gebhart/patrepeval huggingface dataset:
Abstract A058: Selective targeting of T regulatory cells by a TCR-mimic monoclonal antibody specific for foxp3-derived epitopes (2016)

Abstract Immunosuppression by tumor-induced regulatory T (Treg) cells present major obstacles for successful immunotherapy. Treg expansion and its negative prognostic impact represent a general phenomenon seen in multiple types of cancer. Therefore, developing strategies for Treg depletion could enhance the reactivation of immune responses against the malignant tumor cells. However, the strategies of depleting Tregs have been hindered by the lack of specificity, which also leads to the depletion of anti-tumor effector cells. The transcription factor forkhead box p3 (FoxP3) is selectively expressed in and is essential to the suppressive function of Treg cells. FoxP3 thus would be an appealing target for eliminating Treg cells. However, Foxp3 is an undruggable intracellular protein. Here, we took a novel and unconventional approach to target Foxp3 by using a T cell receptor mimic (TCRm) mAb, which recognizes a human Foxp3-derived CD8 T cell epitope, presented by HLA-A*02:01 molecule. We show that the Foxp3 mAb specifically bound to CD4+CD25hiCD127lo Foxp3+ Treg cells from HLA-A0201 positive donors, and tumor cell lines co-expressing Foxp3 and HLA-A*02;01 molecule. Both afucosylated Fc enhanced human IgG1 and bispecific T cell engager formats of the Foxp3 mAb are able to kill in vitro-generated Treg clones from HLA-A0201+ donors and “Treg-like” cutaneous lymphoma cells (HLA-A*02:01+) that have a high level expression of CD4, CD25 and Fox3+. FoxP3-targeting antibodies could potentially be a novel approach in cancer immunotherapy by overcoming immunosuppression caused by Tregs and tumor cells expressing Foxp3. Citation Format: Tao Dao, Casey Jarvis, Andrew C. Scott, Tatyana Korontsvit, Victoria Zakhaleva, Dmitry Pankov, Manuel Direito de Morais Guerrerio, Melissa Mathias, Neal Cheng, Cheng Liu, David A. Scheinberg. Selective targeting of T regulatory cells by a TCR-mimic monoclonal antibody specific for foxp3-derived epitopes [abstract]. In: Proceedings of the Second CRI-CIMT-EATI-AACR International Cancer Immunotherapy Conference: Translating Science into Survival; 2016 Sept 25-28; New York, NY. Philadelphia (PA): AACR; Cancer Immunol Res 2016;4(11 Suppl):Abstract nr A058.
Helices α2 and α3 of West Nile Virus Capsid Protein Are Dispensable for Assembly of Infectious Virions (2009)

The internal hydrophobic sequence within the flaviviral capsid protein (protein C) plays an important role in the assembly of infectious virions. Here, this sequence was analyzed in a West Nile virus lineage I isolate (crow V76/1). An infectious cDNA clone was constructed and used to introduce deletions into the internal hydrophobic domain which comprises helix alpha2 and part of the loop intervening helices alpha2 and alpha3. In total, nine capsid deletion mutants (4 to 14 amino acids long) were constructed and tested for virus viability. Some of the short deletions did not significantly affect growth in cell culture, whereas larger deletions removing almost the entire hydrophobic region significantly impaired viral growth. Efficient growth of the majority of mutants could, however, be restored by the acquisition of second-site mutations. In most cases, these resuscitating mutations were point mutations within protein C changing individual amino acids into more hydrophobic residues, reminiscent of what had been observed previously for another flavivirus, tick-borne encephalitis virus. However, we also identified viable spontaneous pseudorevertants with more than one-third of the capsid protein removed, i.e., 36 or 37 of a total of 105 residues, including all of helix alpha3 and a hydrophilic segment connecting alpha3 and alpha4. These large deletions are predicted to induce formation of large, predominantly hydrophobic fusion helices which may substitute for the loss of the internal hydrophobic domain, underlining the unrivaled structural and functional flexibility of protein C.
One-step synthesis of graphene via catalyst-free gas-phase hydrocarbon detonation (2013)

