gebhart/patrepeval huggingface dataset:
Human Immunodeficiency Virus Type 1 Induction Mediated by Genistein Is Linked to Cell Cycle Arrest in G<sub>2</sub> (1998)

Protein tyrosine kinase (PTK) phosphorylation is involved in cellular proliferation and differentiation processes that are key factors for human immunodeficiency virus type 1 (HIV-1) regulation in infected monocytic cells. Short-term exposure of the chronically infected promyelocytic OM10 cell line with the PTK inhibitor genistein induced a dose-dependent increase in p24 antigen production in culture supernatants. This induction persisted in the presence of the reverse transcriptase inhibitor, zidovudine, and was associated with an increased transcription of HIV-1 multiply spliced and unspliced RNAs, suggesting a transcriptional mechanism targeting the integrated provirus. Genistein induced cell differentiation, apoptosis, and a G2 arrest in the OM10 cells. Cell differentiation and apoptosis were not directly involved in the observed increase in HIV-1 replication that was closely linked to genistein-induced G2 arrest. Alleviation of the G2 arrest by pentoxyfylline resulted in a concomitant reduction of HIV-1 to baseline replication. Additionally, by flow cytometry, a significant increase in the number of p24 antigen-expressing cells was observed in cells arrested in G2 compared to those located in G1 or S. Tyrosine kinase inhibition was found not to be essential for enhanced viral replication, which seemed to be related to two other properties of genistein, inhibition of topoisomerase II activity and inhibition of phosphotidylinositol turnover. These findings are consistent with the recent observation that HIV-1 Vpr induces viral replication through preventing proliferation of cells by arresting them in G2 of the cell cycle and strongly suggest that manipulation of the cell cycle plays an important role in HIV-1 pathogenesis.
Macrophage-Targeted Therapy: CD64-Based Immunotoxins for Treatment of Chronic Inflammatory Diseases (2012)

Diseases caused by chronic inflammation (e.g., arthritis, multiple sclerosis and diabetic ulcers) are multicausal, thus making treatment difficult and inefficient. Due to the age-associated nature of most of these disorders and the demographic transition towards an overall older population, efficient therapeutic intervention strategies will need to be developed in the near future. Over the past decades, elimination of activated macrophages using CD64-targeting immunotoxins has proven to be a promising way of resolving inflammation in animal models. More recent data have shown that the M1-polarized population of activated macrophages in particular is critically involved in the chronic phase. We recapitulate the latest progress in the development of IT. These have advanced from full-length antibodies, chemically coupled to bacterial toxins, into single chain variants of antibodies, genetically fused with fully human enzymes. These improvements have increased the range of possible target diseases, which now include chronic inflammatory diseases. At present there are no therapeutic strategies focusing on macrophages to treat chronic disorders. In this review, we focus on the role of different polarized macrophages and the potential of CD64-based IT to intervene in the process of chronic inflammation.
Visual Fields in 4- to 10-Year-Old Children Using Goldmann and Double-Arc Perimeters (1991)

We report the comparison of field extent measured using two kinetic perimeters: 1) a double-arc perimeter developed by van Hof-van Duin for use in infants and young children, and 2) the standard Goldmann apparatus. Targets used were the V-4-e target on the Goldmann and both 2- and 6-degree targets on the double-arc perimeter. Visual fields were measured by assessing looking responses toward targets presented in a pseudorandom order at 45 degrees, 135 degrees, 225 degrees, and 315 degrees. Fifty-six children (mean age: 6.8 years, range: 4 to 10 years) were tested, with retests undertaken in 20%. The extent of total field size was highly correlated among the three tests (P less than .001) and test-retest reliability was high for all three targets (P less than .001). Our previous observation of continued growth with age in visual field extent using the 6-degree target in 4- to 10-year-old children was confirmed and also seen with the 2-degree target in arc perimetry and with the V-4-e in the Goldmann apparatus. Kinetic perimetry using a double-arc perimeter with 2- and 6-degree target sizes appears reliable and comparable, in the four meridia tested, to conventional kinetic perimetry techniques in 4- to 10-year-old children.
A Convenient Protocol for the Synthesis of Ligands from a 4-Methyl-3,5-diacylaminophenyl Platform (2004)

