gebhart/patrepeval huggingface dataset:
The proliferation potential protein-related (P2P-R) gene with domains encoding heterogeneous nuclear ribonucleoprotein association and Rb1 binding shows repressed expression during terminal differentiation (1997)

Terminal differentiation is associated with repression in the expression of the proliferation potential proteins (P2P) subset of heterogeneous nuclear ribonucleoprotein (hnRNP) proteins. We report here the cloning and characterization of a 5173-bp P2P-related (P2P-R) cDNA that contains a 4214-bp open reading frame. Probes to this cDNA detect a single 8-kb mRNA in multiple murine tissues and in proliferating 3T3T cells, but not in terminally differentiated 3T3T adipocytes. Evidence that this cDNA can encode peptides with domains for hnRNP association was established by showing that such peptides are recognized by two monoclonal antibodies known to detect core hnRNP proteins, and by showing that the C130 monoclonal antibody, produced against a cDNA-derived fusion protein, also selectively detects native P2P hnRNP proteins. In addition, P2P-R cDNA-derived fusion proteins bind single-stranded nucleic acids, and a P2P-R cDNA-derived antisense oligonucleotide selectively represses P2P expression. Because terminal differentiation is associated with modulation in Rb1 function, we assayed if products of this cDNA might interact with Rb1. Evidence that the P2P-R cDNA encodes a protein domain that binds Rb1 was established using a glutathione S-transferase fusion protein to selectively precipitate Rb1 from cellular extracts. Data also show that this binding is reduced by competition with the adenovirus E1a protein, indicating that binding occurs through the "pocket" domain of Rb1. These results establish that the P2P-R cDNA encodes protein domains involved in both hnRNP association and Rb1 binding and complement recent reports that localize Rb1 to sites of RNA processing in the nucleus.
ATR-FTIR spectral discrimination between normal and tumorous mouse models of lymphoma and melanoma from serum samples (2017)

This study presents, attenuated total reflection Fourier transforms infrared spectroscopy of dried serum samples in an effort to assess biochemical changes induced by non-Hodgkin's lymphoma and subcutaneous melanoma. An EL4 mouse model of non-Hodgkin lymphoma and a B16 mouse model of subcutaneous melanoma are used to extract a snapshot of tumor-associated alteration in the serum. The study of both cancer-bearing mouse models in wild types and their corresponding control types, emphasizes the diagnostic potential of this approach as a screening technique for non-Hodgkin lymphoma and melanoma skin cancer. Infrared absorbance values of the different spectral bands, hierarchical clustering and integral values of the component bands by curve fitting, show statistically significant differences (student's t-test, two-tailed unequal variance p-value < 0.05) between spectra representing healthy and tumorous mouse. This technique may thus be useful for having individualized route maps for rapid evaluation of lymphoma and melanoma status and associated therapeutic modalities.
The use of honey as an antiseptic in managing <i>Pseudomonas</i> infection (1999)

A laboratory study was undertaken to extend existing knowledge about the effectiveness of the antibacterial properties of honey against pseudomonads. To date, sensitivity testing has used non-standardised honeys, which may vary greatly in their antibacterial potency. Pure cultures of Pseudomonas spp, isolated from swabs from 20 infected wounds, were inoculated on the surface of nutrient agar plates containing various concentrations of honey in the medium. Two types of honey were used, a manuka honey and a pasture honey, each selected to have antibacterial activity close to the median for each type. The minimum inhibitory concentration of the manuka honey for the 20 isolates ranged from 5.5-8.7% (v/v) (mean 6.9% (v/v), standard deviation 1.3). The minimum inhibitory concentration of the pasture honey for the 20 isolates ranged from 5.8-9.0% (v/v) (mean 7.1% (v/v), standard deviation 1.0). Honeys with an average level of antibacterial activity could be expected to be effective in preventing the growth of pseudomonads on the surface of a wound even if the honey were diluted more than ten-fold by exudation from the wound.
A divide-and-merge methodology for clustering (2005)

