gebhart/patrepeval huggingface dataset:
Cloning and Expression of the cDNA for E6-AP, a Protein that Mediates the Interaction of the Human Papillomavirus E6 Oncoprotein with p53 (1993)

The E6 oncoproteins of the cancer-associated or high-risk human papillomaviruses (HPVs) target the cellular p53 protein. The association of E6 with p53 leads to the specific ubiquitination and degradation of p53 in vitro, suggesting a model by which E6 deregulates cell growth control by the elimination of the p53 tumor suppressor protein. Complex formation between E6 and p53 requires an additional cellular factor, designated E6-AP (E6-associated protein), which has a native and subunit molecular mass of approximately 100 kDa. Here we report the purification of E6-AP and the cloning of its corresponding cDNA, which contains a novel open reading frame encoding 865 amino acids. E6-AP, translated in vitro, has the following properties: (i) it associates with wild-type p53 in the presence of the HPV16 E6 protein and simultaneously stimulates the association of E6 with p53, (ii) it associates with the high-risk HPV16 and HPV18 E6 proteins in the absence of p53, and (iii) it induces the E6- and ubiquitin-dependent degradation of p53 in vitro.
Poly-Si thin film transistors fabricated on plastic substrates (2002)

Summary form only given. We report the thin film transistor (TFT) fabrication and laser-doping processes for different low-temperature gate dielectrics and doping techniques. Electrical properties of both TFT and test/calibration devices are presented.
Emphasis on the Properties of Metal‐Containing Zeolites Operating Outside the Comfort Zone of Current Heterogeneous Catalytic Reactions (2020)

The development of catalysts that can operate under exceptionally harsh and unconventional conditions is of critical importance for the transition of the energy and chemicals industries to low-emission and renewable chemical feedstocks. In this review we will highlight materials and more specifically metal-containing zeolite catalysts that have been tested under harsh reaction conditions such as high temperature light alkane conversion and biomass valorization. Particular attention will be given to studies that explore the stability and recyclability of metal-containing zeolite catalysts operating in continuous modes. Metal-containing zeolites are considered as an important class of catalysts operating outside the comfort zone of current heterogeneous catalytic reactions in both gas and liquid phase reactions. The relationship between the properties of the metal-containing zeolite and catalytic performance will be explored.
Characterization of Recombinant HPV6 and 11 E1 Helicases (2001)

To better characterize the enzymatic activities required for human papillomavirus (HPV) DNA replication, the E1 helicases of HPV types 6 and 11 were produced using a baculovirus expression system. The purified wild type proteins and a version of HPV11 E1 lacking the N-terminal 71 amino acids, which was better expressed, were found to be hexameric over a wide range of concentrations and to have helicase and ATPase activities with relatively low values for K(m)(ATP) of 12 microm for HPV6 E1 and 6 microm for HPV11 E1. Interestingly, the value of K(m)(ATP) was increased 7-fold in the presence of the E2 transactivation domain. In turn, ATP was found to perturb the co-operative binding of E1 and E2 to DNA. Mutant and truncated versions of in vitro translated E1 were used to identify a minimal ATPase domain composed of the C-terminal 297 amino acids. This fragment was expressed, purified, and found to be fully active in ATP hydrolysis, single-stranded DNA binding, and unwinding assays, despite lacking the minimal origin-binding domain.
Hydrogen Production with a Simple and Scalable Membraneless Electrolyzer (2016)

Ion-conducting membranes are essential components in many electrochemical devices, but they often add substantial cost, limit performance, and are susceptible to degradation. This work investigates membraneless electrochemical flow cells for hydrogen production from water electrolysis that are based on angled mesh flow-through electrodes. These devices can be fabricated with as few as three parts (anode, cathode, and cell body), reflecting their simplicity and potential for low-cost manufacture. 3D printing was used to fabricate prototype electrolyzers that were demonstrated to be electrolyte agnostic, modular, and capable of operating with minimal product crossover. Prototype electrolyzers operating in acidic and alkaline solutions achieved electrolysis efficiencies of 61.9% and 72.5%, respectively, (based on the higher heating value of H2) when operated at 100 mA cm−2. Product crossover was investigated using in situ electrochemical sensors, in situ imaging, and by gas chromatography (GC). GC analysis found that 2.8% of the H2 crossed over from the cathode to the anode stream under electrolysis at 100 mA cm−2 and fluid velocity of 26.5 cm s−1. Additionally, modularity was demonstrated with a three-cell stack, and high-speed video measurements tracking bubble evolution from electrode surfaces provide valuable insight for the further optimization of electrolyzer design and performance.
Digital reconstruction of nonlinear beam propagation (2008)

