gebhart/patrepeval huggingface dataset:
Efficacy of Rifampin and Its Combinations with Imipenem, Sulbactam, and Colistin in Experimental Models of Infection Caused by Imipenem-Resistant <i>Acinetobacter baumannii</i> (2010)

There are currently no defined optimal therapies available for multidrug-resistant (MDR) Acinetobacter baumannii infections. We evaluated the efficacy of rifampin, imipenem, sulbactam, colistin, and their combinations against MDR A. baumannii in experimental pneumonia and meningitis models. The bactericidal in vitro activities of rifampin, imipenem, sulbactam, colistin, and their combinations were tested using time-kill curves. Murine pneumonia and rabbit meningitis models were evaluated using the A. baummnnii strain Ab1327 (with MICs for rifampin, imipenem, sulbactam, and colistin of 4, 32, 32, and 0.5 mg/liter, respectively). Mice were treated with the four antimicrobials and their combinations. For the meningitis model, the efficacies of colistin, rifampin and its combinations with imipenem, sulbactam, or colistin, and of imipenem plus sulbactam were assayed. In the pneumonia model, compared to the control group, (i) rifampin alone, (ii) rifampin along with imipenem, sulbactam, or colistin, (iii) colistin, or (iv) imipenem plus sulbactam significantly reduced lung bacterial concentrations (10.6 +/- 0.27 [controls] versus 3.05 +/- 1.91, 2.07 +/- 1.82, 2.41 +/- 1.37, 3.4 +/- 3.07, 6.82 +/- 3.4, and 4.22 +/- 2.72 log(10) CFU/g, respectively [means +/- standard deviations]), increased sterile blood cultures (0% versus 78.6%, 100%, 93.3%, 93.8%, 73.3%, and 50%), and improved survival (0% versus 71.4%, 60%, 46.7%, 43.8%, 40%, and 85.7%). In the meningitis model rifampin alone or rifampin plus colistin reduced cerebrospinal fluid bacterial counts (-2.6 and -4.4 log(10) CFU/ml). Rifampin in monotherapy or with imipenem, sulbactam, or colistin showed efficacy against MDR A. baumannii in experimental models of pneumonia and meningitis. Imipenem or sulbactam may be appropriate for combined treatment when using rifampin.
Prognostic significance of serum antibodies to HPV-16 L1 virus-like particles in patients with invasive cervical cancer (2006)

Antibodies to HPV-16 L1 capsid protein may be of prognostic value for patients with invasive cervical cancer and lack of HPV-16 L1 antibodies may indicate a group of patients with a poor prognosis.
On the flexibility offered by state feedback in multivariable systems beyond closed loop eigenvalue assignment (1976)

A characterization is given for the class of all closed loop eigenvector sets which can be obtained with a given set of distinct closed loop eigenvalues using state feedback. It is shown, furthermore, that the freedom one has in addition to specifying the closed loop eigenvalues is precisely this: to choose one set of closed loop eigenvectors from this class. Included in the proof of this result is an algorithm for computing the matrix of feedback gains which gives the chosen closed loop eigenvalues and eigenvectors. A design scheme based on these results is presented which gives the designer considerable freedom to choose the distribution of the modes among the output components. One interesting feature is that the distribution of a mode among the output components can be varied even if the mode is not controllable.
The effect of dihydroxydibutylether on bile lipid composition in patients with gallstones. (1983)

Dihydroxydibutylether (DHBE, 900 mg/day, in divided doses) was given to 13 patients with radiolucent gallstones. Bile lipid composition and bile saturation were studied before treatment and after 1 and 2 months of therapy. Bile was collected by duodenal intubation (after cerulein-induced gallbladder contraction) in each patient, and biliary lipids were estimated with standard laboratory techniques. The results of this study showed that DHBE treatment was associated with a significant (p less than 0.01) reduction of bile saturation with cholesterol (saturation index: 1.37 +/- 0.39 before treatment, 1.14 +/- 0.35 after 1 month, and 1.08 +/- 0.29 after 2 months). The possible mechanism of this effect and the clinical relevance are discussed.
Hydrogen embrittlement control (1985)

Hydrogen embrittlement in high-strength steels can cause fractures which are unacceptable in the aerospace and aircraft industry. What is needed is a combined effort to minimize the waste created in overlapping or duplicating efforts to solve the problem. Efforts to create effective standards have been thwarted by a lack of support from industry. The cost impact of damage is not appreciated until after a catastrophic event occurs. Large legal settlements in favor of injured parties have led a number of manufacturers to become more concerned and to provide more support for research. Cooperative technical exchanges are producing new testing information that can be incorporated into standards. The transfer of new technology to other industries will follow successful demonstration of embrittlement control technologies. 4 figures.
Roll‐to‐Roll Printed Silver Nanowire Semitransparent Electrodes for Fully Ambient Solution‐Processed Tandem Polymer Solar Cells (2015)

