gebhart/patrepeval huggingface dataset:
Hydrogen Oxidation and Evolution Reaction Kinetics on Carbon Supported Pt, Ir, Rh, and Pd Electrocatalysts in Acidic Media (2014)

The hydrogen oxidation and evolution reaction (HOR/HER) behavior of carbon supported metal (Pt, Ir, Rh, Pd) nanoparticle electrocatalysts is studied using the H 2 pump approach, in a proton exchange membrane fuel cell (PEMFC) setup. After describing the best method for normalizing the net faradaic currents to the active surface area of the electrodes, we measure the HOR/HER kinetic parameters (exchange current densities and transfer coefficients) in a temperature range from 313 K to 353 K and calculate the activation energy for the HOR/HER process. We compare the measured kinetic parameters with those extracted from different mass-transport limitation free setups in literature, to evaluate the hydrogen electrocatalysis on these most active surfaces. The HOR/HER activity scales with the following: Pt > Ir Rh > Pd. The anodic and cathodic transfer coefficients are similar for all metals (ca. 0.5), leading to Tafel slopes of ca. 140 mV/decade at 353 K (except for the anodic branch of Pd and the cathodic branch of Rh). The lowest activation energies are found for Pt and Ir (20 kJ/mol). For Rh and Pd, higher activation energies are found (30 kJ/mol), and attributed to the formation of surface oxides and hydride phase, respectively.
Identification of a physiological E2 module for the human anaphase-promoting complex (2009)

Ubiquitination by the anaphase-promoting complex (APC/C) is essential for proliferation in all eukaryotes. The human APC/C promotes the degradation of mitotic regulators by assembling K11-linked ubiquitin chains, the formation of which is initiated by its E2 UbcH10. Here, we identify the conserved Ube2S as a K11-specific chain elongating E2 for human and Drosophila APC/C. Ube2S depends on the cell cycle-dependent association with the APC/C activators Cdc20 and Cdh1 for its activity. While depletion of Ube2S already inhibits APC/C in cells, the loss of the complete UbcH10/Ube2S-module leads to dramatic stabilization of APC/C substrates, severe spindle defects, and a strong mitotic delay. Ube2S and UbcH10 are tightly co-regulated in the cell cycle by APC/C-dependent degradation. We conclude that UbcH10 and Ube2S constitute a physiological E2-module for APC/C, the activity of which is required for spindle assembly and cell division.
Poliovirus pathogenesis in a new poliovirus receptor transgenic mouse model: age-dependent paralysis and a mucosal route of infection (2002)

We constructed a poliovirus receptor (PVR) transgenic mouse line carrying a PVR delta cDNA driven by a beta-actin promoter. We refer to this model as the cPVR mouse. The cPVR mice express Pvr in a variety of tissues (including small intestines, brain, spinal cord, muscle, blood and liver) and are susceptible to infection after intraperitoneal, intracerebral or intramuscular inoculation of poliovirus. After intraperitoneal inoculation, poliovirus replication is observed in cPVR muscle, brain, spinal cord and, notably, small intestine. The cPVR mice exhibit a striking age-dependent paralysis after intramuscular infection, with 2-week-old mice being 10,000-fold more susceptible to paralytic disease than adult mice. The cPVR mice are also susceptible to paralysis following intranasal infection with poliovirus. After intranasal infection, virus replication is observed in the olfactory bulb, cerebrum, brain stem and spinal cord, suggesting that intranasal infection of cPVR mice is a model for bulbar paralysis. Intranasally infected mice frequently display unusual neurological behaviours. The PVR transgenic mouse reported here provides the first available model for a mucosal route of infection with poliovirus.
Binding of Alphaherpesvirus Glycoprotein H to Surface α<sub>4</sub>β<sub>1</sub>-Integrins Activates Calcium-Signaling Pathways and Induces Phosphatidylserine Exposure on the Plasma Membrane (2015)

