gebhart/patrepeval huggingface dataset:
Adiponectin Deficiency Increases Allergic Airway Inflammation and Pulmonary Vascular Remodeling (2009)

Obesity is associated with an increased incidence and severity of asthma, as well as other lung disorders, such as pulmonary hypertension. Adiponectin (APN), an antiinflammatory adipocytokine, circulates at lower levels in the obese, which is thought to contribute to obesity-related inflammatory diseases. We sought to determine the effects of APN deficiency in a murine model of chronic asthma. Allergic airway inflammation was induced in APN-deficient mice (APN(-/-)) using sensitization without adjuvant followed by airway challenge with ovalbumin. The mice were then analyzed for changes in inflammation and lung remodeling. APN(-/-) mice in this model develop increased allergic airway inflammation compared with wild-type mice, with greater accumulation of eosinophils and monocytes in the airways associated with elevated lung chemokine levels. Surprisingly, APN(-/-) mice developed severe pulmonary arterial muscularization and pulmonary arterial hypertension in this model, whereas wild-type mice had only mild vascular remodeling and comparatively less pulmonary arterial hypertension. Our findings demonstrate that APN modulates allergic inflammation and pulmonary vascular remodeling in a model of chronic asthma. These data provide a possible mechanism for the association between obesity and asthma, and suggest a potential novel link between obesity, inflammatory lung disease, and pulmonary hypertension.
<i>C</i><sub>3</sub>-Symmetrical Supramolecular Architectures:  Fibers and Organic Gels from Discotic Trisamides and Trisureas (2002)

Hydrogen bonded C(3)-symmetrical molecules that associate into supramolecular stacks are described. Structural mutation on these molecules has been performed to elucidate the contribution of the different secondary interactions (hydrogen bonding, pi-pi stacking) to the self-assembly of the disks into chiral stacks. Twelve C(3)-symmetrical molecules have been investigated, six of which contain three central amide functionalities (1a-f) and six of which contain three central urea groups (2a-f). Peripheral groups of the disks are "small", "medium", or "large", half of them being achiral and the other half being chiral, to enable investigation of the supramolecular architectures with CD spectroscopy. In all of the cases, elongated, helical stacks are formed in apolar solution, except for the "medium" amide disks 1c/d. The elongated stacks of the C(3)-symmetrical disks form gels, which are visualized by AFM and SANS, and this confirms the directionality of the interactions. For the "large" urea disk, 2f, fibers with a length of up to 2 microm are observed. Temperature dependent and "sergeants-and-soldiers" CD measurements reveal that the urea stacks are much more rigid than the corresponding amide ones. In case of the "medium" urea disks, 2c/d, a true rigid rod, is formed. Where amide disks immediately reach their thermodynamic equilibrium, kinetic factors seem to govern urea aggregation. In a number of experiments aimed at reversibility with the urea stacks, hysteresis is observed, implying that these urea disks initially form a poorly defined stack, which subsequently transforms slowly into a well-defined, chiral architecture.
Noninvasive liver diseases detection based on serum surface enhanced Raman spectroscopy and statistical analysis (2015)

In this paper, we investigated the feasibility of using surface enhanced Raman spectroscopy (SERS) of blood serum to discriminate liver cancer and liver cirrhosis patients from normal people. Serum taken from 44 healthy people, 45 liver cancer patients, 42 post-treatment liver cancer patients and 45 liver cirrhosis patients was measured. SERS peaks from these groups were compared and the assignments and biomedical meanings were analyzed and explained. In addition, support vector machine (SVM), partial least square-discriminant analysis (PLS-DA) and artificial neural networks (ANN) was used on the obtained SERS spectra to identify its diagnostic potential for liver diseases. PLS-SVM, PLS-DA and PLS-ANN indicated 91.5%, 89.2% and 90.3% accuracy, respectively. This preliminary study demonstrates that serum SERS can be used for liver cancer screening.
Identification of targetable kinases in idiopathic pulmonary fibrosis (2022)

