gebhart/patrepeval huggingface dataset:
Promoter exchange between ompF and ompC, genes for osmoregulated major outer membrane proteins of Escherichia coli K-12 (1984)

Expression of the ompF and ompC genes coding for major outer membrane proteins OmpF and OmpC is regulated in opposite directions by medium osmolarity. Chimera genes were constructed by a reciprocal exchange of the promoter-signal sequence region between the two genes. The chimera gene construction was designed so that the proteins synthesized by these genes were essentially the same as the OmpC and OmpF proteins. Studies with the chimera genes demonstrated that the osmoregulation of the OmpF-OmpC synthesis was promoter dependent. They also showed that cells grew normally even when the osmoregulation took place in opposite directions. The effects of the ompR2 and envZ mutations, which suppress ompC and ompF expression, respectively, also became reversed. The reduced expression was still subject to the promoter-controlled osmoregulation. Based on these observations, the mechanism of regulation of the ompF-ompC gene expression and its physiological importance are discussed.
Sentiment Aggregation using ConceptNet Ontology (2013)

Sentiment analysis of reviews traditionally ig-nored the association between the features of the given product domain. The hierarchical re-lationship between the features of a product and their associated sentiment that influence the polarity of a review is not dealt with very well. In this work, we analyze the influence of the hierarchical relationship between the prod-uct attributes and their sentiments on the over-all review polarity. ConceptNet is used to automatically create a product specific ontol-ogy that depicts the hierarchical relationship between the product attributes. The ontology tree is annotated with feature-specific polari-ties which are aggregated bottom-up, exploit-ing the ontological information, to find the overall review polarity. We propose a weakly supervised system that achieves a reasonable performance improvement over the baseline without requiring any tagged training data. 1
Dienstleistungsmanagement und Dienstleistungen (2011)

Im Hinblick auf das Management von Dienstleistungen existieren zwei wesentliche Literaturströme. Zum einen erfolgt die Betrachtung von Dienstleistungen aus der Marketingperspektive. Zum anderen werden Services unter operativen Gesichtspunkten beurteilt. Beide Literaturströme beziehen sich stets auf die Andersartigkeit von Dienstleistungen im Vergleich zu Produkten und umfassen die hieraus resultierenden Besonderheiten für das Management von Dienstleistungen. Ziel dieses Kapitels ist, zum einen die wesentlichen Eigenschaften, die eine Dienstleistung ausmachen, zu identifizieren sowie zum anderen die auf diesen dienstleistungsinhärenten Eigenschaften basierenden Herausforderungen im Managementkontext aufzuzeigen. Die hieraus resultierenden Erkenntnisse finden dann bei der späteren Modellgestaltung in der vorliegenden Arbeit Anwendung.
Inflammation of different tissues in spontaneously hypertensive rats. (2006)

The hypertension is one of chronic vascular diseases, which often implicates multiple tissues causing stroke, cardiac hypertrophy, and renal failure. A growing body of evidence suggests that inflammatory mechanisms are important participants in the pathophysiology of hypertension. In this study, the inflammatory status of these tissues (kidney, liver, heart, and brain) in spontaneously hypertensive rats (SHR) was analyzed and its molecular mechanism was explored. The tissues were dissected from SHR and age-matched control Wistar-Kyoto (WKY) rats to investigate the abundance of inflammation-related mediators (IL-1beta, TNFalpha, ICAM-1, iNOS, C/EBPdelta and PPARgamma). mRNA levels were determined by reverse transcription-polymerase chain reaction and protein expression was evaluated by Western blot. To evaluate the oxidative stress of tissues, carbonyl protein content and total antioxidant capacity of tissues were detected by spectrophotometry and ferric reduction ability power (FRAP) method. The results suggest that: (1) Expressions of inflammation-related mediators (IL-1beta, TNFalpha, ICAM-1, iNOS, C/EBPdelta and PPARgamma) in SHR were higher compared with those in WKY rats except no evident increase of IL-1beta mRNA in liver and brain in SHR. (2) Tissues in SHR contained obviously increased carbonyl protein (nmol/mg protein) compared to that in WKY rats (8.93+/-1.08 vs 2.27+/-0.43 for kidney, 2.23+/-0.23 vs 0.17+/-0.02 for heart, 13.42+/-1.10 vs 5.72+/-1.01 for brain, respectively, P<0.05). However, no evident difference in the amount of carbonyl protein in liver was detected between SHR and WKY rats. (3) Total antioxidant capacities of kidney, liver, heart and brain were markedly lower in SHR than that in WKY rats (P<0.05). Thus, the present data reveal a higher inflammatory status in the important tissues in SHR and indicate that inflammation might play a potential role in pathogenesis of hypertension and secondary organ complications.
Inheritance of Phytophthora Stem Blight Resistance as Compared to Phytophthora Root Rot and Phytophthora Foliar Blight Resistance in Capsicum annuum L. (2005)

