gebhart/patrepeval huggingface dataset:
Localized reactive badger culling increases risk of bovine tuberculosis in nearby cattle herds (2011)

Human and livestock diseases can be difficult to control where infection persists in wildlife populations. Control of bovine tuberculosis (bTB) in British cattle is complicated by the maintenance of Mycobacterium bovis (the causative agent of bTB) in badgers, acting as reservoirs of infection. Although over 20 000 badgers were culled to control bTB between 1975 and 1997, the incidence of bTB in cattle has substantially increased in parts of Great Britain in recent decades. Our case-control study, involving 1208 cattle herds, provides further evidence of the detrimental effect of localized reactive badger culling in response to the disclosure of a confirmed bTB herd breakdown in cattle. The presence of any reactive badger culling activity and increased numbers of badgers culled in the vicinity of a herd were associated with significantly increased bTB risk, even after adjusting for other important local risk factors. Such findings may partly explain why some earlier localized approaches to bTB control were ineffective.
Phase 1 Safety, Pharmacokinetics, and Pharmacodynamics of Dapivirine and Maraviroc Vaginal Rings (2015)

In this first study of a combination microbicide vaginal ring, all 4 rings were safe and well tolerated. Tissue DPV concentrations were 1000 times greater than plasma concentrations and single drug rings had more stable pharmacokinetics. DPV, but not MVC, demonstrated concentration-dependent inhibition of HIV-1 infection in cervical tissue. Because MVC concentrations were consistently detectable only in CVF and not in plasma, improved drug release of MVC rings is needed.
[Safety of new anti-arrhythmic drugs]. (2005)

The majority of new antiarrhythmic drugs are still undergoing clinical trials and are not yet available on the French market. The studies of efficacy are mainly targeted on the treatment of atrial fibrillation. Intravenous ibutilide prolongs the duration of the action potential by stimulating sodium exchange during phase 2 of the action potential. Used for terminating episodes of atrial flutter and fibrillation, ibutilide has been shown to have a low proarrhythmic effect. Dofetilide is a pure I(Kr) current antagonist and is given orally. The molecule prolongs the duration of the atrial and ventricular action potentials. The amplitude of this effect is inversely related to the heart rate. No effect has been observed on the mortality rate in the post-infarct period. Adjusting the dosage with respect to renal function has reduced the occurrence of torsades de pointe from 4.8 to 2.9%. Azimilide is an I(Kr) and I(Ks) current blocker and its efficacy decreases at rapid heart rates. After oral administration, azimilide does not appear to have a deleterious effect in patients with a history of myocardial infarction and left ventricular dysfunction. The risk of torsades de pointe is less than 1%. Cases of neutropaenia have been reported. Dronedarone is an amiodarone analogue without iodine. The molecule prolongs atrial and ventricular action potentials and its efficacy is maintained at high heart rates. This drug had deleterious effects when given to patients with coronary artery disease and left ventricular dysfunction. Gastrointestinal side effects may be observed at high dosage. The great advantages of dronedarone are the absence of thyroid complications and of pro-arrhythmic effects.
Single‐scan rest/stress imaging <sup>18</sup>F‐labeled flow tracers (2012)

Detailed simulations predict that accurate and precise rest-stress blood flow measurements can be obtained in 20-30 min.
802.11n under the microscope (2008)

We present an experimental study of IEEE 802.11n (high throughput extension to the 802.11 standard) using commodity wireless hardware. 802.11n introduces a variety of new mechanisms including physical layer diversity techniques, channel bonding and frame aggregation mechanisms. Using measurements from our testbed, we analyze the fundamental characteristics of 802.11n links and quantify the gains of each mechanism under diverse scenarios. We show that the throughput of an 802.11n link can be severely degraded (up ≈85%) in presence of an 802.11g link. Our results also indicate that increased amount of interference due to wider channel bandwidths can lead to throughput degradation. To this end, we characterize the nature of interference due to variable channel widths in 802.11n and show that careful modeling of interference is imperative in such scenarios. Further, as a reappraisal of previous work, we evaluate the effectiveness of MAC level diversity in the presence of physical layer diversity mechanisms introduced by 802.11n.
A 1.9 GHZ High Efficiency Class-F SOI CMOS Power Amplifier (2015)

