gebhart/patrepeval huggingface dataset:
Fatty acid synthesis in mitochondria from <i>Saccharomyces cerevisiae</i> (1987)

The ability of purified mitochondria isolated from S. cerevisiae to synthesize fatty acids and especially very long chain fatty acids (VLCFA) has been investigated. The VLCFA synthesis requires malonyl-CoA as the C2 unit donor and NADPH as the reducing agent. Moreover the yeast mitochondrial elongase is able to accept either exogenous long chain fatty acyl-CoAs as substrates or elongate endogenous substrates. In the latter case, ATP is required for full activity. Besides this important VLCFA formation, the mitochondria from S. cerevisiae were also able to synthesize C16 and C18.
Changes in Red Cell Deformability Associated with Anaesthesia and Cardiopulmonary Bypass in Open-Heart Surgery (1985)

Red cell deformability was observed during open-heart surgery in 59 patients. Deformability, assessed with a standard microfiltration method, was expressed as red cell filtration rate (RFR) in microliter/s. The mean preoperative value, 38.9 +/- 1.0 microliter/s, showed a generally falling tendency. The first significant decrease in mean RFR (by 18%) followed induction of general anaesthesia, and the second (by 15%) was seen 60 min after the start of cardiopulmonary bypass (CPB). The major decrease in RFR during CPB was found at the end of bypass, when it was reduced to 62% of the pre-CPB value. The percentage RFR reduction at the end of CPB showed significant correlation with 1) CPB duration (r = 0.49), 2) oxygen flow rate index (OFRI), i.e. flow/min in the bubble oxygenator/m2 bsa (r = 0.38), and 3) blood flow rate index (BFRI), i.e. average volume of blood pumped through the heart-lung machine/min CPB time/m2 bsa (r = 0.51).
Wearable Systems for Monitoring Mobility-Related Activities in Chronic Disease: A Systematic Review (2010)

The use of wearable motion sensing technology offers important advantages over conventional methods for obtaining measures of physical activity and/or physical functioning in individuals with chronic diseases. This review aims to identify the actual state of applying wearable systems for monitoring mobility-related activity in individuals with chronic disease conditions. In this review we focus on technologies and applications, feasibility and adherence aspects, and clinical relevance of wearable motion sensing technology. PubMed (Medline since 1990), PEdro, and reference lists of all relevant articles were searched. Two authors independently reviewed randomised trials systematically. The quality of selected articles was scored and study results were summarised and discussed. 163 abstracts were considered. After application of inclusion criteria and full text reading, 25 articles were taken into account in a full text review. Twelve of these papers evaluated walking with pedometers, seven used uniaxial accelerometers to assess physical activity, six used multiaxial accelerometers, and two papers used a combination approach of a pedometer and a multiaxial accelerometer for obtaining overall activity and energy expenditure measures. Seven studies mentioned feasibility and/or adherence aspects. The number of studies that use movement sensors for monitoring of activity patterns in chronic disease (postural transitions, time spent in certain positions or activities) is nonexistent on the RCT level of study design. Although feasible methods for monitoring human mobility are available, evidence-based clinical applications of these methods in individuals with chronic diseases are in need of further development.
Self-limiting Growth Behavior of Epitaxial NiSi<sub>2</sub> and its Impact on Controlled Silicidation of Metal Source/Drain in Silicon Nanowire MOSFETs (2008)

2008 International Conference on Solid State Devices and Materials,Self-limiting Growth Behavior of Epitaxial NiSi<sub>2</sub> and its Impact on Controlled Silicidation of Metal Source/Drain in Silicon Nanowire MOSFETs
Mechanochemical Diels-Alder Cycloaddition Reactions for Straightforward Synthesis of endo-Norbornene Derivatives (2010)

Under mechanochemical milling conditions, Diels-­Alder cycloaddition of cyclopentadiene with maleic anhydride and maleimide derivatives proceeded very smoothly, affording endo-norbornenes exclusively in quantitative yield. All the transformations were accomplished at room temperature without using any catalyst or organic solvent, thus the workup and purification procedure is very simple. Control experiments on traditional and other tentative conditions were also investigated.
Irradiance Gradients in the Presence of Participating Media and Occlusions (2008)

