gebhart/patrepeval huggingface dataset:
Characterization and structural modeling of a novel thermostable glycine oxidase from <i>Geobacillus kaustophilus</i> HTA426 (2007)

Glycine oxidase from Geobacillus kaustophilus HTA426 (GOXK) is a 43 kDa monomer flavoenzyme containing noncovalently bound FAD. The induction of the enzyme resulted in the expression of a fully soluble protein with higher specific activity than those previously reported for GOX from B. subtilis (GOXB). A study of the kinetic properties of this novel GOXK revealed the lowest KM values for most of the substrates analyzed, with the exception of D-proline which kept a similar value and had the highest Vmax value reported. The Vmax/KM ratio maintained a substrate preference of GOXK for amines of small size, like glycine, sarcosine, N-ethyl-glycine, and glycine-ethyl-ester. GOXK presented good stability at 60-70 degrees C and in alkaline media (pH 6-9.5). The putative tridimensional structure was modeled by sequence alignment and by comparing the changes between GOXK and GOXB, and the residues that could be responsible for the substrate specificity as well as those essential for the catalytic activity were found. The comparison between the possible topology of GOXK with that of GOXB showed changes at the putative interactions between monomers for the building of the tetrameric oligomerization.
Proposals for the description and measurement of carry-over effects in clinical chemistry (Recommendations 1991) (1991)

Abstract
Integrated transportation and energy activity-based model (2010)

Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Civil and Environmental Engineering, 2010.
You're capped (2012)

Bandwidth caps, a limit on the amount of data users can upload and download in a month, are common globally for both home and mobile Internet access. With caps, each bit of data consumed comes at a cost against a monthly quota or a running tab. Yet, relatively little work has considered the implications of this usage-based pricing model on the user experience. In this paper, we present results from a qualitative study of households living with bandwidth caps. Our findings suggest home users grapple with three uncertainties regarding their bandwidth usage: invisible balances, mysterious processes, and multiple users. We discuss how these uncertainties impact their usage and describe the potential for better tools to help monitor and manage data caps. We conclude that as a community we need to cater for users under Internet cost constraints.
A tyrosine kinase associated with the red cell membrane phosphorylates band 3. (1983)

Phosphorylation of Band 3, the anion transport protein of human erythrocyte membranes, has been studied by incubating isolated ghosts with [gamma-32P]ATP. One of the phosphate-acceptor sites is tyrosine 8 in the NH2-terminal cytoplasmic domain of the Band 3 protein. Seven out of 11 residues in the sequence surrounding the phosphorylated tyrosine are Asp or Glu. It is concluded that the erythrocyte, like other cells, contains a membrane-associated tyrosine kinase which phosphorylates highly anionic peptide acceptor sites.
Robust test automation using contextual clues (2014)

Despite the seemingly obvious advantage of test automation, significant skepticism exists in the industry regarding its cost-benefit tradeoffs. Test scripts for web applications are fragile: even small changes in the page layout can break a number of tests, requiring the expense of re-automating them. Moreover, a test script created for one browser cannot be relied upon to run on a different web browser: it requires duplicate effort to create and maintain versions of tests for a variety of browsers. Because of these hidden costs, organizations often fall back to manual testing.
Predictors of fetal hemoglobin response in children with sickle cell anemia receiving hydroxyurea therapy (2002)

