gebhart/patrepeval huggingface dataset:
Monoclonal Antibodies Against Human Endothelin-Converting Enzyme-1 (1998)

Endothelin-converting enzyme-1 (ECE-1) is a membrane-bound metalloprotease which specifically converts the inactive precursor big-endothelin-1 (big ET-1) to the vasoactive endothelin-1 (ET-1). Six different mouse hybridoma cell lines have been generated secreting monoclonal antibodies specific to human ECE-1. These antibodies have been proven useful in a fast and efficient one-step purification of membrane-bound ECE-1 as well as of artificial soluble ECE-1 by immunoaffinity chromatography. The antibodies are suitable for a quantification of ECE-1 in solution by a sandwich-ELISA and for the immunohistochemical detection of ECE-1 in the cell membrane.
Hepatorenal Index as an Accurate, Simple, and Effective Tool in Screening for Steatosis (2012)

The hepatorenal index is a simple, reliable, and cost-effective screening tool for identifying patients who should not undergo liver biopsy for evaluation of steatosis.
Using RESTful web-services and cloud computing to create next generation mobile applications (2009)

In this paper we will examine the architectural considerations of creating next generation mobile applications using Cloud Computing and RESTful Web Services. With the advent of multimodal smart mobile devices like the iPhone, connected applications can be created that far exceed traditional mobile device capabilities. Combining the context that can be ascertained from the sensors on the smart mobile device with the ability to offload processing capabilities, storage, and security to cloud computing over any one of the available network modes via RESTful web-services, has allowed us to enter a powerful new era of mobile consumer computing. To best leverage this we need to consider the capabilities and constraints of these architectures. Some of these are traditional trade-offs from distributed computing such as a web-services request frequency vs. payload size. Others are completely new - for instance, determining which network type we are on for bandwidth considerations, federated identity limitations on mobile platforms, and application approval.
Electro-optic probing of RF signals in submicrometreMMIC devices (1995)

A high resolution electro-optic measurement system for probing RF electric field distributions in submicrometre MMIC devices is presented. As an example, field distributions in an interdigital structure are displayed, revealing a spatial resolution of much less than 0.5 µm. This feature makes electro-optic probing a unique technique for circuit- and also device-internal measurements of electrical signals combining high bandwidth and noninvasiveness with submicrometre resolution.
Efficacy and Safety of Sipjeondaebo‐Tang for Anorexia in Patients with Cancer: A Pilot, Randomized, Double‐Blind, Placebo‐Controlled Trial (2017)

This trial is registered with ClinicalTrials.gov NCT02468141.
Evolution of domain–peptide interactions to coadapt specificity and affinity to functional diversity (2016)

Evolution of complexity in eukaryotic proteomes has arisen, in part, through emergence of modular independently folded domains mediating protein interactions via binding to short linear peptides in proteins. Over 30 years, structural properties and sequence preferences of these peptides have been extensively characterized. Less successful, however, were efforts to establish relationships between physicochemical properties and functions of domain-peptide interactions. To our knowledge, we have devised the first strategy to exhaustively explore the binding specificity of protein domain-peptide interactions. We applied the strategy to SH3 domains to determine the properties of their binding peptides starting from various experimental data. The strategy identified the majority (∼70%) of experimentally determined SH3 binding sites. We discovered mutual relationships among binding specificity, binding affinity, and structural properties and evolution of linear peptides. Remarkably, we found that these properties are also related to functional diversity, defined by depth of proteins within hierarchies of gene ontologies. Our results revealed that linear peptides evolved to coadapt specificity and affinity to functional diversity of domain-peptide interactions. Thus, domain-peptide interactions follow human-constructed gene ontologies, which suggest that our understanding of biological process hierarchies reflect the way chemical and thermodynamic properties of linear peptides and their interaction networks, in general, have evolved.
Induction of programmed cell death in ErbB2/HER2-expressing cancer cells by targeted delivery of apoptosis-inducing factor (2009)

