gebhart/patrepeval huggingface dataset:
Overexpression of a chromatin architecture‐controlling AT‐hook protein extends leaf longevity and increases the post‐harvest storage life of plants (2007)

Leaf senescence is the final stage of leaf development and is finely regulated via a complex genetic regulatory network incorporating both developmental and environmental factors. In an effort to identify negative regulators of leaf senescence, we screened activation-tagged Arabidopsis lines for mutants that exhibit a delayed leaf senescence phenotype. One of the mutants (ore7-1D) showed a highly significant delay of leaf senescence in the heterozygous state, leading to at least a twofold increase in leaf longevity. The activated gene (ORE7/ESC) encoded a protein with an AT-hook DNA-binding motif; such proteins are known to co-regulate transcription of genes through modification of chromatin architecture. We showed that ORE7/ESC, in addition to binding to a plant AT-rich DNA fragment, could also modify the chromatin architecture, as illustrated by an altered distribution of a histone-GFP fusion protein in the nucleus of the mutant. Globally altered gene expression, shown by microarray analysis, also indicated that activation of ORE7/ESC results in a younger condition in the mutant leaves. We propose that ectopically expressed ORE7/ESC is negatively regulating leaf senescence and suggest that the resulting chromatin alteration may have a role in controlling leaf longevity. Interestingly, activation of ORE7/ESC also led to a highly extended post-harvest storage life.
Design and Synthesis of a Chitodisaccharide-Based Affinity Resin for Chitosanases Purification (2019)

Chitooligosaccharides (CHOS) have gained increasing attention because of their important biological activities. Enhancing the efficiency of CHOS production essentially requires screening of novel chitosanase with unique characteristics. Therefore, a rapid and efficient one-step affinity purification procedure plays important roles in screening native chitosanases. In this study, we report the design and synthesis of affinity resin for efficient purification of native chitosanases without any tags, using chitodisaccharides (CHDS) as an affinity ligand, to couple with Sepharose 6B via a spacer, cyanuric chloride. Based on the CHDS-modified affinity resin, a one-step affinity purification method was developed and optimized, and then applied to purify three typical glycoside hydrolase (GH) families: 46, 75, and 80 chitosanase. The three purified chitosanases were homogeneous with purities of greater than 95% and bioactivity recovery of more than 40%. Moreover, we also developed a rapid and efficient affinity purification procedure, in which tag-free chitosanase could be directly purified from supernatant of bacterial culture. The purified chitosanases samples using such a procedure had apparent homogeneity, with more than 90% purity and 10⁻50% yield. The novel purification methods established in this work can be applied to purify native chitosanases in various scales, such as laboratory and industrial scales.
Nonlinear Effects of RF-Photonic Cancellation in IBFD Systems (2020)

Future wireless systems will allow users to achieve high data rates by accessing wide bandwidths using carrier aggregation in combination with high individual channel bandwidths. The wideband nature of these systems makes the incorporation of In-Band Full-Duplex (IBFD) technology more challenging because the percentage bandwidths increase and multipath self-interference becomes more frequency-selective. These issues can be tackled by introducing RF-photonic adaptive cancellers into IBFD systems to provide wideband cancellation in the RF domain, but can only be accomplished if the nonlinear effects of these devices are appropriately considered. This paper presents a theoretical nonlinear framework for such cancellers, and uniquely discusses their influence within IBFD systems using measured results of a prototype canceller.
Space-time coding and signal processing for high data rate wireless communications (2001)

