gebhart/patrepeval huggingface dataset:
The gene product encoded by ORF 57 of herpesvirus saimiri regulates the redistribution of the splicing factor SC-35. (1999)

The herpesvirus saimiri (HVS) gene product encoded by ORF 57 shares limited C-terminal similarity with herpes simplex virus 1 ICP27, a protein that has been demonstrated to be involved in the inhibition of host-cell splicing and is responsible for the redistribution of components of the spliceosome. It has previously been shown that ORF 57 can either activate or repress viral gene expression by a post-transcriptional mechanism. Furthermore, repression of gene expression by ORF 57 is dependent on the presence of an intron within the target gene coding region. In this report, it is shown that HVS infection results in the redistribution of the SC-35 splicing factor in the infected cell nucleus. Furthermore, the redistributed SC-35 colocalized with the ORF 57 protein product and expression of the protein alone was sufficient to cause the redistribution of the spliceosome components. These results suggest that the mechanism by which ORF 57 down-regulates expression of intron-containing genes involves the redistribution of the spliceosome complex.
Reconfigurable asynchronous logic automata (2010)

Computer science has served to insulate programs and programmers from knowledge of the underlying mechanisms used to manipulate information, however this fiction is increasingly hard to maintain as computing devices decrease in size and systems increase in complexity. Manifestations of these limits appearing in computers include scaling issues in interconnect, dissipation, and coding. Reconfigurable Asynchronous Logic Automata (RALA) is an alternative formulation of computation that seeks to align logical and physical descriptions by exposing rather than hiding this underlying reality. Instead of physical units being represented in computer programs only as abstract symbols, RALA is based on a lattice of cells that asynchronously pass state tokens corresponding to physical resources. We introduce the design of RALA, review its relationships to its many progenitors, and discuss its benefits, implementation, programming, and extensions
Towards Providing Data Validation as a Service (2012)

Data validation is one of the most important and, possibly, most under-valued task in an organization. Without clean data, an organization cannot employ sophisticated analysis and optimization tools to strive for excellence in operations, delivery or planning. Organizations have started to realize the value of data and its impact on their efficiency. Typically, they either develop in-house solutions or purchase industry standard solutions. In this work, we propose an alternative of data validation as a service offering. We argue that such a service would be a profitable proposition for both the parties, provider as well as consumer. We present a general framework to enable such an offering. We provide details on one such implementation that we carried to showcase the viability of such an approach. We propose multiple variants of the offering to handle privacy concerns of the consumer. Finally, we present a set of initial results comparing the different variants.
Linearised remote sensing using a monomode fibre polarimetric sensor (1984)

A remote polarimetric sensor configuration is described, and two signal detection schemes which can be utilised in conjunction with the sensor to provide a linearised output are demonstrated.
Baseline-Free Quantitative Absorption Spectroscopy Based on Molecular Free Induction Decay (2019)

We demonstrate baseline-free, quantitative absorption spectroscopy based on modifications to the free induction decay signal of molecules in the time domain. We apply the technique to a broad-absorbing mixture to retrieve concentrations with unprecedented accuracy. © 2019 The Author(s)
Undesirable combustion characteristic detection apparatus (1984)

Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter Facebook Reddit LinkedIn Tools Icon Tools Reprints and Permissions Cite Icon Cite Search Site Citation Ingo Dudeck, Manfred Maass; Undesirable combustion characteristic detection apparatus. J. Acoust. Soc. Am. 1 August 1984; 76 (2): 654. https://doi.org/10.1121/1.391187 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAcoustical Society of AmericaThe Journal of the Acoustical Society of America Search Advanced Search |Citation Search
Hybrid ray optics and parabolic equation methods for radar propagation modeling (1992)

