gebhart/patrepeval huggingface dataset:
Antiapoptotic and Oncogenic Potentials of Hepatitis C Virus Are Linked to Interferon Resistance by Viral Repression of the PKR Protein Kinase (1999)

Hepatitis C virus (HCV) is prevalent worldwide and has become a major cause of liver dysfunction and hepatocellular carcinoma. The high prevalence of HCV reflects the persistent nature of infection and the large frequency of cases that resist the current interferon (IFN)-based anti-HCV therapeutic regimens. HCV resistance to IFN has been attributed, in part, to the function of the viral nonstructural 5A (NS5A) protein. NS5A from IFN-resistant strains of HCV can repress the PKR protein kinase, a mediator of the IFN-induced antiviral and apoptotic responses of the host cell and a tumor suppressor. Here we examined the relationship between HCV persistence and resistance to IFN therapy. When expressed in mammalian cells, NS5A from IFN-resistant HCV conferred IFN resistance to vesicular stomatitis virus (VSV), which normally is sensitive to the antiviral actions of IFN. NS5A blocked viral double-stranded RNA (dsRNA)-induced PKR activation and phosphorylation of eIF-2alpha in IFN-treated cells, resulting in high levels of VSV mRNA translation. Mutations within the PKR-binding domain of NS5A restored PKR function and the IFN-induced block to viral mRNA translation. The effects due to NS5A inhibition of PKR were not limited to the rescue of viral mRNA translation but also included a block in PKR-dependent host signaling pathways. Cells expressing NS5A exhibited defective PKR signaling and were refractory to apoptosis induced by exogenous dsRNA. Resistance to apoptosis was attributed to an NS5A-mediated block in eIF-2alpha phosphorylation. Moreover, cells expressing NS5A exhibited a transformed phenotype and formed solid tumors in vivo. Disruption of apoptosis and tumorogenesis required the PKR-binding function of NS5A, demonstrating that these properties may be linked to the IFN-resistant phenotype of HCV.
Improved first wall and limiter surfaces for plasma devices (1981)

For a plasma device, a surface of a first wall or limiter with reduced loss of metal by erosion is provided by forming a monolayer of an alkali or alkaline earth metal on a substrate of a more negative metal. The surface exhibits a reduced loss of metal by erosion and particularly by sputtering and an increased secondary ion/neutral ratio resulting in a greater return of atoms escaping from the surface. In another aspect of the invention, the substrate includes a portion of the second metal and serves to replenish the surface layer with atoms of the second metal. In one process associated with self-generating desired surface, the metals as an alloy are selected to provide a first layer having a high concentration of the second metal in contrast to a very low concentration in the second layer and bulk to result in a surface with a monolayer of the second metal. When the combination of metals results in an intermetallic compound, selective removal of the first metal during an initial bombardment stage provides the surface layer with a predominance of the second metal.
Tumor Susceptibility of <i>Rassf1a</i> Knockout Mice (2005)

The human Ras association domain family 1 (RASSF1) gene is located at 3p21.3 in an area that is believed to harbor at least one important tumor suppressor gene. The two major isoforms of RASSF1, RASSF1A and RASSF1C, are distinguished by alternative NH(2)-terminal exons and the two transcripts initiate in two separate CpG islands. RASSF1A is one of the most frequently inactivated genes described thus far in human solid tumors. Inactivation of RASSF1A most commonly involves methylation of the promoter and CpG island associated with the RASSF1A isoform. In contrast, RASSF1C is almost never inactivated in tumors. Here, we have derived Rassf1a knockout mice in which exon 1-alpha of the Rassf1 gene was deleted, leading to specific loss of Rassf1a but not Rassf1c transcripts. Rassf1a-targeted mice were viable and fertile. Rassf1a(-/-) mice were prone to spontaneous tumorigenesis in advanced age (18-20 months). Whereas only two tumors developed in 48 wild-type mice, six tumors were found in 35 Rassf1a(+/-) mice (P < 0.05) and thirteen tumors were found in 41 Rassf1a(-/-) mice (P < 0.001). The tumors in Rassf1a-targeted mice included lung adenomas, lymphomas, and one breast adenocarcinoma. Rassf1a(-/-) and wild-type mice were treated with two chemical carcinogens, benzo(a)pyrene and urethane, to induce skin tumors and lung tumors, respectively. Rassf1a(-/-) and Rassf1a(+/-) mice showed increased tumor multiplicity and tumor size relative to control animals. The data are consistent with the tumor-suppressive role of Rassf1a, which may explain its frequent epigenetic inactivation in human tumors.
Isobar Suppression by Photodetachment in a Gas-Filled RF Quadrupole Ion Guide (2006)

