gebhart/patrepeval huggingface dataset:
Protection of CD8+ T cells from activation-induced cell death by IL-18 (2007)

Role of IL-18 on proliferation and survival of CD8+ T cells, activated by immobilized anti-CD3 antibody (anti-CD3), was examined. Proliferation and survival of activated T cells, especially that of CD8+ T cells, were impaired by IL-18 deficiency [IL-18 knockout (KO)]. After 3 days of culture with anti-CD3, the number of living CD8+ T cells from IL-18KO mice was approximately 25% of that from wild-type (WT) mice but was increased to the same level as WT cells by the addition of IL-18. The expression of IL-18 receptors (IL-18Rs), particularly IL-18Rbeta chain, in naïve CD8+ T cells was very low but elevated after stimulation with anti-CD3. Blockade of IL-18R by anti-IL-18R antibody on activated WT CD8+ T cells resulted in reduction of living cells, suggesting that IL-18 promotes survival of proliferating CD8+ T cells. Levels of Bcl-2 in activated IL-18KO CD8+ T cells were lower than those in WT cells but were raised by exogenous IL-18. Blockade of IL-18R on WT CD8+ T cells decreased the expression of surface markers CD122 and CD94, which are related to cell viability, and the expression of these markers was increased by exogenous IL-18 in IL-18KO cells. These results suggest that IL-18 acts directly on activated CD8+ T cells through IL-18Rs and promotes their survival to expand the population.
Effects of Intra-articular Botulinum Toxin Type A in an Equine Model of Acute Synovitis (2007)

Our findings support the idea that BoNT-A can attenuate lameness in an equine model of acute synovitis. Our findings further suggest that BoNT-A might be a potential new treatment for painful arthritis; this warrants further study.
The effect of injection of powdered biomaterials on mouse peritoneal cell populations (1987)

Abstract The authors suggest an experimental model for the assaying, before implantation, of the tissue reaction that wear particles from artificial joints can cause in the human body. Mice were intraperitoneally injected with biomaterial powders in suspension in saline. The materials used were chromium, chromium oxide, nickel, nickel oxide, aluminium, alumina, alumina‐titania, cobalt, molybdenum, titanium, silver, zirconium oxide, iron oxide, and stainless steel. Peritoneal lavage was performed a week after injection. A thorough morphological and quantitative analysis of the cell suspension thus obtained was made both immediately after collection and after a 24 h culture.
Can laboratory investigations help us to decide when to discontinue larval therapy? (2004)

Although the methodological limitations of this open-label non-comparative cohort study preclude a definite conclusion, we believe that laboratory investigations, particularly leucocyte count, can guide the decision of when to discontinue larval therapy. However, this objective parameter cannot replace clinical judgement.
Three-dimensional microwave tomography: experimental imaging of phantoms and biological objects (2000)

Microwave tomographic experiments have been performed on a three-dimensional (3-D) phantom and excised canine heart using a 3-D system operating at frequency of 2.4 GHz. A modified gradient reconstruction approach has been employed for the 3-D image reconstruction. To compare two-dimensional (2-D) and 3-D approaches, we also performed 2-D image reconstruction using an approach based on the Newton method. Experimental data acquired on experimental phantoms were analyzed using both 2-D and 3-D reconstruction approaches. High-quality images were reconstructed using the 3-D approach. The reconstruction procedure failed when the 2-D approach was applied to reconstruct images of the 3-D object. An image of the dielectric properties of the excised canine heart was obtained using a 3-D reconstruction approach. Images successfully revealed a complex internal structure of the heart, including both right-hand side and left-hand side ventricles.
Interleukin‐17–producing T cells are enriched in the joints of children with arthritis, but have a reciprocal relationship to regulatory T cell numbers (2008)

This study is the first to define the frequency and characteristics of "Th17" cells in JIA. We suggest that these highly proinflammatory cells contribute to joint pathology, as indicated by relationships with clinical phenotypes, and that the balance between IL-17+ T cells and Treg cells may be critical to outcome.
Chemiresistive Sensing with Chemically Modified Metal and Alloy Nanoparticles (2011)

