gebhart/patrepeval huggingface dataset:
Seismic Retrofit of Precast Concrete Panel Connections with Carbon Fiber Reinforced Polymer Composites (2003)

A technique for the seismic retrofit and rehabilitation of existing welded steel plate edge connections between precast concrete shear wall panels using carbon fiber reinforced polymer (FRP) composite sheets is presented. Nine lateral load tests were performed on shear wall assemblies comprising three 4 x 12 ft x 8 in. (1.22 x 3.66 m x 203 mm) hollow-core precast concrete wall panels. Each wall assembly was loaded in the inplane direction by quasi-static simulated seismic loads. The test variables were the covered area of FRP composite sheets, the number and orientation of FRP composite layers, and wall panel surface preparation. Concrete surface preparation using high-pressure water jet and subsequent application of a bonding agent resulted in the most effective bond between concrete and FRP composite. The tests demonstrated that FRP composite connections could transfer the applied load across the joint effectively and achieve a higher capacity compared to welded steel plate edge connectors.
Figure S2 from TTK Inhibitors as a Targeted Therapy for <i>CTNNB1</i> (<b>β</b>-catenin) Mutant Cancers (2023)

<p>Relationship TTK inhibitor sensitivity and cancer gene mutant status</p>
Assembly of a Bacteriophage‐Based Template for the Organization of Materials into Nanoporous Networks (2014)

M13 bacteriophages are assembled via a covalent layer-by-layer process to form a highly nanoporous network capable of organizing nanoparticles and acting as a scaffold for templating metal-oxides. The morphological and optical properties of the film itself are presented as well as its ability to organize and disperse metal nanoparticles.
Drug Delivery: Exocytosis of Mesoporous Silica Nanoparticles from Mammalian Cells: From Asymmetric Cell‐to‐Cell Transfer to Protein Harvesting (Small 11/2011) (2011)

The cover picture shows magnetically doped mesoporous silica nanoparticles (MSNs) being first internalized by a cell (top) and adsorbing intracellular molecules (green). The particles are then exocytosed from the cell. Afterwards the ejected particles are either retrieved with a magnet to harvest the sequestered proteins, or taken up by a neighboring cell, which, if cancerous, could be less prone to exocytose the MSNs. These results suggest that complex targeting groups might not be necessary in the use of nanoparticles to treat cancer, and they may open a new avenue for the use of nanoparticles as tools to harvest proteins and biomolecules from living cells. For more information, please read the Communication “Exocytosis of Mesoporous Silica Nanoparticles from Mammalian Cells: From Asymmetric Cell-to-Cell Transfer to Protein Harvesting” by I. I. Slowing, B. G. Trewyn, and co-workers, beginning on page 1526.
Abstract 13707: Sodium Channel Blockers Prevent Triggered Activity but Not Abnormal Ca2+ Release in a Knock-in Mouse Model With Ryanodine Receptor Mutation R4496C (2010)

Catecholaminergic polymorphic ventricular tachycardia (CPVT) is an inherited disease characterized by adrenergically mediated polymorphic VT leading to syncope and sudden cardiac death. The present...
Quantum imaging of purely phase objects (2003)

Get PDF Email Share Share with Facebook Tweet This Post on reddit Share with LinkedIn Add to CiteULike Add to Mendeley Add to BibSonomy Get Citation Copy Citation Text A. V. Sergienko, A. F. Abouraddy, P. R. Stone, B. E. A. Saleh, and M. C. Teich, "Quantum Imaging of Purely Phase Objects," in Frontiers in Optics, OSA Technical Digest (CD) (Optica Publishing Group, 2003), paper ThB2. Export Citation BibTex Endnote (RIS) HTML Plain Text Citation alert Save article
Method for nuclear magnetic resonance imaging (1988)

