gebhart/patrepeval huggingface dataset:
Generation of 36-femtosecond pulses from a ytterbium fiber laser (2003)

By optimizing the cavity dispersion map, 1.5-nJ pulses as short as 36 fs are obtained from a Yb-doped fiber laser. Residual higher-order dispersion currently limits the pulse duration, and it should be possible to generate pulses as short as 25-30 fs with Yb-doped fiber.
RNA‐Cleaving Deoxyribozyme Sensor for Nucleic Acid Analysis: The Limit of Detection (2010)

Along with biocompatibility, chemical stability, and simplicity of structural prediction and modification, deoxyribozyme-based molecular sensors have the potential of an improved detection limit due to their ability to catalytically amplify signal. This study contributes to the understanding of the factors responsible for the limit of detection (LOD) of RNA-cleaving deoxyribozyme sensors. A new sensor that detects specific DNA/RNA sequences was designed from deoxyribozyme OA-II [Chiuman, W.; Li, Y. (2006) J. Mol. Biol. 357, 748-754]. The sensor architecture allows for a unique combination of high selectivity, low LOD and the convenience of fluorescent signal monitoring in homogeneous solution. The LOD of the sensor was found to be approximately 1.6 x 10(-10) M after 3 h of incubation. An equation that allows estimation of the lowest theoretical LOD using characteristics of parent deoxyribozymes and their fluorogenic substrates was derived and experimentally verified. According to the equation, "catalytically perfect" enzymes can serve as scaffolds for the design of sensors with the LOD not lower than approximately 2 x 10(-15) M after 3 h of incubation. A new value termed the detection efficiency (DE) is suggested as a time-independent characteristic of a sensor's sensitivity. The expressions for the theoretical LOD and DE can be used to evaluate nucleic acid and protein enzymes for their application as biosensing platforms.
Far-Red and Near-Infrared Seminaphthofluorophores for Targeted Pancreatic Cancer Imaging (2017)

Molecular probes that selectively highlight pancreatic cancer (PC) tissue have the potential to improve pancreatic ductal adenocarcinoma (PDAC) margin assessment through the selective highlighting of individual PC cells. Herein, we report a simple and unique family of systematically modified red and near-infrared fluorescent probes that exhibit a field-effect-derived redshift. Two of thirteen probes distributed to the normal mouse pancreas following systemic administration. One selectively accumulated in genetically modified mouse models of PDAC. The probe exhibited intracellular accumulation and enabled visualization of four levels of the structure, including the whole organ, resected tissue, individual cells, and subcellular organelles. In contrast to the small-molecule probes reported previously, it possesses an inherent affinity toward PDAC cells and thus does not require conjugation to any targeting agent. The fluorescent probe can thus promote new strategies not only for precision image-guided surgery, but also for PC detection, monitoring of therapeutic outcomes, and basic research.
RoomNet: End-to-End Room Layout Estimation (2017)

This paper focuses on the task of room layout estimation from a monocular RGB image. Prior works break the problem into two sub-tasks: semantic segmentation of floor, walls, ceiling to produce layout hypotheses, followed by an iterative optimization step to rank these hypotheses. In contrast, we adopt a more direct formulation of this problem as one of estimating an ordered set of room layout keypoints. The room layout and the corresponding segmentation is completely specified given the locations of these ordered keypoints. We predict the locations of the room layout keypoints using RoomNet, an end-to-end trainable encoder-decoder network. On the challenging benchmark datasets Hedau and LSUN, we achieve state-of-the-art performance along with 200x to 600x speedup compared to the most recent work. Additionally, we present optional extensions to the RoomNet architecture such as including recurrent computations and memory units to refine the keypoint locations under the same parametric capacity.
Very high efficiency InGaP/GaAs dual-junction solar cell manufacturing at Emcore Photovoltaics (2002)

