gebhart/patrepeval huggingface dataset:
Toward trustworthy mobile sensing (2010)

Commodity mobile devices have been utilized as sensor nodes in a variety of domains, including citizen journalism, mobile social services, and domestic eldercare. In each of these domains, data integrity and device-owners' privacy are first-class concerns, but current approaches to secure sensing fail to balance these properties. External signing infrastructure cannot attest to the values generated by a device's sensing hardware, while trusted sensing hardware does not allow users to securely reduce the fidelity of readings in order to preserve their privacy. In this paper we examine the challenges posed by the potentially conflicting goals of data integrity and user privacy and propose a trustworthy mobile sensing platform which leverages inexpensive commodity Trusted Platform Module (TPM) hardware.
Primase Activity of Human DNA Polymerase α-Primase (1998)

DNA polymerase alpha-primase consists of four subunits, p180, p68, p58, and p48, and comprises two essential enzymatic functions. To study the primase activity of the complex, we expressed cDNAs encoding for the human p58 and p48 subunits either as single proteins or together using Escherichia coli expression vectors. Co-expression of both primase subunits allowed the purification of a heterodimer in high yields that revealed stable primase activity. Purified recombinant p48 subunit showed enzyme activity, whereas purified p58 did not. In contrast to the heterodimer, the primase activity of p48 was unstable. The activity of p48 could be stabilized by the addition of the divalent cations Mg2+ and Mn2+ but not Zn2+. On a poly(dC) template the primase activity was hardly influenced by the monovalent cation potassium. However, by using poly(dT) as a template the recombinant p48 activity was sensitive to salt, whereas recombinant p58-p48 and the bovine DNA polymerase alpha-primase purified from thymus were less sensitive to the addition of monovalent cations. A complex of bacterially expressed primase and baculovirus-expressed p180 and p68 was assembled in vitro and shown to support replication of simian virus 40 DNA in a cell-free system.
ChemInform Abstract: MEMORY EFFECT AND 1,3‐DIAZEPINE RING CLOSURE IN ARYLNITRENIUM IONS (1983)

Abstract Die in situ aus N‐Hydroxy‐amidinen und TosCl/Et3N dargestellten Amidine (I) unterliegen a) der aromatischen Umlagerung zu den Stellungsisomeren (II)‐(V), b) einem 5‐und c) einem 7‐Ringschluß zu den Benzimidazolen (VI) und (VII) bzw. den Diazepinen (VIII).
The role of B-type esterases in conferring insecticide resistance in the tobacco whitefly,Bemisia tabaci (Genn) (2000)

Separation of non-specific esterases on electrophoretic gels has played a key role in distinguishing between races or biotypes of the tobacco whitefly, Bemisia tabaci. One intensively staining esterase in particular (termed E0.14) has assumed significance as a diagnostic of B-type whiteflies (aka Bemisia argentifolii), despite any knowledge of its biological function. In this study, a whitefly strain (B-Null) homozygous for a null allele at the E0.14 locus that had been isolated from a B-type population was used to demonstrate a significant role for E0.14 in resistance of B-type populations to pyrethroids but not to organophosphates (OPs). Bioassays with pyrethroids, following pre-treatment with sub-lethal doses of the OP profenofos (to inhibit esterase activity), coupled with metabolism studies with radiolabelled permethrin, supported the conclusion that pyrethroid resistance in a range of B-type strains expressing E0.14 was primarily due to increased ester hydrolysis. In the same strains, OP resistance appeared to be predominantly conferred by a modification to the target-site enzyme acetylcholinesterase. © 2000 Society of Chemical Industry
Coexpression of colanic acid and serotype-specific capsular polysaccharides in Escherichia coli strains with group II K antigens (1993)

