gebhart/patrepeval huggingface dataset:
Non‐uniform assembly of the <i>Bacillus anthracis</i> exosporium and a bottle cap model for spore germination and outgrowth (2007)

Spores of Bacillus anthracis are enclosed by an exosporium composed of a basal layer and an external hair-like nap. The nap is formed by a collagen-like glycoprotein called BclA, while the basal layer contains many different proteins, one of which is a spore-specific alanine racemase (Alr). In this study, we employed fluorescence microscopy and a fluorescently labelled anti-Alr monoclonal antibody (mAb) to examine the distribution of Alr within the exosporium. Binding of the mAb occurred over approximately three-quarters of the exosporium but not in a cap-like region at one end of the spore, indicating the absence or inaccessibility of Alr in this region. We also determined that the cap-like region, or cap, corresponds to the first part of the exosporium assembled within the mother cell during sporulation and the only part of the exosporium assembled in a DeltaexsY mutant strain of B. anthracis. Our results provide the first direct evidence that exosporium assembly is a non-uniform process and suggest that exosporium formation is discontinuous. Finally, we demonstrated that during spore germination and outgrowth, the outgrowing cell always escapes from its exosporium shell by popping through the cap, suggesting that the cap is designed to facilitate the emergence of the outgrowing cell.
Vibrotactile thresholds at the sole of the foot: Effect of vibration frequency and contact location (2011)

Studies of vibration perception in the glabrous skin of the human hand have identified four mechanoreceptor channels, with each channel showing characteristic variations in thresholds with variations in the frequency of vibration and the area of vibration excitation. To advance understanding of the channels mediating vibration perception on the sole of the foot, this study determined how thresholds depend on the frequency of vibration, the location on the foot (the big toe, the ball of the foot, and the heel), and the gap between a vibrating probe and a fixed surround. Thresholds at the three locations were obtained at the 12 preferred one-third octave centre frequencies from 20 to 250 Hz using a 6-mm diameter probe with both a 10-mm and a 20-mm diameter surround. With the 10-mm surround, the displacement thresholds at all three locations showed flat responses from 20 to 40 Hz. With both the 10-mm and the 20-mm surround, the displacement thresholds at the three locations showed "U-shaped" responses from 40 to 250 Hz. Relative to thresholds obtained with the 20-mm surround, thresholds obtained with the 10-mm surround were lower at the toe and the heel with 20- and 25-Hz vibration, but higher at the ball of the foot with 31.5- to 250-Hz vibration. It is concluded that absolute thresholds for the perception of vibration at the sole of the foot show important variations with location and with contact conditions and tend to be mediated by the NP I channel in the range from about 20 to 40 Hz and the P channel from about 40 to 250 Hz.
Talbot moire deflectrometry with direct spatial reconstruction of optical phase (1998)

Improvements in Talbot-moire deflectometry are presented. A Talbot-moire interferometer, using a laser diode as light source, a single grating and a CCD camera, placed in one of the Talbot planes, was set-up. The computer yields a moire pattern by mixing the acquired Talbot image of the first grating with the reference generated by computer or stored as an undisturbed external grating Talbot image. For moire pattern processing, direct spatial reconstruction of optical phase, in spatial and Fourier domains, implemented in a high-level image processing language (IDL<SUP>TM</SUP>), was employed. The theoretical limit of the relative error of Talbot-moire deflectometry was verified by measuring the focal length of a lenticular system with controlled-focal- length.
Determination of Research Octane Number using NIR Spectral Data (2001)

Ridge regression is compared with multiple linear regression (MLR) for determination of Research Octane Number (RON) when the baseline and signal-to-noise ratio are varied. MLR analysis of near-infrared (NIR) spectroscopic data usually encounters a collinearity problem, which adversely affects long-term prediction performance. The collinearity problem can be eliminated or greatly improved by using ridge regression, which is a biased estimation method. To evaluate the robustness of each calibration, the calibration models developed by both calibration methods were used to predict RONs of gasoline spectra in which the baseline and signalto-noise ratio were varied. The prediction results of a ridge calibration model showed more stable prediction performance as compared to that of MLR, especially when the spectral baselines were varied. . In conclusion, ridge regression is shown to be a viable method for calibration of RON with the NIR data when only a few wavelengths are available such as hand-carry device using a few diodes.
Development of parthenogenetic membranes in double‐yolked and injected chicken eggs (1975)

