gebhart/patrepeval huggingface dataset:
Whisker-reinforced bioactive composites containing calcium phosphate cement fillers: Effects of filler ratio and surface treatments on mechanical properties (2001)

Calcium phosphate cement (CPC) sets to form microporous solid hydroxyapatite with excellent osteoconductivity, but its brittleness and low strength prohibit use in stress-bearing locations. The aim of this study was to incorporate prehardened CPC particles and ceramic whiskers in a resin matrix to improve the strength and fracture resistance, and to investigate the effects of key microstructural variables on composite mechanical properties. Two types of whiskers were used: silicon nitride, and silicon carbide. The whiskers were surface-treated by fusing with silica and by silanization. The CPC particle fillers were either silanized or not silanized. Seven mass ratios of whisker-silica/CPC were mixed: 0:1 (no whisker-silica), 1:5, 1:2, 1:1, 2:1, 5:1, and 1:0 (no CPC). Each powder was blended with a bisphenol-a-glycidyl methacrylate-based resin to harden in 2 x 2 x 25 mm molds by two-part chemical curing. The specimens were tested in three-point flexure to measure strength, work-of-fracture (toughness), and elastic modulus. Two-way analysis of variance was used to analyze the data, and scanning electron microscopy was used to examine specimen fracture surfaces. The whisker-silica/CPC ratio had significant effects on composite properties (p < 0.001). When this ratio was increased from 0:1 to 1:0, the strength was increased by about three times, work-of-fracture by five times, and modulus by two times. Whisker surface treatments and CPC filler silanization also had significant effects (p < 0.001) on composite properties. Scanning electron microscopy revealed rough fracture surfaces for the whisker composites with steps and whisker pullout. Resin remnants were observed on the surfaces of the pulled-out whiskers, indicating strong whisker-matrix bonding. In conclusion, incorporating highly osteoconductive CPC fillers and ceramic whiskers yielded composites with substantially improved mechanical properties compared with composites filled with CPC particles without whiskers. The composite properties were determined by whisker-to-CPC ratio and filler surface treatments.
Selective induction of brain type II Na+ channels by nerve growth factor. (1988)

Cells derived from a rat pheochromocytoma (PC12 cells) can generate an action potential only upon treatment with nerve growth factor. Using electrophysiological methods, we found that the appearance of action potentials in nerve growth factor-treated PC12 cells can be explained by an increase in the density of Na+ channels. The functional properties of Na+ channels in PC12 cells are similar to those described for peripheral nerves but appear to be different from Na+ channels synthesized in Xenopus oocytes injected with brain type II Na+ -channel mRNA. To determine if PC12 cells express the brain type II Na+ -channel gene, we performed RNase-protection analyses using probes that can distinguish between the brain type I and type II Na+ -channel mRNAs. The results from these studies indicate that undifferentiated PC12 cells express the type II but not the type I Na+ -channel gene. Treatment with nerve growth factor increases expression of the type II Na+ -channel gene but has no effect on type I gene expression. Our findings suggest that Na+ -channel excitability in PC12 cells is due to the specific induction of the brain type II gene by nerve growth factor.
Diflunisal Analogues Stabilize the Native State of Transthyretin. Potent Inhibition of Amyloidogenesis (2003)

