gebhart/patrepeval huggingface dataset:
T Lymphocyte‐Mediated Control of Autoimmunity (2007)

Autoreactive T cells can be readily identified in the peripheral lymphocyte pool of both humans and experimental animals. Peripheral tolerance may be maintained by regulatory/suppressor T cells which prevent the activation of autoantigen-specific cells. Mice thymectomized on day 3 of life (d3Tx) develop a wide spectrum of organ-specific autoimmune diseases. Reconstitution of d3Tx mice with CD4+ CD25+ T cells from normal mice prevents the development of disease. Similarly, CD4+ CD25+ T cells prevent the transfer of disease by autoantigen-specific cloned T cells derived from d3Tx mice. Thus, regulatory T cells can prevent both the induction and effector function of autoreactive T cells. In vitro, the CD4+ CD25+ population is anergic to stimulation through the T cell receptor (TCR) and suppresses the proliferative responses of normal CD4+ CD25- cells by a contract-dependent mechanism. Suppression is not MHC-dependent, but requires activation of the CD4+ CD25+ population. The mechanism of suppression in vivo and the target antigen(s) of this unique regulatory population remain to be characterized.
High Concordance of Results of Testing for Human Papillomavirus in Cervicovaginal Samples Collected by Two Methods, with Comparison of a Novel Self-Sampling Device to a Conventional Endocervical Brush (2006)

A user-friendly self-sampling method for collecting representative cervical cell material would lower the threshold for women to respond to the invitation for cervical screening. In the present article, we introduce such a device; we have evaluated its sensitivity and specificity to detect high-grade cervical intraepithelial neoplasia (CIN), via high-risk human papillomavirus (hrHPV) detection and liquid-based cytology (LBC), compared to endocervical brush samples obtained by gynecologists. Women who had a cervical smear reading of moderate dyskaryosis or worse or a repeat equivocal Pap smear result in the cervical screening program (n=64) and healthy volunteers (n=32) took a self-obtained sample at home prior to their visit to the gynecological outpatient department. At the outpatient department, an endocervical brush smear was taken, followed by colposcopy and biopsy whenever applicable. Both self-obtained samples and endocervical brush samples were immediately collected in Surepath preservation solution and used for LBC and hrHPV testing (by general primer-mediated GP5+/6+PCR). hrHPV test results showed a good concordance between the two sample types (87%; kappa=0.71), with sensitivities for prevalent high-grade CIN that did not differ significantly (92% and 95%; P=1.0). The hrHPV test on self-obtained samples proved to be at least as sensitive for high-grade CIN as cytology on endocervical brush samples (34/37 versus 31/37; P=0.5). LBC showed a poor concordance between self-obtained and endocervical brush samples (60%; kappa=0.27). In conclusion, self-obtained samples taken by this novel device are highly representative of the hrHPV status of the cervix. In combination with hrHPV testing, the use of this device may have implications for increasing the attendance rate for cervical screening programs.
Increased expression of H/T-cadherin in tumor-penetrating blood vessels. (2000)

Neovascularization is a prerequisite for tumor growth. Thus, selective destruction of the tumor vasculature should prevent tumor expansion. We have established a method to identify proteins that are specifically expressed on the surface of endothelial cells in tumors. CD31-positive endothelial cells were isolated from Lewis lung carcinoma lung metastases as well as from normal lung tissue. cDNAs derived from these cells were subjected to a subtractive hybridization procedure, and cDNAs overrepresented in tumor-derived endothelial cells were isolated; those encoding surface proteins were selected using a signal sequence trap assay. One isolated cDNA encoded H/T-cadherin. In this report, we show that mouse H/T-cadherin is overexpressed on endothelial cells of several tumors, whereas it is expressed only on a subset of endothelial cells in healthy organs. On the basis of the expression of H/T-cadherin in lung metastases of different tumors, we suggest that different tumors can have a differential influence on the expression of endothelial cell surface proteins.
Human collagen genes encoding basement membrane alpha 1 (IV) and alpha 2 (IV) chains map to the distal long arm of chromosome 13. (1987)

