gebhart/patrepeval huggingface dataset:
HG-09 * INFANTILE GLIOBLASTOMA WITH SARCOMATOUS HISTOLOGY DRIVEN BY ETV6:NTRK3 FUSIONS ARE SENSITIVE TO TRK INHIBITION BY PHA-848125 (2015)

Approximately 1% of all paediatric brain tumours occur at less than one year of age, and glioblastoma (GBM) account for only 2-9% of these. Their clinical presentation, location, histology, behaviour, response to therapy and outcome differ from GBMs of older children and adults, and they do not appear to be associated with known histone H3 or IDH1-driven subgroups. Recent genome and RNA sequencing data highlighted recurrent NTRK1-3 fusions in 4/10 non-brainstem GBM under the age of 3 years, including one case of ETV6-NTRK3 in a cerebral hemispheric tumour diagnosed at 1 month old. These fusions are usually associated with congenital mesoblastic nephroma and congenital infantile fibrosarcoma, and upon review of a series of cases diagnosed as glioblastoma under the age of 1yr, 6/14 were found to present with a predominant spindle cell morphology, a relatively circumscribed interface with adjacent brain, and without prominent infiltration. These tumours were positive for GFAP as well as desmin, CD56 and CD99. One case was diagnosed in utero by ultrasound, and at birth underwent surgical resection from which primary cell cultures were established under stem cell conditions on laminin-coated plates. Exome and RNA sequencing revealed the presence of the ETV6:NTRK3 fusion transcript as the sole somatic alteration, validated by RT-PCR, Sanger sequencing and FISH. 450K methylation profiling clustered the tumour with desmoplastic infantile ganglioglioma rather than subgroups of paediatric GBM. Primary cells treated with the CNS-penetrant small molecule Trk inhibitor PHA-848125, resulted in marked sensitivity (GI50 = 200nM) compared to cerebral hemispheric (H3 wild-type) and thalamic (H3.3K27M) paediatric GBM cells (GI50 = 1.8-4.6mM). A subset of infantile/congenital brain tumours classified as GBM appear to represent a distinct entity more closely related, biologically and histologically, to mesenchymal cancers. Agents targeting ETV:NTRK3 may be a useful novel treatment strategy in these children.
Targeting AKT1-E17K and the PI3K/AKT Pathway with an Allosteric AKT Inhibitor, ARQ 092 (2015)

As a critical component in the PI3K/AKT/mTOR pathway, AKT has become an attractive target for therapeutic intervention. ARQ 092 and a next generation AKT inhibitor, ARQ 751 are selective, allosteric, pan-AKT and AKT1-E17K mutant inhibitors that potently inhibit phosphorylation of AKT. Biochemical and cellular analysis showed that ARQ 092 and ARQ 751 inhibited AKT activation not only by dephosphorylating the membrane-associated active form, but also by preventing the inactive form from localizing into plasma membrane. In endometrial PDX models harboring mutant AKT1-E17K and other tumor models with an activated AKT pathway, both compounds exhibited strong anti-tumor activity. Combination studies conducted in in vivo breast tumor models demonstrated that ARQ 092 enhanced tumor inhibition of a common chemotherapeutic agent (paclitaxel). In a large panel of diverse cancer cell lines, ARQ 092 and ARQ 751 inhibited proliferation across multiple tumor types but were most potent in leukemia, breast, endometrial, and colorectal cancer cell lines. Moreover, inhibition by ARQ 092 and ARQ 751 was more prevalent in cancer cell lines containing PIK3CA/PIK3R1 mutations compared to those with wt-PIK3CA/PIK3R1 or PTEN mutations. For both ARQ 092 and ARQ 751, PIK3CA/PIK3R1 and AKT1-E17K mutations can potentially be used as predictive biomarkers for patient selection in clinical studies.
Genomic High-Resolution Profiling of Single CKpos/CD45neg Flow-Sorting Purified Circulating Tumor Cells from Patients with Metastatic Breast Cancer (2014)

