gebhart/patrepeval huggingface dataset:
Modulation of cis-platinum resistance in Friend erythroleukemia cells by c-myc. (1991)

The molecular genetic basis for cancer cell resistance to the important family of platinum coordination complex drugs is poorly understood. The observation that malignant cells commonly become resistant to therapy, while normal cells rarely do, suggests that certain molecular processes involved in malignancy, e.g., oncogene activation, might also play a role in drug resistance. Since aberrant expression and amplification of various myc oncogenes have been implicated in the prognosis of human cancers, the poor therapeutic response of some of these tumors might be explained if high levels of myc gene products rendered cells more refractory to therapy. We tested this hypothesis by determining the effect of varying the level of c-myc expression on resistance to cis-platinum and ionizing radiation in Friend murine erythroleukemia cells expressing varying levels of c-myc gene product. We found that a) the degree of cis-platinum resistance correlated directly with the level of c-myc expression, b) glucocorticoid induction of murine mammary tumor virus promoter-driven c-myc sequences significantly increased cis-platinum resistance, c) restoring c-myc transcript levels to normal restored the original cis-platinum sensitivity at a rate which paralleled that of induced c-myc transcript depletion, and d) c-myc transcript level had no effect on ionizing radiation response. These findings suggest that c-myc levels may influence therapeutic success in some tumors and may regulate specific processes by which cells cope with DNA damage caused by DNA cross-linking agents such as the platinum analogues, but not ionizing radiation.
Space Intersection From Ers‐1 Synthetic Aperture Radar Images (1996)

derivation of spatial data from European Remote Sensing Satellite (ERS‐1) Synthetic Aperture Radar (SAR) imagery is briefly described in this paper. A rigorous algorithm for space intersection applied in different geometric configurations and combinations, including the “same‐side” image pair and the “opposite‐side” pair, is illustrated. A simple error model is proposed by the authors to validate the error from different radargrammetric configurations. The experimental results show the possibilities and the limitations of obtaining three dimensional information from spaceborne SAR imagery with or without using ground control points. At the present stage, as a supplementary role in space cartography, the ERS‐1 SAR imagery not only provides a new way to see the earth through clouds but also makes available an alternative tool to map the planet.
Mobile Cloud for Assistive Healthcare (MoCAsH) (2010)

Deploying state-of-the-art technologies is vital and inevitable in assistive healthcare to cope with emerging services such as remote monitoring, collaborative consultation, and electronic health record. Grid computing has succeeded somewhat in enabling the sharing of resources across organizations but has not been deployed widely due to its complex implementation and interface. Cloud computing overcomes this aspect by allowing simple and easy user access, coping with users' dynamic and elastic demands, providing metered usage for its resources and hence is increasingly being adopted by individual users as well as enterprise users. The Cloud may just be the right technology for healthcare infrastructure. However, several serious issues concerning security, data protection and ownership, quality of services, and mobility need to be resolved before Cloud computing can be widely adopted. This paper proposes Mobile Cloud for Assistive Healthcare (MoCAsH) as an infrastructure for assistive healthcare. Besides inheriting the advantages of Cloud computing, MoCAsH embraces important concepts of mobile sensing, active sensor records, and collaborative planning by deploying intelligent mobile agents, context-aware middleware, and collaborative protocol for efficient resource sharing and planning. MoCAsH addresses security and privacy issues by deploying selective and federated P2P Cloud to protect data, preserve data ownership and strengthen aspects of security. It also addresses various quality-of-service issues concerning critical responses and energy consumption.
A Comparison of Reinforcement Learning Techniques for Fuzzy Cloud Auto-Scaling (2017)