The synthesis of stable and highly organized phenanthroline, terpyridine, and pyridino-oxazoline ligands bearing one or two 4-methyl-3,5-diacylaminophenyl modules equipped with two lateral dialkoxyphenyl groups has been performed using EDC.HCl and DMAP reagents in the final coupling reaction. Evidently, in the final ligands and in the solid state intermolecular hydrogen bonding maintains the coherence of the tridimensional structure as clearly evidenced by FT-IR and X-ray diffraction spectroscopy in the cases of the methoxy ligands. The supramolecular packing is also maintained by additional pi-pi stacking interactions.
The IMAP METADATA Extension (2009)

The METADATA extension to the Internet Message Access Protocol permits
clients and servers to maintain or meta on IMAP
servers. It is possible to have annotations on a per-mailbox basis or
on the server as a whole. For example, this would allow comments about
the purpose of a particular mailbox to be attached to that mailbox,
or a message of the day containing server status information to be
made available to anyone logging in to the server. Change History (to
be removed prior to publication as an RFC) Changes from -10 to -11:
1. Added new paragraph to indicate that values may be read-only or
computed. 2. /admin server annotation value now must be a URI. 3.
Clarified that SORT and THREAD extensions are not required. 4. Fixed
use of undefined entries in some examples. 5. Fixed many examples. 6.
Added IANA registration for LIST-EXTENDED items. 7. Added match type
and collation identifier to the LIST-EXTENDED selection option. 8.
Made support for IMAP-I18N a requirement. 9. Minor text clarifications
applied. 10. Remove mailbox list set atomicity requirement. 11.
Clarified that annotations can only be set on mailboxes that actually
exist. Changes from -09 to -10: 1. Updated to rfc 4466 reference. 2.
Reworded data model description. 3. Reworked LIST-EXTENDED so that
responses have metadata items after the mailbox info. 4. Various
spelling fixes. Changes from -08 to -09: 1. Remove content-language
attribute and reference. 2. Changed capability and command names. 3.
Revised abstract. Changes from -07 to -08: 1. Changed 'string'
formal syntax to 'list-mailbox' and 'astring' for
entry/attribute names. 2. Updated examples to match new astring
syntax. 3. Changed CAPABILITY name due to incompatible syntax change.
4. Removed content-type attribute. 5. Added Content-type to IANA
registration for entries. 6. Removed vendor attributes. 7. Fixed
examples in section 3.3 for multi-mailbox and multi-entry cases. 8.
Removed wildcards for attributes. 9. Entry/attributes can now only be
ASCII. 10. Tied up text wrt storing/fetching. 11. Added Conventions
section 12. Entry/attributes MUST NOT contain consecutive or trailing
'/' or '.'.
Specific binding of surfactant apoprotein SP-A to rat alveolar macrophages (1992)

Surfactant protein A (SP-A) influences the function of alveolar macrophages in vitro. In this study the characteristics of the binding of 125I-labeled SP-A to rat alveolar macrophages has been investigated. The binding of SP-A to alveolar macrophages at 4 degrees C was saturable with half-maximal binding at a SP-A concentration of 4 micrograms/ml. Bound SP-A was rapidly displaced by an excess of unlabeled SP-A. The binding of labeled SP-A to the alveolar macrophages was blocked in a dose-dependent fashion by unlabeled SP-A, the collagen-like protein C1q and type V collagen but not by bovine serum albumin. These results suggest that a component of the interaction between SP-A and alveolar macrophages is mediated through the collagen-like domain of SP-A and that the characteristics of this interaction are consistent with there being a specific receptor for SP-A on the surface of alveolar macrophages.
A Method for Generation of Bone Marrow-Derived Macrophages from Cryopreserved Mouse Bone Marrow Cells (2010)

The broad use of transgenic and gene-targeted mice has established bone marrow-derived macrophages (BMDM) as important mammalian host cells for investigation of the macrophages biology. Over the last decade, extensive research has been done to determine how to freeze and store viable hematopoietic human cells; however, there is no information regarding generation of BMDM from frozen murine bone marrow (BM) cells. Here, we establish a highly efficient protocol to freeze murine BM cells and further generate BMDM. Cryopreserved murine BM cells maintain their potential for BMDM differentiation for more than 6 years. We compared BMDM obtained from fresh and frozen BM cells and found that both are similarly able to trigger the expression of CD80 and CD86 in response to LPS or infection with the intracellular bacteria Legionella pneumophila. Additionally, BMDM obtained from fresh or frozen BM cells equally restrict or support the intracellular multiplication of pathogens such as L. pneumophila and the protozoan parasite Leishmania (L.) amazonensis. Although further investigation are required to support the use of the method for generation of dendritic cells, preliminary experiments indicate that bone marrow-derived dendritic cells can also be generated from cryopreserved BM cells. Overall, the method described and validated herein represents a technical advance as it allows ready and easy generation of BMDM from a stock of frozen BM cells.
A Chaos Theoretic Analysis of Motion and Illumination in Video Sequences (2007)

Accurate and robust image motion detection has been of substantial interest in the image processing and computer vision communities. Unfortunately, no single motion detection algorithm has been universally superior, yet biological vision systems are adept at motion detection. Recent research in neural signals have shown biological neural systems are highly responsive to signals which appear to be chaotic in nature. In this paper, we exploit these biological results and hypothesize that motion in images may produce changes in pixel amplitudes that are reminiscent of chaotic dynamical systems. In particular, we demonstrate that the trajectories of pixel amplitudes in phase space due to motion result in chaos-like behavior. We likewise demonstrate that the effects of spatio-temporally varying illumination produces phase space trajectories of the pixel amplitudes which are clearly non-chaotic. We review the research tying chaotic behavior to the fractal characteristics of phase space trajectories, and we investigate multi-fractal measures which can be used to classify the pixels in an image stream based on their fractal behavior in phase space. We finally apply these measures to the task of motion detection and segmentation and show they are effective in identifying moving objects while ignoring spatio-temporally varying illumination changes.
Humic substances in natural waters and their complexation with trace metals and radionuclides: a review. [129 references] (1985)

Dissolved humic substances (humic and fulvic acids) occur in surface waters and groundwaters in concentrations ranging from less than 1 mg(C)/L to more than 100 mg(C)/L. Humic substances are strong complexing agents for many trace metals in the environment and are also capable of forming stable soluble complexes or chelates with radionuclides. Concentrations of humic materials as low as 1 mg(C)/L can produce a detectable increase in the mobility of some actinide elements by forming soluble complexes that inhibit sorption of the radionuclides onto rock materials. The stability of trace metal- or radionuclide-organic complexes is commonly measured by an empirically determined conditional stability constant (K'), which is based on the ratio of complexed metal (radionuclide) in solution to the product concentration of uncomplexed metal and humic complexant. Larger values of stability constants indicate greater complex stability. The stability of radionuclide-organic complexes is affected both by concentration variables and envionmental factors. In general, complexing is favored by increased of radionuclide, increased pH, and decreased ionic strength. Actinide elements are generally most soluble in their higher oxidation states. Radionuclides can also form stable, insoluble complexes with humic materials that tend to reduce radionuclide mobility. These insoluble complexes may be radionuclide-humate colloids that subsequently precipitate from solution, or complexes of radionuclides and humic substances that sorb to clay minerals or other soil particulates strongly enough to immobilize the radionuclides. Colloid formation appears to be favored by increased radionuclide concentration and lowered pH; however, the conditions that favor formation of insoluble complexes that sorb to particulates are still poorly understood. 129 refs., 25 figs., 19 tabs.
Functional Foods Enriched with Marine Microalga Nannochloropsis oculata as a Source of ω-3 Fatty Acids (2014)

