gebhart/patrepeval huggingface dataset:
The role of two conserved amino acids, glutamine 90 and asparagine 137, in <i>O</i><sup>6</sup>-methylguanine-DNA methyltransferase stability, activity and substrate specificity (1994)

To assess the possibility that two conserved amino acids (glutamine 90 and asparagine 137) in O6-methylguanine-DNA methyltransferase (MGMT) are involved in protein-substrate contact and/or discrimination between favored and non-favored substrates, families of proteins mutant at these two sites were expressed in alkyltransferase-deficient bacteria and analyzed for stability, ability to repair O6-methylguanine (MG)-containing DNA, and ability to differentially repair a preferred (MG-containing DNA) versus a non-preferred (free base MG) substrate. All seven proteins mutant at glutamine 90 (except a proline mutant) were stable in bacteria and repaired MG-containing DNA (> 50% of wild-type levels). A representative glutamine 90 mutant protein was not, however, significantly different from the wild-type protein in the preferential repair of MG-containing DNA versus MG free base. Of eight proteins mutant at asparagine 137, only glutamine and serine mutants repaired MG-containing DNA to any degree (8.5% and 0.8% of wild-type respectively) and only the glutamine mutant protein was detectable in bacterial sonicates by Western blot analysis. Alanine and leucine mutant alkyltransferases, inactive and unstable as non-fusion proteins, could, however, be stably expressed in bacteria as glutathione S-transferase fusion proteins, although the proteins were still inactive in repair. These results suggest that while glutamine 90 has no direct role in MG-DNA methyltransferase-mediated repair or free base/lesioned DNA substrate specificity, asparagine 137 is important in both the stability and activity of the protein and may contribute to the formation or function of the active site of the protein.
Sequencing and de novo analysis of a coral larval transcriptome using 454 GSFlx (2009)

The methods described here for deep sequencing of the transcriptome should be widely applicable to generate catalogs of genes and genetic markers in emerging model organisms. Our data provide the most comprehensive sequence resource currently available for reef-building corals, and include an extensive collection of potential genetic markers for association and population connectivity studies. The characterization of the larval transcriptome for this widely-studied coral will enable research into the biological processes underlying stress responses in corals and evolutionary adaptation to global climate change.
Design and Implementation of a Bluetooth Baseband Module with DMA Interface (2002)

Bluetooth technology is a publicly available specification proposed for Radio Frequency (RF) communication for short-range :1nd point-to-multipoint voice and data transfer. It operates in the 2.4㎓ ISM(Industrial, Scientific and Medical) band and offers the potential for low-cost, broadband wireless access for various mobile and portable devices at range of about 10 meters. In this paper, we describe the structure and the test results of the bluetooth baseband module with direct memory access method we have developed. This module consists of three blocks; link controller, UART interface, and audio CODEC. This module has a bus interface for data communication between this module and main processor and a RF interface for the transmission of bit-stream between this module and RF module. The bus interface includes DMA interface. Compared with the link controller with FIFOs, The module with DMA has a wide difference in size of module and speed of data processing. The small size module supplies lorr cost and various applications. In addition, this supports a firmware upgrade capability through UART. An FPGA and an ASIC implementation of this module, designed as soft If, are tested for file and bit-stream transfers between PCs.
Adaptive bandwidth management for entanglement distribution in quantum networks (2021)

Flexible grid wavelength division multiplexing is a powerful tool in lightwave communications to maximize spectral efficiency. In the emerging field of quantum networking, the need for effective resource provisioning is particularly acute, given the generally lower power levels, higher sensitivity to loss, and inapplicability of optical detection and retransmission. In this letter, we leverage flex grid technology to demonstrate reconfigurable distribution of quantum entanglement in a four-user tabletop network. By adaptively partitioning bandwidth with a single wavelength-selective switch, we successfully equalize two-party coincidence rates that initially differ by over two orders of magnitude. Our scalable approach introduces loss that is fixed with the number of users, offering a practical path for the establishment and management of quality-of-service guarantees in large quantum networks.
Chelation enhanced fluorescence detection of non-metal ions (1989)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTChelation enhanced fluorescence detection of non-metal ionsMichael E. Huston, Engin U. Akkaya, and Anthony W. CzarnikCite this: J. Am. Chem. Soc. 1989, 111, 23, 8735–8737Publication Date (Print):November 1, 1989Publication History Published online1 May 2002Published inissue 1 November 1989https://pubs.acs.org/doi/10.1021/ja00205a034https://doi.org/10.1021/ja00205a034research-articleACS PublicationsRequest reuse permissionsArticle Views1488Altmetric-Citations174LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
[Construction and expression of recombinant adeno-associated virus expressing brain-derived neurotrophic factor]. (2008)