A novel technique based on selective non-resonant laser photodetachment in a radio frequency quadrupole ion guide is demonstrated for efficient suppression of isobaric contaminants in negative ion beams. The use of the quadrupole ion guide substantially increases the interaction time of the ions with the laser, significantly increasing the efficiency of the photodetachment process. In a proof–of-principle experiment, we achieved 95% suppression of <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">59</sup> Co <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-</sup> ions by photodetachment while under identical conditions only 10% of <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">58</sup> Ni <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-</sup> ions were neutralized.
Transport of the lipophilic analog minocycline differs from that of tetracycline in susceptible and resistant Escherichia coli strains (1982)

Plasmids which specify resistance to tetracycline offer much less resistance to its more lipophilic analog, minocycline. Resistance to minocycline varies for different plasmids. In the case of plasmid R222 (bearing the class B tetracycline resistance determinant on Tn10), minocycline resistance is comparatively high (10 microgram/ml, or 6% of the tetracycline resistance level). For plasmid pIP7 (bearing the class A determinant), minocycline resistance is only 1% of the tetracycline resistance level. To understand the basis for these differences, we compared the transport of the two tetracyclines by susceptible cells and by resistant cells. Uptake of minocycline by susceptible cells was 10 to 20 times more rapid than uptake of tetracycline and occurred largely via an energy-dependent route. This host-mediated energy-dependent uptake of both analogs was still present in tetracycline-resistant cells. In resistant cells, the same plasmid-mediated active efflux system previously described for tetracycline also exported minocycline. The 15-fold greater susceptibility of tetracycline-resistant R222-bearing cells to minocycline as compared with tetracycline could be explained at least in part by the more rapid influx of minocycline, which more easily overcame the efflux system. The particularly low minocycline resistance offered by pIP7 was due to a weak efflux for minocycline, 10-fold less effective than that mediated by R222. The rate-limiting step for uptake of both analogs appeared to be the outer membrane. That the lipophilic minocycline should cross this membrane more rapidly than tetracycline stands in contrast with other studies which show the outer membrane to be a barrier for entry of lipophilic substances.
Fiber-laser-based frequency comb with a tunable repetition rate (2004)

A phase-locked, self-referenced frequency comb generated by a mode-locked fiber soliton laser with a tunable repetition rate is presented. The spacing of the frequency comb is set by the laser's repetition rate, which can be scanned from 49.3 MHz to 50.1 MHz while one tooth of the comb is held phase-locked to a stable RF source. This variable repetitionrate frequency comb should be useful for wavelength and length metrology, synchronization of different fiber laser-based frequency combs, and the generation of precise swept wavelength sources.
Initial Results of a Variable Speed Knee Controller for Walking with a Powered Knee and Ankle Prosthesis (2018)

Powered knee and ankle prostheses can potentially improve the mobility and function of their users, but determining the best way to control the prosthesis is difficult. Controllers that vary knee swing speed have been shown to restore gait symmetry with a powered knee and ankle prosthesis. This study's goal was to combine an existing variable speed knee swing controller with an existing impedance stance controller to determine if comfortable walking with variable cadence can be achieved and if the control method transitions would be noticeable to the user. The knee swing trajectory and duration was varied based on user walking speed as a function of the previous stance phase duration. Four individuals with unilateral transfemoral amputations were fit with a powered knee and ankle prosthesis. After 30-45 minutes of practice walking with the variable knee swing controller, subjects performed a variable speed walk test, a steady state walk test, and a 10-meter walk test. A GAITRite mat was used to collect spatial and temporal walking parameters during the 10-meter walk test. Results showed that subjects could control prosthetic knee swing duration and kinematics by modifying their walking speed. Subjects were able to comfortably transition between speeds and achieve mean (SD) comfortable and fast speeds of 1.10 (0.05) and 1.51 (0.05) m/s, respectively for a 10-meter walk test. This study's contribution is to show that a variable speed knee controller can be combined with an impedance-based controller while maintaining the functionality of both controllers and to provide gait mechanics for amputee powered gait that can be used towards future studies of controller development and prosthesis design.
The C‐type lectin DC‐SIGN (CD209) is an antigen‐uptake receptor for <i>Candida albicans</i> on dendritic cells (2003)

