gebhart/patrepeval huggingface dataset:
Overexpression of the MexEF-OprN Multidrug Efflux System Affects Cell-to-Cell Signaling in <i>Pseudomonas aeruginosa</i> (2001)

Intrinsic and acquired antibiotic resistance of the nosocomial pathogen Pseudomonas aeruginosa is mediated mainly by the expression of several efflux pumps of broad substrate specificity. Here we report that nfxC type mutants, overexpressing the MexEF-OprN efflux system, produce lower levels of extracellular virulence factors than the susceptible wild type. These include pyocyanin, elastase, and rhamnolipids, three factors controlled by the las and rhl quorum-sensing systems of P. aeruginosa. In agreement with these observations are the decreased transcription of the elastase gene lasB and the rhamnosyltransferase genes rhlAB measured in nfxC type mutants. Expression of the lasR and rhlR regulator genes was not affected in the nfxC type mutant. In contrast, transcription of the C4-homoserine lactone (C4-HSL) autoinducer synthase gene rhlI was reduced by 50% in the nfxC type mutant relative to that in the wild type. This correlates with a similar decrease in C4-HSL levels detected in supernatants of the nfxC type mutant. Transcription of an rhlAB-lacZ fusion could be partially restored by the addition of synthetic C4-HSL and Pseudomonas quinolone signal (PQS). It is proposed that the MexEF-OprN efflux pump affects intracellular PQS levels.
Real-time optical-fibre luminescence dosimetry for radiotherapy: physical characteristics and applications in photon beams (2004)

A new optical-fibre radiation dosimeter system, based on radioluminescence and optically stimulated luminescence from carbon-doped aluminium oxide, was developed and tested in clinical photon beams. This prototype offers several features, such as a small detector (1 x 1 x 2 mm3), high sensitivity, real-time read-out and the ability to measure both dose rate and absorbed dose. The measurements describing reproducibility and output dependence on dose rate, field size and energy all had standard deviations smaller than 1%. The signal variation with the angle of incidence was smaller than 2% (1 SD). Measurements performed in clinical situations suggest the potential of using this real-time system for in vivo dosimetry in radiotherapy.
ChemInform Abstract: Ring Transformations (1997)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Association between acute and chronic graft-versus-host disease and bronchiolitis obliterans organizing pneumonia in recipients of hematopoietic stem cell transplants (2003)

Bronchiolitis obliterans organizing pneumonia (BOOP) has been reported following hematopoietic stem cell (HSC) transplantation, but the clinical features and risk factors for this disorder have not been well characterized. This case-control study of 49 patients with histologic BOOP and 161 control subjects matched by age and year of transplantation describes the clinical features and analyzes the risk factors for BOOP following HSC transplantation. Data on clinical features and outcome were collected by chart review. Odds ratios, estimating the relative risk of BOOP in allogeneic HSC recipients, were calculated by conditional logistic regression with adjustment for potential confounding factors. Clinical features of BOOP in this population were similar to idiopathic BOOP and BOOP occurring in other disease settings. There was an association between acute and chronic graft-versus-host disease (GVHD) and the subsequent development of BOOP (odds ratios, 3.8 [95% CI, 1.2 to 12.3] and 3.1 [95% CI, 1.1 to 9.2], respectively). Patients with BOOP were more likely to have acute GVHD involving the skin (odds ratio, 4.6; P =.005) and chronic GVHD involving the gut (odds ratio, 6.6; P =.018) and oral cavity (odds ratio, 5.9; P =.026). This study shows that histologic BOOP following HSC transplantation has clinical features that resemble idiopathic BOOP and is strongly associated with prior acute and chronic GVHD. These results have important implications for the care of patients who develop respiratory symptoms after HSC transplantation and may help elucidate the pathogenesis of idiopathic BOOP.
<i>Panax notoginseng</i> Saponins Promotes Stroke Recovery by Influencing Expression of Nogo-A, NgR and p75NGF, <i>in Vitro</i> and <i>in Vivo</i> (2014)

