gebhart/patrepeval huggingface dataset:
Analysis of Tissue Transglutaminase Function in the Migration of Swiss 3T3 Fibroblasts (2002)

Increasing evidence suggests that tissue transglutaminase (tTGase; type II) is externalized from cells, where it may play a key role in cell attachment and spreading and in the stabilization of the extracellular matrix (ECM) through protein cross-linking. However, the relationship between these different functions and the enzyme's mechanism of secretion is not fully understood. We have investigated the role of tTGase in cell migration using two stably transfected fibroblast cell lines in which expression of tTGase in its active and inactive (C277S mutant) states is inducible through the tetracycline-regulated system. Cells overexpressing both forms of tTGase showed increased cell attachment and decreased cell migration on fibronectin. Both forms of the enzyme could be detected on the cell surface, but only the clone overexpressing catalytically active tTGase deposited the enzyme into the ECM and cell growth medium. Cells overexpressing the inactive form of tTGase did not deposit the enzyme into the ECM or secrete it into the cell culture medium. Similar results were obtained when cells were transfected with tTGase mutated at Tyr(274) (Y274A), the proposed site for the cis,trans peptide bond, suggesting that tTGase activity and/or its tertiary conformation dependent on this bond may be essential for its externalization mechanism. These results indicate that tTGase regulates cell motility as a novel cell-surface adhesion protein rather than as a matrix-cross-linking enzyme. They also provide further important insights into the mechanism of externalization of the enzyme into the extracellular matrix.
Transport Properties of Solid Polymer Electrolytes Prepared from Oligomeric Fluorosulfonimide Lithium Salts Dissolved in High Molecular Weight Poly(ethylene oxide) (2006)

Transport properties such as ionic conductivity, lithium transference number, and apparent salt diffusion coefficient are reported for solid polymer electrolytes (SPEs) prepared using several oligomeric bis[(perfluoroalkyl)sulfonyl]imide (fluorosulfonimide) lithium salts dissolved in high molecular weight poly(ethylene oxide) (PEO). The salt series consists of polyanions in which two discrete fluorosulfonimide anions are linked together by [(perfluorobutylene)disulfonyl]imide linker chains. The restricted diffusion technique was used to measure the apparent salt diffusion coefficients in SPEs, and cationic transference numbers were determined using both potentiostatic polarization and electrochemical impedance spectroscopy methods. A general trend of diminished salt diffusion coefficient with increasing anion size was observed and is opposite to the trend observed in ionic conductivity. This unexpected finding is rationalized in terms of the cumulative effects of charge carrier concentration, anion mobility, ion pairing, host plasticization by the anions, and salt phase segregation on the conductivity.
Crystal structure of desheptapeptide(B24–B30)insulin at 1.6 Å resolution: Implications for receptor binding (1997)

The crystal structure of desheptapeptide (B24-B30) insulin (DHPI), a virtually inactive analog of insulin, was determined at 1.6 A resolution. In the refined structure model, DHPI retains three alpha-helices (A1-A8, A12-A18, and B9-B19) as its structural framework, while great conformational changes occur in the N and C termini of B-chain. The beta-turn, which lies in B20-B30 in insulin and insulin analogs with high potency, no longer exists in DHPI. Relative motion is observed among the three alpha-helices, each as a rigid functional group. In contrast, a region covering B5-B6 and A6-A11 exhibits a relatively stable conformation. We interpret our results as identifying: (i) the importance of beta-turn in determining the receptor-binding potency of insulin and (ii) a leading role of PheB24 in maintaining the beta-turn structure.
Evaluation of the Antibacterial Activity and Cytotoxicity of Extracts of Endophytic Bacteria Isolated from Solanum torvum Sw (Solanaceae) (2022)

