gebhart/patrepeval huggingface dataset:
Automated Announcements of Approaching Emergency Vehicles (2006)

Street intersections that are equipped with traffic lights would also be equipped with means for generating audible announcements of approaching emergency vehicles, according to a proposal. The means to generate the announcements would be implemented in the intersection- based subsystems of emergency traffic-light-preemption systems like those described in the two immediately preceding articles and in Systems Would Preempt Traffic Lights for Emergency Vehicles (NPO-30573), NASA Tech Briefs, Vol. 28, No. 10 (October 2004), page 36. Preempting traffic lights is not, by itself, sufficient to warn pedestrians at affected intersections that emergency vehicles are approaching. Automated visual displays that warn of approaching emergency vehicles can be helpful as a supplement to preemption of traffic lights, but experience teaches that for a variety of reasons, pedestrians often do not see such displays. Moreover, in noisy and crowded urban settings, the lights and sirens on emergency vehicles are often not noticed until a few seconds before the vehicles arrive. According to the proposal, the traffic-light preemption subsystem at each intersection would generate an audible announcement for example, emergency vehicle approaching, please clear intersection whenever a preemption was triggered. The subsystem would estimate the time of arrival of an approaching emergency vehicle by use of vehicle identity, position, and time data from one or more sources that could include units connected to traffic loops and/or transponders connected to diagnostic and navigation systems in participating emergency vehicles. The intersection-based subsystem would then start the announcement far enough in advance to enable pedestrians to leave the roadway before any emergency vehicles arrive.
Post-transcriptional Modification of RNAs by Artificial Box H/ACA and Box C/D RNPs (2011)

RNA-guided RNA 2'-O-methylation and pseudouridylation are naturally occurring processes, in which guide RNAs specifically direct modifications to rRNAs or spliceosomal snRNAs in the nucleus of eukaryotic cells. Modifications can profoundly alter the properties of an RNA, thus influencing the contributions of the RNA to the cellular process in which it participates. Recently, it has been shown that, by expressing artificial guide RNAs (derived from naturally occurring guide RNAs), modifications can also be specifically introduced into other RNAs, thus offering an opportunity to study RNAs in vivo. Here, we present strategies for constructing guide RNAs and manipulating RNA modifications in the nucleus.
The Quest for Tissue Stem Cells in the Pancreas and Other Organs, and their Application in Beta-Cell Replacement (2010)

Adult stem cell research has drawn a lot of attention by many researchers, due to its medical hope of cell replacement or regenerative therapy for diabetes patients. Despite the many research efforts to date, there is no consensus on the existence of stem cells in adult pancreas. Genetic lineage tracing experiments have put into serious doubt whether β-cell neogenesis from stem/progenitor cells takes place postnatally. Different in vitro experiments have suggested centroacinar, ductal, acinar, stellate, or yet unidentified clonigenic cells as candidate β-cell progenitors. As in the rest of the adult stem cell field, sound and promising observations have been made. However, these observations still need to be replicated. As an alternative to committed stem/progenitor cells in the pancreas, transdifferentiation or lineage reprogramming of exocrine acinar and endocrine α-cells may be used to generate new β-cells. At present, it is unclear which approach is most medically promising. This article highlights the progress being made in knowledge about tissue stem cells, their existence and availability for therapy in diabetes. Particular attention is given to the assessment of methods to verify the existence of tissue stem cells.
Design and operation of a scanning electrochemical microscope for imaging with continuous line probes (2019)

