gebhart/patrepeval huggingface dataset:
A Comparative Study Between Spray-Drying and Fluidized Bed Coating Processes for the Preparation of Pramipexole Controlled-Release Microparticles for Orally Disintegrating Tablets (2014)

In the present study, controlled-release microparticles for orally disintegrating tablets (ODT) were prepared using two different processes, spray drying and fluidized bed coating processes. Pramipexole dihydrochloride monohydrate (PRM), an anti-Parkinson's disease agent, was selected as a model drug. The in vitro release rate and morphology of microparticles were evaluated and compared. The size of microparticles prepared by spray drying (SD microparticles) and fluidized bed coating (FC microparticles) was around 10 and 200 µm, respectively. The latter size was defined by the size of an inert core bead. The release behavior of SD microparticles was characterized by a large initial burst release prior to slow release. In the case of FC microparticles, the initial burst release was smaller than that of SD microparticles and the compression process damaged the release-controlling layer, which led to a change in release rate. The results indicated the importance of carefully considering the manufacturing process for microparticles during the design of controlled-release ODT.
Antibacterial and antifungal efficacy of surface functionalized polymeric beads in repeated applications (2004)

A simple method was developed to prepare polymeric microbeads with antibacterial and antifungal properties. The microbeads of approximately spherical shape and narrow size distribution were prepared from a mixture of poly (4-vinyl pyridine) (P4VP) and poly (vinylidene fluoride) (PVDF) by a phase inversion technique and subsequently derivatized with alkyl bromides having 4-10 carbon atoms. The quaternization of the pyridine groups into pyridinium groups confers the surface with highly effective and long-lasting antibacterial and antifungal properties, as shown by the effect on Escherichia coli and Aspergillus niger. Upon contact with the N-alkylated beads, the bacteria and fungal spores are lysed and intracellular constituents leach out into the medium. The efficacy of the alkyl chains in disrupting the cell membrane was investigated. The stability of the functional group and microbiocidal effectiveness of the microbeads in repeated applications was also assessed.
Characterization of monoclonal ZnT8-specific antibody (2013)

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Architectural support for uniprocessor and multiprocessor active memory systems (2004)

We introduce an architectural approach to improve memory system performance in both uniprocessor and multiprocessor systems. The architectural innovation is a flexible active memory controller backed by specialized cache coherence protocols that permit the transparent use of address remapping techniques. The resulting system shows significant performance improvement across a spectrum of machine configurations, from uniprocessors through single-node multiprocessors (SMPs) to distributed shared memory clusters (DSMs). Address remapping techniques exploit the data access patterns of applications to enhance their cache performance. However, they create coherence problems since the processor is allowed to refer to the same data via more than one address. While most active memory implementations require cache flushes, we present a new approach to solve the coherence problem. We leverage and extend the cache coherence protocol so that our techniques work transparently to efficiently support uniprocessor, SMP and DSM active memory systems. We detail the coherence protocol extensions to support our active memory techniques and present simulation results that show uniprocessor speedup from 1.3 to 7.6 on a range of applications and microbenchmarks. We also show remarkable performance improvement on small to medium-scale SMP and DSM multiprocessors, allowing some parallel applications to continue to scale long after their performance levels off on normal systems.
Design and Synthesis of a New [<sup>18</sup>F]Fluoropyridine-Based Haloacetamide Reagent for the Labeling of Oligonucleotides:  2-Bromo-<i>N</i>-[3-(2-[<sup>18</sup>F]fluoropyridin-3-yloxy)propyl]acetamide (2004)

