gebhart/patrepeval huggingface dataset:
Nanogel-based pneumococcal surface protein A nasal vaccine induces microRNA-associated Th17 cell responses with neutralizing antibodies against Streptococcus pneumoniae in macaques (2015)

We previously established a nanosized nasal vaccine delivery system by using a cationic cholesteryl group-bearing pullulan nanogel (cCHP nanogel), which is a universal protein-based antigen-delivery vehicle for adjuvant-free nasal vaccination. In the present study, we examined the central nervous system safety and efficacy of nasal vaccination with our developed cCHP nanogel containing pneumococcal surface protein A (PspA-nanogel) against pneumococcal infection in nonhuman primates. When [(18)F]-labeled PspA-nanogel was nasally administered to a rhesus macaque (Macaca mulatta), longer-term retention of PspA was noted in the nasal cavity when compared with administration of PspA alone. Of importance, no deposition of [(18)F]-PspA was seen in the olfactory bulbs or brain. Nasal PspA-nanogel vaccination effectively induced PspA-specific serum IgG with protective activity and mucosal secretory IgA (SIgA) Ab responses in cynomolgus macaques (Macaca fascicularis). Nasal PspA-nanogel-induced immune responses were mediated through T-helper (Th) 2 and Th17 cytokine responses concomitantly with marked increases in the levels of miR-181a and miR-326 in the serum and respiratory tract tissues, respectively, of the macaques. These results demonstrate that nasal PspA-nanogel vaccination is a safe and effective strategy for the development of a nasal vaccine for the prevention of pneumonia in humans.
Polypeptide specificity of the antibody response after primary and recurrent infection with bovine herpesvirus 1 (1986)

The polypeptide specificities and defense mechanisms of the humoral immune response to bovine herpesvirus 1 were analyzed. Sequential serum samples taken from cows which were experimentally infected with bovine herpesvirus 1 were tested for their reactivity with individual bovine herpesvirus 1 polypeptides by immunoprecipitation, immunoblotting, and enzyme-linked immunosorbent assay. All bovine immune sera reacted with each of the three major bovine herpesvirus 1 glycoproteins, GVP 6/11a/16, GVP 3/9, and GVP 11b, during primary as well as recurrent infection. Among these glycoproteins, GVP 6/11a/16 induced the earliest and most consistent immune response. The levels of antibody to GVP 3/9 and GVP 11b varied among the animals, and they were slightly lower than the level of antibody to GVP 6/11a/16. Antibodies to several nonglycosylated polypeptides and two additional glycoproteins were also detected with the immunoblot assay. However, these antibodies were usually apparent only during recurrent infection, whereas they were undetectable or low during primary infection. The antibodies in the sera from all animals mediated virus neutralization and destruction of virus-infected cells by two immune mechanisms, e.g., antibody- and complement-mediated lysis and antibody-dependent cell-mediated cytotoxicity.
Microscopic Void Detection for Predicting Remaining Life in Electric Cable Insulation (2003)

A reliable method of testing for remaining life in electric cable insulation has continued to elude the nuclear industry as it seeks to extend the life and license of its nuclear stations. Until recently, a trendable, measurable electrical property has not been found, and unexpected cable failures continue to be reported. Most reliable approaches to date rely on monitoring mechanical properties, which are assumed to degrade faster than the insulation’s electrical properties. This paper introduces a promising technique based on void characterization, which is dependent on an electrical property related to dielectric strength. A relationship between insulation void characteristics (size and density) and the onset of partial discharge is known to exist. A similar relationship can be shown between void characteristics and unacceptable leakage currents (another typical cable failure criterion). For low-voltage cables, it is believed void content can be correlated to mechanical property degradation.This paper will report on an approach for using void information, research results showing the existence of trendable void characteristics in commonly used electric insulation materials, and techniques for detecting the voids (both laboratory- and field-based techniques). Acoustical microscopy was found to be potentially more suitable than conventional ultrasound for nondestructive in situ detection and monitoring of void characteristics in jacketed multiconductor insulation while ignoring the jacket. Also, optical and scanning electron microscope techniques will play an essential role in establishing the database necessary for continued development and implementation of this promising technique.
A Case Study on Power Outage Impacts from Future Hurricane Sandy Scenarios (2017)

