gebhart/patrepeval huggingface dataset:
Gd‐Encapsulated Carbonaceous Dots with Efficient Renal Clearance for Magnetic Resonance Imaging (2014)

Nanoprobes for MRI and optical imaging are demonstrated. Gd@C-dots possess strong fluorescence and can effectively enhance signals on T1 -weighted MR images. The nanoprobes have low toxicity, and, despite a relatively large size, can be efficiently excreted by renal clearance from the host after systemic injection.
Simultaneous multiple view high resolution surface geometry acquisition using structured light and mirrors (2013)

Knowledge of the surface geometry of an imaging subject is important in many applications. This information can be obtained via a number of different techniques, including time of flight imaging, photogrammetry, and fringe projection profilometry. Existing systems may have restrictions on instrument geometry, require expensive optics, or require moving parts in order to image the full surface of the subject. An inexpensive generalised fringe projection profilometry system is proposed that can account for arbitrarily placed components and use mirrors to expand the field of view. It simultaneously acquires multiple views of an imaging subject, producing a cloud of points that lie on its surface, which can then be processed to form a three dimensional model. A prototype of this system was integrated into an existing Diffuse Optical Tomography and Bioluminescence Tomography small animal imaging system and used to image objects including a mouse-shaped plastic phantom, a mouse cadaver, and a coin. A surface mesh generated from surface capture data of the mouse-shaped plastic phantom was compared with ideal surface points provided by the phantom manufacturer, and 50% of points were found to lie within 0.1mm of the surface mesh, 82% of points were found to lie within 0.2mm of the surface mesh, and 96% of points were found to lie within 0.4mm of the surface mesh.
A LABORATORY-BASED X-RAY PHASE CONTRAST IMAGING SCANNER WITH APPLICATIONS IN BIOMEDICAL AND NON-MEDICAL DISCIPLINES (2014)

X-ray phase contrast imaging (XPCi) provides a much higher visibility of low-absorbing details than conventional, attenuation-based radiography. This is due to the fact that image contrast is determined by the unit decrement of the real part of the complex refractive index of an object rather than by its imaginary part (the absorption coefficient), which can be up to 1000 times larger for energies in the X-ray regime. This finds applications in many areas, including medicine, biology, material testing, and homeland security. Until lately, XPCi has been restricted to synchrotron facilities due to its demanding coherence requirements on the radiation source. However, edge illumination XPCi, first developed by one of the authors at the ELETTRA Synchrotron in Italy, substantially relaxes these requirements and therefore provides options to overcome this problem. Our group has built a prototype scanner that adapts the edge-illumination concept to standard laboratory conditions and extends it to large fields of view. This is based on X-ray sources and detectors available off the shelf, and its use has led to impressive results in mammography, cartilage imaging, testing of composite materials and security inspection. This article presents the method and the scanner prototype, and reviews its applications in selected biomedical and non-medical disciplines.
Cloning of RNA molecules<i>in vitro</i> (1993)

A method for RNA amplification in an immobilized medium is described. The medium contains a complete set of nucleotide substrates and purified Q beta replicase, an enzyme capable of exponentially amplifying RNAs under isothermal conditions. RNA amplification in the immobilized medium results in the formation of separate 'colonies', each comprising the progeny of a single RNA molecule (a clone). The colonies were visualized by staining with ethidium bromide, by utilizing radioactive substrates, and by hybridization with sequence-specific labeled probes. The number and identity of the RNA colonies corresponded to that of the RNAs seeded. When a mixture of different RNA species was seeded, these species were found in different colonies. Possible implementations of this technique include a search for recombinant RNAs, very sensitive nucleic acid diagnostics, and gene cloning in vitro.
Perspectives on advances in high-capacity, free-space communications using multiplexing of orbital-angular-momentum beams (2021)

Beams carrying orbital-angular-momentum (OAM) have gained much interest due to their unique amplitude and phase structures. In terms of communication systems, each of the multiple independent data-carrying beams can have a different OAM value and be orthogonal to all other beams. This paper will describe the use of multiplexing and the simultaneous transmission of multiple OAM beams for enhancing the capacity of communication systems. We will highlight the key advances and technical challenges in the areas of (a) free-space and fiber communication links, (b) mitigation of modal coupling and channel crosstalk effects, (c) classical and quantum systems, and (d) optical and radio frequency beam multiplexing.
Electroporation followed by electrochemical measurement of quantal transmitter release from single cells using a patterned microelectrode (2013)

