gebhart/patrepeval huggingface dataset:
Data hiding for halftone images (2000)

With the ease of distribution of digital images, there is a growing concern for copyright control and authentication. While there are many existing watermarking and data hiding methods for natural images, almost none can be applied to halftone images. In this paper, we proposed two novel data hiding methods for halftone images. The proposed Data Hiding Pair-Toggling (DHPT) hides data by forced complementary toggling at pseudo-random locations within a halftone image. It is found to be very effective for halftone images with relatively coarse textures. For halftone images with fine textures (such as error diffusion with Steinberg kernel), the proposed Data Hiding Error Diffusion (DHED) gives significantly better visual quality by integrating the data hiding into the error diffusion operation. Both DHPT and DHED are computationally very simple and yet effective in hiding a relatively large amount of data. Both algorithms yield halftone images with good visual quality.
Mutation at a Single Position in the V2 Domain of the HIV-1 Envelope Protein Confers Neutralization Sensitivity to a Highly Neutralization-Resistant Virus (2010)

Understanding the determinants of neutralization sensitivity and resistance is important for the development of an effective human immunodeficiency virus type 1 (HIV-1) vaccine. In these studies, we have made use of the swarm of closely related envelope protein variants (quasispecies) from an extremely neutralization-resistant clinical isolate in order to identify mutations that conferred neutralization sensitivity to antibodies in sera from HIV-1-infected individuals. Here, we describe a virus with a rare mutation at position 179 in the V2 domain of gp120, where replacement of aspartic acid (D) by asparagine (N) converts a virus that is highly resistant to neutralization by multiple polyclonal and monoclonal antibodies, as well as antiviral entry inhibitors, to one that is sensitive to neutralization. Although the V2 domain sequence is highly variable, D at position 179 is highly conserved in HIV-1 and simian immunodeficiency virus (SIV) and is located within the LDI/V recognition motif of the recently described α4β7 receptor binding site. Our results suggest that the D179N mutation induces a conformational change that exposes epitopes in both the gp120 and the gp41 portions of the envelope protein, such as the CD4 binding site and the MPER, that are normally concealed by conformational masking. Our results suggest that D179 plays a central role in maintaining the conformation and infectivity of HIV-1 as well as mediating binding to α4β7.
Fast Elliptic Curve Arithmetic and Improved Weil Pairing Evaluation (2002)

We present an algorithm which speeds scalar multiplication on a general elliptic curve by an estimated 3.8 % to 8.5 % over the best known general methods when using affine coordinates. This is achieved by eliminating a field multiplication when we compute 2P+Q from given points P, Q on the curve. We give applications to simultaneous multiple scalar multiplication and to the Elliptic Curve Method of factorization. We show how this improvement together with another idea can speed the computation of the Weil and Tate pairings by up to 7.8 %.
ChemInform Abstract: The Zyggregator Method for Predicting Protein Aggregation Propensities (2008)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Tolerance Induction in the Adult Using Monoclonal Antibodies to CD4, CD8, and CD11a (LFA-1) (1989)

A major challenge in immunology has been to find a means of selectively abolishing an individual's potential to mount an immune response to certain antigens while preserving responsiveness to others. If this were possible, there would be major therapeutic implications for organ and marrow transplantation, the control of allergy, and the treatment of autoimmune diseases. The fact that transplantation tolerance could be achieved by injection of foreign donor hematopoietic cells into the neonatal mouse (Billingham et al. 1953) suggested that the same might be possible in the adult animal if a comparable state of immunological naiveté could be recreated. An array of different immunosuppressive regimes that destroyed lymphocytes (e.g., irradiation, antilymphocyte globulin, and thoracic duct drainage) could all facilitate tolerance induction (for review, see Weigle 1973) in mature animals. With the development of monoclonal antibodies to cells of the immune system, a new means of producing immunosuppression was possible. Cobbold...
Two Classes of Highly Similar Coiled Coil-Nucleotide Binding-Leucine Rich Repeat Genes Isolated from the <i>Rps1</i>-k Locus Encode <i>Phytophthora</i> Resistance in Soybean (2005)

A series of single genes protect soybean from the root and stem disease caused by the oomycete pathogen Phytophthora sojae. In the last two decades, Rps1-k has been the most stable and widely used Phytophthora resistance gene for the major soybean-producing regions of the United States. Four highly similar genes encoding coiled coil-nucleotide binding-leucine rich repeat (CC-NB-LRR)-type proteins were isolated from the Rps1-k locus. These genes were grouped into two classes based on their sequence identity. Class I contains three genes with identical open reading frames (ORF) and 5' end regions. Two of these genes were also identical at the 3' untranslated regions; the third gene showed a recombination breakpoint in the 3' untranslated region resulting in the combination of 3' end sequences of members from both classes. Reverse transcription-polymerase chain reaction analyses suggested that members of both classes of genes are transcribed at low levels. Representative members from each gene class were expressed in transgenic soybean plants. Analyses of independent R0, R1, R2, and R3 progeny populations suggested that both gene classes confer Phytophthora resistance in soybean. A possible evolutionary mechanism for the Class I gene family is proposed.
Recent advances in the development of a multiwavelength imaging pyrometer (1997)

