gebhart/patrepeval huggingface dataset:
Adaptable Hydrogels Mediate Cofactor‐Assisted Activation of Biomarker‐Responsive Drug Delivery via Positive Feedback for Enhanced Tissue Regeneration (2018)

The targeted and simultaneous delivery of diverse cargoes with vastly different properties by the same vehicle is highly appealing but challenging. Here, a bioactive nanocomposite hydrogel based on hyaluronic acid and self-assembled pamidronate-magnesium nanoparticles for the localized elution and on-demand simultaneous release of bioactive ions and small molecule drugs is described. The obtained nanocomposite hydrogels exhibit excellent injectability and efficient stress relaxation, thereby allowing easy injection and consequent adaptation of hydrogels to bone defects with irregular shapes. Magnesium ions released from the hydrogels promote osteogenic differentiation of the encapsulated human mesenchymal stem cells (hMSCs) and activation of alkaline phosphatase (ALP). The activated ALP subsequently catalyzes the dephosphorylation (activation) of Dex phosphate, a pro-drug of Dex, and expedites the release of Dex from hydrogels to further promote hMSC osteogenesis. This positive feedback circuit governing the activation and release of Dex significantly enhances bone regeneration at the hydrogel implantation sites. The findings suggest that these injectable nanocomposite hydrogels mediate optimized release of diverse therapeutic cargoes and effectively promote in situ bone regeneration via minimally invasive procedures.
Effect of hydrogen gas content in sintering atmosphere on the amount of free graphite in sintered steel made of iron powder containing sulfur, copper powder and graphite powder. (1998)

The solute carbon content and the ratio of pearlite in the sintered steel increased and the free graphite content and the sulfur content in the sintered steel decreased with the increase in hydrogen content in the sintered atmosphere during the sintering of green compact made of the iron powder containing sulfur, copper powder and graphite powder, resulting in the deterioration of machinability of the sintered steel.The amount of decrease in sulfur content during sintering was roughly the same as that calculated, on the assumption of hydrogen reduction of FeS in an equilibrium state.The Auger spectrum of the iron powder containing sulfur indicated that FeS segregated on the surface of the iron powder. The dilatometry study indicated that the carbon diffusing rate during sintering green compact containing iron powder with sulfur was smaller than that during sintering green compact containing commercially pure iron powder.These results showed that the free graphite was formed by incomplete carbon diffusion during sintering due to FeS on the surface of the iron powder with sulfur.
The role of polarization on surface plasmon polariton excitation on metallic gratings in the conical mounting (2010)

The polarization effects of surface plasmon polaritons (SPP) in an azimuthally rotated gratings have been investigated. Mixed s and p modes can be excited using the polarization angle of the incident light to optimize the SPP coupling. Experimental results and simulations show which component of polarization is effective for the SPP excitation. The optimum angle direction has been calculated analytically. The SPR tunability by polarization may uncover important features in SPP applications.
A novel cone beam breast CT scanner: preliminary system evaluation (2006)

The clinical goal of breast imaging is to detect tumor masses when they are as small as possible, preferably less than 10 mm in diameter. Conventional screen-film mammography is the most effective tool for the early detection of breast cancer currently available. However, conventional mammography has relatively low sensitivity for the detection of small breast cancers (under several millimeters). Specificity and the positive predictive value of mammography remain limited owing to an overlap in the appearance of benign and malignant lesions, and surrounding structure. We propose to address the limitations accompanying conventional mammography by incorporating a cone beam CT reconstruction technique with a recently developed flat panel detector (FPD). We have performed a computer simulation study and preliminary phantom studies to prove the feasibility of developing an FPD-based cone beam CT breast imaging technique for a small size normal breast phantom. In this study, we report the design and construction of a novel FPD-based cone beam breast CT scanner prototype. In addition, we present the results of phantom studies performed on our current FPD-based cone beam CT scanner prototype, which uses the same flat panel detector proposed for the cone beam breast CT scanner prototype, to predict the image performance of the novel cone beam breast CT scanner, while we are completing the construction of the system.
New rectifying semiconductor structure by molecular beam epitaxy (1980)

