gebhart/patrepeval huggingface dataset:
Anxiety- and Depression-Like States Lead to Pronounced Olfactory Deficits and Impaired Adult Neurogenesis in Mice (2016)

Numerous clinical reports underscore the frequency of olfactory impairments in patients suffering from major depressive disorders (MDDs), yet the underlying physiopathological mechanisms remain poorly understood. We hypothesized that one key link between olfactory deficits and MDD lies in hypercortisolemia, a cardinal symptom of MDD. Corticosterone (CORT) is known to negatively correlate with hippocampal neurogenesis, yet its effects on olfactory neurogenesis and olfaction remain unknown. Here we used a rodent model of anxiety/depression-like states, which is based on chronic CORT administration and studied the effects of the antidepressant fluoxetine (FLX) on behavior, olfaction, and adult neurogenesis in the dentate gyrus (DG), olfactory bulb (OB), and the olfactory epithelium (OE). Chronic CORT had no effect on cell proliferation in the OE or on olfactory sensory neurons projecting to the OB, but induced pronounced deficits in olfactory acuity, fine discrimination of odorants and olfactory memory. These alterations were accompanied by a significant decrease in the number of adult-born neurons in both the DG and OB. Remarkably, FLX not only reversed depression-like states as expected, but also improved olfactory acuity, memory, and restored impaired adult neurogenesis. However, fine olfactory discrimination was not restored. Morphological analysis of adult-born neurons in both the DG and the OB showed that dendritic complexity was not significantly affected by CORT, but was increased by FLX. These findings demonstrate an essential role for glucocorticoids in triggering olfactory impairments in MDD and highlight a novel therapeutic effect of FLX. SIGNIFICANCE STATEMENT Increasing clinical reports show that major depression is characterized by pronounced olfactory deficits, yet the underlying mechanisms remain unknown. In this work, we used an endocrine model of depression to study whether hypothalamic-pituitary-adrenal axis perturbation could be sufficient to provoke olfactory impairments. We found that chronic corticosterone not only induces marked deficits in olfactory acuity, fine discrimination and olfactory memory, but also significantly decreases bulbar and hippocampal neurogenesis. Importantly, the antidepressant fluoxetine restores both adult neurogenesis and depressive states, and improves most olfactory functions. Our data reveal that impairment of hypothalamic-pituitary-adrenal axis during depression can lead to olfactory deficits and that the neurogenic effects of selective serotonin reuptake inhibitor antidepressants can successfully restore certain olfactory functions.
Abdominal Magnetic Resonance Elastography (2009)

Magnetic resonance elastography (MRE) is a magnetic resonance imaging-based technique for quantitatively assessing the mechanical properties of tissues based on the propagation of shear waves. Multiple studies have described many potential applications of MRE, from characterizing tumors to detecting diffuse disease processes. Studies have shown that MRE can be successfully implemented to assess abdominal organs. The first clinical application of MRE to be well documented is the detection and characterization of hepatic fibrosis, which systematically increases the stiffness of liver tissue. In this diagnostic role, it offers a safer, less expensive, and potentially more accurate alternative to invasive liver biopsy. Emerging results suggest that measurements of liver and spleen stiffness may provide an indirect way to assess portal hypertension. Preliminary studies have demonstrated that it is possible to use MRE to evaluate the mechanical properties of other abdominal structures, such as the pancreas and kidneys. Steady technical progress in developing practical protocols for applying MRE in the abdomen and the pelvis provides opportunities to explore many other potential applications of this emerging technology.
An artificial self‐sufficient cytochrome P450 directly nitrates fluorinated tryptophan analogs with a different regio‐selectivity (2016)

