gebhart/patrepeval huggingface dataset:
Hybrid integration of scalable mechanical and magnetophoretic focusing for magnetic flow cytometry (2018)

Time-of-flight (TOF) magnetic sensing of rolling immunomagnetically-labeled cells offers great potential for single cell function analysis at the bedside in even optically opaque media, such as whole blood. However, due to the spatial resolution of the sensor and the low flow rate regime required to observe the behavior of rolling cells, the concentration range of such a workflow is limited. Potential clinical applications, such as testing of leukocyte function, require a cytometer which can cover a cell concentration range of several orders of magnitude. This is a challenging task for an integrated dilution-free workflow, as for high cell concentrations coincidences need to be avoided, while for low cell concentrations sufficient statistics should be provided in a reasonable time-to-result. Here, we extend the spatial bandwidth of a magnetoresistive sensor with an adaptive and integratable workflow concept combining mechanical and magnetophoretic guiding of magnetically labeled targets for in-situ enrichment over a dynamic concentration range of 3 orders of magnitude. We achieve hybrid integration of the enrichment strategy in a cartridge mold and a giant-magnetoresistance (GMR) sensor in a functionalized Quad Flat No-Lead (QFN) package, which allows for miniaturization of the Si footprint for potential low-cost bedside testing. The enrichment results demonstrate that TOF magnetic flow cytometry with adaptive particle focusing can match the clinical requirements for a point-of-care (POC) cytometer and can potentially be of interest for other sheath-less methodologies requiring workflow integration.
Integrated hollow and solid-core waveguides for sensor platforms (2005)

We report the fabrication of hollow and solid-core waveguide structures on planar substrates. These structures are suitable for low-loss anti-resonant reflecting (ARROW) and photonic crystal waveguides, and integration with microfluidic or gas-sensing platforms.
Chemical modification of everninomicins. (1982)

Novel antibiotic everninomicin D is chemically transformed into new biologically active derivatives. Reactions of a nitro group attached to a tertiary carbon center have been investigated. Synthesis and reactions of hydroxylaminoeverninomicin D, aminoeverninomicin D and their derivatives have been discussed.
Measurement of urinary cauxin in geriatric cats with variable plasma creatinine concentrations and proteinuria and evaluation of urine cauxin-to-creatinine concentration ratio as a predictor of developing azotemia (2010)

The UC/C did not vary with severity of azotemia but appeared contributory to the feline urinary proteome. High UC/C values were predictive of the geriatric cats in our study developing azotemia. However, it seems unlikely that UC/C will provide additional information about the measurement of urine protein-to-creatinine concentration ratio as a biomarker for the development of azotemia in cats.
Association between polymorphism in p21(Waf1/Cip1) cyclin-dependent kinase inhibitor gene and human oral cancer. (2000)