The spontaneous recovery of function after injury in the adult central nervous system is limited due to the several proteins, such as Nogo-A that have repulsive or inhibitory effects on growing neuritis. The Chinese herbal medicine extraction Panax notoginseng saponins (PNS) injection has been widely used and effective in repairing the function of impaired nerves, but the mechanism of this herbal medicine is still poorly understood. This project evaluated the effect of Panax notoginseng saponins on neurological functional recovery and on the expression of Nogo-A, NgR and p75 at 7, 14 and 28 d after middle cerebral artery occlusion (MCAO) in rats and also oxygen-glucose deprivation/reperfusion (OGD/R) model on SH-SY5Y cells. We found that the expression of Nogo-A, NgR and p75 of rats receiving MCAO surgery increased on the 7th day, reached a peak on the 14th or 28th day and maintained high levels and Panax notoginseng saponins significantly decreased these expressions. This may be the mechanism of Panax notoginseng saponins that contributes to the recovery of nerve function, which plays an important role in brain protection after cerebral infarction.
The synthesis and liquid crystal transition temperatures of some weakly polar nematic<i>trans</i>-4-substituted-cyclohexyl (<i>E</i>)-alk-2-enoates (1994)

Abstract The effect on the liquid crystal transition temperatures of introducing various groups (for example incorporating C˭C, O, CO2 and CO) into the terminal alkyl chain of a weakly polar model compound 1-[trans-4-(trans-4-propylcyclohexyl)cyclohexyl]pentane has been investigated systematically. Only the compound containing both an ester function and a trans-carbon-carbon double bond exhibited a wide-range nematic mesophase at elevated temperatures. Therefore, a wide variety of trans-4-substituted-cyclohexyl (E)-alk-2-enoates incorporating a carbon double bond with a trans-configuration (E) in the terminal alkyl chain has been synthesized. Nearly all the two-ring esters prepared exhibit a nematic phase over a wide temperature range (≤ 100°C) at elevated temperatures (≤ 200°C). The tendency to form smectic mesophases is often low. Comparisons with the corresponding derivatives incorporating either just a carboxy group (COO) or just a carbon-carbon double bond (C˭C) in the same positions indicate that synergetic effects lead to broader nematic phases than would otherwise have been expected. The new compounds are easily prepared from known starting materials.
The Need for Caution With Topical Anesthesia During Endoscopic Procedures, as Liberal Use May Result in Methemoglobinemia (2004)

During upper gastrointestinal endoscopy, topical oropharyngeal anesthesia with lidocaine and/or benzocaine is used routinely by many endodscopists. Although such a practice is usually safe, there have been a number of reports of methemoglobinemia induced by topical anesthesia. Early treatment is extremely important as the development of methemoglobinemia is potentially fatal. Methemoglobinemia should be considered when oxygen desaturation occurs without another explanation. In this case series, we report 4 cases of methemoglobinemia that followed the liberal application of Cetacaine for ERCP. All patients recovered after appropriate treatment but these cases serve to highlight the potential problem, the importance of early recognition and treatment, and the most appropriate treatment options.
Multi-voltage device integration technique for 0.5 μm BiCMOS and DMOS process (2002)

This paper describes the multi-voltage device integration technique for BiCMOS and DMOS process and the simple measures to the beta decreasing of 5 V/12 V NPNTr by the lack of heat treatment used for half-micron process. The N well offset gate are used for 30 V HV-NMOS. Moreover, RESURF effect by Buried P+ is applied to 60 V HV-NMOS. Each specific on-resistance (Ron/spl middot/S) reaches sufficiently low value (60 m /spl Omega/ mm2 (BVds=33 V), 127 m /spl Omega/ mm2 (BVds=74 V)). 60 V DMOS structure became to 90 V DMOS by only addition of P well guard ring, and the Ron/spl middot/S is 230 m /spl Omega/ mm2 (BVds=94 V). Using the appropriate length of RESURF formed at N-epitaxial layer; we could optimize 30 V/60 V/90 V NMOS devices and FID (Full Isolation Diode). In the case of PMOS, 30 V/60 V/90 V PMOS has been realized by using the combination of P-LDD (Lightly Doped Drain) and Sinker P region (P body, P well). Using a P+ shield region stabilized the beta of 5 V/12 V NPNTr. Any complicated process step is not needed for various techniques mentioned above.
Reactions of 2‐amino‐, 2‐alkylamino‐, and 2‐piperidino‐1‐azaazulenes with aryl and chlorosulfonyl isocyanates (1996)

