gebhart/patrepeval huggingface dataset:
Antiproliferative effect of anti PAR antibody on breast and prostate cancer cells (2005)

550 Previously, we reported the isolation of a novel gene located on chromosome 1 in Epidermal Differentiation Complex (EDC) which encodes a 146 amino acids transmembrane protein that we called Prostate Androgen Regulated (PAR) gene. The PAR gene is expressed as a single mRNA species in all normal tissues studied and is over expressed in majority of tumors, including breast and prostate. The modulation of PAR expression induced by transfection with gene encoding sequence in sense and antisense orientation suggests that PAR is a protooncogene. Here we report the effect of anti P1 (PAR) antibodies on proliferation of MCF 7 and DU 145 cancer cells. Anti P1 antibody is a highly specific, affinity purified rabbit polyclonal antibody raised against a 20 amino acid antigenic peptide (P1) selected from the amino terminal deduced PAR sequence. On Western blots this antibody reacted with P1 peptide, with recombinant PAR protein prepared in E coli, and with lysates from LNCaP and MCF 7 cells, but not with BSA or protein standards. Antibody activity was completely abolished by antibody pre treatment with P1 peptide indicating its specificity. In tissue culture this antibody induced inhibition of cell proliferation of DU 145 and MCF 7 cells in a dose and time dependent manner. At a concentration of 9 μg/ml about 90% of MCF 7 cells were dead within 4-5 days compared with the control cells treated with IgG from normal rabbits. MCF 10F normal breast cells were not affected by the same treatment. When given ip twice a week in dose of 50 μg/mouse the anti P1 antibody significantly inhibited the growth of DU 145 cells tumors in CB17 SCID mice. Anti PAR antibody induced apoptosis in DU145 cells as evidenced by flow cytometric detection of sub-G1 DNA content. This apoptotic process was associated with down-regulation of Bcl-2 and an increase of caspase-3 activity. Further work is carried out in our laboratory to determine the mechanism of antiproliferative effect of these antibodies.
Fast generation of digital holograms based on warping of the wavefront recording plane (2015)

This paper reports a fast method for generating a 2048x2048 digital Fresnel hologram at a rate of over 100 frames per second. Briefly, the object wave of an image is nonuniformally sampled and generated on a wavefront recording plane (WPR) that is close to the object scene. The sampling interval at each point on the WRP image is then modulated according to the depth map. Subsequently, the WRP image is converted into a hologram. The hologram generated with our proposed method, which is referred to as the warped WRP (WWRP) hologram, is capable of presenting a 3-D object with faster speed as compared with existing methods.
Hydrolytically Degradable Polymer Micelles for Anticancer Drug Delivery to Solid Tumors (2012)

Abstract Various biodegradable micellar polymer drug carriers based on N ‐(2‐hydroxypropyl)methacrylamide copolymers and their conjugates with doxorubicin are synthesized. The conjugates are designed to treat solid tumors and subsequently to allow carrier elimination from the body. All copolymers self‐assemble in aqueous solution into supramolecular structures with highly hydrophobic cholesterol derivatives in the core and doxorubicin located in the hydrophilic shell. The drug is released quickly from the micelles incubated at pH = 5.0 (modelling the endosomes of tumor cells). Slower release of cholesterol derivatives leading to disintegration of the micelles is observed.
Detection of Human Papillomavirus in Papanicolaou Smears (1998)

The purpose of this study was to correlate the in situ detection of human papillomavirus (HPV) DNA in archival Papanicolaou (Pap) smears with the pathologic findings and, for atypical squamous cells of undetermined significance (ASCUS), clinical follow-up. Eighty-two Pap smears were destained and analyzed for HPV DNA by in situ hybridization using a consensus probe cocktail that could detect the oncogenic HPV types. "High-risk" HPV DNA was detected in 18 of 23 (78%) low grade SILs, 16 of 40 (40%) ASCUSs, and 1 of 19 (5%) normal Pap smears. The in situ detection of HPV DNA in ASCUS Pap smears with a corresponding biopsy-proven squamous intraepitheal lesion (SIL) within 6 months was significantly greater (14 of 21, 67%) than in smears with corresponding biopsy specimens were negative for SIL (2 of 19, 10%) (p < 0.05). Analysis of the smears negative for HPV using the high-risk probe cocktail with probes for low-risk HPV 6, 11, 42, 43, and 44 increased the percentage of positive smears to 91% (21 of 23) of low grade SILs and 50% (20 of 40) for ASCUS, including 81% (17 of 21) of the women in whom dysplasia developed; there was no change in the percentage of positive cases in normal Pap smears. It is concluded that the in situ detection of HPV DNA in ASCUS cells can help the clinician to differentiate those women at very high risk for a biopsy-proven SIL from those at low risk.
Efficient Lookup Table-Based Adaptive Baseband Predistortion Architecture for Memoryless Nonlinearity (2010)

