gebhart/patrepeval huggingface dataset:
‘Dopamine‐first’ mechanism enables the rational engineering of the norcoclaurine synthase aldehyde activity profile (2015)

Norcoclaurine synthase (NCS) (EC 4.2.1.78) catalyzes the Pictet-Spengler condensation of dopamine and an aldehyde, forming a substituted (S)-tetrahydroisoquinoline, a pharmaceutically important moiety. This unique activity has led to NCS being used for both in vitro biocatalysis and in vivo recombinant metabolism. Future engineering of NCS activity to enable the synthesis of diverse tetrahydroisoquinolines is dependent on an understanding of the NCS mechanism and kinetics. We assess two proposed mechanisms for NCS activity: (a) one based on the holo X-ray crystal structure and (b) the 'dopamine-first' mechanism based on computational docking. Thalictrum flavum NCS variant activities support the dopamine-first mechanism. Suppression of the non-enzymatic background reaction reveals novel kinetic parameters for NCS, showing it to act with low catalytic efficiency. This kinetic behaviour can account for the ineffectiveness of recombinant NCS in in vivo systems, and also suggests NCS may have an in planta role as a metabolic gatekeeper. The amino acid substitution L76A, situated in the proposed aldehyde binding site, results in the alteration of the enzyme's aldehyde activity profile. This both verifies the dopamine-first mechanism and demonstrates the potential for the rational engineering of NCS activity.
Histology of the rat vas deferens after injection of a non-occlusive chemical contraceptive (1981)

An alternate co-polymer of styrene and maleic anhydride was dissolved in dimethylsulphoxide and injected into the vas deferens of rats. The polymer was retained in the vas deferens and the morphological changes detected were confined to the mucosa. When the polymer was removed by flushing dimethylsulphoxide, the mucosal structure became normal within 2 weeks.
In Silico Identification and Experimental Characterization of Regulatory Elements Controlling the Expression of the Salmonella csrB and csrC Genes (2013)

The small RNAs CsrB and CsrC of Salmonella indirectly control the expression of numerous genes encoding widespread cellular functions, including virulence. The expression of csrB and csrC genes, which are located in different chromosomal regions, is coordinated by positive transcriptional control mediated by the two-component regulatory system BarA/SirA. Here, we identified by computational analysis an 18-bp inverted repeat (IR) sequence located far upstream from the promoter of Salmonella enterica serovar Typhimurium csrB and csrC genes. Deletion analysis and site-directed mutagenesis of the csrB and csrC regulatory regions revealed that this IR sequence is required for transcriptional activation of both genes. Protein-DNA and protein-protein interaction assays showed that the response regulator SirA specifically binds to the IR sequence and provide evidence that SirA acts as a dimer. Interestingly, whereas the IR sequence was essential for the SirA-mediated expression of csrB, our results revealed that SirA controls the expression of csrC not only by binding to the IR sequence but also by an indirect mode involving the Csr system. Additional computational, biochemical, and genetic analyses demonstrated that the integration host factor (IHF) global regulator positively controls the expression of csrB, but not of csrC, by interacting with a sequence located between the promoter and the SirA-binding site. These findings contribute to the better understanding of the regulatory mechanism controlling the expression of CsrB and CsrC.
NON Idling HVAC-module for Long Distance Trucks (1999)

<div class="htmlview paragraph">The climate comfort of the passenger compartment of cars and commercial vehicles can be increased considerably compared to given levels, by an air condition which is independent of the engine. When this type of air condition is used, the interior of cars can be cooled to a comfortable temperature or the air condition can be maintained during short rests. The same is true for commercial vehicles. In this case the cabin is a working area, a resting- and sleeping area at the same time and so in addition non-idling air condition with a longer duration is required. Available systems are discussed and evaluated. The most promising system is the latent cooling battery technology. Prototypes of this system, its technology, requirements, constructions and test results are presented.</div>
Conversion of viable but nonculturable enteric bacteria  to culturable by co‐culture with eukaryotic cells (2012)

