gebhart/patrepeval huggingface dataset:
Signal Processing, Glycosylation, and Secretion of Mutant Hemagglutinins of a Human Influenza Virus by <i>Saccharomyces cerevisiae</i> (1987)

We investigated the nature of signal recognition, transport, and secretion of mutant hemagglutinins (HAs) of a human influenza virus by the yeast Saccharomyces cerevisiae. The cDNA sequences encoding variant forms of influenza HA were expressed in S. cerevisiae. The HA polypeptides (HA500 and HA325) that were synthesized with their N-terminal signal peptides were correctly targeted to the membrane compartment where they were glycosylated. In contrast, the HA polypeptides (HA484 and HA308) lacking the signal peptide were expressed in the cytoplasm and did not undergo any glycosidic modification, demonstrating the importance of the heterologous signal sequence in the early steps of translocation in S. cerevisiae. The analysis of the N-terminal amino acid sequence of HA500 and HA325 polypeptides demonstrated the correct cleavage of the signal peptide, indicating the structural compatibility of a heterologous signal peptide for efficient recognition and processing by the yeast translocation machinery. The membrane-sequestered and glycosylated HA polypeptides were relatively stable in S. cerevisiae compared with the signal-minus, nonglycosylated HA molecules. Although both the anchor-minus HA (HA500) and HA1 (HA325) polypeptides were targeted efficiently to the membrane, their glycosylation and transport patterns were shown to be different. During pulse-chase, the HA500 remained cell-associated with no detectable secretion into the extracellular medium, whereas the HA325 secreted into the medium. Furthermore, only the cell-associated and secreted forms of HA325 and not HA500 appeared to have undergone hyperglycosylation with the extensive addition of high-molecular-weight outer-chain mannans. Possible reasons for the observed phenotypic behavior of these two mutant HAs are discussed.
Passive electrophoresis in microchannels using liquid junction potentials (2002)

The formation of the liquid junction potential (LJP) is a well-studied phenomenon that occurs in the presence of ionic concentration gradients. Although the LJP has been well characterized, its impact has generally been overlooked in microfluidic applications. The characteristics of flow in microfluidic channels cause this phenomenon to be particularly important, both as a source of deviation from anticipated results and as a tool capable of being harnessed to perform useful tasks. It is demonstrated that LJPs formed in microchannels can induce appreciable electrophoretic transport of charged species without the use of electrodes or an external power supply. This process is demonstrated in an H-filter (an H-shaped microfluidic channel used to bring two fluids into contact allowing extraction of diffusing species from one stream to another) by generating junction potentials between two flowing streams containing different concentrations of strong electrolytes and observing the mass transport of the charged dye fluorescein between those streams. It is shown that the LJP can be controlled to either accelerate or decelerate mass transport across a fluid interface in the absence of an interposed membrane. A preliminary mathematical description of the phenomena is offered to support the hypothesis that the observed mass transport is a result of the LJP. Possible practical microfluidic applications of electrophoretic transport without electrodes are discussed.
Evaluation of Intra-Subband Power Allocation for a Downlink Non-Orthogonal Multiple Access (NOMA) System (2016)

Non-Orthogonal Multiple Access (NOMA) schemes superpose multiple users in the power domain. It is commonly assumed that the achieved throughput in a downlink NOMA system increases with the channel gain difference between paired users. This paper investigates the accuracy of this claim when different intra-subband power allocation techniques are studied. Moreover, the Proportional Fairness (PF) scheduler is used as a resource allocation mechanism to highlight the importance of the choice of user pairing and of the intra-subband power allocation scheme. Results show that the achieved throughput does not always evolve in the same direction as the channel gain difference between paired users, owing to the choice of the intra-subband power allocation technique. In addition, it was found that the PF scheduler is not fully adapted to NOMA.
The influence of thermal, hydrologic, and snow deformation mechanisms on snow water equivalent pressure sensor accuracy (2002)

Abstract A 5 year field study was conducted to determine the mechanisms that cause snow water equivalent (SWE) pressure sensor measurement errors. The objective is to establish design and installation criteria to develop an accurate electronic SWE pressure sensor that minimizes errors. We monitored a 3 m snow pillow and installed three prototype electronic SWE sensors of our own design to examine how SWE errors occur. We also measured the heat flux through the prototype sensors and the soil, snow temperature, soil moisture content, and soil thermal conductivity. The SWEs of snow cores were used to assess the accuracy of the snow pillow and prototype sensors. Experimental results indicate that SWE measurement errors occur only when the snow–SWE sensor and/or the snow–soil interfaces are at the melting temperature. The magnitude of SWE errors is related to the diameter of the sensor and the difference in heat flux through the sensor and the surrounding soil. SWE over‐measurement errors occur when the heat flux through the sensor is less than through the surrounding soil, producing a snowmelt rate on the sensor that is less than on the adjacent soil. SWE under‐measurement errors occur when the heat flux through the sensor is greater than through the surrounding soil. The most severe SWE measurement errors occur during the transition from winter to spring, when the snow cover first reaches an isothermal condition causing a maximum difference in snowmelt rate between an SWE sensor and the surrounding soil. SWE measurement errors are reduced by increasing the SWE sensor diameter, matching the thermal properties of the soil and SWE sensor, allowing water to flow through the sensor, and using a surface cover to diffuse heat into the adjacent soil. SWE measurement errors relax through snow creep mechanisms that redistribute the snow load equally between the sensor and surrounding soil. Published in 2002 by John Wiley & Sons Ltd.
Collapsin Response Mediator Protein-1 and the Invasion and Metastasis of Cancer Cells (2001)

