gebhart/patrepeval huggingface dataset:
Trapping IgE in a closed conformation by mimicking CD23 binding prevents and disrupts FcεRI interaction (2017)

Anti-IgE therapeutics interfere with the ability of IgE to bind to its receptors on effector cells. Here we report the crystal structure of an anti-IgE single-domain antibody in complex with an IgE Fc fragment, revealing how the antibody inhibits interactions between IgE and the two receptors FcεRI and CD23. The epitope overlaps only slightly with the FcεRI-binding site but significantly with the CD23-binding site. Solution scattering studies of the IgE Fc reveal that antibody binding induces a half-bent conformation in between the well-known bent and extended IgE Fc conformations. The antibody acts as functional homolog of CD23 and induces a closed conformation of IgE Fc incompatible with FcεRI binding. Notably the antibody displaces IgE from both CD23 and FcεRI, and abrogates allergen-mediated basophil activation and facilitated allergen binding. The inhibitory mechanism might facilitate strategies for the future development of anti-IgE therapeutics for treatment of allergic diseases.
Micropulse P3™ (MP3) Laser for Glaucoma: An Innovative Therapy (2018)

<b>How to cite this article:</b> Aquino MC, Lim D, Chew PTK. Micropulse P3™(MP3) Laser for Glaucoma: An Innovative Therapy. J Curr Glaucoma Pract 2018;12(2):51-52.
Cardioprotection against ischaemia/reperfusion by vitamins C and E plus <i>n</i>−3 fatty acids: molecular mechanisms and potential clinical applications (2012)

The role of oxidative stress in ischaemic heart disease has been thoroughly investigated in humans. Increased levels of ROS (reactive oxygen species) and RNS (reactive nitrogen species) have been demonstrated during ischaemia and post-ischaemic reperfusion in humans. Depending on their concentrations, these reactive species can act either as benevolent molecules that promote cell survival (at low-to-moderate concentrations) or can induce irreversible cellular damage and death (at high concentrations). Although high ROS levels can induce NF-κB (nuclear factor κB) activation, inflammation, apoptosis or necrosis, low-to-moderate levels can enhance the antioxidant response, via Nrf2 (nuclear factor-erythroid 2-related factor 2) activation. However, a clear definition of these concentration thresholds remains to be established. Although a number of experimental studies have demonstrated that oxidative stress plays a major role in heart ischaemia/reperfusion pathophysiology, controlled clinical trials have failed to prove the efficacy of antioxidants in acute or long-term treatments of ischaemic heart disease. Oral doses of vitamin C are not sufficient to promote ROS scavenging and only down-regulate their production via NADPH oxidase, a biological effect shared by vitamin E to abrogate oxidative stress. However, infusion of vitamin C at doses high enough to achieve plasma levels of 10 mmol/l should prevent superoxide production and the pathophysiological cascade of deleterious heart effects. In turn, n-3 PUFA (polyunsaturated fatty acid) exposure leads to enhanced activity of antioxidant enzymes. In the present review, we present evidence to support the molecular basis for a novel pharmacological strategy using these antioxidant vitamins plus n-3 PUFAs for cardioprotection in clinical settings, such as post-operative atrial fibrillation, percutaneous coronary intervention following acute myocardial infarction and other events that are associated with ischaemia/reperfusion.
Effects of myo-inositol supplementation on oocyte's quality in PCOS patients: a double blind trial. (2011)

These data suggest that myoinositol may be useful in the treatment of PCOS patients undergoing ovulation induction, both for its insulin-sensitizing activity, and its role in oocyte maturation.
Analysis of volatile organic compounds: possible applications in metabolic disorders and cancer screening. (2001)

