gebhart/patrepeval huggingface dataset:
Single Delivery of an Adeno-Associated Viral Construct to Transfer the <i>CASQ2</i> Gene to Knock-In Mice Affected by Catecholaminergic Polymorphic Ventricular Tachycardia Is Able to Cure the Disease From Birth to Advanced Age (2014)

Our data demonstrate that viral gene transfer of wild-type CASQ2 into the heart of R33Q mice prevents and reverts severe manifestations of catecholaminergic polymorphic ventricular tachycardia and that this curative effect lasts for 1 year after a single injection of the vector, thus posing the rationale for the design of a clinical trial.
Growth Hormone–Releasing Hormone Agonists Reduce Myocardial Infarct Scar in Swine With Subacute Ischemic Cardiomyopathy (2015)

Daily subcutaneous administration of GHRH-A is feasible and safe in a large animal model of subacute ischemic cardiomyopathy. Furthermore, GHRH-A therapy significantly reduced infarct size and improved diastolic strain, suggesting a local activation of the GHRH pathway leading to the reparative process.
Biocatalytic Yarn for Peroxide Decomposition with Controlled Liquid Transport (2021)

Abstract A robust biocatalytic yarn with controllable liquid transport properties is created by coating thin layers of chitosan containing catalase onto a cellulosic yarn. The resulting material integrates enzyme catalytic functionality with protective coating properties of chitosan and structural functionality of the textile. Mild immobilization conditions and good affinity between the two polysaccharides minimize enzyme inactivation during the preparation steps and prevent enzyme from leaching during peroxide decomposition testing and washing, providing a novel and versatile enzyme immobilization strategy. The catalytic efficiency of enzymes in a reaction containing solid, liquid, and gas phases is facilitated when dissolved enzyme substrate is transported by liquid flowing through the coated textile structure. The flow‐through configuration decomposes at least two times more peroxide in a twenty‐times smaller reaction zone volume compared to a stirred tank configuration. Liquid transport through the yarn and liquid spatial distribution within the yarn are investigated by in situ neutron radiography and neutron computed tomography, revealing a constrained wicking mechanism that benefits biocatalytic yarn performance. This new class of sustainable and flexible biocatalytic textile matrices has beneficial multifunctional properties, not previously described, that are applicable for numerous small‐ and large‐scale applications including controlled flow reactors and reactive filtration.
Adaptive hyperspectral imaging with a MEMS-based full-frame programmable spectral filter (2014)

Rapidly programmable spatial light modulation devices based on MEMS technology have opened an exciting new arena in spectral imaging: rapidly reprogrammable, high spectral resolution, multi-band spectral filters that enable hyperspectral processing directly in the optical hardware of an imaging sensor. Implemented as a multiplexing spectral selector, a digital micro-mirror device (DMD) can independently choose or reject dozens or hundreds of spectral bands and present them simultaneously to an imaging sensor, forming a complete 2D image. The result is a high-speed, highresolution, programmable spectral filter that gives the user complete control over the spectral content of the image formed at the sensor. This technology enables a wide variety of rapidly reprogrammable operational capabilities within the same sensor including broadband, color, false color, multispectral, hyperspectral and target specific, matched filter imaging. Of particular interest is the ability to implement target-specific hyperspectral matched filters directly into the optical train of the sensor, producing an image highlighting a target within a spectrally cluttered scene in real time without further processing. By performing the hyperspectral image processing at the sensor, such a system can operate with high performance, greatly reduced data volume, and at a fraction of the cost of traditional push broom hyperspectral instruments. Examples of color, false color and target-specific matched-filter images recorded with our visible-spectrum prototype will be displayed, and extensions to other spectral regions will be discussed.
Effect of deep insertion of the cochlear implant electrode array on pitch estimation and speech perception (2006)

The results showed that activating the electrodes in the uppermost region of the cochlea improves speech perception significantly. Furthermore it could be demonstrated that the pitch perceived in the cochlea with electrical stimulation decreases with increasing insertion depth along the whole length of deeply inserted electrode arrays.
Rapid mapping of digital integrated circuit logic gates via\n multi-spectral backside imaging (2016)