We performed a comprehensive kinase expression analysis to explore the potential therapeutic targets for IPF. We found that DCLK1 and STK33 may serve as potential candidate targets for molecular targeted therapy of IPF. In addition, PDK4, ERBB4, PIM2, and SYK might also serve as personalized therapeutic targets of IPF. Additional large-scale studies are warranted to develop personalized therapies for patients with IPF.
On Quadratic Inverses for Quadratic Permutation Polynomials over Integer Rings (2005)

An interleaver is a critical component for the channel coding performance of turbo codes. Algebraic constructions are of particular interest because they admit analytical designs and simple, practical hardware implementation. Sun and Takeshita have recently shown that the class of quadratic permutation polynomials over integer rings provides excellent performance for turbo codes. In this correspondence, a necessary and sufficient condition is proven for the existence of a quadratic inverse polynomial for a quadratic permutation polynomial over an integer ring. Further, a simple construction is given for the quadratic inverse. All but one of the quadratic interleavers proposed earlier by Sun and Takeshita are found to admit a quadratic inverse, although none were explicitly designed to do so. An explanation is argued for the observation that restriction to a quadratic inverse polynomial does not narrow the pool of good quadratic interleavers for turbo codes.
Ahnak functions as a tumor suppressor via modulation of TGFβ/Smad signaling pathway (2014)

We provide detailed mechanisms of Ahnak-mediated potentiation of transforming growth factor β (TGFβ) signaling, which leads to a negative regulation of cell growth. We show that Smad3 interacts with Ahnak through MH2 domain and that Ahnak stimulates Smad3 localization into nucleus leading to potentiating TGFβ-induced transcriptional activity of R-Smad. Moreover, overexpression of Ahnak resulted in growth retardation and cell cycle arrest through downregulation of c-Myc and cyclin D1/D2. We describe results from analyses of Ahnak(-/-) mouse model expressing middle T antigen in a mammary gland-specific manner (MMTV(Tg/+)Ahnak(-/-)), which showed significantly progressed hyperplasia of mammary glands compared with MMTV(Tg/+)Ahnak(+/+). Finally, we screened multiple human breast cancer tissues and showed that the expression of Ahnak in cancer tissues is lower than that in control tissues by 50%. Taken together, these data indicate that Ahnak mediates a negative regulation of cell growth and acts as novel tumor suppressor through potentiation of TGFβ signaling.
Precise orientation of SPOT panchromatic images with tie points to a SAR image (2002)

© 2002 International Society for Photogrammetry and Remote Sensing. All rights reserved. The extraction of spatial data from satellite imagery requires that precise sensor models are used to orientate images. This is particularly important with images acquired by linear sensors, such as SPOT, frequently pointed with large incidence angles. Image orientation with pixel accuracy requires that accurate ground control points are used. The acquisition of ground control is especially difficult in remote areas, where satellite images are important data sources for the production and updating of topographic maps. This paper describes a method for the orientation of SPOT panchromatic images making use of tie points with a SAR image. SAR and SPOT images compose stereo-pairs with a good stereo intersection, from which heights can be derived. For the SAR-SPOT tie-points, approximate heights are derived from parallaxes and can be corrected using altimetric ground control points. The method described makes use of the fact that the SAR image orientation can be derived from the orbit and SAR processing parameters, provided with SAR images, and does not require ground control points. The paper reports on a study carried out with SPOT and Radarsat images from Portugal. Accuracy assessments were done with digital cartography and field surveyed GPS data. It was possible to conclude that using a SAR image, accurate SPOT image orientation can be achieved requiring only very few altimetric control points.
The catalytic subunit of pseutarin C, a group C prothrombin activator from the venom of Pseudonaja textilis, is structurally similar to mammalian blood coagulation factor Xa (2004)

Pseutarin C, a group C prothrombin activator from Pseudonaja textilis venom, is a large protein complex consisting of catalytic and nonenzymatic subunits, which are functionally similar to the mammalian FXa-FVa complex. Here, we present the complete cDNA sequence of the catalytic subunit of pseutarin C. The cDNA of the catalytic subunit encodes a protein of 449 amino acids, which includes a 22-residue signal peptide, 18-residue propeptide and a mature protein of 409 amino acids. The deduced amino acid sequence shows 74-83% identity to group D prothrombin activators from snake venom and approximately 42% identity to mammalian FX and has identical domain structure. The precursor of the catalytic subunit of pseutarin C has several unique features. The activation peptide of the catalytic subunit of pseutarin C is significantly smaller (27 as compared to 52 residues in mammalian FX) and does not contain any glycosylation sites. Unlike coagulation FXa, Ser52 and Asn45 of the light and heavy chains are O- and N-glycosylated in pseutarin C catalytic sub-unit. There is a 12-residue insertion in pseutarin C catalytic sub-unit close to the region that is implicated in binding to FVa. This is the first sequence of the catalytic subunit of a group C prothrombin activator.
Microvias effect on high‐frequency signal integrity (2002)