Abstract In this paper we present a technique for computing translational gradients of indirect surface reflectance in scenes containing participating media and significant occlusions. These gradients describe how the incident radiance field changes with respect to translation on surfaces. Previous techniques for computing gradients ignore the effects of volume scattering and attenuation and assume that radiance is constant along rays connecting surfaces. We present a novel gradient formulation that correctly captures the influence of participating media. Our formulation accurately accounts for changes of occlusion, including the effect of surfaces occluding scattering media. We show how the proposed gradients can be used within an irradiance caching framework to more accurately handle scenes with participating media, providing significant improvements in interpolation quality.
<i>Escherichia coli</i> Heat‐Labile Enterotoxin Binds to Glycosylated Proteins with Lactose by Amino Carbonyl Reaction (1994)

The binding of Escherichia coli heat-labile enterotoxin (LT) type I to glycosylated proteins with lactose (Gal beta 1-4Glc) by amino carbonyl reaction was studied by the Western blot assay and by the microtiter well binding assay. LT bound to a lactose-alpha-lactalbumin amino carbonyl product (Lac-LA), whereas cholera toxin did not. The binding ability of Lac-LA was abolished by beta-galactosidase treatment, indicating that the terminal galactose is essential for the binding of LT. The binding of LT to Lac-LA was inhibited by galactose and lactose, and most effectively inhibited by lactulose (Gal beta 1-4Fru), which is a structural analog of the Amadori rearrangement product of the amino carbonyl reaction between lactose and an epsilon-amino group of a lysine residue (lactuloselysine). The results suggest that LT recognizes the portion of lactuloselysine in Lac-LA. LT also bound to a melibiose (Gal alpha 1-6Glc)-alpha-lactalbumin amino carbonyl product (Mel-LA), but the binding ability of Mel-LA was weaker than that of Lac-LA, suggesting that the beta 1-4 linked terminal galactose is dispensable but preferable for the binding. Furthermore, LT bound to the amino carbonyl products of lactose with beta-lactoglobulin, caseins, bovine serum albumin, and ovalbumin. These results indicate that LT binds to the amino carbonyl products between proteins and sugars containing the terminal galactose, such as lactose.
Multi-layer Tablet: Current scenario and recent advances (2013)

The purpose of this article on tableting of multiple Active Pharmaceutical Ingredients (APIs) in the single oral solid dose in the form of Fixed Dose Combinations (FDCs), that focused on therapeutic justification, designing, its practical approach, summarize the problem encountered and parameters to be considered during development. This will help budding formulation development scientists working on generic development as such types of formulation is current need in various disease conditions. Looking to the necessity of medical practitioners, ultimately generic pharmaceutical companies taking interest in development of such formulation with correct medical justification due to various health problem not only in India but also globally. FDCs are highly popular in the Indian pharmaceutical market and also have been seen in the last few years. Presently very few generic companies are working on FDCs in the form of multilayer tablets resulting into small numbers of formulation in the market yearly.
A comparative study of two Boolean formulations of FPGA detailed routing constraints (2004)

We present empirical analyses of two Boolean satisfiability (SAT) formulations of FPGA (field programmable gate array) detailed routing constraints. Boolean SAT-based routing transforms a routing problem into a Boolean SAT instance by rendering geometric routing constraints as an atomic Boolean function. The generated Boolean function is satisfiable if and only if the corresponding routing is possible. Two different Boolean SAT-based routing models are analyzed: the track-based and the route-based routing constraint model. The track-based routing model transforms a routing task into a net-to-track assignment problem, whereas the route-based routing model reduces it into a routability-checking problem with explicitly enumerated set of detailed routes for nets. In both models, routing constraints are represented as CNF Boolean satisfiability clauses. Through comparative experiments, we demonstrate that the route-based formulation yields an easier-to-evaluate and more scalable routability Boolean function than the track-based method. This is empirical evidence that a smart/efficient Boolean formulation can achieve significant performance improvement in real-world applications.
Gain-of-function Na <sub>v</sub> 1.8 mutations in painful neuropathy (2012)