Development of parthenogenetic eggs is expressed in a Dark Cornish stock by either membrane or embryo formation. Double-yolked eggs have membranes developing in zero, one, or two yolks. The incidence of double-yolked eggs with two membranes was higher than expected when compared with the incidence of single-yolked eggs from the same hens. Membrane formation was increased over that of the controls by injecting saline, Ringer's solution, or ground crude membranes. Yolk and membranes filtered through a 3 mu filter or autoclaved did not increase membrane formation over that of the controls. Fertile membranes behaved like parthenogenetic membranes.
On the Total Energy Efficiency of Cell-Free Massive MIMO (2017)

We consider the cell-free massive multiple-input multiple-output (MIMO) downlink, where a very large number of distributed multiple-antenna access points (APs) serve many single-antenna users in the same time-frequency resource. A simple (distributed) conjugate beamforming scheme is applied at each AP via the use of local channel state information (CSI). This CSI is acquired through time-division duplex operation and the reception of uplink training signals transmitted by the users. We derive a closed-form expression for the spectral efficiency taking into account the effects of channel estimation errors and power control. This closed-form result enables us to analyze the effects of backhaul power consumption, the number of APs, and the number of antennas per AP on the total energy efficiency, as well as, to design an optimal power allocation algorithm. The optimal power allocation algorithm aims at maximizing the total energy efficiency, subject to a per-user spectral efficiency constraint and a per-AP power constraint. Compared with the equal power control, our proposed power allocation scheme can double the total energy efficiency. Furthermore, we propose AP selections schemes, in which each user chooses a subset of APs, to reduce the power consumption caused by the backhaul links. With our proposed AP selection schemes, the total energy efficiency increases significantly, especially for large numbers of APs. Moreover, under a requirement of good quality-of-service for all users, cell-free massive MIMO outperforms the colocated counterpart in terms of energy efficiency.
High-Avidity Monoclonal Antibodies against the Human Scavenger Class B Type I Receptor Efficiently Block Hepatitis C Virus Infection in the Presence of High-Density Lipoprotein (2007)

The human scavenger class B type 1 receptor (SR-B1/Cla1) was identified as a putative receptor for hepatitis C virus (HCV) because it binds to soluble recombinant HCV envelope glycoprotein E2 (sE2). High-density lipoprotein (HDL), a natural SR-B1 ligand, was shown to increase the in vitro infectivity of retroviral pseudoparticles bearing HCV envelope glycoproteins and of cell culture-derived HCV (HCVcc), suggesting that SR-B1 promotes viral entry in an HDL-dependent manner. To determine whether SR-B1 participates directly in HCV infection or facilitates HCV entry through lipoprotein uptake, we generated a panel of monoclonal antibodies (MAbs) against native human SR-B1. Two of them, 3D5 and C167, bound to conformation-dependent SR-B1 determinants and inhibited the interaction of sE2 with SR-B1. These antibodies efficiently blocked HCVcc infection of Huh-7.5 hepatoma cells in a dose-dependent manner. To examine the role of HDL in SR-B1-mediated HCVcc infection, we set up conditions for HCVcc production and infection in serum-free medium. HCVcc efficiently infected Huh-7.5 cells in the absence of serum lipoproteins, and addition of HDL led to a twofold increase in infectivity. However, the HDL-induced enhancement of infection had no impact on the neutralization potency of MAb C167, despite its ability to inhibit both HDL binding to cells and SR-B1-mediated lipid transfer. Of note, MAb C167 also potently blocked Huh-7.5 infection by an HCV strain recovered from HCVcc-infected chimpanzees. These results demonstrate that SR-B1 is essential for infection with HCV produced in vitro and in vivo and suggest the possible use of anti-SR-B1 antibodies as therapeutic agents.
A federated architecture approach for Internet of Things security (2014)