Aromatic nitration is an immensely important industrial process to produce chemicals for a variety of applications, but it often suffers from multiple unsolved challenges. Enzymes as biocatalysts have been increasingly used for organic chemistry synthesis due to their high selectivity and environmental friendliness, but nitration has benefited minimally from the development of biocatalysis. In this work, we aimed to develop TxtE as practical biocatalysts for aromatic nitration. TxtE is a unique class I cytochrome P450 enzyme that nitrates the indole of l-tryptophan. To develop cost-efficient nitration processes, we fused TxtE with the reductase domains of CYP102A1 (P450BM3) and of P450RhF to create class III self-sufficient biocatalysts. The best engineered fusion protein was comparable with wild type TxtE in terms of nitration performance and other key biochemical properties. To demonstrate the application potential of the fusion enzyme, we nitrated 4-F-dl-tryptophan and 5-F-l-tryptophan in large scale enzymatic reactions. Tandem MS/MS and NMR analyses of isolated products revealed altered nitration sites. To our knowledge, these studies represent the first practice in developing biological nitration approaches and lay a solid basis to the use of TxtE-based biocatalysts for the production of valuable nitroaromatics.
Anti-cancer activity of hierarchical ZSM-5 zeolites synthesized from rice-based waste materials (2017)

We report the organic template-free synthesis of hierarchical ZSM-5 zeolite using waste materials, by a simple hydrothermal method and their excellent anti-cancer activity against A549 human lung epithelial cell lines.
High-performance circuit testing with slow-speed testers (2002)

We propose a method of testing high-speed digital devices whose clock frequency exceeds the capability of the test equipment. The circuit is designed such that a controllable delay is introduced in the timing paths during test. With the added delay, the maximum operating frequency is lowered to a rate which is within the capability of the ATE. The delay circuit is so designed that its function is also testable. In an illustrative design with single clock, the controllable delay is incorporated within a master-slave flip-flop. The control of delay is then achieved by manipulation of the duty-cycle of the clock waveform. In a two-clock system, no modification of the flip-flop is required and the delay is varied by skewing one clock signal with respect to the other.
Three-section semiconductor-laser amplifier for monitoring of optical gain (1993)

Semiconductor-laser amplifiers (SLA’s) can perform many optical-signal-processing functions. In addition to loss compensation and switching, a SLA can simultaneously amplify and detect an optical signal.1 Recently, two-contact amplifiers have demonstrated improved detection responsivi ty.23 Here, we show that a three-section SLA can further be used to monitor amplifier gain.
A comparison of the open loop transmit diversity schemes for third generation wireless systems (2002)

Multiple transmit antennas giving rise to diversity (transmit diversity) have been shown to increase the downlink (base station to the mobile) capacity in cellular systems. The third generation partnership project (3Gpp) for WCDMA FDD has chosen space time transmit diversity (STTD) as the open loop transmit diversity technique for two transmit antennas. On the other hand, the CDMA2000 has chosen space time spreading (STS) and orthogonal transmit diversity (OTD) as the open loop transmit diversity techniques. In this paper, we compare the open loop transmit diversity techniques of 3Gpp and CDMA2000. We show that STS involves a QPSK to non-QPSK constellation transformation followed by an STTD encoding of the resulting symbols. Thus, the performance of STS is the same as STTD, while the peak to average ratio (PAR) and complexity of STS is higher than STTD. We also show theoretically that the performance of STTD is 0.3-0.5 dB better than OTD. Further, STTD has another distinct advantage over OTD and STS in its application to the broadcast channels. During the power on and possibly during soft handoff, the mobile does not know the presence/absence of the diversity antenna on the primary/soft handoff base station. In this case, a blind detection of the diversity antenna is possible for STTD with a very small degradation in performance. This is not possible for OTD and STS. We thus show that STTD is a better open loop transmit diversity technique than OTD and STS.
BIOLOGY AND GENETICS OF PRION DISEASES (1994)