In Escherichia coli K-12, the rcsA and rcsB gene products are positive regulators in expression of the slime polysaccharide colanic acid. We have previously demonstrated the presence of rcsA sequences in E. coli K1 and K5, strains with group II capsular K antigens, and shown that introduction of multicopy rcsA into these strains results in the expression of colanic acid. We report here the presence of rcsB sequences in E. coli K1 and K5 and demonstrate that RcsB also plays a role in the biosynthesis of colanic acid in strains with group II K antigens. In E. coli K1 and K5 grown at 37 degrees C, multicopy rcsB and the resulting induction of colanic acid synthesis had no significant effect on synthesis of the group II K antigens. K-antigen-specific sugar transferase activities were not significantly different in the presence or absence of multicopy rcsB, and introduction of a cps mutation to eliminate colanic acid biosynthesis in a K1-derivative strain did not influence the activity of the polysialyltransferase enzyme responsible for synthesis of the K1 polymer. Furthermore, immunoelectron microscopy showed no detectable difference in the size or distribution of the group II K-antigen capsular layer in cells which produced colanic acid. Colanic acid expression therefore does not appear to significantly affect synthesis of the group II K-antigen capsule and, unlike for group I K antigens, expression of group II K antigens is not positively regulated by the rcs system.
Isolation and characterization of<i>Streptococcus</i>sp. from diseased flounder (<i>Paralichthys olivaceus</i>) in Jeju Island (2006)

Streptococcus sp. is gram-positive coccus that causes streptococcal infections in fish due to intensification of aquaculture and caused significant economic losses in fish farm industry. A streptococcal infection occurred from cultured diseased olive flounder (Paralichthys olivaceus) in May, 2005 at a fish farm in Jeju Island, Korea. The diseased flounder exhibited bilateral exophthalmic eyes and rotten gills; water temperature was 16-18 degrees C when samples were collected. Of the 22 fish samples collected, 3 samples were identified as Lactococcus garvieae and 18 samples were identified as Streptococcus parauberis by culture-based, biochemical test. Serological methods such as slide agglutination, hemolysis and antimicrobial susceptibility test were also used as well as multiplex PCR-based method to simultaneously detect and confirm the pathogens involved in the infection. S. parauberis and L. garvieae have a target region of 700 and 1100 bp., respectively. One fish sample was not identified because of the difference in the different biochemical and serological tests and was negative in PCR assay. In the present study, it showed that S. parauberis was the dominant species that caused streptococcosis in the cultured diseased flounder.
Ionic Strength- and Temperature-Induced K <sub>Ca</sub> Shifts in the Uncoating Reaction of Rotavirus Strains RF and SA11: Correlation with Membrane Permeabilization (2002)

The hydrodynamic diameters of native rotavirus particles, bovine RF and simian SA11 strains, were determined by quasielastic light scattering. By using this method and agarose gel electrophoresis, the Ca(2+) dissociation constant, K(Ca), governing the transition from triple-layer particles (TLPs) to double-layer particles (DLPs), was shown to increase, at constant pH, as the temperature and/or the ionic strength of the incubation medium increased. We report the novel observation that, under physiological conditions, K(Ca) values for both RF and SA11 rotaviruses were well above the intracytoplasmic Ca(2+) concentrations of various cells, which may explain why TLP uncoating takes place within vesicles (possibly endosomes) during the entry process. A correlation between TLP uncoating and cell membrane permeabilization was found, as shown by the release of carboxyfluorescein (CF) from CF-loaded intestinal brush-border membrane vesicles. Conditions stabilizing the virion in the TLP form inhibited CF release, whereas conditions favoring the TLP-to-DLP transformation activated this process. We conclude that membrane permeabilization must be preceded by the loss of the outer-capsid proteins from trypsinized TLP and that physiological ionic strength is required for permeabilization to take place. Finally, the paper develops an alternative explanation for the mechanism of rotavirus entry, compatible with the Ca(2+)-dependent endocytic pathway. We propose that there must be an iterative process involving tight coupling in time between the lowering of endosomal Ca(2+) concentration, virion decapsidation, and membrane permeabilization, which would cause the transcriptionally active DLPs to enter the cytoplasm of cells.
Building Modern Clouds: Using Docker, Kubernetes & Google Cloud Platform (2019)