Internet of Things (IoT) refers to the capability to connect, communicate and remotely manage a large number of networked, automated devices via the Internet. IoT is becoming as part of daily life and aims to extend pervasive communication and networking anytime, anywhere with any device. In this context security requirements and architectures must be properly formulated, implemented in order to enforce the security policies during their life-cycle. This paper provides a survey and analysis of security in the area of IoT introducing an approach addressed to overcome the conventional security solutions and deploy a federated architecture for dynamic prevention, detection, diagnosis, isolation, and countermeasures against cyber attacks. Based on the analysis of the most common web services, the paper defines the security needs proposing a federated model to design an architecture for secure exchange of services in IoT paradigm.
A combined high-voltage, high-energy pulse generator (1994)

A novel high-voltage pulse generator, which can provide a rapid high-voltage, low-energy pulse followed by a high-energy capacitive discharge, is described. The generator simulates a high-voltage, high-energy pulsed power source without resorting to high-voltage, high-energy capacitors., The pulse-forming network is capable of generating a high-voltage pulse of up to 350 kV, and the capacitor bank can provide a high-current pulse of several tens of kilo-amperes.
High-level expression of adeno-associated virus (AAV) Rep78 or Rep68 protein is sufficient for infectious-particle formation by a rep-negative AAV mutant (1995)

Adeno-associated virus (AAV) codes for four closely related nonstructural proteins (Rep) required for AAV DNA replication and gene regulation. In vitro studies have revealed that either Rep78 or Rep68 alone is sufficient for AAV DNA replication. Rep52 and Rep40 are not required for DNA replication but have been reported to enhance the efficiency of accumulation of single-stranded progeny DNA. Previous studies on rep-expressing cell lines had indicated that only a subset of the four Rep proteins are required for the production of infectious AAV. We therefore set out to determine the minimal set of Rep proteins sufficient for the generation of infectious AAV. Transient cotransfections in HeLa cells of constructs for high-level expression of individual Rep proteins with a rep-negative AAV genome revealed that either Rep78 or Rep68 alone could complement for a full replication cycle yielding infectious virus. This result was confirmed by transfection studies in the cell line HeM2, which selectively expresses Rep78 at rather low levels under the control of the glucocorticoid-responsive mouse mammary tumor virus long terminal repeat (C. Hölscher, M. Hörer, J. A. Kleinschmidt, H. Zentgraf, A. Bürkle, and R. Heilbronn, J. Virol. 68:7169-7177, 1994). Increasing the level of Rep78 expression by transfection of a glucocorticoid receptor expression construct resulted in a higher level of DNA replication of a cotransfected rep-negative AAV genome and in the production of infectious rep-negative AAV particles. We further report on the generation of a new rep-expressing cell line, HeCM1, which was obtained by stable supertransfection of a construct for constitutive Rep40 expression into HeM1 cells (Hölscher et al., J. Virol. 68:7169-7177). Transfection of rather large amounts of rep-negative AAV DNA led to detectable virus production in HeCM1 cells even in the absence of the cotransfected glucocorticoid receptor expression construct, but higher yields were obtained after increasing the Rep78 level by coexpression of the glucocorticoid receptor. These data demonstrate that all Rep functions required for the productive replication of AAV in HeLa cells are contained within both Rep78 and Rep68.
A highly interdigitated GTO power switch with recessed gate structure (1984)

A highly interdigitated power GTO has been realized with a new technology based on reactive ion etching. The characteristic feature of the device are 50 µm wide vertically walled cathode fingers 30 µm apart from each other. The gate level is recessed 20 µm. The gate metalization is contacted directly on the recessed p-base having a doping concentration ≤2 ċ 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">16</sup> cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-3</sup> . With an ohmic load the turn off dI/dt is about 250 A/µsec cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> (related to the shorted anode area). Storage and fall time are well below 1 µsec at turn off gains close to one. The critical current crowding effect observed in conventional GTOs does not occur in the presented highly interdigitated structure.
Stable Decentralized Control of a Platoon of Vehicles With Heterogeneous Information Feedback (2013)