We extend the technique of digital holography to the case of propagation through nonlinear media. We experimentally verify the technique by reconstructing nonlinear wave dynamics within a self-defocusing medium and nonlinearly imaging through it.
Diaryl-substituted maleic anhydrides (1990)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDiaryl-substituted maleic anhydridesEllis K. Fields, S. J. Behrend, S. Meyerson, M. L. Winzenburg, B. R. Ortega, and H. K. Hall Jr.Cite this: J. Org. Chem. 1990, 55, 17, 5165–5170Publication Date (Print):August 1, 1990Publication History Published online1 May 2002Published inissue 1 August 1990https://pubs.acs.org/doi/10.1021/jo00304a034https://doi.org/10.1021/jo00304a034research-articleACS PublicationsRequest reuse permissionsArticle Views1180Altmetric-Citations37LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Beyond (2010)

What would it be like to reach into a screen and manipulate or design virtual objects as in real world? We present Beyond, a collapsible input device for direct 3D manipulation. When pressed against a screen, Beyond collapses in the physical world and extends into the digital space of the screen, such that users can perceive that they are inserting the tool into the virtual space. Beyond allows users to directly interact with 3D media, avoiding separation between the users' input and the displayed 3D graphics without requiring special glasses or wearables, thereby enabling users to select, draw, and sculpt in 3D virtual space unfettered. We describe detailed interaction techniques, implementation and application scenarios focused on 3D geometric design and prototyping.
Preparation of soapless latexes by sonification of starch‐based poly(isoprene–<i>co</i>–acrylonitrile) graft reaction mixtures (1979)

Abstract Graft copolymerization of isoprene (IP) and acrylonitrile (AN) onto gelatinized starch (S) and cationic starch having quaternary amine functionality through cerium(IV) initiation gave grafted side chains of poly(IP– co –AN). Grafts of various compositions are obtained by controlling the amounts and ratios of monomers added to starch. IP alone does not homograft onto gelatinized starch at 25° or 50°C by cerium(IV) initiation and requires the presence of an “initiator–monomer” such as AN to obtain copolymer side chains. Although cografting of IP and AN onto starch depends on AN to initiate radical chains, the ratio employed of the two monomers is critical for graft polymerization to occur. For example, at a molar ratio of IP to AN of 1 or greater, little polymer was produced; at molar ratios in the range of 0.4 to 0.67, considerable amounts of polymer were produced; and at a molar ratio of 0.13 or less, polymerization of AN was greatly retarded. Concentration of HNO 3 in the cerium(IV) reagent and reaction temperature also influence the grafting reaction. Lower HNO 3 concentrations favor grafting at 50°C, while higher acid concentrations favor grafting at 25°C. Starch graft reaction mixtures were sonified at 20 kHz to form latexes that air dry to clear pliable films. Poly(IP– co –AN) obtained by acid hydrolysis of the starch portion of the grafts failed to dissolve in either dimethylformamide or benzene, thus indicating presence of crosslinks. S– g –poly(IP– co –AN), having about one third starch and grafted side chains averaging about 2 parts polymerized IP per part of polymerized AN, was masticated on steel rolls at 100°C to a tough pliable film which was subsequently vulcanized to a rubber.
KAPOSI'S SARCOMA-ASSOCIATED HERPESVIRUS ENCODED A FUNCTIONAL CYCLIN (1997)

