gebhart/patrepeval huggingface dataset:
Pili and lipopolysaccharide of Pseudomonas aeruginosa bind to the glycolipid asialo GM1 (1994)

This study investigated which adhesins of Pseudomonas aeruginosa interact with the glycolipid asialo GM1, using solid-phase binding and thin-layer chromatography assays. Radioiodinated pili and flagella contaminated with lipopolysaccharide (LPS) bound to the glycolipid. When LPS was reduced to acceptable levels in pilus and flagellum samples, only pili specifically bound to the glycolipid. Commercial, radiolabeled LPS as well as whole bacteria of strain ATCC 19660 also bound to asialo GM1. Binding was specific, competitive, and saturable. Organ cultures of whole mouse eyes and scanning electron microscopy techniques were used also, and strain ATCC 19660 was inhibited from corneal binding by exogenous pili or commercial LPS and inhibition was concentration dependent for both. Binding of radiolabeled strain ATCC 19660 bacteria to neutral lipids extracted from bovine corneal epithelial tissue showed that the bacteria bound to a glycolipid which migrated at a position similar to that of an asialo GM1 standard and that the glycolipid stained positively with an antibody specific for asialo GM1. The data provide evidence that pili (reduced LPS) and LPS of P. aeruginosa bind to asialo GM1 glycolipid and that the glycolipid is not restricted to the mouse cornea.
Analysis of Status of Glucose Metabolism in 97 Cases With Acute Myocardial Infarction (2007)

Objective:To study the status of glucose metabolism in patients with acute myocardial infarction (AMI) with no past history of diabetes. Methods: Ninety-seven patients with AMI without past history of diabetes were studied. HbA1c, blood lipids, simplified OGTT (fasting and 2 hours' postprandial blood glucose), simplified IRT(fasting and 2 hours′ postprandial concentration of insulin) were measured on admission. Regular OGTT and IRT were performed at 4th week in the course of the disease and at 3 months after discharge respectively. The clinical characteristics and blood glucose were recorded. Results: Patients were divided into three groups according to the OGTT results 3 months after discharge: normal glucose tolerance (NGT) group (33 cases), impaired glucose tolerance (IGT) group (34 cases) and diabetic mellitus (DM) group (30 cases). The incidence of abnormal glucose metabolism was 68.04%. There were significant differences in FPG, HbA1C,TG levels among groups with different glucose metabolic status on admission. The glucose metabolic status could be converted mutually among NGT, IGT and DM group within three months in patients with AMI, but the OGTT blood glucose value at 60 minute remains steady, and it could be a predictor of glucose metabolism in the future. Conclusions: Abnormal glucose metabolism were commonly seen in patients with AMI with no past history of diabetes. The glucose metabolic status could be interchanged or remain steady among the NGT, IGT and DM group in the patients with AMI within 3 months. Moreover, single blood glucose value (OGTT, PG-60') at 4th week in the course of the disease was an useful predictor for future glucose metabolic status, and its cut-off value was 9.1 mmol/L.
Degradation of acrylamide by immobilized cells of a <i>Pseudomonas</i> sp. and <i>Xanthomonas maltophilia</i> (1993)

Two bacterial isolates capable of utilizing acrylamide as the sole source of carbon and nitrogen were isolated from herbicide-contaminated soil samples and identified as Pseudomonas sp. and Xanthomonas maltophilia. Batch cultures of Pseudomonas sp. and X. maltophilia completely degraded 62.8 mM acrylamide to acrylic acid and ammonia in 24 and 48 h, respectively. Pseudomonas sp. and X. maltophilia, when immobilized in calcium alginate, markedly increased the rate of degradation of acrylamide over batch cultures. Cells of the isolates immobilized in calcium alginate degraded acrylamide to acrylic acid and ammonia in less than 6 h. Initial metabolism of acrylamide by immobilized cells of Pseudomonas sp. followed by inoculation with nonimmobilized cells after 6 h totally removed acrylamide and its metabolites in 72 h. A similar procedure with X. maltophilia resulted in the total metabolism of acrylamide in 96 h. An inducible, intracellular amidase was responsible for the hydrolysis of acrylamide to acrylic acid and ammonia. The specific activity of Pseudomonas sp. amidase was higher than the specific activity of X. maltophilia amidase.
Epileptic networks in focal cortical dysplasia revealed using electroencephalography–functional magnetic resonance imaging (2011)

