gebhart/patrepeval huggingface dataset:
Witnessing aggression: the aftershock: Complex symptoms shroud violence-induced depression (1996)

Long after a bullet wound or a bruised face has healed, children and adolescents can remain traumatized by what they9ve seen. Whether it9s a mother9s repeated beatings at the hands of a domestic partner, a neighborhood drive-by shooting, or TV and movie violence, assessing the impact of witnessing such acts can prove difficult. The single most important public health problem today, as I see it, is violence in our society, said Hyman Tolmas, M.D., a member of the AAP Committee on Psychosocial Aspects of Child and Family Health (COPACFH). It9s totally destructive, and the damage runs deep.
Molecular Model of Human β-Cell Glucokinase Built by Analogy to the Crystal Structure of Yeast Hexokinase B (1994)

Recent studies have shown that mutations in human beta-cell glucokinase that impair the activity of this key regulatory enzyme of glycolysis can cause early-onset non-insulin-dependent diabetes mellitus (NIDDM). The amino acid sequence of human glucokinase has 31% identity with yeast hexokinase, a related enzyme for which the crystal structure has been determined. This homology has allowed us to model the three-dimensional structure of human glucokinase by analogy to the crystal structure of yeast hexokinase B. This model of human glucokinase provides a basis for understanding the effects of mutations on its enzymatic activity. Residues in the active site and on the surface of the binding cleft for glucose are highly conserved in both enzymes. Regions far from the active site are predicted to differ in conformation, and 10 insertions or deletions that range in size from 1 to 7 residues are located on the protein surface between elements of secondary structure. The model structure suggests that human glucokinase binds glucose in a similar manner to yeast hexokinase. The glucose-binding site contains a conserved aspartic acid, two conserved glutamic acids, and two conserved asparagines that form hydrogen bond interactions with the hydroxyls of the glucose similar to those observed in other sugar-binding proteins. Mutation of residues in the predicted glucose-binding site has been found to greatly reduce enzymatic activity. This model will be useful for future structure/function studies of glucokinase.
ACRL Board of Directors’ actions, June 2003: Highlights of the Board’s Annual Conference meetings (2003)

in Toronto, the ACRL Board of Direc tors met on June 21 and 24 and took the actions.The actions appear here along with the strategic directions that they ad fodres ll o w i n g s.Strategic Direction 1.0: Society recognizes the contributions that academic and research libraries and librarians make in higher education, scholarly communication, and civic development.Approved Rinding of $5,000 for library workshops Awards and recognitionA p p ro ved a re so lu tio n th a n k in g K ate C o rb y and EBSS for their leadership and dedicated ef forts to preserve the value of ERIC as a resource for the educational community. A p p ro v e d a re s o lu tio n c o n g ra tu la tin g an dthanking LES and DLS for their work in develop ing a mission and strategic plan that meets die needs of their respective members and that moves for ward the work of the sections and the association.for Council of Independent College institutions.
Design of Halbach-Array-Based Permanent-Magnet Motors With High Acceleration (2011)

The Halbach magnetized permanent-magnet (PM) motors can be designed to achieve high torque density and to realize hollow rotor structure with very low rotational inertia. In this paper, the design and analysis of Halbach-array-based PM motors, which can produce high output torque and meet the requirements for very fast dynamic performance, are presented. A design method is proposed to select the optimum dimensions of the Halbach-array-based PM machines. The analysis and design approach for the selection of the suitable stator winding distribution, while considering the effect of the end windings, are also presented. Loss analysis of the machine is carried out, and a simplified 3-D model is proposed for its thermal analysis. Detailed finite-element analysis results are provided for the verification of the performances of the designed machine.
Designing Randomized DNA Sequences Free of Restriction Enzyme Recognition Sites (2017)