This review describes the use of chemically modified pure and alloyed metal nanoparticles for chemiresistive sensing applications. Chemically modified metal nanoparticles consist of a pure or alloyed metallic core with some type of chemical coating. Researchers have studied the electronic properties of 1D, 2D, and 3D assemblies of chemically modified metal nanoparticles, and even single individual nanoparticles. The interaction with the analyte alters the conductivity of the sensitive material, providing a signal to measure the analyte concentration. This review focuses on chemiresistive sensing of a wide variety of gas- and liquid-phase analytes with metal nanoparticles coated with organothiols, ions, polymers, surfactants, and biomolecules. Different strategies used to incorporate chemically modified nanoparticles into chemiresistive sensing devices are reviewed, focusing on the different types of metal and alloy compositions, coatings, methods of assembly, and analytes (vapors, gases, liquids, biological materials), along with other important factors.
Oral Administration of Nicotinamide Mononucleotide Is Safe and Efficiently Increases Blood Nicotinamide Adenine Dinucleotide Levels in Healthy Subjects (2022)

Nicotinamide mononucleotide (NNM) is an orally bioavailable NAD<sup>+</sup> precursor that has demonstrated beneficial effects against aging and aging-associated diseases in animal models. NMN is ultimately converted to NAD<sup>+</sup>, a redox cofactor that mediates many metabolic enzymes. NAD<sup>+</sup> also serves as the substrate for poly(ADP-ribose) polymerase (PARP) and sirtuins, and regulates various biological processes, such as metabolism, DNA repair, gene expression, and stress responses. Previous mouse models showed that NMN administration can increase NAD<sup>+</sup> in various organs and ameliorate aging-related diseases, such as obesity, diabetes, heart failure, stroke, kidney failure, and Alzheimer's disease through NAD<sup>+</sup>-mediated pathways. However, evidence of its effect on humans is still scarce. In this study, we conducted a placebo-controlled, randomized, double blind, parallel-group trial to investigate the safety of orally administered NMN and its efficacy to increase NAD<sup>+</sup> levels in thirty healthy subjects. Healthy volunteers received 250 mg/day of NMN (<i>n</i> = 15) or placebo (<i>n</i> = 15) for 12 weeks, and physiological and laboratory tests were performed during this period. In addition, NAD<sup>+</sup> and its related metabolites in whole blood were examined. Oral supplementation of NMN for 12 weeks caused no abnormalities in physiological and laboratory tests, and no obvious adverse effects were observed. NAD<sup>+</sup> levels in whole blood were significantly increased after NMN administration. We also observed the significant rise in nicotinic acid mononucleotide (NAMN) levels, but not in NMN. We also found that the increased amount of NAD<sup>+</sup> was strongly correlated with pulse rate before the administration of NMN. These results suggest that oral administration of NMN is a safe and practical strategy to boost NAD<sup>+</sup> levels in humans. <b>Clinical Trial Registration:</b> JRCT [https://jrct.niph.go.jp/], identifier: [jRCTs041200034].
Holding Power and Reinforcement of Cancellous Screws in Human Bone (1992)

The authors report an in vitro biomechanical evaluation of a biodegradable material that might be used for the reinforcement of surgical screws in fractures involving severely osteoporotic bone. The material is a particulate composite with a matrix phase consisting of a hydrolyzable prepolymer, polypropylene fumarate (PPF), crosslinked with methacrylate monomer, and a particulate phase consisting of tricalcium phosphate and calcium carbonate. Pullout force and stripping load of cancellous screws were determined along with screw pullout force before and after reinforcement with either polymethylmethacrylate (PMMA) or PPF composite. Pullout force was moderately correlated (R2 = 0.59) with apparent density by a power law relationship of the form 0.065p1.37-1.77. Stripping load was strongly correlated (R2 = 0.91) with apparent density by a power law of the form 0.13p1.35-93.8. Mean pullout force before and after reinforcement with PMMA was 382 +/- 100 N (mean +/- standard deviation) and 879 +/- 315 N, respectively. Mean pullout force before and after reinforcement with PPF composite was 571 +/- 294 N and 829 +/- 354 N, respectively. Although the increase in pullout force with cement reinforcement was highly significant in both cases, the magnitude of the increase did not depend on the type of cement. Thus PPF seems to provide reinforcement that is equivalent to that provided by PMMA.
High-performance optical local and metropolitan area networks: enhancements of FDDI and IEEE 802.6 DQDB (2003)