Enriching fractions from Syrian hamster (SHa) brain for scrapie prion infectivity led to the discovery of the prion protein (PrP). Prion diseases include scrapie of sheep, bovine spongiform encephalopathy (BSE) of cattle, as well as Creutzfeldt-Jakob disease (CJD), Gerstmann-Sträussler-Scheinker disease (GSS), and fatal familial insomnia (FFI) of humans. Discovery of mutations in the PrP genes of humans with familial CJD, GSS, and FFI established that prion diseases are both genetic and infectious. Many lines of evidence have converged to argue that infectious prions are composed largely, if not entirely, of PrPSc molecules. Mice overexpressing mutant and wild-type transgenes develop neurologic illnesses spontaneously and produce prions as demonstrated by serial transmission of disease in rodents after inoculation of brain extracts. Although these and many other findings argue that prions are devoid of nucleic acid, the molecular basis of prion strains remains enigmatic. The formation of PrPSc from PrPC is a posttranslational process involving the conversion of alpha-helices into beta-sheets. This conformational change in PrP appears to be the fundamental event that underlies prion propagation and the pathogenesis of prion diseases. The unique features of prion structure and propagation differentiate prions from all other transmissible pathogens.
Nutrient intake and urine composition in calcium oxalate stone-forming dogs: comparison with healthy dogs and impact of dietary modification. (2004)

Nutrient intake and urine composition were analyzed in calcium oxalate (CaOx)stone-forming and healthy control dogs to identify factors that contribute to CaOx urolithiasis. Stone-forming dogs had significantly lower intake of sodium, calcium, potassium, and phosphorus and significantly higher urinary calcium and oxalate concentrations, calcium excretion, and CaOx relative supersaturation (RSS). Feeding a diet used in the treatment of canine lower urinary tract disease for 1 month was associated with increased intake of moisture, sodium, and fat; reduced intake of potassium and calcium; and decreased urinary calcium and oxalate concentrations, calcium excretion, and CaOx RSS. No clinical signs of disease recurrence were observed in the stone-forming dogs when the diet was fed for an additional 11 months. The results suggest that hypercalciuria and hyperoxaluria contribute to the formation of CaOx uroliths in dogs and show that dietary modifications can alter this process.
Robust Transmission for Massive MIMO Downlink With Imperfect CSI (2019)

In this paper, the design of robust linear precoders for the massive multi-input-multi-output (MIMO) downlink with imperfect channel state information (CSI) is investigated. The imperfect CSI for each UE obtained at the BS is modeled as statistical CSI under a jointly correlated channel model with both channel mean and channel variance information, which includes the effects of channel estimation error, channel aging, and spatial correlation. The design objective is to maximize the expected weighted sum-rate. By combining the minorize-maximize (MM) algorithm with the deterministic equivalent method, an algorithm for robust linear precoder design is derived. The proposed algorithm achieves a stationary point of the expected weighted sum-rate maximization problem. To reduce the computational complexity, two low-complexity algorithms are then derived. One for the general case, and the other for the case when all the channel means are zeros. For the later case, it is proved that the beam domain transmission is optimal, and thus the precoder design reduces to the power allocation optimization in the beam domain. Simulation results show that the proposed robust linear precoder designs apply to various mobile scenarios and achieve high spectral efficiency.
An Interframe Coding Scheme for Packet Video (1989)

Interframe coding schemes though sensitive to the propagative affects of channel-errors, are much more efficient than intraframe coding schemes, when using similar data-compression techniques. Moreover, since in interframe coding, the instantaneous data-rate may vary drastically, it can benefit from the bits-on-demand property of packet-networks. We propose a layered coding-structure that contains a self-contained basic inter-frame codec as the core, and supplementary additive extended-codecs. The inter-frame codec uses a slightly modified version of motion-compensated DCT coding scheme proposed by the CCITT in reference model 8 for pX64kbit/ s videophone. It encodes the lower spatial resolution image sequence, with an adequate temporal resolution. The encoded-data it produces is assigned the highest priority. The extended-codecs allow enhancement of temporal and spatial resolutions, at the cost of increase in the data-rate. They encode the interpolative differences between the upsampled local decoded output of the basic-codec and the higher resolution input. The encoded-data of the extended codecs is assigned lower priority.
High-Sensitivity Image-Sensor-Incorporated Image Intensifier Tube with Charge-Coupled Device for X-Ray Diffraction (1993)