The problem of controlling a string of vehicles moving in one dimension is considered so that they all follow a lead vehicle with constant spacing between successive vehicles. Due to practical design and implementation, the negative effect of the tracking lag parameter is taken into account. A hierarchical platoon controller design framework is established comprising a feedback linearization controller at the first layer and a decentralized bidirectional control controller at the second layer. The stability criterion is examined by using a partial differential equation approximation in the limit of the number of vehicles subjected to unequal asymmetry in position and velocity feedback. For disturbance attenuation, string stability analysis is also examined. At the end of the paper, simulations are given to show the efficiency of the proposed results.
Comparative effectiveness review of treatment options for pituitary microadenomas in acromegaly (2012)

The choice of management strategy must be individualized for each patient with acromegaly. This CER analysis provides a comprehensive framework to inform clinical decisions among alternate management strategies in patients with GH-secreting pituitary microadenomas.
Analysis and comparison of image enhancement techniques for the prediction of lung cancer (2017)

Presently, lung cancer is identified in 2.6 million people and resulted in 1.8 million deaths. It is the most common type of cancer occurring in men and women. Smoking and air pollution are the main reason for lung cancer. So there is a need for identifying the cancerous cells present in the early stages within a shorter period and provides proper solution. Research work aiming at Image enhancement technique which will help in the detection of lung cancer in the earlier stages. A new technique is proposed to overcome the drawbacks for image enhancement using Gabor Filters, Discrete Wavelet Transform (DWT), and Auto Enhancement Algorithm (AEA). In this article, X-ray lung images are considered and processed using various techniques-Gabor filter, DWT and AEA. For real time analysis, obtained results are comparable with standard values. Hence, this new technique using Gabor filter for Image Enhancement using can be used for immediate detection of cancerous cells in patients.
The Early Mendicants (2020)

Abstract This chapter considers the early history of the women and men of the medieval Mendicant orders: the Franciscans, Dominicans, Carmelites, Augustinian Hermits, Servites, Sack, and Pied friars. It outlines recent revisionary approaches to continuity with previous religious orders and the multiplicity of forms ‘mendicancy’ might take. In doing so, it considers the essential features of what it meant to be a mendicant, male or female, paying particular attention to the changing significance of poverty and begging, the location of settlements, the slow emergence of a mendicant status, and the considerable role of the papacy. The value of non-normative textual and visual evidence is particularly emphasized, exposing the power of contingency and suggesting one of the creative ways in which research might begin to tackle the many questions that still deserve investigation.
UV-induced Hyperphosphorylation of Replication Protein A Depends on DNA Replication and Expression of ATM Protein (2001)

Exposure to DNA-damaging agents triggers signal transduction pathways that are thought to play a role in maintenance of genomic stability. A key protein in the cellular processes of nucleotide excision repair, DNA recombination, and DNA double-strand break repair is the single-stranded DNA binding protein, RPA. We showed previously that the p34 subunit of RPA becomes hyperphosphorylated as a delayed response (4-8 h) to UV radiation (10-30 J/m(2)). Here we show that UV-induced RPA-p34 hyperphosphorylation depends on expression of ATM, the product of the gene mutated in the human genetic disorder ataxia telangiectasia (A-T). UV-induced RPA-p34 hyperphosphorylation was not observed in A-T cells, but this response was restored by ATM expression. Furthermore, purified ATM kinase phosphorylates the p34 subunit of RPA complex in vitro at many of the same sites that are phosphorylated in vivo after UV radiation. Induction of this DNA damage response was also dependent on DNA replication; inhibition of DNA replication by aphidicolin prevented induction of RPA-p34 hyperphosphorylation by UV radiation. We postulate that this pathway is triggered by the accumulation of aberrant DNA replication intermediates, resulting from DNA replication fork blockage by UV photoproducts. Further, we suggest that RPA-p34 is hyperphosphorylated as a participant in the recombinational postreplication repair of these replication products. Successful resolution of these replication intermediates reduces the accumulation of chromosomal aberrations that would otherwise occur as a consequence of UV radiation.
Pretreatment of Lodgepole Pine Killed by Mountain Pine Beetle Using the Ethanol Organosolv Process:  Fractionation and Process Optimization (2007)