We present a divide-and-merge methodology for clustering a set of objects that combines a top-down "divide" phase with a bottom-up "merge" phase. In contrast, previous algorithms either use top-down or bottom-up methods to construct a hierarchical clustering or produce a flat clustering using local search (e.g., k-means). Our divide phase produces a tree whose leaves are the elements of the set. For this phase, we use an efficient spectral algorithm. The merge phase quickly finds an optimal tree-respecting partition for many natural objective functions, e.g., k-means, min-diameter, min-sum, correlation clustering, etc., We present a meta-search engine that uses this methodology to cluster results from web searches. We also give empirical results on text-based data where the algorithm performs better than or competitively with existing clustering algorithms.
D<sub>2</sub>-m-Carborane Siloxanes. 7. Synthesis and Properties of Ultra-High Molecular Weight Polymer (1979)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTD2-m-Carborane Siloxanes. 7. Synthesis and Properties of Ultra-High Molecular Weight PolymerDonald D. Stewart, Edward N. Peters, C. D. Beard, G. B. Dunks, E. Hedaya, G. T. Kwiatkowski, R. B. Moffitt, and J. J. BohanCite this: Macromolecules 1979, 12, 3, 373–377Publication Date (Print):May 1, 1979Publication History Published online1 May 2002Published inissue 1 May 1979https://pubs.acs.org/doi/10.1021/ma60069a004https://doi.org/10.1021/ma60069a004research-articleACS PublicationsRequest reuse permissionsArticle Views305Altmetric-Citations25LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
A Microfabricated Platform to Measure and Manipulate the Mechanics of Engineered Cardiac Microtissues (2011)

Engineered myocardial tissues can be used to elucidate fundamental features of myocardial biology, develop organotypic in vitro model systems, and as engineered tissue constructs for replacing damaged heart tissue in vivo. However, a key limitation is an inability to test the wide range of parameters (cell source, mechanical, soluble and electrical stimuli) that might impact the engineered tissue in a high-throughput manner and in an environment that mimics native heart tissue. Here we used microelectromechanical systems technology to generate arrays of cardiac microtissues (CMTs) embedded within three-dimensional micropatterned matrices. Microcantilevers simultaneously constrain CMT contraction and report forces generated by the CMTs in real time. We demonstrate the ability to routinely produce ~200 CMTs per million cardiac cells (<1 neonatal rat heart) whose spontaneous contraction frequency, duration, and forces can be tracked. Independently varying the mechanical stiffness of the cantilevers and collagen matrix revealed that both the dynamic force of cardiac contraction as well as the basal static tension within the CMT increased with boundary or matrix rigidity. Cell alignment is, however, reduced within a stiff collagen matrix; therefore, despite producing higher force, CMTs constructed from higher density collagen have a lower cross-sectional stress than those constructed from lower density collagen. We also study the effect of electrical stimulation on cell alignment and force generation within CMTs and we show that the combination of electrical stimulation and auxotonic load strongly improves both the structure and the function of the CMTs. Finally, we demonstrate the suitability of our technique for high-throughput monitoring of drug-induced changes in spontaneous frequency or contractility in CMTs as well as high-speed imaging of calcium dynamics using fluorescent dyes. Together, these results highlight the potential for this approach to quantitatively demonstrate the impact of physical parameters on the maturation, structure, and function of cardiac tissue and open the possibility to use high-throughput, low volume screening for studies on engineered myocardium.
High speed high quality antialiased vector generation (1990)

A vector generation method is described in which a high quality image rendering scheme is coupled with a high speed scan-conversion algorithm.The rendering scheme consists of two parts. First a prefiltering method is used to antialias the vectors. Second a compositing technique is used to compose the vectors into the frame-buffer.The scan-conversion algorithm presented allows a single vector to be scan-converted by a either by a single processor or a set of processors running in parallel. When using parallel processors, antialiased vectors may be scan-converted and written to a frame store at the same high speed as aliased vectors. The VLSI technology used to implement this algorithm is capable of drawing over two million high quality antialiased vectors per second.
Towards New Antifolates Targeting Eukaryotic Opportunistic Infections (2009)