Apoptosis-inducing factor (AIF) is a mitochondrial flavoprotein with NADH oxidase activity that has a vital function in healthy cells but is also an important mediator of caspase-independent programmed cell death in stressed and damaged cells. Here, we generated a truncated AIF derivative (AIF(Delta100)) that lacks the mitochondrial import signal of the protein. Bacterially expressed AIF(Delta100) was functionally active and induced cell death on microinjection into Vero cells accompanied by clear signs of apoptosis. For specific targeting to tumor cells, AIF(Delta100) was genetically fused to the scFv(FRP5) antibody fragment that recognizes the ErbB2 (HER2) receptor tyrosine kinase frequently overexpressed in many human cancers. Recombinant scFv(FRP5)-AIF(Delta100) (5-AIF(Delta100)) protein and a similar scFv(FRP5)-ETA(252-366)-AIF(Delta100) (5-E-AIF(Delta100)) molecule harboring in addition the nontoxic translocation domain of Pseudomonas exotoxin A as an endosome escape function displayed binding to ErbB2-expressing cells followed by protein internalization and accumulation in intracellular vesicles. In the presence of the endosomolytic reagent chloroquine 5-E-AIF(Delta100) but not the similar 5-AIF(Delta100) protein displayed potent cell killing activity, which was strictly dependent on the expression of ErbB2 on the target cell surface. Our results show that recombinant AIF specifically targeted to human cancer cells and delivered into the cytosol has potent cell killing activity, suggesting this molecule as an effector function suitable for the development of humanized immunotoxin-like molecules.
Synthesis and Biological Evaluation of Dihydromotuporamine Derivatives in Cells Containing Active Polyamine Transporters (2005)

Dihydromotuporamine C (4) and its 4,4-triamine analogue (5) were synthesized in good yield using ring-closing metathesis (RCM) methods. Comparison of their biological activities (Ki determinations in L1210 cells and IC50 determinations in L1210, CHO, and CHO-MG cells) revealed that the motuporamine derivatives do not use the polyamine transporter (PAT) for cellular entry. Bioevaluation of a N1-(anthracen-9-ylmethyl)-N1-(ethyl)homospermidine control (7) revealed that the presence of a N1 tertiary amine center imparted a significant reduction in the PAT affinity of the polyamine conjugate and abolished its PAT-targeting selectivity.
Handling concept drifts in incremental learning with support vector machines (1999)

With the increase in the size of real-world databases, there is an ever-increasing need to scale up inductive learning algorithms.
Sensory Evaluation of Mixtures of Maltitol or Aspartame, Sucrose and an Orange Aroma (1998)

The suitability of Beidler's mixture equation for mixtures of sucrose and maltitol as well as for mixtures of sucrose and aspartame was examined in the presence of an orange aroma. The mean scores for the attribute sweet remained constant for each combination of sucrose and maltitol and for each combination of sucrose and aspartame. Therefore, Beidler's mixture equation can be used to choose combinations of sucrose and maltitol and of sucrose and aspartame giving the same sweetness. Quantitative descriptive analysis of different solutions indicated that the flavour profiles of sucrose and maltitol did not differ significantly at a constant concentration of orange aroma. However, flavour profiles of solutions with increasing aspartame concentrations (but constant aroma levels) showed significantly higher scores for the attributes sour, chemical and aftertaste. Addition of orange aroma provided the different solutions with a more distinct flavour. The mean scores for the attributes orange, sour, fruity and aftertaste increased significantly for most of the sucrose-maltitol mixtures. This effect of orange aroma was even more pronounced in solutions containing combinations of sucrose and aspartame. Further comments on the attribute aftertaste showed similar terms for the different solutions, the most often mentioned being orange, sour, fruity and chemical for solutions containing the orange aroma. The aftertaste of solutions containing relatively more aspartame was mainly described as sweet and chemical.
Processing of thin SU-8 films (2008)

This paper summarizes the results of the process optimization for SU-8 films with thicknesses ⩽5 µm. The influence of soft-bake conditions, exposure dose and post-exposure-bake parameters on residual film stress, structural stability and lithographic resolution was investigated. Conventionally, the SU-8 is soft-baked after spin coating to remove the solvent. After the exposure, a post-exposure bake at a high temperature TPEB ⩾ 90 °C is required to cross-link the resist. However, for thin SU-8 films this often results in cracking or delamination due to residual film stress. The approach of the process optimization is to keep a considerable amount of the solvent in the SU-8 before exposure to facilitate photo-acid diffusion and to increase the mobility of the monomers. The experiments demonstrate that a replacement of the soft-bake by a short solvent evaporation time at ambient temperature allows cross-linking of the thin SU-8 films even at a low TPEB = 50 °C. Fourier-transform infrared spectroscopy is used to confirm the increased cross-linking density. The low thermal stress due to the reduced TPEB and the improved structural stability result in crack-free structures and solve the issue of delamination. The knowledge of the influence of different processing parameters on the responses allows the design of optimized processes for thin SU-8 films depending on the specific application.
Oitenta anos de pertença no Ser e no Tempo: uma homenagem de “Ética e Filosofia Política”a Martin Heidegger (2007)

