gebhart/patrepeval huggingface dataset:
A Measure for Dialog Complexity and its Application in Streamlining Service Operations (2017)

Dialog is a natural modality for interaction between customers and businesses in the service industry. As customers call up the service provider, their interactions may be routine or extraordinary. We believe that these interactions, when seen as dialogs, can be analyzed to obtain a better understanding of customer needs and how to efficiently address them. We introduce the idea of a dialog complexity measure to characterize multi-party interactions, propose a general data-driven method to calculate it, use it to discover insights in public and enterprise dialog datasets, and demonstrate its beneficial usage in facilitating better handling of customer requests and evaluating service agents.
Imaging acousto-optic tunable filter with 035-micrometer spatial resolution (1996)

Image blur in acousto-optic tunable filters (AOTF's) has been a persistent problem. Here we describe the connection between transducer structure and image blur and experimentally measure it by using a 5-cm 12°-cut TeO(2) crystal of our design. With these quantitative results, we develop an image-processing method that minimizes AOTF-related image degradation. The combination of long crystal design and image processing results in substantially improved image contrast and spatial resolution relative to conventional AOTF imaging devices. We present high-magnification images of fluorescent actin fibers in cells in which we obtain a resolution of approximately 0.35 μm, representing the first successful use of an AOTF for ultra-high-resolution microscopy. Further improvements are also predicted.
Cost-effective characterization of dissipative loss of printed circuit board traces (2016)

Determining how to accurately estimate and control the loss of a channel is critical to the success of the design of a high-speed I/O link. As Printed circuit boards (PCB) continue to be a key ingredient of a computer system design, the cost-effective characterization of the PCB materials and the measurement of the loss PCB traces in a given stackup have become a necessary step in the computer design cycle. In this paper, the measurement methods for the PCB trace loss are discussed and compared. A cost-effective method, namely Delta-L, is introduced and analyzed in detail. The method has been widely applied in the loss characterization of PCB traces in the server computer system designs. The simulation and measurement results are presented to show the effectiveness and accuracy of the method.
ARD 1, a 64-kDa guanine nucleotide-binding protein with a carboxyl-terminal ADP-ribosylation factor domain. (1993)

Clones referred to as ARD 1 were isolated from human and rat cDNA libraries. ARD 1 genes encode a putative 64-kDa protein that contains an 18-kDa ADP-ribosylation factor (ARF) domain at the carboxyl terminus and is much larger than the other monomeric approximately 20-kDa guanine nucleotide-binding ARF proteins thus far identified. ARD 1 mRNAs of 3.7 and 4.1 kilobases were detected in all rat tissues as well as in mouse and rabbit brain, human fibroblasts, and human neuroblastoma cells but not in HL-60 cells. Based on sequence identities, ARD 1 is highly conserved between rat and human. The ARF domain of ARD 1 contains the consensus sequences believed to be involved in guanine nucleotide binding, which are conserved in the ARFs and other GTP-binding proteins. Recombinant ARD 1 or the ARF domain of ARD 1, which lacks the 15 amino acids corresponding to the amino-terminal regions of ARFs stimulated, in a GTP-dependent manner, cholera toxin ADP-ribosyltransferase activity in the presence of 0.3% Tween 20. It had no effect in the presence of SDS, dimyristoylphosphatidylcholine/cholate, or cardiolipin. These observations are consistent with the conclusion that the amino-terminal region of ARF proteins is not required for activation of cholera toxin. In addition, the characteristic features of ARF proteins may be found as domains of larger mammalian proteins.
A Novel Miniature Interlacing Vibrating Electric Field Sensor (2006)

The miniature electric field sensor (EFS) presented in this paper consists of substrate, driving structure, shielding electrode and sensing electrode. Compared with its counterparts based on perpendicular and parallel vibrating structures driven by electric force with large driving voltage, the EFS, which employs a novel interlacing vibrating and sensing mode, increases the sensing signal remarkably. By employing piezoelectric ceramics driving structure, we realized a large displacement of the shielding electrode with a lower driving voltage of 2V, therefore reducing the crosstalk significantly. The experimental results show that the novel EFS has good dynamic stability, high S/N ratio and fine resolution of 100 V/m.
Eleven Amino Acid Glucagon-like Peptide-1 Receptor Agonists with Antidiabetic Activity (2009)

Glucagon-like peptide 1 (GLP-1) is a 30 or 31 amino acid peptide hormone that contributes to the physiological regulation of glucose homeostasis and food intake. Herein, we report the discovery of a novel class of 11 amino acid GLP-1 receptor agonists. These peptides consist of a structurally optimized 9-mer, which is closely related to the N-terminal 9 amino acids of GLP-1, linked to a substituted C-terminal biphenylalanine (BIP) dipeptide. SAR studies resulted in 11-mer GLP-1R agonists with similar in vitro potency to the native 30-mer. Peptides 21 and 22 acutely reduced plasma glucose excursions and increased plasma insulin concentrations in a mouse model of diabetes. These peptides also showed sustained exposures over several hours in mouse and dog models. The described 11-mer GLP-1 receptor agonists represent a new tool in further understanding GLP-1 receptor pharmacology that may lead to novel antidiabetic agents.
Classifying launch/impact events of mortar and artillery rounds utilizing DWT-derived features and feedforward neural networks (2006)