Based on the recently highlighted potential of nucleophilic heteroaromatic ortho-radiofluorinations in the preparation of fluorine-18-labeled radiotracers and radiopharmaceuticals for PET, a [(18)F]fluoropyridine-based bromoacetamide reagent has been prepared and used in prosthetic group introduction for the labeling of oligonucleotides. [(18)F]FPyBrA (2-bromo-N-[3-(2-[(18)F]fluoropyridin-3-yloxy)propyl]acetamide) was designed as a radiochemically feasible reagent, its pyridinyl moiety both carrying the radioactive halogen (fluorine-18) and allowing its efficient incorporation via a nucleophilic heteroaromatic substitution, and its 2-bromoacetamide function, ensuring the efficient alkylation of a phosphorothioate monoester group born at the 3'- or 5'-end of single-stranded oligonucleotides. [(18)F]FPyBrA (HPLC-purified) was efficiently prepared in 18-20% non-decay-corrected yield (based on starting [(18)F]fluoride) using a three-step radiochemical pathway in 80-85 min. The developed procedure involves (1) a high-yield nucleophilic heteroaromatic ortho-radiofluorination as the fluorine-18 incorporation-step (70-85% radiochemical yield) and uses [3-(3-tert-butoxycarbonylaminopropoxy)pyridin-2-yl]trimethylammonium trifluoromethanesulfonate as precursor for labeling, followed by (2) rapid and quantitative TFA-removal of the N-Boc-protective group and (3) condensation with 2-bromoacetyl bromide (45-65% radiochemical yield). Typically, 3.3-3.7 GBq (90-100 mCi) of HPLC-purified [(18)F]FPyBrA could be obtained in 80-85 min, starting from 18.5 GBq (500 mCi) of a cyclotron production batch of [(18)F]fluoride. [(18)F]FPyBrA was regioselectively conjugated with 9-mer and 18-mer single-stranded oligonucleotides, provided with a phosphorothioate monoester group at their 3'-end. Both natural phosphodiester DNAs and in vivo-stable 2'-methoxy and -fluoro-modified RNAs were used. Conjugation uses optimized, short-time reaction conditions (MeOH/0.1 M PBS pH 7.4, 15 min, 120 degrees C), both compatible with the chemical stability of the oligonucleotides (ONs) and the half-life of fluorine-18. Conjugated [(18)F]ONs were finally purified by RP-HPLC and desalted using a Sephadex NAP-10 column. The whole radiosynthetic procedure, including the preparation of the fluorine-18-labeled reagent, the conjugation with the oligonucleotide, and the HPLC purification and formulation lasted 140-160 min. [(18)F]FPyBrA represents a valuable alternative to the already reported N-(4-[(18)F]fluorobenzyl)-2-bromoacetamide for the design and development of oligonucleotide-based radiopharmaceuticals for PET.
Photon-counting single-molecule spectroscopy for studying conformational dynamics and macromolecular interactions (2002)

Single-molecule methods have the potential to provide information about conformational dynamics and molecular interactions that cannot be obtained by other methods. Removal of ensemble averaging provides several benefits, including the ability to detect heterogeneous populations and the ability to observe asynchronous reactions. Single-molecule diffusion methodologies using fluorescence resonance energy transfer (FRET) are developed to monitor conformational dynamics while minimizing perturbations introduced by interactions between molecules and surfaces. These methods are used to perform studies of the folding of Chymotrypsin Inhibitor 2, a small, single-domain protein, and of single-stranded DNA (ssDNA) homopolymers. Confocal microscopy is used in combination with sensitive detectors to detect bursts of photons from fluorescently labeled biomolecules as they diffuse through the focal volume. These bursts are analyzed to extract fluorescence resonance energy transfer (FRET) efficiency. Advances in data acquisition and analysis techniques that are providing a more complete picture of the accessible molecular information are discussed. Photon Arrival-time Interval Distribution (PAID) analysis is a new method for monitoring macromolecular interactions by fluorescence detection with simultaneous determination of coincidence, brightness, diffusion time, and occupancy (proportional to concentration) of fluorescently-labeled molecules undergoing diffusion in a confocal detection volume. This method is based on recording the time of arrival of all detected photons, and then plotting the two-dimensional histogram of photon pairs, where one axis is the time interval between each pair of photons 1 and 2, and the second axis is the number of other photons detected in the time interval between photons 1 and 2. PAID is related to Fluorescence Correlation Spectroscopy (FCS) by a collapse of this histogram onto the time interval axis. PAID extends auto- and cross-correlation FCS by measuring the brightness of fluorescent species. A data-fitting model is developed, which is used to simultaneously determine coincidence, brightness, diffusion time, and occupancy from experiments performed on fluorophore-labeled dsDNA test samples. Using simulations, the performance of PAID is compared with existing methods. The statistical accuracy of the parameters extracted using PAID exceeds or matches the accuracy of the other methods, while providing additional information.
Stability of a Class of Direct Adaptive Control Systems with Input Amplitude Constraint (1995)