O Programa de Pos-Graduacao em Ciencia da Religiao, na sua area de concentracao em Filosofia da Religiao, atraves do Grupo de Pesquisa em Heidegger, coordenado pelo nosso querido Professor Dr. Paulo Afonso Araujo, vem-nos brindar com a publiacacao de seis artigos sobre um dos maiores pensadores do seculo XX, para comemorar os oitenta anos de “O Ser e o Tempo”.
A smart map representation for autonomous vehicle navigation (2015)

Autonomous vehicle technology has attracted many attention in recent years. However, this technology is still challenging in urban areas because of the complicated environments. A highly detailed map for autonomous vehicle is proposed in this paper. This map representation consists of three basic traffic elements: roads, lanes and lane markings. It can represent both the geometrical and topological information of the traffic environment. A highly detailed map database containing these three elements is generated using ArcGIS based on a LIDAR high precision map. Then, a two-class path planning including road class planning and lane class is executed on this map. The map representation proposed in this paper provides an efficient way for intelligent vehicle to pass an intersection in a safe and reasonable way.
High-Pressure Hydrogen Sulfide Experiments: How Did Our Safety Measures and Hazard Control Work during a Failure Event? (2020)

Hydrogen sulfide (H2S) is a hazardous, colorless, flammable gas with a distinct rotten-egg smell at low concentration. Exposure to a concentration greater than 500 ppm of H2S can result in irreversible health problems and death within minutes. Because of these hazards, operations such as oil and gas processing and sewage treatment that handle or produce H2S and/or sour gas require effective and well-designed hazard controls, as well as state-of-the-art gas monitoring/detection mechanisms for the safety of workers and the public. Laboratories studying H2S for improved understanding must also develop and continually improve upon lab-specific safety standards with unique detection systems. In this study, we discuss various H2S detection methods and hazard control strategies. Also, we share our experience regarding a leak that occurred as a result of the failure of a perfluoroelastomer O-ring seal on a small stirred autoclave vessel used for studying H2S hydrate dissociation/formation conditions in our laboratory, and discuss how our emergency response plan was activated to mitigate the risk of exposure to the researchers and public.
Ohmic contact formation mechanism of Ta∕Al∕Mo∕Au and Ti∕Al∕Mo∕Au metallizations on AlGaN∕GaN HEMTs (2005)

A comparative study of Ta∕Al∕Mo∕Au and Ti∕Al∕Mo∕Au metallizations for AlGaN∕GaN high electron mobility transistors is presented. By the optimization of surface treatment schemes and annealing temperature, contact resistances of 0.172 and 0.228Ωmm, and specific contact resistivities of 2.96×10−7 and 1.09×10−6Ωcm2 were obtained for Ti∕Al∕Mo∕Au and Ta∕Al∕Mo∕Au, respectively. Auger electron spectroscopy (AES), x-ray diffraction (XRD), and transmission electron microscopy (TEM) were utilized to study microstructural changes occurring in the metallization layers as a result of heat treatment. Results indicate dynamic systems of severe intermixing between atoms from the metallization layers and epilayers resulting in changes in surface morphology, as determined by atomic force microscopy (AFM). The formation of intermetallics and interfacial compounds was observed. The combined effect of surface treatment and annealing-induced reaction is proposed as the mechanisms for low-resistance Ohmic contact formation.
Heterospecific expression of misrepair-enhancing activity of mucAB in Escherichia coli and Bacillus subtilis (1991)