The human breath contains a variety of endogenous volatile organic compounds (VOCs). The origin and pathophysiological importance of these VOCs is poorly investigated. Little is known about the interaction of VOCs from ambient air, such as those produced by plants and exhaust fumes, with the human organism. Gas chromatographic determination of VOC concentrations is tedious. Proton-transfer-mass spectroscopy (PTR-MS), a new technology for the online detection of VOC patterns, is a valuable alternative. We present two interesting molecular species, isoprene and ortho (o)-toluidine, as examples of endogenously produced VOCs. In a case study, breath isoprene reductions during lipid-lowering therapy (36%) were shown to correlate with cholesterol (32%) and LDL concentrations (35%) in blood (p < 0.001) over a period of 15 days. Therefore, isoprene concentrations in human breath (measured by PTR-MS) might serve as an additional parameter to complement invasive tests for controlling lipid-lowering therapy. Furthermore, it may be a useful parameter for lipid disorder screening. Mass-108, which presumably represents o-toluidine in our breath samples, was found in significantly higher concentrations in the breath of patients with different tumors (1.5 +/- 0.8 ppbv) than in age-matched controls (0.24 +/- 0.1 ppbv, p < 0.001). Inflammatory reactions do not seem to alter the pattern of mass-108. Therefore, it appears to be a currently underestimated carcinoma marker that deserves further investigation.
Physicochemical properties of VPI-5 (1989)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPhysicochemical properties of VPI-5Mark E. Davis, Consuelo Montes, Paul E. Hathaway, Juan P. Arhancet, Dennis L. Hasha, and Juan M. GarcesCite this: J. Am. Chem. Soc. 1989, 111, 11, 3919–3924Publication Date (Print):May 1, 1989Publication History Published online1 May 2002Published inissue 1 May 1989https://doi.org/10.1021/ja00193a024RIGHTS & PERMISSIONSArticle Views640Altmetric-Citations170LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (693 KB) Get e-Alerts Get e-Alerts
The allergens of dog II. Identification and partial purification of a major dander allergen (1992)

A dog hair and dander (DHD) extract was prepared from hair obtained from mixed breeds. By SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting, using sera from 32 dog-allergic subjects, a number of IgE radio-staining bands could be seen. In 78% of sera a protein of molecular weight (MW) of 21,000 daltons, designated Ag X, was found to bind IgE and in 34% it did so strongly. This allergen was isolated from DHD by size-exclusion and ion exchange chromatography. The final product was a single allergen of MW of 21,000 and an isoelectric point of approximately 5.2. An additional protein-staining band could still be seen of MW of 24,000 daltons. Using a serum which contained IgE antibodies only to Ag X, this allergen was found only in DHD extract and dog saliva and was absent from dog serum and urine. It was the same dog allergen that we [1] reported as Ag 8 using crossed radio-immunoelectrophoresis (CRIE) and that Blands et al. [2] and Løwenstein [3] described as Ag 13. We propose that this major dog allergen be given the title Can f I according to the new allergen nomenclature.
Isolation and characterization of the <i>Arabidopsis</i> organ fusion gene <i>HOTHEAD</i> (2003)

The outer epidermal plant cell wall and cuticle play an important role in regulating both abiotic and biotic interactions between the plant and its environment. In addition to acting as a protective barrier that limits water loss, the effects of detrimental irradiation and invasion by pathogens, the epidermis also offers an interface that is inert to interactions between organs and ensures proper separation and expansion of organs at the growing points of the plant. Here, we describe the molecular cloning and characterization of HOTHEAD (HTH), a gene required to limit cellular interactions between contacting epidermal cells during floral development. HTH is a member of a small gene family in Arabidopsis and encodes an enzyme related to a group of FAD-containing oxidoreductases that have been described in several other species. Characterization of 11 independently derived mutant alleles suggests that key amino acids are shared between these related groups of enzymes and identify a cluster of other functionally important residues that are highly conserved only within the Arabidopsis gene family. Our findings add this new type of enzyme to a growing list of enzymes that have been shown to be involved in regulating post-genital organ fusion. Expression analysis of the HTH gene shows that it is expressed in all tissues tested, including roots, and is not epidermis-specific. Furthermore, the sequence data unequivocally show that none of the alleles isolated are epigenetic alleles as suggested by genetic behavior previously observed at this locus.
Inducible carboxypeptidase activity in vancomycin-resistant enterococci (1992)

