gebhart/patrepeval huggingface dataset:
Mitochonic Acid 5 (MA-5) Facilitates ATP Synthase Oligomerization and Cell Survival in Various Mitochondrial Diseases (2017)

Mitochondrial dysfunction increases oxidative stress and depletes ATP in a variety of disorders. Several antioxidant therapies and drugs affecting mitochondrial biogenesis are undergoing investigation, although not all of them have demonstrated favorable effects in the clinic. We recently reported a therapeutic mitochondrial drug mitochonic acid MA-5 (Tohoku J. Exp. Med., 2015). MA-5 increased ATP, rescued mitochondrial disease fibroblasts and prolonged the life span of the disease model "Mitomouse" (JASN, 2016). To investigate the potential of MA-5 on various mitochondrial diseases, we collected 25 cases of fibroblasts from various genetic mutations and cell protective effect of MA-5 and the ATP producing mechanism was examined. 24 out of the 25 patient fibroblasts (96%) were responded to MA-5. Under oxidative stress condition, the GDF-15 was increased and this increase was significantly abrogated by MA-5. The serum GDF-15 elevated in Mitomouse was likewise reduced by MA-5. MA-5 facilitates mitochondrial ATP production and reduces ROS independent of ETC by facilitating ATP synthase oligomerization and supercomplex formation with mitofilin/Mic60. MA-5 reduced mitochondria fragmentation, restores crista shape and dynamics. MA-5 has potential as a drug for the treatment of various mitochondrial diseases. The diagnostic use of GDF-15 will be also useful in a forthcoming MA-5 clinical trial.
Novel technique to detect rancidity of oils using microwave microstrip ring resonator (2002)

Abstract The present article presents a novel technique to study the rancidity of oils at microwave frequencies through measurements of microwave dielectric properties of liquids. CaCO 3 (chalk) was used as a noninteracting medium (matrix) to hold the liquids within and is used to perturb the microwave microstrip ring resonator (MMRR). The rancidified oils exhibit high dielectric constants, giving higher changes in resonating frequencies. The method is found to be repeatable. Coconut oil ( Cocos nucifera ), sunflower oil ( Helianthus annuus ), rancidified coconut oil and rancidified sunflower oil were studied using this technique. The resonant frequency was measured using a scalar network analyzer (SNA, HP 8757C). The method shows noticeable differences in resonating frequency (few tens of MHz) between fresh oil and rancidified oil. © 2002 Wiley Periodicals, Inc. Microwave Opt Technol Lett 33: 377–379, 2002; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/mop.10327
Improving the Reliability of a FPGA Using Fault-Tolerance Mechanism Based on Magnetic Memory (MRAM) (2010)

The current SRAM based FPGA, are more and more susceptible to Single Event Upset (SEU) due to Neutron particle interference. The problem is exasperated reducing the CMOS submicronic scale in the manufacturing process, specially for the next generation of SRAM-based FPGAs. Nowadays is common practice for SRAM manufactories to embed fault tolerant mechanisms like Error-Correcting Code schemes in SRAM memory banks for CMOS technology below 90 nm, to mitigate SEU. The present work proposes an approach to improve the reliability of the FPGAs, regarding SEU events at ground level for the future submicronic scale technologies proposing the adoption of Magnetic Random Access Memories (MRAMs) cells into a simple fault-tolerant system for FPGAs manufactured below 65 nm submicronic scale.
RADIUS Attributes for Tunnel Protocol Support (2000)

This document defines a set of RADIUS attributes designed to support the provision of compulsory tunneling in dial-up networks.
A flit-level speedup scheme for network-on-chips using self-reconfigurable bi-directional channels (2012)

In this work, we propose a flit-level speedup scheme to enhance the network-on-chip(NoC) performance utilizing bidirectional channels. In addition to the traditional efforts on allowing flits of different packets using the idling internal and external bandwidth of the bi-directional channel, our proposed flit-level speedup scheme also allows flits within the same packet to be transmitted simultaneously on the bi-directional channel. For inter-router transmission, a novel distributed channel configuration protocol is developed to dynamically control the link directions. For the intra-router transmission, an input buffer architecture which supports reading and writing two flits from the same virtual channel at the same time is proposed. The switch allocator is also designed to support flit-level parallel arbitration. Simulation results on both synthetic traffic and real benchmarks show performance improvement in throughput and latency over the existing architectures using bi-directional channels.
Opossum serum .alpha.1-proteinase inhibitor: Purification, linear sequence, and resistance to inactivation by rattlesnake venom metalloproteinase (1993)

