gebhart/patrepeval huggingface dataset:
Pyrithiamine Resistance Gene (<i>ptrA</i>) of<i>Aspergillus oryzae</i>: Cloning, Characterization and Application as a Dominant Selectable Marker for Transformation (2000)

A pyrithiamine (PT) resistance gene (ptrA) was cloned from a genomic DNA library prepared from a PT resistant mutant of Aspergillus oryzae. It conferred high resistance to PT on an A. oryzae industrial strain as well as A. nidulans. Nucleotide sequence analysis showed that the ptrA gene contained one intron (58-bp) and encodes 327 amino acid (aa) residues. Additionally, the deduced aa sequence has 72% and 63% identity to Fusarium solani sti35 encoding a stress-inducible protein and Saccharomyces cerevisiae THI4 encoding an enzyme involved in thiamine biosynthesis, respectively, indicating that ptrA is a mutated allele of a gene belonging to the THI4 family. The mutation point was identified in the conserved motif in 5'-flanking region of these three THI4 homologous genes (ptrA, sti35, and THI4). The introduction of the ptrA gene allowed an A. oryzae industrial strain to grow on the minimum medium containing PT (0.1 mg/l) on which an untransformed strain did not grow. This result indicates that the ptrA is applicable as a dominant selectable marker for transformation of A. oryzae.
Whole-Genome Sequence of Streptococcus suis Serotype 3 Strain YB51 (2013)

We report here the second complete genome sequence of Streptococcus suis serotype 3 (strain YB51). The genome is 2,043,655 bp in length, which is 14,840 bp longer than the first reported genome of the same serotype, and it covers 2,012 coding sequences, 56 tRNAs, and 4 rRNA loci.
ChemInform Abstract: BILE ACID DERIVATIVES WITH ANTIMICROBIAL ACTIVITY (1980)

A series of condensation products of cholic and dehydrocholic acids with (L)-aminoacids was prepared and tested in vitro for antimicrobial activity. The derivatives of cholic acid with basic aminoacids showed significant activity, especially marked when (L)-arginine was the condensed aminoacid.
Development of a High-Performance Wind Turbine Equipped with a Brimmed Diffuser Shroud (2006)

We have developed a new wind turbine system that consists of a diffuser shroud with a broad-ring brim at the exit periphery and a wind turbine inside it. The brimmed-diffuser shroud plays the role of a device for collecting and accelerating the approaching wind. Emphasis is placed on positioning the brim at the exit of the diffuser shroud. Namely, the brim generates a very low-pressure region in the exit neighborhood of the diffuser by strong vortex formation and draws more mass flow to the wind turbine inside the diffuser shroud. To obtain a higher power output of the shrouded wind turbine, we have examined the optimal form for the brimmed diffuser, such as the diffuser open angle, brim height, hub ratio, centerbody length, inlet shroud shape and so on. As a result, a shrouded wind turbine equipped with a brimmed diffuser has been developed, and demonstrated power augmentation for a given turbine diameter and wind speed by a factor of about five compared to a standard (bare) wind turbine.
Quantitative investigation of reproduction of gonosomal condensed chromatin during trophoblast cell polyploidization and endoreduplication in the east-european field vole Microtus rossiaemeridionalis (2003)

Simultaneous determinations of DNA content in cell nuclei and condensed chromatin bodies formed by heterochromatized regions of sex chromosomes (gonosomal chromatin bodies, GCB) have been performed in two trophoblast cell populations of the East-European field vole Microtus rossiaemeridionalis: in the proliferative population of trophoblast cells of the junctional zone of placenta and in the secondary giant trophoblast cells. One or two GCBs have been observed in trophoblast cell nuclei of all embryos studied (perhaps both male and female). In the proliferative trophoblast cell population characterized by low ploidy levels (2-16c) and in the highly polyploid population of secondary giant trophoblast cells (32-256c) the total DNA content in GCB increased proportionally to the ploidy level. In individual GCBs the DNA content also rose proportionally to the ploidy level in nuclei both with one and with two GCBs in both trophoblast cell populations. Some increase in percentage of nuclei with 2-3 GCBs was shown in nuclei of the placenta junctional zone; this may be accounted for by genome multiplication via uncompleted mitoses. In nuclei of the secondary giant trophoblast cells (16-256c) the number of GCBs did not exceed 2, and the fraction of nuclei with two GCBs did not increase, which suggests the polytene nature of sex chromosomes in these cells. In all classes of ploidy the DNA content in trophoblast cell nuclei with the single GCB was lower than in nuclei with two and more GCBs. This can indicate that the single GCB in many cases does not derive from fusion of two GCBs. The measurements in individual GCBs suggest that different heterochromatized regions of the X- and Y-chromosome may contribute in GCB formation.
Screen and Roll: Transmissions of Embodied Knowledge through Canadian Women’s Basketball History (2017)