A goal of cloud service management is to design self-adaptable auto-scaler to react to workload fluctuations and changing the resources assigned. The key problem is how and when to add/remove resources in order to meet agreed service-level agreements. Reducing application cost and guaranteeing service-level agreements (SLAs) are two critical factors of dynamic controller design. In this paper, we compare two dynamic learning strategies based on a fuzzy logic system, which learns and modifies fuzzy scaling rules at runtime. A self-adaptive fuzzy logic controller is combined with two reinforcement learning (RL) approaches: (i) Fuzzy SARSA learning FSL and (ii) Fuzzy Q-learning FQL. As an off-policy approach, Q-learning learns independent of the policy currently followed, whereas SARSA as an on-policy always incorporates the actual agent's behavior and leads to faster learning. Both approaches are implemented and compared in their advantages and disadvantages, here in the OpenStack cloud platform. We demonstrate that both auto-scaling approaches can handle various load traffic situations, sudden and periodic, and delivering resources on demand while reducing operating costs and preventing SLA violations. The experimental results demonstrate that FSL and FQL have acceptable performance in terms of adjusted number of virtual machine targeted to optimize SLA compliance and response time.
Recovery of motor function after stroke. (1988)

The natural history of recovery of motor function after stroke is described using data from a 1-year community-based study in Auckland, New Zealand. Of 680 patients, 88% presented with a hemiparesis; the proportion of survivors with a persisting deficit declined to 71% at 1 month and 62% at 6 months after the onset of the stroke. At onset, there were equal proportions of people with mild, moderate, and severe motor deficits, but the majority (76%) of those who survived 6 months had either no or only a mild deficit. Recovery of motor function was associated with the stroke severity but not with age or sex; patients with a mild motor deficit at onset were 10 times more likely to recover their motor function than those with a severe stroke. Our results confirm the reasonably optimistic outcome for survivors of stroke and further suggest that recovery of motor function is confined to patients whose motor deficit at onset is either mild or moderate.
Epidemic spreading in a hierarchical social network (2004)

A model of epidemic spreading in a population with a hierarchical structure of interpersonal interactions is described and investigated numerically. The structure of interpersonal connections is based on a scale-free network. Spatial localization of individuals belonging to different social groups, and the mobility of a contemporary community, as well as the effectiveness of different interpersonal interactions, are taken into account. Typical relations characterizing the spreading process, like a range of epidemic and epidemic curves, are discussed. The influence of preventive vaccinations on the spreading process is investigated. The critical value of preventively vaccinated individuals that is sufficient for the suppression of an epidemic is calculated. Our results are compared with solutions of the master equation for the spreading process and good agreement of the character of this process is found.
Free radicals and brain damage. (1989)

Although free radicals have been suggested to contribute to ischemic brain damage for more than 10 years, it is not until quite recently that convincing evidence has been presented for their involvement in both sustained and transient ischemia. The hypothesis is examined against current knowledge of free radical chemistry, as it applies to biological systems, and of cellular iron metabolism. It is emphasized that those advents have changed our outlook on free radical-induced tissue damage. First, it has been realized that damage to DNA and proteins may be an earlier event than lipid peroxidation, perhaps also a more important one. Second, evidence now exists that the triggering event in free radical-induced damage is a disturbance of cellular iron metabolism, notably delocalization of protein-bound iron, and its chelation by compounds that trigger site-specific free radical damage. Third, methods have been developed that allow the demonstration of partially induced oxygen species in tissues, and scavengers have become available that can curb free radical reactions. As a result of these events, it has been possible to demonstrate formation of free radicals in oxygen toxicity, trauma, and ischemia, and their participation in the cell damage that is incurred in these conditions, particularly in causing vascular pathology and edema. It is suggested that in ischemia, free radical damage becomes pathogenetically important when the ischemia is of long duration, when conditions favor continued delivery of some oxygen to the ischemic tissue, and particularly when such partially oxygen-deprived tissue is reoxygenated.
Growing closer on facebook (2014)

Scientists debate whether people grow closer to their friends through social networking sites like Facebook, whether those sites displace more meaningful interaction, or whether they simply reflect existing ties. Combining server log analysis and longitudinal surveys of 3,649 Facebook users reporting on relationships with 26,134 friends, we find that communication on the site is associated with changes in reported relationship closeness, over and above effects attributable to their face-to-face, phone, and email contact. Tie strength increases with both one-on-one communication, such as posts, comments, and messages, and through reading friends' broadcasted content, such as status updates and photos. The effect is greater for composed pieces, such as comments, posts, and messages than for 'one-click' actions such as 'likes.' Facebook has a greater impact on non-family relationships and ties who do not frequently communicate via other channels.
A randomized trial to evaluate the risk of gastrointestinal disease due to consumption of drinking water meeting current microbiological standards. (1991)