The study aimed at evaluating the antibacterial and cytotoxic potential of endophytic bacteria extracts isolated from Solanum torvum Sw (Solanaceae), a plant traditionally used in the treatment of many pathologies such as diarrheal diseases. Isolation of endophytic bacteria was done on supplemented and non-supplemented nutrient media. The extracts of these endophytes were obtained after fermentation in Muller Hinton Broth (MHB). An antibacterial screening of the extracts was carried out on 04 bacterial strains (Staphylococcus aureus, Shigella flexneri, Escherichia coli and Salmonella typhimurium). The strains having demonstrated activity were retained for the determination of the minimum inhibitory concentrations (MIC). The cytotoxicity tests of the extracts were carried out on healthy macrophage cells (Raw 264.7) and healthy kidney cells (Vero). A total of 41 isolates of endophytic bacteria were isolated from S. torvum. The antibacterial screening made it possible to retain 08 extracts which demonstrated antibacterial activity on the S. aureus and S. flexneri strains with MICs ranging from 100 to 200 μg/ml. All extracts exhibited weak cytotoxic activity except for one extract. Extracts of endophytic bacteria isolated from S. torvum showed antibacterial activity and low cytotoxicity on the pathogenic strains tested. This study shows that the endophytic bacteria of S. torvum are a source of antibacterial compounds to be explored to enrich the therapeutic arsenal against bacterial infections.
Block Polymer Membranes Functionalized with Nanoconfined Polyelectrolyte Brushes Achieve Sub-Nanometer Selectivity (2017)

The well-defined nanostructure of membranes manufactured from self-assembled block polymers enables highly selective separations; however, recent efforts to push the pore size of block polymer-based membranes to the lower end of the size spectrum have only been moderately successful for a variety of reasons. For instance, the conformational changes of the stimuli-responsive functional groups lining the pore walls of some block polymer membranes result in varied pore sizes that limit their operational range. Here, we overcome this challenge through the directed design of the third moiety of an A-B-C triblock polymer. The use of this macromolecular design paradigm allows for the preparation of a 500 nm thick polyisoprene-<i>b</i>-polystyrene-<i>b</i>-poly(2-acrylamido-ethane-1,1-disfulonic acid) (PI-PS-PADSA) coating atop a hollow fiber membrane support. This nanoporous test bed, which exhibits an average pore radius of 1 nm, demonstrates an extremely high solute selectivity by fully gating solutes that have only an 8 Å size difference, a separation that is based solely on a sieving mechanism. Furthermore, the nanoscale structural characteristics of the solvated PADSA pore walls are elucidated by quantifying the rejection of neutral solutes and calculating the hydraulic permeability values in solutions of high ionic strength (1 mM ≤ <i>I</i> ≤ 3 M) and over a broad range of solution pH (1 ≤ pH ≤ 13). These key results provide a solid foundation for defining structure-property-performance relationships in the emerging area of nanoporous triblock polymer thin films. Moreover, the successful demonstration of the test bed separation device offers a tangible means by which to manufacture next-generation nanofiltration membranes that require a robust performance profile over a dynamic range of conditions.
A 0.6V 380&#x00B5;W &#x2212;14dBm LO-input 2.4GHz double-balanced current-reusing single-gate CMOS mixer with cyclic passive combiner (2009)

A mixer is one of the bottlenecks in achieving the low-voltage operation of a receiver. Most of the mixer topologies recently reported for low-voltage and low-power applications can be categorized into bulk-injection mixers or switching mixers. However, the bulk-injection mixer requires a large LO power with an accurate modeling of the back-gate effect [1]. Switching mixers also require a large LO power in the range of 0dBm for ideal switching operation [2–5]. In this work, a 0.6V mixer for Bluetooth specification with a target sensitivity of −90dBm [6], which requires a NF of 12dB and IIP3 of −7dBm, is described. None of the previously published designs [1–5] satisfies these specifications. A single-gate mixer [7], which was formerly used for mm-wave applications, is modified to fit the low-voltage and low-power 2.4GHz-band application.
Seismic Characterization of Upper Carboniferous Reservoirs by Means of Image Processing and Geostatistics&nbsp; :ABSTRACT (1997)

This DOI is not currently attached to any metadata records. DOIs can’t actually ever be deleted (they’re persistent), but sometimes our members create DOIs in error. We do have a process to approximate deletion which we follow only in rare cases where the DOI has been genuinely created in error, and most crucially, if the DOI has never been published anywhere online or in print and never otherwise distributed to or communicated with anyone (authors, readers, reviewers, etc.
Regulation of Glucose Metabolism in Livers and Kidneys of NOD Mice (1991)

