gebhart/patrepeval huggingface dataset:
Regulation of T cell afferent lymphatic migration by targeting LTβR-mediated non-classical NFκB signaling (2018)

Lymphotoxin-beta receptor (LTβR) signaling in lymphatic endothelial cells (LEC) regulates leukocyte afferent lymphatic transendothelial migration (TEM). The function of individual signaling pathways for different leukocyte subsets is currently unknown. Here, we show that LTβR signals predominantly via the constitutive and ligand-driven non-classical NIK pathway. Targeting LTβR-NIK by an LTβR-derived decoy peptide (nciLT) suppresses the production of chemokines CCL21 and CXCL12, and enhances the expression of classical NFκB-driven VCAM-1 and integrin β4 to retain T cells on LEC and precludes T cell and dendritic cell TEM. nciLT inhibits contact hypersensitivity (CHS) at both the sensitization and elicitation stages, likely by inhibiting leukocyte migration. By contrast, targeting LTβR-classical NFκB signaling during the elicitation and resolution stages attenuates CHS, possibly by promoting leukocyte egress. These findings demonstrate the importance of LTβR signaling in leukocyte migration and LEC and lymphatic vessel function, and show that antagonist peptides may serve as lead compounds for therapeutic applications.
A Double-Sided <italic>LCLC</italic>-Compensated Capacitive Power Transfer System for Electric Vehicle Charging (2015)

A double-sided LCLC-compensated capacitive power transfer (CPT) system is proposed for the electric vehicle charging application. Two pairs of metal plates are utilized to form two coupling capacitors to transfer power wirelessly. The LCLC-compensated structure can dramatically reduce the voltage stress on the coupling capacitors and maintain unity power factor at both the input and output. A 2.4-kW CPT system is designed with four 610-mm × 610-mm copper plates and an air gap distance of 150 mm. The experimental prototype reaches a dc-dc efficiency of 90.8% at 2.4-kW output power. At 300-mm misalignment case, the output power drops to 2.1 kW with 90.7% efficiency. With a 300-mm air gap distance, the output power drops to 1.6 kW with 89.1% efficiency.
Synthesis and structure-activity relationship of 1-aryl-6,8-difluoroquinolone antibacterial agents (1987)

A series of new arylfluoroquinolones has been prepared. These derivatives are characterized by having fluorine atoms at the 6- and 8-positions, substituted amino groups at the 7-position, and substituted phenyl groups at the 1-position. The in vitro antibacterial potency is greatest when the 1-substituent is 2,4-difluorophenyl and the 7-substituent is a 3-amino-1-pyrrolidinyl group. 1-(4-Fluorophenyl)-6,8-difluoro-7-piperazin-1-yl-1,4-dihydro-4-oxo quinoline-3- carboxylic acid (22) was found to possess excellent in vitro potency and in vivo efficacy.
Steady‐state sequence synthesis and its application to efficient fat‐suppressed imaging (2003)

Abstract A new synthesis algorithm, based on the Shinnar‐Le Roux (SLR) transform, can be used to generate fully refocused steady‐state pulse sequences with arbitrary magnetization profiles as a function of off‐resonant precession. This is accomplished by appropriate periodic oscillation of the RF excitation magnitude and phase from echo to echo. The technique is applied to the design of refocused steady‐state free precession (SSFP) sequences with flat profiles, providing the opportunity for banding‐artifact‐free imaging with steady‐state contrast. The algorithm is also used to generate refocused‐SSFP sequences with an arbitrarily broad region of attenuated signal. These sequences are implemented and applied to the problem of steady‐state fat suppression. Preliminary results show signal levels that agree well with theory, and a broad region of suppressed signal at each echo. Total imaging time is kept identical to that of a standard refocused‐SSFP experiment through echo equalization and interleaving. 3D images from the leg of a normal volunteer acquired in 44 s demonstrate the applicability of the technique to fat‐suppressed imaging. Magn Reson Med 50:550–559, 2003. © 2003 Wiley‐Liss, Inc.
Confidence-Weighted Learning of Factored Discriminative Language Models (2011)

