gebhart/patrepeval huggingface dataset:
Resonant cavity imaging biosensor (2005)

The resonant cavity imaging biosensor (RCIB) detects binding between target biomolecules in solution and probe biomolecules fixed to a microarray surface. IR light couples resonantly through a 1 cm square cavity constructed from Si/SiO/sub 2/ Bragg mirrors that contains the microarray surface. As the wavelength of the probe beam is swept, an IR camera monitors cavity transmittance at each pixel. The current setup detects 5 nm relief structures etched in the SiO/sub 2/ surface of one of the Bragg mirrors. RCIB improves on existing label-free biosensing techniques by offering the combination of high sensitivity, high throughput, and real-time observation.
Analysis of Wandering Spur Patterns in a Fractional-$N$ Frequency Synthesizer With a MASH-Based Divider Controller (2020)

The Multi-stAge noise SHaping Digital Delta-Sigma Modulator (MASH DDSM), which is commonly used as a divider controller in a fractional-N frequency synthesizer, can produce a time-varying periodic pattern of tones in the output spectrum of the synthesizer; these are known as wandering spurs. In this work, a detailed analysis of the cause of wandering spur patterns in a MASH 1-1-1 DDSM-based fractional-N frequency synthesizer, supported by experimental measurements, is presented.
Next-Generation Bacillus anthracis Live Attenuated Spore Vaccine Based on the htrA- (High Temperature Requirement A) Sterne Strain (2016)

Anthrax is a lethal disease caused by the gram-positive spore-producing bacterium Bacillus anthracis. Live attenuated vaccines, such as the nonencapsulated Sterne strain, do not meet the safety standards mandated for human use in the Western world and are approved for veterinary purposes only. Here we demonstrate that disrupting the htrA gene, encoding the chaperone/protease HtrA (High Temperature Requirement A), in the virulent Bacillus anthracis Vollum strain results in significant virulence attenuation in guinea pigs, rabbits and mice, underlying the universality of the attenuated phenotype associated with htrA knockout. Accordingly, htrA disruption was implemented for the development of a Sterne-derived safe live vaccine compatible with human use. The novel B. anthracis SterneΔhtrA strain secretes functional anthrax toxins but is 10-10(4)-fold less virulent than the Sterne vaccine strain depending on animal model (mice, guinea pigs, or rabbits). In spite of this attenuation, double or even single immunization with SterneΔhtrA spores elicits immune responses which target toxaemia and bacteremia resulting in protection from subcutaneous or respiratory lethal challenge with a virulent strain in guinea pigs and rabbits. The efficacy of the immune-protective response in guinea pigs was maintained for at least 50 weeks after a single immunization.
On the Design and Control of Exoskeleton Knee (2013)

This paper describes a lightweight (2.7 pounds) exoskeleton orthotics knee which provides controllable resisting torque. In particular, exoskeleton knee uses friction forces between two surfaces to provide resistive torque and impede knee flexion. Creating an impeding torque at the exoskeleton knee will decrease the torque that needs to be provided by the wearer at his/her knee during flexion. The required external power (from batteries) to provide the controllable resistive torque is minimal in comparison to the dissipated locomotion power since the resistive torque generation is “self-energizing” and is using the energy of the knee itself for braking. The exoskeleton knee uses the absolute angle of the thigh for basic functionality; no other measurements such as ground reaction force or the knee joint angle are necessary for basic performance. This allows the exoskeleton knee to be worn not only independently on the wearer’s knee but also in conjunction with hip, ankle or foot exoskeletons. This gives a great deal of flexibility for use of exoskeleton knees in variety of medical, civilian and military applications.
Growth Dynamics During Dropwise Condensation on Nanostructured Superhydrophobic Surfaces (2012)

