gebhart/patrepeval huggingface dataset:
Abstract 2168: Testing the electrical properties of different cell lines using 3DEP reader and compare to TTFields response (2019)

Background: Tumor Treating Fields (TTFields) are an approved anti-neoplastic treatment modality delivered via application of low intensity, intermediate frequency, alternating electric fields. The electrical properties of cells (such as permittivity and conductivity) determine the optimal frequency of TTFields that incurs the highest reduction in cell counts. Currently, there are no predictive markers for determining TTFields response or the optimal frequency to be applied for individual patient. The goal of this study is to determine the correlation between electrical properties of cells and TTFields’s optimal frequency and sensitivity. The 3DEP reader (LabTech) determines the electrical properties of cells, including permittivity and conductivity, by using Dielectrophoresis (DEP) force. DEP is a physical effect that generates a force on polarizable particles experiencing a non-homogeneous electric field and can therefore be used as a technique to analyse the way cells move within electric fields at different frequencies. Methods: The baseline electrical properties (permittivity and conductivity) of 17 cell lines from different tumor types were determined using the 3DEP reader (LabTech). The curves were analyzed using 2-way ANOVA. The optimal TTFields frequency for each cell line was determined by testing the cytotoxic effect of TTFields at various frequencies using the inovitro system. The electrical properties of cells were compared with the optimal TTFields frequency and sensitivity of each cell line. Results: The results demonstrate significant differences (p<0.001) between the lower frequency range of the 3DEP curves that corresponds to membrane capacitance of cells with TTFields optimal frequency of 150kHz (9 cell lines) and cells with TTFields optimal frequency of 200kHz (8 cell lines). Membrane capacitance was also a good predictor for TTFields sensitivity based on the differences in the curves in the low frequency range. Conclusions: The results presented in this study demonstrate that cell membrane capacitance correlates with TTFields optimal frequency and sensitivity. Based on the above, there is a strong rational to further explore the potential of measuring the electrical properties of cells as a predictive marker to help determine which patient will respond better to TTFields and the optimal TTFields frequency to be applied for each patient.Citation Format: Moshe Giladi, Einav Zeevi, Karnit Gotlib, Cornelia Wenger, Ariel Naveh, Zeev Bomzon, Eilon D. Kirson, Uri Weinberg, Adrian Kinzel, Yoram Palti. Testing the electrical properties of different cell lines using 3DEP reader and compare to TTFields response [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2019; 2019 Mar 29-Apr 3; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2019;79(13 Suppl):Abstract nr 2168.
Factors involved in patient choice of oral or vaginal treatment for vulvovaginal candidiasis (2013)

Vulvovaginal candidiasis (VVC) is an extremely common cause of vaginal symptoms in women. Multiple antifungal products are available by either the oral or vaginal route, although no new drugs have become available for two decades. Given the therapeutic equivalence of the antimycotic agents and their routes of administration, the specific drug and formulation selected is entirely arbitrary in relation to final treatment outcome. Nevertheless, multiple factors affecting preference, both practitioner-dependent and patient-dependent, impact on selection of a specific drug and route of administration.
Comparison of Estimated Conductor Costs between a Superconducting Thin-film Fault-current Limiter (FCL) and a Coated-conductor-based Superconducting FCL (2006)

We recently proposed a new design for a high-temperature superconducting thin-film fault-current limiter (FCL), which uses high-resistivity Au-Ag alloy shunt layers instead of the pure gold (or silver) shunt layers conventionally used. Due to the much larger resistance of the Au-Ag alloy layers, the FCL elements withstood very high electric fields (> 40 Vpeak/cm), and realized a very high switching power density, ∼2.0 kVA/cm2. The composition of our FCL element is very simple, and the achieved power density is more than five times higher than conventional devices, which leads to a dramatic reduction in the amount of expensive superconducting thin films required. Similarly, Kinder et al. recently proposed a new coated-conductor-based FCL element, which achieved a relatively high electric field of 2.7 Vpeak/cm. We estimated the cost of our thin-film FCL elements used in a typical 6.6 kV FCL that is introduced in a distributed power supply site. We also estimated the cost of coated conductors used to produce the FCL, and compared the two.
An Externally Pressurized Supersonic Gas Thrust Bearing (2nd Report, Dynamic Characteristics in Supersonic Operations and Static Characteristics in Sonic Operations) (1984)