A fusion gene called Ig-BDNF, in which brain-derived neurotrophic factor cDNA fused to the 3' end of signal peptide of Ig coding sequence, was constructed by PCR, digested and subcloned into shuttle plasmid pSNAV to obtain a recombinant plasmid pSNAV-Ig-BDNF. Then the plasmid encoding fusion protein was transfected into 293 cell lines and the stably transfected clones were selected with neomycin. AAV1 containing Ig-BDNF fusion gene vectors were obtained by super-infection by Herpes virus. The resultant adeno-associated virus vectors AAV-Ig-BDNF were confirmed by PCR, Western blotting and a sandwich enzyme-linked immunosorbent assay (ELISA) after infection of 293 cell lines. The results indicated that AAV-Ig-BDNF contained the target gene, and infected cells and produced the fusion protein into the supernatant. The content of BDNF in medium per 5x104 cells over a 24 h incubation period reached 1000 pg/mL. With the help of non-replicative adenovirus during AAV-Ig-BDNF infection, the expression of BDNF increased 7-8 fold, and the enhancement of BDNF gene expression was observed in a concentration-dependent manner. These results suggested that a functional AAV-Ig-BDNF was successfully constructed and it offers basis for further study for gene therapy of neural degeneration diseases.
High-vacuum planar magnetron discharge (1992)

The present article demonstrates the possibility of developing a high-vacuum planar magnetron discharge. Until recently, developed cathodes applying a high electric field and a high magnetic field which cross each other in a discharge space enable the discharge to be sustained at 0.1 Pa. Experimental results of this article show that even at pressures lower than 10−3 Pa the discharge can be sustained. The discharge intensity I/P is proportional to the discharge voltage and depends strongly on the magnetic flux density. It is found that the high-vacuum planar magnetron discharge requires a magnetic flux density higher than 0.1 T and a discharge voltage higher than 2 kV, for its effective sustenance. Sputtering of aluminum by this discharge is performed and the deposition rate 0.2 μm/min is obtained.
Innate Immune Sensing of Modified Vaccinia Virus Ankara (MVA) Is Mediated by TLR2-TLR6, MDA-5 and the NALP3 Inflammasome (2009)

Modified vaccinia virus Ankara (MVA) is an attenuated double-stranded DNA poxvirus currently developed as a vaccine vector against HIV/AIDS. Profiling of the innate immune responses induced by MVA is essential for the design of vaccine vectors and for anticipating potential adverse interactions between naturally acquired and vaccine-induced immune responses. Here we report on innate immune sensing of MVA and cytokine responses in human THP-1 cells, primary human macrophages and mouse bone marrow-derived macrophages (BMDMs). The innate immune responses elicited by MVA in human macrophages were characterized by a robust chemokine production and a fairly weak pro-inflammatory cytokine response. Analyses of the cytokine production profile of macrophages isolated from knockout mice deficient in Toll-like receptors (TLRs) or in the adapter molecules MyD88 and TRIF revealed a critical role for TLR2, TLR6 and MyD88 in the production of IFNbeta-independent chemokines. MVA induced a marked up-regulation of the expression of RIG-I like receptors (RLR) and the IPS-1 adapter (also known as Cardif, MAVS or VISA). Reduced expression of RIG-I, MDA-5 and IPS-1 by shRNAs indicated that sensing of MVA by RLR and production of IFNbeta and IFNbeta-dependent chemokines was controlled by the MDA-5 and IPS-1 pathway in the macrophage. Crosstalk between TLR2-MyD88 and the NALP3 inflammasome was essential for expression and processing of IL-1beta. Transcription of the Il1b gene was markedly impaired in TLR2(-/-) and MyD88(-/-) BMDM, whereas mature and secreted IL-1beta was massively reduced in NALP3(-/-) BMDMs or in human THP-1 macrophages with reduced expression of NALP3, ASC or caspase-1 by shRNAs. Innate immune sensing of MVA and production of chemokines, IFNbeta and IL-1beta by macrophages is mediated by the TLR2-TLR6-MyD88, MDA-5-IPS-1 and NALP3 inflammasome pathways. Delineation of the host response induced by MVA is critical for improving our understanding of poxvirus antiviral escape mechanisms and for designing new MVA vaccine vectors with improved immunogenicity.
A Novel Strategy to Screen Bacillus Calmette-Guérin Protein Antigen Recognized by γδ TCR (2011)