The electrical performance and space qualification data of very high efficiency dual junction n/p InGaP/GaAs (on Ge) solar cells manufactured at Emcore Photovoltaics are described. The minimum average beginning-of-life (BOL) conversion efficiency of large area (27.5 cm/sup 2/) solar cells currently in production is 23.0% (28/spl deg/C, 1 sum AM0, 135.3 mW/cm/sup 2/). The resulting power output per cell is 0.86 watts. The highest efficiency obtained of 25.3% represents a record for a large area dual junction cell. The results presented here are for solar cells that have an optimized end-of-life (EOL) structure. The power remaining factors after irradiation with 1-MeV electrons at fluences of 5E14, 1E15, and 3E15 e/cm/sup 2/ are 0.89, 0.84, and 0.74 respectively. The results of full space qualification testing including electrical, radiation exposure, temperature coefficients, thermal cycling, humidity, optical, and mechanical measurements are presented.
Different designs and limits of longitudinal magnetic recording media (2002)

Combining antiferromagnetically coupled (AFC) and laminated media (LM), we were able to incorporate the stability of AFC media with the signal-to-noise ratio (SNR) of LM. This improvement is directly related to the decrease of the demagnetization field which is calculated to be up to 50% of the LM case for some configurations of AFC+LM that were experimentally realized. The stabilization layer also increases the magnetic energy barrier of the recording layers in the AFC+LM structure, which further improves the stability of this media. An improvement of ∼1 dB in SNR was observed in AFC+LM in comparison to single layer media.
Pimavanserin - a potential first line therapy for Parkinson's disease psychosis (2009)

Pimavanserin (ACP-103) is a novel and selective 5-HT 2A inverse agonist currently in Phase III development for Parkinsonâ€™s disease psychosis (PDP). Â  Most antipsychotic drugs cannot be used for this indication because their prominent dopamine receptor blocking potency counteracts the dopamine replacement therapy used to alleviate the motor symptoms of Parkinsonâ€™s disease (PD). Â  There is currently no first line therapy approved for PDP in the EU or the USA despite the well established unmet medical need for this indication which afflicts up to 40% of PD patients. Â  In addition to demonstrating activity in a variety of established animal models of schizophrenia (JPET, 317 , 910-918, 2006 ), we have now shown that pimavanserin, in contrast to currently marketed antipsychotics, potentially normalizes deficits in prepulse inhibition and augmented behavioral responses to amphetamine in rodent models of PDP while not counteracting L-DOPA induced turning in unilaterally lesioned rodents. Â  These data, which indicate that pimavanserin can be differentiated from current antipsychotic off-label therapy for PDP by providing antipsychotic activity, while allowing for optimal motor control, are further supported by a proof-of-concept Phase II study which demonstrated that doses of pimavanserin that do not worsen motor symptoms of PD provide a clinically meaningful reduction in psychosis in patients with PDP as measured by the SAPS. Â  ACADIA Pharmaceuticals Inc., San Diego, USA
Analytical Separations Using Molecular Micelles in Open-Tubular Capillary Electrochromatography (2002)

Open-tubular capillary electrochromatography (OT-CEC) is an alternative approach to conventional CEC. The primary advantage of OT-CEC is the elimination of problems associated with frits and silica particles in conventional CEC. This report is an investigation of the utility of using a polymeric surfactant (molecular micelle) for OT-CEC. In this approach, fused-silica capillaries coated with thin films of physically adsorbed charged polymers are developed by use of a polyelectrolyte multilayer (PEM) coating procedure. The PEM coating is constructed in situ by alternating rinses with positively and negatively charged polymers, where the negatively charged polymer is a molecular micelle. This can offer a number of advantages for separation of hydrophobic analytes. In this study, poly(diallyldimethylammonium chloride) was used as the cationic polymer and poly(sodium N-undecanoyl-L-glycinate) was used as the anionic polymer for PEM coating. The performance of the modified capillaries as a separation medium is evaluated by use of seven benzodiazepines as analytes. The run-to-run, day-to-day, week-to-week, and capillary-to-capillary reproducibilities of electroosmotic flow are very good with relative standard deviation values of less than 1% in all cases. In addition, the chromatographic performance of the monomeric form of the molecular micelle is compared for the separation of these analytes. The PEM-coated capillary was remarkably robust with more than 200 runs accomplished in this study. Strong stability against extreme pH values was also observed. The general utility of this approach is discussed in detail.
7-Substituted 5-Amino-2-(2-furyl)pyrazolo[4,3-<i>e</i>]-1,2,4-triazolo[1,5-<i>c</i>]pyrimidines as A<sub>2A</sub>Adenosine Receptor Antagonists:  A Study on the Importance of Modifications at the Side Chain on the Activity and Solubility (2001)