The information capacity of wireless communication systems can be increased dramatically by employingmultiple transmit and receive antennas [?, ?]. An effective approach to increasing data rate over wireless channels is to employ coding techniques appropriate to multiple transmit antennas, that is space-time coding. Space-time codes introduce temporal and spatial correlation into signals transmitted from different antennas, in order to provide diversity at the receiver, and coding gain over an uncoded system. The spatial-temporal structure of these codes can be exploited to further increase the capacity of wireless systems with a relatively simple receiver structure. This chapter provides an overview of space-time coding techniques and the associated signal processing framework.
Transition-metal-promoted reactions of boron hydrides. 12. Syntheses, polymerizations, and ceramic conversion reactions of B-alkenylborazines (1989)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTransition-metal-promoted reactions of boron hydrides. 12. Syntheses, polymerizations, and ceramic conversion reactions of B-alkenylborazinesAnne T. Llynch and Larry G. SneddonCite this: J. Am. Chem. Soc. 1989, 111, 16, 6201–6209Publication Date (Print):August 1, 1989Publication History Published online1 May 2002Published inissue 1 August 1989https://pubs.acs.org/doi/10.1021/ja00198a034https://doi.org/10.1021/ja00198a034research-articleACS PublicationsRequest reuse permissionsArticle Views375Altmetric-Citations47LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Detection and quantification of valve stiction (2006)

Stiction is a common problem in spring-diaphragm type valves, which are widely used in the process industry. Recently, there have been many attempts to understand, define, model and detect stiction in control valves. Most of the current methods cannot simultaneously detect and quantify stiction. This paper discusses the definition and modelling of stiction. It describes two methods to detect and quantify valve stiction using routine operating data. The methods are completely data-based. No additional excitation or experimentation of the plant is required. The methods have been applied to many industrial data sets and their wide applicability and practicality as useful diagnostic aids in control-loop performance monitoring has been demonstrated
Optimize hardware with fixed-point variable length phase factors (2012)

Fast Fourier Transforms (FFTs) are highly parallel in nature and consist of simple addition, subtraction, and complex rotation operators with phase factors (a.k.a. twiddle factors). With the advent of FPGAs and other reconfigurable seas-of-logic, it is now possible to construct a fully parallel FFT structure where the phase factors are now constants and good targets for hardware optimization. By varying the fixed-point length of the phase factors using phase angle error percentage as a control for the variable length phase factor quantizer, the number of shifted adders required to implement the complex rotation operators can be reduced. Performance comparisons of fixed length and variable length phase factors, along with two quantizer rounding modes, are investigated.
Dynamic synthesis for relaxed memory models (2012)

Modern architectures implement relaxed memory models which may reorder memory operations or execute them non-atomically. Special instructions called memory fences are provided, allowing control of this behavior.
Hoard: A Distributed Data Caching System to Accelerate Deep Learning Training on the Cloud (2018)

Deep Learning system architects strive to design a balanced system where the computational accelerator -- FPGA, GPU, etc, is not starved for data. Feeding training data fast enough to effectively keep the accelerator utilization high is difficult when utilizing dedicated hardware like GPUs. As accelerators are getting faster, the storage media \& data buses feeding the data have not kept pace and the ever increasing size of training data further compounds the problem. We describe the design and implementation of a distributed caching system called Hoard that stripes the data across fast local disks of multiple GPU nodes using a distributed file system that efficiently feeds the data to ensure minimal degradation in GPU utilization due to I/O starvation. Hoard can cache the data from a central storage system before the start of the job or during the initial execution of the job and feeds the cached data for subsequent epochs of the same job and for different invocations of the jobs that share the same data requirements, e.g. hyper-parameter tuning. Hoard exposes a POSIX file system interface so the existing deep learning frameworks can take advantage of the cache without any modifications. We show that Hoard, using two NVMe disks per node and a distributed file system for caching, achieves a 2.1x speed-up over a 10Gb/s NFS central storage system on a 16 GPU (4 nodes, 4 GPUs per node) cluster for a challenging AlexNet ImageNet image classification benchmark with 150GB of input dataset. As a result of the caching, Hoard eliminates the I/O bottlenecks introduced by the shared storage and increases the utilization of the system by 2x compared to using the shared storage without the cache.
Introduction of an 8-Aminooctanoic Acid Linker Enhances Uptake of <sup>99m</sup>Tc-Labeled Lactam Bridge–Cyclized α-MSH Peptide in Melanoma (2014)

High melanoma uptake and fast urinary clearance of (99m)Tc(EDDA)-HYNIC-AocNle-CycMSHhex highlighted its potential for metastatic melanoma detection in the future.
Enabling drug discovery for the PARP protein family through the detection of mono-ADP-ribosylation (2019)