Abstract Hurricane Sandy (2012, referred to as Current Sandy) was among the most devastating storms to impact Connecticut’s overhead electric distribution network, resulting in over 15 000 outage locations that affected more than 500 000 customers. In this paper, the severity of tree-caused outages in Connecticut is estimated under future-climate Hurricane Sandy simulations, each exhibiting strengthened winds and heavier rain accumulation over the study area from large-scale thermodynamic changes in the atmosphere and track changes in the year ~2100 (referred to as Future Sandy). Three machine-learning models used five weather simulations and the ensemble mean of Current and Future Sandy, along with land-use and overhead utility infrastructure data, to predict the severity and spatial distribution of outages across the Eversource Energy service territory in Connecticut. To assess the influence of increased precipitation from Future Sandy, two approaches were compared: an outage model fit with a full set of variables accounting for both wind and precipitation, and a reduced set with only wind. Future Sandy displayed an outage increase of 42%–64% when using the ensemble of WRF simulations fit with three different outage prediction models. This study is a proof of concept for the assessment of increased outage risk resulting from potential changes in tropical cyclone intensity associated with late-century thermodynamic changes driven by the IPCC AR4 A2 emissions scenario.
A General Approach to the Basiliolide/Transtaganolide Natural Products: Total Syntheses of Basiliolide B, <i>epi</i>‐8‐Basiliolide B, Transtaganolide C, and Transtaganolide D (2011)

Blitzschnell: Die Totalsynthese von Transtaganolid- und Basiliolid-Naturstoffen gelingt in drei Stufen ausgehend von achiralen monocyclischen Estern (siehe Schema). Entscheidende Schritte in der Synthese sind eine Ireland-Claisen/Diels-Alder-Kaskade und eine neuartige Sequenz aus Methoxyacetylidkupplung und Cyclisierung.
Registration of ‘UA103’ Cotton Cultivar (2013)

‘UA103’ (Reg. No. CV‐131, PI 664928) is a conventional open‐canopy (okra‐leaf) cultivar of cotton ( Gossypium hirsutum L.), that was released by the Arkansas Agricultural Experiment Station in July 2011. The cultivar was released as part of an ongoing effort to develop improved genotypes having enhanced yield, yield components, earliness, host‐plant resistance, and fiber properties. UA103 expressed earlier maturation (as indicated by the percentage of open bolls rated after first defoliation) than ‘DP 393’, which is considered a short‐season cultivar. When evaluated over years and locations, lint yields of UA103 were equal to those of DP 393, but lint yields of UA103 were lower than DP 393 at Keiser and Marianna. The lower yields at Keiser may be related to the very early maturity of UA103. Lint yields of UA103 were the result of greater lint per seed but fewer seed per area. Lint yields of UA103 in tests outside of Arkansas were highly variable, which may be related to its maturity. UA103 displays high resistance to bacterial blight [caused by Xanthomonas axonopodis pv. malvacearum (Smith) Dye] but is more sensitive than DP 393 to tarnished plant bug [ Lygus lineolaris (Palisot de Beauvois)]. UA103 exhibited longer fibers and lower micronaire than DP 393. These fiber qualities are positively correlated with better spinning performance. UA103 displays a specific combination of high yield ability in certain environments, very early maturity, high fiber quality, and host‐plant resistance traits.
MmpL3 as a Target for the Treatment of Drug-Resistant Nontuberculous Mycobacterial Infections (2018)