Analogues of diflunisal, an FDA-approved nonsteroidal antiinflammatory drug (NSAID), were synthesized and evaluated as inhibitors of transthyretin (TTR) aggregation, including amyloid fibril formation. High inhibitory activity was observed for 26 of the compounds. Of those, eight exhibited excellent binding selectivity for TTR in human plasma (binding stoichiometry >0.50, with a theoretical maximum of 2.0 inhibitors bound per TTR tetramer). Biophysical studies reveal that these eight inhibitors dramatically slow tetramer dissociation (the rate-determining step of amyloidogenesis) over a duration of 168 h. This appears to be achieved through ground-state stabilization, which raises the kinetic barrier for tetramer dissociation. Kinetic stabilization of WT TTR by these eight inhibitors is further substantiated by the decreasing rate of amyloid fibril formation as a function of increasing inhibitor concentration (pH 4.4). X-ray cocrystal structures of the TTR.18(2) and TTR.20(2) complexes reveal that 18 and 20 bind in opposite orientations in the TTR binding site. Moving the fluorines from the meta positions in 18 to the ortho positions in 20 reverses the binding orientation, allowing the hydrophilic aromatic ring of 20 to orient in the outer binding pocket where the carboxylate engages in favorable electrostatic interactions with the epsilon-ammonium groups of Lys 15 and 15'. The hydrophilic aryl ring of 18 occupies the inner binding pocket, with the carboxylate positioned to hydrogen bond to the serine 117 and 117' residues. Diflunisal itself appears to occupy both orientations based on the electron density in the TTR.1(2) structure. Structure-activity relationships reveal that para-carboxylate substitution on the hydrophilic ring and dihalogen substitution on the hydrophobic ring afford the most active TTR amyloid inhibitors.
Fiber optic detector for immuno-testing (2023)

A portable fiber optic detector that senses the presence of specific target chemicals in air or a gas by exchanging the target chemical for a fluoroescently-tagged antigen that is bound to an antibody which is in turn attached to an optical fiber. Replacing the fluorescently-tagged antigen reduces the fluorescence so that a photon sensing detector records the reduced light level and activates an appropriate alarm or indicator.
Lung Fibrosis Induced by Silica Particles in NMRI Mice Is Associated with an Upregulation of the p40 Subunit of Interleukin-12 and Th-2 Manifestations (1999)

Interleukin (IL)-12 is a cytokine produced principally by activated macrophages which is involved in control of the T-helper 1/T-helper 2 cell (Th1/Th2) polarization of immune responses. To examine its potential involvement in the development of lung fibrosis, we examined the expression (protein, messenger RNA [mRNA]) of IL-12 (p70) and of its subunits (p40 and p35) in lung homogenates, bronchoalveolar lavage fluid (BALF), and bronchoalveolar lavage (BAL) cell cultures in mouse models of resolutive alveolitis (RA) and fibrosing alveolitis (FA) induced by inorganic particles (manganese dioxide [MnO2] and crystalline silica, respectively). The administration of tungsten carbide (WC), which behaved as an innocuous dust for the lung, served as a negative control condition. The FA was specifically accompanied by a Th2-like polarization characterized by high levels of immunoglobulin (Ig)G1 in BALF and by a protracted overproduction of both p40 protein and mRNA, but not by the biologically active form of IL-12 (p70). In the RA model, the p40 response was only transient, and a Th1-like response was reflected by increased levels of interferon (IFN)-gamma and dominant levels of IgG2a in BALF. Taken together, these findings suggest that production of the p40 subunit of IL-12 and Th2 polarization play important roles in lung inflammatory and fibrotic responses to inhaled inorganic particles.
Learning Control of Robot Manipulators (1993)

Learning control encompasses a class of control algorithms for programmable machines such as robots which attain, through an iterative process, the motor dexterity that enables the machine to execute complex tasks. In this paper we discuss the use of function identification and adaptive control algorithms in learning controllers for robot manipulators. In particular, we discuss the similarities and differences between betterment learning schemes, repetitive controllers and adaptive learning schemes based on integral transforms. The stability and convergence properties of adaptive learning algorithms based on integral transforms are highlighted and experimental results illustrating some of these properties are presented.
Unmasking Electronic Energy Transfer of Conjugated Polymers by Suppression of O <sub>2</sub> Quenching (2000)

The photochemistry of poly[2-methoxy, 5-(2'-ethyl-hexyloxy)-p-phenylene-vinylene] (MEH-PPV) has been found to be highly dependent on the presence of O(2), which increases singlet exciton quenching dramatically. Spectroscopy on isolated single molecules of MEH-PPV in polycarbonate films that exclude O(2) reveals two distinct polymer conformations with fluorescence maxima near 555 and 580 nanometers wavelength, respectively. Time-resolved single-molecule data demonstrate that the 580-nanometer conformation exhibits a "landscape" for intramolecular electronic energy relaxation with a "funnel" that contains a 580-nanometer singlet exciton trap at the bottom. The exciton traps can be converted to exciton quenchers by reaction with O(2). Conformationally induced, directed-energy transfer is arguably a critical dynamical process that is responsible for many of the distinctive photophysical properties of conjugated polymers.
Identification and characterization of IS <i>1138</i>, a transposable element from <i>Mycoplasma pulmonis</i> that belongs to the <i>IS3</i> family (1993)

