gebhart/patrepeval huggingface dataset:
Extracellular Vesicles in the Pathogenesis of Viral Infections in Humans (2020)

Most cells can release extracellular vesicles (EVs), membrane vesicles containing various proteins, nucleic acids, enzymes, and signaling molecules. The exchange of EVs between cells facilitates intercellular communication, amplification of cellular responses, immune response modulation, and perhaps alterations in viral pathogenicity. EVs serve a dual role in inhibiting or enhancing viral infection and pathogenesis. This review examines the current literature on EVs to explore the complex role of EVs in the enhancement, inhibition, and potential use as a nanotherapeutic against clinically relevant viruses, focusing on neurotropic viruses: Zika virus (ZIKV) and human immunodeficiency virus (HIV). Overall, this review's scope will elaborate on EV-based mechanisms, which impact viral pathogenicity, facilitate viral spread, and modulate antiviral immune responses.
Hysteresis in Asphaltene Precipitation and Redissolution (2005)

The precipitation and redissolution of asphaltenes from mixtures of Athabasca bitumen and n-heptane was measured over time in both air and nitrogen atmospheres at 23 °C. In air, it appears that oxidation of the bitumen increased the asphaltene yield for as long as the experiments were conducted; that is, for several months. When oxidation effects are excluded, asphaltene precipitation and redissolution both appear to reach steady state within 24 h. A hysteresis between asphaltene precipitation and redissolution was also observed in both atmospheres. The hysteresis was exaggerated in the air atmosphere due to oxidation effects. In both air and nitrogen atmospheres, complete reversibility was attained when the heptane-to-bitumen ratio was reduced to 1.5 cm3/g. The hysteresis is attributed to an energy barrier to asphaltene dissociation. As more heptane is added, the asphaltenes likely self-associate to higher apparent molar masses. When the heptane is removed, the asphaltenes may remain in the higher association state and hence precipitate to a greater extent than before at a given heptane-to-bitumen ratio. If the system is heated, the asphaltene yields return to their original values, indicating that the original association state can be restored.
Comunicare la città, comunicare il territorio (2001)

Il design della comunicazione si misura con i brand. Nell'articolo si descrivono alcune sperimentazioni realizzate nella nuova facoltà di design come quella del racconto sulla cultura del caffè con Lavazza.
Probing Apoptotic Caspase Allostery and Exosite Interactions for Alternative Regulation (2021)

Programmed cell death, or apoptosis is a critical homeostatic pathway that monitors the balance of cell life and death. Apoptosis is regulated by a class of enzymes known as the cysteine aspartic proteases, or the caspases. The 12 human caspases that play important roles in the progression and regulation of apoptosis and inflammation. Caspases are tightly regulated by numerous factors including enzymatic activation, post-translational modifications, metal ligand binding, and protein modulation. Aberrant caspase activation and regulation has been implicated in the progression of numerous diseases such as proliferative diseases and neurodegeneration. The deeply entwined nature of caspases and apoptosis makes them interesting targets for therapeutic intervention of apoptotic diseases. However, the highly conserved fold and overlapping active site specificities makes the development of specific caspase modulators difficult. Therefore, it is critical to understand the mechanistic details of each caspases function, dynamics and specificity to further differentiate these homologous enzymes and uncover new scaffolds for select targeting of these enzymes. In this work, we aim to identify distal residues that comprise protein exosites which mediate the recruitment of proteins for enzymatic hydrolysis. First we developed an evolved specificity caspase that may be utilized for the identification of specific substrate that utilize exosite for caspase recognition and recruitment. Next we probed the details of caspase regulation by zinc validating the emerging signaling atom as an essential regulator of the caspases. Next we identified a putative exosite of caspase-6 located within the N-terminal domain that is essential for protein substrate recruitment and also modulates the dynamics of unique 130’s helix to strand interconversion of the enzyme. Lastly, we identified an active site adjacent inhibitor of caspase-6 that takes advantage of a unique evolutionarily conserved cysteine of caspase-6. Modulation of this distal cysteine serves as an anchor for the most selective, potent and cell permeable inhibitor of caspase-6 to date. The identification of exosites provides new scaffolds for development of specific inhibitors that take advantage of the unique characteristics of this family of enzymes. Interestingly, uncovering the details of protein exosites also provides the unique opportunity block substrate hydrolysis by targeting either the enzyme exosite or the substrate exosite, thereby expanding the chemical space for effective modulation and therapeutic intervention.
Protection From Jp-8 Jet Fuel Induced Immunotoxicity By Administration of Aerosolized Substance P (1997)