Viable but nonculturable (VBNC) Vibrio cholerae non-O1/non-O139, V. parahaemolyticus, enterohemorrhagic Escherichia coli, enterotoxigenic E. coli, enteropathogenic E. coli, Shigella flexneri, and Salmonella enterica were converted to the culturable state by co-culture with selected eukaryotic cells, e.g., HT-29, Caco-2, T84, HeLa, Intestine 407, and CHO cells.
Cyclic RGD-Linked Polymeric Micelles for Targeted Delivery of Platinum Anticancer Drugs to Glioblastoma through the Blood–Brain Tumor Barrier (2013)

Ligand-mediated drug delivery systems have enormous potential for improving the efficacy of cancer treatment. In particular, Arg-Gly-Asp peptides are promising ligand molecules for targeting αvβ3/αvβ5 integrins, which are overexpressed in angiogenic sites and tumors, such as intractable human glioblastoma (U87MG). We here achieved highly efficient drug delivery to U87MG tumors by using a platinum anticancer drug-incorporating polymeric micelle (PM) with cyclic Arg-Gly-Asp (cRGD) ligand molecules. Intravital confocal laser scanning microscopy revealed that the cRGD-linked polymeric micelles (cRGD/m) accumulated rapidly and had high permeability from vessels into the tumor parenchyma compared with the PM having nontargeted ligand, "cyclic-Arg-Ala-Asp" (cRAD). As both cRGD/m- and cRAD-linked polymeric micelles have similar characteristics, including their size, surface charge, and the amount of incorporated drugs, it is likely that the selective and accelerated accumulation of cRGD/m into tumors occurred via an active internalization pathway, possibly transcytosis, thereby producing significant antitumor effects in an orthotopic mouse model of U87MG human glioblastoma.
Comparison of Manual and Automatic Section Positioning of Brain MR Images (2006)

The study protocol was approved by the institutional review board and was in full compliance with HIPAA guidelines. Informed consent was obtained from all patients. The purpose of this study was to prospectively compare intra- and intersubject variability of manual versus automatic magnetic resonance (MR) imaging section prescription. In two examinations, T2-weighted series were acquired with both methods. All intrasubject and three of six intersubject section prescription variances were significantly higher for manual prescription (P < .01). Root mean square errors confirmed better coregistration of the automated approach (P < .001). Automatic section prescription leads to improved reproducibility of imaging orientations for intra- and intersubject series in clinical practice.
3. Seismic Properties of Carbonate Rocks (1997)

The study of seismic reservoir properties is an important aspect of reservoir characterization and management using seismic technology because it directly links seismology to rock properties. Results of such a study, both experimental and theoretical, provide a fundamental understanding of seismic responses to reservoir properties and their dynamic changes so that seismic attributes can be converted to reservoir parameters. Unlike siliceous clastic reservoir rocks, carbonate rocks tend to have complicated pore systems, such as vugs, molds, channels, and fractures. Different types of pores have different compressibilities so they affect seismic wave velocities differently. Furthermore, pore types also control seismic responses to pore fluid changes in the reservoir. Besides pore types, factors that influence seismic wave velocities in carbonate rocks include mineralogy, porosity, pore fluid type and saturation, and reservoir parameters such as pressure and temperature. Studies of seismic properties of carbonate rocks play an important role in seismic reservoir characterization and monitoring. Recent laboratory results show that carbonate pore type and pore fluid compressibility contrast are the two most important factors in the feasibility of seismic monitoring of fluid movement in carbonate reservoirs.
Enantioselective Aziridination Using Copper Complexes of Biaryl Schiff Bases (2002)

Racemic 2,2'-diamino-6,6'-dimethylbiphenyl is resolved using simulated moving bed chromatography, and the absolute configuration of the enantiomers is confirmed via the X-ray crystal structure of a derivative. The diamine is condensed with a range of aldehydes to give bidentate aldimine proligands L. Molecular structures of the complexes formed between L and Cu(I) fall into two classes; bimetallic double helices ([Cu(2)L(2)](2+)) and monometallic ([CuL](+)). The latter are strikingly more efficient in the aziridination of alkenes than are the former in terms of rate, turnover, and enantioselection. In particular, the imine ligand formed from the diamine and 2,6-dichlorobenzaldehyde gives, in combination with Cu(I) or Cu(II), up to 99% ee in the aziridination of 6-acyl-2,2-dimethylchromene and 88-98% ee for a range of cinnamate esters. Styrenic and other alkenes are converted with lower selectivities (5-54%). The catalytic system shows a linear response in product ee to catalyst ee, and the product ee does not vary significantly during the reaction. UV spectrophotometric investigations indicate that conversion of Cu(I) to Cu(II) is not essential for catalysis but that Cu(II) is probably also a competent system.
Está acabando el silencio con su empresa (2003)

