gebhart/patrepeval huggingface dataset:
Compression of time-dependent geometry (1999)

Article Free Access Share on Compression of time-dependent geometry Author: Jerome Edward Lengyel Microsoft Research, One Microsoft Way, Redmond, WA Microsoft Research, One Microsoft Way, Redmond, WAView Profile Authors Info & Claims I3D '99: Proceedings of the 1999 symposium on Interactive 3D graphicsApril 1999Pages 89–95https://doi.org/10.1145/300523.300533Published:26 April 1999Publication History 129citation1,108DownloadsMetricsTotal Citations129Total Downloads1,108Last 12 Months63Last 6 weeks7 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins (2010)

RNA transcripts are subjected to post-transcriptional gene regulation by interacting with hundreds of RNA-binding proteins (RBPs) and microRNA-containing ribonucleoprotein complexes (miRNPs) that are often expressed in a cell-type dependently. To understand how the interplay of these RNA-binding factors affects the regulation of individual transcripts, high resolution maps of in vivo protein-RNA interactions are necessary. A combination of genetic, biochemical and computational approaches are typically applied to identify RNA-RBP or RNA-RNP interactions. Microarray profiling of RNAs associated with immunopurified RBPs (RIP-Chip) defines targets at a transcriptome level, but its application is limited to the characterization of kinetically stable interactions and only in rare cases allows to identify the RBP recognition element (RRE) within the long target RNA. More direct RBP target site information is obtained by combining in vivo UV crosslinking with immunoprecipitation followed by the isolation of crosslinked RNA segments and cDNA sequencing (CLIP). CLIP was used to identify targets of a number of RBPs. However, CLIP is limited by the low efficiency of UV 254 nm RNA-protein crosslinking, and the location of the crosslink is not readily identifiable within the sequenced crosslinked fragments, making it difficult to separate UV-crosslinked target RNA segments from background non-crosslinked RNA fragments also present in the sample. We developed a powerful cell-based crosslinking approach to determine at high resolution and transcriptome-wide the binding sites of cellular RBPs and miRNPs that we term PAR-CliP (Photoactivatable-Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation) (see Fig. 1A for an outline of the method). The method relies on the incorporation of photoreactive ribonucleoside analogs, such as 4-thiouridine (4-SU) and 6-thioguanosine (6-SG) into nascent RNA transcripts by living cells. Irradiation of the cells by UV light of 365 nm induces efficient crosslinking of photoreactive nucleoside-labeled cellular RNAs to interacting RBPs. Immunoprecipitation of the RBP of interest is followed by isolation of the crosslinked and coimmunoprecipitated RNA. The isolated RNA is converted into a cDNA library and deep sequenced using Solexa technology. One characteristic feature of cDNA libraries prepared by PAR-CliP is that the precise position of crosslinking can be identified by mutations residing in the sequenced cDNA. When using 4-SU, crosslinked sequences thymidine to cytidine transition, whereas using 6-SG results in guanosine to adenosine mutations. The presence of the mutations in crosslinked sequences makes it possible to separate them from the background of sequences derived from abundant cellular RNAs. Application of the method to a number of diverse RNA binding proteins was reported in Hafner et al.
Surface micromachined and integrated capacitive sensors for microfluidic applications (2004)

We have demonstrated an entire series of capacitive sensors using a multi-layer parylene/photoresist surface micromachining technology. The developed sensors are designed for total integration into parylene-based microfluidic systems for real-time system monitoring. Sensors have been demonstrated for the following applications: in-line pressure sensing (range: 0-35 kPa, resolution: 0.03 kPa); liquid front position and/or volumetric measurements (range: 0->50 pL, resolution: <5 pL); and dielectric measurements, which can be used to deduce fluid properties such as liquid composition. The reported sensor technology demonstrates versatility, high sensitivity, small footprints, and easy integration.
Nijmegen breakage syndrome in a Dutch patient not resulting from a defect in <i>NBS1</i> (2001)

