gebhart/patrepeval huggingface dataset:
Stimulation of Reactive Oxygen Species Production and Cytotoxicity in Human Neutrophils <i>in vitro</i> and after Oral Administration of a Polyenzyme Preparation (1995)

Polymorphonuclear neutrophils (PMN) can be primed for enhanced release of reactive oxygen species (ROS) by exposure to cytokines and biological response modifiers. ROS are considered to possess tumoricidal activity. The polyenzyme preparation Wobenzym (WE) contains pancreatin, papain, bromelain trypsin and chymotrypsin and is used in adjuvant tumor therapy. We investigated killing of WE-exposed PMN against tumor cells and analyzed WE influence on ROS production in a chemiluminescence assay in PMN in vitro and in vivo. Depending on dose WE stimulates the cytotoxic capacity of PMN in vitro against tumor cells (50 micrograms/ml:p < 0.01). Exposure of PMN to Wobenzym caused a time-dependent significant (p < 0.02) increase in release of ROS. Similarly, oral administration of Wobenzym to healthy volunteers (n = 28) resulted in significant increases (p < 0.01) in ROS production, depending on dose (peak with 20 tablets) and time (peak 4 hours after Wobenzym administration). In contrast, ROS production was not elevated in the PMN of healthy volunteers receiving placebo (n = 8) or no treatment (n = 16). These findings point to an immunomodulatory capacity of WE in adjuvant tumor therapy.
Control of a Variable-Speed Pitch-Regulated Wind Turbine (2005)

In this report, the speed control of a modern pitch-regulated wind turbine is investigated. A controller structure is derived and the significance of various parameters in the controller structure is investigated.In particular the influence of the speed control bandwidth on the speed variations, torque stresses and energy production is analyzed.The control structure operates without any knowledge of the wind speed or shaft torque, instead these quantities are estimated from the rotor speed and electric power.The results are compared with measurements made on two commercial turbines and the agreement between measurements and simulations is satisfactory
60-GHz Four-Element Phased-Array Transmit/Receive System-in-Package Using Phase Compensation Techniques in 65-nm Flip-Chip CMOS Process (2012)

AThe 60-GHz four-element phased-array transmit/receive (TX/RX) system-in-package antenna modules with phase-compensated techniques in 65-nm CMOS technology are presented. The design is based on the all-RF architecture with 4-bit RF switched LC phase shifters, phase compensated variable gain amplifier (VGA), 4:1 Wilkinson power combining/dividing network, variable-gain low-noise amplifier, power amplifier, 6-bit unary digital-to-analog converter, bias circuit, electrostatic discharge protection, and digital control interface (DCI). The 2 × 2 TX/RX phased arrays have been packaged with four antennas in low-temperature co-fired ceramic modules through flip-chip bonding and underfill process, and phased-array beam steering have been demonstrated. The entire beam-steering functions are digitally controllable, and individual registers are integrated at each front-end to enable beam steering through the DCI. The four-element TX array results in an output of 5 dBm per channel. The four-element RX array results in an average gain of 25 dB per channel. The four-element array consumes 400 mW in TX and 180 mW in RX and occupies an area of 3.74 mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> in the TX integrated circuit (IC) and 4.18 mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> in the RX IC. The beam-steering measurement results show acceptable agreement of the synthesized and measured array pattern.
A methodology for choosing a physician profiling system: the case of First Option Health Plan. (1999)

The public continues to demand improved health care at affordable costs. It appears that this public pressure will force health maintenance organizations (HMOs) and other health care providers to face their toughest days in the years to come. They will need to find solutions to resolve this "quality and cost" problem. One method that is likely to have the ability to improve quality of care and control costs is physician profiling. That is, data related to a physician's care or outcomes can be collected and compared with profiles of various populations, for example, other similar providers in a relatively close geographical proximity. This article examines the issues involved in physician profiling, including the characteristics of profiling systems and the direct and indirect benefits of having such a system. Further, the article uses an actual HMO example to discuss considerations that administrators should make when choosing a physician profiling system.
Real-Time Deep Learning at the Edge for Scalable Reliability Modeling of Si-MOSFET Power Electronics Converters (2019)