DNA libraries containing random "barcodes" complicate synthetic biology workflows that utilize restriction enzymes since restriction sites can appear inside some barcodes. By removing bases at particular sites in the barcodes, it is possible to create semi-random pools of barcodes that do not contain any restriction sites. The challenge is to remove as few bases as possible to maximize the number of sequences in the pool while ensuring all sequences are free of restriction sites. The authors present CutFree, a computational approach to create pools of random DNA barcodes that lack a pre-defined set of restriction sites. The resulting pools can be inexpensively produced en masse with standard DNA synthesis techniques. CutFree is experimentally validated by blocking digestion of pools of barcodes designed to frequently contain restriction sites. Using CutFree, a pool of 1.3 billion barcodes that are free from recognition sites for 182 commercially available restriction enzymes is designed. CutFree is available as a software package and an online tool (http://jensenlab.net/tools).
Building Nanocomposite Magnets by Coating a Hard Magnetic Core with a Soft Magnetic Shell (2014)

Controlling exchange coupling between hard magnetic and soft magnetic phases is the key to the fabrication of advanced magnets with tunable magnetism and high energy density. Using FePt as an example, control over the magnetism in exchange-coupled nanocomposites of hard magnetic face-centered tetragonal (fct) FePt and soft magnetic Co (or Ni, Fe2C) is shown. The dispersible hard magnetic fct-FePt nanoparticles are first prepared with their coercivity (Hc) reaching 33 kOe. Then core/shell fct-FePt/Co (or Ni, Fe2C) nanoparticles are synthesized by reductive thermal decomposition of the proper metal precursors in the presence of fct-FePt nanoparticles. These core/shell nanoparticles are strongly coupled by exchange interactions and their magnetic properties can be rationally tuned by the shell thickness of the soft phase. This work provides an ideal model system for the study of exchange coupling at the nanoscale, which will be essential for building superstrong magnets for various permanent magnet applications in the future.
VERDICT: The Verapamil versus Digoxin Cardioversion Trial: A Randomized Study on the Role of Calcium Lowering for Maintenance of Sinus Rhythm after Cardioversion of Persistent Atrial Fibrillation (2001)

Stand-alone intracellular calcium lowering by verapamil around ECV does not enhance cardioversion outcome.
Nanostructured silica as a support for regenerable high-capacity organoamine-based CO2 sorbents (2010)

Nanostructured precipitated and fumed silica (nanosilica) were found to be superior supports for the immobilization of various organoamines. The easily prepared solids were found to be regenerable and effective CO2 absorbents at moderate temperatures from room temperature up to 100 °C. Supported polyethylenimines (PEIs), especially branched low-molecular-weight (LMW, Mwca. 800) and high-molecular-weight (HMW, Mwca. 25 000) PEIs were particularly suitable as CO2 absorbents. Amines with lower molecular weights and lower boiling points such as pentaethylenehexamine (PEH), tetraethylenepentamine (TEP), monoethanolamine (MEA) and diethanolamine (DEA) showed sorbent leaching from the solid absorbents. The addition of poly(ethylene glycol) was found to significantly enhance the subsequently needed desorption of CO2 from PEI-based solid absorbents. In the preparation of CO2 absorbents, the particle size of the nanosilica was found to be of advantage compared to other solid supports having similar or higher surface area but larger particle size.
Induction and characterization of a novel amine oxidase from the yeast <i>Kluyveromyces marxianus</i> (2003)

An amine oxidase from the yeast Kluyveromyces marxianus was induced, purified and completely characterized; it was shown to belong to the class of copper-containing amine oxidases (E.C. 1.4.3.6). The enzyme was induced by putrescine and, very strongly, by copper(II); structural-functional characterization of the enzyme was performed, including determination of molecular weight, glycosylation, copper and TPQ content, isoelectric point, K(M) and k(CAT) (with benzylamine as substrate), pH, temperature and ionic strength effect on catalysis, substrate and inhibitor specificity. A 700 bp clone was isolated containing the cDNA that encodes for the C-terminus of the enzyme; the amino acid sequence deduced (the first available for a benzylamine oxidase from yeast) was compared to that of other copper amine oxidases from microorganisms and higher organisms. From the results obtained, the putrescine/benzylamine oxidase from Kluyveromyces marxianus was found to have a good homology with other enzymes of this class from microorganisms, and particularly with AO I from Aspergillus niger. Nonetheless, some features resulted closer to those of animal amine oxidases and histaminases. Some potential biotechnological applications are proposed. The cDNA Accession No. is AJ320485.
Synthesis, pro-apoptotic activity and 2D-QSAR studies of new analogues of fluphenazine. (2014)

