gebhart/patrepeval huggingface dataset:
Autologous blood coagulum is a physiological carrier for BMP6 to induce new bone formation and promote posterolateral lumbar spine fusion in rabbits (2019)

In the present study, we describe autologous blood coagulum (ABC) as a physiological carrier for BMP6 to induce new bone formation. Recombinant human BMP6 (rhBMP6), dispersed within ABC and formed as an autologous bone graft substitute (ABGS), was evaluated either with or without allograft bone particles (ALLO) in rat subcutaneous implants and in a posterolateral lumbar fusion (PLF) model in rabbits. ABGS induced endochondral bone differentiation in rat subcutaneous implants. Coating ALLO by ABC significantly decreased the formation of multinucleated foreign body giant cells (FBGCs) in implants, as compared with ALLO alone. However, addition of rhBMP6 to ABC/ALLO induced a robust endochondral bone formation with little or no FBGCs in the implant. In rabbit PLF model, ABGS induced new bone formation uniformly within the implant resulting in a complete fusion when placed between two lumbar transverse processes in the posterolateral gutter with an optimum dose of 100-μg rhBMP6 per ml of ABC. ABGS containing ALLO also resulted in a fusion where the ALLO was replaced by the newly formed bone via creeping substitution. Our findings demonstrate for the first time that rhBMP6, with ABC as a carrier, induced a robust bone formation with a complete spinal fusion in a rabbit PLF model. RhBMP6 was effective at low doses with ABC serving as a physiological substratum providing a permissive environment by protecting against foreign body reaction elicited by ALLO.
Sphingosine Kinase-Dependent Migration of Immature Dendritic Cells in Response to Neurotoxic Prion Protein Fragment (2003)

The concept that circulating dendritic cells mediate neuroinvasion in transmissible spongiform encephalopathies received strong support from recent observations that prion protein is expressed in myeloid dendritic cells. We observed that prion protein fragment 106-126 is a chemoattractant for monocyte-derived immature but not mature dendritic cells. Signaling events in chemotaxis involved enzymes downstream of G(q) protein and were inhibited by blockade of sphingosine kinase, suggesting transactivation of sphingosine-1-phosphate-dependent cell motility by prion protein.
Bioterrorism, Glanders and melioidosis (2005)

We note with interest the recently published guidelines for management of melioidosis and glanders. We are clinicians with extensive experience with melioidosis in Australia and Thailand and would like to express our concern at a number of inaccuracies in these guidelines.
Wearable lifestyle tracking devices (2015)

Behavioral patterns linked to physical activity and nutrition are established during adolescence and people tend to maintain them throughout their whole lives. Wearable life tracking devices might be a useful tool in order to achieve healthy lifestyles among teens. The objective of this study is to investigate what teens think of current wearable devices for fitness tracking, using the results to provide preliminary suggestions for the design of wearable technology aimed at adolescents. Thirty 14--16 teens (sex balanced) divided into 3 groups were asked to trial use commercially available wearables for one week. Each group took part in two focus group sessions, separated by a week. During the trial users experienced some technical problems while using the devices. Monitoring physical activity seems not to be a priority for adolescents but they recognize the important potential in doing it. Recommendations were suggested for the devices to better meet teenage preferences and needs.
HTLV-III <i>gag</i> Protein Is Processed in Yeast Cells by the Virus <i>pol</i> -Protease (1986)

The gag-pol gene of HTLV-III (human T-lymphotropic virus), the virus linked to AIDS (acquired immune deficiency syndrome), was expressed in yeast, and processing of the gag precursor into proteins of the same size as those in the virion was observed. Processing of the gag gene in yeast cells mimics the process that naturally occurs in mammalian cells during maturation of virions. Therefore it was possible to perform mutational analysis of the virus genome to localize the gene that codes for the protease function to the amino terminal coding region of the pol gene. Since this region overlaps the gag gene, it is likely that ribosomal frameshifting occurs from gag to pol. Antibodies in all of the AIDS patients' sera tested recognized the yeast synthesized gag proteins, although the sera showed differences in relative reactivity to the individual gag proteins and the precursor. This yeast system should be valuable not only for production of viral proteins for diagnostic or vaccine purposes but also for analysis of the genetics and biochemistry of viral gene functions--parameters that are difficult to study otherwise with this virus.
Stability considerations of permanent magnet quadrupoles for CESR phase-III upgrade (1998)