With the significant growth of advanced high-frequency power converters, online monitoring and active reliability assessment of power electronic devices are extremely crucial. This paper presents a transformative approach, named deep learning reliability awareness of converters at the edge (Deep RACE), for real-time reliability modeling and prediction of high-frequency MOSFET power electronic converters. Deep RACE offers a holistic solution which comprises algorithm advances, and full system integration (from the cloud down to the edge node) to create a near real-time reliability awareness. On the algorithm side, this paper proposes a deep learning algorithmic solution based on stacked long short-term memory for collective reliability training and inference across collective MOSFET converters based on device resistance changes. Deep RACE also proposes an integrative edge-to-cloud solution to offer a scalable decentralized devices-specific reliability monitoring, awareness, and modeling. The MOSFET convertors are Internet-of-Things (IoT) devices which have been empowered with edge real-time deep learning processing capabilities. The proposed Deep RACE solution has been prototyped and implemented through learning from MOSFET data set provided by NASA. Our experimental results show an average miss prediction of 8.9% over five different devices which is a much higher accuracy compared to well-known classical approaches (Kalman filter and particle filter). Deep RACE only requires 26-ms processing time and 1.87-W computing power on edge IoT device.
Nonalloyed ohmic contacts to heavily Be-doped GaP and In<i>x</i>Ga1−<i>x</i>P (1996)

Nonalloyed ohmic contacts, with contact resistances as low as 9 μΩ cm2, are obtained to p++ GaP using Ni and Ti–Au. Very high p-type doping (5×1019 cm−3) is achieved in GaP using a growth temperature of 400 °C followed by an ex situ high-temperature rapid thermal anneal. The p-type dopant is beryllium and the films are grown by solid source molecular beam epitaxy equipped with a valved phosphorus cracker. A record high hole concentration (2×1019 cm−3) is reported in In0.49Ga0.51P by using a growth temperature of 350 °C.
Anatomical Characteristics of Artificially Induced Tension Wood in Seedlings of Honduras Mahogany (2006)

Seedlings of Honduras mahogany (Swietenia macrophylla King) were laid horizontally for 4 and 10 mo to induce reaction wood. Gelatinous fibers occurred on the upper side of the trunks of the treated seedlings, and the longer the treatment, the higher the extent of the gelatinous fibers. More-abundant axial parenchymatous cells and starch grains were observed in the opposite wood than in the reaction wood. Such anatomical characteristics suggest that there are distinct patterns of regulation between the cambium zones of the reaction wood and opposite wood. Starch storage in axial parenchymatous cells may play a role in the active or passive processes involved in the biochemical regulation and source-to-sink transportation.
Relationship between physical and chemical properties of aluminum-containing adjuvants and immunopotentiation (2007)

Aluminum-containing adjuvants are an important component of many vaccines because they safely potentiate the immune response. The structure and properties of aluminum hydroxide adjuvant, aluminum phosphate adjuvant and alum-precipitated adjuvants are presented in this review. The major antigen adsorption mechanisms, electrostatic attraction and ligand exchange, are related to the adjuvant structure. The manner by which aluminum-containing adjuvants potentiate the immune response is related to the structure, properties of the adjuvant and adsorption mechanism. Immunopotentiation occurs through the following sequential steps: inflammation and recruitment of antigen-presenting cells, retention of antigen at the injection site, uptake of antigen, dendritic cell maturation, T-cell activation and T-cell differentiation.
Artificial Polychromatic Light Affects Growth and Physiology in Chicks (2014)