This work flow enabled effective CTC isolation and provided insights into genomic alterations of CTCs in metastatic breast cancer. This approach might facilitate further molecular characterization of rare CTCs to increase understanding of their biology and as a basis for their molecular screening in the clinical setting.
Modeling the Impact of Manufacturing Uncertainties on Lithium-Ion Batteries (2020)

This paper describes and analyzes the propagation of uncertainties from the lithium-ion battery electrode manufacturing process to the structural electrode parameters and the resulting varying electrochemical performance. It uses a multi-level model approach, consisting of a process chain simulation and a battery cell simulation. The approach enables to analyze the influence of tolerances in the manufacturing process on the process parameters and to study the process-structure-property relationship. The impact of uncertainties and their propagation and effect is illustrated by a case study with four plausible manufacturing scenarios. The results of the case study reveal that uncertainties in the coating process lead to high deviations in the thickness and mass loading from nominal values. In contrast, uncertainties in the calendering process lead to broad distributions of porosity. Deviations of the thickness and mass loading have the highest impact on the performance. The energy density is less sensitive against porosity and tortuosity as the performance is limited by theoretical capacity. The latter is impacted only by mass loading. Furthermore, it is shown that the shape of the distribution of the electrochemical performance due to parameter variation aids to identify, whether the mean manufacturing parameters are close to an overall performance optimum.
Rapid assessment of <i>in vivo</i> cholinergic transmission by amperometric detection of changes in extracellular choline levels (2004)

Conventional microdialysis methods for measuring acetylcholine (ACh) efflux do not provide sufficient temporal resolution to relate cholinergic transmission to individual stimuli or behavioral responses, or sufficient spatial resolution to investigate heterogeneities in such regulation within a brain region. In an effort to overcome these constraints, we investigated a ceramic-based microelectrode array designed to measure amperometrically rapid changes in extracellular choline as a marker for cholinergic transmission in the frontoparietal cortex of anesthetized rats. These microelectrodes exhibited detection limits of 300 nm for choline and selectivity (> 100 : 1) of choline over interferents such as ascorbic acid. Intracortical pressure ejections of choline (20 mm, 66-400 nL) and ACh (10 and 100 mm, 200 nL) dose-dependently increased choline-related signals that were cleared to background levels within 10 s. ACh, but not choline-induced signals, were significantly attenuated by co-ejection of the acetylcholinesterase inhibitor neostigmine (Neo; 100 mm). Pressure ejections of drugs known to increase cortical ACh efflux, potassium (KCl; 70 mm, 66, 200 nL) and scopolamine (Scop; 10 mm, 200 nL), also markedly increased extracellular choline signals, which again were inhibited by Neo. Scop-induced choline signals were also found to be tetrodotoxin-sensitive. Collectively, these findings suggest that drug-induced increases in current measured with these microelectrode arrays reflect the oxidation of choline that is neuronally derived from the release and subsequent hydrolysis of ACh. Choline signals assessed using enzyme-selective microelectrode arrays may represent a rapid, sensitive and spatially discrete measure of cholinergic transmission.
The protective effect of melatonin on renal ischemia–reperfusion injury in the rat (2002)

Oxygen free radicals are considered to be important components involved in the pathophysiological tissue alterations observed during ischemia-reperfusion (I/R). In this study, we investigated the putative protective effects of melatonin treatment on renal I/R injury. Wistar albino rats were unilaterally nephrectomized and subjected to 45 min of renal pedicle occlusion followed by 1, 3, 6, 24, 48 hr or 1 wk of reperfusion. Melatonin (10 mg/kg, s.c.) or vehicle was administered twice, 15 min prior to ischemia and immediately before the reperfusion period. At the end of the reperfusion periods, rats were decapitated. Kidney samples were taken for histological examination or the determination of renal malondialdehyde (MDA) and glutathione (GSH) levels, myeloperoxidase (MPO) activity and protein oxidation (PO). Serum creatinine and blood urea nitrogen (BUN) concentrations were measured for the evaluation of renal function. The results revealed that I/R induced nephrotoxicity, as evidenced by increases in BUN and creatinine levels at each time point, was reversed by melatonin treatment. The decrease in GSH and increases in MDA, MPO and PO induced by I/R indicated that renal injury involves free radical formation. As melatonin administration reversed these oxidant responses, improved renal function and microscopic damage, it seems likely that melatonin protects kidney tissue against oxidative damage.
A Highly Conserved Motif within the NH2-terminal Coiled-coil Domain of Angiopoietin-like Protein 4 Confers Its Inhibitory Effects on Lipoprotein Lipase by Disrupting the Enzyme Dimerization (2009)