In the phase I/II pediatric hydroxyurea safety trial (HUG-KIDS), school-aged children with sickle cell anemia receiving hydroxyurea at the maximally tolerated dose (MTD) had variable increases in the percentage of fetal hemoglobin (%HbF). To identify predictors of the HbF response to hydroxyurea therapy, baseline clinical and laboratory values (age, sex, hemoglobin concentration, %HbF, reticulocytes, white blood cell [WBC], platelets, and serum chemistries), as well as treatment variables (number of toxicities, noncompliance, MTD dose, and MTD blood counts) were analyzed in 53 HUG-KIDS children who achieved MTD. Baseline %HbF values (P =.001), baseline hemoglobin concentration (P =.01), MTD dose (P =.02), and compliance (P =.02) were significantly associated with a higher %HbF at MTD; in contrast, age, sex, number of toxicities, and other baseline hematologic parameters were not. After adjusting for variations in baseline %HbF, the baseline reticulocyte count (P =.05) and baseline WBC count (P =.05) were also significantly associated with a higher %HbF at MTD. Hydroxyurea-induced increases in the hemoglobin concentration and mean corpuscular volume (both higher absolute values at MTD and larger positive changes from baseline values), as well as hydroxyurea-induced decreases in reticulocytes and WBC count, were significantly associated with a higher %HbF at MTD. These data suggest that selected baseline laboratory parameters, a higher MTD dose with attention to compliance, and greater therapy-related changes in blood counts may predict the HbF response to hydroxyurea therapy for children with sickle cell anemia. The HbF response to hydroxyurea is variable and complex, however, and even children with low baseline %HbF values can develop substantial increases in %HbF at MTD.
Expression of SULTR2;2, encoding a low-affinity sulphur transporter, in the Arabidopsis bundle sheath and vein cells is mediated by a positive regulator (2018)

The bundle sheath provides a conduit linking veins and mesophyll cells. In the C3 plant Arabidopsis thaliana, it also plays important roles in oxidative stress and sulphur metabolism. However, the mechanisms responsible for the patterns of gene expression that underpin these metabolic specializations are poorly understood. Here, we used the Arabidopsis SULTR2;2 gene as a model to better understand mechanisms that restrict expression to the bundle sheath. Deletion analysis indicated that the SULTR2;2 promoter contains a short region necessary for expression in the bundle sheath and veins. This sequence acts as a positive regulator and is tolerant to multiple consecutive deletions indicating considerable redundancy in the cis-elements involved. It is highly conserved in SULTR2;2 genes of the Brassicaceae and is functional in the distantly related C4 species Flaveria bidentis that belongs to the Asteraceae. We conclude that expression of SULTR2;2 in the bundle sheath and veins is underpinned by a highly redundant sequence that likely represents an ancient and conserved mechanism found in families as diverse as the Asteraceae and Brassicaceae.
The idiopathic hypereosinophilic syndrome (1994)

Abstract THE IDIOPATHIC hypereosinophilic syndrome (HES) is a leukoproliferative disorder, or more likely disorders, marked by a sustained overproduction of eosinophils. The distinctiveness of the syndrome, in addition to its eosino-philia, is its marked predilection to damage specific organs, including the heart. Such cardiac pathology is not unique to the idiopathic HES, because it may develop with eosinophilia associated with other diseases with identifiable etiologies. Conversely, yet enigmatically, not all patients with hypereo-sinophilia develop the organ damage characteristic of the HES. There are no specific tests diagnostic of the HES rather, the syndrome is defined by the combination of unexplained prolonged eosinophilia and evidence of organ involvement. We first review the more common and variable hematologic and clinical manifestations of this disorder because these are the features encountered clinically and that must be explained by investigation of the pathogenesis of HES. After considering the current understanding of the etiology and pathogenesis of the manifestations of HES, we review the therapies that may be used for HES.
T.E. Lawrence and the English Patient (2006)