Measurements were made of the levels of metabolic intermediates and activities of enzymes of the glycolytic route, pentose phosphate pathway, and polyol pathway in livers and kidneys of NOD mice. A 34% decrease in UDP-glucose, a 40% decrease in glucose-6-phosphate (G6P) and fructose-6-phosphate, and a 75% decrease in fructose-2,6-bisphosphate (F2,6P) were found in the livers of NOD mice. The fall in the level of F2,6P (the important regulator of glycolysis) is accompanied by a 20% reduction in the activity of phosphofructokinase. These changes are in agreement with previously reported liver depletion of glycogen and reduced synthesis of proteins and nucleic acids in the diabetic state. In the kidney, the increase in hexokinase activity is consistent with increased levels of G6P and glycogen content of kidney in diabetes. The decreased level of phosphoribosyl pyrophosphate was reported to be a regulator of kidney growth in the initial period of diabetes but can still be found in NOD mice 6 wk after development of hyperglycemia. The reported changes are similar to those seen in alloxan- or streptozocin-induced diabetic animals, but certain changes are more marked in NOD mice, especially those directed to increase nucleic acid and protein synthesis in the diabetic kidney.
Antimicrobial Resistance of Escherichia Coli Strains Isolated from Urine at Outpatient Population: a Single Laboratory Experience (2016)

The results of study showed that E. coli has the highest resistance to ampicillin and trimethoprim-sulfamethoxazole in outpatient population of Hrasno community.
INFLUENCE OF CELL WALL COMPOSITION ON THE RESISTANCE OF TWO <i>CHLORELLA</i> SPECIES (CHLOROPHYTA) TO DETERGENTS<sup>1</sup> (1996)

ABSTRACT The influence of dodecylbenzene sulfonate (DBS) and Triton X‐100 (TX‐100) was examined on two species of Chlorella exhibiting conspicuous differences in cell wall composition. Chlorella emersonii has both a classical polysaccharidic wall and a thin trilaminar outer wall (TLS) composed of nonhydrolyzable macromolecules. Chlorella vulgaris lacks a TLS. Photosynthetic capacity was measured following short exposures (1 h) of the algae at different physiological stages to high DBS and TX‐100 concentrations, up to 1 g·L −1 . Comparisons with untreated controls indicated that 1) the presence of a TLS in C. emersonii was associated with a very high resistance to the anionic (DBS) and nonionic (TX‐100) detergents at all growth stages, and net photosynthesis was not significantly affected in that species, 2) a high toxicity, particularly pronounced with TX‐100, was observed for actively growing cells of the TLS‐devoid species, C. vulgaris , and 3) aging exerted a protective influence, especially efficient against DBS, on the latter species. Additional observations, including fluorescence spectra and high‐performance liquid chromatography pigment analyses, were conducted following short exposures of actively growing cells. Fluorescence emission spectra revealed that the chlorophyll a‐protein complexes in thylakoid membranes were not substantially affected by DBS and TX‐100, even in the case of C. vulgaris. In sharp contrast, fluorescence excitation spectra on the latter species showed 1) that excitation transfer from antenna pigments to chlorophyll a in reaction centers was substantially altered with both detergents and 2) that the two detergents affected different parts of the photosynthetic system of the TLS‐devoid species. Analyses of C. vulgaris extracts indicated significant decreases in pigment content following exposure to DBS and, to a lesser extent, to TX‐100. Longer exposure experiments (1 day) were conducted with actively growing algae. The TLS‐containing species still showed a very high resistance and no important changes in photosynthetic capacity compared to cells exposed for 1 h. For the sensitive TLS‐devoid species, the detrimental influence of TX‐100, already very high after 1 h, was not increased. DBS toxicity was markedly increased and may reflect a lower uptake rate of DBS by C. vulgaris . Taken together, these observations confirm the important protective role of TLS against detergents. They also provide information on the factors controlling detergent toxicity in the sensitive, TLS‐devoid species and on the different modes of action of DBS and TX‐100 on its photosynthetic system. Such large differences in microalgal sensitivity to detergents, related to TLS occurrence, should have important consequences for the selection of suitable species in toxicity tests.
Relationele zorgrechtvaardigheid (2020)

Restorative justice as a relational justice of care As an approach to law and justice, restorative justice focuses squarely on addressing human needs; it acknowledges the equal dignity of all parties, irrespective of their role in the harmful act; it accepts responsibilities for addressing the disadvantages or inequities that crime has bequeathed to victims and that often have contributed to offending on the part of the perpetrator; and it derives its creative potential from the shared vulnerability and reciprocal exchange of concern that occurs through respectful face-to-face encounter. Therefore restorative justice constitutes a relational justice of care.
Influence of SLS processing parameters according to the new mathematical model on flexural properties (2016)