This article describes a home-built scanning electrochemical microscope capable of achieving high areal imaging rates through the use of continuous line probes (CLPs) and compressed sensing (CS) image reconstruction. The CLP is a nonlocal probe consisting of a band electrode, where the achievable spatial resolution is set by the thickness of the band and the achievable imaging rate is largely determined by its width. A combination of linear and rotational motors allows for CLP scanning at different angles over areas up to 25 cm<sup>2</sup> to generate the raw signal necessary to reconstruct the desired electrochemical image using CS signal analysis algorithms. Herein, we provide detailed descriptions of CLP fabrication, microscope design, and the procedures used to carry out scanning electrochemical microscopy imaging with CLPs. In order to illustrate the basic operating procedures for the microscope, line scans and images measured in the substrate generation-probe-collection mode for flat samples containing platinum disk electrodes are presented. These exemplary measurements illustrate methods for calibrating the positioning system, positioning and cleaning the CLP, and verifying proper positioning/probe sensitivity along its length.
Measuring the mass, density, and size of particles and cells using a suspended microchannel resonator (2007)

We demonstrate the measurement of mass, density, and size of cells and nanoparticles using suspended microchannel resonators. The masses of individual particles are quantified as transient frequency shifts, while the particles transit a microfluidic channel embedded in the resonating cantilever. Mass histograms resulting from these data reveal the distribution of a population of heterogeneously sized particles. Particle density is inferred from measurements made in different carrier fluids since the frequency shift for a particle is proportional to the mass difference relative to the displaced solution. We have characterized the density of polystyrene particles, Escherichia coli, and human red blood cells with a resolution down to 10−4g∕cm3.
A comparison of retinal prosthesis electrode array substrate materials (2009)

Simulations of artificial vision suggest that 1000 electrodes may be required to restore vision to individuals with diseases of the outer retina. In order to achieve such an implant, new technology is needed, since the state-of-the-art implantable neural stimulator has at most 22 contacts with neural tissue. A critical component of this system is the multi-channel, stimulating electrode array. This array must meet very challenging, competing requirements for manufacturing, integration, surgical handling, and biocompatibility. Our lab has evaluated 3 polymers as retinal prosthesis substrates: polyimide, parylene, and silicone.
High-Energy Sub-Optical-Cycle Parametric Waveform Synthesizer (2014)

We present FROG characterization of all three spectral channels of a multi-amplification-stage sub-optical-cycle parametric waveform synthesizer covering more than two octaves in bandwidth. The compressed 0.4-mJ energy can be scaled to the multi-mJ level.
Automatic generation and selection of multiple pronunciations for dynamic vocabularies (2002)

We present a scheme for the acoustic modeling of speech recognition applications requiring dynamic vocabularies. It applies especially to the acoustic modeling of out-of-vocabulary words which need to be added to a recognition lexicon based on the observation of a few (say one or two) speech utterances of these words. Standard approaches to this problem derive a single pronunciation from each speech utterance by combining acoustic and phone transition scores. In our scheme, multiple pronunciations are generated from each speech utterance of a word to enroll by varying the relative weights assigned to the acoustic and phone transition models. In our experiments, the use of these multiple baseforms dramatically outperforms the standard approach with a relative decrease of the word error rate ranging from 20% to 40% on all our test sets.
Formation of Ultrafine‐Grained Microstructure of Ti–6Al–4V Alloy by Hot Deformation of α′ Martensite Starting Microstructure (2011)

Abstract We have presented a formation of ultrafine‐grained microstructure (d α ≈ 0.2 µm) of industrial Ti–6Al–4V alloy produced by the hot compression of a sample with the acicular α′ martensite starting microstructure. The hot‐deformation behavior was different from the case of the conventional (α + β) starting microstructure, that is, the phase transformation of α′/(α + β) during hot working enhanced the microstructural conversion, especially under the conditions of a low temperature and a high‐strain rate.
Complement inhibition decreases the procoagulant response and confers organ protection in a baboon model of Escherichia coli sepsis (2010)