Lodgepole pine (Pinus contorta) killed by mountain pine beetle (Dendroctonus ponderosae) (MPB-LPP) was evaluated for bioconversion to ethanol using the ethanol organosolv process. The pretreatment was optimized using an experimental matrix designed with response surface methodology. It was found that MPB-LPP was easy to pretreat and delignify, but gave low yields of substrate and carbohydrate as a result of excessive hydrolysis and subsequent decomposition of cellulose and hemicellulose during the pretreatment. The center-point conditions (170 °C, 60 min, 1.1% H2SO4 and 65% ethanol) were close to the optimum for the recovery of glucose and ethanol organosolv lignin. At the center-point conditions, ∼75% of the cellulose present in the untreated wood was recovered in the substrate fraction, and approximately 79% of the lignin in the wood was recovered as ethanol organosolv lignin (EOL). The combined recovery of carbohydrate in the substrate and water-soluble fractions was ∼83% glucose, ∼46% mannose, ∼53% xylose, ∼78% galactose, and ∼55% arabinose. The lost carbohydrate was decomposed to furfural, hydroxymethylfurfural, and levulinic and formic acids. The substrate generated at center-point conditions from MPB-LPP was readily digestible. Cellulose-to-glucose conversion yields of ∼93% and ∼97% were achieved within 24 and 48 h, respectively, with 20 FPU of cellulase/g of cellulose.
Practical Approach for Determining Glomerular Filtration Rate by Single-Injection Inulin Clearance (1992)

We compared the glomerular filtration rate as measured by a single-injection inulin clearance with that measured by a standard isotope method with 99mTc-labeled diethylenetriaminopentaacetic acid in 21 subjects with glomerular filtration rates greater than 35 mL/min. After a bolus injection of 5 g of inulin, blood samples were taken 20, 45, 90, 120, 145, 180, and 240 min afterwards. Inulin was measured by optimized chemical or enzymatic methods of high analytical sensitivity to determine inulin at low concentrations. We used the one-compartment model and inulin concentrations measured at two sampling times to calculate the glomerular filtration rate from the data of the disappearance curve of inulin. Inulin concentrations at 20 and 240 min after injection of the inulin bolus were suited to estimate glomerular filtration rate by this procedure, resulting in values (y) comparable with those obtained by isotope technique (x). The relationship to the isotope technique was characterized by the equation y = +4.80 mL/min + 0.92x (r = 0.97). The single-injection inulin clearance determination can detect a decrease of glomerular filtration rate at the beginning of kidney damage, given that our study included subjects with glomerular filtration rates greater than 35 mL/min. We conclude that the glomerular filtration rate can be determined by analyzing only two blood samples after a bolus injection of inulin.
Privacy-Preserving Access Control in Cloud Federations (2017)

A Cloud federation is a collaboration of organizations sharing data hosted on their private cloud infrastructures in order to exploit a common business opportunity. However, the adoption of cloud federations is hindered by member organizations' concerns on sharing their data with potentially competing organizations. For cloud federations to be viable, federated organizations' privacy concerns should be alleviated by providing mechanisms that allow organizations to control which users from other federated organizations can access which data. We propose the architecture of a novel identity and access management system part of FaaS, a cloud federation service developed by the H2020 SUNFISH project. Our system allows federated organizations to enforce attribute-based access control policies on their data in a privacy-preserving fashion. Users are granted access to federated data when their identity attributes match the policies, but without revealing their attributes in clear. The architecture relies on two novel technologies, blockchain and Intel SGX hardware platform to guarantee integrity of the policy evaluation process.
Blind image quality assessment on real distorted images using deep belief nets (2014)

We present a novel natural-scene-statistics-based blind image quality assessment model that is created by training a deep belief net to discover good feature representations that are used to learn a regressor for quality prediction. The proposed deep model has an unsupervised pre-training stage followed by a supervised fine-tuning stage, enabling it to generalize over different distortion types, mixtures, and severities. We evaluated our new model on a recently created database of images afflicted by real distortions, and show that it outperforms current state-of-the-art blind image quality prediction models.