Condensation on superhydrophobic nanostructured surfaces offers new opportunities for enhanced energy conversion, efficient water harvesting, and high performance thermal management. Such surfaces are designed to be Cassie stable, which minimize contact line pinning and allow for passive shedding of condensed water droplets at sizes smaller than the capillary length. In this work, we investigated in situ water condensation on superhydrophobic nanostructured surfaces using environmental scanning electron microscopy (ESEM). The “Cassie stable” surfaces consisted of silane coated silicon nanopillars with diameters of 300 nm, heights of 6.1 μm, and spacings of 2 μm, but allowed droplets of distinct suspended (S) and partially wetting (PW) morphologies to coexist. With these experiments combined with thermal modeling of droplet behavior, the importance of initial growth rates and droplet morphology on heat transfer is elucidated. The effect of wetting morphology on heat transfer enhancement is highlighted with observed 6× higher initial growth rate of PW droplets compared to S droplets. Consequently, the heat transfer of the PW droplet is 4–6× higher than that of the S droplet. To compare the heat transfer enhancement, PW and S droplet heat transfer rates are compared to that of a flat superhydrophobic silane coated surface, showing a 56% enhancement for the PW morphology, and 71% degradation for the S morphology. This study provides insight into importance of local wetting morphology on droplet growth rate during superhydrophobic condensation, as well as the importance of designing CB stable surfaces with PW droplet morphologies to achieve enhanced heat transfer during dropwise condensation.
tRNA dynamics on the ribosome during translation (2004)

Using single-molecule fluorescence spectroscopy, time-resolved conformational changes between fluorescently labeled tRNA have been characterized within surface-immobilized ribosomes proceeding through a complete cycle of translation elongation. Fluorescence resonance energy transfer was used to observe aminoacyl-tRNA (aa-tRNA) stably accommodating into the aminoacyl site (A site) of the ribosome via a multistep, elongation factor-Tu dependent process. Subsequently, tRNA molecules, bound at the peptidyl site and A site, fluctuate between two configurations assigned as classical and hybrid states. The lifetime of classical and hybrid states, measured for complexes carrying aa-tRNA and peptidyl-tRNA at the A site, shows that peptide bond formation decreases the lifetime of the classical-state tRNA configuration by approximately 6-fold. These data suggest that the growing peptide chain plays a role in modulating fluctuations between hybrid and classical states. Single-molecule fluorescence resonance energy transfer was also used to observe aa-tRNA accommodation coupled with elongation factor G-mediated translocation. Dynamic rearrangements in tRNA configuration are also observed subsequent to the translocation reaction. This work underscores the importance of dynamics in ribosome function and demonstrates single-particle enzymology in a system of more than two components.
HitFraud: A Broad Learning Approach for Collective Fraud Detection in Heterogeneous Information Networks (2017)

On electronic game platforms, different payment transactions have different levels of risk. Risk is generally higher for digital goods in e-commerce. However, it differs based on product and its popularity, the offer type (packaged game, virtual currency to a game or subscription service), storefront and geography. Existing fraud policies and models make decisions independently for each transaction based on transaction attributes, payment velocities, user characteristics, and other relevant information. However, suspicious transactions may still evade detection and hence we propose a broad learning approach leveraging a graph based perspective to uncover relationships among suspicious transactions, i.e., inter-transaction dependency. Our focus is to detect suspicious transactions by capturing common fraudulent behaviors that would not be considered suspicious when being considered in isolation. In this paper, we present HitFraud that leverages heterogeneous information networks for collective fraud detection by exploring correlated and fast evolving fraudulent behaviors. First, a heterogeneous information network is designed to link entities of interest in the transaction database via different semantics. Then, graph based features are efficiently discovered from the network exploiting the concept of meta-paths, and decisions on frauds are made collectively on test instances. Experiments on real-world payment transaction data from Electronic Arts demonstrate that the prediction performance is effectively boosted by HitFraud with fast convergence where the computation of meta-path based features is largely optimized. Notably, recall can be improved up to 7.93% and F-score 4.62% compared to baselines.
A novel process for manufacture of methanol. Progress report, December 1, 1989--February 28, 1990 (1990)

A bench-scale reactor is being used to conduct studies of the conversion of synthesis gas to methanol by a novel process. During the last quarter, the effect of potassium methoxide and Cu-chromite loading on the MeOH formation rate was investigated. The rate obtained with Cu-chromite was compared to that using Cu-ZnO as catalyst. Work also continued on the modification of the experimental equipment to permit on-line monitoring of liquid and gas compositions.
Embedded-stripe GaAs-GaAlAs double-heterostructure lasers with polycrystalline GaAsP layers - I: Lasers with cleaved mirrors (1977)

We report a new type of embedded stripe laser in which low-order mode CW oscillation is successfully attained at room temperature. In order to achieve lateral current confinement, a high-resistivity polycrystalline GaAsP layer is formed around the mesa stripe by selective vapor-phase growth technology. It turns out that a stable single-mode operation is attained at an injected current up to several times the threshold value.
Interval Extensions of Signed Distance Functions: Uniform Samplings and the Range of Associated Implicit Objects (2008)