At least 20 genes encode the structurally related collagen chains that comprise greater than 10 homo- or heterotrimeric types. Six members of this multigene family have been assigned to five chromosomes in the human genome. The two type I genes, alpha 1 and alpha 2, are located on chromosomes 17 and 7, respectively, and the alpha 1 (II) gene is located on chromosome 12. Our recent mapping of the alpha 1 (III) and alpha 2 (V) genes to the q24.3----q31 region of chromosome 2 provided the only evidence that the collagen genes are not entirely dispersed. To further determine their organization, we and others localized the alpha 1 (IV) gene to chromosome 13 and in our experiments sublocalized the gene to band q34 by in situ hybridization. Here we show the presence of the alpha 2 type IV locus also on the distal long arm of chromosome 13 by hybridizing a human alpha 2 (IV) cDNA clone to rodent-human hybrids and to metaphase chromosomes. To our knowledge, these studies represent the only demonstration of linkage between genes encoding both polypeptide chains of the same collagen type.
<title>In-situ sensory technique for in-service quality monitoring: measurement of the complex Young's modulus of polymers</title> (1993)

Applications of polymeric adhesives in joining different materials have necessitated quantitative health inspection of adhesive joints (coverage, state of cure, adhesive strength, location of voids, etc.). A new in-situ sensory method has been proposed in this paper to inspect the amount and distribution of the critical constituents of polymers and to measure the characteristic parameters (complex Young's modulus and damping). In this technique, ferromagnetic particles have been embedded in a polymeric matrix, similar to a particle- reinforced composite. The dynamic signatures extracted from the tests as a result of magnetic excitation of the embedded ferromagnetic particles are used to evaluate the complex Young's modulus of the host polymers. Moreover, the amplitude of the frequency response is utilized to identify the amount and distribution of embedded particles in polymeric materials or adhesive joints. The results predicted from the theoretical model agree well with the experimental results. The theoretical analyses and the experimental work conducted have demonstrated the utility of the sensory technique presented for in-service health interrogation.
Investigation of Si IGBT operation at 200 &#x00B0;C for traction application (2011)

In order to satisfy the high density requirement and harsh thermal conditions in future hybrid vehicles, a systematic study of Si IGBT operating at 200°C is performed to determine its feasibility, issues and application guideline. First, the device forward conduction characteristics, leakage current, and switching performance at various temperatures are evaluated through both analytical and lab evaluation. Based on the device characterization, the loss and thermal study is then performed, which provides the guideline for packaging and cooling design. Finally, the possible failure mechanisms at high temperatures including latching and short circuit fault have been tested to ensure the safe and reliable operation of Si IGBTs.
Polarization independent broadband terahertz antireflection by deep‐subwavelength thin metallic mesh (2014)

Abstract Broadband antireflection coatings for passive terahertz (THz) components are extremely important in the application of THz technology. Metallic nano‐films are commonly used for this purpose. Here a new approach to realize polarization independent broadband antireflection in THz range, based on a meta‐surface design is experimentally demonstrated. The internal reflection of a broadband THz pulse (spectral bandwidth of 0.06 – 4 THz) at a Si/air interface can be fully suppressed with a Cr square mesh with deep‐subwavelength dimensions. Small nonuniformity of the meta‐surface structure can enhance the tolerance on structural parameters for achieving the AR condition. The design concept is applicable to other metals and frequency ranges as well, which opens a new window for future AR coatings.
Letter to the editor: diagnostic challenges to be considered regarding Zika virus in the context of the presence of the vector Aedes albopictus in Europe (2016)

Preview this article: Letter to the editor: diagnostic challenges to be considered regarding Zika virus in the context of the presence of the vector Aedes albopictus in Europe, Page 1 of 1 < Previous page | Next page > /docserver/preview/fulltext/eurosurveillance/21/10/eurosurv-21-30161-5-1.gif
Defective endocytic trafficking of NPC1 and NPC2 underlying infantile Niemann-Pick type C disease (2003)

