gebhart/patrepeval huggingface dataset:
Structural characterization of the lipid A component of <i>Bacteroides fragilis</i> strain NCTC 9343 lipopolysaccharide (1989)

The chemical structure of Bacteroides fragilis NCTC 9343 lipid A was characterized by using conventional chemical procedures, methylation analysis, and laser desorption mass spectrometry. It was found that B. fragilis lipid A consists of a beta-D-glucosaminyl-(1-6)-D-glucosaminyl-1-O-phosphate backbone whose hydroxyl groups in positions 4, 4' and 6' are free, the latter serving as the attachment site for the polysaccharide component in lipopolysaccharide. This backbone molecule carries up to of five molecules of ester- and amide-bound long chain non-hydroxylated and (R)-3-hydroxy fatty acids. With regard to the distribution on the fatty acids on the lipid A backbone, a considerable heterogeneity was revealed by laser desorption mass spectrometry. Despite this heterogeneity, a major species of B. fragilis lipid A could be defined in which the hydroxyl group at position 3' of the distal GlcN carries (R)-3-hydroxyhexadecanoic acid and the hydroxyl group at position 3 of the reducing GlcN is acylated by (R)-3-hydroxypentadecanoic acid. The amino group of the distal GlcN residue carries (R)-3-(13-methyltetradecanoyloxy)-15-methylhexadecanoic acid and that of the reducing GlcN group (R)-3-hydroxyhexadecanoic acid. The absence of ester-bound phosphate and ester-linked 3-acyloxyacyl groups, the presence of not more than five acyl residues and the predominance of fatty acids possessing 15-17 carbon atoms are unique features of B. fragilis lipid A which differentiate it from enterobacterial and other lipids A and which are likely to be related to its low endotoxic activity.
Deep Learning of Robotic Tasks without a Simulator using Strong and Weak Human Supervision (2016)

We propose a scheme for training a computerized agent to perform complex human tasks such as highway steering. The scheme is designed to follow a natural learning process whereby a human instructor teaches a computerized trainee. The learning process consists of five elements: (i) unsupervised feature learning; (ii) supervised imitation learning; (iii) supervised reward induction; (iv) supervised safety module construction; and (v) reinforcement learning. We implemented the last four elements of the scheme using deep convolutional networks and applied it to successfully create a computerized agent capable of autonomous highway steering over the well-known racing game Assetto Corsa. We demonstrate that the use of the last four elements is essential to effectively carry out the steering task using vision alone, without access to a driving simulator internals, and operating in wall-clock time. This is made possible also through the introduction of a safety network, a novel way for preventing the agent from performing catastrophic mistakes during the reinforcement learning stage.
Zero-Shot Learning of Text Adventure Games with Sentence-Level Semantics (2020)

Reinforcement learning algorithms such as Q-learning have shown great promise in training models to learn the optimal action to take for a given system state; a goal in applications with an exploratory or adversarial nature such as task-oriented dialogues or games. However, models that do not have direct access to their state are harder to train; when the only state access is via the medium of language, this can be particularly pronounced. We introduce a new model amenable to deep Q-learning that incorporates a Siamese neural network architecture and a novel refactoring of the Q-value function in order to better represent system state given its approximation over a language channel. We evaluate the model in the context of zero-shot text-based adventure game learning. Extrinsically, our model reaches the baseline's convergence performance point needing only 15% of its iterations, reaches a convergence performance point 15% higher than the baseline's, and is able to play unseen, unrelated games with no fine-tuning. We probe our new model's representation space to determine that intrinsically, this is due to the appropriate clustering of different linguistic mediation into the same state.
Optimization Design and Fabrication of Si/SiGe PMOSFETs (2001)

Through the theoretical analysis and computer simulation,the optimized design principles for Si/SiGe PMOSFETs are given,including the choice of gate materials,the determination of Ge percentage and the profile in SiGe channel,the thickness optimization of dioxide and silicon cap layer,and the adjustment of threshold voltage.In light of them,a SiGe PMOSFET is designed and fabricated successfully.The measurements indicate that the transconductance is 45mS/mm (300K) and 92mS/mm (77K) for SiGe PMOSFET's (L=2μm),while it is 33mS/mm (300K) and 39mS/mm (77K) for Si PMOSFET.
Security analysis of a blockchain-based protocol for the certification of academic credentials (2019)