The dynamic characteristics of a newly proposed supersonic gas thrust bearing are analyzed under supersonic operations. Its static characteristics in sonic operations are also investigated. It is concluded that the load capacity, the stiffness factor, and the damping factor are increased proportionally to the supply pressure in either supersonic or sonic operation range which is determined according to the magnitude of the clearance.
<title>High-power operation of distributed-feedback resonant-periodic-gain surface-emitting lasers</title> (1992)

At any particular wavelength, only the regions in the vicinity of standing-wave maxima canprovide gain required to sustain lasing oscillations, while pumping of regions near the nodesprovides gain for competing longitudinal modes. If the resonator supports only one mode (forexample, in single-mode distributed-feedback edge-emitting lasers), the carriers injected into
Further exploration of the structure-activity relationship of dual soluble epoxide hydrolase/fatty acid amide hydrolase inhibitors (2021)

Fatty acid amide hydrolase (FAAH) is a membrane protein that hydrolyzes endocannabinoids, and its inhibition produces analgesic and anti-inflammatory effects. The soluble epoxide hydrolase (sEH) hydrolyzes epoxyeicosatrienoic acids (EETs) to dihydroxyeicosatetraenoic acids. EETs have anti-inflammatory and inflammation resolving properties, thus inhibition of sEH consequently reduces inflammation. Concurrent inhibition of both enzymes may represent a novel approach in the treatment of chronic pain. Drugs with multiple targets can provide a superior therapeutic effect and a decrease in side effects compared to ligands with single targets. Previously, microwave-assisted methodologies were employed to synthesize libraries of benzothiazole analogs from which high affinity dual inhibitors (e.g. 3, sEH IC<sub>50</sub> = 9.6 nM; FAAH IC<sub>50</sub> = 7 nM) were identified. Here, our structure-activity relationship studies revealed that the 4-phenylthiazole moiety is well tolerated by both enzymes, producing excellent inhibition potencies in the low nanomolar range (e.g. 6o, sEH IC<sub>50</sub> = 2.5 nM; FAAH IC<sub>50</sub> = 9.8 nM). Docking experiments show that the new class of dual inhibitors bind within the catalytic sites of both enzymes. Prediction of several pharmacokinetic/pharmacodynamic properties suggest that these new dual inhibitors are good candidates for further in vivo evaluation. Finally, dual inhibitor 3 was tested in the Formalin Test, a rat model of acute inflammatory pain. The data indicate that 3 produces antinociception against the inflammatory phase of the Formalin Test in vivo and is metabolically stable following intraperitoneal administration in male rats. Further, antinociception produced by 3 is comparable to that of ketoprofen, a traditional nonsteroidal anti-inflammatory drug. The results presented here will help toward the long-term goal of developing novel non-opioid therapeutics for pain management.
Enhanced discrimination of DNA molecules in nanofluidic channels through multiple measurements (2012)

Author Manuscript 2013 March 21.
Model Process for Removal of Caseins from Milk of Transgenic Animals (2001)

We describe a method for selective removal of caseins from milk. The method was developed as a model for transgenic milk processing. Raw cow milk spiked with nonmilk proteins was chosen as the model to resemble transgenic animal milk containing recombinant proteins. The most important elements of the process are (1) "deconstruction" of casein micelles in milk by destroying their Ca(2+) core using a chelating agent (EDTA), thus freeing any protein that might be entrapped in casein aggregates, and (2) "reconstruction" of micelles by providing them with a new Ca(2+) core, thus precipitating them away from the whey proteins, and the protein of interest. Calcium phosphate particles (CAP) were used to reform the disrupted casein micelles. The crystal clear supernatant fraction generated by this method provided >90% recovery and 6- to 13-fold concentration of the desired protein. Product-rich supernatant contained no detectable casein residues, as silver-stained SDS-PAGE and Western blot analyses demonstrated.
GFP-expressing mammalian cells for fast, sensitive, noninvasive cell growth assessment in a kinetic mode (1999)