The cyclin-dependent kinase inhibitor gene p21(Waf1/Cip1) plays a central role in inducing cellular growth arrest, terminal differentiation, and apoptosis. Alterations in this gene may adversely affect regulation of these processes and increase susceptibility for cancer. We have recently reported a novel polymorphism in the p21(Waf1/Cip1) gene in the Indian population and its association with esophageal cancer. An A-->G transition at codon 149 resulted in amino acid substitution from aspartate to glycine in the proliferating cell nuclear antigen binding COOH-terminal domain of p21(Waf1/Cip1) that may affect PCNA-p21(Waf1/Cip1) interactions, thereby affecting regulation of cellular proliferation, and may increase susceptibility for development of cancer. In a parallel study in our laboratory, we searched for putative p21(Waf1/Cip1) mutations in oral premalignant and malignant lesions. No somatic mutation was detected in exon 2 of p21(Waf1/Cip1). Interestingly, a codon 149 polymorphism variant (A-->G) was identified in 11 of 30 (37%) premalignant lesions (7 of 19 hyperplastic lesions and 4 of 11 dysplastic lesions) and 11 of 30 (37%) squamous cell carcinomas (SCCs). This codon 149 variant was also identified in paired lymphocytes of all of the patients with premalignant lesions and SCCs harboring the variant allele, suggesting the occurrence of a polymorphism. Lymphocyte DNA isolated from 50 unrelated age- and gender-matched healthy subjects was screened for this polymorphism. Seven of 50 (14%) normal controls harbored the A-->G codon 149 variant allele. Immunohistochemical analysis of p21(Waf1/Cip1) protein expression showed immunoreactivity in 19 of these 30 (63%) oral premalignant lesions and 16 of 30 (53%) SCCs. The most intriguing features of the study were: (a) the significant increase in frequency of this polymorphism not only in patients with oral SCCs (P = 0.038), but also in patients with premalignant lesions (P = 0.038), compared with normal controls; and (b) the significantly higher frequency of p21(Waf1/Cip1) variants (codon 149) in oral premalignant lesions (10 of 11 cases) and SCCs (11 of 11 cases) with wild-type p53 (P = 0.045) than in lesions with p53 mutations, suggesting that this polymorphism affects the p53 pathway and may play a vital role in oral tumorigenesis. Furthermore, overexpression of p21 protein in oral lesions harboring missense mutations in the p53 gene suggest a p53-independent role for p21 in the pathogenesis of oral cancer.
Ant routing algorithm for mobile ad-hoc networks (ARAMA) (2003)

Routing algorithms in mobile ad-hoc networks (MANETs) should match the special characteristics of MANETs. It should be A self-configured, self-built and distributed routing algorithm. In addition, it should be able to optimize more than one quality of service parameter to achieve good network performance. The remaining energy in each node across the network should be fairly distributed. We are propose the ant routing algorithm for Mobile Ad-hoc networks (ARAMA), which is a biological based routing algorithm. This algorithm is inspired from the ant colonies' life. Forward packets are used to collect information about the network and backward packets are used to update the routing information in the nodes. The paper provides a description of the algorithm. Simulation results show the ability of the algorithm to fairly distribute the remaining energy across the network nodes and solve the MANETs routing problem under different dynamic conditions.
Evaluating the utility of intraprocedural 3D TRUS image information in guiding registration for displacement compensation during prostate biopsy (2014)

While the majority of the registrations using 2D TRUS images provided a clinically desired level of accuracy, intraprocedural 3D imaging helped improve the overall registration accuracy and robustness, especially in the base and apex regions of the prostate. These results are helpful for devising image-based registration methods for displacement compensation when designing 3D TRUS-guided biopsy systems.
Object Retrieval Using Visual Query Context (2011)

Object retrieval aims at retrieving images containing objects similar to the query object captured in the region of interest (ROI) of the query image. Boosted by the invention and wide popularity of SIFT image features and bag-of-visual-words image representation, object retrieval has progressed significantly in the past years and has already found deployment in real-life applications and products. While existing object retrieval methods perform well in many cases, they may fail to return satisfactory results if the ROI specified by the user is inaccurate or if the object captured there is too small to be represented using discriminative features and consequently to be matched with similar objects in the image collection. In order to improve the object retrieval performance also in these difficult cases, we propose in this paper an object retrieval method that exploits the information about the visual context of the query object and employ it to compensate for possible uncertainty in feature-based query object representation. Contextual information is drawn from the visual elements surrounding the query object in the query image. We consider the ROI as an uncertain observation of the latent search intent and the saliency map detected for the query image as a prior. Then a language modeling approach is employed to devise a contextual object retrieval (COR) model. There, the relevance score is determined based on the search intent scores that are inferred from the uncertain ROI and the saliency prior. The usefulness of the contextual information for object retrieval and the effectiveness of the proposed COR model are demonstrated and evaluated on three representative image datasets.
Wireless power transfer through strongly Coupled Electric Resonance (2013)