Dendritic cells (DC) that express the type II C-type lectin DC-SIGN (CD209) are located in the submucosa of tissues, where they mediate HIV-1 entry. Interestingly, the pathogen Candida albicans, the major cause of hospital-acquired fungal infections, penetrates at similar submucosal sites. Here we demonstrate that DC-SIGN is able to bind C. albicans both in DC-SIGN-transfected cell lines and in human monocyte-derived DC. The binding was shown to be time- as well as concentration-dependent, and live as well as heat-inactivated C. albicans were bound to the same extent. Moreover, in immature DC, DC-SIGN was able to internalize C. albicans in specific DC-SIGN-enriched vesicles, distinct from those containing the mannose receptor, the other known C. albicans receptor expressed by DC. Together, these results demonstrate that DC-SIGN is an exquisite pathogen-uptake receptor that captures not only viruses but also fungi.
ChemInform Abstract: Electrodeposition of Crystalline and Photoactive Silicon Directly from Silicon Dioxide Nanoparticles in Molten CaCl<sub>2</sub>. (2013)

Abstract Pure, polycrystalline, and photoactive silicon is directly obtained from SiO 2 nanoparticles by electrodeposition in molten CaCl 2 on a silver electrode (quartz tube, 850 °C).
Automatic Retinal Oximetry (2006)

The retinal oximeter is reliable, easy to use, and sensitive to changes in SO(2) when concentration of O(2) in inhaled air is changed.
Immunohistochemical characterization of two monoclonal antibodies, P25.48 and P25.91, which define a new prostate-specific antigen. (1988)

We have generated two new mouse monoclonal antibodies against prostate cancer. P25.48 (IgG3) and P25.91 (IgG2a) were derived from a fusion using fresh prostate cancer cells as the immunogen. Initial screening was performed by indirect immunofluorescence on frozen tissue sections of prostate cancer specimens. The specificity analysis was performed by indirect immunoperoxidase on frozen sections of normal tissues and benign and malignant prostate tissues. P25.48 and P25.91 did not react with any benign prostatic tissues (0 of 17), but reacted with a subset of the malignant prostatic tissues. Five specimens of well-differentiated carcinoma were tested and did not react with P25.48 or with P25.91. Of 16 higher grade specimens, nine reacted with both P25.48 and P25.91, one reacted with P25.48 only, and one reacted with P25.91 only. In most positive cases, the reactivity was heterogenous. P25.48 and P25.91 showed a very restricted pattern of reactivity in nonprostatic tissues. Of 50 normal specimens, only one breast specimen showed some reactivity. None of the nine fetal tissues or of the 15 malignant tissues tested reacted with these monoclonal antibodies. The pattern of reactivity of P25.48 and P25.91 suggests that they recognize the same antigen. This antigen is selectively expressed by malignant prostatic epithelium. In addition, it appears to be distinct from all other previously described prostate cancer-associated antigens.
High Speed Low Swing Dynamic Circuits with Multiple Supply and Threshold Voltages (2006)

