gebhart/patrepeval huggingface dataset:
Outer Membrane Protein P6 of Nontypeable<i>Haemophilus influenzae</i>Is a Potent and Selective Inducer of Human Macrophage Proinflammatory Cytokines (2005)

Interactions of nontypeable Haemophilus influenzae (NTHI) with human macrophages contribute to the pathogenesis of NTHI-induced infection in humans. However, the immunologic mechanisms that initiate and perpetuate NTHI-mediated macrophage responses have not been well explored. Outer membrane protein (OMP) P6 is a conserved lipoprotein expressed by NTHI in vivo that possesses a Pam(3)Cys terminal motif, characteristic of immunoactive bacterial lipoproteins associated with Toll-like receptor signaling. We theorized that OMP P6 is a potent immunomodulator of human macrophages. To test this hypothesis, we purified OMP P6 as well as OMP P2, the predominant NTHI outer membrane protein, and lipooligosaccharide (LOS), the specific endotoxin of NTHI, from NTHI strain 1479. Human blood monocyte-derived macrophages, purified from healthy donors, were incubated with each outer membrane constituent, and cytokine production of macrophage supernatants interleukin-1beta (IL-1beta), tumor necrosis factor alpha (TNF-alpha), IL-10, IL-12, and IL-8 was measured. OMP P6 selectively upregulated IL-10, TNF-alpha, and IL-8. While OMP P6 (0.1 mug/ml for 8 h) elicited slightly greater concentrations of IL-10, it resulted in over ninefold greater concentrations of TNF-alpha and over fourfold greater concentrations of IL-8 than did OMP P2. OMP P6 at doses as low as 10 pg/ml was still effective at induction of macrophage IL-8, while OMP P2 and LOS were not. OMP P6 of NTHI is a specific trigger of bacteria-induced human macrophage inflammatory events, with IL-8 and TNF-alpha as key effectors of P6-induced macrophage responses.
High-throughput Saccharification Assay for Lignocellulosic Materials (2011)

Polysaccharides that make up plant lignocellulosic biomass can be broken down to produce a range of sugars that subsequently can be used in establishing a biorefinery. These raw materials would constitute a new industrial platform, which is both sustainable and carbon neutral, to replace the current dependency on fossil fuel. The recalcitrance to deconstruction observed in lignocellulosic materials is produced by several intrinsic properties of plant cell walls. Crystalline cellulose is embedded in matrix polysaccharides such as xylans and arabinoxylans, and the whole structure is encased by the phenolic polymer lignin, that is also difficult to digest (1). In order to improve the digestibility of plant materials we need to discover the main bottlenecks for the saccharification of cell walls and also screen mutant and breeding populations to evaluate the variability in saccharification (2). These tasks require a high throughput approach and here we present an analytical platform that can perform saccharification analysis in a 96-well plate format. This platform has been developed to allow the screening of lignocellulose digestibility of large populations from varied plant species. We have scaled down the reaction volumes for gentle pretreatment, partial enzymatic hydrolysis and sugar determination, to allow large numbers to be assessed rapidly in an automated system. This automated platform works with milligram amounts of biomass, performing ball milling under controlled conditions to reduce the plant materials to a standardised particle size in a reproducible manner. Once the samples are ground, the automated formatting robot dispenses specified and recorded amounts of material into the corresponding wells of 96 deep well plate (Figure 1). Normally, we dispense the same material into 4 wells to have 4 replicates for analysis. Once the plates are filled with the plant material in the desired layout, they are manually moved to a liquid handling station (Figure 2). In this station the samples are subjected to a mild pretreatment with either dilute acid or alkaline and incubated at temperatures of up to 90°C. The pretreatment solution is subsequently removed and the samples are rinsed with buffer to return them to a suitable pH for hydrolysis. The samples are then incubated with an enzyme mixture for a variable length of time at 50°C. An aliquot is taken from the hydrolyzate and the reducing sugars are automatically determined by the MBTH colorimetric method.
Visual Languages: A Tutorial and Survey (1987)