Modern semiconductor integrated circuits are increasingly fabricated at\nuntrusted third party foundries. There now exist myriad security threats of\nmalicious tampering at the hardware level and hence a clear and pressing need\nfor new tools that enable rapid, robust and low-cost validation of circuit\nlayouts. Optical backside imaging offers an attractive platform, but its\nlimited resolution and throughput cannot cope with the nanoscale sizes of\nmodern circuitry and the need to image over a large area. We propose and\ndemonstrate a multi-spectral imaging approach to overcome these obstacles by\nidentifying key circuit elements on the basis of their spectral response. This\nobviates the need to directly image the nanoscale components that define them,\nthereby relaxing resolution and spatial sampling requirements by 1 and 2 - 4\norders of magnitude respectively. Our results directly address critical\nsecurity needs in the integrated circuit supply chain and highlight the\npotential of spectroscopic techniques to address fundamental resolution\nobstacles caused by the need to image ever shrinking feature sizes in\nsemiconductor integrated circuits.\n
A randomized, subject and rater-blinded, placebo-controlled trial of dimethyl fumarate for obstructive sleep apnea (2018)

ClinicalTrials.gov, NCT02438137, https://clinicaltrials.gov/ct2/show/NCT02438137?term=NCT02438137&rank=1.
Motion-constrained AV1 Encoder for 360 VR Tiled Streaming (2018)

The high dynamic range, wide-angle, and high-resolution technologies provide the more advanced experiences, such as 360 virtual reality (VR) streaming content. However, increasing streaming content challenges the traditional delivery systems, requiring higher bitrates and more efficient video codecs. This paper presents the motion-constrained AV1 encoder for a 360 VR streaming application. We implement the AV1 encoder with restricted motion range and AV1 encoding features, which is not compatible with tiled streaming.
High-repetition-rate CF_4 laser (1982)

A 16-microm CF(4) laser oscillator has operated at 1 kHz in a cooled static cell. Pump energies required from the lowpressure, Q-switched, cw-discharge CO(2) laser were as low as 60 microJ. The laser cavity was a multiple-pass off-axispath two-mirror resonator in a ring configuration. CF(4) laser power at 615 cm(-1) and a 1-kHz repetition rate exceeded 300 microW.
A Genetically Encoded Multifunctional TRAIL Trimer Facilitates Cell-Specific Targeting and Tumor Cell Killing (2010)

Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL, Apo2L) has been shown to exhibit potent and specific apoptotic activity against tumor cells. Several TRAIL constructs have been tried in patients, and the molecule remains under active clinical investigation. Native and recombinant TRAIL must form a homotrimer to become biologically active. However, noncovalently associated TRAIL displays a high degree of sensitivity to degradation, which limits its therapeutic potential. To enforce trimerization of the recombinant protein, we developed a covalently linked TRAIL trimer (TR3) by genetic fusion. This molecular drug design conferred improved stability without altering the native killing ability of TRAIL. Target specificity was shown by blocking TR3 activity with soluble death receptor 5 (DR5-Fc). In addition, we have shown that TR3 is amenable to further, genetic modifications. The incorporation of additional functional domains to TR3, such as antibody fragments (scFvs) that allow for a more cell-specific delivery of the agent, is stoichiometrically controlled and inconsequential with regard to the bioactivity of TRAIL. As proof of this concept, TR3 activity was targeted to the mouse RBC membrane. TR3-decorated RBCs were effectively capable of target cell killing in a model of pancreatic cancer. TR3 represents a generally applicable platform tool to study basic mechanisms along the death receptor pathway. More importantly, the ability to target TR3 to a cell surface presents the opportunity to create a cancer-selective drug with fewer off-target toxicities and enhanced killing capacities.
A channel-selection criterion for suppressing reverberation in cochlear implants (2011)

