1140 IDENTIFICATION OF OCCULT LYMPH NODE DISEMINATION IN PATIENTS WITH BLADDER CANCER USING FORMALIN-FIXED PARAFFIN-EMBEDDED SAMPLES (2010)

You have accessJournal of UrologyBladder Cancer: Basic Research III1 Apr 20101140 IDENTIFICATION OF OCCULT LYMPH NODE DISEMINATION IN PATIENTS WITH BLADDER CANCER USING FORMALIN-FIXED PARAFFIN-EMBEDDED SAMPLES Cristina Gazquez, Lourdes Mengual, Maria J. Ribal, Mercedes Marin-Aguilera, Pedro L. Fernandez, and Antonio Alcaraz Cristina GazquezCristina Gazquez More articles by this author , Lourdes MengualLourdes Mengual More articles by this author , Maria J. RibalMaria J. Ribal More articles by this author , Mercedes Marin-AguileraMercedes Marin-Aguilera More articles by this author , Pedro L. FernandezPedro L. Fernandez More articles by this author , and Antonio AlcarazAntonio Alcaraz More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2010.02.639AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES The aim of this study was to test two potential mRNA markers of bladder urothelial cell carcinoma (UCC) in lymph nodes (LN) and to compare the performance of these markers with patients' clinical outcome. METHODS A total of 189 and 35 formalin-fixed paraffin-embedded (FFPE) LN samples from 90 UCC patients and 35 controls, respectively, were collected from 1993 to 2005 (median follow-up of 100 months). According to previous results of our group, two genes, FXYD3 and KRT20, were selected to study UCC dissemination in LN. RNA was extracted from 3 to 16-year-old FFPE LN samples. Total RNA was reverse transcribed, preamplified and finally evaluated by quantitative real time PCR (qRT-PCR). The resulting gene expression values were statistically compared with histological results and patients' clinical outcome. RESULTS We found that the combination of FXYD3 and KRT20 genes yielded a 100% sensitivity and specificity differentiating LN with UCC dissemination from controls. The expression of both genes allowed the identification of UCC in 5.7% of patients with previous histologically negative LN. Half of these patients died because of the bladder cancer. CONCLUSIONS Using the combination of two molecular markers it was possible to improve the sensitivity of histological analysis to detect occult LN dissemination. Even though half of the histologically negative and qRT-PCR positive LN patients did not recurred after a median follow-up of 100 months, it remains possible that lymphadenectomy had a curative effect in these patients. Thus, the analysis of these two molecular markers could be a complementary technique to the routine histological analysis. Barcelona, Spain© 2010 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 183Issue 4SApril 2010Page: e441 Advertisement Copyright & Permissions© 2010 by American Urological Association Education and Research, Inc.Metrics Author Information Cristina Gazquez More articles by this author Lourdes Mengual More articles by this author Maria J. Ribal More articles by this author Mercedes Marin-Aguilera More articles by this author Pedro L. Fernandez More articles by this author Antonio Alcaraz More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...