gebhart/patrepeval huggingface dataset:
Design and Synthesis of a FlAsH-Type Mg<sup>2+</sup> Fluorescent Probe for Specific Protein Labeling (2014)

Although the magnesium ion (Mg(2+)) is one of the most abundant divalent cations in cells and is known to play critical roles in many physiological processes, its mobilization and underlying mechanisms are still unknown. Here, we describe a novel fluorescent Mg(2+) probe, "KMG-104-AsH", composed of a highly selective fluorescent Mg(2+) probe, "KMG-104", and a fluorescence-recoverable probe, "FlAsH", bound specifically to a tetracysteine peptide tag (TCtag), which can be genetically incorporated into any protein. This probe was developed for molecular imaging of local changes in intracellular Mg(2+) concentration. KMG-104-AsH was synthesized, and its optical properties were investigated in solution. The fluorescence intensity of KMG-104-AsH (at λ(em/max) = 540 nm) increases by more than 10-fold by binding to both the TCtag peptide and Mg(2+), and the probe is highly selective for Mg(2+) (K(d/Mg) = 1.7 mM, K(d/Ca) ≫ 100 mM). Application of the probe for imaging of Mg(2+) in HeLa cells showed that this FlAsH-type Mg(2+) sensing probe is membrane-permeable and binds specifically to tagged proteins, such as TCtag-actin and mKeima-TCtag targeted to the cytoplasm and the mitochondrial intermembrane space. KMG-104-AsH bound to TCtag responded to an increase in intracellular Mg(2+) concentration caused by the release of Mg(2+) from mitochondria induced by FCCP, a protonophore that eliminates the inner membrane potential of mitochondria. This probe is expected to be a strong tool for elucidating the dynamics and mechanisms of intracellular localization of Mg(2+).
Effects of glucagon-like peptide 1 on appetite and body weight: focus on the CNS (2013)

The delivery of nutrients to the gastrointestinal tract after food ingestion activates the secretion of several gut-derived mediators, including the incretin hormone glucagon-like peptide 1 (GLP-1). GLP-1 receptor agonists (GLP-1RA), such as exenatide and liraglutide, are currently employed successfully in the treatment of patients with type 2 diabetes mellitus. GLP-1RA improve glycaemic control and stimulate satiety, leading to reductions in food intake and body weight. Besides gastric distension and peripheral vagal nerve activation, GLP-1RA induce satiety by influencing brain regions involved in the regulation of feeding, and several routes of action have been proposed. This review summarises the evidence for a physiological role of GLP-1 in the central regulation of feeding behaviour and the different routes of action involved. Also, we provide an overview of presently available data on pharmacological stimulation of GLP-1 pathways leading to alterations in CNS activity, reductions in food intake and weight loss.
Faecal microbiota transplantation protects against radiation‐induced toxicity (2017)

Severe radiation exposure may cause acute radiation syndrome, a possibly fatal condition requiring effective therapy. Gut microbiota can be manipulated to fight against many diseases. We explored whether intestinal microbe transplantation could alleviate radiation-induced toxicity. High-throughput sequencing showed that gastrointestinal bacterial community composition differed between male and female mice and was associated with susceptibility to radiation toxicity. Faecal microbiota transplantation (FMT) increased the survival rate of irradiated animals, elevated peripheral white blood cell counts and improved gastrointestinal tract function and intestinal epithelial integrity in irradiated male and female mice. FMT preserved the intestinal bacterial composition and retained mRNA and long non-coding RNA expression profiles of host small intestines in a sex-specific fashion. Despite promoting angiogenesis, sex-matched FMT did not accelerate the proliferation of cancer cells <i>in vivo</i> FMT might serve as a therapeutic to mitigate radiation-induced toxicity and improve the prognosis of tumour patients after radiotherapy.
An ultra-low power capacitor-less LDO for always-on domain in NB-IoT applications (2018)