A series of 10 novel analogues of fluphenazine (FPh) were synthesized. Influence of the synthesized analogues of FPh on frequency of apoptosis and necrosis in cultures of human lymphocytes genotoxically damaged in vitro with benzo[a]pyrene (B[a]P; 7,5 microM, 48 h) was compared with the effect of FPh. Activity of the tested compounds was expressed by ED50 (pro-apoptotic activity) and TD50 (pro-necrotic effect, cytotoxicity). It was noticed that compounds 3-9 and 12 exerted a pro-apoptotic effect markedly stronger than that of FPh. Additionally, compounds 3, 9 and 10 exhibited the weakest influence on frequency of necrotic lymphocyte in cultures. 2D-QSAR analysis was done in order to find quantitative relationship between structures of the tested analogues and their pro-apoptotic activity or pro-necrotic effect in B[a]P-damaged cell cultures. Several statistically significant QSAR models were generated. Information obtained from 2D-QSAR study will be used in further design of analogues of FPh more active in cancer chemoprevention.
Background File Transfer Program (BFTP) (1988)

This RFC describes an Internet background file transfer service that
is built upon the third-party transfer model of FTP. No new protocols
are involved. The purpose of this memo is to stimulate discussions on
new Internet service modes.
A framework for design space exploration of parameterized VLSI systems (2003)

The paper presents two new approaches to multi-objective design space exploration for parametric VLSI systems. Both considerably reduce the number of simulations needed to determine the Pareto-optimal set as compared with an exhaustive approach. The first uses sensitivity analysis while the second uses evolutionary computing techniques. Application to a highly parametric system-on-a-chip for digital camera applications shows the validity of the methodologies presented in terms of both accuracy of results and efficiency, measured as the number of simulations needed to determine the power/execution-time trade-off front.
A Single-Stage PFM-APWM Hybrid Modulated Soft-Switched Converter With Low Bus Voltage for High-Power LED Lighting Applications (2017)

In order to design a high-efficiency cost-effective high-power LED driver, a single-stage integrated boost- LLC-type soft-switched ac-dc converter is usually employed. The minimized number of semiconductors and the reduced power loss are its main advantages. However, the high bus voltage is its well-known disadvantage, which is caused by the employment of a conventional pulse frequency modulation (PFM) control strategy with the duty cycle of 0.5. The range of the input ac voltage is severely limited. In order to overcome this problem, a hybrid PFM-asymmetric pulse width modulation strategy is proposed in this paper. The proposed strategy can significantly reduce the bus voltage while maintaining the soft-switching operation for both primary MOSFETs and secondary diodes. Furthermore, the electrolytic capacitors are removed by increasing the bus voltage ripple. Hence, high efficiency, long lifetime, low cost, and universal operation are achieved on the proposed LED driver, which is well matched to the virtues of LED lighting source. The detailed operation principles and design consideration for the proposed modulation approach are analyzed and discussed. The feature of the proposed solution was demonstrated using a universal-input 100-W hardware prototype. The experimental results verified the full-range zero-voltage-switching (ZVS) operation for primary switches and the efficiency achieved 91.6% under the 130-V input voltage.
Separation of lipid and water MR imaging signals by chopper averaging in the time domain. (1987)

A convenient alternative to Dixon's water/lipid imaging technique is proposed. The sequence exploits the automatic paired averaging capability that is used in commercial magnetic resonance imaging systems for data averaging to achieve lipid suppression. This can be easily implemented via a minor modification to an existing partial saturation sequence. This approach provides linear suppression of lipid signals that is independent of the relative abundance of water and lipid contributions, unlike the characteristics of the original Dixon sequence. The proposed sequence can be tuned before the imaging procedure to ensure optimum timing and frequency adjustment.
Development of an immunocontraceptive vaccine. Current status. (1999)