Comparison of EEG-fMRI with icEEG suggests that EEG-fMRI may provide useful additional information about the SOZ in FCD. Widely distributed discordant regions of IED-related hemodynamic change appear to be associated with a widespread SOZ and poor postsurgical outcome.
Ketenimines (2007)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Sweet and Sour - Oxidative and Carbonyl Stress in Neurological Disorders (2011)

The nervous system is a unique network of different cell types and comprises a variety of proteins, lipids, and carbohydrates that have an important interplay with all major organs in the body. Homeostatic regulation of nervous tissue turnover must be carefully controlled, taking into account interactions of the nervous, endocrine, and immune systems. Clinical conditions affecting the nervous system range from mild cognitive perturbations such as headache, to life-threatening acute courses such as meningitis and glioblastoma, and to chronic neurodegenerative diseases such as multiple sclerosis. One unifying feature in normal developmental or homeostatic functions and clinical dysfunctions within the nervous system is redox regulation, with an imbalance in oxidative/carbonyl stress versus antioxidants being characteristic of pathological conditions. In this review we consider the state of current knowledge regarding structural, genetic, proteomic, histopathological, clinical, and therapeutic perspectives of oxidative and carbonyl stress within the nervous system.
Graphene-enveloped sulfur in a one pot reaction: a cathode with good coulombic efficiency and high practical sulfur content (2011)

Graphene-sulfur composites with sulfur fractions as high as 87 wt% are prepared using a simple one-pot, scalable method. The graphene envelops the sulfur particles, providing a conductive shrink-wrap for electron transport. These materials are efficient cathodes for Li-S batteries, yielding 93% coulombic efficiency over 50 cycles with good capacity.
Melissa officinalis Protects against Doxorubicin-Induced Cardiotoxicity in Rats and Potentiates Its Anticancer Activity on MCF-7 Cells (2016)

Cardiotoxicity is a limiting factor of doxorubicin (DOX)-based anticancer therapy. Due to its beneficial effects, we investigated whether standardized extract of Melissa officinalis (MO) can attenuate doxorubicin-induced cardiotoxicity and can potentiate the efficacy of DOX against human breast cancer cells. MO was administered orally to male albino rats once daily for 10 consecutive days at doses of 250, 500 and 750 mg/kg b.wt. DOX (15 mg/kg b.wt. i.p.) was administered on the 8th day. MO protected against DOX-induced leakage of cardiac enzymes and histopathological changes. MO ameliorated DOX-induced oxidative stress as evidenced by decreasing lipid peroxidation, protein oxidation and total oxidant capacity depletion and by increasing antioxidant capacity. Additionally, MO pretreatment inhibited inflammatory responses to DOX by decreasing the expressions of nuclear factor kappa-B, tumor necrosis factor-alpha and cyclooxygenase-2 and the activity of myeloperoxidase. MO ameliorated DOX-induced apoptotic tissue damage in heart of rats. In vitro study showed that MO augmented the anticancer efficacy of DOX in human breast cancer cells (MCF-7) and potentiated oxidative damage and apoptosis. Thus, combination of DOX and MO may prove future cancer treatment protocols safer and more efficient.
Diagnosis of bacterial vaginosis: need for validation of microscopic image area used for scoring bacterial morphotypes (2004)

Because of the considerable differences among image areas covered by different microscope set-ups used in Nugent and Ison/Hay scoring, there is a need to standardise the area in order to reach comparable scores reflecting the diagnosis of BV in different laboratories. The differences in the intermediate group will have a considerable effect on the results from both treatment and prevalence studies, even though the kappa indexes indicate very good agreement between the methods used.
Pulsating Fluid Flow Stimulates Prostaglandin Release and Inducible Prostaglandin G/H Synthase mRNA Expression in Primary Mouse Bone Cells (1997)