Despite the overwhelming use of artificial light on captive animals, its effect on those animals has rarely been studied experimentally. Housing animals in controlled light conditions is useful for assessing the effects of light. The chicken is one of the best-studied animals in artificial light experiments, and here, we evaluate the effect of polychromatic light with various green and blue components on the growth and physiology in chicks. The results indicate that green-blue dual light has two side-effects on chick body mass, depending on the various green to blue ratios. Green-blue dual light with depleted and medium blue component decreased body mass, whereas enriched blue component promoted body mass in chicks compared with monochromatic green- or blue spectra-treated chicks. Moreover, progressive changes in the green to blue ratios of green-blue dual light could give rise to consistent progressive changes in body mass, as suggested by polychromatic light with higher blue component resulting in higher body mass. Correlation analysis confirmed that food intake was positively correlated with final body mass in chicks (R2 = 0.7664, P = 0.0001), suggesting that increased food intake contributed to the increased body mass in chicks exposed to higher blue component. We also found that chicks exposed to higher blue component exhibited higher blood glucose levels. Furthermore, the glucose level was positively related to the final body mass (R2 = 0.6406, P = 0.0001) and food intake (R2 = 0.784, P = 0.0001). These results demonstrate that spectral composition plays a crucial role in affecting growth and physiology in chicks. Moreover, consistent changes in spectral components might cause the synchronous response of growth and physiology.
Analytical Theory of Combined Condensing/Vaporizing Gas Drives (1993)

SUMMARY Analytical solutions are presented that confirm the existence of a combined condensing and vaporizing displacement mechanism in enriched gas drives. The solutions are derived for dispersion-free, one-dimensional displacements in four-component hydrocarbon systems. A simple geometric construction is used to find a key tie line in the solution, the "crossover" tie line. This tie line is shown to control the development of miscibility in condensing/vaporizing systems, and it connects the condensing and vaporizing portions of the displacement.
Electromagnetic Propulsion of Matter in Violation of Newton's 3 rd Law (2010)

The intention of this article to hypothesize a theory of electromagnetic propulsion of matter, using a pulsed electric current, located within a wire conductor that induces a magnetic field, which then interacts with its own current to propel that conductor [Mass or matter] without a propellant. This function is a violation of Newton’s 3rd Law. So in this scenario F ma  in one direction does not equal F ma  in the opposite direction.
Altered expression of miR-152 and miR-148a in ovarian cancer is related to cell proliferation (2011)

microRNAs (miRs) are endogenous small non-coding RNAs that are aberrantly expressed in various carcinomas. miR-152 and miR-148a have not been comprehensively investigated in ovarian cancer. Thus, the aim of this study was to identify the role of miR-152 and miR-148a in epithelial ovarian cancer. Total RNA was extracted from tissues of 78 patients with epithelial ovarian cancer, 17 normal ovarian epithelium tissues and two ovarian cancer cell lines. Using quantitative real-time PCR (qRT-PCR) followed by the 2-ΔΔCT method for calculating the results, we found that the expression levels of miR-152 were significantly decreased in ovarian cancer tissues compared to normal ovarian epithelium tissues (p<0.05). However, although the expression of miR-148a was also decreased in 65% of patients, no statistically significant difference in expression was found. A strong correlation was found between the expression of miR-152 and miR-148a (p<0.001, Pearson's correlation). The relationship between miR-152 or miR-148a expression levels in ovarian cancer and clinicopathological features, response to therapy and short-term survival was analyzed and the results showed that no correlation existed. In addition, we found that both miR-152 and miR-148a were down-regulated in ovarian cancer cell lines. After miR-152 or miR-148a mimics were transfected into ovarian cancer cell lines, the MTT cell proliferation assay showed that cell proliferation was significantly inhibited. Taken together, miR-152 and miR-148a may be involved in the carcinogenesis of ovarian cancer through deregulation of cell proliferation. They may be novel biomarkers for early detection or therapeutic targets of ovarian cancer.
Energy Cooperation in Energy Harvesting Communications (2013)

In energy harvesting communications, users transmit messages using energy harvested from nature during the course of communication. With an optimum transmit policy, the performance of the system depends only on the energy arrival profiles. In this paper, we introduce the concept of energy cooperation, where a user wirelessly transmits a portion of its energy to another energy harvesting user. This enables shaping and optimization of the energy arrivals at the energy-receiving node, and improves the overall system performance, despite the loss incurred in energy transfer. We consider several basic multi-user network structures with energy harvesting and wireless energy transfer capabilities: relay channel, two-way channel and multiple access channel. We determine energy management policies that maximize the system throughput within a given duration using a Lagrangian formulation and the resulting KKT optimality conditions. We develop a two-dimensional directional water-filling algorithm which optimally controls the flow of harvested energy in two dimensions: in time (from past to future) and among users (from energy-transferring to energy-receiving) and show that a generalized version of this algorithm achieves the boundary of the capacity region of the two-way channel.
Modulating Effect of Nigella sativa on Renal Structural Changes by Monosodium Glutamate in Female Mice (2013)