Analyzing the role of cockfights in Balinese culture, Clifford Geertz argues, “Attending cockfights and participating in them is, for the Balinese, a kind of sentimental education. What he learns there is what his culture’s ethos and his private sensibility (or anyway, certain aspects of them) look like when spelled out externally in a collective text” (449). Geertz’s thesis can be applied to Western sport: particularly in the twentieth century, sport was a place where men went to learn physical skills, values, and sentiments associated with masculinity. In this article, I examine the residue of sport’s male-dominated history through performative writing. I weave together historical quotations, performance theory, and a personal narrative about my experiences as a female basketball player and coach to consider the relationship between female bodies, the practice of sport, and the transmission of embodied knowledge. Ultimately, I argue that embodied repertoire is a significant site for the transmission of sport technique and the study of sport history.
Reforming the House of Lords (2000)

Abstract The House of Lords has been the subject of numerous studies. Its reform has been discussed on and off for most of this century. It is a remarkable institution, with a remarkable history, which has been ably described by many others. The focus of this book, however, is the work of second chambers overseas, and what we can learn from this for the reform of the UK upper house. Before embarking on that overseas survey, this chapter runs very briefly through the essential features of the House of Lords — its composition, its work, a brief history of the previous attempts at reform, and the new constitutional context in which the latest reforms are taking place.
Optical filter requirements in an EML-based single-sideband PAM4 intensity-modulation and direct-detection transmission system (2017)

The feasibility of a single sideband (SSB) PAM4 intensity-modulation and direct-detection (IM/DD) transmission based on a CMOS ADC and DAC is experimentally demonstrated in this work. To cost effectively build a >50 Gb/s system as well as to extend the transmission distance, a low cost EML and a passive optical filter are utilized to generate the SSB signal. However, the EML-induced chirp and dispersion-induced power fading limit the requirements of the SSB filter. To separate the effect of signal-signal beating interference, filters with different roll-off factors are employed to demonstrate the performance tolerance at different transmission distance. Moreover, a high resolution spectrum analysis is proposed to depict the system limitation. Experimental results show that a minimum roll-off factor of 7 dB/10GHz is required to achieve a 51.84Gb/s 40-km transmission with only linear feed-forward equalization.
Thin Film Microtransformer Integrated on Silicon for Signal Isolation (2007)

Microtransformers have been fabricated on silicon substrates with the aim of providing isolation for signal and power. Interleaved primary and secondary windings are sandwiched between two electroplated magnetic layers. The transformer has a turn ratio of 4:4. It has a primary inductance of 400 nH at low frequencies and dc resistance of 0.48 Omega. The voltage gain is -1 dB between 1-20 MHz with a 50-Omega load. When compared to previously reported microtransformer characteristics this is the highest reported voltage gain for a microtransformer
Isolation of insect pathogenic bacteria, <i>Providencia rettgeri</i> , from <i>Heterorhabditis</i> spp. (1995)

Nematodes of the genus Heterorhabditis carry bacteria of the genus Photorhabdus into insects including pests of horticultural crops. The bacteria kill the insect and provide conditions which allow for the growth and development of the nematodes. It is reported here that the majority of Heterorhabditis spp. strains tested contained a second bacterial species which was identified as Providencia rettgeri. Injection of the bacteria into waxmoth larvae showed that P. rettgeri was at least as pathogenic as Photorhabdus sp. K122. Both had LD 50 values of less than one bacterial cell/larva, but P. rettgeri killed the insects at a considerably faster rate than K122 at both 28°C and 9°C. Since Photorhabdus kills very slowly at low temperatures, it appeared that P. rettgeri might be a better pest control agent under these conditions. However, P. rettgeri was not pathogenic when carried into insect larvae by the nematode, indicating that the nematode suppressed either its release or pathogenicity. It will be necessary to find ways of bypassing or inhibiting this suppression for P. rettgeri to fulfil its potential in pest control.
Allergen-specific T-cell Response in Patients with Phenytoin Hypersensitivity; Simultaneous Analysis of Proliferation and Cytokine Production by Carboxyfluorescein Succinimidyl Ester (CFSE) Dilution Assay (2007)