Wireless power transfer via novel Strongly Coupled Electric Resonance (SCER) method is presented and studied in this paper. Instead of using loops as the magnetic resonators in Strongly Coupled Magnetic Resonance (SCMR), small dipoles are introduced to be considered as electric resonators between source and load. High wireless powering efficiency (more than 95%) and compact size (50% smaller than traditional SCMR) are achieved. Additionally, by changing the value of the lumped inductance, it is easier to shift the frequency and maintain high transmission efficiency.
Dielectric properties of supramolecular ionic structures obtained from multifunctional carboxylic acids and amines (2014)

Dielectric properties of ionic networks formed from the supramolecular assembly of amine and carboxy functionalised polymers were measured and analyzed.
Production Forecasting for Shale Gas Exploration Prospects Based on Statistical Analysis and Reservoir Simulation (2012)

Abstract Shale gas exploration and production is still in its infancy. Information is scarce and flow monitoring is usually absent, but many studies aiming to understand gas shale reservoir behavior and to optimize its exploitation are now being developed. However, the understanding of fluid flow and transportation mechanisms in shales, given their micropore particular matrix structure coupled to induced fractures, is still under debate. Several shale gas exploration projects are currently underway in diverse regions of Europe and Africa. The current lack of understanding of the main elements controlling well productivity, and the limitations of the available tools to correctly characterize the reservoir and forecast production from this type of well, make it difficult to predict the likely commercial value of a project. This value is controlled by drilling costs and the associated number of wells to develop a play, and the production returns that each of these wells may deliver. The purpose of the work presented in this paper was to generate, based on statistical analysis of major shale gas basins in the USA, a synthetic reservoir simulation model that can represent shale gas wells and be used for forecasting purposes in exploration areas with limited available information. Such a prediction is mainly based on the accurate identification of parameters that have the greatest impact on production, either reservoir parameters or operational/controlled parameters, so that the production profiles may be predicted and controlled parameters optimized.
Real‐time analog holography and pattern recognition (2001)

Abstract The combination of a high‐resolution charge‐coupled device (CCD) camera and a phase‐modulating liquid‐crystal array allows holographic fringes to be detected and redisplayed in real time, replacing traditional recording techniques. A modified Mach–Zehnder (Leith–Upatnieks) architecture supplies the small object‐to‐reference beam angle necessary to produce fringes large enough to be processed accurately by the recording system. The optical system, developed to produce holograms and display holographic reconstructions in real time, is then slightly changed to become an optical pattern‐recognition architecture. The real‐time holography system and the optical correlator modifications are described in detail, and examples of experimental results are presented. © 2001 John Wiley & Sons, Inc. Microwave Opt Technol Lett 30: 20–24, 2001.
Subharmonic Contrast Microbubble Signals for Noninvasive Pressure Estimation under Static and Dynamic Flow Conditions (2011)

Our group has proposed the concept of subharmonic aided pressure estimation (SHAPE) utilizing microbubble-based ultrasound contrast agent signals for the noninvasive estimation of hydrostatic blood pressures. An experimental system for in vitro SHAPE was constructed based on two single-element transducers assembled confocally at a 60 degree angle to each other. Changes in the first, second and subharmonic amplitudes of five different ultrasound contrast agents were measured in vitro at static hydrostatic pressures from 0-186 mmHg, acoustic pressures from 0.35-0.60 MPa peak-to-peak and frequencies of 2.5-6.6 MHz. The most sensitive agent and optimal parameters for SHAPE were determined using linear regression analysis and implemented on a Logiq 9 scanner (GE Healthcare, Milwaukee, WI). This implementation of SHAPE was then tested under dynamic-flow conditions and compared to pressure-catheter measurements. Over the pressure range studied, the first and second harmonic amplitudes reduced approximately 2 dB for all contrast agents. Over the same pressure range, the subharmonic amplitudes decreased by 9-14 dB and excellent linear regressions were achieved with the hydrostatic pressure variations (r = 0.98, p < 0.001). Optimal sensitivity was achieved at a transmit frequency of 2.5 MHz and acoustic pressure of 0.35 MPa using Sonazoid (GE Healthcare, Oslo, Norway). A Logiq 9 scanner was modified to implement SHAPE on a convex transducer with a frequency range from 1.5-4.5 MHz and acoustic pressures from 0-3.34 MPa. Results matched the pressure catheter (r2 = 0.87). In conclusion, subharmonic contrast signals are a good indicator of hydrostatic pressure. Out of the five ultrasound contrast agents tested, Sonazoid was the most sensitive for subharmonic pressure estimation. Real-time SHAPE has been implemented on a commercial scanner and offers the possibility of allowing pressures in the heart and elsewhere to be obtained noninvasively.
Implementation and evaluation of an accurate real-time voiceband signal classifier (2002)

