gebhart/patrepeval huggingface dataset:
Mutations in 5-methylcytosine oxidase TET2 and RhoA cooperatively disrupt T cell homeostasis (2017)

Angioimmunoblastic T cell lymphoma (AITL) represents a distinct, aggressive form of peripheral T cell lymphoma with a dismal prognosis. Recent exome sequencing in patients with AITL has revealed the frequent coexistence of somatic mutations in the Rho GTPase RhoA (RhoAG17V) and loss-of-function mutations in the 5-methylcytosine oxidase TET2. Here, we have demonstrated that TET2 loss and RhoAG17V expression in mature murine T cells cooperatively cause abnormal CD4+ T cell proliferation and differentiation by perturbing FoxO1 gene expression, phosphorylation, and subcellular localization, an abnormality that is also detected in human primary AITL tumor samples. Reexpression of FoxO1 attenuated aberrant immune responses induced in mouse models adoptively transferred with T cells and bearing genetic lesions in both TET2 and RhoA. Our findings suggest a mutational cooperativity between epigenetic factors and GTPases in adult CD4+ T cells that may account for immunoinflammatory responses associated with AITL patients.
Einleitung (1993)

ZusammenfassungTechnologie war seit jeher ein Schlüsselelement für die wirtschaftliche und gesellschaftliche Entwicklung. Ganze Zeitabschnitte wurden nach spezifischen Technologien benannt — vom Steinzeitalter über das Dampfzeitalter bis zum Zeitalter des Computers. Aber Technologie ist mehr als Computer und Autos. Techologie ist eine Kombination von Wissen, Fähigkeiten, Techniken und Konzepten. Technologie bedeutet Werkzeuge und Maschinen, Handwerksateliers, Büros und Fabriken; sie bedeutet aber ebenso sehr Organisation, Prozesse und Menschen. Der kulturelle, historische und organisationeile Kontext, in dem Technologie entwickelt und angewendet wird, ist der Schlüssel für erfolgreiche oder misslingende Entwicklung.
A Case Series of Percutaneous Treatment of Non-Union Fractures with Autologous, Culture Expanded, Bone Marrow Derived, Mesenchymal Stem Cells and Platelet Lysate (2011)

Background: Current treatment options for stable non-union fractures represent major clinical challenges, and are a major health issue. Fracture treatment can take many forms, usually requiring bone grafting and/or revisions of the fracture with open reduction and internal fixation (ORIF). Conservative care options such as bone morphogenic proteins and bone stimulators are also available. The purpose of this study was to determine if culture expanded, autologous MSC's injected into non-union fractures under c-Arm fluoroscopy could represent an alternative treatment modality in recalcitrant fracture non-unions. This paper reports on the findings of 6 patients with fracture non-union treated with autologous MSC's.
The soluble form of E-selectin is an asymmetric monomer. Expression, purification, and characterization of the recombinant protein. (1994)

The gene coding for a soluble form of human E-selectin (sE-selectin) has been expressed in Chinese hamster ovary (CHO) cells. Cells seeded into a hollow fiber reactor secreted protein at a level of 160 mg/liter. The protein was purified to > 95% pure and low endotoxin (< 2 ng/mg), using physiological pH and buffers. The amino acid composition and N-terminal sequence were as predicted from the cDNA sequence. HL-60 cells bound to sE-selectin-coated plates in a dose-dependent manner, and this binding could be blocked up to 100% by pretreatment of HL60 cells with sE-selectin. The concentration of sE-selectin required for 50% inhibition was 1 microM. This value puts an upper limit for the affinity of E-selectin for its natural receptor. sE-selectin also inhibited inflammatory migration of neutrophils in a selective fashion. Purified sE-selectin exhibited a broad band of M(r) approximately 75,000 on nonreducing SDS-PAGE. sE-selectin eluted with M(r) approximately 310,000 from size exclusion chromatography at physiological pH and buffers, suggesting an oligomeric state. Matrix-assisted laser-desorption MS gave a molecular weight of 80,000, while the minimum monomer molecular weight from the gene sequence should be 58,571, demonstrating that the monomeric molecule thus expressed had 27% carbohydrate. Equilibrium analytical ultracentrifugation gave an average solution molecular weight of 81,600 (+/- 4,500). Velocity ultracentrifugation gave a sedimentation coefficient of 4.3 S and, from this, an apparent axial ratio of 10.5:1, assuming a prolate ellipsoid of revolution. An analysis of the NMR NOESY spectra of sE-selectin, sialyl-Lewis X, and sE-selectin with sialyl-Lewis X demonstrates that the recombinant protein binds sialyl-Lewis X productively. Hence, in solution, sE-selectin is a functional elongated monomer.
A phase 1 trial of the Fc-engineered CD19 antibody XmAb5574 (MOR00208) demonstrates safety and preliminary efficacy in relapsed CLL (2014)