Contains fulltext : 144682.pdf (Publisher’s version ) (Open Access)
Stable Mesoscopic Dye-Sensitized Solar Cells Based on Tetracyanoborate Ionic Liquid Electrolyte (2006)

A stable dye-sensitized solar cell has been obtained based on a new binary ionic liquid electrolyte system containing 1-propyl-3-methylimidazolium iodide and 1-ethyl-3-methylimidazolium tetracyanoborate.
Flexible Electrochromic Display (2013)

We have developed Electrochromic materials that show various colors based on bi-pyridine compounds. These are adsorbed on the surface of nanostructured Ttitanium dioxide layer as a Gratzel cell structure by low-temperature solution-process. By utilizing flexible TFT, we have demonstrated full-color flexible electronic papers.
Toward Separation and Purification of Olefins Using Dithiolene Complexes: An Electrochemical Approach (2001)

The complex Ni[S2C2(CF3)2]2 reacts with light olefins, including ethylene and propylene, selectively and reversibly. The reaction is not poisoned by hydrogen gas, carbon monoxide, acetylene, or hydrogen sulfide, which are commonly present in olefin streams, presumably because olefin binding occurs through the sulfur ligand rather than the metal center. The reversible reaction of olefins with Ni[S2C2(CN)2]2n (n = 0, -1, -2) can be controlled electrochemically, where the oxidation state-dependent binding and release of olefins are fast on the electrochemical time scale. The observed tolerance to poisons and controllable electrochemical reactivity present an alternative approach to the separation of olefins from complex streams.
Lysis of Prostate Carcinoma Cells by Trifunctional Bispecific Antibodies (αEpCAM × αCD3) (2001)

Bispecific monoclonal antibodies (bsAbs) are a promising immunotherapeutic option for treatment of cancer, especially in situations of minimal residual disease. The combination of an anti-CD3 and anti-tumor-associated antigen antibody redirects cytotoxic T-lymphocytes towards malignant cells. Using a trifunctional bispecific antibody against Ep-CAM × CD3, that additionally activates FcγR + accessory cells via its Fc region, we investigated the interaction between three EpCAM + prostate carcinoma cell lines and peripheral blood mononuclear cells (PBMCs) of healthy donors and patients with prostate carcinoma (PC). Visualization was performed by double immunocytochemical methods and computerized sequential video microscopy. Tumor cells and PBMCs supplemented with αEpCAM × αCD3 in 16-well chamber slides resulted in lysis of tumor cells within 1–3 days without any differences between patient and healthy donor PBMCs. The characteristic necrotic way of tumor cell killing (rounding, swelling, disrupting) could be observed in computerized sequences of video frames. Simultaneously, we could not reveal any form of apoptotic signal using three different apoptotic markers (TUNEL, M30 cyto death, anti-active caspase 3). Within the first 48 hr we observed typical PBMC cluster formation with increasing cell proliferation. PBMCs surrounding the tumor cells were not dominated by CD4 + , CD8 + , or CD14 + cells. Lymphocytes with pore-forming perforin proteins concentrated towards the tumor target cells. Our combination of double immunocytochemical and computerized video microscopic techniques may serve as an important improvement of validity of cell–cell interaction experiments using in vitro models.
Reliability/Design Assessment by Internal-Node Timing-Margin Analysis using Laser Photocurrent-Injection (1984)

A new device analysis technique is discussed which may prove useful in characterizing critical timing paths and analyzing timing related failure modes in high clock rate VLSI circuits. A focussed laser is used to inject localized photocurrent at the drain of a single transistor as the device is tested. The resulting effects on circuit performance have been studied and it is shown that individual circuit path propagation delays can be increased in this manner. To assign a relative value to a measurement, the operating frequency, power supply voltage or injected photocurrent level can be varied with the others fixed until the device just fails. Models, circuit simulations and experimental results obtained using this technique on a simple gate and results measured on CMOS microprocessors are given in this paper. Possible applications of the technique beyond the analysis of frequency sensitive failures include experimentally locating performance limiting timing paths in a circuit and assessing a circuit's sensitivity to process or failure mechanism induced drifts in device parameters which affect timing.
Novel Coupling Cancellation Method by Loading Planar Path for Wideband High-Isolation Wide-Scanning Millimeter-Wave Phased Array (2022)