It was demonstrated in the early 1990s that adenosine exerts many physiological functions through the interaction with four different receptors, named A1, A2A, A2B, and A3. In the past few years, our group has been involved in the development of A2A antagonists, which led to the synthesis of SCH 58261 (1), the first potent and selective adenosine A2A antagonist, which has been widely used as a reference compound. In this paper, we present an extended series of pyrazolotriazolopyrimidines synthesized with the aim to investigate the influence of the substitutions on the pyrazole ring. The choice of the substituents was based on their capability to improve water solubility while retaining high affinity and selectivity at the human A2A adenosine receptor subtype. In this series, some structural characteristics that are important for activity, i.e., tricyclic structure, free amino group at 5-position, furan ring, and substituent at 7-position on the pyrazole moiety, have been maintained. We focused our attention on the nature of the phenyl ring substituent to improve water solubility. Following this strategy, we developed new compounds with good affinity and selectivity for A2A adenosine receptors, such as 8d (K(i) 0.12 nM; hA1/hA2A ratio = 1025; R(m) = 2.8), 8h (K(i) 0.22; hA1/hA2A ratio = 9818; R(m) = 3.4), 8i (K(i) 0.18 nM; hA1/hA2A ratio = 994; R(m) = 2.8), 8k (K(i) 0.13 nM; hA1/hA2A ratio = 4430; R(m) = 3.6), and 14b (K(i) 0.19 nM; hA1/hA2A ratio = 2273; R(m) = 2.7). All the new synthesized compounds have no significant interaction with either A2B or A3 receptor subtypes. This new series of compounds deeply enlightens some structural requirements to display high affinity and selectivity for the A2A adenosine receptor subtype, although our goal of identifying new compounds with increased water solubility was not completely achieved. On this basis, other strategies will be devised to improve this class of compounds with a profile that appears to be promising for treatment of neurodegenerative disorders, such as Parkinson's disease.
Photoactivatable DCDHF fluorophores for single-molecule imaging (2009)

We have designed and studied the photophysics of a class of organic fluorophores termed "DCDHFs," which were originally used as push-pull chromophores for nonlinear optical applications. In this paper, we describe the general photophysics of many realizations of the DCDHF class of single-molecule emitters. Moreover, we have reengineered a red-emitting DCDHF fluorophore so that it is dark until photoactivated with a short burst of low-intensity violet light. Photoactivation of the dark fluorogen leads to conversion of an azide to an amine, which shifts the absorption to long wavelengths. After photoactivation, the fluorophore is bright and photostable enough to be imaged on the singlemolecule level in living cells. This molecule and its relatives will provide a new class of bright photoactivatable fluorophores, as are needed for super-resolution imaging schemes that require active control of single-molecule emission.
Can We Disrupt the Sensing of Honey Bees by the Bee Parasite Varroa destructor? (2014)

These data indicate the potential of the selected compounds to disrupt the Varroa--honey bee associations, thus opening new avenues for Varroa control.
Kinetic study of the halogenation of imidazolidin-4-one derivatives (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTKinetic study of the halogenation of imidazolidin-4-one derivativesIsmat Naquib, Te Chen Tsao, Partha K. Sarathy, and S. Davis WorleyCite this: Ind. Eng. Chem. Res. 1992, 31, 8, 2046–2050Publication Date (Print):August 1, 1992Publication History Published online1 May 2002Published inissue 1 August 1992https://pubs.acs.org/doi/10.1021/ie00008a030https://doi.org/10.1021/ie00008a030research-articleACS PublicationsRequest reuse permissionsArticle Views105Altmetric-Citations6LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Isolation of the galactose-binding lectin that mediates the in vitro adherence of Entamoeba histolytica. (1987)