Cuando los miembros de una organizacion se enfrentan a problemas organizativos o interpersonales en el trabajo, a menudo prefieren no hablar porque creen que el esfuerzo no vale la pena y que es preferible trabajar como si no sucediera nada. Sin embargo, nuevas investigaciones muestran que el precio del silencio es mucho mayor del que se suele creer. Asi, es necesario expresar las ideas y no guardarse las inquietudes dentro.
Optimal Rate Control for Transporting VBR Video over QoS-assured Channels (2007)

在保证的 QoS (服务的质量) 上在我们讨论怎么为搬运可变小点率(VBR ) 选择适当来源和隧道率的这份报纸压缩了录像隧道。我们首先作为分离线性时间延期系统的一个最佳的控制问题提出它。然后，分离最大的原则被用来得到最佳的控制。比作传统的解决方案，没有特殊要求，建议算法为编码器和解码器缓冲区尺寸与连续输出率，和罐头工作为编码者被设计。理论分析和建议算法举办的试验性的结果表演降低空间和时间复杂性。我们的答案能被使用在离线、联机编码。
Table of Contents (2018)

Conference Proceedings, OCG. 2018. “Table of Contents”. Central and Eastern European EDem and EGov Days 331 (July):7-10. https://doi.org/10.24989/ocg.v331.0.
Flanking Duplications and Deletions Associated With <i>P</i>-Induced Male Recombination in Drosophila (1996)

We studied P element-induced recombination in germline mitotic cells by examining the structure of the recombinant chromosomes. We found that most recombinants retain a mobile P element at the site of the recombination, usually with either a deletion or a duplication immediately adjacent to the P end at which the crossover occurred. The sizes of these deletions and duplications ranged from a few base pairs to well over 100 kb. These structures fit the "hybrid element insertion" (HEI) model of male recombination in which the two P-element copies on sister chromatids combine to form a "hybrid element" whose termini insert into a nearby position on the homologue. The data suggest that P-induced recombination can be used as an efficient means of generating flanking deletions in the vicinity of existing P elements. These deletions are easily screened using distant flanking markers, and they can be chosen to extend in a given direction depending on which reciprocal recombinant type is selected. Furthermore, the retention of a mobile P element allows one to extend the deletion or generate additional variability at the site by subsequent rounds of recombination.
Machine learning in control of functional electrical stimulation for locomotion (2002)

Two machine learning techniques were evaluated for automatic design of a rule-based control of functional electrical stimulation (FES) for locomotion of spinal cord injured humans. The task was to map the relationship between sensory information and the FES-control signal by using off-line supervised training. Signals were recorded using pressure sensors installed in insoles of a patient's shoes and goniometers attached across the joints of the affected leg. The FES-control signal consisted of pulses corresponding to time intervals when the patient pressed on the manual push-button to deliver the stimulation during FES-assisted ambulation. The machine learning techniques evaluated were the adaptive logic network (ALN) and inductive learning algorithm (IL). Results to date suggest that, given the same training data, the IL learned faster than ALN while both performed the test rapidly. The generalization was better with an ALN, especially if past points were used to reflect the time dimension. Both techniques were able to predict future stimulation events. An advantage of ALN was that it can be retrained with new data without losing previously collected knowledge. The advantages of IL were that IL produces explicit and comprehensible trees and that the relative importance of each sensory contribution can be quantified.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Large-area organic distributed feedback laser fabricated by nanoreplica molding and horizontal dipping (2010)

The fabrication of visible wavelength vertically emitting distributed feedback (DFB) lasers with a subwavelength grating fabricated by a replica molding process and an active polymer layer printed by a horizontal dipping process is reported. The combined techniques enable the organic DFB laser to be uniformly fabricated over large surface areas upon a flexible plastic substrate, with an approach that is compatible with roll-based manufacturing. Using a fixed grating period and depth, DFB laser output wavelength is controlled over a 35 nm range through manipulation of the waveguide layer thickness, which is controlled by the speed of the horizontal dipping process. We also demonstrate that the active area of the structure may be photolithographically patterned to create dense arrays of discrete DFB lasers.
Design and Evaluation of an External Control Arm Using Prior Clinical Trials and Real-World Data (2019)

