gebhart/patrepeval huggingface dataset:
Invasive Pneumococcal Infections: A Comparison Between Adults and Children (1997)

A similar number of adults and children had invasive pneumococcal infection. There was male predominance, and different ethnic distribution between children and adults. The majority of adults (78%), had underlying diseases, but this was less frequent in children (24%). The presenting illness differed between adults and children. Complications of invasive pneumococcal infection occurred more frequently in adults than in children. The mortality rate in adults was 21.5%; in children, only 3.8%. The rate of penicillin-resistant pneumococci at our hospital was 23%, while cefotaxime resistance was 4.2%. Penicillin-resistant pneumococci were not isolated more frequently from children than from adults. Patients with penicillin-resistant pneumococci had longer duration of hospitalization and more nosocomially acquired infections. No difference in the mortality rate was found between patients with resistant or sensitive pneumococci. Ninety-five percent of strains were included in the current vaccine, but less than 2% of patients had been vaccinated. Isolates prevalent in Europe and the United States (19, 5, 1, 14, 6, 18, 12, 4, 9, 23, 7) were also most prevalent in Jerusalem. The distribution of serotypes differed between children and adults, and between patients from whom resistant organisms were isolated as opposed to sensitive organisms.
A surfactant‐based wound dressing can reduce bacterial biofilms in a porcine skin explant model (2016)

Bacterial biofilms have been found in many, if not all, chronic wounds. Their excessive extracellular matrix secretion and the metabolic changes that they undergo render them highly tolerant of many antibiotic and antimicrobial treatments. Physical removal and/or disruption are a common approach to treating wounds suspected of having bacterial biofilms. While many of these techniques use mechanical energy as the primary means of removal, we have begun to investigate if surfactants could facilitate the removal of bacterial biofilms, or if they might sensitise the biofilms to antimicrobial interventions. We tested a new surfactant-based wound gel on an ex vivo porcine skin explant model infected with a functionally tolerant 3-day biofilm. The wounds were dressed with a surfactant-based gel directly on the wound or with moistened gauze. The wounds were then wiped daily with moistened gauze, and the gel or gauze was re-applied. Each day, an explant from each group was harvested and tested for total viable bacteria counts and viable biofilm-protected bacteria counts. The results show that daily wiping with moistened gauze led to an initial decrease of bacteria, but by day 3, the biofilm had been fully re-established to the same level prior to the beginning of treatment. For the surfactant-based treatment, there was no detectable functional biofilm after the first treatment. The gauze control, which was also subjected to daily wiping, still contained functional biofilms, indicating that this result was not due to wiping alone. The total bacteria in the surfactant-treated explants steadily decreased through day 3, when there were no detectable bacteria, while the wiping-only control bacteria counts remained steady. The use of a moist gauze to wipe the visually apparent slime off of a wound appears to be insufficient to reduce biofilm over a 3-day period. Daily application of the surfactant gel dressing and wiping reduced the biofilm to undetectable levels within 3 days in a skin explant model. A 3-day regimen of dressing the wound model with a surfactant gel followed by gentle removal of the gel by wiping with a moistened gauze appears to be a simple and adequate approach to removing a bacterial biofilm infection in an ex vivo model. Additional clinical evidence is needed to determine if this promising approach can perform the same in clinically infected chronic wounds.
Non-invasive glucose monitoring using open electromagnetic waveguides (2012)

This paper presents a novel approach towards non-invasive glucose measurement. The introduced concept is based on an electromagnetic (EM) wave transmission along blood vessels. Theoretical investigation of a TEM wave propagation through a aqueous glucose solution is shown. The concept was evaluated by a simplified demonstrator which consists of a dielectric covered TEM-Waveguide. A 3D field simulation of the structure has been performed with a glucose dependent Debye relaxation model. The EM waveguide was fabricated and measured with various glucose concentrations. A phase difference of 12 deg for a 500mg/dl variation in glucose concentration was measured at 20GHz.
Experimental Models of Status Epilepticus (2019)

