gebhart/patrepeval huggingface dataset:
Three-Dimensional Nanoprinting <i>via</i> Scanning Probe Lithography-Delivered Layer-by-Layer Deposition (2016)

Three-dimensional (3D) printing has been a very active area of research and development due to its capability to produce 3D objects by design. Miniaturization and improvement of spatial resolution are major challenges in current 3D printing technology development. This work reports advances in miniaturizing 3D printing to the nanometer scale using scanning probe microscopy in conjunction with local material delivery. Using polyelectrolyte polymers and complexes, we have demonstrated the concept of layer-by-layer nanoprinting by design. Nanometer precision is achieved in all three dimensions, as well as in interlayer registry. The approach enables production of designed functional 3D materials with nanometer resolution and, as such, creates a platform for conducting scientific research in designed 3D nanoenvironments as well. In doing so, it enables production of nanomaterials and scaffolds for photonics devices, biomedicine, and tissue engineering.
Discovery of vascular Rho kinase (ROCK) inhibitory peptides (2019)

Rho-activated kinases (ROCKs) regulate many cellular functions such as proliferation, migration, and smooth muscle contractility, but they are also associated with pathogenesis of many human diseases such as heart failure and hypertension. We used phage display libraries to identify inhibitory polypeptides that bind to the ROCK1 catalytic domain, but do not compete with the ATP-binding pocket, by screening in the presence of high ATP concentrations (1 mM). Peptide7, a promising ROCK inhibitory peptide for both ROCK isoforms, measured at 1.45 ± 0.28 µM for ROCK1 (1–553) and 5.15 ± 1.15 µM for ROCK2. Peptide7 reduced cellular migration in wound healing assays. The binding epitope on ROCK1 was mapped to the flexible activation loop within the catalytic domain. Peptide alanine scanning mutants helped identify critical amino acids to generate optimized Peptide22. This compact ROCK inhibitor facilitated vascular relaxation, blocked neovascularization of endothelial cells, and inhibited MLC phosphatase phosphorylation. Our novel ROCK peptide inhibitors may provide potential treatment of hypertension and PAH progression. Impact statement Rho-activated kinases, known as ROCK(s), are significant signaling components in cells that lead alterations in cellular function. The central role of ROCK in smooth muscle cellular homeostasis makes it an important therapeutic target. Small molecule kinase inhibitors target enzyme active site competing for ATP binding. Although effective, ATP binding active sites are similar among very different kinases, and many small molecule inhibitors suffer from non-specific inactivation which as therapeutics can lead to substantial side effects. Here, we designed experiments to identify ROCK inhibitors that do not target ATP binding, rather develop peptides that inhibit ROCK in the presence of ATP. We identified a peptide that binds the activation loop of the enzyme and effectively inhibits activity. This will allow a development of a new class of drugs with exquisite specificity for the ROCK kinases and potentially revolutionize treatment of high blood pressure, cardiac hypertrophy, and many more diseases.
Physicochemical basis for the rapid time‐action of Lys<sup>B28</sup>Pro<sup>B29</sup>‐insulin: Dissociation of a protein‐ligand complex (1996)

The rate-limiting step for the absorption of insulin solutions after subcutaneous injection is considered to be the dissociation of self-associated hexamers to monomers. To accelerate this absorption process, insulin analogues have been designed that possess full biological activity and yet have greatly diminished tendencies to self-associate. Sedimentation velocity and static light scattering results show that the presence of zinc and phenolic ligands (m-cresol and/or phenol) cause one such insulin analogue, LysB28ProB29-human insulin (LysPro), to associate into a hexameric complex. Most importantly, this ligand-bound hexamer retains its rapid-acting pharmacokinetics and pharmacodynamics. The dissociation of the stabilized hexameric analogue has been studied in vitro using static light scattering as well as in vivo using a female pig pharmacodynamic model. Retention of rapid time-action is hypothesized to be due to altered subunit packing within the hexamer. Evidence for modified monomer-monomer interactions has been observed in the X-ray crystal structure of a zinc LysPro hexamer (Ciszak E et al., 1995, Structure 3:615-622). The solution state behavior of LysPro, reported here, has been interpreted with respect to the crystal structure results. In addition, the phenolic ligand binding differences between LysPro and insulin have been compared using isothermal titrating calorimetry and visible absorption spectroscopy of cobalt-containing hexamers. These studies establish that rapid-acting insulin analogues of this type can be stabilized in solution via the formation of hexamer complexes with altered dissociation properties.
Polarization enhanced visual surveillance techniques (2004)