This paper considers the problem of inferring the geometry of an object from values of the signed distance sampled on a uniform grid. The problem is motivated by the desire to effectively and efficiently model objects obtained by 3D imaging technology that is now ubiquitous in medical diagnostics. Recently developed techniques for automated segmentation convert intensity to signed distance, and the voxel structure imposes the uniform sampling grid. While specification of the signed distance function throughout the ambient space would provide an implicit model that uniquely specifies the object, a set of uniformly sampled signed distance values may uniquely determine neither the distance function nor the shape of the object. Here we employ essential properties of the signed distance to construct upper and lower bounds on the allowed variation in signed distance in 1, 2, and 3 dimensions. The bounds are combined to produce interval-valued extensions of the signed distance function including a tight global extension and more computationally efficient local bounds that provide useful criteria for root exclusion/isolation.
Identification of Novel Survivin-Derived CTL Epitopes with Different HLA-A-Restriction Profiles (2004)

The identification of tumor antigens, which are essential for the survival of tumor cells is a new avenue to prevent antigen loss variants emerging due to immunoselection, particularly during immune therapy. In the search for such immunogenic tumor antigens, we recently identified spontaneous cytotoxic lymphocyte (CTL) responses against the inhibitor of apoptosis protein survivin. Thus, we identified two HLA-A2-restricted, survivin-derived CTL epitopes, which both were targets for spontaneous CTL responses in melanoma, breast cancer, and CLL. Here, we extend these data and describe the characterization of novel HLA-A1-, HLA-A2-, HLA-A3-, and HLA-A11-restricted survivin epitopes on the basis of spontaneous CTL responses in cancer patients. These epitopes significantly increase the number of patients eligible for immunotherapy based on survivin derived peptides. Additionally, the collective targeting of several restriction elements is likely to decrease the risk of immune escape by HLA-allele loss.
61.2: Phosphorescent Stacked OLEDs for Warm White Lighting Applications (2013)

Abstract We report record lifetime for an all‐phosphorescent stacked white OLED pixel with an extended lifetime to LT70=25,000 hours at L 0 =3,000 cd/m 2 with 1.72x external outcoupling enhancement and a power efficacy of 60 lm/W. This warm white device meets En ergy Star criteria with CIE 1931 (x, y)=(0.452, 0.420), CCT=2,882K, CRI=83, and demonstrates exceptional color stability as a function of luminance and aging.
Advancing Connectionist Temporal Classification With Attention Modeling (2018)

In this study, we propose advancing all-neural speech recognition by directly incorporating attention modeling within the Connectionist Temporal Classification (CTC) framework. In particular, we derive new context vectors using time convolution features to model attention as part of the CTC network. To further improve attention modeling, we utilize content information extracted from a network representing an implicit language model. Finally, we introduce vector based attention weights that are applied on context vectors across both time and their individual components. We evaluate our system on a 3400 hours Microsoft Cortana voice assistant task and demonstrate that our proposed model consistently outperforms the baseline model achieving about 20% relative reduction in word error rates.
Conversion of Visible Light to Electrical Energy: Stable Cadmium Selenide Photoelectrodes in Aqueous Electrolytes (1977)

Stabilization of n-type CdSe to photoanodic dissolution is reported. The stabilization is accomplished by the competitive oxidation of S2- or Sn2- at the CdSe photoanode in an electrochemical cell. Such stabilized cells are shown to sustain the conversion of low energy (≥ 1.7 eV) visible light to electricity with good efficiency and no deterioration of the CdSe photoelectrode or of the electrolyte. The electrolyte undergoes no net chemical change because the oxidation occurring at the photoelectrode is reversed at the cathode. Conversion of monochromatic light at 633 nm to electricity is shown to be up to ~9% efficient with output potentials of ~ 0.4 V. Conversion of solar energy to electricity is estimated to be ~ 2% efficient.
High isolation slot coupled antenna with integrated tunable self‐interference cancellation circuitry (2018)