This paper presents an ultra-low power 55 nm CMOS capacitor-less Low-Dropout (LDO) voltage regulator for power management of an Always-On domain module for implementation of NB-IoT SoC applications. Compared with traditional IoT SoC, the power consumption specification of NB-IoT SoC is lower. One effective way to achieve low power application is to reduce the power of the Always-On domain module in the SoC, where the LDO plays a critical role. According to the tape-out, results are validated with a 0.89V output voltage from 1.8V to 3.3V supply voltage, delivering a load current of 100uA. The peak-peak variance of output voltage is less than 100mV from 1.8V to 3.3V supply voltage. The quiescent current is lower than 61.86nA, including a 2-Transistor reference voltage, which can implement NB-IoT SoC application, with yield as high as 87.88%.
A solution for a mobile computing device along with supporting infrastructure for the needs of illiterate users in rural areas (2012)

This paper proposes an integrated solution using a mobile computing device suitable for use by illiterate people in mainly rural areas. Modifications in supported applications including telephony, messaging and browser to enable them to be used text free are described. Images, animations and speech recognition are used instead of text, as well as WebRTC for real time communication. Infrastructure needed to support the use of such devices in rural areas is also considered, as well as a revenue sharing model to enable such infrastructure. We show it is possible to manufacture a phone customized to such users within a reasonable price and using existing technology. Our approach is to use technology to empower illiterate people at a level which they can presently relate to, rather than focus on technology to promote literacy.
Numerical analysis of efficiency droop induced by piezoelectric polarization in InGaN/GaN light-emitting diodes (2010)

The effects of piezoelectric polarization on efficiency droop in InGaN/GaN light-emitting diodes (LEDs) have been investigated using numerical analysis. The simulation results showed that the severe band bending in InGaN quantum-well was improved as the piezoelectric polarization is reduced, resulting in the improved overlap of electron and hole wave functions. As a results, the internal quantum efficiency increases and efficiency droop significantly reduces. The reduction in piezoelectric polarization could be derived by applying a tensile stress to relax compressive stress in GaN epilayer, improving the efficiency droop of vertical-structure LEDs, agree well with simulation ones.
Severe Cutaneous Reactions among American Travelers Using Pyrimethamine-Sulfadoxine (Fansidar®) for Malaria Prophylaxis (1986)

In 1982, the fixed combination of pyrimethamine and sulfadoxine (Fansidar) became available in the United States, and was recommended for use in travelers at risk of acquiring chloroquine-resistant Plasmodium falciparum. Prior to that time, no reports of severe cutaneous reactions had appeared in the medical literature despite widespread use for more than 8 years in both Europe and malarious areas of the developing world. In the fall of 1984, the Centers for Disease Control received reports of 4 cases of toxic epidermal necrolysis (including 3 fatalities) among Americans who had used pyrimethamine-sulfadoxine (PYR/SDX) for the prevention of malaria. Subsequent investigation into severe cutaneous reactions associated with the use of this drug by American travelers detected 24 cases of erythema multiforme, Stevens-Johnson syndrome, or toxic epidermal necrolysis. Twenty-three of the 24 patients concurrently used chloroquine. Seven patients died. No risk factors in the development of these reactions other than the use of PYR/SDX could be identified. Among American travelers, we estimate that these reactions occur in 1 per 5,000-8,000 users, and that fatal reactions occur in 1 per 11,000-25,000 users. This higher than expected incidence necessitates that the use of PYR/SDX for the prevention of malaria be reconsidered. In the United States it is now recommended that the routine weekly use of the drug be reserved for those travelers at highest risk of acquiring chloroquine-resistant P. falciparum, when alternate prophylactic regimens are not deemed appropriate.
Cloud networked robotics (2012)