The demand for functional food incorporated with ω-3 fatty acids is increasing over the years due to their added health benefits, such as reducing the risk of cardiovascular diseases, type II diabetes, ocular diseases, arthritis, etc. This study mainly aims to develop functional cookies and pasta enriched with ω-3 fatty acids. Nannochloropsis oculata was used because of its relatively high growth rate, high lipid content, resistance to mixing and contamination together with high nutritional values. The effect of the incorporation of Nannochloropsis oculata biomass on colour, firmness, fatty acid profile and sensory characteristics of cookies and pasta were evaluated. The colour values were found to be stable for two months of storage and the firmness increased with the addition of microalgal biomass. Omega-3 polyunsaturated fatty acid (PUFA) levels (eicosapentaenoic and docosahexaenoic acids) of 98 mg per 100 g and 63 mg per 100 g were observed in cookies and pasta, respectively, enriched with 1 % of Nannochloropsis oculata biomass. Sensory evaluation showed that the addition of up to 2 and 3 % of microalgal biomass was positively evaluated and accepted for cookies and pasta, respectively. This study confirms that the cookies and pasta enriched with Nannochloropsis oculata biomass might be used as a potential source of ω-3 fatty acids.
Effects of post-mortem time before chilling and chilling temperatures on water-holding capacity and texture of turkey breast muscle (1997)

Two studies were conducted to determine 1) the effect of post-mortem time before chilling on hot boned prerigor breast muscle water-holding capacity (WHC), and 2) post-mortem temperature effect on sarcomere length and drip loss of uncooked breast, and shear force and WHC of turkey breast muscle that is hot-boned, marinated, and cooked. In Study 1, the turkey breast muscle was excised immediately after slaughter and chilled at 0 C. The effect of post-mortem time (15, 30, 45, 60, 90, and 120 min) before chilling on muscle pH, drip loss, and WHC (measured by homogenate cooking yield) was determined. In Study 2, hot boned prerigor breast slices were held at either 0 or 12 C and then at 3 h post-mortem stored at 2 C. Sarcomere length and drip loss of uncooked breast, and the shear force and WHC (measured by cooking yield) of cooked breast muscles with water, salt, and sodium tripolyphosphate were determined. Results indicated that pH on Day 1 post-mortem and cooking yield of water homogenates were not influenced by the post-mortem time before chilling. Drip losses increased as the post-mortem time before chilling was increased. Cooking yield of homogenate with water, salt, and sodium tripolyphosphate was decreased when breast muscle was chilled after 60 min post-mortem. Drip loss of breast was less at 0 C than at 12 C, but cooking yield and shear force were not affected by post-mortem chilling temperature. It was concluded that the post-mortem time before chilling has an influence on uncooked and cooked muscle WHC, but the differences found in uncooked muscle drip loss were not reflected in WHC or shear force of cooked muscle with water, salt, and sodium tripolyphosphate added.
Hyperspectral Image Super-Resolution With a Mosaic RGB Image (2018)

Recently, many hyperspectral (HS) image superresolution methods that merge a low spatial resolution HS image and a high spatial resolution three-channel RGB image have been proposed in spectral imaging. A largely ignored fact is that most existing commercial RGB cameras capture high resolution images by a single CCD/CMOS sensor equipped with a color filter array (CFA). In this paper, we account for the common imaging mechanism of commercial RGB cameras, and propose to use a mosaic RGB image for HS image super-resolution, which prevents demosaicing error and thus its propagation into the HS image super-resolution results. We design a proper nonlocal low-rank regularization to exploit the intrinsic properties - rich self-repeating patterns and high correlation across spectra - within HS images of natural scenes, and formulate the HS image super-resolution task into a variational optimization problem, which can be efficiently solved via the alternating direction method of multipliers (ADMM). The effectiveness of the proposed method has been evaluated on two benchmark datasets, demonstrating that the proposed method can provide substantial improvement over the current state-of-the-art HS image superresolution methods without considering the mosaicing effect. Finally, we show that our method can also perform well in the real capture system.
GEOTECHNIC ZONING OF SLOPE BY REMOTE SENSING FOR PHYSICAL ENVIRONMENT PLANNING: ANALYSIS OF THE AGRO-ENVIRONMENTAL POTENTIAL AND MECHANIZATION OF SUGARCANE (2022)