A new unipolar rectifying semiconductor structure is demonstrated. Rectification is produced by an asymmetric potential barrier created by an MBE-grown sawtooth-shaped composition wave of AlxGa1−xAs between layers of n-type GaAs. Single and multiple barriers as well as doped and undoped barriers have been studied and showed rectification. This is the first structure in which rectification has been produced directly by compositional grading.
A geometric approach to monitoring threshold functions over distributed data streams (2007)

Monitoring data streams in a distributed system is the focus of much research in recent years. Most of the proposed schemes, however, deal with monitoring simple aggregated values, such as the frequency of appearance of items in the streams. More involved challenges, such as the important task of feature selection (e.g., by monitoring the information gain of various features), still require very high communication overhead using naive, centralized algorithms. We present a novel geometric approach which reduces monitoring the value of a function (vis-à-vis a threshold) to a set of constraints applied locally on each of the streams. The constraints are used to locally filter out data increments that do not affect the monitoring outcome, thus avoiding unnecessary communication. As a result, our approach enables monitoring of arbitrary threshold functions over distributed data streams in an efficient manner. We present experimental results on real-world data which demonstrate that our algorithms are highly scalable, and considerably reduce communication load in comparison to centralized algorithms.
KChAP/Kvβ1.2 interactions and their effects on cardiac Kv channel expression (2001)

KChAP and voltage-dependent K+ (Kv) beta-subunits are two different types of cytoplasmic proteins that interact with Kv channels. KChAP acts as a chaperone for Kv2.1 and Kv4.3 channels. It also binds to Kv1.x channels but, with the exception of Kv1.3, does not increase Kv1.x currents. Kvbeta-subunits are assembled with Kv1.x channels; they exhibit "chaperone-like" behavior and change gating properties. In addition, KChAP and Kvbeta-subunits interact with each other. Here we examine the consequences of this interaction on Kv currents in Xenopus oocytes injected with different combinations of cRNAs, including Kvbeta1.2, KChAP, and either Kv1.4, Kv1.5, Kv2.1, or Kv4.3. We found that KChAP attenuated the depression of Kv1.5 currents produced by Kvbeta1.2, and Kvbeta1.2 eliminated the increase of Kv2.1 and Kv4.3 currents produced by KChAP. Both KChAP and Kvbeta1.2 are expressed in cardiomyocytes, where Kv1.5 and Kv2.1 produce sustained outward currents and Kv4.3 and Kv1.4 generate transient outward currents. Because they interact, either KChAP or Kvbeta1.2 may alter both sustained and transient cardiac Kv currents. The interaction of these two different classes of modulatory proteins may constitute a novel mechanism for regulating cardiac K+ currents.
Photonic bandgap VCSELs (2004)

A record-high output-power photonic-bandgap VCSEL is experimentally demonstrated. We employ nano-structuring in the VCSEL top mirror using a novel technique. Modeling and experimental results are in excellent agreement.
Quasistatic Cavity Resonance for Ubiquitous Wireless Power Transfer (2017)

Wireless power delivery has the potential to seamlessly power our electrical devices as easily as data is transmitted through the air. However, existing solutions are limited to near contact distances and do not provide the geometric freedom to enable automatic and un-aided charging. We introduce quasistatic cavity resonance (QSCR), which can enable purpose-built structures, such as cabinets, rooms, and warehouses, to generate quasistatic magnetic fields that safely deliver kilowatts of power to mobile receivers contained nearly anywhere within. A theoretical model of a quasistatic cavity resonator is derived, and field distributions along with power transfer efficiency are validated against measured results. An experimental demonstration shows that a 54 m3 QSCR room can deliver power to small coil receivers in nearly any position with 40% to 95% efficiency. Finally, a detailed safety analysis shows that up to 1900 watts can be transmitted to a coil receiver enabling safe and ubiquitous wireless power.
Oral supplementation of healthy adults with 2′-<i>O</i>-fucosyllactose and lacto-<i>N</i>-neotetraose is well tolerated and shifts the intestinal microbiota (2016)