In conclusion, our study provides a novel strategy to identify epitopes or protein antigens for the γδ TCR, and provides a potential means to screen mycobacterial vaccines or candidates for adjuvant.
Adp‐Ribosylation of Guanyl Nucleotide‐Binding Regulatory Proteins by Bacterial Toxins (1988)

Introduction Pertussis Toxin Choleragen (Cholera Toxin) Similarities between Choleragen and Escherichia coli heat-Labile Mono-ADP-Ribosyltransferases in Animal Cells
Clinical and Functional Characterization of <i>TNNT2</i> Mutations Identified in Patients With Dilated Cardiomyopathy (2009)

We conclude that the combination of clinical, pedigree, molecular genetic, and functional data strengthen the interpretation of TNNT2 mutations in DCM.
Mechanistic Studies on Direct Arylation of Pyridine <i>N</i>-Oxide: Evidence for Cooperative Catalysis between Two Distinct Palladium Centers (2012)

Direct arylations of pyridine N-oxide (PyO), a convenient method to prepare 2-arylpyridines, catalyzed by Pd(OAc)(2) and PtBu(3) have been proposed to occur by the generation of a PtBu(3)-ligated arylpalladium acetate complex, (PtBu(3))Pd(Ar)(OAc) (1), and the reaction of this complex with PyO. We provide strong evidence that 1 does not react directly with PyO. Instead, our data imply that the cyclometalated complex [Pd(OAc)(tBu(2)PCMe(2)CH(2))](2), which is generated from the decomposition of 1, reacts with PyO and serves as a catalyst for the reaction of PyO with 1. The reaction of PyO with 1 occurs with an induction period, and the reaction of 1 with excess PyO in the presence of [Pd(OAc)(tBu(2)PCMe(2)CH(2))](2) is zeroth-order in 1. Moreover, the rates of reactions of PyO with bromobenzene catalyzed by [Pd(OAc)(tBu(2)PCMe(2)CH(2))](2) and [Pd(PtBu(3))(2)] depend on the concentration of [Pd(OAc)(tBu(2)PCMe(2)CH(2))](2) but not on the concentration of [Pd(PtBu(3))(2)]. Finally, the reaction of 1 with a model heteroarylpalladium complex containing a cyclometalated phosphine, [(PEt(3))Pd(2-benzothienyl)(tBu(2)PCMe(2)CH(2))], rapidly formed the arylated heterocycle. Together, these data imply that the rate-determining C-H bond cleavage occurs between PyO and the cyclometalated [Pd(OAc)(tBu(2)PCMe(2)CH(2))](2) rather than between PyO and 1. In this case, the resulting heteroarylpalladium complex transfers the heteroaryl group to 1, and C-C bond-formation occurs from (PtBu(3))Pd(Ar)(2-pyridyl oxide). This mechanism proposed for the direct arylation of PyO constitutes an example of C-H bond functionalization in which C-H activation occurs at one metal center and the activated moiety undergoes functionalization after transfer to a second metal center.
A CMOS Compatible Monolithic Fiber Attach Solution with Reliable Performance and Self-alignment (2020)