Vancomycin was found to coinduce DD-carboxypeptidase activity, together with resistance, in eight low- or high-level glycopeptide-resistant strains of enterococci. The constitutively resistant mutant (MT10) of a low-level-resistant strain of Enterococcus faecium (D366) spontaneously expressed a level of carboxypeptidase similar to that of the induced strain D366. Pentapeptide, UDP-MurNac-pentapeptide, as well as D-alanyl-D-alanine were in vitro substrates for the carboxypeptidase which was not inhibited by penicillin. The level of vancomycin resistance correlated roughly with the level of carboxypeptidase activity. We infer from these results that the carboxypeptidase is one component of the glycopeptide resistance mechanism.
The Applications of Cardinal Trigonometric Splines in Solving Nonlinear Integral Equations (2014)

The cardinal trigonometric splines on small compact supports are employed to solve integral equations. The unknown function is expressed as a linear combination of cardinal trigonometric splines functions. Then a simple system of equations on the coefficients is deducted. When solving the Volterra integral equations, the system is triangular, so it is relatively straight forward to solve the nonlinear system of the coefficients and a good approximation of the original solution is obtained. The sufficient condition for the existence of the solution is discussed and the convergence rate is investigated.
Time-domain segmentation based massively parallel simulation for ADCs (2013)

The great availability of massively parallel computing platforms gives rise a question to the EDA industry--how can this be really helping the productivity of circuit designs. Scalability of traditional parallel methods have shown to be limited as the computational resources keep increasing. In this paper we propose a time-domain segmentation method for massively parallel transistor-level simulation for short-memory circuits. SNDR simulation for ADCs is selected as the application as ADCs are typical short-memory circuits and the SNDR simulation is very time consuming. Experiments with realistic Flash and SAR ADCs demonstrate 64x-78x speed-ups with 100 CPU cores. With minor, yet important modifications, the proposed method can even be applied to simulation of Σ-Δ modulator, which does not satisfy the short-memory condition due to the presence of integrator, and 52x speed-up is observed with 100 CPU cores. The implementation of the proposed method is extremely simple and no modification to simulator is needed.
Si photonic integrated circuit for a compact and stable CW-THz spectrometer (2014)

A silicon photonic circuit for continuous-wave terahertz vector spectroscopy greatly reduces the physical size of the system and phase instability. Using off-chip uni-traveling-carrier photodiodes, we demonstrate fast and highly stable spectrometer.
Stereodivergent Construction of Aminodiols with a CF<sub>3</sub> Group (2010)

A stereodivergent synthesis of various 2-amino-1,3-diols and 3-amino-1,2-diols from a single intermediate, 2,3-epoxyketones, is described.
Ensembl 2019 (2018)

The Ensembl project (https://www.ensembl.org) makes key genomic data sets available to the entire scientific community without restrictions. Ensembl seeks to be a fundamental resource driving scientific progress by creating, maintaining and updating reference genome annotation and comparative genomics resources. This year we describe our new and expanded gene, variant and comparative annotation capabilities, which led to a 50% increase in the number of vertebrate genomes we support. We have also doubled the number of available human variants and added regulatory regions for many mouse cell types and developmental stages. Our data sets and tools are available via the Ensembl website as well as a through a RESTful webservice, Perl application programming interface and as data files for download.
Deep learning the dynamic appearance and shape of facial action units (2016)

Spontaneous facial expression recognition under uncontrolled conditions is a hard task. It depends on multiple factors including shape, appearance and dynamics of the facial features, all of which are adversely affected by environmental noise and low intensity signals typical of such conditions. In this work, we present a novel approach to Facial Action Unit detection using a combination of Convolutional and Bi-directional Long Short-Term Memory Neural Networks (CNN-BLSTM), which jointly learns shape, appearance and dynamics in a deep learning manner. In addition, we introduce a novel way to encode shape features using binary image masks computed from the locations of facial landmarks. We show that the combination of dynamic CNN features and Bi-directional Long Short-Term Memory excels at modelling the temporal information. We thoroughly evaluate the contributions of each component in our system and show that it achieves state-of-the-art performance on the FERA-2015 Challenge dataset.
Thermo-Viscoelastic Analysis for Warpage of Ball Grid Array Packages Taking into Consideration of Chemical Shrinkage of Molding Compound (2004)