A new low voltage swing circuit technique based on a dual threshold voltage CMOS technology is presented in this paper for simultaneously reducing active and standby mode power consumption and enhancing evaluation speed and noise immunity in domino logic circuits. The proposed circuit technique modifies both the upper and lower boundaries of the voltage swing at the dynamic node. Meanwhile, full voltage swing signals are maintained at inputs and outputs for robust and high speed operation. Power supply, ground, and threshold voltages are simultaneously optimized to minimize the power-delay product (PDP). The proposed technique reduces the PDP by up to 51.9% as compared to standard full-swing circuits in a 45nm CMOS technology. The active mode power consumption is reduced by up to 40.4% due to lower switching power required to charge/discharge the dynamic node. Furthermore, the evaluation speed and noise immunity are enhanced by up to 19.4% and 39.1%, respectively, as compared to standard full-swing circuits. The proposed low swing technique also reduces the idle mode leakage power consumption by up to 84.2% in the high fan-in domino gates.
Single-step gas phase synthesis of stable iron aluminide nanoparticles with soft magnetic properties (2014)

Soft magnetic alloys at the nanoscale level have long generated a vivid interest as candidate materials for technological and biomedical purposes. Consequently, controlling the structure of bimetallic nanoparticles in order to optimize their magnetic properties, such as high magnetization and low coercivity, can significantly boost their potential for related applications. However, traditional synthesis methods stumble upon the long standing challenge of developing true nanoalloys with effective control over morphology and stability against oxidation. Herein, we report on a single-step approach to the gas phase synthesis of soft magnetic bimetallic iron aluminide nanoparticles, using a versatile co-sputter inert gas condensation technique. This method allowed for precise morphological control of the particles; they consisted of an alloy iron aluminide crystalline core (DO3 phase) and an alumina shell, which reduced inter-particle interactions and also prevented further oxidation and segregation of the bimetallic core. Remarkably, the as-deposited alloy nanoparticles show interesting soft magnetic properties, in that they combine a high saturation magnetization (170 emu/g) and low coercivity (less than 20 Oe) at room temperature. Additional functionality is tenable by modifying the surface of the particles with a polymer, to ensure their good colloidal dispersion in aqueous environments.
Application of adaptive arrays to communications (1974)

The phase and amplitude weighting of the waveform received at each element of an antenna array can be adaptively controlled in real time to optimize the antenna pattern for a variety of communication links. In this paper we present the results of experiments conducted with an SHF self-phasing array and with an adaptive UHF null-forming array.
Thin film waveguide sensors for chemical detection (1999)

A chemical sensor scheme, based on selective sensing surfaces and highly sensitive integrated optical transduction is presented. Self-assembly techniques are used to covalently attach species selective films onto the surface of silicon nitride waveguides. Exposure to targeted analytes results in selective absorption of these molecules onto the waveguide surface, causing a change in the effective refractive index of the guided modes. These relative changes in effective refractive indices of TM and TE modes are measured using Zeeman interferometry. The measurements demonstrate reversible, real time sensing of volatile organic compounds at ppm level. Improvements in the waveguide design are proposed to further increase the sensor performance.© (1999) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Cost effective realization of nanoscaled interdigitated electrodes (2000)

Nanoscaled interdigitated electrodes (IDEs) are being developed for the realization of miniaturized and highly-sensitive affinity biosensors. Until now, nanoscaled IDEs have been realized on silicon wafers using deep UV lithography or e-beam patterning. However, for many applications in the biochemical field there is a strong need for cheap and/or disposable sensor devices. Therefore, a new, cost effective fabrication method for nanoscaled IDEs, which can also be applied on cheap, micro-moulded plastic substrates, has been developed. The method is based on the directionality of a vacuum evaporation process and omits expensive lithography steps completely. The feasibility of this electrode deposition technique has been proven by realizing IDEs on silicon substrates. Future work is focused on the realization of IDEs on injection moulded plastic substrates.
Multiple reservations and the Oklahoma update (1993)

A multiple reservation approach that allows atomic updates of multiple shared variables and simplifies concurrent and nonblocking codes for managing shared data structures such as queues and linked lists is presented. The method can be implemented as an extension to any cache protocol that grants write access to at most one processor at a time. Performance improvement, automatic restart, and livelock avoidance are discussed. Some sample programs are examined.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Analysis of a Linear Least-Squares Localization Technique in LOS and NLOS Environments (2007)

In this paper, we analyze the localization accuracy of a linear least-squares technique both theoretically and via simulations. Closed-form expressions for the mean square localization errors are derived both for line-of-sight (LOS) and non-line-of-sight (NLOS) scenarios and compared with the Cramer-Rao lower bound (CRLB). Analysis reveals that the localization error tends to increase as the mobile terminal moves away from the reference base-station.
Performance analysis of a type of electrohydrodynamic power generator (1979)