Opossum (Didelphis virginiana) serum was fractionated with (NH4)2SO4 and then chromatographed on DEAE-Sepharose and phenyl-Sepharose. Affinity chromatography on a protein A-Sepharose-antibody column removed traces of opossum serum metalloproteinase inhibitors, and resulted in a homogeneous preparation of opossum alpha 1-proteinase inhibitor (alpha 1-PI). The inhibitor is a single-chain glycoprotein (17.7% carbohydrate) with an estimated M(r) = 54,000. An opossum liver cDNA library was immunoscreened, and clones containing cDNA encoding for the open reading frame for opossum alpha 1-PI were isolated. The cDNA inserts contained nucleotide sequences corresponding to the amino-terminal and an internal peptide sequence of opossum alpha 1-PI which had been separately determined by protein sequence analysis. The entire inserts coded for a protein consisting of a 21-residue signal peptide and a 389-residue mature protein. Opossum alpha 1-PI shows 51-58% identity with other mammalian alpha 1-PI amino acid sequences, and the conserved residues expected for a member for the serpin family have been retained. The carbohydrate attachment sites and the reactive site residues (M-S) of opossum alpha 1-PI are identical to those of human alpha 1-PI. Opossum alpha 1-PI formed stable enzyme/inhibitor complexes with trypsin, chymotrypsin, and human neutrophil elastase, but did not react with thrombin or with snake venom serine proteinases. Opossum alpha 1-PI was inactivated by papain or Pseudomonas aeruginosa elastase, and electrophoretic analysis of the reaction products indicated limited proteolysis in the reactive site loop of the inhibitor.(ABSTRACT TRUNCATED AT 250 WORDS)
한국어판 신체적 자기효능감 척도의 문항 및 범주기능 검사 (2009)

이 연구에서는 윤영철(2008)에 의해 재번역되어 제시된 한국어판 신체적 자기효능감 척도(PSES-K)의 문항적합도와 반응척도의 범주기능을 Rasch 평정척도 모형을 적용하여 검사하였다. WINSTEPS(ver. 3.33) 프로그램을 이용한 Rasch 분석 결과, 18번 문항의 적합도가 기준값인 1.5를 초과하여 과도한 다양성을 갖는 것으로 나타났다. 해당 문항을 삭제한 후 실시한 범주기능 검사에서 수준에 따라 서열화 되지 않은 임계값이 있었으며, 문제가 있는 범주와 인접한 범주를 여러 개 조합으로 병합하여 비교한 결과, 범주3과 4를 병합한 5점 척도가 가장 적합한 것으로 나타났다. 별도의 응답자로부터 수집된 이차자료를 동일한 절차로 분석하여 앞선 분석에서 얻어진 결과가 재현되는지 확인하였다. 이차자료 분석 결과, 18번 문항의 적합도가 기준범위를 넘어서고, 임계값이 크기순으로 배열되지 않았으며 범주3과 4의 병합이 적합한 것으로 나타남으로써 두 자료의 분석결과가 동일하였다. 단일선형척도 위에서 응답자의 수준과 문항난이도를 비교한 결과, 신체적 자기효능감 수준 1.0 로지트 이상의 응답자를 변별할 수 있는 문항의 추가가 필요한 것으로 나타났다.
Some considerations about the church of Santa Caterina of Barcelona (1996)

Una nova lectura de les informacions que posseem del convent de Santa Caterina de Barcelona, l'aportaci de noves dades i un estudi comparatiu amb les fbriques mendicants contempornies permeten proposar l'existncia d'una fbrica d'arcs diafragmtics, precedent a l'esglsia gtica, que, sense grans canvis, perdur fins al segle XIX. Fet que significa haver de modificar la dataci de l'esglsia gtica, tot avanant-la fins al segle XIV, fruit d'una campanya de millores que substituiria l'esglsia aixecada al segle XIII.
Transcriptional regulatory elements stimulate recombination in extrachromosomal substrates carrying immunoglobulin switch-region sequences. (1992)