Insertion sequence (IS) elements are mobile genetic elements found in prokaryotes. We have identified a repetitive element from Mycoplasma pulmonis, a murine pathogen, that is similar to eubacterial IS elements. By subcloning a single strain of M. pulmonis, we isolated a variant clone in which the IS element had undergone an apparent transposition event. The nucleotide sequences of the element, designated IS1138, and the target site into which it inserted were determined. IS1138 consists of 1288 bp with 18 bp perfect terminal inverted repeats. Sequence analysis of the target site before and after insertion of IS1138 identified a 3 bp duplication of target DNA flanking the element. The predicted amino acids encoded by the major open reading frame of IS1138 share significant similarity with the transposases of the IS3 family. Southern hybridization analysis indicates that repetitive sequences similar to IS1138 are present in most, if not all, strains of M. pulmonis, but IS1138-like sequences were not detected in other mycoplasmal species.
New Analogues of Mycophenolic Acid (2016)

In this review article, the authors present the synthesis of novel derivatives of mycophenolic acid, together with their initial biological investigations.
Automating Test-Suite Augmentation (2014)

Test suites are hardly ever created from scratch. Hence, automatic test case generation methods should take advantage of existing tests to produce high-quality test suites more efficiently. We present an approach for automatic test suite augmentation addressing this challenge. It modifies existing test cases in such a way that full branch coverage in specified target functions is achieved. It is based on symbolic execution and model checking, and has been implemented as an extension to FShell, a test case generation tool for C programs. Finally, we present a case study where we apply our tool to augment a model-based test suite for real industrial code of a Java Card applet firewall, ultimately achieving 100% branch coverage fully automatically.
Harnessing the Power of Real‐World Evidence (RWE): A Checklist to Ensure Regulatory‐Grade Data Quality (2017)

The role of real-world evidence (RWE) in regulatory, drug development, and healthcare decision-making is rapidly expanding. Recent advances have increased the complexity of cancer care and widened the gap between randomized clinical trial (RCT) results and the evidence needed for real-world clinical decisions. Instead of remaining invisible, data from the >95% of cancer patients treated outside of clinical trials can help fill this void.
Prolymphocytic Leukaemia (1974)

S ummary . The clinical and haematological features of 15 patients with a rare variant of chronic lymphocytic leukaemia (CLL) are described. The disease predominantly affects males in the sixth and seventh decades of life and presenting symptoms include fatigue, weakness, weight loss, sweats and fevers. Massive enlargement of the spleen (mean weight at autopsy 1383 g, range 227–3500 g) and to a lesser extent of the liver (mean weight 2445 g, range 2030–3079 g) are regular findings. In contrast, peripheral lymphadenopathy is inconspicuous or absent. The characteristic cell in the peripheral blood is a relatively large lymphoid cell with a large vesicular nucleolus, relatively well‐condensed nuclear chromatin and moderate amount of cytoplasm. The counts of these cells in the peripheral blood at the time of diagnosis are very high (mean 355 000/μl, range 26000–1 11 000/μl). The clinical response to methods of treatment that are usually effective in classical CLL (particularly alkylating agents and corticosteroid drugs) is uniformly poor and the patients’survival after diagnosis is in most cases quite short. We believe that the clinical and haematological features of this condition justify its recognition separately from classical CLL, from lymphosarcoma cell leukaemia and from acute lymphoblastic leukaemia. We have therefore designated it ‘prolymphocytic leukaemia’. On the basis of a single case we suggest that further trials of splenectomy are indicated.
The use of optical Fourier transforms to diagnose pleomorphism, size and chromatin clumping in nuclear models. (1981)