Lipoprotein lipase (LPL) is a principal enzyme responsible for the clearance of chylomicrons and very low density lipoproteins from the bloodstream. Two members of the Angptl (angiopoietin-like protein) family, namely Angptl3 and Angptl4, have been shown to inhibit LPL activity in vitro and in vivo. Here, we further investigated the structural basis underlying the LPL inhibition by Angptl3 and Angptl4. By multiple sequence alignment analysis, we have identified a highly conserved 12-amino acid consensus motif that is present within the coiled-coil domain (CCD) of both Angptl3 and Angptl4, but not other members of the Angptl family. Substitution of the three polar amino acid residues (His(46), Gln(50), and Gln(53)) within this motif with alanine abolishes the inhibitory effect of Angptl4 on LPL in vitro and also abrogates the ability of Angptl4 to elevate plasma triglyceride levels in mice. The CCD of Angptl4 interacts with LPL and converts the catalytically active dimers of LPL to its inactive monomers, whereas the mutant protein with the three polar amino acids being replaced by alanine loses such a property. Furthermore, a synthetic peptide consisting of the 12-amino acid consensus motif is sufficient to inhibit LPL activity, although the potency is much lower than the recombinant CCD of Angptl4. In summary, our data suggest that the 12-amino acid consensus motif within the CCD of Angptl4, especially the three polar residues within this motif, is responsible for its interaction with and inhibition of LPL by blocking the enzyme dimerization.
Detecting and tracking people using an RGB-D camera via multiple detector fusion (2011)

The goal of personal robotics is to create machines that help us with the tasks of daily living, co-habiting with us in our homes and offices. These robots must interact with people on a daily basis, navigating with and around people, and approaching people to serve them. To enable this coexistence, personal robots must be able to detect and track people in their environment. Excellent progress has been made in the vision community in detecting people outdoors, in surveillance scenarios, in Internet images, or in specific scenarios such as video game play in living rooms. The indoor robot perception problem differs, however, in that the platform is moving, the subjects are frequently occluded or truncated by the field-of-view, there is large scale variation, the subjects take on a wider range of poses than pedestrians, and computation must take place in near real time. In this paper, we describe a system for detecting and tracking people from image and depth sensors on board a mobile robot. To cope with the challenges of indoor mobile perception, our system combines an ensemble of detectors in a unified framework, is efficient, and has the potential to incorporate multiple sensor inputs. The performance of our algorithm surpasses other approaches on two challenging data sets, including a new robot-based data set.
Synaptic remodeling during aging and in Alzheimer's disease (2006)

Cognitive functioning is dependent on synapse density in the brain. Factors modulating synapse density might include the balance between synaptic pruning and sprouting. Loss of synapses during aging might explain cognitive decline and while previous reports have suggested a 10-15% synapse loss occurs during the normal aging process, more recent studies have found that decline in synaptic density only occurs after 65 years of age. In this context, the main objective of this manuscript is to discuss the findings of our 1993 study in light of more recent studies in aging, synapses and Alzheimer's disease.
Backsheet metallization of IBC silicon solar cells (2013)