A multiwavelength imaging pyrometer (MWIP) is described that permits real-time remote sensing of temperature profiles of targets with unknown emissivity by measuring the spectral radiance of a target at several distinct wavelengths using a 3203122-element PtSi ir CCD imager with an assembly of seven narrowband ir filters in the range from 1790 to 4536 nm. Based on these measurements, the temperature and model parameters of the target emissivity are determined simultaneously from the least-squares fit of the theoretical model of the ir camera output signal to the experimental data. The real-time least-squares minimization is accomplished by combination of Levenberg-Marquardt and simulatedannealing algorithms. The experimental MWIP system also includes a least-squares-based calibration algorithm for evaluation of effective values of peak filter transmissions and center wavelengths based on the detection of radiation emitted by the precalibrated blackbody source over a wide range of temperatures. To achieve high radiometric accuracy, the ir CCD camera was operated with black-level and background subtraction and with compensation for dark-current charge as a function of the detected signal level. To minimize the effect of the response nonlinearity on the accuracy of real-time MWIP temperature estimation, we have developed an algorithm that provides for imager operation at fixed preselected signal level for each spectral channel by adaptively changing the duration of the optical integration time of the imager. Initial testing demonstrated an accuracy of ± 1.0°C for real-time temperature measurements of the center of the blackbody aperture in the range from 500 to 1000°C. Temperature resolution of ± 3°C was demonstrated for the blackbody source viewed through a double-side polished silicon wafer with unknown spectral transmissivity in the temperature range from 500 to 900°C.
Energy-efficient polymeric gas separation membranes for a sustainable future: A review (2013)

Over the past three decades, polymeric gas separation membranes have become widely used for a variety of industrial gas separations applications. This review presents the fundamental scientific principles underpinning the operation of polymers for gas separations, including the solution-diffusion model and various structure/property relations, describes membrane fabrication technology, describes polymers believed to be used commercially for gas separations, and discusses some challenges associated with membrane materials development. A description of new classes of polymers being considered for gas separations, largely to overcome existing challenges or access applications that are not yet practiced commercially, is also provided. Some classes of polymers discussed in this review that have been the focus of much recent work include thermally rearranged (TR) polymers, polymers of intrinsic microporosity (PIMs), room-temperature ionic liquids (RTILs), perfluoropolymers, and high-performance polyimides.
Antineoplastic agents 460. Synthesis of combretastatin A-2 prodrugs. (2002)

The original synthesis of combretastatin A-2 (1a) was modified to provide an efficient scale-up procedure for obtaining this antineoplastic stilbene. Subsequent conversion to a useful prodrug was accomplished by phosphorylation employing in situ formation of dibenzylchlorophosphite followed by cleavage of the benzyl ester protective groups with bromotrimethylsilane to afford the phosphoric acid intermediate 11. The latter was immediately treated with sodium methoxide to complete a practical route to the disodium phosphate prodrug (2a). The phosphoric acid precursor (11) of phosphate 2a was employed in a parallel series of reactions to produce a selection of metal and ammonium cation prodrug candidates. Each of the phosphate salts (2a-q) was evaluated with respect to relative solubility behavior, cancer cell growth inhibition and antimicrobial activity.
Production of Conducting Polymer Nanowires for Use as Intravascular Neural Recording Electrodes (2006)

A crucial factor limiting the adoption of brain-machine interfaces is the invasiveness of conventionally implanted cortical microelectrode arrays. Recently, Llinas et al. proposed a new, less invasive type of multielectrode neural recording, using sub-micrometer insulated nanowires placed in the capillary bed of the central nervous system [1]. Initial experiments using platinum wires were plagued by wire stiffness and metal fatigue. We report the production of conductive, insulated polymer nanowires with a doped polyaniline core and a poly(ethylene oxide) shell by coaxial electrospinning. These fibers have continuous insulated conducting polymer lengths over 10 mm, and a core polymer conductivity of 3 S/m.
Two new parameters to assess anterior chamber angle for SS-OCT images (2013)