Nuclear features of size, pleomorphism and chromatin clumping are distinctive and quantifiable in the optical Fourier transform of the nucleus. This is shown in photographs of models, tracings and hematoxylin-stained impression smears of various types of nuclei. The size of the transform is inversely proportional to the size of the nucleus. Irregularities in shape (pleomorphism) produce radially symmetric rays of light in the transform. The effect of chromatin clumping is to give a mottled pattern superimposed on the pleomorphism pattern. The transform features can be measured in real time using a ring-wedge or similar detector. These procedures might be used for both automatic screening and objective quantification of diagnoses.
Uniformity Control of Ni Thin-Film Microstructures Deposited by Through-Mask Plating (2004)

The thickness uniformity within a specimen and the cross-sectional profiles of electroplated individual Ni microstructures have been investigated as a function of the electroplating conditions. It was found that the uniformity and profiles of microstructures could be controlled by varying the process conditions. A uniform thickness distribution and microstructures with flat profiles could be obtained at optimal plating conditions of 8 mA/cm2 and 60°C. Above this optimal plating current density, the microstructure has a rabbit-ears profile, and the thickness of a narrow microstructure is thicker than that of wide ones. Below this current density, the microstructure has a cap-like cross-sectional profile, and a narrow structure is thinner than wide ones. Increasing the plating temperature enhances the nonuniformity, whereas other process parameters have insignificant effects on it. The current crowding observed in patterned specimens is responsible for the rabbit-ears profile of individual microstructures, while a combination of the fluidic friction on the sidewall of the photoresist and the electrophoresis of the ions in the solution are believed to be responsible for the abnormal cap-like profile of individual microstructures and the thinning effect on narrow microstructures.
Isolation and reactions of .alpha.-lithio N,N-dimethylacetamide (1977)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTIsolation and reactions of .alpha.-lithio N,N-dimethylacetamideRichard P. Woodbury and Michael W. RathkeCite this: J. Org. Chem. 1977, 42, 10, 1688–1690Publication Date (Print):May 1, 1977Publication History Published online1 May 2002Published inissue 1 May 1977https://pubs.acs.org/doi/10.1021/jo00430a004https://doi.org/10.1021/jo00430a004research-articleACS PublicationsRequest reuse permissionsArticle Views427Altmetric-Citations59LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
From arginine methylation to ADMA: A novel mechanism with therapeutic potential in chronic lung diseases (2009)

Protein arginine methylation is a novel posttranslational modification regulating a diversity of cellular processes, including protein-protein interaction, signal transduction, or histone function. It has recently been shown to be dysregulated in chronic renal, vascular, and pulmonary diseases, and metabolic products originating from protein arginine methylation have been suggested to serve as biomarkers in cardiovascular and pulmonary diseases. Protein arginine methylation is performed by a class of enzymes called protein arginine methyltransferases (PRMT), which specifically methylate protein-incorporated arginine residues to generate protein-incorporated monomethylarginine (MMA), symmetric dimethylarginine (SDMA), or asymmetric dimethylarginine (ADMA). Upon proteolytic cleavage of arginine-methylated proteins, free intracellular MMA, SDMA, or ADMA is generated, which, upon secretion into the extracellular space (including plasma), directly affects the methylarginine concentration in the plasma. Free methylarginines are cleared from the body by renal excretion or hepatic metabolism. In addition, MMA and ADMA, but not SDMA, can be degraded via a class of intracellular enzymes called dimethylarginine dimethylaminohydrolases (DDAH). ADMA and MMA are endogenous inhibitors of nitric oxide synthases (NOS) and ADMA has been suggested to serve as a biomarker of endothelial dysfunction in cardiovascular diseases. This view has now been extended to the idea that, in addition to serum ADMA, the amount of free, as well as protein-incorporated, intracellular ADMA influences pulmonary cell function and determines the development of chronic lung diseases, including pulmonary arterial hypertension (PAH) or pulmonary fibrosis. This review will present and discuss the recent findings of dysregulated arginine methylation in chronic lung disease. We will highlight novel directions for future investigations evaluating the functional contribution of arginine methylation in lung homeostasis and disease with the outlook that modifying PRMT or DDAH activity presents a novel therapeutic option for the treatment of chronic lung disease.
CLASSIFICATION OF LAND COVER AND LAND USE BASED ON CONVOLUTIONAL NEURAL NETWORKS (2018)