Poly-ADP-ribose polymerases (PARPs) are a family of enzymes responsible for transferring individual or chains of ADP-ribose subunits to substrate targets as a type of post-translational modification. PARPs regulate a wide variety of important cellular processes, ranging from DNA damage repair to antiviral response. However, most research to date has focused primarily on the polyPARPs, which catalyze the formation of ADP-ribose polymer chains, while the monoPARPs, which transfer individual ADP-ribose monomers, have not been studied as thoroughly. This is partially due to the lack of robust assays to measure mono-ADP-ribosylation in the cell. In this study, the recently developed MAR/PAR antibody has been shown to detect mono-ADP-ribosylation in cells, enabling the field to investigate the function and therapeutic potential of monoPARPs. In this study, the antibody was used in conjunction with engineered cell lines that overexpress various PARPs to establish a panel of assays to evaluate the potencies of literature-reported PARP inhibitors. These assays should be generally applicable to other PARP family members for future compound screening efforts. A convenient and generalizable workflow to identify and validate PARP substrates has been established. As an initial demonstration, aryl hydrocarbon receptor was verified as a direct PARP7 substrate and other novel substrates for this enzyme were also identified and validated. This workflow takes advantage of commercially available detection reagents and conventional mass spectrometry instrumentation and methods. Ultimately, these assays and methods will help drive research in the PARP field and benefit future therapeutics development.
Tumor Cell-Platelet Aggregation: Induced by Cathepsin B-Like Proteinase and Inhibited by Prostacyclin (1982)

The ability of tumor cells to metastasize may be related to their ability to promote aggregation of host platelets. The use of inhibitors of cysteine proteinases resulted in parallel inhibition of B16 amelanotic melanoma-induced platelet aggregation and of a cathepsin B activity. The antimetastatic agent prostacyclin inhibited platelet aggregation induced by the tumor cells and by papain, a cathepsin B-mimicking agent.
Low-voltage CMOS circuits for analog iterative decoders (2006)

Iterative decoders, including Turbo decoders, provide near-optimal error protection for various communication channels and storage media. CMOS analog implementations of these decoders offer dramatic savings in complexity and power consumption, compared to digital architectures. Conventional CMOS analog decoders must have supply voltage greater than 1 V. A new low-voltage architecture is proposed which reduces the required supply voltage by at least 0.4 V. It is shown that the low-voltage architecture can be used to implement the general sum-product algorithm. The low-voltage analog architecture is then useful for implementing Turbo and low-density parity check decoders. The low-voltage architecture introduces new requirements for signal normalization, which are discussed. Measured results for two fabricated low-voltage analog decoders are also presented.
TNFRSF25 promotes IL-17 and TL1A expression in murine CD4+ T cells (96.10) (2009)

Abstract TNFRSF25 or Death Receptor 3 (TNFR25; DR3) is a member of the TNF-receptor family with TL1A (TNFSF15) as its ligand. TNFR25 is expressed constitutively on murine CD4+ and CD8+ T cells at low levels and up-regulated after T cell activation. IL-17 is a pleiotropic cytokine associated with inflammation and autoimmunity. Using purified CD4+ T cells from transgenic mice over-expressing A. a full length (FL) TNFR25, B. a natural occurring splice variant, lacking exon 5 and 6 (Δ5,6), C. a dominant negative (DN) TNFR25, lacking the intracellular domain, and D. TL1A knockout mice, we investigated the role of TNFR25 signals in IL-17 expression. CD4+ T cells from full length and, to a lesser extend, those from Δ5,6 transgenic mice showed a significant increase in IL-17 secretion compared to wild type (wt) CD4+ T cells after primary and secondary stimulation under ThN and Th17 conditions in vitro. IL-17 levels in DN and TL1A-/- CD4+ T cells were comparable to wt. Cytokine levels of IFN-γ were comparable between wild type and transgenic/knockout mice, while Th2 cytokines were also increased in FL and Δ5,6 mice, as reported previously. Importantly, we detected highly elevated TL1A mRNA levels in un-stimulated full lenght CD4+ T cells. Our data indicate that TNFR25 signals cause increased secretion of both Th2 and Th17 cytokines and elevate TL1A expression by CD4+ T cells. TNFR25 signals thus support inflammatory and allergic immune responses.
<i>PKHD1</i>mutations in autosomal recessive polycystic kidney disease (ARPKD) (2004)