CRMP-1 appears to be involved in cancer invasion and metastasis and may be an invasion-suppressor gene.
High-Throughput Signal Component Separator for Asymmetric Multi-Level Outphasing Power Amplifiers (2013)

This paper presents an energy-efficient high-throughput and high-precision signal component separator (SCS) chip design for the asymmetric-multilevel-outphasing (AMO) power amplifier. It uses a fixed-point piece-wise linear functional approximation developed to improve the hardware efficiency of the outphasing signal processing functions. The chip is fabricated in 45 nm SOI CMOS process and the SCS consumes an active area of 1.5 mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> . The new algorithm enables the SCS to run at a throughput of 3.4 GSamples/s producing the phases with 12-bit accuracy. Compared to traditional low-throughput AMO SCS implementations, at 0.8 GSamples/s this design improves the area efficiency by 25× and the energy-efficiency by 2×. This fastest high-precision SCS to date enables a new class of high-throughput mm-wave and base station transmitters that can operate at high area, energy and spectral efficiency.
Comparison of cyclic and linear analogs of vasoactive intestinal peptide. (1996)

A series of i-->i + 4 side-chain to side-chain lactam analogs of vasoactive intestinal peptide has been prepared in order to study the effect of cyclization on biological activity. In vitro, on guinea pig tracheal smooth muscle and on human bronchial tissue, approximately half of the cyclic analogs showed increased potency and half were decreased over the linear analogs. Several cyclic compounds were between 10- and 20-fold more potent and one was 290-fold more potent than the linear species. In vivo, in guinea pigs, the cyclic compounds showed increased potency by up to 70-fold and significantly enhanced duration of action as compared to linear compounds.
Determination of structural elements related to the biological activities of a potent decapeptide agonist of human C5a anaphylatoxin (1999)

The structural features related to the biologic activities of a potent, response-selective decapeptide agonist of human C5a, YSFKPMPLaR (C5a65-74, Y65, F67, P69, P71, D-Ala73), were identified by NMR analysis in H2O, DMSO and TFE. This investigation showed that the KPM residues in H2O and the SFKPM residues in DMSO exhibited an extended backbone conformation, whereas a twisted conformation was found in this region in TFE. In H2O, the C-terminal region (PLaR) adopted a distorted type II beta-turn or a type II/V beta-turn. In the type IIN beta-turn, Leu72 exhibited a conformation typical of a type II beta-turn, whereas D-Ala73 exhibited a conformation characteristic of a type V beta-turn. Furthermore, a gamma-turn involving residues LaR overlapped with the type II/V beta-turn. In DMSO, the C-terminal region had the analogous turn-like motif (type II/V beta-turn overlapping with gamma-turn) found in H2O. In TFE, no beta-turn motifs were formed by the PLaR residues. These turn-like motifs in the C-terminal region of the peptide in both H2O and DMSO were in agreement with the biologically important conformations predicted earlier by a structure-function analysis of a related panel of decapeptide analogs. The motifs determined by the NMR analysis of YSFKPMPLaR in H2O and DMSO may represent structural elements important for C5a agonist activity and thus can be used to design the next generation of C5a agonist, partial agonist and antagonist analogs.
Novel Technique for Enhancing the Strength of Friction Stir Spot Welds through Dynamic Welding Parameters (2021)

Presently, friction stir spot welding (FSSW) has become a common alternative for spot welding technologies. Over the years, researchers have implemented various methods for enhancing weld strength. However, the literature shows that the previously reported approaches have used static (constant) welding parameters set at the beginning of the welding stroke (i.e., the FSSW parameters were kept constant during the welding stroke). In contrast, in this study, an innovative technique is proposed for enhancing the weld strength for Al 1050 material by adjusting the FSSW process parameters during the welding stroke. Two FSSW parameters, namely, feed rate and spindle speed (dynamic parameters), are used in this study with a stepwise variation function and are changed during the welding stroke. The results of this study show that the weld tensile strength is enhanced by 12–21% when using the proposed novel dynamic welding parameter technique. This is a significant increase in the weld strength compared to when static welding parameters are employed during the welding stroke.
Transformations and resynthesis for testability of RT-level control-data path specifications (1993)