The life of T.E. Lawrence and his epic account of Arabian campaign, Seven Pillars of Wisdom (1926), though not mentioned acknowledgments, powerful influence on Michael Ondaatje's The English Patient (1992). Both Lawrence and Ladislaus de Almasy (the eponymous hero) are aristocrats: Lawrence illegitimate son of Sir Thomas Chapman, Almasy Hungarian count. Like Lawrence, Almasy is well read and learned, and has great deal of specialized technical knowledge. Both love Histories of Herodotus. In 1930 Lawrence wrote that is marvelous fellow.... Also he is preeminently human. A first-rate Herodotus would be most useful book (The Letters of T.E. Lawrence, ed. David Garnett, 1938; London: Spring Books, 1964, p. 681). Almasy carries precious copy of Herodotus with him throughout war, and enhances it with other works: in his commonplace book, his 1890 edition of Herodotus' Histories, are other fragments-maps, diary entries, writings many languages, paragraphs cut out of other (Ondaatje, The English Patient, NY: Vintage, 1993, p. 96). Like Lawrence, Almasy was a lover.... He spent his childhood [as Lawrence spent his young adult life] Levant and knew Bedouin (p. 165). Before and during World War I Lawrence went east from Cairo to explore and fight Arabian desert; before and during World War II Almasy went west from Cairo to explore and fight Libyan desert. Both men wrote about their explorations: Lawrence Crusader Castles, Carchemish and The Wilderness of Zin; Almasy scholarly papers and monographs. Both had written books about ... disappearance and reappearance of oases, lost culture of deserts (p. 143). Both used archeology to disguise their spying. Ondaatje seems to be speaking of Lawrence as well as of Almasy when he observes: Some of English love Africa [or Arabia]. A part of their brain reflects precisely. So they're not foreigners (p. 33). Lawrence, mentioning Bedouins who attained heights and depths beyond our reach, though not beyond our sight and identifying with these people, maintained that they realized our absolute their unrestrained capacity for good and evil (Lawrence, Seven Pillars of Wisdom, Garden City, NY: Doubleday, 1935, p. 586). In lyrical passage about ruined yet still fragrant Roman palace desert, Lawrence wrote: at last Dahoum drew me: 'Come and smell very sweetest scent of all,' and we went into main lodging, to gaping window sockets of its eastern face, and there drank with open mouths of effortless, empty, eddyless wind of desert (Seven Pillars, p. 40). Ondaatje, mode that recalls Lawrence's style, writes that the could not be claimed or owned--it was piece of cloth carried by winds (p. 138). Ondaatje attributes Lawrence's military skills to both Almasy and to Sikh sapper, Kirpal Singh. Like Lawrence, who blew up many trains on Hejaz railway, Kip (as he's called) is an expert explosives and demolitions. In July 1917 Lawrence captured Akaba on Red Sea after what was thought to be an impossible journey, torturous six-hundred-mile ride through and descent from interior to unguarded eastern side of town. …
Kinetics of ozonation. 6. Polycyclic aliphatic hydrocarbons (1988)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTKinetics of ozonation. 6. Polycyclic aliphatic hydrocarbonsDavid H. Giamalva, Daniel F. Church, and William A. PryorCite this: J. Org. Chem. 1988, 53, 15, 3429–3432Publication Date (Print):July 1, 1988Publication History Published online1 May 2002Published inissue 1 July 1988https://pubs.acs.org/doi/10.1021/jo00250a005https://doi.org/10.1021/jo00250a005research-articleACS PublicationsRequest reuse permissionsArticle Views184Altmetric-Citations31LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Corrosion of Steel by Lead Bismuth Eutectic: Quarterly Report November- December 2002 January 2003 (2003)

We continue to get valuable data from sputter depth profiling of steel samples. We meet weekly to discuss progress. We took depth-profiling data on D9 steel that had been exposed to LBE. This the first time we have examined this type of steel. This is important because it will enable us to separate out two effects and determine their effect on corrosion: composition of the steel vs surface preparation.\nWe started to plan a small experiments using a crucible of heated LBE, with the research program to be conducted at UNLV, on basic aspects of corrosion in this system. The idea is to conduct small-scale experiments to corrode steel samples using lead-bismuth eutectic. These experiments would be conducted using small "pots" of molten LBE, not the massive LBE loop that UNLV now has. A proposal was submitted at the end of February 2003. While that was not funded because of budget constraints, we hope to make a start on such a program on a much-reduced budget.
Influence of Surfactants and Dissolved Gases on the Silver Nanoparticle Plasmon Resonance Absorption Spectra Formed by the Laser Ablation Processes (2013)