Herpesviruses are a large family of enveloped viruses that infect a wide range of hosts, causing a variety of diseases. These viruses have developed a number of strategies for successful entry into different cell types. We and others have shown that alphaherpesviruses, including EHV-1 and herpes simplex virus 1 (HSV-1), can route their entry pathway and do so by manipulation of cell signaling cascades to ensure viral genome delivery to nuclei. We show here that the interaction between EHV-1 gH and cellular α4β1-integrins is necessary to induce emptying of ER calcium stores, which induces phosphatidylserine exposure on the plasma membrane through a scramblase-dependent mechanism. This change in lipid asymmetry facilitates virus entry and might help fusion of the viral envelope at the plasma membrane. These findings will help to advance our understanding of herpesvirus entry mechanism and may facilitate the development of novel drugs that can be implemented for prevention of infection and disease.
A non‐recursive method for solving the general discrete‐time riccati equations related to the <i>H</i><sub>∞</sub> control problem (1994)

Abstract In this paper we propose a nonrecursive method for solving the general discrete‐time algebraic Riccati equation related to the H ∞ control problem ( H ∞ ‐DARE). We have achieved this by casting the problem of solving a given H ∞ ‐DARE to the problem of solving an auxiliary continuous‐time algebraic Riccati equation associated with the H ∞ control problem ( H ∞ ‐CARE) for which the well known nonrecursive methods of solving are available. The advantages of our approach are: it reduces the computation involved in the recursive algorithms while giving much more accurate solutions, and it readily provides the properties of the general H ∞ ‐DARE.
Nonactin biosynthesis: the potential nonactin biosynthesis gene cluster contains type II polyketide synthase-like genes (2000)

Nonactin is the parent compound of a group of highly atypical polyketide metabolites produced by Streptomyces griseus subsp. griseus ETH A7796. In this paper we describe the isolation, sequencing, and analysis of 15¿ omitted¿559 bp of chromosomal DNA, containing the potential nonactin biosynthesis gene cluster, from S. griseus subsp. griseus ETH A7796. Fourteen open reading frames were observed in the DNA sequence. Significantly, type II polyketide synthase (PKS) homologues were discovered in an apparent operon structure, which also contained the nonactate synthase gene (nonS), clustered with the tetranactin resistance gene. The deduced products of two of the genes (nonK and nonJ) are quite unusual ketoacyl synthase (KAS) alpha and KASbeta homologues. We speculate that nonactic acid, the polyketide precursor of nonactin, is synthesized by a type II PKS system.
Hardware-Based Activation Function-Core for Neural Network Implementations (2021)

Today, embedded systems (ES) tend towards miniaturization and the carrying out of complex tasks in applications such as the Internet of Things, medical systems, telecommunications, among others. Currently, ES structures based on artificial intelligence using hardware neural networks (HNNs) are becoming more common. In the design of HNN, the activation function (AF) requires special attention due to its impact on the HNN performance. Therefore, implementing activation functions (AFs) with good performance, low power consumption, and reduced hardware resources is critical for HNNs. In light of this, this paper presents a hardware-based activation function-core (AFC) to implement an HNN. In addition, this work shows a design framework for the AFC that applies a piecewise polynomial approximation (PPA) technique. The designed AFC has a reconfigurable architecture with a wordlength-efficient decoder, i.e., reduced hardware resources are used to satisfy the desired accuracy. Experimental results show a better performance of the proposed AFC in terms of hardware resources and power consumption when it is compared with state of the art implementations. Finally, two case studies were implemented to corroborate the AFC performance in widely used ANN applications.
JUN siRNA regulates matrix metalloproteinase-2 expression, microvascular endothelial growth and retinal neovascularisation (2006)