The use of parabolic equation (PE) methods has become very popular in recent years for modeling radar propagation effects in the lower atmosphere, especially for in which the vertical refractive index profile changes along the propagation path. The PE method normally used is the split-step method described by Tappert, which has been implemented by Dockery, Craig and Levy, and others. An advantage of the PE method is its ability to compute propagation effects within the horizon as well as beyond the horizon, thereby allowing computations to be made in all regions of practical interest to radar engineers or operators with just one model. However, a significant disadvantage of the split-step PE method is that it requires extensive computation. Computational requirements increase with higher frequencies, larger antenna beamwidths, and higher altitudes for which results are desired. For many practical combinations of these parameters, the use of PE models on personal computers is impractical without extra hardware such as transputers.... Propagation assessment, Environmental data, Command and control, Tactical decision aids.
Auto-Scaling Cloud-Based Memory-Intensive Applications (2020)

Today, Cloud providers offer simplistic scaling policies that rely on thresholds that force tenants to have a priori knowledge of their workloads. We develop a new method for scaling memory-intensive workloads that needs no thresholds. This makes it worry-free for tenants, and it adapts even as workloads evolve. This is especially hard for memory-bound applications where even a small decrease in the amount of memory available can have a dramatic, almost unbounded impact on performance. Hence, sizing a machine's physical memory correctly is critical to application performance and operating cost. To determine a natural threshold for memory-intensive applications, our approach automatically analyzes an application's miss ratio curve (MRC) and models it as a hyperbola. Intuitively, a memory scaling policy should operate at the point where the curve flattens: that is, at its intersection with its latus rectum (LR). Our system uses a new approach to constructing and analyzing MRCs at run time that captures memory references from a slice of any scalable application as it executes on standard virtual machines from any major Cloud provider. We demonstrate with multiple applications running on Amazon Web Services (AWS) and Microsoft Azure. Our implementation and evaluation show that, though the LR doesn't require tenants to set thresholds, it is effective in scaling memory-intensive workloads to save on operating costs while avoiding queuing, thrashing, or collapse. It increases throughput by 1.5× and reduces queuing delay by 2× in our evaluation.
Multiplex Lysosomal Enzyme Activity Assay on Dried Blood Spots Using Tandem Mass Spectrometry (2009)

Deficiencies in any of the 50 degradative enzymes found in lysosomes results in the accumulation of undegraded material and subsequently cellular dysfunction. Early identification of deficiencies before irreversible organ and tissue damages occur leads to better clinical outcomes. In the method which follows, lysosomal alpha-glucosidase, alpha-galactosidase, beta-glucocerebrosidase, acid sphingomyelinase, and galactocerebrosidase are extracted from dried blood spots and incubated individually with an enzyme-specific cocktail containing the corresponding substrate and internal standard. Each enzyme cocktail is prepared using commercially available mixture of substrate and internal standard at the predetermined optimized molar ratio. After incubation, the enzymatic reactions are quenched using an ethyl acetate/methanol solution and all five enzyme solutions are combined. The mixtures of the reaction products are prepared using liquid-liquid and solid-phase extractions and quantified simultaneously using selected ion monitoring on LC-MS-MS system.
Throughput estimate of an X-ray projection lithography system (1991)

A recent feasibility study on X-ray projection lithography (XRPL) has demonstrated an extremely high resolution reaching 0.1 µ m 1 ). For practical manufacturing of integrated circuits, however, a high throughput is also necessary. Although the throughput of an XRPL system was already estimated at a wavelength of 4.5nm 2) , this system is difficult to build owing to low performance of actual multilayers. We discuss the throughput of an XRPL system using 13nm radiation for which good quality multilayers can be fabricated 3) .
Novel spirosuccinimides with incorporated isoindolone and benzisothiazole 1,1-dioxide moieties as aldose reductase inhibitors and antihyperglycemic agents (1992)