Feature extraction methods based on the discrete wavelet transform (DWT) and multiresolution analysis are used to develop a robust classification algorithm that reliably discriminates between launch and impact artillery and/or mortar events via acoustic signals produced during detonation. Distinct characteristics are found within the acoustic signatures since impact events emphasize concussive and shrapnel effects, while launch events are similar to explosions, designed to expel and propel an artillery round from a gun. The ensuing signatures are readily characterized by variations in the corresponding peak pressure and rise time of the waveform, differences in the ratio of positive pressure amplitude to the negative amplitude, variations in the prominent frequencies associated with the blast events and variations in the overall duration of the resulting waveform. Unique attributes can also be identified that depend upon the properties of the gun tube, projectile speed at the muzzle, and the explosive/concussive properties associated with the events. In this work, the discrete wavelet transform is used to extract the time-frequency components characteristic of the aforementioned acoustic signatures at ranges exceeding 2km. The resulting decomposition of the acoustic transient signals is used to produce a separable feature space. Highly reliable classification is achieved with a feedforward neural network classifier trained on a sample space derived from the distribution of wavelet coefficients and higher frequency details found within different levels of the multiresolution decomposition. The neural network developed herein provides a capability to classify events (as either launch (LA) or impact (IM)) with an accuracy that exceeds 88%.
Author Response: Deep Brain Stimulation in Early-Stage Parkinson Disease: Five-Year Outcomes (2021)

We appreciate the interest in our research1 of subthalamic nucleus (STN) deep brain stimulation (DBS) in early-stage Parkinson disease (PD). As noted in our publication, “Participants returned to the Vanderbilt Clinical Research Center (CRC) for annual outpatient study visits at years 3, 4, and 5, undergoing evaluations similar to the day 1 assessments conducted during the initial 2-year trial.” We would like to clarify that off -therapy tremor scores were not collected in follow-up years 3, 4, or 5 because of a lack of funding. We agree that reduced dyskinesia was expected because of the long-standing history of STN DBS being a potent therapy to reduce medications, and therefore dyskinesia, in advanced-stage Parkinson disease. We are, however, unaware of any other study that has shown that STN DBS reduces the risk of polypharmacy. This is an important new finding from the pilot trial of DBS in early-stage PD,1,2 given the well-established adverse effects associated with polypharmacy in older adults.3,4 The results of this study are encouraging, and we look forward to leading the FDA-approved [IDEG050016] phase III, multicenter, clinical trial of bilateral subthalamic nucleus deep brain stimulation implanted during very early-stage Parkinson disease.
Application of MassSQUIRM for Quantitative Measurements of Lysine Demethylase Activity (2012)

Recently, epigenetic regulators have been discovered as key players in many different diseases (1-3). As a result, these enzymes are prime targets for small molecule studies and drug development( 4). Many epigenetic regulators have only recently been discovered and are still in the process of being classified. Among these enzymes are lysine demethylases which remove methyl groups from lysines on histones and other proteins. Due to the novel nature of this class of enzymes, few assays have been developed to study their activity. This has been a road block to both the classification and high throughput study of histone demethylases. Currently, very few demethylase assays exist. Those that do exist tend to be qualitative in nature and cannot simultaneously discern between the different lysine methylation states (un-, mono-, di- and tri-). Mass spectrometry is commonly used to determine demethylase activity but current mass spectrometric assays do not address whether differentially methylated peptides ionize differently. Differential ionization of methylated peptides makes comparing methylation states difficult and certainly not quantitative (Figure 1A). Thus available assays are not optimized for the comprehensive analysis of demethylase activity. Here we describe a method called MassSQUIRM (mass spectrometric quantitation using isotopic reductive methylation) that is based on reductive methylation of amine groups with deuterated formaldehyde to force all lysines to be di-methylated, thus making them essentially the same chemical species and therefore ionize the same (Figure 1B). The only chemical difference following the reductive methylation is hydrogen and deuterium, which does not affect MALDI ionization efficiencies. The MassSQUIRM assay is specific for demethylase reaction products with un-, mono- or di-methylated lysines. The assay is also applicable to lysine methyltransferases giving the same reaction products. Here, we use a combination of reductive methylation chemistry and MALDI mass spectrometry to measure the activity of LSD1, a lysine demethylase capable of removing di- and mono-methyl groups, on a synthetic peptide substrate (5). This assay is simple and easily amenable to any lab with access to a MALDI mass spectrometer in lab or through a proteomics facility. The assay has ~8-fold dynamic range and is readily scalable to plate format (5).
Brillouin scattering in externally modulated lightwave AM-VSB CATV transmission systems (1992)