This article proposes a new field of research called Cloud Networked Robotics, which tackles the issues for supporting daily activity, especially for the elderly and the disabled, throughout various locations in a continuously and seamless manner by abstracting robotic devices and providing a means for utilizing them as a cloud of robots. With recent advances in robotic development environments and in integrated multi-robot systems, robots are acquiring richer functionalities and robotic systems are becoming much easier to develop. However, such stand-alone robotic services are not enough for continuously and seamlessly supporting daily activity. We examine the requirements in typical daily supporting services through example scenarios that target senior citizens and the disabled. Based on these requirements, we discuss the key research issues in cloud network robotics. As a case study, a field experiment in a shopping mall shows how our proposed prototype infrastructure of cloud networked robotics enables multi-location robotic services for life support.
Comparison of Flat and Hollow‐Fiber Mixed‐Matrix Composite Membranes for CO<sub>2</sub> Separation with Temperature (2017)

Abstract Zeolite A/poly (1‐trimethylsilyl‐1‐propyne) (zeoliteA/PTMSP) and [emim][Ac]/chitosan (IL/CS) are mixed‐matrix membrane (MMM) materials with enhanced CO 2 /N 2 permselectivity even at higher temperature. The scalability to asymmetric flat and hollow‐fiber geometry by a simple dip‐coating method was analyzed. The CO 2 /N 2 separation performance was evaluated at different temperatures. The resulting composite membranes exhibit a significantly enhanced CO 2 permeation flux because the MMM layer thickness is reduced by 97 % from flat to hollow‐fiber geometries in IL‐CS composite membranes, while the selectivity is maintained similar to the self‐standing membranes, thus proving that compatibility between the membrane component materials leads to a defect‐free composite membrane, regardless the geometry and temperature.
The relative biological effectiveness in V79 Chinese hamster cells of the neutron capture reactions in boron and nitrogen. (1984)

V79 Chinese hamster cells were irradiated in the presence of different amounts of boric acid with thermal neutrons at the Medical Research Reactor at Brookhaven National Laboratory. From the linear dose-survival curves observed, a D0 value of 66 rad for the 10B(n, alpha) 7Li neutron capture reaction was obtained. No dependence of this value on the concentration of boric acid was found. Comparing this value to the D0 value of 150 rad obtained with 250 kVp X rays between 10 and 0.01% survival, an extrapolated RBE value of 2.3 was calculated. By irradiation of the same line of cells with cold neutrons at the Institut Laue - Langevin , a D0 value for the 14N(n,p)14C reaction of 77 rad was obtained, with a corresponding RBE value of 1.9. Comparison is made with previously published RBE values for the 10B(n, alpha) 7Li reaction.
Selection of disease‐specific biomarkers by integrating inflammatory mediators with clinical informatics in AECOPD patients: a preliminary study (2011)

Systemic inflammation is a major factor influencing the outcome and quality of patient with chronic obstructive pulmonary disease (COPD) and acute exacerbations (AECOPD). Because of the inflammatory complexity, a great challenge is still confronted to optimize the identification and validation of disease-specific biomarkers. This study aimed at developing a new protocol of specific biomarker evaluation by integrating proteomic profiles of inflammatory mediators with clinical informatics in AECOPD patients, understand better their function and signal networks. Plasma samples were collected from healthy non-smokers or patients with stable COPD (sCOPD) or AECOPD on days 1 and 3 of the admission and discharging day (day 7-10). Forty chemokines were measured using a chemokine multiplex antibody array. Clinical informatics was achieved by a Digital Evaluation Score System (DESS) for assessing severity of patients. Chemokine data was compared among different groups and its correlation with DESS scores was performed by SPSS software. Of 40 chemokines, 30 showed significant difference between sCOPD patients and healthy controls, 16 between AECOPD patients and controls and 13 between AECOPD patients and both sCOPD and controls, including BTC, IL-9, IL-18Bpa, CCL22,CCL23, CCL25, CCL28, CTACK, LIGHT, MSPa, MCP-3, MCP-4 and OPN. Of them, some had significant correlation with DESS scores. There is a disease-specific profile of inflammatory mediators in COPD and AECOPD patients which may have a potential diagnostics together with clinical informatics of patients. Our preliminary study suggested that integration of proteomics with clinical informatics can be a new way to validate and optimize disease-special biomarkers.
A novel approach to induce cell cycle reentry in terminally differentiated muscle cells (2001)