The Cornell electron storage ring (CESR) phase-III upgrade plan includes very strong permanent magnet quadrupoles in front of the cryostat for the superconducting quadrupoles and physically as close as possible to the interaction point. Together with the superconducting quadrupoles, they provide tighter vertical focusing at the interaction point. The quadrupoles are built with neodymium iron boron (NdFeB) material and operate inside the 15 kG solenoid field. Requirements on the field quality and stability of these quadrupoles are discussed and test results are presented.
Transmission line aspects of the design of broad-band electrooptic traveling-wave modulators (1987)

This paper proposes a two-layer traveling-wave type electrooptic modulator structure to maximize modulation bandwidth from transmission line aspects. This structure can be designed to satisfy the velocity matching and impedance matching condition simultaneously. A design method for this structure is discussed.
Effect of follicular steroids on the maturation and fertilization of mammalian oocytes (1980)

Pronuclear development was used to measure the effects on ovine oocytes of altering follicular steroidogenesis during maturation in vitro. Follicular steroid secretion was altered using enzyme inhibitors and exogenous steroid supplementation. Abnormalities induced during maturation were measured 24 h after tranfer of oocytes to the oviducts of inseminated hosts. The presence throughout maturation of aminoglutethimide, an inhibitor of the conversion of cholesterol to pregnenolone, reduced steroid secretion to 7% of that in controls and decreased from 77% to 33% the number of normal oocytes. Abnormalities were substantially reduced by the addition of aminoglutethimide during the final 8 h of maturation only. The inhibition of 17 alpha-hydroxylase enzymes with SU10603 reduced oestrogen and testosterone secretion to about 10% of control levels but had no effect on progestin secretion. Only 13% of oocytes matured in the continual presence of SU10603 underwent normal fertilization. The number of oocytes undergoing normal fertilization was increased to about 50% by (i) delaying the addition of SU10603 until the last 8 h of the maturation period or (ii) adding exogenous steroids to follicles cultured with inhibitor from explantation. It is concluded that oocytes require a specific intra-follicular steroid environment for the completion of the full maturation process. Alterations to the steroid profile during maturation induce changes in the oocyte which are expressed as gross abnormalities at fertilization.
Immune checkpoint blockade for hematologic malignancies: a review (2017)

Immune checkpoint blockade has revolutionized the treatment of cancer, with impressive responses seen in a broad variety of tumor types. Blockade of immune checkpoints and immune signaling antibodies has shown promise in multiple types of hematologic malignancies (HMs), with dramatic single agent responses for pembrolizumab and nivolumab in Hodgkin lymphoma (HL). In this review, we outline the current state of immune checkpoint blockade drug development in HMs, and discuss mechanisms of activity and resistance, and highlight potential targets in the immune tumor microenvironment (TME). Blockade of T-cell checkpoint molecules PD-1/PD-L1 and CTLA-4 are the most clinically mature of the immune checkpoint strategies. Novel and upcoming strategies for immune checkpoint blockade drug development in HMs using innovative combinations to modulate immunologic targets shows significant promise as a way to expand the number of patients with blood cancers who could benefit from immunotherapy.
Mediated tabletop interaction in the biology lab (2011)

Interactive surfaces like diffuse illumination tabletops (DIT) identify and track objects using multiple techniques like shape and color recognition, fiducial markers, electronic components, and RFID tags. However, tracking becomes more complex when dealing with multiple small objects of similar form. We propose to use tangible mediators for integrating such objects to tabletops. This paper reports on our initial explorations of mediated tabletop interaction consisting of a mediator prototype and a design space definition. We built a mediator, the Rabbit, a device that translates thevalue of an RFID tag into a visual 2D code. The Rabbit rests on the interactive surface, holds the object, reads its passive RFID tag, and converts the read value into a 2D code that can be read by the DIT's built-in camera. When handling multiple objects, the Rabbit iterates through the generated 2D codes. Through a series of participatory activities with end users (molecular biologists), we collected initial feedback from participants and defined a design space for mediated tabletop interaction.
Three-Dimensional Human Skin Equivalent as a Tool To Study Acinetobacter baumannii Colonization (2012)

