gebhart/patrepeval huggingface dataset:
A DNA Aptamer That Inhibits the Formation of Unliganded Receptor Dimer and Ligand-Independent Signaling in Cancer Cells (2020)

<p>Growth factor receptors are activated through dimerization by the binding of their ligands and play pivotal roles in normal cell function. However, in cancer cells, the overexpression of receptors often causes the formation of unliganded receptor dimers, which can be activated in a ligand-independent manner. Thus, the unliganded receptor dimer is a promising target to inhibit aberrant signaling in cancer. Here, we report an aptamer that inhibits ligand-independent receptor activation via preventing the formation of unliganded receptor dimer. By biasing the receptor monomer–dimer equilibrium to the monomer, this aptamer inhibited aberrant cell signaling caused by the unliganded receptor dimer. This work presents a new possibility of oligonucleotide-based therapeutics for cancer.</p>
Dual role of glutathione in modulating camptothecin activity: depletion potentiates activity, but conjugation enhances the stability of the topoisomerase I-DNA cleavage complex. (2001)

Depletion of glutathione (GSH) in MCF-7 and MDA-MB-231 cell lines by pretreatment with the GSH synthesis inhibitor buthionine sulfoximine potentiated the activity of 10,11-methylenedioxy-20(S)-camptothecin, SN-38 [7-ethyl-10-hydroxy-20(S)-camptothecin], topotecan, and 7-chloromethyl-10,11-methylenedioxy-20(S)-camptothecin (CMMDC). The greatest potentiation was observed with the alkylating camptothecin CMMDC. Buthionine sulfoximine pretreatment also increased the number of camptothecin-induced DNA-protein crosslinks, indicating that GSH affects the mechanism of action of camptothecin. We also report that GSH interacts with CMMDC to form a stable conjugate, 7-(glutathionylmethyl)-10,11-methylenedioxy-20(S)-camptothecin (GSMMDC), which is formed spontaneously in buffered solutions and in MCF-7 cells treated with CMMDC. GSMMDC was synthesized and found to be nearly as active as 10,11-methylenedioxy-20(S)-camptothecin in a topoisomerase (topo) I-mediated DNA nicking assay. The resulting topo I cleavage complexes were remarkably stable. In cell culture, GSMMDC displayed potent growth-inhibitory activity against U937 and P388 leukemia cell lines. GSMMDC was not active against a topo I-deficient P388 cell line, indicating that topo I is its cellular target. Peptide-truncated analogues of GSMMDC were prepared and evaluated. All three derivatives [7-(gamma-glutamylcysteinylmethyl)-10,11-methylenedioxy-20(S)-camptothecin, 7-(cysteinylglycylmethyl)-10,11-methylenedioxy-20(S)-camptothecin, and 7-(cysteinylmethyl)-10,11-methylenedioxy-20(S)-camptothecin] displayed topo I and cell growth-inhibitory activity. These results suggest that 7-peptidyl derivatives represent a new class of camptothecin analogues.
A Quasi-Optical Polarization-Duplexed Balanced Mixer for Millimeter-Wave Applications (1983)

An integrated planar antenna-mixer structure for use at millimeterwave frequencies is described. A simple but accurate theory of the slot-ring antenna is applied to several experimental devices. Mixer conversion loss of about 6.5 dB was obtained from an X-band model. Measured radiation patterns of structures designed for 65 GHz agree reasonably well with theory.
On indexed data broadcast (1998)

Article On indexed data broadcast Share on Authors: Sanjeev Khanna Dept. of Fundamental Mathematics Research, Bell Laboratories, 700 Mountain Avenue, Murray Hill, NJ Dept. of Fundamental Mathematics Research, Bell Laboratories, 700 Mountain Avenue, Murray Hill, NJView Profile , Shiyu Zhou Dept. of Fundamental Mathematics Research, Bell Laboratories, 700 Mountain Avenue, Murray Hill, NJ Dept. of Fundamental Mathematics Research, Bell Laboratories, 700 Mountain Avenue, Murray Hill, NJView Profile Authors Info & Claims STOC '98: Proceedings of the thirtieth annual ACM symposium on Theory of computingMay 1998 Pages 463–472https://doi.org/10.1145/276698.276859Online:23 May 1998Publication History 22citation309DownloadsMetricsTotal Citations22Total Downloads309Last 12 Months3Last 6 weeks0 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteGet Access
Abstract 2495: Pharmacodynamic Profile of a Novel Chimeric Natriuretic Peptide, CD-NP, as Compared to C-Type Natriuretic Peptide (2007)

