. Furthermore, the modular design of the device accommodates a multiplex aptamer selection protocol. We optimized the selection process performance using microcolumns filled with green fluorescent protein (GFP)-immobilized resin and monitoring, over a wide range of experimental conditions, the enrichment of a known GFP-binding RNA aptamer (GFPapt) against a random RNA aptamer library. We validated the multiplex approach by monitoring the enrichment of GFPapt in de novo selection experiments with GFP and other protein preparations. After only three rounds of selection, the cumulative GFPapt enrichment on the GFP-loaded resin was greater than 10(8) with no enrichment for the other nonspecific targets. We used this optimized protocol to perform a multiplex selection to two human heat shock factor (hHSF) proteins, hHSF1 and hHSF2. High-throughput sequencing was used to identify aptamers for each protein that were preferentially enriched in just three selection rounds, which were confirmed and isolated after five rounds. Gel-shift and fluorescence polarization assays showed that each aptamer binds with high-affinity (KD < 20 nM) to the respective targets. The combination of our microcolumns with a multiplex approach and high-throughput sequencing enables the selection of aptamers to multiple targets in a high-throughput and efficient manner.
Built-in selectors self-assembled into memristors (2016)

We demonstrate an approach to build a selector into ReRAM (memristors) using engineered materials. In this approach, a segment(s) of “nonlinear material” is self-assembled into the conduction channel (s) (filament) of a memristor. The nonlinear material exhibits a highly nonlinear current-voltage characteristic, which gives rise to a nonlinear i-v characteristic of the memristor in the ON state.
Vaccination of cattle with a DNA plasmid encoding the bovine viral diarrhoea virus major glycoprotein E2 (1999)

Bovine viral diarrhoea virus (BVDV) is an economically important pathogen of cattle that is ubiquitously distributed worldwide. In this study, cattle were immunized by intramuscular injections with plasmid DNA expressing the BVDV type 1 major glycoprotein E2. Animals either received injections of naked DNA (N-DNA) or DNA in cationic liposomes (L-DNA). Both DNA preparations induced virus-specific neutralizing antibodies in vaccinates, although the response was much lower in N-DNA-immunized animals. N-DNA-vaccinated animals also showed virus-specific lymphocyte proliferation responses to type 1, live BVDV in vitro, whereas L-DNA vaccination induced no such responses. After 16 weeks, DNA-vaccinated and mock-vaccinated animals were challenged with a USDA-certified BVDV type 1 strain. Four significant observations were made: (1) N-DNA-vaccinated calves showed limited protection from virus challenge, (2) L-DNA-vaccinated animals did not show any signs of protection, (3) the challenge induced strong memory responses in the production of serum neutralizing antibodies to both genotypes (type 1 and 2 of BVDV), and (4) the challenge induced a mucosal memory response in nasal secretions of both L- and N-DNA-vaccinated animals.
ChemInform Abstract: A General Method for Copper‐Catalyzed Arylation of Arene C—H Bonds. (2009)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Irregular low-density parity-check code design based on integer lattices (2003)

We propose an irregular low-density parity-check (LDPC) code design scheme by extension of a regular LDPC code using Latin square matrices of random sequence. Our scheme is a deterministic method that enables to easily design good irregular LDPC code for any code rate and degree distributions. We optimize LDPC codes by Gaussian approximation (GA) Chung, S-Y et al., (2001) method and construct irregular LDPC codes based on integer lattices Vasic, B et al., (2002).
Numerical Optimization of Electron Beams for High Brightness x- and γ-Ray Production (2006)

Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter Facebook Reddit LinkedIn Tools Icon Tools Reprints and Permissions Cite Icon Cite Search Site Citation David J. Gibson, Scott G. Anderson, Frederic V. Hartemann, Craig W. Siders, Aaron M. Tremaine, Christopher P. J. Barty; Numerical Optimization of Electron Beams for High Brightness x‐ and γ‐Ray Production. AIP Conf. Proc. 27 November 2006; 877 (1): 602–608. https://doi.org/10.1063/1.2409190 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAIP Publishing PortfolioAIP Conference Proceedings Search Advanced Search |Citation Search
High-helical-pitch, cone-beam computed tomography (1998)

The object of a helical, cone-beam computed tomography, HCBCT, system is faster scan times for the volume of interest for a patient. The maximum helical-pitch that still produces good quality images limits the reduction in scan time. Although this is a three-dimensional problem, an extension of the completeness condition for a two-dimensional fan-beam reconstruction method serves as a guide to estimate the maximum helical-pitch. In HCBCT, each pixel in an arbitrary image slice is irradiated over a different range of gantry rotation angles. This leads to the idea of a different, or inconsistent, range of backprojection angles for each pixel in order to increase the helical-pitch while satisfying the completeness condition. Using inconsistent backprojection, the normalized helical-pitch ratio increases from 0.90 to 1.40 (for a source radius of 600 mm and field of view of 500 mm), where the normalized helical-pitch ratio is the linear advance of the patient couch per gantry revolution to the full axial height of the area detector (as projected at the isocentre).
Free Radical‐Mediated Aryl Amination and Its Use in a Convergent [3 + 2] Strategy for Enantioselective Indoline α‐Amino Acid Synthesis. (2003)