Development of an effective and modern contraceptive vaccine is a key factor in the global issue of regional population growth as well as agricultural, medical, economic and social development. A review was done of the current medical literature concerning development of an immunocontraceptive vaccine and relative molecular biology technology. Various approaches have been taken to identify candidate-specific antigens for immunocontraceptive development, such as sperm, zona pellucida and hormonal antigens. Suppressed fertility and the reversibility of these effects on mammalian species, including humans, have been demonstrated. The successful results obtained so far support the continued investigation for an effective immunocontraceptive vaccine.
Analysis of Two New Arabinosyltransferases Belonging to the Carbohydrate-Active Enzyme (CAZY) Glycosyl Transferase Family1 Provides Insights into Disease Resistance and Sugar Donor Specificity (2018)

Glycosylation of small molecules is critical for numerous biological processes in plants, including hormone homeostasis, neutralization of xenobiotics, and synthesis and storage of specialized metabolites. Glycosylation of plant natural products is usually performed by uridine diphosphate-dependent glycosyltransferases (UGTs). Triterpene glycosides (saponins) are a large family of plant natural products that determine important agronomic traits such as disease resistance and flavor and have numerous pharmaceutical applications. Most characterized plant natural product UGTs are glucosyltransferases, and little is known about enzymes that add other sugars. Here we report the discovery and characterization of AsAAT1 (UGT99D1), which is required for biosynthesis of the antifungal saponin avenacin A-1 in oat (<i>Avena strigosa</i>). This enzyme adds l-Ara to the triterpene scaffold at the C-3 position, a modification critical for disease resistance. The only previously reported plant natural product arabinosyltransferase is a flavonoid arabinosyltransferase from Arabidopsis (<i>Arabidopsis thaliana</i>). We show that AsAAT1 has high specificity for UDP-β-l-arabinopyranose, identify two amino acids required for sugar donor specificity, and through targeted mutagenesis convert AsAAT1 into a glucosyltransferase. We further identify a second arabinosyltransferase potentially implicated in the biosynthesis of saponins that determine bitterness in soybean (<i>Glycine max</i>). Our investigations suggest independent evolution of UDP-Ara sugar donor specificity in arabinosyltransferases in monocots and eudicots.
Learning Curve in Tracheal Allotransplantation (2012)

The first vascularized tracheal allotransplantation was performed in 2008. Immunosuppression was stopped after forearm implantation and grafting of the recipient mucosa to the internal site of the transplant. Nine months after forearm implantation, the allograft was transplanted to the tracheal defect on the radial blood vessels. Since then, four additional patients have undergone tracheal allotransplantation, three (patients 2-4) for long-segment stenosis and one (patient 5) for a low-grade chondrosarcoma. Our goal was to reduce the time between forearm implantation and orthotopic transplantation and to determine a protocol for safe withdrawal of immunosuppressive therapy. Following forearm implantation, all transplants became fully revascularized over 2 months. Withdrawal of immunosuppression began 4 months after graft implantation and was completed within 6 weeks in cases 2-4. Repopulation of the mucosal lining by recipient cells, to compensate for the necrosis of the donor mucosa, was not complete. This resulted in partial loss of the allotransplant in patients 2-4. In patient 5, additional measures promoting recipient cell repopulation were made. The trachea may be used as a composite tissue allotransplant after heterotopic revascularization in the forearm. Measures to maximize recipient cell repopulation may be important in maintaining the viability of the transplant after cessation of immunosuppression.
Coloscopy: A Necessary Aid in the Diagnosis of Transient Ischemic Colitis (1978)

After a short review on endoscopic diagnosis of transient ischemic colitis, the author calls attention to the ischemic pathology of the colon emphasizing the importance of coloscopy as a necessary aid in the diagnosis of these diseases.
Traffic Signal Control System Based on Intelligent Transportation System and Reinforcement Learning (2021)