Transcription factors link changes in the extracellular environment with alterations in gene expression. As such, these molecules serve as attractive targets for intervention in pathological settings. Since JUN has been linked with microvascular disease in humans, we hypothesised that small interfering RNA (siRNA) targeting this immediate-early gene may be useful agents that suppress endothelial growth and neovascularisation. Here we show that Jun siRNA inhibits Jun mRNA and protein expression in murine microvascular endothelial cells, blocks cell proliferation and suppresses migration in a scratch-wound assay. It also inhibits three-dimensional tubular formation on basement membrane extracts and reduces angiogenesis in mice bearing Matrigel plugs as subcutaneous implants. Single intravitreal administration of Jun siRNA reduces neovascularisation in a murine model of proliferative retinopathy, and suppresses endothelial JUN and matrix metalloproteinase-2 (MMP-2) immunoreactivity in retinal vessels, data supported by its repression of MMP-2 expression and gelatinolytic activity in vitro. Co-administration of TGFbeta with the siRNA reverses this neovascular inhibitory effect, which is in turn abrogated by cis-9-octadecenoyl-N-hydroxylamide, consistent with the involvement of a metalloproteinase such as MMP-2. Thus, JUN siRNA can serve as a specific inhibitor of aberrant endothelial and neovascular growth.
A real-time 64-monosyllable recognition LSI with learning mechanism (2001)

In the paper, a real-time 64-mono-syllable recognition LSI is presented. The LSI accepts 11.6 msec speech frame and outputs a 6-bit symbol-code for each frame by the end of the next frame with the pipelining manner. The recognition method is based on the Hidden Markov Model and is speaker-independent. An on-chip learning mechanism has also been designed, but the circuit is off-chip at present implementation because of the restriction of LSI area. The LSI is fabricated by VDEC Rohm with 0.6 um process on a 4.5 mm x 4.5 mm chip.
[2,5-diaryl-substituted 1,3,4-oxadiazoles: synthesis and preliminary pharmacological investigation]. (1984)

Seven 2,5-diarylalkyloxysubstituted 1,3,4-oxadiazoles were synthesized. They showed pronounced antiphlogistic action together with central activity (sedative, analgesic) that principally seems to be connected with the presence in the molecule of the 3,4-dioxymethylenephenyl radical.
Expression of molecular clones of v-myb in avian and mammalian cells independently of transformation (1986)

We demonstrated that molecular clones of the v-myb oncogene of avian myeloblastosis virus (AMV) can direct the synthesis of p48v-myb both in avian and mammalian cells which are not targets for transformation by AMV. To accomplish this, we constructed dominantly selectable avian leukosis virus derivatives which efficiently coexpress the protein products of the Tn5 neo gene and the v-myb oncogene. The use of chemically transformed QT6 quail cells for proviral DNA transfection or retroviral infection, followed by G418 selection, allowed the generation of cell lines which continuously produce both undeleted infectious neo-myb viral stocks and p48v-myb. The presence of a simian virus 40 origin of replication in the proviral plasmids also permitted high-level transient expression of p48v-myb in simian COS cells without intervening cycles of potentially mutagenic retroviral replication. These experiments establish that the previously reported DNA sequence of v-myb does in fact encode p48v-myb, the transforming protein of AMV.
Induction of delayed-type hypersensitivity with BCG-associated proteins. (1976)

Microgram quantities of bovine serum albumin (BSA) and hen egg albumin (OA) associate spontaneously with living BCG in aqueous suspension. The association is stable insofar as bound protein cannot be readily dissociated from the organism by repeated washing. Association of BSA or OA is dependent upon the concentration of specific protein or competing protein in the incubation mixture, pH and the amount of protein already bound to the organisms. Washed BCG "complexes" containing either BSA or OA are potent inducers of delayed-type hypersensitivity (DTH). The same soluble proteins are far less effective inducers of DTH, even when injected with BCG. The capacity of OA-BCG complexes to provoke a cell-mediated response seems to be related, at least in part, to "stabilization" of the antigen on an appropriate carrier and its concentration at the site of BCG injection.
Double-Hit Gene Expression Signature Defines a Distinct Subgroup of Germinal Center B-Cell-Like Diffuse Large B-Cell Lymphoma (2018)

We have defined a clinically and biologically distinct subgroup of tumors within GCB-DLBCL characterized by a gene expression signature of HGBL-DH/TH- BCL2. This knowledge has been translated into an assay applicable to routinely available biopsy samples, which enables exploration of its utility to guide patient management.
Protocol and cell responses in three-dimensional conductive collagen gel scaffolds with conductive polymer nanofibres for tissue regeneration (2013)