The Fiber Distributed Data Interface (FDDI) and the IEEE 802.6 Distributed Queueing Dual Bus (DQDB) are emerging standards for high-speed (45-150 Mb/s) local and metropolitan area networks. Several ways of building on these emerging standards to significantly increase the achievable throughput and lower the end-to-end delay are described. Without increasing the number of transceivers or their rate, substantial throughput increases are obtained by a highly concurrent logical interconnection pattern of user nodes, and the end-to-end delay is decreased by the use of more efficient media-access techniques. The most promising architecture is a multiconnected ring having only two transmitters and two receivers per node, where each node needs to handle or process only a small fraction of the network traffic. In one example, a 24-node, distributed, packet-switched network, with only two 100-Mb/s transmitters and two 100-Mb/s receivers per node, that has a throughput of 1.5 GB/s (15 time the throughput of FDDI) is described. Such a system has the potential to be a follow-on standard to FDDI (or IEEE 802.6) or to provide a high-performance local/metropolitan area network that can interwork with standard systems.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Conservation practice could benefit from routine testing and publication of management outcomes (2013)

Effective conservation requires a step change in the way practitioners can contribute to science and can have access to research outputs. The journal Conservation Evidence was established in 2004 to help practitioners surmount several obstacles they face when attempting to document the effects of their conservation actions scientifically. It is easily and freely accessible online. It is free to publish in and it enables global communication of the effects of practical trials and experiments, which are virtually impossible to get published in most scientific journals. The driving force behind Conservation Evidence is the need to generate and share scientific information about the effects of interventions.
Real-Time PCR Quantification of Nitrifying Bacteria in a Municipal Wastewater Treatment Plant (2002)

Real-time PCR assays using TaqMan or Molecular Beacon probes were developed and optimized for the quantification of total bacteria, the nitrite-oxidizing bacteria Nitrospira, and Nitrosomonas oligotropha-like ammonia oxidizing bacteria (AOB) in mixed liquor suspended solids (MLSS) from a municipal wastewater treatment plant (WWTP) using a single-sludge nitrification process. The targets for the real-time PCR assays were the 16S rRNA genes (16S rDNA) for bacteria and Nitrospira spp. and the amoA gene for N. oligotropha. A previously reported assay for AOB 16S rDNA was also tested for its application to activated sludge. The Nitrospira 16S rDNA, AOB 16S rDNA, and N. oligotropha-like amoA assays were log-linear over 6 orders of magnitude and the bacterial 16S rDNA real-time PCR assay was log-linear over 4 orders of magnitude with DNA standards. When these real-time PCR assays were applied to DNA extracted from MLSS, dilution of the DNA extracts was necessary to prevent PCR inhibition. The optimal DNA dilution range was broad for the bacterial 16S rDNA (1000-fold) and Nitrospira 16S rDNA assays (2500-fold) but narrow for the AOB 16S rDNA assay (10-fold) and N. oligotropha-like amoA real-time PCR assay (5-fold). In twelve MLSS samples collected over one year, mean cell per L values were 4.3 +/- 2.0 x 10(11) for bacteria, 3.7 +/- 3.2 x 10(10) for Nitrospira, 1.2 +/- 0.9 x 10(10) for all AOB, and 7.5 +/- 6.0 x 10(9) for N. oligotropha-like AOB. The percent of the nitrifying population was 1.7% N. oligotropha-like AOB based on the N. oligotropha amoA assay, 2.9% total AOB based on the AOB 16S rDNA assay, and 8.6% nitrite-oxidizing bacteria based on the Nitrospira 16S rDNA assay. Ammonia-oxidizing bacteria in the wastewater treatment plant were estimated to oxidize 7.7 +/- 6.8 fmol/hr/cell based on the AOB 16S rDNA assay and 12.4 +/- 7.3 fmol/hr/cell based on the N. oligotropha amoA assay.
Extraction of ingredient names from recipes by combining linguistic annotations and CRF selection (2013)

Nutrition and diet have direct and considerable impact on our well-being and health. This field attracts researchers from different areas, such as medicine, nutrition and epidemiology, computer sciences, artificial intelligence and natural language processing (NLP).We process the recipes with NLP methods in order to automatically identify ingredient names within recipes. We propose a hybrid system based on linguistic enrichment of the recipes and selection of the relevant ingredient names with a CRF method. Semantic resources have been specifically built for processing two kinds of information: exact (e.g. quantity expressed in grams or liters, durations expressed in minutes or days) and fuzzy (e.g. quantities expressed in chouilla (smidgeon) and louche (ladle), durations sequenced with aprèes, ensuite, alors que (the, after that, while)). The experiments are performed with French-language textual data. The results demonstrate that the proposed method is useful for searching and managing the recipes.
Multiplexed hydraulic valve actuation using ionic liquid filled soft channels and Braille displays (2007)