Digital predistortion is an effective means to compensate for the nonlinear effects of a memoryless system. In case of a cellular transmitter, a digital baseband predistorter can mitigate the undesirable nonlinear effects along the signal chain, particularly the nonlinear impairments in the radiofrequency (RF) amplifiers. To be practically feasible, the implementation complexity of the predistorter must be minimized so that it becomes a cost-effective solution for the resource-limited wireless handset. This paper proposes optimizations that facilitate the design of a low-cost high-performance adaptive digital baseband predistorter for memoryless systems. A comparative performance analysis of the amplitude and power lookup table (LUT) indexing schemes is presented. An optimized low-complexity amplitude approximation and its hardware synthesis results are also studied. An efficient LUT predistorter training algorithm that combines the fast convergence speed of the normalized least mean squares (NLMSs) with a small hardware footprint is proposed. Results of fixed-point simulations based on the measured nonlinear characteristics of an RF amplifier are presented.
<i>In Vitro</i> Investigations of Citrate Clearance with Different Dialysis Filters (2012)

Citrate can efficiently be removed with standard hemodialysis. However, depending on the infused amounts as well as on the patient - especially in patients with impaired liver function - the use of a high-flux dialysis filter and a high dialysate flow rate should be considered to minimize the risk of citrate accumulation.
A Simple Theory and New Method of Differential Beamforming With Uniform Linear Microphone Arrays (2020)

This article presents a theoretical study of differential beamforming with uniform linear arrays. By defining a forward spatial difference operator, any order of the spatial difference of the observed signals can be represented as a product of a difference operator matrix and the microphone array observations. Consequently, differential beamforming is implemented in two stages, where the first one obtains spatial difference of the observations and the second stage optimizes the beamformer. The major contributions of this article are as follows. First, we propose a new theory of differential beamforming with uniform linear arrays, which shows clearly the connection between the conventional differential beamforming and the null-constrained differential beamforming methods. This provides some new insight into the design of differential beamformers. Second, we deduce some new differential beamformers, where conventional beamforming may be seen as a particular case. Specifically, we derive the maximum white noise gain (MWNG), maximum directivity factor (MDF), parameterized MDF, and parameterized maximum front-to-back ratio differential beamformers. Third, we further extend the idea of how to design optimal differential beamformers by combining both the observed signals and their spatial differences.
Crosstalk Cancellation In 3D Video With Local Contrast Reduction (2011)

Publication in the conference proceedings of EUSIPCO, Barcelona, Spain, 2011
Joint Bayesian positioning and multipath mitigation in GNSS (2008)

A sequential Bayesian estimation algorithm for joint positioning and multipath mitigation within satellite navigation receivers is presented. The underlying process model is especially designed for dynamic user scenarios and dynamic channel conditions. To demonstrate its capabilities simulation results are presented.
Cucurbit[<i>n</i>]uril Analogues (2003)

[structure: see text] Cucurbits come in a variety of sizes, shapes, and colors. We present a building block approach that allows the tailor-made synthesis of CB[5], CB[6], and CB[7] analogues whose sizes, shapes, and colors differ from those of the known CB[n].
Network-Aware Feasible Repairs for Erasure-Coded Storage (2018)

