gebhart/patrepeval huggingface dataset:
Impact of Somatosensory Evoked Potential Monitoring on Cervical Surgery (2003)

Controversy still exists about the necessity of somatosensory evoked potential (SSEP) monitoring during cervical surgery. The purpose of this prospective study is to determine the impact of SSEP monitoring on anterior cervical surgery. Intraoperative SSEP monitoring was performed in 100 patients treated by an anterior cervical approach. The patients were divided into three groups according to their preoperative clinical condition. Somatosensory evoked potential monitoring was performed during five stages of the procedure: M1, after the induction of anesthesia; M2, during positioning; M3, during distraction of the intervertebral space; M4, throughout decompression; and M5, during graft placement. Normal SSEPs were obtained during M1 from all the patients in group 2. Pathologic SSEPs were recorded at M1 in 45 patients from group 1. No SSEPs were recorded at M1 in six patients in group 3. A deterioration of the SSEPs was observed in 35 patients during M2. Deteriorated SSEPs were observed during M3 in 14 patients. No deterioration of the SSEPs was recorded during M4. Intraoperative SSEP monitoring is easy to perform and helps to increase safety during anterior cervical surgery. Critical phases of the surgical procedure were identified and the surgical strategy was modified as a result of this study.
Pathogen‐Mimicking Polymeric Nanoparticles based on Dopamine Polymerization as Vaccines Adjuvants Induce Robust Humoral and Cellular Immune Responses (2016)

Aiming to enhance the immunogenicity of subunit vaccines, a novel antigen delivery and adjuvant system based on dopamine polymerization on the surface of poly(D,L-lactic-glycolic-acid) nanoparticles (NPs) with multiple mechanisms of immunity enhancement is developed. The mussel-inspired biomimetic polydopamine (pD) not only serves as a coating to NPs but also functionalizes NP surfaces. The method is facile and mild including simple incubation of the preformed NPs in the weak alkaline dopamine solution, and incorporation of hepatitis B surface antigen and TLR9 agonist unmethylated cytosine-guanine (CpG) motif with the pD surface. The as-constructed NPs possess pathogen-mimicking manners owing to their size, shape, and surface molecular immune-activating properties given by CpG. The biocompatibility and biosafety of these pathogen-mimicking NPs are confirmed using bone marrow-derived dendritic cells. Pathogen-mimicking NPs hold great potential as vaccine delivery and adjuvant system due to their ability to: 1) enhance cytokine secretion and immune cell recruitment at the injection site; 2) significantly activate and maturate dendritic cells; 3) induce stronger humoral and cellular immune responses in vivo. Furthermore, this simple and versatile dopamine polymerization method can be applicable to endow NPs with characteristics to mimic pathogen structure and function, and manipulate NPs for the generation of efficacious vaccine adjuvants.
Investigations of the growth conditions for GaN‐bulk crystals grown by the sublimation technique (2007)

Abstract Spontaneous nucleation of GaN and the growth of thick polycrystalline layers by the sublimation technique in nitrogen atmosphere have been studied as a function of growth environment and conditions. Average growth rates higher than 10 microns per hour were realised by reaction of gallium, evaporated from an elemental source, with nitrogen from ammonia gas. The growth temperature was in the range of 1000 – 1300 °C; the pressure was varied between 200 ‐ 800 hPa and the ammonia flow between 50‐500 sccm min –1 . It was found that the growth rate only moderately decreases during a long‐term experiment when using an optimised set of growth parameters. Furthermore, a strong influence of the growth environment on the growth rate and impurity content was found. (© 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Optimal Coverage Path Planning for Arable Farming on 2D Surfaces (2010)

