gebhart/patrepeval huggingface dataset:
Abstract 3187: Engineering a new generation of cell therapies for solid tumor oncology using the SQZ platform (2019)

Effective T cell priming is crucial to induce anti-tumor CD8+ T cell responses and requires the efficient presentation of antigen on major histocompatibility complex class I (MHC-I) by antigen presenting cells (APCs). Previous efforts using dendritic cells to prime CD8+ T cell responses have proven difficult due to limited cell availability in the blood and challenges delivering antigen to the APC cytosol, a necessary step for MHC-I presentation and CD8+ T cell activation. To overcome this limitation, we deliver antigen directly to the cytosol of target APCs using the microfluidics-based SQZ platform. SQZ uniquely facilitates antigen loading into both professional and unconventional APCs, including B cells, T cells, and heterogenous populations of cells, which can be easily obtained directly from the blood. Protein and peptide antigens are delivered using SQZ to each of these APCs effectively, leading to efficient presentation of immunogenic epitopes on MHC-I. Here, we demonstrate that murine SQZ-APCs can stimulate antigen-specific CD8+ T cell responses in vitro and in vivo as measured by expansion of antigen-specific T cells and production of IFNγ. In the TC-1 tumor model for HPV-associated cancers, antigen-loaded SQZ-APCs have strong anti-tumor effects both prophylactically and therapeutically. Following therapeutic immunization, the anti-tumor responses correlate with an increase in antigen-specific CD8+ tumor infiltrating lymphocytes compared to untreated mice. In addition, compared to a traditional subcutaneous peptide vaccine, SQZ-APCs elicit a five-fold greater intratumoral CD8+ T cell response and drive significantly more tumor growth inhibition. Importantly, this SQZ-enabled cancer cell therapy translates to human B cells, T cells, and heterogenous populations of cells engineered to function as APCs. When a peptide is delivered to the cytosol using SQZ, all of these primary human cells activate antigen-specific CD8+ T cell responses in vitro by stimulation of IFNγ from antigen-specific CD8+ T cell responders. In comparison to cells incubated in the presence of peptide antigen, SQZ-APCs stimulate a 10-fold increase in IFNγ production from antigen-specific CD8+ responder T cells (n=13 donors). Finally, the SQZ process has been scaled to engineer human SQZ-APCs in preparation for clinical trials with a throughput of greater than 4 billion cells SQZ’d per minute. Collectively, these findings highlight the significant clinical potential of the SQZ platform to engineer potent APCs for a new generation of cancer cell therapies.Citation Format: Kelan A. Hlavaty, Matthew G. Booty, Scott Loughhead, Katarina Blagovic, Alfonso Vicente-Suarez, Defne Yarar, Howard Bernstein, Armon Sharei. Engineering a new generation of cell therapies for solid tumor oncology using the SQZ platform [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2019; 2019 Mar 29-Apr 3; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2019;79(13 Suppl):Abstract nr 3187.
Effect of irradiation on collagen solutions in relation to biomedical applications. (1989)

The aim of this work was to investigate physico-chemical properties of collagen solutions and evaluate the influence of ionizing radiation on: formation of collagen gels, thermal stability of protein, amino acid composition and cross-linking of collagen. Collagen soluble in acetic acid was prepared from bovine Achilles tendons by the enzymatic (pepsin) method. The results obtained in this study are discussed in view of the possible use of radiation cross-linked collagen for the biomaterials.
Encapsulants used in flip-chip packages (1993)

A systematic approach to quantifying the viscosity and flow coverage, thermal expansion, modulus, glass transition temperature, filler size, alpha activity, adhesion, ionics content, fracture toughness, and electrical properties of low-thermal-expansion encapsulants is presented. The various test methods and their relevant technical aspects are discussed. Results achieved on a few selected materials are presented as examples.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Intact p53-Dependent Responses in miR-34–Deficient Mice (2012)