Painful peripheral neuropathy often occurs without apparent underlying cause. Gain-of-function variants of sodium channel Na(v)1.7 have recently been found in ∼30% of cases of idiopathic painful small-fiber neuropathy. Here, we describe mutations in Na(v)1.8, another sodium channel that is specifically expressed in dorsal root ganglion (DRG) neurons and peripheral nerve axons, in patients with painful neuropathy. Seven Na(v)1.8 mutations were identified in 9 subjects within a series of 104 patients with painful predominantly small-fiber neuropathy. Three mutations met criteria for potential pathogenicity based on predictive algorithms and were assessed by voltage and current clamp. Functional profiling showed that two of these three Na(v)1.8 mutations enhance the channel's response to depolarization and produce hyperexcitability in DRG neurons. These observations suggest that mutations of Na(v)1.8 contribute to painful peripheral neuropathy.
Utilizing Round Robin Concept for Load Balancing Algorithm at Virtual Machine Level in Cloud Environment (2014)

Day by Day increasing traffic on internet introduces the requirement of load balancing concept to get the most utilization of the resources available on Cloud. There are so many complex calculations and concepts implemented to achieve better and better resource utilization and performance. Out of all this complexity the Rounrobin algorithm provide the simplest solution for load balancing in cloud environment. So we are going to use roun robin as a base algorithm and propose an improved version of load balancing algorithm in the paper.
A Defect in Menadione Biosynthesis Induces Global Changes in Gene Expression in <i>Staphylococcus aureus</i> (2008)

Both the high-resolution two-dimensional protein gel electrophoresis technique and full-genome DNA microarrays were used for identification of Staphylococcus aureus genes whose expression was changed by a mutation in menD. Because the electron transport chain is interrupted, the mutant should be unable to use oxygen and nitrate as terminal electron acceptors. Consistent with this, a mutation in menD was found to cause a gene expression pattern typically detected under anaerobic conditions in wild-type cells: proteins involved in glycolytic as well as in fermentation pathways were upregulated, whereas tricarboxylic acid (TCA) cycle enzymes were significantly downregulated. Moreover, the expression of genes encoding enzymes for nitrate respiration and the arginine deiminase pathway was strongly increased in the mutant strain. These results indicate that the menD mutant, just as the site-directed S. aureus hemB mutant, generates ATP from glucose or fructose mainly by substrate phosphorylation and might be defective in utilizing a variety of carbon sources, including TCA cycle intermediates and compounds that generate ATP only via electron transport phosphorylation. Of particular interest is that there are also differences in the gene expression patterns between hemB and menD mutants. While some anaerobically active enzymes were present in equal amounts in both strains (Ldh1, SACOL2535), other classically anaerobic enzymes seem to be present in higher amounts either in the hemB mutant (e.g., PflB, Ald1, IlvA1) or in the menD mutant (arc operon). Only genes involved in nitrate respiration and the ald1 operon seem to be additionally regulated by a depletion of oxygen in the hemB and/or menD mutant.
Comparison of in situ Corneoscleral Disc Excision versus Whole Globe Enucleation in Cornea Donors Regarding Microbial Contamination in Organ Culture Medium a Prospective Monocentric Study over 9 Years (2012)

The rate of microbial contamination in organ-cultured donor corneas does not seem to be dependent on the method of their retrieval.
Thrombopoietin, flt3‐ligand and c‐kit‐ligand modulate <i>HOX</i> gene expression in expanding cord blood CD133<sup>+</sup> cells (2004)

Haemopoietic stem/progenitor cell (HSPC) development is regulated by extrinsic and intrinsic stimuli. Extrinsic modulators include growth factors and cell adhesion molecules, whereas intrinsic regulation is achieved with many transcription factor families, of which the HOX gene products are known to be important in haemopoiesis. Umbilical cord blood CD133+ HSPC proliferation potential was tested in liquid culture with 'TPOFLK' (thrombopoietin, flt-3 ligand and c-kit ligand, promoting HSPC survival and self-renewal), in comparison to 'K36EG' (c-kit-ligand, interleukins-3 and -6, erythropoietin and granulocyte colony-stimulating factor, inducing haemopoietic differentiation). TPOFLK induced a higher CD133+ HSPC proliferation (up to 60-fold more, at week 8) and maintained a higher frequency of the primitive colony-forming cells than K36EG. Quantitative polymerase chain reaction analysis revealed opposite expression patterns for specific HOX genes in expanding cord blood CD133+ HSPC. After 8 weeks in liquid culture, TPOFLK increased the expression of HOX B3, B4 and A9 (associated with uncommitted HSPC) and reduced the expression of HOX B8 and A10 (expressed in committed myeloid cells) when compared to K36EG. These results suggest that TPOFLK induces CD133+ HSPC proliferation, self-renewal and maintenance, up-regulation of HOX B3, B4 and A9 and down-regulation of HOX B8 and A10 gene expression.
Vision-Based Pose Estimation for Robot-Mediated Hand Telerehabilitation (2016)

