gebhart/patrepeval huggingface dataset:
Template method to improve brain segmentation from inhomogeneous brain magnetic resonance images at high fields (2010)

Magnetic resonance imaging of the brain at high fields (e.g. 3T) provides high resolution and high signal to noise ratio images suitable for a wide range of clinical applications. However, radiofrequency (or B <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">1</sub> ) inhomogeneity increases with the magnetic field and produces undesired intensity variations responsible for inaccuracies in quantitative analyses. A method to perform brain segmentation using T <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">1</sub> maps whose inhomogeneity was corrected using previously acquired B <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">1</sub> maps is described. A library of B <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">1</sub> maps was created and a method to compensate the T <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">1</sub> inhomogeneity using a B <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">1</sub> map from another subject (template) was developed. The performance of the template-based method was evaluated in 19 healthy volunteers. Our method produced significantly better segmentations than the retrospective N3 method and the one without B <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">1</sub> inhomogeneity correction.
Purification and Characterization of Ovarian Lh/Hcg and Prolactin Receptors (1987)

We have purified the luteinizing hormone (LH)/human choriogonadotropin (hCG) receptor to homogeneity by sequential affinity column on wheat germ lectin-Sepharose and hCG-Sepharose. The method was designed to allow also the purification of lactogen receptor from the initial starting material. Comparable purification of lactogen receptor can be attained using Con A-Sepharose as initial step. The purified LH/hCG receptor was identified as a single protein of Mr = 75,000 on SDS gel electrophoresis. The lactogen receptor is composed of two dissimilar active subunits of Mr 88,000 and 40,000, the latter probably being an integral part of the larger form. Comparison of Mr's derived from SDS gels with those from fast performance liquid chromatography suggested that the native LH holoreceptor is present in a dimeric form, while the lactogen receptor seems to be composed of aggregates that could represent dimeric or trimeric forms of holoreceptor Mr 80,000. Cross-linking studies performed after binding of hCG (radiolabeled in the individual subunits) to the purified LH/hCG receptor indicated that the hCG alpha-subunit undergoes predominant interaction with the receptor molecule. The influence of the beta-subunit in this interaction seems to occur mainly through its association with the alpha-subunit, presumably by conferring specificity to the alpha-subunit for its interaction with the receptor. The alpha-subunit, which is identical within species, has an important role in the receptor binding interaction and biological activity of glycoprotein hormones.
Parallelized STED fluorescence nanoscopy (2011)

We introduce a parallelized STED microscope featuring m = 4 pairs of scanning excitation and STED beams, providing m-fold increased imaging speed of a given sample area, while maintaining basically all of the advantages of single beam scanning. Requiring only a single laser source and fiber input, the setup is inherently aligned both spatially and temporally. Given enough laser power, the design is readily scalable to higher degrees of parallelization m.
An Efficient Synthesis of 5-Bromo-2-methoxy-6-methylaminopyridine-3-carboxylic Acid. (2000)

An efficient synthesis of 5-bromo-2-methoxy-6-methylaminopyridine-3-carboxylic acid (1), a carboxylic acid moiety of a potent dopamine D2 and D3 and serotonin-3 (5-HT3) receptors antagonist, (R)-5-bromo-N-(1-ethyl-4-methylhexahydro-1 ,4-diazepin-6-yl)-2-methoxy-6-methylaminopyridine-3-carboxamide, is described. Reaction of methyl 2,6-difluoropyridine-3-carboxylate (12) with methylamine in EtOH at -25 degrees C gave a mixture of methyl 2-fluoro-6-methylaminopyridine-3-carboxylate (13) and the regioisomer 14 in a ratio of 57 : 43. On the other hand, reaction of 12 and methyl 2,6-dichloropyridine-3-carboxylate (16) with sodium methoxide in tetrahydrofuran (THF) and CH2Cl2 provided the 2-methoxypyridine-3-carboxylic esters 20 and 23, respectively, as main products. Similar reaction of 16 in N,N-dimethylformamide (DMF) and MeOH proved to be highly regioselective for the 6-position. A much greater regioselectivity for substitution at the 6-position (>97%) was observed when 16 was treated with 4-methylbenzenethiolate anion in DMF (quantitative yield). After methoxylation of methyl 2-chloro-6-(4-methylbenzenethio)pyridine-3-carboxylate (25b) and successive oxidation of the 6-benzenethio moiety, nucleophilic substitution of the sulfoxide derivative 28 with methylamine gave the 6-methylamino derivative 8. Finally, bromination of 8 and alkaline hydrolysis produced the desired product 1 in an overall yield of 67%.
Perception is a funny thing (1996)