Abstract Reaction of 2‐amino‐1‐azaazulene with phenyl isocyanate gave 3‐phenyl‐2 H ‐3,4‐dihydro‐1,3,4a‐triazabenz[5,4‐ a ]azulene‐2,4‐dione. Reactions of 2‐alkylamino‐1‐azaazulenes with aryl isocyanates gave 2‐( N ‐ethyl‐ N ′‐arylureido)‐1‐azaazulenes initially, which rearranged to N ‐aryl‐2‐alkylamino‐1‐azaazulene‐3‐carboxamides and successive reaction with another molar amount of aryl isocyanate furnished uracil‐fuzed 1‐azaazulenes. Reaction of 2‐piperidino‐1‐azaazulene with aryl isocyanate gave N ‐aryl‐2‐piperidino‐1‐azaazulene‐3‐carboxamide. Reaction of 2‐(substituted amino)‐1‐azaazulenes with chlorosulfonyl isocyanate gave 3‐cyano‐ and 3‐chloro‐2‐(substituted amino)‐1‐azaazulenes.
Extrusion of fused silica cladding tubes (1981)

The first step in producing optical waveguides by the MCVD process1 is making highly concentric fused silica tubes with minimal variation along their entire length. These are among the tube properties required to make them compatible with subsequent process steps and end-product requirements. Drawing processes are presently used to fabricate these tubes where their final shape is formed in free space under the guidance of the drawing parameters. Extrusion methods make use of an orifice to control the final shape of the issuing extrudate. It is this inherent process property that was the impetus to explore the extrusion of fused silica tubing. This paper describes a gas-head extrusion method for fabrication of pure fused silica tubes.
Interleukin-10: An Important Immunoregulatory Cytokine With Major Impact on Psoriasis (2004)

Interleukin (IL)-10 is a pluripotent cytokine with effects on numerous cell populations, in particular circulating and resident immune cells as well as epithelial cells. With its potent immunoregulatory capacities its main biological function seems to be the limitation and termination of inflammatory responses. Thus its low level expression found in psoriasis may have pathophysiological relevance for this immune disease. Remarkably, induction of IL-10 expression was found by conventional antipsoriatic therapies, supporting the hypothesis that IL-10 may be a key cytokine in psoriasis and that application of this cytokine itself may have therapeutic effects. In first clinical trials in patients with established psoriasis IL-10 showed moderate antipsoriatic effects and was well tolerated. Moreover, long term application in psoriatic patients remission showed that IL-10 therapy decreases the incidence of relapse and prolongs the disease free interval. The immunological effects observed during these clinical studies together with in vitro observations suggests that IL-10 exerts its antipsoriatic activity by effects on different cell populations including antigen presenting cells and T-cells (lasting type 1/ type 2 cytokine balance shift), but not through direct effects on keratinocytes. In conclusion IL-10 seems to have major importance in psoriasis. Further investigations, in particular multicenter, placebo-controlled, double blind trials are required to fully determine whether IL-10 application will become a successful antipsoriatic therapy.
Antrodia cinnamomea induces autophagic cell death via the CHOP/TRB3/Akt/mTOR pathway in colorectal cancer cells (2018)

Antrodia cinnamomea, a well-known traditional medicine used in Taiwan, is a potent anticancer drug for colorectal cancer, but the upstream molecular mechanism of its anticancer effects remains unclear. In this study, A. cinnamomea extracts showed cytotoxicity in HCT116, HT29, SW480, Caco-2 and, Colo205 colorectal cancer cells. Whole-genome expression profiling of A. cinnamomea extracts in HCT116 cells was performed. A. cinnamomea extracts upregulated the expression of the endoplasmic reticulum stress marker CHOP and its downstream gene TRB3. Moreover, dephosphorylation of Akt and mTOR as well as autophagic cell death were observed. Gene expression and autophagic cell death were reversed by the knockdown of CHOP and TRB3. Autophagy inhibition but not apoptosis inhibition reversed A. cinnamomea-induced cell death. Finally, we demonstrated that A. cinnamomea extracts significantly suppressed HCT116 tumour growth in nude mice. Our findings suggest that autophagic cell death via the CHOP/TRB3/Akt/mTOR pathway may represent a new mechanism of anti-colorectal cancer action by A. cinnamomea. A. cinnamomea is a new CHOP activator and potential drug that can be used in colorectal cancer treatment.
Extraction and Separation of Fucoidan from<i>Laminaria japonica</i>with Chitosan as Extractant (2013)