Compounds from two novel series of spirosuccinimides were prepared. Analogs of series 2 possessed a spiro-fused isoindolone moiety while those of series 3 contained a spiro-fused benzisothiazole S,S-dioxide group. These compounds were evaluated as aldose reductase inhibitors (ARI) in vitro by their ability to inhibit glyceraldehyde reduction using a partially purified bovine lens aldose reductase preparation and in vivo as inhibitors of galactitol accumulation in the lens, sciatic nerve, and diaphragm of galactose-fed rats. Many members from the isoindolone series 2, particularly those containing an isoindolone N-methyl moiety, showed good in vitro and in vivo potency. The most potent member, the 6-chloro analog 32, was resolved, and aldose reductase activity was found to reside almost exclusively in the (+)-enantiomer. Compound 32 was approximately equipotent in the sciatic nerve of the galactose-fed rat to other cyclic imide ARI's of similar in vitro activity, namely sorbinil and ADN-138 and also to tolrestat, an acetic acid-based ARI (ED50's 4-8 mg/kg). Compounds from both series, 2 and 3, were also found to lower plasma glucose levels of genetically obese db/db and ob/ob mice with potency similar to that of ciglitazone. However, members from these series failed to lower insulin levels of the ob/ob mouse at the doses tested.
Direct Measurement of KDM1A Target Engagement Using Chemoprobe-based Immunoassays (2019)

The assessment of the target engagement, defined as the interaction of a drug with the protein it was designed for, is a basic requirement for the interpretation of the biological activity of any compound in drug development or in basic research projects. In epigenetics, target engagement is most often assessed by the analysis of proxy markers instead of measuring the union of the compound to the target. Downstream biological readouts that have been analyzed include the histone mark modulation or gene expression changes. KDM1A is a lysine demethylase that removes methyl groups from mono- and dimethylated H3K4, a modification associated with the silencing of gene expression. Modulation of the proxy markers is dependent on the cell type and function of the genetic make-up of the cells investigated, which can make interpretation and cross-case comparison quite difficult. To circumvent these problems, a versatile protocol is presented to assess the dose effects and dynamics of the direct KDM1A target engagement. The assay described makes use of a KDM1A chemoprobe to capture and quantify uninhibited enzyme, can be broadly applied to cells or tissue samples without the need for genetic modification, has an excellent window of detection, and can be used both for basic research and analysis of clinical samples.
Variable angle, fixed position, beam alignment device (1991)

We describe a general beam alignment device that allows variation of the angle of incidence while preserving the position of incidence on a sample.
Principles of Skeptical Systems (2003)

The high degree of autonomy that is being built into today’s control systems, coupled with their vulnerability to attack at the computer network level, mandates a recasting of the man-machine social contract. We now have the technology and the capability to enable these control systems to entertain “a healthy skepticism ” about the validity of the commands being given then and the identity of the issuers.
Rapid confocal imaging of large areas of excised tissue with strip mosaicing (2011)

Imaging large areas of tissue rapidly and with high resolution may enable rapid pathology at the bedside. The limited field of view of high-resolution microscopes requires the merging of multiple images that are taken sequentially to cover a large area. This merging or mosaicing of images requires long acquisition and processing times, and produces artifacts. To reduce both time and artifacts, we developed a mosaicing method on a confocal microscope that images morphology in large areas of excised tissue with sub-cellular detail. By acquiring image strips with aspect ratios of 10:1 and higher (instead of the standard ~1:1) and "stitching" them in software, our method images 10 × 10 mm(2) area of tissue in about 3 min. This method, which we call "strip mosaicing," is currently three times as fast as our previous method.
An Approach to the Analysis of a Finite Thickness Helix Slow-Wave Structure (2007)

The helical slow-wave structure which has higher frequency and bigger output power is required in the development of high-power traveling wave tube. In view of this point, a thick tape helix SWS is analyzed in this paper. The radial field expression and dispersion equation are derived with the helical coordinate system. The effect of the helix thickness on the dispersion characteristic has been analyzed by numerical calculation. And the theoretical results by our approach have also been compared with those by HooK's method. It shows that the data of our approach is closer to CST simulation. In the paper, we have theoretically modeled a helical SWS by a sheath helix of finite thickness that is treated by utilizing helical coordinate system to account for the finite thickness of the helix wire. The dispersion characteristics and the interaction impendence of the method have been compared with these of Hook's models.
Distinct Fecal and Plasma Metabolites in Children with Autism Spectrum Disorders and Their Modulation after Microbiota Transfer Therapy (2020)

