gebhart/patrepeval huggingface dataset:
Spectinomycin modification. II. Spectinomycin C-3'-modification via diazoketone intermediates. (1985)

The C-3'-carbonyl group of N-protected spectinomycin is efficiently converted into a diazo group via base treatment of the corresponding tosylhydrazone. The diazo group imparts a new synthetically useful reactivity pattern on the sugar ring of the molecule. The synthesis of C-3'-deoxo-, monohalo- and dihalospectinomycins via the intermediacy of these diazo compounds is described. The reduced bioactivity of these analogs as compared to the parent and the C-3'-dihydro and aminospectinomycins established the need for hydrogen bonding groups in this region of the molecule for good activity, further refining the structure activity relationships in the spectinomycin series.
ChemInform Abstract: Photochemical Synthesis of Disilane from Silane with Infrared Laser Radiation. (1989)

Abstract It is demonstrated that SiH 4 can be efficiently converted to Si 2 H 6 by CO 2 laser irradiation.
<title>Holographic anticounterfeit method and device with encoded pattern</title> (1999)

An advanced holographic anti-counterfeit method and device is proposed to make the labels, which are used to identify the commercial products, being highly difficult to duplicate. This method provides a dot-matrix hologram, which is formed with a synthesized image including a random dispersed background pattern and a creative graphic design as a hidden pattern encoded into the background pattern. The background pattern is visible to the naked eyes of a viewer, but the hidden pattern can be viewed only through a special viewing device, such as a lenticular lens sheet. If the lenticular lens sheet covers the hologram irrelevantly or with an unfitted specification, the hidden pattern can't be decoded, and the moire effect will appear. A logo of U.S. MAIL is demonstrated as the encoded pattern, and the synthesized image is recorded in a 35 mm X 35 nm dot- matrix hologram on the photo-plate.
High performance organic electroluminescent diodeswith microcavities (1996)

The authors have fabricated organic electroluminescent (EL) diodes with a microcavity structure designed to have a resonance wavelength of 500 nm, by using a metal reflector and various dielectric reflectors. The emission intensity (spectral power density) is enhanced by a factor of 5 compared to that of an EL diode without a cavity, and narrow spectral width of 8 nm was achieved in blue green emission.
Improved audio coding using a psychoacoustic model based on a cochlear filter bank (2002)

Perceptual audio coders use an estimated masked threshold for the determination of the maximum permissible just-inaudible noise level introduced by quantization. This estimate is derived from a psychoacoustic model mimicking the properties of. masking. Most psychoacoustic models for coding applications use a uniform (equal bandwidth) spectral decomposition as a first step to approximate the frequency selectivity of the human auditory system. However, the equal filter properties of the uniform subbands do not match the nonuniform characteristics of cochlear filters and reduce the precision of psychoacoustic modeling. Even so, uniform filter banks are applied because they are computationally efficient. This paper presents a psychoacoustic model based on an efficient nonuniform cochlear filter bank and a simple masked threshold estimation. The novel filter-bank structure employs cascaded low-order IIR filters and appropriate down-sampling to increase efficiency. The filter responses are optimized for the modeling of auditory masking effects. Results of the new psychoacoustic model applied to audio coding show better performance in terms of bit rate and/or quality of the new model in comparison with other state-of-the-art models using a uniform spectral decomposition. The low delay of the new model is particularly suitable for low-delay coders.
Abstract IA1: Building molecules to guide anticancer therapy (2011)