Niemann-Pick type C (NPC) disease is a fatal recessively inherited lysosomal cholesterol-sphingolipidosis. Mutations in the NPC1 gene cause approximately 95% of the cases, the rest being caused by NPC2 mutations. Here the molecular basis of a severe infantile form of the disease was dissected. The level of NPC1 protein in the patient fibroblasts was similar to that in control cells. However, the protein was partially mislocalized from late endocytic organelles diffusely to the cell periphery. In contrast, NPC2 was upregulated and accumulated in cholesterol storing late endocytic organelles. Two point mutations and a four-nucleotide deletion were identified in the NPC1 gene, leading to the amino acid substitutions C113R, P237S and deletion of 37 C-terminal amino acids (delC). Overexpression of individual NPC1 mutations revealed that delC produced an unstable protein, wild-type and NPC1-P237S colocalized with Rab7-positive late endosomes whereas NPC1-C113R localized to the ER, Rab7-negative endosomes and the cell surface. Expression of wild-type or NPC1-P237S cleared the lysosomal cholesterol accumulation in NPC1-deficient cells whereas C113R or delC did not. In the Finnish and Swedish population samples, alleles carrying C113R or delC were not identified, whereas approximately 5% of the alleles carried P237S. Our studies identify P237S as a prevalent NPC1 polymorphism and delC and C113R as deleterious NPC1 mutations. Moreover, they show that delC leads to rapid degradation of NPC1 and C113R to endocytic missorting of the protein. These changes are accompanied by lysosomal accumulation of NPC2, suggesting that NPC1 governs the endocytic transport of NPC2.
Inhibition of Histone Deacetylase 6 (HDAC6) Disrupts the Tolerogenic STAT3 Signaling Pathway and Augments Antitumor Immune Responses in Mantle Cell Lymphoma (MCL) (2012)

Abstract Abstract 3724 MCL is an aggressive and incurable subtype of B-cell Non-Hodgkin's lymphomas. Although patients often respond to first-line chemotherapy plus monoclonal antibodies, relapse and decrease response to further lines of treatment eventually occurs. Manipulation of the immune system to unleash its specificity and long-lasting protective effect might provide a unique opportunity to induce more durable responses or prevent relapse in MCL. Previous studies by our group have shown that HDAC6 is required for production of the immunosuppressive cytokine, IL-10 in antigen-presenting cells (APCs) and that genetic or pharmacologic disruption of HDAC6 in these cells triggers potent antigen-specific T-cell responses. Given the role of IL-10 in suppressing the immunogenicity of B-cells, we asked therefore whether inhibition of HDAC6 with the isotype-selective inhibitor, Tubastatin-A (Tub-A) could reverse the tolerogenic properties of malignant B-cells in a murine model of MCL1. First, in vitro treatment of FC-muMCL1 cells with Tub-A resulted in increased acetylation of a-tubulin, a known HDAC6 target. Treated B-cells also displayed an enhanced expression of MHC class II and the co-stimulatory molecules B7.1, B7.2 and CD40 relative to untreated cells. Such changes resulted in more immunogenic MCL cells able to effectively activate naïve antigen-specific CD4+ T-cells and more importantly, capable of restoring the responsiveness of anergic T-cells from lymphoma-bearing mice. Second, in vivo treatment of FC-muMCL1-bearing C57BL/6 mice with Tub-A resulted in lymphoma rejection. This antitumor effect was not observed in immunodeficient (SCID) C57BL/6 mice treated with Tub-A, pointing to the immunological effects triggered by HDAC6 inhibition in B-cells as playing a dominant role in Tub-A induced anti-MCL activity. Third, mechanistically we have found that HDAC6 physically interacts with STAT3 and it is required for STAT3 phosphorylation and recruitment to the IL-10 gene promoter. Furthermore, Tub-A treated MCL cells displayed a diminished STAT3 phosphorylation and abrogation of IL-10 gene transcriptional activity. Taken together, a concerted regulatory mechanism involving HDAC6 and STAT3 influence the immunogenicity of MCL cells. Targeting HDAC6 with specific inhibitors to disrupt the tolerogenic STAT3/IL-10 axis represents a novel strategy to trigger effective anti-MCL immunity. Disclosures: Martin: Millennium Pharmaceuticals, Inc.: Speakers Bureau.
Roles of uracil-DNA glycosylase and dUTPase in virus replication (2002)