Traffic congestion has several causes, including insufficient road capacity, unrestricted demand and improper scheduling of traffic signal phases. A great variety of efforts have been made to properly program such phases. Some of them are based on traditional transportation assumptions, and others are adaptive, allowing the system to learn the control law (signal program) from data obtained from different sources. Reinforcement Learning (RL) is a technique commonly used in previous research. However, properly determining the states and the reward is key to obtain good results and to have a real chance to implement it. This paper proposes and implements a traffic signal control system (TSCS), detailing its development stages: (a) Intelligent Transportation System (ITS) architecture design for the TSCS; (b) design and development of a system prototype, including an RL algorithm to minimize the vehicle queue at intersections, and detection and calculation of such queues by adapting a computer vision algorithm; and (c) design and development of system tests to validate operation of the algorithms and the system prototype. Results include the development of the tests for each module (vehicle queue measurement and RL algorithm) and real-time integration tests. Finally, the article presents a system simulation in the context of a medium-sized city in a developing country, showing that the proposed system allowed reduction of vehicle queues by 29%, of waiting time by 50%, and of lost time by 50%, when compared to fixed phase times in traffic signals.
Regulation of Substrate Recognition by the MiaA tRNA Prenyltransferase Modification Enzyme of Escherichia coliK-12 (1997)

We purified polyhistidine (His6)-tagged and native Escherichia coli MiaA tRNA prenyltransferase, which uses dimethylallyl diphosphate (DMAPP) to isopentenylate A residues adjacent to the anticodons of most tRNA species that read codons starting with U residues. Kinetic and binding studies of purified MiaA were performed with several substrates, including synthetic wild-type tRNAPhe, the anticodon stem-loop (ACSLPhe) of tRNAPhe, and bulk tRNA isolated from a miaA mutant. Gel filtration shift and steady-state kinetic determinations showed that affinity-purified MiaA had the same properties as native MiaA and was completely active for tRNAPhe binding. MiaA had a Kmapp (tRNA substrates) approximately 3 nM, which is orders of magnitude lower than that of other purified tRNA modification enzymes, a Kmapp (DMAPP) = 632 nM, and a kcatapp = 0.44 s-1. MiaA activity was minimally affected by other modifications or nonsubstrate tRNA species present in bulk tRNA isolated from a miaA mutant. MiaA modified ACSLPhe with a kcatapp/Kmapp substrate specificity about 17-fold lower than that for intact tRNAPhe, mostly due to a decrease in apparent substrate binding affinity. Quantitative Western immunoblotting showed that MiaA is an abundant protein in exponentially growing bacteria (660 monomers per cell; 1.0 microM concentration) and is present in a catalytic excess. However, MiaA activity was strongly competitively inhibited for DMAPP by ATP and ADP (Kiapp = 0.06 microM), suggesting that MiaA activity is inhibited substantially in vivo and that DMAPP may bind to a conserved P-loop motif in this class of prenyltransferases. Band shift, filter binding, and gel filtration shift experiments support a model in which MiaA tRNA substrates are recognized by binding tightly to MiaA multimers possibly in a positively cooperative way (Kdapp approximately 0.07 microM).
Monocular Depth Estimation Using Laplacian Pyramid-Based Depth Residuals (2021)

With a great success of the generative model via deep neural networks, monocular depth estimation has been actively studied by exploiting various encoder-decoder architectures. However, the decoding process in most previous methods, which repeats simple up-sampling operations, probably fails to fully utilize underlying properties of well-encoded features for monocular depth estimation. To resolve this problem, we propose a simple but effective scheme by incorporating the Laplacian pyramid into the decoder architecture. Specifically, encoded features are fed into different streams for decoding depth residuals, which are defined by decomposition of the Laplacian pyramid, and corresponding outputs are progressively combined to reconstruct the final depth map from coarse to fine scales. This is fairly desirable to precisely estimate the depth boundary as well as the global layout. We also propose to apply weight standardization to pre-activation convolution blocks of the decoder architecture, which gives a great help to improve the flow of gradients and thus makes optimization easier. Experimental results on benchmark datasets constructed under various indoor and outdoor environments demonstrate that the proposed method is effective for monocular depth estimation compared to state-of-the-art models. The code and model are publicly available at: | <uri xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">https://github.com/tjqansthd/LapDepth-release</uri> |.