A simplified interconnection/metallization method for interdigitated back-contact (IBC) silicon solar cells is reported. A patterned PEDOT:PSS seed layer was inkjet printed on the backsheet. After curing, the seed layer pattern was electroplated first with Cu and then with eutectic Sn-Bi. During lamination at 150 °C, the eutectic Sn-Bi alloy melts and provides good ohmic contact between the IBC solar cells and the electroplated Cu on the backsheet, thus simultaneously achieving metallization and interconnection. The method eliminates the need to form thick metal conductors on the IBC cells. Furthermore, the moderate conductivity of the PEDOT:PSS seed layer lines results in tapered Cu conductors with the finger height reducing as a function of distance from the contacted busbar. This tapered metal grid can reduce the mass of Cu required for a 1% resistive power loss by 24%.
Prosopagnosia as a Type of Conversion Disorder (2018)

We propose that our patient's adverse childhood experiences led to the development of a particular personality and coping style that "primed" him for a later abnormal emotional and behavioural response when confronted with reminders of his traumatic background. Further interdisciplinary studies are required to further elucidate the neurobiological basis for this condition.
Computing persistent homology (2004)

We study the homology of a filtered d-dimensional simplicial complex K as a single algebraic entity and establish a correspondence that provides a simple description over fields. Our analysis enables us to derive a natural algorithm for computing persistent homology over an arbitrary field in any dimension. Our study also implies the lack of a simple classification over non-fields. Instead, we give an algorithm for computing individual persistent homology groups over an arbitrary PIDs in any dimension.
Positioning in Breast MR Imaging to Optimize Image Quality (2014)

Improper positioning of the breasts in a dedicated breast coil causes inhomogeneous fat saturation as well as other artifacts that decrease the sensitivity of breast magnetic resonance imaging. Improper positioning can create artifacts that can obscure a malignancy or cause it to be missed. Goals of proper positioning include imaging the maximum area of breast tissue, minimizing skin folds, and achieving homogeneous fat suppression and nondeformed breast parenchyma. Review of prior images gives the technologist an impression of what the positioning and imaging challenges may be in each patient before the patient enters the imaging unit. Checking the triplane localizer images and repositioning as necessary before any diagnostic or interventional imaging is key. Using a fat saturation pad, changing the arm position, or "rolling" the patient may be considered in difficult cases. Padding to support the patient in an oblique position, using angled sponges to increase breast compression thickness, and raising the grid to access posterior lesions may be helpful in targeting difficult-to-access lesions for biopsy. Using the presented positioning techniques and suggestions, in addition to strict attention to detail before imaging, will improve image quality, decrease imaging time and suboptimal images, and limit the need for repeat imaging studies.
An IoT-oriented Fast Prototyping Platform for BLE-based Star Topology Networks (2019)

The Internet of Things (IoT) is characterized by many technologies, standards, tools and devices for a wide range of application fields and often, for the end-users (makers and developers), is hard to orientate in an equally wide range of offers from various manufacturers. In recent years, the Bluetooth Low Energy (BLE) communication protocol is achieving a large portion of the market, thanks to its low-power and low-cost orientation and its pervasiveness in mobile devices, like smartphones. For these reasons, BLE is increasingly used in IoT-oriented Wireless Personal Area Networks (WPAN), where a small set of devices arranged in star topology network and connected to a smartphone and a Wi-Fi gateway, can cover a large number of monitoring and controlling use case scenarios. This work presents the ST’s STM32 Open Development Environment (ODE), a complete suite of hardware and software tools representing a reference point for end-users willing to create BLE-based star topology networks for a wide range of applications. Through a simple use case in a smart home context, it is shown how all provided tools can be used to fast prototype applications addressing all user requirements.
Sulphate polyanions prolong the incubation period of scrapie-infected hamsters (1992)

The effect of the organic sulphated polyanions, pentosan sulphate (SP54), dextran sulphate 500 (DS500) and suramin, have been tested on golden Syrian hamsters infected with the 263K strain of scrapie by the intraperitoneal (i.p.) or the intracerebral route. SP54 had the greatest effect in prolonging the incubation period of the disease when administered within 2 h of the i.p. inoculum. The same amount of SP54 given 24 h after scrapie inoculation had a potent effect in some animals and no effect in others. This result suggests that SP54 inhibits the uptake of the scrapie agent into the nerve endings and/or carrier cells at the site of the inoculum, i.e. the peritoneum, and that this event occurs in about 24 h. DS500 had a similar although less potent effect (22.4 days delay during the incubation period) than SP54 (54.4 days) when administered within 2 h of scrapie injection by the i.p. route, and suramin had only a minimal effect (10 days). This study suggests that treatment of scrapie and related spongiform encephalopathies of animals and man is possible only before the agent has reached the clinical target areas of the brain.
Retargetable assembly code generation by bootstrapping (1994)