Acinetobacter baumannii can colonize body surfaces of hospitalized patients. From these sites, invasion into the host and spread to other patients and the hospital environment may occur. The eradication of the organism from the patient's skin is an important infection control strategy during epidemic and endemic episodes. In this study, a three-dimensional (3D), air-exposed human epidermal skin equivalent was exploited to study Acinetobacter skin colonization. We characterized the adherence of A. baumannii ATCC 19606(T) and Acinetobacter junii RUH2228(T) to and biofilm formation on the skin equivalent and the responses to these bacteria. Furthermore, we assessed the ability of the disinfectant chlorhexidine to decolonize the skin equivalents. The results revealed that both strains replicated on the stratum corneum for up to 72 h but did not invade the epidermis. A. baumannii, in contrast to A. junii, formed large biofilms on the stratum corneum. Bacterial colonization did not affect keratinocyte activation, proliferation, or differentiation, nor did it induce a strong inflammatory response. Disinfection with chlorhexidine solution resulted in complete eradication of A. baumannii from the skin, without detrimental effects. This 3D model is a promising tool to study skin colonization and to evaluate the effects of novel disinfectant and antimicrobial strategies.
On-Chip processing for the wave union TDC implemented in FPGA (2009)

The wave union TDC implemented in FPGA utilizes multiple measurement method to reach time resolution beyond the natural carry cell delay in FPGA. Lacking of analog compensation for bin width control available in ASIC, the wave union TDC takes the after-fact digital calibration approach. In addition to the temperature drift, non-uniformity of the carry chain structure in FPGA causes complicate differential non-linearity pattern which imposes significant on-chip calibration challenge. In this paper, processing strategies for the wave union TDC are discussed. Actual implementations in low-cost FPGA with 20 ps and 10 ps RMS resolutions are also presented.
A Survey of Schema Matching Research (2007)

A Survey of Schema Matching Research Schema matching is the process of developing semantic matches between two or more schemas. The purpose of schema matching is generally either to merge two or more databases, or to enable queries on multiple, heterogeneous databases to be formulated on a single schema (Doan and Halevy 2005). This paper develops a taxonomy of schema matching approaches, classifying them as being based on a combination schema matching technique and the type of data used by those techniques. Schema matching techniques are categorized as being based on rules, learning, or ontology, and the type of data used is categorized as being based on schema elements or instance data. This taxonomy is an extension to previous work, and significant current research efforts are categorized using this taxonomy. Several of these research efforts are profiled and their categorization in the taxonomy is explored. The current research is used to identify the directions in which future research is headed.
Assessment of <i>Helicobacter pylori</i> Gene Expression within Mouse and Human Gastric Mucosae by Real-Time Reverse Transcriptase PCR (2001)

Despite increasing knowledge on the biology of Helicobacter pylori, little is known about the expression pattern of its genome during infection. While mouse models of infection have been widely used for the screening of protective antigens, the reliability of the mouse model for gene expression analysis has not been assessed. In an attempt to address this question, we have developed a quantitative reverse transcriptase PCR (RT-PCR) that allowed the detection of minute amounts of mRNA within the gastric mucosa. The expression of four genes, 16S rRNA, ureA (encoding urease A subunit), katA (catalase), and alpA (an adhesin), was monitored during the course of a 6-month infection of mice and in biopsy samples from of 15 infected humans. We found that the selected genes were all expressed within both mouse and human infected mucosae. Moreover, the relative abundance of transcripts was the same (16S rRNA > ureA > katA > alpA), in the two models. Finally, results obtained with the mouse model suggest a negative effect of bacterial burden on the number of transcripts of each gene expressed per CFU (P < 0.05 for 16S rRNA, alpA, and katA). Overall, this study demonstrates that real-time RT-PCR is a powerful tool for the detection and quantification of H. pylori gene expression within the gastric mucosa and strongly indicates that mice experimentally infected with H. pylori provide a valuable model for the analysis of bacterial gene expression during infection.
Influence of Rock Inhomogeneity on the Dynamic Tensile Strength of Rock (2003)