Abstract. Land cover describes the physical material of the earth’s surface, whereas land use describes the socio-economic function of a piece of land. Land use information is typically collected in geospatial databases. As such databases become outdated quickly, an automatic update process is required. This paper presents a new approach to determine land cover and to classify land use objects based on convolutional neural networks (CNN). The input data are aerial images and derived data such as digital surface models. Firstly, we apply a CNN to determine the land cover for each pixel of the input image. We compare different CNN structures, all of them based on an encoder-decoder structure for obtaining dense class predictions. Secondly, we propose a new CNN-based methodology for the prediction of the land use label of objects from a geospatial database. In this context, we present a strategy for generating image patches of identical size from the input data, which are classified by a CNN. Again, we compare different CNN architectures. Our experiments show that an overall accuracy of up to 85.7 % and 77.4 % can be achieved for land cover and land use, respectively. The classification of land cover has a positive contribution to the classification of the land use classification.
Current developments in the genetics of Rett and Rett-like syndrome (2017)

We conclude that the Rett syndrome phenotype has a much broader underlying genetic cause and the typical phenotype overlap with other genetic disorders. For proper genetic counselling, patient perspective and treatment it is important to include both phenotype and genetic information.
A Common 936 C/T Mutation in the Gene for Vascular Endothelial Growth Factor Is Associated with Vascular Endothelial Growth Factor Plasma Levels (2000)

We have found three common mutations in the VEGF gene; one of them, a 936 C/T exchange, may be an important determinant of VEGF plasma levels.
AN MDCT-BASED PSYCHOACOUSTIC MODEL CO-PROCESSOR DESIGN FOR MPEG-2/4 AAC AUDIO ENCODER (2004)

The Psychoacoustic Model (PAM) is a very important role in MPEG-2/4 Advanced Audio Coding (AAC) encoding. It determines sound quality of a given encoder and influences a lot in computational complexity. This paper presents a new architecture design for MDCT-based PAM co-processor. This work is based on the dedicated hardware design which is different from traditional programmable approaches. Moreover, to reduce the complexity, we replace the calculations of spreading function with reduced fixed coefficients, and decrease the transform kernels from three to one unit.
A novel<i>in silico</i>framework to improve MHC-I epitopes and break the tolerance to melanoma (2017)

Tolerance toward tumor antigens, which are shared by normal tissues, have often limited the efficacy of cancer vaccines. However, wild type epitopes can be tweaked to activate cross-reactive T-cell clones, resulting in antitumor activity. The design of these analogs (i.e., heteroclitic peptides) can be difficult and time-consuming since no automated <i>in silico</i> tools are available. Hereby we describe the development of an <i>in silico</i> framework to improve the selection of heteroclitic peptides. The Epitope Discovery and Improvement System (EDIS) was first validated by studying the model antigen SIINFEKL. Based on artificial neural network (ANN) predictions, we selected two mutant analogs that are characterized by an increased MHC-I binding affinity (SIINF<b>A</b>KL) or increased TCR stimulation (SII<b>W</b>FEKL). Therapeutic vaccination using optimized peptides resulted in enhanced antitumor activity and against B16.OVA melanomas <i>in vivo</i>. The translational potential of the EDIS platform was further demonstrated by studying the melanoma-associated antigen tyrosinase related protein 2 (TRP2). Following therapeutic immunization with the EDIS-derived epitope SVYDFF<b>A</b>WL, a significant reduction in the growth of established B16.F10 tumors was observed, suggesting a break in the tolerance toward the wild type epitope. Finally, we tested a multi vaccine approach, demonstrating that combination of wild type and mutant epitopes targeting both TRP2 and OVA antigens increases the antitumor response. In conclusion, by taking advantage of available prediction servers and molecular dynamics simulations, we generated an innovative platform for studying the initial sequences and selecting lead candidates with improved immunological features. Taken together, EDIS is the first automated algorithm-driven platform to speed up the design of heteroclitic peptides that can be publicly queried online.