Thelma Harley recounts how she failed to quell a relative’s fears about her mother’s admission to hospital
Imino Diels–Alder adducts. XII. Two pyrano[3,2-<i>c</i>]quinolines (2006)

The crystal structures of pyranoquinolines 9-fluoro-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline, C18H18FNO, and 9-methyl-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline, C19H21NO, are isomorphous. In both structures, the pyran ring is exo to the six-membered N-heterocyclic ring formed in the cycloaddition step. The torsion angles across the phenyl linkage for the two structures are -91.2 (1) and -88.3 (2) degrees. The striking feature in both crystal packings is that they do not contain the expected conventional hydrogen bonds, in spite of the presence of good hydrogen-bonding functionalities. Possible C-H...pi interactions are, however, observed.
Putting the public (back) into public health: leadership, evidence and action (2018)

There is a strong evidence-based rationale for community capacity building and community empowerment as part of a strategic response to reduce health inequalities. Within the current UK policy context, there are calls for increased public engagement in prevention and local decision-making in order to give people greater control over the conditions that determine health. With reference to the challenges and opportunities within the English public health system, this essay seeks to open debate about what is required to mainstream community-centred approaches and ensure that the public is central to public health. The essay sets out the case for a reorientation of public health practice in order to build impactful action with communities at scale leading to a reduction in the health gap. National frameworks that support local practice are described. Four areas of challenge that could potentially drive an implementation gap are discussed: (i) achieving integration and scale, (ii) effective community mobilization, (iii) evidencing impact and (iv) achieving a shift in power. The essay concludes with a call to action for developing a contemporary public health practice that is rooted in communities and offers local leadership to strengthen local assets, increase community control and reduce health inequalities.
Maximum likelihood estimation of pure GARCH and ARMA-GARCH processes (2004)

We prove the strong consistency and asymptotic normality of the quasi-maximum likelihood estimator of the parameters of pure generalized autoregressive conditional heteroscedastic (GARCH) processes, and of autoregressive moving-average models with noise sequence driven by a GARCH model. Results are obtained under mild conditions.
Realization of button click feeling by use of ultrasonic vibration and force feedback (2009)

This paper presents a displaying method of button like click feeling using ultrasonic vibration with amplitude of a few micrometers. Artificial click feeling is displayed by recreating rapid change in force arising from buckling of a mechanical push button utilizing squeeze film effect. First, important factors for displaying click feeling were extracted by analyzing the relationship among force-stroke curve of mechanical buttons and perceived click feeling. Then, click feeling display system was constructed. In the system, stimulation are applied to the operators at both buckling and restitution point. Finally, by conducting several sensory evaluation experiments, it was verified that button like feeling can be displayed without recreating the stroke of mechanical buttons by use of ultrasonic vibration. It was also verified that various artificial click feeling can be displayed by controlling the multiple parameters of the system.
An Investigation of Evaporation Heat Transfer in Sintered Copper Wicks With Microgrooves (2006)

This paper presents an experimental study on the parameters that determine the thermal performance of sintered copper wicks with longitudinal micro grooves for heat pipe applications. The grooves, which provide passages to vent vapor, have a width in a range from 150 μm to 500 μm. The copper powder used here has a nominal diameter of 50 μm, which produces an effective pore radius of approximately 13 μm. The main wicks composed of pores and grooves present characteristics of bi-dispersed wick structures. Unlike traditional bi-dispersed wick structures, the sintered grooved wick structures provide undisrupted longitudinal liquid delivery passages and thus improve the boiling limit. Performance of the wick structures with distilled water was examined and the effects of the heat flux and groove geometries on the evaporation/boiling heat transfer performance were studied.
Mountaineering Risk, Safety and Security (2021)