We have developed a sensitive genetic assay to analyze DNA sequences and regulatory elements required for immunoglobulin heavy chain isotype switch recombination. Recombination substrates containing mu and gamma 3 chain switch (S)-region sequences, S mu and S gamma 3, are transiently introduced into primary murine B cells cultured with lipopolysaccharide to induce isotype switching. Recombination involving S-region sequences deletes a conditionally lethal marker, the leftward promoter of phage lambda (lambda PL), enabling recovered plasmids to transform Escherichia coli. In substrates carrying S mu-lambda PL-S gamma 3, about 2% of replicated molecules undergo deletion of lambda PL during transfection; insertion of either the immunoglobulin heavy chain promoter and enhancer sequences or cytomegalovirus IE1 promoter region upstream of S mu increases recombination 10-fold or more to 25% of replicated molecules. Guanosine-rich S-region sequences are essential for efficient recombination of these substrates.
Electromagnetic reprogrammable coding-metasurface holograms (2017)

Metasurfaces have enabled a plethora of emerging functions within an ultrathin dimension, paving way towards flat and highly integrated photonic devices. Despite the rapid progress in this area, simultaneous realization of reconfigurability, high efficiency, and full control over the phase and amplitude of scattered light is posing a great challenge. Here, we try to tackle this challenge by introducing the concept of a reprogrammable hologram based on 1-bit coding metasurfaces. The state of each unit cell of the coding metasurface can be switched between '1' and '0' by electrically controlling the loaded diodes. Our proof-of-concept experiments show that multiple desired holographic images can be realized in real time with only a single coding metasurface. The proposed reprogrammable hologram may be a key in enabling future intelligent devices with reconfigurable and programmable functionalities that may lead to advances in a variety of applications such as microscopy, display, security, data storage, and information processing.Realizing metasurfaces with reconfigurability, high efficiency, and control over phase and amplitude is a challenge. Here, Li et al. introduce a reprogrammable hologram based on a 1-bit coding metasurface, where the state of each unit cell of the coding metasurface can be switched electrically.
Patterns of maladjustment among mentally retarded children and youth. (1986)

Data from factor analysis of rating scale evaluations of teachers as a basis for classifying the patterns of emotional and behavioral problems found in EMR students in the public schools were presented. These factors permitted a contrast between EMR elementary- and secondary-school-level boys and girls and a comparison group of nonretarded students. Implications for an understanding of the emotional and behavioral characteristics of students identified as EMR and for related programming needs were discussed.
An Integrated Analog Optical Motion Sensor (1986)

This paper describes the theory and implementation of an integrated system that\nreports the uniform motion of a visual scene. We have built a VLSI circuit that\nreports the motion of an image focused directly on it. The chip contains an integrated\nphotosensor array to sense the image and has closely coupled custom circuits to perform\ncomputation and data extraction.
Magnesium is required for specific DNA binding of the CREB B-ZIP domain (2002)

We have examined binding of the CREB B-ZIP protein domain to double-stranded DNA containing a consensus CRE sequence (5'-TGACGTCA-3'), the related PAR, C/EBP and AP-1 sequences and the unrelated SP1 sequence. DNA binding was assayed in the presence or absence of MgCl2 and/or KCl using two methods: circular dichroism (CD) spectroscopy and electrophoretic mobility shift assay (EMSA). The CD assay allows us to measure equilibrium binding in solution. Thermal denaturation in 150 mM KCl indicates that the CREB B-ZIP domain binds all the DNA sequences, with highest affinity for the CRE site, followed by the PAR (5'-TAACGTTA-3'), C/EBP (5'-TTGCGCAA-3') and AP-1 (5'-TGAGTCA-3') sites. The addition of 10 mM MgCl2 diminished DNA binding to the CRE and PAR DNA sequences and abolished binding to the C/EBP and AP-1 DNA sequences, resulting in more sequence-specific DNA binding. Using 'standard' EMSA conditions (0.25x TBE), CREB bound all the DNA sequences examined. The CREB-CRE complex had an apparent Kd of approximately 300 pM, PAR of approximately 1 nM, C/EBP and AP-1 of approximately 3 nM and SP1 of approximately 30 nM. The addition of 10 mM MgCl2 to the polyacrylamide gel dramatically altered sequence-specific DNA binding. CREB binding affinity for CRE DNA decreased 3-fold, but binding to the other DNA sequences decreased >1000-fold. In the EMSA, addition of 150 mM KCl to the gels had an effect similar to MgCl2. The magnesium concentration needed to prevent non-specific electrostatic interactions between CREB and DNA in solution is in the physiological range and thus changes in magnesium concentration may be a cellular signal that regulates gene expression.
Poly(A)-Binding Protein Facilitates Translation of an Uncapped/Nonpolyadenylated Viral RNA by Binding to the 3′ Untranslated Region (2012)