In this paper, a 1.9 GHz high efficiency power amplifier (PA) for wireless applications is present. The PA is a two stage class-F power amplifier. The drive stage is self-biased cascode structure and the output stage is common source. The PA is designed and simulated in 0.18 m silicon-on-insulator (SOI) technology. The simulation results show that at 1.9 GHz, this class-F PA can achieve 50 % PAE (power added efficiency) at (1dB compression point) output power of 25 dBm and the linear gain is 20 dB.
The RNA binding domain of ribosomal protein L11: three-dimensional structure of the RNA-bound form of the protein and its interaction with 23 S rRNA (1997)

The three-dimensional solution structure has been determined by NMR spectroscopy of the 75 residue C-terminal domain of ribosomal protein L11 (L11-C76) in its RNA-bound state. L11-C76 recognizes and binds tightly to a highly conserved 58 nucleotide domain of 23 S ribosomal RNA, whose secondary structure consists of three helical stems and a central junction loop. The NMR data reveal that the conserved structural core of the protein, which consists of a bundle of three alpha-helices and a two-stranded parallel beta-sheet four residues in length, is nearly the same as the solution structure determined for the non-liganded form of the protein. There are however, substantial chemical shift perturbations which accompany RNA binding, the largest of which map onto an extended loop which bridges the C-terminal end of alpha-helix 1 and the first strand of parallel beta-sheet. Substantial shift perturbations are also observed in the N-terminal end of alpha-helix 1, the intervening loop that bridges helices 2 and 3, and alpha-helix 3. The four contact regions identified by the shift perturbation data also displayed protein-RNA NOEs, as identified by isotope-filtered three-dimensional NOE spectroscopy. The shift perturbation and NOE data not only implicate helix 3 as playing an important role in RNA binding, but also indicate that regions flanking helix 3 are involved as well. Loop 1 is of particular interest as it was found to be flexible and disordered for L11-C76 free in solution, but not in the RNA-bound form of the protein, where it appears rigid and adopts a specific conformation as a result of its direct contact to RNA.
Homocysteine inhibits endothelial cell growth via DNA hypomethylation of the cyclin Agene (2007)

We reported previously that homocysteine (Hcy) inhibits endothelial cell (EC) growth by transcriptional inhibition of the cyclin A gene via a hypomethylation-related mechanism. In this study, we examined the effect of Hcy on epigenetic modification of the cyclin A gene and its biologic role in human ECs. Cyclin A mRNA levels were significantly suppressed by Hcy and a DNA methyltransferase inhibitor. The cyclin A promoter contains a CpG island spanning a 477-bp region (-277/200). Bisulfite sequencing followed by polymerase chain reaction (PCR) amplification of the cyclin A promoter (-267/37) showed that Hcy eliminated methylation at 2 CpG sites in the cyclin A promoter, one of which is located on the cycle-dependent element (CDE). Mutation of CG sequence on the CDE leads to a 6-fold increase in promoter activity. Hcy inhibited DNA methyltransferase 1 (DNMT1) activity by 30%, and reduced the binding of methyl CpG binding protein 2 (MeCP2) and increased the bindings of acetylated histone H3 and H4 in the cyclin A promoter. Finally, adenovirus-transduced DNMT1 gene expression reversed the inhibitory effect of Hcy on cyclin A expression and EC growth inhibition. In conclusion, Hcy inhibits cyclin A transcription and cell growth by inhibiting DNA methylation through suppression of DNMT1 in ECs.
An epistemological framework for medical knowledge-based systems (1992)