Entamoeba histolytica adheres to human colonic mucus, colonic epithelial cells, and other target cells via a galactose (Gal) or N-acetyl-D-galactosamine (GalNAc) inhibitable surface lectin. Blockade of this adherence lectin with Gal or GalNAc in vitro prevents amebic killing of target cells. We have identified and purified the adherence lectin by two methods: affinity columns derivatized with galactose monomers or galactose terminal glycoproteins, and affinity columns and immunoblots prepared with monoclonal antibodies that inhibit amebic adherence. By both methods the adherence lectin was identified as a 170-kD secreted and membrane-bound amebic protein. The surface location of the lectin was confirmed by indirect immunofluorescence. Purified lectin competitively inhibited amebic adherence to target cells by binding to receptors on the target Chinese hamster ovary cells in a Gal-inhibitable manner.
On-Chip Mirrorless-Oscillation in Nonlinear Silicon Waveguides using Non-degenerate Four-Wave Mixing (2011)

Nondegenerate mirrorless four-wave mixing oscillation with frequency tunability is proposed in silicon nonlinear waveguide by using higher spatial mode. As low as ~2W threshold power is obtained in several centimeters long waveguide.
High capacity CO2 adsorption in a Mg(ii)-based phosphine oxide coordination material (2011)

The new porous phosphine coordination material, PCM-11, is an unusual 8,4-connected coordination polymer with an open 3-D pore structure, formed by reaction of Mg(II) with tris(para-carboxylato)triphenylphosphine oxide. The highly ionic nature of the metal-ligand bonding results in excellent thermal stability upon desolvation (>460 °C). PCM-11 is easily activated for small molecule sorption at low temperature without the requirement for solvent pre-exchange. It adsorbs 47.5 wt% CO(2) at 11.6 bar and 30 °C.
PCR Cloning and Sequencing of the β-Amylase cDNA from Barley (1994)

Polymerase chain reaction (PCR) amplification of mRNA from developing barley (cultivar Haruna two-rows) endosperm was used to clone and sequence full-length cDNA encoding beta-amylase. The beta-amylase cDNA was 1,775 bp in length. The beta-amylase was deduced to be composed of 535 amino acid residues and its molecular weight was calculated to be 59,610. Kreis et al. reported that the beta-amylase cDNA from barley (cultivar Hiproly) was 1,754 bp in length and coded for a polypeptide of 535 amino acids [Eur. J. Biochem. (1987) 169, 517-525]. A comparison of the beta-amylase sequences from Haruna two-rows and Hiproly barleys revealed nine differences in the nucleotide sequence which resulted in three changes in the amino acid residues and 21 additional nucleotides at its 3'-end in the cultivar Haruna two-rows. The three changes were as follows: Ala-233, Ser-347, Met-527 (Haruna two-rows) and Val-233, Met-347, Ile-527 (Hiproly). Lundgard and Svensson pointed out that 23 amino acid residues of the peptide fragment derived from the COOH-terminal region of barley (cultivar Gula) beta-amylase were in agreement with the deduced amino acid sequence reported by Kreis et al., with the exception of a single position (Met-527 compared to Ile) [Carlsberg Res. Commun. (1986) 51, 487-491]. Our findings described above showed Met-527 is reasonable. In the cases of beta-amylases from soybean and sweet potato, the positions that corresponded to those at 233 and 347 in the amino acid sequence of beta-amylase from barley were Ala and Ser, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)
Deletion of E184L, a Putative DIVA Target from the Pandemic Strain of African Swine Fever Virus, Produces a Reduction in Virulence and Protection against Virulent Challenge (2021)

No commercial vaccines are available to prevent African swine fever (ASF). The ASF pandemic caused by the ASF virus Georgia 2010 (ASFV-G) strain is seriously affecting pork production in a contiguous geographical area from Central Europe to East Asia.
A 1-V 10b 40MS/s Pipelined ADC with Low-Voltage Circuit Techniques in 0.18 um CMOS (2009)