The construction and performance of the two-dimensional X-ray detector are described for X-ray diffraction. The detector consists of an input fluorescent screen, an image intensifier tube and a charge-coupled device (CCD) image sensor, which is coupled using fiber-optics. The background noise of the detector is about 10 -3 of the saturation peak, and the linearity range is about two orders of magnitude for the incident X-ray photons. The observation of the diffraction images with a low X-ray dose is demonstrated using this detector.
Extensions for the Foresighted Driver Model: Tactical lane change, overtaking and continuous lateral control (2016)

The Foresighted Driver Model (FDM) is a microscopic driver model which is based on the idea that a driver balances risk with utility. This paper deals with the modeling of advanced driving maneuvers for the FDM with a special focus on lateral positioning scenarios, such as lane changes in highway traffic. When driving at high speeds, tactical preparation for a safe lane change is of high importance. In this context, the paper presents maneuvers that allow for lane changes to be planned well in advance and carefully made without the restraint of requiring immediate action. Furthermore, the paper presents a continuous lateral control which allows driving on arbitrary paths other than the centerline, depending on the current traffic situation. Since more complex lateral maneuvers require more detailed considerations of the environment, an approach is presented to model the lane and the environmental influences. This paves the way for a modeling of variables such as lane markings, roadblocks, hard shoulders and more. Simulations illustrate how the introduced maneuvers allow successful preparation for upcoming lane changes and how traffic obstructions can be bypassed without performing a lane change but by using the continuous lateral control.
Hyperthermal neutral beam etching (1995)

A pulsed beam of hyperthermal fluorine atoms with an average translational energy of 4.8 eV has been used to demonstrate anisotropic etching of Si. For 1.4 Hz operation, a room-temperature etch rate of 300 Å/min for Si(100) has been measured at a distance of 30 cm from the source. A 14% undercutting for room-temperature etching of Novolac-masked Si features was achieved under single-collision conditions, with no detectable mask erosion. Translational energy and angular distributions of scattered fluorine atoms during steady-state etching of Si by a normal-incidence, collimated beam demonstrate that unreacted F atoms can scatter inelastically, retaining a significant fraction of their initial kinetic energies. The observed undercutting can be explained by secondary impingement of these high-energy F atoms, which are more reactive upon interaction with the sidewalls than would be expected if they desorbed from the surface at thermal energies after full accommodation. Time-of-flight distributions of volatile reaction products were also collected, and they show evidence for a dominant nonthermal reaction mechanism of the incident atoms with the surface in addition to a thermal reaction channel.
A Novel Approach to Gas Separations Using Composite Hollow Fiber Membranes (1980)

Abstract A method has been developed by which a porous hollow fiber which cannot separate gases to a significant extent can be made to exhibit its intrinsic separation properties by coating with an appropriate material. A unique feature of this composited fiber is that the separation properties are determined by the porous support polymer rather than by the coating polymer. The hollow fibers produced by this method have extraordinarily high rates compared to earlier hollow fibers used for gas separations. In addition, they can function under extremely high pressure gradients. Gases such as He, H2, and CO2 can be separated from gases like CH4, CO, and N2, and the system is chemically and physically stable to a wide range of typical industrial contaminants. As a result, systems based on these fibers should be useful in a variety of processes, some of which include stream splitting, gas composition control, H2 upgrading, and purge gas recovery.
Photorefractive acousto-optical imaging for thick biological tissues (2005)

A new concept of acousto-optical imaging is emerging based on an interferometric setup containing a photorefractive crystal as the recombination plate, and a single detector. This wavefront-adaptive holography technique is promising since the measurements are made in real-time with a high flux collection and at a high rate, faster than the speckle decorrelation time. We present here a detailed model that describes correctly the measured signal, whether in a temporal phase or amplitude modulation of the ultrasound.
The use of cytokines, fusion proteins and antibodies to treat cutaneous T-cell lymphoma (2003)