Trimethoprim, an antifolate commonly prescribed in combination with sulfamethoxazole, potently inhibits several prokaryotic species of dihydrofolate reductase (DHFR). However, several eukaryotic pathogenic organisms are resistant to trimethoprim, preventing its effective use as a therapeutic for those infections. We have been building a program to reengineer trimethoprim to more potently and selectively inhibit eukaryotic species of DHFR as a viable strategy for new drug discovery targeting several opportunistic pathogens. We have developed a series of compounds that exhibit potent and selective inhibition of DHFR from the parasitic protozoa Cryptosporidium and Toxoplasma as well as the fungus Candida glabrata. A comparison of the structures of DHFR from the fungal species Candida glabrata and Pneumocystis suggests that the compounds may also potently inhibit Pneumocystis DHFR.
α-Phosphonosulfonic Acids:  Potent and Selective Inhibitors of Squalene Synthase (1996)

ADVERTISEMENT RETURN TO ISSUEPREVLetterNEXTα-Phosphonosulfonic Acids: Potent and Selective Inhibitors of Squalene SynthaseDavid R. Magnin, Scott A. Biller, Ying Chen, John K. Dickson,, Olga M. Fryszman, R. Michael Lawrence, Janette V. H. Logan, Ellen S. Sieber-McMaster, Richard B. Sulsky, Sarah C. Traeger, Dolly C. Hsieh, Shih-Jung Lan, J. Kent Rinehart, Thomas W. Harrity, Kern G. Jolibois, Lori K. Kunselman, Lois C. Rich, Dorothy A. Slusarchyk, and Carl P. CiosekView Author Information Bristol-Myers Squibb Pharmaceutical Research Institute, P.O. Box 4000, Princeton, New Jersey 08543 Cite this: J. Med. Chem. 1996, 39, 3, 657–660Publication Date (Web):February 2, 1996Publication History Received5 October 1995Published online2 February 1996Published inissue 1 January 1996https://pubs.acs.org/doi/10.1021/jm9507340https://doi.org/10.1021/jm9507340rapid-communicationACS PublicationsCopyright © 1996 American Chemical SocietyRequest reuse permissionsArticle Views445Altmetric-Citations33LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Anatomy,Aromatic compounds,Hydrocarbons,Inhibitors,Rodent models Get e-Alerts
Image guidance based on MRI for spinal interstitial laser thermotherapy: technical aspects and accuracy (2017)

OBJECTIVE Image guidance for spinal procedures is based on 3D-fluoroscopy or CT, which provide poor visualization of soft tissues, including the spinal cord. To overcome this limitation, the authors developed a method to register intraoperative MRI (iMRI) of the spine into a neuronavigation system, allowing excellent visualization of the spinal cord. This novel technique improved the accuracy in the deployment of laser interstitial thermal therapy probes for the treatment of metastatic spinal cord compression. METHODS Patients were positioned prone on the MRI table under general anesthesia. Fiducial markers were applied on the skin of the back, and a plastic cradle was used to support the MRI coil. T2-weighted MRI sequences of the region of interest were exported to a standard navigation system. A reference array was sutured to the skin, and surface matching of the fiducial markers was performed. A navigated Jamshidi needle was advanced until contact was made with the dorsal elements; its position was confirmed with intraoperative fluoroscopy prior to advancement into a target in the epidural space. A screenshot of its final position was saved, and then the Jamshidi needle was exchanged for an MRI-compatible access cannula. MRI of the exact axial plane of each access cannula was obtained and compared with the corresponding screenshot saved during positioning. The discrepancy in millimeters between the trajectories was measured to evaluate accuracy of the image guidance RESULTS Thirteen individuals underwent implantation of 47 laser probes. The median absolute value of the discrepancy between the location predicted by the navigation system and the actual position of the access cannulas was 0.7 mm (range 0-3.2 mm). No injury or adverse event occurred during the procedures. CONCLUSIONS This study demonstrates the feasibility of image guidance based on MRI to perform laser interstitial thermotherapy of spinal metastasis. The authors' method permits excellent visualization of the spinal cord, improving safety and workflow during laser ablations in the epidural space. The results can be extrapolated to other indications, including biopsies or drainage of fluid collections near the spinal cord.
Specimen analysis instrument assembly (1989)

Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter Facebook Reddit LinkedIn Tools Icon Tools Reprints and Permissions Cite Icon Cite Search Site Citation John W. Clayland, Carl P. Daniel; Specimen analysis instrument assembly. J. Acoust. Soc. Am. 1 August 1989; 86 (2): 863–864. https://doi.org/10.1121/1.398124 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAcoustical Society of AmericaThe Journal of the Acoustical Society of America Search Advanced Search |Citation Search
Intrasystem interconnection in telecommunication platforms using plastic optical fiber (2002)

Large telecommunication switching and transmission platforms have massive interconnection requirements which are in the 1 Tb/s range and growing rapidly. Plastic optical fiber technology shows promise as a replacement for both metallic interconnection and glass optical fiber interconnection. The high bandwidth characteristics of graded index plastic optical fiber (GI POF) are particularly attractive in these applications which require high reliability and high bandwidth interconnection over short distances.
Antibodies to the Ventral Disc Protein δ-giardin Prevent in Vitro Binding of Giardia lamblia Trophozoites (2009)

A cDNA coding for detlaq-giardin was cloned from Giardia lamblia trophozoites to localize the protein and to study its function in mediating surface attachment. Recombinant delta-giardin antigen was expressed in Escherichia coli as a poly-histidine fusion protein and was purified by affinity chromatography for production of antisera to delta-giardin. By immunoblotting analysis, antisera to recombinant delta-giardin antigen recognized a 31-kDa protein on G. lamblia trophozoites. Anti-recombinant delta-giardin was used to localize the native protein to the trophozoite ventral disk in both immunofluorescence and immunoelectron microscopy assays. Pre-treatment of G. lamblia trophozoites with anti-delta-giardin sera caused morphological changes in the parasite and inhibited trophozoite binding to the surface of cell culture slides. Binding of antibodies to delta-giardin may provide a means of inhibiting attachment of G. lamblia trophozoites to the intestinal epithelium and thereby prevent clinical giardiasis.
Gamut Calculation of Color Reproduction Devices (1996)

More advanced color management systems rely on the availability of the gamut description of a color reproduction device. The problem of calculating such a gamut description, however, has no definitive solution yet.In this paper we explain that several kinds of surfaces exist in colorant space, that map to the gamut boundaries in color space. The different kinds of gamut boundaries that should be taken into account do not only depend on the mathematical model that describes the color mixing behavior of the reproduction device, but also depend on the colorant domain (e.g. colorant limitation) and the number of colorants of the printer.The color gamut boundaries can be determined mathematically for any printer model and any number of inks. For some models however, such as the Neugebauer equations, most kinds of gamut boundaries can be described analytically.
Laboratory-Scale Characterization of a Green Sorption Medium for On-Site Sewage Treatment and Disposal to Improve Nutrient Removal (2010)