Background: Kaposi's sarcoma-associated herpesvirus (KSHV) is constantly found in Kaposi's sarcoma lesions and body-cavity based B cell lymphomas in HIV infected patients. An open reading frame, which has a high homology with cellular type D cyclins and virus-encoded cyclin of herpesvirus saimiri (HVS), has been identified from KSHV genomic DNA library. Cellular type D cyclins are key elements regulating Gl/S transition of cell cycle. Deregulated expression of cellular cyclins is associated with some types of abnormal cell proliferation and tumors. Methods: The KSHV v-cyclin was cloned from Kaposi's sarcoma biopsy specimen and compared to cellular type D cyclins and v-cyclin of HVS. KSHV v-cyclin was expressed transiently in COS-1 cells and insect cells and stablely in tetracyclin-inducible Rat-1 cells. Immunoprecipitation and immunoblot were used to demonstrate the association of v-cyclin with cellular cyclin-dependent kinases (cdks). The v-cyclin/cdk complexes were subjected toin vitro kinase assay using histone H1 and GST-Rb as substrate. Cell cycle progression was measured by FASC analysis. Results: KSHV v-cyclin has >30% identity and >50% similarity to v-cyclin of HVS and cellular cyclin D2. KSHV v-cyclin was predominantly associated with cdk6. Cdk6/KSHV v-cyclin complexes strongly phosphorylated histone H1 and GST-Rb in vitro. Expression of KSHV v-cyclin has an advanced effect on Gl/S transition in Rat-1 cells. Conclusion: KSHV v-cyclin resembles the v-cyclin of the T-lymphocyte-transforming HVS in its specificity for association with cdk6 and in its ability to strongly activate cdk6 protein kinase activity. The results demonstrate that KSHV encodes a functional Gl cyclin and this cyclin may play an important role in cell cycle regulation in transformed cells associated with KSHV.
Diagnostic and prognostic role of circulating <i>miR-206</i> in rhabdomyosarcoma patients. (2015)

10038 Background: miR-206 is a muscle-specific microRNA and is much more strongly expressed in rhabdomyosarcoma (RMS) tumors than in non-RMS tumors. Here, we analyzed the diagnostic and prognostic significance of circulating miR-206 values in serum specimens of RMS patients. Methods: Total RNA was extracted from serum samples (200 μL) from 60 patients (28 RMS, 32 non-RMS) and quantified by real-time quantitative RT-PCR. Receiver-operating characteristics (ROC) curves were established to evaluate the diagnostic value. Survival curves for RMS patients were calculated by the Kaplan-Meier method and were compared using the log-rank test. The median follow-up time was 30 months. Serum miR-206 expression level and prognostic factors were evaluated with a univariate analysis. The factors included age, primary tumor size, regional lymph node involvement, distant metastasis, fusion gene status, histologic subtype and primary tumor site. A multivariate analysis was conducted using the Cox proportional hazards regression method. The variables correlated with progression-free survival (PFS) in the univariate analysis were included in the model. Results: Serum miR-206 expression levels were higher in RMS patients than in non-RMS patients (p < 0.001) with an area under the ROC curve of 0.8705 (95% confidence interval [CI], 0.7791-0.962), sensitivity of 0.714 and specificity of 0.938. The cut-off value was 164.1 copies/μl serum. PFS was negatively influenced by a high serum miR-206 expression level (2.5-year PFS; 10.7 % (n = 14) vs 77.9 % (n = 14), p = 0.0019) and the presence of both distant metastasis and locoregional lymph node involvement (2.5-year PFS; 30.9 % (n = 18) vs 80.0 % (n = 10), p = 0.017) in univariate analysis. In the multivariate analysis, a high serum miR-206 expression level had an independent prognostic significance (hazard ratio: 3.50 [95% CI: 1.12-11.0], p = 0.031). Conclusions: miR-206 can be a novel biomarker for both diagnosis and treatment stratification of RMS. The limitations of our study include a retrospective design, small sample size and non-uniform treatment. The Japan Rhabdomyosarcoma Study Group will prospectively validate its prognostic significance in a large cohort of uniformly-treated patients.
Optimisation of the pheromone blend of the swede midge, <i>Contarinia nasturtii</i>, for monitoring (2009)

Pheromone traps with the synthetic pheromone in a 1:2:0.02 ratio emitted from PE dispensers were highly effective and long lasting. As the mixture of stereoisomers of 2,10-diacetoxyundecane strongly inhibited attraction of male C. nasturtii while those of 2,9-diacetoxyundecane and 2-acetoxyundecane did not have any inhibitory effect, it is possible to produce traps that are effective and long lasting but cheaper to produce and maintain.
Effect of Third Elements on High-Temperature Strength of TiAl Base Alloys (1993)