The silver nanoparticles were fabricated by the focused laser irradiation to silver rod immersed in various kinds of surfactant aqueous solutions. It was found that anionic and cationic surfactants showed different roles on the silver nanoparticle growth during the focused laser irradiation processes. Silver nanoparticle synthesis in an amphoteric surfactant aqueous solution was also carried out using the same techniques, and it was found that the spectral shifts for these surface plasmon bands showed complicated behaviors against the concentration of amphoteric surfactants as well as pH. Furthermore, the influence of the gas dissolved in a solution on the silver nanoparticle growth during the focused laser irradiation processes was investigated. With increasing the gas pressure of CO 2 , the surface plasmon bands of silver nanoparticles were shifted to longer wavelength, suggesting that the dissolved gas of CO 2 in a solution enhances the silver nanoparticle growth. The plausible mechanism was proposed to understand the reason of such enhancement of silver nanoparticle growth by increasing the dissolved gas in a solution.
Recognition site of <i>Escherichia coli</i> B restriction enzyme on ϕXsB1 and simian virus 40 DNAs: An interrupted sequence (1978)

Methyl groups placed on varphiXsB1 replicative form DNA by the Escherichia coli B modification enzyme are located in the overlap between fragments Mbo II-3 and Alu I-2, a 61-base-pair DNA segment. Mutations that led to loss of susceptibility to restriction by E. coli B occurred within this segment at three positions spanning 14 nucleotides. A sequence difference between varphiXsB1 and varphiXam3cs70, a varphiX174 strain not restricted by E. coli B, occurs at one of these positions. The site on simian virus 40 DNA methylated by the modification enzyme is located in the 115-base-pair overlap between fragments Hae III-I and Alu I-G. The sequences of these segments of varphiXsB1 and simian virus 40 DNA and two regions of phage f1 DNA recognized by the E. coli B restriction enzyme [Ravetch, J. V., Horiuchi, K. & Zinder, N. D. (1978) Proc. Natl. Acad. Sci. USA 75, 2266-2270] contain a homology of nine bases in the configuration:5'-T-G-A... 8N... T-G-C-T... 9N... T-N-N-T-3'. The sequence 5'-T-G-A... 8N... T-G-C-T-3' may constitute the restriction enzyme recognition site since it does not occur in varphiXam3cs70 DNA and occurs only once in simian virus 40 DNA, and since all observed mutations leading to loss of the site occur at one of the bases specified by this sequence. Analysis of the sequence of varphiXam3cs70 showed that if no other residues are recognized, all seven of these bases are essential for recognition and the interval between the two groups of specified bases must be precisely eight.
On the Nonlinear Theory for Gravity Waves on the Ocean's Surface. Part I: Derivations (1977)

A general hydrodynamic solution is derived for arbitrary gravity-wave fields on the ocean surface by extending Stokes' (1847) original perturbational analysis. The solution to the nonlinear equations of motion is made possible by assuming that the surface height is periodic in both space and time and thus can be described by a Fourier series. The assumption of periodicity does not limit the generality of the result because the series can be made to approach an integral representation by taking arbitrarily large fundamental periods with respect to periods of the dominant ocean waves actually present on the surface. The observation areas and times over which this analysis applies are assumed small, however, compared to the periods required for energy exchange processes; hence an "energy balance" (or steady-state) condition is assumed to exist within the observed space-time intervals. This in turn implies the condition of statistical stationarity of the Fourier height coefficients when one generalizes to a random surface. Part I confines itself to the formulation of a perturbation solution (valid to all orders) for the higher order terms resulting from a two-dimensional arbitrary periodic description of the surface height. The method is demonstrated by deriving (to second order) the height correction to the sea and (to third order) the first nonzero correction to the lowest order gravity-wave dispersion relation.
DDDP-a Distributed Data Driven Processor (1983)

