gebhart/patrepeval huggingface dataset:
Highly specific enrichment of phosphopeptides by zirconium dioxide nanoparticles for phosphoproteome analysis (2007)

Large-scale characterization of phosphoproteins requires highly specific methods for the purification of phosphopeptides because of the low abundance of phosphoproteins and substoichiometry of phosphorylation. A phosphopeptide enrichment method using ZrO2 nanoparticles is presented. The high specificity of this approach was demonstrated by the isolation of phosphopeptides from the digests of model phosphoproteins. The strong affinity of ZrO2 nanoparticles to phosphopeptides enables the specific enrichment of phosphopeptides from a complex peptide mixture in which the abundance of phosphopeptides is two orders of magnitude lower than that of nonphosphopeptides. Superior selectivity of ZrO2 nanoparticles for the enrichment of phosphorylated peptides than that of conventional immobilized metal affinity chromatography was observed. Femtomole phosphopeptides from digestion products could be enriched by ZrO2 nanoparticles and can be well detected by MALDI mass spectrometric analysis. ZrO2 nanoparticles were further applied to selectively isolate phosphopeptides from the tryptic digestion of mouse liver lysate for phosphoproteome analysis by nanoliter LC MS/MS (nano-LC-MS/MS) and MS/MS/MS. A total of 248 defining phosphorylation sites and 140 phosphorylated peptides were identified by manual validation using a series of rigid criteria.
High pulse energy passive Q-switching of a diode-pumped Tm:YLF laser by Cr:ZnSe (2015)

A passively Q-switched diode-pumped Tm:YLF laser with polycrystalline Cr:ZnSe as the saturable absorber is demonstrated for the first time, to the best of our knowledge. By using saturable absorbers with different initial transmission, the maximum pulse energy reached 4.22 mJ with peak power of 162.3 kW for a pulse duration of 26 ns. The maximum output average power amounted to 2.2 W. These results constitute significant improvement from the highest average power, pulse energy and peak power results for the PQS Tm:YLF laser to date.
Investigation and simulation model results of high density wireless power harvesting and transfer method (2017)

The paper presents and provide simulation model results of a method for wireless power transfer by harvesting and/or transferring the switching ripple energy of the inductor current in a switching power converter. By placing a planar coil as a wireless power receiver (Rx) in the proximity of a power inductor of a power converter, wireless power can be received by the Rx coil because of the oscillating magnetic field generated by AC switching ripple of the inductor current. This yields a power converter with a wireless power transmitter (Tx) in addition to the original wired output of the power converter, therefore, eliminating the need for Tx side in an inductive wireless power transfer system. Using an example Buck-WPT system, which achieves step down power conversion for a wired output and WPT at the same time, this paper discusses the related operation principle and provide simulation model results.
Via header field parameter to indicate received realm (2014)

This specification defines a new Session Initiation Protocol (SIP) Via
header field parameter, received-realm, which allows a SIP entity
acting as an entry point to a transit network to indicate from which
adjacent upstream network a SIP request is received, using a network
realm value associated with the adjacent network.
Constant-Amplitude Biorthogonal Multi-Code Modulation (2005)

In this paper, we propose a biorthogonal modulation employing a constant-amplitude transmission of multi-code signals by introducing a simple constant-amplitude coding scheme with redundant bits. The proposed constant-amplitude biorthogonal multi-code (CABM) modulation can provide high spectral efficiency compared with a conventional direct sequence/spread spectrum (DS/SS) modulation. Nevertheless the proposed CABM modulation can keep up a constant-amplitude signal. Additionally, we propose various types of demodulation structures for the CABM modulation. Simulation results show that bit error rate (BER) performance and hardware complexity of the proposed CABM modulation are highly improved in comparison with those of a constant-amplitude orthogonal multi-code (CAOM) modulation.
Magnetic and Morphological Properties of Ferrofluid-Impregnated Hydroxyapatite/Collagen Scaffolds (2014)