This report develops a detailed analysis of a type of electrohydrodynamic power generator which employs an ejector and a so-called 'fluid flywheel' as essential components. The medium is steam containing electrically charged water droplets. The analysis takes into account the experimentally established facts that the maximum strength of the electrical field that can be sustained at incipient breakdown at the most critical location is proportional to the fluid density at that location. It is shown that as a consequence of this fact, the electrical output can be maxmized by designing the primary jet for an exit Mach number of 0.71. Estimates are made of the pump work required, of mixing losses in the ejector and of friction and secondary flow losses. The mathematical analysis is reduced to a fully non-dimensional form and the key dimensionless parameters that govern performance are clearly identified. A preliminary estimate is made of the numerical values of these parameters and the overall performance of the system is esitmated on this basis. (Author)
Host humoral immune suppression by <i>Clostridioides difficile</i> Toxin A and Toxin B (2022)

Abstract Gram-positive Clostridioides difficile is the greatest cause of nosocomial enteric disease in the US and is associated with a high rate of morbidity and mortality. During C. difficile infection (CDI), two secreted toxins (TcdA and TcdB) drive pathogenesis. Although T-dependent humoral immunity, specifically high affinity TcdA- and TcdB-neutralizing IgG, is the best correlation for protection against CDI recurrence, this response is commonly lacking following CDI in both mice and patients, leading to a high chance of recurrence. We previously showed that in murine CDI, there was a lack of T follicular helper (Tfh) cell differentiation, reduced IgG class-switching, poor toxin neutralization, and lack of resistance to reinfection. Preliminary data also suggested that TcdB inhibited IgG responses following vaccination against C. difficile. We therefore hypothesize that TcdA and/or TcdB suppress C. difficile-specific T-dependent humoral immunity. Following either CDI or intraperitoneal injection with TcdA or TcdB, immune cells from mesenteric lymph nodes were analyzed for changes in gene expression. Changes in dendritic cell maturation markers were observed in the presence of TcdB but not TcdA. Multi-targeting mRNA profiling showed altered expression of antigen presentation and CD4+ T cell activation genes. Only one of the known TcdB cell entry receptors was expressed by immune cells, specifically by CD4+ T cells and B cells. Efforts are underway to measure the impact of TcdA and TcdB treatment on CD4+ T cell differentiation, Tfh proliferation, and T-B cell interactions. Our data indicate that toxins may hamper neutralizing IgG production by affecting multiple aspects of T-dependent humoral immunity. This work was supported by NIH grants AI134719 (M.L.L.) and AI119048 (J.D.B.).
Continuous-wave and high-power pulsed operation of index-coupled distributed feedback quantum cascade laser at λ≈8.5 μm (1998)

High performance index-coupled quantum cascade distributed feedback (QC-DFB) lasers operating at λ≈8.5 μm are reported. Reliable dynamic single-mode emission with a side mode suppression ratio ⩾30 dB is obtained. The continuous single-mode tuning range is 140 nm. In pulsed operation a record high peak output power of 60 mW at 300 K is achieved. We further report on the first continuous-wave QC-DFB lasers. These devices display an output power of 10 mW at 120 K.
Quantitative analysis of wound healing (1995)

In this article, we initially review several problems associated with the design and interpretation of certain types of experiments currently used to study wound healing, drawing attention to the fact that applications for standard statistical techniques in the analysis of the experimental results are often of limited value. We then argue that, because of the special nature of wound healing data, curve fitting of empirical model equations can often provide a convenient way to summarize treatment effects with large data sets. The various ways in which this technique could be used to facilitate the interpretation of experimental wound healing results are then explored. To illustrate this approach, we then took several wound healing experiments and introduced possible models that could be used, paying particular attention to simple equations with the smallest possible number of parameters. For each equation, the way that the parameters of the model could be interpreted with regard to the biologic effects represented is given. Examples are given to show the application of each model discussed theoretically in the interpretation of some typical experimental data sets.