There is considerable evidence, suggest that, consumption of food additives monosodium glutamate (MSG), places humans at risk and the greatest risk is faced by children. The present work aimed for explaining the risks of excessive use of recent food additives MSGasa flavor enhancer and to study the role of black seeds extract Nigellia sativaas a natural and safe product in modulating effect of renal structural changes in female mice. Eighty female Swiss albino mice Musmusculus (2 months old, 20-25 g) divided into 4 groups (20 females each). The first group was used as control one (given distilled water only without MSG and/ or NS). The second group, (given orally a dose of 8 mg MSG/kg of body weight in distilled water). The third group, (given orally a dose of 180 mg powder NS/kg of body weight suspended in distilled water )and the fourth group, (given orally a dose of 8 mg MSG and 180 mg powder NS/kg of body weight in distilled water). Mice were sacrificed at the end of 10 weeks to remove kidneys for histological and histochemical studies. Histological and histochemical investigations of kidney of MSG group showed several changes include dilation and congestion of some blood vessels, certain cells of convoluted tubules affected comprising cellular swelling, hydropic changes, necrosis, enlarged dilation of several convoluted tubules. Investigation also showed infiltration of large number of inflammatory lymphocytes and abnormality in the pattern of kidney tissues and distortion of some glomeruluithat appeared enlarged or lobulated and sometimes congested with red blood cells. Also increased intensity of collagen fibers, proteins and lipids with the general lack of glycogen granules was observed as compared with controls one. The result of this work showed the role of Nigellia sativa extract as a natural and safe product in modulating renal structural changes in female Swiss albino mice treated with MSG and explained the risks of excessive use of MSGas a flavor enhancer.
CD300lf is the primary physiologic receptor of murine norovirus but not human norovirus (2020)

Murine norovirus (MNoV) is an important model of human norovirus (HNoV) and mucosal virus infection more broadly. Viral receptor utilization is a major determinant of cell tropism, host range, and pathogenesis. The bona fide receptor for HNoV is unknown. Recently, we identified CD300lf as a proteinaceous receptor for MNoV. Interestingly, its paralogue CD300ld was also sufficient for MNoV infection in vitro. Here we explored whether CD300lf is the sole physiologic receptor in vivo and whether HNoV can use a CD300 ortholog as an entry receptor. We report that both CD300ld and CD300lf are sufficient for infection by diverse MNoV strains in vitro. We further demonstrate that CD300lf is essential for both oral and parenteral MNoV infection and to elicit anti-MNoV humoral responses in vivo. In mice deficient in STAT1 signaling, CD300lf is required for MNoV-induced lethality. Finally, we demonstrate that human CD300lf (huCD300lf) is not essential for HNoV infection, nor does huCD300lf inhibit binding of HNoV virus-like particles to glycans. Thus, we report huCD300lf is not a receptor for HNoV.
Filamin B Regulates Chondrocyte Proliferation and Differentiation through Cdk1 Signaling (2014)