Abstract For clinical imaging, we need synthetic molecules with novel amplifying mechanisms for homing to diseased tissues. Activatable cell penetrating peptides (ACPPs) are polycationic cell penetrating peptides (CPPs) whose cellular uptake is minimized by a polyanionic inhibitory domain and then restored upon proteolysis of the peptide linker connecting the polyanionic and polycationic domains. Local activity of proteases able to cut the linker causes amplified retention in tissues and uptake into cells. ACPPs on dendrimers labeled with Cy5 and Gd-DOTA enable whole body magnetic resonance imaging (Olson et al (2010) PNAS 107: 4311–4316) followed by fluorescenceguided surgery. Such fluorescence guidance improves tumor-free survival in two animal models (Nguyen et al (2010) PNAS 107: 4317–4322). Contrast for tumor over normal tissues is amplified and accelerated when the polyanionic domain includes an acceptor of fluorescence resonance energy transfer (FRET), because loss of FRET (monitored either by multispectral emission or donor excited-state lifetime) instantly signals proteolysis without waiting for uncleaved substrate to wash out of normal tissues. Thrombin-cleavable ACPPs accumulate in atherosclerotic plaques, and their labeling intensity seems to correlate with progression towards rupture. Separately, we have developed fluorescent peptides that light up peripheral nerves to show surgeons where not to cut (Whitney et al (2011) Nature Biotech. 29: 352–356). Thus we believe that basic tumor biology and molecular design can help improve early detection and surgical resection, not just chemotherapy. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the Second AACR International Conference on Frontiers in Basic Cancer Research; 2011 Sep 14-18; San Francisco, CA. Philadelphia (PA): AACR; Cancer Res 2011;71(18 Suppl):Abstract nr IA1.
Comparison of the anti‐diabetic effects of GIP‐ and GLP‐1‐receptor activation in obese diabetic (<i>ob/ob</i>) mice: studies with DPP IV resistant <i>N</i>‐AcGIP and exendin(1–39)amide (2007)

The results illustrate significant anti-diabetic potential of N-AcGIP alone and in combination with exendin(1-39)amide.
Structure of AlAs-GaAs interfaces grown on (100) vicinal surfaces by molecular beam epitaxy (1984)

Hyperfine control of the interface structure and composition between GaAs and AlAs films grown by molecular beam epitaxy has been achieved by deposition on vicinal (100) GaAs substrates. This control is demonstrated by producing (GaAs)m-(AlAs)n submonolayer (m and/or n&lt;1) superlattices over a wide temperature range. Analysis of these submonolayer superlattices by transmission electron microscopy shows that the layer growth regime is dominant and that layer nucleation is initiated preferentially at the step edges on the (100) vicinal surface. Potential applications of submonolayer superlattices including the growth of superlattice layers perpendicular to the substrate surface are described.
Porcine Endogenous Retrovirus Transmission Characteristics of an Inbred Herd of Miniature Swine (2002)

Here we report the identification of inbred miniature swine that failed to produce human-tropic replication-competent porcine endogenous retroviruses (HTRC PERVs), using in vitro coculture assays. When HTRC PERVs were isolated from transmitting animals, all were recombinant viruses, with the receptor-binding domain of PERV-A combining with PERV-C-related sequences.
Estimation of Wideband IQ Imbalance in MIMO OFDM Systems With CFO (2021)

This paper deals with IQ imbalance in wideband MIMO OFDM systems. In systems with a large bandwidth, the IQ imbalance will be frequency-dependent and hence cannot be described by a single parameter. Furthermore if there is a carrier-frequency offset (CFO), the IQ imbalance estimation in a MIMO system becomes a challenging task. However the CFO introduces a time-varying component to the received signal which can be exploited to estimate the model parameters using a carefully designed training sequence. This approach obtains the TX and RX IQ imbalance separately in an accurate fashion. We verify this by simulations and also show the estimated IQ imbalance in a customised hardware prototype.
Phrase-Based Models (2009)

The currently best performing statistical machine translation systems are based on phrase-based models: models that translate small word sequences at a time. This chapter explains the basic principles of phrase-based models and how they are trained, and takes a more detailed look at extensions to the main components: the translation model and the reordering model. The next chapter will explain the algorithms that are used to translate sentences using these models.
Abnormal distribution of the non-Abeta component of Alzheimer's disease amyloid precursor/alpha-synuclein in Lewy body disease as revealed by proteinase K and formic acid pretreatment. (1998)