Visual surveillance relies on visible light to convey information about the environment. An important but less utilized characteristic of light in surveillance is its polarization due to the transverse wave nature of electromagnetic waves. Although the predominant natural light source, the sun light, appear to be unpolarized, the light that actually enters a surveillance camera often has biased polarization distribution due to scattering and reflection by the environment. These differently biased polarization patterns thus carry important information about the environment, such as material type, object shape ...etc. With proper optical setup and computer vision analysis, polarization information can be captured and used to enhance traditional polarization-insensitive visual surveillance techniques. This work presents polarization based optical and computational techniques to improve target detection, to perform novel optical navigation, and to help resolve ambiguities in traditional visual sensors.
Quantitative Peptidomics Study Reveals That a Wound-Induced Peptide from PR-1 Regulates Immune Signaling in Tomato (2014)

Many important cell-to-cell communication events in multicellular organisms are mediated by peptides, but only a few peptides have been identified in plants. In an attempt to address the difficulties in identifying plant signaling peptides, we developed a novel peptidomics approach and used this approach to discover defense signaling peptides in plants. In addition to the canonical peptide systemin, several novel peptides were confidently identified in tomato (Solanum lycopersicum) and quantified to be induced by both wounding and methyl jasmonate (MeJA). A wounding or wounding plus MeJA-induced peptide derived from the pathogenesis-related protein 1 (PR-1) family was found to induce significant antipathogen and minor antiherbivore responses in tomato. This study highlights a role for PR-1 in immune signaling and suggests the potential application of plant endogenous peptides in efforts to defeat biological threats in crop production. As PR-1 is highly conserved across many organisms and the putative peptide from At-PR1 was also found to be bioactive in Arabidopsis thaliana, our results suggest that this peptide may be useful for enhancing resistance to stress in other plant species.
Robust Bioinformatics Recognition with VLSI Biochip Microsystem (2006)

A microsystem architecture for real-time, on-site, robust bioinformatic patterns recognition and analysis has been proposed. This system is compatible with on-chip DNA analysis means such as polymerase chain reaction (PCR) amplification. A corresponding novel artificial neural network (ANN) learning algorithm using new sigmoid-logarithmic transfer function based on error backpropagation (EBP) algorithm is invented. Our results show the trained new ANN can recognize low fluorescence patterns better than the conventional sigmoidal ANN does. A differential logarithmic imaging chip is designed for calculating logarithm of relative intensities of fluorescence signals. The single-rail logarithmic circuit and a prototype ANN chip are designed, fabricated and characterized
Directly-deposited blocking filters for high-performance silicon x-ray detectors (2016)

Silicon X-ray detectors often require blocking filters to mitigate noise and out-of-band signal from UV and visible backgrounds. Such filters must be thin to minimize X-ray absorption, so direct deposition of filter material on the detector entrance surface is an attractive approach to fabrication of robust filters. On the other hand, the soft (E < 1 keV) X-ray spectral resolution of the detector is sensitive to the charge collection efficiency in the immediate vicinity of its entrance surface, so it is important that any filter layer is deposited without disturbing the electric field distribution there. We have successfully deposited aluminum blocking filters, ranging in thickness from 70 to 220nm, on back-illuminated CCD X-ray detectors passivated by means of molecular beam epitaxy. Here we report measurements showing that directly deposited filters have little or no effect on soft X-ray spectral resolution. We also find that in applications requiring very large optical density (> OD 6) care must be taken to prevent light from entering the sides and mounting surfaces of the detector. Our methods have been used to deposit filters on the detectors of the REXIS instrument scheduled to fly on OSIRIS-ReX later this year.
The Personal Assessment Tool: A System Providing Environmental Feedback to Users of Shared Printers for Providing Environmental Feedback (2010)

To face ongoing global warming issues and in general to promote sustainable development, a number of tools have been developed that help people to assess the impact of their behavior on the environment. In this paper we present the Personal Assessment Tool, a system that observes print behavior and aggregates information in ways meant to promote more conscious use of the shared printing resources.
On Wavelength-Routed Networks With Reversible Wavelength Channels (2013)