Accumulating evidence has strengthened a link between dysbiotic gut microbiota and autism. Fecal microbiota transplant (FMT) is a promising therapy to repair dysbiotic gut microbiota. We previously performed intensive FMT called microbiota transfer therapy (MTT) for children with autism spectrum disorders (ASD) and observed a substantial improvement of gastrointestinal and behavioral symptoms. We also reported modulation of the gut microbiome toward a healthy one. In this study, we report comprehensive metabolite profiles from plasma and fecal samples of the children who participated in the MTT trial. With 619 plasma metabolites detected, we found that the autism group had distinctive metabolic profiles at baseline. Eight metabolites (nicotinamide riboside, IMP, iminodiacetate, methylsuccinate, galactonate, valylglycine, sarcosine, and leucylglycine) were significantly lower in the ASD group at baseline, while caprylate and heptanoate were significantly higher in the ASD group. MTT drove global shifts in plasma profiles across various metabolic features, including nicotinate/nicotinamide and purine metabolism. In contrast, for 669 fecal metabolites detected, when correcting for multiple hypotheses, no metabolite was significantly different at baseline. Although not statistically significant, <i>p</i>-cresol sulfate was relatively higher in the ASD group at baseline, and after MTT, the levels decreased and were similar to levels in typically developing (TD) controls. <i>p</i>-Cresol sulfate levels were inversely correlated with <i>Desulfovibrio</i>, suggesting a potential role of <i>Desulfovibrio</i> on <i>p</i>-cresol sulfate modulation. Further studies of metabolites in a larger ASD cohort, before and after MTT, are warranted, as well as clinical trials of other therapies to address the metabolic changes which MTT was not able to correct.<b>IMPORTANCE</b> Despite the prevalence of autism and its extensive impact on our society, no U.S. Food and Drug Administration-approved treatment is available for this complex neurobiological disorder. Based on mounting evidences that support a link between autism and the gut microbiome, we previously performed a pioneering open-label clinical trial using intensive fecal microbiota transplant. The therapy significantly improved gastrointestinal and behavioral symptoms. Comprehensive metabolomic measurements in this study showed that children with autism spectrum disorder (ASD) had different levels of many plasma metabolites at baseline compared to those in typically developing children. Microbiota transfer therapy (MTT) had a systemic effect, resulting in substantial changes in plasma metabolites, driving a number of metabolites to be more similar to those from typically developing children. Our results provide evidence that changes in metabolites are one mechanism of the gut-brain connection mediated by the gut microbiota and offer plausible clinical evidence for a promising autism treatment and biomarkers.
Inverted-V chirped phased arrays of gain-guided GaAs/GaAlAs diode lasers (1985)

Phased arrays of diode lasers can produce single-lobed diffraction-limited beams directed normal to the array facet only if they oscillate in the fundamental supermode.1,2 However, most phased arrays demonstrated thus far exhibited double-lobed beams because of their tendency to operate in the higher-order supermodes. Recently, we have shown theoretically that efficient supermode control can be achieved by employing chirped array structures in which the channel phase velocities vary across the array.3 The present work describes the lasing characteristics of inverted-V chirped arrays of GaAs/GaAlAs lasers, which exhibit single-lobed diffraction-limited beams directed normal to the array facet.
Composition‐Dependent Electrocatalytic Activity of Pt‐Cu Nanocube Catalysts for Formic Acid Oxidation (2010)

The die is cast: The electrocatalytic performance of Pt and Pt-Cu nanocubes with different compositions was investigated. Pt80Cu20 nanocubes were found to have high electrocatalytic activity and remarkable long-term stability for formic acid oxidation. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
A complexity-utility framework for modeling decoding complexity towards optimizing subjective quality of video for mobile devices (2010)

