gebhart/patrepeval huggingface dataset:
Understanding Biological Regulation Through Synthetic Biology (2018)

Engineering synthetic gene regulatory circuits proceeds through iterative cycles of design, building, and testing. Initial circuit designs must rely on often-incomplete models of regulation established by fields of reductive inquiry-biochemistry and molecular and systems biology. As differences in designed and experimentally observed circuit behavior are inevitably encountered, investigated, and resolved, each turn of the engineering cycle can force a resynthesis in understanding of natural network function. Here, we outline research that uses the process of gene circuit engineering to advance biological discovery. Synthetic gene circuit engineering research has not only refined our understanding of cellular regulation but furnished biologists with a toolkit that can be directed at natural systems to exact precision manipulation of network structure. As we discuss, using circuit engineering to predictively reorganize, rewire, and reconstruct cellular regulation serves as the ultimate means of testing and understanding how cellular phenotype emerges from systems-level network function.
Amperometric Biosensor for Total Histamine, Putrescine and Cadaverine using Diamine Oxidase (1996)

ABSTRACT Diamine oxidase was purified from porcine kidney to homogeneity and immobilized onto a porous preactivated nylon membrane. The enzymic membrane was attached to an amperometric electrode for determination of the total concentrations of histamine, cadaverine and putrescine accumulated in fish fillets during storage. For detecting hydrogen peroxide released, the working platinum electrode was poised at +400 mV vs Ag/AgCl to prevent electrode fouling as well as minimize electroactive interferences. The biosensor was linear up to 6 mM with a lower detection limit of 25 μM for the three substrates and steady state response was achieved within 1.5 min. The enzyme membranes were stable at 5°C for 2 mo and usable for at least 60 assays.
Speech Bandwidth Extension by Data Hiding and Phonetic Classification (2007)

Speech bandwidth extension can be defined as the deliberate process of expanding the frequency range (bandwidth) for speech transmission. Its significant advancement in recent years has led to the technology being adopted commercially in several areas including psychoacoustic bass enhancement of small loudspeakers and the high frequency enhancement of perceptually coded audio. In this paper, a data hiding method based on dither quantization is used for speech bandwidth extension. More specifically, the out-of-band information is encoded and embedded into the narrowband speech without degrading the quality of the bandlimited signal. At the receiver, when the out-of-band information is extracted from the hidden channel, it can be used to combine with the bandlimited signal, providing a signal with a wider bandwidth. To encode the out-of-band speech more efficiently, acoustic phonetic classification is employed to generate three linear prediction (LP) codebook. The simulation results show that compared with using non-classified codebook, the propose scheme have a better bandwidth extension performance in terms of log spectral distortion (LSD).
Delivery of therapeutic radioisotopes using nanoparticle platforms: potential benefit in systemic radiation therapy (2010)

Radiation therapy is an effective cancer treatment option in conjunction with chemotherapy and surgery. Emerging individualized internal and systemic radiation treatment promises significant improvement in efficacy and reduction of normal tissue damage; however, it requires cancer cell targeting platforms for efficient delivery of radiation sources. With recent advances in nanoscience and nanotechnology, there is great interest in developing nanomaterials as multifunctional carriers to deliver therapeutic radioisotopes for tumor targeted radiation therapy, to monitor their delivery and tumor response to the treatment. This paper provides an overview on developing nanoparticles for carrying and delivering therapeutic radioisotopes for systemic radiation treatment. Topics discussed in the review include: selecting nanoparticles and radiotherapy isotopes, strategies for targeting nanoparticles to cancers, together with challenges and potential solutions for the in vivo delivery of nanoparticles. Some examples of using nanoparticle platforms for the delivery of therapeutic radioisotopes in preclinical studies of cancer treatment are also presented.
Deletion of VCX-A due to NAHR plays a major role in the occurrence of mental retardation in patients with X-linked ichthyosis (2005)

X-linked ichthyosis (XLI) is often associated with a recurrent microdeletion at Xp22.31 due to non-allelic homologous recombination between the CRI-S232 low-copy repeat regions flanking the STS gene. The clinical features of these patients may include mental retardation (MR) and the VCX-A gene has been proposed as the candidate MR gene. Analysis of DNA from four XLI patients with MR by array-comparative genomic hybridization (array-CGH) on a 150 kb resolution X chromosome-specific array revealed a 1.5 Mb interstitial microdeletion with breakpoints in the CRI-S232 repeat sequences, each of which harbors a VCX gene. We demonstrate that the recombination sites in all four cases are situated in the 1 kb repeat unit 2 region present at the 3' ends of the VCX-A and VCX-B genes thereby deleting VCX-A and VCX-B1 but not VCX-B and VCX-C. Array-CGH with DNA of an XLI patient with MR and an inherited t(X;Y)(p22.31;q11.2) showed an Xpter deletion of 8.0 Mb resulting in the deletion of all four VCX genes and duplication of both VCY homologs. These data confirm the role of VCX-A in the occurrence of MR in XLI patients. Moreover, we propose a VCX/Y teamwork-dependent mechanism for the incidence of mental impairment in XLI patients.
Structure–function of the multifunctional Ca2+/calmodulin-dependent protein kinase II (2002)