Research into visual and growing in popularity. The concept of generalized icons is a framework for the design of the next genertion of visual languages.
<title>Design and testing of polysilicon surface-micromachined piston micromirror arrays</title> (1998)

THis paper presents optical testing of polysilicon surface micromachined piston micromirror arrays. Similar piston micromirror arrays were fabricated using two different commercially available surface micromachining foundry processes: the DARPA supported multi-user MEMS processes (MUMPs), and Sandia Ultra-planar Multi-level MEMS Technology (SUMMiT). All test arrays employ square reflecting elements in an 8 X 8 element 203 micrometers square grid. Fabrication constraints limit the MUMPs designs to fill-factors of less than 80 percent. The chemical mechanical polishing planarization step integral to the SUMMiT process allows an as-drawn fill-factor of 95 percent to be easily achieved. MUMPs designs employ both the standard gold metallization and maskless sputtered chromium/gold post-process metallization, while post process metallization is the only option for the SUMMiT design. Testing of the micromirror arrays focuses on microscope interferometer characterization of mirror topography, and measurement of the far field diffraction pattern for each. The measured results show that control of the individual micromirror element surface topography is more important for imaging applications than maximizing the as-drawn fill-factor.
Coarse symbol synchronization algorithms for OFDM systems in multipath channels (2002)

A maximum-likelihood (ML) coarse symbol synchronization algorithm for OFDM systems in multipath channels is derived. Several simplifying assumptions reduce the rather impractical ML algorithm to more practical forms of minimum mean-squared-error and maximum correlation algorithms. All of the derived algorithms exploit the cyclic prefix and thus do not require a known training sequence. Estimating the position of the first arriving path, the algorithms allow maximum utilization of the guard interval thus minimizing intersymbol interference.
Controlled drug release of silicone‐based adhesive‐containing cross‐linked siloxane powders as a reservoir (2015)

ABSTRACT We prepared pressure‐sensitive adhesive (PSA)‐containing cross‐linked siloxane powders (CS) as a reservoir for a transdermal drug delivery system (TDDS) and evaluated their sustained drug‐release properties. PSA, as a patch‐type adhesive, was synthesized by a hydrosilylation reaction of vinyl‐terminated polysiloxanes with hydrogen‐terminated polydimethylsiloxanes. CS was also prepared via a hydrosilylation process with vinyl‐terminated polydimethylsiloxane, 1,3,5,7‐tetramethyl‐1,3,5,7‐tetravinyltetracyclosiloxane ( ), hydrogen‐terminated polydimethylsiloxane, and dimethylhydrogenmethyl oligomeric siloxane copolymer. The results of release performances using ascorbic acid as a model drug showed a cumulative linear slope over a week, indicating a constant release performance. Our data suggest that this siloxane TDDS could be useful for constant drug release over a long period. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015 , 132 , 42154.
Real-time Cherenkov emission portal imaging during CyberKnife<sup>®</sup>radiotherapy (2015)

The feasibility of real-time portal imaging during radiation therapy, through the Cherenkov emission (CE) effect is investigated via a medical linear accelerator (CyberKnife(®)) irradiating a partially-filled water tank with a 60 mm circular beam. A graticule of lead/plywood and a number of tissue equivalent materials were alternatively placed at the beam entrance face while the induced CE at the exit face was imaged using a gated electron-multiplying-intensified-charged-coupled device (emICCD) for both stationary and dynamic scenarios. This was replicated on an Elekta Synergy(®) linear accelerator with portal images acquired using the iViewGT(™) system. Profiles across the acquired portal images were analysed to reveal the potential resolution and contrast limits of this novel CE based portal imaging technique and compared against the current standard. The CE resolution study revealed that using the lead/plywood graticule, separations down to 3.4 ± 0.5 mm can be resolved. A 28 mm thick tissue-equivalent rod with electron density of 1.69 relative to water demonstrated a CE contrast of 15% through air and 14% through water sections, as compared to a corresponding contrast of 19% and 12% using the iViewGT(™) system. For dynamic scenarios, video rate imaging with 30 frames per second was achieved. It is demonstrated that CE-based portal imaging is feasible to identify both stationary and dynamic objects within a CyberKnife(®) radiotherapy treatment field.
HydraPlus-Net: Attentive Deep Features for Pedestrian Analysis (2017)