This paper presents stability and tracking results for discrete time direct adaptive control systems with input saturation constraints. It is shown that the adaptive closed loop system for a class of stable type-1 plants is stable in the sense that the plant output remains bounded. It is also shown that the adaptive control law converges to the corresponding non adaptive one with known plant parameters as time approaches infinity.
A Comparison of the Effect of pH on the Biodegradation of Two Synthetic Absorbable Sutures (1982)

The differences in hydrolytic degradation of two size 2-0 synthetic absorbable sutures, Polyglycolic acid (Dexon) and Poly(glycolide-lactide) (Vicryl), in the buffer media of three different pH levels ranging from 5.25 to 10.09, were compared in terms of the percentage retention of tensile strength. It was found that Vicryl sutures, in general, exhibited better retention of tensile strength than Dexon sutures within the studied pH range and extent of hydrolysis. When comparing Vicryl with Dexon sutures, the former was only slightly better than Dexon sutures, in the case of an acid environment. The former, however, retained better strength than the latter in a physiological pH (7.44) and under high-alkaline conditions (10.09). Among the three pH levels tested, Vicryl sutures retained the highest tensile strength in a physiological pH and showed a faster loss of tensile strength in both acidic and high-alkaline conditions. Dexon sutures, however, exhibited better retention of tensile strength in the acidic medium than in an alkaline medium. This pH dependent hydrolysis of Dexon and Vicryl sutures should command surgeons' attention in their selection of synthetic absorbable suture materials for particular needs.
Heterologous Expression of Mammalian P450 Enzymes (1994)

Background and Strategy Yeast Mammalian Cells Baculovirus Bacteria Conclusion
The epigenetic implication in coronavirus infection and therapy (2020)

Epigenetics is a relatively new field of science that studies the genetic and non-genetic aspects related to heritable phenotypic changes, frequently caused by environmental and metabolic factors. In the host, the epigenetic machinery can regulate gene expression through a series of reversible epigenetic modifications, such as histone methylation and acetylation, DNA/RNA methylation, chromatin remodeling, and non-coding RNAs. The coronavirus disease 19 (COVID-19) is a highly transmittable and pathogenic viral infection. The Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), which emerged in Wuhan, China, and spread worldwide, causes it. COVID-19 severity and consequences largely depend on patient age and health status. In this review, we will summarize and comparatively analyze how viruses regulate the host epigenome. Mainly, we will be focusing on highly pathogenic respiratory RNA virus infections such as coronaviruses. In this context, epigenetic alterations might play an essential role in the onset of coronavirus disease complications. Although many therapeutic approaches are under study, more research is urgently needed to identify effective vaccine or safer chemotherapeutic drugs, including epigenetic drugs, to cope with this viral outbreak and to develop pre- and post-exposure prophylaxis against COVID-19.
The Influence of Alloy Composition and Retained Austenite on Scuffing Resistance (1987)

The influence of alloy composition Cr and Ni and retained austenite rated from 4.2% to 30.8% on scuffing resistance of gear steels was investigated by laboratory experiments with disc machine. The RA has a significant influence on scuffing resistance under light load. Under heavy load the alloy composition has greater influence than RA. It is not necessary to control the RA content in heavily loaded gears. The mechanism of such a phenomenon was discussed.
Transcutaneous electrical nerve stimulation in rheumatoid arthritis. (1983)

