gebhart/patrepeval huggingface dataset:
Efficient Delivery of Bcl-2-Targeted siRNA Using Cationic Polymer Nanoparticles: Downregulating mRNA Expression Level and Sensitizing Cancer Cells to Anticancer Drug (2008)

In this study, cationic nanoparticles self-assembled from the amphiphilic copolymer poly(N-methyldietheneamine sebacate)-co-[(cholesteryl oxocarbonylamido ethyl) methyl bis(ethylene) ammonium bromide] sebacate) (P(MDS-co-CES) were synthesized and used to deliver Bcl-2 targeted siRNA into HepG2, HeLa and MDA-MB-231 cell lines, and downregulate Bcl-2 mRNA expression levels. Confocal microscopic studies show that the nanoparticles were able to complex with siRNA and deliver it inside the cells efficiently, but siRNA was easily dissociated from the complexes in the cytoplasm for its biological functions. Bcl-2 mRNA expression levels as low as 10% were achieved after treatment with nanoparticle/siRNA complexes. The downregulation efficiency of Bcl-2 mRNA level was similar to that mediated by Lipofectamine but higher than that induced by PEI. PEG was also conjugated to siRNA via a cleavable disulfide bond, and nanoparticle/siRNA-PEG complexes showed no significant protein adsorption as compared with 26 and 17% for blank nanoparticles and nanoparticle/siRNA complexes, respectively. The presence of serum caused slight aggregation of nanoparticle/siRNA or nanoparticle/siRNA-PEG complexes. However, the size of the complexes was still below 250 nm after being incubated in PBS containing 10% serum for 4 h. On the other hand, PEGylated siRNA delivered by the nanoparticles downregulated Bcl-2 mRNA expression level in the cells as efficiently as unmodified siRNA. Bcl-2 protein was also downregulated efficiently by nanoparticle/siRNA complexes in all cell lines tested. The downregulation of Bcl-2 mRNA or Bcl-2 protein did not show significant cell death in the tested siRNA and polymer concentration range. However, the delivery of siRNA sensitized HeLa cells to paclitaxel treatment, yielding significant improvement over the untreated cells (p<0.05). These cationic nanoparticles may be potentially employed to downregulate Bcl-2 expression and sensitize cancer cells to anticancer drugs for more efficient chemotherapy.
Automating Product-Line Variant Selection for Mobile Devices (2007)

Product-line architectures (PLAs) designed for mobile devices create a unique challenge for automated product variant selection engines since variants must be derived on-the-fly as devices are discovered. Current automation techniques do not incorporate device resource consumption constraints into variant selection and do not address how a PLA can be designed to improve automated variant selection speed. This paper presents a tool called Scatter whose input is (1) the requirements of PLA construction and (2) the resources available on a discovered mobile device and whose output is the optimal variant that can be deployed to the device. Scatter provides automatic variant selection based on configuration and resource constraints and also ensures that variant selection is optimal with regard to a configurable cost function. The paper presents our results from experiments with Scatter and how PLA design decisions affect a constraint-based variant selection engine's solving speed.
Can spleen transplantation induce tolerance? A review of the literature (2003)

In some rodent strain combinations, allogeneic spleen transplantation induces tolerance spontaneously to itself and to other donor-specific organs. In other combinations, a state of tolerance has been achieved in the weakened immune system of the recipient. The data indicate that if a balance can be achieved between host-versus-graft and graft-versus-host responses, tolerance develops, possibly due to the development of suppressor/regulatory cells. There have been a number of unsuccessful studies in outbred large animals, but none in MHC-defined donor-recipient pairs, and none in which the protocol specifically aimed at inducing tolerance. Spleen transplantation has been performed in approximately 50 humans for a number of reasons, however no clear immunologic advantage has been reported. Graft-versus-host disease (GVHD) was documented in at least 3 patients, and was lethal in one case, despite excision of the donor spleen. The advantages of tolerance over chronic immunosuppressive therapy are so great that a potentially tolerogenic approach such as spleen transplantation would seem worthy of further investigation in a suitable large animal model. Such a study is ongoing at our center.
Approach for quality detection of food by RFID‐based wireless sensor tag (2013)