Ca2+/calmodulin (CaM)-dependent protein kinase (CaMKII) is a ubiquitous mediator of Ca2+-linked signalling that phosphorylates a wide range of substrates to co-ordinate and regulate Ca2+-mediated alterations in cellular function. The transmission of information by the kinase from extracellular stimuli and the intracellular Ca2+ rise is not passive. Rather, its multimeric structure and autoregulation enable this enzyme to participate actively in the sensitivity, timing and location of its action. CaMKII can: (i) be activated in a Ca2+-spike frequency-dependent manner; (ii) become independent of its initial Ca2+/CaM activators; and (iii) undergo a ‘molecular switch-like’ behaviour, which is crucial for certain forms of learning and memory. CaMKII is derived from a family of four homologous but distinct genes, with over 30 alternatively spliced isoforms described at present. These isoforms possess diverse developmental and anatomical expression patterns, as well as subcellular localization. Six independent catalytic/autoregulatory domains are connected by a narrow stalk-like appendage to each hexameric ring within the dodecameric structure. Ca2+/CaM binding activates the enzyme by disinhibiting the autoregulatory domain; this process initiates an intra-holoenzyme autophosphorylation reaction that induces complex changes in the enzyme's sensitivity to Ca2+/CaM, including the generation of Ca2+/CaM-independent (autonomous) activity and marked increase in affinity for CaM. The role of CaMKII in Ca2+ signal transduction is shaped by its autoregulation, isoenzymic type and subcellular localization. The molecular determinants and mechanisms producing these processes are discussed as they relate to the structure—function of this multifunctional protein kinase.
Hematology : a combined theoretical & technical approach (1989)

An outstanding text of basic hematology for medical technologists and medical students. This volume offers excellent coverage of blood development and renal and hepatic functions, as well as clinical considerations. It provides basic coverage of hematology, disease and lab techniques, and integrates automation and instrumentation into chapters. Features a separate chapter on quality assurance.
Effectiveness of The Mathematics Student Development Center in TIP Manila (2000)

This study was conducted to determine the effectiveness of the Mathematics Student Development Center (MSDC) in TIP - Manila. Specifically, the study sought to determine how the tutees perceived the tutors in terms of: the delivery of the subject matter; organization of topics, responsiveness and relevance of topics. Furthermore, it sought to determine if there's a significant gain between the pretest and the posttest scores of the first year students who were exposed to Mathematics Student Development Center. Likewise, this study sought to evaluate the percent passing rate in Math subjects of students exposed to Mathematics Student Development Center. The quasi-experimental type of research was used with questionnaires, pretests and posttests administered to gather the needed data. There were twenty - seven (27) respondents involved in the study who were attending the Math Clinic and Saturday Tutorials and who were taking their College Algebra class during the first semester of school year 2007 - 2008. The data collected were analyzed using the statistical tools such as weighted mean and t - test. Findings revealed that the tutees very strongly agreed that the tutors were effective and efficient in terms of the delivery of the subject matter, organization of the topics, and responsiveness and relevance of topics. Also, a significant gain was noted on the pretest and the posttest scores of the first year students who were exposed to Mathematics Student Development Center as supported by the computed t - value of 11.13 that was higher than the tabular value of 2.021 with 52 degrees of freedom at 0.05 level. Furthermore, twenty six (26) out of the twenty seven (27) student-respondents got passing grades in College Algebra with a mean grade of 2.97. This showed that the student-respondents who were exposed to MSDC had a very high passing percentage. In the light of the findings, it was concluded that the tutees perceived the tutors as effective and efficient in terms of the delivery of the subject matter, organization of the topics, and responsiveness and relevance of topics. There was a significant gain between the pretest and the posttest scores of the first year students who were exposed to MSDC. The percent passing rate of students' exposure in the MSDC was 96.30%, which is considered very high. The MSDC in TIP - Manila can be considered effective and efficient.
Understanding Visualization by Understanding Individual Users (2012)

Visualization is often seen as a tool to support complex thinking. Although different people can have very different ways of approaching the kind of complex task that visualizations support, as researchers and designers we still rarely consider individual differences in creating and evaluating visualizations. This article reviews recent research on individual differences in visualization and human-computer interaction, showing that both cognitive abilities and personality profiles might significantly affect performance with these tools. The study of individual differences has led to the conclusion that advances in this important area in visualization will require more focused research. Specifically, we must isolate the cognitive factors that are relevant to visualization and the design factors that make one visualization more suited to a user than another. In doing so, we could increase our understanding of the visualization user and reshape how we approach design and evaluation.
Online SLAM with Any-time Self-calibration and Automatic Change Detection (2014)