A one-step, gas-phase, catalyst-free detonation of hydrocarbon (C2H2) method was developed to produce gram quantities of pristine graphene nanosheets (GNs). The detonation of C2H2 was carried out in the presence of O2. The molar ratios of O2/C2H2 were 0.4, 0.5, 0.6, 0.7 and 0.8. The obtained GNs were analyzed by XRD, TEM, XPS and Raman spectroscopy. The GNs are crystalline with a (002) peak centered at 26.05° (d = 0.341 nm). TEM shows that the GNs are stacked in two to three layers and sometimes single layers. An increase in the size of the GNs (35-250 nm) along with a reduction in defects (Raman I(D)/I(G) ~ 1.33-0.28) and specific surface area (187-23 m(2) g(-1)) was found with increasing O2 content. The high temperature of the detonation, ca. 4000 K, is proposed as the cause of graphene production rather than normal soot. The method allows for the control of the number of layers, shape and size of the graphene nanosheets. The process can be scaled up for industrial production.
Three-phase bidirectional DC-DC converter with enhanced current sharing capability (2010)

Current unbalance is an important issue in multiphase dc-dc converters. In this paper, three types of three-phase bidirectional dc-dc converters are introduced. Comparing to other two types of converters, the Y-connection type converter reduces the current mismatch significantly due to the inherent current sharing capability. To additionally alleviate current mismatch, a small signal method is applied to derive the relationship between phase shift angle and the transformer current. Based on the analysis, a closed loop control strategy and RPC (Ratio Presetting Controller) method are developed to enhance current sharing capability. Simulation and experimental results on a 6 kW prototype are presented to verify the proposed method.
Gate turn-off capability of depletion-mode thyristors (1989)

Experimental results are described which demonstrate the ability to switch a thyristor from its ON state to its OFF state by using a depletion layer formed by the application of gate bias to a trench-gate MOSFET integrated within the thyristor structure. The maximum controllable current is found to be a function of the gate bias voltage, the trench depth, and the ambient temperature. The maximum controllable current can be increased by increasing the trench depth and decreasing the p-base sheet resistance. The maximum controllable current decreases at high temperatures, as in the case of other MOS-bipolar devices, but is significantly better than for previous devices. The absolute values of the maximum turnoff current are well above 1000 A/cm/sup 2/ at room temperature and 500 A/cm/sup 2/ at 200 degrees C.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Molecular Inhibitor of QSOX1 Suppresses Tumor Growth <i>In Vivo</i> (2019)

Quiescin sulfhydryl oxidase 1 (QSOX1) is an enzyme overexpressed by many different tumor types. QSOX1 catalyzes the formation of disulfide bonds in proteins. Because short hairpin knockdowns (KD) of QSOX1 have been shown to suppress tumor growth and invasion <i>in vitro</i> and <i>in vivo</i>, we hypothesized that chemical compounds inhibiting QSOX1 enzymatic activity would also suppress tumor growth, invasion, and metastasis. High throughput screening using a QSOX1-based enzymatic assay revealed multiple potential QSOX1 inhibitors. One of the inhibitors, known as "SBI-183," suppresses tumor cell growth in a Matrigel-based spheroid assay and inhibits invasion in a modified Boyden chamber, but does not affect viability of nonmalignant cells. Oral administration of SBI-183 inhibits tumor growth in 2 independent human xenograft mouse models of renal cell carcinoma. We conclude that SBI-183 warrants further exploration as a useful tool for understanding QSOX1 biology and as a potential novel anticancer agent in tumors that overexpress QSOX1.
A State‐of‐Charge Test for Zinc‐Mercuric Oxide Primary Cells (1975)