Nontuberculous mycobacterial (NTM) pulmonary infections are emerging as a global health problem and pose a threat to susceptible individuals with structural or functional lung conditions such as cystic fibrosis, chronic obstructive pulmonary disease and bronchiectasis. <i>Mycobacterium avium</i> complex (MAC) and <i>Mycobacterium abscessus</i> complex (MABSC) species account for 70-95% of the pulmonary NTM infections worldwide. Treatment options for these pathogens are limited, involve lengthy multidrug regimens of 12-18 months with parenteral and oral drugs, and their outcome is often suboptimal. Development of new drugs and improved regimens to treat NTM infections are thus greatly needed. In the last 2 years, the screening of compound libraries against <i>M. abscessus</i> in culture has led to the discovery of a number of different chemotypes that target MmpL3, an essential inner membrane transporter involved in the export of the building blocks of the outer membrane of all mycobacteria known as the mycolic acids. This perspective reflects on the therapeutic potential of MmpL3 in <i>Mycobacterium tuberculosis</i> and NTM and the possible reasons underlying the outstanding promiscuity of this target. It further analyzes the physiological and structural factors that may account for the apparent looser structure-activity relationship of some of these compound series against <i>M. tuberculosis</i> compared to NTM.
Reference Governor for Load Control in a Multicylinder Recompression HCCI Engine (2013)

This paper presents a model-based control strategy designed to regulate combustion phasing during load transitions in a recompression homogeneous charge compression ignition (HCCI) engine. A low-order discrete-time control-oriented model for recompression HCCI combustion is developed that represents the strong thermal and composition coupling between engine cycles. A baseline two-input single-output controller is designed to regulate combustion phasing, using the amount of negative valve overlap and the fuel injection timing as actuators. This controller is augmented by a reference or fuel governor, which modifies transient fuel mass commands during large load transitions, when future actuator constraint violations are predicted. This approach is shown in experiments to improve combustion phasing and load responses, preventing engine misfires in some cases. The fuel governor enables larger load transitions than were possible with the baseline controller alone. The governor acts only when future actuator constraint violations are predicted. The complexity and computational overhead of the governor are reduced by developing a linearized fuel governor. Satisfactory performance is demonstrated experimentally for a range of engine speeds.
Face Recognition in the Dark (2005)

Previous research has established thermal infrared imagery of faces as a valid biometric and has shown high recognition performance in a wide range of scenarios. However, all these results have been obtained using eye locations that were either manually marked, or automatically detected in a coregistered visible image, making the realistic use of thermal infrared imagery alone impossible. In this paper we present the results of an eye detector on thermal infrared imagery and we analyze its impact on recognition performance. Our experiments show that although eyes cannot be detected as reliably in thermal images as in visible ones, some face recognition algorithms can still achieve adequate performance.
Effect of carbon dioxide on methane oxidative coupling kinetics (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEffect of carbon dioxide on methane oxidative coupling kineticsKevin J. Smith and Jan GaluszkaCite this: Ind. Eng. Chem. Res. 1994, 33, 1, 14–20Publication Date (Print):January 1, 1994Publication History Published online1 May 2002Published inissue 1 January 1994https://pubs.acs.org/doi/10.1021/ie00025a003https://doi.org/10.1021/ie00025a003research-articleACS PublicationsRequest reuse permissionsArticle Views170Altmetric-Citations13LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Effect of fish oil, arginine, and doxorubicin chemotherapy on remission and survival time for dogs with lymphoma (2000)