This chapter discusses each model of Status epilepticus (SE) from the perspective of how to induce SE, pathophysiology and neuropathology, postseizure care and behavior, the efficacy of standard anticonvulsant drugs, as well as the advantages and limitations of each model. Several experimental models of SE have been developed that approximate specific aspects of the clinical event and the subsequent alterations that occur in neuronal structure and function. The kainic acid model of SE is one of the most extensively studied seizure models. An important factor that must be taken into account when comparing the effectiveness of potential anticonvulsant treatments on SE is that the longer SE persists the more difficult it is to control. Kainic acid induces SE accompanied by extensive neuronal damage, synaptic reorganization, and spontaneous recurrent seizures in adult rats. An important advantage of being able to induce SE in a slice preparation is that it provides an easy way to test new anticonvulsant drugs.
Recruitment of Transcription Complexes to the β-Globin Gene Locus in Vivo and in Vitro (2004)

Erythroid-specific, high level expression of the beta-globin genes is regulated by the locus control region (LCR), composed of multiple DNase I-hypersensitive sites and located far upstream of the genes. Recent studies have shown that LCR core elements recruit RNA polymerase II (pol II). In the present study we demonstrate the following: 1) pol II and other basal transcription factors are recruited to LCR core hypersensitive elements; 2) pol II dissociates from and re-associates with the globin gene locus during replication; 3) pol II interacts with the LCR but not with the beta-globin gene prior to erythroid differentiation in embryonic stem cells; and 4) the erythroid transcription factor NF-E2 facilitates the transfer of pol II from immobilized LCR constructs to a beta-globin gene in vitro. The data are consistent with the hypothesis that the LCR serves as the primary attachment site for the recruitment of macromolecular complexes involved in chromatin structure alterations and transcription of the globin genes.
A new regulatory protein, KSRP, mediates exon inclusion through an intronic splicing enhancer. (1997)

We have purified and cloned a new splicing factor, KSRP. KSRP is a component of a multiprotein complex that binds specifically to an intronic splicing enhancer element downstream of the neuron-specific c-src N1 exon. This 75-kD protein induces the assembly of five other proteins, including the heterogeneous nuclear ribonucleoprotein F, onto the splicing enhancer. The sequence of the KSRP cDNA indicates that the protein contains four K homology RNA-binding domains and an unusual carboxy-terminal domain. KSRP is similar to two proteins, FUSE-binding protein and P-element somatic inhibitor. KSRP is expressed in both neural and non-neural cell lines, although it is present at higher levels in neural cells. Antibodies specific for KSRP inhibit the splicing of the N1 exon in vitro. Moreover, this inhibition of N1 splicing can be rescued by the addition of purified KSRP. KSRP is likely to regulate splicing from a number of intronic splicing enhancer sequences.
DLIME: A Deterministic Local Interpretable Model - Agnostic Explanations Approach for Computer-Aided Diagnosis Systems (2023)

<p>Local Interpretable Model-Agnostic Explanations (LIME) is a popular technique used to increase the interpretability and explainability of black box Machine Learning (ML) algorithms. LIME typically generates an explanation for a single prediction by any ML model by learning a simpler interpretable model (e.g. linear classifier) around the prediction through generating simulated data around the instance by random perturbation, and obtaining feature importance through applying some form of feature selection. While LIME and similar local algorithms have gained popularity due to their simplicity, the random perturbation and feature selection methods result in "instability" in the generated explanations, where for the same prediction, different explanations can be generated. This is a critical issue that can prevent deployment of LIME in a Computer-Aided Diagnosis (CAD) system, where stability is of utmost importance to earn the trust of medical professionals. In this paper, we propose a deterministic version of LIME. Instead of random perturbation, we utilize agglomerative Hierarchical Clustering (HC) to group the training data together and K-Nearest Neighbour (KNN) to select the relevant cluster of the new instance that is being explained. After finding the relevant cluster, a linear model is trained over the selected cluster to generate the explanations. Experimental results on three different medical datasets show the superiority for Deterministic Local Interpretable Model-Agnostic Explanations (DLIME), where we quantitatively determine the stability of DLIME compared to LIME utilizing the Jaccard similarity among multiple generated explanations.</p>
The Histidine Kinase Hik34 Is Involved in Thermotolerance by Regulating the Expression of Heat Shock Genes in Synechocystis (2005)