CD19 is ubiquitously expressed on chronic lymphocytic leukemia (CLL) cells and is therefore an attractive candidate for antibody targeting. XmAb5574 (aka MOR00208) is a novel humanized CD19 monoclonal antibody with an engineered Fc region to enhance Fcγ receptor binding affinity. Here we report results of a first in human phase 1 trial of XmAb5574 in patients with relapsed or refractory CLL. Twenty-seven patients were enrolled to 6 escalating dose levels, with expansion at the highest dose level of 12 mg/kg. Nine doses of XmAb5574 were infused over 8 weeks. No maximal tolerated dose was reached, and the drug was generally well tolerated, with infusion reactions of grades 1 and 2 being the most common toxicities. Grade 3 and 4 toxicities occurred in 5 patients and included neutropenia, thrombocytopenia, increased aspartate aminotransferase, febrile neutropenia, and tumor lysis syndrome. XmAb5574 showed preliminary efficacy, with 18 patients (66.7%) responding by physical examination criteria and laboratory studies, and 8 patients (29.6%) responding by computed tomography criteria. Pharmacokinetics showed a half-life of 14 days with clearance that was not dose-dependent. In conclusion, this phase 1 trial demonstrates safety and preliminary efficacy of a novel Fc-engineered CD19 monoclonal antibody XmAb5574 and justifies movement into the phase 2 setting. This trial was registered at www.clinicaltrials.gov as #NCT01161511.
Solid-state current limiter for power distribution system (1993)

To prevent voltage decrease of distribution systems, the principle and fundamental characteristics of a solid-state current limiter using GTO thyristors were investigated. Basic components of the apparatus were a fast solid-state switch and a current limiting impedance of low resistance in parallel with the switch. Experimental results of the test current limiter showed the fault current was limited successfully, regardless of DC component size. The time from detection of fault occurrence to interruption of the fault current by the solid-state switch was 40 mu s. This time was very short in comparison with that before the fault current reached a large value. Thermal rise of the solid-state switch for conduction was solved by a self-cooling apparatus using a noncombustible cooling liquid. The results indicated that the solid-state current limiter was a valuable protecting device for high fault current distribution systems.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
New and Evolving Concepts in the Imaging and Management of Urolithiasis: Urologists’ Perspective (2010)

Urolithiasis is a universal problem that has become increasingly prevalent in the United States and has a high rate of recurrence. Imaging of urolithiasis has evolved over the years due to technologic advances and a better understanding of the disease process. Computed tomography (CT) has been the investigation of choice for the evaluation of urinary stone disease. The emergence of multidetector CT and the recent introduction of dual-energy CT have further reinforced the superiority of this modality over other imaging techniques in the management of urolithiasis. Multidetector CT is not limited to simply helping make an accurate diagnosis in patients with stone disease; it is also useful in the assessment of stone burden, composition, and fragility, findings that are helpful in determining appropriate treatment strategies. In addition, multidetector CT is a valuable tool in the follow-up of patients after urologic intervention or institution of medical therapy. Familiarity with recent technologic developments will help radiologists meet the growing expectations of urologists in this setting. In addition, radiologists should be aware of the radiation risks inherent in the imaging of patients with urolithiasis and take appropriate measures to minimize this risk and optimize image quality.
The complex structure of GRL0617 and SARS-CoV-2 PLpro reveals a hot spot for antiviral drug discovery (2021)