The use of ECT designs in GBM, with adjustments for the clinical profiles of the enrolled patients, should be preferred to single-arm studies with fixed efficacy thresholds extracted from published results on the current standard of care.
Preparation of a polyurethane membrane testosterone sensor and its application using square-wave stripping voltammetry (2022)

A novel electrochemical sensor for testosterone detection has been prepared by the chemical modification of a gold electrode (AuE). For the electrode modification, specific polyurethane (PU) films were synthesized from hexamethylene diisocyanate, olivetol, polyethylene glycol-100 (PEG100) and β-cyclodextrin. The synthesized PUs were investigated as selective films, and were used to coat a AuE surface at different concentrations and thicknesses. The testosterone responses of the modified electrodes were investigated by square-wave voltammetry (SWV). One separated cathodic SWV peak was obtained for testosterone at –0.390 V, with the prepared PU-modified AuE in 0.1 M phosphate buffer (PB) (pH 7.2). The linearity of testosterone responses of the prepared PU modified electrode was obtained over a concentration range of 0.1–1.0 µM (R2 = 0.995). It was observed that the response of the electrode increased regularly and sensitively with increasing testosterone amount. The detection limit, relative standard deviation and sensitivity of modified electrode were found to be approximately 5.69 nM, 1.669 % and 98.331 %, respectively. The PU-modified AuE exhibited good selectivity and a low response time for testosterone. Therefore, the prepared testosterone sensor offers a good alternative for fast and practical testosterone determination in clinical or biomedical studies.
Comparative mapping of aflatoxin pathway gene clusters in Aspergillus parasiticus and Aspergillus flavus (1995)

Aflatoxins are toxic and carcinogenic secondary metabolites produced by the fungi Aspergillus flavus and A. parasiticus. Aflatoxins are synthesized by condensation of acetate units; their synthesis is estimated to involve at least 16 different enzymes. In this study we have shown that at least nine genes involved in the aflatoxin biosynthetic pathway are located within a 60-kb DNA fragment. Four of these genes, nor-1, aflR, ver-1, and omtA (previously named omt-1), have been cloned in A. flavus and A. parasiticus. In addition, five other genes, pksA, uvm8, aad, ord-1, and ord-2 have been recently cloned in A. parasiticus. The pksA, aad, and uvm8 genes exhibit sequence homologies to polyketide synthase, aryl-alcohol dehydrogenase, and fatty acid synthase genes, respectively. The cDNA sequences of ord-1 and ord-2 genes, which may be involved in later steps of aflatoxin biosynthesis, have been determined; the ord-1 gene product exhibits homology to cytochrome P-450-type enzymes. By characterizing the overlapping regions of the DNA inserts in different cosmid and lambda DNA clones, we have determined the order of these aflatoxin pathway genes within this 60-kb DNA region to be pksA, nor-1, uvm8, aflR, aad, ver-1, ord-1, ord-2, and omtA in A. parasiticus and nor-1, aflR, ver-1, ord-1, ord-2, and omtA in A. flavus. The order is related to the order in enzymatic steps required for aflatoxin biosynthesis. The physical distances (in kilobase pairs) and the directions of transcription of these genes have been determined for both aflatoxigenic species.
Purification and Substrate Specificity of <i>endo</i>-Typo Inulinase from <i>Penicillium purpurogenum</i> (1988)

Journal Article Purification and Substrate Specificity of endo-Typo Inulinase from Penicillium purpurogenum Get access Shuichi Onodera, Shuichi Onodera Department of Food Science, Faculty of Dairy Science, Rakuno Gakuen University, Ebetsu, Hokkaido 069, Japan Search for other works by this author on: Oxford Academic Google Scholar Norio Shiomi Norio Shiomi Department of Food Science, Faculty of Dairy Science, Rakuno Gakuen University, Ebetsu, Hokkaido 069, Japan To whom correspondence should be addressed. Search for other works by this author on: Oxford Academic Google Scholar Agricultural and Biological Chemistry, Volume 52, Issue 10, 1 October 1988, Pages 2569–2576, https://doi.org/10.1080/00021369.1988.10869080 Published: 01 October 1988 Article history Received: 12 May 1988 Published: 01 October 1988
Membrane Processes for Plasma Separation and Plasma Fractionation: Guiding Principles for Clinical Use (2001)