Part of the sugarcane cultivation in the state of So Paulo has a great predominance in the Administrative region of Ribeiro Preto -SP, composed of 25 municipalities in the northeast region of the state that represent 60% of the sugarcane in So Paulo. The Region has a high valuation of its lands due to the good conditions of climate, location and productivity, however, studies that address agricultural and environmental aspects in agricultural management are incipient. Thus, the objective of this study was to identify the agricultural, agroclimatic and agro-environmental potential of sugarcane in the Administrative Region of Ribeiro Preto, and the aptitude for the mechanized production system with a view to an adequate and efficient territorial planning. The region has 91.9% of its area with adequate climatic suitability and 89.3% of land suitable for mechanization, with slopes ranging from 0 to 12%. The agro-environmental zoning of adequate planting occupies 39%, but with the crossing between environmentally fragile and vulnerable zones, adequate planting followed by environmental restrictions of use extends to 44% of the areas and with environmental limitations of use in 12.5%, use inadequate extends by only 4.4%. The city of Jaboticabal has the largest area under the criteria for sugarcane cultivation, 596.5 km (84.3%). Geographic information systems are powerful tools in these analyses, assisting in the zoning and quantification of areas, allowing knowledge and assistance to managers in decision-making on land use policy.
Antibodies against<i>Citrobacter braakii</i>O37 cells recognize the<i>N</i>-glycan of the band 3 glycoprotein of human erythrocyte membrane (2008)

The phenomenon of molecular mimicry was found previously for Citrobacter braakii O37, which shared epitopes with human and horse erythrocytes. The aim of this study was to elucidate the basis of the serological cross-reactivity between anti-C. braakii O37 serum and human erythrocytes. The experiments involved analyzing the epitope on the human erythrocyte membrane, that could be recognized by affinity-purified antibodies. The results indicated a specific glycoprotein fraction in immunoblotting, namely band 3, which interacted with the antibodies purified on lipopolysaccharide from C. braakii O37 LPS (LPS O37) and its core affinity columns. Treating the erythrocytes with trypsin, which cleaves glycophorin A, improved the agglutination because band 3 became more available for antibody binding. Isolated band 3 immobilized on an affinity plate could be used to purify antibodies from the anti-C. braakii O37 serum. These antibodies showed a specific reactivity to LPS O37, but not to the related lipopolysaccharide from Salmonella Toucra O48. Furthermore, the inhibition of agglutination with lactose, the diminished interaction of the specific antibodies purified on LPS O37 with endo-beta-galactosidase-treated band 3, and the reactivity of these antibodies to the 40-kDa fragment of band 3 but not to its trypsin-elaborated 60-kDa fragment, all indicated that the epitope is located on the N-glycan of band 3.
Effect of Mask Discretization on Performance of Silicon Arrayed Waveguide Gratings (2014)

We studied the impact of the lithography mask discretization on silicon arrayed waveguide grating (AWG) performance. When we decreased the mask grid from 5 to 1 nm, we observed an experimental improvement in crosstalk of 2.7-6 dB and cumulative crosstalk improvement of 1.2-5 dB, depending on the wavelength channel spacing and the number of output channels. We demonstrate the effect for the AWGs with 200- and 400-GHz channel spacing, with 4, 8, and 16 output wavelength channels. With 1-nm mask grid, the average crosstalk is -26 and -23 dB for 400- and 200-GHz devices, respectively. This is the lowest crosstalk for silicon AWGs reported to the best of our knowledge. A simulation study is performed by looking specifically at phase errors due to mask grid snapping (ignoring other phase error sources), which shows an expected improvement in crosstalk of 12 dB.
Reactive power loading of components within a modular multi-level HVDC VSC converter (2011)

The modular multi-level (MMC) HVDC VSC converter topology is becoming widely accepted as the de facto standard topology. Whilst it is normal to consider primarily the real power capability of such a converter it is worth bearing in mind that the origins of the circuit application to AC power systems was in STATCOM applications. This paper will look at the basic main circuit components of a modular multi-level converter and show how the real and reactive capabilities of these components are not symmetrical. These capabilities potentially offer additional rating, even on a temporary basis from the equipment. The paper will further look at the power electronic converter and compare two different types of multi-module converter, showing how the topology affects the reactive power capability of the converter.