A significant amount of research on using erasure coding for distributed storage has focused on reducing the amount of data that needs to be transferred to replace failed nodes. This continues to be an active topic as the introduction of faster storage devices looks to put an even greater strain on the network. However, with a few notable exceptions, most published work assumes a flat, static network topology between the nodes of the system. We propose a general framework to find the lowest cost feasible repairs in a more realistic, heterogeneous and dynamic network, and examine how the number of repair strategies to consider can be reduced for three distinct erasure codes. We devote a significant part of the paper to determining the set of feasible repairs for random linear network coding (RLNC) and describe a system of efficient checks using techniques from the arsenal of dynamic programming. Our solution involves decomposing the problem into smaller steps, memorizing, and then reusing intermediate results. All computationally intensive operations are performed prior to the failure of a node to ensure that the repair can start with minimal delay, based on up-to-date network information. We show that all three codes benefit from being network aware and find that the extra computations required for RLNC can be reduced to a viable level for a wide range of parameter values.
N-Alkylated 1,4-Dihydropyridines: New Agents to Overcome Multidrug Resistance. (1995)

New N-alkylated 1,4-dihydropyridine derivatives were synthesized and their ability to overcome multidrug resistance was examined in vincristine-resistant P388 cells (P388/VCR cells). Compounds that possessed an arylalkyl substituent on the dihydropyridine ring nitrogen were more potent than verapamil in potentiating the cytotoxicity of vincristine against P388/VCR cells. However, neither drug effectively enhanced the antitumor activity of vincristine in tumor-bearing mice. Introduction of basic nitrogen-containing substituents on the side chain of 1,4-dihydropyridines gave improved activity in vitro and in vivo. The piperazine derivative 12c and 12o were more than 10 times as potent as verapamil in vitro. Four compounds selected for in vivo testing showed superior antitumor activity in P388/VCR-bearing mice in combination with vincristine. The structure-activity relationships of the compounds are discussed.
Statistical source expansion for question answering (2011)

A source expansion algorithm automatically extends a given text corpus with related content from large external sources such as the Web. The expanded corpus is not intended for human consumption but can be used in question answering (QA) and other information retrieval or extraction tasks to find more relevant information and supporting evidence. We propose an algorithm that extends a corpus of seed documents with web content, using a statistical model to select text passages that are both relevant to the topics of the seeds and complement existing information.
Toll-like Receptor 4 Polymorphisms and Atherogenesis (2002)

The Asp299Gly TLR4 polymorphism, which attenuates receptor signaling and diminishes the inflammatory response to gram-negative pathogens, is associated with a decreased risk of atherosclerosis. This finding is consistent with the hypothesis that innate immunity may play a part in atherogenesis.
Human Embryonic Stem Cell (hESC)-Derived Hematopoietic Elements Are Capable of Engrafting Primary as well as Secondary Fetal Sheep Recipients. (2004)

Abstract We used transplantation into 10 and 20 pre-immune fetal sheep recipients (55–65 days-old, term: 145 days) to evaluate the in vivo potential of hematopoietic elements derived from hESC. The in utero human/sheep xenograft model has proven valuable in assessing the in vivo hematopoietic activity of stem cells from a variety of fetal and post-natal human sources. Five transplant groups were established. Non-differentiated hESC were injected in one group. In the second and third group, embroid bodies differentiated for 8 days were injected whole or CD34+ cells were selected for injection. In the fourth and fifth group, hESC were differentiated on S17 mouse stroma layer and injected whole or CD34+ cells were selected for injection. The animals were allowed to complete gestation and be born. Bone marrow and peripheral blood samples were taken periodically up to over 12 months after injection, and PCR and flowcytometry was used to determine the presence of human DNA/blood cells in these samples. A total of 30 animals were analyzed. One primary recipient that was positive for human hematopoietic activity was sacrificed and whole bone marrow cells were transplanted into a secondary recipient. We analyzed the secondary recipient at 9 months post-injection by PCR and found it to be positive for human DNA in its peripheral blood and bone marrow. This animal was further challenged with human GM-CSF and human hematopoietic activity was noted by flowcytometry analyses of bone marrow and peripheral blood samples. Further, CD34+ cells enriched from its bone marrow were cultured in methylcellulose and human colonies were identified by PCR. We therefore conclude that hESC are capable of generating hematopoietic cells that engraft in 1° sheep recipients. These cells also fulfill the criteria for long-term engrafting hematopoietic stem cells as demonstrated by engraftment and differentiation in the 20 recipient.
Development of a micro-fiber nickel electrode for nickel-hydrogen cell (1995)