We consider a blockchain-based protocol for the certification of academic credentials named Blockcerts, which is currently used worldwide for validating digital certificates of competence compliant with the Open Badges standard. We study the certification steps that are performed by the Blockcerts protocol to validate a certificate, and find that they are vulnerable to a certain type of impersonation attacks. More in detail, authentication of the issuing institution is performed by retrieving an unauthenticated issuer profile online, and comparing some data reported there with those included in the issued certificate. We show that, by fabricating a fake issuer profile and generating a suitably altered certificate, an attacker is able to impersonate a legitimate issuer and can produce certificates that cannot be distinguished from originals by the Blockcerts validation procedure. We also propose some possible countermeasures against an attack of this type, which require the use of a classic public key infrastructure or a decentralized identity system integrated with the Blockcerts protocol.
Flight dynamics analysis and control of transport aircraft subject to failure (2013)

After a structural damage or component failure during any flight mode, aircraft dynamics are dramatically altered. A quick and adequate stabilization effort is crucial. Flight dynamics for several failure scenarios are analyzed. Necessary amounts of control deflections for postfailure trim are calculated. These trim values are used as control input in an open loop manner and validity of this approach is tested via flight simulations. Alternatively, a closed loop flight control system, which does not need the postfailure trim values, is also designed. This closed loop controller is based on a linearized aircraft model whereas flight simulations are based on nonlinear aircraft dynamics.
SED1/MFG‐E8: A Bi‐Motif protein that orchestrates diverse cellular interactions (2009)

MFG-E8 was initially identified as a principle component of the Milk Fat Globule, a membrane-encased collection of proteins and triglycerides that bud from the apical surface of mammary epithelia during lactation. It has since been independently identified in many species and by many investigators and given a variety of names, including p47, lactadherin, rAGS, PAS6/7, and BA-46. The acronym SED1 was proposed to bring cohesion to this nomenclature based upon it being a Secreted protein that contains two distinct functional domains: an N-terminal domain with two EGF-repeats, the second of which has an integrin-binding RGD motif, and a C-terminal domain with two Discoidin/F5/8C domains that bind to anionic phospholipids and/or extracellular matrices. SED1/MFG-E8 is now known to participate in a wide variety of cellular interactions, including phagocytosis of apoptotic lymphocytes and other apoptotic cells, adhesion between sperm and the egg coat, repair of intestinal mucosa, mammary gland branching morphogenesis, angiogenesis, among others. This article will explore the various roles proposed for SED1/MFG-E8, as well as its provocative therapeutic potential.
Demonstration that lysine-501 of the .alpha. polypeptide of native sodium and potassium ion activated adenosine triphosphatase is located on its cytoplasmic surface (1987)

Evidence that the peptide HLLVMKGAPER, which can be released from intact sodium and potassium ion activated adenosinetriphosphatase by tryptic digestion, is located on the cytoplasmic surface of the native enzyme has been obtained. An immunoadsorbent directed against the carboxy-terminal sequence of this tryptic peptide has been constructed. The peptide KGAPER was synthesized by solid-phase techniques. Antibodies against the sequence -GAPER were purified by immunoadsorption, using the synthetic peptide attached to agarose beads. These antibodies, in turn, were coupled to agarose beads to produce an immunoadsorbent. Sealed, right-side-out vesicles, prepared from canine kidneys, were labeled with pyridoxal phosphate and sodium [3H]borohydride in the absence or presence of saponin, respectively. A tryptic digest of these labeled vesicles was passed over the immunoadsorbent. Large increases in the incorporation of radioactivity into the peptides bound by the immunoadsorbent were observed in the digests obtained from the vesicles exposed to saponin. From the results of several control experiments examining the labeling reaction as applied to these vesicles, it could be concluded that this increase in incorporation resulted only from the access that the reagents gained to the inside of the vesicles in the presence of saponin and that the increase in the extent of modification was due to the cytoplasmic disposition of this segment in the native enzyme.
An Economic Analysis of Pension Tax Proposals (2015)