Purpose The purpose of this paper is the selection of production parameters in selective laser sintering – SLS for the production of quality products (e.g. good mechanical properties). The manufacturing procedure affects the properties, which is especially significant in additive procedures, as the products are not cheap and the procedure and material need to be optimally selected. Design/methodology/approach The paper presents an analysis of the fabrication parameters in selective laser sintering (SLS) that affect the flexural properties of the finished product. Findings The influence of a new parameter has been found, the overlay ratio, and using the mathematical and scheme method, the selection of parameters for obtaining the optimal flexural properties has been proposed. Originality/value The paper presents a new mathematical model for the calculation of the processing parameters in SLS of polymer products and gives a diagram for the determination of which parameters are the best to use for practical purposes.
Sensitization of Lanthanide Luminescence in Heterotrinuclear PtLn<sub>2</sub>(Ln = Eu, Nd, Yb) Complexes with Terpyridyl-Functionalized Alkynyl by Energy Transfer from a Platinum(II) Alkynyl Chromophore (2007)

The preparation and characterization of the mononuclear complexes cis-Pt(dppe)(C⋮CPhtpy)2 (1) and cis-Pt(dppp)(C⋮CPhtpy)2 (5) (dppe = 1,2-bis(diphenylphosphino)ethane, dppp = 1,3-bis(diphenylphosphino)propane, HC⋮CPhtpy = 4‘-(4-ethynylphenyl)-2,2‘:6‘,2‘ ‘-terpyridine) and the corresponding PtLn2 (Ln = Eu, Nd, Yb) heterotrinuclear complexes by incorporating the precursor 1 or 5 with Ln(hfac)3(H2O)2 are described. 1 and 5 exhibit intense, long-lived room-temperature phosphorescence, originating probably from an admixture of 3ILCT (π → π*(C⋮Cphtpy)) and 3MLCT (d(Pt) → π*(C⋮CPhtpy)) triplet states, which qualifies them as favorable energy donors to facilitate Pt → Ln energy transfer in the corresponding PtLn2 heterotrinuclear complexes. As anticipated, sensitized lanthanide luminescence in PtLn2 heterotrinuclear complexes is attained through efficient energy transfer from PtII alkynyl chromophore based 3MLCT and 3ILCT triplet states.
Optical computing: light at the end of the tunnel? (1990)

AT&T Bell Laboratories in New Jersey, USA, have recently publicized the operation of an experimental digital optical processor, which handles information using light rather than electrons. This announcement came at around the same time as researchers from Heriot. Watt University described similar advances in their digital optics programme at the recent conference on optoelectonics and lasers (OE-LASE) in Los Angeles.
Structure and Function of SET and MYND Domain-Containing Proteins (2015)

SET (Suppressor of variegation, Enhancer of Zeste, Trithorax) and MYND (Myeloid-Nervy-DEAF1) domain-containing proteins (SMYD) have been found to methylate a variety of histone and non-histone targets which contribute to their various roles in cell regulation including chromatin remodeling, transcription, signal transduction, and cell cycle control. During early development, SMYD proteins are believed to act as an epigenetic regulator for myogenesis and cardiomyocyte differentiation as they are abundantly expressed in cardiac and skeletal muscle. SMYD proteins are also of therapeutic interest due to the growing list of carcinomas and cardiovascular diseases linked to SMYD overexpression or dysfunction making them a putative target for drug intervention. This review will examine the biological relevance and gather all of the current structural data of SMYD proteins.
A Clinically Evaluated Interferometric Continuous-Wave Radar System for the Contactless Measurement of Human Vital Parameters (2019)

Vital parameters are key indicators for the assessment of health. Conventional methods rely on direct contact with the patients' skin and can hence cause discomfort and reduce autonomy. This article presents a bistatic 24 GHz radar system based on an interferometric six-port architecture and features a precision of 1 µm in distance measurements. Placed at a distance of 40 cm in front of the human chest, it detects vibrations containing respiratory movements, pulse waves and heart sounds. For the extraction of the respiration rate, time-domain approaches like autocorrelation, peaksearch and zero crossing rate are compared to the Fourier transform, while template matching and a hidden semi-Markov model are utilized for the detection of the heart rate from sphygmograms and heart sounds. A medical study with 30 healthy volunteers was conducted to collect 5.5 h of data, where impedance cardiogram and electrocardiogram were used as gold standard for synchronously recording respiration and heart rate, respectively. A low root mean square error for the breathing rate (0.828 BrPM) and a high overall F1 score for heartbeat detection (93.14%) could be achieved using the proposed radar system and signal processing.
Switching with the mercury-wetted-contact relay (1976)