To develop and build a modern cloud infrastructure or DevOps implementation than both Docker and Kubernetes have revolutionized the era of software development and operations. Although both are different, they unify the process of development and integration, it is now possible to build any architecture by using these technologies. Docker is used to build, ship and run any application anywhere. Docker allows the use of the same available resources. These containers can be used to make deployments much faster. Containers use less space, are reliable and are very fast. Docker Swarm helps to manage the docker container. Kubernetes is an automated container management, deployment and scaling platform. Using Google Cloud Platform to deploy containers on Kubernetes Engine enabling rapid application development and management. Kubernetes provides key features like deployment, easy ways to scale, and monitoring.
Computational Framework for Simulating Fluorescence Microscope Images With Cell Populations (2007)

Fluorescence microscopy combined with digital imaging constructs a basic platform for numerous biomedical studies in the field of cellular imaging. As the studies relying on analysis of digital images have become popular, the validation of image processing methods used in automated image cytometry has become an important topic. Especially, the need for efficient validation has arisen from emerging high-throughput microscopy systems where manual validation is impractical. We present a simulation platform for generating synthetic images of fluorescence-stained cell populations with realistic properties. Moreover, we show that the synthetic images enable the validation of analysis methods for automated image cytometry and comparison of their performance. Finally, we suggest additional usage scenarios for the simulator. The presented simulation framework, with several user-controllable parameters, forms a versatile tool for many kinds of validation tasks, and is freely available at http://www.cs.tut.fi/sgn/csb/simcep.
Further evidence for lymphokine overproduction in severe aplastic anemia [see comments] (1988)

Interferon-gamma (IFN-gamma) and tumor necrosis factor (TNF) are lymphokines with a potent hematopoietic progenitor cell suppressive capacity. In untreated and immunosuppressed patients with severe aplastic anemia (SAA) and in control individuals we measured (a) serum levels of IFN-gamma and TNF and its production by peripheral blood mononuclear cells (PBMNC); (b) serum levels of neopterin, a product that reflects endogenous IFN production; (c) resting and activated lymphocyte subpopulations; and (d) serum levels of soluble interleukin- 2 receptor (IL-2R). Serum levels of IFN and TNF did not differ significantly in untreated and treated SAA patients and control individuals. Spontaneous and phytohemagglutinin-induced production of IFN and TNF by PBMNC, however, were highly increased in both untreated and treated SAA patients. Increased and decreased neopterin serum levels in untreated and treated SAA patients, respectively, suggest modulation of endogenous lymphokine release subsequent to immunosuppression. HLA-DR+ antigen was mainly expressed by CD8 T cells. Circulating numbers of activated (CD4 and CD8) T cells and serum levels of IL-2R were not increased in both untreated and treated SAA patients. The proportion of HLA-DR+ T cells in the PBMNC of untreated SAA patients correlated with the extent of lectin-induced IFN production. Although we were unable to confirm previous reports in SAA on (a) detectable IFN in blood and bone marrow serum, (b) improvement of stem cell growth upon neutralization of endogenous IFN, (c) absolutely increased numbers of circulating activated T cells, and (d) normalization of these abnormalities subsequent to successful immunosuppression, our data clearly support previous reports on abnormal lymphokine production in severe aplastic anemia. Our failure to relate this phenomenon to the severity of disease states, however, further raises doubts on the pathogenetic significance of lymphokine overproduction in SAA.
Isolation and Characterization of Three <i>Streptococcus pneumoniae</i> Transformation-Specific Loci by Use of a <i>lacZ</i> Reporter Insertion Vector (1998)