Microvias or high density interconnects (HDI) printed circuits are now being designed in ever increasing quantities. HDI brings some interesting new solutions to age‐old signal integrity (SI) concerns, and concerns that will grow as rise‐times continue to drop.This article focuses on five major areas of SI concerns—(1) noise: (a) noise‐reflections, (b) noise‐crosstalk, (c) noise‐simultaneous switching; (2) electro‐magnetic interference (EMI); (3) interconnect delays.In each case, HDI offers improvements and alternatives—but it is not a panacea. A couple of “cautions” are listed that can be a major stumbling block to HDI implementation, fortunately, they are not SI based. Important to SI is the materials used in HDI. Although not the focus of this article, the materials selected, as well as the dimensional stack‐up and PCB design rules, will influence SI and electrical performance (impedance, crosstalk and signal conditioning). Miniaturization provided by HDI will be a major contributor to SI performance.Finally, the SI example is also a case study in cost reduction. The “before” and “after” conditions are reviewed to emphasize the cost reduction and “time‐to‐market” advantages of HDI technology.
Application of Artificial Intelligence to Command and Control Systems (1988)

From the Publisher:
Topics covered include a categorization and review of intelligent multi-sensor data fusion, as well as its application to the naval and land environments. Techniques adopted include blackboard and object oriented knowledge based systems
intelligent situation assessment, and intelligent command and
Neutralization of Transthyretin Reverses the Neuroprotective Effects of Secreted Amyloid Precursor Protein (APP) in APP<sub>Sw</sub>Mice Resulting in Tau Phosphorylation and Loss of Hippocampal Neurons: Support for the Amyloid Hypothesis (2004)

Alzheimer's disease (AD) may be caused by the abnormal processing of the amyloid precursor protein (APP) and the accumulation of beta-amyloid (Abeta). The amyloid precursor protein can be proteolytically cleaved into multiple fragments, many of which have distinct biological actions. Although a high level of Abeta can be toxic, the alpha-secretase cleaved APP (sAPPalpha) is neuroprotective. However, the mechanism of sAPPalpha protection is unknown. Here, we show that sAPPalpha increases the expression levels of several neuroprotective genes and protects organotypic hippocampal cultures from Abeta-induced tau phosphorylation and neuronal death. Antibody interference and small interfering RNA knock-down demonstrate that the sAPPalpha-driven expression of transthyretin and insulin-like growth factor 2 is necessary for protection against Abeta-induced neuronal death. Mice overexpressing mutant APP possess high levels of sAPPalpha and transthyretin and do not develop the tau phosphorylation or neuronal loss characteristic of human AD. Chronic infusion of an antibody against transthyretin into the hippocampus of mice overexpressing APP with the Swedish mutation (APP(Sw)) leads to increased Abeta, tau phosphorylation, and neuronal loss and apoptosis within the CA1 neuronal field. Therefore, the elevated expression of transthyretin is mediated by sAPPalpha and protects APP(Sw) mice from developing many of the neuropathologies observed in AD.
Identification and characterization of the <i>Pasteurella multocida</i> toxin translocation domain (2004)

