gebhart/patrepeval huggingface dataset:
Self-aligned gated field emission devices using single carbon nanofiber cathodes (2002)

We report on the fabrication and operation of integrated gated field emission devices using single vertically aligned carbon nanofiber (VACNF) cathodes where the gate aperture has been formed using a self-aligned technique based on chemical mechanical polishing. We find that this method for producing gated cathode devices easily achieves structures with gate apertures on the order of 2 μm that show good concentric alignment to the VACNF emitter. The operation of these devices was explored and field emission characteristics that fit well to the Fowler–Nordheim model of emission was demonstrated.
Preparation of large-particle-size monodisperse polystyrene latexes in microgravity (1982)

Three large-particle-size monodisperse latexes (3.44, 4.08, and 4.98 micron diameter) were prepared in an automated four-reactor apparatus on the third orbital mission of the 'Columbia' begun on March 22. Comparison with ground-based controls showed that the 4.98 micron-size flight sample was the more uniform; the uniformity at the other two sizes was about the same. The rates of polymerization in microgravity and on earth were the same within experimental error, demonstrating that radical-initiated vinyl addition polymerizations are unaffected by the weightless environment.
Conjugated Linoleic Acid (CLA) Content of Milk from Cows Offered Diets Rich in Linoleic and Linolenic Acid (2000)

Two experiments were conducted to determine the conjugated linoleic acid (CLA) content of milk from cows offered diets rich in linoleic and linolenic acid. In experiment 1, 36 cows were assigned to a control and five treatment groups. Cows in the control group received a diet containing 51% forage and 49% grain on a dry matter basis. In the treatment group, grain was partly replaced by either 18% raw cracked soybeans, 18% roasted cracked soybeans, 3.6% soybean oil, 2.2% linseed oil, or 4.4% linseed oil. Experimental diets were fed for 5 wk. Average CLA contents in milk fat from wk 2 through 5 were 0.39% in control and 0.37, 0.77, 2.10, 1.58, and 1.63% of total fatty acids in the raw soybean, roasted soybeans, soybean oil, 2.2% linseed oil, and 4.4% linseed oil treatments, respectively. In experiment 2, 36 cows were assigned to a control and 5 treatment groups. Cows in the control group received a diet containing 55% forage and 45% grain. In the treatment groups, grain was partly replaced by soybean oil at 0.5, 1.0, 2.0, 4.0, or by linseed oil at 1.0% of the dietary dry matter. Experimental diets were fed for 5 wk. Average CLA contents in milk fat from wk 2 through 5 were 0.50% in control and 0.75, 0.76, 1.45, 2.08, and 0.73% of total fatty acids in 0.5, 1.0, 2.0, 4.0 soybean oil and 1.0% linseed oil treatments, respectively. Diets rich in linoleic or linolenic acid can increase CLA content of milk when dietary oil is accessible to the rumen microorganisms.
Imaging of Flow through the Nasal Cavity and Paranasal Sinuses: Preliminary Work with Hyperpolarized Xenon (1999)

Experience has been gained at several imaging sites with the use of hyperpolarized gases to image the lungs and the proximal airways. The theoretical advantage to using a polarized noble gas is the dramatically higher polarization values when compared with the small fraction of the hydrogen spin polarization currently utilized in magnetic resonance imaging. Using polarized noble gases, the potential increased signal enhancement is on the order of 100 fold. Hyperpolarized gases provide the ability to image air flow and air-containing structures. Utilization of specially tuned coils on standard clinical imaging systems has enabled imaging of the lungs and airways, using the hyperpolarized gas as a positive contrast agent. We have begun work on imaging the real-time air flow through the nasal cavity and paranasal sinuses, about which little is currently known. Rapid acquisition techniques allow us to perform dynamic imaging of the pattern of airflow through the nasal cavity, and gain information regarding the patterns of air exchange within the paranasal sinuses. This technique may lead to understanding of air flow in normal and disease states.
High-Resolution Transfer Printing of Graphene Lines for Fully Printed, Flexible Electronics (2017)