This paper describes an architecture of a data flow computer named the Distributed Data Driven Processor (DDDP), and presents an experimental system and the results of experiments using several benchmarks. The experimental system has four processing elements connected by a ring bus, and a structured data memory. The main features of our system are that each processing element is provided with a hardware hashing mechanism to implement token coloring, and a ring bus is used to pass tokens concurrently among processing elements. A hardware monitor was used to measure the performance of the experimental system. The experimental system adopts a low key technology and yet is capable of executing about 0.7 million instructions per second through the benchmarks. This implies that data flow computers can be alternative to the conventional von-Neumann computers if state-of-the-art technologies are adequately introduced.
Noninvasive indirect imaging of vascular endothelial growth factor gene expression using bioluminescence imaging in living transgenic mice (2006)

Vascular endothelial growth factor (VEGF) plays a critical role in the early activation of stromal tissues during wound healing and tumor growth. We report the use of a two-step transcriptional amplification (TSTA) approach to augment the transcriptional activity of the relatively weak VEGF promoter (pVEGF) using firefly luciferase (fl) reporter gene and bioluminescence imaging (BLI). In cell culture, we demonstrate that TSTA-based fl gene expression can be significantly enhanced over the direct one-step system. Using a transgenic mouse model (pVEGF-TSTA-fl), we demonstrate the induction of VEGF gene expression using a wound-healing model and a subcutaneous mammary tumor model. In skin-wounding experiments, pVEGF-induced fl expression in the wound lesion is detected on days 4 and 5 and peaks on days 15-22. Furthermore, the bioluminescence signal shows good correlation with the endogenous VEGF protein levels in the wound tissue (r2 = 0.70). In the mammary tumor model, fl expression is detected on day 3, peaks at day 17, and declines thereafter. These results support the use of noninvasive BLI for the longitudinal monitoring of VEGF induction during wound healing and tumor progression, and this mouse model should find use in various applications in which it is important to noninvasively study VEGF gene expression.
BF<sub>3</sub>·OEt<sub>2</sub> in alcoholic media, an efficient initiator in the cationic polymerization of phenyl‐1,3‐benzoxazines (2013)

ABSTRACT 3‐Phenyl‐3,4‐dihydro‐2 H ‐1,3‐benzoxazine ( m 1 ) underwent cationic ring opening polymerization using BF 3 ·OEt 2 in alcoholic solution under mild conditions. The polymerization of m 1 proceeds through an intermediate hemiaminal ether leading mainly to the formation of polybenzoxazines with diphenylmethane bridges, and not only the classical Mannich‐type ones. During the first stages of the reaction, low‐molecular weight soluble oligomers containing benzoxazine rings are formed. At longer polymerization times, the propagation proceeds conventionally through the phenolic active sites. This polymerization mechanism is extensible to other substituted 3‐phenyl‐3,4‐dihydro‐2 H ‐1,3‐benzoxazines but fails in the case of 3‐alkyl‐3,4‐dihydro‐2 H ‐1,3‐benzoxazines or when the phenyl group in Position 3 have a substituent in the p ‐position. Spectroscopic studies and kinetic experiments using model reactions and deuterium labeled benzoxazines, allow proposing a plausible different polymerization mechanism. These soluble benzoxazine‐containing polymers can be conveniently processed and impregnated on appropriate substrates before underwent crosslinking producing materials with comparable properties to those of conventional bis ‐benzoxazines. © 2013 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2013 , 51 , 5075–5084
MBARI's buoy based seafloor observatory design (2005)

There has been considerable discussion and planning in the oceanographic community toward the installation of long-term seafloor sites for scientific observation in the deep ocean. The Monterey Bay Aquarium Research Institute (MBARI) has designed a portable mooring system for deep ocean deployment that provides data and power connections to both seafloor and ocean surface instruments. The surface mooring collects solar and wind energy for powering instruments and transmits data to shore-side researchers using a satellite communications modem. A specialty anchor cable connects the surface mooring to a network of benthic instrumentation, providing the required data and power transfer. Design details and results of laboratory and field testing of the completed portions of the observatory system are described