It is estimated that 35% of the reported GI illnesses among the tapwater drinkers were water-related and preventable. Our results raise questions about the adequacy of current standards of drinking water quality to prevent water-borne endemic gastrointestinal illness.
CRISPR-MVLST subtyping of Salmonella enterica subsp. entericaserovars Typhimurium and Heidelberg and application in identifying outbreak isolates (2013)

For S. Heidelberg, our data shows that combined typing by both CRISPR-MVLST and PFGE provided a discriminatory power of 0.9213. Importantly, CRISPR-MVLST was able to separate common PFGE patterns such as JF6X01.0022 into distinct STs, thus providing significantly greater discriminatory power. Conversely, we show that subtyping by either CRISPR-MVLST or PFGE independently provides a sufficient discriminatory power (0.9345 and 0.9456, respectively) for S. Typhimurium. Additionally, using isolates from two S. Typhimurium outbreaks, we demonstrate that CRISPR-MVLST provides excellent epidemiologic concordance.
Temperature dependence of Brillouin frequency, power, and bandwidth in panda, bow-tie, and tiger polarization-maintaining fibers (2004)

We report a study of the temperature dependence of the Brillouin gain and loss for three different kinds of commercial polarization-maintaining fibers for the first time to our knowledge. The Brillouin frequency differences between the fast and slow axes are independent of the temperature, varying between 2.9 and 4.3 MHz. Using 2-ns pulses (equivalent to a spatial resolution of 20 cm), we find that the temperature coefficients for the relative Brillouin power at a wavelength of 1310 nm are 0.26%/degrees C (panda fiber), 0.23%/degrees C (bow-tie fiber), and 0.04%/degrees C (tiger fiber); the temperature coefficients for the Brillouin frequency are 1.37 MHz/degrees C (panda), 1.66 MHz/degrees C (tiger), and 2.30 MHz/degrees C (bow-tie). The temperature coefficients for the Brillouin gain bandwidth are 0.15 MHz/degrees C (panda), 0.20 MHz/degrees C (bow-tie), and 0.22 MHz/degrees C (tiger).
Silicon carbide, a semiconductor for space power electronics (1991)

After many years of promise as a high temperature semiconductor, silicon carbide (SiC) is finally emerging as a useful electronic material. Recent significant progress that has led to this emergence has been in the areas of crystal growth and device fabrication technology. High quality single‐crystal SiC wafers, up to 25 mm in diameter, can now be produced routinely from boules grown by a high temperature (2700 K) sublimation process. Device fabrication processes, including chemical vapor deposition (CVD), in situ doping during CVD, reactive ion etching, oxidation, metallization, etc. have been used to fabricate p–n junction diodes and MOSFETs. The diode was operated to 870 K and the MOSFET to 770 K.
Effects of coronary artery disease on expression and microvascular response to VEGF (1998)

The effects of coronary artery disease (CAD) on human coronary microvascular responses to vascular endothelial growth factor (VEGF) and the alterations of the myocardial expressions of VEGF and its flk-1 and flt-1 receptors were examined in 48 patients. Microvascular studies were performed in vitro with video microscopy. The expressions of VEGF and its receptors were examined using Northern analysis of total mRNA, and the expressions of constitutive nitric oxide synthase (cNOS) and inducible nitric oxide synthase (iNOS) were examined by RT-PCR. VEGF and hepatocyte growth factor (HGF) caused potent relaxations of microvessels. These responses were reduced in the presence of NG-nitro-L-arginine and the tyrosine kinase inhibitor genistein or in microvessels from patients with CAD. Relaxations to substance P and sodium nitroprusside were similar in both groups. The substance P response was abolished in the presence of NG-nitro-L-arginine. The expression of VEGF and its receptors and the expression of cNOS and iNOS were not altered in patients with CAD. In conclusion, VEGF and HGF elicit the release of nitric oxide through activation of tyrosine kinase receptors. CAD is associated with reduced vascular responses to both VEGF and HGF; this is not likely due to a reduced expression of VEGF or flt-1 or flk-1 receptors and not due to a generalized endothelium dysfunction despite the presence of mild hypercholesterolemia in these patients with CAD. These findings may have important implications regarding the efficacy of endogenous and exogenous VEGF in patients with risk factor for CAD.
The selective release of phospholipase A2 by resident mouse peritoneal macrophages (1981)