Herpesviruses and poxviruses are known to encode the DNA repair enzyme uracil-DNA glycosylase (UNG), an enzyme involved in the base excision repair pathway that specifically removes the RNA base uracil from DNA, while at least one retrovirus (human immunodeficiency virus type 1) packages cellular UNG into virus particles. In these instances, UNG is implicated as being important in virus replication. However, a clear understanding of the role(s) of UNG in virus replication remains elusive. Herpesviruses, poxviruses and some retroviruses encode dUTPase, an enzyme that can minimize the misincorporation of uracil into DNA. The encoding of dUTPase by these viruses also implies their importance in virus replication. An understanding at the molecular level of how these viruses replicate in non-dividing cells should provide clues to the biological relevance of UNG and dUTPase function in virus replication.
Preconditioned Quantum Linear System Algorithm (2013)

We describe a quantum algorithm that generalizes the quantum linear system algorithm [Harrow et al., Phys. Rev. Lett. 103, 150502 (2009)] to arbitrary problem specifications. We develop a state preparation routine that can initialize generic states, show how simple ancilla measurements can be used to calculate many quantities of interest, and integrate a quantum-compatible preconditioner that greatly expands the number of problems that can achieve exponential speedup over classical linear systems solvers. To demonstrate the algorithm's applicability, we show how it can be used to compute the electromagnetic scattering cross section of an arbitrary target exponentially faster than the best classical algorithm.
Techniques for Pulsed S-Parameter Measurements (2003)

Pulsed measurement is an ticularly power essential tool for measuring devices, are not the performance of power designed to operate con-amplifiers under low duty tinuously or with CW sig-cycle conditions, including nals. This is especially on-wafer test applications true when devices are and high peak-to-average being tested on-wafer, modulation formats where the thermal resistance is greatly increased [1]. In these cases, S-parameter measurements are best performed in a pulsed test environment. The details of pulsed measurement are greatly dependent on the pulse properties being studied. At one extreme is the realm of high pulse repetition frequencies (PRFs) and fairly narrow pulses, as is common in radar applications. At the other extreme is the communications arena where PRFs are quite low and pulse widths fairly wide (e.g., GSM [2]). These two extremes exemplify two techniques, termed bandwidth limited and triggered, that are discussed in this note. To a certain degree, the two approaches overlap in terms of allowed parameters, so most situations can be covered by one if not both of them. The objective of this article is to provide an understanding of general S-parameter measurements performed with a vector network analyzer (VNA), over a range of pulsed conditions for both RF and microwave/mm-wave measurement applications. Pulse profiling of the detailed transient response is not covered here although it is briefly discussed elsewhere with regard to triggered measurements [3]. Certain rise/fall time behaviors can be studied using time domain mode [4] but that will not be discussed either.
SnRK1 activates autophagy via the TOR signaling pathway in Arabidopsis thaliana (2017)

Autophagy is a degradation process in which cells break down and recycle their cytoplasmic contents when subjected to environmental stress or during cellular remodeling. The Arabidopsis thaliana SnRK1 complex is a protein kinase that senses changes in energy levels and triggers downstream responses to enable survival. Its mammalian ortholog, AMPK, and yeast ortholog, Snf-1, activate autophagy in response to low energy conditions. We therefore hypothesized that SnRK1 may play a role in the regulation of autophagy in response to nutrient or energy deficiency in Arabidopsis. To test this hypothesis, we determined the effect of overexpression or knockout of the SnRK1 catalytic subunit KIN10 on autophagy activation by abiotic stresses, including nutrient deficiency, salt, osmotic, oxidative, and ER stress. While wild-type plants had low basal autophagy activity in control conditions, KIN10 overexpression lines had increased autophagy under these conditions, indicating activation of autophagy by SnRK1. A kin10 mutant had a basal level of autophagy under control conditions similar to wild-type plants, but activation of autophagy by most abiotic stresses was blocked, indicating that SnRK1 is required for autophagy induction by a wide variety of stress conditions. In mammals, TOR is a negative regulator of autophagy, and AMPK acts to activate autophagy both upstream of TOR, by inhibiting its activity, and in a parallel pathway. Inhibition of Arabidopsis TOR leads to activation of autophagy; inhibition of SnRK1 did not block this activation. Furthermore, an increase in SnRK1 activity was unable to induce autophagy when TOR was also activated. These results demonstrate that SnRK1 acts upstream of TOR in the activation of autophagy in Arabidopsis.
Exploration Of The Relationship Between LMX And Demographic Variables (2011)

<p class="TX1" style="text-align: justify; line-height: normal; text-indent: 0in; margin: 0in 0.5in 0pt;"><span style="font-family: Times New Roman;"><span style="font-size: 10pt; mso-bidi-font-size: 12.0pt;">This study of 143 participants from an international company examined the relationship between LMX and demographic variables. LMX results represent the quality of the dyadic relationship between leader and follower. Demographic variables included age, gender, education and organizational tenure. Study results suggest significant findings between education and LMX results at the follower level</span><span style="font-size: small;">.</span></span></p>
Pauliceia nas revoluções: artes visuais e agitação política nas primeiras décadas do século XX. (2021)

O artigo trata da luta e mobilização de intelectuais e de artistas, principalmente em São Paulo, a fim de construir uma nova realidade para o País. O que estava em jogo era uma aproximação ousada e perigosa entre arte e política, fazendo com que vários artistas da Semana de Arte Moderna começassem a militar e assumir posicionamentos revolucionários no campo da política. Com a criação dos órgãos de repressão do estado, muitos artistas foram perseguidos ou presos. Muitos intelectuais e artistas de vanguarda tiveram complicações futuras no período Vargas, mas fizeram escolhas nos embates de classes sociais que aconteceram no Brasil. AbstractThe article deals with the struggle and mobilization of intellectuals and artists, especially in São Paulo, in order to build a new reality for the country. What was at stake was a bold and dangerous approach between art and politics, causing several artists of the Week of Modern Art began to military and take revolutionary positions in the field of politics. With the creation of repression agencies, many artists were persecuted or imprisoned. Many avant-garde intellectuals and artists had future complications in the Vargas period, but they made choices in the social class clashes that took place in Brazil.
Dynamic instrumentation of production systems (2004)

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欧阳惠卿教授治疗子宫腺肌病经验介绍 (2014)

Professor Ouyang huiqing is an experienced and excellent doctor of Chinese Medicine in Traditional Guangdong Province, and one of the national tutors of therapeutic experience inheritance. She is an expert in menstruation disorder, pelvic mass, infertility and other difficulty gynecological diseases. She has special view and superior clinical effect in treating adenomyosis. So we introduce her therapeutic experience here.
Westpac's Sustainability Journey: Responding to Community Expectations (2004)

With community attitudes to banks at an all-time low in the late 1990s, Westpac was forced to take action. While the bank's financial realities and responsibilities were not negotiable, it was clear that ignoring community concerns threatened the longer-term sustainability of Westpac's operations. We recognise that as a bank we need not only a legal licence to operate, but also a community or social licence. It is only by delivering to all stakeholders that we will be able to achieve even better results for our shareholders.
Dyschromatopsia in heroin addicts (1990)

The Farnsworth-Munsell 100-hue test was employed to determine whether there was any defect of colour vision in 29 confirmed male heroin addicts who had been successfully detoxified. Forty age-matched males served as controls. A typical normal error score on the FM test is about 40 and an error score of over 100 indicates poor colour discrimination; 86.2% of the eyes of the control group had an error score below 100 while only 17.2% of the eyes of heroin addicts had an error score below 100. The colour confusion among the heroin addicts was in the blue-purple (475-495 mu) range. These results indicate that colour vision defects are more common in heroin addicts.
Energetics of nanoparticle oxides: interplay between surface energy and polymorphism† (2003)

Many oxides tend to form different structures (polymorphs) for small particles. High temperature oxide melt solution calorimetry has been used to measure the enthalpy as a function of polymorphism and surface area for oxides of Al, Ti, and Zr. The results confirm crossovers in polymorph stability at the nanoscale. The energies of internal and external surfaces of zeolitic silicas with open framework structures are an order of magnitude smaller than those of oxides of normal density.
Biomass Carbon Removal and Storage (BiRCS) Roadmap (2021)

This roadmap introduces a new term: biomass carbon removal and storage (BiCRS). The term describes a range of processes that use plants and algae to remove carbon dioxide (CO2) from the atmosphere and store that CO2 underground or in long-lived products.