Pristine graphene inks show great promise for flexible printed electronics due to their high electrical conductivity and robust mechanical, chemical, and environmental stability. While traditional liquid-phase printing methods can produce graphene patterns with a resolution of ∼30 μm, more precise techniques are required for improved device performance and integration density. A high-resolution transfer printing method is developed here capable of printing conductive graphene patterns on plastic with line width and spacing as small as 3.2 and 1 μm, respectively. The core of this method lies in the design of a graphene ink and its integration with a thermally robust mold that enables annealing at up to ∼250 °C for precise, high-performance graphene patterns. These patterns exhibit excellent electrical and mechanical properties, enabling favorable operation as electrodes in fully printed electrolyte-gated transistors and inverters with stable performance even following cyclic bending to a strain of 1%. The high resolution coupled with excellent control over the line edge roughness to below 25 nm enables aggressive scaling of transistor dimensions, offering a compelling route for the scalable manufacturing of flexible nanoelectronic devices.
Hall sensor array (2010)

Patent number: EP10168375 Reference EPFL-PATENT-163427Search for this record at the European Patent Office Record created on 2011-02-15, modified on 2016-08-09
Functional map of arrestin-1 at single amino acid resolution (2014)

Arrestins function as adapter proteins that mediate G protein-coupled receptor (GPCR) desensitization, internalization, and additional rounds of signaling. Here we have compared binding of the GPCR rhodopsin to 403 mutants of arrestin-1 covering its complete sequence. This comprehensive and unbiased mutagenesis approach provides a functional dimension to the crystal structures of inactive, preactivated p44 and phosphopeptide-bound arrestins and will guide our understanding of arrestin-GPCR complexes. The presented functional map quantitatively connects critical interactions in the polar core and along the C tail of arrestin. A series of amino acids (Phe375, Phe377, Phe380, and Arg382) anchor the C tail in a position that blocks binding of the receptor. Interaction of phosphates in the rhodopsin C terminus with Arg29 controls a C-tail exchange mechanism in which the C tail of arrestin is released and exposes several charged amino acids (Lys14, Lys15, Arg18, Lys20, Lys110, and Lys300) for binding of the phosphorylated receptor C terminus. In addition to this arrestin phosphosensor, our data reveal several patches of amino acids in the finger (Gln69 and Asp73-Met75) and the lariat loops (L249-S252 and Y254) that can act as direct binding interfaces. A stretch of amino acids at the edge of the C domain (Trp194-Ser199, Gly337-Gly340, Thr343, and Thr345) could act as membrane anchor, binding interface for a second rhodopsin, or rearrange closer to the central loops upon complex formation. We discuss these interfaces in the context of experimentally guided docking between the crystal structures of arrestin and light-activated rhodopsin.
Decoding of Code-Multiplexed Coulter Sensor Signals via Deep Learning (2019)

Code-multiplexed Coulter sensors can easily be integrated into microfluidic devices and provide information on spatiotemporal manipulations of suspended particles for quantitative sample assessment. In this paper, we introduced a deep learning-based decoding algorithm to process the output waveform from a network of code-multiplexed Coulter sensors on a microfluidic device. Our deep learning-based algorithm both simplifies the design of coded Coulter sensors and increases the signal processing speed. As a proof of principle, we designed and fabricated a microfluidic platform with 10 code-multiplexed Coulter sensors, and used a suspension of human ovarian cancer cells as a test sample to characterize the system. Our deep learning-based algorithm resulted in an 87% decoding accuracy at a sample processing speed of 800 particles/s.
Defect-tolerant demultiplexer circuits based on threshold logic and coding (2009)

A defect-tolerant design is presented for a demultiplexer circuit that is based on threshold logic. The design uses coding both to handle (i.e., tolerate) defects in the circuit and to improve the voltage margin in its gates. The following model is assumed for the defects: configured junctions can become either stuck open or stuck closed, and non-configured junctions can become shorted. Two realizations of the circuit are presented: one using conventional transistor circuitry, and the other using nanoscale components and wiring. The design presented in this paper demonstrates how a standard digital building-block circuit-a demultiplexer-can be efficiently protected against several types of defect simultaneously.
Miniature Housings for Electronics With Standard Interfaces (2006)

A family of general-purpose miniature housings has been designed to contain diverse sensors, actuators, and drive circuits plus associated digital electronic readout and control circuits. The circuits contained in the housings communicate with the external world via standard RS-485 interfaces.
MB-05 * PHARMACOLOGICAL INHIBITION OF MRK/ZAK KINASE FOR THE TREATMENT OF MEDULLOBLASTOMA (2015)