Resident mouse peritoneal macrophages have three phospholipase activities: a phospholipase A2 active at pH 4.5, a Ca2+-dependent phospholipase A2 active at pH 8.5 and a phosphatidylinositol-specific phospholipase C activity. When macrophages are exposed to zymosan in culture, the cellular activity of pH-4.5 phospholipase A2 is diminished in a manner dependent on zymosan concentration and time of exposure, whereas the cellular activities of pH-8.5 phospholipase A2 and phospholipase C remain unchanged. The depletion of pH-4.5 phospholipase A2 activity from the cell is paralleled by a quantitative recovery of this activity in the culture medium in a manner similar to the cellular depletion and extracellular recovery of two lysosomal enzymes. This release is specifically elicited by an inflammatory substance such as zymosan, since macrophages incubated with 6 micrometer latex spheres retain pH-4.5 phospholipase A2 activity and lysosomal enzyme activities intracellularly.
Molecular Basis of Sulfonylurea Herbicide Inhibition of Acetohydroxyacid Synthase (2003)

Acetohydroxyacid synthase (AHAS) (acetolactate synthase, EC ) catalyzes the first step in branched-chain amino acid biosynthesis and is the target for sulfonylurea and imidazolinone herbicides. These compounds are potent and selective inhibitors, but their binding site on AHAS has not been elucidated. Here we report the 2.8 A resolution crystal structure of yeast AHAS in complex with a sulfonylurea herbicide, chlorimuron ethyl. The inhibitor, which has a K(i) of 3.3 nm, blocks access to the active site and contacts multiple residues where mutation results in herbicide resistance. The structure provides a starting point for the rational design of further herbicidal compounds.
Airborne Integrated Mapping System: Positioning Component (1997)

The Center for Mapping at The Ohio State University is
currently developing a fully digital Airborne Integrated
Mapping System (AIMS) for large-scale mapping and
other precise positioning applications. AIMS will be
installed in an aerial platform and will incorporate state-
of-the-art positioning (differential GPS integrated with
INS) and imaging (CCD, SAR) technologies. The
project’s goal is to develop a low-cost hardware
prototype that acquires position and orientation of an
aerial platform with accuracy of 4-7 cm and below 10
arcsec, respectively, over long baselines, and performs
essential processing of digital imagery in real-time and in
post-processing mode. The potential applications include
large-scale topographic mapping, military reconnaissance,
real-time target tracking, corridor surveys of the
transportation infrastructures, gas, electric and
telecommunication networks, drug interdiction efforts,
and emergency response deployment. A tightly integrated
Global Positioning System and high-accuracy Inertial
Navigation System (GPS/INS) provide orientation and
position of the aerial platform. A single Kalman filter
optimally estimates position, velocity, and attitude
parameters, as well as errors in GPS and INS
measurements. This paper presents the AIMS architecture
and implementation, with special emphasis on the
positioning component of the system, together with
airborne test results.
Alterations in dendritic cell phenotype and function associated with immunoenhancing effects of a subcutaneously administered cyclophosphamide derivative. (1991)