This paper introduces a technique to transform a given register-transfer level (RT-level) design, consisting of control logic and data path, into a functionally equivalent, minimized design which is 100% testable under full-scan at the gate level. The proposed RT-level optimization technique uses the RT-level structure and exploits the interaction between the control and the data path. Our approach maintains the RT-level design hierarchy while performing RT-level transformations of initially specified data path, followed by resynthesis of control using don't cares extracted from the data path. Experiments with several RTL benchmarks demonstrate the effectiveness of the technique in generating fully testable designs. In addition, comparison with logic-level techniques show the advantages of the proposed technique as an optimizing tool to produce circuits with reduced area and delay.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
SSIM-Inspired Perceptual Video Coding for HEVC (2012)

Recent advances in video capturing and display technologies, along with the exponentially increasing demand of video services, challenge the video coding research community to design new algorithms able to significantly improve the compression performance of the current H.264/AVC standard. This target is currently gaining evidence with the standardization activities in the High Efficiency Video Coding (HEVC) project. The distortion models used in HEVC are mean squared error (MSE) and sum of absolute difference (SAD). However, they are widely criticized for not correlating well with perceptual image quality. The structural similarity (SSIM) index has been found to be a good indicator of perceived image quality. Meanwhile, it is computationally simple compared with other state-of-the-art perceptual quality measures and has a number of desirable mathematical properties for optimization tasks. We propose a perceptual video coding method to improve upon the current HEVC based on an SSIM-inspired divisive normalization scheme as an attempt to transform the DCT domain frame prediction residuals to a perceptually uniform space before encoding. Based on the residual divisive normalization process, we define a distortion model for mode selection and show that such a divisive normalization strategy largely simplifies the subsequent perceptual rate-distortion optimization procedure. We further adjust the divisive normalization factors based on local content of the video frame. Experiments show that the proposed scheme can achieve significant gain in terms of rate-SSIM performance when compared with HEVC.
Field Performance of Photovoltaic Solar Water Heating Systems (1997)

Energy consumed for water heating accounts for approximately 17.9 EJ of the energy consumed by residential and commercial buildings. Although there are over 90 million water heaters currently in use within the United States (Zogg and Barbour, 1996), durability and installation issues as well as initial cost have limited the sales of solar water heaters to less than 1 million units. Durability issues have included freeze and fluid leakage problems, failure of pumps and their associated controllers, the loss of heat transfer fluids under stagnation conditions, and heat exchanger fouling. The installation of solar water heating systems has often proved difficult, requiring roof penetrations for the piping that transports fluid to and from the solar collectors. Fanney and Dougherty have recently proposed and patented a solar water heating system that eliminates the durability and installation problems associated with current solar water heating systems. The system employs photovoltaic modules to generate electrical energy which is dissipated in multiple electric heating elements. A microprocessor controller is used to match the electrical resistance of the load to the operating characteristics of the photovoltaic modules. Although currently more expensive than existing solar hot water systems, photovoltaic solar water heaters offer the promise of being less expensive than solar thermal systems within the next decade. To date, photovoltaic solar water heating systems have been installed at the National Institute of Standards and Technology in Gaithersburg, MD and the Florida Solar Energy Center in Cocoa, FL. This paper will review the technology employed, describe the two photovoltaic solar water heating systems, and present measured performance data.
A site-specific, multiplexed kinase activity assay using stable-isotope dilution and high-resolution mass spectrometry (2009)

Most kinases are capable of recognizing and phosphorylating peptides containing short, linear sequence motifs. To measure the activation state of many kinases from the same cell lysate, we created a multiplexed, mass-spectrometry-based in vitro kinase assay. Ninety chemically synthesized peptides derived from well-characterized peptide substrates and in vivo phosphorylation sites with either known or previously unidentified upstream kinases were reacted individually in a plate format with crude cell lysates and ATP. Phosphorylation rates were directly measured based on the addition of 90 same-sequence, site-specific phosphopeptides enriched in stable isotopes to act as ideal quantitative internal standards for analysis by liquid chromatography coupled to tandem mass spectrometry. This approach concurrently measured up to 90 site-specific peptide phosphorylation rates, reporting a diagnostic fingerprint for activated kinase pathways. We applied this unique kinome-activity profiling strategy in a variety of cellular settings, including mitogen stimulation, cell cycle, pharmacological inhibition of pathways, and to a panel of breast cancer cell lines. Finally, we identified the source of activity for a peptide (derived from a PI3K regulatory subunit) from our library. This peptide substrate demonstrated mitotic and tyrosine-specific phosphorylation, which was confirmed to be a novel Src family kinase site in vivo.
Synthesis and Electronic Properties of Regioisomerically Pure Oxochlorins (2002)