A novel coupling-cancellation method by loading planar path is proposed and applied in the millimeter-wave phased array for wide-angle scanning. By employing the improved decoupling stubs with weak radiation first, an extra introduced wideband equal-magnitude and anti-phase path is generated to cancel the inherent coupling path. The principle of the proposed method is investigated by the equivalent circuits and the full-wave simulation. Based on this method, a high-isolation 1-D phased array using low-temperature-cofired ceramic (LTCC) process is designed and fabricated. The planar paths are placed symmetrically between the subarrays and its radiation polarization is coincident with the antenna elements, resulting in high isolation and enhanced boresight radiation null-fill of subarrays. The measured isolation is below 20 dB within the relative bandwidth of 16.7% and the maximum scanning angle is ±60°. Moreover, the proposed method can be expectedly extended to large-scale arrays.
Photoluminescence characterization of scintillators for phoswich detectors (2009)

The challenge for advanced PET instrumentation is performance optimization in terms of spatial resolution and sensitivity. The phoswich approach, which leads to a better approximation of the interaction point, provides Depth-Of-Interaction information that can significantly reduce parallax error and improve image resolution. In some cases, the scintillator phoswich layers can have not only different decay times but also different characteristics such as light yields, emission spectra, densities, effective atomic numbers, and indices of refraction. Depending on these properties, light interactions between crystals are possible, which may either limit or enhance phoswich functionality. In this paper, we report experiments on the luminescence characteristics of some promising scintillator materials for PET which could be used in a phoswich detector, such as LaBr <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> :Ce, LaCl <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> :Ce, LSO:Ce, GSO:Ce, LPS:Ce, LuYAP:Ce, with an emphasis on investigating excitation/emission spectra, rise times and decay times. For each combination of front and back layer crystals, radioluminescence and photoluminescence spectra were obtained. A Bollinger-Thomas set-up was used to measure luminescence rise and luminescence decay characteristics. Light interactions between scintillator crystals in phoswich detectors were studied and the possibility of measuring convolved light as a result of absorption and re-emission by another crystal layer was experimentally evaluated.
Overlock-Stitched Stretch Sensors: Characterization and Effect of Fabric Property (2013)

The comfort of wearable sensors is often limited by the materials and construction methods of sensors. Here, we present a textile-based stretch sensor that is formed using a common industrial sewing machine, in an overlock formation. Swapping a single thread in the stitch for a conductive thread renders the stitch responsive to stretch through opening and closing short circuits in the looped structure of the exposed conductor. However, the sensor response is influenced by the mechanical properties of the textile to which it is stitched. We explore the influence of fiber content (with an emphasis on elastomeric fiber content) on the baseline drift, response range, and hysteresis of the stitched sensor. Results show that the sensor provides a reliable and repeatable response, with a linear region that spans from the relaxed position to between 18 and 29% elongation. Average drift of about 0.5 Ohm and hysteresis of about 1 Ohm were measured. Effects of fiber content were observed, but do not show clear relationships to percentage of elastomer content.
Lightweight (AlGaAs)GaAs/CuInSe/sub 2/ tandem junction solar cells for space applications (1989)