Phenytoin-specific proliferation may be detected with greater sensitivity by the CFSE dilution assay than the conventional LTT. The assay revealed that both CD4+ and CD4- T cells proliferated and produced IFN-gamma and TNF-alpha after stimulation with phenytoin. The CFSE dilution assay might be useful for the diagnosis and understanding of drug hypersensitivity.
Strategies for the Discovery of Secondary Metabolites from Marine Bacteria: Ecological Perspectives (1994)

Human coronavirus (HCoV) infection causes respiratory diseases with mild to severe outcomes. In the last 15 years, we have witnessed the emergence of two zoonotic, highly pathogenic HCoVs: severe acute respiratory syndrome coronavirus (SARS-CoV) and ...Read More
Flammable Liquids (2000)

ADVERTISEMENT RETURN TO ISSUEPREVAnecdotal AccidentsNEXTFlammable LiquidsJay A YoungJay A YoungMore by Jay A YoungCite this: Chem. Health Saf. 2000, 7, 5, 4Publication Date (Web):September 1, 2000Publication History Published online1 September 2000Published inissue 1 September 2000https://doi.org/10.1016/S1074-9098(00)00138-6Copyright © 2000 RIGHTS & PERMISSIONSArticle Views9Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit Read OnlinePDF (185 KB) Get e-Alerts
Electrical and Mechanical Properties of Semi-Conductive Sheath Materials Aged at High Temperatures in Seawater (2006)

Direct electrical heating (DEH) has shown to be a very promising method to prevent wax and hydrate formation in subsea pipelines in the North Sea. In this system the heated pipeline is an active conductor in a single-phase AC electric circuit, together with a single core high voltage extruded cable strapped (piggybacked) to the heated flowline. For new projects with flowlines exceeding 40 km, the insulating outer sheath and the metallic ground screens can be replaced by a semi-conductive outer sheath for a continuous transfer of capacitive currents to seabed, or to clay for buried sections. Due to the relative high temperatures of the crude oil, thermal insulating properties of the clay and heating of the cable conductor, relative high temperatures can be subjected to the cable materials. This paper presents results from measurements of mechanical and electrical properties of different candidate semi-conductive cable outer sheath materials aged in relevant and accelerated service conditions, and measurements of electrical resistivity and water content of clay samples taken from relevant oil fields. The tests also show that approximately the same resistivity was measured for the semi-conductive outer sheath and clay making continuously transferring of AC currents to ground possible.
Long-term efficacy of adalimumab in the treatment of uveitis associated with juvenile idiopathic arthritis (2011)

Adalimumab is a valuable option in the treatment of uveitis associated with active juvenile idiopathic arthritis.
Upper Limit of CHF in the Saturated Forced Convection Boiling on a Heated Disk with a Small Impinging Jet (1979)

Critical heat flux (CHF) in the forced convection boiling on an open heated disk being supplied with saturated liquids through a small high-speed jet impinging at the center of disk is studied experimentally employing Refrigerant 12 at comparatively high pressures from 6.0 to 27.9 bars as well as water and Refrigerant 113 at atmospheric pressure. Generalized correlations of CHF are obtained for two characteristic regimes: V-regime where CHF is variable and I-regime where CHF is invariable for the change of jet velocity. Then, the boundaries of each regime are discussed clarifying the aspects for the lower limit of jet velocity capable of generating I-regime as well as the upper limit of CHF.
Typing of porcine reproductive and respiratory syndrome viruses by a multiplex PCR assay (1997)