In this paper, the complexity of decoding a video clip on a resource constrained mobile device is modeled in terms of content properties and hardware capabilities. It is discussed that the decoding complexity basically depends on specialized hardware that are utilized during the decoding process. A utility-based prediction framework that defines the relationship between content complexity and subjective user satisfaction is presented. Finally, it is argued that such a framework could be utilized to determine efficiently the video coding parameters that will result in maximum user satisfaction and subjective video quality without exceeding the hardware capabilities of a client device.
Identification of Bone Morphogenetic Protein-2 in Early<i>Xenopus laevis</i>Embryos (1992)

Polyclonal antibodies capable of reacting with amphibian bone morphogenetic protein (BMP-2 and -4) were raised in rabbits by immunization with a synthetic 21 amino acid peptide which corresponds to a sequence residing in the mature protein of Xenopus BMP-2 (xBMP-2). The antibodies recognized an embryonic BMP as well as mammalian and bacteria expressed recombinant xBMPs. The antibodies detected, under reducing conditions, a 30 kDa protein in the extract of oocytes and embryos during early development. Interestingly, acidification of the extract from each developmental stage yielded a protein band of smaller molecular weight of 18 kDa, which is similar in size to reduced form of mature BMPs purified from mammalian species. Two-dimensional electrophoresis employed to examine the molecular weight of unreduced forms using the antibody, revealed that both molecular forms are monomeric in the embryos. The result suggests that at least BMP-2 mRNA previously detected in early embryos, is translated into peptide but the dimerization may be incomplete or strictly limited in these embryos.
Molecular Characterization of the Interaction of Staphylococcal Microbial Surface Components Recognizing Adhesive Matrix Molecules (MSCRAMM) ClfA and Fbl with Fibrinogen (2009)

The ligand-binding domain of Fbl (the fibrinogen binding protein from Staphylococcus lugdunensis) shares 60% sequence identity with ClfA (clumping factor A) of Staphylococcus aureus. Recombinant Fbl corresponding to the minimum fibrinogen-binding region (subdomains N2N3) was compared with ClfA for binding to fibrinogen. Fbl and ClfA had very similar affinities for fibrinogen by surface plasmon resonance. The binding site for Fbl in fibrinogen was localized to the extreme C terminus of the fibrinogen gamma-chain at the same site recognized by ClfA. Isothermal titration calorimetry showed that Fbl and ClfA had very similar affinities for a peptide mimicking the C-terminal segment of the fibrinogen gamma-chain. The peptide also inhibited binding of Fbl and ClfA to fibrinogen. A series of substituted gamma-chain variant peptides behaved very similarly when used to inhibit ClfA and Fbl binding to immobilized fibrinogen. Both ClfA and Fbl bound to bovine fibrinogen with a lower affinity compared with human fibrinogen and did not bind detectably to ovine fibrinogen. The structure of the N2N3 subdomains of Fbl in complex with the fibrinogen gamma-chain peptide was modeled based on the crystal structure of the N2N3 subdomains of the ClfA-gamma-chain peptide complex. Residues in the putative binding trench likely to be involved in fibrinogen binding were identified. Fbl variant proteins with alanine substitutions in key residues had reduced affinities for fibrinogen. Thus Fbl and ClfA bind the same site in fibrinogen by similar mechanisms.
Ultra-wideband multiple access: unification and narrowband interference analysis (2003)

Interference from co-existing narrowband services constitutes the bottleneck of ultra-wideband (UWB) communications. To quantify interference and compare UWB systems on that basis, a general framework is needed. In this paper, we develop a unifying transmission and Rake reception model for UWB multiple access encompassing existing and novel spreading codes: direct-sequence (DS), digital single- and multi-carrier (SC/MC), time-hopping (TH), and combinations of them. Performance analysis is also carried out in closed-form. The relative merits of several UWB systems are further assessed by simulations, in narrowband interference (NBI), multipath, and additive white Gaussian noise (AWGN), for both matched-filter (MF), and minimum, mean-square error (MMSE) Rake receivers.