714 URINE KIDNEY INJURY MOLECULE-1 A NON-INVASIVE SCREENING TOOL FOR PATIENTS WITH RENAL CELL CARCINOMA (2012)

You have accessJournal of UrologyKidney Cancer: Evaluation and Staging II1 Apr 2012714 URINE KIDNEY INJURY MOLECULE-1 A NON-INVASIVE SCREENING TOOL FOR PATIENTS WITH RENAL CELL CARCINOMA Joseph Mashni, Venkata Sabasetti, Ping Zhang, Kenneth Kernen, Brian Seifman, Mitual Amin, Joseph Bonventre, and Jason Hafron Joseph MashniJoseph Mashni Royal Oak, MI , Venkata SabasettiVenkata Sabasetti Boston, MA , Ping ZhangPing Zhang Royal Oak, MI , Kenneth KernenKenneth Kernen Royal Oak, MI , Brian SeifmanBrian Seifman Royal Oak, MI , Mitual AminMitual Amin Royal Oak, MI , Joseph BonventreJoseph Bonventre Boston, MA , and Jason HafronJason Hafron Royal Oak, MI View All Author Informationhttps://doi.org/10.1016/j.juro.2012.02.798AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Kidney injury molecule-1 (KIM-1) is a type I transmembranous protein and specific injury marker of renal proximal tubules. Our previous study demonstrates that KIM-1 is prominently up regulated in proximal tubule-derived renal cell carcinoma (RCC), including clear cell and papillary RCC, but not in chromophobe RCC (a distal tubule tumor). Additionally, pre-operative KIM-1 levels in urine samples were significantly elevated in patients with RCC. This study was designed to examine urine KIM-1 level before and 1 month after surgical removal of renal tumors. METHODS 19 patients with localized radiographic renal lesions were prospectively enrolled into the study. Pre-operative and post-operative urine KIM-1 levels were measured using a human KIM-1 ELISA. The urine KIM-1 levels were normalized by urine creatinine levels as a urine KIM-1/creatinine ratio (UKCR). Renal tumors were also stained for KIM-1, using an immuno-histochemical AKG7 anti-KIM-1 antibody, which divided patients into a KIM-1 positive or negative group. RESULTS The KIM-1 negative group included 7 cases (2 chromophobe RCC, 4 benign cystic lesions and one hematoma), and the KIM-1 positive group was composed of 12 cases (6 C-RCC, 5 P-RCC and 1 XP11.2 translocation RCC). In both groups, serum creatinine (sCR) levels were significantly elevated after nephrectomy. In the KIM-1 negative group, sCr levels increased from pre-operative level of 1.011 ± 0.053 mg/dl to post-operative level of 1.366 ± 0.150 mg/dL, and in the KIM-1 positive group the pre-sCr level of 1.012 ± 0.094 mg/dl significantly increased to 1.447 ± 0.129 mg/dl. In the KIM-1 negative group, UKCR remained at a similar level before (0.367 ± 0.095 ng/mg Cr) and after nephrectomy (0.323 ± 0.014 ng/mg Cr). However, in the KIM-1 positive group, the elevated UKCR at 1.200 ± 0.310 ng/mg Cr, was significantly reduced to 0.362 ± 0.099 ng/mg Cr, after nephrectomy. CONCLUSIONS Our small but prospective study showed significant reduction in UKCR after nephrectomy, suggesting that urine KIM-1 appears to be a non-invasive pre-operative measure to evaluate the malignant potential of renal masses and may also represent a surrogate biomarker for KIM-1 positive RCC. © 2012 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 187 Issue 4S April 2012 Page: e293 Advertisement Copyright & Permissions© 2012 by American Urological Association Education and Research, Inc.Metrics Author Information Joseph Mashni Royal Oak, MI More articles by this author Venkata Sabasetti Boston, MA More articles by this author Ping Zhang Royal Oak, MI More articles by this author Kenneth Kernen Royal Oak, MI More articles by this author Brian Seifman Royal Oak, MI More articles by this author Mitual Amin Royal Oak, MI More articles by this author Joseph Bonventre Boston, MA More articles by this author Jason Hafron Royal Oak, MI More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...