In this article we present the morphological and magnetic characterization of ferrofluid-impregnated biomimetic scaffolds made of hydroxyapatite and collagen used for bone reconstruction. We describe an innovative and simple impregnation process by which the ferrofluid is firmly adsorbed onto the hydroxyapatite/collagen scaffolds. The process confers sufficient magnetization to attract potential magnetic carriers, which may be used to transport bioactive agents that favour bone regeneration. The crystalline structure of the magnetite contained in the ferrofluid is preserved and its quantity, estimated from the weight gain due to the impregnation process, is consistent with that obtained from energy dispersive X-ray spectroscopy. The magnetization, measured with a superconducting quantum interference device, is uniform throughout the scaffolds, demonstrating the efficiency of the impregnation process. The field emission gun scanning electron microscopy characterization demonstrates that the process does not alter the morphology of the hydroxyapatite/collagen scaffolds, which is essential for the preservation of their bioactivity and consequently for their effectiveness in promoting bone formation.
Multifocal optical-resolution photoacoustic microscopy in reflection mode (2013)

Compared with single-focus optical-resolution photoacoustic microscopy (OR-PAM), multifocal OR-PAM utilizes both multifocal optical illumination and an ultrasonic array transducer, significantly increasing the imaging speed.Here we present a reflection-mode multifocal OR-PAM system based on a microlens array that provides multiple foci and an ultrasonic array transducer that receives the excited photoacoustic waves from all foci simultaneously.By using a customized microprism to reflect the incident laser beam to the microlens array, we align the multiple optical foci confocally with the focal zone of the ultrasonic array transducer.Experiments show our reflection-mode multifocal OR-PAM system is capable of imaging microvessels in vivo, and it can image a 9 mm × 5 mm × 2.5 mm volume at 16 μm lateral resolution in ~4 min, limited by the signal multiplexing ratio and laser pulse repetition rate.
Mesoporous Metal and Metal Alloy Particles Synthesized by Aerosol‐Assisted Confined Growth of Nanocrystals (2012)

From droplets to "spheres": A platform technology enables the rapid and continuous synthesis of mesoporous metal and metal alloy particles (see picture). The confined growth of nanocrystals in aerosol droplets leads to the formation of these particles with defined composition.
A divider-less sub-harmonically injection-locked PLL with self-adjusted injection timing (2013)

A low-phase-noise phase-locked loop (PLL) is widely used in clock generation, frequency synthesis, and data conversion. In a PLL using a sub-sampling phase detector (SSPD) achieves not only low phase noise, but also low power. In a low-phase-noise sub-harmonically injection-locked PLL (SIPLL) is presented. The injection timing of a SIPLL is sensitive to the process, voltage, and temperature (PVT) variations. In addition, the divider of a SIPLL [3-5] cannot be powered down to save the power as in [1, 2]. In this paper, a divider-less SIPLL with self-adjusted injection timing is presented.
Combined Stiffness and PDFF Phantom for Quality Assurance of Quantitative Magnetic Resonance Elastography and Fat Fraction Imaging (2023)

MRI proton density fat fraction (PDFF) and MR elastography (MRE) methods are frequently used to evaluate patients with known or suspected NAFLD or NASH. Reference standards (phantoms) are needed for quality assurance of these methods. Currently available phantoms mimic only a single tissue property. A phantom that simultaneously mimics PDFF and tissue stiffness would more realistically mimic NAFLD/NASH tissue and would enable robust and convenient quality assurance. In this work, a chemical formulation and phantom configuration were developed for a multi-layer combined PDFF-MRE phantom, in which each layer simultaneously mimics different PDFF and stiffness values.
Simulation of Cylindrical Plunge Grinding Processes Considered Successive Change of Workpiece Shape (2011)

A simulation method of cylindrical plunge grinding processes is described, which has considered successive change of workpiece shape due to core elements of grinding machine. Each element is modeled and integrated in the simulation procedure. The validity of the method is shown by comparing predicted and experimental results of grinding force histories, workpiece diameter histories and final workpiece shapes. The method is also applied to reduce grinding time by controlling infeed rate. The new process with reduced time is derived and the practicality of the method is verified by conducting experiments.
AB077. P049. Genetic relationship of pancreatic ductal adenocarcinoma and co-occurring IPMN (2018)