An electrochemical microelectrode located immediately adjacent to a single neuroendocrine cell can record spikes of amperometric current that result from exocytosis of oxidizable transmitter from individual vesicles, i.e., quantal exocytosis. Here, we report the development of an efficient method where the same electrochemical microelectrode is used to electropermeabilize an adjacent chromaffin cell and then measure the consequent quantal catecholamine release using amperometry. Trains of voltage pulses, 5-7 V in amplitude and 0.1-0.2 ms in duration, were used to reliably trigger release from cells using gold electrodes. Amperometric spikes induced by electropermeabilization had similar areas, peak heights and durations as amperometric spikes elicited by depolarizing high K(+) solutions, therefore release occurs from individual secretory granules. Uptake of trypan blue stain into cells demonstrated that the plasma membrane is permeabilized by the voltage stimulus. Voltage pulses did not degrade the electrochemical sensitivity of the electrodes assayed using a test analyte. Surprisingly, robust quantal release was elicited upon electroporation in the absence of Ca(2+) in the bath solution (0 Ca(2+)/5 mM EGTA). In contrast, electropermeabilization-induced transmitter release required Cl(-) in the bath solution in that bracketed experiments demonstrated a steep dependence of the rate of electropermeabilization-induced transmitter release on [Cl(-)] between 2 and 32 mM. Using the same electrochemical electrode to electroporate and record quantal release of catecholamines from an individual chromaffin cell allows precise timing of the stimulus, stimulation of a single cell at a time, and can be used to load membrane-impermeant substances into a cell.
Can the complications of distal locking be prevented with a new nail that offers a novel locking technique in the treatment of humeral shaft fractures? (2020)

InSafeLOCK® humeral nail is safe when applied with the recommended technique. It can easily be applied without damaging the veins, nerves or other soft tissues around the elbow due to the internal distal locking feature; furthermore, there is no need to use fluoroscopy or targeting guide. Thus, it is possible to avoid complications that may occur during and after distal locking in conventional intramedullary nail implementations.
Contactless in ovo sex determination of chicken eggs (2017)

Abstract We resume the research for contactless in ovo sex determination of the domestic chicken, which has the final aim to provide an alternative to day-old cockerels culling in the layer industry. In ovo Raman and fluorescence spectroscopy of blood of eggs incubated until day 3.5 enables correct sexing rates over 90% barely affecting the hatching rate. Full automatization of the processes to guarantee high sexing speed and fulfill industrial demands is required to allow transferring the technology inside the hatcheries in the next future.
Cone-beam volume CT mammographic imaging: feasibility study (2001)

X-ray projection mammography, using a film/screen combination or digital techniques, has proven to be the most effective imaging modality for early detection of breast cancer currently available. However, the inherent superimposition of structures makes small carcinoma (a few millimeters in size) difficult to detect in the occultation case or in dense breasts, resulting in a high false positive biopsy rate. The cone-beam x-ray projection based volume imaging using flat panel detectors (FPDs) makes it possible to obtain three-dimensional breast images. This may benefit diagnosis of the structure and pattern of the lesion while eliminating hard compression of the breast. This paper presents a novel cone-beam volume CT mammographic imaging protocol based on the above techniques. Through computer simulation, the key issues of the system and imaging techniques, including the x-ray imaging geometry and corresponding reconstruction algorithms, x-ray characteristics of breast tissues, x-ray setting techniques, the absorbed dose estimation and the quantitative effect of x-ray scattering on image quality, are addressed. The preliminary simulation results support the proposed cone-beam volume CT mammographic imaging modality in respect to feasibility and practicability for mammography. The absorbed dose level is comparable to that of current two-view mammography and would not be a prominent problem for this imaging protocol. Compared to traditional mammography, the proposed imaging protocol with isotropic spatial resolution will potentially provide significantly better low contrast detectability of breast tumors and more accurate location of breast lesions.
Optimal frequency of walsh mask broadcast for forward high-speed wireless packet data channels (2005)