Mechanically stacked tandem cells consisting of GaAs thin-film upper cells and CuInSe/sub 2/ thin-film lower cells have been developed to meet the increasing power needs projected for future spacecraft. The authors report the fabrication of the first highly efficient lightweight GaAs/CuInSe/sub 2/ tandem cell on a 2-mil thick substrate, update recent performance improvements in thin-film GaAs/CuInSe/sub 2/ tandem cells, and discuss their application to space power systems. The efficiency of 4-cm/sup 2/ cells has improved to 21.6% AM0, the highest ever reported for a thin-film photovoltaic cell. Lightweight 4-cm/sup 2/ tandem cells have been successfully fabricated with efficiencies as high as 20.8%. These cells weighed about 180 mg without optimized substrate trimming. Radiation and operating temperature effects on GaAs/CuInSe/sub 2/ tandem cells are also discussed, and an interconnect scheme to form a voltage-matched string is described.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Quantifying the effects of hydration on corneal stiffness with optical coherence elastography (2018)

Several methods have been proposed to assess changes in corneal biomechanical properties due to various factors, such as degenerative diseases, intraocular pressure, and therapeutic interventions (e.g. corneal collagen crosslinking). However, the effect of the corneal tissue hydration state on corneal stiffness is not well understood. In this work, we induce low amplitude (< 10 μm) elastic waves with a focused micro air-pulse in fresh in situ rabbit corneas (n = 10) in the whole eye-globe configuration at an artificially controlled intraocular pressure. The waves were then detected with a phase-stabilized swept source optical coherence elastography system. Baseline measurements were taken every 20 minutes for an hour while the corneas were hydrated with 1X PBS. After the measurement at 60 minutes, a 20% dextran solution was topically instilled to dehydrate the corneas. The measurements were repeated every 20 minutes again for an hour. The results showed that the elastic wave velocity decreased as the corneal thickness decreased. Finite element modeling (FEM) was performed using the corneal geometry and elastic wave propagation speed to assess the stiffness of the samples. The results show that the stiffness increased from ~430 kPa during hydration with PBS to ~500 kPa after dehydration with dextran, demonstrating that corneal hydration state, apart from geometry and intraocular pressure, can change the stiffness of the cornea.
A magnetic-bead based microfludic system for rapid detection of immunoglobulins (2009)

The current study reports an integrated microfluidic system for rapid serological analysis of antibodies associated with the infection of dengue virus by utilizing virus-holding magnetic beads. This microfluidic system integrates micropumps, a four-membrane-type micromixer, and on-chip microcoils array to perform the rapid detection of immunoglobulin G (IgG) and immunoglobulin M (IgM) simultaneously. Using virus-holding magnetic beads, IgG and IgM in a clinical serum samples can be captured, followed by purification of these beads utilizing the on-chip microcoils array. Finally, the optical signals of sandwich-like magnetic complexes can be detected by an optical detection module. The whole reaction time is measured to be less than 30 minutes. More importantly, the detection limit has been improved to 21 pg.
Improved Fourier transform technique to determine secondorder optical nonlinearity profiles (2003)

We report a novel Maker-fringe measurement technique to determine uniquely the nonlinearity profile of thin nonlinear materials such as poled silica, which offers a greater accuracy as well as a much simpler and faster data processing algorithm.
Supplementary Figure 4 from Macrophage PI3Kγ Drives Pancreatic Ductal Adenocarcinoma Progression (2023)

<p>Effect of PI3Kγ inhibitors on PDAC growth and survival.</p>
A novel autoantibody recognizing 200‐kd and 100‐kd proteins is associated with an immune‐mediated necrotizing myopathy (2010)

An anti-200/100-kd specificity defines a subgroup of patients with necrotizing myopathy who previously were considered to be autoantibody negative. We propose that these patients have an immune-mediated myopathy that is frequently associated with prior statin use and should be treated with immunosuppressive therapy.
Electrical conductivity of novel Langmuir–Blodgett films containing ethyl β-apo-8′-carotenoate, an amphiphilic carotenoid (1989)

The first preparation of stable monomolecular layers and Langmuir–Blodgett films containing the title compound is reported; electrical conductivities parallel and perpendicular to the layer plane have been determined prior and subsequent to iodine vapour treatment of the films.
Novel Cell Culture-Adapted Genotype 2a Hepatitis C Virus Infectious Clone (2012)