Severe sepsis leads to massive activation of coagulation and complement cascades that could contribute to multiple organ failure and death. To investigate the role of the complement and its crosstalk with the hemostatic system in the pathophysiology and therapeutics of sepsis, we have used a potent inhibitor (compstatin) administered early or late after Escherichia coli challenge in a baboon model of sepsis-induced multiple organ failure. Compstatin infusion inhibited sepsis-induced blood and tissue biomarkers of complement activation, reduced leucopenia and thrombocytopenia, and lowered the accumulation of macrophages and platelets in organs. Compstatin decreased the coagulopathic response by down-regulating tissue factor and PAI-1, diminished global blood coagulation markers (fibrinogen, fibrin-degradation products, APTT), and preserved the endothelial anticoagulant properties. Compstatin treatment also improved cardiac function and the biochemical markers of kidney and liver damage. Histologic analysis of vital organs collected from animals euthanized after 24 hours showed decreased microvascular thrombosis, improved vascular barrier function, and less leukocyte infiltration and cell death, all consistent with attenuated organ injury. We conclude that complement-coagulation interplay contributes to the progression of severe sepsis and blocking the harmful effects of complement activation products, especially during the organ failure stage of severe sepsis is a potentially important therapeutic strategy.
First identification of porcine parvovirus 6 in North America by viral metagenomic sequencing of serum from pigs infected with porcine reproductive and respiratory syndrome virus (2015)

These results are the first to report the presence of PPV6 in North America and demonstrate that the virus is found in multiple geographic areas in the United States and in Mexico. The overall prevalence of PPV6 in PRRSv viremic animals is relatively low. Further, all of the PPV6 genomes found in North America are most closely related to a PPV6 strain first identified in 2014 in healthy pigs from the Tianjin province of China.
Crystallization and preliminary X-ray diffraction analysis of the ternary human GM-CSF receptor complex (2008)

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a haemopoietic growth factor that acts though a ternary receptor signalling complex containing specific alpha (GMRalpha) and common beta (betac) receptor subunits. Human GM-CSF is encoded by the gene csf2, while the genes for GMRalpha and betac are csf2ra and csf2rb, respectively. Crystals of the ternary ectodomain complex comprising GM-CSF and the soluble extracellular regions of both the GMRalpha subunit and either betac or its glutamine-substitution mutant N346Q were obtained using the hanging-drop vapour-diffusion method. The best diffracting crystals of the ternary complex were obtained using the N346Q mutation of the betac subunit. These crystals grew using polyethylene glycol 3350 with a high concentration of proline, belonged to space group P6(3)22 and diffracted to 3.3 A resolution.
Semiconductor photonic integrated circuits (1991)

Semiconductor photonic integrated circuits (PICs) refer to that subset of optoelectronic integrated circuits (OEICs) which focus primarily on the monolithic integration of optically interconnected guided-wave optoelectronic devices. The principal motivation for PIC research is the expected cost reduction and packaging robustness associated with replacing individually aligned, single-mode optical connections between discrete optoelectronic devices with lithographically produced integrated waveguides. This field has recently seen significant advances resulting from improved III-V epitaxial crystal growth and related processing techniques. A discussion is presented of the design and fabrication issues, illustrated by a number of recently demonstrated InP-based PICs.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Neural Stem Cells for Spinal Cord Injury (2016)

Neural stem cells (NSCs) are potentially attractive cell sources for reconstruction of injured spinal cord circuits. Recent studies demonstrate that NSCs can survive grafting into sites of severe spinal cord injury (SCI) and extend very large numbers of axons over substantial distances, forming synapses with host neurons below sites of injury. Reciprocally, host axons regenerate into the stem cell grafts and form synapses. New synaptic relays are thereby formed across the lesion site, improving functional outcomes even after severe SCI. Additional studies are in progress to establish long-term safety and to scale up grafting methods to the larger primate system. Accordingly, this work is on a translational path.
Quantitative microbial ecology through stable isotope probing (2015)