In this paper, we propose an automatic quantitative assessment method of the anterior chamber angle of the eye using swept source optical coherence tomography (SS-OCT) images. The angle structure was assessed by two new parameters: 1) mean of continuous measurement of angle opening distance (mAOD) centered on Schwalbe's line; and 2) area of the anterior chamber (AAC). In this research, two hundred and fifty-two SS-OCT images were analyzed. The measurements of open and closed angle in SS-OCT images were compared with that of Schwalbe's line based single angle assessment (AOD_sl, ARA_sl). Our continuous based AOD measurement shows over 93% correlation with the single based AOD angle assessment but provides a more reliable and accurate measurement for open and closed angle SS-OCT images. These newly proposed parameters give a better representation of the angle width for capturing the nature of the angle dynamics involved in different forms of angle closure or open angle glaucoma.
ChemInform Abstract: Rechargeable Magnesium Battery: Current Status and Key Challenges for the Future (2015)

Abstract Review: 239 refs.
Generation, specificity, and function of CD8<sup>+</sup> T cells in <i>Trypanosoma cruzi</i> infection (2004)

CD8(+) T cells are crucial to the control of Trypanosoma cruzi infection and probably act via multiple mechanisms, the most important being the production of interferon-gamma (IFN-gamma). In the absence of CD8(+) T cells, mice quickly succumb to the infection or develop a more severe chronic disease. Reduced production of IFN-gamma by CD8(+) T cells is also associated with increased severity of chagasic disease in humans. CD8(+) T cells in chronic T. cruzi infection are maintained as effector memory cells, undergo rapid expansion, and demonstrate effector functions following re-exposure to antigen. However, the initial generation of T. cruzi-specific CD8(+) T-cell responses appears to be relatively slow to develop. In addition, the expression of the effector function of the CD8(+) T cells is compromised in some tissues, particularly in muscle. The targets of effective CD8(+) T-cell responses in T. cruzi infection are multiple and varied, and they represent some of the best vaccine candidates described to date. Further analysis of CD8(+) T cells will provide insight into the disease process in T. cruzi infection and should identify methods to assess and enhance immunity to T. cruzi infection and protection from the symptoms of Chagas' disease.
Experimental verification of PSM polarimetry: monitoring polarization at 193nm high-NA with phase shift masks (2006)

The initial experimental verification of a polarization monitoring technique is presented. A series of phase shifting mask patterns produce polarization dependent signals in photoresist and are capable of monitoring the Stokes parameters of any arbitrary illumination scheme. Experiments on two test reticles have been conducted. The first reticle consisted of a series of radial phase gratings (RPG) and employed special apertures to select particular illumination angles. Measurement sensitivities of about 0.3 percent of the clear field per percent change in polarization state were observed. The second test reticle employed the more sensitive proximity effect polarization analyzers (PEPA), a more robust experimental setup, and a backside pinhole layer for illumination angle selection and to enable characterization of the full illuminator. Despite an initial complication with the backside pinhole alignment, the results correlate with theory. Theory suggests that, once the pinhole alignment is corrected in the near future, the second reticle should achieve a measurement sensitivity of about 1 percent of the clear field per percent change in polarization state. This corresponds to a measurement of the Stokes parameters after test mask calibration, to within about 0.02 to 0.03. Various potential improvements to the design, fabrication of the mask, and experimental setup are discussed. Additionally, to decrease measurement time, a design modification and double exposure technique is proposed to enable electrical detection of the measurement signal.
A VOP generation tool: automatic segmentation of moving objects in image sequences based on spatio-temporal information (1999)

The new MPEG-4 video coding standard enables content-based functionalities. In order to support the philosophy of the MPEG-4 visual standard, each frame of video sequences should be represented in terms of video object planes (VOPs). In other words, video objects to be encoded in still pictures or video sequences should be prepared before the encoding process starts. Therefore, it requires a prior decomposition of sequences into VOPs so that each VOP represents a moving object. This paper addresses an image segmentation method for separating moving objects from the background in image sequences. The proposed method utilizes the following spatio-temporal information. (1) For localization of moving objects in the image sequence, two consecutive image frames in the temporal direction are examined and a hypothesis testing is performed by comparing two variance estimates from two consecutive difference images, which results in an F-test. (2) Spatial segmentation is performed to divide each image into semantic regions and to find precise object boundaries of the moving objects. The temporal segmentation yields a change detection mask that indicates moving areas (foreground) and nonmoving areas (background), and spatial segmentation produces spatial segmentation masks. A combination of the spatial and temporal segmentation masks produces VOPs faithfully. This paper presents various experimental results.
ChemInform Abstract: Novel Substituted Adamantane‐spiro‐5‐oxazolidine Derivatives. (1987)