Medulloblastoma is a cerebellar tumor and the most common pediatric brain malignancy. Radiation therapy is part of the standard care for this tumor, but its effectiveness is accompanied by significant neurocognitive sequelae due to the deleterious effects of radiation on the developing brain. We have previously shown that the protein kinase MRK/ZAK protects tumor cells from radiation-induced cell death by regulating cell cycle arrest after ionizing radiation. Here, we show that siRNA-mediated MRK depletion sensitizes medulloblastoma primary cells to radiation. We have, therefore, designed and tested a small molecule inhibitor of MRK, M443, which binds to MRK in an irreversible fashion and inhibits its activity. We found that M443 strongly radio-sensitizes UW228 medulloblastoma cells as well as IMB226 patient-derived primary cells. M443 also inhibits radiation-induced activation of both p38 and Chk2, two proteins that act downstream of MRK and are involved in DNA damage-induced cell cycle arrest. We also tested the effect of M443 in an animal model of medulloblastoma that employs orthotopic implantation of IMB226 medulloblastoma cells in nude mice. Intra-tumoral delivery of M443 alone significantly extended animal survival by 5 days compared to vehicle treatment. Combination treatment of M443 with radiation at 2 x 3 Gy, that is not effective on its own (1 day of additional survival over control), achieved a synergistic increase in survival (15 days over the control survival time). Western blotting of tumor lysates demonstrated strong inhibition of MRK activity. In conclusion, we have developed a new small molecule inhibitor of MRK/ZAK that radio-sensitizes medulloblastoma cells. We hypothesize that combining radio-therapy with M443 will allow us to lower the radiation dose while maintaining therapeutic efficacy, thereby minimizing radiation-induced side effects.
ChemInform Abstract: AN IMPROVED AND CONVENIENT PROCEDURE FOR THE SYNTHESIS OF 1‐SUBSTITUTED IMIDAZOLES (1983)

Abstract Durch Acylierung von Imidazol (I), nachfolgende Alkylierung und Abspaltung des Acyl‐Restes aus den gebildeten 1‐Acyl‐3‐alkyl‐imidazolen (III) werden in guten Ausbeuten die I‐Alkylimidazole (IV) hergestellt.
Structural Characterization of a Minimal Antibody against Human APOBEC3B (2021)

APOBEC3B (A3B) is one of seven human APOBEC3 DNA cytosine deaminases that restrict viral infections as part of the overall innate immune response, but it also plays a major role in tumor evolution by mutating genomic DNA. Given the importance of A3B as a restriction factor of viral infections and as a driver of multiple human cancers, selective antibodies against A3B are highly desirable for its specific detection in various research and possibly diagnostic applications. Here, we describe a high-affinity minimal antibody, designated 5G7, obtained via a phage display screening against the C-terminal catalytic domain (ctd) of A3B. 5G7 also binds APOBEC3A that is highly homologous to A3Bctd but does not bind the catalytic domain of APOBEC3G, another Z1-type deaminase domain. The crystal structure of 5G7 shows a canonical arrangement of the heavy and light chain variable domains, with their complementarity-determining region (CDR) loops lining an antigen-binding cleft that accommodates a pair of α-helices. To understand the mechanism of A3Bctd recognition by 5G7, we used the crystal structures of A3Bctd and 5G7 as templates and computationally predicted the A3B-5G7 complex structure. Stable binding poses obtained by the simulation were further tested by site-directed mutagenesis and in vitro binding analyses. These studies mapped the epitope for 5G7 to a portion of C-terminal α6 helix of A3Bctd, with Arg374 playing an essential role. The same region of A3Bctd was used previously as a peptide antigen for generating a rabbit monoclonal antibody (mAb 5210-87-13), suggesting that this region is particularly immunogenic and that these antibodies from very different origins may share similar binding modes. Our studies provide a platform for the development of selective antibodies against A3B and other APOBEC3 family enzymes.
Air Separation Unit Integration for Alternative Fuel Projects (1998)