Herein the extraction method of fucoidan from Laminaria japonica is reported. Firstly, chitosan, chitosan-N-2-hydroxypropyl trimethyl ammonium chloride (HACC), and hexadecyltrimethylammonium bromide (CPAB) were used to extract the fucoidan. The results showed that chitosan was the optimal extractant compared with the other two extractants. After extraction, different aqueous solutions, including NaCl, KCl, and HCl (pH2), were used to separate fucoidan from chitosan-fucoidan complex. The results showed that the separation ability of NaCl was slightly higher than that of KCl. Moreover, the price of NaCl is lower than that of KCl. Given the quality-price rate, NaCl solution was chosen as the separation solution. Thirdly, the concentration and ratio of NaCl solution : sediment influence the separation of fucoidan from chitosan-fucoidan complex. The results showed that the optimal separation conditions include 4 mol/L NaCl solution with the ratio of NaCl solution to sediment at 30 : 1. Fucoidan content was found to be affected by different separation time. Fucoidan content increased with the increase of separation time, and the optimal separation time was 6 h. Compared with traditional alkali extraction method, this method not only reduces the usage of alkali and acid and alleviate environment pollution, but also has the comparable extraction yield of fucoidan. It is a potential method for extraction of fucoidan.
Compressing spin-polarized 3He with a modified diaphragm pump (2001)

Nuclear spin-polarized (3)He gas at pressures on the order of 100 kPa (1 bar) are required for several applications, such as neutron spin filters and magnetic resonance imaging. The metastability-exchange optical pumping (MEOP) method for polarizing (3)He gas can rapidly produce highly polarized gas, but the best results are obtained at much lower pressure (~0.1 kPa). We describe a compact compression apparatus for polarized gas that is based on a modified commercial diaphragm pump. The gas is polarized by MEOP at a typical pressure of 0.25 kPa (2.5 mbar), and compressed into a storage cell at a typical pressure of 100 kPa. In the storage cell, we have obtained 20 % to 35 % (3)He polarization using pure (3)He gas and 35 % to 50 % (3)He polarization using (3)He-(4)He mixtures. By maintaining the storage cell at liquid nitrogen temperature during compression, the density has been increased by a factor of four.
Smoothed Particle Hydrodynamics and Its Diverse Applications (2011)

This review focuses on the applications of smoothed particle hydrodynamics (SPH) to incompressible or nearly incompressible flow. In the past 17 years, the range of applications has increased as researchers have realized the ability of SPH algorithms to handle complex physical problems. These include the disruption of free surfaces when a wave hits a rocky beach, multifluid problems that may involve the motion of rigid and elastic bodies, non-Newtonian fluids, virtual surgery, and chemical precipitation from fluids moving through fractured media. SPH provides a fascinating tool that has some of the properties of molecular dynamics while retaining the attributes of the macroscopic equations of continuum mechanics.
Intracorneal Inlays for the Correction of Ametropias (2015)

Various materials have been placed in the corneal stroma to modify a spherical refractive error (usually hyperopic). As the surgical procedures evolved to simplify the insertion process so has the quality and biocompatibility of the implanted materials. Failures have been due to excessive inlay dimensions, bioincompatibility of the materials used, poor choice of test model, or combinations of the above. We present a thorough analysis of the history of the intracorneal inlay and the materials and techniques used up to and including the current materials and techniques available for the correction of spherical refractive errors.
High-birefringence fiber loop mirrors and their applications as sensors (2005)