The purpose of this paper is to explore some of the underlying mountaineering risks, safety and security issues found in the traditional activity known as mountaineering. The paper also highlights some of the commonly known and distinguished terms of mountaineering. Mountaineering is the art of moving through the mountains using a set of technical ‘mountaineering’ skills. In Europe, it is often called alpinism when done in the Alps, apart from known as trekking, hiking or even fell walking. It includes traditional outdoor rock climbing in a mountain setting, multi-day rock climbing, skiing, snow-shoeing, a multi-day journey in the mountains, whether camping or in lodges. Mountaineering is not necessarily restricted to the greater mountain ranges as many countries with low altitude mountains have many citizens that go ‘mountaineering’ inside their borders. There are no predetermined heights at which a hill becomes a mountain; many geographers state that a mountain is greater than 300m (1,000 feet) above sea level, but the Oxford English Dictionary puts the hill limit at 600m asl. Another consideration is the latitude of the ‘hills’. For example, the hills of Scotland, although the highest being Ben Nevis at 1345m asl, are considerably further north than the Pyrenees with its highest mountain, Pic Aneto, at 3404m asl. The arctic winds, the northern European winds and the south-westerly gales are as harsh as any found in the higher ranges.
Markov based decision support for cost-optimal response in security management (2022)

In this contribution, we introduce a prototype of a decision support tool for cost-optimal response in security management. The threat situation of a closed infrastructure, exposed to multiple threats, and the corresponding response actions are modeled by a continuous-time Markov decision process (CMDP). Since the CMDP cannot be solved exactly for large infrastructures, the response actions are determined from a heuristic, based on an index rule. The decision support tool's user interface displays the infrastructure's current threat state and proposes the heuristic response actions to the decision maker. In this way, global situation awareness can be enhanced and the decision maker is able to initiate an almost cost-optimal response action in short time.
p185erbB2 Binds to GRP94 in Vivo (1996)

Treatment of SKBr3 cells with benzoquinone ansamycins, such as geldanamycin (GA), depletes p185erbB2, the receptor tyrosine kinase encoded by the erbB2 gene. In the same cells, a biologically active benzoquinone photoaffinity label specifically binds a protein of about 100 kDa, and the ability of various GA derivatives to reduce the intracellular level of p185erbB2 correlates with their ability to compete with the photoaffinity label for binding to this protein. In this report, we present evidence that the approximately 100-kDa ansamycin-binding protein is GRP94. Membrane-associated p185erbB2 exists in a stable complex with GRP94. GA binding to GRP94 disrupts this complex, leading to degradation of pre-existing p185erbB2 protein, and resulting in an altered subcellular distribution of newly synthesized p185erbB2.
Rapid-Response Splicing Reporter Screens Identify Differential Regulators of Constitutive and Alternative Splicing (2010)

Bioactive compounds have been invaluable for dissecting the mechanisms, regulation, and functions of cellular processes. However, very few such reagents have been described for pre-mRNA splicing. To facilitate their systematic discovery, we developed a high-throughput cell-based assay that measures pre-mRNA splicing by utilizing a quantitative reporter system with advantageous features. The reporter, consisting of a destabilized, intron-containing luciferase expressed from a short-lived mRNA, allows rapid screens (<4 h), thereby obviating the potential toxicity of splicing inhibitors. We describe three inhibitors (out of >23,000 screened), all pharmacologically active: clotrimazole, flunarizine, and chlorhexidine. Interestingly, none was a general splicing inhibitor. Rather, each caused distinct splicing changes of numerous genes. We further discovered the target of action of chlorhexidine and show that it is a selective inhibitor of specific Cdc2-like kinases (Clks) that phosphorylate serine-arginine-rich (SR) protein splicing factors. Our findings reveal unexpected activities of clinically used drugs in splicing and uncover differential regulation of constitutively spliced introns.
Enzymic Properties of Three β-Glucosidases from<i>Aspergillus aculeatus</i>No. F-50 (1985)