Alternative fuel projects often require substantial amounts of oxygen. World scale gas-to-liquids (GTL) processes based on the partial oxidation of natural gas, followed by Fischer-Tropsch chemistry and product upgrading, may require in excess of 10,000 tons per day of pressurized oxygen. The remote location of many of these proposed projects and the availability of low-cost natural gas and byproduct steam from the GTL process disadvantages the use of traditional, motor-driven air separation units in favor of steam or gas turbine drive facilities. Another process of current interest is the partial oxidation of waste materials in industrial areas to generate synthesis gas. Synthesis gas may be processed into fuels and chemicals, or combusted in gas turbines to produce electricity. A key to the economic viability of such oxygen-based processes is cost effective air separation units, and the manner in which they are integrated with the rest of the facility. Because the trade-off between capital and energy is different for the remote gas and the industrial locations, the optimum integration schemes can also differ significantly. This paper examines various methods of integrating unit operations to improve the economics of alternative fuel facilities. Integration concepts include heat recovery, as well as several uses of byproduct nitrogen to enhance gas turbine operation or power production. Start-up, control and operational aspects are presented to complete the review of integrated designs.
Dual cavity optical automatic gain control for EDFAs (2003)

We believe we report the first demonstration of dual-cavity optical automatic gain control (OAGC). The dual-cavity technique results in improved stability of the amplifier gain spectrum under pump and signal power variations as compared to conventional OAGC.
End-to-End Neural Network Based Automated Speech Scoring (2018)

In recent years, machine learning models for automated speech scoring systems were mainly built using data-driven approaches with handcrafted features as one of the main components. However, the remarkable successes of deep learning (DL) technology in a variety of machine learning tasks has demonstrated its effectiveness in extracting features. Although there have been some efforts in utilizing DL technology for the automated speech scoring task, a thorough investigation of learning useful features is still missing. In this paper, we propose an end-to-end solution that consists of using deep neural network models to encode both lexical and acoustical cues to learn predictive features automatically. Experiments also confirm the effectiveness of our proposed solution compared to conventional methods based on handcrafted features.
A Dual-Source Approach for 3D Human Pose Estimation from a Single Image (2017)

In this work we address the challenging problem of 3D human pose estimation from single images. Recent approaches learn deep neural networks to regress 3D pose directly from images. One major challenge for such methods, however, is the collection of training data. Specifically, collecting large amounts of training data containing unconstrained images annotated with accurate 3D poses is infeasible. We therefore propose to use two independent training sources. The first source consists of accurate 3D motion capture data, and the second source consists of unconstrained images with annotated 2D poses. To integrate both sources, we propose a dual-source approach that combines 2D pose estimation with efficient 3D pose retrieval. To this end, we first convert the motion capture data into a normalized 2D pose space, and separately learn a 2D pose estimation model from the image data. During inference, we estimate the 2D pose and efficiently retrieve the nearest 3D poses. We then jointly estimate a mapping from the 3D pose space to the image and reconstruct the 3D pose. We provide a comprehensive evaluation of the proposed method and experimentally demonstrate the effectiveness of our approach, even when the skeleton structures of the two sources differ substantially.
Detection of obstructive sleep apnea from cardiac interbeat interval time series (2002)

Presents a new automated method to diagnose and quantify obstructive sleep apnea from single-lead electrocardiograms based on the detection of the periodic oscillations in cardiac interbeat intervals that are often associated with prolonged cycles of sleep apnea. This technique employs the Hilbert transformation of the sinus interbeat interval time series to derive the instantaneous amplitudes and frequencies of the series and calculates their averages and standard deviations over a moving 5-minute window. The authors then apply a thresholding technique and detect continuous sequences of those windows that lie within threshold limits. When applied to the Computers in Cardiology sleep apnea test data, the authors' algorithm correctly classified 28 out of 30 cases (93.3%) of both sleep apnea and normal subjects, and correctly identified the presence or absence of sleep apnea in 14,591 out of a total of 17,268 minutes (84.5%) of the data from the test set.
Blocked Ras Activation in Anergic CD4 <sup>+</sup> T Cells (1996)