A novel wireless sensor for the detection of food quality is presented. The main idea is to transform radio frequency identification (RFID) tags into RFID sensors, owing to a specific design of the tag antenna. From knowledge of the variation of the permittivity of food over time through experimental characterisation, it is possible to detect the time from which the food becomes improper for consumption based on the read‐range measurement of the designed sensing tags. This low‐cost ultra‐high frequency (UHF) RFID passive sensor was designed and experimentally tested on plastic‐film‐wrapped beef meat. The overall agreement between the experimental and simulation results shows the potential of this technique for real‐world applications in food traceability.
Inflatable antenna for CubeSat: Extension of the previously developed S-Band design to the X-Band (2015)

The inflatable antenna for CubeSat is a 1 meter antenna reflector designed with one side reflective Mylar, another side clear Mylar with a patch antenna at the focus. The development of this technology responds to the increasing need for more capable communication systems to allow CubeSats to operate autonomously in interplanetary missions. An initial version of the antenna for the S-Band was developed and tested in both anechoic chamber and vacuum chamber. Recent developments in transceivers and amplifiers for CubeSat at X-band motivated the extension from the S-Band to the X-Band. This paper describes the process of extending the design of the antenna to the X-Band focusing on patch antenna redesign, new manufacturing challenges and initial results of experimental tests.
Multiparameter Analysis, including EMT Markers, on Negatively Enriched Blood Samples from Patients with Squamous Cell Carcinoma of the Head and Neck (2012)

Epithelial to mesenchymal transition (EMT) has been hypothesized as a mechanism by which cells change phenotype during carcinogenesis, as well as tumor metastasis. Whether EMT is involved in cancer metastasis has a specific, practical impact on the field of circulating tumor cells (CTCs). Since the generally accepted definition of a CTC includes the expression of epithelial surface markers, such as EpCAM, if a cancer cell loses its epithelial surface markers (which is suggested in EMT), it will not be separated and/or identified as a CTC. We have developed, and previously reported on the use of, a purely negative enrichment technology enriching for CTCs in the blood of squamous cell carcinoma of the head and neck (SCCHN). This methodology does not depend on the expression of surface epithelial markers. Using this technology, our initial data on SCCHN patient blood indicates that the presence of CTCs correlates with worse disease-free survival. Since our enrichment is not dependent on epithelial markers, we have initiated investigation of the presence of mesenchymal markers in these CTC cells to include analysis of: vimentin, epidermal growth factor receptor, N-cadherin, and CD44. With the aid of confocal microscopy, we have demonstrated not only presumed CTCs that express and/or contain: a nucleus, cytokeratins, vimentin, and either EGFR, CD44, or N-cadherin, but also cells that contain all of the aforementioned proteins except cytokeratins, suggesting that the cells have undergone the EMT process. We suggest that our negative depletion enrichment methodology provides a more objective approach in identifying and evaluating CTCs, as opposed to positive selection approaches, as it is not subjective to a selection bias and can be tailored to accommodate a variety of cytoplasmic and surface markers which can be evaluated to identify a multitude of phenotypic patterns within CTCs from individual patients, including so-called EMT as presented here.
Low-Temperature Aluminum Reduction of Graphene Oxide, Electrical Properties, Surface Wettability, and Energy Storage Applications (2012)