The enzymic properties of three electrophoretically distinct β-glucosidases, β-glucosidase-1, -2 and -3, from Aspergillus aculeatus No. F-50 were investigated. β-Glucosidase-3 had a low optimum pH of 3.0, but the other two enzymes had optimum pHs of 4.0~4.5. Both β-glucosidase-1 and -2 were potently active not only on soluble cellooligosaccharides, such as cellotriose to cellohexaose, but also on insoluble cellooligosaccharide, of which the average degree of polymerization was 20. On the contrary, β-glucosidase-3 was only slightly active on the insoluble substrate. The combined use of either β-glucosidase-1 or -2 and endo-glucanase remarkably stimulated the hydrolysis of amorphous cellulose, yielding glucose only. But β-glucosidase-3 did not show such a synergistic effect, and the glucose content of the hydrolyzate was only about 60%.
Temporal and Spatial Localization of the Dentin Matrix Proteins During Dentin Biomineralization (2008)

Formation of bone and dentin are classical examples of matrix-mediated mineralization. The mineral phase is essentially the same in these two tissues and primarily consists of a carbonated hydroxyapatite, but the difference lies in the crystal size and shape. There are three components that are necessary for proper mineralization, namely the proper synthesis and secretion of the non-collagenous proteins (NCPs), self-assembly of the collagenous matrix, and delivery of calcium and phosphate ions to the extracellular matrix. Three major NCPs present in the dentin matrix are dentin matrix protein 1 (DMP1), dentin phosphophorin (DPP), and dentin sialoprotein (DSP). In this study, we show the temporal and spatial localization of these NCPs and correlate their expression with the presence of collagenous matrix and calcified deposits in developing mouse incisors and molars. DMP1, an acidic protein, is present predominantly at the mineralization front and in the nucleus of undifferentiated preodontoblast cells. DPP, the major NCP, is present in large amounts at the mineralization front and might function to regulate the size of the growing hydroxyapatite crystals. For the first time, we report the localization of DPP in the nucleus of preodontoblast cells, suggesting a signaling function during the odontoblast differentiation process. DSP is localized predominantly in the dentinal tubules at the site of peritubular dentin, which is highly mineralized in nature. Thus, the precise localization of DMP1, DPP, and DSP in the dentin tissue suggests that a concerted effort between several NCPs is necessary for dentin formation.
Conjunctivitis: diagnosis and management. (1999)

Diseases of the conjunctiva are diverse and need proper diagnosis, treatment and appropriate preventive measures.







Gram –ve rods (Pseudomonas) and white cells

Photo: Melville Matheson
Genome-wide association analysis reveals a<i>SOD1</i>mutation in canine degenerative myelopathy that resembles amyotrophic lateral sclerosis (2009)

Canine degenerative myelopathy (DM) is a fatal neurodegenerative disease prevalent in several dog breeds. Typically, the initial progressive upper motor neuron spastic and general proprioceptive ataxia in the pelvic limbs occurs at 8 years of age or older. If euthanasia is delayed, the clinical signs will ascend, causing flaccid tetraparesis and other lower motor neuron signs. DNA samples from 38 DM-affected Pembroke Welsh corgi cases and 17 related clinically normal controls were used for genome-wide association mapping, which produced the strongest associations with markers on CFA31 in a region containing the canine SOD1 gene. SOD1 was considered a regional candidate gene because mutations in human SOD1 can cause amyotrophic lateral sclerosis (ALS), an adult-onset fatal paralytic neurodegenerative disease with both upper and lower motor neuron involvement. The resequencing of SOD1 in normal and affected dogs revealed a G to A transition, resulting in an E40K missense mutation. Homozygosity for the A allele was associated with DM in 5 dog breeds: Pembroke Welsh corgi, Boxer, Rhodesian ridgeback, German Shepherd dog, and Chesapeake Bay retriever. Microscopic examination of spinal cords from affected dogs revealed myelin and axon loss affecting the lateral white matter and neuronal cytoplasmic inclusions that bind anti-superoxide dismutase 1 antibodies. These inclusions are similar to those seen in spinal cord sections from ALS patients with SOD1 mutations. Our findings identify canine DM to be the first recognized spontaneously occurring animal model for ALS.
Screening for obstructive sleep apnoea based on the electrocardiogram-the computers in cardiology challenge (2002)