Humans who harbor loss of function mutations in the actin-associated filamin B (FLNB) gene develop spondylocarpotarsal syndrome (SCT), a disorder characterized by dwarfism (delayed bone formation) and premature fusion of the vertebral, carpal and tarsal bones (premature differentiation). To better understand the cellular and molecular mechanisms governing these seemingly divergent processes, we generated and characterized FlnB knockdown ATDC5 cell lines. We found that FlnB knockdown led to reduced proliferation and enhanced differentiation in chondrocytes. Within the shortened growth plate of postnatal FlnB(-/-) mice long bone, we observed a similarly progressive decline in the number of rapidly proliferating chondrocytes and premature differentiation characterized by an enlarged prehypertrophic zone, a widened Col2a1(+)/Col10a1(+) overlapping region, but relatively reduced hypertrophic zone length. The reduced chondrocyte proliferation and premature differentiation were, in part, attributable to enhanced G2/M phase progression, where fewer FlnB deficient ATDC5 chondrocytes resided in the G2/M phase of the cell cycle. FlnB loss reduced Cdk1 phosphorylation (an inhibitor of G2/M phase progression) and Cdk1 inhibition in chondrocytes mimicked the null FlnB, premature differentiation phenotype, through a β1-integrin receptor- Pi3k/Akt (a key regulator of chondrocyte differentiation) mediated pathway. In this context, the early prehypertrophic differentiation provides an explanation for the premature differentiation seen in this disorder, whereas the progressive decline in proliferating chondrocytes would ultimately lead to reduced chondrocyte production and shortened bone length. These findings begin to define a role for filamin proteins in directing both cell proliferation and differentiation through indirect regulation of cell cycle associated proteins.
Development of a Cold-Adapted Pseudoalteromonas Expression System for the Pseudoalteromonas Proteins Intractable for the Escherichia coli System (2015)

Although the Escherichia coli expression system is the most commonly used expression system, some proteins are still difficult to be expressed by this system, such as proteins with high thermolability and enzymes that cannot mature by autoprocessing. Therefore, it is necessary to develop alternative expression systems. In this study, a cold-adapted Pseudoalteromonas expression system was developed. A shuttle vector was constructed, and a conjugational transfer system between E. coli and psychrophilic strain Pseudoalteromonas sp. SM20429 was established. Based on the shuttle vector, three reporter vectors were constructed to compare the strength of the cloned promoters at low temperature. The promoter of xylanase gene from Pseudoalteromonas sp. BSi20429 was chosen due to its high activity at 10-15°C. An expression vector pEV containing the chosen promoter, multiple cloning sites and a His tag was constructed for protein expression and purification. With pEV as expression vector and SM20429 as the host, a cold-adapted protease, pseudoalterin, which cannot be maturely expressed in E. coli, was successfully expressed as an active extracellular enzyme when induced by 2% oat spelt xylan at 15°C for 48 h. Recombinant pseudoalterin purified from the culture by Ni affinity chromatography had identical N-terminal sequence, similar molecular mass and substrate specificity as the native pseudoalterin. In addition, another two cold-adapted enzymes were also successfully expressed by this system. Our results indicate that this cold-adapted Pseudoalteromonas expression system will provide an alternative choice for protein expression, especially for the Pseudoalteromonas proteins intractable for the E. coli system.
A simple combined microdissection and aspiration device for the rapid procurement of single cells from clinical peripheral blood smears (1998)

Molecular analysis of cells from cytology specimens can help to establish a diagnosis in ambiguous cases. However, mutations in heterogeneous samples might not be detected because of the diluting effect of DNA from normal background cells. Even if a mutation were detected, it could not be traced back to a specific cell type. Molecular analysis of single cells circumvents this problem. Both mechanical and laser assisted methods have been described for the selective procurement of cells from histology slides; however, they have the drawback of either being technically demanding or expensive. Furthermore, it is nuclear whether they can be applied to cytology specimens. Finally, few of these techniques are able to procure single cells. Therefore, we developed a simplified combined microdissection and aspiration device for the rapid procurement of single cells from clinical cytology specimens. The principle of this device, called the cytopicker, is the combination of the microdissection tool, a steel cannula, with the aspiration tool, a glass capillary connected to a vacuum, into one device. Steel cannulae are optimal for microdissection of cells from the hard matrix of cytology specimens but aspirate poorly. On the other hand, glass capillaries are suboptimal for dissecting but aspirate very well. Combining both tools into one by inserting the capillary into the cannula allows optimal dissection using the cannula (with the glass capillary with-drawn and thus protected), followed by optimal aspiration using the capillary (after being advanced through the cannula). All movements of the device are controlled by just one micromanipulator, making the cytopicker inexpensive to manufacture. The cytopicker can rapidly and simply procure single cells, such as lymphoblasts, from cytology specimens, such as peripheral blood smears. DNA from these cells can be amplified by PCR. However, precautions have to be taken to avoid contamination. Once improved further, the cytopicker might facilitate molecular analysis in the routine cytology laboratory.