Pedestrian analysis plays a vital role in intelligent video surveillance and is a key component for security-centric computer vision systems. Despite that the convolutional neural networks are remarkable in learning discriminative features from images, the learning of comprehensive features of pedestrians for fine-grained tasks remains an open problem. In this study, we propose a new attention-based deep neural network, named as HydraPlus-Net (HP-net), that multi-directionally feeds the multi-level attention maps to different feature layers. The attentive deep features learned from the proposed HP-net bring unique advantages: (1) the model is capable of capturing multiple attentions from low-level to semantic-level, and (2) it explores the multi-scale selectiveness of attentive features to enrich the final feature representations for a pedestrian image. We demonstrate the effectiveness and generality of the proposed HP-net for pedestrian analysis on two tasks, i.e. pedestrian attribute recognition and person re-identification. Intensive experimental results have been provided to prove that the HP-net outperforms the state-of-the-art methods on various datasets.
Performance of Temperature-Phased Anaerobic Digestion (TPAD) System Treating Dairy Cattle Wastes (2001)

ABSTRACT

The performance of Temperature-Phased Anaerobic Digestion (TPAD) system in the stabilization of dairy cattle wastes at high solids concentrations has never been evaluated, though the process has been established as a feasible alternative to conventional mesophilic processes for the treatment of municipal wastewater sludges. The TPAD system, operating at a retention time of 14 days, was subjected to varying total solids (3.46 – 14.54%) and volatile solids (2.62 – 10.78%) concentrations of dairy cattle wastes. At total solids concentrations less than 10.35% corresponding to a system volatile solids loading of 5.82 g VS/L/d, the system achieved VS removals in the range of 37.8 – 42.6%. The maximum VS destruction of 42.6% was achieved at a total solids concentration of 10.35%. There was a drop in the system performance with respect to VS removal and methane recovery at total solids concentrations higher than 10.35%. For all total solids concentrations studied, the indicator organism counts in the biosolids were within the limits specified by U.S. EPA in 40 CFR Part 503 regulations for Class A designation.
<i>SCN2A</i> encephalopathy (2015)

Review of our 12 and 34 other reported cases of SCN2A encephalopathy suggests 3 phenotypes: neonatal-infantile-onset groups with severe and intermediate outcomes, and a childhood-onset group. Here, we show that SCN2A is the second most common cause of EIMFS and, importantly, does not always have a poor developmental outcome. Sodium channel blockers, particularly phenytoin, may improve seizure control.
Which Reference Should We Use for EEG and ERP practice? (2019)

Which reference is appropriate for the scalp ERP and EEG studies? This unsettled problem still inspires unceasing debate. The ideal reference should be the one with zero or constant potential but unfortunately it is well known that no point on the body fulfills this condition. Consequently, more than ten references are used in the present EEG-ERP studies. This diversity seriously undermines the reproducibility and comparability of results across laboratories. A comprehensive review accompanied by a brief communication with rigorous derivations and notable properties (Hu et al. Brain Topogr, 2019. https://doi.org/10.1007/s10548-019-00706-y ) is thus necessary to provide application-oriented principled recommendations. In this paper current popular references are classified into two categories: (1) unipolar references that construct a neutral reference, including both online unipolar references and offline re-references. Examples of unipolar references are the reference electrode standardization technique (REST), average reference (AR), and linked-mastoids/ears reference (LM); (2) non-unipolar references that include the bipolar reference and the Laplacian reference. We show that each reference is derived with a different assumption and serves different aims. We also note from (Hu et al. 2019) that there is a general form for the reference problem, the 'no memory' property of the unipolar references, and a unified estimator for the potentials at infinity termed as the regularized REST (rREST) which has more advantageous statistical evidence than AR. A thorough discussion of the advantages and limitations of references is provided with recommendations in the hope to clarify the role of each reference in the ERP and EEG practice.
Platform technology for scalable assembly of instantaneously functional mosaic tissues (2015)