概要表面実装パッケージの反りの抑制は, パッケージの切断や基板との実装を確実に行い, はんだ接合部の機械的強度信頼性を確保する上で重要な課題である。本研究では, 熱粘弾性解析技術に硬化収縮率を導入したBGAパッケージの反り解析方法を提案した。その結果, 硬化収縮に対応する反りを正確に計算, 低温からリフロー温度までの反りをおおむね予測できることがわかった。材料物性パラメータの解析によって, BGAパッケージ反りの発生メカニズムが明確になり, 反り量を抑制するには, 1) 封止樹脂のTgを上げる, 2) 高温での弾性率の低減が有効であった。
Prostatic Carcinoma (1979)

EACH year there are 42,000 new cases of prostatic carcinoma in the United States,1 and with 17,000 deaths per year, it is the second leading cause of cancer deaths among American men.2 Despite these alarming statistics, it can be calculated that 30 per cent, or roughly 12,000, of the new cases are potentially curable when first discovered.* But it seems that treatment designed to cure patients of this disease is being provided to only a fraction of the potentially curable ones. Consider that one of the most active groups in the country engaged in urologic surgery and oncology attempts cure . . .
Exergy analysis of an operating boiling-water-reactor nuclear power station (1995)

A Second Law analysis is performed on the LaSalle County Nuclear Station of the Commonwealth Edison Company to evaluate plant and subsystem irreversibility. The results disclose that over 80% of the exergy destroyed during plant operation is result of the highly irreversible fission and heat transport processes within the reactor vessel. Plant efficiency and effectiveness are found to be 34.4%, well below the 40–45% efficiencies of typical fossil-fuel-fired power generating stations. Based on these well-known numbers, and the results of the exergy analysis, one recommendation is to reevaluate the integration of fossil-fuel-fired superheat/reheat units located downstream of the reactor vessel. This modified plant configuration would not only improve efficiency by raising the top operating temperature, but it is also anticipated to reduce the irreversibility associated with heat transfer in the steam generators.
The Role of Tumor Markers in Gynecologic Oncology (1990)

Human chorionic gonadotropin is a highly sensitive and specific tumor marker for gestational trophoblastic neoplasia that accurately reflects tumor volume and the clinical course of disease. In women with endodermal sinus tumors and embryonal carcinomas, alpha-fetoprotein usually is a reliable marker, and it accurately predicts the presence of yolk sac elements in mixed germ cell tumors. CA 125 is the most widely utilized tumor marker presently available for use in patients with epithelial ovarian cancer. It has utility in monitoring therapy and differentiating benign from malignant pelvic masses. Measurement of serial CA 125 levels in postmenopausal women may facilitate screening for occult early stage disease. In the future, monoclonal antibodies that recognize tumor-associated antigens may prove useful for radionuclide imaging.
Investigation of Effects and Mechanisms of Total Flavonoids of<i>Astragalus</i>and Calycosin on Human Erythroleukemia Cells (2012)

Flavonoids are found in most parts of plants and have been shown to have multiple biological activities such as anticancer, anti-inflammation, antibacteria, antivirus, and immune-stimulation. Existing data showed that the total flavonoids of Astragalus (TFA) can provide biological system with resistance to injury and can possess antimutagenic, atherosclerotic inhibition, and other biological effects. This study investigated the effects of TFA and calycosin (a compound isolated from TFA), on apoptosis induction, and cell cycle of human erythroleukemia cell line K562 by an array of techniques, including proliferation (MTT), PI staining, Annexin V/PI double staining, and RT-PCR. The experimental data showed that TFA and calycosin could inhibit the proliferation of K562 cells. The 50% inhibiting concentrations of TFA and calycosin were 98.63 μg/mL and 130.32 μg/mL, respectively. However, TFA and calycosin could not induce apoptosis in K562 cells, but could increase the number of the cells in the G(0)/G(1) phase. The level of cyclin D1 mRNA in K562 cells decreased after the treatment with TFA and calycosin. This study provides new insights into the functional mechanism of total flavonoids of Astragalus and calycosin on human erythroleukemia cells.
Oxcarbazepine: Mechanisms of Action (1994)