Enterobacterial plasmid genes mucAB, which possess error-prone repair activity, were cloned and sequenced independently of a sequence previously determined (K.L. Perry, S.J. Elledge, B.B. Mitchell, L. Marsh, and G.C. Walker, Proc. Natl. Acad. Sci. USA 82:4331-4335, 1985). The survival- and mutation-enhancing activities of mucAB ligated to the MLSr promoter of a Bacillus subtilis plasmid in the shuttle vector pTE22R were expressed in B. subtilis as well as in Escherichia coli after mutagenic treatment. mucAB fragments with 5' deletions of various lengths up to the base sequence encoding Ala-26-Gly-27, the putative RecA-mediated cleavage site of the MucA protein, showed mutation-enhancing activity for noninducible lexA3 E. coli when ligated to the MLSr promoter in frame. This activity was lost by extending the deletion downstream. The formations of MucA and MucB proteins in B. subtilis and E. coli were demonstrated by Western blot (immunoblot) analysis. MucA cleavage in Rec+ B. subtilis was observed only after treatment with an alkylating agent and was not observed in RecA- and RecE- strains, whereas in E. coli cleavage was observed in Rec+ cells after treatment with either mitomycin C or an alkylating agent but was not detected in RecA- cells. Common activity of B. subtilis Rec and E. coli RecA in the induction of mutants is suggested.
Quality Assessment and Prediction of Philippine Mangoes: A Convolutional Neural Network Approach (2019)

Philippines is one of the world’s leading exporter of mangoes. The country produces many varieties of mangoes, one of which is the ‘Carabao’ mango. Several metric tons of mangoes are produced, and these have to be checked for defects before entering the market. With recent advances in technology, it has become efficient and relatively easy to use for these applications. The objective of this paper is to present a non-destructive method to check the quality of mangoes using computer vision (CV) and convolutional neural network (CNN) with a minimal number of samples. An experimental setup was created to simulate a production line. A webcam was used for capturing images of the mangoes, while a mini computer was used for controlling the peripherals. As basis for categorizing the mangoes as either good or bad, the Philippine National Standard (PNS) for mangoes was used. A basic background subtraction algorithm was used to extract the mango’s image. With these extracted images, a 2-category network was trained, and the achieved classification accuracy was 97.21%. The goal of having a high accuracy in classifying mangoes was achieved. There are multiple paths to explore in the future, including additional feature extraction methods, different neural networks, and hardware improvements, in order to speed up the sorting process. Moreover, it may be necessary to be able to identify mangoes with only slight defects to be used for other products, such as dried mangoes, to reduce product wastage.
Noncatalytic Electrodes for Solid‐Electrolyte Oxygen Sensors (1980)

The catalytic effects of various electrode materials on nonequilibrium oxygen measurements using solid‐electrolyte oxygen sensors have been investigated. Catalytically active electrodes can perturb the measurement of oxygen in nonequilibrium mixtures of oxygen and combustible gases. This paper reports the development of noncatalytic electrodes for accurate measurement of the free oxygen content in nonequilibrium gas mixtures containing and , , , or . The effects of Pt, Au, Ag, Ag deposited on Pt, and S‐ or Pb‐poisoned Pt electrodes on the measurement of oxygen in nonequilibrium gas mixtures were determined. These studies were made using solid‐electrolyte oxygen sensors of a new internal‐reference design as well as with traditional air‐reference sensors. Pt electrodes are catalytic in all of the gas mixtures studied. Both Ag electrodes and Ag vapor deposited on Pt were found to be noncatalytic to oxidation while Au electrodes were only slightly catalytic. Pt electrodes poisoned by S introduced as were also noncatalytic to oxidation until the sulfur desorbed at ∼800°C. The above electrodes were sufficiently catalytic to oxidation that they could not be used for quantitative nonequilibrium oxygen measurements when is present. Pt electrodes poisoned by Pb (introduced as ) produced the least perturbation of the nonequilibrium mixtures of oxygen with , , , or . The Pb‐poisoned Pt electrodes allow quantitative measurement of the bulk nonequilibrium oxygen concentrations at temperatures above 500°–550°C, depending on the combustible gas present. At lower temperatures, even the Pb‐poisoned electrodes are catalytic. This suggests that Pb may poison the platinum activity by decreasing the heat of adsorption of adsorbed reaction species. Sulfur‐ or Pb‐poisoned Pt or Pt with a thin layer (<200Å) of Ag had no adverse effect on the electrochemical pumping capabilities of Pt. Ag and Au electrodes performed poorly as electrochemical pump electrodes. The capability of solid‐electrolyte oxygen sensors to measure the partial pressure of gas phase oxygen at the electrode surface was used to investigate the catalytic reaction mechanism of oxidation on Pt electrodes partially poisoned by Pb. The results are best fit by an Eley‐Rideal mechanism where adsorbed reacts with gas phase . However, the data are not sufficiently definitive to rule out the Langmuir‐Hinshelwood mechanism where adsorbed O and react on the surface. The methods used demonstrate that solid‐electrolyte oxygen sensors can be useful experimental tools for identifying catalytic mechanisms.
Human <i>β</i> -Glucuronidase: Structure, Function, and Application in Enzyme Replacement Therapy (2013)