The reflection and transmission characteristics of a high-birefringence fiber loop mirror (HiBi-FLM), which is composed of a standard fiber coupler and one-section or multisection high-birefringence fibers (HBFs), are analyzed and discussed in detail. Theoretical reflectivity and transmissivity expressions for HiBi-FLMs with one-, two-, and three-section HBFs were obtained. The procedure for calculating reflectivity and transmissivity for HiBi-FLMs with n-section HBFs is given. Experimental results have verified the theoretical model. The basic characteristics of the one-section HiBi-FLM when strain and high temperature are applied to HBFs were analyzed and investigated theoretically and experimentally. The experimental results are in good agreement with the theoretical analysis. Furthermore, a strain--temperature sensor that makes use of those characteristics, which is new for applications of HiBi-FLMs, has been proposed and demonstrated.
Ventricular assist device selection: which one and when? (2014)

Advances in mechanical circulatory support have significantly expanded the treatment options for patients with heart failure, whether acute or chronic. There are numerous devices available that offer patients short-, intermediate-, and long-term duration of support depending on their clinical needs and cardiac recovery. Each device has its own technical considerations and the decision which device to use depends on several factors, including what is available, the degree of support required, and expected duration of support. Additional issues that need to be considered in choosing level of support include right heart function, respiratory failure, and multi-organ derangements. A widespread availability of short-term ventricular assist devices and timely institution for effective hemodynamic support will translate into improved patient outcomes whether that is successful transfer to a tertiary care facility or recovery of inherent cardiac function. Implantable ventricular assist devices have and will continue to evolve into smaller and more durable devices, and the future for patients with advanced heart failure looks ever-more promising.
The Synthesis of β-Benzyl L- Aspartate and γ-Benzyl L-Glutamate by Enzyme-Catalyzed Hydrolysis (1987)

The enzyme-catalyzed hydrolysis of dibenzyl ester of aspartic acid and glutamic acid in α-position to give better yield of β-benzyl aspartate and γ-benzyl glutamate than the chemical method is reported.
Mechanisms of inherited deficiencies of multiple UDP‐glucuronosyltransferase isoforms in two patients with Crigler‐Najjar syndrome, type I (1992)

Crigler-Najjar syndrome, type I (CN-I) is a potentially lethal disorder characterized by severe unconjugated hyperbilirubinemia resulting from a recessively inherited deficiency of hepatic UDP-glucuronosyl-transferase (UGT) activity toward bilirubin (B-UGT). Two forms of B-UGT exist in human liver. mRNAs for these two forms and that for another isoform with activity toward simple phenols (P-UGT) have unique 5' regions, but their 3' regions are identical. The three mRNA species are derived from a single locus; the unique 5' regions are encoded by single unique exons and the identical 3' regions consist of four consecutive exons that are shared by all three isoforms. In this paper, we determined genetic lesions in two CN-I patients with deficiency of hepatic B-UGT and P-UGT activities. In one patient, there was a C----T substitution in exon 4 (common region) predicting the substitution of a serine residue with a phenylalanine residue; this mutation was present in the identical region of B-UGT and P-UGT mRNAs. In the other patient, a C----T substitution in exon 2 (common region) of the B-UGT/P-UGT locus resulted in a premature stop codon. This exon (132 nt) was absent in heptic B-UGT and P-UGT mRNAs of this patient due to exon skipping during pre-mRNA processing. Sequence abnormality of three distinct mRNA species explains the abnormality of multiple UGT isoforms in these patients. Presence of identical abnormalities in the common regions of the three mRNAs is consistent with the finding that the common 3' regions of the two B-UGT mRNAs and the P-UGT mRNA are encoded by four shared exons.
Structure and Biodegradation Mechanism of Milled Bombyx mori Silk Particles (2012)

The aim of this study was to understand the structure and biodegradation relationships of silk particles intended for targeted biomedical applications. Such a study is also useful in understanding structural remodelling of silk debris that may be generated from silk-based implants. Ultrafine silk particles were prepared using a combination of efficient wet-milling and spray-drying processes with no addition of chemicals other than those used in degumming. Milling reduced the intermolecular stacking forces within the β-sheet crystallites without changing the intramolecular binding energy. Because of the rough morphology and the ultrafine size of the particles, degradation of silk particles by protease XIV was increased by about 3-fold compared to silk fibers. Upon biodegradation, the thermal degradation temperature of silk increased, which was attributed to the formation of tight aggregates by the hydrolyzed residual macromolecules. A model of the biodegradation mechanism of silk particles was developed based on the experimental data. The model explains the process of disintegration of β-sheets, supported by quantitative secondary structural analysis and microscopic images.