The gut microbiota has been established as an important player influencing many aspects of human physiology. Breast milk, the first diet for an infant, contains human milk oligosaccharides (HMO) that shape the infant's gut microbiota by selectively stimulating the growth of specific bacteria, especially bifidobacteria. In addition to their bifidogenic activity, the ability of HMO to modulate immune function and the gut barrier makes them prime candidates to restore a beneficial microbiota in dysbiotic adults and provide health benefits. We conducted a parallel, double-blind, randomised, placebo-controlled, HMO-supplementation study in 100 healthy, adult volunteers, consuming chemically produced 2'-O-fucosyllactose (2'FL) and/or lacto-N-neotetraose (LNnT) at various daily doses and mixes or placebo for 2 weeks. All participants completed the study without premature discontinuation. Supplementation of 2'FL and LNnT at daily doses up to 20 g was shown to be safe and well tolerated, as assessed using the gastrointestinal symptoms rating scale. 16S rRNA sequencing analysis showed that HMO supplementation specifically modified the adult gut microbiota with the primary impact being substantial increases in relative abundance of Actinobacteria and Bifidobacterium in particular and a reduction in relative abundance of Firmicutes and Proteobacteria. This study provides the first set of data on safety, tolerance and impact of HMO on the adult gut microbiota. Collectively, the results from this study show that supplementing the diet with HMO is a valuable strategy to shape the human gut microbiota and specifically promote the growth of beneficial bifidobacteria.
Planar Retroreflector (2014)

We propose a planar retroreflector composed of two cascaded high contrast periodic structures with slowly varying features. One of the high contrast structures focuses the light while the other reflects it as a concave mirror.
Vertical channel field-controlled thyristors with high gain and fast switching speeds (1978)

A new, planar, surface-grid, field-controlled thyristor (FCT) structure is described. The structure is fabricated by using orientation-dependent (preferential) etching and selective vapor epitaxial growth to obtain vertical grid walls. The resulting high channel-length-to-width aspect ratio produces devices with high blocking gains and fast gate turnoff speeds. Devices have been fabricated with the capability of blocking more than 1000 V with an applied grid bias of 32 V, and simultaneously exhibiting a low forward voltage drop in the on-state. These surface-grid devices exhibit gate turnoff capability with turnoff times of less than 500 ns at a rated cathode-anode current of 1 A.
IES Transaction: Compact Lamp with two Interior Fluorescent Tubes (1984)

(1984). IES Transaction: Compact Lamp with two Interior Fluorescent Tubes. Journal of the Illuminating Engineering Society: Vol. 14, 1984 IES Annual Conference papers, pp. 298-305.
Controlled levitation of a large magnet above superconductors (1990)

The levitation of a permanent magnet over a type-II superconductor may be modified and controlled by the addition of a variable magnetic field to the magnet-superconductor system. Using this scheme, levitation of a magnet of significantly larger mass was established by the direct interaction of the additonal field with the levitating magnet.
Serodiagnosis of infectious mononucleosis with a bovine erythrocyte glycoprotein (1983)

A glycoprotein from bovine erythrocyte membrane was evaluated in two immunoassays as a reagent for the serodiagnosis of infectious mononucleosis (IM). We previously reported that a partially purified preparation of this glycoprotein, when attached to latex beads, agglutinated in the presence of IM heterophile antibody. In the present study, we used a highly purified form of the glycoprotein both as an agglutinating reagent, covalently bound to latex, and in a solid-phase sandwich-type radioimmunoassay (RIA) for IM antibody detection in a larger population of patients. We tested serum samples from college students with symptoms suggestive of IM with the latex reagent (143 samples) and with the RIA (245 samples). Correlation of these two tests, both with each other and with the classical differentially absorbed, agglutination tests for Paul-Bunnell antibody in IM sera, using fresh sheep or horse cells, was excellent (greater than 97% agreement). The new tests also corresponded in most cases with a rapid, unabsorbed preserved horse erythrocyte slide test. However, in this study of 245 samples, both apparent false-positives (5 samples) and apparent false-negatives (3 samples) were observed with this slide test. In conclusion, we found that the bovine glycoprotein as a reagent can facilitate the diagnosis of IM, giving results comparable to those with erythrocyte agglutination tests on differentially absorbed sera. The advantages are ease and speed of performance (latex test), potential for automation (RIA test), stability and uniformity of the glycoprotein reagent (latex and RIA tests), and most importantly, the ability to use unabsorbed sera (latex and RIA tests).
Novel hydrogen gas sensor based on single ZnO nanorod (2008)