Background: C-type natriuretic peptide (CNP), a 22-amino-acid peptide and a ligand of the natriuretic peptide receptor-B (NPR-B), exhibits venodilating, anti-fibrotic, and vascular-regenerating properties but limited renal actions. A Mayo-designed chimeric peptide, CD-NP, which consists of CNP and the C-terminus of Dendroaspis NP, was synthesized to test the hypothesis that CD-NP would possess CNP-like properties with an improved pharmacodynamic profile. Methods: Normal anesthetized dogs were given CD-NP 50 ng/kg/min (n = 10) or an equimolar dose of CNP 29.3 ng/kg/min (n = 9) i.v. for 75 min. Hemodynamic, renal, and hormonal data were obtained pre-infusion (pre-I), at 30 and 60 min I, and post-I, and were compared both within group vs pre-I (mean ± SE, P< 0.05*, < 0.01 † ) and between groups ( P < 0.05 ‡ , < 0.01 § , < 0.001 ¶ ). Plasma BNP was measured by radioimmunoassay. Results: CD-NP resulted in greater increases in plasma cGMP (7±.4 to 25±3 †¶ to 36±3 †¶ to 23±3 †¶ pmol/ml), urinary cGMP excretion (978±145, 3170±205 †¶ , 5919±616 †¶ , 3077±298 †¶ pmol/min) vs CNP (8±.8, 10±.6 † , 11±.7 † , 8±.5 pmol/ml; 976±110, 1104±115, 1317±103, 998±101 pmol/min, respectively). CD-NP also increased urine Na + excretion (19±4, 168±24 †§ , 237±26 †¶ , 96±12 † μEq/min), urine flow (0.2±.1, 1.3±.2 † , 1.8±.3 † , 0.8±.2 † ml/min), and GFR (37±2 ‡ , 48±3 † , 51±3 † , 53±4 † ml/min) vs CNP (36±12, 68±10, 85±26, 81±25 μEq/min; 0.4±.1, 0.9±.2, 1.2±.3*, 0.9±.3 ml/min; 52±5, 53±7, 50±4, 49±6 ml/min, respectively). CD-NP reduced PCWP (5.7±.7, 4.1±1*, 3.2±.7 † , 4.3±.8 mmHg), RAP (1.8±.4, 1.1±.4 † , 0.9±.5 †§ , 1.3±.5 § mmHg), PAP (12±.6, 10±.4*, 10±.6, 11±.7 mmHg) vs CNP (6±.6, 5±.8, 6±.8, 7±.9 † mmHg; 2.6±.3, 2.5±.3 to 2.9±.3 to 3.5±.5 † mmHg; 13±1, 12±1, 12±1, 13±1 mmHg, respectively). Mean blood pressure was unchanged in either group. CD-NP increased circulating BNP during infusion vs CNP (40±4 vs 18±2 pg/ml, P<0.001). Conclusion: This study demonstrates the successful transformation of CNP to a CNP-like peptide with enhanced natriuretic, diuretic, GFR-enhancing, and cardiac-unloading actions. CD-NP may also promote increases in plasma BNP which via the NPR-A receptor may contribute to its biological actions. The therapeutic potential of CD-NP in heart failure warrants further studies.
[EMmim][NTf<sub>2</sub>]—a Novel Ionic Liquid (IL) in Catalytic CO<sub>2</sub> Capture and ILs’ Applications (2022)

Ionic liquids (ILs) have been used for carbon dioxide (CO<sub>2</sub> ) capture, however, which have never been used as catalysts to accelerate CO<sub>2</sub> capture. The record is broken by a uniquely designed IL, [EMmim][NTf<sub>2</sub> ]. The IL can universally catalyze both CO<sub>2</sub> sorption and desorption of all the chemisorption-based technologies. As demonstrated in monoethanolamine (MEA) based CO<sub>2</sub> capture, even with the addition of only 2000 ppm IL catalyst, the rate of CO<sub>2</sub> desorption-the key to reducing the overall CO<sub>2</sub> capture energy consumption or breaking the bottleneck of the state-of-the-art technologies and Paris Agreement implementation-can be increased by 791% at 85 °C, which makes use of low-temperature waste heat and avoids secondary pollution during CO<sub>2</sub> capture feasible. Furthermore, the catalytic CO<sub>2</sub> capture mechanism is experimentally and theoretically revealed.
Molecular enhancement of porcine cardiac chronotropy (2001)