A rapid multiplex PCR assay was developed to distinguish between North American and European genotypes of porcine reproductive and respiratory syndrome (PRRS) virus after a portion of the polymerase gene (open reading frame 1b) was sequenced for two North American PRRS virus strains. DNA products with unique sizes characteristic of each genotype were obtained.
Tear-Me-Open Software License Agreements: A Uniform Commercial Code Perspective on an Innovative Contract of Adhesion, 7 Computer L.J. 261 (1986) (1986)

The introduction of powerful low-cost microcomputers has resulted in the development and mass marketing of inexpensive, ready to use software by the software industry for use in personal computers.'The market for such software is large, lucrative, and rapidly expanding. 2 In order to protect intellectual property rights in the software they produce, 3 and to limit their liability for defective software, software developers are attempting to establish contracts which will govern the software transactions between themselves and their customers in the * National Third Place, Third Annual Computer Law Writing Competition.confusion in this regard.9. Some agreements prohibit any transfer of the software to third parties.See
A survey of asynchronous remote procedure calls (1992)

Remote Procedure Call (RPC) is a popular paradigm for interprocess communication in distributed systems. It is simple, flexible and powerful. However, most of the RPC systems today are synchronous in nature, and hence fail to exploit fully the parallelism inherent in distributed applications. In view of this, various asynchronous RPC systems have been designed and implemented to achieve higher parallelism while retaining the familiarity and simplicity of synchronous RPC. Asynchronous RPC calls do not block the caller (client) and the replies can be received as and when they are needed, thus allowing the client execution to proceed locally in parallel with the callee (server) invocation. Asynchronous RPC calls can be classified into two types depending on whether the calls return a value. Most asynchronous RPC systems only support calls that do not return a value, and few support both classes. In this paper, an analysis and comparison of various asynchronous RPC systems are presented.
Quantitative absorption spectroscopy and dissociation dynamics of I2 van der Waals complexes with He, Ar, Kr, and Xe (1986)

Noble gas (He, Ar, Kr, and Xe) van der Waals (vdW) complexes of I2 formed in axiosymmetric, pulsed jet expansions were studied by absorption using intracavity laser spectroscopy (ILS). Absorption spectra of I2He, I2Ar, I2Ar2, and I2Ar3 as well as previously undetected I2Kr, I2Kr2, I2Kr3, I2Xe, and I2Xe2 were recorded using B(3Π+0u)←X transitions (Figs. 2–4 and Table I). The formation efficiencies of nine I2–noble gas vdW complexes were studied as a function of expansion conditions (Table II). Comparisons of absorption and laser-induced fluorescence data provide an analysis of excited-state dissociation mechanisms for the overall He, Ar, Kr, and Xe chemical series and as a function of vibrational level populated in the B(3Π+0u) state for I2He and I2Ar. The competition between the electronic and vibrational predissociation of the vdW complex is discussed with respect to absorption data.
A Convenient One-Pot Preparation of Disubstituted Phosphinic Acids Derived from Simple Amino Acids and Proline (1995)

A facile method for the preparation of both 2-pyrrolidinyl-substituted phosphinic acids and 1-aminoalkylphosphinic acids is described. Reaction of 1-pyrroline trimer or N-tritylalkanamine with bis(trimethylsilyl)phosphonite, followed by silylation with N,O-bis(trimethylsilyl)acetamide gives an intermediate which is used in a Michael addition reaction or an Arbuzov reaction.
Complexation of lipids with cyclodextrin carriers for fully defined supplementation of cell culture (2021)