The antiepileptic drug (AED) oxcarbazepine (OCBZ) and its rapidly formed 10-monohydroxy metabolite (MHD) protect against electroshock-induced tonic hindlimb extension in rodents (ED50 14-21 mg/kg p.o.). Both stereoisomers of MHD also protect. As with carbamazepine (CBZ), these findings suggest clinical efficacy against generalized tonic-clonic and, to some extent, partial seizures. OCBZ (IC50 5 x 10(-8) M), MHD (IC50 2 x 10 (-8) M), and CBZ (IC50 6 x 10(-7) M) limit the frequency of firing of sodium-dependent action potentials by cultured mouse central neurons and reduce Vmax progressively in a use-dependent manner at concentrations below therapeutic plasma concentrations in OCBZ-treated patients. This suggests that blockade of voltage-sensitive sodium channels could contribute to the antiepileptic efficacy of OCBZ. Blockade of penicillin-induced epileptiform discharges in hippocampal slices by MHD and its stereoisomers was diminished when the potassium channel blocker 4-aminopyridine was added to the bath fluid. This indicates that additional mechanisms of action, e.g., an effect on potassium channels, might be clinically important. In addition, both stereoisomers are equally responsible for the antiepileptic activity of the racemate, i.e., MHD, and are therefore likely to play a therapeutic role. Such actions could confer broad clinical utility on OCBZ.
Coarse-grain simulation of viscous flow and stamp deformation in nanoimprint (2007)

A refined coarse-grain software for the simulation of thermal nanoimprint lithography is described. This software takes into account the stamp bending during squeeze flow and is able to provide results well suited for the quantitative prediction of the residual resist thickness with an accuracy better than 10%.
Comparison of Patch-Based Conditional Generative Adversarial Neural Net Models with Emphasis on Model Robustness for Use in Head and Neck Cases for MR-Only planning (2019)

A total of twenty paired CT and MR images were used in this study to investigate two conditional generative adversarial networks, Pix2Pix, and Cycle GAN, for generating synthetic CT images for Headand Neck cancer cases. Ten of the patient cases were used for training and included such common artifacts as dental implants; the remaining ten testing cases were used for testing and included a larger range of image features commonly found in clinical head and neck cases. These features included strong metal artifacts from dental implants, one case with a metal implant, and one case with abnormal anatomy. The original CT images were deformably registered to the mDixon FFE MR images to minimize the effects of processing the MR images. The sCT generation accuracy and robustness were evaluated using Mean Absolute Error (MAE) based on the Hounsfield Units (HU) for three regions (whole body, bone, and air within the body), Mean Error (ME) to observe systematic average offset errors in the sCT generation, and dosimetric evaluation of all clinically relevant structures. For the test set the MAE for the Pix2Pix and Cycle GAN models were 92.4 $\pm$ 13.5 HU, and 100.7 $\pm$ 14.6 HU, respectively, for the body region, 166.3 $\pm$ 31.8 HU, and 184 $\pm$ 31.9 HU, respectively, for the bone region, and 183.7 $\pm$ 41.3 HU and 185.4 $\pm$ 37.9 HU for the air regions. The ME for Pix2Pix and Cycle GAN were 21.0 $\pm$ 11.8 HU and 37.5 $\pm$ 14.9 HU, respectively. Absolute Percent Mean/Max Dose Errors were less than 2% for the PTV and all critical structures for both models, and DRRs generated from these models looked qualitatively similar to CT generated DRRs showing these methods are promising for MR-only planning.
Increased Susceptibility to Atrial Fibrillation Secondary to Atrial Fibrosis in Transgenic Goats Expressing Transforming Growth Factor‐β1 (2016)

A novel transgenic goat model with a substrate for AF was generated. In this model, cardiac overexpression of TGF-β1 led to an increase in fibrosis and myocyte size in the atria, and to progressive P-wave prolongation. We suggest that these factors underlie increased AF susceptibility.
Lossless compression catalyst based on binary allocation via modular arithmetic (2014)

A new binary (bit-level) lossless compression catalyst method based on a modular arithmetic, called Binary Allocation via Modular Arithmetic (BAMA), has been introduced in this paper. In other words, BAMA is for storage and transmission of binary sequences, digital signal, images and video, also streaming and all kinds of digital transmission. As we said, our method does not compress, but facilitates the action of the real compressor, in our case, any lossless compression algorithm (Run Length Encoding, Lempel-Ziv-Welch, Huffman, Arithmetic, etc), that is, it acts as a compression catalyst. Finally, this catalyst allows a significant increase in the compression performance of binary sequences, among others.