Antinuclear antibodies (ANA) staining nuclear dot structures predominantly occur in primary biliary cirrhosis (PBC) patients and recognize the Sp100 and promyelocytic leukemia protein (PML). From retrospective analysis of sera from a clinically well-defined Canadian series of 170 PBC patients included into a 24-month therapeutic trial of ursodeoxycholic acid (UDCA), we report the prevalence of these ANA and their dynamics in the course of the disease. Using an enzyme-linked immunosorbent assay (ELISA), anti-Sp100 autoantibodies were shown in 35 (21%) patients. Thirty-three patients (19%) had autoantibodies against PML as determined by indirect immunostaining of cells overexpressing PML. Altogether, anti-nuclear dot autoantibodies were present in 25% of the 170 PBC patients. Their occurrence correlated with an unfavorable disease course, because these patients progressed significantly more frequently from early stages (I/II) to late stages (III/IV) within the 24-month observation period (P < .05). During the course of the disease, the autoantibody levels against the Sp100 full-length protein remained nearly constant in all 35 positive patients. However, 9 patients showed remarkable changes in Sp100 epitope recognition as revealed by ELISA and immunoblotting. When the occurrence of these changes and the treatment of the patients were compared retrospectively, it became evident that 8 of the 9 patients had received UDCA (42% of all Sp100-positive patients treated with UDCA). These findings indicate subtle changes of the Sp100 epitope recognition pattern during the natural course of the disease and its induction or acceleration by UDCA treatment. This implies that UDCA can modulate immunoglobulin (Ig) expression not only quantitatively, but also qualitatively.
A flexible package and interconnects for microfluidic systems (1999)

A slide-together compression package and microfluidic interconnects for microfabricated devices requiring fluidic and electrical connections is presented. The package assembles without tools, is reusable, and requires no epoxy, wirebonds, or solder, making chip replacement fast and easy. The microfluidic interconnects use standard HPLC PEEK tubing, with the tip machined to accept either an o-ring or custom molded ring which serves the dual function of forming the seal and providing mechanical retention strength. One design uses a screw to compress the o-ring, while others are simply plugged into a cartridge retained in the package. The connectors are helium leak-tight, can withstand hundreds of psi, are easy to connect and disconnect, are low dead volume, have a small footprint, and are adaptable to a broad range of microfabricated devices.
Evaluation of Multiple Models to Distinguish Closely Related Forms of Disease Using DNA Microarray Data: an Application to Multiple Myeloma (2004)

Logistic regression: standard software, available, for example in SAS. Decision trees and boosted trees: C5.0 from www.rulequest.com. SVM: SVM-light is publicly available from svmlight.joachims.org. Naïve Bayes and ensemble of voters are publicly available from www.biostat.wisc.edu/~mwaddell/eov.html. Nearest Shrunken Centroids is publicly available from http://www-stat.stanford.edu/~tibs/PAM.
Holographic memory using MEMS mirror beam steering technology (2003)

We describe the development of a high speed, high data rate holographic memory system using a photorefractive crystal as the storage media at JPL.
Quasi-Aromatic Heterocyclics As Laser Dyes (1989)

Superior laser dyes were recently discovered in the quasi-aromatic families of syn-dioxabimanes and pyrromethene-BF2 complexes. The representative new dye A-bis-(carboethoxy) methylene-syn-(methylene, methyl) bimane lased at 507 nm with 50% greater efficiency than was obtained with Coumarin 30. A similar determination showed 4,4-difluoro-1,3,5,7,8- pentamethy1-4-bora-3a,4a-diaza-s-indacene to lase at 546 nm with about 300% greater efficiency than was obtained from Coumarin 545. These quasi-aromatic systems are readily available and offer desirable properties of stability (photo, thermal, and chemical), solubility, and diminished triplet-triplet absorption in the lasing spectral region. Selected spectroscopic and lasing properties of these new laser dyes are presented.
ACPC: Efficient revocation of pseudonym certificates using activation codes (2018)

Vehicular communication (V2X) technologies allow vehicles to exchange information about the road conditions and their own status, and thereby enhance transportation safety and efficiency. For broader deployment, however, such technologies are expected to address security and privacy concerns, preventing abuse by users and by the system's entities. In particular, the system is expected to enable the revocation of malicious vehicles, e.g., in case they send invalid information to their peers or to the roadside infrastructure; it should also prevent the system from being misused for tracking honest vehicles. Both features are enabled by Vehicular Public Key Infrastructure (VPKI) solutions such as Security Credential Management Systems (SCMS), one of the leading candidates for protecting V2X communication in the United States. Unfortunately, though, SCMS's original revocation mechanism can lead to large Certification Revocation Lists (CRLs), which in turn impacts the bandwidth usage and processing overhead of the system. In this article, we propose a novel design called Activation Codes for Pseudonym Certificates (ACPC), which can be integrated into SCMS to address this issue. Our proposal is based on activation codes, short bit-strings without which certificates previously issued to a vehicle cannot be used by the latter, which are periodically distributed to non-revoked vehicles using an efficient broadcast mechanism. As a result, the identifiers of the corresponding certificates do no need to remain on the CRL for a long time, reducing the CRLs' size and streamlining their distribution and verification of any vehicle's revocation status. Besides describing ACPC in detail, we also compare it to similar-purpose solutions such as Issue First Activate Later (IFAL) and Binary Hash Tree based Certificate Access Management (BCAM). This analysis shows that our proposal not only improves privacy (e.g., in terms of resilience against colluding system authorities), but also leads to processing and bandwidth overheads that are orders of magnitude smaller than those observed in the state of the art.
High-Q HF microelectromechanical filters (2000)