It has been established that nerves and skeletal muscles respond and communicate via electrical signals. In regenerative medicine, there is current emphasis on using conductive nanomaterials to enhance electrical conduction through tissue-engineered scaffolds to increase cell differentiation and tissue regeneration. We investigated the role of chemically synthesized polyaniline (PANI) and poly(3,4-ethylenedioxythiophene) (PEDOT) conductive polymer nanofibres for conductive gels. To mimic a naturally derived extracellular matrix for cell growth, type I collagen gels were reconstituted with conductive polymer nanofibres and cells. Cell viability and proliferation of PC-12 cells and human skeletal muscle cells on these three-dimensional conductive collagen gels were evaluated in vitro. PANI and PEDOT nanofibres were found to be cytocompatible with both cell types and the best results (i.e. cell growth and gel electrical conductivity) were obtained with a low concentration (0.5 wt%) of PANI. After 7 days of culture in the conductive gels, the densities of both cell types were similar and comparable to collagen positive controls. Moreover, PC-12 cells were found to differentiate in the conductive hydrogels without the addition of nerve growth factor or electrical stimulation better than collagen control. Importantly, electrical conductivity of the three-dimensional gel scaffolds increased by more than 400% compared with control. The increased conductivity and injectability of the cell-laden collagen gels to injury sites in order to create an electrically conductive extracellular matrix makes these biomaterials very conducive for the regeneration of tissues.
Cognitive Random Stepped Frequency Radar with Sparse Recovery (2013)

Random stepped frequency (RSF) radar, which transmits random-frequency pulses, can suppress the range ambiguity, improve convert detection, and possess excellent electronic counter-countermeasures (ECCM) ability [1]. In this paper, we apply a sparse recovery method to estimate the range and Doppler of targets. We also propose a cognitive mechanism for RSF radar to further enhance the performance of the sparse recovery method. The carrier frequencies of transmitted pulses are adaptively designed in response to the observed circumstance. We investigate the criterion to design carrier frequencies, and efficient methods are then devised. Simulation results demonstrate that the adaptive frequency-design mechanism significantly improves the performance of target reconstruction in comparison with the non-adaptive mechanism.
Statistical theory of multimode random lasers (2005)

We present a semiclassical laser theory for multimode lasing in optical resonators with overlapping modes. Nonlinear saturation and mode competition are characterized in terms of left and right eigenmodes of a non-Hermitian operator. The theory is applied to wave-chaotic cavities and weakly disordered random media. In the limit of sufficiently strong pumping, we find that the mean number of laser peaks increases with the 1/3 power of the pump strength.
Patchmatch-Based Robust Stereo Matching Under Radiometric Changes (2018)

In the real world, the two challenges of stereo vision system include a robust system under various radiometric changes and real-time process. To extract depth information from stereoscopic images, this paper proposes Patchmatch-based robust and fast stereo matching under radiometric changes. For this, a cost function was designed and minimized for estimating an accurate disparity map. Specifically, we used a prior probability to minimize the occlusion region and a smoothness term that considers convexity of objects to extract a fine disparity map. For evaluating the performance of the proposed scheme, we used Middlebury stereo data sets with radiometric changes. The experimental result showed that the proposed method outperforms state-of-the-art methods by up to 3.35 percent better and a range of 4.71 - 27.24 times faster result in terms of bad pixel error and processing time, respectively. Therefore, we believe that the proposed scheme can be a useful tool for computer vision-based applications.
The importance of accurate VR head registration on skilled motor performance (2006)