The 1/spl times/EV-DV 3G wireless network system is able to simultaneously support traditional code division multiplexed voice and circuit-switched data channels in addition to one or two time shared high speed packet data channels. In practice, voice calls will be provided with higher priority than data calls. Therefore, radio resources (e.g., power and Walsh codes) will be first given to voice connections and any unused resources will be dynamically assigned to the high speed packet data channel. Because of Walsh code fragmentation, it may be possible that Walsh codes not being used for voice and circuit-switched data connections cannot be used by the packet data channel. In order to make use of such codes, the Walsh code mask must be broadcast in the sector. Such a broadcast results in increased sector throughput due to the additional Walsh codes available to the forward packet data channel (F-PDCH) but it also requires forward link resources which reduces throughput. We determine the broadcast frequency that optimizes this tradeoff.
CHET: an optimizing compiler for fully-homomorphic neural-network inferencing (2019)

Fully Homomorphic Encryption (FHE) refers to a set of encryption schemes that allow computations on encrypted data without requiring a secret key. Recent cryptographic advances have pushed FHE into the realm of practical applications. However, programming these applications remains a huge challenge, as it requires cryptographic domain expertise to ensure correctness, security, and performance.
Simvastatin offers new prospects for the treatment of Duchenne muscular dystrophy (2016)

Duchenne muscular dystrophy (DMD) is the most common and severe inherited neuromuscular disorder. DMD is caused by mutations in the gene encoding the dystrophin protein in muscle fibers. Dystrophin was originally proposed to be a structural protein that protected the sarcolemma from stresses produced during contractions. However, more recently, experimental evidence has revealed a far more complicated picture, with the loss of dystrophin causing dysfunction of multiple muscle signaling pathways, which all contribute to the overall disease pathophysiology. Current gene-based approaches for DMD are conceptually appealing since they offer the potential to restore dystrophin to muscles, albeit a partially functional, truncated form of the protein. However, given the cost and technical challenges facing these genetic approaches, it is important to consider if relatively inexpensive, clinically used drugs may be repurposed for treating DMD. Here, we discuss our recent findings showing the potential of simvastatin as a novel therapy for DMD.
Membrane-Disrupting Nanofibrous Peptide Hydrogels (2019)

Self-assembled peptide nanofibers can form biomimetic hydrogels at physiological pH and ionic strength through noncovalent and reversible interactions. Inspired by natural antimicrobial peptides, we designed a class of cationic amphiphilic self-assembled peptides (CASPs) that self-assemble into thixotropic nanofibrous hydrogels. These constructs employ amphiphilicity and high terminal charge density to disrupt bacterial membranes. Here, we focus on three aspects of the self-assembly of these hydrogels: (a) the material properties of the individual self-assembled nanofibers, (b) emergence of bulk-scale elasticity in the nanofibrous hydrogel, and (c) trade-off between the desirable material properties and antimicrobial efficacy. The design of the supramolecular nanofibers allows for higher-order noncovalent ionic cross-linking of the nanofibers into a viscoelastic network. We determine the stiffness of the self-assembled nanofibers via the peak force quantitative nanomechanical atomic force microscopy and the bulk-scale rheometry. The storage moduli depend on peptide concentration, ionic strength, and concentration of multivalent ionic cross-linker. CASP nanofibers are demonstrated to be effective against <i>Pseudomonas aeruginosa</i> colonies. We use nanomechanical analysis and microsecond-time scale coarse-grained simulation to elucidate the interaction between the peptides and bacterial membranes. We demonstrate that the membranes stiffen, contract, and buckle after binding to peptide nanofibers, allowing disruption of osmotic equilibrium between the intracellular and extracellular matrix. This is further associated with dramatic changes in cell morphology. Our studies suggest that self-assembled peptide nanofibrils can potentially acts as membrane-disrupting antimicrobial agents, which can be formulated as injectable hydrogels with tunable material properties.
Optimization of <scp>L</scp>‐phenylalanine production of <i>Corynebacterium glutamicum</i> under product feedback inhibition by elevated oxygen transfer rate (2001)