Engineering mature tissues requires a guided assembly of cells into organized three-dimensional (3D) structures with multiple cell types. Guidance is usually achieved by microtopographical scaffold cues or by cell-gel compaction. The assembly of individual units into functional 3D tissues is often time-consuming, relying on cell ingrowth and matrix remodeling, whereas disassembly requires an invasive method that includes either matrix dissolution or mechanical cutting. We invented Tissue-Velcro, a bio-scaffold with a microfabricated hook and loop system. The assembly of Tissue-Velcro preserved the guided cell alignment realized by the topographical features in the 2D scaffold mesh and allowed for the instant establishment of coculture conditions by spatially defined stacking of cardiac cell layers or through endothelial cell coating. The assembled cardiac 3D tissue constructs were immediately functional as measured by their ability to contract in response to electrical field stimulation. Facile, on-demand tissue disassembly was demonstrated while preserving the structure, physical integrity, and beating function of individual layers.
Optimal Orthogonal Precoding for Power Leakage Suppression in DFT-Based Systems (2010)

A solution to the power leakage minimization problem in discrete Fourier transform (DFT) based communication systems is presented. In a conventional DFT based system, modulated subcarriers exhibit high sidelobe levels, which leads to significant out-of-band power leakage. Existing techniques found in the literature either do not achieve sufficient sidelobe suppression or suffer from significant spectral efficiency loss. Precoding can be seen as a general linear processing method for power leakage reduction, however, how to design the optimal linear precoder is still an open problem. In this paper, the power leakage suppression is first treated as a matrix Frobenius norm minimization problem, and then the optimal orthogonal precoding matrix design for the power leakage suppression is proposed based on singular value decomposition (SVD). By further exploiting the extra degrees of freedom in the precoding matrix, two kinds of optimized precoding matrices, one with multi-carrier property and the other with single-carrier property, are developed to take the advantages of orthogonal frequency division multiplexing (OFDM) and single carrier frequency division multiple access (SC-FDMA), respectively. Simulation results show that both the multi-carrier and the single-carrier precoding schemes achieve significant power leakage suppression, and have similar peak-to-average power ratio (PAPR) and bit-error-rate (BER) to those of OFDM and SC-FDMA systems, respectively.
Synthesis of Highly Substituted Pyridazines through Alkynyl Boronic Ester Cycloaddition Reactions (2005)

A highly regioselective transformation of tetrazines through a cycloaddition reaction with alkynyl boronic esters provides highly substituted pyridazine boronic esters as intermediates for CO and CC bond-forming reactions (see scheme). Functionalization reactions of the CB bond, such as oxidation and the Suzuki cross-coupling, show the versatility of these species.
Mashup-based information retrieval for domain experts (2009)

In this paper, we tackle the problem of helping domain experts to construct, parameterize and deploy mashups of data and code. We view a mashup as a data processing flow, that describes how data is obtained from one or more sources, processed by one or more components, and finally sent to one or more sinks. Our approach allows specifying patterns of flows, in a language called Cascade. The patterns cover different possible variations of the flows, including variations in the structure of the flow, the components in the flow and the possible parameterizations of these components. We present a tool that makes use of this knowledge of flow patterns and associated metadata to allow domain experts to explore the space of possible flows described in the pattern. The tool uses an AI planning approach to automatically build a flow, belonging to the flow pattern, from a high-level goal, specified as a set of tags. We describe examples from the financial services domain to show the use of flow patterns in allowing domain experts to construct a large variety of mashups rapidly.
Study on the preparation and electric conductivity of 3,4-ethylenedioxy thiophene modified silk (2010)