A framework for online simultaneous localization, mapping and self-calibration is presented which can detect and handle significant change in the calibration parameters. Estimates are computed in constant-time by factoring the problem and focusing on segments of the trajectory that are most informative for the purposes of calibration. A novel technique is presented to detect the probability that a significant change is present in the calibration parameters. The system is then able to re-calibrate. Maximum likelihood trajectory and map estimates are computed using an asynchronous and adaptive optimization. The system requires no prior information and is able to initialize without any special motions or routines, or in the case where observability over calibration parameters is delayed. The system is experimentally validated to calibrate camera intrinsic parameters for a nonlinear camera model on a monocular dataset featuring a significant zoom event partway through, and achieves high accuracy despite unknown initial calibration parameters. Self-calibration and re-calibration parameters are shown to closely match estimates computed using a calibration target. The accuracy of the system is demonstrated with SLAM results that achieve sub-1% distance-travel error even in the presence of significant re-calibration events.
Silicone Liner‐Free Pressure‐Sensitive Adhesive Labels (2008)

Abstract Pressure‐sensitive adhesives (PSA) were microencapsulated using simple and complex coacervation and aminoplaste. The microcapsules thus prepared were characterized by FTIR spectroscopy, particle size distribution, rheological behavior, and peeling tests. The microcapsules were isolated and found to be out of sticky indicating that the PSAs were indeed encapsulated. The prepared suspensions were deposited at the surface of a paper sheets and the dried labels were then pressed against each other. The ensuing complex was then characterized in terms of peeling forces and showed that the encapsulation using aminoplaste technique of a commercial PSA yielded peel energy of 170 J · m −2 , which constitutes the recovering of about 68% of the adhesive power of the original nonencapsulated PSA. magnified image
Surface chemical heterogeneity modulates silica surface hydration (2018)

An in-depth knowledge of the interaction of water with amorphous silica is critical to fundamental studies of interfacial hydration water, as well as to industrial processes such as catalysis, nanofabrication, and chromatography. Silica has a tunable surface comprising hydrophilic silanol groups and moderately hydrophobic siloxane groups that can be interchanged through thermal and chemical treatments. Despite extensive studies of silica surfaces, the influence of surface hydrophilicity and chemical topology on the molecular properties of interfacial water is not well understood. In this work, we controllably altered the surface silanol density, and measured surface water diffusivity using Overhauser dynamic nuclear polarization (ODNP) and complementary silica-silica interaction forces across water using a surface forces apparatus (SFA). The results show that increased silanol density generally leads to slower water diffusivity and stronger silica-silica repulsion at short aqueous separations (less than ∼4 nm). Both techniques show sharp changes in hydration properties at intermediate silanol densities (2.0-2.9 nm<sup>-2</sup>). Molecular dynamics simulations of model silica-water interfaces corroborate the increase in water diffusivity with silanol density, and furthermore show that even on a smooth and crystalline surface at a fixed silanol density, adjusting the spatial distribution of silanols results in a range of surface water diffusivities spanning ∼10%. We speculate that a critical silanol cluster size or connectivity parameter could explain the sharp transition in our results, and can modulate wettability, colloidal interactions, and surface reactions, and thus is a phenomenon worth further investigation on silica and chemically heterogeneous surfaces.
Improvement in risk stratification with the combination of the tumour marker antigen carbohydrate 125 and brain natriuretic peptide in patients with acute heart failure (2010)

In patients admitted with AHF, CA125 added prognostic value beyond the information provided by BNP, and thus, their combination enables better 6-month risk stratification.
Tunable Nanoscale Localization of Energy on Plasmon Particle Arrays (2007)

We demonstrate experimentally that plasmon resonant nanoparticle chains exhibit a nanoscale localized response to unfocused light that can be controlled by tuning the incident wavelength. Confocal microscopy shows that field concentrates on just a few nanoparticles at either the front or back side of the plasmon chains. Our experiments clearly demonstrate that plasmon chains are nanoscale receivers and concentrators of light analogous to multielement radio wave antennas. This analogy inspires new design rules for many other photonic functionalities, like guiding and redirecting light at the nanoscale.
Synthesis and Efficacy of Square Planar Copper Complexes Designed to Nucleate .beta.-Sheet Structure (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis and Efficacy of Square Planar Copper Complexes Designed to Nucleate .beta.-Sheet StructureJoel P. Schneider and Jeffery W. KellyCite this: J. Am. Chem. Soc. 1995, 117, 9, 2533–2546Publication Date (Print):March 1, 1995Publication History Published online1 May 2002Published inissue 1 March 1995https://pubs.acs.org/doi/10.1021/ja00114a016https://doi.org/10.1021/ja00114a016research-articleACS PublicationsRequest reuse permissionsArticle Views1130Altmetric-Citations110LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (2)»Supporting Information Supporting Information Get e-Alerts
Cause-specific mortality in individuals with severe alpha 1-antitrypsin deficiency in comparison with the general population in Sweden (2016)