The precursor of the non-Abeta component of Alzheimer's disease amyloid (NACP) (also known as alpha-synuclein) is a presynaptic terminal molecule that abnormally accumulates in the plaques of Alzheimer's disease (AD) and in the Lewy bodies (LBs) of Lewy body variant of AD, diffuse Lewy body disease, and Parkinson's disease. To better understand the distribution of NACP/alpha-synuclein and its fragments in the LB-bearing neurons and neurites, as well as to clarify the patterns of NACP/alpha-synuclein compartmentalization, we studied NACP/alpha-synuclein immunoreactivity using antibodies against the C-terminal, N-terminal, and NAC regions after Proteinase K and formic acid treatment in the cortex of patients with LBs. Furthermore, studies of the subcellular localization of NACP/alpha-synuclein within LB-bearing neurons were performed by immunogold electron microscopy. These studies showed that the N-terminal antibody immunolabeled the LBs and dystrophic neurites with great intensity and, to a lesser extent, the synapses. In contrast, the C-terminal antibody strongly labeled the synapses and, to a lesser extent, the LBs and dystrophic neurites. Whereas Proteinase K treatment enhanced NACP/alpha-synuclein immunoreactivity with the C-terminal antibody, it diminished the N-terminal NACP/alpha-synuclein immunoreactivity. Furthermore, formic acid enhanced LB and dystrophic neurite labeling with both the C- and N-terminal antibodies. In addition, whereas without pretreatment only slight anti-NAC immunoreactivity was found in the LBs, formic acid pretreatment revealed an extensive anti-NAC immunostaining of LBs, plaques, and glial cells. Ultrastructural analysis revealed that NACP/alpha-synuclein immunoreactivity was diffusely distributed within the amorphous electrodense material in the LBs and as small clusters in the filaments of LBs and neurites. These results support the view that aggregated NACP/alpha-synuclein might play an important role in the pathogenesis of disorders associated with LBs.
A 28-GHz Quadrature Fractional-N Frequency Synthesizer for 5G Transceivers With Less Than 100-fs Jitter Based on Cascaded PLL Architecture (2017)

This paper introduces a quadrature fractional-N cascaded frequency synthesizer and its phase noise analysis, optimization, and design for future 5G wireless transceivers. The performance improvement of the cascaded phase-locked loop (PLL) over single-stage PLL in terms of jitter and power consumption is theoretically presented and verified with measured results. The cascaded PLL is implemented using a first-stage fractional-N charge-pump PLL followed by a second-stage quadrature dividerless subsampling PLL. The fractional division in the first-stage PLL is implemented using a high-resolution phase mixer for lower quantization noise. Two prototypes of the single-stage PLL and the cascaded PLL were implemented in the 65-nm bulk CMOS process. The 26-32 GHz quadrature cascaded PLL consumes a total of 26.9 mW from 1-V supply and achieves less than 100-fs integrated jitter with -116.2 and -112.6-dBc/Hz phase noise at 1-MHz offset for the integer-N and the fractional-N modes, respectively. The fractional-N single-stage and cascaded PLLs achieve figure-of-merits of -230.58 and -248.75 dB, respectively.
Myotilin, a novel sarcomeric protein with two Ig-like domains, is encoded by a candidate gene for limb-girdle muscular dystrophy (1999)

The striated muscle sarcomeres are highly organized structures composed of actin (thin) and myosin (thick) filaments that slide past each other during contraction. The integrity of sarcomeres is controlled by a set of structural proteins, among which are titin, a giant molecule that contains several immunoglobulin (Ig)-like domains and associates with thin and thick filaments, and [alpha]-actinin, an actin cross-linking protein. Mutations in several sarcomeric and sarcolemmal proteins have been shown to result in muscular dystrophy and cardiomyopathy. On the other hand, the disease genes underlying several disease forms remain to be identified. Here we describe a novel 57 kDa cytoskeletal protein, myotilin. Its N-terminal sequence is unique, but the C-terminal half contains two Ig-like domains homologous to titin. Myotilin is expressed in skeletal and cardiac muscle, it co-localizes with [alpha]-actinin in the sarcomeric I--bands and directly interacts with [alpha]-actinin. The human myotilin gene maps to chromosome 5q31 between markers AFM350yB1 and D5S500. The locus of a dominantly inherited limb-girdle muscular dystrophy (LGMD1A) resides in an overlapping narrow segment, and a new type of distal myopathy with vocal cord and pharyngeal weakness (VCPMD) has been mapped to the same locus. The muscle specificity and apparent role as a sarcomeric structural protein raise the possibility that defects in the myotilin gene may cause muscular dystrophy.
Treating cardiac disease with catheter-based tissue heating (2002)