Many virtual reality (VR) researchers consider exact head registration (HR) and an exact multi-sensory alignment between real world and virtual objects to be a critical factor for effective motor performance in VR. Calibration procedures, however, can be error prone, time consuming and sometimes impractical to perform. To better understand the relationship between head registration and fine motor performance, we conducted a series of reciprocal tapping tasks under four conditions: real world tapping, VR with correct HR, VR with mildly perturbed HR, and VR with highly perturbed HR. As might be expected, VR performance was worse than real world performance. There was no effect of HR perturbation on motor performance in the tapping tasks. We believe that sensorimotor adaptation enabled subjects to perform equally well in the three VR conditions despite the incorrect head registration in two of the conditions. This suggests that exact head registration may not be as critically important as previously thought, and that extensive per-user calibration procedures may not be necessary for some VR tasks.
LES LPC ET LA NORMALISATION (1990)

L'auteur presente les formes d'activite du reseau des Laboratoires des Ponts et Chaussees dans le domaine de la normalisation francaise et europeenne. Il indique la repartition des travaux programmes pour 1990 au sein decommissions de normalisation constituees recemment et animees par le Bureau de Normalisation des Sols et Routes (BNSR) ou de commissions deja anciennes, travaux qui relevent principalement de quatre secteurs techniques : 1-sols, geotechnique et granulats -2-Chaussees -3-Equipements de la route -4-Ouvrages d'art. Pour chaque secteurs des details sont donnes sur les commissions, leur presidence, leur secretariat, leur activite.
Newton-like extremum-seeking part I: Theory (2009)

In practice, the convergence rate and stability of perturbation based extremum-seeking (ES) schemes can be very sensitive to the curvature of the plant map. This sensitivity arises from the use of a gradient descent adaptation algorithm. Such ES schemes may need to be conservatively tuned in order to maintain stability over a wide range of operating conditions, resulting in slower optimisation than could be achieved for a fixed operating condition. This can severely reduce the effectiveness of perturbation based ES schemes in some applications. It is proposed that by using a Newton-like step instead of a more typical gradient descent adaptation law, then the behaviour of the ES scheme near an extremum will be independent of the plant map curvature. In this paper, such a Newton-like ES scheme is developed and its stability and convergence properties are explored.
Deploying a Wireless Downhole Reservoir Testing System Enabling Real-Time Interpretation and Tool Control (2011)

Abstract Successful exploration and field development requires knowledge of fundamental reservoir and production properties. These properties can only be confidently measured by conducting a reservoir test, properly designed to measure pressure, temperature, and flow rate and sample for the zone of interest. Alongside the key task of gathering the necessary data and validating test objectives, this process involves the flowing of hydrocarbons through temporary equipment. It is therefore critical, to safely and efficiently manage the wellbore. A new wireless downhole reservoir testing system has been deployed in Saudi Arabia to meet reservoir testing challenges. This integrated system allows retrieval of downhole measurements throughout the test, control and verification of the tester and circulating valves, and selective activation of multizone perforating guns. This paper presents field cases demonstrating how the system has been used for these applications in an effort to reduce operational risks and improve certainty in reservoir testing results.
Increase of Reaction Rate and Sensitivity of Low-Abundance Enzyme Assay Using Micro/Nanofluidic Preconcentration Chip (2008)

We report a novel method of increasing both the reaction rate and the sensitivity of low-abundance enzyme assay using a micro/nanofluidic preconcentration chip. The disposable preconcentration device made out of PDMS with a surface-patterned ion-selective membrane increases local enzyme/substrate concentrations for rapid monitoring of enzyme activity. As a model system, we used trypsin as the enzyme and BODIPY FL casein as the fluorogenic substrate. We demonstrated that the reaction rate of trypsin-BODIPY FL was significantly enhanced by increasing the local concentrations of both trypsin and BODIPY FL casein in the preconcentration chip. The reaction time required to turn over substrates at 1 ng/mL was only approximately 10 min compared to approximately 1 h without preconcentration, which demonstrates a significantly higher reaction rate through the increase of the concentrations of both the enzyme and substrate. Furthermore, trypsin activity can be measured down to a concentration level of 10 pg/mL, which is a approximately 100 fold enhancement in sensitivity compared to the result without the preconcentration step. This micro/nanofluidic preconcentrator chip could be used as a generic micro reaction platform to study any enzyme-substrate systems, or other biochemical reaction systems in low concentration ranges.