This letter presents a high interport isolation, compact dual polarised slot coupled monostatic patch antenna with integrated tunable analogue/RF self‐interference cancellation (SIC) circuitry for 2.4/2.5 GHz In Band Full Duplex wireless applications. The presented antenna deploys hybrid feeding for improved interport isolation through polarisation diversity and integrated single‐tap RF SIC circuitry provides additional isolation on top of antenna isolation. Brief mathematical description for deployed single‐tap RF SIC is also presented. The implemented prototype of the proposed antenna module provides around 80 dB interport isolation for 20 MHz bandwidth and better than 97 dB peak isolation when measured in a lab in the presence of environmental reflections. Moreover, 20 MHz SIC bandwidth with 80 dB interport isolation can be tuned within antenna's 10 dB return loss impedance bandwidth of 60 MHz.
Nanobody-Based Enzyme Immunoassay for Aflatoxin in Agro-Products with High Tolerance to Cosolvent Methanol (2014)

A phage-displayed library of variable domain of heavy chain of the heavy chain antibody (VHH) or nanobody (Nb) was constructed after immunizing an alpaca with aflatoxin B1 (AFB1) conjugated with bovine serum albumin (AFB1-BSA). Two AFB1-specific nanobodies were selected. The obtained nanobodies were compared to an aflatoxin-specific monoclonal antibody B5 with respect to stability under organic solvents and high temperature. The two nanobodies could bind antigen specifically after exposure to temperatures as high as 95 °C. Besides, the nanobodies showed better or similar tolerance to organic solvents. A competitive ELISA with nanobody Nb26 was developed for the analysis of AFB1, exhibiting an IC50 value of 0.754 ng/mL (2.4 μM), linear range from 0.117 to 5.676 ng/mL. Due to the high tolerance to methanol, sample extracts were analyzed by nanobody-based ELISA without dilution. The recovery from spiked peanut, rice, corn and feedstuff ranged from 80 to 115%. In conclusion, the isolated nanobodies are excellent candidates for immunoassay application in aflatoxin determination.
Partial correction of defective Cl<sup>−</sup>secretion in cystic fibrosis epithelial cells by an analog of squalamine (2001)

Defective cystic fibrosis (CF) transmembrane conductance regulator (CFTR)-mediated Cl(-) transport across the apical membrane of airway epithelial cells is implicated in the pathophysiology of CF lungs. A strategy to compensate for this loss is to augment Cl(-) transport through alternative pathways. We report here that partial correction of this defect could be attained through the incorporation of artificial anion channels into the CF cells. Introduction of GL-172, a synthetic analog of squalamine, into CFT1 cells increased cell membrane halide permeability. Furthermore, when a Cl(-) gradient was generated across polarized monolayers of primary human airway or Fischer rat thyroid cells in an Ussing chamber, addition of GL-172 caused an increase in the equivalent short-circuit current. The magnitude of this change in short-circuit current was ~30% of that attained when CFTR was maximally stimulated with cAMP agonists. Patch-clamp studies showed that addition of GL-172 to CFT1 cells also increased whole cell Cl(-) currents. These currents displayed a linear current-voltage relationship and no time dependence. Additionally, administration of GL-172 to the nasal epithelium of transgenic CF mice induced a hyperpolarization response to perfusion with a low-Cl(-) solution, indicating restoration of Cl(-) secretion. Together, these results demonstrate that in CF airway epithelial cells, administration of GL-172 is capable of partially correcting the defective Cl(-) secretion.
Conformationally Defined Rexinoids and Their Efficacy in the Prevention of Mammary Cancers (2015)

(2E,4E,6Z,8Z)-8-(3',4'-Dihydro-1'(2H)-naphthalen-1'-ylidene)-3,7-dimethyl-2,3,6-octatrienoinic acid (UAB30) is currently undergoing clinical evaluation as a novel cancer prevention agent. In efforts to develop even more highly potent rexinoids that prevent breast cancer without toxicity, we further explore here the structure-activity relationship of two separate classes of rexinoids. UAB30 belongs to the class II rexinoids and possesses a 9Z-tetraenoic acid chain bonded to a tetralone ring, whereas the class I rexinoids contain the same 9Z-tetraenoic acid chain bonded to a disubstituted cyclohexenyl ring. Among the 12 class I and class II rexinoids evaluated, the class I rexinoid 11 is most effective in preventing breast cancers in an in vivo rat model alone or in combination with tamoxifen. Rexinoid 11 also reduces the size of established tumors and exhibits a therapeutic effect. However, 11 induces hypertriglyceridemia at its effective dose. On the other hand rexinoid 10 does not increase triglyceride levels while being effective in the in vivo chemoprevention assay. X-ray studies of four rexinoids bound to the ligand binding domain of the retinoid X receptor reveal key structural aspects that enhance potency as well as those that enhance the synthesis of lipids.
Pentafluorosulfanylnitramide Salts (2000)