With the rapid adoption of automatic guidance systems in agriculture, automated path planning has great potential to further optimize field operations. Field operations should be done in a manner that minimizes time and travel over field surfaces and should be coordinated with specific field operation requirements, machine characteristics, and topographical features of arable lands. To reach this goal, an intelligent coverage path planning algorithm is the key. To determine the full coverage pattern of a given field by using boustrophedon paths, it is necessary to know whether to and how to decompose a field into sub-regions and how to determine the travel direction within each sub-region. A geometric model was developed to represent this coverage path planning problem, and a path planning algorithm was developed based on this geometric model. The search mechanism of the algorithm was guided by a customized cost function resulting from the analysis of different headland turning types and implemented with a divide-and-conquer strategy. The complexity of the algorithm was analyzed, and methods for reducing the computational time are discussed. Field examples with complexity ranging from a simple convex shape to an irregular polygonal shape that has multiple obstacles within its interior were tested with this algorithm. The results were compared with other reported approaches or farmers' recorded patterns. These results indicate that the proposed algorithm was effective in producing optimal field decomposition and coverage path direction in each sub-region.
Interaction of Subtilisin BPN' and Recombinant Streptomyces Subtilisin Inhibitors with Substituted P1 Site Residues1 (1993)

Kinetic analysis was performed on the interaction between subtilisin BPN' and recombinant species of a proteinaceous proteinase inhibitor, Streptomyces subtilisin inhibitor (SSI), of which the P1 site amino acid residue, Met73, was replaced by site-directed mutagenesis. The inhibitor constant, Ki, was determined from the residual enzyme activity by using a peptide substrate. The rate constant of binding, kon, and the rate constant of dissociation, koff, were determined from a progress curve of the substrate hydrolysis in the presence of the inhibitor by using newly derived equations. A recombinant SSI in which Met73 was replaced by Ile showed an affinity (1/Ki) toward subtilisin BPN' of only about 7% of that of the wild-type SSI, and the kinetic analysis revealed that the increase of koff was responsible for this difference. The affinity of other SSI mutants in which Met73 was replaced by Glu or Asp decreased significantly as pH became increasingly alkaline. The decrease in the affinity of these recombinants was due to the decrease of kon rather than the increase of koff. Stopped-flow studies revealed that the binding reaction was reconcilable with a two-step mechanism, and the kinetic parameters for each step were obtained for the binding of the enzyme and recombinant SSIs.
Advances in Antisense Oligonucleotide Development for Target Identification, Validation, and as Novel Therapeutics (2008)

Antisense oligonucleotides (As-ODNs) are single stranded, synthetically prepared strands of deoxynucleotide sequences, usually 18-21 nucleotides in length, complementary to the mRNA sequence of the target gene. As-ODNs are able to selectively bind cognate mRNA sequences by sequence-specific hybridization. This results in cleavage or disablement of the mRNA and, thus, inhibits the expression of the target gene. The specificity of the As approach is based on the probability that, in the human genome, any sequence longer than a minimal number of nucleotides (nt), 13 for RNA and 17 for DNA, normally occurs only once. The potential applications of As-ODNs are numerous because mRNA is ubiquitous and is more accessible to manipulation than DNA. With the publication of the human genome sequence, it has become theoretically possible to inhibit mRNA of almost any gene by As-ODNs, in order to get a better understanding of gene function, investigate its role in disease pathology and to study novel therapeutic targets for the diseases caused by dysregulated gene expression. The conceptual simplicity, the availability of gene sequence information from the human genome, the inexpensive availability of synthetic oligonucleotides and the possibility of rational drug design makes As-ODNs powerful tools for target identification, validation and therapeutic intervention. In this review we discuss the latest developments in antisense oligonucleotide design, delivery, pharmacokinetics and potential side effects, as well as its uses in target identification and validation, and finally focus on the current developments of antisense oligonucleotides in therapeutic intervention in various diseases.
Platelet-derived LIGHT induces inflammatory responses in endothelial cells and monocytes (2006)