Two applications of microwave internal biological heating are discussed. Both microwave assisted balloon angioplasty (MABA) and microwave cardiac ablation (MCA) consist of an antenna applicator fed by means of coaxial cable, which passes through a catheter. The antenna designs take advantage of polarization and phase effects of microwaves to create specific power deposition patterns. MABA with a helix and mode filter balloon uses the large differences in the dielectric characteristics of high water content (HWC) and low water content (LWC) tissue to preferentially heat and weld plaque while sparing healthy artery walls. Wide aperture MCA uses an unfurlable spiral antenna within a balloon to generate a deep large ablation volume in diseased cardiac tissue. Theoretical studies have been validated with a variety of in-vitro and in-vivo experiments. There is less of a potential for tissue-surface charring with microwaves than with RF ablation. Live animal studies indicate that MCA is well tolerated by animals.
Further Investigation of Effects of Injection Pressure and Imbibition Water on CO<sub>2</sub> Huff-n-Puff Performance in Liquid-Rich Shale Reservoirs (2018)

Shale oil production has increased rapidly in the past decades, especially in the United States, and results in a revolution in the energy landscape. However, one main problem existing in the shale reservoir development is the sharp decline of liquids production in all the hydraulically fractured wells. In recent years, CO2 huff-n-puff injection has been proved to be a potential method to enhance the oil recovery. In this study, the effects of injection pressure and imbibition water on CO2 huff-n-puff performance were further investigated. Eagle Ford core samples and Wolfcamp dead oil were used in this experimental study. The microscopic pore characteristics of Eagle Ford shale core samples were analyzed, and the results show that 98.08% of the pore sizes are distributed between 3 nm and 50 nm. The experimental results demonstrate the great potential of CO2 huff-n-puff EOR. The cumulative oil recovery can reach 68% after seven huff-n-puff cycles. The oil recovered in each cycle deceases as injection cycle number increases due to the permeability damage caused by asphaltene precipitation, oil saturation reduction, and low injected CO2 sweep efficiency. The effect of injection pressure was studied by injecting CO2 at both immiscible and miscible conditions. CO2 huff-n-puff has better performance (more than 9.1% EOR) under miscible conditions than immiscible conditions. After that, a novel experiment was designed to saturate the core samples with both water (15 wt % KCl) and Wolfcamp dead oil to investigate the influence of imbibition water on CO2 huff-n-puff EOR performance. The existence of imbibition water impedes oil production in shale core samples. The oil recovery decreased about 45.3% after seven huff-n-puff cycles compared to the condition without water. A simulation study provides a better understanding of CO2 huff-n-puff application in liquid-rich shale reservoirs, which is fundamentally important for applying and optimizing CO2 huff-n-puff in field production.
Chromatographic Preparation of a Therapeutic Highly Purified von Willebrand Factor Concentrate from Human Cryoprecipitate (1992)

A therapeutic highly purified von Willebrand factor (vWF) concentrate has been prepared from cryoprecipitate by a three-step chromatographic procedure. After solvent/detergent treatment to inactivate viruses, the cryoprecipitate solution was chromatographed on DEAE-fractogel TSK 650 M to separate vWF from most cryoprecipitate proteins, including factor VIII (FVIII) and fibrinogen. A second DEAE-fractogel TSK 650 M was then performed to further purify vWF and to allow concentrating it to over 100 U ristocetin cofactor activity/ml. The last step on immobilized gelatin removed fibronectin and increased the purity of vWF. vWF was recovered with about 18 and 40% yield in antigen and collagen-binding (CB) activity, respectively, from cryoprecipitate. vWF was obtained in an essentially pure state corresponding to a purification factor of over 10,000-fold from plasma. Immunonephelometric and SDS-PAGE analyses of the concentrate did not reveal any detectable cryoprotein contaminants, especially fibrinogen, fibronectin, immunoglobulins and albumin. The content in intermediate- and high-molecular-weight multimers in the concentrate was similar or higher than that of plasma, as the ion-exchanger selectively favored the binding and concentration of the larger multimeric forms while reducing the amount of the smaller forms with abnormal structure and low activity. Other characteristics of the concentrate included a CB activity to antigen ratio of 1.69 and a high capacity (86%) to correct platelet adhesion in a perfusion system. Clinical use of this standardized vWF concentrate has been shown to be efficacious in the treatment of vWF patients.
Gain interaction in the design of bend-resistant large mode area amplifier fibers (2007)