IC-compatible microelectromechanical intermediate frequency filters using integrated resonators with Q's in the thousands to achieve filter Q's in the hundreds have been demonstrated using a polysilicon surface micromachining technology. These filters are composed of two clamped-clamped beam micromechanical resonators coupled by a soft flexural-mode mechanical spring. The center frequency of a given filter is determined by the resonance frequency of the constituent resonators, while the bandwidth is determined by the coupling spring dimensions and its location between the resonators. Quarter-wavelength coupling is required on this microscale to alleviate mass loading effects caused by similar resonator and coupler dimensions. Despite constraints arising from quarter-wavelength design, a range of percent bandwidths is still attainable by taking advantage of low-velocity spring attachment locations. A complete design procedure is presented in which electromechanical analogies are used to model the mechanical device via equivalent electrical circuits. Filter center frequencies around 8 MHz with Q's from 40 to 450 (i.e., percent bandwidths from 0.23 to 2.5%), associated insertion losses less than 2 dB, and spurious-free dynamic ranges around 78 dB are demonstrated using low-velocity designs with input and output termination resistances of the order of 12 k/spl Omega/.
Fluorescence polarisation of green fluorescent protein (GFP). A strategy for improved wavelength discrimination for GFP determinations (1999)

The fluorescence of green fluorescent protein (GFP), present both within whole yeast cells and in protein extracts from yeast cells, has been observed to be significantly polarised. Fluorescence polarisation is proposed as a useful technique to allow some discrimination between GFP fluorescence and that of other interfering species in cell or media matrices, which have fluorescence bands that overlap those of GFP, which should lead to improved resolution and limits of detection. The method has been tested by discriminating between the fluorescence of GFP in cell extracts and added fluorescein, both of which fluoresce brightly at the same wavelength. The flow-through instrumentation incorporating an argon-ion laser developed for this work is also described.
Manufacturability Oriented Model Correction and Build Direction Optimization for Additive Manufacturing (2019)

Abstract We introduce a method to analyze and modify a shape to make it manufacturable for a given additive manufacturing (AM) process. Different AM technologies, process parameters, or materials introduce geometric constraints on what is manufacturable or not. Given an input 3D model and minimum printable feature size dictated by the manufacturing process characteristics and parameters, our algorithm generates a corrected geometry that is printable with the intended AM process. A key issue in model correction for manufacturability is the identification of critical features that are affected by the printing process. To address this challenge, we propose a topology aware approach to construct the allowable space for a print head to traverse during the 3D printing process. Combined with our build orientation optimization algorithm, the amount of modifications performed on the shape is kept at minimum while providing an accurate approximation of the as-manufactured part. We demonstrate our method on a variety of 3D models and validate it by 3D printing the results.
A method for rapid, continuous monitoring of solute uptake and binding (1982)

A method has been developed for dynamically monitoring the free concentration of diffusible, tritiated solutes. The technique utilizes particles of a solid scintillator microencapsulated in gel beads that are permeable to diffusible label. Since tritium beta radioactivity has an effective range in water of only a few micrometers, only label that is free to diffuse through the gel can excite the scintillator, while sequestered label is effectively excluded. Thus, the scintillation light output monitors the freely diffusible concentration of label exclusively. A simple, preliminary encapsulation technique is described and tested, and the theory behind the method is discussed with regard to the time resolution attainable for a given label concentration and type of encapsulation. The feasibility of the method is demonstrated by measuring the uptake of [3H]tetraphenylphosphonium by Escherichia coli membrane vesicles in response to the generation of a membrane potential (interior negative).
Real-Signal Mother Wavelet and Its Application on Detection of Abnormal Signal (Designing Average Complex Real-Signal Mother Wavelet and Its Application) (2007)

