gebhart/patrepeval huggingface dataset:
Side effects of chaperone gene co-expression in recombinant protein production (2010)

Insufficient availability of molecular chaperones is observed as a major bottleneck for proper protein folding in recombinant protein production. Therefore, co-production of selected sets of cell chaperones along with foreign polypeptides is a common approach to increase the yield of properly folded, recombinant proteins in bacterial cell factories. However, unbalanced amounts of folding modulators handling folding-reluctant protein species might instead trigger undesired proteolytic activities, detrimental regarding recombinant protein stability, quality and yield. This minireview summarizes the most recent observations of chaperone-linked negative side effects, mostly focusing on DnaK and GroEL sets, when using these proteins as folding assistant agents. These events are discussed in the context of the complexity of the cell quality network and the consequent intricacy of the physiological responses triggered by protein misfolding.
Primary culture of ovine mammary epithelial cells (1996)

Conference Article| August 01 1996 Primary culture of ovine mammary epithelial cells LYNN M.B. FINCH; LYNN M.B. FINCH 1Hannah Research Institute, Ayr KA6 5HL, UK Search for other works by this author on: This Site PubMed Google Scholar VARRIE A. CRAIG; VARRIE A. CRAIG 1Hannah Research Institute, Ayr KA6 5HL, UK Search for other works by this author on: This Site PubMed Google Scholar ALEX J. KIND; ALEX J. KIND *PPL Therapeutics Limited, Roslin, Edinburgh EH25 9PP, UK Search for other works by this author on: This Site PubMed Google Scholar ANGELIKA SCHNIEKE; ANGELIKA SCHNIEKE *PPL Therapeutics Limited, Roslin, Edinburgh EH25 9PP, UK Search for other works by this author on: This Site PubMed Google Scholar ANGELA SCOTT; ANGELA SCOTT *PPL Therapeutics Limited, Roslin, Edinburgh EH25 9PP, UK Search for other works by this author on: This Site PubMed Google Scholar MICHELLE WELLS; MICHELLE WELLS 1Hannah Research Institute, Ayr KA6 5HL, UK Search for other works by this author on: This Site PubMed Google Scholar COLIN J. WILDE COLIN J. WILDE 1Hannah Research Institute, Ayr KA6 5HL, UK Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1996) 24 (3): 369S. https://doi.org/10.1042/bst024369s Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share MailTo Twitter LinkedIn Cite Icon Cite Get Permissions Citation LYNN M.B. FINCH, VARRIE A. CRAIG, ALEX J. KIND, ANGELIKA SCHNIEKE, ANGELA SCOTT, MICHELLE WELLS, COLIN J. WILDE; Primary culture of ovine mammary epithelial cells. Biochem Soc Trans 1 August 1996; 24 (3): 369S. doi: https://doi.org/10.1042/bst024369s Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search This content is only available as a PDF. © 1996 Biochemical Society1996 Article PDF first page preview Close Modal You do not currently have access to this content.
Monolithically integrated InP-based DBR lasers with an intra-cavity ring resonator (2019)

We investigate the effect of a ring resonator on the linewidth and output spectrum of monolithically integrated extended cavity multi-section DBR lasers with an intra-cavity ring resonator. The goal is to achieve an understanding of whether and how the use of an additional ring filter improves the performance of a DBR laser on the aspects of the SMSR and intrinsic linewidth using the capabilities of the InP active-passive integration platform. The laser output spectrum is in good agreement with our theoretical calculations from a steady-state spectral model. A side-mode suppression ratio between 60 and 70 dB is measured for a range of operating semiconductor optical amplifier currents. The frequency noise power spectral density is measured for a range of output power levels. A minimum intrinsic linewidth of 63 kHz is reported. We compare the measured Lorentzian linewidths with our theoretical expectations and present estimates of the possible linewidth improvement with the available photonic integration technology used in this work.
E-mode cutoff wave number of circular waveguide loaded with dielectric sector (1991)

The E-mode cutoff wave numbers of circular waveguide loaded with dielectric sectors were evaluated theoretically using the null-field formulation. These values are found to agree closely with cutoff wave numbers measured by using the transverse resonance technique.
Hydrogen Radical Additions to Unsaturated Hydrocarbons and the Reverse β‐Scission Reactions: Modeling of Activation Energies and Pre‐Exponential Factors (2009)