During terminal differentiation, skeletal muscle cells permanently retract from the cell cycle. We and others have shown previously that this cell cycle withdrawal is an actively maintained state that can be reversed by transient expression of the SV40 large T antigen. In an attempt to avoid the hazards of gene transfer and the difficulties of regulating transgene expression, we have now used this cellular system as a model to test whether direct protein delivery could constitute a feasible alternative or complementing strategy to gene therapy-based approaches. Taking advantage of the recently described intercellular trafficking properties of the herpes simplex virus I VP22 protein, we have constructed a chimeric VP22-SV40 large T antigen fusion protein and shown that it can spread into terminally differentiated myotubes where it accumulates in the nucleus. This fusion protein retains the ability to override the cell cycle arrest as shown for SV40 large T antigen alone. Our results clearly show that the transduced fusion protein remains capable of inducing S-phase and mitosis in these otherwise terminally differentiated cells and opens now the way to exploit this novel strategy for tissue regeneration.
<sup>13</sup>C-NMR, MS and metabolic flux balancing in biotechnology research (1998)

The European Federation of Biotechnology defines biotechnology as ‘the integration of natural sciences and engineering sciences in order to achieve the application of organisms, cells, parts thereof and molecular analogues for products and services’. Biotechnology thus focuses on the industrial exploitation of biological systems and is based on their unique expertise in specific molecular recognition and catalysis. The enormous potential for drug synthesis, design of biomedical diagnostics, large-scale production of biochemicals including fuels, food production, degradation of resistant wastes and extraction of raw materials will very likely make biotechnology, along with electronics and material sciences, one of the key technologies of the 21st century. From the chemical engineer's point of view, the living system participating in a biotechnological process is the central unit that catalyses chemical reactions. It exhibits a complex dependence on the bioprocess parameters, and the engineer focuses on these parameters to achieve optimal control (Hamer, 1985; Bailey & Ollis, 1986). For the natural scientist, the living system itself is in the centre of interest, so that attempts to optimize a bioprocess aim at its appropriate redesign by genetic manipulations. The increase in penicillin production by strain improvement based on random mutagenesis, which was pursued from 1940 to the mid 1970s, represents an early contribution of life scientists to improve a bioprocess that is of utmost medical importance (Hardy & Oliver, 1985).
A meta‐analysis of immunogenetic Case–Control Association Studies in irritable bowel syndrome (2015)

Our meta-analysis could not confirm a major role of most investigated SNPs, but a moderate association between rs4263839 TNFSF15 and IBS, in particular IBS-C. The analysis emphasizes the importance of definition and phenotype homogeneity, adequate study size and representativeness of the patient and control collective.
Morpholino oligomers tested in vitro, in biofilm and in vivo against multidrug-resistant Klebsiella pneumoniae (2018)

PPMOs have the potential to be effective therapeutic agents against KPC-expressing, MDR K. pneumoniae.
Urea-inducible Egr-1 transcription in renal inner medullary collecting duct (mIMCD3) cells is mediated by extracellular signal-regulated kinase activation. (1996)