Development of a high specific energy nickel electrode is the main goal of the lightweight nickel electrode program at the NASA Lewis Research Center. The approach has been to improve the nickel electrode by continuing combined in-house and contract efforts to develop a more efficient and lighter weight electrode for the nickel-hydrogen cell. Small fiber diameter nickel plaques are used as conductive supports for the nickel hydroxide active material. These plaques are commercial products and have an advantage of increased surface area available for the deposition of active material. Initial tests include activation and capacity measurements at different discharge levels followed by half-cell cycle testing at 80 percent depth-of-discharge in a low-Earth-orbit regime. The electrodes that pass the initial tests are life cycle-tested in a boiler plate nickel-hydrogen cell before flightweight designs are built and tested.
Absorbable polymer stent technologies for vascular regeneration (2009)

Abstract Stents are structural implants with widespread clinical use in vascular intervention to re‐open stenotic vessels for the treatment of coronary artery disease and peripheral arterial occlusive disease. Apart from their mechanical function, current drug‐eluting stents (DES) utilize local drug delivery from a drug‐incorporated permanent polymer coating to prevent in‐stent restenosis. This delayed closure of the stented vessel is considered one of the major limitations of conventional bare metal stents (BMS). The long‐term safety of DES, however, is still under debate, with reported cases of delayed healing, late thrombosis and hypersensitivity demanding further evolution in this field. A promising approach to circumvent the limitations of first generation DES is the application of degradable polymer coatings in second generation DES, and fully absorbable polymer stents. From a materials and engineering perspective, this paper provides a mini‐review of current clinically relevant DES technology and recent advancements in the development of stents from degradable polymeric materials as an alternative to permanent BMS and DES. This review, includes work on degradable stents and coatings based on blends of polylactic acid and the microbially‐produced poly(4‐hydroxybutyrate). Copyright © 2009 Society of Chemical Industry
Analysis of a high-voltage merged p-i-n/Schottky (MPS) rectifier (1987)

A new operating mode for the merged p-i-n/Schottky (MPS) rectifier structure is analyzed. It is shown that these devices exhibit superior forward-drop and turn-off-speed characteristics. As an example, for the same forward drop, the 400-V MPS rectifier is an order of magnitude faster in switching speed when compared to a p-i-n rectifier. In addition, for equal switching speed, the MPS rectifier has much lower forward drop and leakage current.
Biotechnological Chondroitin a Novel Glycosamminoglycan With Remarkable Biological Function on Human Primary Chondrocytes (2016)

Cartilage tissue engineering, with in vitro expansion of autologus chondrocytes, is a promising technique for tissue regeneration and is a new potential strategy to prevent and/or treat cartilage damage (e.g., osteoarthritis). The aim of this study was (i) to investigate and compare the effects of new biotechnological chondroitin (BC) and a commercial extractive chondroitin sulfate (CS) on human chondrocytes in vitro culture; (ii) to evaluate the anti-inflammatory effects of the innovative BC compared to extractive CS. A chondrogenic cell population was isolated from human nasoseptal cartilage and in vitro cultures were studied through time-lapse video microscopy (TLVM), immunohistochemical staining and cytometry. In order to investigate the effect of BC and CS on phenotype maintainance, chondrogenic gene expression of aggrecan (AGN), of the transcriptor factor SOX9, of the types I and II collagen (COL1A1 and COL1A2), were quantified through transcriptional and protein evaluation at increasing cultivation time and passages. In addition to resemble the osteoarthritis-like in vitro model, chondrocytes were treated with IL-1β and the anti-inflammatory activity of BC and CS was assessed using cytokines quantification by multiplex array. BC significantly enhances cell proliferation also preserving chondrocyte phenotype increasing type II collagen expression up to 10 days of treatment and reduces inflammatory response in IL-1β treated chondrocytes respect to CS treated cells. Our results, taken together, suggest that this new BC is of foremost importance in translational medicine because it can be applied in novel scaffolds and pharmaceutical preparations aiming at cartilage pathology treatments such as the osteoarthritis. J. Cell. Biochem. 117: 2158-2169, 2016. © 2016 The Authors. Journal of Cellular Biochemistry Published by Wiley Periodicals, Inc.
Astrometry and Photometry with Coronagraphs (2006)