Low-temperature aluminum (Al) reduction is first introduced to reduce graphene oxide (GO) at 100-200 °C in a two-zone furnace. The melted Al metal exhibits an excellent deoxygen ability to produce well-crystallized reduced graphene oxide (RGO) papers with a low O/C ratio of 0.058 (Al-RGO), compared with 0.201 in the thermally reduced one (T-RGO). The Al-RGO papers possess outstanding mechanical flexibility and extremely high electrical conductivities (sheet resistance R(s) ~ 1.75 Ω/sq), compared with 20.12 Ω/sq of T-RGO. More interestingly, very nice hydrophobic nature (90.5°) was observed, significantly superior to the reported chemically or thermally reduced papers. These enhanced properties are attributed to the low oxygen content in the RGO papers. During the aluminum reduction, highly active H atoms from H(2)O reacted with melted Al promise an efficient oxygen removal. This method was also applicable to reduce graphene oxide foams, which were used in the GO/SA (stearic acid) composite as a highly thermally conductive reservoir to hold the phase change material for thermal energy storage. The Al-reduced RGO/SnS(2) composites were further used in an anode material of lithium ion batteries possessing a higher specific capacity. Overall, low-temperature Al reduction is an effective method to prepare highly conductive RGO papers and related composites for flexible energy conversion and storage device applications.
Phosphatidylserine‐related adhesion of human erythrocytes to vascular endothelium (1999)

In pathological conditions such as sickle cell disease, falciparum malaria and diabetes, an abnormal adherence of erythrocytes to endothelium is concomitant with loss of phospholipid asymmetry resulting in phosphatidylserine (PS) exposure. We have investigated the involvement of PS in this interaction by studying adhesion of human erythrocytes, treated with Ca2+-ionophore A23187 in combination with N-ethylmaleimide, to human umbilical vein endothelial cells in a flow-based assay. Results showed that erythrocytes which exposed PS, massively adhered to HUVEC in a Ca2+-dependent manner. This adhesion was inhibited by PS liposomes and by annexin V, giving clear evidence of the PS dependence of these interactions.
The biochemistry and regulation of senescence in chloroplasts (1984)

The biochemical events in chloroplast breakdown are described. Special attention is given to the regulation of RNA, lipid, chlorophyll, and protein degradation. A new hypothesis is presented concerning the relationship between proteolysis and the initial steps in chlorophyll breakdown. It is argued that the dismantling of the chloroplast is a highly organized process and that the concept of loss of chloroplasts by digestion in a lytic vacuole is not tenable.
Modeling and Simulation of Acid Diffusion in Chemically Amplified Resists with Polymer-Bound Acid Generator (2012)

Chemically amplified resists with an acid generator, the anion of which is bound to the polymer through a covalent bond (anion-bound resists), are promising materials for the 16 nm node and beyond. However, their reaction mechanism is unknown. In this study, we propose a proton diffusion model for anion-bound resists. To examine the proton diffusion model, we carried out resist-patterning experiments and a simulation. The calculated latent images corresponded well to the measured line width and estimated chemical gradient. This result suggests that protons diffuse under the electric field produced by anions and induce acid-catalytic reactions in anion-bound resists.
Map Projections: A Reference Manual (1995)

1.Introduction 2. General Theory of Map Projections 3. Map Projections with Straight Parallels 4. Map Projections with Parallels in the Shape of Concentric Circles 5. Map Projections with Parallels in the Shape on Nonconcentric Circles 6. Projections for Topographic and Named Quadrangle Maps 7. Research in Map Projections Best and Ideal Map Projections 8. Numerical Methods in Mathematical Cartography 9. Choice and Identification in Map Projections 10. Problems and Automation in Map Projections 11. Summary of Miscellaneous Map Projections. Normal Normal Default Paragraph Font Default Paragraph Font wdooley$P:\sourcecd.vf7\tnf_books\TF1904.docy?? Unknowny! Times New Roman Times New Roman Symbol Symbol '
Arginine Deprivation and Immune Suppression in a Mouse Model of Alzheimer's Disease (2015)