Bone tissue responds to mechanical stress with adaptive changes in mass and structure. Mechanical stress produces flow of fluid in the osteocyte lacunar-canalicular network, which is likely the physiological signal for bone cell adaptive responses. We examined the effects of 1 h pulsating fluid flow (PFF; 0.7 +/- 0.02 Pa, 5 Hz) on prostaglandin (PG) E2, PGI2, and PGF2alpha production and on the expression of the constitutive and inducible prostaglandin G/H synthases, PGHS-1, and PGHS-2, the major enzymes in the conversion of arachidonic acid to prostaglandins, using mouse calvarial bone cell cultures. PFF treatment stimulated the release of all three prostaglandins under 2% serum conditions, but with a different time course and to a different extent. PGF2alpha was rapidly increased 5-10 minutes after the onset of PFF. PGE2 release increased somewhat more slowly (significant after 10 minutes), but continued throughout 60 minutes of treatment. The response of PGI2 was the slowest, and only significant after 30 and 60 minutes of treatment. In addition, PFF induced the expression of PGHS-2 but not PGHS-1. One hour of PFF treatment increased PGHS-2 mRNA expression about 2-fold relative to the induction by 2% fresh serum given at the start of PFF. When the addition of fresh serum was reduced to 0.1%, the induction of PGHS-2 was 8- to 9-fold in PFF-treated cells relative to controls. This up-regulation continued for at least 1 h after PFF removal. PFF also markedly increased PGHS activity, measured as the conversion of arachidonic acid into PGE2. One hour after PFF removal, the production of all three prostaglandins was still enhanced. These results suggest that prostaglandins are important early mediators of the response of bone cells to mechanical stress. Prostaglandin up-regulation is associated with an induction of PGHS-2 enzyme mRNA, which may subsequently provide a means for amplifying the cellular response to mechanical stress.
Hexameric Calgranulin C (S100A12) Binds to the Receptor for Advanced Glycated End Products (RAGE) Using Symmetric Hydrophobic Target-binding Patches (2006)

Calgranulin C (S100A12) is a member of the S100 family of proteins that undergoes a conformational change upon calcium binding allowing them to interact with target molecules and initiate biological responses; one such target is the receptor for advanced glycation products (RAGE). The RAGE-calgranulin C interaction mediates a pro-inflammatory response to cellular stress and can contribute to the pathogenesis of inflammatory lesions. The soluble extracellular part of RAGE (sRAGE) was shown to decrease the inflammation response possibly by scavenging RAGE-activating ligands. Here, by using high resolution NMR spectroscopy, we identified the sRAGE-calgranulin C interaction surface. Ca2+ binding creates two symmetric hydrophobic surfaces on Ca2+-calgranulin C that allow calgranulin C to bind to the C-type immunoglobulin domain of RAGE. Apo-calgranulin C also binds to sRAGE using a completely different surface and with substantially lower affinity, thus underscoring the role of Ca2+ binding to S100 proteins as a molecular switch. By using native gel electrophoresis, chromatography, and fluorescence spectroscopy, we established that sRAGE forms tetramers that bind to hexamers of Ca2+-calgranulin C. This arrangement creates a large platform for effectively transmitting RAGE-dependent signals from extracellular S100 proteins to the cytoplasmic signaling complexes.
Conformational sensitivity of .beta.-93 cysteine sulfhydryl to ligation of hemoglobin observed by FT-IR spectroscopy (1987)

The SH vibrational absorption of cysteine F9(beta-93) in concentrated aqueous solutions of native liganded hemoglobin (human HbA, horse, and bovine) has been observed by use of Fourier transform infrared spectroscopy. The pattern of beta-93 SH absorption intensity is ligand dependent. In bovine hemoglobin derivatives the SH absorption intensity pattern is (carbonmonoxy)hemoglobin (HbCO) greater than oxyhemoglobin (HbO2) = cyanomethemoglobin (HbCN) much greater than aquomethemoglobin (metHb) and deoxyhemoglobin (deoxyHb). In horse and human hemoglobin derivatives the pattern is HbCO greater than or equal to HbO2 greater than HbCN greater than metHb. The bovine metHb beta-93 SH shows a much lower absorptivity than that of horse or human metHb, and thus it has a different local tertiary equilibrium conformation than does horse or human hemoglobin. X-ray diffraction studies have shown the beta-93 SH in carbon monoxide or oxygen bound hemoglobin to be situated within a nonpolar pocket between the F, G, and H helices. The higher than usual SH absorption frequency (2592 cm-1) that we observe implies there is no hydrogen bonding for this thiol group while situated within this nonpolar pocket. A similar beta-93 SH absorption has been observed in the beta-chain tetramer (thalassemic hemoglobin H in vivo). The beta-112 SH stretching band, previously observed in the alpha 2 beta 2 tetramer, was observed for the first time in the beta-chain tetramer. A band at 2610 cm-1 that is not due to SH was resolved and found to be ligand dependent.
Liquid chromatography/electrospray ionization mass spectrometry method for the quantification of valproic acid in human plasma (2005)