The authors measure the stimulated Brillouin scattering threshold for a CW (continuous wave) laser externally modulated with an AM-VSB (amplitude modulation vestigial sideband) CATV (cable television) signal. The threshold is essentially unchanged from the unmodulated case. For injected powers above the threshold power, substantial degradation of the carrier to noise ratio is seen.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Element-Coded Affinity Tags for Peptides and Proteins (2004)

Isotope-coded affinity tags (ICAT) represent an important new tool for the analysis of complex mixtures of proteins in living systems [Aebersold, R., and Mann, M. (2003) Nature, 422, 198-207]. We envisage an alternative protein-labeling technique based on tagging with different element-coded metal chelates, which affords affinity chromatography, quantification, and identification of a tagged peptide from a complex mixture. As proof of concept, a synthetic peptide was modified at a cysteine side chain with either a carboxymethyl group or acetamidobenzyl-1,4,7,10-tetraazacyclododecane-N,N',N' ',N' "-tetraacetic acid (AcBD) chelates of terbium or yttrium. A mixture of the three modified peptides in a mole ratio of 100:1.0:0.83 carboxymethyl:AcBD-Tb:AcBD-Y was trypsinized, purified on a new affinity column that binds rare-earth DOTA chelates, and analyzed by LC-MS/MS. Chelate-tagged tryptic peptides eluted cleanly from the affinity column; the tagged peptides chromatographically coeluted during LC-MS analysis, were present in the expected ratio as indicated by MS ion intensity, and were sequence-identified by tandem mass spectrometry. DOTA-rare earth chelates have exceptional properties for use as affinity tags. They are highly polar and water-soluble. Many of the rare earth elements are naturally monoisotopic, providing a variety of simple choices for preparing mass tags. Further, the rare earths are heavy elements, whose mass defects give the masses of tagged peptides exact values not normally shared by molecules that contain only light elements.
Validating a wheelchair in-seat activity tracker (2021)

Wheelchair users often experience prolonged periods of stationary sitting. Such periods are accompanied with increased loading of the ischial tuberosities. This can lead to the development of pressure ulcers which can cause complications such as sepsis. Periodic pressure offloading is recommended to reduce the onset of pressure ulcers. Experts recommend the periodic execution of different movements to provide the needed pressure offloading. Wheelchair users, however, might not remember to perform these recommended movements in terms of both quality and quantity. A system that can detect such movements could provide valuable feedback to both wheelchair users as well as clinicians. The objective of this study was to present and validate the WiSAT - a system for characterizing in-seat activity for wheelchair users. WiSAT is designed to detect two kinds of movements - weight shifts and in-seat movements. Weight shifts are movements that offload pressure on ischial tuberosities by 30% as compared to upright sitting and are maintained for 15 seconds. In-seat movements are shorter transient movements that involve either a change in the center of pressure on the sitting buttocks or a transient reduction in total load by 30%. This study validates the use of WiSAT in manual wheelchairs. WiSAT has a sensor mat which was inserted beneath a wheelchair cushion. Readings from these sensors were used by WiSAT algorithms to predict weight shifts and in-seat movements. These weight shifts and in-seat movements were validated against a high-resolution interface pressure mat in a dataset that resembles real-world usage. The proposed system achieved weight shift precision and recall scores of 81% and 80%, respectively, while in-seat movement scores were predicted with a mean absolute error of 22%. Results showed that WiSAT provides sufficient accuracy in characterizing in-seat activity in terms of weight shifts and in-seat movement.
A frequency selection scheme for increased imaging speed in ECT (2013)

Electrical capacitance tomography (ECT) is a method to determine the material distribution within the interior of a closed object by measuring the capacitance values across externally mounted electrodes. Traditionally, an ac excitation pulse is applied to a pair of electrodes that form a capacitor during each measurement step, in order to determine the capacitance from the output current measured. This paper investigates how the speed of inter-electrode capacitance measurement can be improved by applying and receiving multiple excitation signals simultaneously on multiple electrodes. An excitation frequency selection scheme is derived to discuss the effect of bandwidth on the interference between the simultaneously sampled measurement channels. The new scheme opens up new possibilities for ECT as an effective tool for online, real-time monitoring of a wide range of dynamical processes.
Efficient and stable approach to elasticity and collisions for hair animation (2015)

We present a hybrid direct and projective iterative algorithm for simulating hair. The algorithm is fast, stable, visually accurate, uses a comprehensive elasticity model with a well defined continuum limit. The low resolution simulation is a good predictor of higher resolution results which we attribute to our novel treatment of bending and twisting forces. Timesteps may be as large a single step for each frame of 24 Hz animation. The treatment of collisions is implicit and does not introduce any artificial strain or forces.
Photopolarimeter based on planar grating diffraction (1993)