This paper describes the implementation and resulting accuracy of an economical voiceband signal classifier developed for use in the public switched telephone network. Companded digital signals are extracted from a 1.544 Mbps T1 digital trunk and then classified into either silence or twelve other active categories, including speech, four classes of data modem, three classes of fax, random binary data, fax signalling, ringback signal, and dual tone multifrequency (DTMF) digits. The classifier first derives from the baseband signal in each channel the central second-order moment and the first ten lags of the autocorrelation sequence, all normalized with respect to average power. Avoiding demodulation and Fourier transform steps in the classification process permits linear and quadratic discriminant functions to be computed in real time for all 24 T1 channels. Classification accuracies are reported for statistically optimal linear discriminant functions over classification intervals ranging from 31.5 to 256.5 ms. Also given are the greater accuracies achievable using statistically optimal quadratic discriminant functions and an automatically trained decision tree known as an adaptive logic network (ALN).
Baseband carrier phase tracking technique for Gaussian minimum shift-keying (2000)

This paper presents a technique that provides the means to recover the carrier phase at baseband with high accuracy, while at the same time suffering negligible loss due to nonrandom data patterns. Simulation results for tracking performance are presented, as are the simulation results showing the effects of imperfect tracking performance on the bit error rate (BER) performance.
Chicken Egg White Cystatin (1989)

A lambda gt11 chicken oviduct cDNA library was screened with a mixed synthetic oligonucleotide corresponding to amino acid residues 81-90 of chicken egg white cystatin, a cysteine proteinase inhibitor. Two initial cDNA clones of 367 and 431 bases were isolated. Both clones contained coding sequences for cystatin from amino acid residue 82 to the carboxyl end plus 3'-untranslated region and a poly(A)+ tail. The two clones utilized different polyadenylation signals located 55 nucleotides apart. Further screening of the library yielded a full-length cystatin cDNA. Sequence analysis indicated that cystatin contains an NH2-terminal extension of 23 amino acids which is probably a signal sequence. The cystatin cDNA hybridized to an mRNA of approximately 0.95 kilobase and was present in varying amounts in all chicken tissues examined. The highest concentration was found in the lung. Gizzard, brain, and heart contained lesser amounts of cystatin mRNA but considerably higher than oviduct. Among a limited number of embryonic tissues examined, significantly higher levels of the mRNA were found in liver and heart tissues when compared with the corresponding adult tissues. These results suggested that the expression of the chicken cystatin gene is tissue-dependent and under developmental control.
High-Sensitivity Demodulation of Multiple-Data-Rate WDM-DPSK Signals using a Single Interferometer (2007)