The fracture processes under dynamic loading in tension were simulated using a proposed numerical approach and analyzed to determine dynamic tensile strength. The dynamic tensile strength and the scatter of the strength data decreased with increasing uniformity coefficients. The differences of static and dynamic tensile strength were due to the stress concentrations and redistribution mechanisms in the rock specimen. Although there were different mechanisms for the static and dynamic fracture processes, the static and dynamic tensile strengths were close to the mean microscopic tensile strength at high values of the uniformity coefficient. This paper shows that the rock inhomogeneity has an effect on dynamic tensile strength and is a factor that contributes to the different specimen strengths under dynamic and static loading conditions.
THE ANTIMICROBIAL ACTIVITIES OF CYCLOSPORINE, FK506, AND RAPAMYCIN (1994)

Transplantation is published monthly and is the most cited and influential journal in the field, with more than 25,000 citations per year. The journal celebrated its 50th year in 2016. Transplantation has been the trusted source for extensive and timely coverage of the most important advances in transplantation. The Editors and Editorial Board are an international group of research and clinical leaders that includes many pioneers of the field, representing a diverse range of areas of expertise. This capable editorial team provides thoughtful and thorough peer review, and delivers rapid, careful and insightful editorial evaluation of all manuscripts submitted to the journal.
Electrothermally tunable MEMS filters (2014)

MEMS resonators have potential application in the area of frequency selective devices (e.g., gyroscopes, mass sensors, etc.). In this paper, design of electro thermally tunable resonators is presented. SOIMUMPs process is used to fabricate resonators with springs (beams) and a central mass. When voltage is applied, due to joule heating, temperature of the conducting beams goes up. This results in increase of electrical resistance due to mobility degradation. Due to increase in the temperature, springs start softening and therefore the fundamental frequency decreases. So for a given structure, one can modify the original fundamental frequency by changing the applied voltage. Coupled thermal effects result in non-uniform heating. It is observed from measurements and simulations that some parts of the beam become very hot and therefore soften more. Consequently, at higher voltages, the structure (equivalent to a single resonator) behaves like coupled resonators and exhibits peak splitting. In this mode, the given resonator can be used as a band rejection filter. This process is reversible and repeatable. For the designed structure, it is experimentally shown that by varying the voltage from 1 to 16V, the resonant frequency could be changed by 28%.
Dyspareunia in Postmenopausal Women: A Critical Review (2008)

Postmenopausal dyspareunia occurring concurrently with vaginal atrophy is strongly associated with a lack of estrogen in the genital tract. However, a significant percentage of postmenopausal women experience dyspareunic pain that is not caused by hypoestrogenism. It is likely that other types of dyspareunia that occur premenopausally are also occurring in postmenopausal women. Research is needed to adequately address this issue. A change in perspective toward a multiaxial pain-focused approach is proposed for future research concerning dyspareunia in postmenopausal women.
MiTF links Erk1/2 kinase and p21CIP1/WAF1 activation after UVC radiation in normal human melanocytes and melanoma cells (2010)

As a survival factor for melanocytes lineage cells, MiTF plays multiple roles in development and melanomagenesis. What role MiTF plays in the DNA damage response is currently unknown. In this report we observed that MiTF was phosphorylated at serine 73 after UVC radiation, which was followed by proteasome-mediated degradation. Unlike after c-Kit stimulation, inhibiting p90RSK-1 did not abolish the band shift of MiTF protein, nor did it abolish the UVC-mediated MiTF degradation, suggesting that phosphorylation on serine 73 by Erk1/2 is a key event after UVC. Furthermore, the MiTF-S73A mutant (Serine 73 changed to Alanine via site-directed mutagenesis) was unable to degrade and was continuously expressed after UVC exposure. Compared to A375 melanoma cells expressing wild-type MiTF (MiTF-WT), cells expressing MiTF-S73A mutant showed less p21 WAF1/CIP1 accumulation and a delayed p21 WAF1/CIP1 recovery after UVC. Consequently, cells expressing MiTF-WT showed a temporary G1 arrest after UVC, but cells expressing MiTF-S73A mutant or lack of MiTF expression did not. Finally, cell lines with high levels of MiTF expression showed higher resistance to UVC-induced cell death than those with low-level MiTF. These data suggest that MiTF mediates a survival signal linking Erk1/2 activation and p21 WAF1/CIP1 regulation via phosphorylation on serine 73, which facilitates cell cycle arrest. In addition, our data also showed that exposure to different wavelengths of UV light elicited different signal pathways involving MiTF.
Development of a Walking Assist Machine Supporting Whole Legs from Their Soles (2006)