Language models based on word surface forms only are unable to benefit from available linguistic knowledge, and tend to suffer from poor estimates for rare features. We propose an approach to overcome these two limitations. We use factored features that can flexibly capture linguistic regularities, and we adopt confidence-weighted learning, a form of discriminative online learning that can better take advantage of a heavy tail of rare features. Finally, we extend the confidence-weighted learning to deal with label noise in training data, a common case with discriminative language modeling.
Electrode-material dependent switching in TaO<sub><i>x</i></sub>memristors (2014)

We have studied the effect of top electrode materials on the switching behavior of TaOx based memristors with an identical switching oxide layer and bottom electrode stack. We found that the virgin resistance, electroforming and switching performance depend heavily on the chemical property of the top electrode materials. In addition, the electrical properties of metal oxides formed with the top electrodes also contribute to the overall memristor performance, including the nonlinearity of the current–voltage relationship. These results provide insights into understanding of memristor behavior as well as approaches for device property engineering.
Pre-T cell receptor for improved expansion of TCR alpha disrupted T cells (2013)

Meeting abstracts Recent data have emerged from adoptive T-cell therapies where exogenous expression of a chimeric antigen receptor (CAR) has been shown to confer cancer recognition on autologous T cells. However, the ability to apply this technology in an allogeneic setting would permit the
Privacy-Preserving Social Tie Discovery Based on Cloaked Human Trajectories (2016)

The discovery of peoples' social connections is becoming a flourishing research topic, considering the rich social information inferable from human trajectories. Existing social tie detection methods often require mobile users to upload their accurate locations, causing serious privacy concerns. On the other hand, cloaking methods allow users to upload their obscured locations instead and can efficiently protect their location privacy. However, no existing social tie detection method can generate social relationships among users when only obscured trajectories are provided. To tackle the aforementioned problem, this paper proposes a novel semantic-tree-based algorithm. Specifically, we model the obscured regions from the cloaking algorithm as a semantic region tree and assign weight values for regions based on their popularity, further indicating the similarity between users based on their temporal and spatial relations. We evaluate our proposed approach using a real trajectory data set and show that our algorithm can identify social ties successfully with 20% higher accuracy than the existing approaches.
Development of a novel equine influenza virus live-attenuated vaccine (2018)

H3N8 equine influenza virus (EIV) is an important and significant respiratory pathogen of horses. EIV is enzootic in Europe and North America, mainly due to the suboptimal efficacy of current vaccines. We describe, for the first time, the generation of a temperature sensitive (ts) H3N8 EIV live-attenuated influenza vaccine (LAIV) using reverse-genetics approaches. Our EIV LAIV was attenuated (att) in vivo and able to induce, upon a single intranasal administration, protection against H3N8 EIV wild-type (WT) challenge in both a mouse model and the natural host, the horse. Notably, since our EIV LAIV was generated using reverse genetics, the vaccine can be easily updated against drifting or emerging strains of EIV using the safety backbone of our EIV LAIV as master donor virus (MDV). These results demonstrate the feasibility of implementing a novel EIV LAIV approach for the prevention and control of currently circulating H3N8 EIVs in horse populations.
Quadband Petal Reflector Antenna for Ground Communications: Combining four frequency bands into one innovative and reliable design (2023)

Future protected communications require soldiers on the ground to carry several antennas integrated with their manpacks to provide secure and reliable communications at a number of frequency bands. This article describes a novel quadband petal reflector antenna (QPRA) that combines four frequency bands into one using a quadruplexer. A dual-reflector antenna utilizing an axially displaced ellipsoid subreflector being fed with a quad-ridged horn provides communications at Ku-band transmit (Ku-Tx), Ku-band receive (Ku-Rx), K-band receive (K-Rx), and Ka-band transmit (Ka-Tx) frequencies simultaneously. The main reflector is made of six identical petals that can be quickly assembled and disassembled in the battlefield. The antenna is mounted on a lightweight gimbal to provide beam scanning over a hemispherical coverage. The antenna is mounted on a tripod structure for field operations and uses commercial off the shelf (COTS) components and 3D manufactured parts to minimize cost and mass. The novelty of the antenna presented in this article includes a wide bandwidth capability of 73%, the use of a petal reflector, a 3D manufactured quad-ridged horn, a high-performance quadruplexer, and the use of COTS gimbals for beam scanning. Antenna design, analyses, hardware, and test results are presented in this article. The measured results of the antenna agree well with the computed results validating the QPRA design.
Y5U multilayer ceramic capacitors with high-specific capacitance and low-equivalent series resistance (1991)