The group additivity method for Arrhenius parameters is applied to hydrogen addition to alkenes and alkynes and the reverse beta-scission reactions, an important family of reactions in thermal processes based on radical chemistry. A consistent set of group additive values for 33 groups is derived to calculate the activation energy and pre-exponential factor for a broad range of hydrogen addition reactions. The group additive values are determined from CBS-QB3 ab-initio-calculated rate coefficients. A mean factor of deviation of only two between CBS-QB3 and experimental rate coefficients for seven reactions in the range 300-1000 K is found. Tunneling coefficients for these reactions were found to be significant below 400 K and a correlation accounting for tunneling is presented. Application of the obtained group additive values to predict the kinetics for a set of 11 additions and beta-scissions yields rate coefficients within a factor of 3.5 of the CBS-QB3 results except for two beta-scissions with severe steric effects. The mean factor of deviation with respect to experimental rate coefficients of 2.0 shows that the group additive method with tunneling corrections can accurately predict the kinetics and is at least as accurate as the most commonly used density functional methods. The constructed group additive model can hence be applied to predict the kinetics of hydrogen radical additions for a broad range of unsaturated compounds.
Gaze guided object recognition using a head-mounted eye tracker (2012)

Wearable eye trackers open up a large number of opportunities to cater for the information needs of users in today's dynamic society. Users no longer have to sit in front of a traditional desk-mounted eye tracker to benefit from the direct feedback given by the eye tracker about users' interest. Instead, eye tracking can be used as a ubiquitous interface in a real-world environment to provide users with supporting information that they need. This paper presents a novel application of intelligent interaction with the environment by combining eye tracking technology with real-time object recognition. In this context we present i) algorithms for guiding object recognition by using fixation points ii) algorithms for generating evidence of users' gaze on particular objects iii) building a next generation museum guide called Museum Guide 2.0 as a prototype application of gaze-based information provision in a real-world environment. We performed several experiments to evaluate our gaze-based object recognition methods. Furthermore, we conducted a user study in the context of Museum Guide 2.0 to evaluate the usability of the new gaze-based interface for information provision. These results show that an enormous amount of potential exists for using a wearable eye tracker as a human-environment interface.
An error concealment algorithm for images subject to channel errors (1995)

We present an algorithm to conceal bit errors in still images and image sequences that are coded using the discrete cosine transform (DCT) and variable length codes (VLCs). No modification is necessary to an existing encoder, and no additional bit rate is required. The concealment algorithm is kept simple so that real-time decoding and concealment is possible. A single bit error in these images can cause a block to split into several blocks or several blocks to merge into one. This causes the DCT coefficients of all subsequent blocks to be correctly decoded but stored in the wrong location in the image. Furthermore, the DC coefficient of all subsequent blocks may be incorrect. The error concealment algorithm uses transform domain information to identify the location of the affected blocks and to correct errors. The image quality after error concealment is shown to be significantly improved.
Diagnostic usefulness of five screening assays for HIV in an east African city where prevalence of infection is low (1989)

Five commercial screening assays for HIV-1, evaluated for their usefulness in detecting infection in high-risk groups in the East African country of Djibouti, produced varying degrees of performance when compared to Western blot and immunofluorescence confirmatory assays. In this population with a low prevalence of HIV infection (16/599), two enzyme-linked immunosorbent assays (ELISA; Abbott and Elavia) and two rapid assays (cambridge latex agglutination and Du Pont's HIV-CHEK) exhibited less than optimal sensitivities. However, with the exception of Elavia, these assays displayed excellent specificities. The fifth test (Serodia gelatin agglutination) produced the highest sensitivity (0.938) and negative predictive value but the lowest specificity and positive predictive value of all five tests. False positive reactions, which occurred only with the Elavia and Serodia tests, could not be explained on the basis of early infection in patients or cross-reactions with the related retroviruses HIV-2 and HTLV-I. We conclude that none of the five tests alone is sufficient in this testing situation, but that a combination of tests could satisfy most requirements for identifying HIV-1 reactive and non-reactive sera.
The effect of thermal performance on device current utilisation (2002)

A significant improvement in power semiconductor device utilisation seems possible if the maximum output current of power stages could be limited by thermal feedback from the power converter. To illustrate the potential utilisation benefit obtained in main switching devices, the variation in usable current with operating conditions is examined for several devices applied in a constant frequency PWM VVVF inverter, and operated under conditions to keep their junction temperature constant.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Randomized clinical trial of the impact of insulin therapy on liver function in patients undergoing major liver resection (2013)

NCT00774098 (http://www.clinicaltrials.gov).
Monte Carlo Simulation of Switching Dynamics in Polycrystalline Ferroelectric Capacitors (2019)