The Government has recently issued a consultation document which raises the possibility of a substantial change in the taxation of pensions. In this paper we assess the economic consequences of changing from the existing EET system (where pension savings and returns are exempt from income tax, but pension income is taxed) to a TEE system (pension savings would be from taxed income but with no further taxation thereafter), making use of two complementary approaches. First, we review the economic and empirical literature, and second we construct a general equilibrium overlapping generations (OLG) model parameterised to UK data and the UK tax system.
Inhibition of HERG K<sup>+</sup> Current and Prolongation of the Guinea‐Pig Ventricular Action Potential by 4‐Aminopyridine (2003)

4-Aminopyridine (4-AP) has been used extensively to study transient outward K+ current (ITO,1) in cardiac cells and tissues. We report here inhibition by 4-AP of HERG (the human ether-à-go-go-related gene) K+ channels expressed in a mammalian cell line, at concentrations relevant to those used to study ITO,1. Under voltage clamp, whole cell HERG current (IHERG) tails following commands to +30 mV were blocked with an IC50 of 4.4 +/- 0.5 mM. Development of block was contingent upon HERG channel gating, with a preference for activated over inactivated channels. Treatment with 5 mM 4-AP inhibited peak IHERG during an applied action potential clamp waveform by ~59 %. It also significantly prolonged action potentials and inhibited resurgent IK tails from guinea-pig isolated ventricular myocytes, which lack an ITO,1. We conclude that by blocking the alpha-subunit of the IKr channel, millimolar concentrations of 4-AP can modulate ventricular repolarisation independently of any action on ITO,1.
In vitro and in vivo protective effects of proteoglycan isolated from mycelia of Ganoderma lucidum on carbon tetrachloride-induced liver injury (2006)

GLPG has hepatic protective activity against CCl4-induced injury both in vitro and in vivo. The possible anti-hepatotoxic mechanisms may be related to the suppression of TNF-alpha level and the free radical scavenging activity.
Autocrine TGF-β Induces Epithelial to Mesenchymal Transition in Human Amniotic Epithelial Cells (2013)

Human amniotic epithelial cells (hAECs) have been the object of intense research due to their potential therapeutic use. In this paper, we present molecular evidence of a bona fide epithelial to mesenchymal transition (EMT) undergone by hAECs. Amniotic membrane (AM)-derived hAECs showed the presence of typical epithelial markers such as E-cadherin and cytokeratins. hAECs in culture, however, underwent morphological changes acquiring a mesenchymal shape. Epithelial cell markers were lost and typical mesenchymal markers, such as vimentin and α-SMA, appeared. Several genes associated with EMT, such as SNAI1, MMP9, PAI1, or ACTA2, increased their expression. The expression of the transcription activators KLF4 or MTA3 was consistent with the downregulation of CDH1. We have shown that hAECs undergo EMT due to the autocrine production of TGF-β. Furthermore, the addition of the TGF-β receptor I (ALK5) inhibitor SB-431542 or TGF-β neutralizing antibody to hAECs prevented EMT and preserved the hAECs' epithelial phenotype. Altogether, these results suggest that cultured hAECs undergo EMT through the autocrine production of TGF-β.
SDN enabled content distribution in vehicular networks (2014)

With the rapid advances of cellular and DSRC communication technologies, vehicle to vehicle (V2V) and vehicle to infrastructure (V2I) communication will greatly improve the safety and mobility of future transportation systems. Nevertheless, current TCP/IP based networking services are too rigid to support large scale bandwidth-intensive content distribution. We focus on two challenging applications: distribution of real-time traffic information to all road vehicles and distribution of bandwidth-intensive media data. In this paper, we propose a Type-Based Content Distribution (TBCD) approach to support large scale data intensive content distribution in vehicular networks. It utilizes the Software-Defined Networking (SDN) technology to support efficient in-network replication and caching. According to the different types of the content, our method takes a push-and-pull pattern to transmit the content. Also, we take different policies based on the number of subscribers to utilize the push-and-pull pattern more efficiently. It aims to achieve the following points: (1) to balance reliability and redundancy of the content forwarding, and (2) to minimize the impact of disconnection when transmitting the content in a vehicular wireless network.
Rapid Release of Antipsychotic Drugs From Dopamine D<sub>2</sub> Receptors: An Explanation for Low Receptor Occupancy and Early Clinical Relapse Upon Withdrawal of Clozapine or Quetiapine (1999)