An abstraction paradigm for unifying different perspectives concerning the analysis and design of knowledge-based systems (KBSs) is presented. The model accounts for all of the conceptual features of knowledge-based systems, thus making clear which features are intrinsic to the problem and which are artifacts of the implementation. The proposal is based on a two-level analysis of knowledge-based systems: an epistemological and a computational level. At the first level, ontology and inference models of a knowledge-based system are defined. At the computational level, methods and formalisms are adopted after the epistemological analysis has been carried out. The study is confined to medicine with three generic tasks identified: diagnosis, therapy planning, and monitoring. The results of this analysis indicate that the generic tasks manage different ontologies, but can be executed exploiting a unique inference model. Computational issues are discussed to argue that the model provides a conceptual view on existing systems and some design insights for future ones.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Enhancement of<scp>l</scp>-ornithine production by disruption of three genes encoding putative oxidoreductases in<i>Corynebacterium glutamicum</i> (2014)

Recently, Corynebacterium glutamicum has been shown to exhibit gluconate bypass activity, with two key enzymes, glucose dehydrogenase (GDH) and gluconate kinase, that provides an alternate route to 6-phosphogluconate formation. In this study, gene disruption analysis was used to examine possible metabolic functions of three proteins encoded by open reading frames having significant sequence similarity to GDH of Bacillus subtilis. Chromosomal in-frame deletion of three genes (NCgl0281, NCgl2582, and NCgl2053) encoding putative NADP⁺-dependent oxidoreductases led to the absence of GDH activity and correlated with increased specific glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase activities. This finding suggested that enhanced carbon flux from glucose was directed toward the oxidative pentose phosphate (PP) pathway, when the mutant was cultivated with 6 % glucose. Consequently, the mutant showed 72.4 % increased intracellular NADPH and 66.3 % increased extracellular L-ornithine production. The enhanced activities of the oxidative PP pathway in the mutant explain both the increased intracellular NADPH and the high extracellular concentration of L-ornithine. Thus, the observed metabolic changes in this work corroborate the importance of NADPH in L-ornithine production from C. glutamicum.
Demand-adaptive Clothing Image Retrieval Using Hybrid Topic Model (2016)

This paper proposes a novel approach to meet users' multi-dimensional requirements in clothing image retrieval. It enables users to add search conditions by modifying the color, texture, shape and attribute descriptors of the query images to further refine their requirements. We propose the Hybrid Topic (HT) model to learn the intricate semantic representation of the descriptors above. The model provides an effective multi-dimensional representation of clothes and is able to perform automatic image annotation by probabilistic reasoning from image search. Furthermore, we develop a demand-adaptive retrieval strategy which refines users' specific requirements and removes users' unwanted features. Our experiments show that the HT method significantly outperforms the deep neural network methods. The accuracy could be further improved in cooperation with image annotation and demand-adaptive retrieval strategy.
Enhancing Polysaccharide-Mediated Delivery of Nucleic Acids Through Functionalization with Secondary and Tertiary Amines (2008)

Chitosan is a polysaccharide that has generated significant interest as a non-viral gene delivery vehicle due to its cationic and biocompatible characteristics. However, transfection efficiency of chitosan is significantly lower compared to other cationic gene delivery agents, e.g. polyethyleneimine (PEI), dendrimers or cationic lipids. This is primarily attributed to its minimal solubility and low buffering capacity at physiological pH leading to poor endosomal escape of the gene carrier and inefficient cytoplasmic decoupling of the complexed nucleic acid. Here we have developed an imidazole acetic acid (IAA)-modified chitosan to introduce secondary and tertiary amines to the polymer in order to improve its endosomal buffering and solubility. The modified polymer was characterized by ninhydrin and (1)H NMR assays for degree of modification, while buffering and solubility were analyzed by acid titration. Nanocomplex formation, studied at various polymer-nucleic acid ratios, showed an increase in particle zeta potential for chitosan-IAA, as well as an increase in the effective diameter. Up to 100-fold increase in transfection efficiency of pDNA was seen for chitosan-IAA as compared to native chitosan, nearly matching that of PEI. In addition, transfection of siRNA by the modified polymers showed efficient gene knockdown equivalent to commercially available siPORT Amines. Collectively, these results demonstrate the potential of the imidazole-grafted chitosan as a biocompatible and effective delivery vehicle for both pDNA and siRNA.
In vitro and in vivo assessment of the abuse potential of PF614, a novel BIO-MD™ prodrug of oxycodone (2017)