On-site sewage treatment and disposal systems (OSTDSs) are oftentimes used to treat human waste accruing from nearly one-third of the U.S. population. Operation of OSTDS contributes a large fraction of nutrient loads to groundwater aquifers. This article aims to develop and present a new “green sorption medium,” which has not been fully tested before, to be placed in the vadose zone of the OSTDS underground drain fields. This green sorption medium is comprised of recycled materials with “green” implications for nutrient absorption and adsorption. The composition or recipe of the new material mix was derived based on a thorough literature review. Technical coverage of this study includes material characterization, batch isotherm identification, and a microcosm study leading to improve the application potential of these new material mixes. Batch absorption isotherm data was well fitted by the Langmuir and Freundlich isotherm models. Under the hydraulic retention time of 24 h in a laboratory-scale microcosm of unique functional design, removal efficiencies were found significant for all pollutants of concern. Running as a continuous system, the microcosm, filled with the preselected recipes, was dosed with septic effluent containing 1.76 mg L−1 of ammonia, 0.166 mg L−1 of nitrite, 0.352 mg L−1 of nitrate, and 1.498 mg L−1 of orthophosphorus. This study confirmed 55% removal efficiency of nitrates and 89% of phosphorus under a hydraulic retention time of 24 h. With the aid of Langmuir isotherm data, the theoretical life expectancy of the proposed sorption media mix used in the microcosm was estimated to be 1.2 years for phosphorus sorption. Results obtained largely complement current knowledge on the use of green sorption media for nutrient removal. These findings should also elaborate full-scale field studies in the future.
Microphone Array Post-Filter using Incremental Bayes Learning to Track the Spatial Distributions of Speech and Noise (2007)

While current post-filtering algorithms for microphone array applications can enhance beamformer output signals, they assume that the noise is either incoherent or diffuse, and make no allowances for point noise sources which may be strongly correlated across the microphones. In this paper, we present a novel post-filtering algorithm that alleviates this assumption by tracking the spatial as well as spectral distribution of the speech and noise sources present. A generative statistical model is employed to model the speech and noise sources at distinct regions in the soundfield, and incremental Bayesian learning is used to track the model parameters over time. This approach allows a post-filter derived from these parameters to effectively suppress both diffuse ambient noise and interfering point sources. The performance of the proposed approach is evaluated on multiple recordings made in a realistic office environment.
Cubic AgPb <i> <sub>m</sub> </i> SbTe <sub>2+</sub> <i> <sub>m</sub> </i> : Bulk Thermoelectric Materials with High Figure of Merit (2004)

The conversion of heat to electricity by thermoelectric devices may play a key role in the future for energy production and utilization. However, in order to meet that role, more efficient thermoelectric materials are needed that are suitable for high-temperature applications. We show that the material system AgPb(m)SbTe(2+m) may be suitable for this purpose. With m = 10 and 18 and doped appropriately, n-type semiconductors can be produced that exhibit a high thermoelectric figure of merit material ZTmax of approximately 2.2 at 800 kelvin. In the temperature range 600 to 900 kelvin, the AgPb(m)SbTe(2+m) material is expected to outperform all reported bulk thermoelectrics, thereby earmarking it as a material system for potential use in efficient thermoelectric power generation from heat sources.
A technique for projection x-ray lithography using computer-generated holograms (1992)

X-ray projection lithography has recently been explored as a method for the manufacture of &lt;200 nm linewidth integrated circuits. A method is described whereby projected lithographic images can be formed with x rays by means of a transmission hologram. The form of the hologram is computed by an algorithm that eliminates the unwanted signals normally present as systematic errors in in-line holographic images. Example calculations are shown in which holograms are constructed to generate images of a given test pattern. The holographic images are shown to have excellent fidelity with respect to both resolution and contrast. Such an approach to projection x-ray lithography requires an x-ray beam with very little coherence and is thus compatible with high wafer throughput schemes; in addition, image fidelity remains high even when moderately small contaminant particles block the light from small regions of the hologram. Fabrication limits for the single optic involved are compatible with current technological limits. The approach is applicable over a large range of soft-x-ray wavelengths, indicating the feasibility of a 100 nm resolution system with 6 μm depth of field using a carbon mask system operating at 5 nm, or other materials at shorter wavelengths.