We observe that the performance of wavelength- routed (WR) networks often suffers from asymmetric traffic and network traffic patterns differing from the original design plans. WR networks with a fixed number of channels in a given transmission direction are inflexible. Therefore, we propose reversible wavelength channels. Like a reversible lane in highway systems, a reversible wavelength channel has the flexibility of its transmission direction being configurable at the setup of a lightpath. So far reversible wavelength channels have not been discussed in WR networks even though we observe that most of the required technologies are already available. In this paper, we discuss all the required technologies for implementing reversible wavelength channels in WR networks. We demonstrate that reversible wavelength channel can provide significant performance improvement for WR networks when the traffic is asymmetric. Even if the traffic is symmetric, we also have nontrivial performance improvement with reversible wavelength channels, i.e., the blocking performance of WR networks with reversible wavelength channels will be similar to that of normal WR networks with doubling the number of fibers per link. Different implementation approaches for reversible wavelength channels are discussed. Among them, the performance of the reversible waveband approach is discussed in detail.
From Peptide to Non-Peptide. 2. The de Novo Design of Potent, Non-peptidal Inhibitors of Platelet Aggregation Based on a Benzodiazepinedione Scaffold (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTFrom Peptide to Non-Peptide. 2. The de Novo Design of Potent, Non-peptidal Inhibitors of Platelet Aggregation Based on a Benzodiazepinedione ScaffoldRobert S. McDowell, Brent K. Blackburn, Thomas R. Gadek, Lawrence R. McGee, Thomas Rawson, Mark E. Reynolds, Kirk D. Robarge, Todd C. Somers, Eugene D. Thorsett, and Cite this: J. Am. Chem. Soc. 1994, 116, 12, 5077–5083Publication Date (Print):June 1, 1994Publication History Published online1 May 2002Published inissue 1 June 1994https://pubs.acs.org/doi/10.1021/ja00091a008https://doi.org/10.1021/ja00091a008research-articleACS PublicationsRequest reuse permissionsArticle Views493Altmetric-Citations97LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (2)»Supporting Information Supporting Information Get e-Alerts
A Portable Diagnostic Device for Cardiac Magnetic Field Mapping (2016)

In this paper we present a portable magnetocardiography device. The focus of this development was delivering a rapid assessment of chest pain in an emergency department. The aim was therefore to produce an inexpensive device that could be rapidly deployed in a noisy unshielded ward environment. We found that induction coil magnetometers with a coil design optimized for magnetic field mapping possess sufficient sensitivity ($104fT/\sqrt{Hz}$ noise floor at 10Hz) and response ($813fT/μV$ at 10Hz) for cycle averaged magnetocardiography and are able to measure depolarisation signals in an unshielded environment. We were unable to observe repolarisation signals to a reasonable fidelity. We present the design of the induction coil sensor array and signal processing routine along with data demonstrating performance in a hospital environment.
Hybrid Reward Architecture for Reinforcement Learning (2017)

One of the main challenges in reinforcement learning (RL) is generalisation. In typical deep RL methods this is achieved by approximating the optimal value function with a low-dimensional representation using a deep network. While this approach works well in many domains, in domains where the optimal value function cannot easily be reduced to a low-dimensional representation, learning can be very slow and unstable. This paper contributes towards tackling such challenging domains, by proposing a new method, called Hybrid Reward Architecture (HRA). HRA takes as input a decomposed reward function and learns a separate value function for each component reward function. Because each component typically only depends on a subset of all features, the corresponding value function can be approximated more easily by a low-dimensional representation, enabling more effective learning. We demonstrate HRA on a toy-problem and the Atari game Ms. Pac-Man, where HRA achieves above-human performance.
<title>Imaging System For Correlating Fluorescence Cell Measurements In Flow</title> (1977)

A high sensitivity camera and imaging system are employed in a special purpose flow cytofluorometer for extraction of fluorescence images of cells and objects in flow. Each image is uniquely correlated with a fluorescence profile or "slit-scan" contour of the cell also derived in flow. Contours are computer analyzed to extract low resolution morphologic information. Optical considerations for imaging in flow and results obtained from the imaging system are presented.
Optical Radio-Frequency Phase Measurement With an Internal-State Rydberg Atom Interferometer (2022)

We realize and model an internal-state Rydberg atom interferometer for measurement of phase and intensity of radio-frequency (rf) electromagnetic waves. A phase reference is supplied to the atoms via a modulated laser beam, enabling atomic measurement of the rf wave's phase without a rf reference wave. The rf and optical fields give rise to closed interferometric loops within the atoms' internal Hilbert space. In our experiment, we construct interferometric loops in the state space ${6{P}_{3/2},90{S}_{1/2},91{S}_{1/2},90{P}_{3/2}}$ of cesium and employ them to measure phase and intensity of a 5-GHz rf wave in a room-temperature vapor cell. Electromagnetically induced transparency on the $6{S}_{1/2}$ to $6{P}_{3/2}$ transition serves as an all-optical interferometer probe. The rf phase is measured over a range of $\ensuremath{\pi}$, and a sensitivity of 2 mrad is achieved. Measurements of rf phase and amplitude at submillimeter optical spatial resolution are demonstrated.
Realizing Wireless Communication Through Software-Defined HyperSurface Environments (2018)