Little is known about the extent to which reverberation affects speech intelligibility by cochlear implant (CI) listeners. Experiment 1 assessed CI users' performance using Institute of Electrical and Electronics Engineers (IEEE) sentences corrupted with varying degrees of reverberation. Reverberation times of 0.30, 0.60, 0.80, and 1.0 s were used. Results indicated that for all subjects tested, speech intelligibility decreased exponentially with an increase in reverberation time. A decaying-exponential model provided an excellent fit to the data. Experiment 2 evaluated (offline) a speech coding strategy for reverberation suppression using a channel-selection criterion based on the signal-to-reverberant ratio (SRR) of individual frequency channels. The SRR reflects implicitly the ratio of the energies of the signal originating from the early (and direct) reflections and the signal originating from the late reflections. Channels with SRR larger than a preset threshold were selected, while channels with SRR smaller than the threshold were zeroed out. Results in a highly reverberant scenario indicated that the proposed strategy led to substantial gains (over 60 percentage points) in speech intelligibility over the subjects' daily strategy. Further analysis indicated that the proposed channel-selection criterion reduces the temporal envelope smearing effects introduced by reverberation and also diminishes the self-masking effects responsible for flattened formants.
A new scenario of triple-hop mixed RF/FSO/RF relay network with generalized order user scheduling and power allocation (2016)

This paper proposes and evaluates the performance of multiuser (MU) triple-hop mixed radio frequency (RF)/free-space optical (FSO) relay network with generalized order user scheduling. An important example on the applicability of this scenario is in cellular networks where two sets of various users are communicating with their own base stations (BSs) over RF links and these BSs are connected together via an FSO link. The considered system includes K 1 sources or users, two decode-and-forward (DF) relays, and K 2 destinations or users. The sources and destinations are connected with their relay nodes through RF links, and the relays are connected with each other through an FSO link. To achieve MU diversity, the generalized order user scheduling is used on the RF hops to select among sources and destinations. In the analysis, the RF channels are assumed to follow the Rayleigh fading model and the FSO channel is assumed to follow the Gamma-Gamma fading model including the effect of pointing errors. Closed-form expressions are derived for the outage probability, average symbol error probability (ASEP), and ergodic channel capacity. Moreover, in order to gain more insight onto the system behavior, the system is studied at the signal-to-noise ratio (SNR) regime whereby the diversity order and coding gain are provided and studied. The asymptotic results are used to obtain the optimum transmission power of the system. Monte Carlo simulations are given to validate the achieved exact and asymptotic results. The results show that the diversity order and coding of the proposed scenario are determined by the worst link among the three links. Also, results illustrate the effectiveness of the proposed power allocation algorithm in enhancing the system performance compared to the case with no power allocation.
Detection by Reverse Transcription-PCR and Genetic Characterization of Field Isolates of Swine Hepatitis E Virus from Pigs in Different Geographic Regions of the United States (2002)

Hepatitis E virus (HEV) is an important public health concern in many developing countries. HEV is also endemic in some industrialized counties, including the United States. With our recent discovery of swine HEV in pigs that is genetically closely related to human HEV, hepatitis E is now considered a zoonotic disease. Human strains of HEV are genetically heterogenic. So far in the United States, only one strain of swine HEV has been identified and characterized from a pig. To determine the extent of genetic variations and the nature of swine HEV infections in U.S. pigs, we developed a universal reverse transcription-PCR (RT-PCR) assay that is capable of detecting genetically divergent strains of HEV. By using this universal RT-PCR assay, we tested fecal and serum samples of pigs of 2 to 4 months of age from 37 different U.S. swine farms for the presence of swine HEV RNA. Thirty-four of the 96 pigs (35%) and 20 of the 37 swine herds (54%) tested were positive for swine HEV RNA. The sequences of a 348-bp region within the ORF2 gene of 27 swine HEV isolates from different geographic regions were determined. Sequence analyses revealed that the 27 U.S. swine HEV isolates shared 88 to 100% nucleotide sequence identities with each other and 89 to 98% identities with the prototype U.S. strain of swine HEV. These U.S. swine HEV isolates are only distantly related to the Taiwanese strains of swine HEV, with about 74 to 78% nucleotide sequence identities; to most known human strains of HEV worldwide, with <79% sequence identities; and to avian HEV, with 54 to 56% sequence identities. Phylogenetic analysis showed that all the U.S. swine HEV isolates identified in this study clustered in the same genotype with the prototype U.S. swine HEV and the two U.S. strains of human HEV. The data from this study indicated that swine HEV is widespread and enzoonotic in U.S. swine herds and that, as is with human HEV, swine HEV isolates from different geographic regions of the world are also genetically heterogenic. These data further raise potential concerns for zoonosis, xenozoonosis, and food safety.
Human monoclonal antibodies to group B streptococcus. Reactivity and in vivo protection against multiple serotypes. (1988)