BACKGROUND Polyunsaturated n-3 fatty acids have been shown to inhibit the growth and metastasis of tumors. This double-blind, randomized study was designed to evaluate the hypothesis that polyunsaturated n-3 fatty acids can improve metabolic parameters, decrease chemical indices of inflammation, enhance quality of life, and extend disease free interval and survival time for dogs treated for lymphoblastic lymphoma with doxorubicin chemotherapy. METHODS Thirty-two dogs with lymphoma were randomized to receive one of two diets supplemented with menhaden fish oil and arginine (experimental diet) or an otherwise identical diet supplemented with soybean oil (control diet). Diets were fed before and after remission was attained with up to five dosages of doxorubicin. Parameters examined included blood concentrations of glucose, lactic acid, and insulin in response to glucose and diet tolerance tests; alpha-1 acid glycoprotein; tumor necrosis factor; interleukin-6; body weight; amino acid profiles; resting energy expenditure; disease free interval (DFI); survival time (ST); and clinical performance scores. RESULTS Dogs fed the experimental diet had significantly (P < 0.05) higher mean serum levels of the n-3 fatty acids docosahexaenoic acid (C22:6) and eicosapentaenoic acid (C20:5) compared with controls. Higher serum levels of C22:6 and C20:5 were associated with lesser (P < 0.05) plasma lactic acid responses to intravenous glucose and diet tolerance testing. Increasing C22:6 levels were significantly (P < 0.05) associated with longer DFI and ST for dogs with Stage III lymphoma fed the experimental diet. CONCLUSIONS Fatty acids of the n-3 series normalize elevated blood lactic acid in a dose-dependent manner, resulting in an increase in DFI and ST for dogs with lymphoma. Cancer 2000;88:1916–28. © 2000 American Cancer Society.
COPEPTIN, A STABLE PEPTIDE OF THE ARGININE VASOPRESSIN PRECURSOR, IS ELEVATED IN HEMORRHAGIC AND SEPTIC SHOCK (2007)

Arginine vasopressin (AVP) levels are increased in hemorrhagic and septic shock. Measurement of AVP levels has limitations due to its short half-life and cumbersome detection method. Copeptin is a more stable peptide derived from the same precursor molecule. We evaluated the plasma copeptin concentration in two independent studies: first, in an experimental baboon model of hemorrhagic shock, and second, in a prospective observational study of 101 consecutive critically ill patients at a university hospital. Copeptin was measured with a newly developed sandwich immunoassay using two polyclonal antibodies to the C-terminal region (amino acid sequence 132-164) of pre-pro-AVP. Copeptin concentrations in hemorrhagic shock increased markedly from median (range) of 7.5 [2.7-13) to 269 pM (241-456 pM). After reperfusion, copeptin levels dropped within hours to a plateau of 27 pM (15-78 pM). In the critically ill patient cohort, copeptin values increased significantly with the severity of the disease and were in patients without sepsis [27.6 pM [2.3-297 pM]), in sepsis [50.0 pM [8.5-268 pM]), in severe sepsis [73.6 pM [15.3-317 pM]), and in septic shock [171.5 pM (35.1-504 pM] compared with 4.1 pM (1.0-13.8 pM) in healthy controls (P for all vs. controls <0.001). On admission, circulating copeptin levels were higher in nonsurvivors (171.5 pM, 46.5-504.0 pM) as compared with survivors (86.8 pM, 8.5-386.0 pM; P = 0.01). Copeptin levels correlated with basal cortisol levels (r = 0.42; P < 0.001) and osmolality (r = 0.42; P < 0.001). In a logistic regression model including other covariates besides copeptin (e.g., determinants of fluid status) on survival, serum copeptin levels were the only independent significant predictor of outcome (P = 0.03). Copeptin concentrations are elevated in hemorrhagic and septic shock. Copeptin was higher on admission in nonsurvivors as compared with survivors, suggesting copeptin as a prognostic marker in sepsis. The availability of a reliable assay for the measurement of AVP release can also prove useful for the assessment of fluid and osmosis status in various diseases.
Specific Surface Area by Colorimetry and Image Analysis (2021)

Abstract Specific surface area is more informative than grain size for fine-grained sediments where Ss &gt; 1 m2/g. In fact, specific surface area plays a central role in engineering properties and processes in fine-grained soils, including pore size and bioactivity; fabric, plasticity, and rheology; hydraulic and electrical conductivity; compressibility and residual friction angle; and all forms of coupled processes. This research advanced a dye adsorption method using digital image colorimetry implemented with smartphone technology. In particular, this research adopted a water-based approach to reach internal surfaces in platy phyllosilicates, selected short dye molecules to reduce the range of potential molecular contact area, and developed a physics-based adsorption model to analyze the complete data set to minimize the uncertainty in specific surface area determinations. The study involved fine-grained soils with distinct mineralogy and specific surface area (from 1 to 600 m2/g) and various cationic dyes and a protein to explore the effect of molecular size, shape, and pH. Crystal violet emerged as a reliable dye for soil characterization. Time-dependent measurements confirmed second-order kinetics and highlight the importance of adsorption time.
Characterization Of The HLA Class II Ligandome In Acute Myeloid Leukemia (AML) Reveals Novel Candidates For Peptide-Based Immunotherapy (2013)