A dielectric powder material, for Y5U multilayer ceramic capacitors was developed in the Pb(Mg(1/3)Nb(2/3))O (3)-Pb(Ni(1/3)Nb(2/3))O(3)-PbTiO (3) ternary system by using an alkoxide process. Multilayer ceramic chip capacitors (10 muF) with high specific capacitances were fabricated using this powder. A Ag80%-Pd20% alloy was used for the internal electrodes. The alkoxide-derived capacitor had an extremely high specific capacitance of about 500 muF/cm(3) and a small temperature dependence meeting Y5U specifications of the EIA standard. The equivalent series resistance was approximately 20 mOmega at 500 kHz. The breakdown voltages of the capacitor were 300 V or higher. In accelerated load life tests and load humidity tests, no degradation of insulation resistance was found during 1000 h of testing.
Fluorescent indicators for cytosolic sodium (1989)

Fluorescent indicators sensitive to cytosolic concentrations of free Na+ have been synthesized and characterized. They consist of a crown ether, 1,7-diaza-4,10,13-trioxacyclopentadecane, linked via its nitrogens to fluorophores bearing additional liganding centers. In the currently preferred dye, SBFI (short for sodium-binding benzofuran isophthalate), the fluorophores are benzofurans linked to isophthalate groups. Selectivities for Na+ over K+ of about 20 are observed, resulting in effective dissociation constants for Na+ of about 20 mM against a background of 120 mM K+. Increasing [Na+] increases the ratio of excitation efficiency at 330-345 nm to that at 370-390 nm with emission collected at 450-550 nm, so that ratio fluorometry and imaging can be performed with the same wavelengths as used with the well known Ca2+ indicator fura-2. If the macrocyclic ring is increased in size to a 1,10-diaza-4,7,13,16-tetraoxacyclooctadecane, the chelators become selective for K+ over Na+.
Factors affecting white cell content in platelet concentrates (1985)

In this study, we investigated the factors affecting white cell content in platelet concentrates. White cell yields can be reduced 50 percent by stopping platelet-rich plasma expression when the interface is 1 cm from the top of the blood bag as compared to stopping expression when the interface reaches the top of the bag. Further reductions can be achieved by careful handling during transfer of units from the centrifuge cups to expressors (after the first spin) and by carefully balancing units against each other to ensure proper rotor balance during the first spin. Following these suggestions, blood banks should be able to produce platelet concentrates with white cell yields between 2 and 6 X 10(7) and with platelet yields between 7.5 and 8 X 10(10). Transfusion of this product may reduce febrile reactions and lower the incidence of alloimmunizations.
Selectively retrofitting monitoring in distributed systems (2009)

Current distributed systems carry legacy subsystems lacking sufficient instrumentation for monitoring the end-to-end business transactions supported by these systems. In the absence of instrumentation, only probabilistic monitoring is possible by using time-stamped log-records. Retro fitting these systems with expensive monitoring instrumentation provides high-granularity, precise tracking of transactions. Given a limited budget, local instrumentation strategies which maximize the effectiveness of monitoring transactions throughout the system are proposed. The operation of the end-to-end system is modeled by a queuing network;each queue represents a subsystem which produces time-stamped log-records as transactions pass through it. Two simple heuristics for instrumentation are proposed which become optimal under certain conditions. One heuristic selects states in the transition diagram for local instrumentation in the decreasing order of the load factors of their queues. Sufficient onditions for this load-factor heuristic to be optimal are proven using the notion of stochastic order. The other heuristic selects states in the transition diagram based on the approximated tracking accuracy of probabilistic monitoring at each state, which is shown to be tight at low arrival rates.
Fluorescence Spectroscopic Imaging to Detect Changes in Collagen and Elastin Following Laser Tissue Welding (2000)