The pathogen Phytophthora capsici Leon. is known to be a limiting factor of chile pepper ( Capsicum L.) production around the world. The genetics of the resistance is becoming better understood due to the specific nature of the host-pathogen interaction, i.e., all plant organs are subject to infection. It has been shown that phytophthora root rot resistance and phytophthora foliar blight resistance are under different genetic mechanisms. This study aimed at understanding the inheritance of resistance of phytophthora stem blight and to determine whether phytophthora stem blight was the same disease syndrome as phytophthora root rot and phytophthora foliar blight. Stem cuttings of a segregating F 2 population and testcross progeny facilitated the ability to screen for two disease syndromes concurrently. When the three disease syndromes were compared separately, the F 2 populations fit a 3 resistant (R): 1 susceptible (S) ratio and the testcross progenies fit a 1R:1S ratio. When comparative studies were performed (stem vs. foliar and stem vs. root), the F 2 populations fit a 9R/R:3R/S:3S/R:1S/S ratio and the testcross fit a 1R/R:1R/S:1S/R:1S/S ratio. These ratios are consistent of a single gene controlling the resistance of each system. Therefore, phytophthora stem blight, root rot, and foliar blight are three separate disease syndromes.
Isolated WDDL (2009)

Security protocols are frequently accelerated by implementing the underlying cryptographic functions in reconfigurable hardware. However, unprotected hardware implementations are susceptible to side-channel attacks, and Differential Power Analysis (DPA) has been shown to be especially powerful. In this work, we evaluate and compare the effectiveness of common hiding countermeasures against DPA in FPGA-based designs, using the Whirlpool hash function as a case study. In particular, we develop a new design flow called Isolated WDDL (IWDDL). In contrast with previous works, IWDDL isolates the direct and complementary circuit paths, and also provides DPA resistance in the Hamming distance power model. The analysis is supported using actual implementation results.
Adhesion-independent mechanism for suppression of tumor cell invasion by E-cadherin (2003)

Loss of E-cadherin expression or function in tumors leads to a more invasive phenotype. In this study, we investigated whether the invasion suppressor activity of E-cadherin is mediated directly by tighter physical cell adhesion, indirectly by sequestering beta-catenin and thus antagonizing beta-catenin/T cell factor (TCF) signaling, or by other signaling pathways. To distinguish mechanisms, we expressed wild-type E-cadherin and various E-cadherin mutants in invasive E-cadherin-negative human breast (MDA-MB-231) and prostate (TSU-Pr1) epithelial carcinoma cell lines using a tetracycline-inducible system. Our data confirm that E-cadherin inhibits human mammary and prostate tumor cell invasion. We find that adhesion is neither necessary nor sufficient for suppressing cancer invasion. Rather, the invasion suppressor signal is mediated through the beta-catenin-binding domain of the E-cadherin cytoplasmic tail but not through the p120ctn-binding domain. beta-catenin depletion also results in invasion suppression. However, alteration in the beta-catenin/TCF transcriptional regulation of target genes is not required for the invasion suppressor activity of E-cadherin, suggesting the involvement of other beta-catenin-binding proteins.
Extracellular matrix rigidity governs smooth muscle cell motility in a biphasic fashion (2005)