Clozapine and quetiapine are loosely bound to the D2 receptor, and the injected radioactive ligand at its peak concentration may displace some of the D2-bound antipsychotic drug, resulting in apparently low D2 occupancies. Therefore, under clinical brain imaging conditions with [11C]raclopride, D2 occupancies by clozapine and by quetiapine may be higher than currently estimated. These considerations may result in high levels of the D2 receptors being occupied by therapeutic doses of clozapine or quetiapine. The rapid release of clozapine and quetiapine from D2 receptors by endogenous dopamine may contribute to low D2 receptor occupancy and to early clinical relapse upon withdrawal of these medications.
Immunohistochemical analysis of Bcl-2 family proteins in adenocarcinomas of the stomach. (1996)

The apoptosis-regulating proteins Bcl-2, Bax, Bcl-X, Bak, and Mcl-1 were examined by immunohistochemical methods in 48 archival specimens of adenocarcinoma of the stomach, and the results were correlated with tumor histology (intestinal versus diffuse pattern) and clinical stage (early- versus late-stage disease, ie, stages I and II versus stage III). Tumor cells containing immunostaining for the anti-apoptotic proteins Bcl-2, Bcl-X, and Mcl-1 were present in 26 (54%), 41 (85%), and 36 (75%) of the 48 cases evaluated, respectively, whereas immunopositivity for the pro-apoptotic proteins Bax and Bak was found in 44 (92%) and 42 (88%) specimens Comparisons of these immunostaining results with tumor histology revealed statistically significant differences for Bax (P = 0.03), Bcl-X (P = 0.003), and Mcl-1 (P = 0.005), which were all more frequently immunopositive for tumors with an intestinal than a diffuse histological pattern (chi 2 analysis). In addition, the percentage of immunopositive tumor cells was significantly higher for Bcl-X (62 +/- 6% versus 45 +/- 6%, mean +/- SE, P = 0.01) and for Mcl-1 (48 +/- 6% versus 30 +/- 6%; P = 0.04) in tumors with intestinal versus diffuse histology (unpaired t-test). In contrast, the percentage of Bcl-2-immunopositive tumor cells was higher in tumors with diffuse histology compared with intestinal (32 +/- 5% versus 12 +/- 5%; P = 0.01), whereas the percentages of Bax- and Bak-immunopositive tumor cells were not significantly different between these two histological types. In 34 specimens, residual normal gastric epithelial cells (foveolar cells) were present for direct comparisons of immunointensity with tumor cells. The immunointensity for the Bcl-2, Bcl-X, and Mcl-1 proteins was stronger in tumor cells compared with normal foveolar cells in 7 (21%), 15 (44%), and 8 (2.1%) of 34 cases, respectively, whereas the immunointensity of the proapoptotic proteins Bax and Bak was reduced compared with normal cells in 8 (24%) and 24 (71%) cases. Immunointensity, however, did not correlate with histology. clinical stage was not significantly associated with the presence or absence of immunopositive tumor cells, the percentage of immunopositive cells, or immunointensity. Taken together, these results establish for the first time that several Bcl-2 family proteins are expressed in gastric adenocarcinomas and suggest that the repertoire of these proteins may differ depending on the histological type. The findings therefore support the notion that the intestinal and diffuse types of gastric cancer arise at least in part through different mechanisms.
Benchmarking Cellulose Nanocrystals: From the Laboratory to Industrial Production (2016)