Production feedback inhibition both on cell growth and on product formation of phenylalanine fermentation might be alleviated by elevated oxygen supply. Batch fermentations by a high phenylalanine producing strain Corynebacterium glutamicum CCRC 18335 at various initial phenylalanine concentrations (P(0)) ranging from 0 to 20 g/L and different oxygen transfer rate coefficients (K(L)a) ranging from 23 to 76 h(-1) were studied. The fermentation parameters with respect to P(0) were strongly dependent on K(L)a. Cell yield favored higher K(L)a and lower P(0). Product yield with respect to varying phenylalanine concentration was evaluated by the relative oxygen availability (ROA). The optimal ROA for phenylalanine formation was strongly dependent on the product concentration. While P(0) was low, the product inhibition was less significant and the maximum product yield occurred while ROA was at 0.5-0.6. While P(0) was high, the product inhibition was significant and the maximum product yield occurred while ROA was at 0.8-0.9. These results suggest that the product feedback inhibition of phenylalanine fermentation processes can be alleviated by a gradual increase in oxygen supply rate while the increasing product concentration is taken into account. The strategy is demonstrated in a fed-batch culture with elevated oxygen supply. The final phenylalanine concentration was 23.2 g/L, which was 45% better than that of the fed-batch fermentation without elevated oxygen supply. Likewise, the maximum productivity was improved by 42% at 0.37 g/(L x h).
Network-Centric Quantum Communications (2013)

Network-centric quantum communications (NQC) - a new, scalable instantiation of quantum cryptography providing key management with forward security for lightweight encryption, authentication and digital signatures in optical networks – is briefly described.
Design of Flexible Wing for Flapping Flight by Fluid-Structure Interaction Analysis (2006)

We investigated some configurations of middle-size and high-aspect ratio wings for flapping flight by fluid-structure interaction simulation. Mimicking the flapping flight of insects is a useful method of microrobot motion. Its comprehensive unsteady aerodynamics has gradually been unraveled and several types of flapping-flight robots have been proposed. We examined the motion of a dragonfly, and try to apply it to microrobots. In designing such nimble flapping flight, adequate evaluation of the deformation of the wing is unavoidable. Wings of such species have high aspect ratio because a small momentum of inertia around the longitudinal axis is a great advantage in controlling. Such a slender wing is easy to twist around the longitudinal direction. Thus, in order to design such a wing, the wing behavior caused by interaction with the airflow must be analyzed, and the adequate stiffness must be determined. We designed two types of wings based on the architecture of the dragonfly’s wing, and examined the performance of the wings by fluid-structure interaction analysis. Here we show some examples of the designs and the performance of the wings for hovering, as the results of the first trial.
Effect of H<sub>2</sub>S on the Performance and Long-Term Stability of Pd/Cu Membranes (2009)

The objective of this work was to fabricate palladium membranes with a Pd/Cu alloy top layer and to investigate the performance and long-term stability of the membranes in small concentrations of H2S. The Pd/Cu alloy membranes with compositions of 8, 18, and 19 wt % copper were fabricated using the electroless deposition method on porous Inconel supports and characterized for several thousand hours in H2, helium, and 45−55 ppm H2S/H2 mixtures in the temperature range of 350−500 °C. Upon exposure to the H2S/H2 mixture at 500 °C, the Pd/Cu membranes lost ∼80% of the hydrogen permeance, because of the H2S forming surface sulfides and blocking H2 adsorption sites, with the amount of permeance lost increasing with decreasing temperature. Reintroducing pure H2 recovered some of the hydrogen permeance, showing that some of the H2S poisoning was irreversible. The amount of irreversible poisoning increased as the H2S exposure time increased and the exposure temperature decreased, because of the exothermic nature of H2S adsorption. The helium leak rate of the Pd/Cu membranes decreased after the poisoning experiments, possibly because of sulfur that segregated to the grain boundaries of the Pd/Cu deposits.
Preventing PCM banks from seizing too much power (2011)