The authors present a method of screening for obstructive sleep apnoea based on the electrocardiogram (ECG). The algorithm combines information from the ECG-derived respiration (EDR) signal and the RR interval tachogram. Power spectral features from the EDR signal were computed using the discrete harmonic wavelet transform, considering the power at the respiratory frequency and at frequencies below 0.1 Hz. Cycles of tachy/bradycardia (consistent with an arousal from sleep, as would be expected at the end of an episode of apnoea) were identified front the RR interval tachogram. Features were collated into minute-by-minute vectors and passed to a classifier. The algorithm correctly classified 81% of all minutes in the test database, with 29/30 patients correctly identified as apnoea or normal. Visual classification produced 92% correct classification, with all 30 patients correct.
Trends and challenges for wireless embedded DSPs (2004)

Programmable digital signal processors (DSP) are key enablers of rapidly expanding wireless markets. The primary handset market, subject to strict power constraints, also creates substantial process scaling barriers. Nonetheless, DSPs in particular, and hand-held ICs in general, are uniquely situated to utilize emerging circuit and SOC design techniques to their advantage, allowing more aggressive processes despite power barriers. This paper discusses some of the challenges and design opportunities.
The Thickness of Things: Exploring the Curriculum of Museums through Phenomenological Touch (2011)

Museums are places where material culture and specimens from nature provide opportunities to help visitors understand the lifeworld-sometimes of the ordinary, sometimes extraordinary, but always within the context of relationships to people, places and time. The encounter with museum objects provides the visitor with the range of possible human experiences, a personal sense of the life of another as it was lived, or simply provides access to the vital and fundamental aspects of being human-a phenomenological museum curriculum. Yet, despite the prospect of being a multi-sensory milieu, museums offer a peculiar provocation to the senses: don't touch. By framing the considerations of the need for touch within the museum, our intent is to explore the ideas of materiality, haptic touch, and the person-object transaction and its place within the museum curriculum. We apply these concepts to the idea of phenomenological touch in the museum setting as a new orientation for meaningful person-object transactions.
Highly Efficient Relativistic-Ion Generation in the Laser-Piston Regime (2004)

An intense laser-plasma interaction regime of the generation of high density ultrashort relativistic ion beams is suggested. When the radiation pressure is dominant, the laser energy is transformed efficiently into the energy of fast ions.
Push-content delivery method for accurate synchronization between broadcast programs and push content (2016)

We developed a push-content delivery method for integrated broadcasts on the Internet. To provide services that are highly synchronized between the broadcast programs and push content, we propose a new push-content delivery method that enables simultaneous reception of push content on all terminals.
A Flow‐Quench Apparatus for Cryoenzymic Studies (1980)

The flow-quench method was adapted to sub-zero conditions. Two apparatus were constructed: a rapid flow-quench apparatus (which take samples in the 5--300-ms time range) and a time delay flow-quench apparatus (0.5 s and longer). The apparatus were constructed so that the reagents are only in contact with chemically inert materials : glass, poly(tetrafluoroethylene) and poly(trifluorochloroethylene). The modified flow-quench apparatus were used to study the initial formation of creatine phosphate by creatine kinase at -15 degrees C in the time range 5 ms to 10 s. As at +4 degrees C [Travers, F., Barman, T. E. and Bertrand, R. (1979) Eur. J. Biochem. 100, 149--155], the time course of product formation was complex and consisted of three phase: a lag phase, a burst phase and the steady-state phase. The apparatus were also used to test chemically for reaction intermediates involving labile phosphate and phospho-enzyme complexes on the creatine kinase reaction pathway at -15 degrees C. Since neither type of intermediate could be detected down to 5 ms, this enzyme probably proceeds via a direct in-line type of mechanism.