Autosomal recessive polycystic kidney disease (ARPKD) is an important cause of childhood renal- and liver-related morbidity and mortality. The clinical spectrum is widely variable. About 30 to 50% of affected individuals die in the neonatal period, while others survive into adulthood. ARPKD is caused by mutations in the PKHD1 (polycystic kidney and hepatic disease 1) gene on chromosome 6p12, which is among the largest human genes, with a minimum of 86 exons assembled into a variety of alternatively spliced transcripts. The longest continuous open reading frame is predicted to yield a 4,074-aa (447-kDa) multidomain integral membrane protein (fibrocystin/polyductin) of unknown function. This update compiles all known PKHD1 mutations and polymorphisms/sequence variants. Mutations were found to be scattered throughout the gene without evidence of clustering at specific sites. Most PKHD1 mutations are unique to single families ("private mutations") hampering genotype-phenotype correlations. Correlations have been drawn for the type of mutation rather than for the site of individual mutations. All patients carrying two truncating mutations displayed a severe phenotype with perinatal or neonatal demise, while patients surviving the neonatal period bear at least one missense mutation. However, some missense changes are obviously as devastating as truncating mutations. The present article intends 1) to provide an overview of PKHD1 mutations and polymorphisms/sequence variants identified so far, 2) to discuss potential genotype-phenotype correlations, and 3) to review them in the context of their clinical implications. A constantly updated list of mutations is available online (www.humgen.rwth-aachen.de) and investigators are invited to submit their novel data to this PKHD1 mutation database.
Bifunctional T-Cell-Derived Cytokines for the Diagnosis of Tuberculosis and Treatment Monitoring (2014)

The addition of antigen-specific IL-2 production to IFN-γ responses by EliSpot in IFN-γ release assays increases diagnostic sensitivity for active tuberculosis.
<title>Relaxor-based single-crystal materials for ultrasonic transducer applications</title> (1997)

Relaxor ferroelectric single crystals of Pb(Zn<SUB>1/3</SUB>Nb<SUB>2/3</SUB>)O<SUB>3</SUB> (PZN), Pb(Mg<SUB>1/3</SUB>Nb<SUB>2/3</SUB>)O<SUB>3</SUB> (PMN) and their solid solutions with normal ferroelectric PbTiO<SUB>3</SUB> (PT) were investigated for ultrasonic transducer applications. Crystals offer adjustable properties not only by compositional tailoring but also by domain state engineering associated with different crystallographic orientation, which is not achievable in polycrystalline materials. Longitudinal coupling coefficients (k<SUB>33</SUB>) as high as 94% and dielectric constants (K<SUB>3</SUB><SUP>T</SUP>) in the range of 3500 - 6000 were achieved with low dielectric loss (less than 1%) using <001> oriented rhombohedral crystals of (1-x)PZN-xPT and (1-y)PMN-yPT, where x less than 0.09 and y less than 0.35. Dicing direction as well as poling direction were critical for high coupling under laterally clamped condition. Dicing parallel to the (001) yields 90% of laterally clamped coupling (k<SUB>bar</SUB>) out of 94% longitudinal coupling (k<SUB>33</SUB>) for PZN-8%PT. On the other hand, samples diced parallel to (110) exhibited no dominant mode present. Thickness coupling (k<SUB>T</SUB>) as high as 64% and low dielectric constant (K<SUB>3</SUB><SUP>T</SUP>) less than 600 with low loss (less than 1%) could be achieved using tetragonal crystals of (1-x)PZN-xPT and (1-y)PMN-yPT, where x greater than 0.1 and y greater than 0.4. The performance gains associated with these ultra-high coupling coefficients and range of dielectric constants are evident in relation to broader bandwidth and electrical impedance matching. Specifically, rhombohedral crystals offer the possibility of extremely broad bandwidth devices for transducer arrays and tetragonal crystals for single element transducers. Transducer simulation was performed using the KLM model. The pulse/echo response simulated a 124% bandwidth subdiced array element with a center frequency of 10 MHz. An optimized array design of the same geometry constructed of PZT 5H displays an 87% bandwidth.
Multiplexed coherent optical processor for calculating generalized moments (1982)