Chronic exposure to jet fuel has been shown to cause human liver dysfunction, emotional dysfunction, abnormal electroencephalograms, shortened attention spans, and decreased sensorimotor speed. The United States Air Force has decided to implement the widespread use of JP-8 jet fuel in its operations, although a thorough understanding of its potential effects upon exposed personnel is unclear. Exposure to potential environment toxicants such as JP-8 may have significant effects on host physiology. Previous studies in mice have shown that short-term, low concentration JP-8 exposure had significant effects on the immune system; e.g., decreased viable immune cell numbers, decreased immune organ weights, and loss on immune function that persisted for extended periods of time (i.e., up to 4 weeks post-exposure). Previous studies have shown that JP-8 induced pulmonary dysfunction was associated with a decrease in levels of the neuropeptide substance P (SP) in lung lavage fluids. It was found that administration of aerosolized SP was able to protect exposed animals from such JP-8 induced pulmonary changes. In the current study, aerosolized SP was analyzed for its effects on JP-i induced immunotoxicity in exposed mice. It was observed that SP administration could protect JP-8 exposed animals from losses of viable immune cell numbers, but not losses in immune organ weights. Further, exposure of animals to SP inhibitors generally increased the immunotoxicity of JP-8 exposure. SP appeared to act on all immune cell populations equally as analyzed by flow cytometry, as no one immune cell population appeared to be preferentially protected by SP. Also, SP administration was capable of protecting JP-8 exposed animals from loss of immune function at all concentrations of JP-8 utilized (250-2500 mg/m3). Significantly, SP only needed to be administered for 15 minutes after JP-8 exposure, and was active at both 1 microM and 1 nM concentrations. Thus, SP administration appears to be a relatively simple and efficient means to reverse the immunotoxicity due to hydrocarbon exposure.
$\mathcal{PT}$-symmetric electronics (2012)

We show both theoretically and experimentally that a pair of inductively coupled active LRC circuits (dimer), one with amplification and another with an equivalent amount of attenuation, display all the features which characterize a wide class of non-Hermitian systems which commute with the joint parity-time PT operator: typical normal modes, temporal evolution, and scattering processes. Utilizing a Liouvilian formulation, we can define an underlying PT-symmetric Hamiltonian, which provides important insight for understanding the behavior of the system. When the PT-dimer is coupled to transmission lines, the resulting scattering signal reveals novel features which reflect the PT-symmetry of the scattering target. Specifically we show that the device can show two different behaviors simultaneously, an amplifier or an absorber, depending on the direction and phase relation of the interrogating waves. Having an exact theory, and due to its relative experimental simplicity, PT-symmetric electronics offers new insights into the properties of PT-symmetric systems which are at the forefront of the research in mathematical physics and related fields.
Smart sensor demonstration payload (2010)

This paper discusses smart sensors which are a critical element to monitoring, control, and evaluation processes as needed to support ground based testing for rocket engine test. Sensor applications involve tens to thousands of sensors; their reliable performance is critical to achieve overall system goals. These system engineering trade-offs include the familiar constraints of power, signal conditioning, cabling, reliability, and mass, and now include considerations such as spectrum allocation and interference for wireless sensors.
Effect of Automation Instructions and Vehicle Control Algorithms on Eye Behavior in Highly Automated Vehicles (2019)