Group B streptococcal (GBS) infections cause significant mortality and morbidity among infants. Passive antibody immunotherapy has been proposed as treatment for infected infants. To this end, two human mAb-secreting cell lines were produced by EBV immortalization of human B cells. The mAbs were specific for the group B polysaccharide and bound to strains of all five serotypes as demonstrated by ELISA and crossed immunoelectrophoresis. The mAbs reacted and opsonized 100% (132/132) of the clinical isolates tested which represented all four capsule types. Both prophylactic and therapeutic protection with these mAbs were demonstrated in neonatal rats given lethal infections of types Ia and III human clinical isolates. These data indicate that a single human mAb directed against the group B carbohydrate can protect against GBS infections caused by the different serotypes. This antibody may be useful in the passive immunotherapy of infants infected with GBS.
Design and Synthesis of Highly Potent Benzodiazepine γ-Secretase Inhibitors:  Preparation of (2<i>S</i>,3<i>R</i>)-3-(3,4- Difluorophenyl)-2-(4-fluorophenyl)-4- hydroxy-<i>N</i>-((3<i>S</i>)-1-methyl-2-oxo-5- phenyl-2,3-dihydro-1<i>H</i>-benzo[<i>e</i>][1,4]- diazepin-3-yl)butyramide by Use of an Asymmetric Ireland−Claisen Rearrangement (2003)

Novel benzodiazepine-containing gamma-secretase inhibitors for potential use in Alzheimer's disease have been designed that incorporate a substituted hydrocinnamide C-3 side chain. A syn combination of alpha-alkyl or aryl and beta-hydroxy or hydroxymethyl substituents was shown to give highly potent compounds. In particular, (2S,3R)-3-(3,4-difluorophenyl)-2-(4-fluorophenyl)-4-hydroxy-N-((3S)-2-oxo-5-phenyl-2,3-dihydro-1H-benzo[e][1,4]diazepin-3-yl)butyramide (34) demonstrated excellent in vitro potency (IC(50) = 0.06 nM). 34 could also be selectively methylated to give [(3)H]-28, which is of use in radioligand binding assays.
Highly efficient fluorescence detection in picoliter volume liquid-core waveguides (2005)

We report loss improvement and fluorescence detection in integrated antiresonant reflecting optical waveguides with liquid cores. The minimum waveguide loss is reduced to 0.33∕cm by compensating for thickness variations in the fabrication process. We demonstrate fluorescence detection from as few as 490molecules in a 57pl core using these optimized waveguides. We measure angular fluorescence collection factors as high as 15% per facet in good agreement with theory. This demonstrates the potential of integrated hollow-core waveguides as optical sensors for single-molecule spectroscopy.
Modulation of Regulatory T Cell Function by Monocyte-Derived Dendritic Cells Matured through Electroporation with mRNA Encoding CD40 Ligand, Constitutively Active TLR4, and CD70 (2013)

Regulatory T cells (Tregs) counteract anticancer immune responses through a number of mechanisms, limiting dendritic cell (DC)-based anticancer immunotherapy. In this study, we investigated the influence of various DC activation stimuli on the Treg functionality. We compared DCs activated by electroporation with mRNA encoding constitutively active TLR4 (caTLR4) and CD40 ligand (DiMix-DCs), or these factors together with mRNA encoding the costimulatory molecule CD70 (TriMix-DCs) with DCs maturated in the presence of a mixture of inflammatory cytokines (DCs maturated with a combination of the cytokines IL-1β, IL-6, TNF-α, and PGE2) for their ability to counteract Tregs on different levels. We first demonstrated that there was no difference in the extent of Treg induction starting from CD4(+)CD25(-) T cells under the influence of the different DC maturation stimuli. Second, we showed that both DiMix- and TriMix-DCs could partly alleviate Treg inhibition of CD8(+) T cells. Third, we observed that CD8(+) T cells that had been precultured with DiMix-DCs or TriMix-DCs were partially protected against subsequent Treg suppression. Finally, we showed that Tregs cocultured in the presence of TriMix-DCs, but not DiMix-DCs, partially lost their suppressive capacity. This was accompanied by a decrease in CD27 and CD25 expression on Tregs, as well as an increase in the expression of T-bet and secretion of IFN-γ, TNF-α, and IL-10, suggesting a shift of the Treg phenotype toward a Th1 phenotype. In conclusion, these data suggest that TriMix-DCs are not only able to suppress Treg functions, but moreover could be able to reprogram Tregs to Th1 cells under certain circumstances.
Accuracy of Immunodiagnostic Tests for Active Tuberculosis Using Single and Combined Results: A Multicenter TBNET-Study (2008)