The use of membranes in blood processing range from the use of large-pore structures for filtration of blood for the removal of large particles (>20 microm transfusion filters) to membranes for the dialysis of blood for the removal of low molecular weight solutes in the treatment of renal failure. Within the past 20 years, membranes have been applied in the separation of plasma from whole blood. Compared to centrifugal plasma separation membrane, plasma separation is preferred when used with online plasma fractionation since the plasma is free of cells. In addition, membranes have also been applied in the online treatment of plasma for the selective removal of macromolecules in selected disease states obviating the need for plasma replacement products. The membranes used for plasma separation and fractionation may be distinguished from conventional dialysis membranes and high-flux membranes used in hemofiltration by their very high or select passage of plasma proteins. Membrane techniques are simple and safe to apply and can be competitive to other plasma separation and treatment technologies.
AgNPs-based Label-Free Colloidal SERS Nanosensor for the Rapid and Sensitive Detection of Stress-Proteins Expressed in Response to Environmental-Toxins (2013)

Among several physical, chemical and immunoassay-based methods for the detection of biomolecules, the Enzyme-Linked Immuno-Sorbent Assay (ELISA) is the standard technique that is routinely used for quantification of known proteins. However, it is a label-based, end-point sensor technique that is time-consuming, labor-intensive and fairly costly. This sandwich assay typically involves a series of peptide binding and washing steps. Here, we report a Surface-Enhanced Raman Spectroscopy (SERS) immuno-nanosensor technique that allows rapid and label-free extracellular detection of proteins compared to ELISA, and can potentially be used for intracellular detection. Our study shows that the silver nanoparticles (AgNPs) based SERS sensor can detect the stress-proteins, HSP70 and RAD54 expressed by yeast in response to environmental-toxins, in a dose dependent manner. As compared to the multi-step sandwich ELISA technique, the detection of stress-proteins using the SERS sensor is a simple two-step process. The simplicity of the SERS nanosensor design allowed the rapid detection of proteins within two hours in a fairly cost-effective and user-friendly approach. The SERS sensor we reported has an edge over ELISA as it directly quantifies the proteins without using any label (label-free) and also gives qualitative information about the antigen-antibody interaction. The SERS sensor showed good correlation and comparable sensitivity with ELISA. However, SERS was found to be less reproducible. Compared to previous reports on SERS-based protein detection techniques, our colloidal SERS sensor is easy to fabricate, offers improved biocompatibility, and allows rapid detection of the proteins in a cellular environment at picogram-levels. As a result, the SERS sensor demonstrates great potential for biomedical and environmental sensor technology (BEST) allowing label-free, rapid and sensitive detection, and could possibly replace ELISA.
A Highly Efficient and Linear Class-AB/F Power Amplifier for Multimode Operation (2008)

The class-AB/F power amplifier (PA), a multimode PA, which can operate at both class-AB and class-F modes, is analyzed and compared with the conventional class-F and class-AB PAs. The open-circuited third harmonic control circuit enhances the efficiency of the PA without deteriorating the linearity of class-AB mode of the PA. The voltage and current waveforms are simulated to evaluate the appropriate operation for the modes. To demonstrate the multimode PA, the PA is implemented using an InGaP/GaAs HBT process and it is tested with reverse-link IS-95A code division multiple access (CDMA) and PCS1900 global system for mobile communications signals in the personal communications service band. The class-AB operation for a CDMA signal delivers a power-added efficiency (PAE) of 38.9% and an adjacent channel power ratio of 49.5 and 56.5 dBc at the offset of 1.25 and 2.25 MHz, respectively, at the output power of 28 dBm. The maximum PAE of 64.7% under the class-F operation is measured at 32.5-dBm output power for a GSM signal. The class-AB/F PA is a good candidate for the multimode PA of next-generation wireless communication systems.
Effects of amniotic membrane suspension in the rat alkali burn model. (2011)

The suspension form of the amniotic membrane promoted epithelial healing and reduced corneal opacity and NV in alkali burn. It also suppressed F4/80 expression in the corneal stroma, indicating that the AM suspension maintains its beneficial biochemical effect on inflammatory corneal wound healing in vivo.