This study correlates the fluorescent signal from stable recombinant CHO cell lines expressing the green fluorescent protein (GFP) at high levels with biomass or cell number, extending the use of fluorescent proteins to applications and assays where cell growth rates are important. Using a standard fluorometer, growth of these cells can be quantified noninvasively in multiwell plates, and because signals are obtained without preparation, the same culture samples can be measured repeatedly. Even with a small relative change in biomass, the specific growth rate can be determined in a few hours. The dynamics of cell populations can now be studied with high sensitivity, low error rate, and minimum sample preparation. © 1999 John Wiley & Sons, Inc. Biotechnol Bioeng 65: 201–205, 1999.
Facilitated Intranasal Induction of Mucosal and Systemic Immunity to Mutans Streptococcal Glucosyltransferase Peptide Vaccines (2001)

Synthetic peptide vaccines which are derived from functional domains of Streptococcus mutans glucosyltransferases (GTF) have been shown to induce protective immunity in Sprague-Dawley rats after subcutaneous injection in the salivary gland region. Since mucosal induction of salivary immunity would be preferable in humans, we explored methods to induce mucosal antibody in the rat to the GTF peptide vaccines HDS and HDS-GLU after intranasal administration. Several methods of facilitation of the immune response were studied: the incorporation of peptides in bioadhesive poly(D,L-lactide-coglycolide) (PLGA) microparticles, the use of monoepitopic (HDS) or diepitopic (HDS-GLU) peptide constructs, or the use of mucosal adjuvants. Salivary immunoglobulin A (IgA) responses were not detected after intranasal administration of diepitopic HDS-GLU peptide constructs in alum or after incorporation into PLGA microparticles. However, significant primary and secondary salivary IgA and serum IgG antibody responses to HDS were induced in all rats when cholera holotoxin (CT) or a detoxified mutant Escherichia coli heat-labile enterotoxin (R192G LT) were intranasally administered with HDS peptide constructs in PLGA. Coadministration of LT with HDS resulted in predominantly IgG2a responses in the serum, while coadministration with CT resulted in significant IgG1 and IgG2a responses to HDS. Serum IgG antibody, which was induced to the HDS peptide construct by coadministration with these adjuvants, also bound intact mutans streptococcal GTF in an enzyme-linked immunosorbent assay and inhibited its enzymatic activity. Thus, immune responses which are potentially protective for dental caries can be induced to peptide-based GTF vaccines after mucosal administration if combined with the CT or LT R192G mucosal adjuvant.
Throughput-competitive admission control for continuous media databases (1998)

Article Throughput-competitive admission control for continuous media databases Share on Authors: Minos N. Garofalakis University of Wisconsin-Madison University of Wisconsin-MadisonView Profile , Yannis E. Ioannidis Department of Informatics, University of Athens, Hellas Greece and University of Wisconsin-Madison Department of Informatics, University of Athens, Hellas Greece and University of Wisconsin-MadisonView Profile , Banu Özden Bell Laboratories Bell LaboratoriesView Profile , Avi Silberschatz Bell Laboratories Bell LaboratoriesView Profile Authors Info & Claims PODS '98: Proceedings of the seventeenth ACM SIGACT-SIGMOD-SIGART symposium on Principles of database systemsMay 1998 Pages 79–88https://doi.org/10.1145/275487.275497Online:01 May 1998Publication History 3citation244DownloadsMetricsTotal Citations3Total Downloads244Last 12 Months4Last 6 weeks0 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteGet Access
Dual‐Color Fluorescence Cross‐Correlation Spectroscopy Using Single Laser Wavelength Excitation (2004)

Spotlight on interactions: The authors show that single wavelength excitation fluorescence cross-correlation spectroscopy (SW-FCCS) is possible using only one laser beam to excite a combination of labels, in work performed with studied FRET (fluorescence resonance energy transfer) dyes and quantum dots. They demonstrate that equilibrium binding and stoichiometry can easily be determined (see graphic; QR = quantum red dye, BF = fluorescein-labeled biotin). SW-FCCS is a promising tool for high-throughput screening applications as well as for the measurement of molecular interactions.
Synthesis and characterization of sulfonated poly(phthalazinone ether ketone) for proton exchange membrane materials (2003)