Increasingly vehicle automation may convey greater capability than it actually possesses. The emergence of highly capable vehicle automation (e.g., SAE Level 4) and the promise of driverless vehicles in the near future can lead drivers to inappropriately cede responsibility for driving to the vehicle with less capable automation (e.g., SAE Level 2). This inappropriate reliance on automation can compromise safety, and so we investigated how algorithms and instructions might mitigate overreliance. Seventy-two drivers, balanced by gender, between the ages of 25 and 55, participated in this study using a fixed-base driving simulator. Drivers were exposed to one of three vehicle steering algorithms: lane centering, lane keeping, or an adaptive combination. A gaze tracker was used to track eye glance behavior. While automation was engaged, participants were told they could interact with an email sorting task on a tablet positioned near the center stack. Changes in roadway demand—traffic approaching in the adjacent lane—varied across the drive. Instructions indicating the driver was responsible, in combination with the adaptive algorithm, led drivers to be particularly attentive to the road as the traffic approached them. These results also have implications for evaluating more capable automation (SAE Levels 4 and 5), where drivers need not attend to the road: unnecessary attention to roadway demands might indicate lack of trust and acceptance of control algorithms that guide driverless vehicles.
Subcellular localization of the first three enzymes of the ommochrome synthetic pathway in <i>Drosophila melanogaster</i> (1974)

Abstract The subcellular distribution of the first three enzymes in the ommochrome eye pigment pathway were studied by means of differential centrifugation. Tryptophan pyrrolase and kynurenine formamidase are found in the soluble fraction of the homogenate. A relatively small amount of tryptophan pyrrolase, 10 to 15% of the total, is sedimentable. The head region of pupae contains approximately 15% of the total activity of the animal. The subcellular distribution of the activity of the head is not appreciably different from the distribution of activity in the rest of the animal. Therefore no evidence suggests that the enzyme exists in a particulate form specifically in the head. Kynurenine hydroxylase is judged to be a mitochondrial enzyme on the basis of its co‐sedimentation with particles containing cytochrome oxidase and by the demonstration of mitochondria in the sedimented fractions containing kynurenine hydroxylase activity. Kynurenine hydroxylase was judged not to be present in pigment granules since developmental and genetic changes in pigment granule morphology do not affect the sedimentation of kynurenine hydroxylase containing particles. These results on the three enzymes lead us to conclude that they are not organized into a single multi‐enzyme complex as has been previously suggested. A consideration of the six ommochrome deficient mutants indicates that four of these can be explained by existing data. A hypothesis which does not involve enzyme complexes is presented to account for the remaining two.
Thermal Modeling for System-in-a-Package Based on Embedded Chip Structure (2006)

This paper presents a thermal modeling for a new packaging concept, known as system-in-a-package, in which embedded chip technology is used. This structure fulfills the requirements for high integration and high density, while at the same time, would expect to be poor thermal performance because of the encapsulation on the chip. Thermal modeling here aims to give the first insight to characterize the thermal performance for such structure under steady and transient condition. It shows that substrates with good thermal conductivity help to keep the temperature on the chip at a reasonable level. For poorly conductive substrates, heat only conduct through copper tracks. Epoxy around the chip also plays an important role when the thermal conductivity of the substrate is good but is of less importance when the substrate has poor conductivity. The thickness of copper had large influence on the thermal resistance, while the thickness of chip does not effect at all. The thermal resistance is reduced considerably when the air velocity is increased.
Chp‐1 and melusin, two CHORD containing proteins in vertebrates (2003)

Melusin is a muscle specific protein required for heart hypertrophy in response to mechanical overload. Here we describe a protein 63% homologous to melusin, named chp-1, expressed in all tissues tested, including muscles, and highly conserved from invertebrates to human. Both proteins are characterized in their N-terminal half by a tandemly repeated zinc binding 60 amino acid domain with a motif of uniquely spaced cysteine and histidine residues. These motives are highly conserved from plants to mammals and have been recently named CHORD (for cysteine and histidine rich domain) domains. At the C-terminal end melusin contains a calcium binding stretch of 30 acidic amino acid residues which is absent in chp-1. While invertebrate genome contains only one gene coding for a chp-1 homolog, two genes coding for CHORD containing proteins (chp-1 and melusin) are present in vertebrates. Sequence analysis suggests that the muscle specific CHORD containing protein melusin originated by a gene duplication event during early chordate evolution.
Dynamic Collaborative Cloud Service Platform: Opportunities and Challenges (2010)