For extensive use in an industrialized process of individual ZnO nano/microrods as building nanoblock in novel hydrogen sensors, a simple, inexpensive, and bio-safe synthesis process and nanofabrication route is required. Here, we report a cost-effective and fast synthesis route for ZnO one-dimensional nanorod using an aqueous-based approach in a reactor. Our synthesis technique permits nano/microrods to be easily transferred to other substrates and to be distributed on the surface. This flexibility of substrate choice opens the possibility of using focused ion beam (FIB/SEM) system for handling and fabricating nanosensors. The main advantage of this procedure is a quick verification/testing of concept and is compatible with micro/nanoelectronic devices. The described nanofabrication steps permitted us to obtain a 90% success rate for building single nanorod sensor. A sensitivity of ∼4% was obtained for a single ZnO nanorod hydrogen sensor at 200 ppm H2 in the air at room temperature. The nanosensor has a high selectivity for H2, since its sensitivity for O2, CH4, CO, ethanol or LPG are less than 0.25%. © 2008 Elsevier B.V. All rights reserved.
Nanometer‐scale imaging by the modulation tracking method (2011)

We developed an optical imaging method based on a feedback principle in which the specific scan pattern is adapted according to the shape of the sample. The feedback approach produces nanometer-resolved 3D images of very small and moving features in live cells in seconds. We show images of microvilli in live cultured opossum kidney cells expressing NaPi co-transporter proteins with different GFP constructs and images of cell protrusions in a collagen matrix with a resolution of about 20 nm. We found that in the microvilli the NaPi proteins can be found clustered. Along cell protrusions in 3D we identified cellular adhesions to the extracellular matrix. Our approach to super-resolution and to 3D nanoimaging is different than other proposed methods that break the diffraction limit using non-linear effects or are based on single molecule localization.
A feasibility study for predicting optimal radiation therapy dose distributions of prostate cancer patients from patient anatomy using deep learning (2019)

With the advancement of treatment modalities in radiation therapy for cancer patients, outcomes have improved, but at the cost of increased treatment plan complexity and planning time. The accurate prediction of dose distributions would alleviate this issue by guiding clinical plan optimization to save time and maintain high quality plans. We have modified a convolutional deep network model, U-net (originally designed for segmentation purposes), for predicting dose from patient image contours of the planning target volume (PTV) and organs at risk (OAR). We show that, as an example, we are able to accurately predict the dose of intensity-modulated radiation therapy (IMRT) for prostate cancer patients, where the average Dice similarity coefficient is 0.91 when comparing the predicted vs. true isodose volumes between 0% and 100% of the prescription dose. The average value of the absolute differences in [max, mean] dose is found to be under 5% of the prescription dose, specifically for each structure is [1.80%, 1.03%](PTV), [1.94%, 4.22%](Bladder), [1.80%, 0.48%](Body), [3.87%, 1.79%](L Femoral Head), [5.07%, 2.55%](R Femoral Head), and [1.26%, 1.62%](Rectum) of the prescription dose. We thus managed to map a desired radiation dose distribution from a patient's PTV and OAR contours. As an additional advantage, relatively little data was used in the techniques and models described in this paper.
The CspA family in <i>Escherichia coli</i> : multiple gene duplication for stress adaptation (1998)

CspA was originally found as the major cold-shock protein in Escherichia coli, consisting of 70-amino-acid residues. It forms a beta-barrel structure with five anti-parallel beta-strands and functions as an RNA chaperone. Its dramatic but transient induction upon cold shock is regulated at the level of transcription, mRNA stability and translation. Surprisingly, E. coli contains a large CspA family, consisting of nine genes from cspA to cspI. Phylogenetic analysis of these gene products and the cold-shock domain of human YB-1 protein reveals that there are two major branches in the evolution of CspA homologues: one branch for CspF and CspH, and another for all the other known CspA homologues from both prokaryotes and eukaryotes. The locations of these genes on the E. coli chromosome suggest that the large CspA family probably resulted from a number of gene duplications and, after subsequent adaptation, resulted in specific groups of genes that respond to different environmental stresses; for example, cspA, cspB and cspG for cold-shock stress and cspD for nutritional deprivation. The E. coli CspA family will be discussed in terms of their structures and functions, and their gene structures and regulation.
Novel Antibody Drug Conjugates Targeting Tumor-Associated Receptor Tyrosine Kinase ROR2 by Functional Screening of Fully Human Antibody Libraries Using Transpo-mAb Display on Progenitor B Cells (2018)