A simple, sensitive and rapid liquid chromatography/electrospray ionization mass spectrometry (LC/ESI-MS) method was developed and validated for the quantification of valproic acid, an antiepileptic drug, in human plasma using benzoic acid as internal standard (IS). Following solid-phase extraction, the analytes were separated using an isocratic mobile phase on a reversed-phase C18 column and analyzed by MS in the single ion monitoring mode using the respective [M-H]- ions, m/z 143 for valproic acid and m/z 121 for the IS. The assay exhibited a linear dynamic range of 0.5-60 microg/mL for valproic acid in human plasma. The lower limit of quantification was 500 ng/mL with a relative standard deviation of less than 10%. Acceptable precision and accuracy were obtained for concentrations over the standard curve range. The average absolute recoveries of valproic acid and the IS from spiked plasma samples were 96.1+/-4.2 and 95.6+/-2.7%, respectively. A run time of 4.5 min for each sample made it possible to analyze more than 250 human plasma samples per day. The validated method has been successfully used to analyze human plasma samples for application in pharmacokinetic, bioavailability and bioequivalence studies.
Fault-Tolerant Operation of a Quantum Error-Correction Code (2020)

Quantum error correction protects fragile quantum information by encoding it into a larger quantum system. These extra degrees of freedom enable the detection and correction of errors, but also increase the operational complexity of the encoded logical qubit. Fault-tolerant circuits contain the spread of errors while operating the logical qubit, and are essential for realizing error suppression in practice. While fault-tolerant design works in principle, it has not previously been demonstrated in an error-corrected physical system with native noise characteristics. In this work, we experimentally demonstrate fault-tolerant preparation, measurement, rotation, and stabilizer measurement of a Bacon-Shor logical qubit using 13 trapped ion qubits. When we compare these fault-tolerant protocols to non-fault tolerant protocols, we see significant reductions in the error rates of the logical primitives in the presence of noise. The result of fault-tolerant design is an average state preparation and measurement error of 0.6% and a Clifford gate error of 0.3% after error correction. Additionally, we prepare magic states with fidelities exceeding the distillation threshold, demonstrating all of the key single-qubit ingredients required for universal fault-tolerant operation. These results demonstrate that fault-tolerant circuits enable highly accurate logical primitives in current quantum systems. With improved two-qubit gates and the use of intermediate measurements, a stabilized logical qubit can be achieved.
Caracteristicas clinicas del lupus eritematoso sistemico (les). Informe de 22 casos (1982)

Se presenta el estudio de 22 pacientes a quienes se les diagnostico LES en el periodo comprendido de 1973 a 1978 en el hospital provincial docente Manuel Ascunce Domenech, de Camaguey. El LES fue predominante en pacientes dpi sexo femenino, jovenes y de la raza blanca. Las manifestaciones clinicas fueron multiples y variadas. El vespertilio y la alopecia fueron las principales alteraciones dermatologicas; mientras que la disnea y las neumopatias, asi como los signos de insuficiencia cardiaca y de hipertension arterial fueron las principales alteraciones respiratorias y cardiovasculares. La dispepsia, constipacion y el sindrome ulceroso, predominaron entre las afecciones digestivas. La anemia represento el 95,5% entre las afecciones hematicas. La urosepsis, la disuria y la polaquiuria se encontraron en nuestra serie como las principales manifestaciones genitourinarias. Los medicamentos empleados con mayor frecuencia fueron los antibioticos, los esteroides y los antipaludicos; y con menor cuantia los inmunosupresores. Se revisa la literatura medica al respecto, y se comparan nuestros resultados con los de otros autores.
A New Software Demodulation Method For MSK Signal Based On Phase Information (2006)