A readily instrumented and conducted nondestructive technique to determine the state of charge of zinc‐mercuric oxide primary cells has been developed and preliminary results obtained. Measurements of the current vs.voltage (I‐V) characteristics, the a‐c impedance, the electronically sampled derivative at different sampling currents and frequencies, and the discharge‐time characteristics of the cells as a function of charge expended have been made and reported. The nearly constant slopes of the I‐V curves above 75 mA were measured graphically. It was found that these slopes have a linear relation to charge expended and can be used as a basis for a simple state‐of‐charge indicator which determines remaining charge to about 12%. By combining this criterion with a‐c resistance measurements the maximum experimental deviation was found to be less than 10% with 90% of the data points deviating less than 6% from the mean linear curve. These studies indicate that this technique constitutes a useful, easily applied state‐of‐charge test.
Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry: A High-Throughput Platform for Metabolomics with High Data Fidelity (2013)

A major constraint in large-scale mass spectrometry (MS)-based metabolomic initiatives is the low sample throughput associated with chromatographic or electrophoretic separations. Herein, we introduce multisegment injection-capillary electrophoresis-mass spectrometry (MSI-CE-MS) as a multiplexed separation platform for metabolomics that increases sample throughput up to one order of magnitude while improving overall data fidelity. We demonstrate that serial injection of seven or more discrete sample segments can be performed within a single capillary while maintaining isomeric resolution without ion suppression when using a high mass resolution time-of-flight-MS. Customized injection sequences can be devised to encode information temporally within a separation based on signal pattern recognition, which enables unambiguous identification and accurate quantification (mean bias <10%) of polar metabolites in human plasma with good reproducibility (CV ≈ 10%, n = 70). False discoveries are avoided when using a rigorous dilution trend filter to reject spurious signals and background peaks that comprise the majority (≈65%) of total detectable features. MSI-CE-MS offers an unprecedented approach to enhance sample throughput analogous to direct infusion-MS (≈3 min/sample) while delivering far greater selectivity, quantitative performance, and data quality since the same ion from different samples migrates into the ion source within a short time interval (≈2-6 min). These distinct analytical and bioinformatic merits are achieved without column switching, isotopic labeling, hardware modifications, or costly infrastructure investments.
Generalized multiplicative network coding for the broadcast phase of bidirectional relaying (2011)

A generalization of symbol-level multiplicative network coding is presented in this paper. The effects of non-constant amplitude modulation on the error probability of bidirectional relaying links are investigated analytically and through computer simulations. A constellation rearrangement is then presented to further enhance the performance. Simulation results show that the proposed method is able to alleviate the link asymmetry problem in bidirectional relaying and provide considerable throughput gains compared to equally simple network coding techniques.
Time-reduced and large-area polycrystalline silicon thin-film transistor technology based on metal-induced crystallization (2005)

Techniques of forming polycrystalline silicon (poly-Si) using peripherally crystallized poly-Si (PCP) and solution-based large-area crystallization (LAC) are reported. Though these processes offer advantages of reduced mask-count and reduced/eliminated pattern distortion resulting from glass-shrinkage, the resulting thin-film transistor (TFT) performance is comparatively degraded.
Area–efficient multiport memories for the Tb/s bandwidth era (1999)

Tb/s memory-access bandwidth becomes conceivable, if N>30 ports with parallel access capability are available. Conventional multiport-memory architectures cannot realize such memories efficiently, because memory-cell area explodes due to quadratic area increase with N. 32- port-memory cells e. g. are estimated factors 80 (SRAM, large 1-port cell) and 400 (ROM, small 1-port cell) larger than correspondng 1-port cells. A recently proposed hierarchical architecture [1] can reduce this unacceptable area consumption to a small fraction, because the smallest unit-size with N-port capability is increased by using blocks of 1-port-cells. Possible area reductions become larger with increasing port number and storage capacity. For hierarchical 32-port SRAMs with storage capacities >6Mb enormous reductions to area-fractions <1/20 of the conventional architecture are estimated on the basis of design data. Since high clock frequencies can be achieved with pipeline concepts, Tb/s bandwidth VLSI systems become already a realistic perspective with today's advanced CMOS technologies.
A Search for the Osteogenic Factor in Dentin. Rat Incisor Dentin Contains a Factor Stimulating Rat Muscle Cells in Vitro to Incorporate Sulfate into An Altered Proteoglycan (1989)