Abstract For Abstract see ChemInform Abstract in Full Text.
Rapid Detection of <i>Phytophthora infestans</i> in Late Blight-Infected Potato and Tomato Using PCR (1997)

Late blight caused by the oomycete pathogen Phytophthora infestans is a devastating disease of potato and tomato worldwide. A rapid and accurate method for specific detection of P. infestans is necessary for determination of late blight in infected fruit, leaves, and tubers. Ribosomal DNA (rDNA) from four isolates of P. infestans representing the four genotypes US1, US6, US7, and US8 was amplified using polymerase chain reaction (PCR) and the universal primers internal transcribed spacer (ITS) 4 and ITS5. PCR products were sequenced using an automated sequencer. Sequences were aligned with published sequences from 5 other Phytophthora species, and a region specific to P. infestans was used to construct a PCR primer (PINF). Over 140 isolates representing 14 species of Phytophthora and at least 13 other genera of fungi and bacteria were used to screen the PINF primer. PCR amplification with primers PINF and ITS5 results in amplification of an approximately 600 base pair product with only isolates of P. infestans from potato and tomato, as well as isolates of P. mirabilis and P. cactorum. P. mirabilis and P. cactorum are not pathogens of potato; however, P. cactorum is a pathogen of tomato. P. infestans and P. cactorum were differentiated by restriction digests of the amplified product. The PINF primer was used with a rapid NaOH lysis technique for direct PCR of P. infestans from infected tomato and potato field samples. The PINF primer will provide a valuable tool for detection of P. infestans in potatoes and tomatoes.
Event clustering & event series characterization on expected frequency (2017)

We present an efficient clustering algorithm applicable to one-dimensional data such as e.g. a series of times-tamps. Given an expected frequency ΔT <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-1</sup> , we introduce an O(N)-efficient method of characterizing N events represented by an ordered series of timestamps t <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">1</sub> , t <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> ,..., t <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">N</sub> . In practice, the method proves useful to e.g. identify time intervals of missing data or to locate isolated events. Moreover, we define measures to quantify a series of events by varying ΔT to e.g. determine the quality of an Internet of Things service.
Whispering-Gallery-Mode Tunable Narrow-Band-Pass Filter (2004)

Two hundred arteriographies of the feet performed after injuries were studied. The patients had no arterial disease. Various patterns of the vascularisation of the feet are described. The authors emphasize the frequent variations of distribution which are sometimes different from the usual pattern but are however not pathological and can be explained by hemodynamic disturbances. The authors also describe some anatomical criteria which are useful to identify the different arteries of the feet on the arteriographies with different positions of the feet. These criteria will be very useful with digital angiography.
Instrumentation for Measuring Continuous Oxygen Consumption of Surgical Patients (1977)

A system has been developed for continuously monitoring the oxygen consumption (V02) of surgical patients. A replenishment technique is used whereby the oxygen removed by the patient from a closed rebreathing circuit is replaced. This is accomplished using a feedback-controlled pump to add oxygen at a rate necessary to maintain a constant inspired oxygen percent. A second feedback loop adds nitrous oxide to the circuit at a rate equal to the patient's nitrous oxide uptake rate, thus maintaining a constant circuit volume. These two feedback loops constitute a system to monitor oxygen and nitrous oxide uptake during nitrous oxide anesthesia. The instrument responds to a step change in V02 in 4 min. System accuracy has been shown to be ±6% in an in vivo comparison, the major error resulting from oxygen sensor or electronics dnift, leaks in the system, or changes in residual volume. The record of a surgical patient's VO2 while under balanced anesthesia shows a practical application of the system.
Ventricular activation patterns during intrinsic conduction and right ventricular pacing in cardiac resynchronization therapy patients (2021)