Increasing evidence suggests that mechanical cues inherent to the extracellular matrix (ECM) may be equally as critical as its chemical identity in regulating cell behavior. We hypothesized that the mechanical properties of the ECM directly regulate the motility of vascular smooth muscle cells (SMCs) and tested this hypothesis using polyacrylamide substrates with tunable mechanical properties. Quantification of the migration speed on uniformly compliant hydrogels spanning a range of stiffnesses (Young's moduli values from 1.0 to 308 kPa for acrylamide/bisacrylamide ratios between 5/0.1% and 15/1.2%, respectively) revealed a biphasic dependence on substrate compliance, suggesting the existence of an optimal substrate stiffness capable of supporting maximal migration. The value of this optimal stiffness shifted depending on the concentration of ECM protein covalently attached to the substrate. Specifically, on substrates presenting a theoretical density of 0.8 microg/cm(2) fibronectin, the maximum speed of 0.74 +/- 0.09 microm/min was achieved on a 51.9 kPa gel; on substrates presenting a theoretical density of 8.0 microg/cm(2) fibronectin, the maximum speed of 0.72 +/- 0.06 microm/min occurred on a softer 21.6 kPa gel. Pre-treatment of cells with Y27632, an inhibitor of the Rho/Rho-kinase (ROCK) pathway, reduced these observed maxima to values comparable to those on non-optimal stiffnesses. In parallel, quantification of TritonX-insoluble vinculin via Western blotting, coupled with qualitative fluorescent microscopy, revealed that the formation of focal adhesions and actin stress fibers also depends on ECM stiffness. Combined, these data suggest that the mechanical properties of the underlying ECM regulate Rho-mediated contractility in SMCs by disrupting a presumptive cell-ECM force balance, which in turn regulates cytoskeletal assembly and ultimately, cell migration.
Human Papilloma Virus 16 E6 Oncoprotein Inhibits Retinoic X Receptor-mediated Transactivation by Targeting Human ADA3 Coactivator (2002)

The expression of human papillomavirus (HPV) E6 oncoprotein is causally linked to high-risk HPV-associated human cancers. We have recently isolated hADA3, the human homologue of yeast transcriptional co-activator yADA3, as a novel E6 target. Human ADA3 binds to the high-risk (cancer-associated) but not the low-risk HPV E6 proteins and to immortalization-competent but not to immortalization-defective HPV16 E6 mutants, suggesting a role for the perturbation of hADA3 function in E6-mediated oncogenesis. We demonstrate here that hADA3 directly binds to the retinoic X receptor (RXR)alpha in vitro and in vivo. Using chromatin immunoprecipitation, we show that hADA3 is part of activator complexes bound to the native RXR response elements within the promoter of the cyclin-dependent kinase inhibitor gene p21. We show that hADA3 enhances the RXR(alpha)-mediated sequence-specific transactivation of retinoid target genes, cellular retinoic acid-binding protein II and p21. Significantly, we demonstrate that E6 inhibits the RXR(alpha)-mediated transactivation of target genes, implying that perturbation of RXR-mediated transactivation by E6 could contribute to HPV oncogenesis.
Ion Exchange Resins for Ophthalmic Delivery (1994)

A new novel delivery system for ophthalmic drugs was developed using an antiglaucoma agent Betaxolol Hydrochloride as a model. The new delivery system involved both the binding and release of drug from ion exchange resin particles. Betaxolol was studied in-vitro via a release model analysis. The ocular comfort of Betaxolol was greatly enhanced by reducing the availability of free drug molecules in the precorneal tear film. The amount of resin concentration was selected to obtain optimum binding of the drug. The zeta potential of suspended particles was adjusted to produce flocculated suspension. Drug resin particles were then incorporated into the structured vehicle, containing Carbomer 934P as a polymer, to enhance the physical stability and ease of resuspendability of the product. This delivery system also optimized the bioavailability of Betaxolol, reducing the total drug concentration in half to 0.25% Betaxolol in 0.25% BETOPTIC S Ophthalmic Suspension as compared with 0.5% Betaxolol in BETOPTIC 0.5% Sterile Ophthalmic Solution dosage form. Increased comfort of 0.25% BETOPTIC S Ophthalmic Suspension, as well as its bioequivalency data in animal models (rabbits), was confirmed in actual clinical trials of the product 0.25% BETOPTIC S Ophthalmic Suspension. The 0.25% BETOPTIC S Ophthalmic Suspension product has been approved gamma FDA and is marketed in U. S. since February 1990. The 0.25% BETOPTIC S Ophthalmic Suspension formulation has an increased bioavailability (equivalent to BETOPTIC 0.5% Sterile Ophthalmic Solution at half the concentration of drug); and pharmaceutically, is an elegant suspension product which settles slowly providing uniform dosage and increased ocular comfort.
The myeloma landscape in 2008. (2008)

Highlights From the American Society of Hematology 2007 Annual Meeting, Atlanta, Georgia, December 8-11, 2007.
Upgrade to the K-band uplink channel for the ESA Deep Space Antennas: Analysis of the optics and preliminary dichroic mirror design (2020)