The therapeutic effect of once weekly transcutaneous electrical nerve stimulation in patients with rheumatoid arthritis was compared with placebo in a randomised, double-blind, non-crossover study lasting three weeks. Thirty-two patients with classic or definite rheumatoid arthritis and wrist involvement were evaluated. Transcutaneous electrical nerve stimulation was better than the placebo in relieving pain at rest and while gripping. In addition, grip strength, measured as power and work done, immediately improved following transcutaneous electrical nerve stimulation but returned almost to initial values between assessments. No significant improvement was shown for the placebo group.
Improved Liquid Chromatography-MS/MS of Heparan Sulfate Oligosaccharides via Chip-Based Pulsed Makeup Flow (2011)

Microfluidic chip-based hydrophilic interaction chromatography (HILIC) is a useful separation system for liquid chromatography-mass spectrometry (LC-MS) in compositional profiling of heparan sulfate (HS) oligosaccharides; however, ions observed using HILIC LC-MS are low in charge. Tandem MS of HS oligosaccharide ions with low charge results in undesirable losses of SO(3) from precursor ions during collision induced dissociation. One solution is to add metal cations to stabilize sulfate groups. Another is to add a nonvolatile, polar compound such as sulfolane, a molecule known to supercharge proteins, to produce a similar effect for oligosaccharides. We demonstrate use of a novel pulsed makeup flow (MUF) HPLC-chip. The chip enables controlled application of additives during specified chromatographic time windows and thus minimizes the extent to which nonvolatile additives build up in the ion source. The pulsed MUF system was applied to LC-MS/MS of HS oligosaccharides. Metal cations and sulfolane were tested as additives. The most promising results were obtained for sulfolane, for which supercharging of the oligosaccharide ions increased their signal strengths relative to controls. Tandem MS of these supercharged precursor ions showed decreased abundances of product ions from sulfate losses yet more abundant product ions from backbone cleavages.
A xylazine infusion regimen to provide analgesia in sheep (2001)

The efficacy of continuous low-dose xylazine infusion following an initial loading dose in providing analgesia in sheep was examined using an algesimetry method based on a leg lifting response to an electrical stimulus. Sheep received a 5 mg intramuscular injection of xylazine followed by continuous infusion of intravenous xylazine (2mg/h) for 90 min. This treatment resulted in significant increases in the level of current required to elicit a leg lifting response (287% of baseline) and steady state analgesia was maintained from 10 min after the start of the infusion until the end of the experimental period. This protocol appears to be a simple and effective regimen for providing steady state analgesia in sheep.
Reduced rank regression via adaptive nuclear norm penalization (2012)

Adaptive nuclear-norm penalization is proposed for low-rank matrix approximation, by which we develop a new reduced-rank estimation method for the general high-dimensional multivariate regression problems. The adaptive nuclear norm of a matrix is defined as the weighted sum of the singular values of the matrix. For example, the pre-specified weights may be some negative power of the singular values of the data matrix (or its projection in regression setting). The adaptive nuclear norm is generally non-convex under the natural restriction that the weight decreases with the singular value. However, we show that the proposed non-convex penalized regression method has a global optimal solution obtained from an adaptively soft-thresholded singular value decomposition. This new reduced-rank estimator is computationally efficient, has continuous solution path and possesses better bias-variance property than its classical counterpart. The rank consistency and prediction/estimation performance bounds of the proposed estimator are established under high-dimensional asymptotic regime. Simulation studies and an application in genetics demonstrate that the proposed estimator has superior performance to several existing methods. The adaptive nuclear-norm penalization can also serve as a building block to study a broad class of singular value penalties.
HPV DNA Testing in Cervical Cancer Screening (2000)

In this study population, a cut point of 1.0 pg/mL using the second generation assay permitted sensitive detection of cervical high-grade lesions and cancer, yielding an apparently optimal trade-off between high sensitivity and reasonable specificity for this test. The test will perform best in settings in which sensitive detection of high-grade lesions and cancer is paramount. Because HPV prevalence varies by population, HPV testing positive predictive value for detection of high-grade lesions and cancer will vary accordingly, with implications for utility relative to other cervical cancer screening methods.
Clovamide and rosmarinic acid induce neuroprotective effects in<i>in vitro</i>models of neuronal death (2009)