This letter presents a model for a dynamic collaboration (DC) platform among cloud providers (CPs) that prevents adverse business impacts, cloud vendor lock-in and violation of service level agreements with consumers, and also offers collaborative cloud services to consumers. We consider two major challenges. The first challenge is to find an appropriate market model in order to enable the DC platform. The second is to select suitable collaborative partners to provide services. We propose a novel combinatorial auction-based cloud market model that enables a DC platform among CPs. We also propose a new promising multi-objective optimization model to quantitatively evaluate the partners. Simulation experiments were conducted to verify both of the proposed models.
Near-infrared (NIR) spectroscopy. A new method for arthroscopic evaluation of low grade degenerated cartilage lesions. Results of a pilot study (2007)

NIRS can be used to distinguish between ICRS grade 1 lesions and healthy cartilage during arthroscopic surgeries. The results of this clinical study demonstrate the potential of NIRS to objectify classical arthroscopic grading systems.
Characteristics of Na+ channels and Cl‐ conductance in resealed muscle fibre segments from patients with myotonic dystrophy. (1990)

1. Electrical and contractile properties of resealed fibre segments were investigated by a variety of in vitro techniques. The preparations were removed from skeletal muscles of normal subjects and of eight patients with myotonic dystrophy. 2. Several hours after removal, fibre segments from normal subjects and those patients in whom myotonia was the primary symptom had resting membrane potentials of approximately -80 mV. In contrast, fibre segments obtained from patients in whom muscle dystrophy was more expressed were depolarized (-60 to -70 mV). 3. Contractions induced in fibre segments of myotonic muscle which had normal potentials were characterized by slowed relaxation which was due to electrical after-activity. 4. After single stimuli, long-lasting (3-100) runs of action potentials were recorded intracellularly from the myotonic muscle. In some of these fibre segments complex repetitive discharges were observed: multiple sites of locally gated currents were identified. 5. The three-electrode voltage clamp was used to determine the total membrane conductance, gm, and the ion component conductances. All fibres of a particular patient had similar conductances. However, the Cl- conductance varied from patient to patient from normal (74% of gm) to low values (30% of gm). The K+ conductance was normal in all fibres of all patients. 6. The patch-clamp technique was used to record currents through single Na+ channels of the sarcolemma. After treatment of the fibre segments with collagenase gigaohm seals were routinely obtained. The rate of success was greater when using the cell-attached mode than the inside-out mode. 7. Sodium channel currents were elicited by depolarizing voltage steps which produced an initial burst of Na+ channel openings. Up to ten channels were activated simultaneously when the patch was depolarized to potentials more positive than -30 mV. The Na+ channels re-opened very rarely in controls. The macroscopic sodium current, INa, was reconstructed by averaging depolarizing pulses. The time constant of rapid decay of INa reflecting macroscopic inactivation, the onset of INa and the amplitude of INa were voltage dependent. The mean amplitude of the current produced by re-openings was on average only 0.11 +/- 0.04% of the amplitude of the peak current. 8. Late openings of the Na+ channels were frequent in patches on the myotonic fibre segments. The amplitude of the current produced by re-openings was as high as about 0.75 +/- 0.11% of the amplitude of the peak current.(ABSTRACT TRUNCATED AT 400 WORDS)
The improvement of ohmic contact of Ti/Al/Ni/Au to AlGaN/GaN HEMT by multi-step annealing method (2009)