Urea (200-400 milliosmolar) activates transcription, translation of, and trans-activation by the immediate-early gene transcription factor Egr-1 in a renal epithelial cell-specific fashion. The effect at the transcriptional level has been attributed to multiple serum response elements and their adjacent Ets motifs located within the Egr-1 promoter. Elk-1, a principal ternary complex factor and Ets domain-containing protein, is a substrate of the extracellular signal-regulated kinase (ERK) mitogen-activated protein kinases. In the renal medullary mIMCD3 cell line, urea (200-400 milliosmolar) activated both ERK1 and ERK2 as determined by in-gel kinase assay and immune-complex kinase assay of epitope-tagged] ERK1 and ERK2. Importantly, urea did not affect abundance of either ERK. Urea-inducible Egr-1 transcription was a consequence of ERK activation because the ERK-specific inhibitor, PD98059, abrogated transcription from the murine Egr-1 promoter in a luciferase reported gene assay. In addition, activators of protein kinase A, including forskolin and 8-Br-cAMP, which are known to inhibit ERK-mediated events, also inhibited urea-inducible Egr-1 transcription. Furthermore, urea-inducible activation of the physiological ERK substrate and transcription factor, Elk-1, was demonstrated through transient cotransfection of a chimeric Elk-1/GAL4 expression plasmid and a GAL4-driven luciferase reporter plasmid. Taken together, these data indicate that, in mIMCD3 cells, urea activates ERKs and the ERK substrate, Elk-1, and that ERK inhibition abrogates urea-inducible Egr-1 transcription. These data are consistent with a model of urea-inducible renal medullary gene expression wherein sequential activation of ERKs and Elk-1 results in increased transcription of Egr-1 through serum response element/Ets motifs.
Pustular psoriasis in pregnancy, and prednisolone (1995)

A case of generalized pustular psoriasis in pregnancy treated effectively with systemic corticosteroids is reported. A live, healthy infant was born at 36 weeks gestation. A further severe episode of pustular psoriasis occurred after cessation of corticosteroids, but was treated successfully with methotrexate and isotretinoin. The treatment of generalized pustular psoriasis in pregnancy is discussed.
A series of vectors for fungal transformation (1997)

We report a new fungal selectable marker that confers resistance to chlorimuron ethyl, a sulfonylurea herbicide. This gene as well as genes that confer resistance to hygromycin and bialaphos have been engineered to be compact and to eliminate sites for most common restriction enzymes. These three selectable markers have been used to construct a series of vectors for fungal transformation.
Electrochemical Measurement of O<sub>2</sub> Permeation Rate through Polymer Electrolyte Membranes (2009)

Abstract not Available.
Growth of mesenchymal stromal cells in automated microwell cultures : influence of the engineering environment on cell growth kinetics and non-directed differentiation (2009)

Human Mesenchymal Stromal Cells (hMSC) have the potential to differentiate into lineages of mesoderm origin, such as osteogenic, chondrogenic, and adipogenic lineages, presenting a promising potential for autologous regenerative medicine applications. However, one of the major challenges associated with delivering hMSC to the clinic is the propagation of undifferentiated cells in vitro in order to reach the quantity required for therapeutic applications. This thesis investigates the effect of environmental parameters that impact cell growth characteristics of hMSC in microwell plates, for the design of an automated cell expansion process in a robotic platform. As a result of this work, the main parameters that can be used to control the growth rate and differentiation potential of hMSC at all stages of the cell expansion process have been identified. A mathematical model has also been developed to forward predict the cell growth characteristic of hMSC for an individual donor, allowing for a patient specific bioprocess design that will ensure enough cells can be supplied back to the patient in a timely manner while assuring the quality of the final product.\nProcess parameters for the cell expansion process of hMSC in automated microwells have been characterised. Optimum values for inoculation cell density and medium exchange strategies have been proven to reduce the overall time of the cell expansion process for hMSC in an undifferentiated state for their use in regenerative medicine therapies. These parameters were implemented in the cell expansion in an automated platform for the duration of one passage, proving the potential of such technology for the delivery of hMSC to the clinic.
Liquid-liquid equilibrium of aqueous mixtures of poly(propylene glycol) with sodium chloride (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTLiquid-liquid equilibrium of aqueous mixtures of poly(propylene glycol) with sodium chlorideEric L. Cheluget, Stephanie Gelinas, Juan H. Vera, and Martin E. WeberCite this: J. Chem. Eng. Data 1994, 39, 1, 127–130Publication Date (Print):January 1, 1994Publication History Published online1 May 2002Published inissue 1 January 1994https://pubs.acs.org/doi/10.1021/je00013a036https://doi.org/10.1021/je00013a036research-articleACS PublicationsRequest reuse permissionsArticle Views638Altmetric-Citations112LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts