gebhart/patrepeval huggingface dataset:
Improvement of Power Factor in Switched Reluctance Motor Drives Through Optimizing the Switching Angles (2004)

Presented in this article is a novel control strategy to improve the power factor of switched reluctance motor drives through optimizing the turn-on angle and the turn-off angle by using the optimization algorithms. Both the simulation and experimental results validate the proposed control strategy. Through the comparisons and the analyses of the reporting results, the two schemes to improve the power factor are developed. The proposed schemes are suitable for both small and large ratings of SRM drives.
At Grandma's Home. Interview by Charlotte Alderman. (2005)

Among African countries, Uganda has been help up as an example in tackling HIV/AIDS. Ruth Sims has pioneered care and treatment for many in need.
An Overview of MPEG Immersive Video (2021)

MPEG immersive video is an upcoming standard for data access and delivery of immersive content. A brief introduction of its encoding and decoding processes is presented along with a simplified playback implementation of it demonstrated on Intel GPUs.
I/O Virtualization (2011)

The term virtual is heavily overloaded, evoking everything from virtual machines running in the cloud to avatars running across virtual worlds. Even within the narrowfigureer context of computer I/O, virtualization has a long, diverse history, exemplified by logical devices that are deliberately separate from their physical instantiations.
Preparation of Highly Ordered Nitrogen‐Containing Mesoporous Carbon from a Gelatin Biomolecule and its Excellent Sensing of Acetic Acid (2012)

Abstract Novel nitrogen‐containing mesoporous carbon with well‐ordered pores (NMC‐G) and high basicity is synthesized using a low‐cost single‐molecule precursor, gelatin biomolecule, and SBA‐15 as a template via nanocasting method. The obtained materials are thoroughly characterized. It is found that the prepared materials have excellent textural properties such as high specific surface areas, huge pore volumes, and large pore diameters. The pore diameter of the materials can also be controlled with a simple adjustment of the pore diameter of the hard templates. The C/N ratio of the samples is calculated to be ≈6.01, which is slightly higher than that observed for mesoporous carbon nitride samples. X‐ray photoelectron spectroscopy (XPS) reveals the presence of sp 2 hybridized carbon in aromatic ring structure attached to amino groups. The materials could adsorb a huge quantity of CO 2 . The sensing capability of the materials with different pore diameters for different adsorbates including ethanol, acetic acid, aniline, toluene, and ammonia is also investigated. Among the materials with different pore diameters studied, the material with the highest basicity and the largest pore diameter (NMC‐G‐150) showed excellent sensing performance with a high selectivity of adsorption for acetic acid molecule.
Experimental Study of Thermal and Catalytic Pyrolysis of Plastic Waste Components (2018)

Thermal and catalytic pyrolysis of virgin low-density polyethylene (LDPE), high-density polyethylene (HDPE), polypropylene (PP) and mixtures of LDPE/PP were carried out in a 200 mL laboratory scale batch reactor at 460 °C in a nitrogen atmosphere. Thermogravimetric analysis (TGA) was carried out to study the thermal and catalytic degradation of the polymers at a heating rate of 10 °C/min. The amount of PP was varied in the LDPE/PP mixture to explore its effect on the reaction. In thermal degradation (TGA) of LDPE/PP blends, a lower decomposition temperature was observed for LDPE/PP mixtures compared to pure LDPE, indicating interaction between the two polymer types. In the presence of a catalyst (CAT-2), the degradation temperatures for the pure polymers were reduced. The TGA results were validated in a batch reactor using PP and LDPE, respectively. The result from thermal pyrolysis showed that the oil product contained significant amounts of hydrocarbons in the ranges of C7–C12 (gasoline range) and C13–C20 (diesel range). The catalyst enhanced cracking at lower temperatures and narrowed the hydrocarbon distribution in the oil towards the lower molecular weight range (C7–C12). The result suggests that the oil produced from catalytic pyrolysis of waste plastics has a potential as an alternative fuel.
A purinergic P2Y6 receptor agonist prodrug modulates airway inflammation, remodeling, and hyperreactivity in a mouse model of asthma (2018)

The P2Y6R prodrug GC021109 inhibited allergen-induced changes in pulmonary function, inflammatory responses, and airway and vascular smooth muscle mass. P2Y6R activation may be an effective therapeutic maintenance strategy in asthma.
Constellation Tending: An Application of On-Orbit Servicing and Active Debris Removal Technologies (2020)

This paper will discuss the application of on-orbit servicing to address the needs of establishing and maintaining large satellite constellations such as those currently being developed for global communications, and earth observation. These developments face many challenges, two of which are tightly intertwined: assuring reliable, global, persistent coverage, via large production lots for satellite constellations, and yet limiting the creation of orbital debris. While the regulatory environment surrounding orbital debris is still unclear, there is a strong incentive for all parties to establish adequate guidelines for protecting the Low Earth Orbit environment, and to explore achievable solutions for meeting these guidelines. In this paper, we will describe a candidate on-orbit servicing architecture, which can address orbital debris concerns in a robust and economical way, and also reduce constellation establishment costs through reduction in satellite complexity and improvements in launch and mission operations.
Sterile Protection against Malaria Is Independent of Immune Responses to the Circumsporozoite Protein (2007)

We conclude that other pre-erythrocytic parasite antigens, possibly hitherto uncharacterised, can be targeted to induce sterile immunity against malaria. From a broader perspective, our results raise the question as to whether immunodominant parasite antigens should be the favoured targets for vaccine development.
Probiotics in the Treatment and Prevention of Atopic Dermatitis (2010)

Research Articles| September 08 2010 Probiotics in the Treatment and Prevention of Atopic Dermatitis Subject Area: Endocrinology , Further Areas , Nutrition and Dietetics , Public Health R. Fölster-Holst R. Fölster-Holst Universitätsklinikum Schleswig-Holstein, Campus Kiel, Kiel, Germany Search for other works by this author on: This Site PubMed Google Scholar Ann Nutr Metab (2010) 57 (Suppl. 1): 16–19. https://doi.org/10.1159/000309054 Article history Published Online: September 08 2010 Content Tools Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Tools Icon Tools Get Permissions Cite Icon Cite Search Site Citation R. Fölster-Holst; Probiotics in the Treatment and Prevention of Atopic Dermatitis. Ann Nutr Metab 1 September 2010; 57 (Suppl. 1): 16–19. https://doi.org/10.1159/000309054 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsAnnals of Nutrition and Metabolism Search Advanced Search Article PDF first page preview Close Modal © 2010 S. Karger AG, Basel2010Copyright / Drug Dosage / DisclaimerCopyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. You do not currently have access to this content.
A wideband metamaterial-inspired compact antenna using embedded non-Foster matching (2011)

Passive electrically small antennas have a small radiation resistance and a narrow bandwidth. In this paper, a new type of active antenna is reported in which an active circuit generating a non-Foster impedance is embedded in a metamaterial-inspired small antenna with an inherent sizable radiation resistance. This circuit interacts with the reactive elements of the antenna. The end result is a compact, broadband antenna which is well matched and has the potential for a high efficiency. In this process, the need for an active (or passive) step-up transformer for the radiation resistance is eliminated. This work opens up the possibility of utilizing non-Foster components embedded in metamaterial-based antenna structures to obtain efficient antennas by manipulating the current and field distribution in and around the antenna.
Reconstruction of plasmid DNA with Red-mediated homologous recombination combined with restriction digestion (2005)

Objective:To precisely delete a 2 100 bp DNA sequence in pBR322-Red plasmid using Red mediated single-stranded oligonucleotides in vivo homologous recombination combined with restriction digestion strategy.Methods:A single-stranded oligonucleotides DNA target cassette was introduced into W3110(pBR322-Red) host strain by electroporation,a 2 100 bp DNA fragment from kil to N gene that contains a unique XhoI site in pBR322-Red plasmid was deleted by Red mediated in vivo homologous recombination. The recombinant plasmid DNA was screened from the plasmid DNA mixture with XhoⅠ restriction endonuclease digestion,and retransformed into W3110 competent cells. The positive clones were further (identified) by colony PCR assay.Results: One positive clone out of ten clones was successfully identified without any selectable marker. Conclusion:This new method of DNA sequence deletion mutation is simple and accurate. It could avoid new mutation in PCR process of traditional DNA manipulation method.
Engineering a protein-protein interface using a computationally designed library (2020)

Computational algorithms for protein design can sample large regions of sequence space, but suffer from undersampling of conformational space and energy function inaccuracies. Experimental screening of combinatorial protein libraries avoids the need for accurate energy functions, but has limited access to vast amounts of sequence space. Here, we test if these two traditionally alternative, but potentially complementary approaches can be combined to design a variant of the ubiquitin-ligase E6AP that will bind to a nonnatural partner, the NEDD8-conjugating enzyme Ubc12. Three E6AP libraries were constructed: (i) a naive library in which all 20 amino acids were allowed at every position on the target-binding surface of E6AP (13 positions), (ii) a semidirected library that varied the same residue positions as in the naive library but disallowed mutations computationally predicted to destabilize E6AP, and (iii) a directed library that used docking and sequence optimization simulations to identify mutations predicted to be favorable for binding Ubc12. Both of the directed libraries showed > 30-fold enrichment over the naive library after the first round of screening with a split-dihydrofolate reductase complementation assay and produced multiple tight binders (Kd < 100 nM) after four rounds of selection. Four rounds of selection with the naive library failed to produce any binders with Kd’s lower than 50 μM. These results indicate that protein design simulations can be used to create directed libraries that are enriched in tight binders and that in some cases it is sufficient to computationally screen for well-folded sequences without explicit binding calculations.
Exposure to siRNA-GalNAc Conjugates in Systems of the Standard Test Battery for Genotoxicity (2016)