Abstract As a novel class of proton exchange membrane materials for use in fuel cells, sulfonated poly(phthalazinone ether ketone)s (SPPEKs) were prepared by the modification of poly(phthalazinone ether ketone). Sulfonation reactions were conducted at room temperature with mixtures of 95–98% concentrated sulfuric acid and 27–33% fuming sulfuric acid with different acid ratios, and SPPEK was obtained with a degree of sulfonation (DS) in the desired range of 0.6–1.2. The presence of sulfonic acid groups in SPPEK was confirmed by Fourier transform infrared analysis, and the DS and structures were characterized by NMR. The introduction of sulfonic groups into the polymer chains increased the glass‐transition temperature above the decomposition temperature and also led to an overall decrease in the decomposition temperature. Membrane films were cast from SPPEK solutions in N,N ‐dimethylacetamide. Water uptakes and swelling ratios of SPPEK membrane films increased with DS, and SPPEKs with DS > 1.23 were water‐soluble at 80 °C. Proton conductivity increased with DS and temperature up to 95 °C, reaching 10 −2 S/cm. © 2003 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 41: 497–507, 2003
Link analysis using time series of web graphs (2007)

Link analysis is a key technology in contemporary web search engines. Most of the previous work on link analysis only used information from one snapshot of web graph. Since commercial search engines crawl the Web periodically, they will naturally obtain time series data of web graphs. The historical information contained in the series of web graphs can be used to improve the performance of link analysis. In this paper, we argue that page importance should be a dynamic quantity, and propose defining page importance as a function of both PageRank of the current web graph and accumulated historical page importance from previous web graphs. Specifically, a novel algorithm named TemporalRank is designed to compute the proposed page importance. We try to use a kinetic model to interpret this page importance and show that it can be regarded as the solution to an ordinary differential equation. Experiments on link analysis using web graph data in five snapshots show that the proposed algorithm can outperform PageRank in many measures, and can effectively filter out newly appeared link spam websites.
Utility-Based Downlink Pilot Assignment In Cell-Free Massive Mimo (2018)

We propose a strategy for orthogonal downlink pilot assignment in cell-free massive MIMO (multiple-input multiple-output) that exploits knowledge of the channel state information, the channel hardening degree at each user, and the mobility conditions for the users. These elements, properly combined together, are used to define a user pilot utility metric, which measures the user's real need of a downlink pilot for efficient data decoding. The proposed strategy consists in assigning orthogonal downlink pilots only to the users having a pilot utility metric exceeding a predetermined threshold. Instead, users that are not assigned with an orthogonal downlink pilot decode the data by using the statistical channel state information. The utility-based approach guarantees higher downlink net sum throughput, better support both for high-speed users and shorter coherent intervals than prior art approaches.
<i>In vivo</i> Tissue Engineering of a Trilayered Leaflet-Shaped Tissue Construct (2020)

<b>Aim:</b> We aimed to develop a leaflet-shaped trilayered tissue construct mimicking the morphology of native heart valve leaflets. <b>Materials & methods:</b> Electrospinning and <i>in vivo</i> tissue engineering methods were employed. <b>Results:</b> We developed leaflet-shaped microfibrous scaffolds, each with circumferentially, randomly and radially oriented three layers mimicking the trilayered, oriented structure of native leaflets. After 3 months <i>in vivo</i> tissue engineering with the scaffolds, the generated leaflet-shaped tissue constructs had a trilayered structure mimicking the orientations of native heart valve leaflets. Presence of collagen, glycosaminoglycans and elastin seen in native leaflets was observed in the engineered tissue constructs. <b>Conclusion:</b> Trilayered, oriented fibrous scaffolds brought the orientations of the infiltrated cells and their produced extracellular matrix proteins into the constructs.
Auditory space modeling and simulation via orthogonal expansion and generalized spline model (1992)