Viruses employ an alternative translation mechanism to exploit cellular resources at the expense of host mRNAs and to allow preferential translation. Plant RNA viruses often lack both a 5' cap and a 3' poly(A) tail in their genomic RNAs. Instead, cap-independent translation enhancer elements (CITEs) located in the 3' untranslated region (UTR) mediate their translation. Although eukaryotic translation initiation factors (eIFs) or ribosomes have been shown to bind to the 3'CITEs, our knowledge is still limited for the mechanism, especially for cellular factors. Here, we searched for cellular factors that stimulate the 3'CITE-mediated translation of Red clover necrotic mosaic virus (RCNMV) RNA1 using RNA aptamer-based one-step affinity chromatography, followed by mass spectrometry analysis. We identified the poly(A)-binding protein (PABP) as one of the key players in the 3'CITE-mediated translation of RCNMV RNA1. We found that PABP binds to an A-rich sequence (ARS) in the viral 3' UTR. The ARS is conserved among dianthoviruses. Mutagenesis and a tethering assay revealed that the PABP-ARS interaction stimulates 3'CITE-mediated translation of RCNMV RNA1. We also found that both the ARS and 3'CITE are important for the recruitment of the plant eIF4F and eIFiso4F factors to the 3' UTR and of the 40S ribosomal subunit to the viral mRNA. Our results suggest that dianthoviruses have evolved the ARS and 3'CITE as substitutes for the 3' poly(A) tail and the 5' cap of eukaryotic mRNAs for the efficient recruitment of eIFs, PABP, and ribosomes to the uncapped/nonpolyadenylated viral mRNA.
CD26: A Multifunctional Integral Membrane and Secreted Protein of Activated Lymphocytes (2001)

CD26 has proved interesting in the fields of immunology, endocrinology, cancer biology and nutrition owing to its ubiquitous and unusual enzyme activity. This dipeptidyl aminopeptidase (DPP IV) activity generally inactivates but sometimes alters or enhances the biological activities of its peptide substrates, which include several chemokines. CD26 costimulates both the CD3 and the CD2 dependent T-cell activation and tyrosine phosphorylation of TCR/CD3 signal transduction pathway proteins. CD26 in vivo has integral membrane protein and soluble forms. Soluble CD26 is at significant levels in serum, these levels alter in many diseases and soluble CD26 can modulate in vitro T-cell proliferation. CD26, being an adenosine deaminase binding protein (ADAbp), functions as a receptor for ADA on lymphocytes. The focus of this review is the structure and function of CD26 and the influence of its ligand binding activity on T-cell proliferation and the T cell costimulatory activity of CD26.
Clinical laboratory and SARS-CoV-2 infection: where do we stand? (2020)

This document mentions serology testing, namely assays to detect SARS-CoV-2 specific antibodies, as being one of the next research priorities While some assays have already become available, several topics are under investigation and discussion on these fields: (1) dynamic of immunological response, (2) development and validation of useful serological assays, (3) comparative studies of available molecular and serological assays, and (4) the eventual role of antibodies on protective immunity Out of urgency on focusing on etiologic diagnosis and the paucity of systematic and reliable data, much less attention has been devoted so far to the potential role of other diagnostic assays in the management of patients with COVID-19, i e the severe form of viral pneumonia caused by SARS-CoV-2 Some early reports have started to shed some light on this;the following sections will detail with the main findings In conclusion, while the etiological diagnosis of COVID-19 has already been established within a very short time from the recognition of this new clinical entity, progress on the role of known and new diagnostic biomarkers is still needed to provide guidance to clinicians and help laboratory professionals establish the right value and relevance of those on the diagnosis, prognosis and monitoring of SARS-CoV-2 infection
Hierarchical Modeling of a Tabu Search Based Manufacturing Plant Order Sequencing and Vehicle Dispatching Problem (2003)