MicroRNAs belonging to the miR-34 family have been proposed as critical modulators of the p53 pathway and potential tumor suppressors in human cancers. To formally test these hypotheses, we have generated mice carrying targeted deletion of all three members of this microRNA family. We show that complete inactivation of miR-34 function is compatible with normal development in mice. Surprisingly, p53 function appears to be intact in miR-34-deficient cells and tissues. Although loss of miR-34 expression leads to a slight increase in cellular proliferation in vitro, it does not impair p53-induced cell cycle arrest or apoptosis. Furthermore, in contrast to p53-deficient mice, miR-34-deficient animals do not display increased susceptibility to spontaneous, irradiation-induced, or c-Myc-initiated tumorigenesis. We also show that expression of members of the miR-34 family is particularly high in the testes, lungs, and brains of mice and that it is largely p53-independent in these tissues. These findings indicate that miR-34 plays a redundant function in the p53 pathway and suggest additional p53-independent functions for this family of miRNAs.
In vivo and in vitro repeatability of Hartmann-Shack aberrometry (2003)

Repeatability of Hartmann-Shack aberrometry by the Zywave wavefront sensor was not satisfactory, particularly for small amounts of HOAs. Under these conditions, aberrometry measurements should be repeated several times and outliers should be excluded in calculating the means.
Disseminated Tumor Cells in Prostate Cancer Patients after Radical Prostatectomy and without Evidence of Disease Predicts Biochemical Recurrence (2009)

Approximately 70% of men undergoing radical prostatectomy had DTC detected in their bone marrow prior to surgery, suggesting that these cells escape early in the disease. Although preoperative DTC status does not correlate with pathologic risk factors, persistence of DTC after radical prostatectomy in NED patients was an independent predictor of recurrence.
Elimination of spiral chaos by pulse entrainment in the Aliev-Panfilov model (2005)

Pulse entrainment between two excitatory media is studied in the Aliev-Panfilov model. We show that a spiral chaos in the continuous excitatory medium can be eliminated by a grid network using the pulse entrainment. This mechanism may be applied to the cardiac system, where the ventricular fibrillation is interpreted as the spiral chaos and the Purkinje fibers act as the grid network.
Olive (Olea europaea L.) Leaf Polyphenols Improve Insulin Sensitivity in Middle-Aged Overweight Men: A Randomized, Placebo-Controlled, Crossover Trial (2013)

Supplementation with olive leaf polyphenols for 12 weeks significantly improved insulin sensitivity and pancreatic β-cell secretory capacity in overweight middle-aged men at risk of developing the metabolic syndrome.
Inducing Stable α + β Microstructures during Selective Laser Melting of Ti-6Al-4V Using Intensified Intrinsic Heat Treatments (2017)

Selective laser melting is a promising powder-bed-based additive manufacturing technique for titanium alloys: near net-shaped metallic components can be produced with high resource-efficiency and cost savings [...].
A Power-Scalable DCO for Multi-Standard GSM/WCDMA Frequency Synthesizers (2014)

A Digitally Controlled Oscillator (DCO) whose power consumption can be reconfigured while maintaining an almost constant phase-noise figure-of-merit (FoM). This is achieved by using either a single-switch-pair or a complementary (i.e., double-switch-pair) oscillator topology, without disturbing the optimized LC tank of the DCO. The optimal power consumption in the complementary (P-N) configuration is reduced by 75% compared to the single-switch-pair (N-only) configuration, while the FoM is kept constant. Measurements on a 55 nm CMOS 4 GHz DCO prototype show a minimum phase noise of -129.3 dBc/Hz at 2 MHz offset from the carrier in the P-N configuration, and of -134.7 dBc/Hz in the N-only configuration, with a phase noise difference very close to the 6 dB expected from theory. The current consumption is 6 mA and 24 mA, respectively, resulting in approximately the same FoM of -185 dBc/Hz.
Design and Implementation of Network Chip for Wireless and Wired Peripherals with Serial Communication (2016)