Vision-based Pose Estimation (VPE) represents a non-invasive solution to allow a smooth and natural interaction between a human user and a robotic system, without requiring complex calibration procedures. Moreover, VPE interfaces are gaining momentum as they are highly intuitive, such that they can be used from untrained personnel (e.g., a generic caregiver) even in delicate tasks as rehabilitation exercises. In this paper, we present a novel master-slave setup for hand telerehabilitation with an intuitive and simple interface for remote control of a wearable hand exoskeleton, named HX. While performing rehabilitative exercises, the master unit evaluates the 3D position of a human operator's hand joints in real-time using only a RGB-D camera, and commands remotely the slave exoskeleton. Within the slave unit, the exoskeleton replicates hand movements and an external grip sensor records interaction forces, that are fed back to the operator-therapist, allowing a direct real-time assessment of the rehabilitative task. Experimental data collected with an operator and six volunteers are provided to show the feasibility of the proposed system and its performances. The results demonstrate that, leveraging on our system, the operator was able to directly control volunteers' hands movements.
Magnetostrictive Torque Sensors — Derivation of Transducer Model (1989)

<div class="htmlview paragraph">Current worldwide development activity and potential markets for magnetostrictive, noncontact, torque sensors are reviewed first. Then, a general model for the sensor is derived, and equivalent electrical and magnetic circuits for the sensor are developed. The model is defined in terms of a basic single-branch magnetostrictive sensor element, combinations of which serve as models for multi-branch sensors.</div> <div class="htmlview paragraph">Key assumptions are made that permit simplification of model equations. Procedures for reliable evaluation of magnetic permeance parameters (essential for insuring accuracy of the model) are also given.</div> <div class="htmlview paragraph">Physical origins of the sensor output signal are described, and discussion of characteristic behavior of magnetostrictive sensing-member materials are given. It is shown that all practical sensors include some means of compensation to average out characteristic nonideal properties of magnetostrictive sensing materials.</div>
cGMP-dependent protein kinase activates Ca-activated K channels in cerebral artery smooth muscle cells (1993)

Guanosine-3',5'-cyclic monophosphate (cGMP)-dependent protein kinase (cGMP-PK) plays a central role in the mediation of the vasodilator response to nitric oxide (NO) and other nitrovasodilators. It is unclear whether cGMP-PK affects calcium-activated potassium channels (KCa channels) or any other type of ion channel in smooth muscle. We provide here the first direct evidence that cGMP-PK can activate KCa channels in arterial smooth muscle cells. We demonstrate that NO and a membrane-permeable analogue of cGMP can activate KCa channels in on-cell patches approximately twofold. Furthermore, cGMP-PK, in the presence of ATP and cGMP added directly to the intracellular surface of inside-out patches, increases channel activity by approximately eightfold. These results suggest that cGMP-PK-mediated activation of KCa channels may contribute to the actions of NO and other nitrovasodilators.
Determination of<scp>l</scp>-Phenylalanine Based on an NADH-Detecting Biosensor (1998)