T cell anergy is a state of functional unresponsiveness characterized by the inability to produce interleukin-2 (IL-2) upon T cell receptor stimulation. The mitogen-activated protein kinases ERK-1 and ERK-2 and the guanosine triphosphate-binding protein p21ras were found to remain unactivated upon stimulation of anergic murine T helper cell 1 clones. The inability to activate the Ras pathway did not result from a defect in association among Shc, Grb-2, and murine Son of Sevenless, nor from a defect in their tyrosine phosphorylation. This block in Ras activation may lead to defective transactivation at activator protein 1 sites in anergic cells and may enable T cells to shut down IL-2 production selectively during anergy.
Differentiable parameterization of Catmull-Clark subdivision surfaces (2004)

Subdivision-based representations are recognized as important tools for the generation of high-quality surfaces for Computer Graphics. In this paper we describe two parameterizations of Catmull-Clark subdivision surfaces that allow a variety of algorithms designed for other types of parametric surfaces (i.e., B-splines) to be directly applied to subdivision surfaces. In contrast with the natural parameterization of subdivision surfaces characterized by diverging first order derivatives around extraordinary vertices of valence higher than four, the derivatives associated with our proposed methods are defined everywhere on the surface. This is especially important for Computer-Aided Design (CAD) applications that seek to address the limitations of NURBS-based representations through the more flexible subdivision framework.
A Four-Monoclonal Antibody Combination Potently Neutralizes Multiple Botulinum Neurotoxin Serotypes C and D (2021)

Human botulism can be caused by botulinum neurotoxin (BoNT) serotypes A to G. Here, we present an antibody-based antitoxin composed of four human monoclonal antibodies (mAbs) against BoNT/C, BoNT/D, and their mosaic toxins. This work built on our success in generating protective mAbs to BoNT /A, B and E serotypes. We generated mAbs from human immune single-chain Fv (scFv) yeast-display libraries and isolated scFvs with high affinity for BoNT/C, BoNT/CD, BoNT/DC and BoNT/D serotypes. We identified four mAbs that bound non-overlapping epitopes on multiple serotypes and mosaic BoNTs. Three of the mAbs underwent molecular evolution to increase affinity. A four-mAb combination provided high-affinity binding and BoNT neutralization of both serotypes and their mosaic toxins. The mAbs have potential utility as therapeutics and as diagnostics capable of recognizing and neutralizing BoNT/C and BoNT/D serotypes and their mosaic toxins. A derivative of the four-antibody combination (NTM-1634) completed a Phase 1 clinical trial (Snow et al., Antimicrobial Agents and Chemotherapy, 2019) with no drug-related serious adverse events.
Ultrasonic transducer for use in a vibratory environment (1984)

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Improving the electrical conductivity of polymer acid-doped polyaniline by controlling the template molecular weight (2007)

We report the synthesis and characterization of a series of polyanilines (PANI) that are templated with a polymer acid at varying molecular weights. The polymer acid template of choice, poly(2-acrylamido-2-methyl-1-propanesulfonic acid), or PAAMPSA, was synthesized via conventional free-radical polymerization with molecular weights ranging from 45 kg mol−1 to 724 kg mol−1. Subsequent aniline polymerization in the presence of PAAMPSA yielded water-dispersible, conductive PANI–PAAMPSA. The electrical conductivity of PANI–PAAMPSA increases by approximately three-fold with decreasing PAAMPSA molecular weight. This trend is accompanied by significant structural changes, including increases in PANI crystallinity and conjugation length. These conductivities (0.4–1.1 S cm−1) exceed the highest reported for polymer acid-doped PANI systems.
Detection of human B-lymphotropic virus (human herpesvirus 6) sequences in B cell lymphoma tissues of three patients. (1988)

A survey for HBLV (human B-lymphotropic herpesvirus or human herpesvirus 6) sequences by Southern blot analyses was performed on DNA obtained from a variety of pathologically defined tissues, including a number of lymphomas, leukemias, and tissues from other hematologic disorders. Of the over 50 specimens studied, viral sequences were detected in three lymphomas of B cell derivation: an Epstein-Barr virus-positive African Burkitt's lymphoma, a follicular large cell lymphoma (nodular histocytic lymphoma), and two Epstein-Barr virus-negative tumors from a patient with Sjogren's syndrome. These results are the first indication of HBLV sequences associated with B cell tumors in a limited number of cases and raise the possibility that the virus might be involved in the genesis of some B cell tumors.