Wireless sensor networks (WSNs) have gained worldwide attention in recent years. Unlike traditional networks, a WSN has its own design and resource constraints. Resource constraints include a limited amount of energy, short communication range, low bandwidth, and limited processing and storage in each node. However, the current connect number, sampling rate, and signal types of sensors are generally restricted by the device. In this paper, to solve these problems, a new method is proposed to design and implementation of network chip for wireless and wired peripherals with serial communication, in which filed programmable gate array (FPGA) is adopted as the core controller. Thus, it can read data in in real time with high speed on different sensor data. The standard of IEEE1451.2 intelligent sensor interface specification is adopted in this design. A new solution is provided for the traditional sensor data acquisitions. The device is combined with the FPGA programmable technology and the standard of IEEE1451.2 intelligent sensor specification. The performance of the proposed system is verified and good effects are achieved in practical application.
Adrenal Insufficiency, Heart Rate Variability, and Complex Biologic Systems: A Study of 1,871 Critically Ill Trauma Patients (2007)

Reduced heart-rate variability, a potential measurement of complex biologic systems, is associated with cosyntropin-confirmed AI; improved in patients responding to steroid therapy; and is a noninvasive, real-time biomarker suggesting AI.
Hedging (2008)

Hedging is defined with a state-space model of risky outcomes. Full and partial hedging are compared, and the feasible set of hedging positions related to the available collection of traded assets. Three types of counter-parties for hedging trades are distinguished. The risk premium for a hedging asset is defined, and its relationship to economy-wide risk factors explained. The case of mean-variance preferences provides a useful formula for the optimal hedge position. Corporations undertake many hedging transactions, even though the shareholders of the corporation do not typically benefit from any risk reduction. Some explanations of corporate hedging are set out.
Absence of right ventricular isovolumic relaxation in open-chest anesthetized dogs (1992)

During the left ventricular (LV) pump cycle, peak negative first derivative of pressure vs. time (dP/dt) occurs very close to the end of LV ejection, and there is a well-defined isovolumic relaxation period. Despite similarities between the right ventricular (RV) and LV pump cycles, recent studies indicate uncertainty as to whether peak negative RV dP/dt occurs simultaneously with RV end ejection and whether there is an isovolumic relaxation period during the RV pump cycle. To study these questions, we recorded relative timing of peak negative RV dP/dt, RV end ejection, and right atrial-RV pressure crossover in the open-chest anesthetized dog. The data demonstrate that peak negative RV dP/dt occurs an average of 60 ms before end ejection and that there is no RV isovolumic relaxation period. These findings have implications for the possible use of peak negative RV dP/dt as a marker of RV end ejection and for how time constants of pressure decay obtained during RV relaxation can be interpreted.
Vibration-displacement measurements based on the phase tracking of an optical fiber Michelson interferometer (2009)

A vibration-displacement measurement system based on tracking the phase variation of an optical fiber Michelson interferometer with electronic feedback loops is presented. The measurement system includes two sets of electronic feedback loops. One electronic feedback loop is used to compensate for the low frequency drifts in the phase of the interferometric signal that results from environmental disturbances, while the other one is used to track the phase variation induced by the measured vibration displacement, and thus realize the measurement of the vibration displacement and provide a sense of direction of the displacement simultaneously. The measurement system is designed to be capable of measuring vibration displacement with frequencies ranging from 1.5 to 200 Hz, with measurement resolution reaching 13 nm.
Cryopreservation of human oocytes: a review of current problems and perspectives (1996)

It is well recognized that the ability to cryopreserve unfertilized human oocytes would make a significant contribution to infertility treatment. However, despite considerable interest, very few successful pregnancies have arisen from cryopreserved oocytes after thawing, insemination and transfer of the subsequent embryo. The reasons for this lack of progress may well result from a dearth of information on how the various biophysical changes during a cryopreservation regimen affect human oocyte function. Recently, fundamental studies on the effects of cooling, membrane permeability, cryoprotectant addition and ice formation have been performed on human oocytes by a number of groups, and these form the basis of the current review. It is likely that successful human oocyte cryopreservation will only follow once these factors are fully understood, but the existing base of knowledge should provide a platform for further improvements in the techniques currently employed.
Divergent roles of immune cells and their mediators in pain (2017)