Optical spectroscopic imaging offers a new noninvasive detecting method for microscopic evaluation of laser tissue welding.
Distributed receding horizon control of cost coupled systems (2007)

This paper considers the problem of distributed control of dynamically decoupled systems that are subject to decoupled constraints, while their states are coupled non- separably in the cost function of an optimal control problem. A distributed receding horizon control (RHC) algorithm is presented, in which each subsystem (agent) computes its own control locally. The implementation presumes synchronous parallel updates. Coupled agents, referred to as neighbors, coordinate by the exchange of an assumed state trajectory prior to each update. The algorithm is an improvement over the previous algorithm developed by the author, in that stability is guaranteed without adding any additional constraints to each local optimal control problem. Instead, a move suppression term is added into each local cost function, which penalizes the deviation of the computed state trajectory from the assumed state trajectory. Closed-loop stability follows if the weight on the move suppression term is larger than a parameter that bounds the amount of coupling in the cost function between neighbors.
A pH‐Neutral, Metal‐Free Aqueous Organic Redox Flow Battery Employing an Ammonium Anthraquinone Anolyte (2019)

Redox-active anthraquinone molecules represent promising anolyte materials in aqueous organic redox flow batteries (AORFBs). However, the chemical stability issue and corrosion nature of anthraquinone-based anolytes in reported acidic and alkaline AORFBs constitute a roadblock for their practical applications in energy storage. A feasible strategy to overcome these issues is migrating to pH-neutral conditions and employing soluble AQDS salts. Herein, we report the 9,10-anthraquinone-2,7-disulfonic diammonium salt AQDS(NH<sub>4</sub> )<sub>2</sub> , as an anolyte material for pH-neutral AORFBs with solubility of 1.9 m in water, which is more than 3 times that of the corresponding sodium salt. Paired with an NH<sub>4</sub> I catholyte, the resulting pH-neutral AORFB with an energy density of 12.5 Wh L<sup>-1</sup> displayed outstanding cycling stability over 300 cycles. Even at the pH-neutral condition, the AQDS(NH<sub>4</sub> )<sub>2</sub> /NH<sub>4</sub> I AORFB delivered an impressive energy efficiency of 70.6 % at 60 mA cm<sup>-2</sup> and a high power density of 91.5 mW cm<sup>-2</sup> at 100 % SOC. The present AQDS(NH<sub>4</sub> )<sub>2</sub> flow battery chemistry opens a new avenue to apply anthraquinone molecules in developing low-cost and benign pH-neutral flow batteries for scalable energy storage.
Generation of Functional Lymphoid (Natural Killer) Cells From Human ESC-Derived Hemangioblasts. (2009)

Abstract Abstract 1502 Poster Board I-525 Studies with human and mouse embryonic stem cells (ESCs) have shown that a common precursor to both vascular (endothelial and smooth muscle cells) and hematopoietic cell lineages called the hemangioblast can be produced from ESC-derived embryoid bodies in culture. We have developed a simple strategy to efficiently and reproducibly generate hemangioblasts from multiple hESC lines under serum- and stromal-free conditions, which will be important for their productive use in regenerative medicine. Previous work has shown that hESC-derived hemangioblasts can effectively differentiate into erythroid and myeloid lineages, but their ability to produce lymphoid lineage cells, including those with immunotherapeutic potential, is relatively unknown. Natural killer (NK) cells, which are part of the innate immune system, provide rapid, non-specific responses against viral infection and are involved in tumor cell detection and elimination. Interplay between various activating and inhibitory signals control the three main functions of NK cells, which are cytokine release, natural cytotoxicity, and antibody-dependent cellular cytotoxicity. Using hemangioblasts generated from both H7 and HuES-3 hESC lines, we have been able to produce mature CD56low/−CD16+ NK cells and found that their production does not require the use of stromal feeder layers. The differentiation procedure involves an initial 4 day culture to generate embryoid bodies, followed by a 12-14 day culture in methylcellulose supplemented with a set of cytokines and growth factors for the production and expansion of a hemangioblastic population. An additional 14-17 days in liquid culture plus human serum and a cocktail of cytokines allows for the differentiation of NK cells as assessed by flow cytometry. A non-radioactive cytotoxicity assay similar to the 51Cr release assay shows that these hemangioblast-derived NK cells harbor natural cytotoxicity function as they are able to effectively induce apoptosis in target K562 erythroblastic leukemia cells after a standard 4 hr co-culture. Using hemangioblasts as an intermediary cell source may enhance the capability and/or efficiency of hESCs to differentiate in vitro and importantly, allow for the development of feeder-free systems for the production of cells with immunotherapeutic potential. Disclosures: Kimbrel: Stem Cell & Regenerative Medicine International: Employment. Lu: Stem Cell & Regenerative Medicine International: Employment. Lanza: Stem Cell & Regenerative Medicine International/Advanced Cell Technology: Employment.
336. Developing a Protein Signature of Early Latent Tuberculosis Infection. (2022)