Demineralized dentin matrix has the capacity to induce bone formation via a chondrogenic pathway when implanted into muscle, in a fashion entirely analogous to bone matrix implants. In this work we have attempted to isolate, from rat incisor dentin, the matrix factor responsible for initiating osteogenesis. Rat incisor dentin was demineralized with EDTA plus 4.0 M guanidine. HCl. The proteins in the extracts were collected and, after a CaCl2 precipitation step, fractionated on Sephacryl S-200 in 6.0 M guanidine. HCl. The primary assay for activity was the incorporation of 35S-sulfate into proteoglycan in cultures of the fibroblast-like outgrowth cells from explants of neonatal rat muscle. Two Sephacryl S-200 fractions showed enhanced sulfate incorporating activity, but only one showed enhanced incorporation without a concomitant increase in cell number. In the presence of this fraction, the cell cultures produced a larger amount of a new small proteoglycan, as compared to controls, and a significant amount of a much larger proteoglycan. The active fraction had proteins in the Mr range from 8,000 to 15,000 as the major components. These data suggest that the fraction identified may contain the factors responsible for initiating the osteogenic response to dentin matrix upon its implantation in muscle in vivo.
On-demand generation of monodisperse femtolitre droplets by shape-induced shear (2010)

We describe a method for creating discrete femtolitre-scale water-in-oil droplets on demand, based solely on a geometrically induced reduction in oil/water interfacial area at microfabricated junction orifices. This on-demand generation method is driven by self-shear of droplets due to interfacial tension induced forces resulting from a localized transition in microchannel height. The magnitudes of shear stresses involved appear to be significantly less than the shearing instabilities used to split off daughter droplets from aqueous mother plugs at microfabricated junctions in continuous water-in-oil segmented flows, which implies that this method may be better suited for studying biochemical reactions and reaction kinetics in droplets of decreased volume without loss of chemical reactivity due to redistribution of surfactant density used to passivate the oil/water interface. Predictable droplet generation rates under constant pressure conditions or the gated formation of one, two or more droplets at a time with fixed pressure pulses have been demonstrated in a similar manner to active on-demand droplet generation strategies, but with a simpler system not needing actuation and sensing equipment beyond a pressure regulator.
Association of Loci Underlying Field Resistance to Soybean Sudden Death Syndrome (SDS) and Cyst Nematode (SCN) Race 3 (1997)

Coinheritance of field resistance of soybean [ Glycine max (L.) Merr.] to sudden death syndrome (SDS) [caused by the fungus Fusarium solani (Mart.) Sacc. f. sp. phaseoli (Burk.) Snyd. & Hans.] and soybean cyst nematode (SCN) race 3 (caused by Heterodera glycines Ichinohe) sometimes occurs in crosses among adapted cultivars. Our objective was to characterize the loci underlying this coinheritance. One hundred thirty DNA markers were compared with SDS disease response and SCN score among 100 recombinant inbred lines (RILs) derived from a cross between SDS and SCN resistant ‘Forrest’ and SDS and SCN susceptible ‘Essex’. SDS disease incidence (DI) was determined in replicated sites during 4 yr encompassing five locations. The SCN score was determined in the greenhouse from naturally infested field soil samples. Two separate genomic regions identified by random amplified polymorphic DNA (RAPD) markers OI03 450 and OW15 400 were associated with mean SCN score ( P = 0.0001) and jointly accounted for about 47% of variability in SCN score. OI03 450 identified a QTL for resistance to SCN ( R 2 = 14%) within a genomic region that was strongly associated with SDS DI ( R 2 = 20%), partly explaining the coinheritance of the two traits. This locus could be assigned to the region of linkage group G already known to encompass the major SCN resistance locus.
Design and Synthesis of Tubulin and Histone Deacetylase Inhibitor Based on <i>iso</i>-Combretastatin A-4 (2018)

Designing multitarget drugs have raised considerable interest due to their advantages in the treatment of complex diseases such as cancer. Their design constitutes a challenge in antitumor drug discovery. The present study reports a dual inhibition of tubulin polymerization and HDAC activity. On the basis of 1,1-diarylethylenes ( isoCA-4) and belinostat, a series of hybrid molecules was successfully designed and synthesized. In particular compounds, 5f and 5h were proven to be potent inhibitors of both tubulin polymerization and HDAC8 leading to excellent antiproliferative activity.
Mercury Dosing in Fluorescent Lamps (2008)