We demonstrate simultaneous reception of multiple-rate wavelength-division-multiplexed optical- DPSK signals using a single interferometer. The demodulation approach provides rate-flexibility and scalability, enabling penalty-free performance and compliance with existing channel-rate and channel-spacing standards.
Syntheses, characterization, and reactivity of manganese and rhenium dioxime complexes. X-ray crystal structure of MnII(CDO)(CDOH)2(BPh(OCH3))2, an unusual pseudoclathrochelate complex, and ReIIICl(CDO)(CDOH)2BPh (1991)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSyntheses, characterization, and reactivity of manganese and rhenium dioxime complexes. X-ray crystal structure of MnII(CDO)(CDOH)2(BPh(OCH3))2, an unusual pseudoclathrochelate complex, and ReIIICl(CDO)(CDOH)2BPhS. Jurisson, L. Francesconi, K. E. Linder, E. Treher, M. F. Malley, J. Z. Gougoutas, and A. D. NunnCite this: Inorg. Chem. 1991, 30, 8, 1820–1827Publication Date (Print):April 1, 1991Publication History Published online1 May 2002Published inissue 1 April 1991https://pubs.acs.org/doi/10.1021/ic00008a026https://doi.org/10.1021/ic00008a026research-articleACS PublicationsRequest reuse permissionsArticle Views127Altmetric-Citations31LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
The substrate specificity of Uca pugilator collagenolytic serine protease 1 correlates with the bovine type I collagen cleavage sites. (1994)

Affinity-based purification and characterization of the collagenolytic serine protease 1 from Uca pugilator (fiddler crab) hepatopancreas shows that the enzyme cleaves the native bovine alpha 1(I) collagen chain carboxyl-terminal to Gln and Arg residues adjacent to the metallocollagenase site. Cleavage carboxyl-terminal to Leu residues is observed in the alpha 2(I) chain and at a secondary site in alpha 1(I). These sites correlate with the preferences observed toward p-nitroanilide substrates varying at the P1 position, for which the specificity (kcat/Km) is Arg > Leu, Phe, Lys > Gln > Ala. Furthermore, collagen cleavage after Gln was found exclusively between two Gln-Arg bonds. The P'1-P'3 specificity of collagenase, as determined by nucleophile acyl transfer, indicated a strong preference for Arg in the P'1 position. Crab collagenase cleaves peptide bonds adjacent to Leu and Gln at the P1 position more efficiently than trypsin, chymotrypsin, or elastase. Moreover, the efficiency of collagenase toward P1-Arg substrates is equivalent to that of trypsin. Crystals of crab collagenase have been grown complexed with the protein inhibitor ecotin. These crystals diffract to better than 2.8 A resolution and belong to the space group P3(2)21 with unit cell dimensions of a = b = 89.0 A, c = 291.7 A.
Cloning and identification of human Sca as a novel inhibitor of osteoclast formation and bone resorption. (1998)

Increased osteoclast activity is responsible for the enhanced bone destruction in postmenopausal osteoporosis, Paget's disease, bone metastasis, and hypercalcemia of malignancy. However, the number of known inhibitory factors that block osteoclast formation and bone resorption are limited. Therefore, we used an expression-cloning approach to identify novel factors produced by osteoclasts that inhibit osteoclast activity. A candidate clone was identified and isolated from a human osteoclast-like multinucleated cell (MNC) cDNA library, named osteoclast inhibitory peptide-1 (OIP-1), and the cDNA sequence was determined. This sequence matched that of the recently identified human stem cell antigen, was structurally similar to the mouse Ly-6 gene family, and the sequence predicted it was a glycosyl phosphatidyl inositol (GPI)-anchored protein that had a cleavable COOH-terminal peptide. Western blot analysis of conditioned media from 293 cells transfected with the OIP-1 cDNA clone confirmed that OIP-1 was released into the media as a membrane-bound GPI-linked protein. Interestingly, both recombinant OIP-1 expressed in Escherichia coli (which does not have GPI linker) and OIP-1 expressed by mammalian cells significantly reduced osteoclast-like MNC formation induced by 1,25-dihydroxyvitamin D3 or PTH-related protein in mouse and human bone marrow cultures, and inhibited 45Ca release from prelabeled bone in fetal rat organ cultures. In contrast, recombinant OIP-1 did not inhibit the growth of a variety of other cell types. These data indicate that OIP-1 is a novel, specific inhibitor of osteoclast formation and bone resorption.