Pneumatic actuation with multilayer soft lithography enables operation of up to thousands of valves in parallel using far fewer control lines. However, it is dependent on macroscopic switches and external pressure sources that require interconnects and limit portability. The authors present a more portable and multiplexed valve actuation strategy that uses a grid of mechanically actuated Braille pins to hydraulically, rather than pneumatically, deform elastic actuation channels that act as valves. Experimental and theoretical analyses show that the key to reliable operation of the hydraulic system is the use of nonvolatile ionic liquids as the hydraulic fluid.
Synthesis of non-intrusive concurrent error detection using an even error detecting function (2006)

A new method for synthesizing nonintrusive concurrent error detection (CED) circuitry is presented. The idea is to use single-bit parity to detect all errors affecting an odd number of bits and then synthesize a circuit to detect the even errors. A novel statistical sampling and expanding methodology is proposed for constructing the even error detection circuitry. A major feature of the proposed methodology is that it allows very efficient tradeoffs between error coverage and overhead. While CED schemes that use a fixed checker based on a particular error detecting code are not amenable to simplification without a major impact on coverage, the proposed scheme can easily facilitate significant reductions in overhead with only a small loss in coverage. Experimental results show that the proposed scheme can provide very high levels of soft error protection at a fraction of the cost of duplication
Mid and long infrared detection modules for picosecond range measurements (2009)

Sensitive and broadband detection of MWIR and LWIR radiation with any wavelength within the 2 to 16 μm spectral range and bandwidth from DC to GHz range is reported. Recent efforts have been concentrated on the extension of useful spectrum range above 13 micrometers. This was achieved with improved architecture of the active element, use of monolithic optical immersion technology, enhanced absorption of radiation, dedicated electronics, series connection of small cells and applying more efficient Peltier coolers.
Identification of coenzyme A-related tolmetin metabolites in rats: relationship with reactive drug metabolites (2003)

1. It has recently been proposed that acyl coenzyme A thioesters (acyl-CoAs) of xenobiotic carboxylic acids are electrophilic, reactive metabolites that may react with proteins. 2. The primary objective was to investigate the reactivity of the tolmetin acyl coenzyme A thioester (Tol-CoA). The second objective was to identify and quantify tolmetin (Tol) metabolites in vivo that were formed via Tol-CoA, e.g. the glycine (Tol-Gly) and taurine (Tol-Tau) conjugates. This finding would be indicative of Tol-CoA formation and thus of other acyl-CoA-related reactions that might occur, e.g. covalent binding to proteins. 3. In order to study the chemical reactivity, Tol-CoA (0.5 mM) was incubated with glutathione (5 mM) in a 0.1 M phosphate buffer (pH 7.4) at 37 degrees C. Tol-CoA reacted rapidly with glutathione in vitro to form the S-acyl glutathione conjugate at a rate of 14.9 +/- 0.7 micro M min(-1) (mean +/- SD, n = 3) from 0 to 10 min. Compared with acyl-CoAs of other xenobiotic carboxylic acids, naproxen and clofibric acid, the rate by which Tol-CoA reacted with glutathione was high. 4. Following administration of (3)H-Tol (100 mg kg(-1), 200 micro Ci kg(-1), p.o.) to male Sprague-Dawley rats, Tol-Tau and Tol-Gly were identified in urine by electrospray ionization MS-MS in both positive- and negative-ion modes. The conjugates were only formed at trace levels (< 0.5%). However, the presence of Tol-Tau and Tol-Gly showed the reactive Tol-CoA was formed in vivo.
Person-robot interactions from the robopsychologists' point of view: the robotic psychology and robotherapy approach (2004)