Receptor tyrosine kinase-like orphan receptor 2 (ROR2) has been identified as a highly relevant tumor-associated antigen in a variety of cancer indications of high unmet medical need, including renal cell carcinoma and osteosarcoma, making it an attractive target for targeted cancer therapy. Here, we describe the <i>de novo</i> discovery of fully human ROR2-specific antibodies and potent antibody drug conjugates (ADCs) derived thereof by combining antibody discovery from immune libraries of human immunoglobulin transgenic animals using the Transpo-mAb mammalian cell-based IgG display platform with functional screening for internalizing antibodies using a secondary ADC assay. The discovery strategy entailed immunization of transgenic mice with the cancer antigen ROR2, harboring transgenic IgH and IgL chain gene loci with limited number of fully human V, D, and J gene segments. This was followed by recovering antibody repertoires from the immunized animals, expressing and screening them as full-length human IgG libraries by transposon-mediated display in progenitor B lymphocytes ("Transpo-mAb Display") for ROR2 binding. Individual cellular "Transpo-mAb" clones isolated by single cell sorting and capable of expressing membrane-bound as well as secreted human IgG were directly screened during antibody discovery, not only for high affinity binding to human ROR2, but also functionally as ADCs using a cytotoxicity assay with a secondary anti-human IgG-toxin-conjugate. Using this strategy, we identified and validated 12 fully human, monoclonal anti-human ROR2 antibodies with nanomolar affinities that are highly potent as ADCs and could be promising candidates for the therapy of human cancer. The screening for functional and internalizing antibodies during the early phase of antibody discovery demonstrates the utility of the mammalian cell-based Transpo-mAb Display platform to select for functional binders and as a powerful tool to improve the efficiency for the development of therapeutically relevant ADCs.
Modular small-molecule directed nanoparticle assembly (2010)

We demonstrate a simple and versatile approach to produce hierarchical assemblies of nanoparticles by combining block copolymers and small molecules. Directed nanoparticle assemblies can be achieved with a variety of nanoparticles and small molecules without modification of the nanoparticle ligands. This novel approach opens up new routes toward the fabrication of nanoparticle-based functional devices using a “Bottom-Up” approach.
Update on endodontic irrigating solutions (2012)

Bacteria have long been recognized as the primary etiological factor in the development of pulp and periapical lesions. Successful root canal therapy depends on thorough debridement of pulpal tissue, dentin debris, and infective microorganisms. Currently, it is impossible to eradicate intraradicular infection with mechanical instrumentation alone. Therefore, irrigants are required to complete this task. In this article, the different actions and interactions of the most commonly used irrigants are discussed. The aim of this review is to analyze the relevant literature on root canal irrigants.
Active sites of ligand-protected Au25 nanoparticle catalysts for CO2 electroreduction to CO (2016)

Recent experimental studies have reported the electrochemical reduction of carbon dioxide (CO2) into CO at atomically precise negatively charged Au25 (-) nanoclusters. The studies showed CO2 conversion at remarkably low overpotentials, but the exact mechanisms and nature of the active sites remain unclear. We used first-principles density functional theory and continuum solvation models to examine the role of the cluster during electrochemical CO2 reduction and analyze the free energies of proposed intermediate species. Contrary to previous assumptions, our results show that the fully ligand protected cluster is not an active CO2 reduction catalyst because formation of the crucial carboxyl intermediate required very high electrochemical potentials. Instead, our calculations suggest that the reduction process likely occurs on a dethiolated gold site, and adsorbed carboxyl intermediate formation was significantly stabilized at dethiolated gold sites. These findings point to the crucial role of exposed metal sites during electrochemical CO2 reduction at gold nanocluster catalysts.