Summary The Pasteurella multocida toxin (PMT) is a potent mitogen which enters the cytosol of eukaryotic cells via a low pH membrane translocation event. In common with the Escherichia coli cytotoxic necrotizing factor 1 (CNF1), the core of the PMT translocation domain is composed of two predicted hydrophobic helices (H1 – residues 402–423, H2 – 437–457) linked by a hydrophilic loop (PMT‐TL – 424–436). The peptide loop contains three acidic residues (D425, D431 and E434), which may play a role equivalent to D373, D379 and E382/383 in CNF1. To test this hypothesis, a series of point mutants was generated in which acidic residues were mutated into the permanently charged positive residue lysine. Individual mutation of D425, D431 and E434 each caused a four‐ to sixfold reduction in toxin activity. Interestingly, mutation of D401 located immediately outside the predicted helix–loop–helix motif completely abolished toxin activity. Individual mutations did not affect cell binding nor greatly altered toxin structure, but did prevent translocation of the surface‐bound proteins into the cytosol after a low pH pulse. Moreover, we demonstrate using an in vitro assay that PMT undergoes a pH‐dependent membrane insertion.
An Agent-Based Architecture for Handover Initiation and Decision in 4G Networks (2005)

The rollout of 3G networks coincides with the emergence of a variety of wireless access technologies (IEEE 802.11x, Bluetooth, etc.) supporting high bitrates in restricted areas and being complementary to wide coverage mobile networks. Integration of the different wireless access technologies is the general trend towards 4G. Our work focuses on the area of handover management in the context of 4G, and specifically on the initiation and decision phases of the handover mechanism. In 3G or earlier generation networks, handovers are initiated and decided by the base station on the basis of measurements of perceived SNR, cell congestion, terminal speed, etc. In 4G, due to the diversity of available radio access services, additional factors also need to be taken into account. We propose an agent-based architecture that determines the timing and target network of handovers with respect to user, application and terminal constraints.
Capture and Recycling of Sortase A through Site‐Specific Labeling with Lithocholic Acid (2016)

Enzyme-mediated protein modification often requires large amounts of biocatalyst, adding significant costs to the process and limiting industrial applications. Herein, we demonstrate a scalable and straightforward strategy for the efficient capture and recycling of enzymes using a small-molecule affinity tag. A proline variant of an evolved sortase A (SrtA 7M) was N-terminally labeled with lithocholic acid (LA)-an inexpensive bile acid that exhibits strong binding to β-cyclodextrin (βCD). Capture and recycling of the LA-Pro-SrtA 7M conjugate was achieved using βCD-modified sepharose resin. The LA-Pro-SrtA 7M conjugate retained full enzymatic activity, even after multiple rounds of recycling.
Localization of α1,3-fucosyltransferase VI in Weibel–Palade bodies of human endothelial cells (2000)

Surface glycosylation of endothelial cells is relevant to various processes including coagulation, inflammation, metastasis, and lymphocyte homing. One of the essential sugars involved in these processes is fucose linked alpha1-->3 to N-acetylglucosamine. A family of alpha1,3-fucosyltransferases (FucTs) called FucT-III, IV, V, VI, VII, and IX is able to catalyze such fucosylations. Reverse transcription-PCR analysis revealed that human umbilical vein endothelial cells express all of the FucTs except FucT-IX. The predominant activity, as inferred by acceptor specificity of enzyme activity in cell lysates, is compatible with the presence of FucT-VI. By using an antibody to recombinant soluble FucT-VI, the enzyme colocalized with beta4-galactosyltransferase-1 to the Golgi apparatus. By using a polyclonal antiserum raised against a 17-aa peptide of the variable (stem) region of the FucT-VI, immunocytochemical staining of FucT-VI was restricted to Weibel-Palade bodies, as determined by colocalization with P-selectin and von Willebrand factor. SDS/PAGE immunoblotting and amino acid sequencing of internal peptides confirmed the identity of the antigen isolated by the peptide-specific antibody as FucT-VI. Storage of a fucosyltransferase in Weibel-Palade bodies suggests a function independent of Golgi-associated glycosylation.
Non-sequence-specific antimalarial activity of oligodeoxynucleotides (1994)