Although more than a dozen new proteins are produced when Streptococcus pneumoniae cells become competent for genetic transformation, only a few of the corresponding genes have been identified to date. To find genes responsible for the production of competence-specific proteins, a random lacZ transcriptional fusion library was constructed in S. pneumoniae by using the insertional lacZ reporter vector pEVP3. Screening the library for clones with competence-specific beta-galactosidase (beta-Gal) production yielded three insertion mutants with induced beta-Gal levels of about 4, 10, and 40 Miller units. In all three clones, activation of the lacZ reporter correlated with competence and depended on competence-stimulating peptide. Chromosomal loci adjacent to the integrated vector were subcloned from the insertion mutants, and their nucleotide sequences were determined. Genes at two of the loci exhibited strong similarity to parts of Bacillus subtilis com operons. One locus contained open reading frames (ORFs) homologous to the comEA and comEC genes in B. subtilis but lacked a comEB homolog. A second locus contained four ORFs with homology to the B. subtilis comG gene ORFs 1 to 4, but comG gene ORFs 5 to 7 were replaced in S. pneumoniae with an ORF encoding a protein homologous to transport ATP-binding proteins. Genes at all three loci were confirmed to be required for transformation by mutagenesis using pEVP3 for insertion duplications or an erm cassette for gene disruptions.
Radical Reactions with Alkyl and Fluoroalkyl (Fluorous) Tin Hydride Reagents in Supercritical CO<sub>2</sub> (1997)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTRadical Reactions with Alkyl and Fluoroalkyl (Fluorous) Tin Hydride Reagents in Supercritical CO2Sabine Hadida, Michael S. Super, Eric J. Beckman, and Dennis P. CurranView Author Information Department of Chemistry, University of Pittsburgh Pittsburgh, Pennsylvania 15260 Department of Chemical Engineering, University of Pittsburgh Pittsburgh, Pennsylvania 15261 Cite this: J. Am. Chem. Soc. 1997, 119, 31, 7406–7407Publication Date (Web):August 6, 1997Publication History Received8 April 1997Published online6 August 1997Published inissue 1 August 1997https://pubs.acs.org/doi/10.1021/ja971120ihttps://doi.org/10.1021/ja971120irapid-communicationACS PublicationsCopyright © 1997 American Chemical SocietyRequest reuse permissionsArticle Views734Altmetric-Citations79LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Anions,Chemical reactions,Organic reactions,Reagents,Solvents Get e-Alerts
A BASIC computer program for calculating indices of genetic distance and similarity (1979)

A computer program in the BASIC programming language is presented and described for the calculation of Nei's and Rogers' indices of genetic distance (D), identity (I), similarity (SR), and difference (DR) between many populations for use in microcomputer systems. Average homozygosity (Jx) is also displayed. Limitations on the number of alleles used and the number of populations compared are determined only by the memory size of the computer system employed and the physical limitations of printout devices.
A Functional Genomics Approach Reveals CHE as a Component of the <i>Arabidopsis</i> Circadian Clock (2009)

Transcriptional feedback loops constitute the molecular circuitry of the plant circadian clock. In Arabidopsis, a core loop is established between CCA1 and TOC1. Although CCA1 directly represses TOC1, the TOC1 protein has no DNA binding domains, which suggests that it cannot directly regulate CCA1. We established a functional genomic strategy that led to the identification of CHE, a TCP transcription factor that binds specifically to the CCA1 promoter. CHE is a clock component partially redundant with LHY in the repression of CCA1. The expression of CHE is regulated by CCA1, thus adding a CCA1/CHE feedback loop to the Arabidopsis circadian network. Because CHE and TOC1 interact, and CHE binds to the CCA1 promoter, a molecular linkage between TOC1 and CCA1 gene regulation is established.
A 128Mb ChainFeRAM<sup>TM</sup> and system designs for HDD application and enhanced HDD performance (2009)

This paper demonstrates the hard disk drive (HDD) performance improvement by nonvolatile FeRAM cache. First, the 128 Mb ChainFeRAM¿ design and power supply system design to meet HDD application are presented. Second, the concept of nonvolatile FeRAM cache and the simulated and measured HDD performance improvement are presented. The read/write bandwidth improvements to 1.12 times, 3.3 times and 1.9 times have been obtained by bench mark tests of PC Mark 05 and FD Bench v1.01, and by PC user data for 5 days, respectively. These results have been the same level of or more effective than the results of HDD rotational speed-up from 5400 rpm to 7200 rpm using DRAM cache. The write energy is reduced by 25% in PC Mark05 test.
Low-cost, high-performance package for a multi-chip MMIC module (2003)