Clovamide and rosmarinic acid are neuroprotective compounds of potential use at the nutritional/pharmaceutical interface.
Atmospheric Emissions Photometric Imaging Experiment For Spacelab 1 (1983)

The atmospheric emissions photometric imaging (AEPI) experiment to be flown on Spacelab 1 is designed to study faint natural and artificial atmospheric emission phenomena. Atmospheric emissions in the spectral region 2000 A to 7500 A from two optical channels, wide angle and telephoto, are detected by an image-enhanced low-light-level TV system. A third, telephoto, optical channel images onto the photocathode of a microchannel plate photomultiplier tube having 100 discrete anodes. Photons are counted for each discrete anode, providing a direct measure of the luminosity of an object viewed by the TV telephoto lens, albeit with low spatial resolution. Detector pointing in the range ±40°x±80°exclusive of restrictions due to the proximity of other experiments is provided by a two-axis gimbal made from a surplus Apollo telescope mount (MAST). The pointing stability is 1 arc min with respect to the spacecraft coordinate system for an exposure of one s, and the tracking capability is 3.5 deg - s-1 with a stability of 1 arc min. The detector and pointing system are located on the Spacelab pallet and are controlled by stored programs resident in the dedicated experiment processor located in the Spacelab module.
Fibroblast Growth Factor 23 (FGF23) and Disorders of Phosphate Metabolism (2009)

<p/> <p>Derangements in serum phosphate level result in rickets/osteomalacia or ectopic calcification indicating that healthy people without these abnormalities maintain serum phosphate within certain ranges. These results indicate that there must be a regulatory mechanism of serum phosphate level. Fibroblast growth factor 23 (FGF23) was identified as the last member of FGF family. FGF23 is produced by bone and reduces serum phosphate level by suppressing phosphate reabsorption in proximal tubules and intestinal phosphate absorption through lowering 1,25-dihydroxyvitamin D level. It has been shown that excess and deficient actions of FGF23 result in hypophosphatemic rickets/osteomalacia and hyperphosphatemic tumoral calcinosis, respectively. These results indicate that FGF23 works as a hormone, and several disorders of phosphate metabolism can be viewed as endocrine diseases. It may become possible to treat patients with abnormal phosphate metabolism by pharmacologically modifying the activity of FGF23.</p>
Suppressor Cells and Immunoregulation (1984)

We have described a model system of immunoregulation in which gene products associated with both the major histocompatibility complex and the heavy-chain immunoglobulin gene complex guide a series of cellular interactions. The Igh genetic restrictions may represent the use of internal images of antigen and idiotype as suppressor-T cell receptors. The data indicate that the T-cell and B-cell Igh products are distinct. The T cell-derived idiotype-like determinants are used for suppressor-T cell communications. The MHC restrictions generally involve the I-J subregion. These restrictions are imposed by the presentation of antigen or suppressor factor by specialized populations of I-J bearing accessory cells. The role of MHC products in the induction of suppressor cells has several homologies with the mechanisms responsible for the induction of H-2-restricted helper cells. First, I-region products on specialized presenting cells determine the specificity and genetic restrictions of the T cells. Thus, recognition of antigen in the context of I-A and I-E products is required for helper-T cell induction, and similarly the various suppressor-T cell subsets recognize antigen or suppressor factor presented in the context of I-J subregion-encoded antigens. Furthermore, the data suggest that the suppressor cells bear receptors for self I-J products. As an additional analogy between suppressor and helper cells, we have shown that in H-2-heterozygous F1 animals at least two populations of suppressor cells can be induced, one specific for each parental H-2 haplotype. The NP and ABA suppressor-cell pathways consist of multiple cellular elements, including at least three and possibly four distinct T-cell populations and two or more distinctive accessory cell populations. These are summarized in Figure 1. The specific soluble suppressor factors produced by each suppressor-T cell subset are involved in cellular communication processes. The terminal phases of the suppressor-cell cascade are antigen dependent but involve nonspecific bystander effects. In this review we have indicated the numerous homologies between the data in the hapten systems studied in our laboratories and the various other suppressor-cell models previously described in the literature.