Low pressure mercury lamps are very efficient light sources, but for the radiation generation they exploit mercury that has a strong negative effect on the environment. Worldwide regulations are pushing for the reduction of mercury content in fluorescent lamps in order to minimize their environmental impact. The trend of Hg content reduction is requiring advanced technological solutions for safe and precise Hg dosing in lamp down to low level. The old liquid mercury dispensing technology, involving risks of contamination with new more reliable and more environmental friendly mercury dosing solutions. The innovative technologies exploiting Hg dispensers based on stable mercury compounds are very reliable solutions for a safe and controlled delivery of small amounts of mercury. The paper will present main methods used to introduce mercury in flourescent lamps, especially focusing on the characteristics of the new Hg dispensers based on inter-metallic compounds that allow to accurately dose mercury even at very low level.
Loop-Size Variation To Probe a Bent Structure of a Hairpin Ribozyme (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTLoop-Size Variation To Probe a Bent Structure of a Hairpin RibozymeYasuo Komatsu, Makoto Koizumi, Haruki Nakamura, and Eiko OhtsukaCite this: J. Am. Chem. Soc. 1994, 116, 9, 3692–3696Publication Date (Print):May 1, 1994Publication History Published online1 May 2002Published inissue 1 May 1994https://pubs.acs.org/doi/10.1021/ja00088a004https://doi.org/10.1021/ja00088a004research-articleACS PublicationsRequest reuse permissionsArticle Views108Altmetric-Citations49LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Generating registration-free cancelable fingerprint templates based on Minutia Cylinder-Code representation (2013)

Cancelable biometrics has become an indispensable technique during biometric authentication. There have been several prior attempts to construct cancelable fingerprint templates. However, existing schemes have not provided a satisfying trade off between performance and security. In this paper, we proposed two novel approaches to construct registration-free cancelable fingerprint templates based on Minutia Cylinder Code, which is the state of the art local representation for fingerprint. And experiments show that the two approaches provide good results in terms of three criteria: performance, unlinkability and irreversibility.
Development of reciprocating magnetic refrigerator. (1985)

The requirement for a helium refrigerator with high efficiency and compactness has been growing for cooling superconducting devices.We have developed a reciprocating magnetic refrigerator for liquefying helium from the temperature of 20K region which uses gadolinium-gallium-garnet (GGG) for the magnetic material. The working material (GGG single crystal, 30mm in diameter and 40mm in length) is placed at the end of the each piston, and those two pistons are driven by a pneumatic jack. When the working material is placed in a high-intensity magnetic field (4.5T), the temperature of the GGG rises to 15K. The inner surface of the cylinder is cooled by the auxiliary refrigerator (a G-M refrigerator), and heat is removed through the narrow gap (less than 50μm) filled with gaseous helium between the GGG and the cylinder. When the magnetic field given to the GGG is eliminated by moving the piston, temperature of the GGG goes below 4.2K. Here the refrigeration of liquid helium occurs by condensing the gaseous helium on the surface of the GGG.Technical emphasis was placed on the realization of a high heat exchange rate between the solid magnetic material and gaseous helium. A sufficient heat transfer rate was achieved after several component-level experiments. Sources of inefficiency to the refrigeration capacity has been also discussed.Finally the refrigeration power of 0.95W at 4.2K was achieved at 0.38Hz operation.
Edaxadiene: A New Bioactive Diterpene from <i>Mycobacterium tuberculosis</i> (2009)

Mycobacterium tuberculosis remains a widespread and devastating human pathogen. Presented here is the characterization of an atypical class I diterpene cyclase from M. tuberculosis that catalyzes an unusual cyclization reaction in converting the known M. tuberculosis metabolite halimadienyl diphosphate to a further cyclized novel diterpene, which we have termed edaxadiene, as it directly inhibits maturation of the phagosomal compartment in which the bacterium is taken up during infection.