The renewability, biocompatibility, and mechanical properties of cellulose nanocrystals (CNCs) have made them an attractive material for numerous composite, biomedical, and rheological applications. However, for CNCs to shift from a laboratory curiosity to commercial applications, researchers must transition from CNCs extracted on the bench scale to material produced on an industrial scale. There are a number of companies currently producing kilogram to ton per day quantities of sulfuric acid-hydrolyzed CNCs as well as other nanocelluloses, as described herein. With the recent intensification of industrially produced CNCs and the variety of cellulose sources, hydrolysis methods, and purification procedures, the characterization of these materials becomes critical. This has further been justified by the past two decades of research that demonstrate that the CNC stability and behavior are highly dependent on the surface chemistry, surface charge density, and particle size. This work outlines key test methods that should be employed to characterize these properties to ensure a "known" starting material and consistent performance. Of the sulfuric acid-extracted CNCs examined, industrially produced material compared well with laboratory-made CNCs, exhibiting similar charge density, colloidal and thermal stability, crystallinity, morphology, and self-assembly behavior. In addition, it was observed that further purification of CNCs using Soxhlet extraction in ethanol had minimal impact on the nanoparticle properties and is unlikely to be necessary for many applications. Overall, the current standing of industrially produced CNCs is positive, suggesting that the evolution to commercial-scale applications will not be hindered by CNC production.
A comparative assessment of two optical fibre techniques for measuring the speed of the electric wind in a negative polarity, atmospheric corona discharge (2006)

Two optical fibre anemometers, one based on a Mach–Zehnder interferometer (OFIA) and another a novel laser-optical fibre Bragg grating sensor (FBGA) have been constructed to measure the speed of the convective air flow generated in the point-plane gap of a negative corona discharge in atmospheric air. In both configurations the sensing section of the optical fibre anemometers is subjected to controlled, repetitive bursts of infrared radiation from a CO2 laser and the combination of localized heating and convective cooling by the corona wind results in an optical signal that is directly calibrated to the speed of the wind. Both the OFIA and FBGA successfully measured the speed of the corona wind in the discharge gap, with wind speeds ranging from 0 to 0.7 m s−1 observed at different locations in the discharge gap. However, the FBGA, due primarily to the ability to average sensor response over many measurements, exhibited an order of magnitude higher sensitivity than the OFIA (Δv ≈ 4 × 10−3 m s−1 compared to 3 × 10−2 m s−1). Both sensors yielded measurements of wind speed that were an order of magnitude lower than similar measurements using laser Doppler anemometry (LDA), indicating that the seed particles introduced as part of the LDA system are providing false reading of wind speed due to acceleration by the gap electric field.
Stereochemical control of microbial reduction. 17. A method for controlling the enantioselectivity of reductions with bakers' yeast (1991)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStereochemical control of microbial reduction. 17. A method for controlling the enantioselectivity of reductions with bakers' yeastKaoru Nakamura, Yasushi Kawai, Nobuyoshi Nakajima, and Atsuyoshi OhnoCite this: J. Org. Chem. 1991, 56, 15, 4778–4783Publication Date (Print):July 1, 1991Publication History Published online1 May 2002Published inissue 1 July 1991https://pubs.acs.org/doi/10.1021/jo00015a038https://doi.org/10.1021/jo00015a038research-articleACS PublicationsRequest reuse permissionsArticle Views494Altmetric-Citations185LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Control Design for an Advanced Geared Turbofan Engine (2017)

This paper describes the design process for the control system of an advanced geared turbofan engine. This process is applied to a simulation that is representative of a 30,000 pound-force thrust class concept engine with two main spools, ultra-high bypass ratio, and a variable area fan nozzle. Control system requirements constrain the non-linear engine model as it operates throughout its flight envelope of sea level to 40,000 feet and from 0 to 0.8 Mach. The purpose of this paper is to review the engine control design process for an advanced turbofan engine configuration. The control architecture selected for this project was developed from literature and reflects a configuration that utilizes a proportional integral controller with sets of limiters that enable the engine to operate safely throughout its flight envelope. Simulation results show the overall system meets performance requirements without exceeding operational limits.