Bacteria grow and transform elements at different rates, yet quantifying this variation in the environment is difficult. Determining isotope enrichment with fine taxonomic resolution after exposure to isotope tracers could help, but there are few suitable techniques. We propose a modification to Stable Isotope Probing (SIP) that enables determining the isotopic composition of DNA from individual bacterial taxa after exposure to isotope tracers. In our modification, after isopycnic centrifugation, DNA is collected in multiple density fractions, and each fraction is sequenced separately. Taxon specific density curves are produced for labeled and non-labeled treatments, from which the shift in density for each individual taxon in response to isotope labeling is calculated. Expressing each taxon’s density shift relative to that taxon’s density measured without isotope enrichment accounts for the influence of nucleic acid composition on density and isolates the influence of isotope tracer assimilation. The shift in density translates quantitatively to isotopic enrichment. Because this revision to SIP allows quantitative measurements of isotope enrichment, we propose to call it quantitative Stable Isotope Probing (qSIP). We demonstrate qSIP using soil incubations, in which soil bacteria exhibited strong taxonomic variation in 18 O and 13 C composition after exposure to 18 O-H 2 O or 13 C-glucose. Addition of glucose increased assimilation of 18 O into DNA from 18 O-H 2 O. However, the increase in 18 O assimilation was greater than expected based on utilization of glucose-derived carbon alone, because glucose addition indirectly stimulated bacteria to utilize other substrates for growth. This example illustrates the benefit of a quantitative approach to stable isotope probing.
<title>Microfluidic approaches to immunoassays</title> (1999)

An immunoassay format is presented that takes advantage of the microfluidic properties of the H-FilterTM for measuring sample analyte concentration. The method relies on the diffusion of analyte particles into a region containing beads coated with specific antibody. Competitive binding of labeled analyte and sample analyte with a limited number of binding sites allows measurement of the concentration of sample analyte based on the fraction of labeled analyte bound. The fraction of labeled analyte bound can be determined with a microcytometer by measuring the bead fluorescence intensity on the microcytometer portion of an integrated microfluidic chip. It is not necessary to separate the beads from the mixture because the bead intensity can be determined above the background of unbound labeled antigens. Other advantages include the ability to eliminate large interfering particles from samples, continuous sample monitoring, and the ability to concentrate the beads. Microfluidic immunoassay formats also consume smaller volumes of costly reagents and sample.© (1999) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Disulfide bond assignment and identification of regions required for functional activity of oncostatin M (1991)

Oncostatin M is a polypeptide cytokine having unique structure and diverse biological activities, including the ability to inhibit growth of certain cultured tumor cells. Here we have determined the disulfide bonding pattern of recombinant oncostatin M and have used site-directed mutagenesis to identify regions of this molecule necessary for receptor binding and growth inhibitory activities. Two intramolecular disulfide bonds, C6-C127 and C49-C167, were identified in recombinant oncostatin M. Analysis of mutations at each of the five cysteines in oncostatin M indicated that mutants C49S and C167S were inactive (less than 1/10 wild type activity) in growth inhibitory assays and radioreceptor assays. Carboxyl-terminal deletion mutations terminating at S185 and beyond were active, but further shortening abolished activity in both assays. Two deletion mutants proximal to C49 (delta 22-36 and delta 44-47) and insertion mutant GAG77 also were inactive. One deletion mutant, delta 87-90, had significantly (approximately 3-fold) increased activities in both growth inhibitory assays and radioreceptor assays. A potential amphiphilic domain was identified beginning at C167 and extending toward the carboxyl terminus. Two mutants having altered hydrophobic residues within this domain (F176G and F184G) were inactive, suggesting that these residues are required for proper conformation of the receptor binding site. Taken together, these results indicate that biological activity of oncostatin M requires discontinuous regions of the molecule, including residues near the essential disulfide bond, C49-C167, and within a putative amphiphilic helix at the carboxyl terminus. Oncostatin M thus belongs to a growing family of cytokines whose interactions with their respective receptors are mediated in part by known or predicted carboxyl-terminal amphiphilic helices.
Temporally feathered intensity modulated radiation therapy: a planning technique to reduce normal tissue toxicity (2017)