The present studies showed in a large animal model that local targeting of gene expression may be a feasible modality to regulate cardiac pacemaking activity. In addition, these investigations provide an experimental basis for developing future clinical gene transfer approaches to upregulate heart rate and modulate cardiac conduction.
Microlens characterization by digital holographic microscopy with physical spherical phase compensation (2010)

Microlenses have been characterized by a digital holographic microscopy system, which is immune to the inherent wavefront aberration. The digital holographic microscopy system takes advantage of fiber optics and uses the light emitted directly from a single-mode fiber as the recording reference wave. By using such a reference beam, which is quasi-identical to the object beam, the inherent wavefront aberration of the digital holographic microscope is removed. The alignment of the optical setup can be optimized with the help of numerical reconstruction software to give the system phase with the off-axis tilt removed. There is one, and only one, reference fiber point position to give a reference wavefront that is quasi-identical to the object wavefront where the system is free of wavefront aberration and directly gives the quantitative phase of the test object without the need for complicated numerical compensation.
Techno-Economic Assessment of Integrated Chemical Looping Air Separation for Oxy-Fuel Combustion: An Australian Case Study (2015)

A techno-economic analysis was carried out to assess the oxy-fuel conversion of eight major coal-fired power plants in the state of NSW, Australia. For this purpose, several alternative retrofit configurations, differing only in the air separation unit (ASU) but otherwise identical, were considered. More specifically, three types of oxygen plants were studied: a cryogenic-based air separation unit and integrated chemical looping air separation units using steam (ICLAS[S]) and recycled flue gas (ICLAS[FG]) as the reduction medium. The main objective of the techno-economic analysis was to determine if the economic viability of oxy-fuel operations could be enhanced by incorporating ICLAS technology. The results show that the normalized oxygen demand for the NSW fleet of coal-fired power plants was about 450–550 m3/MWh, with Bayswater having the lowest normalized oxygen demand and Munmorah having the highest one. Moreover, it was found that by replacing a cryogenic-based ASU with an ICLAS unit, the average reduction in the ASU power demand was up to 47% and 76%, respectively, for ICLAS[S] and ICLAS[FG]. Similarly, the average thermal efficiency penalty associated with the cryogenic and the ICLAS[S] and ICLAS[FG] units was found to be about 9.5%, 7.5%, and 5%, respectively, indicating that the ICLAS[FG] unit is the most energy efficient option for oxy-fuel plants. Economic analyses suggest that a retrofit cost reduction of about 32% can be achieved by incorporating an ICLAS[FG] unit. On average, the levelized cost of electricity associated with the cryogenic and the ICLAS[S] and ICLAS[FG] units for the NSW fleet of coal-fired power plants was found to be about $118/MWh, $105/MWh, and $95/MWh, respectively.
Direct Formation of Hydrogen Peroxide with$ H_2$ and $O_2$ over Anthraquinone Encapsulated Pd-Y Zeolite (1999)

Hydrogen peroxide is known as a green oxidizer and uti-lized as a mild oxidant in many oxidation reactions.1 Tradi-tionally, hydrogen peroxide has been commercially produced by the anthraquinone process known as AO process2 which comprises cyclic hydrogenation and oxida-tion of alkylanthraquinones in an appropriate working solu-tion. The product hydrogen peroxide is extracted with water from working solution and the corresponding aqueous per-oxide solution is possibly contaminated with organics. The hydrogen peroxide should be used for many industrial appli-cations after removal of organics due to environmental issues. Those are the main reason why the AO process is characterized as being complex and capital intensive. Recent efforts are concentrated on the direct synthesis of H2O2 with H2 and O2 over heterogeneous catalysts3,4 and by
Geranylgeranyltransferase I Inhibitors Target RalB To Inhibit Anchorage-Dependent Growth and Induce Apoptosis and RalA To Inhibit Anchorage-Independent Growth (2007)