In this study, in order to improve effectiveness of the abnormal signal detection, the technique of constituting an Average Real signal Mother Wavelet (A-RMW) from two or more real signals has been proposed and its effectiveness has been confirmed. The main results obtained can be summarized as follows : (1) For constructing A-RMW by two or more signals, the Symmetric Complex Real signal Mother Wavelet (SCRMW) first was constructed respectively and then the average SCRMW was obtained by adding them. Finally the average SCRMW obtained above was normalized and the A-RMW was obtained. (2) The proposed constructing method of the SCRMW is different from the traditional Complex Real signal Mother Wavelet that the phase information of all the frequency components has been cut off and symmetric property can be obtained. (3) The proposal method is applied to the rattle noise source detection of a car, and its effectiveness is examined.
Random high-Q cavities in disordered photonic crystal waveguides (2007)

We present direct observations of electromagnetic fields localized in disordered photonic crystal waveguides and report the modal volumes and quality factors of the confined modes. Geometrical perturbations distributed uniformly throughout the crystal lattice were introduced by changing orientations of the polygonal lattice elements. Cavities in the disordered waveguides were excited by resonant coupling through a chain of random open resonators. Localized optical resonances with sub-(λ∕n)3 modal volumes and quality factors of up to ∼150000 were observed.
Deterministic Quantum Emitter Formation in Hexagonal Boron Nitride via Controlled Edge Creation (2019)

Quantum emitters (QEs) in 2D hexagonal boron nitride (hBN) are extremely bright and are stable at high temperature and under harsh chemical conditions. Because they reside within an atomically thin 2D material, these QEs have a unique potential to couple strongly to hybrid optoelectromechanical and quantum devices. However, this potential for coupling has been underexplored because of challenges in nanofabrication and patterning of hBN QEs. Motivated by recent studies showing that QEs in hBN tend to form at edges, we use a focused ion beam (FIB) to mill an array of patterned holes into hBN. Using optical confocal microscopy, we find arrays of bright, localized photoluminescence that match the geometry of the patterned holes. Furthermore, second-order photon correlation measurements on these bright spots reveal that they contain single and multiple QEs. By optimizing the FIB parameters, we create patterned single QEs with a yield of 31%, a value close to Poissonian limit. Using atomic force microscopy to study the morphology near emission sites, we find that single QE yield is highest with smoothly milled holes on unwrinkled hBN. This technique dramatically broadens the utility and convenience of hBN QEs and achieves a vital step toward the facile integration of the QEs into large-scale photonic, plasmonic, nanomechanical, or optoelectronic devices.
Antibodies binding to discrete segments of CϵmX of mIgE on B lymphocytes (P6004) (2013)

Abstract CϵmX is a discrete domain of 52 a.a. residues, located between the CH4 domain and the C-terminal membrane anchor peptide of the ϵ heavy chain of membrane-bound IgE (mIgE) on human B cells. Among the monoclonal antibodies (mAbs) prepared for their ability to bind to CϵmX fragments or CϵmX-containing recombinant proteins in ELISA, those that can bind to native mIgE on B cells can cause ADCC, apoptosis, and other cytolytic mechanisms on the B cells and are potential useful for controlling IgE production for treating allergic diseases. The CϵmX-specific mAbs prepared previously appear to recognize discrete and overlapping peptide segments of CϵmX. A linear peptide epitope of an antibody is defined by a contiguous peptide segment of minimal stretch that confers maximum binding by the antibody. In some instances, while the peptide epitopes recognized by two mAbs may be adjacent to each other and overlap, the two mAbs do not bind to the adjacent epitopes by a substantial degree. This can be illustrated by the pair of humanized mAbs, h4B12 and h47H4. The mAb h4B12 binds to peptide P1-15 (GLAGGSAQSQRAPDR) and h47H4 binds to peptide P7-18 (AQSQRAPDRVLC). Although the two epitopes share a segment AQSQRAPDR, the mAb h4B12 does not bind to P7-18, and h47H4 does not bind to P1-15. A related presentation from our group (Hsing-Mao Chu et al.) will also reveal that the shared peptide segment AQSQRAPDR has two markedly different conformations when it is associated with h4B12 or with h47H4.
Proteome Analysis and Functional Expression Identify Mortalin as an Antiapoptotic Gene Induced by Elevation of [Na <sup>+</sup> ] <sub>i</sub> /[K <sup>+</sup> ] <sub>i</sub> Ratio in Cultured Vascular Smooth Muscle Cells (2002)