The evolution of digital modulation signals is reviewed in this article firstly, including PSK, FSK, QPSK, OQPSK, MSK etc. Secondly the modulation and demodulation of MSK are taken as a focal point and a new efficient demodulation method based on instantaneous phase information is put forward. Thirdly simulation validates the new method
Use of near infrared spectroscopy and multivariate analysis to predict the wood density of Douglas-fir from chain saw chips (2006)

In many parts of the world, log markets are becoming increasingly competitive and complex. Wood properties, such as stiffness, density, spiral grain, and extractives content, are now being considered by log buyers. Real-time assessment of these properties will be a challenge for log supply managers. The utility of near infrared (NIR) technology for predicting wood density in Douglas-fir stems was examined. Wood disks were collected from 17 sites around Oregon. Each disk was cut with a chain saw, of similar gauge to that used on mechanized harvesters/processors, to provide saw chips. NIR spectra were then obtained for the chip samples. Multivariate techniques were used to correlate wood properties with the NIR spectra. Preliminary research results showed that NIR could be used to predict density. Coefficients of determination ranged between 0.89 and 0.95 for calibration models and between 0.56 and 0.85 for validation models. These results indicate that NIR technology could be used by mechanized harvesting equipment for log segregation based on wood density. Forest Products Society 2006.
The Many Faces of Glutathione in Bacteria (2006)

Glutathione is one of the most abundant thiols present in cyanobacteria and proteobacteria, and in all mitochondria or chloroplast-bearing eukaryotes. In bacteria, in addition to its key role in maintaining the proper oxidation state of protein thiols, glutathione also serves a key function in protecting the cell from the action of low pH, chlorine compounds, and oxidative and osmotic stresses. Moreover, glutathione has emerged as a posttranslational regulator of protein function under conditions of oxidative stress, by the direct modification of proteins via glutathionylation. This review summarizes the biosynthesis and function of glutathione in bacteria from physiological and biotechnological standpoints.
Full-Scale Characterization of UVLED Al<sub><i>x</i></sub>Ga<sub>1–<i>x</i></sub>N Nanowires <i>via</i> Advanced Electron Microscopy (2013)

III-Nitride semiconductor heterostructures continue to attract a great deal of attention due to the wide range of wavelengths at which they can emit light, and the subsequent desire to employ them in optoelectronic applications. Recently, a new type of pn-junction which relies on polarization-induced doping has shown promise for use as an ultraviolet light emitting diode (UVLED); nanowire growth of this device has been successfully demonstrated. However, as these devices are still in their infancy, in order to more fully understand their physical and electronic properties, they require a multitude of characterization techniques. Specifically, the present contribution will discuss the application of advanced scanning transmission electron microscopy (STEM) to AlxGa1-xN UVLED nanowires. In addition to structural data, chemical and electronic properties will also be probed through various spectroscopy techniques, with the focus remaining on practically applying the knowledge gained via STEM to the growth procedures in order to optimize device peformance.
Comprehensive risk reduction in patients with atrial fibrillation: emerging diagnostic and therapeutic options--a report from the 3rd Atrial Fibrillation Competence NETwork/European Heart Rhythm Association consensus conference (2011)

While management of atrial fibrillation (AF) patients is improved by guideline-conform application of anticoagulant therapy, rate control, rhythm control, and therapy of accompanying heart disease, the morbidity and mortality associated with AF remain unacceptably high. This paper describes the proceedings of the 3rd Atrial Fibrillation NETwork (AFNET)/European Heart Rhythm Association (EHRA) consensus conference that convened over 60 scientists and representatives from industry to jointly discuss emerging therapeutic and diagnostic improvements to achieve better management of AF patients. The paper covers four chapters: (i) risk factors and risk markers for AF; (ii) pathophysiological classification of AF; (iii) relevance of monitored AF duration for AF-related outcomes; and (iv) perspectives and needs for implementing better antithrombotic therapy. Relevant published literature for each section is covered, and suggestions for the improvement of management in each area are put forward. Combined, the propositions formulate a perspective to implement comprehensive management in AF.