After the heat-treatment of 1473 K-10.8 ks, the yield stress of TiAl(γ) base alloys, Ti-50 mol%Al, Ti-(50-C)mol%Al-C mol%X, and Al-(50-C)mol%Ti-C mol%X, was examined by the compression test as a function of temperature (295-1273 K) and the amount, C(0.5∼2.0), of third elements, X(Cr, Ga, Zr, Nb, Sb, Hf, Pd, Pt, Ta). Micro-hardnesses of γ in the alloys were measured at room temperature to confirm solid solution hardening. The microstructures were examined using OM, SEM, EPMA and XRD. The results obtained were examined with regression analysis and compared with the microstructures.The micro-hardness increased with the amount of additives. The increase was remarkable with increasing difference in atomic radius between Ti or Al and X atoms. In the temperature range of 295-1073 K, the yield stress is in proportion to the micro-hardness and influenced by the amount of Ti3Al phase and the grain size of γ phase. At 1273 K, the effect of the microstructure on the yield stress decreased and the effect of solid solution hardening become prominent.
A novel mutation and novel features in Nijmegen breakage syndrome (2001)

Editor—Nijmegen breakage syndrome (NBS) is a rare autosomal recessive disorder, characterised by microcephaly, bird-like face, growth retardation, immunodeficiency, cytogenetic abnormalities, increased radiosensitivity, and high susceptibility to lymphoid malignancy. The NBS1 gene, mapped on chromosome 8q211 and recently cloned,2 3codes for nibrin, a member of the hMre11/hRAD50 protein complex, involved in DNA double strand break repair. The NBS Registry in Nijmegen includes 55 patients. The majority of them are of eastern European origin and share a common haplotype, suggesting a founder effect, and a mutation consisting of a truncating 5 bp deletion in exon 6, 657-661 del ACAAA.4 Five further mutations have been found in six patients with different haplotypes and of various ethnic origins.

We found a new mutation of the NBS1 gene in a 2 year old girl from Morocco. The patient, a girl born at term in August 1997 (fig 1), is the third child of apparently non-consanguineous parents; the two brothers, aged 12 and 6 years, are healthy. The pregnancy was uneventful until the 33rd week, when growth retardation, dilatation of the cerebral ventricles, and agenesis of the corpus callosum was diagnosed by ultrasound examination.



Figure 1 
(A) The patient at 18 months. (B) The right hand with preaxial polydactyly.



Birth weight was 2520 g (3rd centile), length 46 cm (<3rd centile), and head circumference 28.5 cm (<3rd centile). Physical examination showed a “bird-like” face with strabismus, downward slanting palpebral fissures, large and low set dysmorphic ears, and a high palate. The hands showed right preaxial polydactyly and bilateral clinodactyly of the fifth finger with a short middle phalanx and single flexion crease. The feet showed bilateral cutaneous syndactyly 4-5. Severe gastro-oesophageal reflux was also present. Normal findings were obtained on ultrasound examination of the heart and abdominal structures including the ovaries. Agenesis …
On Driving Techniques for LEDs: Toward a Generalized Methodology (2009)

LEDs must be externally driven by power sources to emit light. One problem associated with driving LEDs is its inherent nonlinear relation between the emission intensity and the forward current. Thus, the light output obtained from an LED is strongly dependent on the actual current waveforms employed to drive it. It is found that driving an LED with dc produces light output that surpasses all other techniques including the commonly used pulse-width modulation (PWM) technique. On the other hand, for dimming function, it is found that the PWM technique offers greater dimming flexibility in comparison to dc technique. In this paper, a generalized methodology for driving LEDs inheriting the features of both of these techniques is proposed. It employs a pulsating current switching between two discrete current levels, where the current levels and their durations can be concurrently varied for a more precise mapping of the driving conditions to the light output. The existing dc and PWM techniques can be viewed alternatively as being special cases of this more general approach.
DD 21A-A Capable, Affordable, Modular 21st Century Destroyer (1993)