A division-of-amplitude photopolarimeter (DOAP) is described that employs a diffraction grating in the conventional spectrometer orientation with the grating grooves normal to the plane of incidence. Four coplanar diffracted orders are used for polarimetric analysis to determine all four Stokes parameters of incident light simultaneously and virtually instantaneously (with the speed being determined solely by the photodetectors and their associated electronics); a fifth order is used for alignment by autocollimation or by use of a position-sensing quadrant detector. To sensitize the instrument for the +45° and −45° azimuths of incident linearly polarized light and for the handedness of incident circular polarization (i.e., for the third and fourth Stokes parameters), we insert two linear polarizers in two diffracted orders with their transmission axes inclined at appropriate angles with respect to the plane of incidence. The calibration and testing of an instrument of this type that uses an Al-coated 600-groove/mm holographic grating at 632.8-nm wavelength are reported as an example.
A hybrid silicon-phosphorene nanolaser (2017)

We show evidence of lasing from a hybrid nanostructure composed of a silicon optical resonator and a two-dimensional phosphorene film. The ~1555 nm emission wavelength opens possibilities for optically-active devices for integrated silicon photonics.
Evaluation of deep convolutional neural networks in classifying human embryo images based on their morphological quality (2021)

A critical factor that influences the success of an in-vitro fertilization (IVF) treatment cycle is the quality of the transferred embryo. Embryo morphology assessments, conventionally performed through manual microscopic analysis suffer from disparities in practice, selection criteria, and subjectivity due to the experience of the embryologist. Convolutional neural networks (CNNs) are powerful, promising algorithms with significant potential for accurate classifications across many object categories. Network architectures and hyper-parameters affect the efficiency of CNNs for any given task. Here, we evaluate multi-layered CNNs developed from scratch and popular deep-learning architectures such as Inception v3, ResNET-50, Inception-ResNET-v2, NASNetLarge, ResNeXt-101, ResNeXt-50, and Xception in differentiating between embryos based on their morphological quality at 113 h post insemination (hpi). Xception performed the best in differentiating between the embryos based on their morphological quality.
A new crosslinkable system based on thermal Huisgen reaction to enhance the stability of electro-optic polymers (2009)

The thermal Huisgen cycloaddition reaction is a new and efficient process to crosslink electro-optic polymers, since it leads to an effective increase of the thermal alignment stability of the NLO chromophores.
Direct Imaging of Cl‐ and Cu‐Induced Short‐Circuit Efficiency Changes in CdTe Solar Cells (2014)

To achieve high‐efficiency polycrystalline CdTe‐based thin‐film solar cells, the CdTe absorbers must go through a post‐deposition CdCl 2 heat treatment followed by a Cu diffusion step. To better understand the roles of each treatment with regard to improving grains, grain boundaries, and interfaces, CdTe solar cells with and without Cu diffusion and CdCl 2 heat treatments are investigated using cross‐sectional electron beam induced current, electron backscatter diffraction, and scanning transmission electron microscope techniques. The evolution of the cross‐sectional carrier collection profile due to these treatments that cause an increase in short‐circuit current and higher open‐circuit voltage are identified. Additionally, an increased carrier collection in grain boundaries after either/both of these treatments is revealed. The increased current at the grain boundaries is shown to be due to the presence of a space charge region with an intrinsic carrier collection profile width of ≈350 nm. Scanning transmission electron microscope electron‐energy loss spectroscopy shows a decreased Te and increased Cl concentration in grain boundaries after treatment, which causes the inversion. Each treatment improves the overall carrier collection efficiency of the cell separately, and, therefore, the benefits realized by each treatment are shown to be independent of each other.
Subpopulations of GFP-Marked Mouse Pancreatic β-Cells Differ in Size, Granularity, and Insulin Secretion (2012)

There is growing information about the heterogeneity of pancreatic β-cells and how it relates to insulin secretion. This study used the approach of flow cytometry to sort and analyze β-cells from transgenic mice expressing green fluorescent protein (GFP) under the control of the mouse insulin I gene promoter. Three populations of β-cells with differing GFP brightness could be identified, which were classified as GFP-low, GFP-medium, and GFP-bright. The GFP-medium population comprised about 70% of the total. The GFP-low population had less insulin secretion as determined by the reverse hemolytic plaque assay and reduced insulin gene expression. Additionally, all three subpopulations of β-cells were found in mice of varying ages (embryonic d 15.5 and postnatal wk 1-9). The three populations from the youngest had larger cells (forward scatter) and less granularity (side scatter) than those from the adults. This approach opens up new ways to advance knowledge about β-cell heterogeneity.