We report a fiber-attach solution interfacing self-aligned, standard-cleaved fibers to monolithic photonic integrated circuits, fabricated in Globalfoundries 300-mm CMOS production facilities. Statistical yield analysis and reliability assessment were performed to demonstrate the robustness of the proposed solution.
Fatigue-strength prediction of microelectronics solder joints under thermal cyclic loading (1997)

The stress-strain analyses for the solder joints in a thin single outline package (TSOP), a ball grid array (BGA) assembly, and a leadless ceramic chip carrier (LCCC) are carried out to investigate the plastic-creep behavior, and stress relaxation behavior due to the temperature cycling or isothermal cyclic loading. The temperature dependence of plastic behavior (yield stress) and creep behavior (creep properties) are taken into consideration in all numerical analyses. The results of finite element analysis (FEA) show that in an accelerated temperature cycling test, long high-temperature and low-temperature dwell times do not contribute to the increase of the cyclic inelastic equivalent strain range in solder joints (although the creep behavior occurring during the dwell times in an operating condition is important enough to be taken into consideration for estimating the fatigue life of solder joints). Based upon the results of the strain analyses, some efficient testing processes of temperature cycling and isothermal fatigue tests for the microelectronic solder joints are proposed, and the cycling tests are carried out. The experimental results show a good agreement with the analytic results.
A fundus camera dedicated to the screening of diabetic retinopathy in the primary-care physician's office. (2002)

The DigiScope fulfills the instrumental requirements for a practical and cost-effective tool to acquire data needed to identify diabetic patients who must be referred to an eye-care specialist. Widespread screening with the DigiScope may help reduce the risk of vision loss in an estimated 4 million individuals in the United States alone, who currently do not undergo an annual eye examination.
Fabrication additive de pièces en polymères thermoplastiques hautes performances et en polyamide 12 par le procédé de frittage sélectif par laser (2014)

Selective Laser Sintering (SLS) of thermoplastic polymer powders is now widely used as a additive manufacturing technique. Nevertheless, this process is industrially mature for only one family of polymers : the polyamides. To use this process in manufacturing applications that are used above 50 °C, it is necessary to increase the range of useable powders to high temperature resistant families of thermoplastic such as poly(aryl-ether-ketone) or poly(aryl-imide). This study investigates the layer-by-layer additive manufacturing of complex parts by SLS from high temperature resistant thermoplastic powders. Seven polymers powders were selected to study their influences on the process steps and the quality of sintered/melted materials. To do so, morphology of theirs particles, microstructures or tapped and poured density are analysed, and also theirs thermal stabilities, capacities to absorb water or theirs flow abilities. In a second step, a study of the influence of process parameters has been carried out to obtain parts with good material quality, taking into account the evolution of the powder after each cycle of fabrication. Moreover, it is important to use all the possibilities of this process in terms of geometry. That is why a law for the mechanical behaviour of laser sintered polyamide 12 has been determined and implemented in a finite element code (ZeBuLoN®). This law, in its linear and non-linear domain, is representative of the material anisotropy and has been experimentally validated on tensile samples and one aerospace part.
The Inactivation of KlNOT4, a Kluyveromyces lactis Gene Encoding a Component of the CCR4-NOT Complex, Reveals New Regulatory Functions (2005)

We have isolated the KlNOT4 gene of the yeast Kluyveromyces lactis, which encodes a component of the evolutionarily conserved CCR4-NOT complex. We show that inactivation of the gene leads to pleiotropic defects that were differentially suppressed by the NOT4 gene of S. cerevisiae, indicating that these genes have overlapping, but not identical, functions. K. lactis strains lacking Not4p are defective in fermentation and show reduced transcription of glucose transporter and glycolytic genes, which are phenotypes that are not found in the corresponding mutant of S. cerevisiae. We also show that Not4 proteins control the respiratory pathway in both yeasts, although with some differences. They activate transcription of KlACS2 and KlCYC1, but repress KlICL1, ScICL1, ScACS1, and ScCYC1. Altogether, our results indicate that Not4p is a pivotal factor involved in the regulation of carbon metabolism in yeast.
In vivo Degradation of Resin-Dentin Bonds in Humans Over 1 to 3 Years (2000)