The effects of exogenously applied oligodeoxynucleotides on Plasmodium falciparum proliferation was investigated. A fluorescence-activated cell sorter assay was employed to measure parasitemia after administration of either phosphodiester or phosphorothioate oligodeoxynucleotides. We report sequence-independent antimalarial activity preferentially with phosphorothioate congeners with IC50 values in the 1-2 microM range. Phosphorothioate oligodeoxynucleotides which were antisense, sense or nonsense to Plasmodium mRNA, as well as homopolymers (30-mers containing all A or T bases) were equally effective inhibitors of parasitemia. The antimalarial activity was dependent upon oligomer length, concentration, and time of addition to the cultures but was independent of the parasite strain tested. Four P. falciparum strains, including a multi-drug-resistant strain (MDR-K), a drug-sensitive strain (FCR-3), a erythrocyte membrane sialic acid-independent strain (7G8) and a strain isolated from a cerebral malaria patient (CM-87) were equally susceptible to treatment with a phosphorothioate oligomer. Inhibition of red cell invasion is primarily responsible for the observed decrease in proliferation as determined by a study of parasite maturation in the presence of a 30-mer nonsense phosphorothioate oligodeoxynucleotide.
High-Lift Systems on Commercial Subsonic Airliners (1996)

The early breed of slow commercial airliners did not require high-lift systems because their wing loadings were low and their speed ratios between cruise and low speed (takeoff and landing) were about 2:1. However, even in those days the benefit of high-lift devices was recognized. Simple trailing-edge flaps were in use, not so much to reduce landing speeds, but to provide better glide-slope control without sideslipping the airplane and to improve pilot vision over the nose by reducing attitude during low-speed flight. As commercial-airplane cruise speeds increased with the development of more powerful engines, wing loadings increased and a real need for high-lift devices emerged to keep takeoff and landing speeds within reasonable limits. The high-lift devices of that era were generally trailing-edge flaps. When jet engines matured sufficiently in military service and were introduced commercially, airplane speed capability had to be increased to best take advantage of jet engine characteristics. This speed increase was accomplished by introducing the wing sweep and by further increasing wing loading. Whereas increased wing loading called for higher lift coefficients at low speeds, wing sweep actually decreased wing lift at low speeds. Takeoff and landing speeds increased on early jet airplanes, and, as a consequence, runways worldwide had to be lengthened. There are economical limits to the length of runways; there are safety limits to takeoff and landing speeds; and there are speed limits for tires. So, in order to hold takeoff and landing speeds within reasonable limits, more powerful high-lift devices were required. Wing trailing-edge devices evolved from plain flaps to Fowler flaps with single, double, and even triple slots. Wing leading edges evolved from fixed leading edges to a simple Krueger flap, and from fixed, slotted leading edges to two- and three-position slats and variable-camber (VC) Krueger flaps. The complexity of high-lift systems probably peaked on the Boeing 747, which has a VC Krueger flap and triple-slotted, inboard and outboard trailing-edge flaps. Since then, the tendency in high-lift system development has been to achieve high levels of lift with simpler devices in order to reduce fleet acquisition and maintenance costs. The intent of this paper is to: (1) review available high-lift devices, their functions, and design criteria; (2) appraise high-lift systems presently in service on commercial air liners; (3) present personal study results on high-lift systems; (4) develop a weight and cost model for high-lift systems; and (5) discuss the development tendencies of future high-lift systems.
Synthesis of Ionic Conducting Interpenetrating Polymer Networks. (1986)

Epoxy and ionic conducting poly(ethylene oxide)-salt complexes form inter-penetrating polymer networks. The co-continuity of the two phases are tested independently using mechanical and electrical measurements. Examination of the network using transmission electron microscopy suggests that the size scale of the phases ranges from 0.1 to 0.5 microns.
Determination of the metabolites of Ruda leaf (Ruta graveolens L.) cultivated in Campeche (2023)

Rue (Ruta graveolens L.) is a plant that is found all year round in the State of Campeche, its leaves are used in the Chenes region and in Champotn for headaches, headaches, or rashes.The results obtained from drying in an oven, in aqueous extract at room temperature are: lactones +, alkaloids +, essential oils +, in hot aqueous extract are: alkaloids +, essential oils +, cardiotonic glycosides +, reducing sugars +; in ethanolic extract at room temperature they are: quinones +, flavonoids +, alkaloids +, essential oils +, reducing sugars +; in the hot aqueous extracts they are: phenols +, tannins +, quinones +, flavonoids +, alkaloids +, essential oils +, reducing sugars +.The presence of flavonoids gives it antioxidant action, the primary amino acids share the ability to polymerize with each other to form proteins.With this information, it is achieved that this resource is valued and that those who have it in their yard keep it for the benefits it offers.