Background: Cystic precursor lesions, in particular intraductal papillary mucinous neoplasms (IPMNs), have a significant potential to transform into invasive pancreatic ductal adenocarcinoma (PDAC). After resection, pathological examination of a sizable proportion of invasive PDACs reveal a co-occurring IPMN. Yet, it remains unclear whether the co-occurring IPMN represents a precursor to the invasive PDAC or an unrelated lesion.
Flexible packaging of solid-state integrated circuit chips with elastomeric microfluidics (2013)

A flexible technology is proposed to integrate smart electronics and microfluidics all embedded in an elastomer package. The microfluidic channels are used to deliver both liquid samples and liquid metals to the integrated circuits (ICs). The liquid metals are used to realize electrical interconnects to the IC chip. This avoids the traditional IC packaging challenges, such as wire-bonding and flip-chip bonding, which are not compatible with current microfluidic technologies. As a demonstration we integrated a CMOS magnetic sensor chip and associate microfluidic channels on a polydimethylsiloxane (PDMS) substrate that allows precise delivery of small liquid samples to the sensor. Furthermore, the packaged system is fully functional under bending curvature radius of one centimetre and uniaxial strain of 15%. The flexible integration of solid-state ICs with microfluidics enables compact flexible electronic and lab-on-a-chip systems, which hold great potential for wearable health monitoring, point-of-care diagnostics and environmental sensing among many other applications.
AAV as a Gene Transfer Vector for the Treatment of Neurological Disorders: Novel Treatment Thoughts for ALS (2009)

Therapeutic delivery to the central nervous system has challenged scientists and clinicians due to the difficulty in delivering molecules and genes in an efficient manner across the blood brain barrier (BBB). This has particularly hampered efforts to deliver therapeutics to widely dispersed neurons that perish in diseases such as Amyotrophic Lateral Sclerosis (ALS), a disease affecting motor neurons throughout the brainstem and the entire spinal cord. Gene therapy has offered several potential routes to overcome the difficulties in delivering therapeutics to the brain and spinal cord. Adeno-associated viral vectors (AAV) have taken center stage for gene delivery to the central nervous system, given their ability to express genes in post mitotic cells for long periods with minimal to no toxicity. This review will focus on recent approaches to treat motor neuron disease, in particular ALS using AAV vectors.
P‐3: Organic Thin‐Film Transistors with Solution‐Processed Pentacene and Polysilazane Using Self‐Aligned Device Structure (2006)

Abstract We have fabricated organic thin‐film transistors that had a high field‐effect mobility of 0.39 cm 2 /Vs, in which both the semiconductor and the gate‐dielectric were solution‐processed using pentacene and polysilazane. These materials were also applied to a self‐aligned structure for the first time and exhibited good device performance with solution‐processed electrodes.
The Monospecificity of Novel Anti-HLA-E Monoclonal Antibodies Enables Reliable Immunodiagnosis, Immunomodulation of HLA-E, and Upregulation of CD8+ T Lymphocytes (2015)

In human cancers, over-expression of HLA-E is marked by gene expression. However, immunolocalization of HLA-E on tumor cells is impeded by the HLA-Ia reactivity of commercial anti-HLA-E monoclonal antibodies (MAbs). So there was a clear need to develop monospecific anti-HLA-E MAbs for reliable immunodiagnosis of HLA-E, particularly considering the prognostic relevance of HLA-E in human cancer. HLA-E overexpression is correlated with disease progression and poor survival of patients, both of which are attributed to the suppression of anti-tumor activity of cytotoxic T cells mediated by HLA-E. The suppression mechanism involves the binding of HLA-E-specific amino acids located on the α1 and α2 helices of HLA-E to the inhibitory receptors (CD94/NKG2a) on CD8+ T lymphocytes. An anti-HLA-E MAb that recognizes these HLA-E-specific sequences can not only be a monospecific MAb with potential for specific immunolocalization of HLA-E but can also block the sequences from interacting with the CD94/NKG2a receptors. We therefore developed several clones that secrete such HLA-E-specific MAbs; then we assessed the ability of the MAbs to bind to the amino acid sequences interacting with the CD94/NKG2a receptors by inhibiting them from binding to HLA-E with peptides that inhibit receptor binding. Elucidation of the immunomodulatory capabilities of these monospecific MAbs showed that they can induce proliferation of CD8+ T cells with or without co-stimulation. These novel MAbs can serve a dual role in combating cancer by blocking interaction of HLA-E with CD94/NKG2a and by promoting proliferation of both non-activated and activated CD8+ cytotoxic αβ T cells.
Statistical pattern analysis of partial discharge measurements for quality assessment of insulation systems in high voltage electrical machinery (2003)