The incoming planned missions to the Moon and beyond would require an uplink channel significantly larger in frequency than the ones normally used in S and X band. This paper presents a preliminary study for the upgrade of the European Space Agency (ESA) Deep Space Antennas (DSAs) optical configuration in order to implement the new K band uplink channel (22.55–23.15 GHz). Among the different possible Beam Waveguide (BWG) layouts, one of the many aspect to be investigated is the design of new Frequency Selective Surfaces (FSSs), which are employed to merge/separate the different frequency bands. Preliminary designs and results, for both the BWG optics and the dichroic mirrors, are presented, with a particular focus on the advantages and disadvantages of the different solutions.
First demonstration of all-optical QPSK signal regeneration in a novel multi-format phase sensitive amplifier (2010)

We propose a novel black-box optical phase sensitive amplifier (PSA) configuration and describe its application to the regeneration of multi-level phase encoded signals. The concept is demonstrated with a 10 Gbaud quadrature phase shift keyed (QPSK) input.
Towards speech rate independence in large vocabulary continuous speech recognition (2002)

We present a new speech rate classifier (SRC) which is directly based on the dynamic coefficients of the feature vectors and it is suitable to be used in real time. We also report the study that has been carried out to determine what parameters of speech are the best regarding the speech rate classification problem. In this study we analyse the correlation between several speech parameters and the average speech rate of the utterance. Finally, we report a compensation technique, which is used together with the SRC. This technique provides with a word error rate (WER) reduction of a 64.1% for slow speech rate and a 32% reduction of the average WER.
Copper-catalyzed direct oxidative annulation of N-iminopyridinium ylides with terminal alkynes using O<sub>2</sub>as oxidant (2012)

The aerobic direct dehydrogenative annulation of N-iminopyridinium ylides with terminal alkynes leading to pyrazolo[1,5-a]pyridine derivatives has been developed.
Capacitive-based dilatometer cell constructed of fused quartz for measuring the thermal expansion of solids (2008)

A dilatometer cell that can detect sub angstrom changes in the length of solid specimens in the temperature range 5 K<T<350 K is described. It is constructed entirely from fused quartz (silica). This is a capacitive-based technique, where the spacing between two metallized plates allows detection of changes in length. It is also a relative measurement in the sense that the capacitor plate spacing is determined by the relative thermal expansions of the cell and sample. The advantage of using fused quartz is that it has among the smallest known thermal expansions. As a result, for most measurements, the sample's thermal expansion dominates the capacitance change. The construction and performance are described. It is directly compared to a dilatometer cell constructed of copper. Some thermometry and temperature control issues specific to dilatometry measurements are discussed.
ANEMONA: A language for programming NEtwork MONitoring Applications. (2003)

We present ANEMONA: a language for distributed network management programming. The compilation of an ANEMONA program generates code for configuring DisMan MIB’s thus automating a complex task that is prone to errors if done manually. The language allows the definition of expressions of managed objects that are monitored, as well as triggers that when fired may indicate the occurrence of associated events, which are also defined by the language. A translator for the language was implemented that generates code for configuring the Expression MIB and the Event MIB. Experimental results are presented, including an ANEMONA program that detects TCP Syn Flooding attacks, and a program for detecting steep variations on the utilization of monitored links.
Novel Compact Branch-Line Coupler Using Non-Uniform Folded Transmission Line and Shunt Step Impedance Stub With Harmonics Suppressions (2016)

In this paper, a novel compact branch-line coupler (BLC) using non-uniform folded transmission line (NUFTL) and shunt step impedance stubs (SSISs) with harmonics suppressions is presented and its design procedure is discussed. In the proposed structure, in order to reject the unwanted harmonic pass bands, a pair of SSISs is implemented along the ports of the coupler. To minimize the physical size of the coupler, NUFTL are used instead of uniform transmission lines in each arm of the branch-line. With the proposed approach, the size of the quarter-wavelength transmission line in the branch-line can be reduced greatly. The presented BLC has a compact size while showing good return loss and insertion loss characteristic in the frequency band of interest. Good agreement exists between the simulated and measured results.
Adaptive control and optimization of electromagnetic radiation, attenuation, and scattering using self-adaptive material systems (2003)