The synthesis and properties of a new class of inorganic salts, named pentafluorosulfanylnitramide salts (or pentafluorosulfanylnitraminic acid salts) [Z+SF5NNO2-], are described. A number of SF5-nitramide salts (Z+SF5NNO2-) were successfully prepared via nucleophilic displacements from carbamates and/or ion exchange techniques, but some salts [M(SF5NNO2)x; M = Li, Mg, Al] decomposed during isolation procedures and appear to be unstable in the solid state. Single-crystal X-ray diffraction was used to fully characterize the Z+SF5NNO2-, and their properties/structures are compared with those of the corresponding dinitramide salts (or dinitraminic acid salts), Z+N(NO2)2-. X-ray crystallography revealed major structural differences between N(NO2)2- and SF5N(NO2)- salts concerning the N-N distances and the angles subtended at the central nitrogen atom. In the N(NO2)2- salts, there are two nonequivalent N-N (average lengths 1.372(2) and 1.354(2) A) distances and an average N-N-N angle of 115.8(3) degrees (falls between sp3 and sp2 hybridization). In the SFsNNO2- salts, the average N-N distance is much shorter, 1.308(9) A, and the average N-N-S angle is 120.0(5) degrees (closely fits sp2 hybridization). The SF5NNO2- salts show a remarkable metrical similarity for the SF5 moiety in all structures, indicating a lack of sensitivity to its steric and electronic environment. This is in marked contrast to N(NO2)2-, where there is a wide variation in conformations adopted by these anions which can be related to their environment.
<i>MKS3/TMEM67</i>mutations are a major cause of COACH Syndrome, a Joubert Syndrome related disorder with liver involvement (2008)

The acronym COACH defines an autosomal recessive condition of Cerebellar vermis hypo/aplasia, Oligophrenia, congenital Ataxia, Coloboma and Hepatic fibrosis. Patients present the "molar tooth sign", a midbrain-hindbrain malformation pathognomonic for Joubert Syndrome (JS) and Related Disorders (JSRDs). The main feature of COACH is congenital hepatic fibrosis (CHF), resulting from malformation of the embryonic ductal plate. CHF is invariably found also in Meckel syndrome (MS), a lethal ciliopathy already found to be allelic with JSRDs at the CEP290 and RPGRIP1L genes. Recently, mutations in the MKS3 gene (approved symbol TMEM67), causative of about 7% MS cases, have been detected in few Meckel-like and pure JS patients. Analysis of MKS3 in 14 COACH families identified mutations in 8 (57%). Features such as colobomas and nephronophthisis were found only in a subset of mutated cases. These data confirm COACH as a distinct JSRD subgroup with core features of JS plus CHF, which major gene is MKS3, and further strengthen gene-phenotype correlates in JSRDs.
Identification, purification, and characterization of a protein activator (PA28) of the 20 S proteasome (macropain). (1992)

A protein that greatly stimulates the multiple peptidase activities of the 20 S proteasome (also known as macropain, the multicatalytic protease complex, and 20 S protease) has been purified from bovine red blood cells and from bovine heart. The activator protein was a single polypeptide with an apparent molecular weight of 28,000, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and had a native molecular weight of approximately 180,000. This protein, which we have termed PA28, regulated all three of the putatively distinct peptidase activities displayed by each of two functionally different forms of the proteasome. This regulation usually included both an increase in the maximal reaction velocity and a decrease in the concentration of substrate required for half-maximal velocity and indicated that PA28 acted as a positive allosteric effector of the proteasome. PA28 failed, however, to stimulate the hydrolysis of large protein substrates such as casein and lysozyme. These results suggested that the hydrolysis of protein substrates occurred at a site or sites distinct from those that hydrolyzed small peptides and that the regulation of the two processes could be uncoupled. Evidence for direct binding of PA28 to the proteasome was obtained by glycerol density gradient centrifugation. PA28 may play an important regulatory role in intracellular proteolytic pathways mediated by the proteasome.