Traditionally, platelets are known to play an important role in hemostasis, thrombosis, and wound healing, but increasing evidence suggests that activated platelets also may promote inflammation. Platelet-induced modulation of inflammation seems to involve platelet expression of ligands in the tumor necrosis factor (TNF) superfamily such as CD40 ligand and Fas ligand. The present study demonstrates that LIGHT, another member of the TNF superfamily, is associated with platelets and is released as a soluble ligand on platelet activation. The release of LIGHT involves GP IIb/IIIa-dependent mechanisms and action of metal-dependent proteases as well as intracellular processes such as actin polymerization. We also report that platelet-derived LIGHT is biologically active and can induce an inflammatory response in monocytes and particularly within endothelial cells measured as up-regulation of adhesion molecules and release of chemokines. Moreover, we demonstrate that thrombus material, obtained at the site of plaque rupture in patients with acute myocardial infarction, contains platelet-associated LIGHT, suggesting that LIGHT-mediated inflammation also is operating in vivo within an inflamed and thrombotic vessel wall. The data may suggest a pathogenic role for platelet-derived LIGHT in atherogenesis and plaque destabilization as well as in other inflammatory disorders involving leukocyte infiltration into the vessel wall.
High data rate Reed-Solomon encoding and decoding using VLSI technology (1987)

International Telemetering Conference Proceedings / October 26-29, 1987 / Town and Country Hotel, San Diego, California
High mobility group box 1 orchestrates tissue regeneration via CXCR4 (2017)

Inflammation and tissue regeneration follow tissue damage, but little is known about how these processes are coordinated. High Mobility Group Box 1 (HMGB1) is a nuclear protein that, when released on injury, triggers inflammation. We previously showed that HMGB1 with reduced cysteines is a chemoattractant, whereas a disulfide bond makes it a proinflammatory cytokine. Here we report that fully reduced HMGB1 orchestrates muscle and liver regeneration via CXCR4, whereas disulfide HMGB1 and its receptors TLR4/MD-2 and RAGE (receptor for advanced glycation end products) are not involved. Injection of HMGB1 accelerates tissue repair by acting on resident muscle stem cells, hepatocytes, and infiltrating cells. The nonoxidizable HMGB1 mutant 3S, in which serines replace cysteines, promotes muscle and liver regeneration more efficiently than the wild-type protein and without exacerbating inflammation by selectively interacting with CXCR4. Overall, our results show that the reduced form of HMGB1 coordinates tissue regeneration and suggest that 3S may be used to safely accelerate healing after injury in diverse clinical contexts.
SD3: A Scalable Approach to Dynamic Data-Dependence Profiling (2010)

As multicore processors are deployed in mainstream computing, the need for software tools to help parallelize programs is increasing dramatically. Data-dependence profiling is an important technique to exploit parallelism in programs. More specifically, manual or automatic parallelization can use the outcomes of data-dependence profiling to guide where to parallelize in a program. However, state-of-the-art data-dependence profiling techniques are not scalable as they suffer from two major issues when profiling large and long-running applications: (1) runtime overhead and (2) memory overhead. Existing data-dependence profilers are either unable to profile large-scale applications or only report very limited information. In this paper, we propose a scalable approach to data-dependence profiling that addresses both runtime and memory overhead in a single framework. Our technique, called SD <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sup> , reduces the runtime overhead by parallelizing the dependence profiling step itself. To reduce the memory overhead, we compress memory accesses that exhibit stride patterns and compute data dependences directly in a compressed format. We demonstrate that SD <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sup> reduces the runtime overhead when profiling SPEC 2006 by a factor of 4.1× and 9.7× on eight cores and 32 cores, respectively. For the memory overhead, we successfully profile SPEC 2006 with the reference input, while the previous approaches fail even with the train input. In some cases, we observe more than a 20× improvement in memory consumption and a 16× speedup in profiling time when 32 cores are used.
Parameters affecting the enzyme-linked immunosorbent assay of immunoglobulin G antibody to a rough mutant of Salmonella minnesota (1978)