Abstract Background To curb the tuberculosis (TB) epidemic, new cases must be prevented. Preventive therapy is an effective intervention but treating all individuals with latent TB infection (LTBI) based on current diagnostics, tuberculin skin test (TST) and interferon gamma release assay (IGRA), is not feasible. Using proteomic analysis we are developing a peripheral blood marker of LTBI infection that would identify people at high risk for TB progression. Methods Plasma was collected in an ongoing TB household contact study in Kampala, Uganda in which TST-/IGRA- are enrolled and followed for IGRA/TST conversion. Longitudinal sample sets (0,6,12 months) from 81 participants including 24 TST/IGRA converters, 29 resisters (remained TST/IGRA negative), and 28 baseline positives (LTBI) underwent proteomic and multiplex cytokine analysis. Proteomic analysis was performed by multiple reaction monitoring mass spectrometry (MRM-MS) using an MRM-MS assay containing 163 host proteins represented by 392 unique peptides, all of which we have described to significantly change during Mtb infection in our previous study of household contact converters. Results Differential expression of proteins measured by MRM-MS and cytokine assays were assessed. The largest number of significant changes were measured in the LTBI vs Converter comparison. The vast majority of the changes were elevated expression in the converter group compared to the latent group, suggesting that all the biologies affected were induced in the converters (Figure 1). We identified protein panels that were able to predict conversion as in our earlier study. However, the protein panels defined in our earlier study that could distinguish a future converter from a future resister with high fidelity did not have a similar performance in this study. Figure 1Differential expression analysis of latent TB, LTBI vs. converter. Significant changes by both p-value (set at 0.05) and q-value (a correction for multiple comparisons, set at 0.10). Conclusion Peripheral blood protein changes are associated with conversion to LTBI. In this study we found that the protein panels predictive of conversion were different from our previous study. While there are many possible reasons, an important difference between the two studies was the definition of conversion: TST in the older study and QuantiFERON in the current study. We are continuing to investigate this difference and developing time-sensitive protein panels to capture recent conversion. Disclosures Eustache Paramithiotis, PhD, CellCarta Biosciences: Employee.
Upconversion Fiber Lasers (1993)

Over the past two years, the uniform, high-intensity pumping advantages of the single-mode fiber geometry have been realized in demonstrating several new visible upconversion laser transitions. To date 11 different CW, room-temperature visible upconversion laser/pumping combinations have been demonstrated with visible laser powers of up to 200 mW. Perhaps the most dramatic demonstration of an upconversion laser to date was the CW, room-temperature operation of a Pr 3+ -doped ZBLAN fiber at 5 visible wavelengths 1 . An additional advantage of the fiber geometry for upconversion lasers is that the fiber laser is the only laser geometry where the pump spot size and resonator length are decoupled. This extra degree of freedom is extremely important in the upconversion process where the ground-state absorption (GSA) and one or more excited-state absorptions (ESA) must be balanced in order to efficiently populate the upper laser level. For example, the three-photon pumped Tm 3+ upconversion laser discussed in this paper would not be possible in a Tm 3+ -doped crystal due to the extremely weak GSA. The long resonator lengths possible in fiber lasers also allow the minimization of concentration quenching effects while distributing the absorption to facilitate heat removal.