Registration of pharmaceuticals requires an assessment of their genotoxic potential using in vitro and in vivo tests outlined in the International Conference on Harmonisation (ICH) guidance S2(R1). We have evaluated numerous siRNA-N-acetylgalactosamine (GalNAc) conjugates containing phosphorothioate linkages and various combinations of 2'-fluoro and 2'-O-methyl ribose modifications of multiple nucleotides in the ICH battery of assays, all of which have uniformly yielded negative results. To verify these negative genotoxicity results, in this study we confirm test article exposure using toolkit small interfering RNAs (siRNAs) representative of those in the clinic. In the Ames test, the highest uptake of the siRNA-GalNAc conjugates occurred at 1 h postdose in all bacterial strains independent of siRNA sequence or chemistry (up to ∼14,000 siRNA molecules per cell), followed by metabolic degradation of the parent siRNA at 6, 24, and 48 h postdose. siRNA-GalNAc conjugates were internalized by bacteria as assessed by protection from the addition of nucleases to the culture media following uptake and by the requirement of cell lysis for detection of the siRNA. In the in vitro chromosome aberration assay, uptake was observed in Chinese hamster ovary cells (up to ∼5,500 siRNA molecules per cell at 21 h postdose) and in CD3<sup>+</sup> human peripheral blood lymphocytes (up to ∼500 siRNA molecules per cell at 21 h postdose). In the in vivo micronucleus assay in rat bone marrow, exposure to parent siRNA was 100-350 μg of antisense strand per gram of protein at 24 and 48 h postlimit dose of 2 g/kg. Loss of terminal nucleotides was detected in bone marrow by mass spectrometry, indicating exposure to monomer metabolites as well. Negative genotoxicity results were also confirmed in an in vitro double-strand DNA break assay in HeLa and HepG2 cells where exposure was maximized using transfection reagents. Thus negative genotoxicity assay results for siRNA-GalNAc conjugates were valid and not the result of poor or no intracellular exposure.
Surface localization of Helicobacter pylori urease and a heat shock protein homolog requires bacterial autolysis (1996)

Helicobacter pylori is a gram-negative bacterium which causes chronic gastritis and is associated with peptic ulcer disease, gastric carcinoma, and gastric lymphoma. The bacterium is characterized by potent urease activity, thought to be located on the outer membrane, which is essential for survival at low pH. The purpose of the present study was to investigate mechanisms whereby urease and HspB, a GroEL homolog, become surface associated in vitro. Urease, HspB, and catalase were located almost exclusively within the cytoplasm in fresh log-phase cultures assessed by cryo- immunoelectron microscopy. In contrast, significant amounts of surface-associated antigen were observed in older or subcultured preparations concomitantly with the appearance of significant amounts of extracellular antigen, amorphous debris, and membrane fragments. By use of a variety of biochemical methods, a significant fraction of urease and HspB was associated with the outer membrane in subcultured preparations of H. pylori. Taken together, these results strongly suggest that H. pylori cells undergo spontaneous autolysis during culture and that urease and HspB become surface associated only concomitant with bacterial autolysis. By comparing enzyme sensitivity to flurofamide (a potent, poorly diffusible urease inhibitor) in whole cells with that in deliberately lysed cells, we show that both extracellular and intracellular urease molecules are active enzymatically. Autolysis of H. pylori is an important phenomenon to recognize since it likely exerts significant effects on the behavior of H. pylori. Furthermore, the surface properties of H. pylori must be unique in promoting adsorption of cytoplasmic proteins.
A Human Embryonic Kidney 293T Cell Line Mutated at the Golgi α-Mannosidase II Locus (2009)

Disruption of Golgi alpha-mannosidase II activity can result in type II congenital dyserythropoietic anemia and induce lupus-like autoimmunity in mice. Here, we isolated a mutant human embryonic kidney (HEK) 293T cell line called Lec36, which displays sensitivity to ricin that lies between the parental HEK 293T cells, in which the secreted and membrane-expressed proteins are dominated by complex-type glycosylation, and 293S Lec1 cells, which produce only oligomannose-type N-linked glycans. Stem cell marker 19A was transiently expressed in the HEK 293T Lec36 cells and in parental HEK 293T cells with and without the potent Golgi alpha-mannosidase II inhibitor, swainsonine. Negative ion nano-electrospray ionization mass spectra of the 19A N-linked glycans from HEK 293T Lec36 and swainsonine-treated HEK 293T cells were qualitatively indistinguishable and, as shown by collision-induced dissociation spectra, were dominated by hybrid-type glycosylation. Nucleotide sequencing revealed mutations in each allele of MAN2A1, the gene encoding Golgi alpha-mannosidase II: a point mutation that mapped to the active site was found in one allele, and an in-frame deletion of 12 nucleotides was found in the other allele. Expression of the wild type but not the mutant MAN2A1 alleles in Lec36 cells restored processing of the 19A reporter glycoprotein to complex-type glycosylation. The Lec36 cell line will be useful for expressing therapeutic glycoproteins with hybrid-type glycans and as a sensitive host for detecting mutations in human MAN2A1 causing type II congenital dyserythropoietic anemia.
Canonical variate analysis in identification, filtering, and adaptive control (1990)