SARS-CoV-2 is the pathogen responsible for the COVID-19 pandemic. The SARS-CoV-2 papain-like cysteine protease (PLpro) has been implicated in playing important roles in virus maturation, dysregulation of host inflammation, and antiviral immune responses. The multiple functions of PLpro render it a promising drug target. Therefore, we screened a library of approved drugs and also examined available inhibitors against PLpro. Inhibitor GRL0617 showed a promising in vitro IC<sub>50</sub> of 2.1 μM and an effective antiviral inhibition in cell-based assays. The co-crystal structure of SARS-CoV-2 PLpro<sup>C111S</sup> in complex with GRL0617 indicates that GRL0617 is a non-covalent inhibitor and it resides in the ubiquitin-specific proteases (USP) domain of PLpro. NMR data indicate that GRL0617 blocks the binding of ISG15 C-terminus to PLpro. Using truncated ISG15 mutants, we show that the C-terminus of ISG15 plays a dominant role in binding PLpro. Structural analysis reveals that the ISG15 C-terminus binding pocket in PLpro contributes a disproportionately large portion of binding energy, thus this pocket is a hot spot for antiviral drug discovery targeting PLpro.
Development of new SILP catalysts using chitosan as support (2007)

New SILP catalysts based on chitosan-supported ionic liquid were successfully applied to allylic substitution reactions. A high level of activity combined with recyclability and reusability were obtained in the amination reaction. No organic solvent was used. The scope and limitations of the reaction using these new SILP catalysts were estimated.
Self-Assembly of Amphiphilic Perylene−Cyclodextrin Conjugate and Vapor Sensing for Organic Amines (2010)

An asymmetric, amphiphilic perylene bisimide derivative 1 was synthesized by grafting permethyl-β-cyclodextrin at one side and an octadecyl chain at the other side. Its aggregation capability and morphology, which attract intense interest, were carefully examined by combination of UV-vis, NMR, and fluorescence spectroscopy, DLS, XRD, TEM, and SEM. By adjusting the volume ratio of water and methanol, we are able to control the morphology, benefiting from the amphiphilicity of 1. Furthermore, the particular resulting aggregates were employed as solid-state fluorescence sensing for organic amines. An improvement of both selectivity and sensitivity is achieved compared to previous publications.
Use of DOP-PCR for amplification and labeling of BAC DNA for FISH (2011)

Fluorescence in situ hybridization (FISH) is a powerful molecular cytogenetic method that permits rapid detection of specific chromosomal rearrangements. It is based on the hybridization of fluorescent labeled probes to metaphase chromosomes or interphase nuclei. The DNA probes commonly are generated from cloned sources such as bacterial artificial chromosomes (BACs). The major disadvantage of this approach is that it requires laborious and time-consuming work. We used a degenerate oligonucleotide primed polymerase chain reaction (DOP-PCR) for both amplification and labeling of very small amounts of purified BAC DNA for FISH. The DOP-PCR reaction was processed in two steps: pre-amplification followed by simultaneous amplification and labeling of BAC DNA. The DOP-PCR probes obtained provided good hybridization signals and low background. Thus, DOP-PCR can be used to produce unlimited quantities of FISH probes with decreased cost and labor.
Thermal Energy Storage for a Dish Stirling Concentrated Solar Power System (2013)

A phase change salt Thermal Energy Storage (TES) device has been designed and built for use with a Stirling engine in a concentrated solar power system. A eutectic blend of NaCl and NaF was used for the latent heat energy storage medium, with a melt temperature of 680C and energy storage capacity of 43 MJ (12 kW*h). A sodium heat pipe integrated the heat transport function from the solar receiver surface to the Stirling heater head and the molten salt canister during charge mode and from the salt canister to the heater head during discharge mode. The heat pipe was an elaborate hybrid structure with interconnected vapor chamber regions at the receiver and heater head, with extension tubes into the salt canister. Multiple extension tubes were used to maximize contact area with the salt. A sintered powder wick was used to accommodate the high fluxes at the receiver and multilayer screen wick throughout the rest of the heat pipe. The assembly was mostly Inconel 625, with Haynes 230 at the receiver. The layout of the multi-chambered heat pipe required careful analysis and design. A CFD tool was used to evaluate wick heat transport capability in a variety of orientations. Fabrication of the welded heat pipe & salt canister assembly required careful sequencing. In order to suppress corrosion, processing of the salt charge was done in a manner that minimized residual moisture. A basic heat pipe functional test was done before delivery of the system to the customer for thorough performance testing (which is described in a related paper).
A legal safety concept for highly automated driving on highways (2011)

This paper discusses the design of an Advanced Driver Assistance System (ADAS) that ensures safety when traffic rules are respected by all road users. This concept, referred to as legal safety, is proposed as a basis that permits human and automated drivers to share the road infrastructure. It is illustrated for a Highly Automated driving System with speed keeping, distance keeping and lane changing functionalities on highways (HAS). The requirements legal safety places upon HAS components are presented, with a special focus on the co-pilot which calculates a safe trajectory for the vehicle based on perception of lanes and traffic signs and prediction of the trajectories of objects in the environment. A lane coordinate system is proposed as a powerful reference for the trajectory calculations of the co-pilot. The system controls the vehicle and communicates with the driver, according to an automation mode scheme inspired by the horse-rider metaphor (H-metaphor).
Improved BP Neural Network and Its Application in Ship-Based Navigation System (2009)

Poor environment of ship-based navigation system usually leads to decrease in navigation precision and even leads to divergence. BP neural network is introduced to solve this problem. According to characteristics of the navigation system, conventional BP algorithm is improved. And the improved algorithm is used in the ship-based navigation system. The simulation results show that the algorithm can overcome the problem of the navigation system, and ensure the normal operation of the filter.
Analysis of the Australian and Canadian Governments’ Aboriginal Policies (2011)

The Australian and Canadian Aboriginal communities are strikingly similar vis-à-vis traditional lifestyles, social structures and histories of victimization during colonization (Morrisette, McKenzie, & Morrisette, 2010). A cultural context, then, means “an emphasis on historical reconstruction based on an understanding of colonialism to alter the stereotypes and false beliefs given to Aboriginal people, and on culture and traditions as restorative to an individual's identity and self-esteem” (Morrisette et al., 2010, p. 98). At present, both communities suffer similar problems of unemployment, alcoholism, drug abuse, sex abuse, mental illness, suicide and racism. In this paper we explore the contribution of Australian Government policies to the lack of progress in Australia.
PIV Measurements of Flow in Recirculation Cavities at the Inlet of a Centrifugal Compressor (2012)

,Because of the necessity of mass flow range as large as possible on turbocharger compressors to match different engine regimes, the surge and stall phenomena, that limit the operational range at low mass flow, need to be controlled. Numerous active and passive devices exist in order to reduce or suppress their occurrence. A method that has been proven to work and is commonly used consists of recirculation passages that extract high entropy flow from the tip of the blades to reduce blockage in the blades. The compressor used in the study features a ported shroud connected by a slot to the main passage that removes part of the flow near the inducer and mixes it upstream into the inlet. In order to understand the mechanism and assess the flow patterns in the recirculation channels, PIV measurements were carried out in the inlet region. Data was collected along a compressor speedline and allows understanding of the flow in the channels and its interaction with the incoming flow for different operating regimes of the compressor.
Use of conventional PCR and smear microscopy to diagnose pulmonary tuberculosis in the Amazonian rainforest area (2014)

The diagnostic usefulness of Ziehl-Neelsen (ZN)-stained sputum smears combined with conventional polymerase chain reaction (ZN/PCR) to amplify IS6110 region DNA extracted from ZN slides was evaluated. The objective was to verify if this association could improve tuberculosis (TB) diagnosis in patients at remote sites. The study was carried out in 89 patients with culture-confirmed pulmonary TB as defined by the Brazilian Manual for TB Treatment. The participants were recruited in a reference unit for TB treatment in Rondônia, a state in the Amazonian area in northern Brazil. ZN, PCR, and culture performed in the sputum samples from these patients were analyzed in different combinations (i.e., ZN plus PCR and ZN plus culture). The prevalence rates of pulmonary TB in these patients were 32.6 and 28.1% considering culture and ZN/PCR, respectively. The sensitivity and specificity of ZN/PCR were 86 and 93%, respectively. ZN/PCR was able to detect more TB cases than ZN alone. This method could offer a new approach for accurate tuberculosis diagnosis, especially in remote regions of the world where culture is not available.
Minimizing nonspecific phagocytic uptake of biocompatible gold nanoparticles with mixed charged zwitterionic surface modification (2011)

Gold nanoparticles (AuNPs) with excellent resistance to phagocytic uptake by macrophages are achieved via a facile mixed charged zwitterionic self-assembled monolayers (SAMs) surface modification. A series of AuNPs with good dispersity against high salinity, strong resistance to plasma adsorption, persistent stability in cell culture medium and low cytotoxicity are prepared by surface modification with mixed charged SAMs in relatively wide range ratios of oppositely charged thiols. An in vitro study demonstrates that AuNPs modified by mixed charged SAMs effectively minimize the nonspecific cell uptake by either non-phagocytic HUVEC and HepG2 cells or phagocytic RAW 264.7 cells. When different factors that affect cell uptake including incubation time, nanoparticles concentration and incubation mode were evaluated, all results demonstrated that the resistance to phagocytosis by macrophage RAW 264.7 of the zwitterionic AuNPs is even much better than that of the traditional PEGylated AuNPs. A hemolysis assay with human erythrocytes demonstrates the good blood biocompatibility of the zwitterionic AuNPs. The excellent “stealth” properties of the mixed charged AuNPs promise their further biomedical applications both in vitro and in vivo.
Design and reliability analysis of emergency trip system with triple modular redundancy (2009)

According to the high-reliability requirement of emergency trip system (ETS) for steam turbine, a triple modular redundant ETS system is presented in detail in this paper. The system consists of groups of input modules gathering data from sensors, system bus, main controllers running the same application program, and groups of output modules, all of which are configured to triple hardware redundancy with auto-testing and diagnosis function. The parameters synchronization strategy is designed to ensure the real-time and accurate system response, while the adaptable voting mechanism considering auto-testing fault table of system modules is used to ensure the system reliable. By analyzing the system structure and principle, the system reliability model is established. The simulation results and field commissioning proved the triple modular redundant ETS system has a better reliability and stability than the single module system.
Design of Trans-Splicing Adeno-Associated Viral Vectors for Duchenne Muscular Dystrophy Gene Therapy (2008)

The development of trans-splicing vectors opens the door for delivering a large therapeutic gene with adeno-associated viral vectors (AAV). One potential application is to deliver the 6 kb mini-dystrophin gene for Duchenne muscular dystrophy (DMD) gene therapy. However, early attempts have been very disappointing because of low transduction efficiency. We have recently identified mRNA accumulation as a critical barrier for the trans-splicing AAV vectors. This barrier can be overcome by rational selection of the gene splitting site. Here we outline a detailed RNase protection assay-based strategy to determine the optimal gene splitting site for the mini-dystrophin gene. We also provide methods to evaluate transduction efficiency of the mini-dystrophin trans-splicing vectors in mdx mouse, a model for DMD.
Passive low-cost inkjet-printed smart skin sensor for structural health monitoring (2012)

Monitoring fatigue cracking of large engineering structures is a costly and time-intensive process. The authors’ present the first low-cost inkjet-printed patch antenna sensor that can passively detect crack formation, orientation and shape by means of resonant frequency shifts in the two resonant modes of the antenna. For the first time, the effect of non-linear crack shapes on the parallel and perpendicular resonant modes of a patch antenna is quantified with simulation and measurement. This study presents a step towards fully integrated, low-cost, conformal and environmentally friendly smart skins for real-time monitoring of large structures.
Théologies et mystiques de la Grèce hellénistique et de la fin de l’Antiquité (2018)

Catgories d'Aristote : la catgorie du keisthai Dans le cadre d'un projet de traduction commente d'une section du Commentaire de Simplicius aux Catgories d'Aristote, entam depuis plusieurs annes et entr dans une phase d'achvement, une partie du sminaire a t consacre la lecture du chapitre traitant de la catgorie du (Aristote, Cat. 1 b 27 ; 2 a 2-3).