With the rapid advance of manufacturing technology and the increasing use of call centers in industry, it is very common nowadays for customers to expect their goods to be delivered within hours or specified time windows by online ordering. For a zero inventory company, that means the cycle time from receiving orders to production and delivery the finished goods to customers has to match with customer’s request. The purpose of this paper is to present a hierarchical modeling approach for such kind of just-in-time systems. It shows that such a problem could be mapped into a variant of Vehicle Routing Problems and based on which, a Tabu Search based heuristic method is proposed to obtain improved solutions upon an initial solution.
Definition and analysis of hardware- and software-fault-tolerant architectures (1990)

A structured definition of hardware- and software-fault-tolerant architectures is presented. Software-fault-tolerance methods are discussed, resulting in definitions for soft and solid faults. A soft software fault has a negligible likelihood or recurrence and is recoverable, whereas a solid software fault is recurrent under normal operations or cannot be recovered. A set of hardware- and software-fault-tolerant architectures is presented, and three of them are analyzed and evaluated. Architectures tolerating a single fault and architectures tolerating two consecutive faults are discussed separately. A sidebar addresses the cost issues related to software fault tolerance. The approach taken throughout is as general as possible, dealing with specific classes of faults or techniques only when necessary.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
The Elevated 10-Year Risk of Cervical Precancer and Cancer in Women With Human Papillomavirus (HPV) Type 16 or 18 and the Possible Utility of Type-Specific HPV Testing in Clinical Practice (2005)

HPV screening that distinguishes HPV16 and HPV18 from other oncogenic HPV types may identify women at the greatest risk of > or = CIN3 and may permit less aggressive management of other women with oncogenic HPV infections.
Temporal recruitment of CCAAT/enhancer-binding proteins to early and late adipogenic promoters in vivo (2006)

The CCAAT/enhancer-binding protein (C/EBP) family of transcriptional regulators is critically important for the activation of adipogenic genes during differentiation. The C/EBPbeta and delta isoforms are rapidly induced upon adipocyte differentiation and are responsible for activating the adipogenic regulators C/EBPalpha and peroxisome proliferator activated receptor (PPAR)gamma2, which together activate the majority of genes expressed in differentiating adipocytes. However, mitosis is required following the induction of adipogenesis, and the activation of C/EBPalpha and PPARgamma2 gene expression is delayed until cell division is underway. Previous studies have used electromobility shift assays to suggest that this delay is due, at least in part, to a delay between the induction of C/EBPbeta protein levels and the acquisition of DNA binding capacity by C/EBPbeta. Here we used in vivo chromatin immunoprecipitation analysis of the C/EBPalpha, PPARgamma2, resistin, adiponectin, and leptin promoters to examine the kinetics of C/EBP protein binding to adipogenic genes in differentiating cells. In contrast to prior studies, we determined that C/EBPbeta and delta were bound to endogenous regulatory sequences controlling the expression of these genes within 1-4 h of adipogenic induction. These results indicated that C/EBPbeta and delta bind not only to genes that are induced early in the adipogenic process but also to genes that are induced much later during differentiation, without a delay between induction of C/EBP protein levels and DNA binding by these proteins. We also showed that each of the genes examined undergoes a transition in vivo from early occupancy by C/EBPbeta and delta to occupancy by C/EBPalpha at times that correlate with the induction of C/EBPalpha protein levels, demonstrating the generality of the transition during adipogenesis and indicating that the binding of specific C/EBP isoforms does not correlate with timing of expression from each gene. We have concluded that C/EBP family members bind to adipogenic genes in vivo in a manner that follows the induction of C/EBP protein synthesis.
Multi agent simulation of occupants' presence and behaviour (2011)

To better understand how we can improve urban sustainability we need to focus our predictive efforts at the grassroots level of individual inhabitants. To this end we propose a multi agent simulation platform. In describing each element of this platform we review research advances in modelling occupants ’ presence, activities, behaviours, comfort and investments; suggesting also where further research is required. Our motive here is not to be prescriptive, but simply to start a debate and the process of achieving consensus so that research and development efforts are targeted in a common direction.
Investigaciones en el ushnu mayor de Incahuasi, Cañete: contribuciones al estudio de los ushnus incas (2016)

The archaeological site of Incahuasi, located in the middle valley of the Caete river, in the south-central coast of Peru, has often been mentioned in the archaeological literature; however, studies in the settlement have been superficial to date. Recent research conducted in sector E have shown the constructive complexity of the ushnu in the main square of the site and at least three construction phases associated with changes in size and orientation have been identified. Excavations of the main ushnu show the complex occupation of the site, which was developed during a much longer time than those mentioned in colonial documents.