The canonical variate analysis (CVA) approach for system identification, filtering, and adaptive control is developed. The past/future Markov property provides a starting point for defining a reduced-order prediction problem. The solution is a canonical variate analysis that is characterized by a generalized singular value decomposition. State-space model estimation requires only simple regression, and state order selection involves the optimal Akaike information criterion procedure. The CVA method extends to time-varying and abruptly changing systems. A reduce-rank stochastic model predictive control problem is shown to be equivalent to the CVA problem. Also discussed are computational aspects, applications, and an example illustrating the method. New extensions to the identification of general nonlinear systems are briefly discussed. The CVA method provides an approach giving reliable, automatic implementation of identification, filtering, and control for online adaptive control.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Protection conferred on mice by combinations of monoclonal antibodies directed against outer-membrane proteins or smooth lipopolysaccharide of Brucella (1992)

The effect of monoclonal antibodies (MAbs) injected alone or in combination on brucella splenic infection in CD-1 mice was tested 7 and 21 days after a challenge with virulent Brucella abortus 544. Passive immunisation of mice with anti-25-27-kDa MAb alone, or mixed with protective anti-16.5 and anti-36-38-kDa MAbs, or with MAbs of the same specificity which were previously demonstrated to have no activity on CD-1 mice, produced a significant reduction of spleen counts of B. abortus (p less than 0.01). Other combinations of MAbs did not reduce splenic infection in comparison with the untreated control group. BALB/c mice were used to test the possible interference of the immune response of CD-1 mice against MAbs that were produced in BALB/c mice. No reduction of splenic infection was shown with anti-25-27- or -36-38-kDa MAbs, whereas anti-lipopolysaccharide (LPS) MAb which was produced in CBA mice was effective. Combination of anti-protein MAbs with the anti-LPS MAb produced only the effect of the anti-LPS MAb at 7 and 21 days after challenge.
Cytophobic Surface Modification of Microfluidic Arrays for In Situ Parallel Peptide Synthesis and Cell Adhesion Assays (2007)

A combination of PEG-based surface passivation techniques and spatially addressable SPPS (solid phase peptide synthesis) was used to demonstrate a highly specific cell-peptide adhesion assay on a microfluidic platform.The surface of a silicon-glass microchip was modified to form a mixed selfassembled monolayer that presented PEG moieties interspersed with reactive amino terminals.The PEG provided biomolecular inertness and the reactive amino groups were used for consequent peptide synthesis.The cytophobicity of the surface was characterized by on-chip fluorescent binding assays and was found to be resistant to non-specific attachment of cells and proteins.An integrated system for parallel peptide synthesis on this reactive amino surface was developed, using photogenerated acid chemistry and digital microlithography.A constant synthesis efficiency of >98% was observed for up to 7mer peptides.To demonstrate specific cell adhesion on these synthetic peptide arrays, variations of a 7mer cell binding peptide that binds to murine B lymphoma cells were synthesized.Sequence specific binding was observed on incubation with fluorescently labeled, intact murine B lymphoma cells and key residues for binding were identified by deletional analysis.The benefits of miniaturized assays on microfluidic devices are numerous but have not yet been widely applied to protein and cell based assays.In general, these assays require inert
CaBP2 is a rat homolog of ERp72 with proteindisulfide isomerase activity (1993)

Ca-binding protein 2 (CaBP2) has been described previously as an intracisternal calcium-binding microsomal glycoprotein. We report now the primary sequence of this protein as deduced from the corresponding cDNA. The protein possesses a C-terminal -KEEL retention sequence and three repeats of the thioredoxin-like motive -EFYAPNCGHCK-, and represents the rat homolog of ERp72. In contrast to earlier reports on ERp72, CaBP2 possesses significant proteindisulfide isomerase activity. Furthermore, in contrast to ERp72, CaBP2 is a glycoporotein containing O-linked glycans. The amount of CaBP2 in H-35 Reuber hepatoma cells increases in parallel with that of immunoglobin heavy-chain-binding protein under conditions which lead to impaired glycosylation, while the amount of calreticulin, another KDEL-containing glycoprotein, remains almost unchanged.