It has long been known that certain immune augmenting therapeutics, particularly interferon alpha, can exert profound salutary effects on the clinical progress of patients with cutaneous T-cell lymphoma. Emerging evidence that the host immune response may play an important role in the control of this disorder has led to the clinical application of other cytokines including interleukin-12 and interferon gamma. In this review, the authors will summarize current knowledge regarding the use of cytokines, fusion proteins and antibodies for the treatment of cutaneous T-cell lymphoma.
Alloy Design of Nb-Based Hydrogen Permeable Membrane with Strong Resistance to Hydrogen Embrittlement (2008)

The alloying effects of Ru and W on the hydrogen solubility, the resistance to hydrogen embrittlement and hydrogen permeability are investigated quantitatively for Nb-based hydrogen permeable alloys. It is found that the hydrogen solubility decreases by the addition of alloying element into niobium or by increasing the temperature. As a result, the resistance to hydrogen embrittlement is improved by reducing the hydrogen concentration. On the other hand, the hydrogen flux, J, through the alloy membrane increases linearly with increasing difference of hydrogen concentration, ΔC, between both sides of the membrane. It is shown that the Nb-5 mol%X (X = Ru and W) alloys possess excellent hydrogen permeability without showing any hydrogen embrittlement when used under appropriate permeation conditions, i.e. temperature and hydrogen pressures. Also, the hydrogen diffusion coefficients during the practical hydrogen permeation at high temperature are evaluated from the linear relationship between the hydrogen flux and the hydrogen concentration difference. It is found that the hydrogen diffusion coefficient of pure Nb is much lower than the reported values measured for dilute hydrogen solid solutions. Surprisingly, the hydrogen diffusion is found to be faster in Pd-26 mol%Ag alloy with fcc crystal structure than in pure niobium with bcc structure at 773 K during the hydrogen permeation. It is also interesting that the addition of Ru or W into niobium enhances the hydrogen diffusion of the practical hydrogen permeation at high temperature.
Rapid pruritus reduction with ruxolitinib cream treatment in patients with atopic dermatitis (2022)

Ruxolitinib cream demonstrated rapid improvement in itch in patients with mild to moderate AD that was sustained for 8 weeks. Significantly more patients applying ruxolitinib cream achieved itch NRS2 within approximately 12 h and itch NRS4 by Day 2 versus vehicle.
ZFAT is an antiapoptotic molecule and critical for cell survival in MOLT‐4 cells (2009)

ZFAT (also known as ZNF406), originally identified as a candidate gene for autoimmune thyroid disease, encodes a zinc-finger protein, however, its function has not been elucidated. Here, we report that human ZFAT protein is expressed in peripheral B and T lymphocytes and a human acute T lymphoblastic leukaemia cell line, MOLT-4 cells. Intriguing is that mouse ZFAT expression in CD4(+) lymphocytes is increased during blast formation. Furthermore, ZFAT-knockdown in MOLT-4 induces apoptosis via activation of caspases. These results suggested that ZFAT protein is a critical regulator involved in apoptosis and cell survival for immune-related cells.
A Unified Methodological Framework for Vestibular Schwannoma Research (2017)

Vestibular schwannomas are the most common neoplasms of the cerebellopontine angle, making up 6-8% percent of all intracranial growths. Though these tumors cause sensorineural hearing loss in up to 95% of affected individuals, the molecular mechanisms underlying this hearing loss remain elusive. This article outlines the steps established in our laboratory to facilitate the collection and processing of various primary human tissue samples for downstream research applications integral to the study of vestibular schwannomas. Specifically, this work describes a unified methodological framework for the collection, processing, and culture of Schwann and schwannoma cells from surgical samples. This is integrated with parallel processing steps now considered essential for current research: the collection of tumor and nerve secretions, the preservation of RNA and the extraction of protein from collected tissues, the fixation of tissue for the preparation of sections, and the exposure of primary human cells to adeno-associated viruses for application to gene therapy. Additionally, this work highlights the translabyrinthine surgical approach to collect this tumor as a unique opportunity to obtain human sensory epithelium from the inner ear and perilymph. Tips to improve experimental quality are provided and common pitfalls highlighted.