The Bio-Activated Molecular Delivery prodrug design limits the use of PF614 to the intended oral route of delivery with reduced potential for IV or nasal abuse, as it cannot be activated intravenously or nasally to provide an active opioid. Unlike existing opioid formulations, the extended-release profile of PF614 cannot be accelerated by chewing or ex vivo extraction to pharmacologically active substances.
Characterization of Carotenoid High-Producing<i>Capsicum annuum</i>Cultivars Selected for Paprika Production (2002)

Twelve selected pepper (Capsicum annuum L.) cultivars, bred for mechanical harvesting (grouped ripeness) and adaptation to different cultivation cycles (short to long), have been characterized by their carotenoid pigment content and composition with the aim of producing high-quality paprika. A detailed analysis of the carotenogenesis was performed throughout the ripening process, but with special emphasis on the ripe stage, with the aim of selecting the best cultivar for paprika production. The MA1 cultivar (with grouped ripeness and very short cultivation cycle) showed the highest carotenoid content (12697.58 mg/kg dwt), followed by DN5 and RN2 cultivars with 11086.88 and 10393.29 mg/kg dwt, respectively. Most of the cultivars (MA3, RN1, LR2, LR7, DN3, DR6, Datler, and Mulato) showed a total carotenoid content in the range of 7000-9700 mg/kg dwt. In general, chlorophyll-retaining character was related to high carotenoid content (cultivars DN3, DN5, MA3, Mulato, RN1, and RN2). The general trend of the cultivation cycle was that the shorter the cycle, the higher the total carotenoid content (as exemplified by the cultivar MA1). The lowest total carotenoid content was found for the RR1 cultivar (4856.77 mg/kg dwt), which showed the longest cultivation cycle. Carotenogenic capacity of the cultivars has been discussed relative to total carotenoid content and the R/Y and Caps/Zeax ratios, the main quality traits for breeding cultivars for production of high-quality paprika. The cultivar MA1, with the highest total carotenoid content, high R/Y (2.11) ratio, and highest Caps/Zeax (9.85) ratio, was found to be the most suitable cultivar for paprika production in terms of carotenoid pigment biosynthesis capacity. Moreover, this cultivar has a short cultivation cycle and grouped ripeness, which are both important characteristics for a proper application of mechanical harvesting. The potential improvement of other varieties is also discussed.
Examining Type 1 Diabetes Mathematical Models Using Experimental Data (2022)

Type 1 diabetes requires treatment with insulin injections and monitoring glucose levels in affected individuals. We explored the utility of two mathematical models in predicting glucose concentration levels in type 1 diabetic mice and determined disease pathways. We adapted two mathematical models, one with β-cells and the other with no β-cell component to determine their capability in predicting glucose concentration and determine type 1 diabetes pathways using published glucose concentration data for four groups of experimental mice. The groups of mice were numbered Mice Group 1-4, depending on the diabetes severity of each group, with severity increasing from group 1-4. A Markov Chain Monte Carlo method based on a Bayesian framework was used to fit the model to determine the best model structure. Akaike information criteria (AIC) and Bayesian information criteria (BIC) approaches were used to assess the best model structure for type 1 diabetes. In fitting the model with no β-cells to glucose level data, we varied insulin absorption rate and insulin clearance rate. However, the model with β-cells required more parameters to match the data and we fitted the β-cell glucose tolerance factor, whole body insulin clearance rate, glucose production rate, and glucose clearance rate. Fitting the models to the blood glucose concentration level gave the least difference in AIC of 1.2, and a difference in BIC of 0.12 for Mice Group 4. The estimated AIC and BIC values were highest for Mice Group 1 than all other mice groups. The models gave substantial differences in AIC and BIC values for Mice Groups 1-3 ranging from 2.10 to 4.05. Our results suggest that the model without β-cells provides a more suitable structure for modelling type 1 diabetes and predicting blood glucose concentration for hypoglycaemic episodes.
Estimating reliability and throughput of source-synchronous wave-pipelined interconnect (2009)