A ceramic package for a multichip monolithic microwave integrated circuit (MMIC) has been developed which consists of a multilayer ceramic frame whose layers are metallized over most of both surfaces and cofired. The frame is metallized on all sides including inner and outer walls, and metal-to-ceramic hermetically sealed. Microstrip lines and through-wall microstrips with shielded walls for the RF I/O ports are designed for 50- Omega impedance. The insertion loss of each I/O port is less than 0.5 dB up to 33 GHz, with a complementary I/O isolation in excess of 30 dB. The package also provides 1.32:1 input VSWR (voltage standing-wave ratio). A 30-GHz-band multichip MMIC amplifier incorporating four GaAs MMICs was demonstrated using this ceramic package. A maximum gain of 19 dB is achieved with 1.5:1 input VSWR across the 20.5-to-32.5-GHz band.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Epithelial-to-Mesenchymal Transition and Autophagy Induction in Breast Carcinoma Promote Escape from T-cell–Mediated Lysis (2013)

Epithelial-to-mesenchymal transition (EMT) mediates cancer cell invasion, metastasis, and drug resistance, but its impact on immune surveillance has not been explored. In this study, we investigated the functional consequences of this mode of epithelial cell plasticity on targeted cell lysis by cytotoxic T lymphocytes (CTL). Acquisition of the EMT phenotype in various derivatives of MCF-7 human breast cancer cells was associated with dramatic morphologic changes and actin cytoskeleton remodeling, with CD24(-)/CD44(+)/ALDH(+) stem cell populations present exhibiting a higher degree of EMT relative to parental cells. Strikingly, acquisition of this phenotype also associated with an inhibition of CTL-mediated tumor cell lysis. Resistant cells exhibited attenuation in the formation of an immunologic synapse with CTLs along with the induction of autophagy in the target cells. This response was critical for susceptibility to CTL-mediated lysis because siRNA-mediated silencing of beclin1 to inhibit autophagy in target cells restored their susceptibility to CTL-induced lysis. Our results argue that in addition to promoting invasion and metastasis EMT also profoundly alters the susceptibility of cancer cells to T-cell-mediated immune surveillance. Furthermore, they reveal EMT and autophagy as conceptual realms for immunotherapeutic strategies to block immune escape.
Synthesis of some novel 4-substituted coumarins having antibacterial activity (Part-I) (2005)

Organic Research Laboratory. SS & LS Patkar College, Goregaon (W). Mumbai-400 062, India E-mail: ajayaaudi@rediffmail.com Fax : 91-22-28744755 <em>Manuscript received 13 February 2004, revised 1 September 2004, accepted 24 November 2004</em> Methyl coumarin-4-methyl acetate on bromination in dioxane or dichloroethane as solvent resulted in sidechain bromination giving a-bromo derivative. The same reaction when carried out in acetic acid resulted in selective synthesis of 3-bromo derivatives. The α-bromo derivatives on reaction with various urea derivatives give novel nitrogen heterocycles (imidazolones) substituted at 4-position of coumarin ring. The α-bromo derivatives when reacted with various 1.2-diamino compounds yielded fused 2-pyrazinones attached at 4-position of coumarin ring. Considering the potential biological activities of other 4-substituted coumarins and biological importance of imidazoles, pyrazines, triazines and oxazines in drug synthesis, the new heterocycles were examined for antibacterial properties and have shown positive results.
Tunable SERS using gold nanoaggregates on an elastomeric substrate (2013)

We report on the self-assembly of colloidal gold nanoparticles on a stretchable, elastomeric membrane, and the use of this membrane as a base substrate for far-field confocal Raman measurements. Surface-enhanced Raman scattering (SERS) enhancement for such a substrate was estimated as 10(6) to 10(7). Atomic force microscopy has been used to study the changes in nanoparticle topography when the membrane is stretched. The homogeneous strain defined by average relative motion of nanoparticles is approximately half the macroscopically-applied biaxial strain. The SERS intensity was maximized when the membrane was at rest (i.e. without stretch), and reduced as stretching was increased. Our measurements are consistent with theoretical and numerical SERS enhancements for the interstitial gap between two spheres. The data indicate that the resting gap between the spheres is 11 nm or 16 nm, using two theoretical models. This work represents progress towards particularly facile sample fabrication and in situ tuning techniques for SERS.