This paper proposes a walking assist machine for the elderly or rehabilitants, which uses a spatial parallel link mechanism. Its flat steps move in parallel with the ground and can support the whole legs of a person including his/her soles. A prototype was developed, which was designed to assist the halfpower of walking. Its performance was evaluated by experimental load tests corresponding to the half weight of each leg. Furthermore, to confirm the validity of our supporting method, muscle activity of the leg while wearing the walking assist machine was evaluated by using the surface electromyography (EMG) and calculating the ratio of the integrated EMG (IEMG) with and without the walking assist machine. The experimental results show that the activities of the rectus femoris muscle and the tibialis anterior muscle were particularly decreased to approximately sixty percent by wearing the walking assist machine.
HSP72 Inhibits Smad3 Activation and Nuclear Translocation in Renal Epithelial-to-Mesenchymal Transition (2010)

Although heat shock protein 72 (HSP72) ameliorates renal tubulointerstitial fibrosis by inhibiting epithelial-to-mesenchymal transition (EMT), the underlying mechanism is unknown. Because Smad proteins transduce TGF-beta signaling from the cytosol to the nucleus and HSP72 assists in protein folding and facilitates nuclear translocation, we investigated whether HSP72 inhibits TGF-beta-induced EMT by modulating Smad expression, activation, and nuclear translocation. To evaluate the roles of distinct HSP72 structural domains in these processes, we constructed vectors that expressed wild-type HSP72 or mutants lacking either the peptide-binding domain (HSP72-DeltaPBD), which is responsible for substrate binding and refolding, or the nuclear localization signal (HSP72-DeltaNLS). Overexpression of wild-type HSP72 or HSP72-DeltaNLS inhibited TGF-beta1-induced EMT, but HSP72-DeltaPBD did not, suggesting a critical role for the PBD in this inhibition. HSP72 overexpression inhibited TGF-beta1-induced phosphorylation and nuclear translocation of Smad3 and p-Smad3, but not Smad2; these inhibitory effects required the PBD but not the NLS. Coimmunoprecipitation assays suggested a physical interaction between Smad3 and the PBD. siRNA knockdown of endogenous HSP72 enhanced both TGF-beta1-induced Smad3 phosphorylation and EMT and confirmed the interaction of HSP72 with both Smad3 and p-Smad3. In vivo, induction of HSP72 by geranylgeranylacetone suppressed Smad3 phosphorylation in renal tubular cells after unilateral ureteral obstruction. In conclusion, HSP72 inhibits EMT in renal epithelial cells primarily by exerting domain-specific effects on Smad3 activation and nuclear translocation.
The personal satellite assistant: an internal spacecraft autonomous mobile monitor (2004)

This paper presents an overview of the research and development effort at the NASA Ames Research Center to create an internal spacecraft autonomous mobile monitor capable of performing intra-vehicular sensing activities by autonomously navigating onboard the International Space Station. We describe the capabilities, mission roles, rationale, high-level functional requirements, and design challenges for an autonomous mobile monitor. The rapid prototyping design methodology used, in which five prototypes of increasing fidelity are designed, is described as well as the status of these prototypes, of which two are operational and being tested, and one is actively being designed. The physical test facilities used to perform ground testing are briefly described, including a micro-gravity test facility that permits a prototype to propel itself in 3 dimensions with 6 degrees-of-freedom as if it were in a micro-gravity environment. We also describe an overview of the autonomy framework and its components including the software simulators used in the development process. Sample mission test scenarios are also described. The paper concludes with a discussion of future and related work followed by the summary.