Abstract Purpose Intensity modulated radiation therapy (IMRT) has allowed optimization of three-dimensional spatial radiation dose distributions permitting target coverage while reducing normal tissue toxicity. However, radiation-induced normal tissue toxicity is a major contributor to patients’ quality of life and often a dose-limiting factor in the definitive treatment of cancer with radiation therapy. We propose the next logical step in the evolution of IMRT using canonical radiobiological principles, optimizing the temporal dimension through which radiation therapy is delivered to further reduce radiation-induced toxicity by increased time for normal tissue recovery. We term this novel treatment planning strategy “temporally feathered radiotherapy” (TFRT). Methods TFRT plans were generated as a composite of five simulated treatment plans each with altered constraints on particular hypothetical organs at risk (OARs) to be delivered sequentially. For each of these TFRT plans, OARs chosen for feathering receive higher doses while the remaining OARs receive lower doses than the standard fractional dose delivered in a conventional fractionated IMRT plan. Each TFRT plan is delivered a specific weekday, which in effect leads to a higher dose once weekly followed by four lower fractional doses to each temporally feathered OAR. We compared normal tissue toxicity between TFRT and conventional fractionated IMRT plans by using a dynamical mathematical model to describe radiation-induced tissue damage and repair over time. Results Model-based simulations of TFRT demonstrated potential for reduced normal tissue toxicity compared to conventionally planned IMRT. The sequencing of high and low fractional doses delivered to OARs by TFRT plans suggested increased normal tissue recovery, and hence less overall radiation-induced toxicity, despite higher total doses delivered to OARs compared to conventional fractionated IMRT plans. The magnitude of toxicity reduction by TFRT planning was found to depend on the corresponding standard fractional dose of IMRT and organ-specific recovery rate of sublethal radiation-induced damage. Conclusions TFRT is a novel technique for treatment planning and optimization of therapeutic radiotherapy that considers the non-linear aspects of tissue repair to optimize toxicity profiles. Model-based simulations of TFRT to carefully conceptualized clinical cases have demonstrated potential for normal tissue toxicity reduction in a previously described dynamical model of normal tissue complication probability (NTCP).
Line-by-Line Addressing of RMS Responding Matrix Displays With Wavelets (2007)

A line-by-line addressing technique that is based on wavelets is proposed to obtain gray shades in root-mean-square responding matrix displays. A large number of gray shades can be displayed with a smaller number of voltages (simple drivers) as compared with amplitude modulation and a smaller number of time intervals as compared with the frame modulation. The technique is demonstrated by displaying 128 gray shades in a liquid crystal display.
On Improving Battery State of Charge Estimation Using Bulk Force Measurements (2015)

Lithium-ion (Li-ion) batteries undergo physical deformation as their state-of-charge (SOC) changes. The physical deformation causes changes in the pressure (equivalently, force) applied at the end-plates of a constrained battery pack or module. This paper proposes the fusion of bulk force and battery voltage measurements to estimate the SOC in Li-ion battery packs. In this paper, using discrete Linear Quadratic Estimators (dLQEs), the advantage of using force measurements in addition to voltage measurement to improve SOC estimates is quantitatively studied through simulations. It is observed that including force measurements can decrease the mean and standard deviation of SOC estimation error by 50% in some SOC intervals.
Application of a Pulsed Detonation Engine for Electric Power Generation (2007)

A compact sized PDE with an i.d. of 0.75 in. and length of 1 m was paired with an automotive turbocharger. The turbine-compressor shaft was used to drive a small ac generator by means of speed reduction wheels. The PDE was tested with propane-oxygen mixture at 15 Hz for a period of about 20 s. The turbine spun up to a speed of 127,000 rpm, whilst the generator produced electric power at 27 W and the compressor pumped air at a rate of 0.055 kg/s. The exhaust of the turbine was measured to be 800 C, which implies that the exhaust has enough enthalpy to drive a few more turbine stages. The radial turbine results in more losses as it diverts the flow by 90 and its housing also creates hot spots. Axial turbines are better suited for application with PDEs and also enable better speed reduction gearing mechanisms to be applied, allowing larger and heavier generators to be used with turbines. The turbocharger did not exhibit any signs of damage after several minutes of testing. In subsequent tests with the PDE, detonations were observed for H2-O2 mixtures, but H2-Air mixtures failed to detonate.