Geranylgeranyltransferase I inhibitors (GGTIs) are presently undergoing advanced preclinical studies and have been shown to disrupt oncogenic and tumor survival pathways, to inhibit anchorage-dependent and -independent growth, and to induce apoptosis. However, the geranylgeranylated proteins that are targeted by GGTIs to induce these effects are not known. Here we provide evidence that the Ras-like small GTPases RalA and RalB are exclusively geranylgeranylated and that inhibition of their geranylgeranylation mediates, at least in part, the effects of GGTIs on anchorage-dependent and -independent growth and tumor apoptosis. To this end, we have created the corresponding carboxyl-terminal mutants that are exclusively farnesylated and verified that they retain the subcellular localization and signaling activities of the wild-type geranylgeranylated proteins and that Ral GTPases do not undergo alternative prenylation in response to GGTI treatment. By expressing farnesylated, GGTI-resistant RalA and RalB in Cos7 cells and human pancreatic MiaPaCa2 cancer cells followed by GGTI-2417 treatment, we demonstrated that farnesylated RalB, but not RalA, confers resistance to the proapoptotic and anti-anchorage-dependent growth effects of GGTI-2417. Conversely, farnesylated RalA but not RalB expression renders MiaPaCa2 cells less sensitive to inhibition of anchorage-independent growth. Furthermore, farnesylated RalB, but not RalA, inhibits the ability of GGTI-2417 to suppress survivin and induce p27(Kip1) protein levels. We conclude that RalA and RalB are important, functionally distinct targets for GGTI-mediated tumor apoptosis and growth inhibition.
Adenovirus Type 5 E1A Gene Products Act as Transformation Suppressors of the <i>neu</i> Oncogene (1991)

The adenovirus type 5 early region 1A (E1A) gene was introduced into neu-transformed B104-1-1 cells. Cells that expressed E1A possessed reduced transforming activity in vitro and reduced tumorigenicity in nude mice. These results demonstrate that the E1A gene products can act negatively to suppress the transformed phenotype in neu-transformed cells.
Silver enhancement of nanogold and undecagold (1995)

A recent advance in immunogold technology has been the use of molecular gold instead of colloidal gold. A number of advantages are realized by this approach, such as stable covalent, site-specific attachment, small probe size and absence of aggregates for improved penetration. Silver enhancement has led to improved and unique results for electron and light microscopy, as well as their use with blots and gels. Most previous work with immunogold silver staining has been done with colloidal gold particles. More recently, large gold compounds (``clusters``) having a definite number of gold atoms and defined organic shell, have been used, frequently with improved results. These gold dusters, large compared to simple compounds, are, however, at the small end of the colloidal gold scale in size; undecagold is 0.8 nm and Nanogold is 1.4 nm. They may be used in practically all applications where colloidal gold is used (Light and electron microscopy, dot blots, etc.) and in some unique applications, where at least the larger colloidal golds don`t work, such as running gold labeled proteins on gels (which are later detected by silver enhancement). The main differences between gold clusters and colloidal golds are the small size of the dusters and their covalent attachment to antibodies or other molecules.
Optical Cloak of Invisibility (2007)

We present the design and analysis of an optical cloak of invisibility with non-magnetic metamaterials. The general recipe for the implementation of such a device is provided. The cloaking performance is illustrated with finite-element simulations.
Photonic Switching of Photoinduced Electron Transfer in a Dihydropyrene−Porphyrin−Fullerene Molecular Triad (2004)

Photonic control of photoinduced electron transfer has been demonstrated in a dimethyldihydropyrene (DHP) porphyrin (P) fullerene (C(60)) molecular triad. In the DHP-P-C(60) form of the triad, excitation of the porphyrin moiety is followed by photoinduced electron transfer to give a DHP-P(*)(+)-C(60)(*)(-) charge-separated state, which evolves by a charge shift reaction to DHP(*)(+)-P-C(60)(*)(-). This final state has a lifetime of 2 micros and is formed in an overall yield of 94%. Visible (>or=300 nm) irradiation of the triad leads to photoisomerization of the DHP moiety to the cyclophanediene (CPD). Excitation of the porphyrin moiety of CPD-P-C(60) produces a short-lived (<10 ns) CPD-P(*)(+)-C(60)(*)(-) state, but charge shift to the CPD moiety does not occur, due to the relatively high oxidation potential of the CPD group. Long-lived charge separation is not observed. Irradiation of CPD-P-C(60) with UV (254 nm) light converts the triad back to the DHP form. Thermal interconversion of the DHP and CPD forms is very slow, photochemical cycling is facile, and in the absence of oxygen, many cycles may be performed without substantial degradation. Thus, light is used to switch long-lived photoinduced charge separation on or off. The principles demonstrated by the triad may be useful for the design of molecule-based optoelectronic systems.
Lupeol induces FAS-mediated apoptotic death of androgen-dependent LNCaP prostate cancer cells and inhibits tumor growth in athymic nude mouse model (2005)