Recent research shows that people perceive and treat robots not just as machines, but also as their companions or artificial partners. Person-robot communication, viewed as a complex interactive system (CIS), is based upon three basic principles: interactivity, equifinality, and multimodality. Classification of artificial creatures from the robopsychologist's point of view divides them into two major groups: assisting robots, which are oriented toward industrial, military, research, medical, and service activities, and interactive stimulation robots, which are designed for social, educational, rehabilitation, therapeutic, and entertainment purposes. The latter class is considered the primary subject for the robotic psychology and robotherapy, approaches that have been developed by the authors. These new fields consist of a concept that places the relationships between humans and robots into a psychological, rather than technological, context. Conceptual and experimental results of implementing the robotic psychology and robotherapy concept into the study of human-robot interactions concern basic operational definitions, theoretical framework, and the design of a unified assessment tool named the Person-Robot Complex Interactive Scale (PRCIS). A study with a robotic cat provides the first results of cross-cultural analysis of person-robot communication, as well as findings on the robot's use by children, young and older adults, and elderly persons with dementia.
Conversion of Carbon Dioxide to Metabolites by &lt;i&gt;Clostridium acetobutylicum&lt;/i&gt; KCTC1037 Cultivated with Electrochemical Reducing Power (2012)

In this research, metabolic fixation of CO2 by growing cells of C. acetobutylicum cultivated with electrochemical reducing power was tested on the basis of the metabolites production and genes expression. In cyclic voltammetry, electrochemical oxidation and reduction reaction of neutral red (NR) immobilized in intact cells of C. acetobutylicum was stationarily repeated like the soluble one in the condition without CO2 but the electrochemical reduction reaction was selectively increased by addition of CO2. In electrochemical bioreactor, the modified graphite felt cathode with NR (NR-cathode) induced C. acetobutylicum to generate acetate, propionate, and butyrate from CO2 in defined medium. When H2 and CO2 were used as an electron donor and an electron acceptor, respectively, C. acetobutylicum also produced the same metabolites in a defined medium. C. acetobutylicum was not grown in the defined medium without substituted electron donors (H2 or electrochemical reducing power). C. acetobutylicum cultivated with electrochemical reducing power produced more butyrate than acetate in complex medium but produced more acetate than butyrate in defined medium. The genes of encoding the enzymes catalyzing acetyl-CoA in C. acetobutylicum electrochemically cultivated in defined medium than conventionally cultivated in complex medium. These results are a clue that C. acetobutylicum may metabolically convert CO2 to metabolites and produce free energy from the electrochemical reducing power.
Molecular Cloning, Nucleotide Sequence, and Expression of the Structural Gene for Alkaline Serine Protease from Alkaliphilic<i>Bacillus</i>sp. 221 (1992)

The gene encoding an alkaline serine protease from alkaliphilic Bacillus sp. 221 was cloned in Escherichia coli and expressed in Bacillus subtilis. An open reading frame of 1,140 bases, identified as the protease gene was preceded by a putative Shine-Dalgarno sequence (AGGAGG) with a spacing of 7 bases. The deduced amino acid sequence had a pre-pro-peptide of 111 residues followed by the mature protease comprising 269 residues. The alkaline protease from alkaliphilic Bacillus sp. 221 had higher homology to the protease from alkaliphilic bacilli (82.1% and 99.6%) than to those from neutrophilic bacilli (60.6-61.7%). Also Bacillus sp. 221 protease and other protease from alkaliphilic bacilli shared common amino acid changes and 4 amino acid deletions that seemed to be related to characteristics of the enzyme of alkaliphilic bacilli when compared to the proteases from neutrophilic bacilli.
Generation of Flavor and Odor Compounds through Fermentation Processes (1986)

Since Neolithic times, microorganisms have been used to achieve desired flavors in foods and beverages. In recent years, a commercial demand has developed for flavoring materials derived from natural sources. Microbiologists and flavorists are exploiting the fermentative action of microorganisms to improve the flavor of alcoholic beverages, cheese, yogurt, bread, fruit, and vegetable products. Many chemicals produced by microbial processes are odorants or tastants. Categories of molecules will be reviewed as a function of substrates and microbial strains. That microorganisms are also capable of de novo synthesis of tastants and odorants from suitable substrates will be illustrated by several examples.
Adriamycin Alopecia Prevented by Cold Air Scalp Cooling (1986)

Preliminary studies are reported on the effectiveness of cold air scalp cooling to prevent alopecia in patients receiving Adriamycin. Cold air produced in a novel way using a vortex refrigeration tube was applied to the scalp for 15 min before and 30 min after the administration of Adriamycin and other cytotoxic agents. Sixteen of 26 patients had no hair loss, four had slight hair loss, and six required a wig. Two subgroups fared particularly well. Four of four patients treated with ABVD for Hodgkin's disease and nine of 13 treated with Adriamycin (40 mg/m2) and vincristine (2 mg) for breast cancer had no hair loss.