Abstract Background Cardiac resynchronization therapy (CRT) involves stimulation of both right ventricle (RV) and left ventricle (LV). LV pacing from the sites of delayed electrical activation improves CRT response. The RV‐LV conduction is typically measured in intrinsic rhythm. The differences in RV‐LV conduction patterns and timing between intrinsic rhythm and during paced RV activation, these differences are not fully understood. Methods Enrolled patients were implanted with a de novo CRT device and quadripolar LV lead, with lead implant locations at the implanting physician's discretion. QRS duration and conduction delay between the RV lead and each of the four LV electrodes (D1, M2, M3, and P4) were measured during intrinsic conduction and RV pacing. Results Conduction measurements were collected from 275 patients across 14 international centers (68 ± 13 years of age, 73% male, 45% ischemic, 158 ± 22 ms QRS duration). Mean RV‐LV conduction time was shorter during intrinsic conduction versus RV pacing by 59.6 ms (106.5 ± 36.5 versus 166.1 ± 32.1 ms, p < 0.001). The intra‐LV activation delay between the latest and earliest activating LV electrode was also shorter during intrinsic conduction versus RV pacing by 6.6 ms (20.6 ± 13.1 vs. 27.2 ± 21.2 ms, p < 0.001). Intrinsic conduction and RV pacing resulted in a different activation order in 72.7% of patients, and the same LV activation order in 27.3%. Conclusions Differences in RV‐LV conduction time, intra‐LV conduction time, and activation pattern were observed between intrinsic conduction and RV pacing. These findings highlight the importance of evaluating intrinsic versus paced ventricular activation to guide LV pacing site selection in CRT patients.
Controlled-inversion transistors (1975)

Current-voltage characteristics similar to those of a semiconductor-controlled rectifier have been observed in three-terminal devices which have only a single p-n junction. The structure investigated is a layered sequence of metal/conducting ’’insulator’’/p-silicon/n+-silicon with electrical terminals at the metal and both sides of the junction. If a positive bias is applied to the metal the device can reside in either a low- or a high-impedance state. These states are brought about by controlling the presence or absence of the inversion layer at the insulator-silicon interface. Experimental data demonstrate the efficiency of this control permitted by the third intermediate terminal.
A hypoallergenic peptide mix containing T cell epitopes of the clinically relevant house dust mite allergens (2019)

IgG antibodies in HDM-allergic patients recognize peptide epitopes which are different from the epitopes recognized by IgE. This may explain why naturally occurring allergen-specific IgG antibodies do not protect against IgE-mediated allergic inflammation. A mix of hypoallergenic peptides containing T cell epitopes of the most important HDM allergens was identified.
Frequency metrology by use of quantum interference (1996)

Quantum interference in the rate of two-photon excitation of the 6S((1/2)) ?6P(3/2) ? 6D(5/2) transition in atomic cesium is exploited to demonstrate phase-sensitive frequency demodulation for an optical interval of +/- 12.5 THz. By thus using atoms as ultrafast nonlinear mixing elements, we suggest and analyze a new avenue for absolute comparisons of a dense set of frequencies over the range of 200-2000 nm.
Dynamic Control of the Flow of Terahertz Light (2009)

Guided propagation of THz light has been intensely developed in the recent years. He we describe our recent efforts towards dynamic, optical control of the flow of light in waveguide structures, enabling reconfigurable photonic components for the terahertz frequency range.
Production of Trehalose from Starch by Thermostable Enzymes from <i>Sulfolobus acidocaldarius</i> (1997)

Abstract The optimum conditions for the production of trehalose from starch were investigated using two thermostable enzymes, maltooligosyl trehalose synthase (MTSase) and maltooligosyl trehalose trehalohydrolase (MTHase), from Sulfolobus acidocaldarius ATCC 33909. The optimum pH was 5.5 and the optimum temperature was 55—57°C using isoamylase from Pseudomonas amyloderamosa as a debranching enzyme. The addition of CGTase to the reaction mixture during the saccharification process caused an increase in trehalose and a decrease in maltose and maltotriose. Isoamylase was better than pullulanase as a debranching enzyme. The yield of trehalose was independent of the type of starch used. Under optimum conditions, the yield of trehalose from corn starch at 30% concentration was more than 82%.
Inhibition of Cytomegalovirus Replication by Smooth-Muscle Relaxing Agents (1987)

The effect of the smooth-muscle relaxing agents, papaverine, sodium nitroprusside, and verapamil, on the replication of human cytomegalovirus (CMV) was investigated. At a concentration of 100 microM, infectious yields of CMV were reduced by 1.23 to 5.72 log10 by these drugs (papaverine, 5.72 log10; nitroprusside, 1.85 log10; verapamil, 1.23 log10). The ED50 for papaverine was found to be somewhat less than 1 microM, a concentration which appears to be within the range achieved clinically. Papaverine did not irreversibly modify treated cells to a virus-resistant state since treatment of cells with papaverine from 24 hr before until immediately prior to CMV infection did not significantly reduce CMV yields. Replication of CMV was most sensitive to inhibition when papaverine was added at or before 6 hr after CMV infection. Addition of papaverine at later times resulted in a substantial reduction of the inhibitory effect on virus yields, suggesting that the phase of CMV replication sensitive to papaverine inhibition occurred early in the replication cycle. These results, particularly in light of the potency of papaverine, indicate that some smooth-muscle relaxing agents have significant antiviral activity toward the replication of CMV.
Luneburg Lens for Wide-Angle Chip-Scale Optical Beam Steering (2019)

We present a design for a planar generalized Luneburg lens for use in a chip-scale optical beam steering device. The device has a theoretical in-plane field of view of 160° with no off-axis aberrations.