An enzyme carbon paste electrode containing three different enzymes was developed for the determination of L-phenylalanine. This sensor is based on the enzymatic/electrochemical recycling of tyrosinase in combination with salicylate hydroxylase and L-phenylalanine dehydrogenase (PADH). The enzymes salicylate hydroxylase and tyrosinase were coimmobilized first in a carbon paste electrode for the sensitive detection of NADH. The principle of the bienzyme scheme is as follows: the first enzyme, salicylate hydroxylase, converts salicylate to catechol in the presence of oxygen and NADH. The second enzyme, tyrosinase, then oxidizes the catechol to o-quinone, which is electrochemically detected and reduced back to catechol at the electrode at an Eappl = -50 mV vs Ag/AgCl. This results in an amplified signal due to the recycling of the catechol and o-quinone between tyrosinase and the surface of the electrode. Prior to adding PADH, the salicylate hydroxylase-tyrosinase carbon paste electrode was characterized in terms of its sensitivity to NADH, pH dependence, buffer composition, interferences, and stability. Interference from ascorbic acid and uric acid was found to be minimal. Human serum was used to investigate whether this bienzyme system was suitable for the detection of NADH in serum and blood samples. The sensitivity for NADH was increased by a factor of 33 times using the bienzyme amplification scheme (electroreduction of o-quinone at Eappl = -50 mV) as opposed to the salicylate hydroxylase single-enzyme system (at which catechol would have been oxidized at Eappl = +150 mV vs Ag/AgCl). The detection limit for NADH achieved by the bienzyme carbon paste electrode was 1 vs 100 microM for the single-enzyme carbon paste electrode. The salicylate hydroxylase-tyrosinase system was then coupled with phenylalanine dehydrogenase for L-phenylalanine determination. This multienzyme sensor was able to achieve a linear range of 20-150 microM and a detection limit of 5 microM for L-phenylalanine. The sensitivity is sufficient since the reference clinical range for L-phenylalanine is 78-206 microM.
Enhanced Random Access: Initial access load balance in highly dense LTE-A networks for multiservice (H2H-MTC) traffic (2017)

The Random Access (RA) procedure in existing cellular networks is not capable of functioning properly during high access load conditions. For this purpose, overload control mechanisms are needed. Most proposed mechanisms in the literature offer a tradeoff between access rate and experienced delay. However, when the maximal tolerated delay and the energy spent on retransmissions are tightly bounded, the very high access rate, targeted for 5G systems, cannot be achieved. For these situations, we propose the Delay Estimation based RA (DERA)-scheme that has the potential to meet very stringent reliability requirements, even in high access load conditions. The present work shows that this goal can be achieved only in the cost of limited additional complexity. Furthermore, we also study the optimal switchover point at which the proposed scheme moves from low-load to the high-load phase. The derived tool can also be used along with other proposed RA overload control schemes, e.g. when to invoke access class barring. The performance evaluation results show that the novel DERA scheme can significantly improve the control channels' resource utilization along with the success rate in dense deployment scenarios.
Regulation of pre‐B cell proliferation in bone marrow: immunofluorescence stathmokinetic studies of cytoplasmic p chain‐bearing cells in anti‐IgM‐treated mice, hematologically deficient mutant mice and mice given sheep red blood cells (1985)

To identify factors influencing the in vivo proliferate activity of bone marrow pre-B cells, the metaphase-blocking drug, vincristine sulfate, was injected into (a) mice depleted of B lymphocytes by treatment with anti-mouse IgM antibodies from birth; (b) hematologically deficient W/Wv and Sl/Sld mutants, and (c) mice injected with a foreign agent, sheep red blood cells (SRBC). Subsequently, a quantitative measure of pre-B cell proliferation was provided by examining marrow cells by immunofluorescence labeling for the absolute number of pre-B cells, identified by the presence of cytoplasmic mu chains (c mu) without surface mu (s mu), which had been arrested in metaphase. In anti-IgM-treated mice, some changes were observed in the size of the large pre-B cell population and in the incidence of mitotic cells after vincristine administration, but the overall production rate of pre-B cells did not differ from that in controls given normal rabbit serum. Pre-B cell kinetics in W/Wv and Sl/Sld mice also generally resembled those in homozygous controls. In contrast, after SRBC injection, there was an increase in the rate at which large pre-B cells entered mitosis. Thus, the proliferation of c mu + s mu- bone marrow pre-B cells shows no evidence of feedback control from the mature B lymphocyte pool, as indicated by lack of stimulation of pre-B cell production in anti-IgM-treated mice, and is independent of the hemopoietic defects of W/Wv or Sl/Sld mutants. On the other hand, the increased bone marrow pre-B cell proliferation after SRBC injection demonstrates that the magnitude of B cell genesis in the bone marrow can be influenced by extrinsic agents and thus may be influenced by environmental stimuli.