Ferroelectric (FE) materials are being studied for a variety of applications in memory, logic, and neuromorphic computing, for which predictive models of FE polarization are essential. In this paper, we present a Monte Carlo simulation framework capable of predicting the dynamic, history-dependent response of an FE under arbitrary input waveforms. The simulation is developed by generalizing the physics-based nucleation-limited switching model for polarization reversal in a polycrystalline FE. Measured polarization reversal data from fabricated FE Hf <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">0.5</sub> Zr <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">0.5</sub> O <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> capacitors are used to extract the statistical distribution of FE grains. After parameter extraction, the model is able to predict the dynamics of the FE capacitor without further calibration. Finally, the model is applied to characterize the dynamic response of FE-dielectric bilayer structures and quantify the reduction in memory window due to device variability.
Mechanically tunable multiphoton fabricated protein hydrogels investigated using atomic force microscopy (2010)

Recent work has demonstrated the feasibility of employing three dimensional (3D) protein hydrogels, fabricated using multiphoton-induced photochemistry, as chemically responsive microactuators in “lab on a chip” devices. In addition, these materials show great promise as cell capture/incubation devices, allowing single bacterial cells to reproduce into multicellular constructs with “user-defined” 3D geometries. However, to date, the mechanical properties of these materials, critical for these applications, have not been quantitatively characterized. In this work, we develop and apply a method to measure the elastic modulus of microfabricated protein hydrogels in situ under dynamic physical and chemical environments. We fabricated protein microcantilevers using a wide range of protein building blocks (albumin, lysozyme, avidin) and probed their mechanical properties using atomic force microscopy (AFM). The length dependence of the spring constant displayed by protein cantilevers followed the predicted cantilever model, yielding the elastic modulus of the material. By varying laser dwell time, the modulus of protein cantilevers could be tuned over 2 orders of magnitude (from 0.03 to 3 MPa for albumin), a range that encompasses modulus values for a number of biological tissues (e.g., cartilage, basement membrane). Further, the modulus was shown to vary strongly over a range of pH values (pH 2–12). Distinct profiles of pH vs. modulus for albumin, lysozyme and avidin cantilevers were observed, which correlate to structural transitions of the incorporated protein. Modification of protein cantilevers via ligand binding (biotin to avidin), increased cantilever stiffness. Finally, using the modulus of a hydrogel microchamber calculated in situ, we determined the pressure generated by a replicating bacterial colony entrapped in the microchamber to be 2.7 ± 1.3 kPa. This work demonstrates an ability to quantify mechanical properties under both chemically and biologically dynamic microenvironments and will enable the development of a robust platform to investigate cell/microenvironmental interactions with high spatial resolution, in three dimensions, using mechanically tunable biological materials.
Multiparameter Intelligent Monitoring in Intensive Care II: A public-access intensive care unit database* (2011)

MIMIC-II documents a diverse and very large population of intensive care unit patient stays and contains comprehensive and detailed clinical data, including physiological waveforms and minute-by-minute trends for a subset of records. It establishes a new public-access resource for critical care research, supporting a diverse range of analytic studies spanning epidemiology, clinical decision-rule development, and electronic tool development.
Effect of quercetin on the anti-tumor activity of cisplatin in EMT6 breast tumor-bearing mice (2019)

The enhanced antitumor effect of cisplatin and decreased renal toxicity after quercetin treatment suggested the applicability of quercetin as an adjuvant for chemotherapeutic agents.
A Connectionist Approach to the Recognition of Trends in Time Ordered Medical Parameters (1989)

Recognition of trends that emerge over time is a capability that successful medical systems must possess. In this paper the network architecture introduced by Elman [4] for predicting successive elements of a sequence is reviewed. It is proposed that a similar architecture can be utilized in a medical domain. The simple example of a diabetic patient receiving a continuous insulin infusion, for whom only the current serum glucose and infusion status is known is suggested as a test problem. Two networks are described, that when presented with the serum glucose and pump settings at time steps t and t+1, are capable of predicting the serum glucose, and suggesting a pump setting at time t+2. Possible applications, and the limitations of this model are then discussed.
Monoamine transmitters and cAMP stimulation of Na transport in freshwater mussels (1982)

The unionid mussels Ligumia subrostrata and Carunculina texasensis maintain a sodium steady state in artificial pond water. When the mussels were injected with serotonin, catecholamines, or phenylephrine (< 2 × 10 −5 M/L blood), the influx of Na was elevated 150–220% above controls and the animals accumulated Na from the medium. A similar response was observed with injections of dibutyryl cyclic AMP. Isolated gills of mussels accumulate Na from a pond-water bathing medium and the Na transport rate was stimulated by serotonin but not catecholamines. Serotonin was present in the gill tissue (2.26 ± 0.18 μg/g wet gill). Serotonergic neurons innervated by adrenergic fibers may be directly stimulating the Na transporting tissues in the gill of freshwater mussels.
Study of bulk and elementary screw dislocation assisted reverse breakdown in low-voltage (&gt;250 V) 4H-SiC p/sup +/-n junction diodes. I. DC properties (1999)