A two-stage model that establishes a mathematical representation of auditory space is developed. The first stage of the model consists of a low-dimensional subspace representation for the free-field-to-eardrum transfer functions (FETF’s). The bases of this subspace are complex-valued eigentransfer functions (EF’s) obtained from the Karhunen–Loeve expansion of the measured FETF’s covariance matrix. Each FETF is represented as a weighted sum of the EF’s. The second stage of the model is a functional representation for the weights, termed spatial transformation characteristic functions (STCF’s), applied to the EF’s. The STCF’s are functions of azimuth and elevation. A generalized spline model is fitted to each STCF derived from measurements. The spline model filters out noise and permits interpolation of the STCF between measured directions. The FETF’s for an arbitrary direction is synthesized by weighting the EF’s with the smoothed and interpolated STCF’s. Using FETF’s sampled uniformly over the upper 3/4 sphere for one KEMAR ear, it is shown that 99.9% of the energy in the measured FETF’s is contained in a 16-dimensional subspace. The relative average mean-square error between 2320 measured and simulated FETF’s is found to be less than 0.25%. [Raw data provided by Dept. of Neurophysiology, University of Wisconsin−Madison.]
Multi-model, multi-objective tuning of fixed-structure controllers (2014)

We present a new technique for tuning arbitrary linear control structures against multiple plant models, multiple H <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> and H <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">∞</sub> performance requirements, and additional constraints on open-loop stability and closed-loop pole locations. Our approach relies on non-smooth optimization and strikes a good balance between flexibility and effectiveness. Its capabilities are illustrated on two challenging applications.
Anthropomorphic cardiac ultrasound phantom (1989)

A new phantom is described which simulates the human cardiac anatomy for applications in ultrasound imaging, ultrasound Doppler, and color-flow Doppler imaging. The phantom consists of a polymer left ventricle which includes a prosthetic mitral and aortic valve and is connected to a mock circulatory loop. Aerated tap water serves as a blood simulating fluid and ultrasound contrast medium within the circulatory loop. The left ventricle is housed in a Lexan ultrasound visualization chamber which includes ultrasound viewing ports and acoustic absorbers. A piston pump connected to the visualization chamber by a single port pumps degassed water within the chamber which in turn pumps the left ventricle. Real-time ultrasound images and Doppler studies measure flow patterns through the valves and within the left ventricle.
Organs-on-Chips with combined multi-electrode array and transepithelial electrical resistance measurement capabilities (2017)

Here we demonstrate that microfluidic cell culture devices, known as Organs-on-a-Chips can be fabricated with multifunctional, real-time, sensing capabilities by integrating both multi-electrode arrays (MEAs) and electrodes for transepithelial electrical resistance (TEER) measurements into the chips during their fabrication. To prove proof-of-concept, simultaneous measurements of cellular electrical activity and tissue barrier function were carried out in a dual channel, endothelialized, heart-on-a-chip device containing human cardiomyocytes and a channel-separating porous membrane covered with a primary human endothelial cell monolayer. These studies confirmed that the TEER-MEA chip can be used to simultaneously detect dynamic alterations of vascular permeability and cardiac function in the same chip when challenged with the inflammatory stimulus tumor necrosis factor alpha (TNF-α) or the cardiac targeting drug isoproterenol. Thus, this Organ Chip with integrated sensing capability may prove useful for real-time assessment of biological functions, as well as response to therapeutics.
Tracer gas method for measuring leakage through mine stoppings (1978)

The Bureau of Mines has developed a tracer gas method for measuring leakage through permanent mine stoppings. In this method, a temporary brattice is erected in the same entry as the leaking permanent stopping. Leakage was evaluated from concentration measurements of a tracer gas introduced into the space between the stopping and brattice. Stopping leakages ranging from 15 cfm to 265 cfm were simulated in an underground coal mine. Leakage values determined by the new method were within 10% of the known values.
In Vitro Glycosylation Assay for Bacterial Oligosaccharyltransferases (2013)

Oligosaccharyltransferases (OTases) constitute a family of glycosyltransferases that catalyze the transfer of an oligosaccharide from a lipid donor to an acceptor molecule, commonly a protein. These enzymes can transfer a variety of glycan structures, including polysaccharides, to different protein acceptors. Therefore, this property endows the OTases with great biotechnological potential as these enzymes could be applied to produce several glycoconjugates relevant to the pharmaceutical industry. Furthermore, bacterial OTases are thought to be involved in pathogenesis mechanisms. Here we describe how to purify a representative OTase and its protein acceptor and glycan donor to perform in vitro glycosylation studies.