4290 In prostate cancer (CaP) a fine balance between cell proliferation and apoptotic death is lost resulting in increased cellular mass and tumor progression. One approach to redress this imbalance and control this malignancy is its preventive intervention through use of dietary natural agents. Lupeol, a triterpene, is a potent anti-inflammatory agent and recently we have shown that it is an effective anti-tumor promoting agent in mouse skin tumor model (Saleem et al, Oncogene, 23: 5203-5214, 2004,). Here, we investigated the growth-inhibitory effect and associated mechanisms of Lupeol in androgen-dependent human prostate carcinoma LNCaP cells. Lupeol (5-30 μM) treatment resulted in significant inhibition of cell viability at 48 and 72 h post treatment in a time and dose dependent manner. Flow cytometric and immunoblot studies revealed that Lupeol mediated cell death was due to apoptosis. Lupeol was found to induce the cleavage of PARP protein and degradation of Acinus protein with no change in Bcl2, Bax and Caspase 7 protein expression suggesting involvement of a mitochondria-independent apoptotic pathway. In Lupeol treated cells, an increase in the expression of FADD protein was observed. Since FADD acts as an adapter protein for many death inducing signaling molecules such as TNFR-1, APO-3, DR-5, and FAS receptor proteins, we determined their expression levels to establish Lupeol specificity. Lupeol caused a significant increase in the expression of FAS receptor protein with no change in the protein expression of TRADD and TNFR-1, APO-3 and DR-5 receptors. In addition, no significant change in the expression of cell death-inducing receptor ligands such as FAS-L, APO-3L and TRAIL was observed. In parallel experiments, treatment of cells with a combination of a Fas-agonist and Lupeol resulted in an increased cell death as compared to the additive effect of the two compounds alone, suggesting a synergistic effect. A possible explanation for this observation could be that Lupeol might be directly activating FAS protein. Next, we determined whether Lupeol could inhibit the CaP cell tumor growth in an athymic nude mouse xenografts model. The mice were implanted with androgen-dependent CWR22Rν1 cells and received an i.p. injection of Lupeol (1 mg/animal) three times a week. A significant reduction in tumor growth was observed in Lupeol treated mice as compared with untreated control. Taken together, these molecular alterations provide an insight into Lupeol-caused growth inhibition and apoptotic death of human CaP cells. Because early clinical CaP growth is an androgen-dependent response, the results of the present study employing androgen-dependent LNCaP cells suggest that Lupeol has promise and potential to be an effective agent against CaP.
Capacitive micromachined ultrasonic transducers (cmuts) with piston-shaped membranes (2006)

Abstract— Compared to PZT transducers in medical applications, CMUTs reported on so far have broader fractional bandwidth (FBW) but lower transduction efficiency (TX and RX) [1]. Most fabricated CMUTs reported in the literature carried membranes of uniform thickness. Since there is a performance trade-off between transduction efficiency and FBW when designing CMUTs with uniform membrane thickness, there is limited room for performance improvement in these devices. However, wafer-bonding-based CMUT fabrication provides design flexibility by allowing fabrication of membranes with different thickness profiles. Herein, CMUTs featuring piston-shaped membranes are developed to improve device performance. According to our theoretical predictions, piston-shaped membranes should improve the CMUT performance in terms of output pressure, sensitivity, and broader fractional bandwidth. The large ratio of second resonant harmonic frequency to first resonant frequency improves FBW. Increased electrical field intensity in the CMUT cavity (due to the larger equivalent spring constant of this membrane, compared to classical membranes) improves TX and RX. The device performance also benefits from a flatter membrane shape that allows greater average membrane displacement and intra-cavity electrostatic pressure. CMUTs featuring piston-shaped membranes with different geometric shapes were designed and fabricated. In order to make a fair comparison, all designs have a similar first resonant frequency, and all devices are equal in size. Fabricating CMUTs featuring piston-shaped membranes is a more complex process than fabricating CMUTs with uniformly thick membranes. However, no yield-loss was observed when CMUTs featuring piston-shaped membranes with different geometric shapes were fabricated. The device characterization was carried out with both pitch-catch (PC) and pulse-echo (PE) immersion tests in oil. These devices achieved ~100% improvement in transduction performance (TX and RX) over CMUTs with uniform membrane thickness. For CMUTs with square and rectangular membranes, FBW increased from ~110% to ~150% and from ~140% to ~175%, respectively, over CMUTs with uniformly thick membranes. The new devices produced a maximum output pressure exceeding 1 MPa. Finally, performance optimization using the geometric shape was the same in both CMUTs featuring piston-shaped membranes and CMUTs with uniform membrane thickness.