Silver nanowires (AgNWs) and zinc oxide (ZnO) are deposited on flexible substrates using fast roll‐to‐roll (R2R) processing. The AgNW film on polyethylene terephthalate (PET) shows >80% uniform optical transmission in the range of 550–900 nm. This electrode is compared to the previously reported and currently widely produced indium‐tin‐oxide (ITO) replacement comprising polyethylene terephthalate (PET)|silver grid|poly(3,4‐ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS)|ZnO known as Flextrode. The AgNW/ZnO electrode shows higher transmission than Flextrode above 490 nm in the electromagnetic spectrum reaching up to 40% increased transmission at 750 nm in comparison to Flextrode. The functionality of AgNW electrodes is demonstrated in single and tandem polymer solar cells and compared with parallel devices on traditional Flextrode. All layers, apart from the semitransparent electrodes which are large‐scale R2R produced, are fabricated in ambient conditions on a laboratory roll‐coater using printing and coating methods which are directly transferrable to large‐scale R2R processing upon availability of materials. In a single cell structure, Flextrode is preferable with active layers based on poly‐3‐hexylthiophene(P3HT):phenyl‐C61‐butyric acid methylester (PCBM) and donor polymers of similar absorption characteristics while AgNW/ZnO electrodes are more compatible with low band gap polymer‐based single cells. In tandem devices, AgNW/ZnO is more preferable resulting in up to 80% improvement in PCE compared to parallel devices on Flextrode.
12 The Dauer Larva (1988)

I. INTRODUCTION The dauer, or “enduring,” larval stage of Caenorhabditis elegans is an example of facultative diapause. Environmental factors act as signals to a receptive developmental stage (the L1 larva), resulting in altered physiology and developmental potential that leads to formation of a third-stage larva specialized for dispersal and long-term survival. Dauer larvae are capable of active movement, but they do not feed. They have a unique morphology and resistance to stress, are altered in energy metabolism, and are arrested in development, as well as aging. Dauer larvae survive four to eight times the 3-week life span of animals that have bypassed the dauer stage. The dauer state itself has been considered to be nonaging, because the duration of the dauer stage does not affect postdauer life span (Klass and Hirsh 1976). As far as is known, the consumption of stored energy may be the major factor limiting dauer larva life expectancy. Various forms of diapause are common among nematodes, and there may be interesting parallels between the C. elegans dauer larva and arrested developmental forms that have been documented as obligatory stages in the life cycles of a number of parasitic species (for review, see Michel 1974; Evans and Perry 1976). In many cases, these arrested stages are dispersal forms specialized to survive periods between host infections (Riddle and Bird 1985). Dauer larvae exhibit behavior not observed in other stages. Pharyngeal pumping is completely suppressed, and the larvae often lie motionless. Although lethargic, they do show a negative response...
Complete viral RNA genome sequencing of ultra-low copy samples by sequence-independent amplification (2012)

RNA viruses are the causative agents for AIDS, influenza, SARS, and other serious health threats. Development of rapid and broadly applicable methods for complete viral genome sequencing is highly desirable to fully understand all aspects of these infectious agents as well as for surveillance of viral pandemic threats and emerging pathogens. However, traditional viral detection methods rely on prior sequence or antigen knowledge. In this study, we describe sequence-independent amplification for samples containing ultra-low amounts of viral RNA coupled with Illumina sequencing and de novo assembly optimized for viral genomes. With 5 million reads, we capture 96 to 100% of the viral protein coding region of HIV, respiratory syncytial and West Nile viral samples from as little as 100 copies of viral RNA. The methods presented here are scalable to large numbers of samples and capable of generating full or near full length viral genomes from clone and clinical samples with low amounts of viral RNA, without prior sequence information and in the presence of substantial host contamination.
Photostability of aqueous micellar solutions of rhodamines under flash lamp excitation (1986)