PiZZ individuals had a reduced mortality risk of IHD. Mortality due to respiratory, hepatic disease, diverticulitis, and pulmonary embolism was markedly increased compared with the age- and sex-matched Swedish population.
Rotating behind Privacy: An Improved Lightweight Authentication Scheme for Cloud-based IoT Environment (2021)

The advancements in the internet of things (IoT) require specialized security protocols to provide unbreakable security along with computation and communication efficiencies. Moreover, user privacy and anonymity has emerged as an integral part, along with other security requirements. Unfortunately, many recent authentication schemes to secure IoT-based systems were either proved as vulnerable to different attacks or prey of inefficiencies. Some of these schemes suffer from a faulty design that happened mainly owing to undue emphasis on privacy and anonymity alongside performance efficiency. This article aims to show the design faults by analyzing a very recent hash functions-based authentication scheme for cloud-based IoT systems with misunderstood privacy cum efficiency tradeoff owing to an unadorned design flaw, which is also present in many other such schemes. Precisely, it is proved in this article that the scheme of Wazid et al. cannot provide mutual authentication and key agreement between a user and a sensor node when there exists more than one registered user. We then proposed an improved scheme and proved its security through formal and informal methods. The proposed scheme completes the authentication cycle with a minor increase in computation cost but provides all security goals along with privacy.
Invasiveness of Primary and Secondary Brain Tumors In Vitro Correlated with Clinical Results (1992)

One hundred fifty-one tumor fragments were collected in the neurosurgical operating amphitheater immediately after removal. Small tumor fragments were transferred into culture flasks and cultured until a confluent monolayer was formed by the outgrowing cells. Flaps of these cell monolayers were mechanically scraped from the culture flasks and confronted with embryonic chick heart tissue in vitro. The evolution of the confrontations was followed for a week. Histological analysis of the confrontations demonstrated three different morphological patterns of interaction between the heart tissue and the tumor-derived cells: 1) progressive engulfment of the tumor-derived cells by the heart tissue (Type I), 2) survival of both the heart tissue and the tumor-derived cells (Type II), and 3) progressive replacement of the heart tissue by tumor-derived cells (Type III). The replacement of the heart tissue by tumor-derived cells was only observed in cells originating from malignant tumors that were invasive and metastatic in vivo. Thus, invasiveness in confrontation culture is correlated with malignancy in vivo.
Preliminary communication: Magnetic-field-induced Freedericksz transition of a planar aligned liquid crystal doped with porphyrinatozinc(II): influence of the substituent of the porphyrin ring (2000)

Abstract The magnetic-field-induced Freedericksz transition of a planar aligned nematic liquid crystal doped with 1.0 wt % of a diamagnetic porphyrinatozinc(II) has been studied. It was observed that 5CB doped with 1.0 wt % of derivatives of Zn(II)TPP-5,10,15,20-tetraphenyl-porphyrinatozinc(II) - which have different substituents in one phenyl ring display sharp Freedericksz transition curves and dramatically decrease the critical magnetic field compared with pure 5CB.
A bioluminescent orthotopic mouse model of human osteosarcoma that allows sensitive and rapid evaluation of new therapeutic agents In vivo. (2009)

Osteosarcoma (OSA) is the most common primary malignant bone tumor in children, 30% of whom develop lung metastases despite aggressive treatment. Our objective was to develop a mouse model of OSA for preclinical studies that (i) incorporates the natural history of OSA including tumor growth in bone and development of lung metastasis and (ii) is amenable to non-invasive detection methods. A human OSA cell line that expresses high levels of luciferase was created. Following subcutaneous injection, nine out of ten mice showed tumor growth. Eight out of ten mice showed tumor growth following orthotopic injection into the proximal tibia. Thirty percent of mice showed pulmonary metastasis by bioluminescent imaging eight to 10 weeks following orthotopic injection. Animals receiving cisplatin treatment showed reduced tumor volume compared to animals treated with vehicle alone. This model allows real-time detection of tumors and can be used to study mechanisms of OSA metastasis and test new therapeutic agents.
An interpretable machine learning model for predicting the optimal day of trigger during ovarian stimulation (2022)

This study demonstrates that it is possible to develop an interpretable machine learning model for optimizing the day of trigger. Using our model has the potential to improve outcomes for many in vitro fertilization patients.