Although the recently developed infectious hepatitis C virus system that uses the JFH-1 clone enables the study of whole HCV viral life cycles, limited particular HCV strains have been available with the system. In this study, we isolated another genotype 2a HCV cDNA, the JFH-2 strain, from a patient with fulminant hepatitis. JFH-2 subgenomic replicons were constructed. HuH-7 cells transfected with in vitro transcribed replicon RNAs were cultured with G418, and selected colonies were isolated and expanded. From sequencing analysis of the replicon genome, several mutations were found. Some of the mutations enhanced JFH-2 replication; the 2217AS mutation in the NS5A interferon sensitivity-determining region exhibited the strongest adaptive effect. Interestingly, a full-length chimeric or wild-type JFH-2 genome with the adaptive mutation could replicate in Huh-7.5.1 cells and produce infectious virus after extensive passages of the virus genome-replicating cells. Virus infection efficiency was sufficient for autonomous virus propagation in cultured cells. Additional mutations were identified in the infectious virus genome. Interestingly, full-length viral RNA synthesized from the cDNA clone with these adaptive mutations was infectious for cultured cells. This approach may be applicable for the establishment of new infectious HCV clones.
Detection of spam hosts and spam bots using network flow traffic modeling (2010)

e-mail: {wehrlich,karasaridis,dliu,dhoeflin} at att dot com Abstract—In this paper, we present an approach for detecting e-mail spam originating hosts, spam bots and their respective controllers based on network flow data and DNS metadata. Our approach consists of first establishing SMTP traffic models of legitimate vs. spammer SMTP clients and then classifying unknown SMTP clients with respect to their current SMTP traffic distance from these models. An entropy-based traffic component extraction algorithm is then applied to traffic flows of hosts identified as e-mail spammers to determine whether their traffic profiles indicate that they are engaged in other exploits. Spam hosts that are determined to be compromised are processed further to determine their command-and-control using a two-stage approach that involves the calculation of several flow-based metrics, such as distance to common control traffic models, periodicity, and recurrent behavior. DNS passive replication metadata are analyzed to provide additional evidence of abnormal use of DNS to access suspected controllers. We illustrate our approach with examples of detected controllers in large HTTP(S) botnets such as Cutwail, Ozdok and Zeus, using flow data collected from our backbone network. I.
Genetic variation in the fibrinogen gamma gene increases the risk for deep venous thrombosis by reducing plasma fibrinogen γ′ levels (2005)

We investigated the association between haplotypes of fibrinogen alpha (FGA), beta (FGB), and gamma (FGG), total fibrinogen levels, fibrinogen gamma' (gammaA/gamma' plus gamma'/gamma') levels, and risk for deep venous thrombosis. In a population-based case-control study, the Leiden Thrombophilia Study, we typed 15 haplotype-tagging single nucleotide polymorphisms (htSNPs) in this gene cluster. None of these haplotypes was associated with total fibrinogen levels. In each gene, one haplotype increased the thrombosis risk approximately 2-fold. After adjustment for linkage disequilibrium between the genes, only FGG-H2 homozygosity remained associated with risk (odds ratio [OR], 2.4; 95% confidence interval [95% CI], 1.5-3.9). FGG-H2 was also associated with reduced fibrinogen gamma' levels and reduced ratios of fibrinogen gamma' to total fibrinogen. Multivariate analysis showed that reduced fibrinogen gamma' levels and elevated total fibrinogen levels were both associated with an increased risk for thrombosis, even after adjustment for FGG-H2. A reduced fibrinogen gamma' to total fibrinogen ratio (less than 0.69) also increased the risk (OR, 2.4; 95% CI, 1.7-3.5). We propose that FGG-H2 influences thrombosis risk through htSNP 10034C/T [rs2066865] by strengthening the consensus of a CstF site and thus favoring the formation of gammaA chain above that of gamma' chain. Fibrinogen gamma' contains a unique high-affinity, nonsubstrate binding site for thrombin, which seems critical for the expression of the antithrombin activity that develops during fibrin formation (antithrombin 1).