Chronic pain is a major debilitating condition that is difficult to treat. Although chronic pain may appear to be a disorder of the nervous system, crucial roles for immune cells and their mediators have been identified as important contributors in various types of pain. This review focuses on how the immune system regulates pain and discusses the emerging roles of immune cells in the initiation or maintenance of chronic pain. We highlight which immune cells infiltrate damaged nerves, the dorsal root ganglia, spinal cord and tissues around free nerve endings and discuss through which mechanisms they control pain. Finally we discuss emerging roles of the immune system in resolving pain and how the immune system contributes to the transition from acute to chronic pain. We propose that targeting some of these immune processes may provide novel therapeutic opportunities for the treatment of chronic pain.
Anti-podoplanin Monoclonal Antibody LpMab-7 Detects Metastatic Lesions of Osteosarcoma (2015)

Osteosarcoma is the most common primary malignant bone tumor and is highly metastatic to the lungs. Therefore, the development of a novel molecular targeting therapy against metastasis of osteosarcoma is necessary. A platelet aggregation-inducing factor, podoplanin/aggrus, is involved in tumor metastasis. Furthermore, podoplanin expression was reported to be involved in the poor prognosis of osteosarcoma patients. However, the association between podoplanin expression and metastasis of osteosarcoma remains unclear because of the lack of highly sensitive anti-podoplanin monoclonal antibodies (MAbs). In this study, we used a novel anti-podoplanin MAb, LpMab-7, which is more sensitive than well-known anti-podoplanin MAbs in immunohistochemistry. Immunohistochemical analysis using LpMab-7 showed that podoplanin expression at primary lesions is observed in 15 out of 16 (93.8%) cases. Furthermore, podoplanin expression at metastatic lesions was higher compared with primary lesions in three out of four (75%) cases with lung metastasis. Because LpMab-7 has high sensitivity against podoplanin, it is expected to be useful for molecular targeting therapy for osteosarcomas.
<title>MIT multipoint alignment testbed: technology development for optical interferometry</title> (1991)

A class of proposed space-based astronomical missions requiring large baselines and precision alignment can benefit from the application of Controlled Structures Technology. One candidate mission, that of a 35 meter baseline orbiting optical interferometer, is studied as a focus mission for a testbed for controlled structures research. Interferometry science requirements are investigated and used to design a laboratory testbed which captures the essential architecture, physics and performance requirements of a full scale instrument. Testbed hardware used for identification and control is presented, including an on-board six-axis laser metrology system using state of the art cat's eye retroreflectors. The testbed and research program are discussed in terms of controlled structures design and in terms of the expected benefits to the optical engineering and science communities.
Destruction and reconstruction: Hypoxia and the developing brain (2007)

Preterm infants have a high rate of neurodevelopmental handicap. Recent imaging studies have revealed that adverse outcomes are strongly associated with reduced brain growth and neural complexity in later life. Increasing data suggest that these chronic deficits primarily reflect acute neuronal and glial injury sustained during adverse in utero events, such as exposure to severe hypoxia-ischemia and inflammation. In the present review we examine recent evidence that this chronic impairment is partly due to upregulation of physiological apoptosis, related to input deprivation, and output isolation secondary to acute white and gray matter damage and axonal injury. However, progenitor cells in the subventricular zone (SVZ) are also vulnerable to injury, and loss of part of this critical population likely further compromises brain development. Based on these concepts the impact of proposed interventions such as induced hypothermia and endogenous growth factors are likely to be complex, but potentially offer focused ways of improving the outcomes of premature birth.
On the Geometry of 3D Orientation Measurement Using a New Class of Wireless Angular Position Sensors (2003)

This paper deals with the geometric issues that arise in designing a system for measuring the orientation of an object in three dimensional space using a new class of wireless angular position sensors. The wireless sensors are waveguides that receive and record the electromagnetic energy emitted by a polarized RF source. The angular position of the waveguide relative to the source is indicated by the energy level. A system equipped with multiple waveguides is used as a 3D orientation sensor. This paper explores the geometry for orientation measurement using the system and provides the guidelines for sensor design.
SEGMENTATION OF OVARIAN ULTRASOUND IMAGES USING CELLULAR NEURAL NETWORKS (2004)