Fibers with very large mode area see degraded gain competition when bent for spooled-fiber operation. Simulations demonstrate that recently proposed bend-distortion-resistant designs are immune to gain degradation, as well as effective area reduction.
Chemokines in the pathogenesis of liver disease: so many players with poorly defined roles (2002)

Many new chemokines have been described in recent years, resulting in a new classification of these chemoattractant proteins. The characterization of the biological functions of most chemokines relates to their ability to induce chemotaxis in circulating inflammatory cells. However, it is now clear that chemokines have a much wider biological role, including angiogenesis, carcinogenesis and involvement in the pathogenesis of HIV infection. Our understanding of the role of chemokines in the pathogenesis of disorders of the lungs and brain outstrips that with regard to disorders of the liver. An increased understanding of the role of chemokines in the pathogenesis of liver disease may lead to the development of novel therapies for hepatic disease.
Quality Assurance in Gynecologic Cytology (2001)

We describe a simple method for displaying and evaluating the concordance or discordance between cytotechnologists (CTs) and cytopathologists (CPs) on gynecologic cases. The provisional diagnoses made by the CTs and the final diagnoses of the CPs are captured by the laboratory information system; data generated for specified periods are displayed as a 10 x 10 matrix that classifies each possible diagnosis made by the CT and CP into 1 of 10 major categories. Matrices are generated for the entire laboratory and for individual CTs; individual CTs are evaluated based on their deviation from the laboratory average. Three statistical measures are generated: percentage of discordant diagnoses, a kappa statistic, and a weighted measure. During a 2.5-year period, approximately 4,200 cases were referred to a CP for review every 6 months. The median discordance in diagnoses increased during 2 years from 21% to 34%, and the kappa value fell from 0.69 to 0.38. This was attributed primarily to 1 CT, whose performance, as well as that of the entire laboratory, improved after remedial action. Measures of CT-CP diagnostic concordance are a useful and efficient measure of CT performance and can be incorporated into mandatory semiannual performance evaluations.
Self‐Assembled SERS Substrates with Tunable Surface Plasmon Resonances (2011)

Abstract The fabrication of surface‐enhanced Raman spectroscopy (SERS) substrates that are optimized for use with specific laser wavelength–analyte combinations is addressed. In order to achieve large signal enhancement, temporal stability, and reproducibility over large substrate areas at low cost, only self‐assembly and templating processes are employed. The resulting substrates consist of arrays of gold nanospheres with controlled diameter and spacing, properties that dictate the optical response of the structure. Tunability of the extended surface plasmon resonance is observed in the range of 520–1000 nm. It is demonstrated that the enhancement factor is maximized when the surface plasmon resonance is red‐shifted with respect to the SERS instrument laser line. Despite relying on self‐organization, site‐to‐site enhancement factor variations smaller than 10% are obtained.
The principle of delivery of T cell epitopes to antigen-presenting cells applied to peptides from influenza virus, ovalbumin, and hen egg lysozyme: Implications for peptide vaccination (2001)

Targeting of antigens to antigen-presenting cells (APCs) increases CD4(+) T cell activation, and this observation can be exploited in the development of new vaccines. We have chosen an antigen-targeting approach in which we make recombinant antibodies (Abs) with T cell epitopes in their constant region and APC-specific variable regions. Three commonly used model epitopes, amino acids 110-120 of hemagglutinin, 323-339 of ovalbumin, and 46-61 of hen egg lysozyme, were introduced as loops in the C(H)1 domain of human IgG3. For all three epitopes, we show that the recombinant molecules are secreted from transfected cells. The epitopes are presented to specific T cells, and targeting to IgD on B cells in vitro enhances the presentation efficiency by 10(4) to 10(5) compared with the free peptide. After i.v. injection, the epitopes targeted to IgD are presented by splenic APCs to activate specific T cells, whereas little or no activation could be detected without targeting, even after the amount of antigen injected was increased 100-fold or more. Because a wide variety of T cell epitopes, in terms of both length and secondary structure, can be tolerated in loops in constant domains of Abs, the Ab constant region seems to have the intrinsic stability that is needed for this fusion molecule strategy. It might thus be possible to load the Ab with several different epitopes in loops in different domains and thereby make a targeted multisubunit vaccine.