A single systemic dose of cyclophosphamide (CY) has been shown to enhance cellular immunity in a variety of antigen models. The immunoenhancing effects of CY have been attributed to its ability to selectively abrogate suppressor cell function. Previous studies from our group have demonstrated that local administration of distinct cytostatic drugs at the sensitization site can induce a similar enhancement of delayed-type hypersensitivity as systemic CY, with the obvious advantage of avoiding systemic side-effects. In the present study we investigated the effects of local administration of an optimally immunopotentiating dose of the active CY-derivative Z 7557 and, in selected experiments, of etoposide (VP-16) and systemic CY on mononuclear cells in draining lymph nodes. Whereas CY caused a long-lasting and marked depletion of B-cell areas, locally administered Z 7557 and VP-16 relatively spared B cells and even induced an increase in B- and T-cell numbers in (keyhole limpet haemocyanin-) sensitized mice. At Day 4 the CD4/CD8 ratio was slightly reduced in drug-treated mice. Interestingly, drug treatment reduced the proportion of interdigitating cells staining with the monoclonal antibodies NLDC-145 and MIDC-8. Upon isolation, dendritic cells (DC) from sensitized, Z 7557-treated mice showed longer dendritic protrusions and an enhanced accessory cell function compared to DC from saline-treated controls. These findings suggest that immunoenhancing effects of cytostatic drugs may occur via an effect on DC.
Role of ceramide in diabetes mellitus: evidence and mechanisms (2013)

Diabetes mellitus is a metabolic disease with multiple complications that causes serious diseases over the years. The condition leads to severe economic consequences and is reaching pandemic level globally. Much research is being carried out to address this disease and its underlying molecular mechanism. This review focuses on the diverse role and mechanism of ceramide, a prime sphingolipid signaling molecule, in the pathogenesis of type 1 and type 2 diabetes and its complications. Studies using cultured cells, animal models, and human subjects demonstrate that ceramide is a key player in the induction of β-cell apoptosis, insulin resistance, and reduction of insulin gene expression. Ceramide induces β-cell apoptosis by multiple mechanisms namely; activation of extrinsic apoptotic pathway, increasing cytochrome c release, free radical generation, induction of endoplasmic reticulum stress and inhibition of Akt. Ceramide also modulates many of the insulin signaling intermediates such as insulin receptor substrate, Akt, Glut-4, and it causes insulin resistance. Ceramide reduces the synthesis of insulin hormone by attenuation of insulin gene expression. Better understanding of this area will increase our understanding of the contribution of ceramide to the pathogenesis of diabetes, and further help in identifying potential therapeutic targets for the management of diabetes mellitus and its complications.
Cognitive Deficits Associated with Nav1.1 Alterations: Involvement of Neuronal Firing Dynamics and Oscillations (2016)

Brain oscillations play a critical role in information processing and may, therefore, be essential to uncovering the mechanisms of cognitive impairment in neurological disease. In Dravet syndrome (DS), a mutation in SCN1A, coding for the voltage-gated sodium channel Nav1.1, is associated with severe cognitive impairment and seizures. While seizure frequency and severity do not correlate with the extent of impairment, the slowing of brain rhythms may be involved. Here we investigate the role of Nav1.1 on brain rhythms and cognition using RNA interference. We demonstrate that knockdown of Nav1.1 impairs fast- and burst-firing properties of neurons in the medial septum in vivo. The proportion of neurons that fired phase-locked to hippocampal theta oscillations was reduced, and medial septal regulation of theta rhythm was disrupted. During a working memory task, this deficit was characterized by a decrease in theta frequency and was negatively correlated with performance. These findings suggest a fundamental role for Nav1.1 in facilitating fast-firing properties in neurons, highlight the importance of precise temporal control of theta frequency for working memory, and imply that Nav1.1 deficits may disrupt information processing in DS via a dysregulation of brain rhythms.
Hereditary diseases of coenzyme A thioester metabolism (2019)

Coenzyme A (CoA) thioesters (acyl-CoAs) are essential intermediates of metabolism. Inborn errors of acyl-CoA metabolism include a large fraction of the classical organic acidemias. These conditions can involve liver, muscle, heart and brain, and can be fatal. These conditions are increasingly detected by newborn screening. There is a renewed interest in CoA metabolism and in developing effective new treatments. Here, we review theories of the pathophysiology in relation to mitochondrial CoA sequestration, toxicity and redistribution (CASTOR).