Given the high-density (/spl sim/10/sup 4/ cm/sup -2/) of elementary screw dislocations (Burgers vector=1c with no hollow core) in commercial SiC wafers and epilayers, all large current (>1 A) SiC power devices will likely contain elementary screw dislocations for the foreseeable future. It is therefore important to ascertain the electrical impact of these defects, particularly in high-field vertical power device topologies where SiC is expected to enable large performance improvements in solid-state high-power systems. This paper compares the dc-measured reverse-breakdown characteristics of low-voltage (<250 V) small-area (<5/spl times/10/sup -4/ cm/sup 2/) 4H-SiC p/sup +/-n diodes with and without elementary screw dislocations. Diodes containing elementary screw dislocations exhibited higher pre-breakdown reverse leakage currents, softer reverse breakdown current-voltage (I-V) knees, and highly localized microplasmic breakdown current filaments compared to screw dislocation-free devices. The observed localized 4H-SiC breakdown parallels microplasmic breakdown observed in silicon and other semiconductors, in which space-charge effects limit current conduction through the local microplasma as reverse bias is increased.
Effective driving force applied on DNA inside a solid-state nanopore (2012)

A detailed understanding of the origin of the electrophoretic force on DNA molecules in a solid-state nanopore is important for the development of nanopore-based sequencing technologies. Because of the discrepancies between recent attempts to predict this force and both direct and indirect experimental measurements, this topic has been the focus of much recent discussion. We show that the force is predictable to very good accuracy if all of the experimental conditions are accounted for properly. To resolve this issue, we compare the calculation efficiency and accuracy of numerical solutions of Poisson-Boltzmann and Poisson-Nernst-Planck descriptions of electrolyte behavior in the nanopore in the presence of DNA molecules. Two geometries--axially symmetric and cross-sectional--are compared and shown to be compatible. Numerical solutions are carried out on a sufficiently fine mesh to evaluate the viscous drag force acting on DNA inside a silicon nitride nanopore. By assuming the DNA is immobilized in the axial center of the nanopore, the calculation result of this viscous drag force is found to be rather larger than the experimental result. Because the viscous drag force decreases if DNA is closer to the surface of the nanopore, however, the relevant effective driving force is the average over all possible positions of the DNA in the nanopore. When this positional uncertainty is taken into account, the effective driving force acting on DNA inside the nanopore is found to agree very well with the experimental results.
Luminescence Characteristics of YAG Glass–Ceramic Phosphor for White LED (2008)

Luminescence characteristics of Ce:Y <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> Al <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">5</sub> O <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">12</sub> (YAG) glass-ceramic (GC) phosphor for a white LED were investigated. The GC phosphor was obtained by a heat treatment of a Ce-doped SiO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> -Al <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> O <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> -Y <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> O <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> mother glass between 1300degC and 1500degC for the prescribed time period. The quantum efficiency (QE) of Ce <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3+</sup> fluorescence in the GC materials, the color coordinate, and the luminous flux of electroluminescence of LED composite were evaluated with a blue LED (465 nm) set in an integrating sphere. The QE increased with increasing ceramming temperature of the as-made glass. The color coordinates ( <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">x</i> , <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">y</i> ) of the composite were increased with increasing thickness of the GC mounted on a blue LED chip. The effect of Gd <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> O <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> substitution on the optical properties of the GC materials was also investigated. The excitation and emission wavelengths shifted to longer side up to Gd/(Y + Gd) = 0.40 in molar composition. As a result, the color coordinate of the LED with GdYAG-GC of various thickness shifted to closer to the Planckian locus for the blackbody radiation. These results were explained by partial substitution of Gd <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3+</sup> ions in the precipitated YAG microcrystals, leading to the increase of lattice constant of unit cell, which was confirmed by XRD.
Histopathology of Embolic Debris Captured During Transcatheter Aortic Valve Replacement (2013)

Embolic debris traveling to the brain was captured in 75% of transcatheter aortic valve replacement procedures where a filter-based embolic protection device was used. The debris consisted of fibrin, or amorphous calcium and connective tissue derived most likely from either the native aortic valve leaflets or aortic wall.