The pathogenesis of Alzheimer's disease (AD) is a critical unsolved question; and although recent studies have demonstrated a strong association between altered brain immune responses and disease progression, the mechanistic cause of neuronal dysfunction and death is unknown. We have previously described the unique CVN-AD mouse model of AD, in which immune-mediated nitric oxide is lowered to mimic human levels, resulting in a mouse model that demonstrates the cardinal features of AD, including amyloid deposition, hyperphosphorylated and aggregated tau, behavioral changes, and age-dependent hippocampal neuronal loss. Using this mouse model, we studied longitudinal changes in brain immunity in relation to neuronal loss and, contrary to the predominant view that AD pathology is driven by proinflammatory factors, we find that the pathology in CVN-AD mice is driven by local immune suppression. Areas of hippocampal neuronal death are associated with the presence of immunosuppressive CD11c(+) microglia and extracellular arginase, resulting in arginine catabolism and reduced levels of total brain arginine. Pharmacologic disruption of the arginine utilization pathway by an inhibitor of arginase and ornithine decarboxylase protected the mice from AD-like pathology and significantly decreased CD11c expression. Our findings strongly implicate local immune-mediated amino acid catabolism as a novel and potentially critical mechanism mediating the age-dependent and regional loss of neurons in humans with AD.
Learning gestures for interacting with low-fidelity prototypes (2012)

This paper presents an approach to help designers create their own application-specific gestures and evaluate them in user-studies based on low fidelity prototypes of the application they are designing. In order to learn custom gestures, we developed a machine learning tool that uses an anti-unification algorithm to learn based on samples of the gesture provided by the designer.
Induced thermotolerance to apoptosis in a human T lymphocyte cell line (1992)

A brief exposure to elevated temperatures elicits, in all organisms, a transient state of increased heat resistance known as thermotolerance. The mechanism for this thermotolerant state is unknown primarily because it is not clear how mild hyperthermia leads to cell death. The realization that cell death can occur through an active process of self destruction, known as apoptosis, led us to consider whether thermotolerance provides protection against this mode of cell death. Apoptosis is a common and essential form of cell death that occurs under both physiological and pathological conditions. This mode of cell death requires the active participation of the dying cell and in this way differs mechanistically from the alternative mode of cell death, necrosis. Here we show that mild hyperthermia induces apoptosis in a human leukemic T cell line. This is evidenced by chromatin condensation, nuclear fragmentation and the cleavage of DNA into oligonucleosome size units. DNA fragmentation is a biochemical hallmark of apoptosis and requires the activation of an endogenous endonuclease. The extent of DNA fragmentation was proportional to the severity of heat stress for cells heated at 43 degrees C from 30 to 90 minutes. A brief conditioning heat treatment induced a resistance to apoptosis. This was evident as a resistance to DNA fragmentation and a reduction in the number of apoptotic cells after a heat challenge. Resistance to DNA fragmentation developed during a recovery period at 37 degrees C and was correlated with enhanced heat shock protein (hsp) synthesis. This heat-induced resistance to apoptosis suggests that thermotolerant cells have gained the capacity to prevent the onset of this pathway of self-destruction. An examination of this process in heated cells should provide new insights into the molecular basis of cellular thermotolerance.
Flexible sliding frame for gait enhancing mechatronic system (GEMS) (2016)

This paper presents a novel flexible sliding thigh frame for a gait enhancing mechatronic system. With its two-layered unique structure, the frame is flexible in certain locations and directions, and stiff at certain other locations, so that it can fît well to the wearer's thigh and transmit the assisting torque without joint loading. The paper describes the basic mechanics of this 3D flexible frame and its stiffness characteristics. We implemented the 3D flexible frame on a gait enhancing mechatronic system and conducted experiments. The performance of the proposed mechanism is verified by simulation and experiments.
Development of an integrated solid-state generator for light inactivation of food-related pathogenic bacteria (2003)