The influence of additions with electron-donor and electron-acceptor properties on the lasing properties and photostability of active aqueous micellar solutions of certain rhodamines during their excitation by flash lamps has been studied. A stabilizing influence of ascorbic acid on the lasing parameters of rhodamine solutions under different spectral conditions of excitation has been observed. It was found that in the presence of added substances with electron-acceptor properties (m-dinitrobenzene, cyclooctatetraene) a marked decrease of reversible stimulated absorption in the solution is observed. The experimental conditions were determined under which the aqueous micellar solutions of a series of rhodamines surpassed the alcohol solutions in lasing sufficiency at a high photostability level. Data are cited which characterize the influence of the named additions in the photobleaching of solutions of certain rhodamines under irradiation by flash lamps.
Addition of carboxylic acids to alkynes catalyzed by ruthenium complexes. Vinyl ester formation (1983)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAddition of carboxylic acids to alkynes catalyzed by ruthenium complexes. Vinyl ester formationMichal Rotem and Youval ShvoCite this: Organometallics 1983, 2, 11, 1689–1691Publication Date (Print):November 1, 1983Publication History Published online1 May 2002Published inissue 1 November 1983https://pubs.acs.org/doi/10.1021/om50005a037https://doi.org/10.1021/om50005a037research-articleACS PublicationsRequest reuse permissionsArticle Views870Altmetric-Citations120LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Heterogeneity of Mutations in the Acid β-Glucosidase Gene of Gaucher Disease Patients (1991)

Gaucher disease is inherited in an autosomal recessive manner and is the most prevalent lysosomal storage disease. Gaucher disease has marked phenotypic variation and molecular heterogeneity, and several simple and complex alleles of the acid beta-glucosidase gene have been identified as causal to this disease. Certain combinations of alleles have been shown to correlate well with the severity of the disease, but many Gaucher disease patients exist whose disease is not explained by any of the published mutations. This study was undertaken to identify mutant alleles in such incompletely characterized Gaucher disease, in an attempt to find further correlations between clinical phenotype and the presence of acid beta-glucosidase alleles. RNA was isolated from Gaucher cell lines and converted to cDNA, the cDNA was amplified by PCR and cloned, and several clones for each allele were sequenced. Several new singly mutated and multiply mutated alleles were identified, and sequence-specific oligonucleotide hybridization was used to verify the presence of these mutations in the genome of these patients. All newly identified mutations occurred only rarely in the Gaucher disease population, making it difficult to determine whether inheritance of a particular combination of alleles always correlates with the clinical manifestations seen in the test patients. Three of the newly described alleles were single missense mutations in exon 8, one was a single missense mutation in exon 5, and the fifth was a complex allele, comprising a series of different point mutations scattered throughout exons 5 and 6.(ABSTRACT TRUNCATED AT 250 WORDS)
OX133, a monoclonal antibody recognizing protein-bound N-ethylmaleimide for the identification of reduced disulfide bonds in proteins (2016)

In vivo, enzymatic reduction of some protein disulfide bonds, allosteric disulfide bonds, provides an important level of structural and functional regulation. The free cysteine residues generated can be labeled by maleimide reagents, including biotin derivatives, allowing the reduced protein to be detected or purified. During the screening of monoclonal antibodies for those specific for the reduced forms of proteins, we isolated OX133, a unique antibody that recognizes polypeptide resident, N-ethylmaleimide (NEM)-modified cysteine residues in a sequence-independent manner. OX133 offers an alternative to biotin-maleimide reagents for labeling reduced/alkylated antigens and capturing reduced/alkylated proteins with the advantage that NEM-modified proteins are more easily detected in mass spectrometry, and may be more easily recovered than is the case following capture with biotin based reagents.
Theoretical Study of Radar Polarization Parameters Obtained from Cirrus Clouds (1991)

A theoretical investigation of radar polarization parameters that characterize cloud ice backscattering is presented. The parameters considered were those commonly used in radar polarimetrics such as differential reflectivity (ZDR), linear depolarization ratio (LDR), circular depolarization ratio (CDR), intrinsic degree of orientation (ORTT) as well as conventional reflectivities. Experimental data on the shapes of ice crystals and their orientations are taken into account. Results suggest that prolate-shaped scatterers can be distinguished from those having oblate shapes by analyzing the depolarization ratio dependence on the elevation angle. Calculations suggest that circular polarization parameters provide stronger signals in a cross-polar channel and also show a 1esser dependence on scatterer orientation in comparison with linear polarization parameters. Propagation effects do not significantly affect the polarization parameters for equivalent water contents and cloud thicknesses that are typical for cirrus clouds. Differential phase shift that might be observed in cirrus clouds is relatively small. Finally, equivalent reflectivity factors are analyzed for several ice particle types as a function of their major axis. Reflectivity dependence on particle shapes is demonstrated, and comments on the possibility of making approximate estimates of cloud particle sizes are given.
Reliability and Validity of Selected PROMIS Measures in People with Rheumatoid Arthritis (2015)

These data provide preliminary evidence of reliability and construct validity of PROMIS CATs to assess RA symptoms and impacts, and feasibility of use in clinical care. PROMIS instruments captured the experiences of RA patients across the broad continuum of RA symptoms and function, especially at low disease activity levels. Future research is needed to evaluate performance in relevant subgroups, assess responsiveness and identify clinically meaningful changes.
A novel WD40-repeat protein involved in formation of epidermal bladder cells in the halophyte quinoa (2020)