Segmentation of ovarian ultrasound images using cellular neural networks (CNNs) is studied in this paper. The segmentation method consists of five successive steps where the first four uses CNNs. In the first step, only rough position of follicles is determined. In the second step, the results are improved by expansion of detected follicles. In the third step, previously undetected inexpressive follicles are determined, while the fourth step detects the position of ovary. All results are joined in the fifth step. The templates for CNNs were obtained by applying genetic algorithm. The segmentation method has been tested on 50 ovarian ultrasound images. The recognition rate of follicles was around 60% and misidentification rate was around 30%.
Features of realization of commercial banks operations on the market of securities (2008)

The specific of realization of operations of Ukraine's commercial banks at the market of equities is examined in the article.
Gandalf: An Intelligent, {End-To-End} Analytics Service for Safe Deployment in {Large-Scale} Cloud Infrastructure (2020)

Modern cloud systems have a vast number of components that continuously undergo changes. Deploying these frequent updates quickly without breaking the system is challenging. In this paper, we present Gandalf, an end-to-end analytics service for safe deployment in a large-scale system infrastructure. Gandalf enables rapid and robust impact assessment of software rollouts to catch bad rollouts before they cause widespread outages. Gandalf monitors and analyzes various fault signals and correlates each signal against all the ongoing rollouts using a spatial and temporal correlation algorithm. Its core decision logic includes an ensemble ranking algorithm that determines which rollout caused the fault signals and a binary classifier that assesses the impact of the fault signals. The analysis result determines whether a rollout is safe to proceed or should be stopped. By using a lambda architecture, Gandalf provides both real-time and long-term deployment monitoring with automated decisions and notifications. Gandalf has been running in production in Microsoft Azure for more than 18 months, serving both data-plane and control-plane components. It achieves 92.4% precision and 100% recall (no high-impact service outages in Azure Compute were caused by bad rollouts) for data-plane rollouts. For control-plane rollouts, Gandalf achieves 94.87% precision and 99.84% recall.
Myeloma cells act as tolerogenic antigen‐presenting cells and induce regulatory <scp>T</scp> cells <i>in vitro</i> (2014)

Regulatory T cells (Tregs) are essential for maintenance of self-tolerance; however, tumor cells can exploit the tolerance to escape the immune system. We investigated the Tregs frequency in patients with multiple myeloma (MM) and in those with monoclonal gammopathy of undetermined significance (MGUS), and found that CD4(+) FoxP3(+) and CD8(+) FoxP3(+) Tregs were significantly increased in patients with MM and correlated with the active phase. Both Tregs subsets were expanded in cocultures of CD3(+) lymphocytes and fresh CD138(+) MM plasma cells or RPMI8226 and U266 cell lines and functioned as natural (n) and inducible (i) Tregs insofar as they inhibited the proliferation of stimulated CD3 lymphocytes via contact-dependent and contact-independent pathways. Induction of Tregs by MM plasma cells required a contact-dependent pathway, implying antigen recognition by T cells. MM plasma cells acted as immature and tolerogenic antigen-presenting cells (APCs), in that they displayed low CD80/CD86 expression associated with a phagocytic activity. By acting as immature APCs, MM plasma cells plausibly expand (n)Tregs and (i)Tregs both through conversion of CD3(+) FoxP3(-) into CD3(+) FoxP3(+) T cells and proliferation of CD3(+) FoxP3(+) T cells, which may suppress the anti-MM immune response.
Improving Disk Array Reliability Through Expedited Scrubbing (2010)

Disk scrubbing periodically scans the contents of a disk array to detect the presence of irrecoverable read errors and reconstitute the contents of the lost blocks using the built-in redundancy of the disk array. We address the issue of scheduling scrubbing runs in disk arrays that can tolerate two disk failures without incurring a data loss, and propose to start an urgent scrubbing run of the whole array whenever a disk failure is detected. Used alone or in combination with periodic scrubbing runs, these expedited runs can improve the mean time to data loss of disk arrays over a wide range of disk repair times. As a result, our technique eliminates the need for frequent scrubbing runs and the need to maintain spare disks and personnel on site to replace failed disks within a twenty-four hour interval.