Histidine kinases (Hiks) in Synechocystis sp. PCC 6803 are involved in the transduction of signals associated with various kinds of environmental stress. To examine the potential role in thermotolerance of Hiks, we used genome microarray analysis to screen a Hik knockout library for mutations that affected the expression of genes for heat shock proteins. Mutation of the hik34 gene enhanced the levels of transcripts of a number of heat shock genes, including htpG and groESL1. Overexpression of the hik34 gene repressed the expression of these heat shock genes. In addition, the cells with a mutant gene for Hik34 (DeltaHik34 cells) survived incubation at 48 degrees C for 3 h, while wild-type cells and cells with mutations in other Hiks were killed. However, mutation of the hik34 gene had only an insignificant effect on the global expression of genes upon incubation of the mutant cells at 44 degrees C for 20 min. Quantitative two-dimensional gel electrophoresis revealed that levels of GroES and HspA were elevated in DeltaHik34 cells after incubation of cells at 42 degrees C for 60 min. We overexpressed recombinant Hik34 protein in Escherichia coli and purified it. We found that the protein was autophosphorylated in vitro at physiological temperatures, but not at elevated temperatures, such as 44 degrees C. These results suggest that Hik34 might negatively regulate the expression of certain heat shock genes that might be related to thermotolerance in Synechocystis.
Genetic Engineering for Enhancing Plant Productivity and Stress Tolerance (2004)

Yield enhancement of crops has been the most important goal in agriculture, and genetic manipulations in plants are pivotal in this effort. Until the early 1980s, traditional breeding had been virtually the only effort in altering plant genetic makeup so that desirable agricultural traits could be obtained. Although these breeding efforts have generated numerous new plant varieties that contribute enormously to the success of agriculture, recent advances in genetic engineering based on recombinant DNA and plant transformation technologies have allowed a much faster and more precise approach in manipulating plant genetics. Furthermore, the genetic engineering approach is not limited to introducing genes among plant species that can be cross-pollinated. Virtually any genes from diverse sources can be introduced to desirable plants. Both the Agrobacterium-based and biolistic plant transformation techniques have been well developed, and most crops, including wheat, rice, maize, soybean, and potatoes, are now routinely transformed by using these methods. These technical advancements, in conjunction with the vast amount of knowledge gained in plant biochemistry and physiology, have allowed researchers to alter plant functions by over-or underexpressing specific genes involved in key metabolic pathways, so that desirable metabolites are accumulated for the enhancement of plant productivity.
Vehicle Detection and Collision Avoidance System (2022)

Over past few decades in India, there is drastic increase in vehicle usage for conveyance. Increasement in vehicle usage for transportation routes to traffic congestion and frequent road accidents. Usually not following traffic rules and road abnormalities are the few reasons for accidents. Vehicles encounter into collision due to error done by the drivers and accident occurs due to bad weather related situations. Irrespective of lot of current improvements in technological inventions available for vehicle safety, increase in accidents occurs every day. Hence, a suitable method for vehicle collision detection and avoidance is essential. So, this literature survey impacts the study on various approaches available towards and need for vehicle collision detection and collision avoidance system.
Modulation of human estrogen receptor α activity by multivalent estradiol–peptidomimetic conjugates (2011)