Widespread adoption of Phase Change Memory (PCM) requires solutions to several problems recently addressed in the literature, including limited endurance, increased write latencies, and system-level changes required to exploit non-volatility. One important difference between PCM and DRAM that has received less attention is the increased need for write power management. Writing to a PCM cell requires high current density over hundreds of nanoseconds, and hard limits on the number of simultaneous writes must be enforced to ensure correct operation, limiting write throughput and therefore overall performance. Because several wear reduction schemes only write those bits that need to be written, the amount of power required to write a cache line back to memory under such a system is now variable, which creates opportunity to reduce write power. This paper proposes policies that monitor the bits that have actually been changed over time, as opposed to simply those lines that are dirty. These polices can more effectively allocate power across the system to improve write concurrency. This method for allocating power across the memory subsystem is built on the idea of "power tokens," a transferable, but time-specific, allocation of power. The results show that with a storage overhead of 4.3% in the last-level cache, a power-aware memory system can improve the performance of multiprogrammed workloads by up to 84%.
Conditional interpolation: making concurrent program verification more effective (2021)

Due to the state-space explosion problem, efficient verification of real-world programs in large scale is still a big challenge. Particularly, thread alternation makes the verification of concurrent programs much more difficult since it aggravates this problem. In this paper, an application of Craig interpolation, namely conditional interpolation, is proposed to work together with CEGAR-based approach to reduce the state-space of concurrent tasks. Specifically, conditional interpolation is formalized to confine the reachable region of states so that infeasible conditional branches could be pruned. Furthermore, the generated conditional interpolants are utilized to shorten the interpolation paths, which makes the time consumed for verification significantly reduced. We have implemented the proposed approach on top of an open-source software model checker. Empirical results show that the conditional interpolation is effective in improving the verification efficiency of concurrent tasks.
Hydrothermal Syntheses and Structural Characterization of Layered Vanadium Oxides Incorporating Organic Cations:  α-, β-(H<sub>3</sub>N(CH<sub>2</sub>)<sub>2</sub>NH<sub>3</sub>)[V<sub>4</sub>O<sub>10</sub>] and α-, β-(H<sub>2</sub>N(C<sub>2</sub>H<sub>4</sub>)<sub>2</sub>NH<sub>2</sub>)[V<sub>4</sub>O<sub>10</sub>] (1996)

Four new layered mixed-valence vanadium oxides, which contain interlamellar organic cations, alpha-(H(3)N(CH(2))(2)NH(3))[V(4)O(10)] (1a), beta-(H(3)N(CH(2))(2)NH(3))[V(4)O(10)] (1b), alpha-(H(2)N(C(2)H(4))(2)NH(2))[V(4)O(10)] (2a), and beta-(H(2)N(C(2)H(4))(2)NH(2))[V(4)O(10)] (2b), have been prepared under hydrothermal conditions and their single-crystal structures determined: 1a, triclinic, space group P&onemacr;, a = 6.602(2) Å, b = 7.638(2) Å, c = 5.984(2) Å, alpha = 109.55(3) degrees, beta = 104.749(2) degrees, gamma = 82.31(3) degrees, Z = 1; 1b, triclinic, P&onemacr;, a = 6.387(1) Å, b = 7.456(2) Å, c = 6.244(2) Å, alpha = 99.89(2) degrees, beta = 102.91(2) degrees, gamma = 78.74(2) degrees, Z = 1; 2a, triclinic, P&onemacr;, a = 6.3958(5) Å, b = 8.182(1) Å, c = 6.3715(7) Å, alpha = 105.913(9) degrees, beta = 104.030(8) degrees, gamma = 94.495(8) degrees, Z = 1; 2b, monoclinic, space group P2(1)/n, a = 9.360(2) Å, b = 6.425(3) Å, c = 10.391(2) Å, beta = 105.83(1) degrees, Z = 2. All four of the compounds contain mixed-valence V(5+)/V(4+) vanadium oxide layers constructed from V(5+)O(4) tetrahedra and pairs of edge-sharing V(4+)O(5) square pyramids with protonated organic amines occupying the interlayer space.
Use of a Fiber Optic Probe for the Detection and Identification of Explosive Materials by Raman Spectroscopy (1995)

Abstract A fiber optic probe has been developed that allows Raman spectra to be acquired remotely from a spectrometer. This system has been used to acquire spectra from explosive materials. In this study we demonstrate that it is sufficiently sensitive to rapidly detect traces of PETN in fingerprints of Semtex-Hexplosive. Further development of similar systems will enable the detection and non-destructive identification of contraband materials in the field.