In a hardware/software codesign environment compilers are needed that map software components of a partitioned system behavioral description onto a programmable processor. Since the processor structure is not static, but can repeatedly change during the design process, the compiler should be retargetable in order to avoid manual compiler adaption for each alternative architecture. A restriction of existing retargetable compilers is that they only generate microcode for the target architecture instead of machine-level code. We introduce a bootstrapping technique permitting to translate high-level language (HLL) programs into real machine-level code using a retargetable microcode compiler. Retargetability is preserved, permitting to compare different architectural alternatives in a codesign framework within relatively short time. >
Optical bit-rate conversion and bit-stream time reversal by the use of swept-carrier frequency-selective optical data storage techniques (1995)

We have demonstrated real-time all-optical bit-rate conversion and bit-sequence time reversal, using a variation of the swept-carrier frequency-selective optical storage/retrieval process. Working in the Mbit/s regime, we obtained order-of-magnitude bit-rate enhancements and reductions. Output signals appear in a time-forward or time-reversed format after an adjustable buffering storage time. Analysis of the method of its potential indicates applicability at substantially higher data rates.
A remote controller for home and office appliances by telephone (1998)

This paper describes the design and development of a phone-based remote controller for home and office automation. The circuit is designed based on the Turkish telephone standards and connected to the telephone network just like any normal telephone sets. Any tone dialing dual tone multiple frequency (DTMF) telephone set or hand-held tone dialer may be used to send commands to the control unit, and remotely control, a wide range of mains appliances in homes and offices. The designed circuit can also detect the user identification number for preventing non-authorized use of the control unit. The feedback signal informs the user about the results of the commands.
Single‐Wall Carbon Nanotube–Ferrocene Nanohybrids: Observing Intramolecular Electron Transfer in Functionalized SWNTs (2003)

A long-lived charge-separated state, SWNT.−–Fc.+, is formed by photoinduced electron transfer in a novel single-wall carbon nanotube–ferrocene (SWNT–Fc) nanohybrid. The presence of SWNT.−, detected by laser flash photolysis, was confirmed by time-resolved pulse radiolysis and steady-state bulk electrolysis. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2003/z51289_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Prime rule-based methodologies give inadequate control (1986)

The use of rule-based methodologies in the development of Expert Systems is widespread. In order to provide good explanations in these systems it is desirable that the rules be prime. The difficulty of expressing control in such rules, and thus arriving at a desirable sequencing of events, has led to pragmatic additions to the basic methodology. Recent developments in the theory of decision processes have provided new insight into the form of a desirable sequencing. Prime rules, even when augmented by sophisticated control strategies, cannot generate from backward chaining all these desirable sequencings. Furthermore, if one of these desirable sequencings happens to be generated from prime rules it may be by luck rather than design.
PET of Malignant Melanoma Using <sup>18</sup>F-Labeled Metallopeptides (2009)

Specific in vivo targeting of (18)F-FB-RMSH-1 to malignant melanoma was successfully achieved in preclinical models with high MC1R expression. Thus, the radiofluorinated metallopeptide (18)F-FB-RMSH-1 is a promising molecular probe for PET of MC1R-positive tumors.
Optoelectronic feedback loop for intensity noisesuppression in a high bit rate FM-operated Er-Yb laser (1999)

A simple technique, based on a double optoelectronic feedback loop, is proposed and demonstrated to suppress intensity noise in high bit rate pulse trains generated by a frequency-modulated Er-Yb:glass laser. Relaxation oscillation and frequency detuning noise components are reduced by 18.3 and 33 dB, respectively, achieving residual amplitude fluctuations of < 0.9%.