Halophytes are plants that grow in high-salt environments and form characteristic epidermal bladder cells (EBCs) that are important for saline tolerance. To date, however, little has been revealed about the formation of these structures. To determine the genetic basis for their formation, we applied ethylmethanesulfonate mutagenesis and obtained two mutants with reduced levels of EBCs (rebc) and abnormal chloroplasts. In silico subtraction experiments revealed that the rebc phenotype was caused by mutation of REBC, which encodes a WD40 protein that localizes to the nucleus and chloroplasts. Phylogenetic and transformant analyses revealed that the REBC protein differs from TTG1, a WD40 protein involved in trichome formation. Furthermore, rebc mutants displayed damage to their shoot apices under abiotic stress, suggesting that EBCs may protect the shoot apex from such stress. These findings will help clarify the mechanisms underlying EBC formation and function.
T-lymphocytes from AIDS Patients Are Unable to Synthesize Ribonucleotides de Novo in Response to Mitogenic Stimulation (1995)

Proliferative defects have been reported at the level of DNA synthesis, even in T-lymphocytes from asymptomatic human immunodeficiency virus type-1+ (HIV-1+) patients. Since purine and pyrimidine ribonucleotide availability is crucial for proliferation, we compared the ability of HIV-1- and HIV-1+ T-lymphocytes (> 95% CD4+ and CD8+) to activate de novo biosynthetic and salvage pathways following phytohemagglutinin stimulation using 14C-labeled precursors. The striking abnormality already detectable in asymptomatic patients' cells was the impaired ability of CTP, UDP-Glc, and UTP pools to expand over 72 h (44-70% of control), although ATP and GTP pools and responses were normal. In symptomatic patients, resting T-cells showed markedly reduced pyrimidine pools (53-74% of control) with no change following activation. Relatively normal ATP, GTP, and NAD pools masked the same impaired response of de novo synthesis to activation, with ATP and GTP being reduced by 50% at 48 h. Purine salvage was more active than the control in unstimulated HIV-1+ cells. This impaired de novo synthesis in HIV-1+ T-lymphocytes severely restricts the availability of ribonucleotides for vital growth-related activities such as membrane expansion and strand break repair as well as DNA and RNA synthesis. The data indicate that resting T-lymphocytes from symptomatic patients survive through enhanced salvage, but the stimulation induces metabolic cell death, and provide an explanation for the activation-associated lymphocyte death seen in HIV-1+ T-lymphocytes.
Synthesis and stereochemistry of some 8-substituted 2-methyldecahydroisoquinolines (1974)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis and stereochemistry of some 8-substituted 2-methyldecahydroisoquinolinesIan W. Mathison and Phillip H. MorganCite this: J. Org. Chem. 1974, 39, 22, 3210–3215Publication Date (Print):November 1, 1974Publication History Published online1 May 2002Published inissue 1 November 1974https://pubs.acs.org/doi/10.1021/jo00936a006https://doi.org/10.1021/jo00936a006research-articleACS PublicationsRequest reuse permissionsArticle Views175Altmetric-Citations18LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
교통사고비용과 EPDO에 근거한 사고밀도 청주사례 분석 (2018)

This study deals with traffic accident models based on 30 zones of Cheongju. The goal of this study is to develop the accident density models reflecting the land use, transportation and socioeconomic characteristics. The models are developed through multiple linear regression analyses. The dependent variables are ‘traffic accident costs’ and ‘EPDO’. The independent variables are land-use, traffic and socioeconomic variables. The results are the followings. First, in the accident costs density model which is tested to be statistically significant, commercial area ratio, major arterial road length, woman ratio and number of companies are analyzed to be positive to the models. Second, in the EPDO density model, commercial area ratio and major arterial road length are analyzed to be positive, and bikeway density and the number of transportation industry employees to be negative to the model.
Picosecond ultrasonics study of the vibrational modes of a nanostructure (2002)

We report experiments in which a subpicosecond pump light pulse is used to excite vibrations in a nanostructure consisting of a periodic array of copper wires embedded in a glass matrix on a silicon substrate. The motion of the wires after excitation is detected using a time-delayed probe light pulse. From the measured data, it is possible to determine the frequencies νn and damping rates Γn of a number of the normal modes of the structure. These modes have frequencies lying in the range 1–30 GHz. By comparison of the measured νn and Γn with the frequencies and damping rates calculated from a computer simulation of the vibrations of the nanostructure, we have been able to deduce the vibration patterns of six of the normal modes.