Abstract CD4+ T cells are crucial for the induction and maintenance of cytotoxic T cell responses, but can also mediate direct tumor rejection. The therapeutic efficacy of peptide-based cancer vaccines may thus be improved by including HLA class II epitopes to stimulate T helper cells. In contrast to HLA class I ligands, only a small number of class II ligands of TAA has been described so far. We recently reported on the overexpression of HLA class II in AML cells as compared to autologous monocytes and granulocytes as well as on the first HLA class I leukemia associated antigens identified directly on the cell surface of primary AML cells (Stickel et. al. abstract in Blood 2012). In this study we characterized the HLA class II ligandome in AML to identify additional ligands for a peptide-based immunotherapy approach. HLA class II ligands from primary AML cells as well as bone marrow and peripheral blood mononuclear cell (BMNCs/PBMCs) of healthy donors were analyzed using the approach of direct isolation and identification of naturally presented HLA peptides by affinity chromatography and mass spectrometry (LC-MS/MS). LC-MS/MS peptide analysis provided qualitative and semi-quantitative information regarding the composition of the respective ligandomes. Comparative analysis of malignant and benign samples served to identify ligandome-derived tumor associated antigens (LiTAAs) and to select peptide vaccine candidates. Most abundantly detected peptides were functionally characterized with regard to their ability to induce a specific CD4+ T-cell response in healthy donors and in tumor patients using ELISpot. Samples from 10 AML patients (5 FLT3-ITD mutated) and 18 healthy donors were analyzed. We identified more than 2,100 AML-derived HLA class II ligands representing >1,000 different source proteins, of which 315 were exclusively represented in AML, but not in healthy PBMC/BMNC. Data mining for broadly represented LiTAAs pinpointed 26 HLA class II ligands from 8 source proteins that were presented exclusively on more than 40% of all analyzed AML samples as most promising targets. Amongst them were already described TAAs (e.g., RAB5A) as well as several so far understated proteins (e.g. calsyntenin 1, glycophorin A, mannose-binding lectin 2). Subset analysis revealed 58 LiTAAs presented exclusively on FLT3-ITD mutated AML cells. Additional screening for HLA class II ligands from described leukemia associated antigens showed positive results for NPM1 (1 peptide sequence) and MPO (13 peptide sequences). Peptides from calsyntenin 1 and RAB5A were able to elicit CD4+-T-cell response in 25% of tested AML patients (n=16). Thus, our study identified, for the first time, HLA class II tumor associated antigens directly obtained from the HLA ligandomes of AML patients and thereby represents a further step to our goal of developing a multipeptide vaccine for immunotherapy of AML. Disclosures: No relevant conflicts of interest to declare.
Semantic Interior Mapology: A Toolbox For Indoor Scene Description From Architectural Floor Plans (2019)

We introduce the Semantic Interior Mapology (SIM) toolbox for the conversion of a floor plan and its room contents (such as furnitures) to a vectorized form. The toolbox is composed of the Map Conversion toolkit and the Map Population toolkit. The Map Conversion toolkit allows one to quickly trace the layout of a floor plan, and to generate a GeoJSON file that can be rendered in 3D using web applications such as Mapbox. The Map Population toolkit takes the 3D scan of a room in the building (acquired from an RGB-D camera), and, through a semi-automatic process, populates individual objects of interest with a correct dimension and position in the GeoJSON representation of the building. SIM is easy to use and produces accurate results even in the case of complex building layouts.