The longevity of resin restorations is currently an area of great interest in adhesive dentistry. However, no work has been conducted to investigate the durability of resin-dentin bond structures using human substrate in vivo. The purpose of this study was to investigate the degradation of the resin-dentin bond structures aged in an oral environment for 1, 2, or 3 years. Cavities were prepared in primary molars, and an adhesive resin system (Scotchbond Multi-Purpose) was applied to the cavity. After 1 to 3 years, following the eruption of the succedaneous permanent teeth, the resin-restored teeth were extracted. Immediately after extraction, those teeth were sectioned perpendicular to the adhesive interface and trimmed to produce an hourglass-shaped specimen. Then, a micro-tensile test was performed at a crosshead speed of 1.0 mm/min. The mean bond strengths were statistically compared with one-way ANOVA and Fisher's PLSD test (p < 0.05). Further, all fractured surfaces were observed by SEM, and the area fraction of failure mode was calculated by means of a digital analyzer on SEM photomicrographs. There were significant differences in tensile-bond strength among all 3 groups (p < 0.05), with mean values ranging from 28.3 +/- 11.3 MPa (control), to 15.2 +/- 4.4 MPa (1 to 2 years), to 9.1 +/- 5.1 MPa (2 to 3 years). Moreover, under fractographic analysis, the proportion of demineralized dentin at the fractured surface in specimens aged in an oral environment was greater than that in control specimens. Furthermore, degradation of resin composite and the depletion of collagen fibrils was observed among the specimens aged in an oral environment. Analysis of the results of this study indicated that the degradation of resin-dentin bond structures occurs after aging in the oral cavity.
Influence of eddy current losses in the optimization of linear coreless BLDC motors with PCB windings (2019)

This paper presents a series of numerical models aimed at estimating the main characteristics of linear coreless BLDC motors for the optimal sizing of these latter. The paper concentrates on windings printed on PCB. This kind of winding offers opportunities to significantly improve motors' performance, although it is more subject to eddy current losses than wire windings. Therefore, considering the harmonics contained in the airgap magnetic field, the work especially focus on the development of a 2D FEM model able to estimate these eddy currents in the copper tracks. The objective is to evaluate the eddy current harmonics and to adapt the winding shape and the motor dimensions, accordingly. The final aim of this work is therefore to propose full motor optimizations for various operating points and to observe the evolution of its optimal configuration.
Bayesian Multitask Learning with Latent Hierarchies (2009)

We learn multiple hypotheses for related tasks under a latent hierarchical relationship between tasks. We exploit the intuition that for domain adaptation, we wish to share classifier structure, but for multitask learning, we wish to share covariance structure. Our hierarchical model is seen to subsume several previously proposed multitask learning models and performs well on three distinct real-world data sets.
Ananas : A New Adhoc Network Architectural Scheme (2002)

In this paper, we present a new architecture for adhoc networks : Ananas. Based on the combined use of a classical adhoc routing protocol and virtual adhoc interfaces, Ananas allows a complete support for the IP protocol over adhoc networks, including auto-configuration mechanisms such as the IPv6 one or DHCP. It also provides Internet connectivity and compatibility with Internet services and protocols such as multicast. Finally Ananas addresses problems of scalability and adhoc network partitioning.
Patterning of electroless copper deposition on low temperature co-fired ceramic (2013)

The metallization of low temperature co-fired ceramic (LTCC) is typically done with commercially available silver or gold pastes using conventional screen printing processes. However, for some applications such as substrate integrated waveguides and high performance planar components where the metallization of vertical side walls is required, screen printing is difficult to apply. In such cases, electroless plating of copper can be employed to metallize the fired LTCC, which would also be a promising alternative to the high cost metals such as Ag and Au. In this study, electroless copper plating was combined with KrF excimer laser machining to deposit selective copper patterns on fired LTCC. Using this approach, a functional circuit was fabricated and electrically tested. The mask projection technique of the excimer laser can be used to create complex patterns on LTCC and initial work has been carried out to realize planar passive component layouts, including a capacitor and an inductor. The minimum feature size of the deposited copper that could be achieved in these designs was below 50 μm.