The assay based on RD1 selected peptides has similar accuracy for active tuberculosis compared with TST and commercial IGRAs. Then, independently of the spectrum of antigens used in the assays to elicit mycobacterial specific immune responses, the novel test, IGRAs, and the TST do not allow an accurate identification of active tuberculosis in clinical practice. However, the combined use of the novel assay or commercial IGRAs with TST may allow exclusion of tuberculosis.
A multiconductor transmission line methodology for global on-chip interconnect modeling and analysis (2004)

This paper describes a methodology for global on-chip interconnect modeling and analysis using frequency-dependent multiconductor transmission lines. The methodology allows designers to contain the complexity of series impedance computation by transforming the generic inductance and resistance extraction problem into one of per-unit-length parameter extraction. This methodology has been embodied in a CAD tool that is now in production use by interconnect designers and complementary metal oxide semiconductor (CMOS) process technologists.
A practical synthesis of 5-(4′-methylbiphenyl-2-yl)-1<i>H</i>-tetrazole (2006)

Practical synthesis of 5-(4′-methylbiphenyl-2-yl)-1H-tetrazole, key intermediate in several angiotensin II receptor antagonists from 2-fluorobenzonitrile in excellent yields and very high purity is described.
Noninvasive Markers of Airway Inflammation in Asthma (2009)

Findings from this pilot study indicate that urinary levels of bromotyrosine and F(2)-isoprostanes, in addition to exhaled nitric oxide levels, are associated with asthma.
Eudistomins C, E, K, and L, potent antiviral compounds containing a novel oxathiazepine ring from the Caribbean tunicate Eudistoma olivaceum (1984)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEudistomins C, E, K, and L, potent antiviral compounds containing a novel oxathiazepine ring from the Caribbean tunicate Eudistoma olivaceumKenneth L. Rinehart, Junichi Kobayashi, Gary C. Harbour, Robert G Hughes Jr., Stephen A. Mizsak, and Terrence A. ScahillCite this: J. Am. Chem. Soc. 1984, 106, 5, 1524–1526Publication Date (Print):March 1, 1984Publication History Published online1 May 2002Published inissue 1 March 1984https://pubs.acs.org/doi/10.1021/ja00317a079https://doi.org/10.1021/ja00317a079research-articleACS PublicationsRequest reuse permissionsArticle Views482Altmetric-Citations167LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Time-Aware Multi-Application Task Scheduling With Guaranteed Delay Constraints in Green Data Center (2017)

A growing number of companies deploy their applications in green data centers (GDCs) and provide services to tasks of global users. Currently, a growing number of GDC providers aim to maximize their profit by deploying green energy facilities and decreasing brown energy consumption. However, the temporal variation in the revenue, price of grid, and green energy in tasks' delay bounds makes it challenging for GDC providers to achieve profit maximization while strictly guaranteeing delay constraints of all admitted tasks. Unlike existing studies, a timeaware task scheduling (TATS) algorithm that investigates the temporal variation and schedules all admitted tasks to execute in GDC meeting their delay bounds is proposed. In addition, this paper provides the mathematical modeling of task refusal and service rates. In each iteration, TATS solves the formulated profit maximization problem by hybrid chaotic particle swarm optimization based on simulated annealing. Compared with several existing scheduling algorithms, TATS can increase profit and throughput without violating delay constraints of all admitted tasks.