A coherent optical processor capable of calculating generalized moments of a two-dimensional pattern is described. A spatial-frequency multiplexing scheme is used to provide for parallel computation of multiple moments. The use of a computer-generated holographic mask permits complete flexibility in choosing moment-generating functions; e.g., the functions could be complex or have a predetermined weighting function. Experimentally, calculation of five geometric moments (corresponding to x, y, xy, x(2), and y(2)) is demonstrated for simple objects. The special features of the proposed coherent optical processor and its space-bandwidth requirements are also discussed.
Use of fungal derived polysaccharide-conjugated particles to probe Dectin-1 responses in innate immunity (2011)

The number of life-threatening fungal infections has risen in immunocompromised patients, and identification of the rules that govern an appropriate immune response is essential to develop better diagnostics and targeted therapeutics. The outer cell wall component on pathogenic fungi consists of β-1,3-glucan, and Dectin-1, a pattern recognition receptor present on the cell surface of innate immune cells, binds specifically to this carbohydrate. A barrier in understanding the exact immunological response to pathogen-derived carbohydrate epitopes is the presence of multiple types of carbohydrate moieties on fungal cell walls. To dissect the immunological mechanisms used to recognize pathogens, a system of "fungal like particles" was developed that consisted of polystyrene beads, which mimicked the three dimensional shape of the fungus, coated covalently with purified β-1,3-glucan derived from Saccharomyces cerevisiae. The morphology of the β-1,3-glucan layer was examined by immunofluorescence, flow cytometery, and immuno-transmission electron microscopy. The covalent linkages of the β-1,3-glucan to the polystyrene surface were stable after subjecting the beads to detergents. By pre-treating β-1,3-glucan beads with laminarinase, a specific β-1,3-gluconase, the reactivity of the anti-β-1,3-glucan antibody was abrogated in comparison to treatment with proteinase K indicating that the coating of these beads was predominantly β-1,3-glucan. TNF-α was also measured by stimulating bone-marrow derived macrophages with the β-1,3-glucan beads, and showed a dose dependent response compared to soluble β-glucan, insoluble β-1,3-glucan, uncoated beads, and soluble β-1,3-glucan mixed with uncoated beads. Finally, β-1,3-glucan beads were incubated with GFP-Dectin-1 expressing macrophages and imaged using confocal microscopy. β-1,3-beads were taken up within minutes and retained Dectin-1 recruitment to the phagosome as compared to uncoated beads. These data describe a unique fungal-like particle system that will permit immunologists to probe the critical steps in early recognition of pathogen-derived fungal carbohydrate antigens by innate immune cells.
Novel Solid Encapsulation of Ethylene Gas Using Amorphous α-Cyclodextrin and the Release Characteristics (2016)

This research investigated the encapsulation of ethylene gas into amorphous α-cyclodextrins (α-CDs) at low (LM) and high (HM) moisture contents at 1.0-1.5 MPa for 24-120 h and its controlled release characteristics at 11.2-52.9% relative humidity (RH) for 1-168 h. The inclusion complexes (ICs) were characterized using X-ray diffractometry (XRD), nuclear magnetic resonance spectroscopy (CP-MAS (13)C NMR), and scanning electron microscopy (SEM). Ethylene concentrations in the ICs were from 0.45 to 0.87 mol of ethylene/mol CD and from 0.42 to 0.54 mol of ethylene/mol CD for LM and HM α-CDs, respectively. Ethylene gas released from the encapsulated powder at higher rates with increasing RH. An analysis of release kinetics using Avrami's equation showed that the LM and HM amorphous α-CDs were not associated with significant differences in release constant k and parameter n for any given RH condition. NMR spectra showed the presence of the characteristic carbon-carbon double bond of ethylene gas in the encapsulated α-CD powder.