Adaptive control of radiation and scattering by integrated antenna arrays in the long-wavelength limit is investigated. A hybrid active/passive system with embedded control logic is considered and analyzed as a synthetic composite medium with adaptive constitutive parameters. Optimal control techniques yield the transfer function of the controller for a given internal or external stimulus and cost function. The method is applied to the minimization of radiated or scattered power at large distance. A limiting condition for omnidirectional power reduction is derived. A chequered planar array of interleaved subarrays for wave conditioning in reflection and transmission is analyzed in detail. Its dyadic Green function, impedance, equivalent permittivity, and reflection, transmission and radiation coefficients for general transfer functions are calculated. For random configurations, the effective permittivity and frequency response of the reflection and transmission by a free-standing slab of the self-adaptive medium are analyzed. The results may find application in the design and operation of electromagnetic smart materials.
Physics-based tracking of 3D objects in 2D image sequences (2002)

We present a new technique for tracking 3D objects in 2D image sequences. We assume that objects are constructed from a class of volumetric part primitives. The models are initially recovered using a qualitative shape recovery process. We subsequently track the objects using local forces computed from image potentials. Therefore we avoid the expensive computation of image features. By integrating measurements from stereo images, 3D positions (as well as other model parameters) of the objects can be continuously updated using an extended Kalman filter. Our model-based approach can handle occlusions in scenes with multiple moving objects by predicting their occurrences. To handle severe or unexpected occlusion we use a feedback mechanism between the quantitative and qualitative shape estimation systems. We demonstrate our technique in experiments involving image sequences from complex motions of objects.
Isolation and Identification of the Pathogens of Bullfrog Streptoccus Disease (2015)

The tested strain from typical clinical cases,and the physio-chemical characteristics,16 S rRNA gene sequence and drug sensitivity challenge property were analyzed.This isolate was determined as Streptococcus agalactiae,16 S rRNA gene fragment of 1 280 bp in length.Blast analysis showed that this fragment shared a 100% homology with the conservative region of Streptococcus agalactiae isolate(AB023574),Streptococcus dysqalactiae isolate(HM146084.1,AB537922,EU075033),Streptococcus difficilis isolate(AB112407) accessed in Genbank.The isolate was sensitive to ciprofloxacin,neomycin,spectinomycin,furadantin and enoxacin.The results lay foundation for the effective control,rational medication and vaccine preparation for Streptococcus disease on bullfrogs.
BRCA1 Zinc RING Finger Domain Disruption Alters Caspase Response in Ovarian Surface Epithelial Cells (2002)

BACKGROUND: The frequently occurring 185delAG mutation occurs in the amino-terminal zinc RING domain of the breast and ovarian cancer susceptibility gene, BRCA1. We sought to determine differential cell viability and apoptotic response of human ovarian surface epithelial cells with and without the 185delAG mutation. RESULTS: BRCA1wt and BRCA1+ cells were treated with staurosporine. Cell proliferation assays showed BRCA1wt cells grew to a greater extent compared to BRCA1+ cells. Trypan blue exclusion assays confirmed this observation. Western immunoblot analysis revealed that caspase 3 levels were higher after staurosporine treatment in BRCA1+ cells than in wild type cells, while full length DNA Fragmentation Factor 45 levels were lower in BRCA1+ cells. While there was no significant difference in levels of excision repair cross complementing protein1 (ERCC1) with BRCA1 status, BRCA1+ cells demonstrated cleavage of polyribose ADP polymerase (PARP) before wild type cells. CONCLUSIONS: Disruption of the BRCA1 RING domain caused altered cell viability and caspase-dependent apoptotic response after chemotoxic stress.
Employing Different Viewpoints for Remote Guidance in a Collaborative Augmented Environment (2018)

This paper details the design, implementation and an initial evaluation of a collaborative platform named OptoBridge, which is aimed at enhancing remote guidance and skill acquisition for spatially distributed users. OptoBridge integrates augmented reality (AR), gesture interaction with video mediated communication and is preliminarily applied to the experimental teaching of the adjustment task with Michelson interferometer. An exploratory study has been conducted to qualitatively and quantitatively evaluate the extent to which different viewpoints affect the student's sense of presence, task performance, learning outcomes and subjective feelings in the remote collaborative augmented environment. 16 students from local universities have participated in the evaluation. The result shows the influence of two different viewpoints and indicates that OptoBridge can effectively support remote guidance and enhance the collaborators' experience.