Abstract The aminomethylhydroxyadamantane (I) is cyclized with phosgene (II) or the ketones (VIII) to produce the spirooxazolidinone (IIIa) and the bisspiro compounds (IX) respectively.
Improving Cell‐Free Protein Synthesis through Genome Engineering of <i>Escherichia coli</i> Lacking Release Factor 1 (2015)

Site-specific incorporation of non-standard amino acids (NSAAs) into proteins opens the way to novel biological insights and applications in biotechnology. Here, we describe the development of a high yielding cell-free protein synthesis (CFPS) platform for NSAA incorporation from crude extracts of genomically recoded Escherichia coli lacking release factor 1. We used genome engineering to construct synthetic organisms that, upon cell lysis, lead to improved extract performance. We targeted five potential negative effectors to be disabled: the nuclease genes rna, rnb, csdA, mazF, and endA. Using our most productive extract from strain MCJ.559 (csdA(-) endA(-)), we synthesized 550±40 μg mL(-1) of modified superfolder green fluorescent protein containing p-acetyl-L-phenylalanine. This yield was increased to ∼1300 μg mL(-1) when using a semicontinuous method. Our work has implications for using whole genome editing for CFPS strain development, expanding the chemistry of biological systems, and cell-free synthetic biology.
The Use of Fast and Thermal Neutron Detectors Based on Oxide Scintillators in Inspection Systems for Prevention of Illegal Transportation of Radioactive Substances (2010)

We present results of our studies on the practical application of an efficient method for detection of fast and thermal neutrons using the process of inelastic scattering on nuclei present in inorganic scintillators. The most obvious field of practical application for this method is inspection systems for the prevention of illegal transportation of radioactive substances. Especially promising is the design of a small-sized neutron detector for portable radioactive material detection systems using “scintillator-avalanche photodiode” assemblies.
When self-assembly meets biology: luminescent platinum complexes for imaging applications (2014)

Luminescent platinum complexes have attractive chemical and photophysical properties such as high stability, emission in the visible region, high emission quantum yields and long excited state lifetimes. However the absorption spectrum of the compounds in the UV region, preventing their excitation in the harmless visible/red region, as well as the strong quenching of the luminescent triplet state, caused by dioxygen in water and biological fluids, reduces their possible applications for imaging. Therefore a possible solution to these drawbacks is to take advantage of the high tendency of such square planar compounds to self-assemble in supramolecular structures. The assemblies can be considered new chemical species with enhanced and tunable properties. Furthermore the assembly and disassembly process can be explored as a tool to obtain dynamic labels that can be applied in biomedicine. The change in color, the turn on and off of luminescence but also of the reactivity, the protection from quenching and environmental degradation are some of the attractive properties connected to the aggregation of the complexes.
The Temporal Logic of Causal Structures (2012)

Computational analysis of time-course data with an underlying causal structure is needed in a variety of domains, including neural spike trains, stock price movements, and gene expression levels. However, it can be challenging to determine from just the numerical time course data alone what is coordinating the visible processes, to separate the underlying prima facie causes into genuine and spurious causes and to do so with a feasible computational complexity. For this purpose, we have been developing a novel algorithm based on a framework that combines notions of causality in philosophy with algorithmic approaches built on model checking and statistical techniques for multiple hypotheses testing. The causal relationships are described in terms of temporal logic formulae, reframing the inference problem in terms of model checking. The logic used, PCTL, allows description of both the time between cause and effect and the probability of this relationship being observed. We show that equipped with these causal formulae with their associated probabilities we may compute the average impact a cause makes to its effect and then discover statistically significant causes through the concepts of multiple hypothesis testing (treating each causal relationship as a hypothesis), and false discovery control. By exploring a well-chosen family of potentially all significant hypotheses with reasonably minimal description length, it is possible to tame the algorithm's computational complexity while exploring the nearly complete search-space of all prima facie causes. We have tested these ideas in a number of domains and illustrate them here with two examples.
Diphone synthesis using unit selection. (1998)

This paper describes an experimental AT&amp;T concatenative synthesis system using unit selection, for which the basic synthesis units are diphones. The synthesizer may use any of the data from a large database of utterances. Since there are in general multiple instances of each concatenative unit, the system performs dynamic unit selection. Selection among candidates is done dynamically at synthesis, in a manner that is based on and extends unit selection implemented in the CHATR synthesis system [1][4]. Selected units may be either phones or diphones, and they can be synthesized by a variety of methods, including PSOLA [5], HNM [11], and simple unit concatenation. The AT&amp;T system, with CHATR unit selection, was implemented within the framework of the Festival Speech Synthesis System [2]. The voice database amounted to approximately one and one-half hours of speech and was constructed from read text taken from three sources. The first source was a portion of the 1989 Wall Street Journal m...