Future Navy ships must be superior but inexpensive. A new philosophy and configuration provide the 21st century destroyer, the DD 21A, with global range; reduced lightship displacement and cost; superior seakeeping; no seawater ballast; sharper turns and stops; and greatly reduced installed power, fuel consumption, and pollution. These benefits result from a new machinery-driven ship design paradigm centered on simplicity and efficiency. All main machinery is modular and outside the watertight hull, freeing midship areas for personnel. The tumble home (inward-sloped) hull is long and slender, requiring little power at maximum speed. Two removable, prealigned and pretested propulsor modules are attached to the stern after hull construction and are replaceable pierside. Each module includes a steerable pod aligned to the water inflow. A streamlined strut connects each pod rigidly to a vertical steerable barrel. Two removable, power- producing modules are mounted in the helicopter hangar. Each module comprises a 26,400-hp (19.7-MW) intercooled, recuperated gas turbine; a 4-MW ship service alternator; and a 20-MW propulsion alternator. These remarkable results are obtained by taking a reference destroyer from the advanced surface ship evaluation tool data bank and evaluating several progressive changes made to it.
PHOTOSENSITIZED OXYGENATION OF ALDEHYDE–AMINE MIXTURE. FACILE CARBONYL TRANSFORMATION FROM ALDEHYDE TO AMINE INVOLVING RECYCLIZATION PROCESS (1977)

Abstract Photosensitized oxygenation of mixture of various aldehydes and morpholine(piperidine) gave N-formylmorpholine(N-formylpiperidine). When long chain non-branched aldehydes were used, the N-formylamine was obtained in more than 100% yield on the basis of aldehyde. This effective carbonyl transfer to amine includes reproduction of aldehyde.
A fully-integrated 1.8-V, 2.8-W, 1.9-GHz, CMOS power amplifier (2003)

This paper demonstrated the first 2-stage, 2.8 W, 1.8 V, 1.9 GHz fully-integrated distributed active transformer (DAT) power amplifier with 50 /spl Omega/ input and output matching using 0.18 /spl mu/m CMOS transistors. It has a small-signal gain of 27 dB. The amplifier provides 2.8 W of power into a 50 /spl Omega/ load with a PAE of 50%.
Fetal globin gene inducers: novel agents and new potential (2010)

Inducing expression of endogenous fetal globin (gamma-globin) gene expression to 60-70% of alpha globin synthesis produces beta-thalassemia trait globin synthetic ratios and can reduce anemia to a mild level. Several classes of therapeutics have induced gamma-globin expression in beta-thalassemia patients and subsequently raised total hemoglobin levels, demonstrating proof-of-concept of the approach. Butyrate treatment eliminated transfusion requirements in formerly transfusion-dependent patients with treatment for as long as seven years. However, prior generation inducers were not readily applicable for widespread use. Currently, a novel oral dual-action therapeutic, sodium 2,2-dimethylbutyrate, is in clinical trials, an oral decitabine formulation is under development, and agents with complementary mechanisms of action can be applied in combined regimens. Identification of three major genetic trait loci which modulate clinical severity provides avenues for developing tailored regimens. These refinements offer renewed potential to apply fetal globin induction as a treatment approach in patient-friendly regimens that can be used worldwide.
Chemically Modified, α-Amino-3-hydroxy-5-methyl-4-isoxazole (AMPA) Receptor RNA Aptamers Designed for<i>in Vivo</i>Use (2017)

Glutamate ion channels have three subtypes, that is, α-amino-3-hydroxy-5-methyl-4-isoxazole (AMPA), kainate, and N-methyl-d-aspartate (NMDA) receptors. Excessive activity of these receptor subtypes either individually or collectively is involved in various neurological disorders. RNA aptamers as antagonists of these receptors are potential therapeutics. For developing aptamer therapeutics, the RNA aptamers must be chemically modified to become ribonuclease-resistant or stable in biological fluids. Using systematic evolution of ligands by exponential enrichment (SELEX) and a chemically modified library, prepared enzymatically (i.e., the library contains RNAs with 2'-fluoro modified nucleoside triphosphates or ATPs, CTPs and UTPs, but regular GTPs), we have isolated an aptamer. The short aptamer (69 nucleotides) FN1040s selectively inhibits the GluA1 and GluA2Q<sub>flip</sub> AMPA receptor subunits, whereas the full-length aptamer (101 nucleotides) FN1040 additionally inhibits GluK1, but not GluK2, kainate receptor, and GluN1a/2A and GluN1a/2B, the two major native NMDA receptors. The two aptamers show similar potency (2-4 μM) and are stable with a half-life of at least 2 days in serum-containing medium or cerebrospinal fluid. Therefore, these two aptamers are amenable for in vivo use.