Estradiol-peptidomimetic conjugates (EPCs) are linear, sequence-specific peptoid oligomers that site-specifically display multiple copies of 17β-estradiol (E2), a ligand for the human estrogen receptor α (hERα). We evaluate the ability of multivalent EPCs to activate hERα-mediated transcription. EPCs activated the hERα in both a length- and valence-dependent manner, with the highest levels of activation generated by divalent peptoid 6-mers, divalent 18-mers, and trivalent 9-mers. Hexavalent EPCs did not activate hERα, but instead blocked E2-mediated hERα activation. The physicochemical features of EPCs can be precisely tuned, which may allow the generation of a library of chemical tools for modulating specific effects of estrogens.
Human Stx2-Specific Monoclonal Antibodies Prevent Systemic Complications of <i>Escherichia coli</i> O157:H7 Infection (2002)

Hemolytic-uremic syndrome (HUS) is a serious complication predominantly associated with infection by enterohemorrhagic Escherichia coli (EHEC), such as E. coli O157:H7. EHEC can produce Shiga toxin 1 (Stx1) and/or Shiga toxin 2 (Stx2), both of which are exotoxins comprised of active (A) and binding (B) subunits. In piglets and mice, Stx can induce fatal neurological symptoms. Polyclonal Stx2 antiserum can prevent these effects in piglets infected with the Stx2-producing E. coli O157:H7 strain 86-24. Human monoclonal antibodies (HuMAbs) against Stx2 were developed as potential passive immunotherapeutic reagents for the prevention and/or treatment of HUS. Transgenic mice bearing unrearranged human immunoglobulin (Ig) heavy and kappa light chain loci (HuMAb___Mouse) were immunized with formalin-inactivated Stx2. Thirty-seven stable hybridomas secreting Stx2-specific HuMAbs were isolated: 33 IgG1kappa A-subunit-specific and 3 IgG1kappa and 1 IgG3kappa B-subunit-specific antibodies. Six IgG1kappa A-subunit-specific (1G3, 2F10, 3E9, 4H9, 5A4, and 5C12) and two IgG1kappa B-subunit-specific (5H8 and 6G3) HuMAbs demonstrated neutralization of > 95% activity of 1 ng of Stx2 in the presence of 0.04 microg of HuMAb in vitro and significant prolongation of survival of mice given 50 microg of HuMAb intraperitoneally (i.p.) and 25 ng of Stx2 intravenously. When administered i.p. to gnotobiotic piglets 6 or 12 h after infection with E. coli O157:H7 strain 86-24, HuMAbs 2F10, 3E9, 5H8, and 5C12 prolonged survival and prevented development of fatal neurological signs and cerebral lesions. The Stx2-neutralizing ability of these HuMAbs could potentially be used clinically to passively protect against HUS development in individuals infected with Stx-producing bacteria, including E. coli O157:H7.
ACG Clinical Guideline: Small Intestinal Bacterial Overgrowth (2020)

Small intestinal bacterial overgrowth is defined as the presence of excessive numbers of bacteria in the small bowel, causing gastrointestinal symptoms. This guideline statement evaluates criteria for diagnosis, defines the optimal methods for diagnostic testing, and summarizes treatment options for small intestinal bacterial overgrowth. This guideline provides an evidence-based evaluation of the literature through the Grading of Recommendations Assessment, Development, and Evaluation (GRADE) process. In instances where the available evidence was not appropriate for a formal GRADE recommendation, key concepts were developed using expert consensus.
Ultrasensitive Hydrogen Sensor Based on Pd<sup>0</sup>-Loaded SnO<sub>2</sub>Electrospun Nanofibers at Room Temperature (2013)