Apoptosis of vascular smooth muscle cells (VSMCs) plays an important role in remodeling of vessel walls, one of the major determinants of long-term blood pressure elevation and an independent risk factor for cardiovascular morbidity and mortality. Recently, we have found that apoptosis in cultured VSMCs can be inhibited by inversion of the intracellular [Na+]/[K+] ratio after the sustained blockage of the Na+,K+-ATPase by ouabain. To understand the mechanism of ouabain action, we analyzed subsets of hydrophilic and hydrophobic VSMC proteins from control and ouabain-treated cells by 2-dimensional electrophoresis. Ouabain treatment led to overexpression of numerous soluble and hydrophobic cellular proteins. Among proteins that showed the highest level of ouabain-induced expression, we identified mortalin (also known as GRP75 or PBP-74), a member of the heat shock protein 70 (HSP70) superfamily and a marker for cellular mortal and immortal phenotypes. Northern and Western blotting and immunocytochemistry all have confirmed that treatment of VSMCs with ouabain results in potent induction of mortalin expression. Transient transfection of cells with mortalin cDNA led to at least a 6-hour delay in the development of apoptosis after serum deprivation. The expression of tumor suppressor gene, p53, in mortalin-transfected cells was delayed to the same extent, and the expressed protein showed abnormal perinuclear distribution, suggesting that p53 is retained and inactivated by mortalin. Our studies therefore define a new [Na+]i/[K+]i-responsive signaling pathway that may play an important role in the regulation of programmed cell death in VSMCs.
Induction of T-cell development by Delta-like 4-expressing fibroblasts (2013)

The thymus provides a unique environment for the induction of T-cell lineage commitment and differentiation, which is predicted by specific Notch ligand-receptor interactions on epithelial cells and lymphoid progenitors, respectively. Accordingly, a bone marrow-derived stromal cell line (OP9) ectopically expressing the Notch ligand Delta-like 1 (Dll1) or Dll4 (OP9-DL1 and OP9-DL4, respectively) gains the ability to recapitulate thymus-like function, supporting T-cell differentiation of both mouse and human progenitors. In this study, we extend these findings by demonstrating that, unlike the NIH3T3 cell line, mouse primary fibroblasts made to express Dll4 (mFibro-DL4) acquire the capacity to promote and support T-cell development from hematopoietic stem cells (HSCs) into TCR + , CD4 + and CD8 + T-lineage cells. However, mFibro-DL4 cells showed a lower efficiency of T-cell generation than OP9-DL4 cells did. Nevertheless, progenitor T-cells (CD117 + CD44 + CD25 + ) generated in HSC/mFibro-DL4 co-cultures, when intravenously transferred into immunodeficient (Rag2 -/-c -/-) mice, home to the thymus, undergo differentiation, and give rise to mature T-cells that go on to populate the periphery. Surprisingly, primary human fibroblast cells expressing Dll4 showed very low efficiency in supporting human T-lineage differentiation, which could not be improved by blocking myelopoiesis. Nevertheless, mFibro-DL4 cells could support human T-cell lineage differentiation. Our results provide a functional framework for the induction of T-cell development using easily accessible fibroblasts made to express Dll4. These cells, which are amenable for in vitro applications, can be further utilized in the design of individualized systems for T-cell production, with implications for the treatment of immunodeficiencies.
ChemInform Abstract: PREPARATION OF 2‐HYDRAZINOBENZOTHIAZOLES BY EXCHANGE AMINATION (1975)

Abstract Die angegebene Umaminierung stellt eine allgemeine Methode zur Darstellung substituierter 2‐Hydrazinobenzothiazole (II) aus den entsprechenden 2‐Arninöbenzothiazolen (I).
A 3-D Active Contour Method for Automated Segmentation of the Left Ventricle From Magnetic Resonance Images (2016)

A true 3-D reconstruction technique without the need for training datasets or any user-driven segmentation has been developed. In this method, a novel combination of internal and external energy terms for active contour was utilized that exploits histogram matching for improving the segmentation performance. This method takes advantage of full volumetric imaging, does not rely on prior statistical knowledge, and employs a convex-hull interpolation to include the papillary muscles.