A multi-step rapid thermal annealing process of Ti/Al/Ni/Au was investigated for ohmic contact of AlGaN/GaN high electron mobility transistor(HEMT).The samples were studied by Transmission Line Model(TLM),Scanning Electron Microscopy(SEM) and Auger Electron Spectroscopy(AES) measurements. By the multi-step annealing process, the specific contact resistance was decreased and the surface morphology was improved. The AES measurements showed that the limitation indiffusion of Au and outdiffusion of Al were account for the surface morphology improvement.
Acoustic and Ultrasonic Detection With Radio-Frequency Encoded Fiber Laser Sensors (2016)

Fiber lasers have been exploited as sensors for the detection of extremely weak mechanical parameters, by monitoring the radio-frequency beat signal between orthogonal polarized lasing modes. This sensor scheme has presented advantages including advanced measurement capability, low cost and convenience in signal demodulation, resistance to environmental disturbances, and intrinsic multiplexing capability. Here, we demonstrate the capability of the fiber laser sensors for the detection of low-frequency (<;2 kHz) acoustic waves and ultrasound signals at frequency ranges >1 MHz (denoted as “high frequency” in this paper) and around 200 kHz (denoted as “medium frequency”), toward different applications. For low-frequency acoustic sensing, the detection limit reaches 74 μPa/Hz <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">1/2</sup> at 1 kHz which is comparable to the zeroth-order sea noise. For high-frequency ultrasound detection, the laser sensor presents a detection capability of 40 Pa over 50 MHz bandwidth, which allows its application as an ultrasound detector in photoacoustic (PA) imaging. Preliminary three dimensional PA imaging is presented, by means of time-to-space reconstruction. Ultrasound detection with a polymer-packaged fiber laser at 100 kHz range is demonstrated, which could find applications in underwater search and targeting. Its imaging capability is also exhibited.
Measurement of Tomato Maturity by Delayed Light Emission (1985)

Tomatoes were classified into three stages of maturity on the basis of visual color appearance and evaluated for differences in intensity of delayed light emission (DLE) and chlorophyll and carotenoid pigment contents. High correlations between DLE and chlorophyll show that as tomatoes ripened, DLE intensity and chlorophyll decreased and carotenoids increased. Results showed that DLE has a high potential for use in the automated sorting of fresh market tomatoes into categories of maturity.
Analysis of single-platform passive emitter location with terrain data (2001)

Frequency measurements made at a moving platform can be used to locate an emitter. An error ellipsoid analysis is used to compare the performance under three levels of a priori information on the emitter's altitude: (1) no knowledge, (2) terrain data, and (3) complete knowledge of the emitter's altitude. The analysis is performed for two simple platform paths that provide frequency measurements that are approximately time reversed versions of one another. When no a priori knowledge is available there is little difference between the performance when the platform maneuvers on a concave circular path or on a convex circular path and the performance depends very Little on the platform altitude. However, when some a priori altitude information is available the performance is markedly different on the two paths and is highly dependent on the platform altitude. Thus, this analysis provides the unexpected result that for seemingly similar platform paths, the performance can vary markedly when the emitter altitude is assumed known. Also, an interesting result is that for some cases it is possible to achieve better x-y accuracy when using terrain data than when the emitter's z location is known, because the terrain data provides terrain slope information. These cases are characterized in terms of the terrain slope at the emitter.
UWB pulse propagation into human tissues (2013)

In this paper the propagation of a UWB pulse into a layered model of the human body is studied to characterize absorption and reflection of the UWB signal due to the different body tissues. Several time behaviours for the incident UWB pulse are considered and compared with reference to the feasibility of breath and heartbeat activity monitoring. Results show that if the UWB source is placed far from the human body, the reflection coming from the interface between air and skin can be used to detect the respiratory activity. On the contrary, if the UWB source is placed close to the human body, a small reflection due to the interface between the posterior lung wall and the bone, which is well distanced in time from the reflections due to the first layers of the body model, can be used to detect lung and heart changes associated with the cardio-respiratory activity.
Runtime Checking of MPI Applications with MARMOT (2005)

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