We propose a solution to the problem of astrometric and photometric calibration of coronagraphic images with a simple optical device which, in theory, is easy to use. Our design uses the Fraunhofer approximation of Fourier optics. Placing a periodic grid of wires (we use a square grid) with known width and spacing in a pupil plane in front of the occulting coronagraphic focal plane mask produces fiducial images of the obscured star at known locations relative to the star. We also derive the intensity of these fiducial images in the coronagraphic image. These calibrator images can be used for precise relative astrometry, to establish companionship of other objects in the field of view through measurement of common proper motion or common parallax, to determine orbits, and to observe disk structure around the star quantitatively. The calibrator spots also have known brightness, selectable by the coronagraph designer, permitting accurate relative photometry in the coronagraphic image. This technique, which enables precision exoplanetary science, is relevant to future coronagraphic instruments, and is particularly useful for `extreme' adaptive optics and space-based coronagraphy.
An active hearing protection device for musicians (2013)

Professional musicians have to deal with two great problems when wearing hearing protection devices (HPDs): the occlusion effect and the isolation effect. The occlusion effect is an unnatural and annoying perception of one’s own voice when wearing HPDs. It will affect all musicians whose instrument induces vibrations to the skull, including a singer's vocal tracts and instruments mechanically coupled to the head, such as a trumpet or a violin. The isolation effect is the unnatural sensation of being isolated from a given sound environment. It is caused by a non-uniform attenuation of the HPD over the frequency spectrum and the absence of compensation for psychoacoustic factors, such as uneven loudness perception. These two effects cause a shift of perception between the musician's perception and the audience's perception and therefore compromise the musician’s ability to offer a good performance to his audience. This paper presents the design and implementation of an active musician’s HPD featuring a feedback active noise control system for occlusion effect reduction as well as psychoacoustic compensations for isolation effect reduction. The proposed test procedure and preliminary performance assessments are presented to validate both the test procedure and the system for future subjects trials on a larger scale.
Cycle and performance analysis of absorption heat pumps for waste heat utilization (1981)

This report describes a theoretical analysis of a double-stage absorption heat pump cycle, performed as the first step toward the construction of a laboratory working system. The heat pump designed to upgrade low-temperature waste heat by boosting its temperature typically from 60/sup 0/C (140/sup 0/F) to 120/sup 0/C (250/sup 0/F). The heat, whih may be available from a variety of sources, is thereby made useful for industrial and other applications. The system uses part of the low-temperature heat as its energy source and does not need outside power, except for running small auxiliary equipment. The heat pump employs an desorber/evaporator combination in which some hot water from the heat source is evaporated and the vapor is absorbed in a concentrated absorbent solution. The heat of absorption serves to raise the temperature of the rest of the hot water stream. Two stages of the above system are used in series to provide the desired temperature boost. Both are served by one desorber which uses part of the waste heat to concentrate the solution. The study considers both open- and closed-cycle regeneration of the solution. The report describes the operation of the system and defines its performance criteria. Results of a computermore » study show the variation in performance with different design variables. Two working materials, LiBr-water and LiCl-water, are considered. The effect of different operating conditions is evaluated.« less
CXCL13 antibody for the treatment of autoimmune disorders (2015)

We developed a novel therapeutic antibody targeting CXCL13-mediated signaling pathway for the treatment of autoimmune disorders.
Catalytic Enantioselective Olefin Metathesis and Natural Product Synthesis (2010)

This chapter contains sections titled: Introduction Total Synthesis of Natural Products with Enantiomerically Pure Chiral Olefin Metathesis Catalysts Bearing a C2-symmetric Diolate Ligand Enantioselective Synthesis of Quebrachamine through an Exceptionally Challenging RCM Reaction Synthesis of Baconipyrone C by Ru-catalyzed Enantioselective ROCM Conclusions and Future Outlook Acknowledgments References