This paper presents a 1-V 10b 40MS/s Pipelined ADC with an effective resolution bandwidth up to 66MHz. Two low-voltage circuit techniques are employed: 1) feedback current biasing - which ensures the accuracy of the current source biasing over the process variations without the use of cascode structure in low-voltage environment; 2) low-voltage current-mode sub-ADCs - which allows current-mirror sharing between the low-voltage comparators thus implying a reduction in static power consumption. The proposed ADC was implemented in 0.18um IP6M CMOS technology. Chip measurement results show that the DNL and INL are within 0.9 and 1.1 LSB, respectively. The ADC maintains the SNDR over 54.5dB and 51.5dB for input frequencies of 9.1MHz and 66MHz, respectively. The active die area is 2.2mm and the ADC consumes 40mW at 1-V supply.
Production of mouse monoclonal antibodies which inhibit in vitro adherence of Entamoeba histolytica trophozoites (1986)

Adherence by axenic Entamoeba histolytica trophozoites to mammalian cells is mediated by an N-acetylgalactosamine (GalNAc)-inhibitable adhesin on the surface of the parasite. We isolated 35 hybridoma cell lines producing antibodies to E. histolytica as indicated by ELISA with sonicated amebic protein or by immunofluorescence assay with fixed whole trophozoites. Tissue culture supernatants were further screened for subcloning by the ability to bind to Chinese hamster ovary (CHO) cells which were first exposed to a partially purified soluble preparation of the amebic GalNAc-inhibitable lectin. Eight tissue culture supernatants were positive in this assay. Antibodies from four subcloned cell lines (D3-14, H8-5, I12-2, and I1-21) inhibited amebic adherence to CHO cells (P less than 0.01). Of the original 35 tissue culture supernatants, 3 also inhibited amebic adherence (P less than 0.01; F1, F14, and J10); monoclonal antibodies in these supernatants did not bind to lectin-exposed CHO cells. Three purified monoclonal antibodies (H8-5, I12-2, and I1-21) inhibited amebic adherence at greater than or equal to 2 micrograms/10(4) amebae (P less than 0.05). None of these inhibitory monoclonal antibodies immunoprecipitated with a soluble amebic protein preparation following sodium dodecyl sulfate-polyacrylamide gel electrophoresis under denaturing conditions. Monoclonal antibodies which inhibit in vitro adherence by E. histolytica will be useful in purification of the GalNAc-inhibitable lectin.
Making fast buffer insertion even faster via approximation techniques (2005)

As technology scales to 0.13 micron and below, designs are requiring buffers to be inserted on interconnects of even moderate length for both critical paths and fixing electrical violations. Consequently, buffer insertion is needed on tens of thousands of nets during physical synthesis optimization. Even the fast implementation of van Ginneken's algorithm requires several hours to perform this task. This work seeks to speed up the van Ginneken style algorithms by an order of magnitude while achieving similar results. To this end, we present three approximation techniques in order to speed up the algorithm: (1) aggressive pre-buffer slack pruning, (2) squeeze pruning, and (3) library lookup. Experimental results from industrial designs show that using these techniques together yields solutions in 9 to 25 times faster than van Ginneken style algorithms, while only sacrificing less than 3% delay penalty.
Hemocompatibility of Superhemophobic Titania Surfaces (2016)

The hemocompatibility of superhemophobic surfaces is investigated and compared with that of hemophobic surfaces and hemophilic surfaces. This analysis indicates that only those superhemophobic surfaces with a robust Cassie-Baxter state display significantly lower platelet adhesion and activation. It is envisioned that the understanding gained through this work will lead to the fabrication of improved hemocompatible, superhemophobic medical implants.
Click beetle luciferase mutant and near infrared naphthyl-luciferins for improved bioluminescence imaging (2018)

The sensitivity of bioluminescence imaging in animals is primarily dependent on the amount of photons emitted by the luciferase enzyme at wavelengths greater than 620 nm where tissue penetration is high. This area of work has been dominated by firefly luciferase and its substrate, D-luciferin, due to the system's peak emission (~ 600 nm), high signal to noise ratio, and generally favorable biodistribution of D-luciferin in mice. Here we report on the development of a codon optimized mutant of click beetle red luciferase that produces substantially more light output than firefly luciferase when the two enzymes are compared in transplanted cells within the skin of black fur mice or in deep brain. The mutant enzyme utilizes two new naphthyl-luciferin substrates to produce near infrared emission (730 nm and 743 nm). The stable luminescence signal and near infrared emission enable unprecedented sensitivity and accuracy for performing deep tissue multispectral tomography in mice.