An enzyme-linked immunosorbent assay was developed to detect rabbit immunoglobulin G antibodies against purified lipopolysaccharide of the rough Re mutant of Salmonella minnesota. The time necessary for each step was investigated, and this resulted in a test that could be completed in 1 working day. Serial dilutions of rabbit sera drawn throughout immunization with the Re mutant revealed an elimination of the prozone effect upon prolonged immunization. We interpret this to demonstrate an increase in immunoglobulin G affinity for the lipopolysaccharide on prolonged immunization. We propose that the enzyme-linked immunosorbent assay prozone effect be investigated for use as a measure of antibody affinity.
Difficulties with tooth protectors in endotracheal intubation (1988)

The suitability of three tooth protectors for routine use during endotracheal intubation was studied in 300 consecutive patients undergoing elective operations under general anaesthesia. The main disadvantages of the protectors were lack of space and the consequent difficulty of guiding the endotracheal tube into the larynx, and poor visibility, especially when the Camo protector was used. These difficulties could be avoided in most cases by cutting off the right angle of the Camo protector. The less experienced anaesthesiologists especially had difficulties with the protectors: 20% of patients in the Camo group were considered impossible to intubate unless the protector was removed. The silicone inlay of the Camo protector melts and becomes adhesive at body temperature, which makes its prolonged use hazardous. Two patients lost a maxillary incisor despite the proper use of a protector (Denex). Thus the use of a tooth protector alone does not guarantee avoidance of dental trauma. Better results could be obtained by improving the design of the protectors and by careful pre-anaesthetic dental examination.
Minimal definition of the imprinting center and fixation of chromosome 15q11-q13 epigenotype by imprinting mutations. (1996)

Patients with disorders involving imprinted genes such as Angelman syndrome (AS) and Prader-Willi syndrome (PWS) can have a mutation in the imprinting mechanism. Previously, we identified an imprinting center (IC) within chromosome 15q11-ql3 and proposed that IC mutations block resetting of the imprint, fixing on that chromosome the parental imprint (epigenotype) on which the mutation arose. We now describe four new microdeletions of the IC, the smallest (6 kb) of which currently defines the minimal region sufficient to confer an AS imprinting mutation. The AS deletions all overlap this minimal region, centromeric to the PWS microdeletions, which include the first exon of the SNRPN gene. None of five genes or transcripts in the 1.0 Mb vicinity of the IC (ZNF127, SNRPN, PAR-5, IPW, and PAR-1), each normally expressed only from the paternal allele, was expressed in cells from PWS imprinting mutation patients. In contrast, AS imprinting mutation patients show biparental expression of SNRPN and IPW but must lack expression of the putative AS gene 250-1000 kb distal of the IC. These data strongly support a model in which the paternal chromosome of these PWS patients carries an ancestral maternal epigenotype, and the maternal chromosome of these AS patients carries an ancestral paternal epigenotype. The IC therefore functions to reset the maternal and paternal imprints throughout a 2-Mb imprinted domain within human chromosome 15q11-q13 during gametogenesis.
Metallothionein gene expression in bladder cancer exposed to cisplatin. (1993)

Some 68% of bladder tumors will respond to cisplatin-based chemotherapy but only 30% will have a durable response. Recent studies have suggested that the metallothionein (MT) gene may produce cisplatin drug resistance in cell lines. To determine the role of MT gene overexpression in human tumors resistant to cisplatin, we evaluated 19 bladder tumors, seven of which had been exposed to cisplatin, for MT mRNA expression. By Northern analysis, four of the seven tumors exposed to cisplatin overexpressed the MT gene compared to untreated tumors. Of the three treated tumors without MT overexpression, one was a relapse 4 yr after the last dose of cisplatin and the other two received only one dose of chemotherapy. MT gene overexpression was found in some tumors that had failed cisplatin chemotherapy and may be a mechanism for drug resistance in bladder cancer.
TCP Westwood: End-to-End Bandwidth Estimation for Enhanced Transport over Wireless Links (2002)