Wave pipelining has gained attention for NoC interconnect by its promise of high bandwidth using simple circuits. Reliability issues must be addressed before wave pipelining can be used in practice; so, we develop a statistical model of dynamic timing uncertainty. We show that it is important to distinguish between static and dynamic sources of timing uncertainty, because source-synchronous wave pipelining is much more sensitive to the latter. We use HSPICE simulations to develop a model for a wave pipelined link in a 65 nm CMOS process and apply a statistical approach to determine the achievable throughput at acceptable bit-error rates. Reliability estimates show that a modest amount of dynamic noise can cut achievable throughput in half for a ten-stage wave-pipelined link, and will further degrade longer links. After accounting for noise, traditional globally synchronous design is shown to offer higher throughput than the wave-pipelined design.
Changes in messenger rna and protein levels of proteoglycans and link protein in human osteoarthritic cartilage samples (1997)

The expression of matrix components was increased in chondrocytes of OA cartilage, especially the expression of small proteoglycans, most likely due to the repair processes. A discoordinate gene expression accompanied with imbalanced accumulation of noncollagenous matrix components may contribute to the disorganization of the cartilage and the development of OA processes.
Preclinical studies of ropivacaine extended-release from a temperature responsive hydrogel for prolonged relief of pain at the surgical wound (2019)

Postoperative pain is a common form of acute pain that has been treated commonly by local anesthetics through regional nerve blocking. In this study, a series of experiments were conducted using rats to investigate the pharmacokinetic, distribution, and efficacy of a temperature responsive hydrogel-based drug delivery device (PF-72) containing ropivacaine (0.75%) for extended relief of postoperative pain by allowing the prolonged release of ropivacaine. When the ropivacaine was administered using PF-72, its concentration-time curve (AUC<sub>last</sub>) and peak concentration (C<sub>max</sub>) were 577.0 h*ng/mL and 271.9 ng/mL, respectively. In contrast when the ropivacaine solution was administered using saline solution, its AUC<sub>last</sub> and C<sub>max</sub> were 982.8 h*ng/mL and 423.6 ng/mL, respectively. In the tissue distribution study, the peak concentration and mean area under the curve of the ropivacaine in injection area (target tissue) were found about 2-fold higher in the case of PF-72 compared with the case of conventional ropivacaine solution. These results clearly demonstrate the capability of PF-72 hydrogel to retain the ropivacaine at the injection site for an extended period. Effective extended (at least 24 h) pain relief of ropivacaine administered using PF-72 was found in the pharmacodynamic study of prolonged analgesic effect. The results of this study indicated that local drug delivery by PF-72 hydrogel formulation may be an effective method to achieve extended relief of pain. Other advantages of ropivacaine administration using PF-72 include reduced systemic side effects and high localization of a drug in target tissues.
Condensing Heat Exchanger Microbial Growth and Control Study-Phase II (1993)

<div class="htmlview paragraph">The Space Station Temperature and Humidity Control Condensing Heat Exchangers will be utilized to collect and remove atmospheric water vapor generated by the metabolic and hygienic activity of crew members. The porous hydrophilic coating within the heat exchangers will always be wet. Cabin air will continuously flow through the heat exchangers during system operation which makes them a potential site for microbial colonization.</div> <div class="htmlview paragraph">This paper summarizes the findings from an ongoing study which evaluates biofilm formation on wet hydrophilic coated panels compared to panels to which microbial control measures have been applied. The control measures evaluated are an antimicrobial agent within the coating and periodic drying.</div>