Wireless communication environments are unaware of the ongoing data exchange efforts within them. Moreover, their effect on the communication quality is intractable in all but the simplest cases. The present work proposes a new paradigm, where indoor scattering becomes software-defined and, subsequently, optimizable across wide frequency ranges. Moreover, the controlled scattering can surpass natural behavior, exemplary overriding Snell's law, reflecting waves towards any custom angle (including negative ones). Thus, path loss and multi-path fading effects can be controlled and mitigated. The core technology of this new paradigm are metasurfaces, planar artificial structures whose effect on impinging electromagnetic waves is fully defined by their macro-structure. The present study contributes the software-programmable wireless environment model, consisting of several HyperSurface tiles controlled by a central, environment configuration server. HyperSurfaces are a novel class of metasurfaces whose structure and, hence, electromagnetic behavior can be altered and controlled via a software interface. Multiple networked tiles coat indoor objects, allowing fine-grained, customizable reflection, absorption or polarization overall. A central server calculates and deploys the optimal electromagnetic interaction per tile, to the benefit of communicating devices. Realistic simulations using full 3D ray-tracing demonstrate the groundbreaking potential of the proposed approach in 2.4GHz and 60GHz frequencies.
Solution Heat Treatment and Cold Die Quenching in Forming AA 6xxx Sheet Components: Feasibility Study (2005)

To overcome the major problems in forming aluminium sheet components, such as springback, low formability and microstructure variation a novel process is proposed in this paper. That is combined Solution Heat Treatment (SHT) hot stamping followed by cold die quenching. To determine the feasibility of such process a series of thermal-mechanical tests have been designed and carried out on aluminium alloy AA6082. Three aspects of the forming process are investigated and represented in the paper. The first is to investigate the effects of SHT proportions on the mechanical properties of the material. The second is the effects of quenching rates on the mechanical properties after SHT. The third is the effect of predeformation after the SHT and the quenching rate on the mechanical properties of the formed parts. Summaries are given for each aspect of the study. These tests are to investigate the effects of Solution Heat Treatment time proportion. Variables are also introduced during the cold die quenching, including clearance between the testpiece and dies as well as the applied load. Finally the relationship between quench rate and predeformation is investigated.
Wideband IMD3 suppression through negative baseband impedance synthesis (2021)

Abstract This article presents a power amplifier (PA) linearisation approach based on synthesising a negative impedance termination at the baseband frequency. Negative baseband termination has been previously shown to suppress intermodulation distortion (IMD) products in power amplifiers. Here, this effect is synthesised using a passive feedback network between the drain and gate bias paths of the PA, so that the IMD is suppressed without an increase in the system complexity. The design targets IMD3 suppression at the PA's 3‐dB compression point (P3dB), enabling linear operation into compression, therefore, resulting in improved device utilisation and efficiency. Based on simulation of the feedback response over a 1‐200 MHz frequency range, a network topology is designed that provides both the bias structure and the appropriate transfer function for IMD3 suppression, demonstrating the first reported practical structure to realize this behaviour over a wide range of baseband frequencies. Two different transfer functions are implemented and compared in performance to the nominal design case with standard baseband terminations. Due to the feedback nature of the approach, stability is also addressed. The 2.14‐GHz proof‐of‐concept prototype with feedback exhibits up to 9.5 dB suppression of the two‐tone IMD3 over 10‐150 MHz tone spacing, without reducing the CW performance of the PA.
Structural elements of a pH-sensitive inhibitor binding site in NMDA receptors (2019)

Abstract Context-dependent inhibition of N -methyl-D-aspartate (NMDA) receptors has important therapeutic implications for the treatment of neurological diseases that are associated with altered neuronal firing and signaling. This is especially true in stroke, where the proton concentration in the afflicted area can increase by an order of magnitude. A class of allosteric inhibitors, the 93-series, shows greater potency against GluN1-GluN2B NMDA receptors in such low pH environments, allowing targeted therapy only within the ischemic region. Here we map the 93-series compound binding site in the GluN1-GluN2B NMDA receptor amino terminal domain and show that the interaction of the N-alkyl group with a hydrophobic cage of the binding site is critical for pH-dependent inhibition. Mutation of residues in the hydrophobic cage alters pH-dependent potency, and remarkably, can convert inhibitors into potentiators. Our study provides a foundation for the development of highly specific neuroprotective compounds for the treatment of neurological diseases.
A new type single-phase spindle motor for HDD and DVD (1999)

The cogging torque and dynamic performance of a newly designed single phase brushless DC motor have been investigated in this paper. The single phase brushless spindle motor is usually applied for cooling fan, pump and blower before the performance is improved by the reengineering process. The stator configuration and the drive circuit have been remodeled in order to meet the requirements of the spindle motor used in the DVD and HDD applications. The cogging torque is reduced to 18 g-cm by the salient pole reshaping. An automatic phase adjuster is also introduced to improve the performance of the dynamic characteristics of a single phase spindle motor in this paper. According to the test results, the maximum speed could be increased from 6500 rpm to 12000 rpm and the efficiency from 22% to 38%. A single phase motor has been demonstrated successfully in HDD and DVD applications by reshaping for the salient pole and phase adjusted automatically with the rotation speed.