The true essence of worth in any component (electrical, mechanical or solid state) can be illustrated by the length of time the particular device has been in use. Although the passage of time sees the introduction of many new devices, in the main, industry will continue to use components of proven reliability in many applications unless the new product demonstrates clearly its superiority in a number of spheres—cost, reliability, size, availability etc.
Comparison of the cleavage of pyrimidine dimers by the bacteriophage T4 and Micrococcus luteus UV-specific endonucleases. (1980)

A comparison was made of the activity of the UV-specific endonucleases of bacteriophage T4 (T4 endonuclease V) and of Micrococcus luteus on ultravilet light-irradiated DNA substrates of defined sequence. The two enzymes cleave DNA at the site of pyrimidine dimers with the same frequency. The products of the cleavage reaction are the same, suggesting that the scission of DNA by T4 endonuclease V occurs via the combined actin of a pyrimidine dimer specific DNA glycosylase and an apyrimidinic-apurinic (AP) endonuclease as was recently shown for the M. luteus enzyme. The pyrimidine dimer DNA-glycosylase activity of both enzymes is more active on double-stranded DNA than it is on single-stranded DNA.
Data provenance assurance in the cloud using blockchain (2017)

Ever increasing adoption of cloud technology scales up the activities like creation, exchange, and alteration of cloud data objects, which create challenges to track malicious activities and security violations. Addressing this issue requires implementation of data provenance framework so that each data object in the federated cloud environment can be tracked and recorded but cannot be modified. The blockchain technology gives a promising decentralized platform to build tamper-proof systems. Its incorruptible distributed ledger/blockchain complements the need of maintaining cloud data provenance. In this paper, we present a cloud based data provenance framework using block chain which traces data record operations and generates provenance data. We anchor provenance data records into block chain transactions, which provide validation on provenance data and preserve user privacy at the same time. Once the provenance data is uploaded to the global block chain network, it is extremely challenging to tamper the provenance data. Besides, the provenance data uses hashed user identifiers prior to uploading so the blockchain nodes cannot link the operations to a particular user. The framework ensures that the privacy is preserved. We implemented the architecture on ownCloud, uploaded records to blockchain network, stored records in a provenance database and developed a prototype in form of a web service.
Human monocyte-derived macrophages inhibit HCMV spread independent of classical antiviral cytokines (2018)

Infection of healthy individuals with human cytomegalovirus (HCMV) is usually unnoticed and results in life-long latency, whereas HCMV reactivation as well as infection of newborns or immunocompromised patients can cause life-threatening disease. To better understand HCMV pathogenesis we studied mechanisms that restrict HCMV spread. We discovered that HCMV-infected cells can directly trigger plasmacytoid dendritic cells (pDC) to mount antiviral type I interferon (IFN-I) responses, even in the absence of cell-free virus. In contrast, monocyte-derived cells only expressed IFN-I when stimulated by cell-free HCMV, or upon encounter of HCMV-infected cells that already produced cell-free virus. Nevertheless, also in the absence of cell-free virus, i.e., upon co-culture of infected epithelial/endothelial cells and monocyte-derived macrophages (moMΦ) or dendritic cells (moDC), antiviral responses were induced that limited HCMV spread. The induction of this antiviral effect was dependent on cell-cell contact, whereas cell-free supernatants from co-culture experiments also inhibited virus spread, implying that soluble factors were critically needed. Interestingly, the antiviral effect was independent of IFN-γ, TNF-α, and IFN-I as indicated by cytokine inhibition experiments using neutralizing antibodies or the vaccinia virus-derived soluble IFN-I binding protein B18R, which traps human IFN-α and IFN-β. In conclusion, our results indicate that human macrophages and dendritic cells can limit HCMV spread by IFN-I dependent as well as independent mechanisms, whereas the latter ones might be particularly relevant for the restriction of HCMV transmission via cell-to-cell spread.