This paper is concerned with the design and performance of a fully integrated solid-state Marx generator, which has been developed to drive a UV flashlamp for use in microbiological inactivation. The generator has an output voltage rating of 3 kV and a peak current rating of 2 kA, although the modular approach taken allows for a number of voltage and current ratings to be achieved. The generator is constructed using a number of series- and parallel-connected 1.2 kV insulated-gate-bipolar-transistor (IGBT) switched capacitors. Switching of the IGBT modules is controlled by an optical signal. Details are given of how the optimum IGBT gate-drive circuit is achieved using optical components and a pass-through wire to provide the required energies for individual IGBT modules. The generator is demonstrated as the driver of a UV flashlamp used for inactivation of food-related pathogenic bacteria such as E. coli and Salmonella. The performance of the Marx generator over a period of 106 pulses is examined, along with the changes that occur in the spectrum of the UV flashlamp during the same period.
Multilocus Sequence Typing of Total-Genome-Sequenced Bacteria (2012)

Accurate strain identification is essential for anyone working with bacteria. For many species, multilocus sequence typing (MLST) is considered the "gold standard" of typing, but it is traditionally performed in an expensive and time-consuming manner. As the costs of whole-genome sequencing (WGS) continue to decline, it becomes increasingly available to scientists and routine diagnostic laboratories. Currently, the cost is below that of traditional MLST. The new challenges will be how to extract the relevant information from the large amount of data so as to allow for comparison over time and between laboratories. Ideally, this information should also allow for comparison to historical data. We developed a Web-based method for MLST of 66 bacterial species based on WGS data. As input, the method uses short sequence reads from four sequencing platforms or preassembled genomes. Updates from the MLST databases are downloaded monthly, and the best-matching MLST alleles of the specified MLST scheme are found using a BLAST-based ranking method. The sequence type is then determined by the combination of alleles identified. The method was tested on preassembled genomes from 336 isolates covering 56 MLST schemes, on short sequence reads from 387 isolates covering 10 schemes, and on a small test set of short sequence reads from 29 isolates for which the sequence type had been determined by traditional methods. The method presented here enables investigators to determine the sequence types of their isolates on the basis of WGS data. This method is publicly available at www.cbs.dtu.dk/services/MLST.
Optimal tuning of bacterial sensing potential (2009)

Through production and sensing of small signal molecules, quorum sensing (QS) enables bacteria to detect changes in their density and regulate their functions accordingly. QS systems are tremendously diverse in terms of their specific sensory components, the biochemical and transport properties of signaling molecules, their target functions and the context in which QS-mediated functions are activated. Cutting across this diversity, however, the central architecture of QS systems is universal; it comprises signal synthesis, secretion, degradation and detection. We are thus able to derive a general metric for QS 'sensing potential' based on this 'core' module. The sensing potential quantifies the ability of a single bacterium to sense the dimensions of its microenvironment. This simple metric captures the dominant activation properties of diverse QS systems, giving a concise description of the sensing characteristics. As such, it provides a convenient quantitative framework to study the phenotypic effects of QS characteristics. As an example, we show how QS characteristics uniquely determine the scenarios in which regulation of a typical QS-controlled function, such as exoenzyme secretion, becomes advantageous.
Electro-thermal simulation: a new subsystem in Mentor Graphics IC Design flow (2009)

New electro-thermal simulation subsystem was introduced into Mentor Graphics IC Design flow. The subsystem incorporates IC thermal simulation tool “Overheat”, dispatcher “ETh SimCoupler” as the simulation manager and layout converter “ETh Model Generator”. Application example of power voltage regulator IC simulation is described. A good agreement between simulated and IR-camera measured temperature pictures is achieved.
The Effects of Low Level Laser Irradiation on Gingival Inflammation (2009)

A general conclusion can be drawn that low level laser irradiation (semiconductor, 670 nm) can be used as a successful physical adjuvant method of treatment, which, together with traditional periodontal therapy, leads to better and longer-lasting therapeutic results.
Het koloniale verleden van binnenuit: het begin en einde van Nederlands-Indië. (2021)

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