3,4-ethylenedioxy thiophene modified silk was prepared.The effects of monomer concentration,reaction time,temperature and pH value on silk modification were investigated.The optimal modification technology was obtained through investigation of effect of experiment factors on silk weight,volume resistivity(0.3 mol/L of monomer,pH 6r,eacted at 70 ℃ for 12 h).The electric conductivity and structure of modified silk were measured.The results showed that 3,4-ethylenedioxy thiophene treated silk had good electric conductivity.
Decision Support for Small Players Negotiations Under a Transactive Energy Framework (2018)

This paper proposes a decision support model to optimize small players' negotiations in multiple alternative/complementary market opportunities. The proposed model endows players with the ability to maximize their gains in electricity market negotiations. The proposed approach is integrated in a multi-agent simulation platform, which enables experimenting different market configurations, thus facilitating the assessment of the impact of negotiation outcomes in distinct electricity markets. The proposed model is directed to supporting the actions of small players in a transactive energy environment. Therefore, the experimental findings include negotiations in local markets, negotiations through bilateral contracts, and the participation in wholesale markets (through aggregators). The validation is performed using real data from the Iberian market, and results show that by planning market actions considering the expected prices in different market opportunities, small players are able to improve their benefits from market negotiations.
Behavioral Modeling: an Overview (2008)

This exploratory paper begins with an overview of a multidisciplinary problem of behavior modeling and correlation of different behaviors. It looks at many possible applications of such technology and proposes some novel directions for future research. From the security point of view the paper proposes and explores some novel behavioral biometrics and research paths as well as some universal descriptors of behavior in general. It concludes with an analysis of how behavior can be influenced by the environment in particular location of the individual engaging in the behavior.
Pulsed plasma propulsion system for North-South stationkeeping (1976)

A completely integrated pulsed plasma propulsion system for North-South stationkeeping has been developed. System testing on a thrust balance has provided following new results: (1) A simple, helically coiled, spring-fed propellant subsystem for 38,284 lb-s (166,000 N-S) total impulse is feasible; (2) The propulsive performance level is compatible with North-South stationkeeping requirements; (3) Power conditioning is as simple as that of the space flight proven microthruster system; (4) Vacuum compatible, high energy density capacitors (40 joules/lb) capacitors have been developed and tested as part of the system; (5) 10,000 lb-sec of total impulse has been generated. Future improvements will include reducing electrode erosion, improving the structural rigidity of the assembly and continuously operating the system until 37,000 lb-sec of total impulse have been generated.
Production of enterotoxin by Yersinia enterocolitica (1978)

Forty-three strains of Yersinia enterocolitica isolated from children with gastroenteritis and 18 laboratory strains were examined for enterotoxin production by using the infant mouse, Y1 adrenal cell, and rabbit ileal loop assay systems. All clinical isolates and seven laboratory strains were found enterotoxigenic in the infant mouse model, but none of the strains produced toxin in the Y1 adrenal cell system. One enterotoxin-producing strain was also tested by the rabbit ileal loop assay, confirming the results of the infant mouse assay. The enterotoxin was heat-stable and as active in the 6-h rabbit ileal loop as in the 18-h assay, suggesting a similarity to the heat-stable enterotoxin of Escherichia coli.
Monitor and understand pilgrims (2013)

Each year, millions of people visit the sacred sites in Makkah and Madinah. Even though the Hajj pilgrimage is one of the biggest annual events in the world, with many of the pilgrims reporting it as a life-changing experience, quite a little is done to objectively monitor the pilgrims and to understand from the crowd and from the individual point of view what makes this event so special. We present a data collection phase of 8 days of pilgrimage in April 2013 with 41 pilgrims carrying Android smartphones and 10 pilgrims wearing two physiological sensors, namely chest belts and wrist-worn devices. We describe the data recording itself, and emphasize the problems raised and the challenges faced during the study. We provide the best practices for performing solid and efficient user studies in such a difficult environment, and give first insights towards measuring important aspects of the Hajj pilgrimage such as recognition of activities and stages, analysis of group behavior, detection of stressful situations and health monitoring of pilgrims in general.