Induced Pluripotent Stem Cells (iPSCs) hold great promise for revolutionizing medicine and research. Scientists are currently able to reprogram adult cells of almost any type to a genetically 'open' state, pluripotency, wherein they lose the characteristics of their original cell type, and revert to a state that can reproduce indefinitely, and can be differentiated to many different cell types. IPSCs are currently grown in 'chemically defined' media that contains no animal derived components. This media eliminates animal and human sera because these tend to be quite variable and confound the reprogramming process, but the chemically defined media in use has almost no lipid content. The central goal of this project was to develop methods for chemically defined addition of lipids to cell culture media. The methods developed promise to be an advance in stem cell and general cell culture methodology, providing more reproducible experimental results, and supporting cells in culture with optimized lipid contents. In order to facilitate the addition of lipids to cell culture media without animal serum or serum albumin, complexation of individual lipids with a methyl beta-cyclodextrin starch was accomplished without addition of other molecules or oxidation of delicate lipids at a 1:1 stoichiometry. The lipid/MBCD complexes are soluble in aqueous media, and can be added individually or as a mixture to cell cultures. Application of complexed lipids to stem cells and fibroblasts revealed significant differences in lipid responses. Supplementation of human fibroblasts with a mixture of complexed lipids and other elements caused a 60% increase in proliferation over a 10 day period. Supplementation of stem cells with complexed lipids significantly increased proliferation, without reduction of pluripotency. Differences in lipid responses were also found between iPSC and embryonic stem cells, that may help elucidate differences between genetic or metabolic states which may point the way for more effective reprogramming of adult cells to pluripotency.
Nonlinear optical properties of organic crystals with hydrogen-bonded molecular units: The case of urea (1982)

The validity of simpler additive models, accounting for nonlinear optical properties of van der Waals crystals in terms of independent molecular contributions is questionable in the case of more associated molecular structures such as examplified by the hydrogen-bonded crystal lattice of urea. A number of one-electron properties of single isolated urea molecules are first considered by means of a finite-field perturbated LCAO Hartree–Fock model implemented at both semiempirical (INDO) and ab initio levels of approximation with different basis set extensions. Charge distribution, dipole (μ), multipoles, linear (α), quadratic (β), and cubic hyperpolarizabilities γ are computed and analyzed. The α tensor is shown to be quasi-isotropic in the molecular plane while the β tensor exhibits a stronger anisotropy and a prevailing βzzz coefficient (z is the molecular symmetry axis). The negative value of the product βzzzμz is interpreted by a decrease of the dominant excited state dipole as compared to that of the ground state dipole. A mutually consistent field approach coupled with a finite-field dipolar perturbation is developed at the LCAO Hartree–Fock ab initio level to account for the influence of the crystalline environment on molecular properties. Coulombian point-charge potentials are located around each molecule of the crystal, at specific atomic sites taking part in the lattice hydrogen bonding. The translational invariance of the crystalline charge distribution in the presence of the external field is ensured by a fast convergent iterative procedure. This model evidences, in the case of urea, a stronger anisotropic influence of the local crystalline environment on the lowest order nonlinear susceptibility (βzzz going from −44 to −74 a.u.) than on the polarizability (αzz, going from 27 to 31 a.u.). This noticeable enhancement of ‖βzzz‖ is linked to a possible cooperative influence of intramolecular charge-transfer and intermolecular bonding in the general case of a linear chain of hydrogen-bonded charge-transfer molecules interacting through the donor and acceptor groups.
A Role of Myosin Vb and Rab11‐FIP2 in the Aquaporin‐2 Shuttle (2006)

Arginine-vasopressin (AVP) regulates water reabsorption in renal collecting duct principal cells. Its binding to Gs-coupled vasopressin V2 receptors increases cyclic AMP (cAMP) and subsequently elicits the redistribution of the water channel aquaporin-2 (AQP2) from intracellular vesicles into the plasma membrane (AQP2 shuttle), thereby facilitating water reabsorption from primary urine. The AQP2 shuttle is a paradigm for cAMP-dependent exocytic processes. Using sections of rat kidney, the AQP2-expressing cell line CD8, and primary principal cells, we studied the role of the motor protein myosin Vb, its vesicular receptor Rab11, and the myosin Vb- and Rab11-binding protein Rab11-FIP2 in the AQP2 shuttle. Myosin Vb colocalized with AQP2 intracellularly in resting and at the plasma membrane in AVP-treated cells. Rab11 was found on AQP2-bearing vesicles. A dominant-negative myosin Vb tail construct and Rab11-FIP2 lacking the C2 domain (Rab11-FIP2-DeltaC2), which disrupt recycling, caused condensation of AQP2 in a Rab11-positive compartment and abolished the AQP2 shuttle. This effect was dependent on binding of myosin Vb tail and Rab11-FIP2-DeltaC2 to Rab11. In summary, we identified myosin Vb as a motor protein involved in AQP2 recycling and show that myosin Vb- and Rab11-FIP2-dependent recycling of AQP2 is an integral part of the AQP2 shuttle.