Pd(0)-loaded SnO2 nanofibers have been successfully synthesized with different loaded levels via electrospinning process, sintering technology, and in situ reduction. This simple strategy could be expected to extend for the fabrication of similar metal-oxide loaded nanofibers using different precursors. The morphological and structural characteristics of the resultant product were investigated by scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), and X-ray photoelectron spectra (XPS). To demonstrate the usage of such Pd(0)-loaded SnO2 nanomaterial, a chemical gas sensor has been fabricated and investigated for H2 detection. The sensing performances versus Pd(0)-loaded levels have been investigated in detail. An ultralow limit of detection (20 ppb), high response, fast response and recovery, and selectivity have been obtained on the basis of the sensors operating at room temperature. The combination of SnO2 crystal structure and catalytic activity of Pd(0)-loaded gives a very attractive sensing behavior for applications as real-time monitoring gas sensors.
Customisable Core-Based Architectures for Real-Time Motion Estimation on FPGAs (2008)

Abstract. This paper proposes new core-based architectures for motion estimation that are customisable for different coding parameters and hardware resources. These new cores are derived from an efficient and fully parameterisable 2-D single array systolic structure for full-search block-matching motion estimation and inherit its configurability properties in what concerns the macroblock dimension, the search area and parallelism level. The proposed architectures require significantly fewer hardware resources, by reducing the spatial and pixel resolutions rather than restricting the set of considered candidate motion vectors. Low-cost and low-power regular architectures suitable for field programmable logic implementation are obtained without compromising the quality of the coded video sequences. Experimental results show that despite the significant complexity level presented by motion estimation processors, it is still possible to implement fast and low-cost versions of the original core-based architecture using general purpose FPGA devices. 1
[Molecular genetic mechanism of sodium arsenite on growth and development]. (2004)

The sodium arsenite could affect the gene expression related to growth and development and it is shown that the molecular genetic mechanism of sodium arsenite is related to growth and development.
A new fluorescence staining assay for visualizing living microorganisms in soil (1995)

5- (and 6-)Sulfofluorescein diacetate (SFDA), which is converted to a fluorescent product by intracellular esterase activity, was used to stain living microorganisms, including bacteria, Saccharomyces cerevisiae, and fungi, in soil. SFDA (1 mM) dissolved in ethyl alcohol was added to an intact soil sample, and the preparation was examined with an epifluorescence microscope. Bright single cells and colonies of live bacteria were observed without interference from the autofluorescence of soil minerals and detritus. Cultured Escherichia coli was killed through heat treatment; thus, SFDA was concluded to stain only living cells. Microbial colonies obtained from natural soils and various cultured strains were tested. It was found that 151 of 154 colonies from natural soils were stained and that hyphae and spores from 1 of 28 cultured microbial strains were not stained. The SFDA method was successfully used to visualize and count bacteria in soil samples from Mount Shiga in Japan.
Characterization of kinetic limitations to atmospheric CO<sub>2</sub> capture by solid sorbent (2015)

Abstract Scrubbing CO 2 directly from the ambient air (air capture) by sorbent is an emerging technology to address climate change due to anthropic emission of greenhouse gases. A good understanding of thermodynamics and reaction kinetics under ultra‐low CO 2 concentration and variable humidity is fundamental to the design of economic air capture. In this study, the adsorption kinetics of a novel moisture swing process for air capture is characterized. The variation of humidity alters not only the adsorption equilibrium but also the adsorption kinetics, due to a water involved chemical reaction. A heterogeneously structured sorbent sheet with strong based ion exchange resin inside as active constituent is employed to capture the atmospheric CO 2 under different humidity. Traditional shrinking core model (SCM) is modified to reveal the mass transfer resistance inside of the sorbent for CO 2 adsorption under ultra‐low CO 2 concentration. The modified SCM reveals that, at most cases, the adsorption kinetics is controlled by diffusion inside of the sorbent particle. A transition from physical diffusion controlled kinetics to chemical reaction controlled kinetics is observed as humidity increases. Further kinetic analysis suggests that, among the optimization technologies such as seeking functional group with faster chemical reaction or fabricating thinner support layer, preparing sorbent particle with smaller size or higher porosity should have much more potential on the enhancement of air capture kinetics. © 2015 Society of Chemical Industry and John Wiley & Sons, Ltd