In this paper, we present a new statistical analysis method of phase resolved partial discharge (PD) measurements for the quality assessment of the electrical insulation in high voltage machinery. The method is based on a supervised classification approach which utilizes histogram similarity analysis. The motivation for choosing histogram similarity analysis is twofold: first the phase resolved PD measurement itself is, in fact, a two dimensional histogram. Therefore a histogram matching based approach suits the very nature of the data. Second histogram similarity analysis combines the typical statistical parameters, such as mean, variance and higher order moments, used in the recognition of PD measurements in a statistically powerful and rigorous way. In our study, we utilize various histogram types and similarity analysis, including correlation, chi-square and Kolmogorov Smirnov tests. Our experimental study on the laboratory samples demonstrates that the method shows strong potential in defection and identification of insulation defects. The results from our study suggest that the proposed method provides a powerful, general and mathematically simple approach to the analysis of phase resolved PD measurements.
A Quick Test To Monitor the Delamination Propensity of Glass Containers (2014)

If pharmaceutical products do not match properly with the glass containers used, the creation of delaminated glass flakes can occur. This process is slow and time-dependent and mostly visible after month or years. With the Schott Delamination Quicktest a rapid method is developed that provides a fast measure for a rough indication of a higher risk for a delamination behavior. The application of the test gives quick evidence and can be used as a release criterion.
Polysilane photoresists for 193 and 248nm lithography. (1992)

A series of sensitizing additives for polysilane bilayer photoresists have been identified. These compounds effectively quench the fluorescence of aromatic polysilanes and greatly increase the rate of photooxidation and spectral bleaching in the solid state. Resists formulated with sensitizers such as phthalimidotriflate show increased photospeed in imaging experiments at both 248 and 193 nm. Characteristics of the resist reactions are described.
An Efficient Phase Domain Synchronous Machine Model With Constant Equivalent Admittance Matrix (2019)

In this paper, a new synchronous machine model is developed for electromagnetic transients program type simulations. The stator circuit is expressed in the abc phase domain. The machine model is represented as a Norton equivalent with a current source in parallel with a constant Norton admittance. The machine equations are reformulated so that the computational effort required for the modeling of the machine is reduced. Test studies demonstrate the accuracy of the proposed synchronous machine model and show that the proposed model is computationally more efficient than the existing constant conductance phase domain model and voltage-behind-reactance model.
FVCAG (2016)

Generation of accurate IP power models requires determination of correct simulation conditions for the different input pins of the IP. Determining such a set of inputs for individual IP blocks in a design is expensive in cost and time, and is also highly error prone. Additionally, it is desirable to identify IP instances in a design, where these simulation conditions are not met. These are relevant problems in the context of modern day microprocessor designs, which are designed using a very large number of IPs, either developed in-house or sourced from external vendors. In this paper, we examine these problems in an industrial context and introduce FVCAG, a framework for enabling efficient and accurate power modelling. FVCAG enables a more thorough IP power modelling than that can be accomplished using current state of the art techniques. Experimental evaluation of the proposed framework on the standard cell library and macros used in the design of an industry class high performance microprocessor design demonstrates the accuracy and efficiency of proposed framework.
Low-complexity 2D to 3D video conversion (2011)