Lysosomal storage diseases occur due to incomplete metabolic degradation of macromolecules by various hydrolytic enzymes in the lysosome. Despite structural differences, most of the lysosomal enzymes share many common features including a lysosomal targeting motif and phosphotransferase recognition sites. β-Glucuronidase (GUSB) is an important lysosomal enzyme involved in the degradation of glucuronate-containing glycosaminoglycan. The deficiency of GUSB causes mucopolysaccharidosis type VII (MPSVII), leading to lysosomal storage in the brain. GUSB is a well-studied protein for its expression, sequence, structure, and function. The purpose of this review is to summarize our current understanding of sequence, structure, function, and evolution of GUSB and its lysosomal enzyme targeting. Enzyme replacement therapy reported for this protein is also discussed.
PDO’s Proactive Approach to Solving a Zonal Isolation Challenge in HarweelHP Wells Using Swell Packers (2006)

The Harweel Cluster in Southern Oman is PDO’s first EOR Project. It is being developed in a phased manner using miscible gas injection. Reservoir simulations have been carried out to show that a significant increase in ultimate recovery can be achieved by successfully isolating gas (and water) if it breaks through prematurely to producing wells. In order for the additional reserves to be obtained, zonal isolation is required over the reservoir section. To date, isolation has proved to be inadequate using conventional cementing technology and techniques.A trial was therefore carried out to determine the effectiveness of using oil swellable elastomers (
Evaluation of IBIS modelling techniques for signal integrity simulations without and with package parasitics (2010)

Input/Output Buffer Information Specification (IBIS) models are widely used in signal integrity analysis because of their ability to protect proprietary information and to reduce simulation time when compared to full SPICE simulations. Generation of IBIS models with I/V and V/T data from a full SPICE model of a typical digital buffer without and with package parasitics is investigated in this paper. Several different IBIS model generation strategies to incorporate package effects are validated with the full SPICE model in order to provide a suitable approach. In addition, the accuracy of IBIS simulations in HSPICE and ADS is investigated.
Studies on the a-acetylation of d-valerolactone and 1-caprolactone (2003)

Abstract—The synthetic approach to a-acetylated d-valero- (7a) and 1-caprolactone (7b) is reported. While 7a was isolated in 21 % yield from the respective iodoester 5a by an alkylation sequence involving transesterification and Finkelstein reaction, 7b was not obtained from 5b but the dimer 8. Also transesterification and olefin ring closing metathesis (RCM) failed to prepare 7b. RCM resulted in the dimeric lactone 10, showing that the formation of 14-membered rings is favored over that of seven-membered rings. A Mukaiyama-type Claisen reaction finally gave a-acetyl lactones 7a and 7b in practically useful quantity of about 10 g (62 % yield): starting lactones 13a,b were converted to silylenolethers 14a,b, which were acetylated with acetic anhydride in presence of the Lewis acid catalyst TiCl4. However, acetylation depends on the addition sequence of starting materials: if the mixture of Ac2O/TiCl4 is added to 14b, lactone 7b can be further converted to give bis-oxepanonyl ethanols 15a,b. Both compounds 15 were characterized by X-ray crystallography and NMR. q 2003
Specific Nucleic Acid Detection Using Photophysical Properties of Quantum Dot Probes (2009)

In the present study, we report a novel separation-free method to detect and quantify avian influenza virus A (H5N1) nucleic acid without amplification, based on the alteration of photophysical parameters of quantum dot (QD) probes after hybridization with specific complementary target DNA. The target DNA was quantified in a custom-made portable device by simultaneously measuring lifetime and quenching of the QD probes. QD probes (25-mer) showed a 30% lifetime reduction and 40% fluorescence quenching when hybridized with complementary 25-mer target DNA. In comparison with a conventional QD-based assay, this assay provides a simple quantitation of nucleic acids with a single labeling step.