TCP Westwood (TCPW) is a sender-side modification of the TCP congestion window algorithm that improves upon the performance of TCP Reno in wired as well as wireless networks. The improvement is most significant in wireless networks with lossy links. In fact, TCPW performance is not very sensitive to random errors, while TCP Reno is equally sensitivity to random loss and congestion loss and cannot discriminate between them. Hence, the tendency of TCP Reno to overreact to errors. An important distinguishing feature of TCP Westwood with respect to previous wireless TCP &quot;extensions&quot; is that it does not require inspection and/or interception of TCP packets at intermediate (proxy) nodes. Rather, TCPW fully complies with the end-to-end TCP design principle. The key innovative idea is to continuously measure at the TCP sender side the bandwidth used by the connection via monitoring the rate of returning ACKs. The estimate is then used to compute congestion window and slow start threshold after a congestion episode, that is, after three duplicate acknowledgments or after a timeout. The rationale of this strategy is simple: in contrast with TCP Reno which &quot;blindly&quot; halves the congestion window after three duplicate ACKs, TCP Westwood attempts to select a slow start threshold and a congestion window which are consistent with the effective bandwidth used at the time congestion is experienced. We call this mechanism faster recovery. The proposed mechanism is particularly effective over wireless links where sporadic losses due to radio channel problems are often misinterpreted as a symptom of congestion by current TCP schemes and thus lead to an unnecessary window reduction. Experimental studies reveal improvements in throughput performance, as well as in fairness. In addition, fri...
Enhancing energy efficiency of processor-based embedded systems through post-fabrication ISA extension (2008)

Application-specific instruction set extension is an effective technique for reducing accesses to components such as on-and off-chip memories, register file and enhancing the energy efficiency.However, the addition of custom functional units to the base processor is required for supporting custom instructions, which due to the increase of manufacturing and design costs in new nanometer-scale technologies and shorter time-to-market, is becoming an issue.To address above issues, in our proposed approach, an optimized reconfigurable functional unit is used instead, and instruction set customization is done after chip-fabrication.Therefore, while maintaining the flexibility of a conventional microprocessor, the low-energy feature of customization is applicable.Experimental results show that the maximum and average energy savings are 67% and 22%, respectively for our proposed architecture framework.
Development of a Test Procedure for Quantifying Performance Benefits of Solar Control Glazings on Occupant Comfort (1991)

<div class="htmlview paragraph">The evaluation of the performance benefits of solar load reducing glazings using production vehicles is key to the establishment of the product cost/benefit ratio. Climatic windtunnels normally used to evaluate heat gain and vehicle cooldown can not provide true solar simulation. Comparative testing using a test car and a control vehicle must therefore be conducted outside in uncontrollable ambient conditions.</div> <div class="htmlview paragraph">The subject paper deals with the development of a testing methodology capable of quantifying thermal performance differences, as low as 5%, resulting from component differences, including glazings. The procedure described includes the use of B &amp; K Thermal Comfort Meters to standardize the refrigeration system performance and to evaluate the rate of vehicle interior cooldown. Data taken during summer test programs in the Southwest for evaluation of heat absorbing glazings will be reviewed. Data to be presented include interior air and surface temperatures during soak testing and cooldown comparisons under city drive conditions. The general relationship of comfort meter data to driver comfort index will also be discussed.</div>
Ion selectivity of graphene nanopores (2016)

As population growth continues to outpace development of water infrastructure in many countries, desalination (the removal of salts from seawater) at high energy efficiency will likely become a vital source of fresh water. Due to its atomic thinness combined with its mechanical strength, porous graphene may be particularly well-suited for electrodialysis desalination, in which ions are removed under an electric field via ion-selective pores. Here, we show that single graphene nanopores preferentially permit the passage of K(+) cations over Cl(-) anions with selectivity ratios of over 100 and conduct monovalent cations up to 5 times more rapidly than divalent cations. Surprisingly, the observed K(+)/Cl(-) selectivity persists in pores even as large as about 20 nm in diameter, suggesting that high throughput, highly selective graphene electrodialysis membranes can be fabricated without the need for subnanometer control over pore size.