3D film and 3D TV are becoming reality. More facilities and devices are now 3D capable. Compared to capture 3D video content directly, 2D to 3D video conversion is a low-cost, backward compatible alternate. There also exists a tremendous amount of monoscopic 2D video content that are of high interest to be displayed on 3D devices with noticeable immersiveness. 2D to 3D video conversion, therefore, has drawn lots of attention recently. In this paper, a low complexity 2D to 3D conversion algorithm is presented. The conversion generates stereo video pairs by 3D warping based on estimated per-pixel depth maps. The depth maps are estimated jointly by motion and color cues. Subjective tests show that the proposed algorithm achieves 3D perception with acceptable artifact.
Content and host-centric information dissemination in delay-tolerant smartphone MANETs: An architecture and demonstration (2012)

Usage of mobile commercial communication devices is continually increasing. However, these devices require a fixed infrastructure and do not function in mobile ad-hoc networks (MANETs) characterized by large delays or disruptions. Furthermore, these devices do not support the emerging communications needed when the location of information publishers and consumers is unknown (e.g., content centric communication models). To provide these capabilities, the paper presents an architecture that overlays content-centric and host-centric delay tolerant algorithms over a MANET. For validating the architecture, we provide a proof-of-concept implementation on Android phones as the illustrative mobile device and WiFi as the illustrative protocol, and we test the architecture in two field demonstrations.
Discovery of Double-Stranded Genomic DNA in Circulating Exosomes (2016)

It is becoming increasingly clear that small vesicles released from cells (extracellular vesicles [EVs]) represent a heterogeneous population implicated in cell-to-cell communication. The classifications and nomenclature of EVs are evolving as enrichment strategies and specific characteristics are being unraveled. At present, physical properties of EVs-namely, size, shape, and density-are often used to identify subpopulations of EVs. A distinct group of EVs, termed exosomes, largely defined by their small size (∼40-150 nm) and proposed subcellular origin, has been extensively studied in several aspects of cancer biology. Exosomes are implicated in modulating behavior of cancer cells as well as the immune and angiogenic responses in tumors, possibly contributing to cancer progression locally and systemically. Most intriguingly, the nucleic acid content of exosomes has been proposed to play a role in oncogenic transformation and transfer of cancer-specific genome to promote cancer pathogenesis. Here, we specifically focus on the discovery of exosomal DNA, studies related to the origin of genomic DNA in exosomes, and its utility in cancer diagnosis and disease monitoring.
Evidence for an Activation Domain at the Amino Terminus of Simian Immunodeficiency Virus Vpx (2009)

Vpx and Vpr are related lentiviral accessory proteins that enhance virus replication in macrophages and dendritic cells. Both proteins are packaged into virions and mediate their effects in the target cell through an interaction with an E3 ubiquitin ligase that contains DCAF1 and DDB1. When introduced into primary macrophages and dendritic cells in viruslike particles, Vpx can enhance the efficiency of a subsequent infection. Here, we confirm the ability of Vpx to enhance simian immunodeficiency virus (SIV) and human immunodeficiency virus type 1 (HIV-1) infection of macrophages up to 100-fold by using single-cycle reporter viruses and by pretreatment of the cells with Vpx-containing viruslike particles. Vpx was also active in differentiated THP-1 cells but not in other cell lines. Induction of an antiviral state in macrophages with type I interferon significantly magnified the effect of Vpx on HIV-1 infection, suggesting that Vpx helps the virus to overcome an inducible intracellular restriction. Quantitative PCR quantitation of SIV and HIV-1 reverse transcripts in newly infected macrophages showed that the block was at an early step in reverse transcription. In spite of its structural similarity, Vpr was inactive. This difference allowed us to map the functional domains of Vpx with a panel of Vpr/Vpx chimeras. Analysis of the chimeras demonstrated that the amino-terminal domain of Vpx is important for the enhancement of infection. Fine mapping of the region indicated that amino acids at positions 9, 12, and 15 to 17 were required. Although the mutants failed to enhance infection, they retained their ability to interact with DCAF1. These findings suggest that the Vpx amino terminus contains an activation domain that serves as the binding site for a cellular restriction factor.
<title>Interrogation and multiplexing techniques for fiber Bragg grating strain sensors</title> (1993)

Techniques for the detection of wavelength shifts of fiber Bragg grating sensors based on the use of a tunable bandpass filter, interferometric detection, and active laser configurations are described. The methods used to multiplex several sensors along a single fiber using these approaches are also discussed, and results presented.