We describe a two-step conversion of C-alkylated zinc chlorins to zinc oxochlorins wherein the keto group is located in the reduced ring (17-position) of the macrocycle. The transformation proceeds by hydroxylation upon exposure to alumina followed by dehydrogenation with DDQ. The reactions are compatible with ethyne, iodo, ester, trimethylsilyl, and pentafluorophenyl groups. A route to a spirohexyl-substituted chlorin/oxochlorin has also been developed. Representative chlorins and oxochlorins were characterized by static and time-resolved absorption spectroscopy and fluorescence spectroscopy, resonance Raman spectroscopy, and electrochemistry. The fluorescence quantum yields of the zinc oxochlorins (Phi(f) = 0.030-0.047) or free base (Fb) oxochlorins (Phi(f) = 0.13-0.16) are comparable to those of zinc tetraphenylporphyrin (ZnTPP) or free base tetraphenylporphyrin (FbTPP), respectively. The excited-state lifetimes of the zinc oxochlorins (tau = 0.5-0.7 ns) are on average 4-fold lower than that of ZnTPP, and the lifetimes of the Fb oxochlorins (tau = 7.4-8.9 ns) are approximately 40% shorter than that of FbTPP. Time-resolved absorption spectroscopy of a zinc oxochlorin indicates the yield of intersystem crossing is >70%. Resonance Raman spectroscopy of copper oxochlorins show strong resonance enhancement of the keto group upon Soret excitation but not with Q(y)()-band excitation, which is attributed to the location of the keto group in the reduced ring (rather than in the isocyclic ring as occurs in chlorophylls). The one-electron oxidation potential of the zinc oxochlorins is shifted to more positive potentials by approximately 240 mV compared with that of the zinc chlorin. Collectively, the fluorescence yields, excited-state lifetimes, oxidation potentials, and various spectral characteristics of the chlorin and oxochlorin building blocks provide the foundation for studies of photochemical processes in larger architectures based on these chromophores.
Cooperative Localization in Wireless Networks (2009)

Location-aware technologies will revolutionize many aspects of commercial, public service, and military sectors, and are expected to spawn numerous unforeseen applications. A new era of highly accurate ubiquitous location-awareness is on the horizon, enabled by a paradigm of cooperation between nodes. In this paper, we give an overview of cooperative localization approaches and apply them to ultrawide bandwidth (UWB) wireless networks. UWB transmission technology is particularly attractive for short- to medium-range localization, especially in GPS-denied environments: wide transmission bandwidths enable robust communication in dense multipath scenarios, and the ability to resolve subnanosecond delays results in centimeter-level distance resolution. We will describe several cooperative localization algorithms and quantify their performance, based on realistic UWB ranging models developed through an extensive measurement campaign using FCC-compliant UWB radios. We will also present a powerful localization algorithm by mapping a graphical model for statistical inference onto the network topology, which results in a net-factor graph, and by developing a suitable net-message passing schedule. The resulting algorithm (SPAWN) is fully distributed, can cope with a wide variety of scenarios, and requires little communication overhead to achieve accurate and robust localization.
Loop Plant Electronics: Voice Frequency Electronics for Loop Applications (1978)

Over the past 100 years the telephone loop connecting central office switching equipment and the telephone customer's premises has evolved from aerial open wire to buried plastic-insulated twisted pairs. In the last 20 years the use of electronic circuits for range extension has become popular. These circuits allow use of higher resistance loops (finer gauge wires) than central offices are normally designed to accept. This paper reviews some of this history, describes rural and suburban systems of voice frequency range extension, presents requirements and circuit design considerations, and compares several electronic range extender circuits currently used to implement these systems.
Adaptive Access Control Scheme Utilizing Context Awareness in Pervasive Computing Environments (2008)

In pervasive computing environments, where various types of information are publicly owned, and multiple users access the networks via various networked devices anytime and anywhere, access control that grants permission to an authorized user is definitely needed for secure information access. Context awareness refers to the idea that computers can both sense and react based on various context in their environments. In many access control schemes, recently, context awareness has been utilized to guarantee dynamic access control according to current context and various access control schemes utilizing context awareness have been proposed. However, previous studies have difficulty describing conditions for assigning roles and modifying permissions. They also simply consider assigning roles or modifying permissions, rather than providing detailed access control algorithms such as role delegation or role revocation. In this paper, we propose an adaptive access control scheme utilizing context awareness in pervasive computing environments. We design an adaptive access control model based on traditional RBAC(Role-Based Access Control) model, and present an adaptive access control scheme to guarantee dynamic user and permission assignment according to changes of context. In this scheme, we define context requirements in each table, enabling a more detailed description. We also guarantee dynamic access control via various access control algorithms.